[The inhibitory effects of serum of patients with acute promyelocytic leukemia on the formation of megakaryocytopoiesis colony unit].
OBJECTIVE
To determine if the sera of patients with acute promyelocytic leukemia (APL) and all-trans retinoic acid (ATRA) inhibit the formation of colony-forming-unit of megakaryocyte (CFU-Meg).


METHODS
By cellular culture assay, the sera of 22 APL patients treated with ATRA and 10(-6)' mol/L ATRA were kinetically used to perform the investigation. Meanwhile, serum tumor necrosis factor (TNF) activity and bone marrow CFU-L were also detected.


RESULTS
Before treatment, there was an inhibitory activity on CFU-Meg in the serum of APL patients (29.6% +/- 2.4%). The activity reduced gradually with the treatment and was stimulus when bone marrow remission obtained (-18.7% +/- 4.1%, P < 0.001). It was positively correlated with TNF activity in the serum and the number of CFU-L however negatively correlated with the number of megakaryocyte in bone marrow and PLT. Serum TNF activity and the number of CFU-L before treatment were higher than those after treatment (P < 0.05 and < 0.001, respectively). ATRA could stimulate the growth of CFU-Meg.


CONCLUSIONS
There was an inhibitory activity in the serum of APL patients which could be used to monitor the efficacy of the therapy. ATRA stimulates the growth of CFU-Meg.
Zhou
R
R
Shanghai Ruijing Hospital, Shanghai Institute of Hematology.
Sun
G
G
Wu
W
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood* / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000473:pathology*; D008875:Middle Aged; D010976:Platelet Count; D014212:Tretinoin / Q000494:pharmacology; D014410:Tumor Stem Cell Assay
D014212:Tretinoin