Occurrence of [copper, zinc]-cofactored superoxide dismutase in Pasteurella haemolytica and its serotype distribution.
Fifty-two ovine strains of Pasteurella haemolytica and P. trehalosi representing serotypes 1-16 were examined for the presence of [copper, zinc]superoxide dismutase DNA sequences. This was done using a combination of polymerase chain reaction with degenerate primers based on the sequence of the [Cu,Zn]superoxide dismutase gene (sodC) in related species and Southern hybridization using a fragment of sodC from P. haemolytica A2 serotype as a probe. Both detection methods identified a fragment of the sodC gene in 9/9 strains of P. haemolytica serotype 2 examined and in 5/8 strains of serotype 7. No evidence of this gene was found in any other serotype of P. haemolytica or in any P. trehalosi serotype. Comparison of DNA sequence showed near identity between sodC from the A2 and A7 serotypes of P. haemolytica and substantial similarity (70%) to sodC previously sequenced in P. multocida, Haemophilus parainfluenzae and H. influenzae. Analysis by gel electrophoresis of the superoxide dismutase activity present in cell lysates showed that one or more superoxide dismutase is present in all serotypes. However, cyanide-inhibitable activity, corresponding to [Cu,Zn]superoxide dismutase, was detected only in those strains of serotypes A2 and A7 which showed evidence of the sodC gene fragment.
Lainson
F A
FA
Moredun Research Institute, Edinburgh, UK.
Thomson
N
N
Rowe
H A
HA
Langford
P R
PR
Aitchison
K D
KD
Donachie
W
W
Kroll
J S
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D006190:Haemophilus / Q000201:enzymology / Q000235:genetics; D006193:Haemophilus influenzae / Q000201:enzymology / Q000235:genetics; D016978:Mannheimia haemolytica / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010325:Pasteurella / Q000201:enzymology / Q000235:genetics; D016979:Pasteurella multocida / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D012756:Sheep; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D013482:Superoxide Dismutase