title: Development of a model system to compare cell separation methods for the isolation of fetal cells from maternal blood.
abstract: Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescence-activated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a "model' male fetal cell mixed into female peripheral blood mononuclear cells. Fetal cell yields and purities were assayed by a quantitative polymerase chain reaction (qPCR) using chromosomes Y- and 7-specific sequences. Fetal cell recovery was investigated by selection of CD71+ cells or depletion of CD45+ cells. Our data demonstrated variation in fetal cell recovery for all methods tested, although CD71+ selection by FACS gave the best and most consistent results.
authors: New England Medical Center, Boston, Massachusetts, USA.
authors: D W
authors: DW
authors: Bianchi
authors: K W
authors: KW
authors: Klinger
authors: T J
authors: TJ
authors: Vadnais
authors: M A
authors: MA
authors: Demaria
authors: A P
authors: AP
authors: Shuber
authors: J
authors: J
authors: Skoletsky
authors: P
authors: P
authors: Midura
authors: M
authors: M
authors: Diriso
authors: C
authors: C
authors: Pelletier
authors: M
authors: M
authors: Genova
authors: M S
authors: MS
authors: Erikson
authors: J M
authors: JM
authors: Williams
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000097:blood*; D011296:Prenatal Diagnosis / Q000379:methods*; D011990:Receptors, Transferrin; D015203:Reproducibility of Results
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D011990:Receptors, Transferrin; D017493:Leukocyte Common Antigens