title: Protective effect of the superoxide scavenger EUK8 against ultrastructural alterations induced by ischemia and reperfusion in isolated rat hearts.
abstract: We have recently shown that iron overload may increase the susceptibility of isolated rat hearts to ischemia and reperfusion, an effect that could be related to an iron-dependent activation of the Fenton reaction, thus producing cytotoxic hydroxyl radicals. The purpose of our study was to investigate the influence of EUK8, a new catalytic scavenger of superoxide anions that is similar to superoxide dismutase, on cardiac ultrastructural alterations associated with reperfusion. Experiments were carried out ex vivo in iron-overloaded rat hearts perfused at a constant flow rate (11 ml/min) with (EUK8) or without (control) EUK8 (50 mumol/L) throughout the perfusion. Hearts were submitted to 15 min global normothermic no-flow ischemia and then reperfused for 15 min before examination. For each heart, six micrographs at a magnification of x 11,300 were used to quantitate ultrastructural alterations. In our experimental model, EUK8 prevented some of the ultrastructural modifications associated with ischemia and reperfusion in iron-overloaded rat hearts. This beneficial effect could be related to the limitation of reperfusion-induced oxygen free radical production under the effect of EUK8.
authors: Groupe de Physiopathologie Cellulaire Cardiaque, Université Joseph Fourier, Grenoble, France.
authors: S
authors: S
authors: Pucheu
authors: F
authors: F
authors: Boucher
authors: B
authors: B
authors: Malfroy
authors: J
authors: J
authors: De Leiris
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D016166:Free Radical Scavengers; D008928:Mitochondria / Q000648:ultrastructure; D017202:Myocardial Ischemia / Q000175:diagnosis* / Q000473:pathology / Q000628:therapy; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000648:ultrastructure*; D009210:Myofibrils / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012518:Sarcomeres / Q000648:ultrastructure; D013481:Superoxides / Q000378:metabolism*
chemical_list: D016166:Free Radical Scavengers; D013481:Superoxides