A competitive prehybridisation assay for validating the specificity of oligonucleotide probes: application for analysis of rat metallothionein -1 and -2 mRNA.
Vasconcelos
M H
MH
Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen, UK.
Tam
S C
SC
Beattie
J H
JH
Hesketh
J E
JE
D000255:Adenosine Triphosphate; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001483:Base Sequence; D008668:Metallothionein / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D015203:Reproducibility of Results
D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D008668:Metallothionein