Protective effect of cystathionine on acute gastric mucosal injury induced by ischemia-reperfusion in rats.
We studied the protective effect of cystathionine on acute gastric mucosal injury induced by ischemia-reperfusion in rats. Under pentobarbital anesthesia, the celiac artery was clamped for 30 min and reperfused. Sixty minutes after the reperfusion, the total area of erosions and thiobarbituric acid-reactive substances in the stomach, as an index of lipid peroxidation, were measured and compared between control and cystathionine-treated groups. Intraperitoneal administration of cystathionine (1-20 mg/kg) 10 min before the ischemia significantly reduced both the total area of erosions and the level of thiobarbituric acid-reactive substances. When cystathionine (10 mg/kg) was administered orally, the significant reductions in the total area of erosions and level of thiobarbituric acid-reactive substances were also observed. There was a good correlation between the total area of erosions and the level of thiobarbituric acid-reactive substances. Cystathionine did not affect blood flow during ischemia-reperfusion. These results indicate that the protective effect of cystathionine on acute gastric mucosal injury induced by ischemia-reperfusion may be due to the scavenging action against superoxide radicals in vivo.
Wada
K
K
Department of Clinical Pharmacology, Faculty of Medicine, Tottori University, Yonago, Japan.
Kamisaki
Y
Y
Kitano
M
M
Nakamoto
K
K
Itoh
T
T
D000818:Animals; D003540:Cystathionine / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000187:drug effects; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
D016166:Free Radical Scavengers; D003540:Cystathionine