[In vitro study of the bacteriostatic and bactericidal activity of meropenem against strains of Pseudomonas aeruginosa multiresistant to antibiotics].
Meropenem is a new dehydropeptidase stable carbapenem which has an in vitro bacteriostatic activity superior to imipenem against Pseudomonas aeruginosa. Minimal inhibitory concentrations of meropenem (MEM) and imipenem (IPM) were determined on twelve strains: two were genetically characterized reference strains and ten isolated from patients. Killing curves were performed for MEM used at 1,2 and 4 fold the MIC and 32 mg/l; survivors were counted at baseline and after 5 and 24 hours of incubation by spiral plating. Five strains were susceptible to MEM and IPM; five were intermediate and two resistant to MEM; these seven strains were resistant to IPM. A weak bactericidal effect was obtained after 5 h at each concentration. Only one strain achieved a 3 log 10 reduction after 5 h at 2 x MIC. A regrowth of all the strains tested was observed after 24 h.
Delisle
F
F
Laboratoire Central de Microbiologie, CHU Saint-Antoine, Paris, France.
Lesage
D
D
Barakett
V
V
Richard
G
G
Petit
J C
JC
D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology*; D066298:In Vitro Techniques; D000077731:Meropenem; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013845:Thienamycins / Q000494:pharmacology*
D013845:Thienamycins; D015378:Imipenem; D000077731:Meropenem
Etude in vitro de l'activité bactériostatique et bactéricide du méropénéme sur des souches de Pseudomonas aeruginosa multirésistantes aux antibiotiques.