The mus-8 gene of Neurospora crassa encodes a structural and functional homolog of the Rad6 protein of Saccharomyces cerevisiae.
We cloned a DNA repair gene, mus-8, of Neurospora crassa and sequenced the genomic DNA and cDNA. Nucleotide-sequence analysis indicated that the mus-8 gene contains an open reading frame (ORF) of 456 bp, interrupted by three small introns. The deduced amino-acid sequence showed that the mus-8 gene encodes a 17 kDa protein which has 77.5% and 83.3% identity to the Rad6 protein of Saccharomyces cerevisiae and the rhp6(+) protein of Schizosaccharomyces pombe, respectively. The Rad6 protein is a ubiquitin-conjugating enzyme (E2) and is required for DNA repair, mutagenesis, and sporulation in yeast. Introduction of the mus-8 gene into a S. cerevisiae rad6 mutant resulted in significant recovery of DNA repair functions, especially UV-mutagenesis, and also sporulation, both of which are defective in the rad6 mutant. It is therefore postulated that mus-8 of Neurospora has a function very similar to that demonstrated for RAD6 of S. cerevisiae.
Soshi
T
T
Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University, Urawa 338 Japan.
Sakuraba
Y
Y
Käfer
E
E
Inoue
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005656:Fungal Proteins; D005800:Genes, Fungal; D008025:Ligases / Q000235:genetics*; D008741:Methyl Methanesulfonate; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009492:Neurospora crassa / Q000235:genetics* / Q000528:radiation effects; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D044763:Ubiquitin-Conjugating Enzymes; D014466:Ultraviolet Rays
D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D008741:Methyl Methanesulfonate; C101599:Mus-8 protein, Neurospora crassa; C471606:RAD6 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases; D008025:Ligases