title: Lack of association of secretory component with IgA in J chain-deficient mice.
abstract: J chain has been proposed to play a role in the mucosal transport of polymeric Igs (pIg) by the polymeric Ig receptor (pIgR). We have previously reported the generation of J chain-deficient mice. These mice exhibited elevated serum IgA and depleted biliary and fecal IgA levels compared with wild-type mice. We report here that, unlike the IgA levels in bile and feces, IgA levels in local mucosal and glandular secretions were not depressed in J chain-deficient mice. Breast milk, intestinal mucosal surface, and nasal wash IgA levels in the mutant mice were similar to wild-type mice while bronchoalveolar lavage IgA levels were higher in the J chain-deficient animals. Western blot analysis with an Ab to secretory component (SC), the portion of the pIgR that remains bound to pig in secretions, and immunoprecipitation with Abs directed against IgA showed that secreted IgA was associated with SC in wild-type but not J chain-deficient mice. The IgA in wild-type secretions was polymeric while the secretions of J chain-deficient mice contained IgA monomers and other nonpolymeric IgA forms. Thus, J chain is not essential for IgA transport by intestinal, mammary, or respiratory epithelia but is necessary for the stable association of pIgA with SC. Further, we suggest that J chain-deficient IgA is transported into secretions by a different mechanism than wild-type IgA.
authors: Division of Infectious Diseases, Children's Hospital, Boston, MA 02115, USA.
authors: B A
authors: BA
authors: Hendrickson
authors: L
authors: L
authors: Rindisbacher
authors: B
authors: B
authors: Corthesy
authors: D
authors: D
authors: Kendall
authors: D A
authors: DA
authors: Waltz
authors: M R
authors: MR
authors: Neutra
authors: J G
authors: JG
authors: Seidman
mesh_terms: D000818:Animals; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000235:genetics*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007144:Immunoglobulin J-Chains / Q000737:chemistry / Q000235:genetics*; D007413:Intestinal Mucosa / Q000276:immunology; D007774:Lactation; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D008892:Milk / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism; D012635:Secretory Component / Q000235:genetics*
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007144:Immunoglobulin J-Chains; D012635:Secretory Component