title: Death and replacement of uterine epithelial cells during oil-induced deciduoma development in the mouse.
abstract: BACKGROUND
  A decidual cell reaction can be induced in rodent endometrium by an intrauterine injection of oil. The epithelial lining is thought to be instrumental to transduce intralumenal stimuli for decidualization. One of the consequences of oil injection is the death of uterine epithelial cells. No information is available on the effect that sustained contact with oil has on the epithelium.
  
  
  METHODS
  A decidual cell reaction was induced in 4-day pseudopregnant mice by injection of 30 microliters of arachis oil into the uterine lumen. Samples from the uteri were collected 24, 48, and 72 h after the injection and prepared for transmission electron microscopy.
  
  
  RESULTS
  Twenty-four hours after the oil injection, some of the initial modifications of epithelial cell surfaces were very similar to those induced by the contact with the blastocyst during normal pregnancy. Uterine epithelial cells internalized injected oil and many cells were seen in various stages of degeneration. At 48 h, many epithelial cells were detached from the basal lamina. At 72 h, the uterine lining was re-established by flattened cells.
  
  
  CONCLUSIONS
  The contact of oil with the uterine epithelium of pseudo pregnant mice induces epithelial cell death in the antimesometrial region of the uterine crypt. There is, however, replacement of epithelial lining by epithelial cells, which probably migrate from the mesometrial region of the crypt. The prolonged presence of oil within the uterine lumen seems to induce cycles of epithelial cell death and replacement.
authors: Department of Histology and Embryology, University of São Paulo, Brazil.
authors: C G
authors: CG
authors: Andrade
authors: P A
authors: PA
authors: Abrahamsohn
authors: F
authors: F
authors: Godinho
authors: E
authors: E
authors: Samuel
authors: T M
authors: TM
authors: Zorn
mesh_terms: D000818:Animals; D016923:Cell Death; D002455:Cell Division; D003656:Decidua / Q000254:growth & development*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D051379:Mice; D008854:Microscopy, Electron; D000074241:Peanut Oil; D010938:Plant Oils / Q000008:administration & dosage; D011247:Pregnancy; D013997:Time Factors
chemical_list: D000074241:Peanut Oil; D010938:Plant Oils