The effect of nociceptin on Ca2+ channel current and intracellular Ca2+ in the SH-SY5Y human neuroblastoma cell line.
The human neuroblastoma cell line SH-SY5Y expresses the 'orphan' opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration-dependent inhibition of the N-type calcium channel current in these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 microM), naltrindole (1 microM) and naloxone (10 microM).
Connor
M
M
Department of Pharmacology, University of Bristol.
Yeo
A
A
Henderson
G
G
D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009292:Narcotic Antagonists / Q000494:pharmacology; D009447:Neuroblastoma; D018847:Opioid Peptides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D037342:Pertussis Toxin; D011957:Receptors, Opioid / Q000819:agonists; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D000097629:Nociceptin
D002118:Calcium; D002121:Calcium Channel Blockers; D002217:Carbachol; D004791:Enzyme Inhibitors; D009292:Narcotic Antagonists; D018847:Opioid Peptides; D037342:Pertussis Toxin; D011957:Receptors, Opioid; D010566:Virulence Factors, Bordetella; D000097629:Nociceptin