Preparation of apolactoferrin with a very low iron saturation.
Iron(III) removal from lactoferrin by an iron(III)-chelating resin with immobilized 3-hydroxy-2-methyl-4(1H)-pyridinone ligands was studied at physiological pH in the presence of citrate. The resin had a marked effect on the extent of iron removal. By using the iron(III)-chelating resin, removal of iron from lactoferrin was nearly complete in < 24 h. Apolactoferrin with 4% iron saturation could be prepared conveniently from 100% or from 18% iron-saturated lactoferrin under mild conditions without affecting the iron-binding capacity of the protein. The iron saturation of the obtained apolactoferrin was much lower than that of the apolactoferrin prepared by reported methods.
Feng
M
M
Department of Chemical Technology, Biomaterials Section, University of Twente, Enschede, The Netherlands.
van der Does
L
L
Bantjes
A
A
D001059:Apoproteins / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002951:Citrates; D019343:Citric Acid; D005290:Ferric Compounds / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007502:Iron Chelating Agents; D007781:Lactoferrin / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
D001059:Apoproteins; D002951:Citrates; D005290:Ferric Compounds; D007502:Iron Chelating Agents; C039223:apolactoferrin; D019343:Citric Acid; D007781:Lactoferrin