Protein kinase C inhibitor, chelerythrine, potentiates the adrenaline-mediated aggregation of human platelets through calcium influx.
The role of protein kinase C (PKC) using its selective inhibitor, chelerythrine, in agonist mediated platelet aggregation was studied. Chelerythrine had no effect on the aggregation induced by adrenaline, PAF, collagen and ADP at the maximum doses of these agonists. However, it potentiated the aggregatory response of low doses of adrenaline (0.4-1 microM). Such an effect was blocked by Ca(++)-channel blockers, verapamil and diltiazem indicating the likely involvement of Ca++ influx in the platelet aggregation during the cascade.
Shah
B H
BH
Department of Physiology and Pharmacology, Aga Khan University, Karachi, Pakistan.
Shamim
G
G
Khan
S
S
Saeed
S A
SA
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000470:Alkaloids; D053119:Benzophenanthridines; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D010617:Phenanthridines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*
D000316:Adrenergic alpha-Agonists; D000470:Alkaloids; D053119:Benzophenanthridines; D002121:Calcium Channel Blockers; D004791:Enzyme Inhibitors; D010617:Phenanthridines; D010975:Platelet Aggregation Inhibitors; C016299:chelerythrine; D011493:Protein Kinase C; D002118:Calcium; D004837:Epinephrine