Potential contribution of optional urease-positive bacteria to idiopathic urinary calcium stone formation. II. Microlith formation kinetics in a fermenter model of the urinary tract infected by optional urease-positive microorganisms.
We investigated the effects of weak to moderate urease hydrolysis by optional urease-positive microorganisms in an artificial urine model enriched with calcium phosphate and calcium oxalate in respect of calcium stone formation. The incubation experiments were performed using a discontinuously running fermenter device to simulate the urinary system. The kinetics of cell division rates, pH and ammonium ion production were measured and correlated to crystallite appearance in the incubation medium. Qualitative analyses of the sediments revealed apatite. Investigations using light microscopy and scanning electron microscopy (SEM) confirmed the matrix effect of bacterial glycoproteins. It was shown that initiation of calcium oxalate stone formation is in all probability equally determined by matrix effects and by heteronuclear crystallization if the urinary tract is infected by optional urease-positive bacteria. When urinary inorganic phosphate is present, calcium phosphate nidi are always initially formed, and may subsequently be coated by calcium oxalate.
Leusmann
D B
DB
St. Hildegardis Hospital, Cologne, Germany.
Sabinski
F
F
D002129:Calcium Oxalate / Q000652:urine; D002130:Calcium Phosphates / Q000652:urine; D003460:Crystallization; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D011513:Proteus mirabilis / Q000201:enzymology*; D000644:Quaternary Ammonium Compounds / Q000652:urine; D014510:Urease / Q000032:analysis*; D014545:Urinary Calculi / Q000382:microbiology* / Q000648:ultrastructure; D014552:Urinary Tract Infections / Q000378:metabolism*
D002130:Calcium Phosphates; D000644:Quaternary Ammonium Compounds; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002129:Calcium Oxalate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D014510:Urease; C485829:calcium phosphate, dibasic, anhydrous