Estimation of single-strand breaks induced in the dried film of DNA by high energy alpha particle from a cyclotron.
DNA extracted and purified from Vibrio cholerae OGAWA 154 cells and prepared in the form of a dry thin film was exposed in air to a beam of alpha particles obtained from a Variable Energy Cyclotron. The number of single-strand breaks per DNA unit exhibited a linear dose-effect relationship indicating the occurrence of single-hit kinetics. The efficiency and yield of alpha-induced single-strand breaks were approximately 72 eV/break and 1.39 respectively.
Basak
J
J
Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta, India.
D000512:Alpha Particles; D017357:Cyclotrons; D004249:DNA Damage; D004269:DNA, Bacterial / Q000737:chemistry / Q000528:radiation effects*; D004277:DNA, Single-Stranded; D014734:Vibrio cholerae
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded