title: [Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].
abstract: OBJECTIVE
  To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.
  
  
  METHODS
  The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.
  
  
  RESULTS
  Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.
  
  
  CONCLUSIONS
  In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.
authors: Fourth Military Medical University, Xi'-an.
authors: Q
authors: Q
authors: Wang
authors: Z
authors: Z
authors: Chen
authors: W
authors: W
authors: Wang
mesh_terms: D000236:Adenoma / Q000473:pathology; D018033:Antibodies, Bispecific / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008113:Liver Neoplasms / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology*; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments