Second transplantation with CD34+ bone marrow cells selected from a two-loci HLA-mismatched sibling for a patient with chronic myeloid leukaemia.
A 43-year-old man with chronic myeloid leukaemia underwent a second transplant with CD34+ bone marrow cells selected from his two-loci HLA-mismatched sibling after rejection of the first graft from an HLA-matched unrelated donor. By immunomagnetic positive selection, CD34+ marrow cells at 0.95 x 10(6)/kg with 97% purity and CD3+ T lymphocytes at 1.3 x 10(4)/kg were collected and transplanted. Engraftment was confirmed to be of CD34+ cell-donor origin. The patient developed only grade I acute graft-versus-host disease (GVHD) and no chronic GVHD to date. These observations suggest that allogeneic CD34+ bone marrow cells are capable of reconstituting haemopoiesis and that CD34+ selection could be applicable to T-cell depletion.
Fujimori
Y
Y
Second Department of Internal Medicine, Hyogo College of Medicine, Japan.
Kanamaru
A
A
Hashimoto
N
N
Okamoto
T
T
Okada
M
M
Kawaguchi
K
K
Mori
A
A
Saheki
K
K
Takatsuka
H
H
Wada
H
H
Takemoto
Y
Y
Kohsaki
M
M
Imai
N
N
Kakishita
E
E
Nagai
K
K
D000328:Adult; D018952:Antigens, CD34; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000379:methods*; D006084:Graft Rejection; D006086:Graft vs Host Disease / Q000209:etiology / Q000517:prevention & control; D006650:Histocompatibility Testing; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy*; D008231:Lymphopenia / Q000209:etiology; D008297:Male; D013601:T-Lymphocytes
D018952:Antigens, CD34; D000961:Antilymphocyte Serum