[A study on DNA content and cell cycle phase analysis in endometrial carcinoma].
OBJECTIVE
To study the prognostic value of DNA content, cell cycle phase in endometrial carcinoma.


METHODS
DNA content and cell cycle phase analysis were performed on 100 paraffin-embedded archival specimens from 103 cases of endometrial carcinoma using flow cytometry.


RESULTS
Aneuploidy was found in 71.0% of specimens. The aneuploidy rate in specimens from grade III, deep myometrial invasion and stage III, IV was significantly higher than that in specimens from grade I, nonmyometrial invasion and stage I (P < 0.05 respectively). 5-year survival rate for patients with aneuploidy was 65.9% and for patients with diploidy was 87.6% (P < 0.01). High S-phase fraction (SPF) was associated with high grade, deep myometrial invasion and advanced stage. The mean SPF was 13.4% in the living group and 17% in the dead group. The 5-year survival rate for patients with SPF > 17% was 50.4% and for patients with SPF < 17% was 79.1% (P < 0.01). SPF and stage were the most important prognostic factors in endometrial carcinoma by Cox's proportional hazards model.


CONCLUSIONS
DNA ploidy and SPF were found to be of important prognostic indicators in endometrial carcinoma.
Xue
F
F
General Hospital of Tianjin Medical University.
Jiao
S
S
Zhao
F
F
D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality; D000782:Aneuploidy; D004273:DNA, Neoplasm / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D016196:S Phase; D015996:Survival Rate
D004273:DNA, Neoplasm