Bradykinin upregulates immediate-early gene mRNA in human keratinocytes.
We investigated the effect of bradykinin on the expression of the proto-oncogenes c-fos, c-jun and c-myc and on cell proliferation in the human keratinocyte cell line, HaCaT. Analysis of mRNAs was done by Northern blotting with single-stranded DNA hybridization probes. Bradykinin caused a rapid and transient accumulation in c-fos and c-jun mRNAs. In contrast, c-myc mRNA increased more slowly. Moreover, we report that bradykinin was a weak stimulator of HaCaT cell proliferation whereas epidermal growth factor, which induced the same degree of mRNA elevation, was shown as a powerful mitogen. Thus, while in HaCaT cells bradykinin promotes the expression of the proto-oncogenes c-fos, c-jun and c-myc, other biochemical events appear to be necessary for cell division.
Coutant
K D
KD
Sandoz Research Institute, Dermatology Department, Vienna, Austria.
Ryder
N S
NS
D001483:Base Sequence; D001920:Bradykinin / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D017781:Genes, Immediate-Early / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D011519:Proto-Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D001920:Bradykinin