The rps4-gene is encoded upstream of the nad2-gene in Arabidopsis mitochondria.
In Arabidopsis mitochondria the nad2-gene consists of five exons (a-e) which are separated by three cis-splicing introns and one trans-splicing intron. Sequence analysis of the region upstream of exons a and b reveals an open reading frame encoding ribosomal protein S4 (rps4). In the second nad2 coding region (exons c-e) a pseudo tRNA(Tyr) sequence and a fragment of the plastid psbA gene are located upstream of the trans-spliced exon c. Primer extension analysis identifies RNA 5'-termini within the pseudo-tRNA(Tyr) confirming this sequence to be non-functional. Northern blot analysis suggests the rps4-gene to be cotranscribed with at least the first part of the nad2-gene. The rps4 and nad2 coding sequences as well as the first cis-intron and the trans-intron sequences of the nad2 gene are altered by RNA editing. RNA editing in the open reading frames improves in most instances conservation of the specified amino acids.
Lippok
B
B
Institut für Genbiologische Forschung, Berlin, Germany.
Brennicke
A
A
Unseld
M
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D017393:RNA Editing / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics
D004272:DNA, Mitochondrial; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012365:RNA, Transfer, Tyr; D012269:Ribosomal Proteins; C031798:ribosomal protein S4