Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency.
A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standard were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.
Grubb
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NG
Department of Medicine, Indiana University School of Medicine, Roudebush Veterans Affairs Medical Center, Indianapolis 46202, USA.
Rudy
D W
DW
Hall
S D
SD
D000215:Acylation; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D015314:Dialysis Solutions / Q000737:chemistry; D005965:Glucuronates / Q000032:analysis / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007660:Ketoprofen / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood*; D007700:Kinetics; D051437:Renal Insufficiency / Q000097:blood*; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013237:Stereoisomerism
D015314:Dialysis Solutions; D005965:Glucuronates; C044223:ketoprofen glucuronide; D007660:Ketoprofen