Rapid in vitro test for determination of anti-amoebic activity.
A rapid microplate assay for the detection of potential compounds active against Entamoeba histolytica is described. The assay is based on the metabolic reduction of a tetrazolium salt by E. histolytica trophozoites as an indicator of their viability which may be measured photometrically. The method was validated by determining the dose-response characteristics of standard amoebicides and correlating optical density and cell number; it provides a convenient means of selecting potentially novel anti-amoebic compounds for subsequent testing in vivo.
Mukhopadhyay
R M
RM
Organon Research Centre, Calcutta, India.
Chaudhuri
S K
SK
D000563:Amebicides / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D013997:Time Factors
D000563:Amebicides; D007202:Indicators and Reagents; D009580:Nitroblue Tetrazolium