Genomic structure, chromosome location, and alternative splicing of the human NKG2A gene.
A cosmid containing the human NKG2A gene was isolated. The gene was partially sequenced, revealing 7 exons, including one 5' untranslated exon. The 54 base pair exon 5 was missing in the published cDNA clone NKG2B, consistent with the existence of differential splicing. This was confirmed by reverse transcription-polymerase chain reaction of total RNA from normal lymphocytes. The NKG2A gene was mapped by FISH to chromosome 12p12. 3-p13.1 in proximity to the CD69 and Prp genes. These data support the presence of a human lectin-like NK gene complex, analogous to the NK complex on mouse chromosome 6.
Plougastel
B
B
Human Immunogenetics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, England.
Jones
T
T
Trowsdale
J
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002881:Chromosomes, Human, Pair 12; D003360:Cosmids; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007694:Killer Cells, Natural; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary