[Effect of ovarian cancer produced-transforming growth factor-beta on phytohemagglutinin and interleukin-2-inducing proliferation of peripheral blood mononuclear cells].
OBJECTIVE
Our previous observation has demonstrated that human ovarian cell lines COC1 and COC2 are able to secrete transforming growth factor-beta (TGF-beta) in serum-free culture. The effect of ovarian cancer produced-TGF-beta was observed on phytohemagglutinin and interleukin-2-inducing proliferation of peripheral blood mononuclear cells.


METHODS
The effects of COC1 and COC2 from patients' ascitic fluid (AS1, AS2), the serum-free conditioned medium (SFCM1, SFCM2), and COC1- and COC2- bearing nude mice sera (NS1, NS2) on phytohemagglutinin (PHA) and interleukin-2 (IL-2)-inducing peripheral blood mononuclear cell (PBMC) proliferation and their correlation with TGF-beta were observed and compared.


RESULTS
It was shown that AS, SFCM and NS possessed inhibitory effects on PBMC proliferation. But in very low concentrations, PHA-induced by SFCM2 and IL-2-induced by SFCM1 as well as by NS1 and NS2 exerted promotive effects on PBMC proliferation. The above-mentioned SFCM effects similar to that of TGF-beta, might be partially blocked by anti-TGF-beta antibody.


CONCLUSIONS
The present results suggest that ovarian cancer cells may inhibit the host anti-tumor immunity through secreting TGF-beta, which may be associated with the highly malignant biological behavior of ovarian cancer and the threatening progressiveness of its clinical course.
Xiong
S
S
Nanfang Hospital, First Military Medical University, Guangzhou.
Chen
Y
Y
Wu
A
A
D000818:Animals; D002455:Cell Division; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D016212:Transforming Growth Factor beta