Immunohistochemical evaluation of nm23-H1 gene product in transitional cell carcinoma of the bladder.
The expression of the nm23-H1 gene has been suggested to have an inverse association with metastases in certain tumours. The aim of this study was to investigate the relationship of nm23-H1 immunohistochemical expression with pathological tumour variables and survival in a series of transitional cell carcinomas (TCCs) of the bladder. Formalin-fixed, paraffin-embedded archival tissue from 87 carcinomas (Ta-T1 45 cases) and T2-T4 (42 cases) was immunostained (Strept ABC/HRP) with the NDPK-A monoclonal antibody (NDPK-A) against nm23-H1 protein. The tumours had already been evaluated for immuno-expression of p53 protein. In addition, DNA analysis was performed by flow cytometry. Results were analysed using the linear trend in proportions test, the Fisher's exact test and multivariate analysis. Paradoxically, advanced tumour stage showed significant correlation with nm23-H1 immunopositivity in muscle invasive TCCs (P(t) = 0.01). Patients with nm23-H1 positive, muscle invasive TCCs had a worse prognosis at a level of suggestive statistical significance (PF = 0.08). In multivariate analysis, using a Cox's proportional hazards survival model with six variables, tumour grade, disease stage and synchronous p53 and nm23-H1 detection showed significant correlation with poor patient survival (P = 0.014, P = 0.049 and P = 0.05, respectively).
Nakopoulou
L L
LL
Department of Pathology, Medical School of Athens University, Greece.
Constandinides
C A
CA
Tzonou
A
A
Lazaris
A C
AC
Zervas
A
A
Dimopoulos
C A
CA
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002295:Carcinoma, Transitional Cell / Q000276:immunology / Q000378:metabolism* / Q000401:mortality; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase; D015995:Prevalence; D011379:Prognosis; D014157:Transcription Factors; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000401:mortality
D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins