Calbindin D28K forms a Ca(2+)-dissociable complex with mellitin in vitro.
Calbindin D28K (CB), a cytosolic calcium binding protein (CBP), forms a macromolecular complex with the polypeptide mellitin (ME) the absence of calcium, which can be reversibly dissociated by the addition of Ca2+. The molar ratio of CB:ME constituted in this complex is 1:4, suggesting that CB interacts with the tetrameric form of ME. Like free tetrameric ME, the CB:ME complex does not migrate into 15% non-denaturing polyacrylamide electrophoretic gels, although both constituents migrate normally after irreversible complex denaturation by heating in sodium dodecyl sulphate (SDS). The interaction of these two proteins can be distinguished from the association of calmodulin (CM) with ME, which forms a reversibly dissociable, equimolar complex in the presence of Ca2+ and a stable non-migrating complex (molar ratio = 1:12) in its absence. Thus, CB and CM appear to bind ME under different Ca2+ regulatory control, suggesting possible roles for CB as a Ca(2+)-dependent regulatory binding protein.
La Bella
V
V
Department of Neurology, Baylor College of Medicine, Houston, Texas, USA.
Ho
B K
BK
Appel
S H
SH
Smith
R G
RG
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000378:metabolism; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008555:Melitten / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000302:isolation & purification / Q000378:metabolism*
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D002147:Calmodulin; D064030:S100 Calcium Binding Protein G; D008555:Melitten; D002118:Calcium