Future prospects for the analysis of complex biological systems using micro-column liquid chromatography-electrospray tandem mass spectrometry.
An overview is provided of methods for the study of complex biological processes by using micro-column liquid chromatography-electrospray ionization tandem mass spectrometry. Procedures discussed include electrospray ionization, micro-column liquid chromatography, tandem mass spectrometry, tandem mass spectra data interpretation for peptides, and database searching with mass spectral data. Several problems in immunology are discussed to illustrate this approach.
Yates
J R
JR
Department of Molecular Biotechnology, School of Medicine, University of Washington, Seattle 98195-7730, USA. jyates@u.washington.edu
McCormack
A L
AL
Link
A J
AJ
Schieltz
D
D
Eng
J
J
Hays
L
L
D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D008967:Molecular Biology / Q000295:instrumentation* / Q000639:trends; D008969:Molecular Sequence Data