Construction of a single-chain Fv from an antibody which catalyses a Diels Alder cycloaddition.
Brooks
L
L
Department of Immunology, University of Strathclyde, Glasgow, Scotland.
Suckling
C J
CJ
Stimson
W H
WH
D000085:Acetates / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli; D005033:Ethylmaleimide / Q000737:chemistry*; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008301:Maleimides / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins
D000085:Acetates; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008301:Maleimides; D011994:Recombinant Proteins; C102107:N-benzylmaleimide; D005033:Ethylmaleimide