title: Swelling and cAMP on hyperpolarization-activated Cl- conductance in rat Leydig cells.
abstract: We have used the whole cell patch-clamp technique to characterize changes in membrane conductance induced by osmotic swelling in mature rat Leydig cells dialyzed with ATP (control cells) or adenosine 3',5'-cyclic monophosphate (cAMP) plus ATP (cAMP cells). A spontaneous current activation occurs in both groups in isosmotic conditions (300/295 mosM in/out). This development is entirely counteracted in control cells and partly inhibited in cAMP cells by exposing them to a hyperosmotic (350 mosM) bath solution, and these currents increase again in a hyposmotic (205 mosM) bath solution. These currents are sensitive to 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, a Cl- channel blocker. Taken together, the results indicate that, in the control cells (ATP alone) as well as in the presence of intracellular cAMP, osmotic swelling activates the background hyperpolarization-activated Cl- conductance, osmotic swelling and cAMP appearing to act synergistically.
authors: Laboratoire de Physiologie Animale, Unité de Recherche Associée Centre, National de la Recherche Scientifique 1869, Poitiers, France.
authors: J F
authors: JF
authors: Noulin
authors: E
authors: E
authors: Fayolle-Julien
authors: J F
authors: JF
authors: Desaphy
authors: J P
authors: JP
authors: Poindessault
authors: M
authors: M
authors: Joffre
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002712:Chlorides / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology*; D004553:Electric Conductivity; D004594:Electrophysiology; D007038:Hypotonic Solutions / Q000494:pharmacology; D007700:Kinetics; D007985:Leydig Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D051381:Rats; D012996:Solutions / Q000494:pharmacology
chemical_list: D002712:Chlorides; D007038:Hypotonic Solutions; D012996:Solutions; D000242:Cyclic AMP