[Plasma kallikrein clearance by the liver in experimental cirrhosis].
OBJECTIVE
To study the hepatic clearance of a glycoprotein (rat plasma kallikrein) by the liver of rats with experimental decompensated cirrhosis.


METHODS
Cirrhosis was induced by intragastrically administration of carbon tetrachloride 520 mg/kg/week, during 16-19 weeks. After this period, each liver was isolated, exsanguinated and perfused at 37 degrees C with 10nM rat plasma kallikrein (RPK).


RESULTS
58% of the animals died during the treatment and the remaining developed prostration, ascites, jaundice and bleeding; at the end of the treatment period serum aminotransferases were not altered and serum albumin decreased. The liver histology showed cirrhosis. RPK clearance rate of the cirrhosis group (5.4 +/- 0.9 pmol/g liver/10 min) was significantly lower (p < 0.05) than that of the control group (13.5 +/- 2.7 pmol/g liver/10 min).


CONCLUSIONS
The development of cirrhosis is associated with a decreased hepatic clearance of a glycoprotein which endocytosis is mediated by a receptor.
de Toledo
C F
CF
Laboratório de Hepatologia Experimental, Universidade Federal de São Paulo.
Loureiro-Silva
M R
MR
Molina
H M
HM
Lanzoni
V P
VP
Kouyoumdjian
M
M
Borges
D R
DR
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000008:administration & dosage*; D007610:Kallikreins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008106:Liver Cirrhosis, Experimental / Q000503:physiopathology*; D008657:Metabolic Clearance Rate; D009929:Organ Size / Q000187:drug effects; D010477:Perfusion; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar
D002251:Carbon Tetrachloride; D007610:Kallikreins
Depuração hepática da calicreína plasmática na cirrose experimental.