Efferent projections of the retrorubral nucleus to the substantia nigra and ventral tegmental area in cats as shown by anterograde tracing.
The aim of the present study was to determine whether the retrorubral nucleus projects to the dopaminergic nuclei in the ventral midbrain of the cat. For this purpose, injections of biotinylated dextran-amine or Phaseolus vulgaris-leucoagglutinin were placed into the retrorubral nucleus under stereotaxic guidance. The tracers were visualized by means of (immuno) histochemical procedures. In addition, tyrosine hydroxylase immunohistochemistry was used to evaluate the location of the injection sites and the distribution of the anterogradely labeled fibers. Both tracers reveal the same topography of labeled fibers in the ventral mesencephalon. Labeled fibers with varicosities were found ipsilaterally in the substantia nigra pars compacta, the substantia nigra pars lateralis, the ventral tegmental area and, contralaterally, in the substantia nigra pars compacta, the ventral tegmental area, and the retrorubral nucleus. A considerable number of labeled axons with varicosities were observed to be wrapped around the dendrites and perikarya of tyrosine hydroxylase-positive neurons in these areas. The present results are discussed in view of the possible role of the A8 dopaminergic cell group in the coordination of A9 nigrostriatal and A10 mesolimbic systems, as well as in the progressive pathology seen in patients suffering from Parkinson's disease.
Arts
M P
MP
Department of Psychoneuropharmacology, Graduate School of Pathophysiology of the Nervous System, University of Nijmegen, The Netherlands.
Groenewegen
H J
HJ
Veening
J G
JG
Cools
A R
AR
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D002415:Cats; D003911:Dextrans; D004298:Dopamine / Q000502:physiology*; D004525:Efferent Pathways / Q000502:physiology; D005456:Fluorescent Dyes; D007267:Injections; D008297:Male; D009474:Neurons / Q000201:enzymology*; D010835:Phytohemagglutinins; D012012:Red Nucleus / Q000166:cytology / Q000502:physiology*; D013378:Substantia Nigra / Q000166:cytology / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D017557:Ventral Tegmental Area / Q000166:cytology / Q000502:physiology*
D003911:Dextrans; D005456:Fluorescent Dyes; D010835:Phytohemagglutinins; C076397:biotinylated dextran amine; C100156:leukoagglutinins, plants; D001710:Biotin; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine