mRNA stabilization in continuous flow translation system.
In contrast with standard in vitro translation systems, where 1 to 2 copies of polypeptide per mRNA molecule are produced, the continuous flow cell-free translation system is able to synthesize hundreds of polypeptide molecules per one mRNA molecule. Our investigations have shown that the poor yield obtained in the standard analytical system is due to rapid mRNA decay as opposed feedback inhibition by low molecular weight translation by products. In contrast, continuous flow system was found to stabilize mRNA for up to two-three days. RNAse activity can not be removed from wheat germ extract unless mRNA is added and compartmentalization of the translational machinery occurs.
Alexandrov
A
A
Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
Kolosova
I
I
Kolosov
M
M
D002474:Cell-Free System; D004355:Drug Stability; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger