title: Preprovasopressin mRNA is not present in dorsal root ganglia of the rat.
abstract: Immunohistochemical studies on colchicine-treated rats have suggested that more than half of the neurons in dorsal root ganglia (DRG) contain vasopressin. Thus, vasopressin would be the most commonly found peptide in DRG neurons. In the present study we have reexamined the presence of vasopressin in DRG neurons, using a sensitive in situ hybridization method employing long riboprobes that will detect very small amounts of mRNA. The C3, C6, T2, T12, L2 and L5 DRG were studied. None of these ganglia contained any preprovasopressin mRNA. Yet, dense labeling for preprovasopressin mRNA was seen on simultaneously processed hypothalamic sections and a heavy preprotachykinin mRNA expression was seen in adjacent DRG sections. These findings demonstrate that vasopressin is not produced in DRG in normal rats.
authors: Department of Cell Biology, Faculty of Health Sciences, University of Linköping, Sweden. martin.hallbeck@mcb.liu.se
authors: M
authors: M
authors: Hallbeck
authors: O
authors: O
authors: Hermanson
authors: A
authors: A
authors: Blomqvist
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; C091487:preprovasopressin; D014667:Vasopressins