title: The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program.
abstract: OBJECTIVE
  To study the possible influence of antioxidant treatment on human spermatozoa and the fertilization rate in an IVF program.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  In Vitro Fertilization Unit, Serlin Maternity Hospital, and the Laboratory of Male Fertility, Bar-Ilan University, Ramat-Gan, Israel.
  
  
  METHODS
  Fifteen fertile normospermic male volunteers who had low fertilization rates in their previous IVF cycles.
  
  
  METHODS
  Vitamin E (alpha-tocopherol) 200 mg daily by mouth for 3 months.
  
  
  METHODS
  Lipid peroxidation potential (amount of malondialdehyde [MDA]), quantitative ultramorphologic analysis of spermatozoa, and fertilization rate per cycle.
  
  
  RESULTS
  The high MDA levels significantly decreased from 12.6 +/- 9.4 nmol/10(8) spermatozoa to normal levels of 7.8 +/- 4.2 nmol/10(8) spermatozoa after 1 month of treatment. The fertilization rate per cycle increased significantly from 19.3 +/- 23.3 to 29.1 +/- 22.2 after 1 month of treatment. No additional effects on MDA levels and fertilization rate were observed after completion of treatment. With regard to the quantitative ultramorphologic analysis, none of the sperm cell subcellular organelles were affected significantly by vitamin E treatment.
  
  
  CONCLUSIONS
  Vitamin E may improve the fertilization rate of fertile normospermic males with low fertilization rates after 1 month of treatment, possibly by reducing the lipid peroxidation potential, and with no change of the quantitative ultramorphologic analysis of subcellular organelles.
authors: Serlin Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Israel.
authors: E
authors: E
authors: Geva
authors: B
authors: B
authors: Bartoov
authors: N
authors: N
authors: Zabludovsky
authors: J B
authors: JB
authors: Lessing
authors: L
authors: L
authors: Lerner-Geva
authors: A
authors: A
authors: Amit
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects* / Q000502:physiology; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D015388:Organelles / Q000648:ultrastructure; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde