Interleukin-3-associated ganglioside GD1a is induced independently of normal interleukin-3 receptor in murine myelogenous leukaemia NFS60 cells transfected with the interleukin-3 gene.
The mechanism of interleukin-3 (IL-3) independent cell growth and of IL-3-associated ganglioside expression was analysed using the IL-3 dependent murine myelogenous leukaemia cell line NFS60-I7 and IL-3 gene-transfected sublines. The transfected cell lines showed autonomous cell growth, tumorigenicity, and IL-3 associated ganglioside GD1a expression in spite of their IL-3 production. While the parental NFS60-I7 cells did not express significant amounts of GD1a, exogenous recombinant IL-3 (rIL-3) was demonstrated to induce IL-3-associated ganglioside GD1a expression in NFS60-I7 cells. Furthermore, the growth potential of the transfected cells was not blocked by anti-IL-3 antibody and expression of GD1a was not affected by anti-IL-3 antibody. These findings suggest that IL-3 expressed intracellularly by gene transfection might act independently of the normal IL-3 receptor on autonomous cell growth and on IL-3-associated GD1a expression in murine myelogenous leukaemia NFS60 cells.
Nakamura
M
M
Division of Hemopoiesis, Jichi Medical School, Minamikawachi, Tochigi, Japan.
Kirito
K
K
Tsunoda
A
A
Hara
K
K
Furukawa
Y
Y
Saito
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005732:Gangliosides / Q000096:biosynthesis*; D007377:Interleukin-3 / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured
D005732:Gangliosides; D007377:Interleukin-3; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins; C021298:ganglioside, GD1a