title: Ultrastructure of hepatocytes in copper-deficient Sika deer (Cervus nippon Temminck).
abstract: The livers of 13 Sika deer (Cervus nippon Temminck) aged 4 to 9 years and suffering from copper deficiency (enzootic ataxia) were examined histologically, histochemically and by electron microscopy. In addition, the serum and liver copper concentrations, measured in three animals, were found to be low. Histologically, the hepatocytes exhibited cloudy swelling, and numerous haemosiderin deposits were seen in the hepatocytes and Kupffer cells. Staining with p-dimethyl amino-benzylidene-rhodamine revealed distinctly fewer copper granules than normal. Histochemically, 3,3'-diaminobenzidine-H2O2 staining revealed increased numbers of catalase-positive granules around nuclei. Electron microscopically, "giant" and bizarre-shaped mitochondria, irregular depression of the mitochondrial membrane, and fusion of cristae were noted. Disorders of copper-containing enzymes, including cytochrome oxidase, caeruloplasmin and monoamine oxidase, may have been responsible for the mitochondrial abnormalities.
authors: Department of Veterinary Pathology, School of Medicine and Animal Sciences, Kitasato University, Japan.
authors: H
authors: H
authors: Seo
authors: B
authors: B
authors: Xie
authors: S
authors: S
authors: Wang
authors: H
authors: H
authors: Yoshikawa
authors: T
authors: T
authors: Oyamada
authors: T
authors: T
authors: Yoshikawa
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D002570:Ceruloplasmin / Q000032:analysis; D003300:Copper / Q000032:analysis / Q000097:blood / Q000172:deficiency*; D003670:Deer / Q000033:anatomy & histology*; D004196:Disease Outbreaks / Q000662:veterinary; D003576:Electron Transport Complex IV / Q000032:analysis; D005260:Female; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D008930:Mitochondria, Liver / Q000648:ultrastructure; D008995:Monoamine Oxidase / Q000032:analysis; D012756:Sheep; D013540:Swayback / Q000453:epidemiology / Q000209:etiology / Q000473:pathology*
chemical_list: D003300:Copper; D002570:Ceruloplasmin; D008995:Monoamine Oxidase; D003576:Electron Transport Complex IV