Covalent attachment of synthetic DNA to self-assembled monolayer films.
The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.
Chrisey
L A
LA
Naval Research Laboratory, Washington, DC 20375-5348, USA.
Lee
G U
GU
O'Ferrall
C E
CE
D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D006358:Hot Temperature; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D012821:Silanes; D013438:Sulfhydryl Compounds; D012822:Silicon Dioxide; D004247:DNA