[Evaluation of platelet membrane structure in patients with chronic glomerulonephritis (CGN)].
The structure of platelet membrane determines platelet surface glycoprotein receptors and correct cell function. Meanwhile different diseases and drugs may induce changes in this structure. The aim of this study was to determine structure of platelet lipid bilayer in patients with chronic glomerulonephritis (CGN) in comparison to control group. Studies were carried out in 30 patients with nephrotic syndrome (NS) and 30 patients without this syndrome and 15 controls. Electron spin resonance phenomenon with spin labelling, was used for estimation of platelet membrane structure. CGN patient platelets showed lower order parameter A/C and increased rotation correlation time tau in comparison with healthy subjects. It indicated abnormal platelet membrane structure in these patients. The negative significant correlation between parameter A/C and plasma fibrinogen concentration of NS patients has been found (r = -0.5; p < 0.05). Additionally the value of an order parameter (A/C) has significantly positively correlated with serum triglycerides concentration in patients with NS and spontaneous platelet aggregation (r = 0.52, p < 0.05). Conclusion. 1. CGN patient platelets have revealed disorders of lipid bilayer structure. 2. These disorders could be responsible for decreased platelet aggregation observed in CGN patients in comparison to healthy controls.
Zdrojewski
Z
Z
Z Kliniki Chorób Nerek Instytutu Chorób, Wewnétrznych Akademii Medycznej, w Gdańsku.
Nowak
J
J
Raszeja-Specht
A
A
Skibowska
A
A
Kustosz
J
J
Rutkowski
B
B
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D002908:Chronic Disease; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D005921:Glomerulonephritis / Q000097:blood*; D006801:Humans; D008051:Lipid Bilayers / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000032:analysis*; D010974:Platelet Aggregation; D013113:Spin Labels; D014280:Triglycerides / Q000097:blood
D008051:Lipid Bilayers; D010743:Phospholipids; D013113:Spin Labels; D014280:Triglycerides; D005340:Fibrinogen
Ocena struktury błony plazmatycznej płytek krwi chorych z przewlekłym kłebuszkowym zapaleniem nerek (PKZN).