A rapid screening procedure to identify mini-Tn10 insertion mutants of Escherichia coli K-12 with altered adhesion properties.
A rapid screening procedure was developed for detection of Escherichia coli mutants with altered adhesion abilities using polystyrene 96-well microtiter plates as attachment surfaces. During this assay, bacterial strains grew and adhered simultaneously, and attached cells were measured after crystal violet staining. Starting with a total of 7000 W3110::Tn10 insertion mutants of E. coli K-12 W3110, 50 adhesion-deficient mutants were isolated which showed less than 40% attachment, and 22 mutants were found with an attachment of 40-75%. Motility assays were performed on these 72 mutants, and 34 displayed altered motility.
Genevaux
P
P
Laboratoire de Microbiologie, Centre des Sciences de l'Environnement, Metz, France.
Muller
S
S
Bauda
P
P
D001422:Bacterial Adhesion / Q000235:genetics*; D001431:Bacteriological Techniques; D018441:Biofilms; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005069:Evaluation Studies as Topic; D009154:Mutation
D004251:DNA Transposable Elements