Cyclosporine A shows immunosuppressor activity on T lymphocytes from the peripheral blood and synovial fluid of patients with autoimmune arthritis.
OBJECTIVE
This work studies the effects of Cyclosporine A (CsA) upon the activation and proliferation of mononuclear cells (MNC) from the peripheral blood (PB) of patients with chronic autoimmune arthritis and from healthy controls, and from the synovial fluid (SF) of patients.


METHODS
In vitro studies of activation, proliferation, mRNA expression and lymphokine production were carried out.


RESULTS
We found in the PB and SF MNCs from patients with autoimmune arthritis that CsA inhibits the proliferative response, activation antigen expression, IL-2 mRNA expression and IL-2 production induced by polyclonal mitogens in a dose dependent manner.


CONCLUSIONS
CsA blocks lymphocyte activation in PB and SF MNCs from patients with autoimmune arthritis.
Guillen
C
C
Servicio de Enfermedades del Sistema Immune, Hospital Universitario Principe de Asturias, Spain.
Prieto
A
A
Perez-Blas
M
M
Alvarez de Buergo
M
M
Villa
L
L
Rico
H
H
Zea
A
A
Alvarez-Mon
M
M
D000941:Antigens / Q000032:analysis; D001168:Arthritis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D001483:Base Sequence; D001773:Blood Cells / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D016572:Cyclosporine / Q000627:therapeutic use*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007376:Interleukin-2 / Q000235:genetics; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*
D000941:Antigens; D016207:Cytokines; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D015335:Molecular Probes; D012333:RNA, Messenger; D016572:Cyclosporine