Micellar electrokinetic capillary chromatographic separation of steroids in urine by trioctylphosphine oxide and cationic surfactant.
Separation of the six structurally similar and hydrophobic neutral steroids, testosterone, dimethyltestosterone, testosterone propionate, cortisone, hydrocortisone and 17-deoxycorticosterone, was achieved by hydrophobic micellar electrokinetic chromatography. A triphasic separation involving micellar dodecyltrimethylammonium bromide (DTAB), a dynamic bilayer formed due to electrostatic interaction between the silica surface and DTAB, and aqueous phase is proposed to account for the observed separation of the steroids. The running buffer consisted of 0.05 M DTAB and 0.0052 M trioctylphosphine oxide in 0.01 M of phosphate buffer pH 7.4. A detection limit of 500 ng/ml was achieved for each steroid and the application of the method to urine samples is described.
Abubaker
M A
MA
University of Texas Medical Branch, Department of Pathology/Laboratory of Medicine. Galveston 77555-0742, USA.
Petersen
J R
JR
Bissell
M G
MG
D002845:Chromatography / Q000379:methods*; D003900:Desoxycorticosterone / Q000652:urine; D019075:Electrophoresis, Capillary; D006801:Humans; D006889:Hydroxycorticosteroids / Q000652:urine; D008823:Micelles; D009943:Organophosphorus Compounds; D000644:Quaternary Ammonium Compounds; D013256:Steroids / Q000652:urine*; D013501:Surface-Active Agents; D013739:Testosterone / Q000031:analogs & derivatives / Q000652:urine
D006889:Hydroxycorticosteroids; D008823:Micelles; D009943:Organophosphorus Compounds; D000644:Quaternary Ammonium Compounds; D013256:Steroids; D013501:Surface-Active Agents; C013912:decyltrimethylammonium; D013739:Testosterone; D003900:Desoxycorticosterone; C044965:trioctyl phosphine oxide