A primer extension method for quantitation of in vitro synthesized mRNA transcripts directed by promoter elements containing multiple transcription initiation sites.
Kosovsky
M J
MJ
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA. M.Kosovsky@UCHSC.edu
Johannes
G
G
D001483:Base Sequence; D003848:Deoxyguanine Nucleotides; D054306:Dideoxynucleotides; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic
D003848:Deoxyguanine Nucleotides; D054306:Dideoxynucleotides; D012333:RNA, Messenger; C031709:2',3'-dideoxyguanosine 5'-triphosphate; D008156:Luciferases