title: Effect of desipramine on spontaneous activity of hippocampal CA1 neuron after transient cerebral ischemia in rats.
abstract: OBJECTIVE
  To study the spontaneous firing of CA1 neurons in rat hippocampus after transient cerebral ischemia and the effect of desipramine (Des) on the post-ischemic electric activity of CA1 neurons.
  
  
  METHODS
  Single-unit extracellular recordings were performed in rats on d 3 after 10 min of cerebral ischemia by occlusion of 4 arteries. Des and saline were injected into a tail vein. The histological changes of CA1 neurons was assessed by the neuronal density of the CA1 sector.
  
  
  RESULTS
  The spontaneous firing rate of CA1 neurons on d 3 after ischemia was enhanced in comparison with the control value. Des (0.2 and 0.4 mg.kg-1, i.v., n = 5 & 6, respectively) reduced dose-dependently the increase of firing rate with maximal inhibition by 6 min (58% & 85%) to 9 min (69% & 94%) (vs vehicle group, P < 0.01). About 50% cells in CA1 region showed necrotic changes.
  
  
  CONCLUSIONS
  Des antagonized the hyperexcitability of CA1 neurons after cerebral ischemia.
authors: Department of Pharmacology, Nanjing Railway Medical College, China.
authors: Z T
authors: ZT
authors: Zhu
authors: X X
authors: XX
authors: Zhang
authors: J
authors: J
authors: Liu
authors: G Z
authors: GZ
authors: Jin
mesh_terms: D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology*; D003891:Desipramine / Q000494:pharmacology*; D004594:Electrophysiology; D006624:Hippocampus / Q000502:physiology*; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D008297:Male; D009474:Neurons / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000929:Antidepressive Agents, Tricyclic; D003891:Desipramine