Detection method for polygalacturonase-producing strains of Saccharomyces cerevisiae.
In the presence of glycerol or ethanol, SCPP (a strain of Saccharomyces cerevisiae that expresses pectinolytic activity) is capable of utilizing galacturonic acid or pectins for growth purposes. We now establish a relationship between the pectinolytic power of various strains of S. cerevisiae and their ability to grow on a pectin/glycerol-based medium. This property is further exploited for the detection of polygalacturonase-producing strains of S. cerevisiae.
Gainvors
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Université de Reims, Faculté des Sciences, Laboratoire de Microbiologie Générale et Moléculaire, France.
Belarbi
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D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D013045:Species Specificity
D003470:Culture Media; D006603:Hexuronic Acids; D000431:Ethanol; C007819:galacturonic acid; D010368:Pectins; D011096:Polygalacturonase; D005990:Glycerol