title: Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum.
abstract: Two lectins, TML-1 and TML-2, were isolated from Tricholoma mongolicum by ion-exchange chromatography and gel filtration. They are dimers with molecular weight near 37000. The hemagglutinating activities of TML-1 and TML-2 are sensitive to lactose inhibition and are stable between 10 and 80 degrees C. They exhibit antiproliferative activities against mouse monocyte-macrophage PU5-1.8 cells and mouse mastocytoma P815 cells in vitro. The two lectins differ in the content of proline and tyrosine residues. Both are non-glycoproteins and have hydroxyproline residues.
authors: Department of Biology, Chinese University of Hong Kong, New Territories, Hong Kong.
authors: H X
authors: HX
authors: Wang
authors: T B
authors: TB
authors: Ng
authors: W K
authors: WK
authors: Liu
authors: V E
authors: VE
authors: Ooi
authors: S T
authors: ST
authors: Chang
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D037102:Lectins