title: SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats.
abstract: Aging of rats results in slower activities of calcium transport by cardiac calcium adenosinetriphosphatase (ATPase) of the sarcoplasmic reticulum (SR) and mitochondrial cytochrome oxidase (COX). These enzyme activities are faster after exercise training of previously sedentary old rats. Our purpose was to determine whether the expression of the genes encoding SR calcium ATPase (SERCA2a) or COX is altered by exercise training. Old (24-mo-old) male Fischer 344 rats were assigned to SO (sedentary old) or EO (exercised old) groups and compared with younger (12-mo-old) sedentary rats (SM). EO rats were trained on a treadmill for 8-10 wk. SERCA2a and COX mRNAs were lower (P < 0.05) in SO compared with SM and EO, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and cardiac alpha-actin mRNAs were similar across groups. The immunoreactive protein contents of cardiac calcium ATPase, cytochrome c, sarcomeric actin, and GAPDH followed the changes, when observed, in mRNA contents. Thus pretranslational mechanisms may be modified in some genes during aging and exercise training of previously sedentary old rats.
authors: Department of Pharmacological and Pharmaceutical Sciences, University of Houston 77204-5515, Texas, USA.
authors: C A
authors: CA
authors: Tate
authors: T
authors: T
authors: Helgason
authors: M F
authors: MF
authors: Hyek
authors: R P
authors: RP
authors: McBride
authors: M
authors: M
authors: Chen
authors: M A
authors: MA
authors: Richardson
authors: G E
authors: GE
authors: Taffet
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D008297:Male; D008929:Mitochondria, Heart / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D000252:Calcium-Transporting ATPases