Internalization and biological effects of serum albumin in the breast cancer MCF-7 and MDA-MB 231 cells.
We show that albumin is internalized by human breast cancer cells (MDA-MB 231 and MCF-7) in culture by using confocal laser scanning microscopy. Moreover, albumin has an effect on the level of radioactivity incorporated when the cells are incubated with radioactive estradiol, and it is necessary to observe the mitogenic effect of estradiol towards the MCF-7 cells. This finding opens some possibilities regarding the internalization mechanisms and fate (degradation, recycling) of albumin as well as the role played by this protein in the intracellular metabolism of estradiol and in the intra-extracellular traffic of estradiol and its metabolites.
Germain
P
P
Laboratoire de Biochimie, Faculté de Pharmacie, Limoges, France.
Metezeau
P
P
Hellio
R
R
Habrioux
G
G
D001693:Biological Transport, Active / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D018613:Microscopy, Confocal; D008934:Mitogens / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008934:Mitogens; D012710:Serum Albumin, Bovine; D004958:Estradiol