Biochemical characteristics of a human myeloid leukemia cell differentiation factor.
A human lymphocyte-derived maturation-inducing activity, which mediates the differentiation of human myeloid leukemia cells HL-60 to monocytic cells, was purified and characterized. The maturation inducer is a single polypeptide with a molecular mass of 54,300 Daltons. A Pl range of 8.0-8.5 was determined along with the amino acid composition of the inducer. In cell cultures, the purified inducer mediated a growth reduction and a differentiation of leukemia cells to mature monocytes-macrophages, possessing complement receptors and phagocytic capacity.
Xu
W
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Department of Medicine, New York Medical College, Valhalla 10595, USA.
Chiao
J W
JW
D000596:Amino Acids / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D009000:Monocytes / Q000473:pathology; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D016622:Silver Staining; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D017077:Culture Media, Conditioned; D010455:Peptides