Neutron scattering in the plane of membranes: structure of alamethicin pores.
A technique of neutron in-plane scattering for studying the structures of peptide pores in membranes is described. Alamethicin in the inserted state was prepared and undeuterated and deuterated dilauroyl phosphatidylcholine (DLPC) hydrated with D2O or H2O. Neutron in-plane scattering showed a strong dependence on deuteration, clearly indicating that water is a part of the high-order structure of inserted alamethicin. The data are consistent with the simple barrel-stave model originally proposed by Baumann and Mueller. The theoretical curves computed with this model at four different deuteration conditions agree with the data in all cases. Both the diameter of the water pore and the effective outside diameter of the channel are determined accurately. Alamethicin forms pores in a narrow range of size. In a given sample condition, > 70% of the peptide forms pores of n and n +/- 1 monomers. The pore size varies with hydration and with lipid. In DLPC, the pores are made of n = 8-9 monomers, with a water pore approximately 18 A in diameter and with an effective outside diameter of approximately 40 A. In diphytanoyl phosphatidylcholine, the pores are made of n approximately 11 monomers, with a water pore approximately 26 A in diameter, with an effective outside diameter of approximately 50 A.
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Physics Department, Rice University, Houston, Texas 77005-1892, USA.
Ludtke
S J
SJ
Worcester
D L
DL
Huang
H W
HW
D000408:Alamethicin / Q000737:chemistry*; D000595:Amino Acid Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D007473:Ion Channels / Q000737:chemistry; D008567:Membranes, Artificial; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009502:Neutrons; D012542:Scattering, Radiation
D007473:Ion Channels; D008567:Membranes, Artificial; D000408:Alamethicin