Effects of dipfluzine on cortical somatosensory evoked potentials and amino acid contents in ischemic rat brain.
OBJECTIVE
To evaluate the effects of dipfluzine ¿1-diphenyl-methyl-4-[3-(4-fluorobenzoyl)]-piperazine, Dip¿ on the intra- and extra-cellular contents of the amino acids in brain and the cortical somatosensory evoked potentials (SEP) in rats with cerebral ischemia.


METHODS
Amino acids in micro-dialysates and brain tissue homogenates in female Wistar rats with bilateral carotid artery ligation (BCAL) were measured by HPLC and SEP was measured by the electrophysiological technique.


RESULTS
Dip 50 mg.kg-1 i.p. prevented SEP from prolonging of the latency and overactivity of lowered amplitude, markedly lowered the elevation in extracellular level of glutamate (Glu), aspartate (Asp), and glycine (Gly) in intracerebral microdialysates, and alleviated the decrement of intracellular contents of Glu, Asp, Gly, taurine (Tau), and GABA in brain tissue.


CONCLUSIONS
Dip reduced the disturbance of cortical function and the imbalance between excitatory and inhibitory amino acids in ischemic brain, therefore provided a further evidence for its protective effect on ischemic cerebral damage.
Wang
Y L
YL
Department of Pharmacology, Hebei Medical College, Shijiazhuang, China.
He
R R
RR
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000503:physiopathology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002936:Cinnarizine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D005260:Female; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D018846:Excitatory Amino Acids; D002936:Cinnarizine; C065758:dipfluzine