title: Anorexia induced by the parasitic nematode, Nippostrongylus brasiliensis: effects on NPY and CRF gene expression in the rat hypothalamus.
abstract: Infections of the gastrointestinal nematode, Nippostrongylus brasiliensis, in the laboratory rat result in a characteristic biphasic anorexia which is followed by hyperphagia once the worm burden has been cleared. Despite the importance of parasite-induced anorexia, relatively little is known of the underlying mechanisms. We have investigated the involvement of the central appetite drive in this anorexia by studying the gene expression of two neuropeptides with opposing actions on energy balance, NPY and CRF. Gene expression was assessed by in situ hybridization at 2, 8 and 16 days post-infection (p.i.) in infected rats, in uninfected controls, and in a group with food intake restricted to match that taken voluntarily by the parasitize animals. The sampling intervals corresponded to each of the two phases of maximum anorexia and the period of compensatory hyperphagia. Surprisingly, we found that increases in NPY gene expression in the hypothalamic arcuate nucleus (ARC) accompany anorexia in rats infected with N. brasiliensis; there was a significant relationship between degree of anorexia and induction of NPY mRNA after 8 days of infection. Furthermore, ARC NPY mRNA levels in parasitized animals were similar to those in pair-fed individuals with food intake restricted to match the infected rats. The number of larvae used to establish the infection affected both the degree of anorexia and the level of NPY mRNA at 8 days p.i. in a dose-dependent manner. NPY gene expression remained elevated in infected rats during at least the initial stages of compensatory hyperphagia. This suggests that animals detect a state of energy deficit during the early stages of the infection, yet do not feed, but become hyperphagic coincident with worm loss. The failure of anorectic parasitized animals to feed in response to activation of the NPYergic system makes this a novel system in which to study the regulation of hypothalamic NPY by physiological challenge. There were no significant differences in CRF gene expression between the groups at any of the sampling intervals.
authors: Department of Zoology, University of Aberdeen, Scotland, UK.
authors: S R
authors: SR
authors: Horbury
authors: J G
authors: JG
authors: Mercer
authors: L H
authors: LH
authors: Chappell
mesh_terms: D000818:Animals; D000855:Anorexia / Q000469:parasitology* / Q000503:physiopathology; D001835:Body Weight; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D004435:Eating; D005247:Feeding Behavior / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D017403:In Situ Hybridization; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics*; D009559:Nippostrongylus / Q000469:parasitology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone