An enzyme-linked immunosorbent assay for the quantitative determination of anti-D in plasma samples and immunoglobulin preparations.
Here we present an enzyme-linked immunosorbent assay (ELISA) on microtiter plates for the quantitative determination of anti-D. This method is based on the solubilization of red blood cells sensitized with anti-D and the subsequent measurement of immunoglobulin G by ELISA. The measuring range of the assay is 40-5,000 IU/ml and the lowest quantifiable concentration in plasma is 0.5 IU/ml. The interassay relative standard deviation for concentrations above 130 IU/ml ranges from 3.2 to 8.1% and below 50 IU/ml from 10.5 to 19.7%. Comparison of ELISA with automated hemagglutination shows that the results of the two assays correlate well: r = 0.992, n = 26. The assay was validated for donor plasma samples and anti-D immunoglobulin preparations and it can also be used in assessing the severity of Rh (D) hemolytic disease during pregnancy.
Hirvonen
M
M
Finnish Red Cross Blood Transfusion Service, Helsinki, Finland.
Tervonen
S
S
Pirkola
A
A
Sievers
G
G
D000918:Antibody Specificity; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006386:Hemagglutination Tests; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification*; D007518:Isoantibodies / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin
D007136:Immunoglobulins; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin