Immunoregulatory effects of two bone-marrow hexapeptides (myelopeptides) in experimental models of immunodeficiency.
Myelopeptide 1 (MP-1, Phe-Leu-Gly-Phe-Pro-Thr) and myelopeptide 2 (MP-2, Leu-Val-Val-Tyr-Pro-Trp) are hexapeptides originally isolated from porcine bone-marrow cell culture. MP-1 enhanced the number of antibody-forming cells in NZB mouse spleen cells in vitro and increased the antibody production in Cy-treated (CBAxC57B1/6)F1 mice in vivo. MP-1 increased ConA-induced proliferation of both NZB spleen cells in vitro and spleen cells of Cy-treated mice in vivo. MP-1 restored polyclonal IgG synthesis depressed by PWM in the NZB spleen cell culture. MP-2 also evoked immunoregulatory effects which were less pronounced and realized at higher concentrations than effects of MP-1. Neither MP-1 nor MP-2 affected LPS-induced spleen cell proliferation in both NZB- and Cy-treated (CBAxC57B1/6)F1 mice.
Mikhailova
A A
AA
Department of Immunology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Shanurin
S Y
SY
Petrov
R V
RV
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003520:Cyclophosphamide; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000139:chemically induced / Q000276:immunology*; D007166:Immunosuppressive Agents; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008814:Mice, Inbred NZB; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010455:Peptides / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D009842:Oligopeptides; D010455:Peptides; C048617:myelopeptides; D003520:Cyclophosphamide