Three dynamin-encoding genes are differentially expressed in developing rat brain.
Dynamin proteins are members of a recently described family of GTPases involved in receptor-mediated processes. To date, three different dynamin-encoding genes have been identified in mammalian tissues. Dynamin I is expressed only in neurons, whereas dynamin II is ubiquitously expressed. A third isoform, dynamin III, was originally isolated from a rat testis cDNA library and shown to be testis-specific. However, here we report the cloning and characterization of dynamin III from brain and lung, demonstrating a more extended pattern of expression for this isoform. In addition, we have investigated the temporal pattern of expression of these three genes during brain development. We find that both dynamin I and dynamin III mRNA levels are up-regulated during embryogenesis, whereas dynamin II mRNA levels remain unchanged. From these results, we conclude that dynamin III is not a testis-specific isoform and, furthermore, that rat brain expresses three different dynamin-encoding genes that are differentially regulated during development. Therefore, this large isoform diversity of dynamin proteins in brain predicts a significant complexity in the understanding of dynamin-based processes in this tissue.
Cook
T
T
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA.
Mesa
K
K
Urrutia
R
R
D017398:Alternative Splicing / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000502:physiology; D034284:Dynamin I; D034286:Dynamin III; D034281:Dynamins; D004705:Endocytosis / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015723:Gene Library; D005820:Genetic Testing; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008168:Lung / Q000502:physiology; D008297:Male; D008870:Microtubules / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016716:PC12 Cells / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000502:physiology; D013997:Time Factors
D007527:Isoenzymes; D012333:RNA, Messenger; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034286:Dynamin III; D034281:Dynamins