title: High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae.
abstract: Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliae using benomyl resistance as a selectable marker. Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants micrograms-1 of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants micrograms-1 of DNA and 67% by electroporation; and 32 to 201 transformants micrograms-1 of DNA and 90% by biolistic. We demonstrate by PCR that 60% of the transformants were generated by gene conversion.
authors: Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors: M R
authors: MR
authors: Bogo
authors: M H
authors: MH
authors: Vainstein
authors: F J
authors: FJ
authors: Aragão
authors: E
authors: E
authors: Rech
authors: A
authors: A
authors: Schrank
mesh_terms: D000818:Animals; D001483:Base Sequence; D001542:Benomyl / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018274:Electroporation; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005785:Gene Conversion; D005819:Genetic Markers; D003904:Mitosporic Fungi / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D011092:Polyethylene Glycols; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005659:Fungicides, Industrial; D005819:Genetic Markers; D011092:Polyethylene Glycols; D001542:Benomyl