Ultrastructural localization of G-protein GS in the lateral wall of the guinea pig cochlear duct.
Immunocytochemical localization of a GTP-binding protein, Gs, in the various cells of the lateral wall of guinea pig cochlear duct was investigated using a post-embedding immunogold method with antibody raised against a synthetic decapeptide (RMHLRQYELL) encoding the C-terminus of the alpha-subunit of Gs. In the stria vascularis, labeling was observed on the basolateral membrane infoldings of marginal cells, on the juxtaposed membrane of intermediate cells, and on the cell membrane of basal cell. In contrast, no significant labeling was observed on the luminal membrane of marginal cells. Immunoreactivity also was detected on the cell membranes of various other cells. These include spiral prominence epithelial cells, fibrocytes of spiral ligament, external sulcus cells, and epithelial and mesothelial cells of Reissner's membrane. Adenylylcyclase has been functionally implicated in some of the cell types with membranes labeled in this study. The significance of these findings is briefly discussed.
Mizuta
K
K
Laboratory of Cellular Biology, National Institute on Deafness and Other Communication Disorders, Bethesda, MD 20892, USA.
Iwasa
K H
KH
Simonds
W F
WF
Tachibana
M
M
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D004848:Epithelium / Q000737:chemistry; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D010455:Peptides / Q000276:immunology; D015398:Signal Transduction / Q000502:physiology; D013136:Spiral Ganglion / Q000737:chemistry; D013316:Stria Vascularis / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure
D010455:Peptides; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases