Heterogeneity of breakpoints at the transcriptional co-activator gene, BOB-1, in lymphoproliferative disease.
Chromosome 11q23 is frequently a site of chromosomal translocation in both acute leukemias and chronic lymphoproliferative disorders. In the former, an 8 kb region within the MLL gene is consistently involved, whereas in the latter breakpoints appear to be heterogeneous. In a B cell acute leukemia cell line with t(14;18)(q32.3;q21.3) we have previously demonstrated a reciprocal translocation between the LAZ3/BCL6 gene at 3q27 and the B cell specific transcriptional coactivator gene BOB-1 at 11q23.1, implicating BOB-1 as a potential proto-oncogene. To confirm the chromosomal localization of BOB-1 we have mapped it by FISH to 11q23.1. It lay immediately telomeric of the ATM gene. We have also investigated the frequency of BOB-1 rearrangements in a panel of 32 cell lines and 71 patient samples. In one case of T cell prolymphocytic leukemia-a disease where 11q23 abnormalities are observed-a chromosomal rearrangement was identified 3.3-0.9 kb centromeric of the 3' end of the gene. Thus, there is a heterogeneity of breakpoints associated with BOB-1 while the frequency of the gene's involvement in lymphoproliferative diseases is low.
Yuille
M A
MA
Institute of Cancer Research, Sutton, UK.
Galiegue-Zouitina
S
S
Hiorns
L R
LR
Jadayel
D
D
De Schouwer
P J
PJ
Catovsky
D
D
Dyer
M J
MJ
Kerckaert
J P
JP
D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015463:Leukemia, Prolymphocytic / Q000235:genetics; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics; D008232:Lymphoproliferative Disorders / Q000235:genetics*; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D015534:Trans-Activators / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; C091546:POU2AF1 protein, human; D000090063:Proto-Oncogene Mas; D015534:Trans-Activators