title: The majority of autologous cytolytic T-lymphocyte clones derived from peripheral blood lymphocytes of a melanoma patient recognize an antigenic peptide derived from gene Pmel17/gp100.
abstract: Anti-melanoma cytolytic T-lymphocyte (CTL) clones were derived from peripheral blood lymphocytes of HLA-A2 melanoma patient LB265 after stimulation with the autologous tumor cell line LB265-MEL, which showed high expression of melanocyte-lineage specific genes. Of 55 CTL clones, 46 recognized HLA-A2-restricted antigens. These 46 CTL clones were studied for their ability to specifically release tumor necrosis factor in the presence of COS cells cotransfected with the HLA-A2 gene and the cDNA of either tyrosinase, Melan-A/MART1, Pmel17/gpl00, gp75/TRP1, or MSH receptor. Six CTL clones recognized the Melan-A/MART1 antigen, whereas the remaining 40 CTL clones recognized a Pmel17/gp100 antigen. These 40 anti-PmelI7/gpl00 CTL clones were all able to lyse T2 cells pulsed with the antigenic peptide YLEPGPVTA, as previously reported. The T-cell receptor beta chain hypervariable region was sequenced and found to be identical in the 15 CTL clones analyzed. Taken together, these data show a high frequency of Pmell7/gp100-specific T cells in autologous antitumor CTL clones derived from peripheral blood of a melanoma patient.
authors: The Ludwig Institute for Cancer Research, University of Louvain, Brussels, Belgium.
authors: H
authors: H
authors: Zarour
authors: C
authors: C
authors: De Smet
authors: F
authors: F
authors: Lehmann
authors: M
authors: M
authors: Marchand
authors: B
authors: B
authors: Lethé
authors: P
authors: P
authors: Romero
authors: T
authors: T
authors: Boon
authors: J C
authors: JC
authors: Renauld
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D002999:Clone Cells; D005260:Female; D005796:Genes; D015789:HLA-A2 Antigen / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; D015345:Oligonucleotide Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta