Surface plasmon resonance studies of immunoreactions utilizing disposable diffraction gratings.
The specificity of protein binding between immuno-gamma globulin (IgG) and anti-IgG has been investigated by means of surface plasmon resonance measurements, with a view to determining whether a perspex replica of a holographic diffraction grating can be used efficiently as the momentum-coupling device. The replicas are easy to fabricate, of low cost, and may be useful as disposable sensing heads in a biosensor. The majority of all published work concerning surface-plasmon-based biosensors has detailed the use of prisms as the momentum-coupling devices. Therefore, the relative advantages and disadvantages of both systems are discussed in this paper, including a sensitive optical technique which is beyond the scope of prism geometries.
Lawrence
C R
CR
Division of Chemicals & Polymers, CSIRO, Clayton, Victoria, Australia.
Geddes
N J
NJ
Furlong
D N
DN
D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001703:Biophysics / Q000379:methods*; D015374:Biosensing Techniques; D004209:Disposable Equipment; D006696:Holography / Q000295:instrumentation; D007074:Immunoglobulin G / Q000276:immunology*; D011485:Protein Binding
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G