Growth resistance-sized arteries in response to bladder hypertrophy in the rat: time-course, DNA-synthesis and LDH-isoform pattern.
Bladder growth was induced by partial urethral obstruction. Bladder hypertrophy was evident at 53 h after obstruction and continued over a 6 weeks period. Small bladder arteries were taken from fixed anatomical locations of the bladder circulation, mounted in a small vessel myograph and the optimal diameter for maximal isometric force development was determined (Lmax K+ = 125 mM stimulation). Bladder hypertrophy was associated with an enlarged Lmax from 53h onward (compared with sham-operated controls) and Lmax continued to increase until 10 days after urethral obstruction. Between 10 days and 6 weeks no further increase of the diameter was observed. Increased diameters in vitro were accompanied by a transiently increased [3H] Thymidine uptake in the small arteries which peaked at 53 h after obstruction but was still above background at 10 days. At this time point, small arterial growth was associated with a significant relative increase in the M isoform of LDH as determined with agarose electrophoresis on tissue homogenates. Thus organ growth induced small vessel growth in the rat is characterized by a rapid onset, increased but transient DNA-turnover and LDH-isoform changes. The latter mimic changes seen in other types of smooth muscle growth.
Boels
P J
PJ
Department of Physiology and Neuroscience, Lund University, Sweden.
Arner
A
A
Nilsson
B O
BO
Svensson
C
C
Uvelius
B
B
D000818:Animals; D001158:Arteries / Q000201:enzymology / Q000254:growth & development; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006984:Hypertrophy / Q000503:physiopathology; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008026:Ligation; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D001743:Urinary Bladder / Q000098:blood supply* / Q000473:pathology* / Q000601:surgery; D014555:Urination Disorders / Q000503:physiopathology
D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase