[Alternation of intracellular Ca2+ caused by streptomycin in the isolated cochlear OHC].
Stained with fluorescence, the intracellular free calcium concentration (Ca2+) of the cochlear out hair cell (OHC) isolated from guinea pigs was measured using ACAS 570. Streptomycin caused a remarkable rise in Ca2+ and reached peak value in 60s, decreased gradually after lasting for 60s in that value. The Ca2+ fell back to primary level in another 100s and followed by a slight drop. The distribution of free calcium in the OHC was showed. The rise of intracellular free calcium in the OHC was assumed to be a period in the ototoxicity caused by aminoglycoside antibiotics.
Li
Y
Y
Zhujang Hospital, First Military Medical University, Guangzhou.
Huang
Y
Y
Luo
S
S
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D013307:Streptomycin / Q000633:toxicity*
D002118:Calcium; D013307:Streptomycin