Association of CD24 with the kinase c-fgr in a small cell lung cancer cell line and with the kinase lyn in an erythroleukemia cell line.
Human CD24 is a highly glycosylated glycosylphosphatidylinositol-linked (GPI-linked) cell surface protein. GPI-linked proteins are involved in signal transduction mediated by members of the protein tyrosine kinase (PTK) family. Therefore we studied associated molecules providing the signaling capacity of CD24. Lysates of SW2 and K562 cells were analysed for expression of PTK of the c-src family by Western blotting. We identified c-fgr in SW2 lysates and c-fgr and also lyn in K562 lysates. To study a putative association of these PTK with CD24 we performed immunoprecipitations with the mAb SWA11 directed against CD24. Western analysis of the precipitates showed an association of c-fgr with CD24 in SW2 cells and lyn in K562 cells. We conclude that either c-fgr or lyn is physically associated with CD24 in a cell-type depending manner. An involvement of these complexes in signaling phenomenons of CD24 in small cell lung cancer and in leukaemias is discussed.
Zarn
J A
JA
Department of Medicine, University Hospital, Zürich, Switzerland.
Zimmermann
S M
SM
Pass
M K
MK
Waibel
R
R
Stahel
R A
RA
D015703:Antigens, CD / Q000378:metabolism*; D051927:CD24 Antigen; D018288:Carcinoma, Small Cell / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000378:metabolism*; D008562:Membrane Glycoproteins; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D019061:src-Family Kinases / Q000378:metabolism*
D015703:Antigens, CD; D051927:CD24 Antigen; C497889:CD24 protein, human; D008562:Membrane Glycoproteins; D011518:Proto-Oncogene Proteins; C060902:lyn protein-tyrosine kinase; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases