title: Activation of human platelets by lysophosphatidic acid.
abstract: The biochemical mechanisms of platelet activation by lysophosphatidic acid were investigated. Lysophosphatidic acid interacts with a membrane receptor coupled to the inhibitory GTP-binding protein Gi and produces a rapid decrease of the intracellular concentration of cAMP. Aggregation of gel-filtered platelets by lysophosphatidic acid requires the presence of extracellular CaCl2, as this phospholipid does not induce secretion of platelet dense granules. Platelet activation by lysophosphatidic acid in the absence of extracellular CaCl2 does not involve phospholipase C activation, as evaluated by measuring mobilization of Ca2+ from internal stores and pleckstrin phosphorylation, but causes the rapid tyrosine phosphorylation of several intracellular proteins. Our results indicate that activation of intracellular tyrosine kinases is not secondary to Ca2+ mobilization and protein kinase C activation in lysophosphatidic acid-stimulated platelets.
authors: Department of Biochemistry, University of Pavia, Italy.
authors: M
authors: M
authors: Torti
authors: E
authors: E
authors: Tolnai Festetics
authors: A
authors: A
authors: Bertoni
authors: F
authors: F
authors: Sinigaglia
authors: C
authors: C
authors: Balduini
mesh_terms: D000345:Affinity Labels; D001386:Azides / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008246:Lysophospholipids / Q000494:pharmacology*; D015539:Platelet Activation / Q000187:drug effects*; D010979:Platelet Function Tests
chemical_list: D000345:Affinity Labels; D001386:Azides; D008246:Lysophospholipids; C031907:GTP gamma-4-azidoanilide; D006160:Guanosine Triphosphate; D000242:Cyclic AMP