Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to 8q22 by in situ hybridization.
We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization. The additional peak obtained with the former method is attributed to the higher sensitivity of this technique, which results in hybridization of the probe to the less conserved pseudogene. We therefore conclude that the functional gene is most likely located at 8q22.
Malaney
S
S
Department of Genetics, Research Institute, Toronto. Ont., Canada.
Heng
H H
HH
Tsui
L C
LC
Shi
X M
XM
Robinson
B H
BH
D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D014450:Electron Transport Complex III / Q000235:genetics*; D005796:Genes; D006801:Humans; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D014450:Electron Transport Complex III