Genomic variation in Trypanosoma cruzi clonal cultures.
Spontaneous changes in restriction DNA profiles and pulsed-field gel electrophoresis (PFGE) patterns, along with a concomitant loss of infectivity, were observed in infective clones of Trypanosoma cruzi strain Y either following a number of passages during the exponential growth phase of after subcloning in liver infusion tryptone (LIT) medium using as the probe a genomic fragment of the parasite (pMYP16), indicating naturally occurring rearrangements of DNA sequences. No variation could be detected when the genomic DNA was probed with conserved T. cruzi tubulin and actin genes. There was no correlation between such rearrangements and the life-cycle forms of the parasites, since trypomastigote forms showed the same karyotype and hybridization patterns as did epimastigote forms. The variations observed could be reverted and infectivity, recovered after inoculation of the parasites in newborn mice.
Alves
A M
AM
Laboratório de Fisiologia Celular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
de Almeida
D F
DF
von Krüger
W M
WM
D000199:Actins / Q000235:genetics; D000818:Animals; D015139:Blotting, Southern; D014355:Chagas Disease / Q000469:parasitology*; D016054:DNA, Protozoan; D017125:Genes, Protozoan; D014644:Genetic Variation; D018503:Genome, Protozoan; D007621:Karyotyping; D051379:Mice; D014349:Trypanosoma cruzi / Q000235:genetics*; D014404:Tubulin / Q000235:genetics
D000199:Actins; D016054:DNA, Protozoan; D014404:Tubulin