Haemophilus influenzae type b exoproducts induce chemotaxis and macrolide antibiotic release by human polymorphonuclear leukocytes.
The capacity of phagocytes to concentrate macrolide antibiotics was suggested by previous reports. In this study, we evaluated the capacity of Haemophilus influenzae type b culture supernatants (HICS) to induce polymorphonuclear leukocyte (PMNL) migration and macrolide antibiotic delivery. Using a Boyden multiwell chamber and a chemotaxis assay under agarose combined with a bioassay to measure antibiotic levels in agar, we demonstrated the chemotactic activity of HICS. Preincubation of PMNL with either erythromycin or azithromycin did not affect PMNL chemotaxis. By the agar diffusion test, we established that HICS increased the release of antibiotic from PMNL when compared with spontaneous release. Furthermore, we determined that the antibiotics remain bioactive after release. These results suggest that HICS may have a modulatory effect on transport and delivery of macrolide antibiotics by PMNL at the infection site.
Fontán
P A
PA
Departamento de Microbiología, Parasitología e Inmunología, Universidad de Buenos Aires, Argentina.
Buzzola
F R
FR
Spinedi
E G
EG
Sordelli
D O
DO
D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D017963:Azithromycin / Q000493:pharmacokinetics; D002634:Chemotaxis, Leukocyte; D004917:Erythromycin / Q000493:pharmacokinetics; D006193:Haemophilus influenzae / Q000502:physiology*; D006801:Humans; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology
D000900:Anti-Bacterial Agents; D004917:Erythromycin; D017963:Azithromycin