title: Urease activity does not contribute dramatically to persistence of Mycobacterium bovis bacillus Calmette-Guérin.
abstract: Multiplication of BCGure-, an isogenic urease-negative mutant of Mycobacterium bovis BCG constructed by allelic exchange (J. M. Reyrat, F. X. Berthet, and B. Gicquel, Proc. Natl. Acad. Sci. USA 92:8768-8772, 1995), was examined in human macrophages and mice. Although ureolytic activity was not essential to BCGure-growth, a slight decrease in the multiplication and persistence of the mutated strain compared with wild-type BCG was observed in lungs of infected mice.
authors: Unité de Génétique Mycobactérienne (CNRS, URA 1300), Institut Pasteur, Paris, France.
authors: J M
authors: JM
authors: Reyrat
authors: G
authors: G
authors: Lopez-Ramirez
authors: C
authors: C
authors: Ofredo
authors: B
authors: B
authors: Gicquel
authors: N
authors: N
authors: Winter
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009163:Mycobacterium bovis / Q000201:enzymology* / Q000254:growth & development; D013154:Spleen / Q000382:microbiology; D014510:Urease / Q000502:physiology*
chemical_list: D014510:Urease