Detection of hydatid antigen by co-agglutination in fluid samples from hydatid cysts.
The co-agglutination (Co-A) procedure was standardized and evaluated to detect hydatid antigen in fluid samples aspirated from hydatid cysts. Samples were collected from 14 hydatid cysts in different organs (liver, 7; lung, 5; multiple abdominal cysts, 1; and kidney, 1) by surgery. Echinococcus granulosus scolices and hooklets were seen in only 6 (42.85%) samples by routine microscopy. In contrast, hydatid antigen was demonstrated in all the samples by Co-A (100% sensitivity). Co-A could be employed as a simple and rapid diagnostic procedure, as an alternative to microscopy, to confirm the hydatid aetiology of a suspected cyst.
Parija
S C
SC
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
Ravinder
P T
PT
Shariff
M
M
D000372:Agglutination Tests; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D006801:Humans
D000947:Antigens, Helminth