[Immunosuppressive effect of intravenously injected docosahexaenoic acid on single lung allotransplantation in the rat].
Docosahexaenoic acid (DHA) is an omega 3 polyunsaturated fatty acid. The purpose of this study was to evaluate the immunosuppressive effect of intravenously injected DHA in rat orthotopically transplanted allograft lung. Based on the results of the post-operative treatment, the recipients were divided into four groups; Group A (n = 3): normal. Group B (n = 3): isotransplantation with no immunosuppressive drugs. Group C (n = 6): allotransplantation with no immunosuppressive drugs. Group D-1 (n = 3): allotransplantation with DHA at a dose of 0.5 g/kg/day x 2 at 0 and 3 post-operative days (POD). Group D-2 (n = 3): allotransplantation with DHA at a dose of 0.5 g/kg/day x 4 at 0, 1, 3 and 5 POD. Group D-3 (n = 6): allotransplantation with DHA at a dose of 0.5 g/kg/day x 4 at 0, 1, 2 and 3 POD. In Groups A and B, F344 rats were used as donor and recipient. In Groups C and D, F344 rats were used as recipient and WKAH rats as donor. Studies on morphology, bronchoalveolar lavage (BAL) and peripheral blood cells were performed at 7 POD after sacrifice. Histological changes in Group D-3 were milder than those in Grop C and D-1, but were more progressive than those in Groups A and B which showed no rejection. Peripheral leukocytes and polymorphonuclear leukocytes counts in Groups D-1, D-2, and D-3 were higher than those in Groups, A, B and C. Total cells and lymphocytes of BAL in Groups D-2 and D-3 were less than those in Group C, but total cells and polymorphonuclear leukocytes of BAL in Groups D-2 and D-3 were more than those in Groups A and B. This study suggested that intravenously injected DHA had an immunosuppressive effect on the allotransplanted lung in the rats.
Sasaki
H
H
First Department of Surgery, Gifu University School of Medicine, Japan.
Hirose
H
H
Sakai
S
S
Zhang
Y Q
YQ
Hamazaki
T
T
D000818:Animals; D018893:Bronchoalveolar Lavage; D016516:CD4-CD8 Ratio; D004281:Docosahexaenoic Acids / Q000008:administration & dosage*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D007275:Injections, Intravenous; D008168:Lung / Q000276:immunology / Q000473:pathology; D016040:Lung Transplantation / Q000379:methods; D018655:Lymphocyte Count; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D007166:Immunosuppressive Agents; D004281:Docosahexaenoic Acids