title: The impact of woman's age and sperm parameters on fertilization rates in IVF cycles.
abstract: OBJECTIVE
  To examine the predictive value of different sperm parameters and the age of a couple on fertilization rate.
  
  
  METHODS
  One-hundred six consecutive sperm samples were analysed before insemination of oocytes in IVF cycles. Sperm count and motility were examined with the Makler Counter Chamber. Morphology was evaluated by the eosin nigrosin and giemsa stains. Membrane integrity was evaluated by the hypoosmotic swelling test (HOST).
  
  
  RESULTS
  Using a step-wise logistic regression test the following parameters were all significantly correlated with the fertilization rates: sperm concentration after a 3-h layering (chi 2 = 15.85, P < 0.001), sperm motility index (chi 2 = 6.12, P < 0.0134), woman's age (chi 2 = 6.25, P < 0.012), and the hypoosmotic test (chi 2 = 6.63, P < 0.01). A fertilization capacity formula was established by using these four parameters to predict the fertilization capacity of a couple. Significantly lower fertilization rates were detected in elderly women with the partner sperm concentration of 7 x 10(6)/ml after 3-h layering, < 30% swollen spermatozoa in the HOST and motility index score of < 50.
  
  
  CONCLUSIONS
  By the aforementioned formula, sperm fertilization capacity can be predicted by using simple sperm parameters and women's age. Elderly women with partners who have suboptimal sperm parameters reflected by low fertilization capacity scores may benefit from micro-manipulation procedures.
authors: Department of Obstetrics and Gynecology, Hasharon Hospital, Golda Medical Center, Petah Tikva, Israel.
authors: J
authors: J
authors: Ashkenazi
authors: R
authors: R
authors: Orvieto
authors: R
authors: R
authors: Gold-Deutch
authors: D
authors: D
authors: Feldberg
authors: D
authors: D
authors: Dicker
authors: I
authors: I
authors: Voliovitch
authors: Z
authors: Z
authors: Ben-Rafael
mesh_terms: D000328:Adult; D000367:Age Factors; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013075:Sperm Capacitation; D013076:Sperm Count; D013081:Sperm Motility