Reduction of cerebellar GABAA responses by interleukin-1 (IL-1) through an indomethacin insensitive mechanism.
Recently, a role of IL-1 in the central nervous system has been described, principally a fever-inducing effect in the hypothalamus through a prostaglandin second messenger system. IL-1 has also been shown to potentiate gamma-aminobutyric acid (GABAA) responses in embryonic chick neurones. This study describes the investigation of the effect of IL-1 on GABAA responses within the in vitro rat cerebellar slice, a preparation containing intact neuronal circuitry. Stimulation of the area of passage of paralleled fibres produced a pure GABAA inhibition of the spontaneous firing of Purkinje cells. 5 and 10 ng/ml IL-1 produced a reduction in the duration of inhibition 10 min after beginning perfusion of IL-1. This effect reversed within 15 min of washing out the IL-1. 10 ng/ml IL-1 also reduced the effects of exogenously-applied GABA (0.1 mM) with the same time course. In the presence of 1 uM indomethacin, there was no change in the effect of the IL-1. It can therefore be concluded that the reduction in cerebellar GABAA responses by IL-1 is not mediated by the indomethacin-sensitive prostaglandin second messenger system.
Pringle
A K
AK
Department of Physiology and Pharmacology, University of Southhampton, U.K.
Gardner
C R
CR
Walker
R J
RJ
D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000502:physiology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004594:Electrophysiology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D011689:Purkinje Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D007375:Interleukin-1; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D007213:Indomethacin