Metal-ion-dependent oxidative DNA cleavage by transition metal complexes of a new water-soluble salen derivative.
A new water-soluble, salen [salen = bis(salicylidene) ethylenediamine]-based ligand, 3 was developed. Two of the metal complexes of this ligand, i.e., 3a, [Mn(III)] and 3b, [Ni(II)], in the presence of cooxidant magnesium monoperoxyphthalate (MMPP) cleaved plasmid DNA pTZ19R efficiently and rapidly at a concentration approximately 1 microM. In contrast, under comparable conditions, other metal complexes 3c, [Cu(II)] or 3d, [Cr(III)] could not induce any significant DNA nicking. The findings with Ni(II) complex suggest that the DNA cleavage processes can be modulated by the disposition of charges around the ligand.
Mandal
S S
SS
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.
Kumar
N V
NV
Varshney
U
U
Bhattacharya
S
S
D001345:Autoradiography; D001483:Base Sequence; D004249:DNA Damage; D005029:Ethylenediamines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012995:Solubility; D013696:Temperature; D014867:Water / Q000737:chemistry
D005029:Ethylenediamines; D008670:Metals; D014867:Water; C011452:disalicylaldehyde ethylenediamine