title: Human cord blood-derived large myeloid cells: spontaneous shape change and chemotactic movement.
abstract: We have demonstrated two aspects of the movement of large myeloid cells derived from cord blood mononuclear cells after culture in RPMI 1640 and 10% fetal calf serum for eight days. At this time cells assume various shapes including round, oval, veiled, elongated and dendritic. Interestingly, these shapes were not static. Time-lapse recording demonstrated that individual cells change shape quite dramatically over four hours. Some of the veils showed a great deal of activity, some elongated cells shrank into rounded forms, and rounded cells stretched out into dendritic cells or elongated forms. We have previously shown that the number of N-formyl-methionine-leucyl-phenylalanine receptors on the cell surface increase with culture time up to eight days. Furthermore, a rise in Ca2+ was observed in response to 1 mumol/L N-formyl-methionine-leucyl- phenylalanine (chemotactic peptide) at the single cell level. In this paper we show the motility of cord blood-derived myeloid cells towards a chemotactic peptide concentration gradient leaking from a micropipette.
authors: Department of Surgery, University of Wales College of Medicine, Health Park, Cardiff, UK.
authors: G M
authors: GM
authors: Roberts
authors: I
authors: I
authors: Laffafian
mesh_terms: D001854:Bone Marrow Cells; D048429:Cell Size; D002478:Cells, Cultured; D002633:Chemotaxis; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D014743:Videotape Recording