[Ca2+]i regulation by glutamate receptor agonists in cultured chick retina cells.
The effect of glutamate receptor agonists on the intracellular free calcium concentration ([Ca2+]i), measured with Indo-1, was studied in populations of cultured chick embryonic retina cells. The agonists of the ionotropic glutamate receptors, N-methyl-D-aspartate (NMDA), kainate, and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) increased the [Ca2+]i through a composite effect, comprising Ca2+ permeating the receptor-associated channels, and Ca2+ entering through voltage-gated Ca2+ channels. Furthermore, the [Ca2+]i responses to NMDA and AMPA also involved Ca2+ release from intracellular stores, which could not be mobilized by stimulation of the metabotropic receptor.
Duarte
C B
CB
Department of Zoology, University of Coimbra, Portugal.
Santos
P F
PF
Carvalho
A P
AP
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D015220:Calcium Channels; D018690:Excitatory Amino Acid Agonists; D007295:Inositol Phosphates; D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007608:Kainic Acid; D002118:Calcium