Adhesion of K88ab to guinea pig erythrocytes: effect on membrane enzyme activities.
Nontoxigenic Escherichia coli strains bearing K88 fimbriae have been associated with diarrhea in piglets. We have used guinea pig erythrocytes as a model of the host cell to study the cellular alterations after adherence of purified K88ab fimbriae. Although Mg2+-dependent ATPase was inhibited (up to 61%), Na/K ATPase was not. Metabolic enzymes were not significantly affected.
Caloca
M J
MJ
Departmento de Bioquimica y Biologia Molecular, Universidad de Leon, Spain.
Soler
J
J
Suárez
S
S
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000378:metabolism*; D001422:Bacterial Adhesion; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006168:Guinea Pigs; D010957:Plasmids; D011485:Protein Binding; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase