Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.
A 5-h PCR assay (Amplicor enterovirus test) was compared with viral culture for the detection of enteroviruses in cerebrospinal fluid. Of the cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 34% were positive by viral culture whereas 66% were positive by the Amplicor PCR, suggesting that this technique improves the diagnosis of enteroviral meningitis.
Yerly
S
S
Central Laboratory of Virology, Geneva University Hospital, Switzerland.
Gervaix
A
A
Simonet
V
V
Caflisch
M
M
Perrin
L
L
Wunderli
W
W
D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D005069:Evaluation Studies as Topic; D006801:Humans; D008582:Meningitis, Aseptic / Q000453:epidemiology / Q000821:virology*; D008587:Meningitis, Viral / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012367:RNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation
D017931:DNA Primers; D012367:RNA, Viral