[The effects of single administration of kainic acid on cellular signal transmission pathway and susceptibility to seizure].
In this study, Sprague-Dawley (S.D.) rats were pretreated with kainic acid (KA, 10 mg/kg, i. sc.) 72 days before either deep prepyriform cortex (DPC) kindling or injection of the same dose of KA. The results showed that such pretreatment accelerated the process of kindling, and the KA-induced seizures appeared more severe in behaviours. c-Fos-immunoreactivity (c-Fos-ir) was used to demonstrate the neuronal activity in central nervous system during the provocative dose of KA-induced seizure, as comparing with the control which received normal saline of KA 72 days ago. The results indicated that, the transmission pathways in two groups were just the same; but the time courses of c-fos and c-jun expression in KA pretreated group were accelerated asynchronously; and the synthesis of the proenkephalin mRNA in the Ent was apparently up-regulated. It is suggested that, the long-lasting increase in susceptibility to seizure induced by a single administration of KA may be related to the acceleration of the expression of c-fos/jun and the early onset of the expression of proenkephalin mRNA.
Gao
X
X
Department of Physiology, Dalian Medical University.
Zhang
W Q
WQ
Hong
J S
JS
D000818:Animals; D004198:Disease Susceptibility; D004745:Enkephalins / Q000096:biosynthesis; D004827:Epilepsy / Q000235:genetics*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D016758:Genes, jun; D007608:Kainic Acid / Q000494:pharmacology*; D007696:Kindling, Neurologic; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*
D004745:Enkephalins; D018690:Excitatory Amino Acid Agonists; D011498:Protein Precursors; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; C029992:proenkephalin; D007608:Kainic Acid