Inhibitory effects of 1-hydroxyethylidene-1,1-bisphosphonate and Chinese traditional (kampo) medicines on calcification of the heart and tongue in DBA/2NCrj mice.
The effects of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) and two Chinese traditional (Kampo) medicines, Dai-saiko-to and Saiko-ka-ryukotsu-borei-to on spontaneous soft tissue (heart and tongue) mineralization in DBA/2NCrj mice were studied. These agents were given orally for 4 weeks to DBA/2NCrj mice. After 2 weeks of administration in the heart, 0.006 and 0.03% (w/v) HEBP decreased calcium content by 90 and 30%, respectively, while 0.27 and 2.7 mg/ml Dai-saiko-to reduced calcium content by 30 and 45%, respectively. Saiko-ka-ryukotsu-borei-to (0.27 mg/ml) reduced both calcium and phosphorus content by 50 and 35%, respectively. However, their inhibitory effects on the heart were not observed after 4 weeks of administration. The compounds delayed the onset of increases of bulk calcium and phosphorus content. In the tongue, at 4 weeks, 0.006 and 0.03% (w/v) HEBP reduced calcium content by 30 and 45%, respectively, while two Kampo medicines (at both concentrations used) significantly reduced the content of calcium (by 27-79%) and phosphorus (by 24-32%). These results strongly suggest that two Kampo medicines as well as HEBP may be useful in preventing and curing soft tissue calcification.
Hidaka
S
S
Department of Oral Biochemistry, Fukuoka Dental College, Japan.
Okamoto
Y
Y
Abe
K
K
Miyazaki
K
K
Suekawa
M
M
Liu
S Y
SY
D000818:Animals; D002114:Calcinosis / Q000188:drug therapy*; D004164:Diphosphonates / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008811:Mice, Inbred DBA; D013997:Time Factors; D014059:Tongue / Q000187:drug effects*
D004164:Diphosphonates