Desferrioxamine-dependent iron transport in Erwinia amylovora CFBP1430: cloning of the gene encoding the ferrioxamine receptor FoxR.
Iron deprivation of Erwinia amylovora CFBP1430, a species causing fire blight on Pomoïdeae, was shown to induce the production of siderophores of the desferrioxamine (dfo) family and two outer membrane polypeptides with apparent molecular weight of about 70 and 80 kDa, respectively. Cyclic dfo E was characterized as the major metabolite. Phage MudIIpR13 insertional mutagenesis and screening on CAS-agar medium yielded three dfo non-producing and one overproducing clones. These clones failed to grow in the presence of the Fe(III) chelator EDDHA and were determined further as dfo and ferrioxamine transport negative mutants, respectively. The transport mutant which appeared to lack the 70 kDa polypeptide in the outer membrane allowed the purification of dfo E. Growth under iron limitation of dfo negative mutants was stimulated with ferrioxamine E and B but not with other ferrisiderophores tested. The host DNA sequence flanking the left terminal part of the MudIIpR13 prophage responsible for the transport mutation was cloned and used to probe a parental gene library by DNA-DNA hybridization. Two recombinant cosmids restoring the transport mutation to normal were identified. Both cosmids also conferred the ability to utilize ferrioxamine B and E as iron sources on a FhuE- mutant of Escherichia coli. This correlated with the production of an additional polypeptide of 70 kDa in the outer membrane of E. coli transconjugants, thus confirming that this protein serves the ferrioxamine receptor function (FoxR) in E. amylovora.
Kachadourian
R
R
Laboratoire de Chimie Bioorganique et Bioinorganique, Université Paris-Sud, Orsay, France.
Dellagi
A
A
Laurent
J
J
Bricard
L
L
Kunesch
G
G
Expert
D
D
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D003676:Deferoxamine / Q000494:pharmacology*; D004885:Erwinia / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D008970:Molecular Weight; D016254:Mutagenesis, Insertional; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C489523:FoxB protein, E coli; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C072460:foxR protein, bacteria; D007501:Iron; D003676:Deferoxamine