An enzyme-linked immunosorbent assay (ELISA) for the detection of antibody against Streptococcus suis type 2 in infected pigs.
An ELISA test for the detection of antibody against S. suis type 2 in pigs was developed and applied to field sera. The best sensitivity and specificity of ELISA were obtained when a purified polysaccharide antigen was used. It showed no cross reaction with sera against other serotypes of S. suis and other pathogenic bacteria. A total of 264 sera were collected from 20 pig farms and examined with the antibody against S. suis type 2. In the affected farms, 17.0% of pigs tested were positive, 9.8% in the adjacent farms, but only 3.4% in the free farms. The difference of the positive percentages between the affected and the free farms was statistically significant (P < 0.05).
Kataoka
Y
Y
Department of Veterinary Microbiology, Nippon Veterinary and Animal Science University, Musashino, Japan.
Yamashita
T
T
Sunaga
S
S
Imada
Y
Y
Ishikawa
H
H
Kishima
M
M
Nakazawa
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011237:Predictive Value of Tests; D012016:Reference Values; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D017011:Streptococcus suis / Q000276:immunology; D013552:Swine; D013553:Swine Diseases
D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial