Coinfection of a bearded dragon, Pogona vitticeps, with adenovirus- and dependovirus-like viruses.
Four neonate bearded dragons, Pogona vitticeps, from two collections became ill and died. Multiple tissues were collected and processed for light microscopy. In hematoxylin and eosin-stained sections of liver of one lizard, numerous basophilic intranuclear inclusions were observed. In three lizards, intranuclear inclusions were primarily seen within enterocytes in the small intestine. A portion of paraffin-embedded liver of one lizard and small intestine of a second lizard were removed, deparaffinized, and examined by electron microscopy. For the most part, inclusions in the liver consisted of nonenveloped viral particles 60-66 nm in diameter. Smaller nonenveloped virions 15-17 nm in diameter were occasionally seen in association with these particles. In the intestine, inclusions consisted only of 60-70 nm particles. Based on morphology and location, the larger particles were consistent with an adenovirus. Based on size and presence within nuclei of host cells coinfected with the adenovirus-like virus, the smaller viral agent was consistent with members of the genus Dependovirus.
Jacobson
E R
ER
University of Florida, Gainesville 32610, USA.
Kopit
W
W
Kennedy
F A
FA
Funk
R S
RS
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D000229:Dependovirus / Q000302:isolation & purification*; D007421:Intestine, Small / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D008099:Liver / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D008116:Lizards / Q000821:virology*; D010322:Parvoviridae Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D014771:Virion / Q000648:ultrastructure