title: Comparative evaluation of p53-protein expression and the PCNA and Ki-67 proliferating cell indices in human astrocytomas.
abstract: Mutations of the p53 gene are one of the most frequent genomic alterations of human tumours of astrocytic lineage. Because the physiological role of this gene is a suppression of cellular proliferation and growth, the overexpression of p53-protein may correlate with the expression of PCNA or Ki-67, established markers of cell proliferation. Paraffin-embedded surgical specimens from 60 human astrocytomas (9 pilocytic tumours, 12 WHO grade II, 9 anaplastic astrocytomas [WHO grade III] and 30 glioblastomas [WHO grade IV]) were stained with anti-PCNA (PC10), anti-p53(DO-7) and anti-Ki-67 antibodies (DAKO). Approximately 40% of all the cases were p53-protein immunopositive (53.3% glioblastomas, 33.3% anaplastic, 41.7% low grade astrocytomas but no pilocytic tumor). Statistical analysis did not reveal statistically significant correlation between p53-immunopositivity and PCNA or Ki-67 labeling indices. The Ki-67- and PCNA LI-s were statistically correlated, and the former better discriminated groups of different grades of malignancy.
authors: Department of Oncology, Medical University of Lodz, Poland.
authors: R
authors: R
authors: Kordek
authors: W
authors: W
authors: Biernat
authors: M
authors: M
authors: Debiec-Rychter
authors: J
authors: J
authors: Alwasiak
authors: P P
authors: PP
authors: Liberski
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D001254:Astrocytoma / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D001932:Brain Neoplasms / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D005909:Glioblastoma / Q000276:immunology; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D008940:Mitotic Index / Q000276:immunology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis* / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53