title: Complement-derived anaphylatoxins in human donor corneas treated with excimer laser.
abstract: OBJECTIVE
  An inflammatory response produced by excimer laser photorefractive keratectomy (PRK) may be associated with the subsequent corneal haze and regressions in refractive error observed after treatment. Complement-derived anaphylatoxins, potent mediators of inflammation, may have a role in postoperative healing.
  
  
  METHODS
  Twenty right human donor corneas underwent a 6-D excimer laser PRK treatment. The corresponding left donor corneas served as the controls. After incubation in tissue culture media for 6 hours and elution in phosphate-buffered saline with EDTA for 24 hours, complement-derived anaphylatoxins C3a, C4a, and C5a were measured in corneal eluates by radioimmunoassay.
  
  
  RESULTS
  Compared with control corneas, the excimer PRK corneas failed to demonstrate a significant increase in C3a, C4a, or C5a levels (P > .05).
  
  
  CONCLUSIONS
  These results suggest that the excimer laser at this dose does not activate significant complement in the cornea.
authors: Department of Ophthalmology, UCLA School of Medicine, USA.
authors: B P
authors: BP
authors: Gardner
authors: U
authors: U
authors: Pleyer
authors: B J
authors: BJ
authors: Mondino
authors: H L
authors: HL
authors: Sumner
authors: M L
authors: ML
authors: Frieberg
authors: P S
authors: PS
authors: Imperia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000706:Anaphylatoxins / Q000096:biosynthesis*; D002648:Child; D003167:Complement Activation; D015926:Complement C3a / Q000032:analysis; D015934:Complement C4a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D003315:Cornea / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D006801:Humans; D054018:Lasers, Excimer; D008875:Middle Aged; D018903:Photorefractive Keratectomy; D011863:Radioimmunoassay
chemical_list: D000706:Anaphylatoxins; D015926:Complement C3a; D015934:Complement C4a; D015936:Complement C5a