title: Ag, a novel protein secreted from Aplysia glia.
abstract: We have isolated a cDNA (ag for Aplysia glial) corresponding to an mRNA specific to the nervous system of Aplysia californica. In this study, we characterized the ag cDNA sequence and the distribution of ag mRNA and protein in the Aplysia nervous system. The ag cDNA contains an open reading frame that encodes a novel 29 kD protein. In situ hybridizations demonstrate that ag mRNA is conspicuously absent from the cell bodies of the large neurons constituting the external layer of the ganglia. Instead, it is largely confined to a subset of small, apparently non-neuronal cells surrounding the neurons at the border of the neuropil, is sparsely scattered within the neuropil, and is widespread within the connective nerves, a pattern consistent with glial localization. Polyclonal anti-ag antiserum recognizes a protein between 27 and 29 kD that is more broadly distributed, especially within the neuropil. The distributions of ag mRNA and protein, together with the presence of a putative signal peptide, suggest that ag protein is secreted. Two findings support this hypothesis: first, ag protein is detectable by western blot in Aplysia hemolymph. Second, full length ag protein expressed in COS cells is secreted, but ag lacking the putative signal peptide is not. Secretion from glia raises the possibility that this abundant protein may affect neighboring neurons.
authors: Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA.
authors: S T
authors: ST
authors: Lockhart
authors: I B
authors: IB
authors: Levitan
authors: C W
authors: CW
authors: Pikielny
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: C098941:Ag protein, Aplysia californica; D018076:DNA, Complementary; D009479:Neuropeptides; D012333:RNA, Messenger