Comparative analysis of somatostatin analog peptides by capillary electrophoresis and micellar elektrokinetic chromatography.
Capillary electrophoresis (CE) and micellar electrokinetic chromatography (MEKC) methods, utilizing uncoated silica capillary and triethyl ammonium phosphate or sodium borate buffers in the pH range of 2.25-11.0, containing sodium dodecyl sulfate (SDS) (0-100 mM) for analysis of somatostatin-analog peptides were developed. The method presented here was compared with the reversed-phase high performance liquid chromatographic (RP-HPLC) and CE methods developed for analysis of peptides. The peptides investigated in this work can be separated by CE on the basis of their electrophoretic mobility in aqueous buffer of low pH value (pH 2.25) or by MEKC on the basis of their hydrophobicity in SDS containing buffer of high pH value (pH 11.0). Optimal MEKC separation of the investigated peptides has been achieved at pH 11.0 in an Na-borate buffer containing 100 mM SDS. CE at pH 2.25 proved insensitive to the hydrophobicity of the peptides investigated. By contrast, results obtained with MEKC at pH 11.0 proved to be anologous to those obtained by RP-HPLC, with highly hydrophobic peptides-migrating slower than peptides without hydrophobic moieties.
Idei
M
M
First Department of Biochemistry, Semmelweis University Medical School, Budapest, Hungary. MIKI@PUSKIN.SOTE.HU
Mezö
I
I
Vadász
Z
Z
Horváth
A
A
Seprödi
J
J
Erchegyi
J
J
Teplán
I
I
Kéri
G
G
D002021:Buffers; D002845:Chromatography / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis; D013004:Somatostatin / Q000031:analogs & derivatives*; D013997:Time Factors
D002021:Buffers; D008823:Micelles; D010455:Peptides; D013004:Somatostatin