Platelet-activating factor produces neuronal growth cone collapse.
It is generally believed that neuronal growth cone collapsing factors are proteins that interact with membrane-bound receptors. Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) - a phospholipid autocoid, also interacts with a membrane-bound neuronal receptor which is similar in nature to collapsing factor receptors. We report that PAF and the nonhydrolyzable PAF agonist, methyl carbamyl PAF (1-O-hexadecyl-2N-methylcarbamyl-sn-glycero-3-phosphocholine, mc-PAF), evoke a dose-dependent neuronal growth cone collapse. This collapse is specifically attenuated by the PAF receptor antagonist BN-52021. These data point to a PAF receptor-mediated growth cone collapse. Therefore, PAF must be added to the list of collapsing factors which potentially guide axons to their proper targets in the developing nervous system.
Clark
G D
GD
The Cain Foundation Laboratories, Baylor College of Medicine, Houston, TX 77030, USA.
McNeil
R S
RS
Bix
G J
GJ
Swann
J W
JW
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects*; D000432:Methanol / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D010972:Platelet Activating Factor; D000432:Methanol