Identification of a 25-kilodalton protein of Mycobacterium bovis BCG to distinguish BCG strains from Mycobacterium tuberculosis.
Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.
Kumar
D
D
Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
Srivastava
B S
BS
Singh
N B
NB
Srivastava
R
R
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D001500:BCG Vaccine / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016867:Immunocompromised Host; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D014376:Tuberculosis / Q000209:etiology / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate