title: Granulocyte/macrophage-colony-stimulating factor induces the migration of human epidermal Langerhans cells in vitro.
abstract: In the present study, we investigated the in vitro migratory activity of human epidermal Langerhans cells (LC). Freshly isolated LC exhibit very low spontaneous migration. In contrast, a strong migration is recorded 6 h after the isolation. This migration is due to the presence of GM-CSF released by surrounding keratinocytes in vitro. Picomolar concentrations of GM-CSF promote the migration of LC, but nanomolar concentrations are inhibitory. Checker-board experiments indicate that GM-CSF acts as a chemokinetic mediator for LC, Bulk cultured LC exhibit a significant decrease of their spontaneous migration but retain the capacity to respond to GM-CSF only at nanomolar concentrations. In contrast, LC cultured in the presence of picomolar concentrations of exogenous GM-CSF exhibit a spontaneous migratory activity comparable to that of 6 h rested LC but do not respond to GM-CSF. These results suggest that GM-CSF represents an essential factor triggering the egress of LC from their epidermal environment.
authors: Department of Dermatology, Ludwig-Maximilians University, Munich, Germany.
authors: R
authors: R
authors: Rupec
authors: R
authors: R
authors: Magerstaedt
authors: C G
authors: CG
authors: Schirren
authors: E
authors: E
authors: Sander
authors: T
authors: T
authors: Bieber
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D007700:Kinetics; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor