Immunochemical methods for quantification of apolipoprotein A-IV.
Several methods are available for the immunoassay of apoA-IV levels in plasma, or lipoproteins. The method of choice depends on the question being asked. If sensitivity is not a major determinant, simple immunoelectrophoresis is probably sufficient. To determine apoA-IV levels in plasma or lipoprotein fractions, either radioimmunoassay or a competitive ELISA is indicated. The competitive ELISA described above, however, offers sensitivity as well as rapidity and case of performance. When very low levels of apoA-IV are present (such as those produced by cultured cells), the higher sensitivity of the sandwich ELISA may be required.
Fidge
N H
NH
Lipoprotein-Atherosclerosis Unit, Baker Medical Research Institute, Melbourne, Victoria, Australia.
D000818:Animals; D001054:Apolipoproteins A / Q000032:analysis* / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002914:Chylomicrons / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007122:Immunoelectrophoresis; D050356:Lipid Metabolism; D011863:Radioimmunoassay; D051381:Rats
D001054:Apolipoproteins A; D002914:Chylomicrons; D007106:Immune Sera; C022205:apolipoprotein A-IV