[Prolongation of allogenic rat renal graft survival after perfusion the organ with anti-MHC class II antibody].
One of the main factor inducing rejection of the allogenic graft are the donor MHC-class II antigens. The cytotoxic damage or the elimination of these cells from the organ may influence his function after grafting. In this study, the influence of the continuous perfusion of the rat kidney with monoclonal antibody against rat MHC-class II antigens (MoAb 25D5) on graft survivals was investigated. For the perfusion, the HTK solution containing MoAb 25D5, rat complement (C) and Pentoxifilline (Ptx) was used. After one-hour perfusion at 21 degrees C kidney were transplanted to the syngenic (LEW-LEW) or allogenic (Da-LEW) recipients, kidney perfused with the solution containing MoAb 25D5 and C survived statistically longer (14.0 +/- 2.4 days) than in the control group without perfusion (7.7 +/- 0.5 days) or after perfusion with the solution containing only Ptx (7.8 +/- 0.8 days). It seems likely that the continuous perfusion of the rat kidney with HTK protective solution containing MoAb against MHC-class II antigens and C can reduce the immunogenicity of the graft.
Oko
A
A
Z Pracowni Immunopatologii Chorób, Nerek i Zgodności Tkanek, Akademii Medycznej w Poznaniu.
Krzymański
M
M
Idasiak-Piechocka
I
I
Pawliczak
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D006085:Graft Survival / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D010431:Pentoxifylline / Q000008:administration & dosage; D010477:Perfusion; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D010431:Pentoxifylline
Przedłuzenie przezycia allogenicznego przeszczepu nerki szczura pod wpływem perfuzji narzadu przeciwciałem monoklonalnym skierowanym przeciwko antygenom zgodności tkankowej klasy II.