Activation of phospholipase D in cultured human amnion cells.
The regulation of phospholipase D (PLD) activity in the human amniotic membrane was examined using primary cultures of amnion cells. Cultured amnion cells were labelled with [3H]oleic acid, and PLD activity was determined as the amount of [3H]phosphatidylethanol (PEt) produced during incubation in the presence of 0.1% ethanol. PLD activity in cultured amnion cells was activated by addition of arginine vasopressin and oxytocin. PLD activity was also stimulated by treatment was arachidonic acid, the product of phospholipase A2 (PLA2), and phospholipase C (PLC). These results indicate that PLD in amnion cells is activated by substances present in amniotic fluid, and that cross-talk between phospholipases A2, C and D may occur in amnion cells.
Inamori
K
K
Department of Obstetrics and Gynaecology, Kyoto University Faculty of Medicine, Japan.
Sagawa
N
N
Hasegawa
M
M
Itoh
H
H
Yano
J
J
Mori
T
T
D000650:Amnion / Q000201:enzymology*; D016718:Arachidonic Acid / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D020404:Glycerophospholipids; D006801:Humans; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010121:Oxytocin / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010739:Phospholipase D / Q000378:metabolism*; D011247:Pregnancy; D010738:Type C Phospholipases / Q000494:pharmacology
D020404:Glycerophospholipids; D009829:Oleic Acids; D010712:Phosphatidic Acids; C051521:phosphatidylethanol; D001127:Arginine Vasopressin; D016718:Arachidonic Acid; D019301:Oleic Acid; D010121:Oxytocin; D010738:Type C Phospholipases; D010739:Phospholipase D