Distribution of platelet activating factor receptor mRNA along the rat nephron segments.
Platelet activating factor (PAF) receptor mRNA was semi-quantitated in microdissected rat nephron segments by the reverse transcription-polymerase chain reaction (RT-PCR). PCR primers were designed from two alternative splicing isoforms of rat PAF receptor cDNA; a homologue of human leukocyte PAF receptor cDNA (p172) and the other with a unique sequence in the 5'-noncoding region upstream of the splice acceptor site (p18). Only the expression of mRNA corresponding to p172 was detectable, which was most abundant in the glomerulus, followed in order by: proximal convoluted tubule > proximal straight tubule > outer medullary and cortical collecting duct, distal convoluted tubule > thick ascending limb of Henle's loop. These results suggest the potential physiological roles of PAF in the entire renal tubule as well as in the glomerulus.
Asano
K
K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Taniguchi
S
S
Nakao
A
A
Watanabe
T
T
Kurokawa
K
K
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D008969:Molecular Sequence Data; D009399:Nephrons / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled
D017931:DNA Primers; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor