Platelet deposition and fibrinogen binding on surfaces coated with heparin or friction-reducing polymers.
The blood-contacting properties of polyethylene coated with a lubricious hydrophilic coating; an uncoated polyethylene; or a photoheparin-treated polyethylene-negative control sample were compared by measuring fibrinogen adsorption, antifibrinogen binding, and platelet attachment from human plasma. The polyethylene surfaces coated with a hydrophilic polymer were found to be similar to surfaces coated with heparin. Fibrinogen adsorption on the hydrophilic coating was 60% lower than adsorption on either the uncoated or heparin-coated polyethylene samples. Antifibrinogen binding from buffer to the hydrophilic coating was also reduced more than 85% from binding to uncoated polyethylene samples. Both the hydrophilic coating and heparin coating showed a reduction in platelet attachment by a factor of 100 over the uncoated sample as well as significantly reduced platelet activation.
Anderson
A B
AB
BSI Corporation, Eden Prairie, Minn, USA.
Tran
T H
TH
Hamilton
M J
MJ
Chudzik
S J
SJ
Hastings
B P
BP
Melchior
M J
MJ
Hergenrother
R W
RW
D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000911:Antibodies, Monoclonal; D002021:Buffers; D005340:Fibrinogen / Q000737:chemistry*; D017276:Friction; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010949:Plasma; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011095:Polyethylenes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry; D011485:Protein Binding; D013501:Surface-Active Agents / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D002021:Buffers; D005340:Fibrinogen; D006493:Heparin; D011095:Polyethylenes; D011108:Polymers; D011205:Povidone; D013501:Surface-Active Agents; D000099166:Polyacrylamides