GM1 ganglioside modulates prostaglandin E1 stimulated adenylyl cyclase in neuro-2A cells.
This study demonstrates modulation by GM1 ganglioside of prostaglandin E1 (PGE1)-induced cAMP formation in Neuro-2a neuroblastoma cells. Pretreatment of the cells with neuraminidase, an enzyme that increases cell surface GM1, resulted in significant elevation of PGE1-induced cAMP formation, as did preincubation of the cells with nmolar concentrations of GM1. Pretreatment with brain ganglioside mixture lacking GM1 had no effect. Cholera toxin B subunit, a specific GM1-binding ligand, inhibited adenylyl cyclase. When the concentration of exogenous GM1 in which the cells were preincubated was increased from nmolar to mu molar levels there was a dose-responsive fall off in cAMP elevation, attributed to progressive inhibition of adenylyl cyclase by increasing GM1. These results are interpreted as indicating modulation of this PGE1 receptor in Neuro-2a cells by plasma membrane-localized GM1 in a structure-specific manner.
Wu
G
G
New Jersey Medical School, UMDNJ, Department of Neurosciences, Newark 07103, USA.
Lu
Z H
ZH
Ledeen
R W
RW
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001921:Brain; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D005677:G(M1) Ganglioside; D002772:Cholera Toxin; D000242:Cyclic AMP; D009439:Neuraminidase; D000262:Adenylyl Cyclases; D000527:Alprostadil