title: Characteristics of major structural protein coding gene and leader-body sequence in subgenomic mRNA of porcine reproductive and respiratory syndrome virus isolated in Japan.
abstract: Nucleotide sequence, 1713nt in length, of porcine reproductive and respiratory syndrome virus (PRRSV) isolated in Japan was determined. The sequence encompassed 3 overlapping open reading frames (ORFs), ORF5 to ORF7. These ORFs encodes major structural proteins of PRRSV. The deduced amino acid sequence of each ORF showed higher than 87.5% identity with an American isolate, and lower (54.6 to 80.5%) identity with an European isolate. This result supported a previous report about antigenic characteristics of the EDRD-1 strain. Leader-body junction sequence in subgenomic mRNA of the EDRD-1 strain was determined by sequencing cDNA clones of subgenomic RNAs. A common sequence motif of 5 nucleotide, represented by UA(A/G)CC, was identified as the junction sequence.
authors: National Veterinary Assay Laboratory, Tokyo, Japan.
authors: A
authors: A
authors: Saito
authors: T
authors: T
authors: Kanno
authors: Y
authors: Y
authors: Murakami
authors: M
authors: M
authors: Muramatsu
authors: S
authors: S
authors: Yamaguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018018:Arterivirus / Q000235:genetics* / Q000302:isolation & purification; D018174:Arterivirus Infections / Q000662:veterinary* / Q000821:virology; D001483:Base Sequence; D017931:DNA Primers; D007564:Japan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D013553:Swine Diseases; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D015678:Viral Structural Proteins