Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana.
A family of calcium-responsive protein kinases is abundant in plant cell extracts but has not been identified in animals and fungi. These enzymes have a unique structure consisting of a protein kinase catalytic domain fused to carboxy-terminal autoregulatory and calmodulin-like domains. In this report, we present the amino acid sequences for eight new Arabidopsis cDNA clones encoding isoforms of this enzyme. Three isoforms were expressed as fusion proteins in Escherichia coli and exhibited calcium-stimulated protein kinase activity. We propose CPK as the gene designation for this family of enzymes and describe a phylogenetic analysis for all known isoforms.
Hrabak
E M
EM
Department of Plant Biology, University of New Hampshire, Durham 03824, USA.
Dickmann
L J
LJ
Satterlee
J S
JS
Sussman
M R
MR
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic
D018076:DNA, Complementary; D007527:Isoenzymes; D017871:Calcium-Calmodulin-Dependent Protein Kinases