[Overexpression of bcl-2 suppresses apoptotic cell death of the human leukemic cell line TF-1].
We analyzed the effect of overexpression of bcl-2 gene on cell cycle progression, using the growth factor dependent cell line, TF-1, derived from an erythroleukemia patient. TF-1 (bcl-2) cells, which were transfected with bcl-2 cDNA by the retrovirus vector system, survived and arrested in the G0-1 phase on GM-CSF removal. Centrifugal elutriation studies showed that G0-1-arrested subfraction of TF-1 (bcl-2) reentered the cell cycle with time delay upon GM-CSF re-addition, when compared with TF-1 (mock). A similar delay in cell cycle progression was observed during the recovery phase after 24h-exposure to staurosporine, a protein kinase C(PKC) inhibitor. These results imply functional involvement of bcl-2, both in the GM-CSF and the PKC signal transduction pathway and in G0-1 progression.
Ito
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First Department of Internal Medicine, Nagoya University School of Medicine.
Hotta
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D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D011493:Protein Kinase C; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019253:Proto-Oncogene Proteins c-bcl-2; D011493:Protein Kinase C