Evidence to support the peripheral branching of primary afferent C-fibres in the rat: an in vitro intracellular electrophysiological study.
Intracellular voltage recordings were made in vitro at 36.5 +/- 1 degrees C from 35 rat lumbar dorsal root ganglion (DRG) neurones with a peripheral conduction velocity (CV) in the C-fibre range (0.3-2.2 m/s). The peripheral nerve (PN) was stimulated in one of three different ways, each delivering single stimuli (0.1-1 ms duration, 2-3-times threshold; maximum 50 V) at a low frequency (0.3 Hz). With each of the three stimulation methods used here a similar proportion of cells (approximately 30%) showed changes, either an abrupt latency change or a soma invasion by two action potentials (APs). Both of these changes were consistent with branching of primary afferent C-fibres in the PN.
McCarthy
P W
PW
Anglo-European College of Chiropractic, Bournemouth, Dorset, UK.
Prabhakar
E
E
Lawson
S N
SN
D000344:Afferent Pathways / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000502:physiology; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D066298:In Vitro Techniques; D008161:Lumbosacral Region; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure*; D009431:Neural Conduction / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar