Characterisation of colony-stimulating activity in the avian T cell-derived factor, Salmonella enteritidis-immune lymphokine.
This investigation was designed to characterise the specific cytokine activity from the conditioned medium of concanavalin A-stimulated avian T cells derived from Salmonella enteritidis-immune chickens, S enteritidis-immune lymphokine (ILK). Studies were designed to determine first, whether colony-stimulating activity was present in ILK, second, the type(s) of colonies from the bone marrow that were supported in vitro by the potential colony-stimulating factors in ILK and, third, whether colony-stimulating activity was present in serum from chicks treated with ILK and challenged with S enteritidis, and to use physicochemical treatment as a means of identifying the potential colony-stimulating factor(s) in ILK. Both ILK alone and serum from chicks treated with ILK and challenged with S enteritidis caused significant increases in the number of colony-forming units (CFU) from the bone marrow in vitro. After 10 days of incubation, ILK alone supported the in vitro growth of granulocytic bone marrow colonies. The colony-stimulating activity from serum derived from chicks treated with ILK and challenged with S enteritidis peaked two hours after the challenge. When ILK was either heated at 100 degrees C or treated with trypsin or acid and then injected into chicks, all the chicks responded with significant increases in circulating polymorphonuclear leucocytes (PMNs). However, when assayed for in vitro colony-stimulating activity, only trypsinisation destroyed the activity in ILK. The results indicate that a colony-stimulating factor which preferentially supported the growth of granulocytic bone marrow colonies was present in ILK and that the factor was stable to heat and acid but sensitive to trypsin.
McGruder
E D
ED
Department of Veterinary Pathobiology, Texas A & M University, College Station, USA.
Kogut
M H
MH
Corrier
D E
DE
Deloach
J R
JR
Hargis
B M
BM
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002645:Chickens; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D008222:Lymphokines / Q000302:isolation & purification / Q000494:pharmacology*; D011201:Poultry Diseases; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin
D008222:Lymphokines; D014357:Trypsin