Comparative evaluation of agar gel precipitation, counterimmunoelectrophoresis and passive haemagglutination tests for the diagnosis of Dicrocoelium dendriticum infection in sheep and goats.
The sensitivity and specificity of the agar gel precipitation test (AGPT), counter immunoelectrophoresis (CIEP) and passive haemagglutination test (PHT) were evaluated for the diagnosis of Dicrocoelium dendriticum infection in naturally infected sheep and goats. Two hundred and forty five sheep and goat sera samples were tested using phosphate buffered saline, pH 7.2 extracted adult fluke antigen. CIEP detected 69.8% of the infected animals and was found to be the most sensitive, followed by PHT which detected 50.0% of the infected sheep and goats. AGPT was found to be the least sensitive, detecting only 23.8% of the infected animals. The specificity of
Jithendran
K P
KP
Disease Investigation Laboratory, Indian Veterinary Research Institute, Palampur, India.
Vaid
J
J
Krishna
L
L
D000003:Abattoirs; D000362:Agar; D000818:Animals; D001652:Bile Ducts / Q000469:parasitology; D011232:Chemical Precipitation; D003378:Counterimmunoelectrophoresis / Q000379:methods / Q000662:veterinary; D004011:Dicrocoeliasis / Q000175:diagnosis / Q000662:veterinary*; D004013:Dicrocoelium / Q000302:isolation & purification*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005782:Gels; D015511:Goat Diseases; D006041:Goats; D006386:Hemagglutination Tests / Q000379:methods / Q000662:veterinary; D008099:Liver / Q000382:microbiology; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases
D005782:Gels; D000362:Agar