Analysis of human interferon-alpha gene promoters by multiple sequence alignment.
A map of conserved motifs within 11 human interferon-alpha gene promoters was developed using multiple sequence alignment. Multiple sequence alignment revealed the highly conserved structure of the virus-response element and a second region immediately upstream of the tata box. Homolog analysis indicated six families of potential promoter elements: (1) tata boxes, (2) GTATGT motifs, (3) virus-response elements, (4) distal putative interferon regulatory factor binding sites, (5) proximal putative interferon regulatory factor binding sites, and (6) TG sequences. On the basis of conserved promoter elements, a theoretical model of human interferon-alpha gene promoter organization was advanced. Transcriptional regulation of human interferon-alpha gene expression is controlled by a tata box and four regulatory domains. Experimental verification is necessary to confirm the predicted transcriptional control sequences. Proposed experimentation will generate insight into the differential transcriptional regulation of human interferon-alpha gene expression.
Houle
J L
JL
School of Computer Science, Carleton University, Ottawa, Ontario, Canada.
Santoro
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D005809:Genes, Regulator; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D016898:Interferon-alpha