[The purification and analysis of S-antigen in rabbit retina].
OBJECTIVE
S-antigen from rabbit retina was isolated and purified to investigate the immune mechanisms of experimental autoimmune uveoretinitis (EAU) and lay a foundation for its experimental treatment.


METHODS
Two-step ion exchange chromatography was used for the purification of S-antigen and it was analyzed.


RESULTS
The analytical studies show that the molecular weight of the purified S-antigen is 55,000, its isoelectric point is 6.40, and non-polar amino acid constitutes a relatively large proportion in its amino acids. Scanning tunnel electron microscope was used to investigate the configuration of S-antigen. It is elliptic globe-like. The purified S-antigen successfully induced the model of EAU.


CONCLUSIONS
S-antigen purified by two-step ion exchange chromatography can be used for its own analytical studies and induction of EAU model.
Sun
X
X
Beijing Institute of Ophthalmology.
Zhang
C
C
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000302:isolation & purification*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000209:etiology; D002852:Chromatography, Ion Exchange; D005136:Eye Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000737:chemistry*; D012173:Retinitis / Q000209:etiology; D014605:Uveitis / Q000209:etiology
D000596:Amino Acids; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins