title: Mitochondrial electron transport chain activities and DNA deletions in regions of the rat brain.
abstract: Deletions in human mitochondrial DNA cause various mitochondrial myopathies and increase markedly with age in highly oxidative tissues, but exhibit a differential distribution in the brain. In order to determine whether a similar pattern occurs in rat brain the levels of a 4.8 kb deletion and electron transport complex activities were measured in the striatum, hippocampus, cerebellum, and cerebral cortex of young adult and senescent male Wistar rats. Deletion-containing mtDNA was present at relatively similar levels (0.0003%) in all regions in 6 mo rats, but increased 25-, 7-, 3-, and 2-fold in the striatum, hippocampus, cerebral cortex, and cerebellum, respectively, of 22-23 mo old rats. To assess the relationship between fractional occurrence of a deletion and oxidative phosphorylation capacity, the activities of mitochondrial respiratory chain complexes I, III, IV and V, the mitochondrial ATP-ase, each of which contains subunits encoded in mtDNA, were determined in homogenates. No age-related decrements in activity were observed in any of the brain regions. Thus, while mtDNA deletions increase with age and to a large extent mirror the pattern observed in the human brain, they appear to have no effect on capacity for oxidative phosphorylation of distinct brain regions. Any reductions in capacity that may be present are likely to occur only at the level of individual cells.
authors: Laboratory of Biological Sciences, National Institute on Aging, Baltimore, MD 21224, USA.
authors: C R
authors: CR
authors: Filburn
authors: W
authors: W
authors: Edris
authors: M
authors: M
authors: Tamatani
authors: B
authors: B
authors: Hogue
authors: I
authors: I
authors: Kudryashova
authors: R G
authors: RG
authors: Hansford
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004579:Electron Transport; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D017384:Sequence Deletion; D014018:Tissue Distribution
chemical_list: D004272:DNA, Mitochondrial