[Value of media containing chromogenic substrates for the identification and the listing of urinary bacteria].
Performance in terms of enumeration, growth and identification with a new culture medium (CPS ID2, bioMérieux) was evaluated using 405 urine samples and 244 collection strains. The CPS ID2 medium contains chromogenic substrates enabling the detection of 4 bacterial enzymes which leads to the identification of colonies: Escherichia coli, Proteus mirabilis, Proteus-Morganella-Providencia and enterococci. No discrepancies in the enumeration were observed with the collected strains and urine samples. Sensibility and specificity of the identification of Proteae taxa (TDA +) and enterococcal taxa (Gram positive cocci and beta-glucosidase +) was 100% using urine samples and 95.1% of E. coli strains (beta-glucuronidase +, indole +) are detected. Routine use of this new medium for the bacterial examination of urine is satisfactory and an ultra-violet lamp is no longer required for reading as was the case previously with media containing fluorogenic substrates.
Martin
C
C
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière, CHU Dupuytren, Limoges, France.
Orenga
S
S
Doleans
F
F
Denis
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001437:Bacteriuria / Q000382:microbiology*; D002863:Chromogenic Compounds; D015169:Colony Count, Microbial; D003470:Culture Media; D004755:Enterobacteriaceae / Q000187:drug effects / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D011512:Proteus Infections / Q000175:diagnosis / Q000382:microbiology; D011513:Proteus mirabilis / Q000187:drug effects / Q000302:isolation & purification*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology
D000900:Anti-Bacterial Agents; D002863:Chromogenic Compounds; D003470:Culture Media
Intérêt des milieux contenant des substrats chromogènes pour l'identification et la numération des bactéries urinaires.