Preclinical evaluation of single-cell oils that are highly enriched with arachidonic acid and docosahexaenoic acid.
Arachidonic acid (ARA) and docosahexaenoic acid (DHA) are important in human brain and retina development, and there is growing evidence showing the importance of these fatty acids in infant nutrition. Triglyceride oils, highly enriched in ARA (ARASCO) and DHA (DHASCO), were evaluated using very high dose acute (20 g/kg) and 4-wk subchronic gavage feedings in weanling Sprague-Dawley rats. The combination of these oils, Formulaid, was also tested in the 4-wk subchronic study, ARASCO, DHASCO and Formulaid were found to have a no-observable-adverse-effect level of more than 2.5 g/ kg/day, 1.25 g/kg/day and 3.75 g/kg/day, respectively. This represents a 50-fold safety margin over the intended use of Formulaid in infant formula. Survival, clinical signs, body weight gain, food consumption, haematology, clinical chemistry and histopathological evaluations failed to show any significant differences in animals administered ARASCO, DHASCO or Formulaid compared with that in control animals administered equal amounts of high oleic sunflower oil. The bioavailability of ARASCO, DHASCO and Formulaid was verified by increases in DHA and ARA levels in heart and liver tissues in these animals. Because these oils are enriched in only a single bioactive fatty acid, and they have been shown to be safe, they may offer a new source of these fatty acids in speciality foods such as infant formula.
Boswell
K
K
Martek Biosciences Corporation, Columbia, MD 21045, USA.
Koskelo
E K
EK
Carl
L
L
Glaza
S
S
Hensen
D J
DJ
Williams
K D
KD
Kyle
D J
DJ
D000284:Administration, Oral; D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D001923:Brain Chemistry; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000633:toxicity*; D004435:Eating / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D007928:Lethal Dose 50; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000737:chemistry; D009929:Organ Size / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000737:chemistry
D005227:Fatty Acids; D010938:Plant Oils; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid