Trapping of 134CS+ in frog skin epithelium as a function of short circuit current.
In the sodium/potassium pump of the isolated frog skin epithelium, 134CS+ can to a certain degree replace potassium. If 134CS+ in almost a carrier-free amount is added to the solution bathing the inside of the frog skin in an Ussing chamber, there will be an uptake of 134 CS+ into the epithelium cells which is proportional to the short circuit current. Transepithelial flux of 134CS+ in leaky epithelia, for instance small intestine and frog skin glands, indicates a paracellular pathway. The consequences of these findings for the recycling-theory are discussed.
Ussing
H H
HH
University of Copenhagen, Biochemistry Department, August Krogh Institut, Denmark.
Lind
F
F
D000818:Animals; D002588:Cesium Radioisotopes / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology; D008564:Membrane Potentials / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D011896:Rana temporaria / Q000502:physiology*; D012867:Skin / Q000166:cytology; D012879:Skin Physiological Phenomena; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000502:physiology
D002588:Cesium Radioisotopes; D004791:Enzyme Inhibitors; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase