Purification and characterization of the staphylococcal slime-associated antigen and its occurrence among Staphylococcus epidermis clinical isolates.
The Staphylococcus epidermidis slime-associated antigen (SAA) was purified and characterized. N-Acetyl-glucosamine accounted for 70% of the dry weight of SAA, which was immunolocalized on the ruthenium red-positive material produced by slime-positive strains. A total of 59% of slime-producing S. epidermidis clinical isolates expressed SAA, while the phenotype slime- SAA+ was never recovered.
Baldassarri
L
L
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
Donnelli
G
G
Gelosia
A
A
Voglino
M C
MC
Simpson
A W
AW
Christensen
G D
GD
D000117:Acetylglucosamine / Q000032:analysis; D000942:Antigens, Bacterial / Q000737:chemistry*; D002451:Cell Compartmentation; D004396:Coloring Agents; D006801:Humans; D016253:Microscopy, Immunoelectron; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012430:Ruthenium Red; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000737:chemistry*
D000942:Antigens, Bacterial; D004396:Coloring Agents; D011135:Polysaccharides, Bacterial; D012430:Ruthenium Red; D000117:Acetylglucosamine