Protection of isolated lung from reperfusion injuries by rinsing with high colloidal osmotic solution with deferoaxmine.
We examined the efficacy of rinsing isolated lungs subjected to prolonged hypothermic storage with a high colloidal osmotic pressure solution prior to ex vivo blood reperfusion in order to preserve physiologic functions, suppress peroxidation of mitochondrial membranes, and inhibit infiltration of neutrophils. Isolated rabbit lungs were flushed with a Rinse-1 solution (289 mOsm/kg H2O) to remove remaining blood and immersed in physiologic saline at 8 degrees C for 24 hr. The control group received blood reperfusion immediately after storage; the Rinse-1 group was rinsed with Rinse-1 solution before blood reperfusion and the Rinse-2 group with Rinse-2 solution (312 mOsm/kg H20) including deferoxamine. Reperfused blood was passed through an artificial membranous lung to reduce oxygen tension (PO2) to the venous level, and time-dependent changes in airway pressure (AWP), pulmonary artery pressure (PAP), and PO2, as a measure of gas-exchange capability were examined. We estimated the lipid peroxide level in mitochondrial membranes as thiobarbituric acid-reactive substances (TBARS), i.e., malonedialdehyde, and neutrophil infiltration into lung tissue by measuring myeloperoxidase activity after 60 min of blood reperfusion. The PO2 was significantly higher in both rinsed groups compared with the control, while neither AWP nor PAP was significantly different in the three treatment groups. Both mitochondrial TBARS and myeloperoxidase activity were significantly higher in the control group compared with either rinsed group. These results indicate that rinsing stored lungs with a solution of high colloidal osmotic pressure prior to blood reperfusion was effective in preserving physiologic function and inhibiting neutrophil infiltration. Addition of deferoxamine was markedly effective in reducing TBARS formation and lessening reperfusion injury of stored lungs.
Shibuya
K
K
Second Department of Surgery, Miyazaki Medical College, Japan.
Edagawa
M
M
Takenaka
H
H
Matsuzaki
Y
Y
Shibata
K I
KI
Onitsuka
T
T
Koga
Y
Y
Hamada
M
M
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D003102:Colloids; D003676:Deferoxamine / Q000737:chemistry / Q000627:therapeutic use*; D007036:Hypothermia, Induced; D008054:Lipid Peroxides / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000187:drug effects / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D009994:Osmolar Concentration; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D009195:Peroxidase / Q000378:metabolism; D011651:Pulmonary Artery / Q000502:physiology; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D012119:Respiration / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D003102:Colloids; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D009195:Peroxidase; D003676:Deferoxamine; D010100:Oxygen