The effect of tetraethylammonium on intracellular calcium concentration in Alzheimer's disease fibroblasts with APP, S182 and E5-1 missense mutations.
It has been proposed that the lack of intracellular calcium concentration ([Ca2+]i) increase induced by the potassium channel blocker tetraethylammonium (TEA) in skin fibroblast cell lines identifies patients with both sporadic and familial Alzheimer's disease (AD). In order to verify this hypothesis, the effect of TEA on [Ca2+]i was studied in single fura-2-loaded skin fibroblast cell lines available in the Tissue Bank of the Italian Research Council. Four out of eight familial AD patients (one patient with S182 mutation, one patient with E5-1 mutation and two patients with 717 Val-->Ile APP mutation) and two out of five sporadic AD patients showed a positive response to TEA, whereas five out of 11 control lines were unresponsive. Our data suggest that the absence of the TEA-induced increase in [Ca2+]i in skin fibroblast cell lines does not identify all AD patients.
Failli
P
P
Department of Pharmacology, University of Florence, Italy.
Tesco
G
G
Ruocco
C
C
Ginestroni
A
A
Amaducci
L
L
Giotti
A
A
Sorbi
S
S
D000368:Aged; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*
D013757:Tetraethylammonium Compounds; D002118:Calcium