Characterization of the natural immune response of rhesus monkey CD4+ve T cells to the bacterial antigen streptolysin O (SLO).
Rhesus monkeys show a high proliferative T cell response to the bacterial exotoxin SLO without prior immunization. The present study was undertaken to characterize this naturally present SLO-responsiveness with particular emphasis on CD4+ve reactive T cells. It is demonstrated that the frequency of SLO-reactive cells in the circulation.ranges between 1 in 75 and 1 in 610 CD4+ve T cells as determined with limiting dilution analysis. It is also shown that induction of a good proliferative response requires Mhc-DR matching between T cell and the antigen presenting cells (APC). Stable and DR-restricted SLO-specific CD4+ve T cell lines were generated from CD8 depleted peripheral blood mononuclear cells (PBMC). The SLO-reactive CD4+ve cell lines are tentatively characterized as Th1-like based on the predominant production of interferon-gamma (IFN-gamma) over IL-4, although this seems contradicted by the IL-4 dependent growth of the lines.
Kraakman
E M
EM
Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Bontrop
R E
RE
Groenestein
R
R
Jonker
M
M
Haaijman
J J
JJ
t Hart
B A
BA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007113:Immunity, Innate; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008253:Macaca mulatta; D012044:Regression Analysis; D013301:Streptolysins / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
D001426:Bacterial Proteins; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D013301:Streptolysins; C025964:streptolysin O; D015847:Interleukin-4; D007371:Interferon-gamma