Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli.
The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild-type yeast as feedback regulator of sterol biosynthesis.
Venkatramesh
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M
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409, USA.
Guo
D A
DA
Harman
J G
JG
Nes
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WD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D005800:Genes, Fungal; D007700:Kinetics; D008780:Methyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013261:Sterols / Q000378:metabolism*; D013379:Substrate Specificity
D012333:RNA, Messenger; D011994:Recombinant Proteins; D013261:Sterols; D008780:Methyltransferases; C022670:delta 24-sterol methyltransferase