Glucocorticoid upregulation of glutamate dehydrogenase gene expression in vitro in astrocytes.
Glutamate, the major excitatory neurotransmitter, is preferentially catabolized in astrocytes by glutamate dehydrogenase (GDH). Treatment of an astrocytic cell line with hydrocortisone (10(-5) M) resulted in increased expression of GDH mRNA. Transfection of the cells with truncated parts of the GDH promoter showed that genomic responsive elements activated by hydrocortisone are localized in the -557/+1 region of the promoter. This control of GDH expression by glucocorticoids may be involved in their protective effect against glutamate excitotoxicity.
Hardin-Pouzet
H
H
INSERM U433, Faculté de Médecine A. Carrel, Lyon, France. hardin@cimacres.univ-lyon1.fr
Giraudon
P
P
Belin
M F
MF
Didier-Bazes
M
M
D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D005969:Glutamate Dehydrogenase / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D015854:Up-Regulation
D005938:Glucocorticoids; D005969:Glutamate Dehydrogenase; D006854:Hydrocortisone