Functional characterization of a sialyltransferase-deficient mutant of Neisseria gonorrhoeae.
Previous studies indicate that sialylation of lipopolysaccharide (LPS) by host CMP-N-acetylneuraminic acid (CMP-NANA) catalyzed by bacterial sialyltransferase rendered gonococci resistant to killing by phagocytes, to entry into epithelial cell lines, to killing by immune serum and complement, and to absorption of complement component C3. These results have been confirmed by comparing a sialyltransferase-deficient mutant (strain JB1) with its parent (strain F62) in appropriate tests. In contrast to F62, JB1 was very susceptible to killing by human polymorphonuclear phagocytes in opsonophagocytosis tests and incubation with CMP-NANA did not decrease the level of killing. The inherent resistance of F62 in these tests was probably due to LPS sialylation by CMP-NANA and lactate present in the phagocytes. A JB1 variant expressing the invasion-associated Opa protein was as able to enter Chang human conjunctiva epithelial cells as an Opa-positive variant of F62, suggesting that the sialyltransferase is not required for Opa-mediated entry. After incubation with CMP-NANA, the number of F62 variant gonococci entering cells but not that of JB1 variant gonococci was drastically reduced. Both JB1 and F62 were killed by incubation with rabbit antibody to gonococcal major outer membrane protein, protein I, and human complement, but only F62 was rendered resistant to the killing by incubation with CMP-NANA. Finally, both JB1 and F62 absorbed similar amounts of complement component C3 and the binding was decreased by incubation with CMP-NANA only for the wild type, F62.
Gill
M J
MJ
Department of Infection, Medical School, University of Birmingham, United Kingdom.
McQuillen
D P
DP
van Putten
J P
JP
Wetzler
L M
LM
Bramley
J
J
Crooke
H
H
Parsons
N J
NJ
Cole
J A
JA
Smith
H
H
D000818:Animals; D000907:Antibodies, Bacterial; D001422:Bacterial Adhesion; D002460:Cell Line; D003176:Complement C3 / Q000378:metabolism; D003228:Conjunctiva / Q000166:cytology / Q000382:microbiology; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D006801:Humans; D008070:Lipopolysaccharides; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D009504:Neutrophils; D009895:Opsonin Proteins; D010587:Phagocytosis; D011485:Protein Binding; D011817:Rabbits; D012799:Sialyltransferases / Q000172:deficiency* / Q000235:genetics
D000907:Antibodies, Bacterial; D003176:Complement C3; D008070:Lipopolysaccharides; D009895:Opsonin Proteins; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D012799:Sialyltransferases