title: Cell adhesion molecules in bladder cancer: soluble serum E-cadherin correlates with predictors of recurrence.
abstract: Sera from 40 patients with newly diagnosed bladder cancer (28 superficial tumours (pTa and pT1) and 12 muscle-invasive tumours) were assessed by enzyme-linked immunosorbent assay (ELISA) to determine the concentrations of soluble E-cadherin (sE-cadherin), soluble E-selectin (sE-selectin), soluble vascular cell adhesion molecule-1 (sVCAM-1) and soluble intercellular adhesion molecule-1 (sICAM-1). Corresponding frozen sections of primary tumour were analysed for E-cadherin expression using the monoclonal antibody, HECD-1 and standard immunohistochemistry. Patients with bladder cancer had significantly higher concentrations of sE-cadherin compared with a control group (P = 0.017). No difference was found between the two groups with regard to sE-selection (P = 0.403), sVCAM-1 (P = 0.942) and sICAM-1 (P = 0.092). High levels of sE-cadherin were related to poor histological grade (P = 0.009), number of superficial tumours at presentation (P = 0.008) and a positive 3 month check cytoscopy in superficial disease (P = 0.036). Abnormal E-cadherin expression was associated with increasing tumour stage (P = 0.009) and grade (P = 0.03). There was no correlation between high levels of soluble E-cadherin in sera and abnormal E-cadherin expression by the tumour (P = 0.077). Elevated levels of sE-cadherin are found in sera of patients with bladder cancer and correlate with known prognostic factors.
authors: Department of Urology, Freeman Hospital, Newcastle upon Tyne, UK.
authors: T R
authors: TR
authors: Griffiths
authors: I
authors: I
authors: Brotherick
authors: R I
authors: RI
authors: Bishop
authors: M D
authors: MD
authors: White
authors: D M
authors: DM
authors: McKenna
authors: C H
authors: CH
authors: Horne
authors: B K
authors: BK
authors: Shenton
authors: D E
authors: DE
authors: Neal
authors: J K
authors: JK
authors: Mellon
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D015820:Cadherins / Q000032:analysis* / Q000097:blood*; D016001:Confidence Intervals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011237:Predictive Value of Tests; D012008:Recurrence; D012016:Reference Values; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1