title: Expression of the CTL-associated protein TIA-1 during murine embryogenesis.
abstract: TIA-1 is a T cell-associated protein that binds poly(A) in vitro and induces apoptosis in permeabilized thymocytes. It may be involved in the induction of apoptosis in target cells during lymphocyte attack. To elucidate the role of TIA-1 in mammalian development, a cDNA-encoding mouse TIA-1 was cloned. The predicted mouse TIA-1 protein contains three RNA binding domains at the amino terminus and a putative lysosomal targeting sequence at the carboxyl terminus. The mouse sequence shows 96% overall identity with the human TIA-1 homologue. During murine embryogenesis, abundant mouse TIA-1 mRNA is detectable from 12.5 days of development onward in the brain and the retina, where it is selectively expressed within neuronal cells. Transcripts are also found in the lung, kidney, and thymus. TIA-1 in the adult mouse is expressed mainly in T cells and NK cells. The expression of TIA-1 during mouse embryogenesis is endogenous to tissues in which apoptotic cell death occurs. The conservation of this RNA-binding protein throughout evolution implicates its importance in embryogenesis, and in particular neuronal development.
authors: Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors: B
authors: B
authors: Lowin
authors: L
authors: L
authors: French
authors: J C
authors: JC
authors: Martinou
authors: J
authors: J
authors: Tschopp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000235:genetics; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005326:Fetal Proteins / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D012160:Retina / Q000196:embryology / Q000378:metabolism; D016415:Sequence Alignment; D000074019:T-Cell Intracellular Antigen-1; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D005136:Eye Proteins; D005326:Fetal Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; C499367:Tia1 protein, mouse