A new non-HLA multigene family associated with the PERB11 family within the MHC class I region.
In an effort to initiate steps designed to characterize the idiopathic hemochromatosis disease gene, the HLA-A/HLA-F region where this gene is in disequilibrium linkage with some polymorphic markers has been overlapped by a yeast artificial chromosome (YAC) contig. In order to achieve the physical mapping of these YACs and of the corresponding genomic region, we subcloned one of the YACs involved. A computer-assisted analysis of the sequence of one subclone led to the isolation of a potential exon that proved to belong to a new expressed messenger named HCGIX. After Southern blot analysis, the corresponding cDNA clone was found to belong to a new multigene family whose members are dispersed throughout the HLA class I region and are closely associated with members of another recently described multigene family designated PERB11. The data reported here suggest that these two multigene families form a cluster that have been dispersed together throughout the telomeric part of the major histocompatibility complex and have been involved in the genesis of this human class I region.
Pichon
L
L
Department of Biochemistry and Molecular Biology, UPR 41 CNRS "Recombinaisons Génétiques" Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes Cedex, France.
Hampe
A
A
Giffon
T
T
Carn
G
G
Legall
J Y
JY
David
V
V
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003360:Cosmids; D016208:Databases, Factual; D004386:Duodenum / Q000737:chemistry; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D009132:Muscles / Q000737:chemistry; D012313:RNA / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid
D012313:RNA