[Establishment and characterization of a model of highly metastatic human ovarian cancer transplanted subcutaneously into nude mice].
A model of highly metastatic human ovarian cancer transplanted subcutaneously into nude mice was established (NMSO). Although the NMSO transplanted tumors were passaged 23 times, they retained their highly metastatic behavior. A total of 57 adult Balc/c nude mice (8-14 weeks old) were inoculated (SC) with tumor, the transplantations were 100% successful and the average survival period was 159.9 days. 47 mice were dissected and 42 mice were found to have metastatic tumors. The earliest appearance of metastasis was 56 dys. 18 male nude mice all had metastasis. Histology and ultrastructure showed that the metastatic tumors retained their malignant features and secreting function of the original poorly differentiated human ovarian serous papillary cystadenocarcinoma. FCM analysis gave a 1.4 DNA index and the chromosome mode number was 54 (hyper-diploid), exhibiting the features of human carcinoma. The detection of correlative label material showed that most cancer cells were ER and PR positive. The use of NMSO model may lead to better understanding of the mechanism of metastasis and help search for anti-metastatic agents.
Xu
S
S
Zhejiang Cancer Research Institute, Hangzhou.
Mou
H
H
Quian
L
L
D000818:Animals; D018283:Cystadenocarcinoma, Papillary / Q000556:secondary*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*