Acetylcholinesterase and choline uptake in striatum from rats with varying sleeping times.
Male rats were injected with ethanol as a 25% solution (3.5 g/kg body weight) and then the duration of the retention of the righting reflex (DRR) as well as the duration of ethanol-induced sleep (DEIS) were recorded. On the basis of DEIS in 60 rats, we selected four groups of six rats each: short-sleep group (SS, 34 +/- 5 min), intermediate-sleep (IS, 118 +/- 3 min), long-sleep (LS, 186 +/- 5 min) and non-sleep rats (NS, 0 min). The striatal crude mitochondrial fraction was assayed for acetylcholinesterase activity (AChE, EC 3.1.1.7) and high-affinity choline uptake. NS rats manifested similarity of the above parameters to those determined in SS and IS rats. LS rats were distinguished by a higher AChE activity in comparison with NS animals.
Chumakova
O V
OV
Institute of Biochemistry, Academy of Sciences of Belarus.
Liopo
A V
AV
D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism*; D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000818:Animals; D002794:Choline / Q000378:metabolism*; D003342:Corpus Striatum / Q000201:enzymology*; D046508:Culture Techniques; D007274:Injections, Intraperitoneal; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D004856:Postural Balance / Q000502:physiology; D051381:Rats; D012894:Sleep Stages / Q000502:physiology*
D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine