Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a-b+) p phenotype: absence of blood group structures based on the globoseries.
Total neutral glycolipid fractions were isolated from kidney and ureter tissue obtained at autopsy of an individual of the rare blood group A1 Le(a-b+) p. The amount of glycolipids isolated were 3.7 and 2.5 mg g-1 dry tissue weight for the kidney and ureter tissue, which is in the range of reference blood group P kidneys. Part of the kidney glycolipid fraction was subfractionated by HPLC. Glycolipid compounds were structurally characterized by thin-layer chromatography (chemical detection and immunostaining with monoclonal antibodies), proton NMR spectroscopy and mass spectrometry. Globotriaosyl- and globotetraosyl-ceramides, which are the major compounds in kidneys of P individuals, were absent in the p kidney, and a comparatively increased amount of monoglycosyl- and lactosylceramides was found. A shift to longer fatty acyl chains in the ceramide part of lactosylceramides was noted. Elongated globoseries compounds with five to seven sugar residues, including the blood group A type 4 chain structure, were lacking. A slight increase in neolactotetraosyl- and blood group X pentaglycosyl-ceramides was noticed. The study confirms an enzymatic block in the conversion of lactosylceramide to elongated globoseries compounds in the kidney tissue similar to that of erythrocytes of p individuals.
Lindström
K
K
Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska Hospital, Sweden.
Jovall
P A
PA
Ghardashkani
S
S
Samuelsson
B E
BE
Breimer
M E
ME
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001789:Blood Group Antigens / Q000096:biosynthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D005915:Globosides / Q000096:biosynthesis* / Q000737:chemistry; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D010127:P Blood-Group System / Q000235:genetics*; D010641:Phenotype; D014513:Ureter / Q000737:chemistry / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D005915:Globosides; D006017:Glycolipids; D006028:Glycosphingolipids; D010127:P Blood-Group System