Reinsemination of one-day-old oocytes by use of intracytoplasmic sperm injection.
OBJECTIVE
To evaluate the possible advantages of reinseminating oocytes by use of intracytoplasmic sperm injection (ICSI).


METHODS
Clinical study.


METHODS
In vitro fertilization unit with research facilities.


METHODS
Fifty-seven couples who were part of our regular IVF program.


METHODS
Nonfertilized oocytes from IVF cycles with no or very low fertilization were microinjected with spermatozoa approximately 25 hours after oocyte pick-up.


METHODS
Fertilization and pregnancy rates.


RESULTS
A mean fertilization rate of 46.5% was obtained when reinseminating the oocytes on day 2 using the ICSI procedure. Of 57 cycles with completely or almost completely failed fertilization, 29 patients received ET after reinsemination by ICSI. Two of these transfers resulted in pregnancies (6.9% per ET) and two healthy babies were born.


CONCLUSIONS
Despite this relative success, considering both the extra work involved and the potential genetic risk, it is doubtful whether ICSI on day 2 should be recommended as a routine procedure. For training and research purposes, however, this approach can be of value.
Lundin
K
K
Department of Obstetrics and Gynecology, University of Göteborg, Sweden.
Sjögren
A
A
Hamberger
L
L
D016922:Cellular Senescence; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007267:Injections; D007315:Insemination, Artificial; D008297:Male; D008846:Micromanipulation; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D013094:Spermatozoa; D016896:Treatment Outcome