High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris.
The early conceptus (embryo and associated membranes) of domestic ruminats signals its presence to the maternal uterus through production of interferon-tau (IFN-tau). Production of IFN-tau ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-tau (rOvIFN-tau) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-tau, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-tau at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-tau.
Van Heeke
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G
Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
Ott
T L
TL
Strauss
A
A
Ammaturo
D
D
Bazer
F W
FW
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D003470:Culture Media; D005285:Fermentation; D007370:Interferon Type I / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010843:Pichia; D010957:Plasmids / Q000235:genetics; D011257:Pregnancy Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012636:Secretory Rate; D012756:Sheep
D003470:Culture Media; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau