The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes.
Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethylaminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.
Ledda
S
S
University of Sassari, Department of Physiology, Italy.
Loi
P
P
Bogliolo
L
L
Moor
R M
RM
Fulka
J
J
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects*; D012756:Sheep; D013045:Species Specificity
C001020:N(6),N(6)-dimethyladenine; D000225:Adenine