title: UVA-induced immune suppression in human skin: protective effect of vitamin E in human epidermal cells in vitro.
abstract: UVA (320-400 nm) radiation damage to membranes, proteins, DNA and other cellular targets is predominantly related to oxidative processes. In the present study, we demonstrated that cutaneous UVA-induced immunosuppression can be related, at least in part, to the appearance of these oxidative processes. The UVA-induced oxidative processes in freshly isolated epidermal cells were monitored by measuring the thiobarbituric acid reactive substances (TBARS) as an index of peroxidation. The in vitro immunosuppressive effects of UVA were demonstrated by measuring the allogeneic lymphocyte proliferation induced by epidermal cells or purified Langerhans cells in the mixed epidermal cell-lymphocyte reaction (MECLR). In addition, the effects of a potent antioxidant (vitamin E) on these two UVA-induced processes were analysed. Our results showed that the antigen-presenting function of Langerhans cells measured in the MECLR is dose-dependently decreased by UVA radiation (up to 20J/cm2). Overnight incubation of epidermal cells with vitamin E (400 mumol/l) before irradiation partially protected epidermal cells from the immunosuppressive effects of UVA radiation, and decreased TBARS release into the supernatant (a decrease of 35% compared with a control without vitamin E). Our results suggest that UVA radiation may alter cell-presenting antigen function partly via the generation of reactive oxygen species which trigger peroxidative processes, and these data contribute to the understanding of the role of oxidative mechanisms in immune suppression induced by UVA radiation. Our in vitro model can be used to quantify UV-mediated epidermal cell damage and the degree of immune photoprotection provided by various agents.
authors: Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, Paris, France.
authors: P
authors: P
authors: Clement-Lacroix
authors: L
authors: L
authors: Michel
authors: A
authors: A
authors: Moysan
authors: P
authors: P
authors: Morliere
authors: L
authors: L
authors: Dubertret
mesh_terms: D000293:Adolescent; D000328:Adult; D017951:Antigen Presentation / Q000528:radiation effects; D018929:Cell Culture Techniques; D002455:Cell Division / Q000276:immunology; D004817:Epidermis / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D005260:Female; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects / Q000528:radiation effects*; D007801:Langerhans Cells / Q000276:immunology; D050356:Lipid Metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D010084:Oxidation-Reduction; D013858:Thiobarbiturates / Q000378:metabolism; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D013858:Thiobarbiturates; D014810:Vitamin E; C029684:thiobarbituric acid