Leukocyte trafficking in free-flowing cerebrospinal fluid of normal rhesus macaques (Macaca mulatta).
Cellular components in free-flowing cerebrospinal fluid (CSF) of normal rhesus macaques were characterized. Microscopic counting enumerated the total number of leukocytes, percentage of polymorphonuclear cells (PMN), leukocytes with nonspecific esterase (NSE), and those reducing nitro blue tetrazolium (NBT). Flow cytometric analysis further identified CD4, CD8, CD14, and CD20 positive leukocytes. These experiments established reliable techniques for evaluating cellular components in CSF from rhesus macaques and documented the difference in the CD4/CD8 ratio between peripheral blood (PB) and CSF compartments under normal physiological conditions.
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Department of Zoology, University of Wisconsin-Madison 53715, USA.
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Erickson
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D000818:Animals; D018951:Antigens, CD20 / Q000097:blood / Q000134:cerebrospinal fluid; D015415:Biomarkers; D015704:CD4 Antigens / Q000097:blood / Q000134:cerebrospinal fluid; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000097:blood / Q000134:cerebrospinal fluid; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005434:Flow Cytometry; D007962:Leukocytes / Q000166:cytology*; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000134:cerebrospinal fluid; D008253:Macaca mulatta; D009504:Neutrophils / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D018951:Antigens, CD20; D015415:Biomarkers; D015704:CD4 Antigens; D016827:CD8 Antigens; D018950:Lipopolysaccharide Receptors; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase