Aluminosilicates enhance the infectivity of cytomegalovirus in urine using centrifugation-enhanced antigen detection technology.
Due to the inherent lability of CMV, necessary laboratory identification of this infectious agent is often compromised by a delay in specimen transport. Previous studies have addressed the phenomenon of infectivity enhancement/reduction in the rate of infectivity loss by the incorporation into various viral assay systems of trace concentrations of the adsorbents montmorillonite (bentonite [M]) or kaolinite (kaolin [K]). We extended these studies to the clinical setting to identify whether such aluminosilicates would effect an enhanced level of CMV infectivity. The shell vial assay-indirect immunofluorescent assay (SVA-IFA) was utilized in comparative testing throughout this study. The addition of trace concentrations of M or K to the SVA-IFA was found to enhance the infectivity of CMV in urine by 115 and 126%, respectively. The total CMV detection rate by SVA-IFA was 29% (30/105). Three of the 30 (10%) CMV positive specimens were detected only in shell vials which had been supplemented with K or M. Two specimens were isolation positive alone. The addition of K or M to shell vials immediately prior to the start of the SVA-IFA has the potential of (a), enhancing assay readability by increasing the number of fluorescent focus units per vial monolayer and (b), of detecting positive urine specimens with low viral titers which might otherwise not be identified using the conventional SVA-IFA procedure.
Lee
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Jane and Dayton Brown and Dayton T. Brown Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital-Cornell University Medical College, Manhasset, NY, USA.
Biondo
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Teichberg
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Lipson
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D000956:Antigens, Viral / Q000032:analysis*; D001546:Bentonite / Q000494:pharmacology*; D002498:Centrifugation; D003587:Cytomegalovirus / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007616:Kaolin / Q000494:pharmacology*; D008854:Microscopy, Electron; D014556:Urine / Q000821:virology*
D000956:Antigens, Viral; D001546:Bentonite; D007616:Kaolin