[Simplified CD4+ T-lymphocyte count in patients with HIV/AIDS in Mexico].
OBJECTIVE
To study the correlation between ALC and CD4 cell counts and to find alternative ways of counting CD4+ T-lymphocytes.


METHODS
The double positivity for CD3/CD4 antibodies was measured in 175 consecutive HIV-positive patients using flow cytometry; in these cases a difference was made between counting cells that were positive for both antibodies vs those that were positive only to CD4. ALC and CD4 counts were also compared among 500 subjects infected with HIV, using linear regression analysis and comparing the number of dissimilar cases for counts below 200 cells/microliters and ALC counts lower than 1 500 and 2000 cells/microliters.


RESULTS
In the 175 cases measured by the CD3/CD4 antibody combination the mean percent was 13.8% and for total CD4 cells 14.2% (p = NS); the mean difference was 0.4% and the median 0.2%. For the 500 patients the mean ALC was 1 700 cells/microliters and the mean CD4 count was 219 cells/microliters; the correlation coefficient was 0.59.


CONCLUSIONS
These data suggest a poor correlation of ALC and CD4 cell counts, thus it is impossible to predict CD4 on the basis of ALC. This is the reason why it is necessary to measure CD4 cells separately. The difference between measuring double positive CD3/CD4 cells vs only CD4 positive cells was not significant. Our data suggest that the use of a single CD4 antibody may cut costs and still produce clinically useful information.
Guarner
J
J
División de Servicios Auxiliares de Diagnóstico y Tratamiento, Instituto Nacional de Cancerología (INC), México.
Sánchez-Mejorada-Fernández
G
G
del Río-Chiriboga
C
C
Mohar
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D016014:Linear Models; D018655:Lymphocyte Count; D012680:Sensitivity and Specificity
Simplificación en el conteo de linfocitos T CD4 positivos en personas con infección por VIH/SIDA en México.