Detection of endothelial cell-derived nitric oxide: current trends and future directions.
The vascular endothelium is a significant site of NO release that inhibits cellular adhesion and maintains a non-thrombogenic surface. Use of newly described technology suggests for the first time that the maximal release of NO induced by cNOS and iNOS activation may be quite similar, implying that it is the duration of NO release and not the concentration of NO produced from stimulated endothelial cells that accounts for the different biological activities of the enzymes. The respective roles of cNOS and iNOS must be carefully evaluated since both enzymes may have potent biological effects at local sites of production.
Magazine
H I
HI
Department of Biology, Queens College, Flushing, New York, USA.
D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D006224:Cricetinae; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D051381:Rats; D013045:Species Specificity
D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D009249:NADP; D019001:Nitric Oxide Synthase