Preliminary study on the molecular structure of 3' region of Duchenne muscular dystrophy (DMD) gene in Chinese.
Number and order of HindIII exon-containing fragments (Hd) at 3' region of DMD gene were studied systematically using 16 partly-overlapping cDNA subprobes which were produced from dystrophin cDNA 9-14 with each of 9 restriction endonucleases. There are 25 Hd fragments corresponding to cDNA 9-14 in DMD gene. Since then, the exact length and the new order of Hd fragments are established. A new 2.1 kb fragment (Hd 55) is revealed; a 5.2 kb fragment (formerly designated as Hd 59) is excluded and the existence of a controversial 3.2 kb fragment (Hd 64) is confirmed. Besides, three new exons were revealed by comparing the PvuII and the XbaI hybridization patterns with the HindIII hybridization patterns for these cDNA subprobes. It is concluded that there are at least 66 Hd fragments, or 79 exons in DMD gene basing on the discovery of three additional exons. The corresponding relationship between the 66 Hd fragments and the SfiI large scale physical map has been studied, and at least 17 Hd fragments or 19 exons were shown to be distributed in the last fragment (IJ fragment) of the SfiI map of DMD gene.
Yu
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L
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, China.
Zhao
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S
D018076:DNA, Complementary / Q000235:genetics; D016189:Dystrophin / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D009136:Muscular Dystrophies / Q000235:genetics*
D018076:DNA, Complementary; D016189:Dystrophin