Simple analysis of plasmalogens in erythrocytes using gas chromatography/mass spectrometry with selected-ion monitoring acquisition.
Plasmalogens are a unique class of ether-phospholipids whose role, even though not fully defined, is essential. Their biosynthesis starts in peroxisomes, therefore, plasmalogen analysis is fundamental in the study of peroxisomal disorders. The present work reports a simple method for plasmalogen determination in erythrocytes for use in the study of peroxisomal disorders in humans; the procedure is based on two other methods that have previously been reported and employs GC/MS for separation and detection.
Caruso
U
U
University Department of Pediatrics, G. Gaslini Institute, Genova, Italy.
D000080:Acetals / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry*; D005227:Fatty Acids / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010955:Plasmalogens / Q000097:blood*
D000080:Acetals; D005227:Fatty Acids; D010955:Plasmalogens