[Comparative experiment of gas chromatography determination of Mycobacterium bacillus].
Using capillary column gas chromatography (GC) technique for detecting cellular fatty acid, the test of the GC chromatograms of 26 reference Mycobacteria strains (freeze-dried strains and transfer growed strains at the improved Lowenstein-Jensen culture for three or four weeks) was performed. On the basis of the test, a comparison was made between the GC method and the conventional method for determination of 54 strains of non-tuberculous mycobacteria which were separated from clinical specimens, and the conformational rate of the two methods was 94 percent. At the same time, the relationship between the drug resistance and the changes of GC chromatograms of M. tuberculosis and M. bovis which were separated from clinical samples was observed. The results demonstrated that the GC method would achieve better results if combined with the bacterial growing speed, the condition of producing colour and the condition of growing at PNB and TCH culture during determining the mycobacterium strains which were separated from clinical specimens.
Zhai
B
B
Hebei Provincial Chest Hospital, Shijiazhuang.
Zhang
L
L
Yan
X
X
D002849:Chromatography, Gas; D004352:Drug Resistance, Microbial; D005227:Fatty Acids / Q000032:analysis; D009163:Mycobacterium bovis / Q000737:chemistry / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000302:isolation & purification*
D005227:Fatty Acids