title: Pyridoxal 5'-phosphate binds to a lysine residue in the adenosine 3'-phosphate 5'-phosphosulfate recognition site of glycolipid sulfotransferase from human renal cancer cells.
abstract: In the course of characterization of glycolipid sulfotransferase from human renal cancer cells, the manner of inhibition of sulfotransferase activity with pyridoxal 5'-phosphate was investigated. Incubation of a partially purified sulfotransferase preparation with pyridoxal 5'-phosphate followed by reduction with NaBH4 resulted in an irreversible inactivation of the enzyme. When adenosine 3'-phosphate 5'-phosphosulfate was coincubated with pyridoxal 5'-phosphate, the enzyme was protected against this inactivation. Furthermore, pyridoxal 5'-phosphate was found to behave as a competitive inhibitor with respect to adenosine 3'-phosphate 5'-phosphosulfate with a Ki value of 287 microM. These results suggest that pyridoxal 5'-phosphate modified a lysine residue in the adenosine 3'-phosphate 5'-phosphosulfate-recognizing site of the sulfotransferase.
authors: Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
authors: K
authors: K
authors: Kamio
authors: K
authors: K
authors: Honke
authors: A
authors: A
authors: Makita
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000249:Adenosine Monophosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D001665:Binding Sites; D002240:Carbohydrate Sequence; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000201:enzymology*; D008239:Lysine / Q000737:chemistry*; D008969:Molecular Sequence Data; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry*; D011732:Pyridoxal Phosphate / Q000737:chemistry*; D015238:Sulfotransferases / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000249:Adenosine Monophosphate; D010724:Phosphoadenosine Phosphosulfate; D011732:Pyridoxal Phosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase; D008239:Lysine