Vaccination of cattle with bovine papillomavirus type 4 L2 elicits the production of virus-neutralizing antibodies.
Prophylactic vaccination of cattle with the N terminus (L2a, aa 11-200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the alimentary canal. To investigate the mechanisms underlying protection from viral infection, sera from vaccinated animals were analysed in neutralization assays both in the nude mouse xenograft system and in cattle. BPV-4 retained its infectivity when incubated with preimmune cattle sera, whereas, when incubated with immune sera from animals vaccinated with either whole L2 or its N terminus L2a, its infectivity was greatly reduced, indicating that the immune sera had neutralizing activity against the virus. This activity could be abrogated by absorbing the immune sera with L2 or L2a, thus indicating that virus neutralization was due to the presence in the immune sera of anti-L2 antibodies.
Gaukroger
J M
JM
Department of Veterinary Pathology, Glasgow, UK.
Chandrachud
L M
LM
O'Neil
B W
BW
Grindlay
G J
GJ
Knowles
G
G
Campo
M S
MS
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D052697:Bovine papillomavirus 4; D002213:Capsid / Q000008:administration & dosage / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D005260:Female; D051379:Mice; D008819:Mice, Nude; D009500:Neutralization Tests; D030361:Papillomavirus Infections / Q000276:immunology / Q000517:prevention & control*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014779:Virus Replication
D000914:Antibodies, Viral; D036022:Capsid Proteins; C085218:L2 protein, Bovine papillomavirus; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines