Genomic footprinting of HTLV type I and HIV type 1 in human T cell lines.
Genomic footprinting of integrated HTLV-I and HIV-1 confirmed many aspects of retroviral transcriptional regulation deduced from previous studies. However, many notable differences were seen. HTLV-I genomic protein-binding patterns corresponded more closely to elements defined by transient transfection expression studies than to those mapped by in vitro protein-binding studies. HIV-1 genomic footprinting showed activation-related binding to adjacent NF-KB/SP1 sites and a large (90 bp) region transversing the R/U5 boundary, but minimal protein binding to NFAT, NRE, LBP-1, and CTF/NF1 sites relative to previous in vitro footprinting studies.
Brown
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Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
Xu
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Nerenberg
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D001483:Base Sequence; D002460:Cell Line; D018983:DNA Footprinting; D015497:HIV-1 / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011533:Proviruses / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000821:virology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D016662:Virus Integration
D014157:Transcription Factors; D013755:Tetradecanoylphorbol Acetate