title: Exogenous insulin does not influence CCK- and meal-stimulated pancreatic secretion.
abstract: Although previous reports suggest interactions between the endocrine and the exocrine pancreas, insulin's effect on pancreatic exocrine function remains unclear. Chronic pancreatic fistulae were created in five dogs; these animals were studied using the euglycemic, hyperinsulinemic clamp technique. After a 30-min unstimulated period, both groups received a 60-min, 1.5 mU/kg/min insulin (clamp) or vehicle (control) infusion. Cholecystokinin (CCK) or meal stimulation was then begun. Intravenous CCK was initiated at 12.5 ng/kg/h; the CCK dose was doubled every 30 min until 100 ng/kg/h was achieved. The intraduodenal liquid test meal (1.5 kcal/ml; 15% protein, 32% fat, 53% carbohydrate) was administered at 100 ml/h. Unstimulated (0- to 30-min) serum glucose and insulin levels and pancreatic bicarbonate and protein outputs did not differ between groups. Clamp (30- to 90-min) and stimulated (90- to 210-min) insulins were significantly elevated in clamp groups (p < 0.001); glucose and bicarbonate were unchanged. Exocrine outputs during clamp periods were unaffected by insulin. Neither CCK- nor meal-stimulated pancreatic secretion (90-210 min) was influenced by insulin administration. These data suggest that hyperinsulinemia does not alter pancreatic acinar cell secretion in the intact animal.
authors: Department of Surgery, University of Cincinnati Medical Center, Ohio, USA.
authors: S M
authors: SM
authors: Berry
authors: A S
authors: AS
authors: Fink
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism*; D004285:Dogs; D005502:Food; D015309:Glucose Clamp Technique; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism*; D010185:Pancreatic Fistula / Q000503:physiopathology; D011506:Proteins / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D001786:Blood Glucose; D007328:Insulin; D011506:Proteins; D002766:Cholecystokinin