title: Corticotropin-releasing factor up-regulates its own receptor mRNA in the paraventricular nucleus of the hypothalamus.
abstract: We investigated the role of CRF in regulating receptor expression in the paraventricular nucleus (PVN). First, to clarify the effect of exogenously administered CRF, 1 microgram of ovine CRF was injected into rat lateral ventricle and changes in concentration of the CRF type 1 receptor (CRF1-R) and CRF mRNA in the PVN were semiquantified after in situ hybridization. Second, we determined the effect of stress, as a stimulant of endogenous CRF secretion, on mRNA accumulation. While CRF1-R mRNA expression was low to be undetectable in the PVN of controls, both intracerebroventricular administration of CRF and restraint significantly increased CRF1-R and CRF signals in the parvocellular PVN. Thus CRF may modulate CRF production and release from the PVN, by regulating CRF1-R expression.
authors: Department of Medicine, Tokyo Women's Medical College, Japan.
authors: T
authors: T
authors: Imaki
authors: M
authors: M
authors: Naruse
authors: S
authors: S
authors: Harada
authors: N
authors: N
authors: Chikada
authors: J
authors: J
authors: Imaki
authors: H
authors: H
authors: Onodera
authors: H
authors: H
authors: Demura
authors: W
authors: W
authors: Vale
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D015870:Gene Expression; D007276:Injections, Intraventricular; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics*; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone