Effect of 3'-untranslated region on expression of TNF alpha cDNA in Escherichia coli.
A construct pRL-rhTNF alpha 2, in which the 110 bp 3'-untranslated region of rhTNF alpha cDNA was deleted, was transformed in E.coli. The expression level of several positive transformants was determined. The results showed that the expression of pRL-TNF alpha 2 was stable and the biological characteristics of the protein product were the same as that of pRL-rhTNF alpha. Furthermore, the expression level of pRL-rhTNF alpha 2 was increased. It is suggested that the 3'-untranslated region may have some effects on gene expression. A TA-rich sequence, TTTATTA, contained in the 3'-untranslated region of pRL-rhTNF alpha may be involved in the inhibitory effect on gene expression.
Chang
J
J
Institute of Genetics, Fudan University, Shanghai, China.
Cai
W
W
Xu
L
L
Li
C
C
Zhao
S
S
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003603:Cytotoxins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000633:toxicity
D003603:Cytotoxins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha