Evidence for phospholipase B activity in Fusobacterium necrophorum cultures and its association with hemolysin/leucocidin activities.
Phospholipase B (PLB) activity was present in Fusobacterium necrophorum cultures and it correlated closely with virulence and co-purified with the hemolysin/leucocidin activities. All three activities were associated with a large molecule or molecular complex (6 x 10(2)-2 x 10(3) kDa) exhibiting varying degrees of aggregation. These were present mainly in the culture medium and to a lesser extent in cell sonicates. The PLB and toxin activities were sensitive to heat, dissociating agents, proteolytic enzymes, prolonged purification regimes, freeze-drying and repeated freeze-thawing. The toxin(s) were stable over a broad range of pH, did not require divalent ions or reducing agents and could be kept for several months as an ammonium sulfate precipitate at 4 degrees C, or stored as a concentrated liquid in the presence of proteolytic inhibitors at - 20 degrees C.
Fifis
T
T
Commonwealth Scientific and Industrial Research Organization, Division of Animal Health, Parkville, Vic, Australia.
Costopoulos
C
C
Vaughan
J A
JA
D000818:Animals; D002417:Cattle; D005675:Fusobacterium necrophorum / Q000201:enzymology / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007956:Leukocidins / Q000302:isolation & purification / Q000378:metabolism*; D008245:Lysophospholipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
D006460:Hemolysin Proteins; D007956:Leukocidins; D008245:Lysophospholipase