title: Interference of reovirus strains occurs between the stages of uncoating and dsRNA accumulation.
abstract: Interference of wild-type reovirus growth by some temperature-sensitive (ts) mutant viruses under non-permissive conditions or by other wild-type isolates has been demonstrated; however, the stage of the virus replication cycle at which interference occurs has not been defined. Examination of the time-course of the yields of T1 Lang (T1L) dsRNA in the progeny of mixed infections of T1L with T3 Dearing (T3D) or with a panel of T3D ts mutants at a non-permissive temperature revealed that interference takes place by 8-10 h post-infection and occurs prior to or at the same time as accumulation of reovirus dsRNA. Taken together with our previous results, these data indicate that interference occurs during a window between virus uncoating and synthesis of dsRNA in the reovirus replication cycle, probably at the stage of assembly of primary reovirus particles.
authors: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors: M N
authors: MN
authors: Rozinov
authors: B N
authors: BN
authors: Fields
mesh_terms: D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D012089:Mammalian orthoreovirus 3 / Q000235:genetics / Q000502:physiology*; D018165:Orthoreovirus / Q000235:genetics / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D016601:RNA-Binding Proteins; D013997:Time Factors; D014762:Viral Interference; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins; C055356:mu1 protein, Reovirus; C053179:sigma protein 3, Reovirus