[Urinary proteins (focusing on alkaline phosphatases)].
In order to gain a fuller understanding of sports-related stress markers, we studied urinary alkaline phosphatase (ALP) levels and isozyme patterns before and after strenuous exercise. ALP activity-peaks were seen 5 approximately 15 minutes or 1 hour after running. While levels of ALP of higher molecular mass were observed to increase slightly, following intense exercise, neither the intensity nor the duration of the exercise appeared to have an overly significant effect on them. After buthanol extraction, a distinct band of ALP activity was observed in the polyacrylamide gel, having the same degree of mobility as those of the renal cortex and medulla. A portion of post-exercise urinary ALP was considered to have originated from the kidney. As ALP is now understood to be one of the markers of bone metabolism, urinary ALP will most certainly come under ever more intense scrutiny, as regards its connection to sports-related stress and osteoporosis.
Sato
T
T
Division of Clinical Chemistry, Niigata Cancer Center Hospital, Japan.
Sugimoto
H
H
D000328:Adult; D000469:Alkaline Phosphatase / Q000652:urine*; D000818:Animals; D015444:Exercise / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000652:urine; D008297:Male; D011507:Proteinuria / Q000201:enzymology / Q000652:urine*
D007527:Isoenzymes; D000469:Alkaline Phosphatase