title: Glycoprotein H (gII/gp108) and glycoprotein L form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1.
abstract: The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by two different panels of monoclonal antibodies. The gII- and gp 108-specific monoclonal antibodies were shown to react with the same protein, which was identified by N-terminal sequencing as the homologue of herpes simplex virus type 1 (HSV-1) gH. When BHV-1 gH was purified by immunoadsorbent chromatography, gL was co-purified. The gH-gL complex induced the production of antibodies that neutralized virus infectivity and inhibited virus penetration. Affinity-purified gH-gL did prevent penetration, but not attachment of BHV-1, which suggests that the gH-gL complex is essential for penetration of BHV-1 into susceptible cells.
authors: Veterinary Infectious Disease Organization, University of Saskatchewa, Saskatoon, Canada.
authors: S
authors: S
authors: van Drunen Littel-van den Hurk
authors: S
authors: S
authors: Khattar
authors: S K
authors: SK
authors: Tikoo
authors: L A
authors: LA
authors: Babiuk
authors: E
authors: E
authors: Baranowski
authors: D
authors: D
authors: Plainchamp
authors: E
authors: E
authors: Thiry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins