Liposome-mediated NGF gene transfection increases ChAT activity in CNS cell cultures.
Liposome-mediated NGF transfection has been shown to increase the expression and secretion of NGF in primary rat septo-hippocampal cell cultures. Here we report that along with increased NGF expression, the activity of choline acetyltransferase, the synthetic enzyme for acetylcholine, is increased by 18% within 2 days, by 41% within 4 days and by 32% within 8 days after NGF gene transfection in septo-hippocampal cell cultures. This result further confirms that biologically active NGF is functionally expressed in septo-hippocampal cells when transfected with cDNA for NGF completed with liposomes.
Le
W
W
Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
Yang
K
K
Whitson
J S
JS
Clifton
G L
GL
Hayes
R L
RL
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008081:Liposomes; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection / Q000235:genetics*
D008081:Liposomes; D009414:Nerve Growth Factors; D002795:Choline O-Acetyltransferase