[Comparative study of minimal inhibiting concentrations of doxycycline, ofloxacin and erythromycin against 18 recent isolates from Chlamydia trachomatis (1994-1995)].
In vitro activity of the 3 antimicrobial agents, against 18 recent isolates of Chlamydia trachomatis: 11 from urethral samples and 7 from endocervical samples, was tested by cell culture technique. HeLa 229 cells maintained in antibiotic-free MEM medium supplemented with 10% fetal calf serum and 2 mM glutamine were seeded into 96-well culture plates. After inoculation with 10(2) to 10(3) inclusion-forming units/ml of each strain of C. trachomatis, the culture medium was replaced by a maintenance medium containing 1 micrograms/ml of cycloheximide and serial two fold dilutions of the antibiotics. After 48 h incubation at 36 degrees C in 5% CO2 atmosphere, cells were fixed and inclusion bodies were stained using fluorescein-conjugated anti-Chlamydia monoclonal antibodies. MICs were defined as the lowest antibiotic concentrations required to inhibit the development of a single inclusion. MICs 90% (mg/l) were 0.054 for doxycyclin, 0.700 for ofloxacin and 0.150 for erythromycin. These results confirm the effective in vitro activity of the three antibiotics tested against C. trachomatis.
Bianchi
A
A
Institut Alfred-Fournier, PARIS, France.
Legouge
R
R
Lefevre
J C
JC
Askienazy-Elbhar
M
M
Joly-Guillou
M L
ML
Pavis
A
A
Sednaoui
P
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002692:Chlamydia trachomatis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004318:Doxycycline / Q000494:pharmacology*; D004917:Erythromycin / Q000494:pharmacology; D066298:In Vitro Techniques; D015242:Ofloxacin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004917:Erythromycin; D015242:Ofloxacin; D004318:Doxycycline
Etude comparative des concentrations minimales inhibitrices de la doxycycline, de l'ofloxacine et de l'érythromycine sur 18 isolats récents (1994-1995) de Chlamydia trachomatis.