Complete development of Cryptosporidium parvum in MDBK cells.
Sporozoites of Cryptosporidium parvum excysted in vitro from bovine oocysts were incubated with monolayers of Madin-Darby bovine kidney cells. The extent of parasite colonisation was monitored by light microscopy and immunofluorescence. Electron microscopy confirmed the complete development and replication of C. parvum within Madin-Darby bovine kidney cells.
Villacorta
I
I
Laboratory of Parasitology, National Institute of Veterinary Research, Brussels, Belgium.
de Graaf
D
D
Charlier
G
G
Peeters
J E
JE
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D007668:Kidney; D008854:Microscopy, Electron; D010274:Parasitology / Q000379:methods