[Cryopreservation of isolates of Toxoplasma gondii in cell culture].
For a better preservation and identification of Toxoplasma gondii isolates, we propose a new method of freezing of toxoplasma growth in THP-1 cell culture. A cystogenic strain isolated from foetal blood has been grown in these cells and frozen in liquid nitrogen. After thawing, toxoplasma recover the same growth rate and morphology in vitro and the same capacity to form brain cysts into mice compared to the initial strains. The freezing of the cell suspension provides a simple and appropriate method for preservation of Toxoplasma gondii within "bank" isolates.
Cotty
F
F
Service de Parasitologie et Médecine Tropicale, Hôpital Bretonneau, Tours, France.
Descamps
P
P
Ferrer
A
A
Duong
T H
TH
Richard-Lenoble
D
D
D000818:Animals; D018929:Cell Culture Techniques; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D051379:Mice; D013997:Time Factors; D014122:Toxoplasma / Q000254:growth & development / Q000502:physiology; D014124:Toxoplasmosis, Animal; D016781:Toxoplasmosis, Cerebral
Cryopréservation d'isolats de Toxoplasma gondii en culture cellulaire.