Isolation and characterization of a kettin-like protein from crayfish claw muscle.
A 540 kDa protein was isolated from crayfish claw muscle (closer). The secondary structure mainly consisted of beta-sheet (70%). The rotary shadowed images were long filaments, 300-360 nm long. It is localized in the sides of the Z-lines extending to the I band and elongatable upon stretch of muscle. Immunological crossreactivities strongly suggested that this protein corresponds to kettin (500-700 kDa) of insect striated muscle. In view of molecular shape and secondary structure, and immunological crossreactivities, it is suggested that this kettin-like protein belongs to connectin/titin family of striated muscle.
Maki
S
S
Department of Biology, Faculty of Science, Chiba University, Japan.
Ohtani
Y
Y
Kimura
S
S
Maruyama
K
K
D000199:Actins / Q000378:metabolism; D000818:Animals; D003400:Astacoidea / Q000737:chemistry*; D002942:Circular Dichroism; D064211:Connectin; D029721:Drosophila Proteins; D005455:Fluorescent Antibody Technique; D006724:Hoof and Claw / Q000737:chemistry / Q000648:ultrastructure; D015151:Immunoblotting; D007301:Insect Hormones / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D019476:Insect Proteins; D007313:Insecta; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000648:ultrastructure; D011817:Rabbits
D000199:Actins; D064211:Connectin; D029721:Drosophila Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D009124:Muscle Proteins; C577347:sls protein, Drosophila