title: Crystallization of the A alpha subunit of protein phosphatase 2A.
abstract: The A alpha subunit of human protein phosphatase 2A forms crystals in space group P2(1) with cell dimensions a = 104.0, b = 174.9, c = 168.2 A, and beta angle = 90.2 degrees. At cryogenic temperatures, the crystals diffracted to a resolution limit of approximately 3.0 A. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 A and merged to provide a set that is 93% complete, with Rsym of 9.9%.
authors: Department of Pathology, University of California at San Diego, La Jolla 92093-0612, USA.
authors: M O
authors: MO
authors: Skidmore
authors: M R
authors: MR
authors: Sawaya
authors: S
authors: S
authors: Parkin
authors: B
authors: B
authors: Rupp
authors: H
authors: H
authors: Hope
authors: S J
authors: SJ
authors: Everse
authors: G
authors: G
authors: Walter
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005615:Freezing; D006801:Humans; D010749:Phosphoprotein Phosphatases / Q000737:chemistry*; D054648:Protein Phosphatase 2
chemical_list: D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2