[Effect of neuromedin C on gastrin secretion from isolated and perfused rat stomach].
Using a model of isolated and perfused rat stomach, it was observed that the gastrin secretion was increased significantly by intravascular perfusion of 10(-8) mol/l neuromedin C (NMC) (from 550 +/- 126 pg/10 min to 1060 +/- 180 pg/10 min, P < 0.01). The effect was in part suppressed by atropine (10(-7) mol/L) (756 +/- 152 pg/10 min v.s. 1060 +/- 180 pg/10 min, P < 0.05) and completely blocked by D-Phe6-BN (6-13) OMe (10(-6) mol/L), a bombesin receptor antagonist from human antral G-cells. These results indicate that NMC is a powerful stimulant for gastrin secretion mostly by directly stimulating G-cells and, to some extent, by stimulating cholinergic pathway. It was also suggested that the bombesin-related receptor in rat stomach is similar to that in the human G-cells.
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Department of Pathophysiology, Zunyi Medical College.
D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D005755:Gastrins; D010446:Peptide Fragments; C071101:bombesin (6-13), Phe(6) methyl ester-; C040794:neuromedin C; D001839:Bombesin