Production and characterization of monoclonal antibodies against L-carnitine: radioimmunologic assays for L-carnitine determination.
Monoclonal antibodies against L-carnitine have been produced and characterized. These antibodies have been found to specifically bind L-carnitine and, with different affinities, other carnitine-related compounds. No binding was observed with choline or acetylcholine. These antibodies have been used to measure L-carnitine in biological samples and serum. Data obtained demonstrate that, in biological samples, by using radiolabelled carnitine, it is possible quickly to detect small amounts of carnitine. The high specificity of the test is clearly demonstrated.
Marini
S
S
Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata," Italy. marinis@utovrm.it
Fasciglione
G F
GF
Giardina
B
B
D000109:Acetylcholine / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002331:Carnitine / Q000032:analysis / Q000276:immunology*; D002794:Choline / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D002794:Choline; D000109:Acetylcholine; D002331:Carnitine