title: Comparative expression of vasopressin and angiotensin type-1 receptor mRNA in rat hypothalamic nuclei: a double in situ hybridization study.
abstract: Angiotensin II (Ang) injected intracerebroventricularly stimulates neurohypophyseal vasopressin (AVP) release into the peripheral circulation. As we have shown previously, central actions of Ang II in the rat forebrain are mediated by the AT1A receptor subtype. In the present paper, we attempted to clarify the cellular localization of the AT1A receptor mRNA in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in order to reappraise the conflicting data on the nature of the angiotensin II receptor involved in Ang induced vasopressin release. For this purpose, double in situ hybridization was performed using a radioactive AT1A receptor riboprobe and a digoxygenin labeled AVP oligoprobe, and immunohistochemical localization of the glial marker glial fibrillary acidic protein (GFAP) on the same brain slice. The results show neuronal expression of AT1A receptor mRNA mainly in dorsal and medial parvocellular parts of the PVN, its localization in some magnocellular PVN neurons and the absence of its expression in AVP producing neurons either in the PVN or in the SON. Thus, while indirect evidence indicates the involvement of the AT1A receptor subtype in the regulation of CRH and oxytocin release, the stimulation of vasopressinergic neurons is likely due to indirect mechanisms, or to a yet unknown type of angiotensin receptor.
authors: Inserm U36, Collège de France, Paris.
authors: Z
authors: Z
authors: Lenkei
authors: P
authors: P
authors: Corvol
authors: C
authors: C
authors: Llorens-Cortes
mesh_terms: D000818:Animals; D001483:Base Sequence; D003342:Corpus Striatum / Q000737:chemistry*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000235:genetics*; D011954:Receptors, Dopamine / Q000235:genetics*; D013495:Supraoptic Nucleus / Q000378:metabolism; D014158:Transcription, Genetic; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D011954:Receptors, Dopamine; D014667:Vasopressins