DNA single and double strand breaks induced by aliphatic and aromatic aldehydes in combination with copper (II).
The aliphatic n-butyr-and n-valeraldehyde as well as the aromatic benz- and anisaldehyde induced DNA strand breaks in PM2 DNA in the presence of CuCl2. Neither aldehydes nor CuCl2 alone showed DNA breakage properties. The maximum of single strand breaks (SSBs) induced by the combination of CuCl2 and aldehydes was dependent on the CuCl2-concentration. The aliphatic aldehydes induced SSBs and double strand breaks (DSBs) at lower concentrations than aromatic aldehydes when optimal CuCl2 concentration were used. Catalase and neocuproine nearly completely inhibited strand break formation induced by aromatic aldehydes/CuCl2. The prevention of strand breaks induced by aliphatic aldehydes/CuCl2 was less effective. While the inhibition by neocuproine was only 25%, catalase was totally ineffective. In all aldehydes/CuCl2 mixtures the formation of Cu(I) was observed. The results point to different DNA damaging species produced during redox reactions of aromatic and aliphatic aldehydes in combination with CuCl2.
Becker
T W
TW
Carl-von-Ossietzky Universität Oldenburg, ICMB/FB7, F.R.G
Krieger
G
G
Witte
I
I
D000447:Aldehydes / Q000633:toxicity*; D001547:Benzaldehydes / Q000633:toxicity*; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects*; D015394:Molecular Structure; D009153:Mutagens / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000447:Aldehydes; D001547:Benzaldehydes; D004279:DNA, Viral; D009153:Mutagens; D017382:Reactive Oxygen Species; D003300:Copper; C024896:4-anisaldehyde; C046012:pentanal; C018475:butyraldehyde; C029892:cupric chloride; C032175:benzaldehyde