Expression and localization of alpha v integrins and their ligand vitronectin in normal ovarian epithelium and in ovarian carcinoma.
In an extension of a previous in vitro study [Carreiras et al., Int. J. Cancer 63, 530-536 (1995)] and in an effort to understand the adhesive interactions mediated by integrins within epithelial ovarian tumors, the presence of the alpha v and beta 3 subunits and that of vitronectin (Vn) in ovarian carcinomas at various stages of differentiation and in normal ovarian epithelium were comparatively investigated. The study was performed on material from 34 patients. By immunofluorescence, cryostat sections were analyzed for their expression of alpha v (34 cases), beta 3 (19 cases), and Vn (29 cases). alpha v was expressed in normal epithelium and in highly differentiated tumors as well as in a majority of moderately and poorly differentiated carcinomas with identical staining pattern. beta 3 subunit and Vn were also expressed in normal cases and highly differentiated carcinomas. However, they were lacking in most of the less differentiated tumors. The analysis of cases which were simultaneously tested for the presence of alpha v, beta 3, and Vn revealed that a large proportion of normal ovarian epithelium and highly differentiated tumors simultaneously expressed alpha v, beta 3, and Vn; in contrast, in all moderately and poorly differentiated carcinomas either beta 3 or Vn was absent. The potential role of the alpha v beta 3/Vn system in ovarian epithelium functions is discussed. It is also speculated that modifications of this system in ovarian carcinomas might contribute to tumor progression.
Carreiras
F
F
Laboratoire de Cancérologie Expérimentale, Centre F. Baclesse, Caen, France.
Denoux
Y
Y
Staedel
C
C
Lehmann
M
M
Sichel
F
F
Gauduchon
P
P
D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D004311:Double-Blind Method; D004848:Epithelium / Q000737:chemistry; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039564:Integrin alphaV; D039661:Integrin beta3; D016023:Integrins / Q000032:analysis / Q000096:biosynthesis*; D010051:Ovarian Neoplasms / Q000737:chemistry*; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D019096:Vitronectin / Q000032:analysis / Q000096:biosynthesis*
D015703:Antigens, CD; D039564:Integrin alphaV; D039661:Integrin beta3; D016023:Integrins; D010980:Platelet Membrane Glycoproteins; D019096:Vitronectin