title: Inhibition of oxidative modification of proteins by RU486.
abstract: OBJECTIVE
  To elucidate further the antioxidant properties of RU486. We determined whether it can protect biologic molecules such as proteins (albumin, low-density lipoprotein [LDL] and oxidized LDL) from damage by pre-existing lipid peroxides.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  We tested the effects of RU486 on the formation of fluorescent oxidatively modified proteins by pre-existing lipid peroxides. We used two model systems, the incubation of oxidized linoleic acid with serum albumin and the incubation of human LDL with copper.
  
  
  METHODS
  The formation of modified protein was established by determining fluorescence at excitation wavelength of 330 nm and emission wavelength between 390 and 500 nm. Modified protein has a characteristic emission between 425 and 430 nm.
  
  
  RESULTS
  The addition of increasing amounts of RU486 inhibited the formation of fluorescent oxidatively modified protein products in both model systems.
  
  
  CONCLUSIONS
  These results provide evidence that RU486 not only can prevent the formation of lipid peroxide, but also can block the formation of fluorescent protein adducts in the presence of pre-existing lipid peroxides.
authors: Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors: S E
authors: SE
authors: Carpenter
authors: N
authors: N
authors: Santanam
authors: A A
authors: AA
authors: Murphy
authors: J A
authors: JA
authors: Rock
authors: S
authors: S
authors: Parthasarathy
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D008041:Linoleic Acids / Q000378:metabolism*; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL / Q000378:metabolism*; D015735:Mifepristone / Q000494:pharmacology*; D012709:Serum Albumin / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D008041:Linoleic Acids; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D012709:Serum Albumin; C038649:13-hydroperoxy-9,11-octadecadienoic acid; D015735:Mifepristone; D003300:Copper