Using electroporation and a slot cuvette to deliver plasmid DNA to insect embryos.
Microinjection is the method used almost exclusively to deliver DNA constructs to insect embryos while electroporation is commonly used for DNA delivery to bacteria, cell cultures and certain plant tissues. This communication describes a method using an easily constructed slot cuvette and the electroporation technique for transfer of DNA to insect embryos for possible use in developing methods for germline transformation. This method eliminates time-consuming individual embryo manipulation and thus far has been found to be adaptable for use on several types of insect embryos. Using this method, we show successful transfer of plasmid DNA to embryos of the corn earworm moth, Helicoverpa zea, and the house fly, Musca domestica.
Leopold
R A
RA
U.S. Department of Agriculture, ARS Biosciences Research Laboratory, Fargo, ND 58105, USA. rleopold@VM1.nodak.edu
Hughes
K J
KJ
DeVault
J D
JD
D000818:Animals; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D005818:Genetic Engineering; D006793:Houseflies / Q000196:embryology* / Q000235:genetics*; D009036:Moths / Q000196:embryology* / Q000235:genetics*; D010957:Plasmids / Q000008:administration & dosage*