Regulation of glibenclamide receptors in cultured chick cardiomyocytes.
We found that the expression of sulphonylurea receptors which control ATP-dependent potassium channels was up-regulated both by a receptor agonist and by potassium channel activators. Sulphonylurea receptors detected on cell membranes of cultured chick cardiomyocytes by [3H]glibenclamide were increased 2.8-fold following growth of the cells for 1-2 days in the presence of 30 nM glibenclamide. [3H]glibenclamide binding was also increased approximately 2-fold following growth of the cells in the presence of the ATP-dependent potassium channel activators, pinacidil, minoxidil and diazoxide, but was diminished to 75% of control following treatment with depolarizing concentrations of KCl.
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Department of Biochemical Pharmacology, State University of New York at Buffalo 14260, USA.
Hegde
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Halvorsen
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D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005905:Glyburide / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
D007004:Hypoglycemic Agents; D015221:Potassium Channels; D011955:Receptors, Drug; C067662:glibenclamide receptor; D005905:Glyburide