title: ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae.
abstract: The RHO1 gene encodes a homolog of the mammalian RhoA small GTP-binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth site and is required for bud formation. The RHO1(G22S, D125N) mutation is a temperature-sensitive and dominant negative mutation of RHO1, and a multicopy suppressor of RHO1(G22S, D125N), ROM7, was isolated. Nucleotide sequencing of ROM7 revealed that it is identical to the BEM4 gene (GenBank accession number L27816), although its physiological function has not yet been reported. Disruption of BEM4 resulted in the cold- and temperature-sensitive growth phenotypes, and cells of the deltabem4 mutant showed abnormal morphology, suggesting that BEM4 is involved in the budding process. The temperature-sensitive growth phenotype was suppressed by overexpression of RHO1, ROM2, which encodes a Rho1p-specific GDP/GTP exchange factor, or PKC1, which encodes a target of Rho1p. Moreover, glucan synthase activity, which is activated by Rho1p, was significantly reduced in the deltabem4 mutant. Two-hybrid and biochemical experiments revealed that Bem4p directly interacts with the nucleotide-free form of Rho1p and, to lesser extents, with the GDP- and GTP-bound forms of Rho1p, although Bem4p showed neither GDP/GTP exchange factor, GDP dissociation inhibitor, nor GTPase-activating protein activity toward Rho1p. These results indicate that Bem4p is a novel protein directly interacting with Rho1p and is involved in the RHO1-mediated signaling pathway.
authors: Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan.
authors: H
authors: H
authors: Hirano
authors: K
authors: K
authors: Tanaka
authors: K
authors: K
authors: Ozaki
authors: H
authors: H
authors: Imamura
authors: H
authors: H
authors: Kohno
authors: T
authors: T
authors: Hihara
authors: T
authors: T
authors: Kameyama
authors: K
authors: K
authors: Hotta
authors: M
authors: M
authors: Arisawa
authors: T
authors: T
authors: Watanabe
authors: H
authors: H
authors: Qadota
authors: Y
authors: Y
authors: Ohya
authors: Y
authors: Y
authors: Takai
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000502:physiology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D005656:Fungal Proteins / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D005964:Glucosyltransferases / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D011485:Protein Binding; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins
chemical_list: C100926:BEM4 protein, S cerevisiae; D002352:Carrier Proteins; D017931:DNA Primers; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D005964:Glucosyltransferases; C040232:glucan synthase; D019204:GTP-Binding Proteins; C051302:RHO1 protein, S cerevisiae; D020741:rho GTP-Binding Proteins