Comparison of two methods to obtain hemizonae pellucidae for sperm function tests.
The development of a new generation of diagnostic techniques has provided objective data on the physiological function of the spermatozoon. The hemizona assay has been considered the best predictor for in-vitro fertilization results. Its clinical application has been limited to the availability of human oocytes and the use of a special micromanipulator. Here, the sperm binding capacity of hand-sectioned hemizonae was compared with that of oocytes bisected by a micromanipulator. The results obtained in parallel assays showed no statistical differences between the two methods. Therefore, the bisection of oocytes by hand is useful for hemizona assays even in normal clinical laboratories.
Sánchez
R
R
Department of Dermatology and Andrology, Justus Liebig, University, Giessen, Germany.
Finkenzeller
C
C
Schill
W B
WB
Miska
W
W
D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008866:Microsurgery / Q000379:methods; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology