Effects of ofloxacin administration on the reliability of urine glucose testing.
OBJECTIVE
To study the effects of ofloxacin on the reliability of urine glucose testing.


METHODS
Open-label, nonrandomized.


METHODS
A university-affiliated tertiary care hospital, ambulatory clinic.


METHODS
Ten healthy volunteers (8 men and 2 women) aged 22-39 years.


METHODS
Phase I (in vitro) involved the addition of selected amounts of ofloxacin to a set of standard 50-mL urine samples prepared to simulate glycosuria. Phase II (in vivo) involved the oral administration of ofloxacin 400 mg to 10 subjects. Urine was collected: (1) immediately predose, (2) pooled 0-4 hours postdose, and (3) pooled 4-8 hours postdose. Known glucose concentrations were then added to these samples. Clinitest and Diastix tests were performed on all samples. The accuracy of these tests in determining glucose concentrations was compared among urine samples taken before and after ofloxacin dosing.


RESULTS
None of the ofloxacin concentrations in phase I (0, 25, 50, 100, 200, 400, and 800 micrograms/mL) influenced these testing methods at the urine glucose concentrations of 0.0%, 0.5%, 1%, and 2%. Likewise, the accuracy of these two tests was unaffected by ofloxacin administration in phase II.


CONCLUSIONS
In single-dose administration, ofloxacin does not interfere with Clinitest or Diastix for determining urine glucose concentrations.
Tsai
T M
TM
Massachusetts College of Pharmacy and Allied Health Sciences, Boston 02115, USA.
Shea
B F
BF
Souney
P F
PF
Volinsky
F G
FG
Scavone
J M
JM
O'Neill
S
S
D000328:Adult; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006029:Glycosuria / Q000139:chemically induced* / Q000175:diagnosis*; D006801:Humans; D008297:Male; D015242:Ofloxacin / Q000494:pharmacology*; D015203:Reproducibility of Results
D015242:Ofloxacin