Longitudinal study of molecular epidemiology of small round-structured viruses in a pediatric population.
Small round-structured viruses (SRSV), recently designated members of the family Caliciviridae, can now be readily subtyped by amplification of a defined portion of their genome by reverse transcription-PCR and then by identification of the amplicons with specific probes by Southern blotting. A longitudinal survey (from 1991 to 1995) was conducted to determine the genotypes of the SRSV present in pediatric stool specimens from patients with sporadic cases of gastroenteritis. It was found that almost all viruses were of the G-2 genotype, and on probing, the subtype P2-B was predominant but the frequencies of the different subtypes varied from year to year. A survey of the genotypes of SRSV from community outbreaks from 1995 showed that the G-2 genotype was also predominant and that the distribution of its subtypes was similar to that seen in sporadic cases of diarrhea in pediatric patients over the same time period. It was concluded that there is a succession of subtypes of SRSV in our pediatric population over time. This distribution of genotypes in sporadic cases of pediatric gastroenteritis may reflect the distribution in community outbreaks occurring at the same time.
Levett
P N
PN
Department of Microbiology, Hospital for Sick Children, and Laboratory Services Branch, Ontario Ministry of Health, Toronto, Ontario, Canada.
Gu
M
M
Luan
B
B
Fearon
M
M
Stubberfield
J
J
Jamieson
F
F
Petric
M
M
D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008137:Longitudinal Studies; D017720:Molecular Epidemiology; D009663:Norwalk virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012621:Seasons
D012367:RNA, Viral