title: Differential regulation of phenylethanolamine N-methyltransferase expression in two distinct subpopulations of bovine chromaffin cells.
abstract: Chromaffin cells were isolated from bovine adrenal glands and fractionated into two distinct subpopulations by density gradient centrifugation on Percoll. Cells in the more dense fraction stored epinephrine (E) as their predominant catecholamine (81% of total catecholamines), contained high levels of phenylethanolamine N-methyltransferase (PNMT) activity, and exhibited intense PNMT immunoreactivity. This population of chromaffin cells was termed the E-rich cell population. Cells in the less dense fraction, the norepinephrine (NE)-rich cell population, stored predominantly NE (75% of total catecholamines). Although the NE-rich cells had only 3% as much PNMT activity as did the E-rich cells, 20% of the NE-rich cells were PNMT immunoreactive. This suggested that the PNMT-positive cells in the NE-rich cell cultures contained less PNMT per cell than did E-rich cells and may not be typical adrenergic cells. The regulation of PNMT mRNA levels and PNMT activity in primary cultures of E-rich and NE-rich cells was compared. At the time the cells were isolated, PNMT mRNA levels in NE-rich cells were approximately 20% of those in E-rich cells; within 48 h in culture, PNMT mRNA in both populations declined to almost undetectable levels. Treatment with dexamethasone increased PNMT mRNA levels and PNMT activity in both populations. In E-rich cells, dexamethasone restored PNMT mRNA to the level seen in freshly isolated cells and increased PNMT activity twofold. In NE-rich cells, dexamethasone increased PNMT mRNA to levels twice those found in freshly isolated cells and increased PNMT activity sixfold. Cycloheximide blocked the effects of dexamethasone on PNMT mRNA expression in NE-rich cells but had little effect in E-rich cells. Angiotensin II, forskolin, and phorbol 12,13-dibutyrate elicited large increases in PNMT mRNA levels in E-rich cells but had no effect in NE-rich cells. Our data suggest that PNMT expression is regulated differently in the two chromaffin cell subpopulations.
authors: Department of Pediatrics, University of Chicago, IL 60637, USA.
authors: A L
authors: AL
authors: Cahill
authors: A L
authors: AL
authors: Eertmoed
authors: D
authors: D
authors: Mangoura
authors: R L
authors: RL
authors: Perlman
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000201:enzymology; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D002498:Centrifugation; D002838:Chromaffin System / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D003102:Colloids; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004837:Epinephrine / Q000032:analysis / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism; D010625:Phenylethanolamine N-Methyltransferase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011205:Povidone; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012822:Silicon Dioxide; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D003102:Colloids; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; C016039:Percoll; D012822:Silicon Dioxide; D003907:Dexamethasone; D003513:Cycloheximide; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase; D011205:Povidone; D009638:Norepinephrine; D004837:Epinephrine