Identification of retinolisomers by high-performance liquid chromatography not unequivocal up to now.
Former publications dealing with high-performance liquid chromatography (HPLC) of retinol provide inconsistent results and lack evidence for the identification of isomers. For clarification of the problems in this field, the isomeric structure of the major retinol isomers in crystallized form was proven by 1H-NMR, UV absorption spectroscopy and by X-ray analysis. These identified isomeric forms of retinol were separated by HPLC with different eluent mixtures and the chromatograms compared in detail with previously published data. A conclusive assignment for HPLC separation of retinol isomers is given.
Nöll
G N
GN
Kalinowski
H O
HO
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000737:chemistry*
D014801:Vitamin A