Cloning and expression of canine interleukin-10.
We describe here the cloning of canine IL-10 cDNA (GenBank accession No. U33843) and the expression of recombinant IL-10 in a dog kidney cell line (DK6247) and Chinese hamster ovary cells (CHO). Canine IL-10 exhibits strong sequence homology to the known sequences of human, mouse, rat, and bovine genes at nucleotide and amino acid levels. The IL-10 gene, when introduced into DK and CHO cell lines, produces recombinant IL-10 that causes an inhibitory effect on allogeneic MHC-driven lymphoproliferative responses.
Lu
P
P
Department of Surgery, University of Miami School of Medicine, FL 33101, USA.
Zucker
K
K
Fuller
L
L
Tzakis
A
A
Esquenazi
V
V
Miller
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification; D004285:Dogs; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics*; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D011994:Recombinant Proteins; D016753:Interleukin-10