Interferon gamma production in whole peripheral blood culture in acute hepatitis B.
Interferon gamma (IFNg) production in whole peripheral blood (WPB) and mononuclear (MN) cell culture in acute hepatitis B (AHB) was compared. IFNg production was induced by phytogem agglutinin and measured in the cell supernatants of 14 AHB patients in the course of the disease. There were some up-regulating factors of IFNg production that probably operated in WPB culture: the presence of autoerythrocytes as well as the low content of monocytes. Autoserum regulated IFNg production in a stage-dependent way: it decreased IFNg activity at the bilirubin peak in hepatitis B infection, but not in convalescence. In contrast, we did not find a serum blocking effect in the corresponding stage of acute hepatitis A. The nature of this serum blocking factor in AHB is unclear.
Osna
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N
Laboratory of Immunology and Immunogenetics, Latvian Medical Academy, Riga.
Duk
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A
Sochnev
A
A
D000208:Acute Disease; D001663:Bilirubin / Q000097:blood; D001773:Blood Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D006509:Hepatitis B / Q000097:blood* / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins; D007371:Interferon-gamma; D001663:Bilirubin