title: Antibody-mediated lysis of the bovine subcommissural organ maintained in culture.
abstract: The subcommissural organ (SCO) is a brain gland that secretes glycoproteins into the cerebrospinal fluid (CSF). It is an ancient and conserved secretory structure of the brain, developing very early in ontogeny. However, the function of the SCO is unknown. The secretory cells of the SCO are arranged into a single or double, irregularly shaped layer located at the interface of the CSF and nervous tissue. This has prevented its selective surgical destruction. The present investigation was designed to destroy the secretory cells of 30-day-old explants of bovine SCO by use of an immunological approach. A membrane preparation enriched with plasma membrane of the secretory cells of the bovine SCO was obtained. This preparation was further processed to separate the structural proteins. A similar procedure was applied to obtain a fraction of integral proteins of the plasma membrane of a nonsecretory ciliated ependyma. Antisera were prepared against both preparations of integral proteins. The antiserum against the fraction obtained from the SCO cells immunostained the plasma membrane of the bovine SCO cells and in immunoblot it reacted with several proteins of the membrane preparation from SCO cells. When added to the culture medium this antibody bound to the apical plasma membrane of the secretory ependyma of the bovine SCO kept in culture; it caused the lysis of these cells when used together with complement. None of these properties were displayed by the antiserum raised against the integral proteins of the plasma membrane of the ciliated ependyma. This antiserum, however, immunostained the bovine ciliated ependyma neighboring the SCO. These results indicate that immunological surgery of the SCO in living animals may be possible to achieve.
authors: Instituto de Histología y Patolog , Universidad Austral de Chile, Valdivia, Chile.
authors: M
authors: M
authors: Cifuentes
authors: E M
authors: EM
authors: Rodríguez
authors: S
authors: S
authors: Hernández
authors: J
authors: J
authors: Pérez
authors: B
authors: B
authors: Peruzzo
authors: Pérez-Fígares
authors: P
authors: P
authors: Fernández-Llebrez
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity; D015153:Blotting, Western; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000737:chemistry / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004805:Ependyma / Q000166:cytology; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis; D008854:Microscopy, Electron; D009490:Neurosecretory Systems / Q000166:cytology; D011817:Rabbits; D051381:Rats; D013351:Subcommissural Organ / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D008565:Membrane Proteins