Relationship of early follicular phase sera follicle stimulating hormone and luteinizing hormone levels as measured by a radioimmunoassay and an enzyme-linked immunosorbent assay to number of oocytes retrieved.
A study was performed to see if the level of serum follicle stimulating hormone (FSH) or luteinizing hormone (LH) obtained in the early follicular phase could predict the number of oocytes retrieved following in vitro fertilization (IVF). For each patient the sera FSH and LH were measured by both an enzyme-linked immunosorbent assay (ELISA) and a radioimmunoassay (RIA) method. With the ELISA method when early follicular phase serum FSH was < or = to the group median (9.0 mIU/mL) 16.5 oocytes were retrieved vs 6.7 when FSH was greater than the median. Comparable values using the median of the RIA assay were 17.5 vs 8.1 oocytes. Similar analysis for serum LH failed to show any relationship between baseline LH and the number of oocytes retrieved. This study thus demonstrates that at least one non-isotopic method is equal to a specific RIA method in distinguishing good from average or poor responders.
Check
J H
JH
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, USA.
Nazari
A
A
Kuhn
R
R
Lauer
C
C
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000097:blood*; D005498:Follicular Phase / Q000097:blood*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology*; D010053:Ovary / Q000502:physiology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011863:Radioimmunoassay / Q000379:methods; D013480:Superovulation / Q000097:blood / Q000502:physiology
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone