Characterisation of murine monoclonal antibodies recognising opsonic, mouse-protective, chaining and mucosally relevant epitopes on the M protein of Streptococcus equi subspecies equi.
Six hybridomas secreting murine monoclonal antibodies (mAbs) specific for the M protein of Streptococcus equi subspecies equi were characterised. The mAbs recognised the major 41 and 46 kDa fragments of M protein in an acid extract of S equi and the 56 and 58 kDa dimer of the native molecule in a mutanolysin extract, but did not react with recombinant M-like protein of S equi subspecies zooepidemicus. One mAb (2A10) showed strong opsonic activity for S equi and protected mice against an experimental challenge with virulent S equi. Two other mAbs were mouse-protective but not opsonic. All the mAbs elicited a strong chaining response from S equi, but had only a weak chaining effect on a strain of S equi (19) that expressed only 4 per cent of the normal amount of M protein. Antibodies in nasopharyngeal mucus of horses recently recovered from strangles were inhibited to different extents by each mAb. These different functional behaviours and the result of inhibition ELISAs suggest that the M protein of S equi carries multiple epitopes.
Timoney
J F
JF
Department of Veterinary Science, University of Kentucky, Lexington 40546-0099, USA.
Guan
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology*; D002352:Carrier Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006734:Horse Diseases; D006736:Horses; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D018502:Streptococcus equi / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D000939:Epitopes; C014689:streptococcal M protein