D2-family receptor distribution in human postmortem tissue: an autoradiographic study.
The distribution throughout the normal human brain of the dopamine D2-family of receptors were investigated autoradiographically. Three ligands were used, [3H]-YM-09151-2 to define the D2, D3, D4 receptors; [3H]raclopride the D2 D3 receptors; and [3H](+)-7-OH-DPAT, in the presence of GTP, demonstrates D3 distribution. [3H]-YM-09151-2 and [3H]raclopride binding were highest in caudate (121 vs 130 fmol mg(-1)), putamen (96 vs 136 fmol mg(-1)), and nucleus accumbens (113 vs 120 fmol mg(-1)). [3H]-YM-09151-2 also displayed significant binding in several cortical areas (56-39 fmol mg(-1)) and hippocampus (27 fmol mg-1). [3H](+)-7-OH-DPAT was highest in the nucleus accumbens. Based upon the ligands properties it is inferred that D2 distribution is highest in putamen, caudate and nucleus accumbens; D3 in the nucleus accumbens; D4 receptor in cortical areas and hippocampus.
Lahti
R A
RA
Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore, 21228, USA.
Roberts
R C
RC
Tamminga
C A
CA
D000328:Adult; D000368:Aged; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003024:Clozapine / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D011180:Postmortem Changes; D017448:Receptors, Dopamine D2 / Q000187:drug effects / Q000378:metabolism*; D013788:Thalamus / Q000378:metabolism; D014018:Tissue Distribution
D017448:Receptors, Dopamine D2; D003024:Clozapine