title: Second transplantation with CD34+ bone marrow cells selected from a two-loci HLA-mismatched sibling for a patient with chronic myeloid leukaemia.
abstract: A 43-year-old man with chronic myeloid leukaemia underwent a second transplant with CD34+ bone marrow cells selected from his two-loci HLA-mismatched sibling after rejection of the first graft from an HLA-matched unrelated donor. By immunomagnetic positive selection, CD34+ marrow cells at 0.95 x 10(6)/kg with 97% purity and CD3+ T lymphocytes at 1.3 x 10(4)/kg were collected and transplanted. Engraftment was confirmed to be of CD34+ cell-donor origin. The patient developed only grade I acute graft-versus-host disease (GVHD) and no chronic GVHD to date. These observations suggest that allogeneic CD34+ bone marrow cells are capable of reconstituting haemopoiesis and that CD34+ selection could be applicable to T-cell depletion.
authors: Second Department of Internal Medicine, Hyogo College of Medicine, Japan.
authors: Y
authors: Y
authors: Fujimori
authors: A
authors: A
authors: Kanamaru
authors: N
authors: N
authors: Hashimoto
authors: T
authors: T
authors: Okamoto
authors: M
authors: M
authors: Okada
authors: K
authors: K
authors: Kawaguchi
authors: A
authors: A
authors: Mori
authors: K
authors: K
authors: Saheki
authors: H
authors: H
authors: Takatsuka
authors: H
authors: H
authors: Wada
authors: Y
authors: Y
authors: Takemoto
authors: M
authors: M
authors: Kohsaki
authors: N
authors: N
authors: Imai
authors: E
authors: E
authors: Kakishita
authors: K
authors: K
authors: Nagai
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000379:methods*; D006084:Graft Rejection; D006086:Graft vs Host Disease / Q000209:etiology / Q000517:prevention & control; D006650:Histocompatibility Testing; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy*; D008231:Lymphopenia / Q000209:etiology; D008297:Male; D013601:T-Lymphocytes
chemical_list: D018952:Antigens, CD34; D000961:Antilymphocyte Serum