Changes in GST-isoenzyme pattern of some organs of sheep exposed to different levels of pollution.
GST isonzyme patterns were studied in the cytosolic fraction of liver, kidney and lung of sheep exposed to industrial metal pollutants and compared with those of control animals. The methodology included the determination of enzymatic activities with several subunit-specific substrates (DCNB, NPB, EPNP and EA) and Western blotting using antibodies to specific rat GST subunits 1, 8 (alpha class), 3 (mu class) and 7 (pi class). In liver and lung, crossed reactivities with subunits 1 and 3 were absent in the controls but were present in exposed animals. Just the opposite result was obtained for subunit 8 crossed reactivity that was only in the control animals. In the kidney, crossed reactivities towards subunits 3 and 8 were absent and crossed reactivity equivalent to subunit 7 was present in all animals, and equivalent to subunit 1 was weakly induced in exposed animals. A 3.3-fold increase in the activity with NPB detected in the kidneys of exposed animals points to the induction of a theta class isoenzymes. Clear increases were found in the livers of exposed animals in the activities with CDNB (1.8-fold), DCNB (2.6-fold) and EPNP (2.1-fold), but no differences were found in the lungs with any of the substrates. The GST isoenzyme pattern of liver and lung could be, in principle, a useful biomarker of exposure to environmental pollution in sheep.
Lenártová
V
V
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine, Kosice, Slovakia.
Holovská
K
K
Martinez-Lara
E
E
López-Barea
J
J
Barcena
J A
JA
Rosival
I
I
D000818:Animals; D015153:Blotting, Western; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants / Q000633:toxicity; D004787:Environmental Pollution / Q000009:adverse effects*; D005260:Female; D005982:Glutathione Transferase / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D008670:Metals / Q000633:toxicity; D012756:Sheep; D018154:Slovakia
D004785:Environmental Pollutants; D007527:Isoenzymes; D008670:Metals; D005982:Glutathione Transferase