Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR.
Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.
Turkoglu
S
S
Service de Bactériologie-Virologie, Hôpital Antoine Béclère, Paris, France.
Lazizi
Y
Y
Meng
H
H
Kordosi
A
A
Dubreuil
P
P
Crescenzo
B
B
Benjelloun
S
S
Nordmann
P
P
Pillot
J
J
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D016751:Hepatitis E / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008252:Macaca fascicularis; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood / Q000235:genetics*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D017931:DNA Primers; D012367:RNA, Viral