Lack of effect of cocaine on lysine and alanine uptake in human placental villi or transfer in perfused human placenta.
The effect of cocaine on lysine and alanine uptake in human placental villi and transfer across the dually perfused placenta was studied. Uptake (in terms of the intracellular to extracellular distribution ratio) of alanine and lysine was 2.81 +/- 0.30 (n = 5) and 1.45 +/- 0.24 (n = 5) respectively and was unaffected by cocaine (50-500 ng mL(-1) in the incubation medium. In the dually perfused placenta, the clearance index (ratio of amino acid to antipyrine clearance) was 0.35 +/- 0.03 and 0.30 +/- 0.05 and the transfer index (ratio of amino acid to L-glucose clearance) was 2.20 +/- 0.07 and 1.89 +/- 0.29 for lysine and alanine respectively. Cocaine at concentrations of 100 ng mL(-1) or 250 ng mL(-1) had no effect on the clearance of either amino acid. The results of this study indicate that concentrations of cocaine likely to be encountered in vivo do not affect uptake of lysine or alanine by placental villi or transfer across the perfused placental lobule, in contrast with the report that cocaine reduces uptake of alanine by placental vesicles. Experimental models must be critically evaluated before accepting the results as pertinent to a clinical situation.
Krishna
R B
RB
Department of Obstetrics and Gynecology, New York University Medical Center, New York 10016, USA.
Levitz
M
M
Dancis
J
J
D000409:Alanine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002824:Chorionic Villi / Q000187:drug effects* / Q000378:metabolism; D003042:Cocaine / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy
D003042:Cocaine; D008239:Lysine; D000409:Alanine