Insulin-like growth factor-I attenuates delayed graft function in a canine renal autotransplantation model.
BACKGROUND
Insulin-like growth factor-I (IGF-I) has been shown to accelerate recovery in animal models of ischemic or toxic acute renal injury. Ischemic renal injury is frequently encountered after cadaveric transplantation manifested as delayed graft function. This study was performed to determine whether perfusion of kidneys with preservation solution supplemented with IGF-I would improve the course of renal injury in a canine autotransplantation model of delayed graft function.


METHODS
Dogs underwent unilateral nephrectomy with kidneys perfused and stored in Euro-Collins solution supplemented with vehicle (n = 11) or IGF-I (n = 8). After 24 hours of kidney preservation, a contralateral nephrectomy was performed and the stored kidney was autotransplanted. Renal function was examined for 5 days after the transplantation, and an inulin clearance was obtained at the time of death.


RESULTS
Compared with dogs that received kidneys preserved in the vehicle, dogs receiving the IGF-I preserved kidneys had significantly lower daily serum creatinine and blood urea nitrogen levels during the course of 5 days after transplantation. Inulin clearance at death was nearly double in the IGF-I treated animals compared with the vehicle-treated controls (1.37 +/- 0.16 ml/min/kg versus 0.77 +/- 0.13 ml/min/kg; p < 0.05).


CONCLUSIONS
Perfusion and storage of kidneys with preservation solution supplemented with IGF-I can attenuate the course of delayed graft function in a canine renal autotransplantation model. IGF-I may have potential for use in cadaveric human renal transplantation.
Petrinec
D
D
Washington University School of Medicine, St. Louis, MO 63110, USA.
Reilly
J M
JM
Sicard
G A
GA
Lowell
J A
JA
Howard
T K
TK
Martin
D R
DR
Brennan
D C
DC
Miller
S B
SB
D000818:Animals; D001806:Blood Urea Nitrogen; D003080:Cold Temperature; D003404:Creatinine / Q000097:blood; D004285:Dogs; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D016030:Kidney Transplantation; D009392:Nephrectomy; D014021:Tissue Preservation; D014182:Transplantation, Autologous
D007334:Insulin-Like Growth Factor I; D003404:Creatinine