[Effects of assisted ovarian stimulation with leuprolide acetate on alterations of plasma estradiol levels and newly synthesized endometrial proteins].
OBJECTIVE
To evaluate the effects of leuprolide acetate (LA)-assisted ovarian stimulation on endometrial protein synthesis in hamsters.


METHODS
Hamsters were treated separately with three different regimens: (1) 40 IU of pregnant mare's serum gonadotropin (PMSG) followed by 100 IU of hCG after 52 hours (PMSG alone group). (2) Same volumes of normal saline on the same schedule (control group). (3) 40 micrograms of LA daily prior to the same regimen of PMSG-hCG treatment in order to down-regulate the pituitary-ovarian functions (LA-PMSG group). The newly synthesized endometrial proteins were characterized and quantitated in vitro by 3H-leucine incorporation test and 10% SDS-PAGE technique.


RESULTS
The levels of newly synthesized 3H-labelled endometrial proteins were not different between LA-PMSG and control groups. However, those of low and middle molecular weight (< 31,000 and 45,000) proteins in the PMSG alone group were significantly lower than the levels in both LA-PMSG and control groups (P < 0.05, P < 0.01).


CONCLUSIONS
The addition of LA in ovarian stimulation protocol resulted in more physiological endometrial protein synthesis. This phenomenon may causally related to higher pregnancy rate when applied in vitro fertilization-embryo transfer programs.
Huang
H
H
Obstetrics and Gynecology Hospital, Zhejiang Medical University, Hangzhou.
Shi
Y
Y
Zhou
F
F
D000818:Animals; D006224:Cricetinae; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000097:blood*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D016729:Leuprolide / Q000494:pharmacology*; D008647:Mesocricetus; D010062:Ovulation Induction; D014176:Protein Biosynthesis
D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D016729:Leuprolide