A new microplate red blood cell monolayer technique for screening and identifying red blood cell antibodies.
A new method has been developed to immobilize red blood cells in wells of microplates using a cell fixation buffer. This method has been employed for detecting and identifying red blood cell antibodies with greater sensitivity than haemagglutination antiglobulin tests, without loss of specificity. This method decreases the amount of test erythrocytes and anti-human globulin reagents employed per test, consequently lowering the cost.
Llopis
F
F
Regional Blood Transfusion Centre, Valencia, Spain.
Carbonell-Uberos
F
F
Planelles
M D
MD
Montero
M
M
Plasencia
I
I
Carrillo
C
C
D000906:Antibodies / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans
D000906:Antibodies