Prostate specific antigen--a new constituent of breast cyst fluid.
We demonstrate for the first time that prostate specific antigen (PSA) is a component of breast cyst fluid. At the cutoff level of 0.01 or 0.03 micrograms/L of PSA, 64% or 43% of cyst fluids are positive for PSA, respectively. PSA in cyst fluid, as characterized by high performance liquid chromatography, exists in almost equal concentrations as free PSA, with a molecular weight of 33 KDa, and as PSA bound to alpha 1-antichymotrypsin, with a molecular weight of 100 KDa. PSA presence was also characterized in cyst fluid by Western blot analysis. These data suggest that PSA is a frequent component of breast cyst fluid. More studies are needed to establish the role of this serine protease in normal breast, gross breast cystic disease, and breast cancer.
Diamandis
E P
EP
Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada.
Yu
H
H
López-Otín
C
C
D000328:Adult; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D005122:Exudates and Transudates / Q000378:metabolism; D005260:Female; D005348:Fibrocystic Breast Disease / Q000378:metabolism*; D006801:Humans; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000737:chemistry
D017430:Prostate-Specific Antigen