Inhibition of platelet aggregation by endocardial endothelial cells.
We assessed the anti-platelet properties of endocardial endothelial cells (EECs) by measuring platelet aggregation after brief interaction with EECs isolated from the right ventricles of porcine hearts. Platelet aggregation in response to thrombin was significantly inhibited by brief incubation of platelet suspensions over EEC monolayers. Pretreatment of EECs with indomethacin restored platelet reaction but that with L-NAME and hemoglobin (Hb) did not. The PGI2 content of platelet suspensions after interaction with cultured EECs was significantly correlated with the inhibition of platelet aggregation. These results suggest that EECs inhibit platelet aggregation by releasing PGI2.
Nosaka
S
S
First Department of Surgery, Shimane Medical University, Japan.
Nakayama
K
K
Hashimoto
M
M
Sasaki
T
T
Gu
K
K
Saitoh
Y
Y
Kin
S
S
Yamauchi
M
M
Masumura
S
S
Tamura
K
K
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004699:Endocardium / Q000166:cytology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D011464:Epoprostenol / Q000378:metabolism; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D013552:Swine; D013917:Thrombin / Q000494:pharmacology
D010975:Platelet Aggregation Inhibitors; D001120:Arginine; D011464:Epoprostenol; D013917:Thrombin; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin