Cloning and expression of Kluyveromyces fragilis LAC4 gene.
The genomic library of Kluyveromyces fragilis was constructed in E. coli TG1, and 5 beta-galactosidase gene (LAC4) clones have been obtained from the library by complementation of the Kluyveromyces lactis lac4-8 mutation. The studies on the structure and the function of the LAC4 gene revealed that (i) the gene can also complement E. coli lacZ mutation; (ii) the physical map of the K. fragilis LAC4 gene was very similar to that of K. lactis; (iii) the beta-galactosidase levels expressed by the clone strains were much higher than that expressed by the original strain; (iv) the variation of the beta-galactosidase level of different clone strains induced by lactose or galactose was related to the retained degree of the 5' flanking region of LAC4 gene, suggesting that there might be a lactose specific transcription activating element in the region.
Huo
K
K
Institute of Genetics, Fudan University, Shanghai, China.
Li
Y
Y
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D015698:Genomic Library; D007716:Kluyveromyces / Q000235:genetics*; D007785:Lactose / Q000494:pharmacology; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D001616:beta-Galactosidase; D007785:Lactose; D005690:Galactose