[Grouping and typing of Ureaplasma urealyticum].
OBJECTIVE
To construct the molecular biological methods to group and type Ureaplasma urealyticum.


METHODS
Polymerase chain reaction was used to detect and group the 14 standard serotypes of Ureaplasma urealyticum. 272 urogenital tract clinical samples were detected and grouped. Random amplified polymorphic deoxyribose nucleic acid was used to study and compare Ureaplasma urealyticum serotypes 1, 3, 6, 14, 8, 10, 11.


RESULTS
Detected by the polymerase chain reaction, group 1 had 403 bp amplified fragments, and group 2 had 448 bp amplified fragments. 272 urogenital tract clinical samples were detected. 72 cases were positive for ureaplasma urealyticum, 69 were positive for group 1, and 3 were positive for group 2. Random amplified polymorphic deoxyribose nucleic acid was used and gene fingerprinting maps of ureaplasma urealyticum serotypes 1, 3, 6, 14, 8, 10, 11 were constructed.


CONCLUSIONS
Ureaplasma urealyticum grouping method was constructed by polymerase chain reaction. Among the urogenital tract clinical samples positive for Ureaplasma urealyticum, group 1 was more common to be positive. Random amplified polymorphic deoxyribose nucleic acid can be used as typing method of Ureaplasma urealyticum.
Kong
F
F
Department of Dermatology, First Hospital, Beijing Medical University.
Zhu
X
X
Zhou
J
J
D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D016990:Ureaplasma urealyticum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial