Detection of anti-HIV-1 IgG antibodies in whole saliva by GACELISA and Western blot assays.
The present study, based on 158 HIV seropositives and 167 HIV seronegatives, demonstrates that saliva collected with the Omni-SAL device and tested with GACELISA (an IgG antibody capture ELISA) is an effective non-invasive alternative to serum for anti-HIV IgG antibody screening. The study also shows that a conventional serum Western blot kit can be used, with slight modifications, for confirmatory testing of saliva specimens. Collecting saliva with the Omni-SAL device had a very good acceptance rate among Tanzanian subjects, and although this diagnostic method is not yet known by the general public, 65% of the study participants preferred to give saliva instead of blood for HIV testing.
Matee
M I
MI
Department of Microbiology and Immunology, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania.
Lyamuya
E F
EF
Simon
E
E
Mbena
E C
EC
Kagoma
C
C
Samaranayake
L P
LP
Scheutz
F
F
D000328:Adult; D015153:Blotting, Western / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015483:HIV Antibodies / Q000032:analysis*; D006679:HIV Seropositivity / Q000097:blood / Q000175:diagnosis; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D010342:Patient Acceptance of Health Care; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity; D013636:Tanzania
D015483:HIV Antibodies; D007074:Immunoglobulin G