Effects of the passive transfer of anti-gB antibodies in a rabbit model of HSV-1-induced keratitis.
The effectiveness of passively transferred antibodies directed against the secretory form of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB1-s) was tested in a rabbit model of ocular HSV-1 infection. The animals were passively immunized through the intramuscular injection of a homologous polyclonal anti-gB1-s antiserum at different times from the viral ocular challenge (i.e. at -24, 0, +24 and +48 h from infection). The effects observed in this trial were compared with those obtained in an active immunization trial, in which the animals were vaccinated with gB1-s before the ocular infection with HSV-1 (large variant). The results have shown that passive immunization appears quite effective in prophylactic utilization, whereas it is less effective when performed at 24 or 48 h after inoculation. By contrast, active immunization of rabbits proved to be highly effective both in preventing the development of fatal encephalitis and in reducing the severity of corneal lesions.
Incorvaia
C
C
Department of Clinical Ophthalmology, University of Ferrara, Italy.
Manservigi
R
R
Parmeggiani
F
F
Monini
P
P
Longhini
L
L
Caselli
E
E
Cassai
E
E
D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage* / Q000032:analysis; D003315:Cornea / Q000821:virology; D004195:Disease Models, Animal; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000276:immunology*; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000032:analysis; D007273:Injections, Intramuscular; D016849:Keratitis, Herpetic / Q000503:physiopathology / Q000517:prevention & control*; D011817:Rabbits; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus