title: Extended incubation of culture plates improves recovery of Bordetella spp.
abstract: Extended incubation of culture plates was studied to see if the recovery of Bordetella spp. from nasopharyngeal swabs could be improved. Forty-eight Bordetella isolates were recovered from 103 children (overall positive-culture rate, 46.6%) who met the clinical case definition of pertussis. Seven of 44 (16%) B. pertussis isolates and 2 of 4 (50%) B. parapertussis isolates were recovered only after extended incubation of nasopharyngeal cultures up to 12 days.
authors: Department of Clinical Laboratories (Microbiology), Alberta Children's Hospital, University of Calgary, Canada.
authors: G
authors: G
authors: Katzko
authors: M
authors: M
authors: Hofmeister
authors: D
authors: D
authors: Church
mesh_terms: D001431:Bacteriological Techniques; D001884:Bordetella / Q000254:growth & development / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000175:diagnosis; D001886:Bordetella pertussis / Q000254:growth & development / Q000302:isolation & purification; D002648:Child; D005069:Evaluation Studies as Topic; D006801:Humans; D013997:Time Factors; D014917:Whooping Cough / Q000175:diagnosis