The effect of rebamipide on gastric xanthine oxidase activity and type conversion in ethanol-treated rats.
Rebamipide, a novel antipeptic ulcer drug, 2-(4-chlorobenzoylamino)-3-[2(1H)-quinolinone-4-yl]-propionic acid, was studied for its inhibitory effect on gastric xanthine oxidase activity and type conversion of the enzyme that has a profound role in free radical generation. Intraperitoneal administration of rebamipide at 60 mg/kg body weight reduced gastric mucosal hemorrhagic lesions and lipid peroxidation, which was proportional to the inhibitory effect of rebamipide on alcohol-induced xanthine oxidase-type conversion and enzyme activity. It was also observed that the activity of xanthine oxidase was significantly inhibited by administration of rebamipide at 60 mg/kg body weight, leading to a significant reduction of lipid peroxide content in alcohol-treated rats. The results suggest that alcohol-induced gastric mucosal lesions might be, in part, due to the increased activity of xanthine oxidase and type conversion rate of the enzyme and the protective effect of rebamipide on gastric mucosal lesions would result from its ability to protect against oxidative stress on gastric mucosal lesions of alcohol-treated rats.
Huh
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Department of Pharmacology, College of Pharmacy, Yeungnam University, Gyongsan, Korea.
Shin
U S
US
Lee
S H
SH
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D000431:Ethanol; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010438:Peptic Ulcer Hemorrhage / Q000139:chemically induced / Q000188:drug therapy; D015363:Quinolones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
D000897:Anti-Ulcer Agents; D004791:Enzyme Inhibitors; D005609:Free Radicals; D015363:Quinolones; D017382:Reactive Oxygen Species; D000431:Ethanol; D014969:Xanthine Oxidase; C052785:rebamipide; D000409:Alanine