HIV-1 nucleocapsid protein induces "maturation" of dimeric retroviral RNA in vitro.
After a retrovirus particle is released from the cell, the dimeric genomic RNA undergoes a change in conformation. We have previously proposed that this change, termed maturation of the dimer, is due to the action of nucleocapsid (NC) protein on the RNA within the virus particle. We now report that treatment of a 345-base synthetic fragment of Harvey sarcoma virus RNA with recombinant or synthetic HIV-1 NC protein converts a less stable form of dimeric RNA to a more stable form. This phenomenon thus appears to reproduce the maturation of dimeric retroviral RNA in a completely defined system in vitro. To our knowledge, maturation of dimeric RNA within a retrovirus particle is the first example of action of an "RNA chaperone" protein in vivo. Studies with mutant NC proteins suggest that the activity depends upon basic amino acid residues flanking the N-terminal zinc finger and upon residues within the N-terminal finger, including an aromatic amino acid, but do not require the zinc finger structures themselves.
Feng
Y X
YX
Retroviral Genetics Section, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201, USA.
Copeland
T D
TD
Henderson
L E
LE
Gorelick
R J
RJ
Bosche
W J
WJ
Levin
J G
JG
Rein
A
A
D000595:Amino Acid Sequence; D015497:HIV-1 / Q000378:metabolism*; D006251:Harvey murine sarcoma virus / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019251:Nucleocapsid / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics; D014158:Transcription, Genetic; D016335:Zinc Fingers
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D011994:Recombinant Proteins