title: Antiepithelial cell antibodies do not impair paediatric renal allograft survival but appear to be associated with acute viral infections.
abstract: There is a reported association between antiepithelial cell (AEC) antibodies and increased renal allograft loss in paediatric recipients. Our unit experienced a dramatic fall in 1-year graft survival so we undertook a study to determine if AEC antibodies could account for such losses. We also studied healthy children and adults as well as a group of individuals with serologically proven viral infection in an attempt to determine the prevalence and possible aetiology of these antibodies. Sera were screened for AEC antibodies in a microcytotoxicity test using a lung epithelial cell line (A549) as target. The prevalence of these antibodies in our paediatric recipients was similar to that reported elsewhere but we found no correlation between the presence of AEC antibody and allograft loss. Within the control populations, we found the antibody was more prevalent in children than in adults (p < 0.0001). We also found a strong age banding pattern, with antibody being present in 50% of children under 10 years and declining with increasing age, so that by the age of 16 years the seroprevalence was similar to that found in our adults. However, AEC antibody had a significantly higher prevalence in individuals with active viral infection than in our healthy control groups (p = 0.00003). A positive association was noted between rubella and respiratory syncytial virus and AEC antibody presence and a negative association with varicella zoster. We conclude that AEC antibodies do not correlate with increased paediatric renal allograft loss but appear to be linked to certain viral infections.
authors: Beaumont Hospital, Dublin, Ireland.
authors: S E
authors: SE
authors: Spencer
authors: N
authors: N
authors: McCarthy
authors: B
authors: B
authors: Hannigan
authors: D
authors: D
authors: Gill
authors: M R
authors: MR
authors: Taylor
authors: D
authors: D
authors: Murphy
authors: J J
authors: JJ
authors: Walshe
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D002644:Chickenpox / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology; D014645:Herpesvirus 3, Human; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology; D012409:Rubella / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; C044203:anti-IgM