[Detection of minimal residual leukemia using polymerase chain reaction method].
Until recently, it was impossible to identify leukemia cells making up less than 1% of a bone marrow sample, which is designated as minimal residual disease (MRD). Owing to the development of the polymerase chain reaction (PCR) MRD can be detected at the 10(-5) level by amplifying the leukemia-specific DNA rearrangement (BCR/ABL, PML/RARA etc.) or clone-specific DNA sequences (IgH chain CDR-III etc.). Here, our studies on MRD of acute lymphoblastic leukemia and acute promyelocytic leukemia are presented, and their technical problems and clinical significance are discussed.
Naoe
T
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Nagoya University Branch Hospital.
D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis*; D018365:Neoplasm, Residual / Q000175:diagnosis; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm