Role of transforming growth factor-beta in the restitution of injured guinea pig gastric mucosa in vitro.
The role of transforming growth factor-beta (TGF-beta) in restitution was examined in intact sheets of injured guinea pig gastric mucosa in which the epithelial cell-collagen interaction can be quantitatively evaluated. The luminal surface of intact sheets of in vitro guinea pig gastric mucosa was injured by exposure to 1.25 mol/l NaCl for 10 min. Restitution was evaluated by measurement of transmucosal electrical resistance and [3H]mannitol flux before and after injury. Recovery of electrical resistance and [3H]mannitol flux was retarded by inhibition of endogenous TGF-beta with either aprotinin or anti-TGF-beta antibody; effects were restored by human recombinant TGF-beta1. During inhibition of endogenous TGF-beta, type IV collagen accelerated the recovery. Inhibition of reconstruction of the basement membrane by simultaneous addition of cis-4-OH-L-proline and anti-type IV collagen completely abolished the enhancement of the recovery by TGF-beta 1. These results suggest that endogenous TGF-beta is required for restitution to occur in guinea pig gastric mucosa and that type IV collagen plays an important role in TGF-beta-abetted restitution.
Yanaka
A
A
Department of Gastroenterology, University of Tsukuba, Ibaraki, Japan.
Muto
H
H
Fukutomi
H
H
Ito
S
S
Silen
W
W
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003094:Collagen / Q000276:immunology / Q000494:pharmacology; D046508:Culture Techniques; D017097:Electric Impedance; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries* / Q000503:physiopathology; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008353:Mannitol / Q000493:pharmacokinetics; D011994:Recombinant Proteins; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D014945:Wound Healing
D000906:Antibodies; D011994:Recombinant Proteins; D012462:Saline Solution, Hypertonic; D016212:Transforming Growth Factor beta; D008353:Mannitol; D003094:Collagen