Induction of apoptosis in a Drosophila neuronal cell line by calcium ionophore.
Apoptotic type cell death was induced by the calcium ionophore, A23187, in a Drosophila CNS derived cell line, ML-DmBG2-c2. It was judged by the ultrastructural observations and internucleosomal DNA fragmentation, typical characteristics of apoptosis. The ionophore failed to induce apoptosis in Ca2+- free medium, which suggests that the increase of intracellular Ca2+ concentration by the treatment of A23187 triggers apoptosis in the clonal cells.
Ui-Tei
K
K
Department of Pharmacology, Nippon Medical School, Tokyo, Japan.
Sato
S
S
Miyake
T
T
Miyata
Y
Y
D000818:Animals; D017209:Apoptosis; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects*; D004247:DNA / Q000097:blood; D004330:Drosophila; D007476:Ionophores; D013997:Time Factors
D007476:Ionophores; D000001:Calcimycin; D004247:DNA; D002118:Calcium