Serum level determination of fluconazole by high-performance liquid chromatography and bioassay.
An HPLC method using a reverse phase system, an isocratic mobile phase and 1-phenyl-1,2 ethanediol as internal standard and a well diffusion bioassay using a strain of Candida pseudotropicalis were compared for the measurement of fluconazole in serum. Both methods permit determination of fluconazole in the range from 1 mg/L to 30 mg/L. The correlation between both methods was found to be r = 0.89. Both methods are useful for monitoring the serum level of fluconazole in clinical routine work.
Hülsewede
J W
JW
Institute of Medical Microbiology, University of Essen, Germany.
Dermoumi
H
H
D000935:Antifungal Agents / Q000097:blood*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D015725:Fluconazole / Q000097:blood*
D000935:Antifungal Agents; D015725:Fluconazole