Human CNTF and related cytokines: effects on DRG neurone survival.
Ciliary neurotrophic factor (CNTF), leukaemia inhibitory factor (LIF), oncostatin M (OSM), interleukin-6 (IL-6), and interleukin-11 (IL-11) are structurally and functionally related cytokines. We compared their survival-promoting activities on embryonic chick and newborn rat dorsal root ganglion (DRG) neurones. Human CNTF showed the well known trophic effect on both chick and rat DRG neurones. Human and murine LIF and, at unphysiologically high doses, human OSM were trophic for rat neurones, but failed to promote chick DRG cell survival. Human IL-11, murine IL-6 and human IL-6 did not improve chick or rat DRG neurone survival; soluble human IL-6 receptor alpha did not increase sensitivity to human IL-6. Thus, human CNTF as well as murine and human LIF had special neurotrophic properties compared with other related cytokines.
Simon
R
R
Institute of Neuropathology, Technical University, Aachen, Germany.
Thier
M
M
Krüttgen
A
A
Rose-John
S
S
Weiergräber
O
O
Heinrich
P C
PC
Schröder
J M
JM
Weis
J
J
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001681:Biological Assay; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines / Q000494:pharmacology*; D004357:Drug Synergism; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018123:Receptors, Interleukin / Q000378:metabolism; D019947:Receptors, Interleukin-6; D013045:Species Specificity
D015703:Antigens, CD; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6