Active expression of G gamma globin gene on chromosome 11 with Yunnanese A gamma delta beta)0-thalassemia deletion in MEL cells.
A permanent lymphocyte cell line of a heterozygote with Yunnanese (A gamma delta beta)0-thalassemia deletion, associated with an increased production of G gamma globin in adult, was founded using Epstein-Barr virus transformation. The hybrids of the lymphocyte cell and mouse erythroleukemia cell (MEL) were achieved and the hybrids containing human chromosome 11 were selected with the monoclonal antibody 53/6. The subclones containing only either the normal or the abnormal human chromosome 11 were separated and the expression of the human globin genes was studied. Expression of the beta-globin gene, but not the G gamma and A gamma, was observed in the hybrids containing only the normal human chromosome 11, while active expression of the G gamma globin gene was observed in the hybrids containing only the abnormal human chromosome 11. These results have confirmed that the DNA deletion in the beta-globin gene cluster is the cause of persistent active expression of the G gamma globin gene in the Yunnanese mutant.
Zhang
J
J
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Qiao
J
J
Song
W
W
Qiu
Z
Z
D000328:Adult; D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D017353:Gene Deletion; D015870:Gene Expression; D005914:Globins / Q000235:genetics*; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology; D008297:Male; D051379:Mice; D013789:Thalassemia / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D005914:Globins