DNA fingerprinting confirms isogenicity of androgenetically derived rainbow trout lines.
Homozygous and hybrid clonal lines of rainbow trout (Oncorhynchus mykiss) were confirmed to be isogenic using multilocus DNA fingerprinting. Homozygous clones were produced by androgenesis and gynogenesis using gametes from androgenetic male and female rainbow trout, respectively. Isogenic F1 hybrid lines were produced by crossing homozygous fish from different strains. One line of hybrid clones showed segregation for maternally inherited DNA fingerprint markers. The female from this cross, the only presumptive homozygous gynogenetic individual used in this study, was thought to have been produced by gynogenesis followed by blockage of the first cleavage division, but based on the DNA fingerprint analysis, apparently was derived by spontaneous polar body retention that maintained heterozygosity at some loci. Mutations at DNA fingerprint loci were not observed, indicating relative stability of fingerprint loci in the clonal lines. DNA fingerprinting appears to be a useful tool for identifying and genetically monitoring clonal lines of rainbow trout. Isogenic lines of rainbow trout will facilitate the production of saturated genetic maps for rainbow trout and enhance such endeavors as quantitative trait locus (QTL) analysis and loss of heterozygosity (LOH) studies in tumors.
Young
W P
WP
Department of Zoology, Washington State University, Pullman 99164-4236, USA.
Wheeler
P A
PA
Fields
R D
RD
Thorgaard
G H
GH
D000728:Androgens / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D015342:DNA Probes; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*
D000728:Androgens; D015342:DNA Probes