Protection of mouse bone marrow by Si-WU-Tang against whole body irradiation.
The ability of Si-Wu-Tang to eliminate bone marrow damage after radiation was assessed using both a clonogenic assay (survival of colony forming units spleen--CFUs) of stem cell survival and a hematopoietic functional assay including the changes of hemograms and hematocrit. Cell survival curves and dose-response curves for radiation alone and Si-Wu-Tang administration with radiation were constructed over the dose range of 1 to 9 Gy. Si-Wu-Tang was given 7 days before irradiation at a consecutive fractionated dose of 20 mg/20 g body weight. The radioresistance of stem cells treated by Si-Wu-Tang was higher than that of those treated by irradiation alone. However, the anti-radiation effect of leukocytes was not as significant as that of erythrocytes, thrombocytes and hematocrit. These data suggest that the anti-radiation effect by Si-Wu-Tang is dependent on dose and will be less after 4 Gy irradiation. Hemograms with a short turnover period, such as leukocytes, may be less affected after Si-Wu-Tang administration.
Hsu
H Y
HY
Department of Radiotherapy and School of Pharmacy, Kaohsiung Medical College, Taiwan, R.O.C.
Ho
Y H
YH
Lin
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CC
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004912:Erythrocytes / Q000187:drug effects / Q000528:radiation effects; D006400:Hematocrit; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal; D011837:Radiation-Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013234:Stem Cells / Q000187:drug effects / Q000528:radiation effects; D013624:Taiwan; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011837:Radiation-Protective Agents