Simple method for detection of MYH11 DNA rearrangements in patients with inv(16)(p13q22) and acute myeloid leukemia.
The pericentric inversion on chromosome 16 [inv(16)(p13q22)] and related t(16;16)(p13;q22) are recurrent aberrations associated with acute myeloid leukemia (AML) M4 Eo. Both abberations result in a fusion of the core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11). A selected genomic 6.9-kb BamHl probe detects MYH11 DNA rearrangements in 18 of 19 inv(16)/t(16;16) patients tested using HindIII digested DNA. The rearranged fragments were not detectable after remission in two cases tested, while they were present after relapse in one of these two cases tested.
van der Reijden
B A
BA
Department of Human Genetics, Leiden University, Sylvius Laboratories, The Netherlands.
Martinet
D
D
Dauwerse
J G
JG
Giles
R H
RH
Wessels
J W
JW
Beverstock
G C
GC
Smit
B
B
Mühlematter
D
D
Jotterand Bellomo
M
M
Gabert
J
J
Lafage-Pochitaloff
M
M
Reiffers
J
J
Bilhou-Nabera
C
C
van Ommen
G J
GJ
Hagemeijer
A
A
Breuning
M H
MH
D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16; D004273:DNA, Neoplasm / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis / Q000235:genetics; D014178:Translocation, Genetic
C095514:CBFbeta-MYH11 fusion protein; D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; D018995:Myosin Heavy Chains