The lactate dehydrogenase gene from nematode Caenorhabditis elegans contains only two of six introns conserved in the protein-encoding sequence of LDH genes from bird and mammals.
The protein-encoding region of L-lactate dehydrogenase (LDH) gene from nematode, Caenorhabditis elegans, was amplified by polymerase-chain-reaction from total genomic DNA and its nucleotide sequence determined. A comparison of this genomic sequence with the published sequence of nematode LDH cDNA reveals the presence of two introns of 57 and 47 nucleotides at codon no. 82 and 279-280, respectively. The positions of the two introns present in this invertebrate LDH gene correspond to the second and sixth introns of vertebrate LDH genes. The protein-coding sequence of human LDH-A (muscle), LDH-B (heart) and LDH-C (testis), mouse LDH-A, and duck LDH-B genes has previously been shown to be interrupted by six introns at the homologous positions.
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Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D017124:Conserved Sequence; D004372:Ducks / Q000235:genetics*; D017238:Genes, Helminth; D005815:Genetic Code; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D007438:Introns; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data
D015801:Helminth Proteins; D007770:L-Lactate Dehydrogenase