Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida.
Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.
Conrad
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Oklahoma State University College of Osteopathic Medicine, Department of Biochemistry and Microbiology, Tulsa 74107-1898, USA.
Galanos
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Champlin
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D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D001717:Birds; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D005612:Freeze Drying; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016979:Pasteurella multocida / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification
D000606:Amino Sugars; D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides