title: Localization of TIMP-3 mRNA expression to the retinal pigment epithelium.
abstract: OBJECTIVE
  To evaluate ocular sites of expression of the tissue inhibitor of metalloproteinases-3 gene (TIMP-3).
  
  
  METHODS
  In situ hybridization was performed on frozen sections of albino mouse eyes using riboprobes generated to the 3' untranslated region of TIMP-3.
  
  
  RESULTS
  TIMP-3 mRNA expression was detected strongly in the retinal pigment epithelium (RPE) and to a minor extent in the ciliary epithelium, but not at any other site within the eye.
  
  
  CONCLUSIONS
  Expression of TIMP-3 in the RPE is consistent with the recent demonstration of TIMP-3 mutations in patients with Sorsby's fundus dystrophy, a condition marked by the early onset of choroidal neovascularization in the macula. Unlike many of the recently described genes that cause human retinal disease, TIMP-3 is preferentially expressed in the RPE of the normal eye, as opposed to the photoreceptors.
authors: Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287-9289, USA.
authors: N G
authors: NG
authors: Della
authors: P A
authors: PA
authors: Campochiaro
authors: D J
authors: DJ
authors: Zack
mesh_terms: D000417:Albinism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D019717:Tissue Inhibitor of Metalloproteinase-3; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D019717:Tissue Inhibitor of Metalloproteinase-3