Construction of eukaryotic expression vector pBlacz and its expression both in vitro and in vivo.
A novel eukaryotic expression vector pBlacZ was constructed, which was transfected into the cell lines of NIH/3T3, COS-1, CHO and the primary culture of murine dermatic fibroblasts in vitro, and also into the murine subcutaneous layer and skeletal muscles of rats in vivo. It was detected that the gene expression vector could encode the E. Coli beta-galactosidase effectively in all these histocytes. The results suggested that pBlacZ, as a novel expression vector, might have certain value of application.
Qu
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Department of Biochemistry, Tongji Medical University, Wuhan.
Yang
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S
You
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Y
Liu
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He
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D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology; D005057:Eukaryotic Cells; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D007763:Lac Operon; D051379:Mice; D051381:Rats; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis*
D004274:DNA, Recombinant; D001616:beta-Galactosidase