[The effect of adriamycin and ethidium bromide on the Ca2(+)-dependent K channel of human erythrocytes].
The influence of cytotoxic agents adriamycine (AD) and ethydium bromide (EB) on Ca(2+)-dependent K+ channels of erythrocytes was investigated. The incubation of erythrocytes with agliconic part of AD (without aminosugar residue) increased Ca(2+)-dependent K+ efflux induced by low concentration of propranolol, while EB suppressed the activating effect of propranolol. EB, verapamil and triphluoroperazine inhibited Ca(2+)-dependent K+ efflux induced by high doses of propranolol. The incubation of erythrocytes with AD took off the inhibitory action of EB and verapamil but did not influence the blocking effect of triphluoroperazine. Both AD and EB did not influence Ca(2+)-dependent K+ channels induced by Ca(2+)-ionophore A23187, and Pb(2+)-dependent K+ efflux from erythrocytes. It is suggested that opposite effects of AD and EB on Ca(2+)-dependent K+ channels are due to activation by AD and inhibition by EB of system of Ca2+ transport into cells, but not on their action directly on K+ channel.
Davtian
T K
TK
Giul'khandanian
A V
AV
Gambarov
S S
SS
Nagapetian
K G
KG
Aleksanian
Iu T
IuT
Ignatova
T N
TN
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004347:Drug Interactions; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004996:Ethidium / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D011188:Potassium / Q000097:blood; D015221:Potassium Channels / Q000187:drug effects*; D011433:Propranolol / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D002121:Calcium Channel Blockers; D007476:Ionophores; D015221:Potassium Channels; D014268:Trifluoperazine; D000001:Calcimycin; D004317:Doxorubicin; D011433:Propranolol; D014700:Verapamil; D004996:Ethidium; D011188:Potassium; D002118:Calcium
Vliianie adriamitsina i bromistogo étidiia na Ca2(+)-zavisimyĭ K-kanal éritrotsitov cheloveka.