Localization of Epstein-Barr virus in lymph node metastasis with nasopharyngeal carcinoma.
Epstein-Barr virus DNA was detected in 3 cases of lymph node metastasis with nasopharyngeal carcinoma, using in situ hybridization. EBV-DNA was demonstrated on the nuclei of the neoplastic cells, and was absent on those of lymphocytes. Localization of EBV-DNA in neoplastic cells was confirmed by the double-labeling method combining in situ hybridization and immunocytochemistry. Direct evidence of the presence of EBV-DNA in malignant epithelial cells from lymph node metastasis with nasopharyngeal carcinoma was clearly demonstrated in this study.
Akao
I
I
Department of Otolaryngology, Ofuna Central Hospital, Kanagawa, Japan.
Sato
Y
Y
Mukai
K
K
Sugano
S
S
Hoshikawa
T
T
Suzuki
T
T
Tsutsumi
K
K
D002277:Carcinoma / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D046508:Culture Techniques; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000648:ultrastructure / Q000821:virology*; D009303:Nasopharyngeal Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D009305:Nasopharynx / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D009362:Neoplasm Metastasis