Prevalence and turnover of peptidylglycine alpha-amidating monooxygenase mRNA in atrial cardiomyocytes.
Peptidylglycine alpha-amidating monooxygenase (PAM), the enzyme responsible for the alpha-amidation of neuroendocrine peptides, is more prevalent in the atrium of the heart than in pituitary or brain. RNase protection assays indicate that PAM transcripts account for approximately 0.5% of the mRNA in the neonatal atrium and 0.06% of the mRNA in the neonatal ventricle. In primary atrial cardiomyocyte cultures PAM mRNA turns over slowly, with a half-life of approximately 20 h. Levels of PAM mRNA in primary atrial cardiomyocytes are increased to 16.5% of control upon treatment with dexamethasone and decreased to 63% of control upon treatment with thyroid hormone.
Maltese
J Y
JY
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore MD 21205, USA.
Oyarce
A M
AM
Eipper
B A
BA
D000818:Animals; D000831:Animals, Newborn; D016375:Antisense Elements (Genetics); D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006325:Heart Atria; D006352:Heart Ventricles; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D009097:Multienzyme Complexes; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D010902:Pituitary Gland / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000494:pharmacology
D016375:Antisense Elements (Genetics); D009097:Multienzyme Complexes; D012333:RNA, Messenger; D014284:Triiodothyronine; D003907:Dexamethasone; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase