Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia.
A nested PCR which amplified a portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was used to detect P. carinii DNA in blood from patients with P. carinii pneumonia. P. carinii DNA was not detected in serum and was detected at low levels of blood cells.
Tamburrini
E
E
Department of Infectious Diseases, Università Cattolica del S. Cuore, Rome, Italy.
Mencarini
P
P
Visconti
E
E
Zolfo
M
M
De Luca
A
A
Siracusano
A
A
Ortona
E
E
Wakefield
A E
AE
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000097:blood* / Q000235:genetics*; D005188:False Negative Reactions; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D009504:Neutrophils / Q000382:microbiology; D011010:Pneumocystis / Q000235:genetics* / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004271:DNA, Fungal