Polyamine amides are neuroprotective in cerebellar granule cell cultures challenged with excitatory amino acids.
Primary cultures of rat cerebellar granule cells have been used to assess the potential neuroprotective effects of philanthotoxins and argiotoxin-636 (ArgTX-636). These polyamine amides are potent antagonists of ionotropic L-glutamate (L-Glu) receptors. In granule cells loaded with fluo-3, ArgTX-636 and philanthotoxin-343 (PhTX-343) antagonised increases of intracellular free calcium concentration ([Ca2+]i) that were stimulated by N-methyl-D-aspartate (NMDA). The antagonism was use-dependent. Antagonism by PhTX-343 was fully reversible, but recovery following antagonism by ArgTX-636 was slow and only partial during the time-course of an experiment. Neither compound inhibited K(+)-induced increases in [Ca2+]i. In excitotoxicity studies with cerebellar granule cells, the release of lactate dehydrogenase (LDH) and morphological observations were used to assess cell death. A 20-30 min exposure to 500 microM NMDA, 100 microM L-Glu or 500 microM kainate was sufficient to kill > 90% of the cells after 18-20 h. When added 5 min prior to, and during agonist exposure, PhTX-343 and ArgTX-636 provided total neuroprotection. ArgTX-636 was about 20-30 fold more potent than PhTX-343 against NMDA, but was approximately equipotent with PhTX-343 against a kainate challenge. Neither of the toxins showed any inherent toxicity even at 400 microM and 100 microM respectively. Some analogues of PhTX-343 are more potent, both in terms of antagonism of NMDA-stimulated increases of [Ca2+]i and neuroprotection, than PhTX-343 and ArgTX-636.
Green
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AC
Department of Life Science, University of Nottingham, University Park, UK.
Nakanishi
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K
Usherwood
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PN
D000577:Amides; D000818:Animals; D001180:Arthropod Venoms / Q000633:toxicity; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology; D007210:Indoleacetic Acids; D007608:Kainic Acid / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010636:Phenols / Q000633:toxicity*; D010648:Phenylacetates / Q000494:pharmacology*; D011073:Polyamines / Q000494:pharmacology* / Q000633:toxicity*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013111:Spider Venoms / Q000494:pharmacology
D000577:Amides; D001180:Arthropod Venoms; D018690:Excitatory Amino Acid Agonists; D007210:Indoleacetic Acids; D018696:Neuroprotective Agents; D010636:Phenols; D010648:Phenylacetates; D011073:Polyamines; D013111:Spider Venoms; C558368:argiotoxin-636; C074003:philanthotoxin 343; D018698:Glutamic Acid; D016202:N-Methylaspartate; D011188:Potassium; D007608:Kainic Acid; D002118:Calcium