title: Isolation of porcine respiratory coronavirus from pigs affected with porcine reproductive and respiratory syndrome.
abstract: Four cytopathogenic viruses were isolated in CPK cells derived from porcine kidneys from tonsils and lungs of 3 of 15 pigs affected with porcine reproductive and respiratory syndrome virus. Physicochemically and morphologically, the isolates were similar to a coronavirus. The isolates were not distinguished from transmissible gastroenteritis virus (TGEV) by a neutralization test using polyclonal antibodies, but differentiated from TGEV by monoclonal antibodies capable of discriminating between TGEV and porcine respiratory coronavirus (PRCV), indicating that the isolates were PRCV. In a serological survey of 30 serum samples each collected from about 50 days old pigs in the 2 affected farms, 29 (97%) and 15 (50%) sera were positive for neutralizing antibody against the isolate with the titers ranging from 2 to 64, respectively.
authors: Ibaraki Kenhoku Livestock Hygiene Service Center, Japan.
authors: O
authors: O
authors: Kamogawa
authors: Y
authors: Y
authors: Tomita
authors: M
authors: M
authors: Kaneko
authors: S
authors: S
authors: Yamada
authors: M
authors: M
authors: Kubo
authors: M
authors: M
authors: Shimizu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D018018:Arterivirus / Q000145:classification / Q000302:isolation & purification*; D018174:Arterivirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000821:virology; D008168:Lung / Q000821:virology*; D008854:Microscopy, Electron; D014066:Palatine Tonsil / Q000821:virology*; D012140:Respiratory Tract Diseases / Q000473:pathology / Q000662:veterinary / Q000821:virology; D013552:Swine; D013553:Swine Diseases; D013577:Syndrome; D005760:Transmissible gastroenteritis virus / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal