title: Determination of alosetron in human plasma or serum by high-performance liquid chromatography with robotic sample preparation.
abstract: A method of analysis for the determination of alosetron in human plasma or serum has been developed. The method was fully automated using a laboratory robot in order to improve analytical precision, efficiency and safety. The assay involved solid-phase extraction with reversed-phase HPLC separation and fluorescence detection. A validation exercise over the concentration range of 0.1 to 20 ng/ml demonstrated the selectivity, linearity, sensitivity, accuracy, precision, extraction efficiency, ruggedness and stability of the method. The method has been applied in support of numerous human pharmacokinetic/biopharmaceutic studies over the last five years.
authors: Glaxo Research Institute, Research Triangle Park, NC 27709, USA.
authors: T L
authors: TL
authors: Lloyd
authors: S K
authors: SK
authors: Gupta
authors: A E
authors: AE
authors: Gooding
authors: J R
authors: JR
authors: Alianti
mesh_terms: D002243:Carbolines / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry; D012371:Robotics; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D002243:Carbolines; D012702:Serotonin Antagonists; C090840:alosetron