[Study on asialoglycoprotein receptor mediated endocytosis of antiviral conjugate].
OBJECTIVE
To observe the internalization process of asialoglycoprotein receptor mediated antiviral conjugate lactosaminated human albumin arabinoside adenine monophosphate (L-HSA-Ara-AMP) in human hepatoma cell line HepG-2, and provide the specificity and practicability of receptor targeting treatment of viral hepatitis.


METHODS
Colloidal gold labelling technique combined with electronmicroscopic observation was employed. The study was carried out in three groups: experiment group (the internalization of antiviral conjugate in HepG-2 cell line), positive control group (lactosaminated human serum albumin), and negative control group and (human serum albumin).


RESULTS
L-HSA-Ara-AMP can be specially recognized and uptaken by the cells and enter the cells via coated pit. After internalization, the antiviral conjugate entered the lysosome ultimately.


CONCLUSIONS
The therapy of asialoglycoprotein receptor targeting treatment of viral hepatitis has a high specificity. The therapy is worth further study.
Ju
L
L
PLA 302nd Hospital, Beijing.
Huang-fu
Y
Y
Zhang
L
L
D000998:Antiviral Agents / Q000494:pharmacology*; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000473:pathology; D008247:Lysosomes / Q000378:metabolism*; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure; D001084:Vidarabine Phosphate / Q000494:pharmacology*
D000998:Antiviral Agents; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D000922:Immunotoxins; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D001084:Vidarabine Phosphate