title: Recruitment of damaged DNA to the nuclear matrix in hamster cells following ultraviolet irradiation.
abstract: We examined the relationship between the nuclear matrix and DNA in the dihydrofolate reductase domain following irradiation of Chinese hamster cells with UV light. The fraction of matrix-bound DNA increased in transcribed and non-transcribed regions during a 3 h period after irradiation. However, no increase was observed with excision repair-deficient cells mutant for the ERCC1 gene. The major UV-induced lesion, the cyclobutane pyrimidine dimer, increased in frequency in the matrix-bound DNA 1 h after irradiation, in both transcribed and non-transcribed regions, but decreased subsequently. This phenomenon was also lacking in excision repair-deficient cells. These data demonstrate that recruitment of lesion-containing DNA to the nuclear matrix occurs following UV irradiation and suggest that this recruitment is dependent upon nucleotide excision repair. This is consistent with the concept of a 'repair factory' residing on the nuclear matrix at which excision repair occurs.
authors: Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
authors: D R
authors: DR
authors: Koehler
authors: P C
authors: PC
authors: Hanawalt
mesh_terms: D000818:Animals; D016466:CHO Cells; D002451:Cell Compartmentation; D006224:Cricetinae; D004249:DNA Damage; D004260:DNA Repair; D015530:Nuclear Matrix / Q000378:metabolism*; D013347:Subcellular Fractions; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D013762:Tetrahydrofolate Dehydrogenase