High-performance liquid chromatographic assay of propofol in human and rat plasma and fourteen rat tissues using electrochemical detection.
This paper describes a sensitive HPLC-electrochemical detection analytical method for determining the concentration of the intravenous anesthetic, propofol, in human or rat plasma or serum and a variety of rat tissues. Internal standard and drug are extracted from serum or plasma and other tissues with pentane. 2,6-tert.-Butylmethylphenol is used as internal standard. It includes a novel steam distillation procedure for separating the highly lipophilic propofol from skin and fat. The plasma/serum assay has a precision of 1-4% (C.V.) in the range 10 ng/ml to 1 microgram/ml and permits the assay of assay of 5 ng/ml from 0.1 ml of plasma/serum. The tissue procedure allows the estimation of 50 ng/g in 0.1 g of tissue for most of the major organs with less than 2% (C.V.) precision. This assay was used to measure propofol concentrations in plasma/serum and tissue samples in support of a project to develop a physiological pharmacokinetic model for propofol in the rat.
Dowrie
R H
RH
Department of Anesthesia, Stanford University School of Medicine, CA 94305, USA.
Ebling
W F
WF
Mandema
J W
JW
Stanski
D R
DR
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004563:Electrochemistry; D006801:Humans; D009928:Organ Specificity; D015742:Propofol / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
D015742:Propofol