Transfer of bovine microsatellites to the cervine (Cervus elaphus).
Bovine microsatellites were used to amplify DNA of red deer (Cervus elaphus). Fourteen of 27 bovine systems (52%) displayed polymorphism, while no (CA)n-repeat was detected in seven systems and six systems gave no amplificates in red deer. The allele number ranged from 2 to 7, the polymorphism information content between 0.24 and 0.76. The results demonstrate that transfer of microsatellite systems between families of the same order (artiodactyla) is possible. Molecular genetic research will help to clarify the differentiation and ecology of wild animals and will contribute to define criteria needed for the preservation of endangered species.
Kühn
R
R
Institut für Tierwissenschaften der TU München Freising-Weihenstephan, Germany.
Anastassiadis
C
C
Pirchner
F
F
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D003670:Deer / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D005828:Genetics, Population; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
D005819:Genetic Markers; D004247:DNA