Detection of ileal symbiont intracellularis in porcine faecal samples by polymerase chain reaction.
Ileal Symbiont Intracellularis (ISI), the organism causing proliferative enteritis (PE) in pigs was detected in faeces by the application of polymerase chain reaction (PCR). The assay based on a 319 base pair DNA fragment was used on faecal and mucosal samples derived from pigs either affected or unaffected with PE. As few as 10(3) ISI could be detected in pig faeces spiked with ISI. No amplification product was detected in the faeces of unaffected pigs but faeces of confirmed clinical cases were positive. This method offers an accurate, sensitive, easy to perform alternative to monoclonal antibody tests or histological examination post-mortem for the presence of ISI in pig herds.
McCormick
B M
BM
Department of Agriculture, Bendigo, Australia.
Hasse
D
D
Monckton
R P
RP
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004751:Enteritis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D004269:DNA, Bacterial