title: Fertilization and embryo cleavage after intracytoplasmic spermatid injection in an obstructive azoospermic patient with defective spermiogenesis.
abstract: OBJECTIVE
  To achieve fertilization and cleavage by spermatids without tails from testicular biopsy.
  
  
  METHODS
  Clinical trial.
  
  
  METHODS
  Reproductive unit of a university teaching hospital.
  
  
  METHODS
  A patient of obstructive azoospermia with defective spermiogenesis.
  
  
  METHODS
  Testicular biopsy after scrotal exploration and spermatid injection into the cytoplasm of oocyte.
  
  
  METHODS
  Fertilization and cleavage.
  
  
  RESULTS
  Four of 13 spermatid injections achieved normal fertilization and 2 of them cleaved.
  
  
  CONCLUSIONS
  Intracytoplasmic spermatid injection may be a possible treatment for patients of defective spermiogenesis who have no viable spermatozoa available.
authors: College of Medicine, National Taiwan University, Taipei.
authors: S U
authors: SU
authors: Chen
authors: H N
authors: HN
authors: Ho
authors: H F
authors: HF
authors: Chen
authors: T C
authors: TC
authors: Tsai
authors: T Y
authors: TY
authors: Lee
authors: Y S
authors: YS
authors: Yang
mesh_terms: D000328:Adult; D002970:Cleavage Stage, Ovum; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007267:Injections; D008297:Male; D008846:Micromanipulation; D009845:Oligospermia / Q000503:physiopathology / Q000628:therapy*; D009865:Oocytes; D013087:Spermatids; D013091:Spermatogenesis