Deacylation of influenza virus hemagglutinin does not affect the kinetics of low pH induced membrane fusion.
The relevance of palmitoylation of cysteine residues of influenza virus hemagglutinin (HA) for the HA-mediated membrane fusion triggered at low pH is investigated. Either wild-type HA (subtype H7) or mutant HA devoid of fatty acids were expressed in insect cells. The kinetics as well as the extent of fusion of HA-expressing cells with human erythrocyte ghosts were measured by a membrane mixing assay. Fusion was measured continuously at different pH by fluorescence dequenching of the lipid-like fluorophore R18 initially incorporated into the erythrocyte membrane. No significant difference between fusion of wild-type and mutant HA expressing cells with ghosts could be detected showing that deacylation does affect neither the extent nor the kinetics of fusion.
Schroth
B
B
Institut für Biologie und Biophysik, Mathematisch-Naturwissenschaftliche Fakulrät I. Humboldt-Universität zu Berlin, Germany.
Philipp
H C
HC
Veit
M
M
Schmidt
M F
MF
Herrmann
A
A
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D003640:Dealkylation; D004910:Erythrocyte Membrane / Q000378:metabolism; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D007313:Insecta; D007700:Kinetics; D008561:Membrane Fusion / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D006388:Hemagglutinins