Protective effects of macrophage-derived interferon against encephalomyocarditis virus-induced diabetes mellitus in mice.
The involvement of macrophages in protection against diabetes mellitus in mice of BALB/c (susceptible) and C57BL (resistant) strains infected with the B (non-diabetogenic) or D (highly diabetogenic) variant of encephalomyocarditis (EMC) virus was examined. Pretreatment with the B variant of EMC virus (EMC-B), avirulent interferon (IFN) inducer, or Corynebacterium parvum inhibited diabetes in BALB/c mice infected with the D variant of EMC virus (EMC-D). Treatment of C57BL mice with carrageenan to compromise macrophage function rendered C57BL mice susceptible to EMC-D-induced diabetes. In macrophage culture for BALB/c mice, EMC-B induced IFN at an earlier stage than did EMC-D. The C57BL mouse-derived macrophages produced more IFN than did BALB/c mouse-derived macrophages after stimulation with EMC-D. Moreover, C. parvum increased IFN production in macrophage cultures from BALB/c mice, whereas carrageenan inhibited that in macrophage cultures from C57BL mice. These results suggest that IFN derived from macrophages may have an important role in protecting mice against EMC virus infection.
Hirasawa
K
K
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Ogiso
Y
Y
Takeda
M
M
Lee
M J
MJ
Itagaki
S
S
Doi
K
K
D000818:Animals; D018188:Cardiovirus Infections / Q000503:physiopathology / Q000517:prevention & control*; D002351:Carrageenan / Q000494:pharmacology; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004686:Encephalomyocarditis virus; D016908:Gram-Positive Bacterial Infections; D066298:In Vitro Techniques; D007372:Interferons / Q000037:antagonists & inhibitors / Q000502:physiology*; D007515:Islets of Langerhans / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011425:Propionibacterium acnes / Q000502:physiology
D002351:Carrageenan; D007372:Interferons