[Inhibitory effect of Kanpo-medicines: saiboku-to, syouseryu-to, sairei-to on Dermatophagoides farinae antigen-induced IL2 responsiveness in lymphocytes from patients with bronchial asthma and their comparison].
We had previously reported that antigen-induced IL2 responsiveness by lymphocytes can be used to identify etiological allergens and to monitor the clinical activity in atopic diseases. The effect of Kanpo-Medicines; Saiboku-to, Syoseiryu-to, Sairei-to, antiallergic agents, on Dermatophagoides farinae (Df) antigen-induced interleukin 2 (IL2) responsiveness from patients with bronchial asthma was studied and compared among 3 Kanpo-medicines. Allergen-sensitized patient mononuclear cells pretreated with 30-30,000 ng/ml doses of Saiboku-to for 16 hours failed to induce responsiveness to IL2 on stimulation with Df antigen in 8 patients out of 11 (73%). The cells treated with 30 micrograms/ml of Syoseiryu-to also failed to introduce the response in 6 out of 10 (60%) with less frequency compared with Saiboku-to. Sairei-to also suppressed the response on stimulation with a 10 micrograms/ml dose alone in 1 x 10(-3)-1 x 10(-4)ng/ml doses examined in 6 patients out of 17 (35%) with much less frequency and % inhibition. Also Saiboku-to and Sairei-to not Syoseiryu-to inhibited purified protein derivatives (PPD)-induced IL2 responsiveness. However. These agents failed to suppress the Con A-induced IL2 responsiveness. Antigen-presenting adherent cells were more susceptible to any Kanpo-medicines studied rather than IL2-responding T cells except Sairei-to. These results indicated that Kanpo-medicines studied have a weak immuno-suppressive property resulting in inhibiting antigen-induced IL2-responsiveness. Suppressive effects of each Kanpo-medicine seemed to depend on combinations and doses of their elements (Syouyaku). Decreased suppressive effect of higher doses of any Kanpo-medicine was likely to be mitogenic effects of each element on T cells.
Noma
T
T
Department of Pediatrics, Saitama Medical School, Japan.
Nakajima
T
T
Kawano
Y
Y
Yoshizawa
I
I
Kabuki
T
T
Baba
M
M
D000293:Adolescent; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008925:Mites / Q000276:immunology*
D000941:Antigens; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2