title: Apolipoprotein E is highly susceptible to oxidation by myeloperoxidase, an enzyme present in the brain.
abstract: Apolipoprotein E, the most common apolipoprotein found in the brain, is linked to several pathologies like Alzheimer's disease. Apolipoprotein E directly binds to beta-amyloid with a strong affinity. Myeloperoxidase, a protein secreted by neutrophils and involved in the inflammatory process, is also present in the brain. In vitro myeloperoxidase oxidation of recombinant human apolipoprotein E leads to fragmentation of the protein with low concentrations of hydrogen peroxide and polymerization with higher concentrations. Comparison with bovine serum albumin shows a higher susceptibility of apolipoprotein E to myeloperoxidase oxidation, which may have importance in the Alzheimer's disease process.
authors: URA CNRS 597, Centre du médicament, Nancy, France.
authors: C
authors: C
authors: Jolivalt
authors: B
authors: B
authors: Leininger-Muller
authors: R
authors: R
authors: Drozdz
authors: J W
authors: JW
authors: Naskalski
authors: G
authors: G
authors: Siest
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D010659:Phenylhydrazines; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D001057:Apolipoproteins E; D010659:Phenylhydrazines; D011994:Recombinant Proteins; C004787:2,4-dinitrophenylhydrazine; D009195:Peroxidase