Anorectic and neurochemical effects of pituitary adenylate cyclase activating polypeptide in rats.
Pretreatment of rats with intrahypothalamic injections of pituitary adenylate cyclase activating peptide (PACAP) 10 min prior to the injection of neuropeptide Y (NPY) significantly reduced food and water intake during the 4-h measurement period. Intrahypothalamic injection of PACAP in schedule-fed rats also reduced food and water intake for 2 h. A smaller 1-h reduction of water intake was observed in water-deprived rats, suggesting that the anticonsummatory effects of PACAP were primarily against food intake. PACAP treatment did not alter hypothalamic concentration of NPY, nor were neurotransmitters, precursors, or metabolites altered substantially in corpus striatum or nucleus accumbens regions. These results demonstrate primary anorectic effects of intrahypothalamic injection of PACAP. The demonstration of these anorectic effects may suggest a role of cyclic AMP activation and inhibition in the control of satiety and hunger.
Chance
W T
WT
Department of Surgery, University of Cincinnati Medical Center, OH 45267-0558, USA.
Thompson
H
H
Thomas
I
I
Fischer
J E
JE
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000855:Anorexia / Q000139:chemically induced* / Q000503:physiopathology; D000242:Cyclic AMP / Q000378:metabolism; D004326:Drinking / Q000187:drug effects / Q000502:physiology; D004435:Eating / Q000187:drug effects / Q000502:physiology; D006815:Hunger / Q000187:drug effects / Q000502:physiology; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology / Q000502:physiology; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017207:Rats, Sprague-Dawley; D012527:Satiation / Q000187:drug effects / Q000502:physiology; D015290:Second Messenger Systems
C495403:Adcyap1 protein, rat; D009478:Neuropeptide Y; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D000242:Cyclic AMP; D000262:Adenylyl Cyclases