Fluorometric titration of the mitochondrial ADP/ATP carrier protein in muscle homogenate with atractyloside derivatives.
We describe here the chemical synthesis of the novel methylanthraniloyl (Mant-) derivative of atractyloside (ATR), which is a specific inhibitor of the mitochondrial ADP/ATP carrier. The spectral properties of Mant-ATR and naphthoyl-ATR (N-ATR) are analyzed. Both derivatives bind to the membrane-bound ADP/ATP carrier at the same sites as ATR and carboxyatractyloside (CATR). When Mant-ATR and N-ATR are displaced by CATR, their fluorescence emissions are decreased and increased, respectively. These fluorescence changes allow the titration of the CATR binding sites and therefore the quantitation of the amount of ADP/ATP carrier protein in a biological preparation. The validity of the fluorometric titration was tested with beef heart mitochondria and confirmed by binding assays using radioactive ATR. The fluorometric method was applied to rabbit skeletal muscle homogenate and the results of titration were confirmed by binding assays of radioactive ATR. The reliability of the fluorometric method was assessed by comparing the amounts of CATR binding sites and the content of heme aa3 in muscle homogenates and in isolated mitochondria from the same homogenates. Because of its high sensitivity, the fluorometric titration of the ADP/ATP carrier requires small amounts of tissue. Mant-ATR and N-ATR can therefore be considered as convenient, reliable, and sensitive probes to quantify the amount of ADP/ATP carrier and detect a putative carrier protein deficiency in biopsy samples from human patients suffering from myopathies with no clear identified etiology.
Roux
P
P
Laboratoire de Biochimie (URA 1130 CNRS), Grenoble, France.
Le Saux
A
A
Fiore
C
C
Schwimmer
C
C
Dianoux
A C
AC
Trézéguet
V
V
Vignais
P V
PV
Lauquin
G J
GJ
Brandolin
G
G
D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives*; D001665:Binding Sites; D002417:Cattle; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000032:analysis* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods; D062367:ortho-Aminobenzoates
D062367:ortho-Aminobenzoates; D001278:Atractyloside; D000226:Mitochondrial ADP, ATP Translocases