Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene.
The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc4l YJO to grow on mannitol with substantial NAD-dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.
Perfect
J R
JR
Duke University Medical Center, Durham, North Carolina, USA.
Rude
T H
TH
Wong
B
B
Flynn
T
T
Chaturvedi
V
V
Niehaus
W
W
D000449:Aldehyde Reductase / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003455:Cryptococcus neoformans / Q000235:genetics*; D004271:DNA, Fungal; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D008354:Mannitol Dehydrogenases / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; C101654:Mtl-1 protein, Cryptococcus neoformans; D014157:Transcription Factors; D008354:Mannitol Dehydrogenases; D000449:Aldehyde Reductase; C023142:polyol dehydrogenase (NADP)