Thirteen loci physically assigned to sheep chromosome 2 by cell hybrid analysis and in situ hybridization.
Sheep x hamster cell hybrids containing sheep metacentric Chromosome (Chr) 2 were produced by fusing blood leukocytes from normal sheep with hamster auxotrophic Ade F-minus mutants. Cell clones that were isocitrate dehydrogenase 1 (IDH1) positive were cytogenetically characterized, confirming that they contained sheep Chr 2. The following loci were newly assigned by Southern hybridization to sheep Chr 2: lipoprotein lipase (LPL), glycoprotein-4-beta galactosyltransferase 2 (GGTB2), neurofilament light polypeptide (68 kDa; NEFL), surfactant-associated protein 2 (SFTP2), lymphocyte-specific protein tyrosine kinase (LCK), and nebulin (NEB). These new assignments and the in situ localization of gelsolin (GSN) to sheep Chr 2pter-p24 are consistent with the predicted homology of cattle Chr 8 (U18) with sheep Chr 2p, and of cattle Chr 2 (U17) with sheep 2q. In addition, the assignment by cell hybrid analysis of loci previously mapped to Chr 2 in sheep, viz., cholinergic receptor, nicotinic, delta polypeptide (CHRND), collagen type III alpha 1 (COL3A1), fibronectin 1 (FN1), isocitrate dehydrogenase (IDH1), and villin 1 (VIL1), confirmed the localization of sheep syntenic group U11 to this chromosome. By nutritional selection and complementation of the hamster auxotrophic Ade F mutation, the multifunctional enzyme locus phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase)/IMP cyclohydrolase (inosinicase) (provisionally given the symbol PRACFT) has also been newly assigned to sheep Chr 2. This report significantly extends the number of loci physically mapped to sheep Chr 2 and confirms its close homology with cattle Chrs 2 and 8.
Broad
T E
TE
AgResearch Grasslands Research Centre, Palmerston North, New Zealand.
Lewis
P E
PE
Burkin
D J
DJ
Gleeson
A J
AJ
Carpenter
M A
MA
Jones
C
C
Pearce
P D
PD
Maher
D W
DW
Ansari
H A
HA
D000818:Animals; D015139:Blotting, Southern; D016466:CHO Cells; D002874:Chromosome Mapping; D006224:Cricetinae; D018260:Gelsolin / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D012756:Sheep / Q000235:genetics*
D018260:Gelsolin