Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation.
Using the theoretical model for DNA curvature, we analyzed a set of fragments with a curved insert located in circular permutation. The theoretical permutation analysis of each of the cyclically located fragments reveals the presence of a shifting molecular bend locus. The delineation of the molecular bend locus associated with the fragments obtained by a second permutation helps in providing an explanation for the differential mobility behavior of the fragments.
Nair
T M
TM
Chemical Engineering Division, National Chemical Laboratory, Pune, India.
Madhusudan
K
K
Nagaraja
V
V
Kulkarni
B D
BD
Majumdar
H K
HK
Singh
R
R
D000818:Animals; D001483:Base Sequence; D004270:DNA, Circular; D018105:DNA, Kinetoplast / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007891:Leishmania / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data
D004270:DNA, Circular; D018105:DNA, Kinetoplast