Human myeloid cell nuclear differentiation antigen binds specifically to nucleolin.
The human myeloid cell nuclear differentiation antigen (MNDA) is a nuclear protein expressed specifically in cells of the myelomonocytic lineage and regulated by interferon alpha in a cell-specific fashion. MNDA is also a member of a family of interferon-regulated genes of unknown function. In an effort to elucidate the function of MNDA, three techniques (affinity purification, coimmunoprecipitation, and protein blot assay) were used to characterize its specific protein binding activities. Microsequence analysis showed that MNDA bound the 100 kDa nucleolin protein. The identification of nucleolin was confirmed by immunoreaction with specific antibodies. MNDA contains motifs which could account for specific binding to nucleolin. Nucleolin binds other macromolecules and exhibits features consistent with roles in signal transduction, production of ribosomes, nuclear matrix structure, and regulation of transcription. The present results indicate that the function of MNDA is most likely related to interactions with other proteins. Through these associations, MNDA could contribute cell/lineage- and differentiation-specific limits to the function of ubiquitous proteins such as nucleolin. Further analysis of MNDA protein binding could be critical to elucidating the function of MNDA and could contribute to understanding the function of the products of other members of this interferon-inducible family of genes.
Xie
J
J
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Briggs
J A
JA
Olson
M O
MO
Sipos
K
K
Briggs
R C
RC
D000595:Amino Acid Sequence; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis; D000939:Epitopes; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D009697:Nucleolus Organizer Region; D010750:Phosphoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured; D000097796:Nucleolin
D015214:Antigens, Differentiation, Myelomonocytic; D002352:Carrier Proteins; D000939:Epitopes; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D000097796:Nucleolin; C075373:MNDA protein, human