title: Membrane-based, dry-reagent prothrombin time tests.
abstract: The authors describe a prototype membrane-based, dry-reagent prothrombin time assay for whole blood. This system uses an asymmetric polysulfone membrane to separate plasma from red blood cells, and works with samples as small as 10 microliters. The membrane contains calcium and thromboplastin, and permits the reactions of the complete extrinsic pathway to occur with minimal distortion from membrane surface interactions. Thrombin generation is monitored optically using a rhodamine-110-based fluorescent thrombin substrate. Fluorescence kinetics are analyzed to produce a prothrombin-time--equivalent parameter that can be converted to an international normalized ratio (INR) value. The system provides results that correlate well with conventional liquid phase prothrombin time assays (R2 = 0.96).
authors: Avocet Medical, Inc., Los Gatos, CA 95032, USA.
authors: S E
authors: SE
authors: Zweig
authors: B G
authors: BG
authors: Meyer
authors: S
authors: S
authors: Sharma
authors: C
authors: C
authors: Min
authors: J M
authors: JM
authors: Krakower
authors: S B
authors: SB
authors: Shohet
mesh_terms: D000925:Anticoagulants / Q000627:therapeutic use; D001672:Biocompatible Materials / Q000737:chemistry; D001777:Blood Coagulation / Q000187:drug effects; D002118:Calcium; D004912:Erythrocytes; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents / Q000008:administration & dosage*; D008567:Membranes, Artificial; D010949:Plasma; D011108:Polymers / Q000737:chemistry; D011517:Prothrombin Time; D012235:Rhodamines; D013450:Sulfones / Q000737:chemistry; D013917:Thrombin / Q000096:biosynthesis; D013925:Thromboplastin; D014859:Warfarin / Q000627:therapeutic use
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011108:Polymers; D012235:Rhodamines; D013450:Sulfones; C049025:rhodamine 110; C017662:polysulfone P 1700; D014859:Warfarin; D013925:Thromboplastin; D013917:Thrombin; D002118:Calcium