Note: inhibition of the growth of Yersinia enterocolitica O:3 by the microflora of porcine caecum and ileum in an in vitro model.
The growth of Yersinia enterocolitica O:3 was tested in an in vitro model of the porcine intestine at the physiological temperature of 39 degrees C of growing pigs. The model supported a stable population of Y. enterocolitica at a level 10(8)-10(9) cells ml-1. Plasmid profile analysis and the Ca(2+)-dependent proportion of the population suggested that the great majority of the Y. enterocolitica population retained the 70 kb virulence plasmid, pYV, throughout the experimental period of 5 d. The growth of Y. enterocolitica was substantially inhibited by the ileal and the caecal flora compared to the growth of the bacterium alone. Yersinia enterocolitica was not isolated after 3 d of cultivation.
Asplund
K
K
National Veterinary and Food Research Institute, Helsinki, Finland.
Hakkinen
M
M
Björkroth
J
J
Nuotio
L
L
Nurmi
E
E
D000818:Animals; D001421:Bacteria, Anaerobic / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D002432:Cecum / Q000382:microbiology*; D015169:Colony Count, Microbial; D007082:Ileum / Q000382:microbiology*; D008970:Molecular Weight; D010957:Plasmids / Q000032:analysis / Q000737:chemistry; D013552:Swine; D013696:Temperature; D014774:Virulence; D015008:Yersinia enterocolitica / Q000254:growth & development* / Q000472:pathogenicity
D002118:Calcium