Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli.
The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded-DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.
Rosche
W A
WA
Department of Biochemistry and Biophysics, Texas A&M University, Texas Medical Center, Houston 77030-3303, USA.
Jaworski
A
A
Kang
S
S
Kramer
S F
SF
Larson
J E
JE
Geidroc
D P
DP
Wells
R D
RD
Sinden
R R
RR
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009223:Myotonic Dystrophy / Q000235:genetics; D010957:Plasmids; D017384:Sequence Deletion; D018911:Trinucleotide Repeats
D004268:DNA-Binding Proteins