Effects of acute n-hexane and 2,5-hexanedione treatment on the striatal dopaminergic system in mice.
In order to investigate the effect of n-hexane and its metabolites on the Central Nervous System (CNS), we treated mice with n-hexane and 2,5-hexanedione (2,5-HD) by intraperitoneal (i.p.) administration. Gascromatographic mass spectrometric (GCMS) analyses of striatum and cerebellum revealed a consistent increase of 2,5-HD concentration at 0.5 and 2 hours after treatment and a decline to baseline levels at 24 hours. Traces of 2,5-HD were detected in the brain of control animals. Biochemical analyses revealed a precocious, short lasting, significant increase of striatal dopamine (DA) and homovanillic acid (HVA) levels. A significant increase of striatal synaptosomal DA uptake, suggesting a DA releasing effect on the dopaminergic terminals, was also observed. These results support the hypothesis of a possible role of n-hexane and its metabolites in inducing parkinsonism in humans and animals.
Masotto
C
C
1Zambon Group, Bresso Milan, Italy.
Bisiani
C
C
Camisasca
C
C
Fusi
R
R
Ricciardi
S
S
Fonzi
N
N
Perbellini
L
L
Scatturin
M
M
Mariani
C B
CB
Canesi
M
M
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D006586:Hexanes / Q000494:pharmacology*; D006588:Hexanones / Q000494:pharmacology*; D006719:Homovanillic Acid / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013997:Time Factors
D006586:Hexanes; D006588:Hexanones; D015102:3,4-Dihydroxyphenylacetic Acid; C026385:n-hexane; C011269:2,5-hexanedione; D004298:Dopamine; D006719:Homovanillic Acid