title: D-glucose metabolism in BRIN-BD11 islet cells.
abstract: A novel insulin-secreting cell line, BRIN-BD11, was recently established following electrofusion of RINm5F cells with NEDH rat pancreatic islet cells. In the present study, D-glucose metabolism was compared in BRIN-BD11 and RINm5F cells. The concentration dependency of D[5-3H]glucose utilization displayed a comparable pattern in the two cell lines, but the absolute values were lower in BRIN-BD11 than RINm5F cells. Except in the case of D-[1-14C]glucose, the ratio between 14C labeled D-glucose oxidation and D-[5-3H]glucose utilization was higher, however, in BRIN-BD11 than RINm5F cells. Moreover, BRIN-BD11 cells were less affected than RINm5F cells by a rise in D-glucose concentration, in terms of the inhibitory action of the hexose upon oxidative variables, such as oxidative glycolysis, pyruvate decarboxylation, and oxidation of glucose-derived acetyl residues in the Krebs cycle. The total energy yield from D-glucose catabolism appeared similar, however, in BRIN-BD11 and RINm5F cells. These findings extend the knowledge that BRIN-BD11 cells display an improved metabolic and secretory behavior, when considering the difference otherwise found between normal and tumoral islet cells.
authors: Laboratory of Experimental Medicine, Brussels Free University, Belgium.
authors: J
authors: J
authors: Rasschaert
authors: P R
authors: PR
authors: Flatt
authors: C R
authors: CR
authors: Barnett
authors: N H
authors: NH
authors: McClenaghan
authors: W J
authors: WJ
authors: Malaisse
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002952:Citric Acid Cycle; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006019:Glycolysis; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D051381:Rats; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D014316:Tritium; D005993:Glycerolphosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005947:Glucose