title: Use of DNA restriction fragment typing in the differentiation of Mycobacterium tuberculosis complex isolates from animals and humans in Burundi.
abstract: METHODS
  Bubanza Hospital and Veterinary Laboratory in Bujumbura, Burundi.
  
  
  OBJECTIVE
  To monitor the rate of Mycobacterium bovis infections among tuberculosis (TB) patients and among slaughtered cattle, and to analyse the polymorphism among deoxyribonucleic acid (DNA) fingerprints of the M. tuberculosis complex isolates.
  
  
  METHODS
  135 lymph node biopsies and 35 sputum specimens from human patients, together with specimens from 46 healthy animals and 36 animals suspected for bovine tuberculosis (BTB), were cultured. Isolates were identified phenotypically and DNA fingerprints were obtained by IS6110 based restriction fragment length polymorphism.
  
  
  RESULTS
  119 M. tuberculosis complex isolates were obtained from 170 human specimens. M. bovis was not identified in any human sample. One out of 46 healthy animals and 14 out of 36 BTB suspected animals yielded M. bovis isolates. DNA fingerprinting revealed four to eight copies of IS6110 for all M. bovis isolates with some degree of polymorphism, and some clustering for human TB isolates. No relationship was observed between human and bovine isolates.
  
  
  CONCLUSIONS
  At present M. bovis seems to play a minor role in human TB in Burundi, despite the high prevalence of both human immunodeficiency virus infection in humans and M. bovis in cattle. DNA fingerprinting is able to differentiate between bovine isolates.
authors: Instituut voor Tropische Geneeskunde, Antwerpen, Belgium.
authors: L
authors: L
authors: Rigouts
authors: B
authors: B
authors: Maregeya
authors: H
authors: H
authors: Traore
authors: J P
authors: JP
authors: Collart
authors: K
authors: K
authors: Fissette
authors: F
authors: F
authors: Portaels
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015373:Bacterial Typing Techniques; D002063:Burundi; D002417:Cattle; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000145:classification / Q000302:isolation & purification; D009163:Mycobacterium bovis / Q000145:classification / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000382:microbiology*; D014380:Tuberculosis, Bovine / Q000382:microbiology*