RANTES chemokine expression in diseased and normal human tissues.
RANTES is a member of a large family of cytokines, called chemokines, which are thought to play a regulatory role in inflammatory processes. We have made recombinant human RANTES protein which was used to generate a panel of anti-RANTES monoclonal antibodies. Following characterization, select anti-RANTES monoclonal antibodies were used for immunohistologic staining of a large panel of normal, diseased and fetal tissue sections. Diseased tissues included eleven lymphomas and eight renal tumors. Most tissues were also tested in parallel for RANTES mRNA by in situ hybridization using RANTES mRNA specific oligomeric probes. As expected, most normal adult tissues contain few, if any, RANTES positive cells. In contrast, RANTES expression dramatically increases in inflammatory sites. In addition, megakaryocytes, some tumours, and select fetal tissues express high levels of RANTES message and protein. These results indicate a wider expression of RANTES than previously appreciated and suggest multiple physiologic roles for this soluble factor.
von Luettichau
I
I
Department of Pediatrics, Stanford University Medical Center, California 94305, USA.
Nelson
P J
PJ
Pattison
J M
JM
van de Rijn
M
M
Huie
P
P
Warnke
R
R
Wiedermann
C J
CJ
Stahl
R A
RA
Sibley
R K
RK
Krensky
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5 / Q000096:biosynthesis* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D005260:Female; D005333:Fetus; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007938:Leukemia / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D014066:Palatine Tonsil / Q000276:immunology; D011247:Pregnancy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D012016:Reference Values; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5; D011994:Recombinant Proteins