Lack of correlation between NADPH-oxidase priming and elevated alkaline phosphatase activity in cord blood neutrophils.
Human neutrophils were isolated from cord blood drawn after Caesarean section deliveries without labour and from peripheral blood from healthy adults. The alkaline phosphatase (ALP) activity in the cell populations was compared with the release of respiratory burst products after activation with the chemoattractant n-formyl-methionyl-leucyl-phenylalanine (fMLP). In contrast to cord blood neutrophils obtained from babies after normal vaginal deliveries, the Caesarean section neutrophils were not metabolically primed. However, like the neutrophils from vaginally delivered babies, the Caesarean section neutrophils showed an increased ALP activity compared to adult control neutrophils. These findings indicate that priming of cord blood neutrophils appears to be induced by labour and can be dissociated from increased ALP activity.
Khalfan
L
L
Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Karlsson
A
A
Lundqvist
H
H
Björkstén
B
B
Dahlgren
C
C
D000328:Adult; D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D004789:Enzyme Activation; D004790:Enzyme Induction; D005312:Fetal Blood / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007231:Infant, Newborn; D019255:NADPH Oxidases / Q000096:biosynthesis*; D018375:Neutrophil Activation; D009504:Neutrophils / Q000201:enzymology*
D019255:NADPH Oxidases; D000469:Alkaline Phosphatase