Isolation and characterization of the restriction endonuclease PpeI from Phormidium persicinum.
PpeI is a type II restriction endonuclease isolated from cyanobacterial strain Phormidium persicinum. The endonuclease PpeI, an isoschizomer of ApaI, recognizes the hexanucleotide sequence (5'-GGGCC/C-3') and cleaves, after the second C, producing four nucleotide 3'-cohesive ends.
Piechula
S
S
Department of Microbiology, University of Gdańsk, Poland.
Piosik
J
J
Bielawski
K
K
Podhajska
A J
AJ
D010582:Bacteriophage lambda / Q000235:genetics; D000458:Cyanobacteria / Q000201:enzymology*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013696:Temperature
D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases