Characterization of centchroman binding protein in plasma of rhesus monkey (Macaca mulatta).
Centchroman, a nonsteroidal antifertility agent was studied for its binding to monkey (Macaca mulatta) plasma proteins using charcoal adsorption and electrophoretic techniques. 14C-centchroman showed a low affinity binding and did not compete for 3H-cortisol or 3H-DHT binding sites in plasma. 14C-centchroman binding protein was heat stable in nature and showed the electrophoretic pattern similar to that of albumin (Rf 0.70). Thus centchroman binds to albumin in monkey plasma which can be suggested as carrier protein for this contraceptive agent.
Agnihotri
A
A
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Srivastava
A K
AK
Kamboj
V P
VP
D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002486:Centchroman / Q000097:blood*; D013196:Dihydrotestosterone / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D007700:Kinetics; D008253:Macaca mulatta; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism; D012710:Serum Albumin, Bovine; D014316:Tritium
D001798:Blood Proteins; D002250:Carbon Radioisotopes; D012709:Serum Albumin; D013196:Dihydrotestosterone; D014316:Tritium; D012710:Serum Albumin, Bovine; D002486:Centchroman; D006854:Hydrocortisone