Involvement of free radicals in the cardioprotective effect of defibrotide.
Ischemia followed by reperfusion has deleterious effects on myocardial tissue and a wide range of drugs have been investigated to modulate these changes. Defibrotide (polydeoxyribonucleotides from bovine lung), a drug with antithrombotic and fibrinolytic activities, has also proven to be cardioprotective against myocardial ischemia/reperfusion damage. However, the mechanism of this protective effect has not been clarified yet. The aim of this study was to determine whether this effect is due to protection against free radical induced changes. The experimental model in rabbits includes coronary artery ligation for 60 min followed by a reperfusion period of 45 min. In this model, free radical damage was estimated by different parameters of lipid peroxidation such as diene conjugation, carbonyl content, and thiobarbituric acid reactive substances, together with protein oxidation determinations. The results demonstrate that defibrotide prevents free radical induced changes after myocardial ischemia/reperfusion.
Haklar
G
G
Department of Biochemistry, Faculty of Medicine, Marmara University, Haydarpaşa-Istanbul, Turkey.
Erşahin
C
C
Moini
H
H
Süngün
M
M
Doğan
N
N
Bilsel
S
S
Emerk
K
K
Yalçin
A S
AS
D000818:Animals; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D011089:Polydeoxyribonucleotides / Q000627:therapeutic use*; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005343:Fibrinolytic Agents; D005609:Free Radicals; D011089:Polydeoxyribonucleotides; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; C036901:defibrotide