title: Interaction of neuroprotective substances with human brain superoxide dismutase. An in vitro study.
abstract: Human brain total superoxide dismutase activity (SOD) was assayed in the presence of increasing concentrations of neuroprotectives. Superoxide-dependent nitrobluetetrazolium (NBT) reduction served as control for direct radical interaction of these substances. High concentrations of the dopamimetic substances L-DOPA slightly and the monoamine oxidase B inhibitor selegiline more effectively inhibit SOD activity. The MAO-B inhibitor RO 16-6491 (N-(2-aminoethyl)-4-chlorobenzamide hydrochloride) has no effect on SOD enzyme activity. Reduced glutathione stimulates SOD activity. Moreover it exhibits slight activity in scavenging radicals in vitro. Oxidized glutathione and vitamin E are unable to do so. Ascorbic acid mimics the activity of reduced glutathione, but directly interacts with NBT reduction. Thioctic acid shows no effect on SOD activity but stimulates superoxide-dependent NBT reduction. The Ginkgo biloba extract EGb 761 is highly active in inhibiting superoxide-dependent NBT reduction as well as SOD activity.
authors: Department of Psychiatry, Clinical Neurochemistry, University of Würzburg, Federal Republic of Germany.
authors: W
authors: W
authors: Gsell
authors: N
authors: N
authors: Reichert
authors: M B
authors: MB
authors: Youdim
authors: P
authors: P
authors: Riederer
mesh_terms: D000328:Adult; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D015259:Dopamine Agents / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D009410:Nerve Degeneration / Q000502:physiology*; D018696:Neuroprotective Agents / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000378:metabolism; D013481:Superoxides
chemical_list: D015259:Dopamine Agents; D007202:Indicators and Reagents; D018696:Neuroprotective Agents; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase