Absence of double-stranded replicative forms of HCV RNA in liver tissue from chronically infected patients.
The mechanism of hepatitis C virus (HCV) replication is unknown, although the classification of HCV in the Flaviviridae has led to the postulation that HCV may adopt a replication strategy similar to that of the flaviviruses. To determine if HCV double-stranded replicative forms, consistent with this strategy, were present in total liver RNA extracted from HCV-infected individuals, HCV-specific RNA was detected by reverse transcription followed by polymerase chain reaction (RT-PCR). Initially, a strand-specific RT-PCR resulting from chemical modification of the 3' end of the RNA was established using in vitro transcribed HCV RNA. This procedure allowed the specific detection of positive and negative HCV RNA strands in HCV-infected liver tissue. The species of HCV RNA was then examined in RNA extracted from liver tissue from naturally infected individuals; total liver RNA was either: (i) fractionated with 2M LiCl (designed to precipitate single-stranded and partially double-stranded RNA); or (ii) digested with RNase A in high salt conditions (designed to digest single-stranded RNA only). Amplification of positive sense HCV RNA from the LiCl-insoluble fraction, but not from the LiCl-soluble fraction nor in the RNase A-digested sample, was consistent with the interpretation that single-strand, but not double-stranded HCV RNA, was contained in the liver samples. Thus, it is unclear if a double-stranded RNA species is formed during the HCV replication cycle.
Blight
K
K
Department of Microbiology and Immunology, University of Adelaide, Australia.
Trowbridge
R
R
Gowans
E J
EJ
D004587:Electrophoresis, Agar Gel; D005416:Flavivirus / Q000145:classification; D016174:Hepacivirus / Q000737:chemistry*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008099:Liver / Q000821:virology*; D016133:Polymerase Chain Reaction; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012260:Ribonucleases