[Expression of hepatocyte growth factor and its receptor, C-Met in human digestive tissues and different gastric and colonic cancer cell lines].
OBJECTIVE
Hepatocyte growth factor (HGF) and its receptor c-Met exert mitogene and motogene activities in digestive tissues. The aim of the study was a) to detect and localize these proteins in adult human digestive mucosae, liver and pancreas, using western immunoblotting and immunohistochemistry with anti-HGF and c-Met antibodies and b) to identify the receptor in three digestive cancer cell lines.


RESULTS
HGF and c-Met were detected by the two techniques used in esophagus, fundus, antrum, duodenum, cecum, colon and rectum where they were localized in epithelial and sometimes lamina propria cells. HGF and c-Met were also present in hepatocytes, and c-Met in pancreatic endocrine cells. c-Met was identified in human gastric HGT-1 and rat colon DHD/K12 cancer cells. HGF (40 ng/mL) scattered colonies formed by these cells as well as human T84 colon cancer cells.


CONCLUSIONS
Our findings demonstrate the presence of HGF and c-Met in all human digestive tissues and are compatible with the implication of HGF in metastatic processes of digestive cancers.
Kermorgant
S
S
Unité INSERM 10, Hopital Bichat-Claude Bernard, Paris.
Cadiot
G
G
Lewin
M J
MJ
Lehy
T
T
D000328:Adult; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000737:chemistry*; D004064:Digestive System / Q000737:chemistry* / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000737:chemistry
D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases
Expression de I' "hepatocyte growth factor" et de son récepteur, c-Met, dans les tissus digestifs humains et certaines lignées cellulaires cancéreuses gastrique et coliques.