Superoxide anion scavenging effect of coumarins.
The superoxide scavenging effects of fifteen coumarins were tested on the xanthine-xanthine oxidase-cytochrome C system. The results showed that fraxetin(10) displayed the strongest activity, and its percent inhibition at 100, 10 and 1 muM were 100, 100 and 53.13% respectively. Esculetin(4) showed the second strongest activity resulting in percent inhibition at 100 and 10 muM were 87.16 and 52.38% respectively. Both fraxetin(10) and esculetin(4) have been isolated from the plant, Fraxinus bungeana DC (Oleaceae) which has been used in folk medicine as an analgesic and anti-inflammatory medicine. It seems that two phenolic hydroxy groups in the ortho position in the molecule of coumarins play an important role in scavenging activity.
Chang
W S
WS
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
Lin
C C
CC
Chuang
S C
SC
Chiang
H C
HC
D000838:Anions / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D003574:Cytochrome c Group / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D013481:Superoxides / Q000737:chemistry*
D000838:Anions; D003374:Coumarins; D003574:Cytochrome c Group; D013481:Superoxides