PAF-acetylhydrolase activity and PAF levels in pancreas and plasma of well-fed, diabetic and fasted rat.
PAF-AH activity was determined in pancreas homogenates. The enzyme activity was moderately stable upon storage at -20 degrees C. PAF and lyso-PAF were identified in rat pancreas and their concentrations were determined. PAF levels and PAF-AH activity were compared in the pancreatic tissue and plasma of three different groups of animals: well-fed, STZ-induced diabetic and fasted rats. The concentration of PAF in the pancreas of fasted rats was ten fold lower as compared with that of the well-fed or the diabetic animals. The last two groups had similar pancreatic PAF concentration. PAF levels in the plasma of fasted rats were seven fold lower than those of well-fed or diabetic rats, which were found to be similar. The enzyme PAF-AH had the highest activity in the pancreas of well-fed rats. On the contrary, the enzyme seems to be more active in the plasma of fasted as compared with diabetic and well-fed animals.
Trapali
M
M
Department of Chemistry (Biochemistry), University of Athens, Greece.
Mavri-Vavayanni
M
M
Siafaka-Kapadai
A
A
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000201:enzymology*; D005215:Fasting; D008297:Male; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D013311:Streptozocin; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase