Oxatomide inhibits the release of proinflammatory mediators from human basophils and mast cells.
Oxatomide (OXA), a histamine H1 receptor antagonist, is effective in the treatment of patients with allergic rhinitis, some allergic skin disorders, and bronchial asthma. We have characterized the effect of OXA on the immunologic release of preformed (histamine and tryptase) and de novo synthesized mediators (leukotriene C4:LTC4 and prostaglandin D2:PGD2) from human basophils and mast cells purified (from 10 to 82%) from human lung parenchyma (HLMC) and skin tissue (HSMC). Preincubation (15 min, 37 degrees C) of basophils with OXA (10(-7)-10(-5) M) before Der p I antigen or anti-IgE challenge concentration-dependently (10-40%) inhibited the immunologic release of histamine and LTC4. OXA (10(-7)-10(-5) M) also inhibited (10-40%) histamine, tryptase and LTC4 release from HLMC activated by anti-IgE. In addition, OXA caused a concentration-dependent inhibition of histamine, tryptase and PGD2 release from HSMC immunologically challenged with a monoclonal antibody against the alpha chain of the high affinity receptor for IgE (anti-Fc epsilon RI) or anti-IgE. These results demonstrate that OXA exerts anti-inflammatory activities by inhibiting the release of preformed and de novo synthesized mediators from human basophils and mast cells.
Patella
V
V
Division of Clinical Immunology and Allergy, Faculty of Medicine, University of Naples Federico II, Italy.
de Crescenzo
G
G
Marino
O
O
Spadaro
G
G
Genovese
A
A
Marone
G
G
D000328:Adult; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D053818:Chymases; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D017997:Leukotriene C4 / Q000378:metabolism*; D008168:Lung / Q000737:chemistry / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D008875:Middle Aged; D010879:Piperazines / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D012867:Skin / Q000737:chemistry / Q000276:immunology; D053802:Tryptases
D006634:Histamine H1 Antagonists; D010879:Piperazines; D017997:Leukotriene C4; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases; C015408:oxatomide; D015230:Prostaglandin D2