title: Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA.
abstract: Conversion from the nonmucoid to the mucoid phenotype is a typical feature of Pseudomonas aeruginosa strains causing chronic pulmonary infections in cystic fibrosis patients. One of the key genetic controls in this conversion to mucoidy is from the algT(U)-mucA-mucB(algN) locus, located at 67.5 min on the standard P. aeruginosa chromosomal map. The algT gene promotes conversion to mucoidy and encodes an alternative sigma factor (sigma E) which belongs to the ECF (for extracytoplasmic function) family. On the other hand, the mucA and mucB (algN) genes suppress conversion to mucoidy. Loss-of-function mutations in mucA have been postulated to be the cause of mucoidy in some P. aeruginosa strains isolated from cystic fibrosis patients. We expressed and purified the protein products from the mucA and mucB open reading frames. The purified MucA protein abolishes the in vitro transcription specified by AlgT and the ability of AlgT to compete with an Escherichia coli sigma factor, FliA, suggesting that inhibiting AlgT-dependent transcription could be the mechanism by which mucA suppresses mucoidy in vivo. Enzyme-linked immunosorbent assay and glycerol density gradient sedimentation experiments suggest that MucA physically interacts with AlgT.
authors: Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago 60612, USA.
authors: Z D
authors: ZD
authors: Xie
authors: C D
authors: CD
authors: Hershberger
authors: S
authors: S
authors: Shankar
authors: R W
authors: RW
authors: Ye
authors: A M
authors: AM
authors: Chakrabarty
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003550:Cystic Fibrosis / Q000150:complications; D017931:DNA Primers; D005785:Gene Conversion; D005798:Genes, Bacterial; D006801:Humans; D007700:Kinetics; D008171:Lung Diseases / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000209:etiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012808:Sigma Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013489:Suppression, Genetic
chemical_list: C079619:AlgU protein, Pseudomonas aeruginosa; D001426:Bacterial Proteins; D017931:DNA Primers; C063757:MucA protein, Bacteria; C093590:MucB protein, Bacteria; D011994:Recombinant Proteins; D012808:Sigma Factor