Induction of T cell responses to the invariant chain derived peptide CLIP in mice immunized with the group 1 allergen of house dust mite.
In this study we demonstrate that immunization of H-2(b) mice with the allergen Der p 1 induces MHC class II restricted T cells that proliferate to residues 15-29 of Der p 1 (p15-29) and to the murine MHC class II-associated invariant chain derived peptide (CLIP). T cells from naive H-2(b) mice and those immunized with murine CLIP fail to respond to either CLIP or p15-29. T cell lines and clones reactive with p15-29 strongly proliferate in response to splenic antigen-presenting cells (APC) from normal H-2(b) mice but show reduced proliferation to APC from invariant chain deficient mice. Furthermore, T cells isolated from Der p 1 primed mice and expanded on H-2(b) spleen cells in the absence of the p15-29 epitope retained specificity for both p15-29 and CLIP, suggesting that naturally presented self components can act as mimetic peptides and may maintain T cell memory to foreign antigens.
Kristensen
N M
NM
Infection and Immunity Section, Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK.
Hoyne
G F
GF
Hayball
J D
JD
Hetzel
C
C
Bourne
T
T
Lamb
J R
JR
D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination
D000485:Allergens; D015703:Antigens, CD; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte; D006023:Glycoproteins; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C045084:invariant chain