DRB polymerase chain reaction fingerprinting, DQA oligotyping and mixed lymphocyte culture results in related bone marrow donors and recipients.
Donor and recipient are usually regarded as well matched for bone marrow transplantation when they are compatible in HLA class I and II antigens and in mixed lymphocyte culture (MLC). However, results of serological typing of class II antigens may be unreliable. Hence, polymerase chain reaction fingerprinting of HLA DRB (PCR FP) was introduced for the screening of related donors for 29 patients awaiting bone marrow transplantation. In addition, the sequence-specific oligonucleotide (SSO) typing of DQA alleles was performed. In 18 pairs the results of DNA analysis methods were compared with the results of MLC. 72% of pairs were HLA DQA compatible and 59% showed compatibility in PCR FP. MLC compatibility was found in 61%. A higher correlation of PCR FP and MLC results was observed.
Brojer
E
E
Institute of Hematology and Blood Transfusion, Warsaw, Poland.
Sankowska
M
M
Stańczak
J J
JJ
Gronkowska
A
A
Stepień-Sopniewska
B
B
Górski
A
A
Zupańska
B
B
D000483:Alleles; D016026:Bone Marrow Transplantation / Q000276:immunology*; D004247:DNA / Q000032:analysis / Q000235:genetics; D016172:DNA Fingerprinting; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology*; D006684:HLA-DR Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D016133:Polymerase Chain Reaction / Q000379:methods*; D014019:Tissue Donors
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D004247:DNA