Antisense oligodeoxynucleotide attenuates in vivo expression of c-fos in the paraventricular hypothalamic nucleus of the rat brain.
The proto-oncogene, c-fos, is expressed in neurons in response to stimulation by growth factors, neurotransmitters, and immunocytokines. In order to determine whether interleukin-1 beta (IL-1 beta)-induced Fos protein expression in the paraventricular hypothalamic nucleus (PVN) can be reduced or eliminated by a c-fos antisense oligodeoxynucleotide, rats were injected with c-fos antisense and Fos expression was examined immunocytochemically. Our results indicate that c-fos antisense oligodeoxynucleotide treatment decreases the density of Fos immuno-labeled nuclei in the PVN following IL-1 beta administration. These data suggest that, with the use of antisense technology, it may be possible to determine the role of proto-oncogene proteins in specific brain areas.
Quercia
S
S
Department of Biology, Seton Hall University, South Orange, NJ 07079, USA.
Chang
S L
SL
D000818:Animals; D001921:Brain / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D016376:Oligonucleotides, Antisense; D016760:Proto-Oncogene Proteins c-fos