[Modulation of bcl-2 antisense RNA on programmed cell death of leukemic cell line].
OBJECTIVE
To investigate modulation of decrease of intrinsic bcl-2 protein levels on programmed cell death of leukemia cells.


METHODS
Gene transfection procedure was applied to observe the effect of antisense RNA-mediated suppression of bcl-2 gene expression on programmed cell death of human T-lymphocytic leukemia cell line CEM.


RESULTS
Temporary expression of antisense bcl-2 gene could effectively reduce levels of intrinsic bcl-2 protein of CEM cells and render it more sensitive to etoposide-induced cytotoxicity. Moreover, a great deal of apoptotic bodies and ladder DNA was always produced during etoposide-mediated killing of CEM and when CEM expressing bcl-2 antisense RNA served as target cells in particular, the amount of ladder DNA increased to around 40%.


CONCLUSIONS
Programmed cell death is one of the mechanisms by which etoposide kills leukemic cells and is modulated by cellular intrinsic bcl-2 protein.
Chen
X
X
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Wang
W
W
Huang
G
G
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2; D016372:RNA, Antisense; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016372:RNA, Antisense; D005047:Etoposide