title: Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures.
abstract: Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly-L-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.
authors: Laboratory of Environmental Neuroscience, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
authors: D M
authors: DM
authors: Bronstein
authors: I
authors: I
authors: Perez-Otano
authors: V
authors: V
authors: Sun
authors: S B
authors: SB
authors: Mullis Sawin
authors: J
authors: J
authors: Chan
authors: G C
authors: GC
authors: Wu
authors: P M
authors: PM
authors: Hudson
authors: L Y
authors: LY
authors: Kong
authors: J S
authors: JS
authors: Hong
authors: M K
authors: MK
authors: McMillian
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000633:toxicity*; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D018696:Neuroprotective Agents / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D016627:Oxidopamine / Q000633:toxicity*; D011107:Polylysine; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009498:Neurotoxins; D011107:Polylysine; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase