[CD45 gating of acute leukemia].
Flow cytometry is a powerful tool for immunophenotyping of acute leukemia. Gating of leukemic cells is currently performed on the two dimensional display of forward scatter (FSC) and side scatter (SSC). However, this gating method can not discriminate leukemic cells from contaminated normal cells. Therefore, we used a CD45 monoclonal antibody to detect leukemic cells (CD45dlm cells) in combination with the SSC parameter. This CD45-SSC gating method was very useful for immunotyping of AML and ALL, especially leukemia with a low percentage of blasts. We recommend this new gating method for more accurate immunophenotyping of acute leukemia.
Nishikawa
K
K
2nd Department of Internal Medicine, Nara Medical University, Kashihara.
Miyasaki
T
T
Tsukaguchi
N
N
Noma
Y
Y
Nakagawa
K
K
Narita
N
N
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007938:Leukemia / Q000175:diagnosis*; D017493:Leukocyte Common Antigens; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D017493:Leukocyte Common Antigens