T cells from rejected human kidney allografts respond to heat shock protein 72.
The immune response to heat shock proteins (hsps) is gaining more and more interest. Members of the 65 and the 70 kDa hsp families have been shown to be target molecules of the immune system in autoimmune diseases, in cancer immunity and recently in the rejection of rat heart allografts. It was therefore the aim of the present study to investigate whether T cells propagated from two rejected human renal allografts also recognised hsps. In vivo activated interleukin-2 (IL-2) receptor positive T cells were expanded from renal allografts, which had been removed because of irreversible rejection, by 1 week of culture in IL-2 only, followed by stimulation with OKT-3 in combination with IL-2. One T cell line was obtained from each graft. FACS analysis of the lines demonstrated that 51% and 53% of the cells were CD4+ and 45% and 42% CD8+; 4% and 5% were gamma delta + T cells. Both lines had a significant proliferative response when stimulated with recombinant human hsp 72. In contrast, hsp 65 did not induce stimulation. The lines also showed a proliferative response upon stimulation with epithelial kidney cells, purified and cultured from the respective rejected kidneys. These results demonstrate that T cells propagated from rejected human kidney allografts recognize hsp 72. This suggests that hsp-specific T cells may play a role in the rejection process of allograft kidneys.
Trieb
K
K
Department of Transplant Surgery, University Hospital Innsbruck, Austria.
Grubeck-Loebenstein
B
B
Eberl
T
T
Margreiter
R
R
D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016853:Muromonab-CD3 / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007376:Interleukin-2; D016853:Muromonab-CD3; D015375:Receptors, Interleukin-2