Investigation of newly synthesized peptides by capillary zone electrophoresis/electrospray mass spectrometry.
A series of newly synthesized peptides (M(r) = 1600-2250 Da), corresponding to portions of the extracellular domain of human granulocyte-macrophage colony stimulating factor receptor alpha subunit have been examined by capillary zone electrophoresis/electrospray-mass spectrometry (CZE/ES-MS). The separation efficiency of CZE combined with the specificity of mass spectrometry allowed rapid and reliable identification of the target peptides and a number of associated side products. In solid-phase synthesis of peptides and small proteins such side products are inevitable, therefore the use of CZE and/or high-performance liquid chromatography combined with mass spectrometry is gaining a central role in such synthesis procedures.
Hamdan
M
M
Glaxo Wellcome, Medicines Research Centre, Verona, Italy.
Curcuruto
O
O
Vigano
S
S
Rovero
P
P
D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor