Sphingosine, oleylamine and stearylamine inhibit both CD11a/CD18-dependent and -independent homotypic aggregation: demonstration by cytofluorimetry.
An CD11a/CD18-dependent homotypic aggregation pathway is induced by triggering CD45 molecules on human thymocytes. By contrast, a CD11a/CD18-independent homotypic aggregation process is induced by triggering the CD99 molecule (E2, MIC2 gene product) expressed at the surface of either Jurkat T cells or human thymocytes. A new quantitative method based on FACS analysis of aggregated cells was used and allowed to show that both types of aggregation (CD11a/CD18-dependent and CD11a/CD18-independent) were inhibited with sphingosine, oleylamine or stearylamine. These three compounds had no effect on the expression of CD99, CD45, CD11a, CD18 or other [correction of others] known integrins expressed at the surface of the cells studied.
Aussel
C
C
INSERM U343, Hôpital de l'Archet, BP. 79, 06202 Nice, France.
Mahmoudi
A H
AH
Bernard
G
G
Breittmayer
J P
JP
Bernard
A
A
D000071443:12E7 Antigen; D000588:Amines / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002449:Cell Aggregation / Q000276:immunology*; D002460:Cell Line; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016023:Integrins / Q000096:biosynthesis; D007700:Kinetics; D017493:Leukocyte Common Antigens / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D013110:Sphingosine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000071443:12E7 Antigen; D000588:Amines; D015703:Antigens, CD; D018821:CD18 Antigens; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D017493:Leukocyte Common Antigens; C009317:stearylamine; D013110:Sphingosine; C008703:oleylamine