Lack of evidence of oxidative damage in antioxidant-deficient strains of Saccharomyces cerevisiae.
The content of reactive carbonyls and of glutathione-protein mixed disulfides, two indices of oxidative stress, were compared in wild-type Saccharomyces cerevisiae and in strains deficient in superoxide dismutase and catalase, and of decreased glutathione level. Both indices were higher in stationary than in logarithmic cultures and were not increased in antioxidant-deficient strains. Oxidation of dichlorofluorescin, an estimate of peroxide production, measured in the presence of exogenous peroxidase, was higher in antioxidant-deficient strains. These results corroborate our previous results on compensatory antioxidant mechanisms in the mutant yeast strains.
Fortuniak
A
A
Department of Molecular Biophysics, University of Lódź, Poland. afort@biol.uni.lodz.pl
Jakubowski
W
W
Biliński
T
T
Bartosz
G
G
D020642:Acatalasia; D000975:Antioxidants / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000172:deficiency*
D000975:Antioxidants; D004220:Disulfides; D005656:Fungal Proteins; D013482:Superoxide Dismutase; D005978:Glutathione