Influence of chondroitinase on indirect hemagglutination titers and phagocytosis of Pasteurella multocida serogroups A, D and F.
Capsules of Pasteurella multocida serogroups A, D and F contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. In this study, capsules of serogroup A, D and F strains of P. multocida were depolymerized by enzyme treatment. Capsule depolymerization of serogroup D and F strains with chondroitinase increased indirect hemagglutination (IHA) test titers and enhanced phagocytosis by swine neutrophils. Capsule depolymerization of serogroup A strains with hyaluronidase increased IHA titers, but depolymerization with chondroitinase did not. When serogroup A strains were treated with a combination of chondroitinase and hyaluronidase, IHA test titers were lower than titers of the same strains treated with hyaluronidase alone. Combined enzyme treatment of serogroup D strains resulted in IHA test titers similar to those of chondroitinase treatment alone.
Rimler
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RB
Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA.
Register
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KB
Magyar
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T
Ackermann
M R
MR
D000818:Animals; D002417:Cattle; D002645:Chickens; D002810:Chondroitinases and Chondroitin Lyases; D005434:Flow Cytometry; D006025:Glycosaminoglycans; D006386:Hemagglutination Tests; D009504:Neutrophils / Q000382:microbiology / Q000502:physiology*; D016979:Pasteurella multocida / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial; D011817:Rabbits; D046048:Seals, Earless; D013552:Swine; D014422:Turkeys
D006025:Glycosaminoglycans; D011135:Polysaccharides, Bacterial; D002810:Chondroitinases and Chondroitin Lyases