title: Development of low magnesium-induced spontaneous synchronized bursting and GABAergic modulation in cultured rat neocortical neurons.
abstract: Development of spontaneous synchronized bursting in the early stages of rat neocortical neuronal cultures was studied by whole-cell and extracellular recordings. Neocortical neurons from rat embryos were cultured on planar electrode arrays, and low Mg(2+)-induced spontaneous activity was recorded from 5 to 16 days in vitro (DIV). At 5-6 DIV the current synchronized to the bursting had only a slow component lasting 3-5 s, whereas in older cultures a fast transient component was dominant. A gamma-aminobutyric acid (GABA)A receptor antagonist, bicuculline methiodide, had little effect on the spontaneous activity at 5-6 DIV, whereas in older cultures it had a marked effect on the slow current component. These results suggest a role of GABAergic transmission in the development of synchronized activities.
authors: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: S
authors: S
authors: Watanabe
authors: Y
authors: Y
authors: Jimbo
authors: H
authors: H
authors: Kamioka
authors: Y
authors: Y
authors: Kirino
authors: A
authors: A
authors: Kawana
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000031:analogs & derivatives / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology*; D004594:Electrophysiology; D008274:Magnesium / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D010507:Periodicity; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: C017069:bicuculline methiodide; D005680:gamma-Aminobutyric Acid; D008274:Magnesium; D001640:Bicuculline