Genetic control of the resistance to phage C1 of Escherichia coli K-12.
Escherichia coli K-12 lytic phage C1 was earlier isolated in our laboratory. Its adsorption is controlled by at least three bacterial genes: dcrA, dcrB, and btuB. Our results provide evidence that the dcrA gene located at 60 min on the E. coli genetic map is identical to the sdaC gene. This gene product is an inner membrane protein recently identified as a putative specific serine transporter. The dcrB gene, located at 76.5 min, encodes a 20-kDa processed periplasmic protein, as determined by maxicell analysis, and corresponds to a recently determined open reading frame with a previously unknown function. The btuB gene product is known to be an outer membrane receptor protein responsible for adsorption of BF23 phage and vitamin B12 uptake. According to our data the DcrA and DcrB proteins are not involved in these processes. However, the DcrA protein probably participates in some cell division steps.
Likhacheva
N A
NA
State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Samsonov
V V
VV
Samsonov
V V
VV
Sineoky
S P
SP
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003090:Coliphages / Q000472:pathogenicity*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology / Q000821:virology; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D026901:Membrane Transport Proteins; D009154:Mutation; D018000:Receptors, Peptide / Q000235:genetics / Q000502:physiology; D014805:Vitamin B 12 / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C044592:BtuB protein, E coli; C101655:DcrB protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D018000:Receptors, Peptide; C088329:SdaC protein, E coli; C085429:dcrA protein, Desulfovibrio vulgaris; D014805:Vitamin B 12