Topical vitamin D3 downregulates IgE-mediated murine biphasic cutaneous reactions.
Hapten-specific and mast-cell-dependent biphasic (immediate and delayed-onset) cutaneous reactions were induced in a murine model by intravenous injection of anti-DNP IgE antibodies followed by a skin test. Four daily applications of topical 1 alpha,24(OH)2D3 ointment significantly inhibited both the immediate and the delayed-onset cutaneous reactions in a dose-dependent fashion, as well as the croton-oil-induced cutaneous reaction and DNFB contact sensitivity reaction. 1 alpha,24(OH)2D3 itself did not show any sensitizing or irritant potential. The inhibitory effect of 1 alpha,24(OH)2D3 on these reactions was limited to the application site and no systemic effect was observed. Another vitamin D3 analog 1 alpha,25(OH)2D3, also showed an inhibitory effect on IgE-mediated cutaneous reactions. These results suggest that topically applied 1 alpha,24(OH)2D3 might modulate IgE-mediated cutaneous reactions and could thus be useful in the treatment of certain human cutaneous disorders other than psoriasis and related keratinizing disorders.
Katayama
I
I
Department of Dermatology, Tokyo Medical and Dental University, School of Medicine, Japan.
Minatohara
K
K
Yokozeki
H
H
Nishioka
K
K
D000287:Administration, Topical; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D003436:Croton Oil / Q000276:immunology; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004137:Dinitrochlorobenzene / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004431:Ear, External / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D004137:Dinitrochlorobenzene; D004140:Dinitrophenols; D006241:Haptens; D002762:Cholecalciferol; D007073:Immunoglobulin E; D003436:Croton Oil; D004139:Dinitrofluorobenzene