Cell lineage analysis of the Drosophila peripheral nervous system.
The peripheral nervous system (PNS) of Drosophila provides a very well-characterized model system for studying the genes involved in basic processes of neurogenesis. Because of its simplicity and stereotyped pattern, each cell of the PNS can be individually identified and the phenotypic consequences of mutations can be studied in detail. Thus, some of the genetic mechanisms leading to the formation of type I sensory organs, the external, bristle-type sensory organs (es), and the internal, stretch-receptive chordotonal organs (ch) have been elucidated. Each sensory organ seems to be generated by a stereotyped pattern of cell division of individual ectodermal precursor cells. Recent advances in cell lineage analysis of the PNS have provided a detailed picture of almost all the lineages in the PNS, including those giving rise to the type II sensory neurons, also known as multiple dendritic (md) neurons. This knowledge will be instrumental in the precise characterization of the phenotypes associated with mutations in known and new genes and their interactions which determine cell fate decisions during neurogenesis. Here, we describe and compare three recently developed methods by which cell lineages have been assessed: single cell transplantation, bromodeoxyuridine (BrdU) incorporation studies, and the flp/FRT recombinase system from yeast. In the light of a more complete knowledge of the PNS lineages, we will discuss the effects of known mutations that alter neuronal cell fates.
Brewster
R
R
Department of Biology, University of Michigan, Ann Arbor, USA.
Bodmer
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D004330:Drosophila / Q000166:cytology / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology; D006735:Horseradish Peroxidase; D006358:Hot Temperature; D008959:Models, Neurological; D009419:Nerve Tissue Proteins / Q000032:analysis; D009420:Nervous System / Q000166:cytology* / Q000196:embryology*; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D009475:Neurons, Afferent / Q000166:cytology / Q000502:physiology; D013045:Species Specificity; D014714:Vertebrates
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D006735:Horseradish Peroxidase; D001973:Bromodeoxyuridine