Nucleotide sequence of exons 5 to 9 of the p53 tumour-suppressor gene of the donkey (Equus asinus).
The evolutionary conserved region of the equine homologue of the p53 gene from the donkey genome was PCR amplified and cloned. The 1380 bp fragment consisted of exons 5 to 9 and the intervening introns. The exonic and intronic DNA sequences showed a variable but high level of homology with previously published human sequences. The aminoacid sequences corresponding to the evolutionary conserved domains II, III, and V were identical to the human regions, whilst domain IV was 96% homologous.
Nasir
L
L
Department of Veterinary Medicine, University of Glasgow Veterinary School, Scotland, United Kingdom.
Reid
S W
SW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018397:Equidae / Q000235:genetics*; D005091:Exons; D016158:Genes, p53; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction