Pseudomonas (Burkholderia) cepacia in children with cystic fibrosis: epidemiological investigation by analysis or restriction fragment length polymorphism.
Since 1987, Pseudomonas cepacia has been isolated with an increasing frequency in the expectorants of children with cystic fibrosis followed at the Hôpital d'enfants Armand Trousseau (Paris, France). Colonization by P. cepacia may be responsible for serious secondary infections and rapid deterioration in respiratory function in these patients. Among the 130 children attending our centre, 14 (8 girls and 6 boys) aged 3 to 18, exhibited chronic colonization. 132 isolates, originating from sputum obtained between 1992 and 1994 were studied. Nine biochemical patterns and 6 antibiotic susceptibility patterns at least were defined, therefore exhibiting great polymorphism. Chromosome restriction patterns with Xba I after pulsed field gel electrophoresis enabled 4 pulsotypes to be identified: A, B, C and D. Thirteen patients harboured pulsotypes A, C and D, and 1 patient pulsotype B, the last being quite distinct from the first three. Pulsotypes A, C and D were almost similar, suggesting that closely related strains, probably the same strain, was harboured by 13 of the 14 patients. The origin could be contamination from a single source, or stem from patient-to-patient crossed transmission.
Valcin
M
M
Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, Paris, France.
Moissenet
D
D
Sardet
A
A
Tournier
G
G
Garbarg-Chenon
A
A
Vu-Thien
H
H
D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016956:Burkholderia cepacia / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry*; D004359:Drug Therapy, Combination / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005602:France; D006776:Hospitals, Pediatric; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial