Sulfatide prolongs blood-coagulation time and bleeding time by forming a complex with fibrinogen.
Sulfatides (galactosylceramide I3-sulfate), which are found in serum lipoproteins of various mammals, effectively increased prothrombin time (anticoagulant effect) and also effectively prolonged bleeding time (anti-platelet effect). When equal volumes of a homogeneous micellar solution of sulfatide and fibrinogen in phosphate-buffered saline were mixed, an insoluble complex precipitated. Analysis of the precipitated complex showed that the molar ratio of sulfatide to fibrinogen was about 400:1. These results indicate that the sulfatide micelle binds tightly to fibrinogen and thereby interferes with both fibrin gel formation (anticoagulant activity) and platelet function.
Hara
A
A
Research Center on Aging and Adaptation Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Uemura
K
K
Taketomi
T
T
D000818:Animals; D000925:Anticoagulants / Q000097:blood / Q000494:pharmacology*; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism*; D005782:Gels; D006028:Glycosphingolipids / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008297:Male; D008322:Mammals; D051379:Mice; D008823:Micelles; D011485:Protein Binding; D011517:Prothrombin Time; D011817:Rabbits; D013116:Spinal Cord; D013433:Sulfoglycosphingolipids / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine
D000925:Anticoagulants; D005782:Gels; D006028:Glycosphingolipids; D008823:Micelles; D013433:Sulfoglycosphingolipids; D005337:Fibrin; D005340:Fibrinogen