Fertilization and embryo cleavage after intracytoplasmic spermatid injection in an obstructive azoospermic patient with defective spermiogenesis.
OBJECTIVE
To achieve fertilization and cleavage by spermatids without tails from testicular biopsy.


METHODS
Clinical trial.


METHODS
Reproductive unit of a university teaching hospital.


METHODS
A patient of obstructive azoospermia with defective spermiogenesis.


METHODS
Testicular biopsy after scrotal exploration and spermatid injection into the cytoplasm of oocyte.


METHODS
Fertilization and cleavage.


RESULTS
Four of 13 spermatid injections achieved normal fertilization and 2 of them cleaved.


CONCLUSIONS
Intracytoplasmic spermatid injection may be a possible treatment for patients of defective spermiogenesis who have no viable spermatozoa available.
Chen
S U
SU
College of Medicine, National Taiwan University, Taipei.
Ho
H N
HN
Chen
H F
HF
Tsai
T C
TC
Lee
T Y
TY
Yang
Y S
YS
D000328:Adult; D002970:Cleavage Stage, Ovum; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007267:Injections; D008297:Male; D008846:Micromanipulation; D009845:Oligospermia / Q000503:physiopathology / Q000628:therapy*; D009865:Oocytes; D013087:Spermatids; D013091:Spermatogenesis