Scavenging effects of salvia miltiorrhiza on free radicals and its protection for myocardial mitochondrial membranes from ischemia-reperfusion injury.
Using low temperature electron spin resonance (ESR) technique, we found that Salvia miltiorrhiza injection could scavenge the oxygen free radicals generated from ischemia-reperfusion injury in the myocardium as effectively as SOD. Using ESR spin trapping technique we found that one of its effective components, Danshensu, could scavenge superoxide anion free radicals generated from the reaction system of xanthine and xanthine oxidase, and that lipid free radicals generated from lipid peroxidation of myocardial mitochondrial membranes could be scavenged by another effective component, Tanshinone. The membrane fluidity of the mitochondria isolated from the ischemia-reperfused hearts was studied with the ESR spin labelling technique, and the TBA-method was used to detect the lipid peroxidation. It was found that Danshensu could protect the mitochondrial membrane from the ischemia-reperfusion injury and lipid peroxidation.
Zhao
B L
BL
Institute of Biophysics, Academia Sinica, Beijing, China.
Jiang
W
W
Zhao
Y
Y
Hou
J W
JW
Xin
W J
WJ
D045784:Abietanes; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects*; D007773:Lactates / Q000494:pharmacology; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology; D010936:Plant Extracts; D011817:Rabbits; D027561:Salvia miltiorrhiza; D019097:Spin Trapping; D013481:Superoxides / Q000032:analysis / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism
D045784:Abietanes; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D007773:Lactates; D010616:Phenanthrenes; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C021751:tanshinone; D013481:Superoxides; C035055:3,4-dihydroxyphenyllactic acid