Liquid chromatographic determination of carotenoids in human serum using an engineered C30 and a C18 stationary phase.
A C30 stationary phase was specifically engineered for carotenoid separations, and carotenoid measurements using this column are compared with those obtained using a somewhat more conventional C18 column. Both methods were used to contribute measurements for the certification of carotenoids in Standard Reference Material 968b, Fat-Soluble Vitamins and Cholesterol in Human Serum. Analytes were extracted from the serum into hexane. Measurements on the C18 column were made using a gradient of acetonitrile, methanol, and ethyl acetate, which is described in detail elsewhere. Measurements on the C30 column were made using a gradient of water, methanol, and methyl tert.-butyl ether.
Sharpless
K E
KE
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Thomas
J B
JB
Sander
L C
LC
Wise
S A
SA
D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D065366:Cryptoxanthins; D006801:Humans; D014975:Lutein / Q000097:blood; D000077276:Lycopene; D011786:Quality Control; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000097:blood
D065366:Cryptoxanthins; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene; D000077276:Lycopene; D014975:Lutein