title: Collagen and glycosaminoglycans of Wharton's jelly and their alterations in EPH-gestosis.
abstract: Some prenatal pathological processes may be caused by biochemical and morphological alterations in the umbilical cord (UC). EPH-gestosis is the most common pregnancy-associated pathological process. For these reasons the role of collagen and glycos-aminoglycans (GAGs) of UC in pathobiochemistry of this syndrome seems to be important. We studied histology of extracellular matrix components, quantity, solubility and molecular polymorphism of collagen, proportional relationships between various types of collagen, the amounts of GAGs and proportional relationships between them in Wharton's jelly of control newborns delivered by healthy mothers and those delivered by mothers with EPH-gestosis. We found that Wharton's jelly is abundant in collagen and GAGs. This collagen is very insoluble and resistant to the action of depolymerizing agents (4% EDTA-Na2, pepsin). Types I, III and V collagens were isolated and quantified. Hyaluronic acid constitutes about 70%, whereas sulphated GAGs constitute about 30% of total GAGs. EPH-gestosis is accompanied by significant increase in sulphated GAGs: hyaluronic acid ratio. The EPH-gestosis-associated alterations in Wharton's jelly correspond to 'premature ageing' of this tissue.
authors: Department of Biochemistry, Medical Academy of Bialystok, Poland.
authors: E
authors: E
authors: Bańkowski
authors: K
authors: K
authors: Sobolewski
authors: L
authors: L
authors: Romanowicz
authors: L
authors: L
authors: Chyczewski
authors: S
authors: S
authors: Jaworski
mesh_terms: D000328:Adult; D003094:Collagen / Q000032:analysis* / Q000648:ultrastructure; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis* / Q000648:ultrastructure; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D011225:Pre-Eclampsia; D011247:Pregnancy; D012995:Solubility; D014470:Umbilical Cord / Q000737:chemistry* / Q000473:pathology
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen