A new urea sensor based on combining the surface acoustic wave device with urease extracted from green soya bean and its application--determination of urea in human urine.
The urea sensor was prepared by combining a surface acoustic wave (SAW) device, in which a SAW resonator operating at 61 MHz and a pair of parallel electrodes were used in series, with urease extracted from green soya bean. The Michaelis constant and maximum reaction rate of the urease were estimated as 2.14 mM and 27.18 kHz min-1, respectively, at pH 7.0 and 25.0 degrees C. Influences of pH, temperature and effectors on the response properties of the SAW urea sensor were investigated. Recovery of the sensor ranged from 95 to 105% and the detection limit of urea was 1.0 micrograms ml-1 (1.7 x 10(-5) M). The proposed sensor has been successfully applied to the rapid determination of urea in human urine samples. The results are consistent with the reported values and also support the clinical diagnosis.
Liu
D
D
Department of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Chen
K
K
Ge
K
K
Nie
L
L
Yao
S
S
D000162:Acoustics / Q000295:instrumentation*; D015374:Biosensing Techniques; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012680:Sensitivity and Specificity; D012997:Solvents; D013025:Glycine max / Q000201:enzymology*; D013499:Surface Properties; D013696:Temperature; D014508:Urea / Q000652:urine*; D014510:Urease / Q000302:isolation & purification*
D012997:Solvents; D014508:Urea; D014510:Urease