A method for rapid determination of zotepine by gas chromatography-mass spectrometry.
A rapid and sensitive method using solid-phase extraction and gas chromatography-mass spectrometry (GC-MS) has been developed for the determination of zotepine (ZTP), an atypical neuroleptic, in human plasma. The detection limit of ZTP was 1 microgram/L. Standard curves over the concentration range from 2.5 to 100 micrograms/L had a good linearity. Intraassay variability ranged from 2.2 to 3.3% and interassay variability from 3.5 to 6.6% at the concentration range of 5-75 micrograms/L. Our preliminary data of single-dose kinetics of ZTP by using this method suggested that the peak time and elimination half-life was much longer than previously reported, and that there appeared to be a second peak after 10-12 h of ZTP administration, indicating the possibility of the presence of enterohepatic recirculation.
Tanaka
O
O
Department of Neuropsychiatry, Hirosaki University School of Medicine, Japan.
Kondo
T
T
Kaneko
S
S
Mihara
K
K
Otani
K
K
Tokinaga
N
N
D014150:Antipsychotic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D003987:Dibenzothiazepines / Q000097:blood; D003988:Dibenzothiepins / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans
D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D003988:Dibenzothiepins; C022172:zotepine; C084602:clothiapine