title: Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase.
abstract: The nucleotide sequence of the large (L) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) was determined, completing the first entire genome sequence of a nairovirus. The L segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (M) and small (S) genomic segments. A single open reading frame (ORF) was present in the viral complementary strand, sufficient to encode a protein of 459 kDa. The predicted protein sequence showed the core polymerase motifs characteristic of the RNA-dependent RNA polymerases of segmented negative-stranded viruses. Comparison of the conserved motifs with the corresponding region of other segmented negative-strand viruses showed a closer relationship between nairoviruses and phleboviruses than with other Bunyaviridae or with other virus families. However, the core polymerase was the only function that could be assigned to a region of the DUG L gene.
authors: NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
authors: A C
authors: AC
authors: Marriott
authors: P A
authors: PA
authors: Nuttall
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008969:Molecular Sequence Data; D016855:Nairovirus / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases