Hepatocyte death following transforming growth factor-beta 1 addition.
Apoptosis is a morphological term which describes a sequence of events finally leading to cell death. In epithelial organs, induction of cell death is closely linked to an inhibitor of epithelial growth, transforming growth factor-beta 1 (TGF-beta 1). In this paper, we describe the morphology of TGF-beta 1-induced apoptosis in hepatocytes of the hyperplastic liver and primary cultures. Chromatin condensation, a hallmark of apoptosis, was observed in primary hepatocytes by confocal and vital UV microscopy. In addition, we have applied the morphological detection of DNA strand breaks both by in situ tailing (ISTAIL) and in situ nick translation (ISNT).
Oberhammer
F
F
MRC Toxicology Unit, University of Leicester, United Kingdom.
Froschl
G
G
Tiefenbacher
R
R
Inayat-Hussain
S H
SH
Cain
K
K
Stopper
H
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D004247:DNA / Q000032:analysis / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D008099:Liver / Q000737:chemistry / Q000473:pathology* / Q000502:physiology*; D018613:Microscopy, Confocal; D008860:Microscopy, Ultraviolet; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D002843:Chromatin; D016212:Transforming Growth Factor beta; D004247:DNA