Stimulation via the CD43 coreceptor augments T cell proliferation during the early phase of antigen-induced activation.
R2/60 is a monoclonal antibody (mAb) that recognizes the murine CD43 molecule expressed on mature T cells, developing thymocytes, and a subset of bone marrow hematopoietic stem cells. Recent studies using R2/60 demonstrate that CD43 is a costimulatory receptor involved in the activation of murine T cells. In the present study we have examined the kinetics of CD43-mediated costimulation in murine T cell populations including purified CD4+ and CD8+ peripheral T cells, and T cells activated in antigen-induced mixed-lymphocyte cultures. In each population, CD43 stimulation significantly enhanced T cell proliferation compared to non-CD43 activated cultures. CD43 costimulation was greatest in conjunction with suboptimal CD3 stimulation, and was most efficient during the early phase of antigen-driven activation, suggesting that under normal biologic conditions the role of CD43 may be to augment T cell responses early in immune activation.
Hamad
M
M
Department of Biological Science, University of Tulsa, OK 74104, USA.
Mosley
R L
RL
Wang
J
J
Klein
J R
JR
D000818:Animals; D015703:Antigens, CD; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D051917:Leukosialin; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D017252:CD3 Complex; D051917:Leukosialin; D011948:Receptors, Antigen, T-Cell; D012795:Sialoglycoproteins; C497863:Spn protein, mouse