Differentiation of African horse sickness viruses by polymerase chain reaction and segments 10 restriction patterns.
This paper describes a single tube reverse transcription-polymerase chain reaction (RT-PCR) method for detection of African horse sickness virus (AHSV). The genomic segments 10 of viruses of the 9 AHSV serotypes were amplified. The 758bp products were digested to completion by restriction enzymes. The restriction fragment length polymorphisms of segments 7 and 10 cDNA allowed the differentiation of the nine serotypes.
Zientara
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S
Centre National d'Etudes Vétérinaires et Alimentaires Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
Sailleau
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C
Moulay
S
S
Cruciere
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C
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006734:Horse Diseases; D006736:Horses; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012087:Reoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D015183:Restriction Mapping
D017931:DNA Primers; D018076:DNA, Complementary