ITI (inter-alpha trypsin inhibitor) phenotyping by immunogold staining.
Polymorphism of Inter-alpha-Trypsin Inhibitor (ITI) was investigated in 685 unrelated Japanese individuals by isoelectric focusing followed by electroblotting and immunogold silver staining. The allele frequency estimates were ITI*1 = 0.455 +/- 0.013, ITI*2 = 0.516 +/- 0.013, ITI*3 = 0.028 +/- 0.0045 and ITI*4 = 0.001 +/- 0.001 and the phenotype frequencies fitted the Hardy-Weinberg equilibrium. The ITI system could be regarded as a potential genetic marker in paternity testing and anthropological studies.
Kimura
Y
Y
Department of Legal Medicine, Yamanashi Medical University, Japan.
Kido
A
A
Nishizono
T
T
Inoue
T
T
Oya
M
M
D000483:Alleles; D000510:Alpha-Globulins / Q000032:analysis / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D007150:Immunohistochemistry; D007564:Japan; D010641:Phenotype; D011110:Polymorphism, Genetic; D016622:Silver Staining; D014361:Trypsin Inhibitors / Q000097:blood / Q000235:genetics*
D000510:Alpha-Globulins; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor