Expression of markers for both neuronal and glial cells in human amniotic epithelial cells.
Human amniotic epithelial (HAE) cells are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. We attempted to detect various developmental antigens specific to neural cells by immunocytochemical methods. The cultured HAE cells displayed positive immunoreactivity to RC1, vimentin, A2B5, neurofilament proteins, microtubule-associated protein 2 (MAP2) and MAP2 kinase. In addition, the cells also demonstrated immunoreactivity to glial fibrillary acidic protein, CNPase, myelin basic protein and galactocerebroside. The appearance rate of positive cells was more than 50% in cells positive to RC1, A2B5, vimentin or neuronal markers, and 20-30% to glial cell markers. Double staining showed the heterogeneous appearance of oligodendrocyte lineage cells. These data indicate that HAE cells may have the putative multipotentiality of neurons, astrocytes and oligodendrocytes.
Sakuragawa
N
N
Department of Inherited Metabolic Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187, Japan.
Thangavel
R
R
Mizuguchi
M
M
Hirasawa
M
M
Kamo
I
I
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis; D000650:Amnion / Q000166:cytology*; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D005699:Galactosylceramides / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000032:analysis; D004676:Myelin Basic Protein / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000096:biosynthesis; D016900:Neurofilament Proteins / Q000032:analysis; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D015415:Biomarkers; D005699:Galactosylceramides; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D014746:Vimentin; C105547:MARK1 protein, human; D017346:Protein Serine-Threonine Kinases; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases