Three-dimensional reconstruction of the cochlea from two-dimensional images of optical sections.
This paper describes a methodology for three-dimensional (3D) computer-aided reconstruction of the guinea pig cochlea using orthogonal-plane fluorescence optical sectioning. Specimens are sectioned optically, allowing them to remain intact during observation. Equations to correct the data for specimen translation and rotation are developed and 3D reconstructions of the scala tympani, round window membrane, and cochlear aqueduct are presented. The error associated with the reconstruction is estimated to be < 19 microns.
Voie
A H
AH
Center for Bioengineering, University of Washington, Seattle 98195, USA.
Spelman
F A
FA
D000818:Animals; D003051:Cochlea / Q000033:anatomy & histology*; D003052:Cochlear Aqueduct / Q000033:anatomy & histology; D016288:Computer Terminals; D017076:Computer-Aided Design; D006168:Guinea Pigs; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D016247:Information Storage and Retrieval; D008856:Microscopy, Fluorescence; D008867:Microtomy; D012399:Rotation; D012405:Round Window, Ear / Q000033:anatomy & histology; D012533:Scala Tympani / Q000033:anatomy & histology