title: Method for the isolation of biologically active monomeric immunoglobulin A from a plasma fraction.
abstract: A purification method for immunoglobulin A (IgA) yielding monomeric IgA with a purity of over 97% has been developed. This procedure uses ethanol-precipitated plasma (Cohn fraction III precipitate) as the starting material and includes heparin-Sepharose adsorption, dextran sulfate and ammonium sulfate precipitation, hydroxyapatite chromatography, batch adsorption by an anion-exchange matrix and gel permeation. Additional protein G Sepharose treatment leads to an IgA preparation of greater than 99% purity. The isolated IgA presented with an IgA subclass distribution, equivalent to IgA in unfractionated plasma, and was biologically active, as was shown by its ability to down-modulate Haemophilus influenzae-b-induced IL-6 secretion of human monocytes.
authors: Immuno AG, Department of Immunological Research, Vienna, Austria.
authors: H
authors: H
authors: Leibl
authors: R
authors: R
authors: Tomasits
authors: H M
authors: HM
authors: Wolf
authors: M M
authors: MM
authors: Eibl
authors: J W
authors: JW
authors: Mannhalter
mesh_terms: D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D006193:Haemophilus influenzae; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D015850:Interleukin-6 / Q000378:metabolism; D046911:Macromolecular Substances; D009000:Monocytes / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D015850:Interleukin-6; D046911:Macromolecular Substances; D000431:Ethanol; D017886:Durapatite