Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources.
A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from polysaccharide- and proteoglycan-rich material. In the modified procedure, RNA is precipitated from the aqueous phase by the combined action of isopropanol and a high-salt concentration. Under these conditions, RNA is effectively precipitated while contaminating polysaccharides and proteoglycans remain in the soluble form. The modified precipitation does not prolong or increase the complexity of the TRI Reagent procedure. The new procedure was tested by isolation of RNA from polysaccharide- and proteoglycan-rich tissues such as rat liver and aorta.
Chomczynski
P
P
College of Medicine, University of Cincinnati, OH, USA.
Mackey
K
K
D000433:1-Propanol; D000818:Animals; D002478:Cells, Cultured; D007202:Indicators and Reagents; D008967:Molecular Biology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011134:Polysaccharides / Q000032:analysis*; D011509:Proteoglycans / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase; D051381:Rats
D007202:Indicators and Reagents; D011134:Polysaccharides; D011509:Proteoglycans; D012313:RNA; D000433:1-Propanol; D012194:RNA-Directed DNA Polymerase