Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India.
Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and P types, of which P8G1 and P4G2 (33% each) genotypes were predominant. The P6 genotype was detected in four children with diarrhea.
Husain
M
M
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
Seth
P
P
Dar
L
L
Broor
S
S
D000208:Acute Disease; D001483:Base Sequence; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*
D017931:DNA Primers; D012367:RNA, Viral