Affinity maturation of proteins displayed on surface of M13 bacteriophage as major coat protein fusions.
This chapter described the preparation and fractionation of libraries of M13 phage displaying proteins as fusions to the major coat protein. High titer (10(13) pfu/ml) phage libraries can readily be generated using a single vector and the level of display surpasses that of gene III fusion phage. Since the synthetic VIII fusion gene can be customized, this system should provide the flexibility required to construct phage libraries displaying a variety of different peptides and proteins and to select variants possessing the highest affinity for target molecules of a diverse chemical nature.
Roberts
B L
BL
Genzyme Corporation, Framingham, Massachusetts 01701, USA.
Markland
W
W
Ladner
R C
RC
D017104:Bacteriophage M13 / Q000235:genetics*; D001483:Base Sequence; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism*; D002213:Capsid / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009842:Oligopeptides / Q000235:genetics*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins