title: TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor shedding during detachment.
abstract: T cell recognition of foreign Ag/MHC class II complexes is sensitive down to approximately 100 complexes per cell or approximately 0.2 complexes/micron2. To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. Adhesion of 3A9 cells required a superphysiologic density of the MHC class II/peptide complex and was partly dependent on CD4; cells adhered but did not crawl. No adhesion was observed to bilayers containing MHC class II molecules without the lysozyme peptide. Activated 3A9 cells adhered and crawled on bilayers containing ICAM-1. The physical strength of contacts was tested with fluid shear. 3A9 cells adherent to bilayers containing MHC class II/peptide complexes shed their contact, which remained on the substrate and contained TCR. In contrast, 3A9 cells peeled from the ICAM-1 bilayer, and held firmly on LFA-1 bilayers; in a manner dependent on filamentous actin. When ICAM-1 and the MHC/peptide complexes were combined, the 3A9 cells adhered tightly and spread, but did not crawl, on the bilayers and TCR clustered at the center of the contact area. Physiologically, the TCR is unlikely to directly initiate adhesion. TCR clusters formed with the assistance of adhesion mechanisms may have to be shed to allow de-adhesion, and this may contribute to TCR down-regulation.
authors: Center for Immunology and Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors: M L
authors: ML
authors: Dustin
authors: J M
authors: JM
authors: Miller
authors: S
authors: S
authors: Ranganath
authors: D A
authors: DA
authors: Vignali
authors: N J
authors: NJ
authors: Viner
authors: C A
authors: CA
authors: Nelson
authors: E R
authors: ER
authors: Unanue
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D006825:Hybridomas / Q000378:metabolism / Q000502:physiology*; D008051:Lipid Bilayers / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism* / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*
chemical_list: D000949:Histocompatibility Antigens Class II; D008051:Lipid Bilayers; D010455:Peptides; D011948:Receptors, Antigen, T-Cell