title: Molecular cloning and expression of alpha parvalbumin in the guinea pig cochlea.
abstract: We have cloned and sequenced an alpha parvalbumin cDNA from the guinea pig cochlea. The deduced amino acid sequence shows greater identity with the rabbit sequence (86.3%) than with other mammalian sequences (< 82%). Using in situ hybridization and immunohistochemistry, alpha parvalbumin mRNA and protein were found in primary auditory neurons and inner hair cells, in agreement with RT-PCR data showing alpha parvalbumin mRNA expression in the spiral ganglion and the organ or Corti.
authors: Neurobiologie de l'Audition-Plasticité Synaptique, INSERM U 254, Université de Montpellier.
authors: A
authors: A
authors: Soto-Prior
authors: M
authors: M
authors: Cluzel
authors: N
authors: N
authors: Renard
authors: C
authors: C
authors: Ripoll
authors: M
authors: M
authors: Lavigne-Rebillard
authors: M
authors: M
authors: Eybalin
authors: C P
authors: CP
authors: Hamel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003051:Cochlea / Q000378:metabolism*; D018076:DNA, Complementary / Q000302:isolation & purification*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D010320:Parvalbumins