Identification of a membrane-associated receptor for transforming growth factor type E.
We have identified the receptor for epithelial type transforming growth factor (TGFe). TGFe, a member of the epithelin/granulin family of proteins, is present primarily in tissues of epithelial origin. It is a powerful mitogen for epithelial and fibroblastic cells. TGFe, iodinated using an immobilized glucose oxidase-lactoperoxidase method, was chemically crosslinked to receptors on membranes isolated from SW-13 adrenal carcinoma cells by the crosslinker disuccinimidyl suberate (DSS). The receptor appears to be a protein which migrates at an apparent molecular weight of approximately 170-175 kDa under reducing and nonreducing conditions in SDS-polyacrylamide gels.
Parnell
P G
PG
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, 30602-7388, USA.
Carter
B J
BJ
Halper
J
J
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D004848:Epithelium / Q000737:chemistry; D008970:Molecular Weight; D017978:Receptors, Growth Factor / Q000032:analysis*; D015291:Transforming Growth Factors / Q000378:metabolism*
D017978:Receptors, Growth Factor; D015291:Transforming Growth Factors