A new generation of the IMAGIC image processing system.
One of the aims of modern microscopy is to quantify two-, three-, or even four-dimensional phenomena in biology, medicine, and material sciences. The requirements imposed on software by such data processing are exemplified by the design considerations of the IMAGIC-5 software system. This system includes facilities for multivariate statistical analysis of large data sets, for correlation averaging of two-dimensional crystals, and for three-dimensional reconstruction of macromolecular structures. The molecules may be arranged as two-dimensional crystals, as helices, or as single particles with arbitrary pointgroup symmetry. IMAGIC's novel angular reconstitution approach allows for the rapid determination of three-dimensional structures of uncrystallized molecules to high resolution. The general organization, user interaction strategy, file structure, and extendibility of IMAGIC are discussed and illustrated with some practical examples.
van Heel
M
M
Fritz Haber Institute of the Max Planck Society, Berlin, Germany.
Harauz
G
G
Orlova
E V
EV
Schmidt
R
R
Schatz
M
M
D000818:Animals; D001435:Bacteriophages / Q000648:ultrastructure; D003198:Computer Simulation; D004926:Escherichia coli / Q000648:ultrastructure; D006454:Hemoglobins / Q000648:ultrastructure; D008854:Microscopy, Electron; D015999:Multivariate Analysis; D012270:Ribosomes / Q000648:ultrastructure; D012908:Snails; D012984:Software; D014764:Viral Proteins / Q000737:chemistry / Q000648:ultrastructure
D006454:Hemoglobins; D014764:Viral Proteins