title: Purification of adult Drosophila melanogaster lipophorin and its role in hydrocarbon transport.
abstract: Lipophorin was isolated from homogenized adult Drosophila melanogaster. It is stained by Sudan Black and has a native molecular mass of 640 kD and a density of 1.12 g/ml. It consists of two glycosylated apoproteins of 240 and 75 kDa. Gas chromatography and mass spectrometry showed that lipophorins isolated separately from virgin 3-day-old male and female flies were associated with specific hydrocarbons, and that these were the same hydrocarbons found in male and female cuticles, respectively. Moreover, a pool of internal hydrocarbons was demonstrated for the first time, with chain lengths similar to those of the cuticular pool. Studies on the fate of the hydrocarbons synthesized de novo after topical applications of radiolabelled fatty acid precursors showed a decrease of the internal pool of hydrocarbons with time, concomitant with an increase of the cuticular pool. These results suggest that hydrocarbons synthesized at an internal site, possibly in oenocytes, may be transported to the cuticle of the flies by lipophorin.
authors: Laboratoire des Mécanismes de Communication, CNRS URA 1491, Université de Paris-Sud, Orsay, France.
authors: D B
authors: DB
authors: Pho
authors: M
authors: M
authors: Pennanec'h
authors: J M
authors: JM
authors: Jallon
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006838:Hydrocarbons / Q000378:metabolism*; D008074:Lipoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male
chemical_list: D002352:Carrier Proteins; D006838:Hydrocarbons; D008074:Lipoproteins; C032830:lipophorin