title: High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris.
abstract: The early conceptus (embryo and associated membranes) of domestic ruminats signals its presence to the maternal uterus through production of interferon-tau (IFN-tau). Production of IFN-tau ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-tau (rOvIFN-tau) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-tau, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-tau at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-tau.
authors: Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
authors: G
authors: G
authors: Van Heeke
authors: T L
authors: TL
authors: Ott
authors: A
authors: A
authors: Strauss
authors: D
authors: D
authors: Ammaturo
authors: F W
authors: FW
authors: Bazer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D003470:Culture Media; D005285:Fermentation; D007370:Interferon Type I / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010843:Pichia; D010957:Plasmids / Q000235:genetics; D011257:Pregnancy Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012636:Secretory Rate; D012756:Sheep
chemical_list: D003470:Culture Media; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau