Rare association of human herpesvirus 6 DNA with human papillomavirus DNA in cervical smears of women with normal and abnormal cytologies.
We investigated by nested PCR the possible association of human herpesvirus 6 (HHV-6) and human papillomavirus (HPV) genomes in the cervixes of 109 women with normal and abnormal cytological smears. HPV DNA was detected in 8.33% of 24 women with normal cytologies and in 41.1% of 85 women with abnormal cytologies; the proportion of HPV DNA was directly related to the severity of the lesions. HHV-6 DNA was found in only one patient, who had a cytological pattern of koilocytosis. The HHV-6 genome was classified by restriction enzyme analysis as variant B. The study indicates that detection of the HHV-6 genome in the cervixes of women with a wide spectrum of gynecological complaints is a rare event and rules out the possible association between HHV-6 and HPV genomes in cervical cancer lesions.
Romano
N
N
Dipartimento di Igiene e Microbiologia G. D'Alessandro, Università degli Studi di Palermo, Italy.
Romano
F M
FM
Viviano
E
E
Vitale
F
F
Villafrate
M R
MR
Perna
A M
AM
Bonura
F
F
Guttadauro
R
R
D000328:Adult; D002584:Cervix Uteri / Q000166:cytology / Q000473:pathology / Q000821:virology*; D003043:Cocarcinogenesis; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000821:virology
D004279:DNA, Viral