title: Distribution of p75 and trk-neurotrophin receptor proteins in adult human sympathetic ganglia.
abstract: We investigated the expression of immunoreactivity (IR) for low- (p75) and high-affinity (trk proteins) neurotrophin-receptor proteins in adult human paravertebral-sympathetic ganglion neurons. Mouse monoclonal antibodies against the pan-neurotrophin-receptor p75, and rabbit polyclonal antibodies against specific epitopes of the intracytoplasmic domain on trk neurotrophin-receptor proteins were used in fresh unfixed and formaldehyde-fixed paraffin-embedded sympathetic ganglia. All adult human paravertebral-sympathetic neurons displayed trkA neurotrophin-receptor-like protein IR, 10% express trkC neurotrophin-receptor-like protein IR, 37-44% show p75 IR, and no IR was obtained for trkB neurotrophin-receptor-like protein. The intensity of immunostaining was independent of the neuron size. Labelling of non-neuronal tissues, especially blood-vessel walls, was observed for p75, trkA and trkC neurotrophin-receptor proteins. These results indicate that overlapping exists in the expression of p75 and trk neurotrophin-receptor proteins in adult human paravertebral-sympathetic neurons, and suggest that neurotrophins might act on these neurons.
authors: Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain.
authors: O
authors: O
authors: Garcia-Suarez
authors: F J
authors: FJ
authors: Naves
authors: M E
authors: ME
authors: Del Valle
authors: I
authors: I
authors: Esteban
authors: E
authors: E
authors: Bronzetti
authors: E
authors: E
authors: Vazquez
authors: J A
authors: JA
authors: Vega
mesh_terms: D000328:Adult; D000367:Age Factors; D000918:Antibody Specificity; D005260:Female; D005728:Ganglia, Sympathetic / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry*; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000276:immunology; D020801:Receptor, Ciliary Neurotrophic Factor; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000032:analysis / Q000276:immunology; D020812:Receptor, trkC; D017475:Receptors, Nerve Growth Factor / Q000032:analysis* / Q000276:immunology
chemical_list: D020801:Receptor, Ciliary Neurotrophic Factor; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA; D020812:Receptor, trkC