Molecular cloning and expression of alpha parvalbumin in the guinea pig cochlea.
We have cloned and sequenced an alpha parvalbumin cDNA from the guinea pig cochlea. The deduced amino acid sequence shows greater identity with the rabbit sequence (86.3%) than with other mammalian sequences (< 82%). Using in situ hybridization and immunohistochemistry, alpha parvalbumin mRNA and protein were found in primary auditory neurons and inner hair cells, in agreement with RT-PCR data showing alpha parvalbumin mRNA expression in the spiral ganglion and the organ or Corti.
Soto-Prior
A
A
Neurobiologie de l'Audition-Plasticité Synaptique, INSERM U 254, Université de Montpellier.
Cluzel
M
M
Renard
N
N
Ripoll
C
C
Lavigne-Rebillard
M
M
Eybalin
M
M
Hamel
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003051:Cochlea / Q000378:metabolism*; D018076:DNA, Complementary / Q000302:isolation & purification*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
D018076:DNA, Complementary; D010320:Parvalbumins