Interleukin 2 is a lectin that associates its receptor with the T-cell receptor complex.
To determine the nature of the mechanism by which the binding of interleukin-2 (IL-2) to its receptor (IL-2R beta) induces IL-2R beta phosphorylation by the tyrosine kinase p56lck associated with the T-cell receptor (TCR) complex, we investigated the possibility that this mechanism was due to the putative lectin activity of IL-2 ([Sherblom, Sathyamoorthy, Decker and Muchmore (1989) J. Immunol. 143, 939-944]. Here we demonstrate that IL-2 is a calcium-independent lectin specific for oligomannosidic N-glycans with five and six mannose residues. This lectin activity is preserved after binding of IL-2 to IL-2R beta. IL-2 behaves as a bifunctional molecule that associates IL-2R beta with specific glycoprotein ligands of the TCR complex including a glycosylated form of CD3.
Zanetta
J P
JP
Centre de Neurochimie du CNRS, Strasbourg, France.
Alonso
C
C
Michalski
J C
JC
D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology; D010766:Phosphorylation; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D019061:src-Family Kinases / Q000378:metabolism
D006023:Glycoproteins; D007376:Interleukin-2; D037102:Lectins; D009844:Oligosaccharides; D011134:Polysaccharides; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; C059525:cerebellar soluble lectin; C028569:oligomannoside; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019061:src-Family Kinases; D012260:Ribonucleases; C029600:ribonuclease B