Genetic identification of cryptic genospecies of Haemophilus causing urogenital and neonatal infections by PCR using specific primers targeting genes coding for 16S rRNA.
Previous genetic analysis of Haemophilus influenzae strains isolated from genital and neonatal infections identified a group of biotype IV that constitutes a cryptic genospecies only distantly related to H. influenzae and H. Haemolyticus. Small-subunit rRNA genes of two representative strains of this genital Haemophilus genospecies (strains 16N and 2406) were sequenced. The analysis indicated that these strains form a monophyletic unit with H. haemolyticus and H. influenzae biogroups Influenzae and Aegyptius and are more closely related to H. haemolyticus than to H. influenzae biogroups Influenzae and Aegyptius. 16S rRNA gene sequences were used to formulate primers for PCR-based identification of cryptic genital Haemophilus organisms. A 242-bp fragment was amplified from strains belonging to the genital Haemophilus genospecies but not from strains of 12 other Haemophilus species, including strains of H. influenzae biotype IV sensu stricto.
Quentin
R
R
Département de Microbiologie Médicale et Moléculaire, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1334, CHU Bretonneau, Tours, France.
Ruimy
R
R
Rosenau
A
A
Musser
J M
JM
Christen
R
R
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D052776:Female Urogenital Diseases / Q000209:etiology* / Q000382:microbiology; D005798:Genes, Bacterial; D006190:Haemophilus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D006192:Haemophilus Infections / Q000209:etiology* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D007231:Infant, Newborn; D052801:Male Urogenital Diseases; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S