Facile analysis of ribonucleotides in D,L-alpha-difluoromethylornithine-treated mouse lymphoid cells by high-performance anion-exchange column chromatography.
Using high-performance liquid chromatography with the strong anion-exchange resin column CDR-10 for analysis of ribonucleotides, the effects of D,L-alpha-difluoromethylornithine (DFMO) on ribonucleotides of mouse leukemic lymphoid cells SC-1 have been studied. More than 16 nucleoside mono-, di and triphosphates, and unknown peaks were clearly separated and measured without increase in baseline rise using the gradient systems of phosphate buffer. The ATP level in DFMO-treated SC-1 cells was reduced, but was reversed by exogenous putrescine. These facts may suggest that polyamine depletion by DFMO during a short time (6 h) caused mitochondrial damage in mammalian cells.
Cho
S C
SC
Department of Biology, School of Health Sciences, Fujita Health University, Aichi-ken, Japan.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000518:Eflornithine / Q000494:pharmacology*; D007700:Kinetics; D007942:Leukemia, Experimental; D008214:Lymphocytes / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011700:Putrescine / Q000494:pharmacology; D012265:Ribonucleotides / Q000032:analysis*; D014407:Tumor Cells, Cultured
D012265:Ribonucleotides; D000255:Adenosine Triphosphate; D011700:Putrescine; D000518:Eflornithine