[Fas antigen expression on human hematopoietic progenitor cells].
We investigated the expression of Fas(CD95) on hematopoietic progenitor cells. CD34+ cells freshly isolated from bone marrow did not express Fas. However, interferon-gamma(IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) induced the expression of Fas after 48 hours of serum-free culture. The TNF-alpha-induced Fas expression is mediated by p55-TNF-alpha receptor. Human CD34+ cells expressed Fas following low dose ionizing radiation in a dose-dependent fashion. CD34+ cells isolated from bone marrow were cultured with hematopoietic growth factors for 7 days. CD34+ cells cultured with hematopoietic growth factors gradually became positive for Fas and rapidly lost Bcl-2 expression. Fas system is considered to play important roles at the level of hematopoietic progenitor cells in both physiologic and pathologic conditions.
Nagafuji
K
K
Dep. of Hematology, Hara Sanshin General Hospital.
Takenaka
K
K
Niho
Y
Y
D000818:Animals; D018952:Antigens, CD34; D017209:Apoptosis; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D016207:Cytokines; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D019014:fas Receptor / Q000378:metabolism* / Q000502:physiology
D018952:Antigens, CD34; D016207:Cytokines; D019014:fas Receptor