Ethanol stimulates diazepam binding inhibitor (DBI) mRNA expression in primary cultured neurons.
Changes in expression of diazepam binding inhibitor (DBI) mRNA in cerebral cortical neurons following long-term ethanol (EtOH) exposure were examined. A significant increase in DBI mRNA expression was observed by the exposure of neurons to 50 mM EtOH for up to 5 days and to EtOH (1-100 mM) for 3 days. These EtOH-induced increases in DBI mRNA expression were further elevated after the additional cultivation of neurons under EtOH-free condition. beta-Actin mRNA expression was not altered by similar EtOH treatments. These results indicate that EtOH possesses the activity to increase the expression of DBI mRNA in cerebral cortical neurons.
Katsura
M
M
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Ohkuma
S
S
Jun
X
X
Tsujimura
A
A
Kuriyama
K
K
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D026261:Diazepam Binding Inhibitor; D000431:Ethanol / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011963:Receptors, GABA-A / Q000187:drug effects; D013268:Stimulation, Chemical
D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D000431:Ethanol