Development of a simple, rapid and accurate in vitro whole blood technique for the detection and semi-quantification of FIV cellular viremia.
A new, simple, rapid and accurate culture technique is described for a semi-quantitative analysis of cellular viremia in FIV-infected cats. This assay can be carried out with small amounts of whole blood, and is based on the detection of FIV core gag antigen, which is released in culture supernatants. The amount of core antigen produced is measured with an enzyme-linked immunoassay using specific monoclonal antibodies. This whole blood technique (WB method) was compared with a culture method using isolated peripheral blood mononuclear cells (PBMC method). FIV could be detected in whole blood of all experimentally infected cats, but not from uninfected cats. This assay offers a number of advantages (small blood samples required, no leukocyte separation and lymphocyte purification procedures) and its reproducibility is very good. It provides a convenient in vitro cellular assay for viral semi-quantitation, well adapted for monitoring efficacy of prototype FIV vaccines or experimental antiviral drugs. Also, it could facilitate the study of the pathogenesis of FIV-related progressive immunodepression. Finally, it offers an alternative to serological techniques for diagnostic purposes in several circumstances: early viremia, maternal antibodies.
Guiot
A L
AL
Rhône Mérieux, Laboratoire de Lyon, France.
Rigal
D
D
Pialot
D
D
Chappuis
G
G
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001800:Blood Specimen Collection / Q000662:veterinary; D002415:Cats; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D016181:Feline Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis*; D016089:Immunodeficiency Virus, Feline / Q000302:isolation & purification*; D008214:Lymphocytes / Q000821:virology*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013997:Time Factors; D014766:Viremia / Q000097:blood / Q000175:diagnosis*
D000956:Antigens, Viral