Human interleukin-2 (IL-2) expressed by transfected mammalian cells.
cDNA for human interleukin-2 (IL-2) was cloned into the pRc/RSV vector for expression in animal cells. Baby hamster kidney (BHK-21) cells and Chinese hamster ovary (CHO) cells were transfected several times using calcium phosphate and electroporation methods with the construct pRc/RSV SIGIL2. Different transfection efficiencies were obtained. The biological test on CTLL-2 (mouse cytotoxic lymphocytes) showed that the kinetics of cell proliferation were different from those of rIL-2 (recombinant IL-2) expressed in bacteria and in BHK cells. When high concentrations of rIL-2 were applied, an inhibitory effect on CTLL-2 was observed when bacterial product was used, whilst rBHK interleukin caused no inhibition. Recombinant BHK IL2 induced a slower response of CTLL-2 cells at the beginning of the cultivation, however, prolonged activity was detected at the later stage of the experiment.
Knezević
M
M
BIA Ltd., Ljubljana, Slovenia.
Vorauer-Uhl
K
K
Hohenwarter
O
O
Steindl
F
F
Grabherr
R
R
Raspor
P
P
D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection
D007376:Interleukin-2; D011994:Recombinant Proteins; D015247:Deoxyribonuclease HindIII