Induction of tetraploid DNA content by mutant simian virus 40 T antigen that has reduced complex formation with p53.
The 402 mutants (DE, DH, DN) of simian virus (SV) 40 form reduced levels of p53-T antigen complexes or no complexes in lytically infected cells (CV-1 cells) relative to wild-type (wt) virus when assayed by immunoprecipitation. When CV-1 cells were infected with the 402 mutants, the cells were induced into multiple rounds of DNA synthesis without mitosis, resulting in a large population of cells with > G2 (tetraploid) DNA content similar to wt virus. The levels of T antigen and p53 per cell that were determined by flow cytometry were similar to wt lytically infected cells, with the levels of T antigen increasing as the infection proceeded. The p53 increased as the levels of T antigen increased, similar to a wt infection. These studies demonstrate that, in lytically infected cells with reduced p53-T antigen complex formation, the cells are induced into multiple rounds of DNA synthesis.
Lehman
J M
JM
Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College, New York 12208, USA.
Perry
M B
MB
Laffin
J
J
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002453:Cell Cycle; D004279:DNA, Viral / Q000096:biosynthesis*; D009154:Mutation; D011003:Ploidies; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53