title: A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8.
abstract: We generated a panel of 97 radiation hybrids from the cell line GM10156B, which contains only human chromosome 8 in a Chinese hamster ovary cell line background. Statistical analysis of the cosegregation of markers in the 97 radiation hybrids was used to construct a physical map delineating the order and intermarker distance of 40 8q24 loci. Twenty-one loci were ordered with maximum likelihood ratios greater than 1000:1. A high level of consistency was seen between our RH map and the published genetic map, suggesting that our panel will be a valuable resource for the rapid mapping of markers derived from human chromosome 8.
authors: Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284, USA.
authors: T B
authors: TB
authors: Lewis
authors: L
authors: L
authors: Nelson
authors: K
authors: K
authors: Ward
authors: R J
authors: RJ
authors: Leach
mesh_terms: D000228:Adenine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D006224:Cricetinae; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase