Acetate represents a major product of heptanoate and octanoate beta-oxidation in hepatocytes isolated from neonatal piglets.
An experiment was conducted to explore the nature of the radiolabel distribution in acid-soluble products (ASPs) resulting from the oxidation of [1-14C]C7:0 or C8:0 by isolated piglet hepatocytes. The differences between odd and even chain-length and the impacts of valproate and malonate upon the rate of beta-oxidation and ASP characteristics were tested. A minor amount of fatty acid carboxyl carbon (< or = 10% of organic acids identified by radio-HPLC) accumulated in ketone bodies regardless of chain-length or inhibitor used. In all cases, acetate represented the major reservoir of carboxyl carbon, accounting for 60-70% of radiolabel in identified organic acids. Cells given [1-14C]C7:0 accumulated 85% more carboxyl carbon in Krebs cycle intermediates when compared with C8:0, while accumulation in acetate was unaffected. The results are consistent with the hypothesis that anaplerosis from odd-carbon fatty acids affects the oxidative fate of fatty acid carbon. The piglet appears unique in that non-ketogenic routes of fatty acid carbon flow (i.e. acetogenesis) predominate in the liver of this species.
Lin
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Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA.
Adams
S H
SH
Odle
J
J
D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002210:Caprylates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002331:Carnitine / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000378:metabolism; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism; D006538:Heptanoic Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008314:Malonates / Q000494:pharmacology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013552:Swine; D014635:Valproic Acid / Q000494:pharmacology
D000085:Acetates; D002210:Caprylates; D005227:Fatty Acids; D006538:Heptanoic Acids; D008314:Malonates; D002245:Carbon Dioxide; D014635:Valproic Acid; D000105:Acetyl Coenzyme A; C030290:malonic acid; C031492:octanoic acid; D002331:Carnitine; D003065:Coenzyme A