Differential expression of CD22 (Lyb8) on murine B cells.
Previous studies have established the distribution, biochemistry and functional attributes of human CD22, a B cell-restricted glycoprotein. Recently, molecular cloning of the murine CD22 equivalent revealed this molecule to be the same as the previously described Lyb8 alloantigen. Using the anti-Lyb8 mAb Cy34.1.2, the present report documents the expression patterns of CD22 within the murine B cell compartment. The results demonstrate that in the bone marrow, murine CD22 is absent on the surface of pro-B cells, pre-B cells and newly emerging IgM+ B cells. CD22 is present at a low density on immature IgMhi B cells and fully expressed on mature recirculating B cells. In the periphery, murine CD22 is expressed at mature levels on all B cell subsets including follicular, marginal zone, B1 and switched B cells. Further studies showed CD22 to be retained on activated murine B cells for extended periods. Finally, in combination with CD23 and heat stable antigen, CD22 can be used to delineate the immature splenic B cells, and distinguish them from follicular and marginal zone cells. Together, the results demonstrate murine CD22 to be a useful pan marker for all mature B cell subsets.
Erickson
L D
LD
Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA.
Tygrett
L T
LT
Bhatia
S K
SK
Grabstein
K H
KH
Waldschmidt
T J
TJ
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D051927:CD24 Antigen; D015815:Cell Adhesion Molecules; D005434:Flow Cytometry; D015322:Gene Rearrangement, B-Lymphocyte; D037102:Lectins; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism; D017455:Receptors, IgE / Q000096:biosynthesis; D051918:Sialic Acid Binding Ig-like Lectin 2; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D051927:CD24 Antigen; C497868:Cd22 protein, mouse; C497890:Cd24a protein, mouse; D015815:Cell Adhesion Molecules; D037102:Lectins; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D051918:Sialic Acid Binding Ig-like Lectin 2