Toward fully automated high-resolution electron tomography.
Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT system, which automates both tomographic data collection and reconstruction.
Fung
J C
JC
Graduate Group in Biophysics, University of California at San Francisco 94143-0448, USA.
Liu
W
W
de Ruijter
W J
WJ
Chen
H
H
Abbey
C K
CK
Sedat
J W
JW
Agard
D A
DA
D000818:Animals; D001331:Automation; D018385:Centrosome / Q000648:ultrastructure; D003196:Computer Graphics; D003198:Computer Simulation; D008854:Microscopy, Electron; D008961:Models, Structural; D015388:Organelles / Q000648:ultrastructure*; D012984:Software