Assessing genetic-based therapies for AIDS using the simian immunodeficiency virus.
A plasmid encoding the full-length infectious molecular proviral clone of SIVmac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4+ cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co-transfection studies were initiated with the infectious proviral clone and a well-characterized trans-dominant negative HIV-1 rev mutant.
Endres
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CL
Division of Pathobiology and Immunology, Oregon Regional Primate Research Center, Beaverton, USA.
Bergquam
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E
Axthelm
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MK
Wong
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SW
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D018014:Gene Transfer Techniques; D015757:Genes, rev; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008253:Macaca mulatta; D009154:Mutation; D010957:Plasmids; D011533:Proviruses / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D013997:Time Factors; D014162:Transfection; D014779:Virus Replication