title: Changes in action potentials and ion currents in long-term cultured neonatal rat ventricular cells.
abstract: A primary culture of neonatal ventricular myocytes isolated from day-old rats was established for investigating the changes in action potentials and ion currents over long periods. Cells at days 5 and 15 in culture were studied. These changes in vitro were compared with those in situ derived from the age-matched freshly isolated cells. During primary culture, quiescent cells demonstrated shortening of action potential durations (APD) resembling the developmental changes observed in situ. The beating cultured cells were not associated with APD shortening. Despite constant current amplitudes, the densities of Ca2+ currents (ICa) decreased in the quiescent cultures at later ages as a result of cell enlargement. ICa densities were maintained in the beating cultured and freshly isolated cells. Acceleration in the inactivation of ICa was observed during developments both in vitro and in situ. In addition, the densities of transient outward currents (Ito) tripled and doubled in the quiescent and beating cells during 15-day cultures. However, Ito in beating cultured cells made less contribution to APD in contrast to the quiescent cultured and freshly isolated myocytes. These findings demonstrate that electrophysiological properties differ between two types of long-term cultured cells. ICa densities remained constant in the beating cultures, suggesting that cell beating may be required for the maintenance of ICa density in developing cardiomyocytes.
authors: Department of Circulation, Nagoya University, Japan.
authors: W
authors: W
authors: Guo
authors: K
authors: K
authors: Kamiya
authors: J
authors: J
authors: Cheng
authors: J
authors: J
authors: Toyama
mesh_terms: D000200:Action Potentials; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D004553:Electric Conductivity; D004594:Electrophysiology; D007473:Ion Channels / Q000502:physiology*; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016276:Ventricular Function
chemical_list: D015220:Calcium Channels; D007473:Ion Channels