Serum samples from pancreatectomized patients contain trypsinogen immunoreactivity.
The concentrations of trypsinogen-1 and -2 in serum samples from patients who have undergone pancreatectomy were measured by highly sensitive and specific time-resolved immunofluorometric assays. The isoenzyme pattern was determined by ion-exchange chromatography and determination of immunoreactivity in the fractions. All samples contained trypsinogen-2, the mean level being one fifth of that in healthy controls. Trypsinogen-1 was detected in one of nine samples. In addition to the main trypsinogen isoenzymes, we observed in normal serum two trypsinogen isoenzymes previously found in mucinous ovarian cyst fluid. Our results suggest that trypsinogen is not exclusively expressed by the pancreas and certain tumors but that it also may be produced by normal extrapancreatic tissues. This should be considered when an assay of trypsinogen in serum is used for clinical purposes.
Itkonen
O
O
Department of Clinical Chemistry, Helsinki University Central Hospital, Finland.
Stenman
U H
UH
Osman
S
S
Koivunen
E
E
Halila
H
H
Schröder
T
T
D000230:Adenocarcinoma / Q000378:metabolism; D000328:Adult; D000368:Aged; D002852:Chromatography, Ion Exchange; D003110:Colonic Neoplasms / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D010048:Ovarian Cysts / Q000378:metabolism; D010180:Pancreatectomy; D010195:Pancreatitis / Q000097:blood; D011184:Postoperative Period; D012016:Reference Values; D014362:Trypsinogen / Q000097:blood*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D007527:Isoenzymes; D014362:Trypsinogen