Epidemiological typing of Flavimonas oryzihabitans by PCR and pulsed-field gel electrophoresis.
Flavimonas oryzihabitans has emerged as a potential nosocomial pathogen in recent years. The typing method for characterization of this species has never been reported before. Pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC)-based PCR were used to generate DNA fingerprints for 14F. oryzihabitans isolates obtained from eight episodes of nosocomial infections during a 2-year period. Both techniques successfully classified these clinical isolates into eight distinct genotypes, thus indicating that all of these episodes of infections were independent. In contrast, repeated isolates from the same patient were assigned to identical genotypes. The reproducibility of both techniques was good. Therefore, we conclude that both PFGE and ERIC-PCR have comparable reproducible and discriminatory powers for the typing of F. oryzihabitans and may be useful for clarifying the epidemiology of this species; however, ERIC-PCR has the advantages of both speed and simplicity.
Liu
P Y
PY
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, Republic of China.
Shi
Z Y
ZY
Lau
Y J
YJ
Hu
B S
BS
Shyr
J M
JM
Tsai
W S
WS
Lin
Y H
YH
Tseng
C Y
CY
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002648:Child; D003428:Cross Infection / Q000453:epidemiology / Q000209:etiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005069:Evaluation Studies as Topic; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000209:etiology; D015203:Reproducibility of Results; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial