title: A transgenic mouse line with alpha-1,3/4-fucosyl-transferase cDNA: production and characteristics.
abstract: cDNA of human alpha-1,3/4-fucosyltransferase (Fuc-TIII) was placed under the control of the chicken beta-actin promoter and cytomegalovirus enhancer, then introduced into male pronuclei of fertilized mouse eggs. A transgenic mouse line thus obtained exhibited enhanced expression of Lex (4C9) antigen in endothelial cells located in the glomerulus, sinusoidal capillaries of the liver and capillaries of the heart. Furthermore, in the transgenic mice, sialyl dimeric Lex (FH6) and sialyl Lea (2D3) antigens were strongly expressed in the glomerular endothelial cells.
authors: Department of Biochemistry, Kagoshima University, Japan.
authors: H
authors: H
authors: Obama
authors: T
authors: T
authors: Kaname
authors: A
authors: A
authors: Sudou
authors: T
authors: T
authors: Yanagida
authors: S
authors: S
authors: Ikematsu
authors: M
authors: M
authors: Ozawa
authors: H
authors: H
authors: Yoshida
authors: R
authors: R
authors: Kannagi
authors: K I
authors: KI
authors: Yamamura
authors: T
authors: T
authors: Muramatsu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002236:Carbohydrate Conformation; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D005647:Fucosyltransferases / Q000235:genetics*; D007668:Kidney / Q000276:immunology; D016256:Lewis X Antigen / Q000235:genetics*; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009206:Myocardium / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D016256:Lewis X Antigen; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase