Neurogenesis of the sexually dimorphic vasopressin cells of the bed nucleus of the stria terminalis and amygdala of rats.
The bed nucleus of the stria terminalis (BNST) and centromedial amygdala share many neuroantomical and neurochemical characteristics, suggesting similarities in their development. Here we compare the neurogenesis of a group of cells for which already several common characteristics have been documented, that is, the sexually dimorphic arginine vasopressin-immunoreactive (AVP-ir) cells of the BNST and amygdala. To determine when these cells are born, pregnant rats received intraperitoneal injections of the thymidine analogue bromo-2-deoxy-5-uridine (BrdU) on one of nine embryonic days, E10 to E18; E1 being the day that a copulatory plug was found. At 3 months of age, the offsprings of these females were killed and their brains stained immunocytochemically for BrdU and AVP. Most AVP-ir cells were labeled with BrdU by injections on E12 and E13. Although BrdU labeling of AVP-ir cells did not differ between the BNST and amygdala, it differed between males and females. From E12 to E13, the percentage of BrdU-labeled AVP-ir cells decreased more in males than in females. AVP-ir cells appeared to be born earlier than most other cells in the same area, the majority of which were labeled with BrdU by injections on E14, E15, and E16. The similarities in the birthdates of AVP-ir cells in the BNST and amygdala may help to explain why these cells take on so many similar characteristics. The sex difference in birthdates of AVP-ir cells may help to explain which cellular processes underlie the sexual differentiation of these cells.
al-Shamma
H A
HA
Neuroscience and Behavior Program, University of Massachusetts, Amherst 01003-7710, USA.
De Vries
G J
GJ
D000679:Amygdala / Q000737:chemistry / Q000196:embryology*; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis*; D001973:Bromodeoxyuridine / Q000032:analysis; D005260:Female; D008297:Male; D051381:Rats; D017483:Receptors, Vasopressin / Q000737:chemistry; D012727:Sex Characteristics; D013788:Thalamus / Q000737:chemistry / Q000196:embryology*
D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D001973:Bromodeoxyuridine