title: Genotoxicity of wood dust in a human embryonic lung cell line.
abstract: Wood dust exposure has been found to be an occupational hazard, being linked to an enhanced incidence of various neoplasias. Here we performed an experiment to evaluate the ability of solvent extracts of natural woods to induce chromosome aberrations in respiratory cells in culture. Human embryonic lung cells, MRC-5, grown in Dulbecco's medium were exposed to various concentrations of the dust extracts of pesticide-free (untreated) beech, oak and pine woods. Three concentrations per extract with and without metabolic activation (S9) and 100 metaphase cells per dose were examined for possible structural aberrations. Although no dose-dependent activity could be found with any extract in the presence of S9, most aberrations observed were of the chromatid type caused by oak wood. Dose-dependent chromosomal breaks caused by oak and chromatid breaks caused by both beech and oak were observed in the absence of S9. These data might support the early hypothesis that hard wood dust per se contains some in vivo genotoxic and thus possibly carcinogenic components.
authors: Institute of Hygiene and Occupational Medicine, University Medical Centre, Essen, Germany.
authors: Z C
authors: ZC
authors: Zhou
authors: K H
authors: KH
authors: Norpoth
authors: E
authors: E
authors: Nelson
mesh_terms: D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D002869:Chromosome Aberrations; D004391:Dust / Q000009:adverse effects*; D004622:Embryo, Mammalian; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D014197:Trees; D014934:Wood
chemical_list: D002273:Carcinogens; D004391:Dust