Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea.
There has been recent increasing interest in the involvement of superoxide radicals (O2-) and their scavenging enzymes, the superoxide dismutases, in the patho-physiology of certain diseases. Since mitochondria are significant intracellular sources of O2- and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2- scavenging enzyme may provide important insight into the pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2- scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2- was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD immunohistochemically from ethylenediamine tetra-acetic acid-treated materials without requiring an antigen retrieval procedure.
Lai
M T
MT
Department of Otolaryngology, Okayama University Medical School, Japan.
Ohmichi
T
T
Egusa
K
K
Okada
S
S
Masuda
Y
Y
D000818:Animals; D003051:Cochlea / Q000201:enzymology* / Q000473:pathology; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000473:pathology; D007758:Ear, Inner / Q000201:enzymology / Q000473:pathology; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012016:Reference Values; D013482:Superoxide Dismutase / Q000378:metabolism*
D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase