Assignment of the crystal toxin genes of the mosquitocidal bacterium, Bacillus thuringiensis israelensis to a specific plasmid.
Bacillus thuringiensis israelensis 4Q1-WT and the prepared mutants, 4Q1-72 and 4Q1-81 were bioassayed against Aedes caspius larvae. The strain -4Q1-WT, which contains all plasmid arrys and the strain 4Q1-72 which contains the 108 kb plasmid gave 100% mortality, while stain 4Q1-81 which contains no plasmids gave 0% mortality. Crystals from all tested strains were isolated, solubilized and characterized using PAGE to detect any homology or difference in crystal production, properties, and the relatedness to the plasmid profile.
Rady
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Department of Entomology, Faculty of Science, Ain Shams University, Cairo.
Ramadan
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D000330:Aedes; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D001427:Bacterial Toxins; D001681:Biological Assay; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D007814:Larva; D010572:Pest Control, Biological; D010957:Plasmids
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis