Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum.
Two lectins, TML-1 and TML-2, were isolated from Tricholoma mongolicum by ion-exchange chromatography and gel filtration. They are dimers with molecular weight near 37000. The hemagglutinating activities of TML-1 and TML-2 are sensitive to lactose inhibition and are stable between 10 and 80 degrees C. They exhibit antiproliferative activities against mouse monocyte-macrophage PU5-1.8 cells and mouse mastocytoma P815 cells in vitro. The two lectins differ in the content of proline and tyrosine residues. Both are non-glycoproteins and have hydroxyproline residues.
Wang
H X
HX
Department of Biology, Chinese University of Hong Kong, New Territories, Hong Kong.
Ng
T B
TB
Liu
W K
WK
Ooi
V E
VE
Chang
S T
ST
D001487:Basidiomycota / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology
D037102:Lectins