Replication properties of ARS1 plasmids in Saccharomyces cerevisiae: dependence on the carbon source.
The replication behaviour of a number of ARS1-based plasmids was investigated on propagation in Saccharomyces cerevisiae grown with either glucose or galactose as carbon source. Growth on galactose results in reduced plasmid stability, as well as in reduced replication efficiency, when the entire 1.5-kb TRP1-ARS1 fragment is present on a plasmid. The galactose sensitivity is mediated by a 0.13-kb fragment harbouring part of the GAL3 promoter. This fragment exerts its effect when situated either 5' or 3' to the ARS core consensus at distances up to 0.9 kb. The endogenous 2 microns plasmid remained unaffected by the choice of carbon source.
Kirpekar
F
F
Department of Molecular Biology, Odense University, Denmark.
Gulløv
K
K
D019747:Aldose-Ketose Isomerases; D004261:DNA Replication / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005690:Galactose / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006601:Hexoses / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018741:Replication Origin / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
D005656:Fungal Proteins; C099289:Gal3 protein, S cerevisiae; D006601:Hexoses; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae; D005690:Galactose