Phenotyping of esterase D and acid phosphatase by rapid isoelectric focusing on PhastSystem.
A rapid isoelectric focusing method on PhastSystem was applied to investigate the distribution of esterase D (ESD) and acid phosphatase (ACP) phenotypes in 152 and 112 unrelated healthy donors, respectively, from the Han population in Beijing. Laboratory-made gels with a pH range of 5.0-7.0 and 5.0-8.0 were used for analysis of ESD and ACP, respectively, with 1 microliter sample loading. The running time was 30-40 min. Gene frequencies for ESD were 0.6809 for ESD*1 and 0.3191 for ESD*2, (sigma chi 2 = 0.8586 and P > or = 0.50); gene frequencies of ACP were 0.2009 for ACP*A, 0.7991 for ACP*B, (sigma chi 2 = 1.7892 and P > 0.20), with good agreement between the observed and the expected values.
Guo
Y
Y
Institute of Biophysics, Academia Sinica, Beijing, China.
Huang
L
L
Yao
X
X
Yu
Z
Z
D000135:Acid Phosphatase / Q000032:analysis* / Q000235:genetics; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D010641:Phenotype; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors
D011933:Reagent Kits, Diagnostic; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D000135:Acid Phosphatase