Dynamic aspects of colicin N translocation through the Escherichia coli outer membrane.
Colicin N is a bacteriocin that kills sensitive Escherichia coli cells. After binding to the cell surface-exposed receptor, a short period exists when a significant number of the cell-associated colicin N molecules are sensitive to external enzymes. Two colicin N populations are discriminated by proteases: the susceptible pool bound to OmpF porin on the cell surface and another population corresponding to protease-inaccessible colicin N. During translocation, colicin N reaches the periplasmic space and proteolytic cleavage of the colicin occurs only when the outer membrane barrier is permeabilized.
El Kouhen
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UPR 9027, IBSM-IFR1, Centre National de la Recherche Scientifique, Marseille, France.
Pagès
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D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003087:Colicins / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism
D003087:Colicins; D010450:Endopeptidases; D011188:Potassium