Trifluoperazine reduces the expression of CD69 in phytohemagglutinin-activated lymphocytes.
Trifluoperazine (TFP) is a phenothiazine capable of inhibiting lymphocyte proliferation as well as natural killer cells (NK) and lymphokine-activated killer cells (LAK) cytotoxic activity. CD69 is a surface molecule induced by various mechanisms of cellular activation. In the present work the modulation of CD69 expression by TFP was investigated on PHA-stimulated peripheral blood mononuclear cells and compared to that of CD25 (IL-2 receptor) expression. Determination of surface molecules was performed in an indirect immunofluorescence assay using anti-CD69 or anti-CD25 monoclonal antibodies, and analyzed by flow cytometry. The time course of the expression of these two molecules differed: CD69 expression was already declining at 48 h, whereas CD25 was still increasing at 72 h after stimulation. TFP (10 microM) reduced CD69 expression by 71.8% at 24 h, 68.4% at 48 h and 24% at 72 h following activation. In contrast, the same dose of TFP did not significantly affect CD25 expression at 24 h but showed an inhibitory effect at later times. These results suggest that different activation pathways are involved in the expression of CD25 and CD69.
Pires
V
V
Departamento de Patologia, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Harab
R C
RC
Rumjanek
V M
VM
D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D006801:Humans; D037181:Lectins, C-Type; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins; D014268:Trifluoperazine / Q000494:pharmacology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D010835:Phytohemagglutinins; D014268:Trifluoperazine; D000099192:CD69 Antigens