title: Prognostic significance of nuclear DNA content in human meningiomas: a prospective study.
abstract: Flow cytometric DNA analyses were performed to study the correlation between alterations of nuclear DNA content and clinical aggressive tumour behaviour in 134 cranial meningiomas. Forty-one meningiomas revealed an aneuploid DNA content with a distribution of n = 24 in benign, n = 12 in atypical and n = 5 in anaplastic tumours. Aneuploid DNA content was correlated with a significantly higher amount of histomorphological criteria like evidence of mitoses, necrosis, infiltration and increased cellularity. There was a significantly higher Ki 67 proliferation index in the aneuploid meningiomas in comparison to the diploid tumour group. The rate of aneuploid cell-lines was increased in recurrent tumours. No tumour recurrence could be found in diploid meningiomas during follow up (mean 37 months, range 22-46 months). However eight of forty-one aneuploid tumours showed meningioma recurrence. Nuclear DNA content has an important significance in predicting risk of recurrence and poor clinical outcome after benign meningioma surgery.
authors: Neurochirurgische Klinik, Universität Würzburg, Federal Republic of Germany.
authors: J
authors: J
authors: Meixensberger
authors: M
authors: M
authors: Janka
authors: A
authors: A
authors: Zellner
authors: W
authors: W
authors: Roggendorf
authors: K
authors: K
authors: Roosen
mesh_terms: D000782:Aneuploidy; D001921:Brain / Q000473:pathology; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008577:Meningeal Neoplasms / Q000473:pathology* / Q000601:surgery; D008579:Meningioma / Q000473:pathology* / Q000601:surgery; D009336:Necrosis; D009364:Neoplasm Recurrence, Local / Q000473:pathology* / Q000601:surgery; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D004273:DNA, Neoplasm; D019394:Ki-67 Antigen