sigma54-dependent transcription of the Pseudomonas putida xylS operon is influenced by the IIANtr protein of the phosphotransferase system in Escherichia coli.
IIANtr, encoded within the rpoN operons of many Gram-negative bacteria, is a homologue of a class of phosphoryl transfer proteins of the phosphoenolpyruvate: sugar phosphotransferase system. We have used a xylS operon-lacZ fusion from the TOL plasmid of Pseudomonas putida to show that IIANtr influences sigma 54-dependent transcription when the xylS operon is expressed in Escherichia coli. Loss of IIANtr influences, but does not abolish cyclic AMP-independent carbon catabolite repression.
Du
Y
Y
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
Holtel
A
A
Reizer
J
J
Saier
M H
MH
D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D066298:In Vitro Techniques; D009876:Operon / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D012808:Sigma Factor; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System