Catecholamines decrease lymphocyte adhesion to cytokine-activated endothelial cells.
Numerous studies have shown that catecholamines can modulate lymphocyte migration. This effect may be mediated in part by modulation of lymphocyte-endothelial cell interactions, which is dependent on adhesion molecules expressed on both of these cells. Our results show that catecholamines decreased T-cell binding to IL-1 activated endothelial cells in vitro. The decrease in adhesion was not mediated by a change in adhesion molecule expression as LFA-1 and VLA-4 expression on T-cells and ICAM-1 and VCAM-1 expression on endothelial cells were not changed by catecholamine stimulation. T-cells flatten and enlarge the area of surface contact as they adhere to endothelial cells. Image analysis of the number of T-cells bound and the amount of cell spreading over several time points suggests that catecholamines alter the kinetics of T-cell-endothelial cell adhesion. These results support the hypothesis that catecholamines can alter lymphocyte-endothelial interactions in vivo, which in turn would affect lymphocyte migration.
Carlson
S L
SL
Department of Anatomy and Neurobiology, University of Kentucky Medical Center, Lexington 40536-0084, USA.
Beiting
D J
DJ
Kiani
C A
CA
Abell
K M
KM
McGillis
J P
JP
D002395:Catecholamines / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D016207:Cytokines / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D011247:Pregnancy; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
D002395:Catecholamines; D015815:Cell Adhesion Molecules; D016207:Cytokines; D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein