Determination of the terfenadine metabolite azacyclonol in human serum using gas chromatography-mass spectrometry.
In this work, a method for the determination of azacyclonol, a primary metabolite of terfenadine, in human serum is described. Sample preparation is carried out by liquid-liquid extraction under basic conditions. For an efficient clean up, the analytes are back-extracted into diluted hydrochloric acid and, after alkalinization, are once again extracted into the organic phase. No derivatisation step is performed. The samples are measured by gas chromatography-mass spectrometry with good selectivity. The limit of detection is 2 ng/ml. The coefficients of variation are 12.6% at 10 ng/ml and 6.44% at 200 ng/ml in the day-to-day control measurements.
Martens
J
J
Otto-von-Guericke-Universität Magdeburg, Medizinische Fakultät, Inst. f. Klinische Pharmakologie, Germany.
D014151:Anti-Anxiety Agents / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D010880:Piperidines / Q000097:blood*; D012680:Sensitivity and Specificity; D016593:Terfenadine / Q000378:metabolism*
D014151:Anti-Anxiety Agents; D010880:Piperidines; C100095:azacyclonol; D016593:Terfenadine