The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 A resolution.
The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial number of T cells. The crystal structure of this protein has been refined to 2.07 A with an Rcryst value of 20.4% for 51,240 reflections. The final model contains three molecules in the asymmetric unit with good stereochemistry and a root-mean-square deviation of 0.009 A and 1.63 from ideality for bond lengths and bond angles, respectively. The overall fold is considerably similar to that of other known microbial superantigens (staphylococcal enterotoxins). However, a detailed structural analysis shows that toxic shock syndrome toxin-1 lacks several structural features that affect its specificity for V beta elements of the T-cell receptor and also its recognition by major histocompatibility class II molecules.
Papageorgiou
A C
AC
School of Biology and Biochemistry, University of Bath, Claverton Down, UK.
Brehm
R D
RD
Leonidas
D D
DD
Tranter
H S
HS
Acharya
K R
KR
D000595:Amino Acid Sequence; D001427:Bacterial Toxins; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004768:Enterotoxins / Q000737:chemistry* / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000737:chemistry / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000378:metabolism; D018089:Superantigens / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001427:Bacterial Toxins; D004768:Enterotoxins; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D014867:Water; C034959:enterotoxin C, staphylococcal