Sound induced expression of c-Fos in GABA positive neurones of the gerbil cochlear nucleus.
To characterize c-Fos-expressing neurones in more detail, a double labelling technique was developed to simultaneously demonstrate the presence of GABA-like and c-Fos-like immunoreactivity in cryostat sections of the gerbil cochlear nucleus. The animals were exposed to a wide-band noise stimulus for the induction of c-Fos expression. The distribution of c-Fos-positive cells and GABA-positive somata was consistent with previous reports. Our double labelling approach revealed the presence of GABA-positive cells that showed c-Fos expression after noise stimulation. One interpretation of this finding is that a prolonged acoustic exposure induces long term changes in inhibitory interneurones to adjust the output of the cochlear nucleus.
Gleich
O
O
ENT Department, University of Regensburg, Germany.
Bielenberg
K
K
Strutz
J
J
D000161:Acoustic Stimulation; D000818:Animals; D017626:Cochlear Nucleus / Q000166:cytology / Q000378:metabolism*; D005849:Gerbillinae; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism*; D009622:Noise; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D005680:gamma-Aminobutyric Acid