Flow-injection spectrophotometric determination of adrenaline and dopamine with sodium hydroxide.
A new, rapid and economical flow-injection method for determining adrenaline and dopamine is proposed on the basis of the hydrolysis of these compounds in alkaline medium. The method was optimized by using a spectrophotometer operating at lambda = 390 nm as detector. Calibration graphs were linear up to 2 x 10(-4) M with quantification limits of 2.5 x 10(-6) M and 3.3 x 10(-6) M for dopamine and adrenaline respectively. Flow-injection allows the measurement of 130 samples per hour. The method was successfully applied for the determination of catecholamines in pharmaceuticals.
Berzas Nevado
J J
JJ
Department of Analytical Chemistry and Food Technology, Universidad de Castilla-La Mancha, Ciudad Real, Spain.
Lemus Gallego
J M
JM
Buitrago Laguna
P
P
D002626:Chemistry, Pharmaceutical; D004298:Dopamine / Q000032:analysis*; D004837:Epinephrine / Q000032:analysis*; D017022:Flow Injection Analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012044:Regression Analysis; D012972:Sodium Hydroxide; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods
D012996:Solutions; D012972:Sodium Hydroxide; D004298:Dopamine; D004837:Epinephrine