title: Immunocytochemical localization of glutamate-like immunoreactivity within the nervous system of the cestode Mesocestoides corti and the trematode Fasciola hepatica.
abstract: The localization and distribution of glutamate-like immunoreactivity (IR) in the nervous system of both the cestode Mesocestoides corti and the trematode Fasciola hepatica has been determined by an indirect immunofluorescent technique, in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was widespread in the central (CNS) and peripheral (PNS) nervous systems of both species examined. In the CNS, IR was evident in nerve cells and fibres in the cerebral ganglia, the cerebral commissure and the dorsal, ventral and longitudinal nerve cords. In the peripheral nervous system (PNS) of M. corti, IR was apparent in nerve plexuses associated with the subtegmental musculature and the musculature associated with the anteriorly positioned suckers. In F. hepatica, IR was evident in the innervation of both the oral and the ventral suckers. In the reproductive system of F. hepatica, glutamate-IR was observed around the ootype/Mehlis' gland complex.
authors: School of Biology and Biochemistry, Medical Biology Centre, Queen's University of Belfast, Northern Ireland. djab@soton.ac.uk/d.brownlee@queens-belfast.ac.uk
authors: D J
authors: DJ
authors: Brownlee
authors: I
authors: I
authors: Fairweather
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D005210:Fasciola hepatica / Q000378:metabolism* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D018698:Glutamic Acid / Q000378:metabolism*; D008645:Mesocestoides / Q000378:metabolism* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D017933:Peripheral Nervous System / Q000378:metabolism*
chemical_list: D018698:Glutamic Acid