title: [Effects of chronic electrical stimulation on cochlear nuclear neuron size in deaf kittens].
abstract: It is now well recognized that normal afferent innervation is necessary for development of the auditory pathway. This study investigated the effect of chronic electrical stimulation of the auditory nerve on the size of cochlear nucleus somata of neonatally deafened kittens. Four kittens were deafened by using kanamycin and ethacrynic acid at 10 days of age and implanted at various ages with an active intracochlear implant in the left side and a dummy implant in the right side, and chronically electrically stimulated from ages 98-142 days for approximately 1000 hours at twice the EABR threshold. After chronic stimulation or after an equivalent period in the controls, the animals were injected with 2-deoxyglucose (2DG) and stimulated electrically for 45 minutes, then processed for autoradiography. Then the sections were stained for Nissle substance, and the cross-sectional areas (CSSA) of approximately 33,000 neuron somata in the cochlear nuclei were measured with an image-analysis system. Soma sizes in regions with 2DG uptake were compared with those without the uptake. The CSSA of 2DG-labeled regions was usually significantly larger than that of the unlabeled regions, but there were no significant right-left differences. Thus, chronic electrical stimulation of a restricted sector of the cochlea is correlated with a larger soma size in the corresponding region of the cochlear nucleus.
authors: Department of Otolaryngology, Tokyo Medical College.
authors: A
authors: A
authors: Kawano
authors: E
authors: E
authors: Hakuhisa
authors: S
authors: S
authors: Funasaka
mesh_terms: D000818:Animals; D002415:Cats; D003054:Cochlear Implants; D017626:Cochlear Nucleus / Q000166:cytology*; D003638:Deafness / Q000473:pathology; D004558:Electric Stimulation