title: [A new class of small RNP (alpha-RNP) containing antisense RNA in K-562 cells. II. The interaction of the RNA--the alpha-RNP component--with heterogeneous nuclear and messenger RNA in the normal state and under DMSO exposure].
abstract: Small antisense RNA (alpha-RNA), components of a new class of small nuclear and cytoplasmic RNP (alpha-RNP) identified in the cells of K-562 human proerythroleukemia cell line, are capable of hybridizing under stringent conditions with precursors of mRNA (heterogeneous nuclear RNA or mRNA) and with mRNA of these cells. We found that DMSO, an agent inducing differentiation in K-562 cells, is capable of regulating the composition of alpha-RNA population and concomitantly changes the content of mRNA that has regions homologous (complementary) to alpha-RNA. Specifically, it has been demonstrated that DMSO decreases the level of alpha-RNA, which hybridizes with the actin gene. Results of restriction mapping of regions of complementary interaction of alpha-RNA with the actin gene point out that alpha-RNA hybridizes with regions containing the promotor area and 3'-nontranslated area of the gene. It is proposed that small antisense alpha-RNA (alpha-RNP) participates in the control of gene expression at posttranscriptional level in cell cytoplasm.
authors: I M
authors: IM
authors: Konstantinova
authors: O A
authors: OA
authors: Petukhova
authors: V A
authors: VA
authors: Kulichkova
authors: I V
authors: IV
authors: Kozhukharova
authors: Iu B
authors: IuB
authors: Ermolaeva
authors: L N
authors: LN
authors: Gauze
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D016372:RNA, Antisense / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D012334:RNA, Neoplasm / Q000187:drug effects / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear / Q000187:drug effects / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D017411:Ribonucleoproteins, Small Nuclear; D004121:Dimethyl Sulfoxide
vernacular_title: Novyĭ klass malykh RNP (al'fa-RNP), soderzhashchikh antismyslovuiu RNK, v kletkakh linii K-562. II. Vzaimodeĭstvie RNK--komponenta al'fa-RNP--s geterogennymi iadernymi i informatsionnymi RNK v norme i pod vozdeĭstviem DMSO.