Immunohistochemical demonstration of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in cattle tissues.
Bromodeoxyuridine (BrdU), a non-radioactive thymidine analogue, was administered to 15 cattle at a dosage of 1-10 mg/kg intravenously or intraperitoneally to demonstrate S-phase cells in the tissues. The organs and tissues were fixed in 10% neutral buffered formalin or in 70% ethanol, sectioned, denatured with hydrochloric acid, and treated with monoclonal antibody against BrdU. Immunohistochemical methods were used to "visualize" BrdU-labelled nuclei. BrdU-positive cells were satisfactorily demonstrated in both formalin- and ethanol-fixed tissues of animals given doses of 2 mg/kg or over, by either route of administration. Large numbers of BrdU-positive cells indicative of active cell production were found in the basal region of the stratified squamous epithelium, the neck between gastric pits and gastric glands in the abomasum, and the crypts of Lieberkühn of the small and large intestines. Moderate numbers of positive cells were observed amongst inflammatory cells in cases of nephritis and in granulation tissue. Numerous positive cells were detected in leukaemia cells. The study showed that BrdU can be used to measure proliferative S-phase cells in cattle, as in human beings, mice and rats.
Yanai
T
T
Department of Veterinary Pathology, Gifu University, Tokagun, Japan.
Matsumoto
C
C
Takashima
H
H
Yoshida
K
K
Sakai
H
H
Isowa
K
K
Iwasaki
T
T
Sato
Y
Y
Masegi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001973:Bromodeoxyuridine / Q000032:analysis / Q000276:immunology; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics; D002418:Cattle Diseases / Q000235:genetics / Q000473:pathology; D003584:Cytological Techniques / Q000662:veterinary*; D004261:DNA Replication; D016583:Enzootic Bovine Leukosis / Q000235:genetics / Q000473:pathology; D005260:Female; D005821:Genetic Techniques / Q000662:veterinary*; D008297:Male; D009393:Nephritis / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D016196:S Phase; D014018:Tissue Distribution; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine