Promoter usage determines tissue specific responsiveness of the rat acetyl-CoA carboxylase gene.
The acetyl-CoA carboxylase gene contains two promoters, PI and PII which generate multiple mRNA forms. We have used the reverse transcription-polymerase chain reaction to investigate tissue specific promoter usage in rats either fed a standard chow diet, starved for 48 h, or starved and then refed a high carbohydrate, low fat diet. Expression of PII-generated mRNAs was seen in all tissues examined and was not dramatically changed by food removal or refeeding. PI-generated mRNAs were expressed at variable levels in a narrower range of tissues and were regulated by these dietary manipulations. Thus only the PI promoter is responsive to diet and the ability of a tissue to use this promoter determines whether it can alter fatty acid synthesis in response to nutritional challenges.
Kim
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TS
Department of Nutritional Sciences, University of Connecticut, Storrs 06269-4017, USA.
Leahy
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P
Freake
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HC
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D012333:RNA, Messenger; D000103:Acetyl-CoA Carboxylase