Effect of maternal glucocorticoid treatment on ovine fetal fluids at 0.6 gestation.
This study examined the effects of maternal dexamethasone treatment on the volume and composition of fetal fluids, and on placental morphology at 0.6 gestation (80-90 days). Nine pregnant ewes were infused with dexamethasone (D, 0.76 mg h-1 for 72 h) while an additional nine ewes received saline (S, 0.38 mL h-1 for 72 h). Allantoic fluid (ALF) volume was significantly greater (P < 0.01) in the D group (737 +/- 116 mL) than in the S group (190 +/- 55 mL), but there was no difference in amniotic fluid (AMF) volume. The urine flow rate was 11 times higher in three D fetuses. The 51Cr-EDTA infused into the bladders of four fetuses during the final 4-5 h of the 72 infusions was detected in both AMF and ALF. Dexamethasone treatment significantly altered the composition of the fetal fluids but had no affect on fetal body weight, organ weights and placental weight; however, there were fewer cotyledons under 5 g (P < 0.05). In the D group, 3% of cotyledons were of the 'bovine' type in morphology, whereas all cotyledons in the S group were of the 'ovine' type. These findings suggest that prolonged exposure to large doses of glucocorticoids during pregnancy would affect the volume and composition of the fetal fluids and placental morphology, with potentially detrimental effects on the fetus.
Tangalakis
K
K
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Vic., Australia.
Moritz
K
K
Shandley
L
L
Wintour
E M
EM
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000482:Allantois / Q000378:metabolism; D000653:Amniotic Fluid; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005333:Fetus / Q000187:drug effects / Q000378:metabolism*; D005632:Fructose / Q000097:blood / Q000652:urine; D006854:Hydrocortisone / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008431:Maternal-Fetal Exchange; D009994:Osmolar Concentration; D010920:Placenta / Q000033:anatomy & histology / Q000187:drug effects; D011247:Pregnancy; D012756:Sheep; D014556:Urine
D001786:Blood Glucose; D007773:Lactates; D005632:Fructose; D019344:Lactic Acid; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone