Characterization of a T cell line specific to an anti-Id antibody related to the carbohydrate antigen, sialyl SSEA-1, and the immundominant T cell antigenic site of the antibody.
The stage-specific embryonic Ag-1 (SSEA-1) is a carbohydrate Ag and regarded as an onco-developmental Ag. Sialyl SSEA-1 Ag, the sialylated form of SSEA-1, is frequently expressed in human cancer cells as well as in murine cancer cells. A mAb, FH-6, was shown to specifically recognize the Ag. We have generated five anti-Id Abs directed to the paratope-related idiotopes of the FH-6 Ab. One of these anti-Id Abs, Id-F2, increased the survival of host mice that were inoculated with Meth-A cells expressing the sialyl SSEA-1 Ag. To clarify the exact mechanism underlying the antitumor effect of the anti-Id Ab, we established a T cell line that recognized Id-F2 in association with MHC class II molecules. The T cell line was CD4+V beta 8+, and produced IL-2, exhibiting helper activity for B cells. The VH CDR2 region of the Id-F2 amino acid sequences turned out to be strongly immunogenic to T cells. When the immune complexes, consisting of the sialyl SSEA-1 Ag, FH-6, and Id-F2, were formed at the Meth-A cell-surface, the T cell line showed a strong proliferative response. The possible roles played by such T cell subsets in the anti-tumor effect are discussed.
Tsuyuoka
K
K
Laboratory of Experimental Pathology, Research Institute, Aichi Cancer Center, Nagoya, Japan.
Yago
K
K
Hirashima
K
K
Ando
S
S
Hanai
N
N
Saito
H
H
Yamasaki
K M
KM
Takahashi
K
K
Fukuda
Y
Y
Nakao
K
K
Kannagi
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000276:immunology; D002460:Cell Line; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology / Q000401:mortality; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007116:Immunization, Passive; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D016256:Lewis X Antigen / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D007135:Immunoglobulin Variable Region; D016256:Lewis X Antigen; D008748:Methylcholanthrene