Cultivation of mouse-mouse hybridomas producing IgM monoclonal antibodies.
The mouse-mouse hybridomas producing monoclonal antibodies (MAbs) for ABO blood group determination were cultivated for 10 days in 25 cm2 Roux bottles using standard cultivation medium (DMEM) with different foetal-calf serum (FCS) concentrations (2-13%). The highest specific production rates (200-1100 micrograms/10(6) cells/day) for MAbs were measured at the end of the cultivation: this phenomenon could be explained by advanced cell death and liberating the content of the cells into the medium.
Knezević
M
M
BIA Ltd., Ljubljana, Slovenia.
Alscher
M
M
Zunec
P
P
Hartman-Pretnar
K
K
Rozman
P
P
Curin-Serbec
V
V
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D051379:Mice
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M