Phospholipid metabolism induced by Clostridium perfringens alpha-toxin elicits a hot-cold type of hemolysis in rabbit erythrocytes.
GTP and AIF4- significantly stimulated the late phosphatidic acid (PA) formation induced by Clostridium perfringens alpha-toxin in rabbit erythrocyte lysates. Pertussis toxin blocked the PA production. AIF4- markedly enhanced phosphatidylethanol production induced by alpha-toxin in the presence of ethanol. GTP[gamma S] stimulated the PA formation and hemolysis induced by alpha-toxin, and GDP[beta S] inhibited them. An H-to-G mutation at position 126 (H126G) induced the PA formation and hemolysis in a Co2+ concentration-dependent manner. H148G induced neither the PA formation nor hemolysis. These results suggest that the toxin-induced hemolysis is due to activation of phospholipid metabolism systems through GTP-binding protein.
Ochi
S
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Hashimoto
K
K
Nagahama
M
M
Sakurai
J
J
D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000633:toxicity*; D002135:Calcium-Binding Proteins; D003016:Clostridium perfringens / Q000472:pathogenicity*; D003035:Cobalt / Q000494:pharmacology; D003080:Cold Temperature; D004910:Erythrocyte Membrane / Q000502:physiology*; D005459:Fluorides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006358:Hot Temperature; D010743:Phospholipids / Q000097:blood*; D011817:Rabbits; D013329:Structure-Activity Relationship; D010738:Type C Phospholipases
D017607:Aluminum Compounds; D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D006150:Guanine Nucleotides; D010743:Phospholipids; D003035:Cobalt; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens; D019204:GTP-Binding Proteins; D005459:Fluorides; C032311:aluminum fluoride