Alteration of collagen biosynthesis and analysis of type I and type III collagens of prostate in young rats following sex hormone treatments.
Two series of experiments were conducted to clarify the collagen biosynthesis and isolation of type I and type III collagens of young rats that received various sex hormone treatments. Evidence has been presented that (1) estradiol-17 beta treatment induces prostatic atrophy and suppresses the incorporation of [3H]proline into collagen in prostate; (2) administration of estradiol-17 beta increases the collagen content in the prostate; (3) localization of type I and III collagens in the interstitium of the prostate is detected by indirect immunofluorescence staining; (4) the ratio of type III collagen to type I collagen in the gland decreases following estradiol-17 beta treatment; and (5) neither treatment with testosterone nor administration of testosterone plus estradiol-17 beta in precastrated rats shows discernible effects on these valuables. These findings suggest that estradiol-17 beta increases the accumulation of collagen into the prostate with different extents of influence on the synthesis of type I and III collagens.
Suzuki
H
H
Department of Urology, Yamagata University School of Medicine, Japan.
Nakada
T
T
D000818:Animals; D003094:Collagen / Q000032:analysis* / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D011467:Prostate / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology*; D014018:Tissue Distribution
D013739:Testosterone; D004958:Estradiol; D003094:Collagen