Accumulation of inositol 1,4,5-triphosphate after cholinergic stimulation of human lymphocytes.
The interrelationships between the immune and neurohormonal system are the subject of this work. The formation of inositol 1,4,5-triphosphate (IP3) in response to cholinergic stimulation was studied in the human peripheral blood mononuclear cells (PBMC) and lymphocytes from the lymphoblastoid cell lines Jurkat and Raji. The cells were stimulated with mitogen and then treated with cholinergic agonist-carbachol or nicotine. The radioreceptor assay was used to determine the amount of IP3 in all studied lymphocytes. The results, combined with the presence of muscarinic and nicotinic receptors on the surface of these lymphocytes, suggest that the parasympathetic nervous system is involved in the immune-neurohormonal crosstalk.
Bany
U
U
Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland.
Laskowska-Bozek
H
H
Ryzewski
J
J
D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009538:Nicotine / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D009538:Nicotine; D015544:Inositol 1,4,5-Trisphosphate; D002217:Carbachol