Kinetic nephelometric determination of albumin produced by rat hepatocytes in culture.
A kinetic immunonephelometric method for the determination of albumin produced by rat hepatocytes in culture is described. This assay is simple, rapid and sensitive. The methodology allows detection of 0.7 mg/l albumin in 200 microliters of culture medium. Within-run precision CVs for three levels of concentrations were under 1.0% and between-day precision CVs were under 4.1%. The range of measurement obtained using appropriately diluted samples was 1.2 to 74 mg/l. The rabbit IgG fraction to rat albumin used in this method did not cross-react with albumin from cow, allowing the use of fetal calf serum in the medium. The method described can thus be used easily for the assessment of albumin synthesis in cellular studies using isolated hepatocytes.
Pernet
P
P
Department of Biochemistry A, St-Antoine Hospital, Paris, France.
Vaubourdolle
M
M
Lim
S K
SK
Cynober
L
L
Giboudeau
J
J
D000418:Albumins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D003429:Cross Reactions; D007074:Immunoglobulin G / Q000276:immunology; D007158:Immunologic Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D007074:Immunoglobulin G