title: A topographic study on the distribution of cisplatin in xenografted tumors on nude mice.
abstract: The intratumoral distribution of cisplatin was studied in terms of platinum concentrations in tissue pieces and immunohistochemically detected cisplatin-DNA adducts in xenografted tumors on nude mice. Heterogeneities in drug distribution were calculated as standard deviations and coefficients of variation. A three-dimensional image of adduct distribution was produced which showed regions with low adduct levels to be topographically connected also in three dimensions. A model was presented for investigating the potential influence of vascularization and cell proliferation on intratumoral adduct distribution by using different immunohistochemical stainings of parallel tissue sections. A weak but significant correlation was found between cisplatin-DNA adducts and proliferation, which might indicate that the drug uptake and adduct formation is increased in proliferating cells.
authors: Department of Oncology, University Hospital, Lund, Sweden.
authors: A
authors: A
authors: Johnsson
authors: E
authors: E
authors: Cavallin-Stahl
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000737:chemistry*; D002945:Cisplatin / Q000032:analysis*; D018736:DNA Adducts / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010984:Platinum / Q000032:analysis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D010984:Platinum; D002945:Cisplatin