A microdialysis fibre based sampler for flow injection analysis: determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor.
A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilised in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.
Palmisano
F
F
Dipartimento di Chimica, Università degli Studi via Orabona, Bari, Italy.
Centonze
D
D
Quinto
M
M
Zambonin
P G
PG
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry; D017022:Flow Injection Analysis / Q000379:methods*; D005504:Food Analysis; D006801:Humans; D007773:Lactates / Q000032:analysis* / Q000097:blood; D019344:Lactic Acid; D008567:Membranes, Artificial; D017551:Microdialysis; D008892:Milk / Q000737:chemistry; D008970:Molecular Weight; D011786:Quality Control
D007773:Lactates; D008567:Membranes, Artificial; D019344:Lactic Acid