Prevalence of anti-R-13 antibodies in human Trypanosoma cruzi infection.
Infection with Trypanosoma cruzi develops in three phases: acute, indeterminate or asymptomatic, and chronic phase (with cardiac or digestive manifestations). Moreover, transmission may occur from infected mothers to newborn, the so-called congenital form. In the present study, humoral responses against T. cruzi total extract and against the 13 amino acid peptide named R-13 derived from the parasite ribosomal P protein, previously described as a possible marker of chronic Chagas heart disease, were determined in chagasic patients and in blood bank donors from endemic areas. While in sera from acute phase, only IgM anti-T.cruzi response was observed, both IgM and IgG anti-T. cruzi antibodies were detected in sera from congenitally infected newborns. The percentage of positive response in sera from blood bank donors was relatively high in endemic regions. Antibodies against the R-13 peptide were present in a large proportion of cardiac chagasic patients but were totally lacking in patients with digestive form of Chagas' disease. Furthermore, anti-R-13 positive responses were detected in congenitally infected newborns.
Aznar
C
C
Centre de Biologie Medicale Specialisee, Institut Pasteur, Paris, France.
Lopez-Bergami
P
P
Brandariz
S
S
Mariette
C
C
Liegeard
P
P
Alves
M D
MD
Barreiro
E L
EL
Carrasco
R
R
Lafon
S
S
Kaplan
D
D
D000208:Acute Disease; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D001118:Argentina; D001483:Base Sequence; D001782:Blood Donors; D001938:Brazil; D002598:Chagas Cardiomyopathy / Q000175:diagnosis / Q000276:immunology; D014355:Chagas Disease / Q000151:congenital / Q000453:epidemiology / Q000276:immunology*; D002908:Chronic Disease; D003937:Diagnosis, Differential; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D012269:Ribosomal Proteins / Q000276:immunology*; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan; D007132:Immunoglobulin Isotypes; C064601:L12E protein, Trypanosoma cruzi; D015800:Protozoan Proteins; D012269:Ribosomal Proteins