RNA-primed PCR.
We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.
Shibata
H
H
Division of Genome Analysis, Institute of Genetic Information, Kyushu University, Fukuoka, Japan.
Tahira
T
T
Hayashi
K
K
D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005090:Exodeoxyribonucleases / Q000378:metabolism; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012313:RNA; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009843:Oligoribonucleotides; C034477:RNA primers; D012313:RNA; C093010:DNA polymerase, Thermus thermophilus; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases