[Effects of insulin and captopril on growth of glomerular mesangial cells in STZ-induced diabetic rats].
OBJECTIVE
To observe the effects of insulin and captopril on growth of mesangial cells and on production of interleukin-6 (IL-6) and tumor necrosis factor (TNF) in culture media of glomerular cells.


METHODS
Streptozocin induced diabetic rats (DM rats) were compared with normal wistar rats.


RESULTS
The CPM value of 3H-TdR incorporated into mesangial cells in DM rats was significantly higher than that in Wistar rats. When the culture media was added by 5 x 10(-4) U/ml, 5 x 10(-3) U/ml and 5 x 10(-1) U/ml of insulin, the CPM value incorporated into mesangial cells and the concentration of IL-6 in the culture media of both DM and wistar rats were significantly higher than those of the control (no insulin). When captopril (10(-6) mol/L, 10(-4) mol/L, 10(-3) mol/L) was added into the media in different concentration, the cpm value of 3H-TdR and the concentration of TNF in DM rats were significantly lower than those of the control (no captopril). But the CPM value of 3H-TdR in Wistar rats was lower than that in the control, which was only seen in the concentration of 10(-3)M captopril.


CONCLUSIONS
Ins could promote 3H-TdR in corporation and increase the production of IL-6, and captopril inhibited 3H-TdR in corporation and decrease the production of TNF.
Geng
J
J
Department of Endocrinology, Chinese PLA General Hospital, Beijing.
Lu
J
J
Pan
C
C
D000818:Animals; D002216:Captopril / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005920:Glomerular Mesangium / Q000473:pathology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007004:Hypoglycemic Agents; D007328:Insulin; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin; D002216:Captopril