Recombinant human c-Mpl ligand is not a direct stimulator of proplatelet formation in mature human megakaryocytes.
To evaluate the effect of the c-Mpl ligand on platelet production by megakaryocytes, we investigated proplatelet formation in isolated human megakaryocytes cultured in serum-free medium, with or without the c-Mpl ligand, interleukin-6 and erythropoietin. When interleukin-6 was added to the culture medium, the percentage of megakaryocytes displaying proplatelets was approximately 1.5-fold the control value; whereas, in the presence of the c-Mpl ligand, the percentage of megakaryocytes displaying proplatelets decreased in a dose-dependent manner and did not increase compared to control values at any dose tested. However, the viability of megakaryocytes after a 4 d culture in the presence of the c-Mpl ligand was significantly higher than that of the cells cultured without it. The c-Mpl ligand did not stimulate the proplatelet formation in megakaryocytes in vitro.
Ito
T
T
Third Department of Internal Medicine, Iwate Medical University, Japan.
Ishida
Y
Y
Kashiwagi
R
R
Kuriya
S
S
D001792:Blood Platelets / Q000187:drug effects*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D008533:Megakaryocytes / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D015850:Interleukin-6; D011994:Recombinant Proteins; D013926:Thrombopoietin