Effects of clofibrate on flow cytometric populations in rat liver.
A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for beta-oxidation, delta 3, delta 2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of beta-oxidation enzyme(s) of the mitochondria in rat liver.
Tomioka
Y
Y
Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi, Japan.
Suzuki
H
H
Miura
K
K
Yamaguchi
T
T
Hishinuma
T
T
Mizugaki
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D019748:Carbon-Carbon Double Bond Isomerases; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002994:Clofibrate / Q000494:pharmacology*; D064006:Dodecenoyl-CoA Isomerase; D005434:Flow Cytometry; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D015151:Immunoblotting; D007535:Isomerases / Q000096:biosynthesis; D008099:Liver / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123; D012235:Rhodamines; D013347:Subcellular Fractions
D000911:Antibodies, Monoclonal; D000960:Hypolipidemic Agents; D012235:Rhodamines; D020112:Rhodamine 123; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase; D002994:Clofibrate