Multiplex amplification and typing procedure for the loci D1S80 and amelogenin.
A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 and amelogenin using discontinuous polyacrylamide gel electrophoresis and silver staining. The protocol is sensitive, simple, rapid, and relatively inexpensive. The results of the multiplex analysis of the D1S80 and amelogenin loci were comparable to those obtained when each locus was analyzed individually. A small validation study was undertaken to evaluate the forensic applicability of this multiplex system. The data demonstrate that DNA exposed to a variety of environmental insults yields reliable multiplex typing results.
Budowle
B
B
Forensic Science Research Unit, FBI Laboratory, FBI Academy, Quantico, Virginia 22135, USA.
Koons
B W
BW
Errera
J D
JD
D053523:Amelogenin; D000818:Animals; D001801:Blood Stains; D001826:Body Fluids / Q000737:chemistry; D002878:Chromosomes, Human, Pair 1; D004247:DNA / Q000032:analysis*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D012661:Semen / Q000737:chemistry; D012680:Sensitivity and Specificity; D013045:Species Specificity
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA