CMP-NeuAc:(NeuAc alpha 2-->8)n (colominic acid) sialyltransferase activity in rat brain and in tumour cells that express polysialic acid on neural cell adhesion molecules.
A method for the assay of CMP-NeuAc:(NeuAc alpha 2-->8)n (colominic acid) sialyltransferase activity was developed. Using a 1-day-old rat brain membrane fraction as an enzyme preparation optimal activity was obtained at pH 6.5, 0.3% Triton X-100, and 5 mM MnCl2. However, no absolute cation requirement was found as EDTA only partially inhibited the activity. Within a concentration range of 0.3-3 mg colominic acid (which consists of a mixture of oligomers of alpha 2-->8-linked sialic acid) per 50 microliters a V of 0.61 nmol per mg protein h-1 was estimated while a half-maximal reaction velocity was obtained at a concentration of 1.75 mg per 50 microliters. High performance anion-exchange chromatography of the radioactive products formed in the reaction showed that sialic acid oligomers ranging in size from a degree of polymerization (DP) of 2 up to at least DP 9 could serve as acceptor substrates. Comparison of the acceptor properties of DP 3 and DP 6 showed that the larger oligomer was acted upon with a 10-fold higher efficiency. Periodate oxidation of the products followed by reduction and hydrolysis yielded the C7 analogue of NeuAc as the only radioactive product, indicating that under the conditions of the assay only a single sialic acid residue was introduced into the acceptor molecules. Using the assay it appeared that in rat brain the activity of this sialyltransferase decreased six-fold during postnatal development to the adult stage. The assay method was also applied to lysates of several neuroblastoma and small cell lung tumour cell lines, which differ in the expression of polysialic acid as well as of the neural cell adhesion molecule NCAM, a major carrier of this polymer. Activity of the sialyltransferase appeared to be correlated with the expression of polysialic acid present on NCAM. These results indicate that this sialyltransferase might function in the process of poly-sialylation.
Easton
E W
EW
Department of Medical Chemistry, Vrije Universiteit, Amsterdam, Netherlands.
Schiphorst
W E
WE
Koeleman
C A
CA
Michalides
R J
RJ
Van Den Eijnden
D H
DH
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D018288:Carcinoma, Small Cell / Q000201:enzymology*; D002460:Cell Line; D007700:Kinetics; D008175:Lung Neoplasms / Q000201:enzymology*; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry*; D009447:Neuroblastoma / Q000201:enzymology*; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*; D012799:Sialyltransferases / Q000032:analysis*; D014407:Tumor Cells, Cultured
D019006:Neural Cell Adhesion Molecules; D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; C014420:colominic acid; C101784:CMP-NeuAc-(NeuAc-alpha-2-8)n sialyltransferase; D012799:Sialyltransferases