[The value of polymerase chain reaction on Epstein-Barr virus subtypes in the differentiation of benign and malignant nasopharyngeal biopsies].
Polymerase chain reaction (PCR) of Epstein-Barr virus (EBV) EBNA-2 type A (PCR-A) along with dot blot hybridization using EBV DNA Bam-W and EBNA-2 type A and B fragment as the probes (DBH-W, -A, and -B) was performed in the nasopharyngeal biopsies. The positive rates of PCR-A, DBH-W, -A and -B were 83.9%, 80.6%, 74.2% and 0.0% in 31 cases of nasopharyngeal, respectively, and 12.0%, 40.0%, 92.0% and 0.0% in 25 cases of chronic nasopharyngitis (NP), respectively. There were significant differences between NPC and NP or non-NPC (P < = 0.0001) detected by PCR-A. The results indicated that positive PCR-A would be valuable for early diagnosis of nasopharyngeal carcinoma. The reasons which might make those differences were discussed.
Sun
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N
Guangdong Medical College, Zhanjiang.
Chen
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X
D000956:Antigens, Viral / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D003937:Diagnosis, Differential; D019309:Epstein-Barr Virus Nuclear Antigens; D006566:Herpesviridae Infections; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000276:immunology / Q000821:virology*; D009304:Nasopharyngitis / Q000276:immunology / Q000821:virology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections
D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens