title: Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells.
abstract: Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.
authors: Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany.
authors: B K
authors: BK
authors: Müller
authors: P
authors: P
authors: Kabos
authors: B
authors: B
authors: Belhage
authors: T
authors: T
authors: Neumann
authors: S B
authors: SB
authors: Kater
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology; D016257:Fura-2 / Q000494:pharmacology; D006706:Homeostasis; D007476:Ionophores / Q000494:pharmacology; D010320:Parvalbumins / Q000235:genetics / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D018243:Teratocarcinoma; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D007476:Ionophores; D010320:Parvalbumins; D000001:Calcimycin; D011189:Potassium Chloride; D002118:Calcium; D016257:Fura-2