Covalent enzyme immobilization on paramagnetic polyacrolein beads.
An optimized procedure of covalent glucose oxidase, urease, Bacillus subtilis alpha-amylase and Bacillus licheniformis alpha-amylase immobilization on paramagnetic, non-porous, polyacrolein beads is presented. The resulting insolubilized enzymes can be employed for extended periods of time without loss of activity. The conditions were optimized for maximizing the activity of the linked enzyme. Coated beads bearing up to 15 micrograms active enzyme/mg(beads) were obtained on reproducible basis. The paramagnetic feature of the particles facilitates the enzyme handling. In the magnetic field, the enzyme separation is fast and complete. Thus, the paramagnetic beads represent an excellent carrier for immobilized enzymes.
Varlan
A R
AR
Faculty of Electronics, Katholieke Universiteit Leuven, Heverlee, Belgium.
Sansen
W
W
Van Loey
A
A
Hendrickx
M
M
D000171:Acrolein; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008567:Membranes, Artificial; D008839:Microelectrodes; D008863:Microspheres; D011108:Polymers; D012996:Solutions; D013696:Temperature; D013997:Time Factors
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011108:Polymers; D012996:Solutions; C029975:polyacrolein; D000431:Ethanol; D000171:Acrolein