Cardiotrophin-1 is not the sweat gland-derived differentiation factor.
Sympathetic neurons innervating sweat glands undergo a target-directed switch in neurotransmitter properties. Although the factor responsible for inducing this switch has not been identified, it appears to be a member of the neuropoietic cytokine family. Cardiotrophin-1 (CT-1), a new family member, was analyzed to determine whether it was a relevant factor. CT-1 induced choline acetyl-transferase and vasoactive intestinal peptide in cultured sympathetic neurons, and RT/PCR amplified CT-1 mRNA from footpad total RNA. The differentiation activity of CT-1 was blocked by CT-1 antiserum. The activity in sweat gland extracts and cultures was not, however, suggesting that CT-1 is not the sweat gland-derived factor.
Habecker
B A
BA
Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Pennica
D
D
Landis
S C
SC
D000818:Animals; D001483:Base Sequence; D001685:Biological Factors / Q000032:analysis* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D016207:Cytokines / Q000032:analysis* / Q000502:physiology; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D051381:Rats; D013545:Sweat Glands / Q000294:innervation*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D001685:Biological Factors; D016207:Cytokines; D014660:Vasoactive Intestinal Peptide; C092073:cardiotrophin 1; D002795:Choline O-Acetyltransferase