High-performance liquid chromatography of flufenamic acid in rat plasma.
A simple high-performance liquid chromatographic (HPLC) method for the determination of flufenamic acid in rat plasma is described. After liquid-liquid extraction, the drug is separated by HPLC on a 5-microns octadecylsilica column (Nucleosil C18) with ultraviolet detection at 280 nm. Linear calibration graphs for flufenamic acid were constructed from 0.5 to 15 micrograms/ml. The method has been applied to a pharmacokinetic study in animals.
Cerretani
D
D
Institute of Pharmacology, University of Siena, Italy.
Micheli
L
L
Fiaschi
A I
AI
Giorgi
G
G
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005439:Flufenamic Acid / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D012680:Sensitivity and Specificity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005439:Flufenamic Acid