Purification of subunit B of Shiga toxin using a synthetic trisaccharide-based affinity matrix.
The blood group P1 antigenic trisaccharide (3), which is the receptor-binding ligand of Shiga-like toxins, is synthesized in a spacer-equipped form (32) from 2-(trimethylsilyl)ethyl glucoside 5 and the 1-thiogalactoside building blocks 10 and 22 in a stereocontrolled, stepwise fashion. Covalent attachment of 32 to hydrazine group-containing agarose gel by reductive amination provided the P1 trisaccharide-containing affinity sorbent which was used for preparative scale isolation of subunit B of Shiga toxin.
Pozsgay
V
V
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Trinh
L
L
Shiloach
J
J
Robbins
J B
JB
Donohue-Rolfe
A
A
Calderwood
S B
SB
D001331:Automation; D001427:Bacterial Toxins / Q000737:chemistry / Q000302:isolation & purification*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D004768:Enterotoxins / Q000737:chemistry / Q000302:isolation & purification; D005782:Gels; D006027:Glycosides; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010127:P Blood-Group System; D012685:Sepharose; D022603:Shiga Toxins; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D014734:Vibrio cholerae
D001427:Bacterial Toxins; D004768:Enterotoxins; D005782:Gels; D006027:Glycosides; D007202:Indicators and Reagents; D010127:P Blood-Group System; D022603:Shiga Toxins; D014312:Trisaccharides; D012685:Sepharose