High-performance liquid chromatographic determination of phenylephrine in human serum with coulometric detection.
A high-performance liquid chromatographic method for the determination of phenylephrine (PE) in human serum using coulometric detection is described. PE and internal standard, orciprenaline, were extracted from serum by solid-phase extraction and separation achieved on a coupled column system consisting of two C18 cartridge columns (250 x 4.6 mm I.D. coupled to a shorter 50 x 4.6 mm I.D. column) using a mobile phase of methanol-50 mM phosphate buffer (pH 3.2; 10:90) at 36 degrees C. Dual electrode coulometric detection was used in the "oxidative screen" mode. Calibration curves were linear over the range 0.3-4 ng/ml with a limit of quantification (LOQ) of 0.35 ng/ml. The method has a greater degree of sensitivity, precision and accuracy compared to previously published methods for PE and is suitable for use in pharmacokinetic and bioequivalence studies in humans.
Vuma
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Biopharmaceutics Research Institute, School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Kanfer
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D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D004563:Electrochemistry; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D010656:Phenylephrine / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013810:Therapeutic Equivalency
D010656:Phenylephrine