Ontogenic development of membrane lipids in the chick optic lobe.
The developmental profiles of the lipid composition and their de novo synthesis and remodelling in the optic lobe of the chicken were studied. The 32P incorporation to phospholipids showed an active de novo synthesis mainly of phosphatidylinositol and of a particular fraction of phosphatidylcholine during the early stages of the embryo development, concomitantly with the beginning of synaptogenesis. This de novo synthesis of phospholipids strongly increased at hatching. On the other hand, phosphatidylinositol presented an active lipid exchange (acylation-deacylation) in the early stages of embryogenesis, indicating a strong incorporation of 14C-arachidonic acid during this period, followed by a fast drop in specific activity. Two different fractions of phosphatidylcholine were isolated by high-performance thin-layer chromatography with a different profile of fatty acid composition, disclosing their different physicochemical behavior, metabolic activities and evolution during embryogenesis. 32P incorporation into phosphatidylethanolamine remained very low during the earliest stages of embryogenesis, showing an increase when the process of synaptogenesis began, until hatching, when radioactivity reached a plateau. 14C-arachidonic acid incorporation into phosphatidylethanolamine was minimal. Furthermore, the phosphatidylethanolamine pool was progressively enriched in its ethanolamine plasmalogen throughout the development. Chromatographic analysis of lipid extracts showed the presence of cerebroside traces after 16 days of embryo incubation. At hatching, a remarkable increase in non-hydroxylated cerebrosides was observed concurrently with the appearance of hydroxylated ones. These glycosphingolipids, as well as the sulfatides, were markedly increased in the lipid extracts of optic lobes of adult animals, indicating the progressive development and maturity of the myelin sheath.
Rivas
E A
EA
Instituto de Biologia Celular, Facultad de Medicina, UBA.IQUIFIB.CONICET., Buenos Aires, Argentina.
Fernández-Tomé
M C
MC
Biancotti
J C
JC
Sterin-speziale
N B
NB
Fiszer de Plazas
S
S
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis; D008563:Membrane Lipids / Q000096:biosynthesis*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D013570:Synaptic Membranes / Q000378:metabolism
D001095:Arachidonic Acids; D005227:Fatty Acids; D006028:Glycosphingolipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids; D002784:Cholesterol