Endothelin-1-induced incorporation of cholesterol into rat adrenals.
The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quarters in vitro; coinfusion of ET-1 with tritiated cholesterol-enriched serum and determination of adrenal-associated radioactivity; and tritiated cholesterol incorporation in incubations of adrenal cells. In all cases ET-1 increased cholesterol uptake. Subcellular fractionation showed an ET-1-mediated augmentation in mitochondrial fraction. This increase was mediated by the subpopulation B of adrenal receptors for ET-1. In addition, ET-1 also increased cytochrome P450-SCC (side-chain cleavage) activity.
Romero
D G
DG
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Pecci
A
A
Lantos
C P
CP
Cozza
E N
EN
D000311:Adrenal Glands / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000450:Aldosterone / Q000096:biosynthesis; D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D019332:Endothelin-1 / Q000494:pharmacology*; D007262:Infusions, Intravenous; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D019332:Endothelin-1; D000616:Aminoglutethimide; D000450:Aldosterone; D002784:Cholesterol