title: An immunohistochemical study of the endocrine pancreas of ducks.
abstract: Pancreatic tissue specimens of domestic ducks were fixed in PAF, paraformaldehyde and Bouin's fluid. Sections were immunohistochemically stained with antisera against insulin, glucagon, somatostatin and 5-hydroxytryptamine (5-HT). Immunoreactive cells were detected in both exocrine and endocrine pancreas. The endocrine cells were grouped into three islet types: A, B and mixed islets. A islets comprised glucagon, somatostatin and 5-HT immunoreactive cells. B islets showed insulin, somatostatin and 5-HT immuno-reactive cells. Mixed islets were composed of all four immunoreactive cytotypes. Using a double immunofluorescent technique, it was observed that 5-HT immunoreactivity was present in some of the glucagon and in some of the insulin immunoreactive cells. B islets were observed throughout the pancreas. A and mixed islets were more concentrated in the splenic lobe, and decreased in number in the other lobes. These results were compared with those obtained in other avian species.
authors: Dept. of Biological Structures, Function and Technology, University of Naples, Federico II, Italy.
authors: C
authors: C
authors: Lucini
authors: L
authors: L
authors: Castaldo
authors: O
authors: O
authors: Lai
mesh_terms: D000818:Animals; D004372:Ducks / Q000378:metabolism*; D005404:Fixatives / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D005557:Formaldehyde / Q000737:chemistry; D005934:Glucagon / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D012701:Serotonin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D005404:Fixatives; D007328:Insulin; D011108:Polymers; D005557:Formaldehyde; D012701:Serotonin; D013004:Somatostatin; D005934:Glucagon; C003043:paraform