title: Long-term low ascorbic acid intake reduces bone mass in guinea pigs.
abstract: The effect of long-term (1 y) low to excess ascorbic acid (AA) intake on bone mass was evaluated using guinea pigs that were 12-14 d old at the start of the experiment. Dietary AA was low (0.15 g/ kg diet) (n = 7), normal (0.50 g/kg) (n = 8) or excess (10 g/kg) (n = 8). After 12 mo, total body bone mineral density (BMD, mg/cm2) and bone mineral content (BMC, g) were determined by dual energy X-ray absorptiometry. Histomorphometric analysis of the cancellous bone of the proximal tibial metaphysis was completed after in vivo dual fluorochrome labeling. Total body BMD of the low AA group was 4.9% lower (P < 0.05), and total body BMC was 12.4% lower (P < 0.05) than in the normal AA group. Total body BMD and BMC were similar in normal and excess AA groups and in the low and excess AA groups. Histomorphometric analysis indicated significantly greater (P < 0.05) double-labeled bone surface, mineralizing surface, and bone formation rate in the low AA guinea pigs compared with the normal AA animals. Thus, there was greater bone turnover in the low AA group than in the normal AA guinea pigs. No differences in histomorphometric endpoints existed between the normal AA and excess AA groups. Long-term AA deficiency, during the period of rapid growth and slower phases of skeletal maturation, resulted in bone abnormalities in adult guinea pig skeletons. Long-term dietary AA excess caused no such abnormalities.
authors: Departments of Dietetics and Nutrition, University of Kansas Medical Center, Kansas City 66160, USA.
authors: D E
authors: DE
authors: Kipp
authors: C E
authors: CE
authors: Grey
authors: M E
authors: ME
authors: McElvain
authors: D B
authors: DB
authors: Kimmel
authors: R G
authors: RG
authors: Robinson
authors: B P
authors: BP
authors: Lukert
mesh_terms: D015502:Absorptiometry, Photon; D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology; D001835:Body Weight / Q000502:physiology; D015519:Bone Density / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D008099:Liver / Q000737:chemistry; D013977:Tibia / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
chemical_list: D001205:Ascorbic Acid; D002118:Calcium