Preliminary crystallographic analysis of the oxidized form of a two mono-nuclear iron centres protein from Desulfovibrio desulfuricans ATCC 27774.
Crystals of the fully oxidized form of desulfoferrodoxin were obtained by vapor diffusion from a solution containing 20% PEG 4000, 0.1 M HEPES buffer, pH 7.5, and 0.2 M CaCl2. Trigonal and/or rectangular prisms could be obtained, depending on the temperature used for the crystal growth. Trigonal prisms belong to the rhombohedral space group R32, with a = 112.5 A and c = 63.2 A; rectangular prisms belong to the monoclinic space group C2, with a = 77.7 A, b = 80.9 A, c = 53.9 A, and beta = 98.1 degrees. The crystallographic asymmetric unit of the rhombohedral crystal form contains one molecule. There are two molecules in the asymmetric unit of the monoclinic form, in agreement with the self-rotation function.
Coelho
A V
AV
Instituto de Tecnologia Química e Biológica, Oeiras, Portugal.
Matias
P M
PM
Carrondo
M A
MA
Tavares
P
P
Moura
J J
JJ
Moura
I
I
Fülop
V
V
Hajdu
J
J
Le Gall
J
J
D003460:Crystallization; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000737:chemistry / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry*; D010084:Oxidation-Reduction
D005288:Ferredoxins; C066602:desulfoferrodoxin