Sex determination in infants with ambiguous genitalia using the polymerase chain reaction of an X-Y homologous region.
A polymerase chain reaction (PCR) technique applicable for sex identification is described. The technique amplifies a part of the X-Y homologous amelogenin gene in which the Y counterpart has a 189 bp deletion within one of the introns. To evaluate the value of PCR of a X-Y homologous region in sex determination, individuals with ambiguous genitalia were selected for study. By identification of the X and Y sequences based on the difference in their sizes, sex determination could be successfully made.
Tsai
C H
CH
Department of Pediatrics, China Medical College Hospital, Taichung, Taiwan, R.O.C.
Tsai
F J
FJ
Hwu
W L
WL
Wang
T R
TR
Shu
S G
SG
Liu
S C
SC
D001483:Base Sequence; D005260:Female; D005835:Genitalia / Q000002:abnormalities*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*