An invertebrate (Molluscan) plasma protein that binds to vertebrate immunoglobulins and its potential for yielding false-positives in antibody-based detection systems.
Commercial immunoassay kits that incorporate monoclonal antibodies are being used increasingly to screen tissues and plasma from invertebrates for the presence of antigens that are taken to be homologues of the vertebrate immunogen. We report the presence of a protein (BgL115r) in the plasma of Biomphalaria glabrata that binds to immunoglobulins from a variety of vertebrates. BgL115r (ca. 230 kD non-reduced, ca. 115 kD reduced on SDS-PAGE) binds to the F(ab')2-region of murine IgG. Binding could be inhibited with high concentrations of N-acetyl-D-glucosamine (GlcNAc). BgL115r was found to yield false positive signals in an ELISA based assay.
Hahn
U K
UK
Department of Zoology, Oregon State University, Corvallis 97331-2914, USA.
Fryer
S E
SE
Bayne
C J
CJ
D000818:Animals; D001700:Biomphalaria / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006458:Hemolymph / Q000276:immunology*; D007136:Immunoglobulins / Q000378:metabolism*; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D029584:Prostatic Secretory Proteins; D011485:Protein Binding; D011933:Reagent Kits, Diagnostic
D007136:Immunoglobulins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011933:Reagent Kits, Diagnostic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors