Endothelin-1 mediates erythropoietin-stimulated glomerular endothelial cell-dependent proliferation of mesangial cells.
These experiments were performed in an attempt to determine whether chronic stimulation of glomerular endothelial cells with recombinant human erythropoietin would alter mesangial cell proliferation. Glomerular endothelial cells in culture incubated with various concentrations of erythropoietin for up to 4 days exhibited dose-dependent endothelin-1 production. Moreover, the conditioned medium from erythropoietin-stimulated glomerular endothelial cells enhanced [3H]thymidine incorporation into mesangial cells. This enhancement was significantly attenuated in the presence of a endothelin A receptor antagonist, BQ-123. These results suggest that endothelin-1 mediates erythropoietin-stimulated glomerular endothelial cell-dependent mesangial cell proliferation, resulting in the progression of glomerulonephritis.
Nitta
K
K
Department of Medicine, Tokyo Women's Medical College, Japan.
Uchida
K
K
Kimata
N
N
Kawashima
A
A
Yumura
W
W
Nihei
H
H
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D016232:Endothelins / Q000502:physiology*; D004727:Endothelium / Q000187:drug effects / Q000502:physiology*; D004921:Erythropoietin / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000502:physiology*; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology
D016232:Endothelins; D011994:Recombinant Proteins; D004921:Erythropoietin