[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].
OBJECTIVE
To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.


METHODS
The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.


RESULTS
Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.


CONCLUSIONS
In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.
Wang
Q
Q
Fourth Military Medical University, Xi'-an.
Chen
Z
Z
Wang
W
W
D000236:Adenoma / Q000473:pathology; D018033:Antibodies, Bispecific / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008113:Liver Neoplasms / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology*; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments