title: Immunolocalizations of human gelatinase (type IV collagenase, MMP-9) and TIMP (tissue inhibitor of metalloproteinases) in normal epidermis and some epidermal tumors.
abstract: The matrix metalloproteinases (MMPs) MMP-2 and MMP-9 (gelatinases) have been suggested as serving an important role in cleaving the basement membrane structure. Tissue inhibitors of metalloproteinases TIMPs (particularly TIMP-1) are known to inhibit MMPs. Based on this background, we raised monoclonal antibodies against human gelatinase (MMP-9) and human recombinant TIMP (TIMP-1), and immunostained these two components in skin from patients with squamous cell carcinoma (SCC), Bowen's disease (BD) and keratoacanthoma (KA). MMP-9 showed positive staining mainly in the granular layer of normal epidermis. In some cases of SCC and BD, MMP-9 showed positive staining in the dysplastic lesions even in the basal layer. TIMP showed a thorough positivity in normal epidermis. Unstained regions with this antibody were observed in SCC and BD. These results suggest that an altered staining pattern for MMP-9 and TIMP may be closely related to the malignant transformation of SCC and BD.
authors: Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
authors: T
authors: T
authors: Kobayashi
authors: N
authors: N
authors: Onoda
authors: T
authors: T
authors: Takagi
authors: H
authors: H
authors: Hori
authors: S
authors: S
authors: Hattori
authors: Y
authors: Y
authors: Nagai
authors: S
authors: S
authors: Tajima
authors: T
authors: T
authors: Nishikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D001913:Bowen's Disease / Q000201:enzymology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D017364:Collagenases / Q000378:metabolism*; D004817:Epidermis / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007636:Keratoacanthoma / Q000201:enzymology; D008297:Male; D020780:Matrix Metalloproteinase 9; D051379:Mice; D008807:Mice, Inbred BALB C; D011480:Protease Inhibitors / Q000378:metabolism; D012016:Reference Values; D012878:Skin Neoplasms / Q000208:ethnology*; D014018:Tissue Distribution; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases; D020780:Matrix Metalloproteinase 9