Salvage pathway of pyrimidine synthesis: divergence of substrate specificity in two related species of teleostean fish.
For nucleotide synthesis, cells use purine and pyrimidine nucleosides generated either through de novo synthesis or through utilization of salvage pathways. In the pyrimidine salvage pathway, thymidine is taken up by transport proteins and phosphorylated by the enzyme thymidine kinase to thymidine monophosphate. So far, all vertebrates analyzed are able to use radioactively labeled thymidine for the biosynthesis of nucleotides in brain tissue. However, when standard autoradiographic, immunohistochemical and biochemical procedures were applied for the detection of the incorporation of tritiated thymidine and the thymidine analogue 5-bromo-2'-deoxyuridine into DNA to two species of gymnotiform fish, a divergence in substrate specificity has been revealed. Although brain cells of the two species, Apteronotus leptorhynchus and Eigenmannia sp., can utilize 5-bromo-2'-deoxyuridine for pyrimidine synthesis, only Eigenmannia sp. is able to incorporate tritiated thymidine into DNA during the S phase of the cell cycle. We hypothesize that this inability to use thymidine for nucleotide synthesis is caused either by a defect in the transport system mediating the uptake of thymidine or by a deficiency in the thymidine kinase of A. leptorhynchus.
Zupanc
G K
GK
Abteilung Physikalische Biologie, Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany. zupanc@mpib-tuebingen.mpg.de
Horschke
I
I
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004555:Electric Fish / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D008297:Male; D008938:Mitosis; D011743:Pyrimidines / Q000378:metabolism*; D011859:Radiography; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D011743:Pyrimidines; D014316:Tritium; D004247:DNA; D001973:Bromodeoxyuridine; C030986:pyrimidine; D013936:Thymidine