In vitro senescence enhances IL-6 production in human gingival fibroblasts induced by lipopolysaccharide from Campylobacter rectus.
The production of interleukin-6 (IL-6) in human gingival fibroblasts (Gin cells) is increased by lipopolysaccharide (LPS) from Campylobacter rectus (C. rectus), which is associated with adult periodontitis; however, the age-related changes in the susceptibility of Gin cells to C. rectus LPS remain unclear. We examined the influence of in vitro senescence on C. rectus LPS-stimulated IL-6 production in Gin cells. LPS was prepared from C. rectus ATCC 33238 using hot phenol-water. The Gin cells were established from healthy gingival tissue removed from three patients, aged 10-12 years. The cells were cultured until confluence then stimulated with LPS (0.01, 0.1, 1.0 and 10.0 micrograms/ml). Levels of IL-6 released in the medium were measured after incubation for 3, 6, 9, 12, and 24 h. In both young (5-6 population doublings) and senescent (17-20 population doublings) cells, LPS stimulated IL-6 production in a dose- and time-dependent manner. In response to 0.01-10.0 micrograms/ml of LPS, IL-6 production in the senescent cells was higher than that in the young cells. Using cells from each of the three donors, we found that this phenomenon of higher LPS-stimulated IL-6 production in senescent cells was reproducible. The greater capacity of the senescent cells to synthesize IL-6 in response to LPS was a higher production of mRNA for IL-6. This increase of IL-6 production induced by C. rectus LPS in senescent Gin cells could help to explain the increased susceptibility to periodontal diseases shown by aged individuals.
Ogura
N
N
Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Matsuda
U
U
Tanaka
F
F
Shibata
Y
Y
Takiguchi
H
H
Abiko
Y
Y
D000328:Adult; D001483:Base Sequence; D002167:Campylobacter / Q000472:pathogenicity; D016922:Cellular Senescence / Q000276:immunology*; D002648:Child; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D015870:Gene Expression; D005881:Gingiva / Q000166:cytology / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D010518:Periodontitis / Q000209:etiology; D012333:RNA, Messenger / Q000235:genetics
D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger