Treatment of experimental mouse bladder tumour by LPS-induced epithelial cell shedding.
The purpose of the present study was to explore the therapeutic potential of serial administration of shedding-inducing endotoxin in a mouse tumour bladder model. The studies were conducted with two variants derived from the MBT-2 tumour namely, T5 and T50, the latter being far more aggressive than the former. It was found that T5 tumours responded to intravesical lipopolysaccharides (LPS) instillation by a considerable reduction in their pace of growth (P < 0.0001) when treatment was initiated 3 days after tumour implantation, but not when started after 7 days. The T50 variant did not respond to LPS when treated 3 days after implantation, but a considerable reduction in rate of growth occurred when treatment was started after 1-2 days. Shedding induced by intravesically instilled LPS was found to retard considerably the progression rate of experimental bladder tumour.
Nativ
O
O
Department of Urology, Bnai Zion Medical Center, Haifa, Israel.
Medalia
O
O
Mor
Y
Y
Shajrawi
I
I
Sabo
E
E
Aronson
M
M
D000283:Administration, Intravesical; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D018450:Disease Progression; D004731:Endotoxins / Q000008:administration & dosage / Q000627:therapeutic use; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D008809:Mice, Inbred C3H; D013997:Time Factors; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000628:therapy*
D004731:Endotoxins; D008070:Lipopolysaccharides