Determination of the potent antiprotozoal compound atovaquone in plasma using liquid-liquid extraction followed by reversed-phase high-performance liquid chromatography with ultraviolet detection.
A specific and robust method is presented for the determination of atovaquone in plasma. Atovaquone is a potent antiprotozoal compound for use in immunocompromised patients who are intolerant of conventional therapies. The method involves a liquid-liquid extraction of the compound into hexane modified with 2% (v/v) isoamyl alcohol. The processed extracts are analysed by reversed-phase high-performance liquid chromatography with ultraviolet detection at 254 nm. The assay has a limit of quantification of 0.1 microgram/ml and is linear between 0.1 and 50 micrograms/ml. The method has been applied to many clinical studies and has been demonstrated to be precise and accurate with high sample throughput. Atovaquone is not significantly metabolised in humans.
Hannan
S L
SL
Wellcome Research Laboratories, Beckenham, Kent, UK.
Ridout
G A
GA
Jones
A E
AE
D000981:Antiprotozoal Agents / Q000097:blood*; D053626:Atovaquone; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D006801:Humans; D008297:Male; D009285:Naphthoquinones / Q000097:blood*; D012680:Sensitivity and Specificity
D000981:Antiprotozoal Agents; D009285:Naphthoquinones; D053626:Atovaquone