title: Glucocorticoid upregulation of glutamate dehydrogenase gene expression in vitro in astrocytes.
abstract: Glutamate, the major excitatory neurotransmitter, is preferentially catabolized in astrocytes by glutamate dehydrogenase (GDH). Treatment of an astrocytic cell line with hydrocortisone (10(-5) M) resulted in increased expression of GDH mRNA. Transfection of the cells with truncated parts of the GDH promoter showed that genomic responsive elements activated by hydrocortisone are localized in the -557/+1 region of the promoter. This control of GDH expression by glucocorticoids may be involved in their protective effect against glutamate excitotoxicity.
authors: INSERM U433, Faculté de Médecine A. Carrel, Lyon, France. hardin@cimacres.univ-lyon1.fr
authors: H
authors: H
authors: Hardin-Pouzet
authors: P
authors: P
authors: Giraudon
authors: M F
authors: MF
authors: Belin
authors: M
authors: M
authors: Didier-Bazes
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D005969:Glutamate Dehydrogenase / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D015854:Up-Regulation
chemical_list: D005938:Glucocorticoids; D005969:Glutamate Dehydrogenase; D006854:Hydrocortisone