Pathogenesis of Chlamydia psittaci infection in sheep: detection of the organism in a serial study of the lymph node.
Sixteen sheep were inoculated subcutaneously over the left prefemoral lymph node with an abortifacient strain of Chlamydia psittaci. Groups of four animals were killed after 3, 6, 12 and 18 days. Four of eight sheep which received a control inoculum were killed on day 6 and four on day 18. The left and right prefemoral lymph nodes were removed and weighed and portions taken from each for examination by the polymerase chain reaction (PCR), by culture, and by histopathological and immunohistochemical methods. The left prefemoral lymph nodes enlarged after the injection of C. psittaci, with the group mean weight on day 6 being the greatest and that on day 18 being normal. Examination by "nested" PCR showed samples from these nodes to be positive, except for one animal killed on day 3 and one on day 12. Live organisms, however, were not cultured from any of the samples collected. C. psittaci antigen was detected immunohistochemically in three of four nodes on day 3, in each of four on day 6, and in two of four on both days 12 and 18. Nodes from the contralateral side remained normal, as did those from unchallenged control sheep, and no antigen or DNA was detected in them.
Buxton
D
D
Moredun Research Institute, Edinburgh, UK.
Rae
A G
AG
Maley
S W
SW
Thomson
K M
KM
Livingstone
M
M
Jones
G E
GE
Herring
A J
AJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001483:Base Sequence; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis*; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D009956:Psittacosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000473:pathology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D008070:Lipopolysaccharides