Detection of clonal immunoglobulin gene rearrangements in the PBPC harvests of patients with acute lymphoblastic leukaemia.
We have used a fluorescently based PCR technique to detect rearrangements in the immunoglobulin heavy chain (IgH) gene in the presentation BM of five patients with adult ALL and have looked for similar rearrangements in their PBPC. Using this approach we have been able to demonstrate clonal rearrangements in the PBPC of two of five patients. Remission BM samples taken 6-12 weeks prior to leucapheresis failed to show a clonal rearrangement in either patient. The significance of these results is discussed.
Owen
R G
RG
Centre for Haematological Oncology, General Infirmary, Leeds, UK.
Johnson
R J
RJ
Evans
P A
PA
Smith
G M
GM
Child
J A
JA
Morgan
G J
GJ
D001483:Base Sequence; D001853:Bone Marrow / Q000276:immunology* / Q000473:pathology; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D007143:Immunoglobulin Heavy Chains