[Immune and biochemical analysis of protein of homogenates of three different kinds of skin].
The amount and constitution of protein, its ultraviolet photo-absorption ability, and esterase in homogenates of human cadaveric and guinea pig skin (each consisted of newborn, fresh adult and stored adult skin) were assayed by using ultraviolet spectrophotometer, polyacrylamide gel electrophoresis and sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiment shows that immune and biochemical analysis of protein of three different skin groups are different. The biochemical components of cadaveric and guinea pig skin protein and esterase in newborn skin group are lowest. The changes in adult skin stored at -196 degrees C are less obvious as compared with that in fresh adult skin.
Jia
X
X
304th Hospital of People's Liberation Army, Beijing.
Zhu
Z
Z
Li
C
C
D000818:Animals; D000831:Animals, Newborn; D004950:Esterases / Q000378:metabolism*; D005333:Fetus; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D011506:Proteins / Q000032:analysis; D012867:Skin / Q000276:immunology* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins; D004950:Esterases