Frequency of the three STR loci (TPOX, CSF1PO, THo1), in a Japanese population determined using a Gene Print STR multiplex kit.
Allele and genotype frequencies for three tetrametric short tandem repeat loci (TPOX, CSF1PO and TH01) were determined in a Japanese population sample (n = 105) by means of the Gene Print STR Multiplex system PCR kit, electrophoresis of the PCR products in denaturing gels and detection of allelic fragments by silver staining. Six alleles each were found for the TPOX, CSF1PO, and TH01 loci and at the TPOX locus, we identified an additional mutation allele, which consisted of 7 AATG repeats. The TPOX, CSF1PO and TH01 loci were apparently 64, 73, and 74% heterozygous, respectively. All loci met the Hardy-Weinberg criteria using categorized alleles.
Yamaguchi
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H
Postgraduate Course of Engineering, Toyama University, Japan.
Takizawa
H
H
Shimasaki
C
C
D000483:Alleles; D004586:Electrophoresis; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012494:Sampling Studies; D016622:Silver Staining