Determination of melengestrol acetate in supercritical fluid-solid phase extracts of bovine fat tissue by HPLC-UV and GC-MS.
A method is developed for the determination of melengestrol acetate in bovine fat tissue at or less than the established tolerance level of 25 ppb. The procedure uses a combination of supercritical fluid extraction (SFE) and solid-phase extraction (SPE) techniques to produce an extract suitable for analysis with either high-performance liquid chromatography with ultraviolet detection or gas chromatography-mass spectrometry. Overall recovery of the analyte from bovine fat tissue is 99.4% with a coefficient of variation of 4.14%. The SFE-SPE procedure uses a total of 12 mL of organic solvent per fat tissue sample versus more than 1.7 L consumed in current extraction procedures.
Parks
O W
OW
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA.
Shadwell
R J
RJ
Lightfield
A R
AR
Maxwell
R J
RJ
D000273:Adipose Tissue / Q000737:chemistry*; D000738:Androsterone / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008552:Melengestrol Acetate / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014020:Tissue Extracts / Q000032:analysis
D014020:Tissue Extracts; D008552:Melengestrol Acetate; D000738:Androsterone