title: Passage of chicken egg yolk antibody treated with hydroxypropyl methylcellulose phthalate in the gastrointestinal tract of calves.
abstract: Two types of chicken egg yolk antibody samples for oral passage trials in calves were prepared: (1) hydroxypropyl methylcellulose phthalate (HPMCP) antibody powder (HAP)--a powder produced by spray-drying a supernatant obtained after precipitation of lipids from egg yolk with HPMCP and (2) control antibody power (CAP)--a powder produced from an antibody solution with HPMCP. Antibody activity and pattern of distribution of both antibody preparations in the gastrointestinal tract of calves were compared by enzyme-linked immunosorbent assay. At 2 hr post administration, anti-K99 fimbrial antibodies from both the CAP and the HAP were detected in the abomasum of calves with titers of 1:128 and 1:256, respectively. However, at 4 hr, anti-K99 fimbrial titers of the CAP and the HAP were reduced to 1:2 and 1:64, respectively, due to digestion in the abomasum. These results indicated that the egg yolk antibody powder with HPMCP was more resistant against gastric juice in the stomach, thereby, ensuring a transfer of functional antibodies to the small intestine of calves after oral administration.
authors: Ghen Corporation, Immunology Research Institute, Gifu, Japan.
authors: Y
authors: Y
authors: Ikemori
authors: M
authors: M
authors: Ohta
authors: K
authors: K
authors: Umeda
authors: R C
authors: RC
authors: Peralta
authors: M
authors: M
authors: Kuroki
authors: H
authors: H
authors: Yokoyama
authors: Y
authors: Y
authors: Kodama
mesh_terms: D000018:Abomasum; D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000008:administration & dosage / Q000378:metabolism*; D000907:Antibodies, Bacterial / Q000378:metabolism; D002417:Cattle; D002432:Cecum; D002645:Chickens; D003106:Colon; D003126:Colostrum; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000276:immunology; D005772:Gastrointestinal Transit; D007421:Intestine, Small; D008747:Methylcellulose / Q000031:analogs & derivatives*; D012007:Rectum
chemical_list: D000906:Antibodies; D000907:Antibodies, Bacterial; D008747:Methylcellulose; C053309:hydroxypropyl methylcellulose phthalate