Tubulogenesis from isolated single cells of adult mammalian kidney: clonal analysis with a recombinant retrovirus.
The adult mammalian kidney tubule epithelium exists in a relatively dormant, slowly replicative state but has a large potential for regenerative morphogenesis following severe ischemic or toxic injury. Under selective serum-free growth conditions, which included epidermal growth factor and retinoic acid, a subpopulation of renal proximal tubule cells isolated from adult rabbit kidney were grown in cell culture. These cells possessed two important characteristics: 1) an ability to differentiate morphogenically into tubule structures when grown in three-dimensional collagen gels and 2) a high capacity for self-renewal, since cell lineage analysis with a recombinant retrovirus demonstrated that in vitro tubulogenesis arose from clonal expansion of a single cell. Thus individual cells in the adult kidney have retained the ability for kidney tubulogenesis in vitro.
Humes
H D
HD
Department of Internal Medicine, Veterans Affairs Medical Center, Ann Arbor, Michigan, USA.
Krauss
J C
JC
Cieslinski
D A
DA
Funke
A J
AJ
D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells; D003094:Collagen; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005782:Gels; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D011817:Rabbits; D011995:Recombination, Genetic; D012038:Regeneration; D012190:Retroviridae / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology; D014212:Tretinoin / Q000494:pharmacology
D005782:Gels; D014212:Tretinoin; D004815:Epidermal Growth Factor; D003094:Collagen