Radical scavenger activity of bendazac, an anticataract non-steroidal anti-inflammatory agent.
Oxidative damage to lens components is associated with cataract formation and reactive oxygen species (ROS) overproduction at inflammation sites is thought to lead to the development of inflammatory disorders. Bendazac is a non-steroidal anti-inflammatory drug able to delay the cataractogenic process. Aim of the present study is to characterize, both chemically and biologically, the activity of this anticataract agent as a radical scavenger. Bendazac has been shown to be a strong reacting substrate in a chemical oxidizing system, which mimics a physiological pathway of hydroxy radical generation. In the Fenton-Cier reaction the drug rapidly forms a mixture of hydroxylated derivatives, among which 5-hydroxybendazac, bendazac's main metabolite, being a hydroxy radical scavenger itself. Moreover, by means of a rapid and sensitive flow cytometric method able to determine reactive oxygen intermediate production, bendazac and its 5-hydroxy derivative were shown to inhibit oxidative burst activation in polymorphonuclear neutrophil leukocytes (PMNLs).
Guglielmotti
A
A
Lab. of Immunopharmacology, Angelini Ricerche, Rome, Italy.
Capezzone De Joannon
A
A
Cazzolla
N
N
Marchetti
M
M
Soldo
L
L
Cavallo
G
G
Pinza
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002386:Cataract / Q000517:prevention & control; D005260:Female; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000737:chemistry*; D007191:Indazoles / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D016897:Respiratory Burst / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016166:Free Radical Scavengers; D007191:Indazoles; D002374:Catalase; C000123:bendazac