Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases.
It is well established that sequence templates (e.g., PROSITE) and databases are powerful tools for identifying biological function and tertiary structure for an unknown protein sequence. Here we describe a method for automatically deriving 3D templates from the protein structures deposited in the Brookhaven Protein Data Bank. As an example, we describe a template derived for the Ser-His-Asp catalytic triad found in the serine proteases and triacylglycerol lipases. We find that the resultant template provides a highly selective tool for automatically differentiating between catalytic and noncatalytic Ser-His-Asp associations. When applied to nonproteolytic proteins, the template picks out two "non-esterase" catalytic triads that may be of biological relevance. This suggests that the development of databases of 3D templates, such as those that currently exist for protein sequence templates, will help identify the functions of new protein structures as they are determined and pinpoint their functionally important regions.
Wallace
A C
AC
Department of Biochemistry and Molecular Biology, University College, London, England.
Laskowski
R A
RA
Thornton
J M
JM
D000591:Amino Acid Isomerases / Q000737:chemistry; D001224:Aspartic Acid / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry; D016208:Databases, Factual; D006639:Histidine / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D019696:Peptidylprolyl Isomerase; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D017421:Sequence Analysis / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry; D012697:Serine Endopeptidases / Q000737:chemistry*; D013698:Templates, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011506:Proteins; D001224:Aspartic Acid; D012694:Serine; D006639:Histidine; D008049:Lipase; D012697:Serine Endopeptidases; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase