Molecular diversity of GABA-gated chloride channels in the rat anterior pituitary.
mRNA expression of GABA-gated Cl(-)-channels in rat antepituitary was evaluated by using an reverse-transcribed (RT)-polymerase chain reaction (RT-PCR) method with degenerate and specific oligonucleotides. The main result of our findings is that the antepituitary expresses mRNAs encoding alpha 4 and rho 1 GABA receptor subunits. These two subunits are believed to be, respectively, constituents of benzodiazepine-insensitive GABAA and GABAC receptors in the CNS. This molecular analysis is consistent with the pharmacological diversity of GABA receptors in pituitary cells.
Boué-Grabot
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E
Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II, France.
Dufy
B
B
Garret
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M
D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000502:physiology*; D005260:Female; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000235:genetics*; D014158:Transcription, Genetic; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D018118:Chloride Channels; D010446:Peptide Fragments; D012333:RNA, Messenger; D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid