Intracellularly applied anti-P70 antibody blocks the induction of abnormal membrane properties by pentylenetetrazole in identified Euhadra neurons.
Using the voltage-clamp technique combined with pressure injection, we have studied the action of pentylenetetrazole (PTZ) on identified Euhadra neurons by examining how the PTZ-induced changes in membrane properties are affected by an antibody against P70, a protein found in the experimentally-induced epileptogenic cortex of rats. Intracellular injection of anti-P70 antibody blocked the induction by PTZ; bursting activity with both of development of negative slope resistance region in the steady state 1-V curve and a reduction in the delayed outward potassium current. These results suggest a novel mechanism of action for PTZ, involving intracellular protein(s) which react with anti-P70 antibody.
Onozuka
M
M
Department of Anatomy, Gifu University School of Medicine [2nd Division], Japan.
Watanabe
K
K
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D003292:Convulsants / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004594:Electrophysiology; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010433:Pentylenetetrazole / Q000037:antagonists & inhibitors* / Q000633:toxicity; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D012908:Snails / Q000502:physiology*
D000906:Antibodies; D003292:Convulsants; D018840:HSP70 Heat-Shock Proteins; D015221:Potassium Channels; D010433:Pentylenetetrazole