Presence of prostaglandin EP4 receptor gene expression in a rat gastric mucosal cell line.
RGM-1 is an epithelial cell line established from gastric mucosa of adult Wistar rats. In this study, we characterized this newly established cell line by Northern blot analysis. We also investigated deoxyribonucleic acid (DNA) synthesis and hexosamine production in RGM-1 by PGE2. Northern blot analysis did not detect any transcript of proton pump, gastrin receptor, histidine decarboxylase, somatostatin and pepsinogen 1, indicating the absence of characteristics of parietal, ECL, D and chief cells in RGM-1 cells. However, this periodic acid-Schiff (PAS)-positive cell line expressed prostaglandin EP4 receptor mRNA but not EP1 and EP3 receptor mRNAs. [3H]-thymidine incorporation into DNA of the cells was not increased by PGE2. In contrast, PGE2 increased hexosamine content in RGM-1 cells. These results suggest that RGM-1 may be a useful model of gastric mucosal cells and that PGE2 plays a role on mucin synthesis in RGM-1 cells possibly via EP4 receptors.
Hassan
S
S
Department of Medicine, Kobe University School of Medicine, Japan.
Kinoshita
Y
Y
Min
D
D
Nakata
H
H
Kishi
K
K
Matsushima
Y
Y
Asahara
M
M
Wang
H Y
HY
Okada
A
A
Maekawa
T
T
Matsui
H
H
Chiba
T
T
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006595:Hexosamines / Q000096:biosynthesis; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018078:Receptors, Prostaglandin E / Q000096:biosynthesis* / Q000235:genetics
D006595:Hexosamines; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D004247:DNA; D015232:Dinoprostone