title: A glucokinase-like-enzyme in the liver of Atlantic salmon (Salmo salar).
abstract: An enzyme with properties similar to rat liver glucokinase (Hexokinase IV or D) is present in salmon liver in addition to low-Km hexokinase(s). The specific activity of this enzyme increases about 1.6 fold, comparing activities after feeding diets with 25% and 0% digestive energy from starch. The enzyme has a low affinity for glucose, S0.5 = 25.2-26.8 mM (95% confidence interval) and a low activity with fructose, approximately 8% of the activity with glucose. Its molecular mass was estimated to 50.7 +/- 0.6 kDa (SEM. n = 3) by gel filtration, and it displays positive cooperativity with respect to glucose. The Hill constant = 1.73-1.81 (95% confidence interval). The enzyme is competitively inhibited by N-acetyl glucosamine, K(i) approximately 0.28 mM.
authors: Department of Biochemistry, Physiology and Nutrition, Norwegian College of Veterinary Medicine, Oslo, Norway.
authors: M A
authors: MA
authors: Tranulis
authors: O
authors: O
authors: Dregni
authors: B
authors: B
authors: Christophersen
authors: A
authors: A
authors: Krogdahl
authors: B
authors: B
authors: Borrebaek
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005941:Glucokinase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000737:chemistry / Q000378:metabolism; D006003:Glycogen / Q000032:analysis / Q000378:metabolism; D006593:Hexokinase / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000737:chemistry / Q000201:enzymology*; D008970:Molecular Weight; D012474:Salmon / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D006003:Glycogen; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D006593:Hexokinase; D005941:Glucokinase; D005947:Glucose; D000117:Acetylglucosamine