title: Donor-specific blood transfusion-induced tolerance in adult rats with a dominant TCR-Vbeta rearrangement in heart allografts.
abstract: Following allotransplantation, determinants encoded within the donor MHC are recognized by recipient T lymphocytes through their Ag receptor. In this study, we investigated the TCR Vbeta chain diversity of T cells infiltrating rejected and tolerated heart allografts in a model of donor-specific blood transfusion-induced tolerance in MHC-mismatched congeneic rats. The PCR-based method that we used allows the diversity of Vbeta chains at the complementarity-determining region 3 level to be analyzed quantitatively. Our results show that the Vbeta repertoire usage in graft-infiltrating T cells was characteristic and different in tolerated compared with rejected grafts, and differed in both cases from the normal distribution of the Vbeta repertoire. An expansion of lymphocytes showing a conserved Vbeta18-Dbetal-Jbeta2.7 gene rearrangement was found, from the first day after grafting onward, in graft-infiltrating cells from all tolerant animals. This clone accounted for as much as 5% of the whole Vbeta repertoire in tolerated hearts, as evidenced by RNase protection assay. In contrast, we demonstrated that, of lymphocytes infiltrating rejected grafts, those with a Vbeta18 chain were diverse, and that even though by day 5 the conserved Vbeta18-Dbeta1-Jbeta2.7 rearrangement was detectable, lymphocytes harboring this rearrangement represented less than 0.6% of the whole TCR-alphabeta+ T cell repertoire. Kinetics analysis revealed that the expansion of lymphocytes bearing this conserved rearrangement was elicited specifically by donor blood transfusion. Indeed, Vbeta18-Dbeta1-Jbeta2.7 transcripts were detected in PBL from transfused animals as early as 7 days after donor-specific blood transfusion. Finally, we provided evidence that this T cell clone belongs to the CD8+ subset. The putative role in inducing and maintaining the allograft tolerance of the CD8+ T cell clone harboring this public Vbeta18-Dbeta1-Jbeta2.7 rearrangement is discussed.
authors: Institute of Transplantation and Research on Transplantation (ITERT), National Institute of Medicine and Medical Research (Unit 437), Nantes, France.
authors: P
authors: P
authors: Douillard
authors: C
authors: C
authors: Pannetier
authors: R
authors: R
authors: Josien
authors: S
authors: S
authors: Menoret
authors: P
authors: P
authors: Kourilsky
authors: J P
authors: JP
authors: Soulillou
authors: M C
authors: MC
authors: Cuturi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001803:Blood Transfusion; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005799:Genes, Dominant; D016027:Heart Transplantation / Q000276:immunology*; D006648:Histocompatibility; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta