A plasmocyte selective monoclonal antibody (B-B4) recognizes syndecan-1.
We developed a new monoclonal antibody. B-B4, which specifically identifies human plasma cells. It strongly reacts with all multiple myeloma cell lines and with malignant plasma cells of all tumour samples of the multiple myeloma patients tested. B-B4 does not react with any peripheral blood, bone marrow or tonsil cells. Cloning of the B-B4 antigen reveals that the monoclonal antibody recognizes syndecan-1. It appears that the monoclonal antibody B-B4 is a suitable marker for human plasmocyte identification among haemopoietic cells and a useful probe for the diagnosis of haematological malignancies. Furthermore, this monoclonal antibody can be used for depletions prior to CD34 grafting.
Wijdenes
J
J
Diaclone, Besançon, France.
Vooijs
W C
WC
Clément
C
C
Post
J
J
Morard
F
F
Vita
N
N
Laurent
P
P
Sun
R X
RX
Klein
B
B
Dore
J M
JM
D000911:Antibodies, Monoclonal / Q000276:immunology*; D014408:Biomarkers, Tumor; D001773:Blood Cells / Q000276:immunology; D015153:Blotting, Western; D001853:Bone Marrow / Q000276:immunology; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000276:immunology; D014066:Palatine Tonsil / Q000276:immunology; D010950:Plasma Cells; D011509:Proteoglycans / Q000276:immunology*; D053668:Syndecan-1; D053667:Syndecans; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans