[Accumulation sites of kanamycin in cochlear basal membrane cells].
The dissection technique of cochlear basal membrane, the fractional technique of tissular homogenization, and the radiometry technique were used to study the toxicity of kanamycin on the hair cells. It was found that after the cochlea was perfused with a 0.25% perilymph solution of kanamycin, there were 3.14 fg KM in a nucleus, 7.61 fg KM in a mitochondria and 21.16 fg KM in a lysosome. It showed that after entering the cell, KM mainly accumulated with in the mitochondria. Simultaneously the lysosome also phagocytized a lot of kanamycin. The germicidal mechanism of kanamycin is to inhibit the synthesis of protein in bacteria and the mitochondria has the same way of protein synthesis, the mitochondria can combine with kanamycin specifically; the drug in lysosome may be related to its phagocytic function.
Ding
D
D
Renji Hospital, Shanghai Medical University.
Jin
X
X
Zhao
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001485:Basement Membrane / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism*; D006168:Guinea Pigs; D007612:Kanamycin / Q000493:pharmacokinetics*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism
D000900:Anti-Bacterial Agents; D007612:Kanamycin