Specific monoclonal antibodies against leukocyte-restricted cell surface molecule CD43 react with nonhematopoietic tumor cells.
CD43 is the major sialoglycoprotein found on the surface of cells of hemopoietic origin. Because CD43 is a typical member of the surface mucins, molecules that play a relevant role in tumor progression, we have addressed the expression of CD43 in nonhematopoietic tumor cells. For this purpose, we have tested a broad panel of anti-CD43 antibodies on tumors of different embryonic origins. Of the 17 anti-CD43 mAbs used, 9 reacted with leukocytes in paraffin-fixed formalin-embedded lymph node tissues. Two mAbs, BS1 and L10, stained 24 out of 31 (78%) nonhematopoietic tumors analyzed; ie., some tumors were positive for both antibodies, and others were positive for either L10 or BSL We have also found reactivity of these two anti-CD43 mAbs in 13 out of 20 metastases studied.
Santamaría
M
M
Unidad de Immunología, Hospital Universitario Reina Sofia, Córdoba, Spain.
López-Beltrán
A
A
Toro
M
M
Peña
J
J
Molina
I J
IJ
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015703:Antigens, CD; D005557:Formaldehyde; D006801:Humans; D051917:Leukosialin; D008198:Lymph Nodes / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry*; D009439:Neuraminidase / Q000494:pharmacology; D016612:Paraffin Embedding; D012795:Sialoglycoproteins / Q000276:immunology*; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D005557:Formaldehyde; D009439:Neuraminidase