[Cell culture and freeze-preservation of the human laryngeal epithelial cells].
The laryngeal epithelial cells for culture came from healthy fetuses of induced labor of middle and late period. Cells were cultured under 37 degrees C, 5% carbon dioxide and saturated humidity. The results showed that in the primary generation was cultured for 7 days, the aggregens could be seen by the naked eye. In 10-14 days, the cell layers formed and gradually covered the whole bottom of the flasks. The second generation grew faster than the primary one. Twenty flasks of the cultured cells were freeze-preserved for 14 to 28 days, of which 5 flasks (25%) were resuscitated successfully. By means of the cell culture, some characteristics of the laryngeal epithelial cells can be studied.
Li
D
D
Tangdu Hospital, Fourth Medical University, Xian.
Chen
W
W
Zhou
H
H
D002478:Cells, Cultured; D015925:Cryopreservation; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005333:Fetus; D006801:Humans; D007830:Larynx / Q000166:cytology*