title: Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells.
abstract: The formation of the mitotic spindle is an essential prerequisite for successful mitosis. The dramatic changes in the level of microtubule (Mt) nucleation at the centrosomes and Mt dynamics that occur in prophase are presumed to be initiated through the activity of cdc2/cyclin B. Here we present data that the cdc25B isoform functions to activate the cytoplasmic pool of cdc2/cyclin B responsible for these events. In contrast to cdc25C, cdc25B is present at low levels in HeLa cells during interphase, but sharply increases in prophase, when cdc25B accumulation in the cytoplasm correlates with prophase spindle formation. Overexpression of wild type and dominant negative mutants of cdc25B and cdc25C shows that prophase Mt nucleation is a consequence of cytoplasmic cdc25B activity, and that cdc25C regulates nuclear G2/M events. Our data also suggest that the functional status of the centrosome can regulate nuclear mitotic events.
authors: Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
authors: B G
authors: BG
authors: Gabrielli
authors: C P
authors: CP
authors: De Souza
authors: I D
authors: ID
authors: Tonks
authors: J M
authors: JM
authors: Clark
authors: N K
authors: NK
authors: Hayward
authors: K A
authors: KA
authors: Ellem
mesh_terms: D000911:Antibodies, Monoclonal; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D018385:Centrosome / Q000648:ultrastructure*; D003593:Cytoplasm / Q000201:enzymology*; D016195:G2 Phase / Q000502:physiology*; D005799:Genes, Dominant; D006367:HeLa Cells; D006801:Humans; D008677:Metaphase / Q000502:physiology*; D008870:Microtubules / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011418:Prophase / Q000502:physiology; D008941:Spindle Apparatus; D020687:cdc25 Phosphatases
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D010749:Phosphoprotein Phosphatases; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases