Toxicity to transferred rat embryos after aspirin treatment during preimplantation stage in vivo.
OBJECTIVE
To explore the relationship between drug-induced blastopathies and post-implantation embryotoxicity or developmental defects.


METHODS
Pregnant rats on d 3 were given intragastrically aspirin (0.25, 0.5, and 1 g.kg-1). On d 4, the blastocysts were transferred into the uterine horns of pseudopregnant rats (made by mating with male rats which had been given intragastrically 3-chloro-1,2-propanediol 5 mg.kg-1 for 5 d). Uterine contents were examined at term.


RESULTS
The frequency of blastocysts with morphological alterations (FBMA) was increased on d 4 of gestation. The implantation rate was lower than that of the controls. A dose-related increase in resorption (55.2%, 69.5%, and 85.2%) and malformation rate (3.8%, 44.4%, and 25%), and decrease in viability rate of fetuses (44.8%, 30.5%, and 14.8%) were observed in test groups with correlations to FBMA.


CONCLUSIONS
Embryotoxicity and fetal malformations were induced by treatment of aspirin before implantation in a dose-dependent manner.
Lou
Y J
YJ
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Ding
G S
GS
Tu
Z H
ZH
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D001241:Aspirin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects; D020964:Embryo Loss / Q000139:chemically induced*; D004624:Embryo Transfer; D005260:Female; D005333:Fetus / Q000002:abnormalities*; D008297:Male; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens; D001241:Aspirin