HIV genome transcription induced by polyoma virus middle T antigen through both enhancer- and promoter-dependent LTR activation.
In order to understand the regulation of HIV genome transcription induced by cell stimulation through transmembrane receptors, we have transfected cells with polyoma middle T antigen (PyMT) expression vectors, thus mimicking activated receptor-dependent cell stimulation. PyMT-expressing Cos7 cells provided an environment where transcription of an HIV provirus was activated. PyMT expression induced the activity of both enhancer- and promoter-dependent HIV-LTR luciferase vectors. Induction of the HIV promoter domain depended on Sp1-binding sites and could be blocked by Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K). This indicates that PyMT-induced HIV transcription and replication are controlled by both the enhancer and promoter domains of the HIV-LTR. The latter, but not the former, was induced in a PI3K-dependent way. Thus at least 2 different transduction pathways appear to collaborate for induction of full HIV genome transcription in activated cells.
Jacqué
J M
JM
Unité d'immunologie virale, Institut Pasteur, Paris, France.
Vlach
J
J
Virelizier
J L
JL
Garcia
A
A
D000730:Androstadienes / Q000494:pharmacology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D016679:Genome, Viral; D006678:HIV / Q000235:genetics*; D016322:HIV Enhancer / Q000235:genetics; D016325:HIV Long Terminal Repeat / Q000235:genetics; D066298:In Vitro Techniques; D016328:NF-kappa B / Q000494:pharmacology; D010739:Phospholipase D / Q000037:antagonists & inhibitors; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation; D000077191:Wortmannin
D000730:Androstadienes; D000952:Antigens, Polyomavirus Transforming; D016328:NF-kappa B; D010739:Phospholipase D; D000077191:Wortmannin