title: Incorporation of bromodeoxyuridine in regenerating fin tissue of the goldfish Carassius auratus.
abstract: We have investigated the pattern of incorporation of 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU) by proliferating cells during regeneration of the tail fin of Carassius auratus. Fifteen days after amputation, intraperitoneal injection of a single dose of 0.25 mg/g wet weight of BrdU and subsequent immunocytochemical detection on sections revealed groups of replicating cells in the blastema and epidermis at different proximodistal levels. Proliferating blastemal cells were confined to a crowded, compact distal area that lost its replicative capacity laterally, causing the differentiation of scleroblasts, which synthesize the lepidotrichia hemisegments. Proximally, but centrally located, the blastemal cells did not incorporate BrdU and they differentiated giving rise to the mature intraray connective tissue. An independent cell-proliferation process was noted in the epidermis. The distal cap did not proliferate; the lateral faces of the epidermis showed high rates of cell replication in the central layer at every level of the regenerate rays; quiescent cells remained in the superficial layers. The basal epidermal cells did not incorporate BrdU when actinotrichia were present. The possible role of basal epidermal cells in the synthesis of actinotrichia, the contribution of these collagen macrofibrils to the morphogenetic process, and the different pathways of cell differentiation during fin regeneration are discussed.
authors: Department of Cell Biology and Genetics, Faculty of Science, University of Málaga, Spain.
authors: J A
authors: JA
authors: Santamaría
authors: M
authors: M
authors: Marí-Beffa
authors: L
authors: L
authors: Santos-Ruiz
authors: J
authors: J
authors: Becerra
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002454:Cell Differentiation; D002455:Cell Division; D004247:DNA / Q000032:analysis; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry; D006054:Goldfish / Q000033:anatomy & histology / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology; D012038:Regeneration / Q000502:physiology*
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine