Response of a protein disulfide isomerase-like activity of transitional endoplasmic reticulum to all-trans retinol.
Isolated membrane fractions enriched in vesicles of transitional endoplasmic reticulum from rat liver exhibited protein disulfide isomerase-like activity of low specific activity. Activity was measured as the ability to restore activity to reduced, denatured and oxidized (scrambled) RNase. Submicromolar concentrations of retinol either stimulated or inhibited this activity depending on the composition of the redox buffer. In the presence of 1 microM reduced glutathione, micromolar concentrations of retinol stimulated the activity while higher or lower concentrations were less effective. With scrambled RNase, retinol was largely without effect in the absence of reduced glutathione or in the presence of oxidized glutathione. In the presence of NADH, retinol inhibited the protein disulfide-like activity over the same range of concentrations where retinol stimulated in the presence of reduced glutathione. These responses were observed with scrambled and inactive RNase and with reduced and inactive RNase as substrates. Also inhibited by retinol in these membrane preparations was their ability to oxidize NADH. Thus the retinol-modulated protein disulfide isomerase activity appears to correlate with the presence in transitional endoplasmic reticulum of an activity capable of oxidizing NADH in the presence of potassium cyanide that also was inhibited by submicromolar concentrations of retinol.
Jacobs
E
E
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA.
Morré
D J
DJ
de Cabo
R
R
Sweeting
M
M
Morré
D M
DM
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D007535:Isomerases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D011489:Protein Denaturation; D019704:Protein Disulfide-Isomerases; D051381:Rats; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology*
D009097:Multienzyme Complexes; D014801:Vitamin A; D014212:Tretinoin; D000242:Cyclic AMP; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D012259:Ribonuclease, Pancreatic; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; D005978:Glutathione; D019803:Glutathione Disulfide