title: Effects of microcystin-LR on the partial reactions of the Na(+)-K+ pump of the gill of carp (Cyprinus carpio Linneo).
abstract: Several partial reactions of the Na(+)-K+ pump enzyme were studied in a microsomal fraction derived from the gill of carp (Cyprinus carpio Linneo). We tested the effect of three toxins [(i) microcystin-LR, (ii) microcystin-LR-like toxin component isolated from Microcystis aeruginosa culture and (iii) okadaic acid] on the phosphorylation, ouabain binding and ATPase activity of the Na(+)-K+ pump. The K(+)-dependent hydrolysis of the Na(+)-dependent phosphorylation of Na(+)-K+ pump, as well the release of bound ouabain were inactivated in direct proportion to the amount of each toxin treatment. These results indicate that these toxins not only block the hydrolysis of phosphorylated protein at serine and threonine residues, but also inhibit the aspartic dephosphorylation step of the sodium pump enzymes. This inactivation could disrupt the ion homeostasis of the internal medium by blocking the gill function. The blockage of gill activity could be the cause of the massive fish death during blooms of M. aeruginosa.
authors: Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors: F
authors: F
authors: Zambrano
authors: E
authors: E
authors: Canelo
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002347:Carps; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D005880:Gills / Q000187:drug effects* / Q000201:enzymology; D006868:Hydrolysis; D007202:Indicators and Reagents / Q000378:metabolism; D008387:Marine Toxins; D052998:Microcystins; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism; D017830:Octoxynol / Q000737:chemistry; D019319:Okadaic Acid / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism; D010042:Ouabain / Q000378:metabolism / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000633:toxicity*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010766:Phosphorylation / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D008387:Marine Toxins; D052998:Microcystins; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010456:Peptides, Cyclic; D019319:Okadaic Acid; C008644:nitrophenylphosphate; D010042:Ouabain; D017830:Octoxynol; D010749:Phosphoprotein Phosphatases; D000254:Sodium-Potassium-Exchanging ATPase; C057862:cyanoginosin LR