Recovery efficiency of Cryptosporidium from water with a crossflow system and continuous flow centrifugation: a comparison study.
A crossflow system and continuous flow centrifugation were tested with regard to the recovery rate of Cryptosporidium oocysts from water. Equal volumes of tap water were contaminated with specific numbers of oocysts. With the crossflow system, an average recovery of 9.8% was obtained when using a concentration of 2 x 10(2) oocysts/L. Recovery rates achieved with the continuous flow centrifuge were more satisfactory: Starting with a concentration of 80 oocysts/L, we obtained an average recovery of 12.9%. The technique of continuous flow centrifugation was found to be more efficient for collection of Cryptosporidium oocysts from water than the crossflow system.
Renoth
S
S
Institut für Medizinische Parasitologie, Universität Bonn, Germany.
Karanis
P
P
Schoenen
D
D
Seitz
H M
HM
D000818:Animals; D002498:Centrifugation; D003458:Cryptosporidium / Q000302:isolation & purification*; D005374:Filtration; D014867:Water / Q000469:parasitology*
D014867:Water