[K562 cells differentiation induced by RNA from rabbit reticulocytes].
OBJECTIVE
To study the effect of RNA from the reticulocytes of rabbit on cell differentiation of human leukemia cell line K562 cells.


METHODS
RNA from rabbit reticulocytes and human leukemia K562 cells were cultured for 4 days to detect cellular changes.


RESULTS
When K562 cells grew in a medium containing 100 micrograms/ml RNA, the cellular growth and division were gradually suppressed and the cells changed toward a differentiated phenotype. Subsequently, K562 cells produced hemoglobins and further differentiated terminally.


CONCLUSIONS
RNA of mammalian erythrocyte played a role in declining cellular division and growth of K562 cells, reversing malignant phenotype, and promoting differentiation.
Zu
S
S
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Gao
C
C
Qiang
L
L
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D012313:RNA / Q000494:pharmacology*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D012313:RNA