title: Inhibition of platelet aggregation by endocardial endothelial cells.
abstract: We assessed the anti-platelet properties of endocardial endothelial cells (EECs) by measuring platelet aggregation after brief interaction with EECs isolated from the right ventricles of porcine hearts. Platelet aggregation in response to thrombin was significantly inhibited by brief incubation of platelet suspensions over EEC monolayers. Pretreatment of EECs with indomethacin restored platelet reaction but that with L-NAME and hemoglobin (Hb) did not. The PGI2 content of platelet suspensions after interaction with cultured EECs was significantly correlated with the inhibition of platelet aggregation. These results suggest that EECs inhibit platelet aggregation by releasing PGI2.
authors: First Department of Surgery, Shimane Medical University, Japan.
authors: S
authors: S
authors: Nosaka
authors: K
authors: K
authors: Nakayama
authors: M
authors: M
authors: Hashimoto
authors: T
authors: T
authors: Sasaki
authors: K
authors: K
authors: Gu
authors: Y
authors: Y
authors: Saitoh
authors: S
authors: S
authors: Kin
authors: M
authors: M
authors: Yamauchi
authors: S
authors: S
authors: Masumura
authors: K
authors: K
authors: Tamura
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004699:Endocardium / Q000166:cytology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D011464:Epoprostenol / Q000378:metabolism; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D013552:Swine; D013917:Thrombin / Q000494:pharmacology
chemical_list: D010975:Platelet Aggregation Inhibitors; D001120:Arginine; D011464:Epoprostenol; D013917:Thrombin; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin