[Effects of halothane, isoflurane, enflurane, and sevoflurane on the monosynaptic reflex response in the isolated spinal cord of newborn rats].
Monosynaptic reflex responses (MSRs) in the isolated spinal cord of newborn rats were elicited in the ventral root by stimulation of the ipsilateral dorsal root. MSRs were considered to be mediated by non-NMDA class glutamate receptors. We studied the depressant effects of halothane, isoflurane, enflurane, and sevoflurane on MSR amplitudes as a function of anesthetic concentration comparing with MAC value of each anesthetics. The spinal cord of newborn rats were rapidly dissected out, and placed in a chamber superfused with artificial cerebrospinal fluid (ACSF, pH 7.4, 34 degrees C) equilibrated with 95% O2 - 5% CO2. Anesthetic gases were delivered with specific vaporizers and bubbled in the ACSF. Each anesthetic concentration was determined by gas chromatography. Either of the anesthetics reversibly depressed MSR amplitudes in a concentration dependent fashion. Concentration-response curves for MSR amplitudes were constructed and the concentrations which produced a half-maximum inhibition (IC50) were 0.56, 0.65, 0.97 and 1.18 mM for halothane, isoflurane, enflurane, and sevoflurane, respectively. These IC50 values correlated well with those of MAC values (r = 0.999, P < 0.001) obtained from adult rats in an in vivo condition. The MSR response in the isolated spinal cord of newborn rats is considered as a useful model for analysis of potency of volatile anesthetics.
Tsutahara
S
S
Department of Anesthesiology, Hokkaido University School of Medicine, Sapporo.
Furumido
H
H
Ohta
Y
Y
Harasawa
K
K
Yamamura
T
T
Kemmotsu
O
O
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D004737:Enflurane / Q000494:pharmacology*; D004987:Ethers / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology*; D066298:In Vitro Techniques; D007530:Isoflurane / Q000494:pharmacology*; D008738:Methyl Ethers; D051381:Rats; D017208:Rats, Wistar; D012023:Reflex, Monosynaptic / Q000187:drug effects*; D000077149:Sevoflurane; D013116:Spinal Cord / Q000187:drug effects*
D018685:Anesthetics, Inhalation; D004987:Ethers; D008738:Methyl Ethers; D000077149:Sevoflurane; D004737:Enflurane; D007530:Isoflurane; D006221:Halothane