Immunolocalizations of human gelatinase (type IV collagenase, MMP-9) and TIMP (tissue inhibitor of metalloproteinases) in normal epidermis and some epidermal tumors.
The matrix metalloproteinases (MMPs) MMP-2 and MMP-9 (gelatinases) have been suggested as serving an important role in cleaving the basement membrane structure. Tissue inhibitors of metalloproteinases TIMPs (particularly TIMP-1) are known to inhibit MMPs. Based on this background, we raised monoclonal antibodies against human gelatinase (MMP-9) and human recombinant TIMP (TIMP-1), and immunostained these two components in skin from patients with squamous cell carcinoma (SCC), Bowen's disease (BD) and keratoacanthoma (KA). MMP-9 showed positive staining mainly in the granular layer of normal epidermis. In some cases of SCC and BD, MMP-9 showed positive staining in the dysplastic lesions even in the basal layer. TIMP showed a thorough positivity in normal epidermis. Unstained regions with this antibody were observed in SCC and BD. These results suggest that an altered staining pattern for MMP-9 and TIMP may be closely related to the malignant transformation of SCC and BD.
Kobayashi
T
T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Onoda
N
N
Takagi
T
T
Hori
H
H
Hattori
S
S
Nagai
Y
Y
Tajima
S
S
Nishikawa
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D001913:Bowen's Disease / Q000201:enzymology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D017364:Collagenases / Q000378:metabolism*; D004817:Epidermis / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007636:Keratoacanthoma / Q000201:enzymology; D008297:Male; D020780:Matrix Metalloproteinase 9; D051379:Mice; D008807:Mice, Inbred BALB C; D011480:Protease Inhibitors / Q000378:metabolism; D012016:Reference Values; D012878:Skin Neoplasms / Q000208:ethnology*; D014018:Tissue Distribution; D019714:Tissue Inhibitor of Metalloproteinases
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases; D020780:Matrix Metalloproteinase 9