Glycophorin as a receptor for Sendai virus.
Glycophorin A was reconstituted into large unilamellar vesicles of egg phosphatidylcholine by detergent dialysis. The observed overall rate of Sendai virus fusion increased approximately 4-fold between 0 and 0.006 mol % glycophorin, roughly proportional to the glycophorin content. However, no further increase in rate was observed at 0.02 mol % glycophorin. Treatment of reassembled glycophorin-liposomes with neuraminidase resulted in a significant decrease in the percent of viral fusion, confirming that the presence of sialic acid residues on glycophorin is essential for its role as a receptor. The sialic acid-containing glycolipid, the ganglioside GD1a, was also incorporated into phosphatidylcholine liposomes, either in addition to or in place of glycophorin A. Comparing, on the basis of sialic acid content, liposomes containing either glycophorin or GD1a, comparable rates and extents of fusion were found. However, on a molar basis glycophorin is much more effective. It was found that the addition of GD1a to glycophorin-containing liposomes only slightly increased the rate of fusion. This was largely due to an increase in the percent of virions capable of fusing.
Wybenga
L E
LE
Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
Epand
R F
RF
Nir
S
S
Chu
J W
JW
Sharom
F J
FJ
Flanagan
T D
TD
Epand
R M
RM
D005732:Gangliosides / Q000494:pharmacology; D006021:Glycophorins / Q000378:metabolism* / Q000494:pharmacology; D008081:Liposomes / Q000378:metabolism; D008561:Membrane Fusion; D009439:Neuraminidase / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*
D005732:Gangliosides; D006021:Glycophorins; D008081:Liposomes; D010713:Phosphatidylcholines; D011991:Receptors, Virus; C021298:ganglioside, GD1a; D009439:Neuraminidase