Donor-specific cellular immune response against human cardiac valve allografts.
We studied the presence of donor-specific T lymphocytes in explanted human cardiac valve allografts in vivo. From five of seven explants we propagated lymphocyte cultures in an interleukin-2 conditioned medium. Phenotyping revealed the presence of T-cell receptors in more than 95% of the lymphocytes obtained in each culture. Donor-specific cytotoxicity was demonstrated in three patients with known HLA status of the donor. Cytotoxicity was directed against only HLA class I in one patient, and against class I and/or class II in the others. These results indicate that donor-specific cellular reactivity can be induced by transplantation of human cardiac valve allografts.
Hoekstra
F
F
Department of Internal Medicine I, University Hospital Rotterdam, The Netherlands.
Knoop
C
C
Vaessen
L
L
Wassenaar
C
C
Jutte
N
N
Bos
E
E
Bogers
A
A
Weimar
W
W
D000328:Adult; D001021:Aortic Valve / Q000276:immunology / Q000637:transplantation*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007223:Infant; D007376:Interleukin-2; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D008875:Middle Aged; D010641:Phenotype; D011664:Pulmonary Valve / Q000276:immunology / Q000637:transplantation*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D003470:Culture Media; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell