Influence of a rat kidney perfusion using the anti-MHC class II monoclonal antibody and reduced dose of 15-deoxyspergualine on the allogeneic graft survival.
One of the main factors inducing rejection of the allogenic graft are the donor MHC-class II antigens. In this study, the allogenic rat renal graft survival after the blockage of MHC-class II positive cells was analyzed and compared with the effectiveness of the recipient treatment with 15-deoxyspergualine (15-DOS). It was found that the DA (RT1 a) rat kidney perfusion with the anti-MHC class II monoclonal antibody (MoAb 29A1--Kiel) allowed to prolong survival of the graft in the LEW (RT1 1) recipient (9.6 +/- 0.8 vs. 7.7 +/- 0.5 days). Our another study demonstrated that the 14 day treatment of the LEW recipient with 15-DOS at the dose of 0.5 mg/kg body weight can induce tolerance to the grafted kidney from the DA strain. The dose of 0.2 mg/kg body weight of 15-DOS prolonged the graft survival only to a small extend (16.5 +/- 0.5 days). In contrast, the combination of the graft pretreatment with MoAb 29A1 with the application of the reduced dose of 15-DOS to the LEW recipient allowed to further prolong the graft survival (97.4 +/- 59.0 days, n = 5). In 3 cases, the long-time (close to 150 days) graft survival was obtained. The above presented results suggest that the blockade of the MHC-class II antigens can reduce the immunogenicity of the graft. Although this procedure was not sufficient to induce tolerance, it allowed to minimize the immunosuppressive treatment.
Oko
A
A
Department of Nephrology, Medical Academy, Poznań, Poland.
Krzymański
M
M
Deja
A
A
Ulrichs
K
K
Waaga
A M
AM
Müller-Ruchholtz
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006085:Graft Survival / Q000187:drug effects*; D006146:Guanidines / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D010477:Perfusion; D051381:Rats; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D006146:Guanidines; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; C037258:gusperimus