Specific detection of anti-cell surface antibodies in herpes gestationis sera.
We examined 42 herpes gestationis sera with immunofluorescence of normal human skin sections, and found that anti-keratinocyte cell surface antibodies were detected specifically in 10 herpes gestationis sera. The diagnosis of these herpes gestationis cases was confirmed by detecting antibodies against the 180 kD bullous pemphigoid antigen with immunoblotting of its fusion protein. The results of immunoadsorption assay using baculoproteins of both pemphigus vulgaris and pemphigus foliaceus antigens indicated that the herpes gestationis sera did not recognize common pemphigus antigens. Immunoblotting of human epidermal extracts and immunofluorescence of various tissues also suggested that the sera did not recognize any other desmosomal components or paraneoplastic pemphigus antigens. The significance of this reactivity is unclear. However, because no control bullous pemphigoid sera showed this reactivity, it may suggest a different pathophysiology between herpes gestationis and bullous pemphigoid.
Hashimoto
T
T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Amagai
M
M
Murakami
H
H
Higashiyama
M
M
Hashimoto
K
K
Nishikawa
T
T
D000954:Antigens, Surface / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D001706:Biopsy; D002352:Carrier Proteins; D002462:Cell Membrane / Q000276:immunology; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000276:immunology*; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D006559:Pemphigoid Gestationis / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D012867:Skin / Q000276:immunology* / Q000473:pathology; D000097805:Collagen Type XVII
D000954:Antigens, Surface; D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human