Assignment of markers by using polymerase chain reaction on pools of swine flow-sorted chromosomes.
Gene chromosomal assignment can be realized not only by somatic hybrid panels but also by spot-blot hybridization or polymerase chain reaction (PCR) of flow-sorted chromosomes. We propose a swine chromosome assignment strategy by PCR amplification on pooled chromosomal DNA, which allows assignment despite possible chromosomal contamination during sorting. Each pool contains three different chromosomes, each chromosome being present in one or two pools. We present concordant results obtained for eight markers already mapped to different swine chromosomes and we assign the somatostatin gene to chromosome 13, a new marker in the pig genome.
Riquet
J
J
Laboratoire de Génétique Cellulaire, INRA BP27, Castanet Tolosan, France.
Schmitz
A
A
Cruzel
J
J
Mulsant
P
P
Milan
D
D
Gellin
J
J
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry; D005819:Genetic Markers; D007621:Karyotyping; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013004:Somatostatin / Q000235:genetics; D013552:Swine / Q000235:genetics*
D005819:Genetic Markers; D013004:Somatostatin