Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline-rich proteins (PRPs).
Beeley
J A
JA
Department of Oral Sciences, University of Glasgow Dental School, UK.
Newman
F
F
Wilson
P H
PH
Shimmin
I C
IC
D003619:Dansyl Compounds; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D010306:Parotid Gland / Q000378:metabolism*; D010455:Peptides / Q000032:analysis; D055232:Proline-Rich Protein Domains; D012394:Rosaniline Dyes; D012463:Saliva / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity; D016622:Silver Staining; D000516:alpha-Amylases / Q000032:analysis
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010455:Peptides; D012394:Rosaniline Dyes; D012471:Salivary Proteins and Peptides; C024757:Coomassie brilliant blue R; D000516:alpha-Amylases; C029038:dansyl chloride