title: M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin.
abstract: Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.
authors: Department of Development and Genetics, Babraham Institute, Cambridge, UK.
authors: D
authors: D
authors: Horsley
authors: A
authors: A
authors: Hutchings
authors: G W
authors: GW
authors: Butcher
authors: P B
authors: PB
authors: Singh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000276:immunology; D004331:Drosophila melanogaster / Q000235:genetics*; D022041:Euchromatin; D019143:Evolution, Molecular; D005796:Genes; D017344:Genes, Insect; D006570:Heterochromatin / Q000737:chemistry; D007399:Interphase; D051379:Mice / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D022041:Euchromatin; D006570:Heterochromatin; D000090266:Chromobox Protein Homolog 5