Prostaglandin H synthase-2 in human gestational tissues: regulation in amnion.
Using reverse transcriptase-polymerase chain reaction we have established that mRNAs for prostaglandin H synthases 1 and 2 (PGHS-1 and PGHS-2) are present in amnion, chorion and decidua from women both at term before and after the onset of labour and from women at 28-35 weeks of gestation before the onset of labour. By Western blot analyses we have demonstrated that epidermal growth factor, interleukin 1 beta and phorbol 12-myristate 13-acetate all increase PGHS-2 amounts in amnion cells. The degree of stimulation caused by these substances (218-311 per cent) is less than the increase in prostaglandin production usually generated (five- to 10-fold). Hence we believe that these substances may have multiple sites of action in the pathways of arachidonic acid metabolism.
Trautman
M S
MS
Department of Pediatrics, University of Utah, USA.
Edwin
S S
SS
Collmer
D
D
Dudley
D J
DJ
Simmons
D
D
Mitchell
M D
MD
D000650:Amnion / Q000201:enzymology*; D002823:Chorion / Q000201:enzymology*; D003656:Decidua / Q000201:enzymology*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases