Regionally distinct stoichiometry for N-methyl-D-aspartate receptor domains in brain.
A stoichiometric analysis of N-methyl-D-aspartate (NMDA) receptor binding was conducted using [3H]dizocilpine, [3H]dichlorokynurenic acid and [3H]CGP39653, and membranes from various brain regions of rats. The ratio of the density of [3H]CGP39653 binding to [3H]dizocilpine binding was > 1 in frontal cortex and hippocampus, approximately 1 in striatum and spinal cord and < 1 in cerebellum. When [3H]dichlorokynurenic acid binding was compared with [3H]dizocilpine binding, the ratios were > 1 in frontal cortex, hippocampus and striatum, 3-4 in cerebellum, and approximately 2 in spinal cord. These observations suggest that a single channel complex may have more than one binding site for NMDA and/or glycine and that the stoichiometry between the binding domains of the NMDA receptor varies regionally.
Matsunaga
T
T
Neuroimaging and Drug Action Section, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Mukhin
A G
AG
London
E D
ED
D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D016291:Dizocilpine Maleate / Q000493:pharmacokinetics / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000493:pharmacokinetics / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D007736:Kynurenic Acid / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D011916:Rats, Inbred F344; D018009:Receptors, Glycine / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*
D018691:Excitatory Amino Acid Antagonists; D009419:Nerve Tissue Proteins; D018009:Receptors, Glycine; D016194:Receptors, N-Methyl-D-Aspartate; C069052:CGP 39653; D016291:Dizocilpine Maleate; D015763:2-Amino-5-phosphonovalerate; D007736:Kynurenic Acid; C066192:5,7-dichlorokynurenic acid