A natural kinase-deficient variant of fibroblast growth factor receptor 1.
A fibroblast growth factor receptor 1 variant missing 37 amino acids from the carboxy-terminal tyrosine kinase catalytic domain was discovered in human lung fibroblasts and several other human cell lines. The receptor variant binds specifically to acidic fibroblast growth factor but has no tyrosine kinase activity. It was found that cellular transfectants expressing the fibroblast growth factor receptor 1 variant are mitogenically inactive and ligand binding to the receptor causes neither receptor autophosphorylation nor phospholipase C-gamma transphosphorylation. The fibroblast growth factor receptor 1 variant therefore represents an inactive receptor for acidic fibroblast growth factor. Since both kinase and kinase-deficient receptor forms are expressed in cells, it is conceivable that the kinase-deficient receptor plays an important role in regulating cellular responses elicited by acidic fibroblast growth factor stimulation.
Wang
L Y
LY
Howard Hughes Medical Institute, Department of Medicine, University of California San Francisco, U-426, 94143-0724, USA.
Edenson
S P
SP
Yu
Y L
YL
Senderowicz
L
L
Turck
C W
CW
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts; D014644:Genetic Variation; D006801:Humans; D008168:Lung / Q000201:enzymology; D008969:Molecular Sequence Data; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D015347:RNA Probes; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases; D017384:Sequence Deletion; D013936:Thymidine / Q000378:metabolism; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D015347:RNA Probes; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1; C496348:FGFR1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D012260:Ribonucleases; D013936:Thymidine