title: Role of the intermembrane-space domain of the preprotein receptor Tom22 in protein import into mitochondria.
abstract: Tom22 is an essential component of the protein translocation complex (Tom complex) of the mitochondrial outer membrane. The N-terminal domain of Tom22 functions as a preprotein receptor in cooperation with Tom20. The role of the C-terminal domain of Tom22, which is exposed to the intermembrane space (IMS), in its own assembly into the Tom complex and in the import of other preproteins was investigated. The C-terminal domain of Tom22 is not essential for the targeting and assembly of this protein, as constructs lacking part or all of the IMS domain became imported into mitochondria and assembled into the Tom complex. Mutant strains of Neurospora expressing the truncated Tom22 proteins were generated by a novel procedure. These mutants displayed wild-type growth rates, in contrast to cells lacking Tom22, which are not viable. The import of proteins into the outer membrane and the IMS of isolated mutant mitochondria was not affected. Some but not all preproteins destined for the matrix and inner membrane were imported less efficiently. The reduced import was not due to impaired interaction of presequences with their specific binding site on the trans side of the outer membrane. Rather, the IMS domain of Tom22 appears to slightly enhance the efficiency of the transfer of these preproteins to the import machinery of the inner membrane.
authors: Institut für Physiologische Chemie, Physikalische Biochemie, Zellbiologie der Universität München, Munich, Germany.
authors: D A
authors: DA
authors: Court
authors: F E
authors: FE
authors: Nargang
authors: H
authors: H
authors: Steiner
authors: R S
authors: RS
authors: Hodges
authors: W
authors: W
authors: Neupert
authors: R
authors: R
authors: Lill
mesh_terms: D001483:Base Sequence; D001692:Biological Transport; D002451:Cell Compartmentation; D017931:DNA Primers / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009492:Neurospora crassa; D011498:Protein Precursors / Q000378:metabolism*; D011956:Receptors, Cell Surface; D017384:Sequence Deletion; D013329:Structure-Activity Relationship
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011498:Protein Precursors; D011956:Receptors, Cell Surface