Cholesterol-containing lactose derived neoglycolipids serve as acceptors for sialyltransferases from rat liver Golgi vesicles.
The cholesterol-containing lactose derived neoglycolipids beta-Lactosylcholesterol, Cholesteryl-beta-lactosylpropane-1,3-diol, 3-Cholesteryl-1-beta-lactosylglycerol, 2-Cholesteryl-1-beta-lactosylglycerol, 2,3-Dicholesteryl-1-beta-lactosylglycerol, 1-Deoxy-1-cholesterylethanolaminolactitol, 1-Deoxy-1-cholesteryl (N-acetyl)-ethanolaminolactitol, 1-Deoxy-1-cholesterylphosphoethanolaminolactitol, and 1-Deoxy-1-cholesterylphospho (N-acetyl)-ethanolaminolactitol were synthesized and used as acceptors for sialytransferases from rat liver to Golgi vesicles. Relative activities with the neoglycolipids as acceptors varied from 28 to 163% compared to those obtained with the authentic acceptor lactosylceramide. Product identification by thin layer chromatography and fast atom bombardment mass spectrometry showed that the neoglycolipids yielded mono- and disialylated products. The results of competition experiments suggested that lactosylceramide and the neoglycolipids were sialylated by the same enzymes.
Pohlentz
G
G
Physiologisch Chemisches Institut der Universität Bonn, FRG.
Mokros
A
A
Egge
H
H
D000818:Animals; D002784:Cholesterol; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology*; D007700:Kinetics; D007785:Lactose; D008099:Liver / Q000201:enzymology*; D051381:Rats; D012794:Sialic Acids; D012799:Sialyltransferases / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013379:Substrate Specificity
D006017:Glycolipids; D012794:Sialic Acids; D002784:Cholesterol; D012799:Sialyltransferases; D007785:Lactose