Antimicrobial activity of chicken and turkey heterophil peptides CHP1, CHP2, THP1, and THP3.
Four avian heterophil antimicrobial cationic peptides (Chicken Heterophil Peptides 1 and 2, and Turkey Heterophil Peptides 1 and 3) were evaluated for in vitro microbicidal activity against selected avian pathogens and human pathogens which are harbored by birds. At concentrations of 16-2 micrograms/ml, all four avian peptides effected a greater than 90% reduction in the survival of Candida albicans, Salmonella enteriditis, and Campylobacter jejuni. None of the peptides, including the known antimicrobial peptide protamine (used as a positive control), were able to reduce the survival of Pasteurella multocida by 90% at the maximum peptide concentration (16 micrograms/ml) tested. At 16 micrograms/ml, the turkey peptide THP3 did not effect a 90% reduction in survival of Bordetella avium, Escherichia coli, or Salmonella typhimurium, while all of the other peptides tested were effective at this concentration or less. This peptide, THP3, does not share the same homologous amino acid sequence shared by the other three peptides. Under our experimental conditions, none of the peptides neutralized Infectious Bronchitis Virus, an enveloped coronavirus of chickens.
Evans
E W
EW
Department of Veterinary Pathology, University of Georgia, Athens, USA. EvansE@svm.vetmed.wisc.edu
Beach
F G
FG
Moore
K M
KM
Jackwood
M W
MW
Glisson
J R
JR
Harmon
B G
BG
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D030161:Avian Proteins; D001884:Bordetella / Q000187:drug effects; D016123:Campylobacter jejuni / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D002645:Chickens; D023082:Defensins; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D011506:Proteins / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D014422:Turkeys
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D030161:Avian Proteins; D023082:Defensins; D011506:Proteins; C086732:gallinacin 1 protein, chicken