A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
Buchholz
F
F
European Molecular Biology Laboratory, Gene Expression Program, Heidelberg, Germany.
Angrand
P O
PO
Stewart
A F
AF
D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D019426:Integrases; D010957:Plasmids; D011995:Recombination, Genetic; D014764:Viral Proteins
D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases