Maillard induced complexes of bovine serum albumin--a dilute solution study.
Association of bovine serum albumin (BSA) on heating in the presence and absence of 2% xylose has been studied using dynamic light scattering and sedimentation velocity. When 3% solutions of the protein alone are heated at 95 degrees C association products are formed with molar masses of approximately 2 x 10(6) g/mol, a value which is independent of the time of heating. These aggregates can be dissociated in solvents that disrupt non-covalent bonds. When the reducing sugar xylose is present there is a continuous change in the hydrodynamic properties with time. After 80 min a molar mass in excess of 7 x 10(6) g/mol is obtained. This increase in molar mass is attributed to additional non-disulphide linkages resulting from the Maillard reaction. Information about the gross conformation of the Maillard induced association products has been obtained from MHKS (Mark-Houwink-Kuhn-Sakarada) double logarithmic plots of D20,w and S20,w against molar mass. The values of the MHKS coefficients obtained are most consistent with a linear rod: i.e. the association is of an end-to-end type.
Easa
A M
AM
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, UK.
Armstrong
H J
HJ
Mitchell
J R
JR
Hill
S E
SE
Harding
S E
SE
Taylor
A J
AJ
D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008027:Light; D046911:Macromolecular Substances; D015416:Maillard Reaction; D008970:Molecular Weight; D011487:Protein Conformation; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D014994:Xylose
D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D012996:Solutions; D012710:Serum Albumin, Bovine; D014994:Xylose