[Inducing effect of dimethy-4, 4'-dimethoxy-5, 6,5',6-dimethylenedioxybipheny-2, 2'-dicarboxylate (DDB) on differentiation of leukemia HL-60 cells].
OBJECTIVE
To study the effects of anti-hepatitis drug, DDB, on leukemia cell line HL-60.


METHODS
Cytobiological methods were used.


RESULTS
DDB was inhibited the proliferation of HL-60 cells. About 50% of HL-60 cells treated with DDB (10(-4)mol/L) for 6 days exhibited NBT reduction, and phagocytosis activity was also enhanced by DDB (10(-4)mol/L) for 4 days. The HL-60 cells treated with DDB turned out to be mature granulocytes morphologically.


CONCLUSIONS
The activity of acid phosphatase in DDB-treated HL-60 cells was significantly increased. DDB can induce HL-60 cells to differentiate along granulocyte lineage.
Liu
Z
Z
Department of Pharmacology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Cui
Q
Q
Fu
T
T
D000135:Acid Phosphatase / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004149:Dioxoles / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D010587:Phagocytosis / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D004149:Dioxoles; C041398:7,7'-dimethoxy-(4,4'-bi-1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D000135:Acid Phosphatase