Candida albicans stress mannoproteins expression in superficial and systemic candidiasis. Stress mannoproteins in Candida albicans.
The presence of heat shock mannoproteins (HSMPs) reactive with sIgA was demonstrated in several C. albicans strains. The subculture of the C. albicans isolated from mucosal surfaces on Sabouraud's dextrose agar at 25 degrees C switched off the HSMP expression. A re-expression of the HSMPs was obtained in the same medium by shifting the temperature of incubation to 37 degrees C. However, expression of HSMPs in two strains isolated from deep infections was maintained during several subcultures on Sabouraud's dextrose agar at 25 degrees C. A glycoprotein of 200 kDa seemed to be the main HSMP reacting with vaginal sIgA. The data presented in this study suggest that factors other than temperature can influence the expression of C. albicans HSMPs and therefore these antigens should be referred as stress mannoproteins.
Pontón
J
J
Departamento de Inmunologia, Microbiologia y Parasitología, Facultad de Medicina y Odontologia, Universidad del Pais Vasco, Bilbao, Spain. oipposaj@lg.ehu.es
Hernando
F L
FL
Moragues
M D
MD
Barea
P L
PL
Gerloni
M
M
Conti
S
S
Fisicaro
P
P
Cantelli
C
C
Polonelli
L
L
D000818:Animals; D000908:Antibodies, Fungal; D000946:Antigens, Fungal / Q000378:metabolism; D002176:Candida albicans / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity*; D002181:Candidiasis, Vulvovaginal / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007071:Immunoglobulin A, Secretory; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D007071:Immunoglobulin A, Secretory; D008562:Membrane Glycoproteins; C025363:mannoproteins