Analysis of benzoylecgonine in dried blood spots by liquid chromatography--atmospheric pressure chemical ionization tandem mass spectrometry.
Residual samples from blood spots (i.e., whole blood spotted onto filter paper) are a useful source for epidemiological screening studies involving newborns. However, the small volume of blood available from residual blood spots complicates the assay. A method for analyzing benzoylecgonine (BZE; the primary metabolite of cocaine) in blood spots, in which the blood spot is eluted with aqueous ammonium acetate-methanol containing N-methyl trideuterated-BZE as an internal standard, followed by high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry using multiple reaction monitoring, has been developed. This approach provides a rapid, direct, sensitive (limit of detection, approximately 2 ng/mL, based on a 12-microL sample size), and highly specific means of determining BZE concentrations in blood spots. We have applied this method for confirmatory analyses in a large epidemiological study of the prevalence of cocaine use during late pregnancy.
Sosnoff
C S
CS
Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, U.S. Public Health Service, Atlanta, GA 30333, USA.
Ann
Q
Q
Bernert
J T
JT
Powell
M K
MK
Miller
B B
BB
Henderson
L O
LO
Hannon
W H
WH
Fernhoff
P
P
Sampson
E J
EJ
D000085:Acetates / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives* / Q000097:blood; D003903:Deuterium; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D007553:Isotope Labeling; D008431:Maternal-Fetal Exchange; D000432:Methanol / Q000737:chemistry; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000453:epidemiology; D011863:Radioimmunoassay; D012015:Reference Standards; D012680:Sensitivity and Specificity; D019966:Substance-Related Disorders / Q000097:blood / Q000453:epidemiology
D000085:Acetates; C005618:benzoylecgonine; D003903:Deuterium; D003042:Cocaine; C018824:ammonium acetate; D000432:Methanol