Proteins that smell: pheromone recognition and signal transduction.
Pheromone perception in Lepidoptera requires initial recognition and transport of the pheromone molecule by ligand-specific pheromone binding proteins (PBPs) in the moth antennae, followed by recognition of the ligand or PBP-ligand complex by a transmembrane G-protein-coupled odorant receptor protein. This signal is transduced by activation of a specific phospholipase C, intracellular release of inositol 1,4,5-trisphosphate (IP3) and IP3-gated opening of an ion channel. Individual pheromone-specific PBPs provide the initial ligand recognition event and encode ligand specificity. We have used photoaffinity labeling, cDNA library screening and cloning, protein expression, a novel binding assay and site-directed mutagenesis to define the ligand specificity of PBPs.
Prestwich
G D
GD
Department of Chemistry, University at Stony Brook, NY 11794-3400, USA.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000502:physiology*; D010675:Pheromones / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D012903:Smell / Q000502:physiology*; D013329:Structure-Activity Relationship
D010675:Pheromones