Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa.
Flagellin gene sequences from 64 clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa were amplified by PCR and subjected to RFLP analysis by using seven restriction enzymes to digest the amplified products. Using this approach the isolates were assigned to one of 13 groups. The method was rapid, reproducible and applicable to all isolates. In contrast, serotyping failed to satisfactorily resolve 49% of the strains tested. The vast majority of clinical isolates generated amplified products of 1.02 kb (type a) or 1.25 kb (type b). Electron microscopical analysis revealed evidence fax some. flagellar structural variation between P. aeruginosa strains. This study provides further evidence that the flagellin gene is a widely applicable and useful genetic marker for studying genetic variation within populations of closely related bacteria.
Winstanley
C
C
School of Natural and Environmental Sciences, Coventry University, UK. apx086@coventry.ac.uk
Coulson
M A
MA
Wepner
B
B
Morgan
J A
JA
Hart
C A
CA
D001483:Base Sequence; D017931:DNA Primers; D005407:Flagella / Q000648:ultrastructure; D005408:Flagellin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping; D012703:Serotyping
D017931:DNA Primers; D005408:Flagellin