A distinctive effect of CCCP on the transfer of erythromycin to 1-octanol: a possible model in promoting the intracellular antibiotic-accumulation through lipid in a staphylococcal cytoplasmic membrane.
Movement of erythromycin (EM), a basic antibiotic solute (pKa 8.6), in an aqueous buffer (pH7.6) at 37 degrees C into 1-octanol was found approximately twofold greater in the presence of another acidic and lipophilic solute, carbonylcyanide m-chlorophenylhydrazone (CCCP), when compared with the EM-movement in the absence of CCCP. CCCP appears to behave just like a carrier of EM, besides being a well-known inhibitor, such as the uncoupling agent of oxidative phosphorylation. The probability is discussed that such an analogous event takes place in the lipid layers of a bacterial-cell membrane.
Matsuoka
M
M
Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Nakajima
Y
Y
D020003:1-Octanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000737:chemistry*; D002462:Cell Membrane / Q000187:drug effects; D004917:Erythromycin / Q000737:chemistry / Q000378:metabolism*; D000442:Octanols / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000442:Octanols; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D004917:Erythromycin; D020003:1-Octanol