Cytokine production by human epithelial and endothelial cells following exposure to oral viridans streptococci involves lectin interactions between bacteria and cell surface receptors.
In order to examine the possible implication of human epithelial and endothelial cells in the pathogenesis of various diseases associated with oral viridans streptococci, we tested the immunomodulatory effects of 11 representative strains of oral viridans streptococci on human epithelial KB cells and endothelial cells. We then examined the possible role of two major adhesins from oral viridans streptococci, protein I/II and rhamnose-glucose polymers (RGPs), in this process. In this study we demonstrate that oral viridans streptococci are potent stimulators of interleukin-8 (IL-8) production from KB cells and of IL-6 and IL-8 production from endothelial cells. The ability of protein I/II and RGPs to contribute to these effects was then examined. Using biotinylated protein I/IIf and RGPs from Streptococcus mutans OMZ 175, we showed that these adhesins bind to KB and endothelial cells through specific interactions and that the binding of these molecules initiates the release of IL-8 from KB cells and of IL-6 and IL-8 from endothelial cells. These results suggest that protein I/IIf and RGPs play an important role in the interactions between bacteria and KB and endothelial cells in that similar cytokine profiles are obtained when cells are stimulated with bacteria or surface components. We also provide evidence that protein I/IIf binds to and stimulates KB and endothelial cells through lectin interactions and that N-acetyl neuraminic acid (NANA) and fucose present on cell surface glycoproteins may form the recognition site since binding and cytokine release can be inhibited by dispase and periodate treatment of cells and by NANA and fucose. These results demonstrate that oral viridans streptococci, probably by engaging two cell surface adhesins, exert immunomodulatory effects on human KB and endothelial cells.
Vernier
A
A
Faculté de Pharmacie, Institut National de la Santé et de la Recherche Médicale Unité 392, Illkirch, France.
Diab
M
M
Soell
M
M
Haan-Archipoff
G
G
Beretz
A
A
Wachsmann
D
D
Klein
J P
JP
D018829:Adhesins, Bacterial / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D037102:Lectins / Q000378:metabolism*; D008562:Membrane Glycoproteins; D009055:Mouth / Q000382:microbiology; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011975:Receptors, Mitogen / Q000378:metabolism; D013291:Streptococcus / Q000472:pathogenicity*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D037102:Lectins; D008562:Membrane Glycoproteins; D011135:Polysaccharides, Bacterial; D011975:Receptors, Mitogen; C055764:S-layer proteins