Studies of Qingyangshen (II): modulatory effect of co-treatment with qingyangshen and diphenylhydantoin sodium on rat hippocampal c-fos expression during seizures.
We have reported that, after KA treatment in rats, there was first a significant increase in hippocampal c-fos expression during acute seizures and then a long-term inhibition in hippocampal c-fos expression during chronic seizures. In this experiment, we examined the modulatory effect of Qingyangshen (QYS), a traditional Chinese medicine with antiepileptic property, and diphenylhydantoin sodium (DPH) on hippocampal c-fos expression during seizures. We found that after intraperitoneal injection of QYS + DPH (15 mg/kg and 50 mg/kg respectively, q.o.d. x 6), the peak level of hippocampal c-fos mRNA induction during KA-induced acute seizures was reduced by 56.27%; while the inhibition of hippocampal c-fos expression during KA-induced chronic seizures was disinhibited to control level. It is suggested that the inhibitory effect of QYS + DPH on KA-induced seizures, chronic seizures in particular, may have much to do with its modulatory effect on hippocampal c-fos expression.
Guo
Q
Q
Department of Neurology, Chinese PLA General Hospital, PLA Postgraduate Medical School, Beijing, P.R. China.
Kuang
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P
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid; D008297:Male; D010672:Phenytoin / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000235:genetics*
D000927:Anticonvulsants; D004365:Drugs, Chinese Herbal; D016760:Proto-Oncogene Proteins c-fos; D010672:Phenytoin; D007608:Kainic Acid