Collateral sprouting of uninjured primary afferent A-fibers into the superficial dorsal horn of the adult rat spinal cord after topical capsaicin treatment to the sciatic nerve.
That terminals of uninjured primary sensory neurons terminating in the dorsal horn of the spinal cord can collaterally sprout was first suggested by Liu and Chambers (1958), but this has since been disputed. Recently, horseradish peroxidase conjugated to the B subunit of cholera toxin (B-HRP) and intracellular HRP injections have shown that sciatic nerve section or crush produces a long-lasting rearrangement in the organization of primary afferent central terminals, with A-fibers sprouting into lamina II, a region that normally receives only C-fiber input (Woolf et al., 1992). The mechanism of this A-fiber sprouting has been thought to involve injury-induced C-fiber transganglionic degeneration combined with myelinated A-fibers being conditioned into a regenerative growth state. In this study, we ask whether C-fiber degeneration and A-fiber conditioning are both necessary for the sprouting of A-fibers into lamina II. Local application of the C-fiber-specific neurotoxin capsaicin to the sciatic nerve has previously been shown to result in C-fiber damage and degenerative atrophy in lamina II. We have used B-HRP to transganglionically label A-fiber central terminals and have shown that 2 weeks after topical capsaicin treatment to the sciatic nerve, the pattern of B-HRP staining in the dorsal horn is indistinguishable from that seen after axotomy, with lamina II displaying novel staining in the identical region containing capsaicin-treated C-fiber central terminals. These results suggest that after C-fiber injury, uninjured A-fiber central terminals can collaterally sprout into lamina II of the dorsal horn. This phenomenon may help to explain the pain associated with C-fiber neuropathy.
Mannion
R J
RJ
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Doubell
T P
TP
Coggeshall
R E
RE
Woolf
C J
CJ
D000287:Administration, Topical; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D002772:Cholera Toxin; D003714:Denervation; D005727:Ganglia, Spinal / Q000201:enzymology; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D009416:Nerve Regeneration; D009473:Neuronal Plasticity; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects*; D013116:Spinal Cord / Q000502:physiology*; D013194:Staining and Labeling
C052635:cholera toxin, B subunit-horseradish peroxidase; D002772:Cholera Toxin; D006735:Horseradish Peroxidase; C022734:thiamine monophosphatase; D010744:Phosphoric Monoester Hydrolases; D002211:Capsaicin