Retinoic acid enhances expression of bone morphogenetic protein-2 in human adenocarcinoma cell line (HSG-S8).
Expression of bone morphogenetic protein (BMP)-2 mRNA was stimulated by retinoic acid in human adenocarcinoma cell line, HSG-S8, in a dose-dependent manner. Northern blot analysis demonstrated that retinoic acid most strongly increased the level of BMP-2 mRNA 6 h after the treatment and the stimulatory effect was maintained at 48 h. The mature peptides of 16 and 18 kDa molecular masses of BMP-2 were also increased in the conditioned medium by the treatment of retinoic acid on western blotting. The proliferation of HSG-S8 cells was inhibited by retinoic acid, however, retinoic acid did not cause morphological change showing cellular differentiation. 1 alpha, 25(OH)2D3, like retinoic acid, clearly increased the mRNA level of BMP-2, whereas dibuthryl cyclic AMP remarkably diminished it, and bromodeoxyuridine had no effect on the expression of BMP-2 mRNA.
Hatakeyama
S
S
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Morioka, Japan.
Ohara-Nemoto
Y
Y
Kyakumoto
S
S
Satoh
M
M
D000230:Adenocarcinoma / Q000378:metabolism*; D015153:Blotting, Western; D019485:Bone Morphogenetic Proteins; D003994:Bucladesine / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D006801:Humans; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects
D019485:Bone Morphogenetic Proteins; D016895:Culture Media, Serum-Free; D012333:RNA, Messenger; D014316:Tritium; D014212:Tretinoin; D003994:Bucladesine; D002117:Calcitriol; D013936:Thymidine