Production of lytic enzyme from Pseudomonas aeruginosa M-1001.
Pseudomonas aeruginosa M-1001 produced both hen-egg-white lysozyme inhibitors and lytic enzyme in the culture broth. The lytic enzyme produced was not inhibited by the lysozyme inhibitors. Maximal lytic activity was obtained when the strain was grown aerobically in a medium consisting of 0.25% glucose, 0.75% soluble strach, 0.25% beef extract, 0.25% Polypepton, 0.25% sodium L-glutamate and 0.0001% NaCl (pH 6), at 37 degrees C after 36 hrs. The lytic enzyme was stable at pH from 6 to 8 and temperatures below 40 degrees C. Many bacteria and Saccharomyces cerevisiae were found to be inhibited by the crude lytic enzyme produced from the M-1001 strain except for the strain itself and a few other microorganisms.
Wang
S L
SL
Department of Food Engineering, Da-Yeh Institute of Technology, Chang-Hwa, Taiwan, Republic of China.
Pai
C S
CS
Shieh
S T
ST
D000332:Aerobiosis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D002645:Chickens; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013379:Substrate Specificity
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004791:Enzyme Inhibitors; D009113:Muramidase