Programmed cell death during metamorphosis in the blow-fly Calliphora vomitoria.
During metamorphosis, the salivary glands of the blow-fly undergo programmed cell death. Data is presented indicating that this programmed cell death does not in many respects emulate classical apoptosis. The cells are seen to vacuolate and swell rather than condense and shrink. There appears to be a transient enhancement in autophagy and an increase in acid phosphatase activity. This is followed by the characteristic appearance of ribosomal and extracisternal sources of the enzyme leading to autolysis. There appears to be no lysosomal leakage of acid phosphatase. As in apoptosis, the mitochondria persist until the cell fragments. The nucleus, however, does not show the distinct chromatin margination and blebbing that is typical of apoptosis. These changes are compared with necrotic changes induced by experimental anoxia. Overall the results show that a programmed cell death distinct from classical apoptosis is taking place.
Bowen
I D
ID
School of Pure and Applied Biology, University of Wales College Cardiff, United Kingdom.
Mullarkey
K
K
Morgan
S M
SM
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004175:Diptera / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D006651:Histocytochemistry / Q000379:methods; D000860:Hypoxia / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008675:Metamorphosis, Biological / Q000502:physiology*; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009336:Necrosis; D012270:Ribosomes / Q000648:ultrastructure; D012469:Salivary Glands / Q000166:cytology / Q000502:physiology; D014617:Vacuoles / Q000201:enzymology / Q000648:ultrastructure
D000135:Acid Phosphatase