Rapid removal of unincorporated label and proteins from DNA sequencing reactions.
This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (including DNA sequencing) when the level of background should be as low as possible. Also, this method is effective in removal of proteins from DNA sequencing reactions.
Kaczorowski
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McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706, USA.
Sektas
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D000255:Adenosine Triphosphate / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification*; D007553:Isotope Labeling; D009841:Oligonucleotides / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods*
D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D011506:Proteins; D000255:Adenosine Triphosphate; D004247:DNA