Modification of inositol 1,4,5-trisphosphate concentration of human erythrocytes under "in vivo" physiological conditions.
The concentration of inositol 1,4,5-trisphosphate (InsP3) in erythrocytes from volunteers has been found to modify following strenuous physical exercise. The basal value was almost regained within some 75 min after the completion of the effort. The concurrent variations of pH and blood lactate have also been evaluated. Our results represent, to our knowledge, the first evidence of in vivo induced intraerythrocyte InsP3 modification. They reinforce the idea of the participation of its precursor phosphatidylinositol 4,5-bisphosphate (PtdIns4,5-P2) in red bood cell shape regulation by contributing to the interactions between membrane and cytoskeleton components.
Piacentini
M P
MP
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Italy.
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D000293:Adolescent; D000328:Adult; D004912:Erythrocytes / Q000378:metabolism* / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000097:blood*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000097:blood
D007773:Lactates; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D019344:Lactic Acid; D015544:Inositol 1,4,5-Trisphosphate