Characterization and linkage mapping of ten sheep microsatellite markers derived from a sheep x hamster cell hybrid.
A cosmid library has been constructed from a sheep x hamster cell hybrid containing sheep chromosome t1, rob (6;24). Clones containing sheep DNA were identified by hybridizing to a total sheep genomic DNA probe. Small fragments (< 500 bp) containing (AC)n microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining microsatellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line.
Lumsden
J M
JM
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Lord
E A
EA
Montgomery
G W
GW
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae / Q000235:genetics*; D015723:Gene Library; D008040:Genetic Linkage; D006822:Hybrid Cells; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*