Novel DNase I hypersensitive sites in the 3'-flanking region of the human c-myc gene.
DNase I hypersensitivity regions correlate with genetic regulatory loci and binding sites for sequence-specific DNA-binding proteins. We present data supporting the presence of novel DNase 1 hypersensitive sites (which we have designated sites VI-IX) in both the body of the human c-myc gene downstream from exon 2 and the 3'-flanking region of the c-myc gene in HL-60 cells. All of these novel DH sites are markedly decreased when HL-60 cells are treated with either dimethyl sulfoxide (DMSO) or retinoic acid. Moreover, a similar pattern of DNase I hypersensitive sites in this region of c-myc was present in MCF-7 human breast cancer cells growing in culture. Our results suggest a potential role for these sites in transcriptional regulation of the human c-myc gene.
Murphy
L C
LC
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Huzel
N
N
Davie
J R
JR
D001483:Base Sequence; D001943:Breast Neoplasms; D004247:DNA / Q000187:drug effects; D004268:DNA-Binding Proteins; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology
D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D014212:Tretinoin; D004247:DNA; D003850:Deoxyribonuclease I; D004121:Dimethyl Sulfoxide