Two novel genes rapidly and transiently activated in suspension-cultured rice cells by treatment with N-acetylchitoheptaose, a biotic elicitor for phytoalexin production.
By using subtracted probes, two cDNA clones of rice, EL2 and EL3, were isolated as genes responsive within 6 min to N-acetylchitoheptaose, a potent biotic elicitor for phytoalexin biosynthesis. Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes.
Minami
E
E
Department of Cell Biology, National Institute of Agrobiological Resources, Japan.
Kuchitsu
K
K
He
D Y
DY
Kouchi
H
H
Midoh
N
N
Ohtsuki
Y
Y
Shibuya
N
N
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
D018076:DNA, Complementary; D018744:DNA, Plant; D009844:Oligosaccharides; D010936:Plant Extracts; D012333:RNA, Messenger; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins