EM colocalization of AMPA and NMDA receptor subunits at synapses in rat cerebral cortex.
Electrophysiology and light microscopy suggest that a single excitatory synapse may use both amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors. Using immunogold electron microscopy, we here provide direct evidence for colocalization at individual synapses in sensorimotor cortex of adult rats. Colocalization was most commonly observed on dendritic spines; subunits of the two classes of receptors seemed to be independently distributed within the synaptic active zone.
Kharazia
V N
VN
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599, USA.
Phend
K D
KD
Rustioni
A
A
Weinberg
R J
RJ
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D003712:Dendrites / Q000737:chemistry / Q000648:ultrastructure; D018698:Glutamic Acid / Q000502:physiology; D017979:Gold Colloid; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000648:ultrastructure*; D016194:Receptors, N-Methyl-D-Aspartate / Q000648:ultrastructure*; D013569:Synapses / Q000737:chemistry* / Q000648:ultrastructure
D017979:Gold Colloid; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid