Culture and IgE synthesis of nasal B cells.
The interplay of inflammatory cells, mediators and cytokines during type I allergic reactions in the nose is well described. But even though allergen-specific IgE is known to play a central role for the induction of these events, little is known about nasal B cells and their role in the local allergic reaction. It was the aim of the present study to examine the possibility to isolate and culture B cells from the nose. For this purpose allergic and nonallergic volunteers were challenged by nasal provocation with allergen. Cells were collected sequentially after nasal provocation by nasal lavage, B cells grown in the CD40 system and IgE production assessed by enzyme-linked filter spot assay and radioimmunoassay. IgE-producing B cells were detected after culture of nasal lavage cells in the CD40 system. Elevated levels of IgE were measured in culture supernatants from cells collected during the late-phase allergic reaction. We conclude that B cells can be isolated from the nose and may serve as an interesting source of B cells after in vivo contact with antigen.
Zürcher
A W
AW
Institute of Immunology and Allergology, Inselspital, Bern, Switzerland.
Derer
T
T
Lang
A B
AB
Stadler
B M
BM
D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology; D002478:Cells, Cultured; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D009296:Nasal Cavity / Q000737:chemistry / Q000276:immunology*; D017817:Nasal Lavage Fluid / Q000166:cytology; D009299:Nasal Provocation Tests; D010936:Plant Extracts / Q000276:immunology; D006109:Poaceae / Q000276:immunology
D019013:CD40 Antigens; D010936:Plant Extracts; D015847:Interleukin-4; D007073:Immunoglobulin E