Effect of emodin on c-myc proto-oncongen expression in cultured rat mesangial cells.
OBJECTIVE
To explore the possible molecular mechanism of emodin on the inhibition of glomerular mesangial cells (MC).


METHODS
In rat MC culture, c-myc mRNA level was detected by dot-blotting as expressed by the dilutions of the total RNA on the visible dots.


RESULTS
Low level c-myc mRNA was found in serum-free cultured quiescent MC. Addition of lipopolysaccharide (LPS 10 mg.L-1) induced a higher level expression of c-myc mRNA within 30 min, maximal expression at 2.5 h and persisted for 6 h. This over-expressing of c-myc mRNA was markedly suppressed by emodin (25 mg.L-1), the inhibition of emodin on c-myc mRNA expression was still seen at 6 h, and the greatest suppressive effect was at 2.5 h.


CONCLUSIONS
Emodin participating in its down-regulatory effect of c-myc mRNA over-expression contributes to its inhibitory action on MC.
Liu
Z H
ZH
Institute of Nephrology, Jinling Hospital, Nanjing, China.
Li
L S
LS
Hu
W X
WX
Zhou
H
H
D000818:Animals; D002478:Cells, Cultured; D004642:Emodin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D016259:Genes, myc; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012076:Renal Agents / Q000494:pharmacology*
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012076:Renal Agents; D004642:Emodin