Small scale biosynthesis and purification of gram quantities of chorismic acid.
This paper details improvements in the biosynthetic method of purification of chorismic acid using Klebsiella pneumoniae 62-1. New growth and accumulation conditions yielded 4 L of accumulation medium containing approximately 0.5 g/L of chorismate from 2 L of bacterial growth. Improvements in the handling and extraction procedures produced yields of approximately 2 g of 75 to 90% chorismic acid. A new recrystallization procedure yielded chorismic acid 97% pure by weight (using epsilon 275 = 2630 M-1 cm-1), and 99.8% pure by enzymatic conversion. These results represent a three- to five-fold increase in yield over average published values.
Rieger
C E
CE
Department of Chemistry and Biochemistry Concordia University, Montreal, Quebec.
Turnbull
J L
JL
D002827:Chorismic Acid / Q000302:isolation & purification* / Q000378:metabolism*; D003460:Crystallization; D017077:Culture Media, Conditioned; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*
D017077:Culture Media, Conditioned; D002827:Chorismic Acid