Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata.
Two sulfated xylogalactans (F1 and F7), isolated from the red seaweed Nothogenia fastigiata, achieved a dose-dependent inhibition of the replication of herpes simplex virus type 1 (HSV-1) in Vero cells, with 50% effective doses in the range of 15.0-32.6 micrograms/ml, and without affecting cell viability at concentrations up to 200 micrograms/ml. The presence of sulfate groups in the molecule was essential for the antiviral properties of these polysaccharides. F7 afforded significant inhibition in HSV-1 yield if added to the cell cultures simultaneously with virus inoculum, but had no effect when it was added after 1 h of infection. Analysis of the early events of the viral replicative cycle showed that the anti-HSV effect of F7 was due to a specific inhibition of virus attachment to the host cell whereas virus internalization was not impaired.
Damonte
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EB
Departamento de Química Biológica, Universidad de Buenos Aires, Argentina.
Matulewicz
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MC
Cerezo
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AS
Coto
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CE
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D012624:Seaweed / Q000737:chemistry*; D014709:Vero Cells
D000998:Antiviral Agents; D005685:Galactans