A new method for the voltammetric response of hemoglobin.
Hemoglobin (Hb) could give a voltammetric response after it was acted on by the surfactant dodecyl benzene sodium sulfonate (DBS) or sodium dodecyl sulfate (SDS). The mechanism might be the enhancement of the adsorption process when there was surfactant in the protein solution. Moreover, the macromolecules might open their electroactive sections after they interacted with the hydrophobic groups of the surfactant. Comparative studies showed that DBS could make Hb present a more welcome result than SDS since it had an extra benzene ring. On the other hand, since surfactant was a denaturant to Hb as well and the electrode surface might be fouled with it, a negative effect of the surfactant should be considered in the meantime.
Li
G
G
Department of Biochemistry, Nanjing University, P.R. China.
Chen
H
H
Zhu
D
D
D001557:Benzenesulfonates / Q000737:chemistry; D002021:Buffers; D004563:Electrochemistry / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D011489:Protein Denaturation; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry; D014466:Ultraviolet Rays
D001557:Benzenesulfonates; D002021:Buffers; D006454:Hemoglobins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C001114:dodecylbenzenesulfonic acid