title: Cultured leptomeningeal cells secrete cerebrospinal fluid proteins.
abstract: To extrapolate the function of the leptomeninges, we examined the profile of the proteins secreted from the cultured leptomeningeal cells prepared from 1-2-day-old rats. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the medium conditioned with the cultured cells, 20-25 differentially distinctive protein bands were noted. Through several chromatographic procedures (Sephadex G-75, Mono Q, and 7C8-300), altogether 18 proteins were purified to homogeneity, and the partial amino acid sequence of each protein was determined. Homology search revealed that the major proteins included prostaglandin-D-synthase or beta-trace protein, insulin-like growth factor (IGF)-II, IGF-binding protein-2, apolipoprotein E, beta 2-microglobulin, cystatin C, transferrin, peptidyl-prolyl cis-trans isomerase or cyclophilin C, secreted protein acidic and rich in cysteine, ubiquitin, lysozyme C, extracellular superoxide dismutase, and collagen alpha-1 (III). Most of these proteins are known to be the major brain-derived protein constituents of CSF and are thought to play important roles in certain biological events in the brain. Considering the morphological features, the present findings suggest the importance of the leptomeninges as an origin of such proteins in CSF.
authors: Department of Molecular Physiology, Gunma University, Maebashi, Japan.
authors: Y
authors: Y
authors: Ohe
authors: K
authors: K
authors: Ishikawa
authors: Z
authors: Z
authors: Itoh
authors: K
authors: K
authors: Tatemoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D001099:Arachnoid / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002556:Cerebrospinal Fluid Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002845:Chromatography; D017077:Culture Media, Conditioned; D055316:Cystatin C; D015891:Cystatins / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D007335:Insulin-Like Growth Factor II / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D008578:Meninges / Q000166:cytology* / Q000378:metabolism; D008969:Molecular Sequence Data; D015676:Osteonectin / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D010841:Pia Mater / Q000166:cytology / Q000378:metabolism; D051381:Rats; D014168:Transferrin / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D002556:Cerebrospinal Fluid Proteins; C525032:Cst3 protein, rat; D017077:Culture Media, Conditioned; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D003911:Dextrans; D005782:Gels; D015676:Osteonectin; D014168:Transferrin; D001613:beta 2-Microglobulin; D007335:Insulin-Like Growth Factor II; C025614:sephadex