Larval salivary gland proteins of the sheep nasal bot fly, (Oestrus ovis L.), are major immunogens in infested sheep.
Tissue extracts from larval instars of the sheep nasal bot, Oestrus ovis, were resolved by gel electrophoresis under both native and denaturing conditions. Polypeptides resolved under these conditions were tested by immunoblotting against sera of infested sheep. Of all tissues examined in this study, salivary glands proved to be major immunogens in infested sheep. Salivary gland polypeptides were also detected in the washing solution as larval secretory products (LSP). To a minor extent, a few polypeptides from the larval cuticle were also found to be immunogenic, but they did not contribute to LSP. These results were further corroborated by nasal infestation of rabbits that also developed specific antibodies against larval salivary gland polypeptides from Oestrus ovis.
Innocenti
L
L
Department of Veterinary Medicine, University of Pisa, Italy.
Masetti
M
M
Macchioni
G
G
Giorgi
F
F
D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D004175:Diptera / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007814:Larva; D009198:Myiasis / Q000276:immunology / Q000662:veterinary*; D009666:Nose; D011817:Rabbits / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000276:immunology* / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases
D000941:Antigens; D012471:Salivary Proteins and Peptides