Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.
Expressed sequence tags (ESTs) from 298 clones have been generated from a randomly primed, normalized human adult thymus cDNA library. We describe the chromosomal localization of 136 of these ESTs by PCR-based mapping to a human monochromosomal somatic cell hybrid panel. Data base similarities to known genes are also described. A subset (n = 18) of these randomly primed ESTs extended the sequence of ESTs from other tissues currently in dbEST. Of the nonrepetitive human adult thymus ESTs generated in this study, 237 (79.5%) have no similarity to current data base entries. This would suggest that our collection contains approximately 100 new coding regions from thymus tissue, a large proportion of which likely will represent the middle regions of genes. The mapped ESTs should prove useful as new gene-based markers for mapping and candidate gene hunting, particularly when anchored to a well-developed physical map of the human genome.
Lamerdin
J E
JE
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550, USA. lamerdin@llnl.gov
Athwal
R S
RS
Kansara
M S
MS
Sandhu
A K
AK
Patanjali
S R
SR
Weissman
S M
SM
Carrano
A V
AV
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D016208:Databases, Factual; D015870:Gene Expression; D015723:Gene Library; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D013950:Thymus Gland / Q000737:chemistry*
D018076:DNA, Complementary; D005819:Genetic Markers