The effect of human calcitonin gene-related peptide on eosinophil chemotaxis in the rat airway.
Eosinophils play a key role for the function of release inflammatory mediators and destroy epithelial tissue in the airway. Therefore, they have been accepted to be proinflammatory effector cells in the pathogenesis of the bronchial asthma. The aim of this study was to investigate the effect of the calcitonin gene-related peptide (CGRP) a 37-amino acid neuropeptide on eosinophils responsible for hypersensitivity using BAL fluids that represent the cell population in the lung tissue. For this purpose, 15 rats were divided into three groups receiving vehicle, 10(-6) M and 10(-5) M CGRP using a portable nebulizer. Nebulated exposure of CGRP resulted in both significant increases in the eosinophil numbers with 10(-6) and 10(-5) M CGRP of 18 +/- 0.913 cell/mm2 (mean +/- SEM: p < 0.01) and 31.25 +/- 1.931 (p < 0.01), respectively vs. control (12 +/- 0.408 cell/mm2). CGRP is capable of causing a eosinophilia in the lung in vivo and may contribute to airway inflammation in patients with asthma.
Bellibaş
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Adnan Menderes University, School of Medicine, Department of Pharmacology, Aydm, Turkey.
D000704:Analysis of Variance; D000818:Animals; D001249:Asthma / Q000209:etiology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects*; D006801:Humans; D008297:Male; D051381:Rats; D012137:Respiratory System / Q000187:drug effects*
D015740:Calcitonin Gene-Related Peptide