Nucleosome assembly: the CAF and the HAT.
Recent data argue strongly that a protein complex termed chromatin assembly factor-1 (CAF-I) plays a major role in de novo nucleosome assembly during DNA replication. Human CAF-I deposits newly synthesized, acetylated histones onto replicated DNA in vitro and localizes to sites of DNA replication in S-phase cells. Specific lysines of the histones used for nucleosome assembly are acetylated; in the past year the first gene encoding a histone acetyltransferase was cloned. However, mechanistic links between histone acetylation and nucleosome assembly have not been established in vivo or in vitro.
Kaufman
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Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. pkauf@mendel.berkeley.edu
D000123:Acetyltransferases / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry* / Q000201:enzymology; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051548:Histone Acetyltransferases; D006801:Humans; D009707:Nucleosomes / Q000737:chemistry* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases