Partial N-terminal amino acid sequence of the anti-neoplastic urinary protein (ANUP) and the anti-tumour effect of the N-terminal nonapeptide of the unique cytokine present in human granulocytes.
The N-terminal amino acid sequence of the anti-neoplastic urinary protein (ANUP), a unique cytokine present in human granulocytes, was determined to be: Pyroglu-Leu-Lys-X-Tyr-Thr-X-Lys-Glu-Pro-Met-Thr-Ser(Thr)-Ala-Ala... This sequence showed no significant homology with any other protein when used in database searches. Furthermore, a synthetic nonapeptide corresponding to the first nine residues, with Cys in positions 4 and 7, was found to be a biologically active in vitro anti-tumour agent. An alternate method for the purification of ANUP to that previously reported is also presented. This method involves differential Amicon Diaflo membrane filtration.
Ridge
R J
RJ
Woods Hole Oceanographic Institution, Massachusetts, 02543, USA.
Sloane
N H
NH
D000595:Amino Acid Sequence; D000950:Antigens, Ly; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000097:blood*; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007931:Leucyl Aminopeptidase; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D011751:Pyroglutamyl-Peptidase I; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D014462:Ultrafiltration; D014568:Urokinase-Type Plasminogen Activator
D000950:Antigens, Ly; D000970:Antineoplastic Agents; D016207:Cytokines; D010446:Peptide Fragments; D011506:Proteins; C048963:SLURP1 protein, human; D007931:Leucyl Aminopeptidase; D011751:Pyroglutamyl-Peptidase I; D014568:Urokinase-Type Plasminogen Activator