Neuroplastic changes in the hypothalamic arcuate nucleus: the estradiol effect is accompanied by increased exoendocytotic activity of neuronal membranes.
1. In the rat hypothalamic arcuate nucleus, estradiol induces coordinated changes in the number of axosomatic synapses, the amount of glial ensheathing, and the ultrastructure of the membrane of neuronal somas. In the present study we used conventional electron microscopy and freeze-fracture to examine cellular mechanisms responsible for the estradiol-induced changes at the membrane level. 2. In freeze-fracture replicas taken 10-60 min and 24 hr after injection of 17 beta-estradiol to adult ovariectomized females, it was found that there was a rapid increase in the number of exoendocytotic images that reached a plateau by 30 min. 3. In thin sections from animals injected 24 hr earlier we demonstrated a significant increase in coated vesicles in the periphery of the neurons and coated pits in the perikaryal membranes and decreased axosomatic synapses. 4. We conclude that these morphological alterations are signaling estrogen-induced transport and/or turnover of perikaryal membrane constituents and extracellular components which may affect interneuronal and neuroglial interactions.
Párducz
A
A
Institute of Biophysics, Biological Research Center, Szeged, Hungary.
Szilágyi
T
T
Hoyk
S
S
Naftolin
F
F
Garcia-Segura
L M
LM
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000502:physiology*; D001692:Biological Transport / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D003034:Coated Pits, Cell-Membrane / Q000502:physiology / Q000648:ultrastructure; D018699:Coated Vesicles / Q000502:physiology / Q000648:ultrastructure; D004705:Endocytosis / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005614:Freeze Fracturing; D008854:Microscopy, Electron / Q000379:methods; D018715:Microscopy, Video; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D009862:Online Systems; D051381:Rats; D017208:Rats, Wistar
D015815:Cell Adhesion Molecules; D004958:Estradiol