title: Isolation and modulation of growth of a colonization-impaired strain of Enterobacter cloacae in cucumber spermosphere.
abstract: Enterobacter cloacae A-46 was isolated for use in an environmental containment strategy for genetically modified derivative strains with enhanced biocontrol activity. The population of E. cloacae A-46, a transposon mutant of the plant-beneficial bacterium E. cloacae 501R3, increased 10-fold (significant increase at P < or = 0.05) in cucumber spermosphere when applied to cucumber seeds along with casamino acids. In contrast, strain A-46 was incapable of proliferation in cucumber spermosphere in the absence of casamino acids. Populations of strain A-46 also failed to increase in corn, cowpea, sunflower, and wheat spermospheres in the absence of casamino acids, while populations of strain 501R3 increased 3162-, 512-, 1698-, and 93-fold, respectively. In addition, the persistence of strain A-46 in corn, cucumber, and sunflower rhizospheres and in natural soil was greatly reduced compared with the parental strain 501R3.
authors: Biocontrol of Plant Diseases Laboratory, U.S. Department of Agriculture, Beltsville, MD 20705, U.S.A.
authors: D P
authors: DP
authors: Roberts
authors: A M
authors: AM
authors: Marty
authors: P D
authors: PD
authors: Dery
authors: J S
authors: JS
authors: Hartung
mesh_terms: D018553:Cucumis sativus / Q000382:microbiology*; D003470:Culture Media; D016972:Enterobacter cloacae / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016254:Mutagenesis, Insertional; D010944:Plants / Q000382:microbiology; D012639:Seeds; D013045:Species Specificity; D013997:Time Factors
chemical_list: D003470:Culture Media