Enhanced vesiculation exacerbates complement-dependent hemolysis in glucose-6-phosphate dehydrogenase deficient red blood cells.
Glucose-6-phosphate dehydrogenase (G6PD) deficient red blood cells (RBCs) are known to be more susceptible to oxidant-induced hemolysis. Erythrocytes from G6PD-deficient individuals are significantly more susceptible to Ca(2+)-induced vesiculation than normal control cells. The enhanced susceptibility of G6PD-deficient RBCs to Ca(2+)-induced vesiculation is not due to ATP depletion. The remnant G6PD-deficient RBCs following vesiculation are more sensitive to complement-mediated hemolysis than control normal RBCs. A strong positive correlation exists between the level of Ca(2+)-induced vesiculation and the extent of complement mediated hemolysis.
Tsai
K J
KJ
Chang Gung College of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
Shih
L Y
LY
Hung
I J
IJ
Huang
S W
SW
Wei
J S
JS
Liu
T Z
TZ
Chiu
D T
DT
D002118:Calcium / Q000378:metabolism; D003165:Complement System Proteins / Q000502:physiology*; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D011770:Pyruvate Kinase / Q000378:metabolism
D003165:Complement System Proteins; D005954:Glucosephosphate Dehydrogenase; D011770:Pyruvate Kinase; D002118:Calcium