Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.
Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.
Klespies
S L
SL
Department of Pathology and Laboratory Medicine, Children's Hospital Medical Center of Akron, Ohio 44308, USA.
Cebula
D E
DE
Kelley
C L
CL
Galehouse
D
D
Maurer
C C
CC
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003951:Diagnostic Errors; D004770:Enterovirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect / Q000706:statistics & numerical data; D006801:Humans; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral