Immunoglobulins of camel (Camelus dromedarius) colostrum.
Immunoglobulins G, M and A were identified in dromedary camel colostra by acid precipitation, gel filtration and fast-protein liquid chromatography (ion exchange). Heavy and light chains were identified by polyacrylamide gel electrophoresis and Western blotting. IgG subclasses (IgG1, IgG2 and IgG3) were isolated by DEAE ion-exchange chromatography and shown to have different electrophoretic mobilities. Cross-reactivity of camel IgA with IgA of other species was determined by enzyme-linked immunosorbent assay (ELISA). Most of the immunoglobulin content was IgG, but a molecule identifiable as IgA was detected and purified. It would appear that in the camel, as in cattle, IgG is the major secretory immunoglobulin of colostrum.
Azwai
S M
SM
Department of Veterinary Pathology, University of Liverpool, UK.
Carter
S D
SD
Woldehiwet
Z
Z
D000818:Animals; D002162:Camelus / Q000276:immunology*; D002415:Cats; D002845:Chromatography / Q000379:methods; D003126:Colostrum / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006736:Horses / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000302:isolation & purification; D007136:Immunoglobulins / Q000032:analysis* / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D013045:Species Specificity; D013552:Swine / Q000276:immunology
D007070:Immunoglobulin A; D007136:Immunoglobulins