[The transport of enoxacin in cultured kidney epithelial cells LLC-PK1].
In this study, the transport of enoxacin (ENX) was investigated in a LLC-PK1 kidney epithelial cell line. The uptake of ENX by LLC-PK1 monolayers cultured in plastic dishes was shown to be temperature-dependent and concentration-dependent. Cimetidine and guanidine inhibited the uptake of ENX, whereas TEA and NMN did not. The basolateral to apical flux of ENX across LLC-PK1 monolayers cultured on permeable supports was about two times larger than the apical to basolateral flux. The basolateral to apical flux of ENX was remarkably inhibited by guanidine, whereas it was not inhibited by TEA, NMN and cimetidine. The apical to basolateral flux of ENX was inhibited by cimetidine and guanidine, whereas it was not inhibited by TEA and NMN.
Sasaya
M
M
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Shida
M
M
Hirano
T
T
Oda
M
M
Endo
T
T
Sakata
M
M
Miyazaki
S
S
Takada
M
M
D000818:Animals; D001692:Biological Transport; D002927:Cimetidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015365:Enoxacin / Q000493:pharmacokinetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D018374:LLC-PK1 Cells; D013552:Swine; D013696:Temperature
D006146:Guanidines; D015365:Enoxacin; D002927:Cimetidine; D019791:Guanidine