title: Electrophoretic analysis of biliary proteins: application of high resolution two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradient in the first dimension.
abstract: Native bile samples, dialyzed against double distilled water, fractionated by Sepharose 2B column chromatography, concentrated by ultrafiltration and lyophilized, were solubilized in lysis buffer and studied by two-dimensional electrophoresis (2-DE) with immobilized pH gradients in the first dimension. We counted 70, and 7 spots in 2-DE patterns of bile from patients with cholesterol gallstones and pigment gallstones, respectively.
authors: Department of Medicine II, Klinikum Grosshadern, Ludwig-Maximilians-University Munich, Germany.
authors: C
authors: C
authors: He
authors: D
authors: D
authors: Jngst
mesh_terms: D001646:Bile / Q000737:chemistry*; D002769:Cholelithiasis / Q000378:metabolism / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining
chemical_list: D011506:Proteins