Photodynamic treatment of subcutaneous tumors in mice with photofrin II and Argon ion laser.
OBJECTIVE
To investigate the combined effects of photofrin (Pf) II and laser irradiation on mice bearing subcutaneous tumors.


METHODS
Ehrlish ascites tumor cells (1 x 10(7)) were implanted subcutaneously into the breast area of ICR mice. The tumors, when grew up to 0.55 +/- 0.15cm3, was administered of laser irradiation (514.5nm, 112.5mW/cm2 for 11.12 min with a total energy 75j/cm2) after intraperitoneal injection of Pf II (5, 7.5, 10 and 15mg/kg body weight) once a day for three successive days.


RESULTS
The mortality rate (MR120) in the tumor control was 93.7% and the mean survival time (MST120) was 46.6 days. The best therapeutic effect (50% mortality rate, 53.8% tumor control rate and mean survival time 86 days) was observed in the group treated with 7.5mg of Pf II per kg of body weight and total irradiation energy of 75J/cm2 (112.5mW/cm2 for 11.12 min) with 514.5nm argon ion laser light.


CONCLUSIONS
The effect on the survival rate of tumor cells and synthetic rates of DNA, RNA and protein were obviously inhibited, as the dose of pf II and laser irradiation increased.
You
J S
JS
Institute of Radiation Biology, National Tsing Hua University, Taiwan.
Hau
D M
DM
Chen
G T
GT
Huang
H F
HF
Chen
W C
WC
Lin
I H
IH
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D005260:Female; D017324:Hematoporphyrin Derivative / Q000627:therapeutic use*; D006414:Hematoporphyrin Photoradiation; D053685:Laser Therapy; D051379:Mice; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D017319:Photosensitizing Agents / Q000627:therapeutic use; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative