Localization of mRNA coding for the three subtypes of atrial natriuretic factor (ANF) receptors in rat anterior pituitary gland cells.
Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor-A, -B and -C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was detected by in situ hybridization. The amounts of ANFR-A and -B mRNA were found to be similar, and to be twice that of ANFR-C mRNA. At the ultrastructural level, the three types of ANFR mRNA were expressed in three anterior pituitary cell types, namely lactotrophs, corticotrophs, and gonadotrophs, identified by their hormonal content. No signal was revealed in somatotrophs or thyrotrophs. The different forms of mRNA were similar in terms of subcellular localization: in the cytoplasmic matrix and the nuclear euchromatin. These data indicate that the anterior pituitary is an important target tissue for ANF action.
Grandclément
B
B
CNRS URA 1459, Institut Pasteur de Lyon, France.
Brisson
C
C
Bayard
F
F
Tremblay
J
J
Gossard
F
F
Morel
G
G
D000818:Animals; D009320:Atrial Natriuretic Factor; D001483:Base Sequence; D003001:Cloning, Molecular; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000096:biosynthesis; D006162:Guanylate Cyclase / Q000032:analysis / Q000096:biosynthesis*; D017403:In Situ Hybridization; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D000099242:Atrial Natriuretic Factor Receptor B
D009320:Atrial Natriuretic Factor; D013006:Growth Hormone; D006162:Guanylate Cyclase; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D011994:Recombinant Proteins; D000099242:Atrial Natriuretic Factor Receptor B; C472610:atrial natriuretic peptide, rat; C091715:atrial natriuretic factor receptor A; C073616:atrial natriuretic factor receptor C