Modeling the structure of the combining site of an antisweet taste ligand monoclonal antibody NC10.14.
We report the predicted combining site structure of the monoclonal antibody fragment, NC10.14, which is specific for the superpotent sweetener, N-(p-cyanophenyl-N'-(diphenylmethyl) guanidine acetic acid, using computer-aided molecular modeling and experimental methods, such as fluorescence spectroscopy and circular dichroism. This is the first computer-aided modeling study on a lambda-chain antibody fragment. We have also identified the amino acids that are involved in ligand binding. Aromatic residues, L:91(W), L:96(W), and H:100G(Y) are predicted to make van der Waals contacts with the p-cyanophenyl moiety of the ligand. Residue H:56(K) is predicted to provide a counterion for the acetic acid moiety, and H:50(E) provides the negatively charged potential for interaction with the positive guanidinium group. We also make a comparison of the binding site architecture of NC10.14 with that of a related monoclonal antibody fragment NC6.8.
Viswanathan
M
M
Department of Physiology and Biophysics, Beckman Institute, University of Illinois, Urbana 61801, USA.
Subramaniam
S
S
Pledger
D W
DW
Tetin
S Y
SY
Linthicum
D S
DS
D000085:Acetates / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics; D001666:Binding Sites, Antibody / Q000235:genetics; D003198:Computer Simulation; D006146:Guanidines / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013549:Sweetening Agents / Q000737:chemistry*
D000085:Acetates; D000911:Antibodies, Monoclonal; D006146:Guanidines; D008024:Ligands; D013549:Sweetening Agents; C083643:NC 174