Differential effects of chylomicron remnants derived from corn oil or palm oil on bile acid synthesis and very low density lipoprotein secretion in cultured rat hepatocytes.
The effects of chylomicron remnants derived from corn oil (rich in n-6 polyunsaturated fatty acids) and palm oil (rich in long chain saturated fatty acids) on bile acid synthesis and very low density lipoprotein secretion in cultured rat hepatocytes were studied. Incubation of the cells with corn oil remnants led to increased bile acid production, while the secretion of lipid in very low density lipoprotein remained unchanged. In contrast, addition of palm oil remnants to the medium did not affect bile acid synthesis, but resulted in the secretion of cholesterol-rich very low density lipoprotein. These findings show that chylomicron remnants of different fatty acid composition have differential effects on cholesterol metabolism in liver cells, and provide part of the explanation for the hyper- and hypocholesterolaemic effects of saturated and polyunsaturated fatty acids.
Bravo
E
E
Istituto Superiore di Sanita, Laboratory of Metabolism and Pathological Biochemistry, Rome, Italy.
Cantafora
A
A
Marinelli
T
T
Avella
M
M
Mayes
P A
PA
Botham
K M
KM
D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002478:Cells, Cultured; D019826:Cholic Acid; D002793:Cholic Acids / Q000096:biosynthesis; D002914:Chylomicrons / Q000097:blood* / Q000494:pharmacology*; D003314:Corn Oil; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D000073878:Palm Oil; D010938:Plant Oils; D051381:Rats; D017208:Rats, Wistar
D001647:Bile Acids and Salts; D002793:Cholic Acids; D002914:Chylomicrons; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D010938:Plant Oils; C004821:muricholic acid; D000073878:Palm Oil; D003314:Corn Oil; D019826:Cholic Acid