Type II muscle fibers are stained by anti-Fas antibody.
To examine whether apoptosis related proteins are present in skeletal muscles we studied biopsied muscles immunohistochemically and by Western blot analysis. Biopsied muscles from patients with several disorders were studied with anti-Fas antibody and anti-BCL2 antibody. Type II muscle fibers identified by ATPase staining were positively stained by anti-Fas antibody in both normal control and diseased muscles. Anti-BCL2 antibody did not stain any muscle fibers. Western blot analysis using anti-Fas antibody showed a single band at 45 kDa in both skeletal muscle and lymphocytes. Anti-Fas antibody has been reported to induce apoptosis in the cells. The presence of anti-Fas antibody reactive materials in type II muscle fibers might be related to type II fiber atrophy in muscular disorders.
Yamada
H
H
Third Department of Internal Medicine, Kagoshima University School of Medicine, Japan.
Nakagawa
M
M
Higuchi
I
I
Ohkubo
R
R
Osame
M
M
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D006801:Humans; D007150:Immunohistochemistry; D018485:Muscle Fibers, Skeletal / Q000737:chemistry*; D009124:Muscle Proteins / Q000032:analysis*; D019014:fas Receptor / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D009124:Muscle Proteins; D019014:fas Receptor