Antibody penetration of tumor GS-7 xenografts in nude mice: a model for mucinous adenocarcinoma of the colon.
A new cell line derived from a human adenocarcinoma of the colon, GS-7, was propagated as a s.c. tumor in nude mice. This tumor histologically is a mucinous adenocarcinoma (also designated mucoid or colloid) with characteristic large mucin pools that are not lined by an epithelial layer but may contain scattered, randomly distributed cancer cells. Ten to 20% of human colorectal adenocarcinomas are of this histological type, but rapidly growing xenografts with this histology have been rarely used experimentally. This tumor, therefore, constitutes a useful model for similar human tumors. The mucin pools contain large amounts of carcinoembryonic antigen and tumor-associated glycoprotein 72, and the cells express epithelial glycoprotein 2 on their surface. The ability of antibodies injected i.v. to penetrate this tumor was investigated, using both biotinylated and radioiodinated antibodies (Abs). The results demonstrate that Abs can effectively penetrate the mucin pools, and that large amounts of Ab can localize there. This tumor type may have advantages as a target for certain forms of experimental immunotherapy.
Blumenthal
R D
RD
Garden State Cancer Center at the Center for Molecular Medicine and Immunology, Newark, New Jersey 07103, USA.
Stein
R
R
Sharkey
R M
RM
Goldenberg
D M
DM
Ong
G L
GL
Klein
K M
KM
Mattes
M J
MJ
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D001710:Biotin / Q000493:pharmacokinetics; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D000922:Immunotoxins / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D016499:Radioimmunotherapy; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000906:Antibodies; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D006023:Glycoproteins; D000922:Immunotoxins; D007457:Iodine Radioisotopes; C047052:tumor-associated antigen 72; D001710:Biotin