Biopersistence of man-made vitreous fibers and crocidolite asbestos in the rat lung following inhalation.
This study investigated possible relationships between fiber bio-persistence in the lung and previously observed differences in pulmonary toxicity between asbestos and man-made vitreous fibers (MMVF) following inhalation exposure. Fischer 344/N rats were exposed nose only, 6 hr/day for 5 days to 30 mg/m3 MMVF (two fiberglass compositions, rock wool, or slag wool) or to 10 mg/m3 crocidolite asbestos. At eight time points up to 1 year postexposure, lung fiber burdens were analyzed for number/lung and bivariate dimensions using scanning electron microscopy (SEM) and for chemical composition using SEM energy dispersive spectroscopy. After 365 days, > 95% of long (> 20 microns) MMVFs had disappeared from the lung compared to only 17% of long crocidolite fibers. Longer MMVFs disappeared more rapidly than short MMVFs, suggesting that long fibers were dissolving or breaking. Mean diameters and lengths of the MMVFs decreased with time, while the mean diameter of crocidolite remained unchanged and its mean length showed an apparent increase, probably related to macrophage-mediated clearance of short fibers. Leaching of oxides occurred in the fibrous glasses and slag wool and correlated with morphological changes in the fibers over time. No chemical or morphological changes were observed in crocidolite fibers. These changes in MMVF number, chemistry, and morphology over time in lung tissue compared to crocidolite asbestos demonstrate the relatively low biological persistence of some MMVFs in the lung and may explain why these MMVFs are not tumorigenic in rats, even after chronic exposure at high concentrations.
Hesterberg
T W
TW
Schuller International, Inc., Littleton, Colorado 80162-5005, USA.
Miiller
W C
WC
Musselman
R P
RP
Kamstrup
O
O
Hamilton
R D
RD
Thevenaz
P
P
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D017638:Asbestos, Crocidolite / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D001665:Binding Sites; D002273:Carcinogens / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D004577:Electron Probe Microanalysis; D005898:Glass / Q000737:chemistry; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011916:Rats, Inbred F344
D000336:Aerosols; D002273:Carcinogens; C009753:fiberglass; D017638:Asbestos, Crocidolite