The flying cylinder: a new algorithm for filament recognition in noisy stereo images.
We present a new method of automatic 3D filament representation which uses stereo micrographs to reconstruct three-dimensional trajectories of filament-like objects as DNA molecules. The method deals with low contrasted and noisy images, as obtained from cryovitrified samples by means of electron microscopy. The three-dimensional information is extracted from skeletizing simultaneously both images of a given stereo-pair, instead of processing them separately. The main advantages of the technique are reproducibility and speed, compared to the reconstruction done by manual registration, i.e., clicking on the stereo micrographs.
Piñol I Margalef
F
F
Signal Processing Laboratory, Swiss Federal Institute of Technology, Lausanne, Switzerland. pinol/lts.epfl.ch
Furrer
P
P
Kunt
M
M
Dubochet
J
J
D003196:Computer Graphics; D004247:DNA / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008961:Models, Structural; D015203:Reproducibility of Results; D012984:Software
D004247:DNA