Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures.
Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly-L-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.
Bronstein
D M
DM
Laboratory of Environmental Neuroscience, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Perez-Otano
I
I
Sun
V
V
Mullis Sawin
S B
SB
Chan
J
J
Wu
G C
GC
Hudson
P M
PM
Kong
L Y
LY
Hong
J S
JS
McMillian
M K
MK
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000633:toxicity*; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D018696:Neuroprotective Agents / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D016627:Oxidopamine / Q000633:toxicity*; D011107:Polylysine; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009498:Neurotoxins; D011107:Polylysine; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase