CD45 isoforms on human CD4+ T-cell subsets.
We have examined the level of surface expression and functional properties of leukocyte function associated antigen-1, very late antigen-4, and CD45 isoforms on a panel of human CD4+ T-cell clones representative of TH0, TH1, and TH2 cells. There were no qualitative differences in the expression of these antigens among the three types of CD4+ T-cell clones. However, CD45RB was the only CD45 isoform that provided a costimulatory signal in a solid-phase antibody-induced cellular proliferation assay. Additionally, the antigen-induced proliferative response of T-cell clones was inhibited by soluble anti-CD45RO and anti-CD45RB antibodies. Our results suggest that CD45 isoforms differentially provide costimulatory signals to T cells. However, the ability of each CD45 isoform to provide a costimulatory signal does not differ among the TH0, TH1, or TH2 T-cell populations.
Shanafelt
M C
MC
Department of Leukocyte Biology, Syntex Research (Roche Bioscience), Palo Alto, California, USA.
Yssel
H
H
Soderberg
C
C
Steinman
L
L
Adelman
D C
DC
Peltz
G
G
Lahesmaa
R
R
D015496:CD4-Positive T-Lymphocytes / Q000145:classification / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D002999:Clone Cells; D006801:Humans; D017493:Leukocyte Common Antigens / Q000145:classification* / Q000378:metabolism / Q000502:physiology; D008213:Lymphocyte Activation; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D017493:Leukocyte Common Antigens