title: Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis.
abstract: OBJECTIVE
  To determine if embryos can modulate steroid hormone production by luteinized granulosa cells.
  
  
  METHODS
  Granulosa cells obtained from follicular aspirates were cultured alone or in the presence of a two-pronuclear zygote. The production of E2 and P by these cultures was evaluated by RIA.
  
  
  METHODS
  In Vitro Fertilization Unit in an academic research environment.
  
  
  METHODS
  Sixteen women undergoing IVF.
  
  
  METHODS
  Standard IVF-ET treatment cycle using leuprolide acetate for pituitary desensitization before hMG or urofollitropin for ovarian stimulation.
  
  
  METHODS
  Estradiol and P concentration in culture media of luteinized granulosa cells alone or granulosa cells cocultured with a two-pronuclear embryo.
  
  
  RESULTS
  Both E2 and P production by luteinized granulosa cells was reduced when cultured in the presence of an embryo.
  
  
  CONCLUSIONS
  Human embryos secrete a factor that regulates granulosa cell steroidogenesis.
authors: Brown University of School of Medicine, Women's and Infants Hospital of Rhode Island, Providence, USA.
authors: D B
authors: DB
authors: Seifer
authors: M R
authors: MR
authors: Freeman
authors: A C
authors: AC
authors: Gardiner
authors: G A
authors: GA
authors: Hill
authors: A L
authors: AL
authors: Schneyer
authors: B C
authors: BC
authors: Vanderhyden
mesh_terms: D000328:Adult; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D018920:Coculture Techniques / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004967:Estrogens / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016729:Leuprolide / Q000494:pharmacology; D011374:Progesterone / Q000032:analysis / Q000096:biosynthesis*; D011863:Radioimmunoassay; D015053:Zygote / Q000166:cytology / Q000502:physiology*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D003470:Culture Media; D004967:Estrogens; D011374:Progesterone; D016729:Leuprolide