title: Specific DNA replication mutations affect telomere length in Saccharomyces cerevisiae.
abstract: To investigate the relationship between the DNA replication apparatus and the control of telomere length, we examined the effects of several DNA replication mutations on telomere length in Saccharomyces cerevisiae. We report that a mutation in the structural gene for the large subunit of DNA replication factor C (cdc44/rfc1) causes striking increases in telomere length. A similar effect is seen with mutations in only one other DNA replication gene: the structural gene for DNA polymerase alpha (cdc17/pol1) (M.J. Carson and L. Hartwell, Cell 42:249-257, 1985). For both genes, the telomere elongation phenotype is allele specific and appears to correlate with the penetrance of the mutations. Furthermore, fluorescence-activated cell sorter analysis reveals that those alleles that cause elongation also exhibit a slowing of DNA replication. To determine whether elongation is mediated by telomerase or by slippage of the DNA polymerase, we created cdc17-1 mutants carrying deletions of the gene encoding the RNA component of telomerase (TLC1). cdc17-1 strains that would normally undergo telomere elongation failed to do so in the absence of telomerase activity. This result implies that telomere elongation in cdc17-1 mutants is mediated by the action of telomerase. Since DNA replication involves transfer of the nascent strand from polymerase alpha to replication factor C (T. Tsurimoto and B. Stillman, J. Biol. Chem. 266:1950-1960, 1991; T. Tsurimoto and B. Stillman, J. Biol. Chem. 266:1961-1968, 1991; S. Waga and B. Stillman, Nature [London] 369:207-212, 1994), one possibility is that this step affects the regulation of telomere length.
authors: Department of Pharmacology, Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093-0651, USA.
authors: A K
authors: AK
authors: Adams
authors: C
authors: C
authors: Holm
mesh_terms: D000483:Alleles; D018797:Cell Cycle Proteins / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000648:ultrastructure*; D004257:DNA Polymerase II / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics*; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005800:Genes, Fungal; D009154:Mutation; D051818:Replication Protein C; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D019098:Telomerase / Q000502:physiology; D016615:Telomere / Q000648:ultrastructure*
chemical_list: D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; C084903:RFC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D019098:Telomerase; D004257:DNA Polymerase II; D051818:Replication Protein C