Electrophoretic analysis of biliary proteins: application of high resolution two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradient in the first dimension.
Native bile samples, dialyzed against double distilled water, fractionated by Sepharose 2B column chromatography, concentrated by ultrafiltration and lyophilized, were solubilized in lysis buffer and studied by two-dimensional electrophoresis (2-DE) with immobilized pH gradients in the first dimension. We counted 70, and 7 spots in 2-DE patterns of bile from patients with cholesterol gallstones and pigment gallstones, respectively.
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Department of Medicine II, Klinikum Grosshadern, Ludwig-Maximilians-University Munich, Germany.
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D001646:Bile / Q000737:chemistry*; D002769:Cholelithiasis / Q000378:metabolism / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining
D011506:Proteins