title: Genetic analysis of factors controlling high-level expression of cytochrome P450, CYP6D1, cytochrome b5, P450 reductase, and monooxygenase activities in LPR house flies, Musca domestica.
abstract: To understand better the biochemical genetics of cytochrome P450 monooxygenase-mediated insecticide resistance, we examined the microsomal monooxygenases in insecticide-susceptible (aabys) and pyrethroid-resistant (LPR) house fly strains, as well as 15 house fly lines derived from crosses of LPR and aabys. In comparison to the aabys strain, LPR had higher levels of total cytochromes P450, cytochrome b5, P450 reductase, CYP6D1, and three P450 monooxygenase activities: 7-ethoxycoumarin O-deethylase (ECOD), methoxyresorufin O-demethylase (MROD), and aromatic hydrocarbon hydroxylase (AHH). The elevated levels of cytochrome b5 were linked to factors on autosomes 1 and 2. This is similar to previous reports on monooxygenase-mediated resistance and is consistent with the idea that elevated cytochrome b5 levels are involved in monooxygenase-mediated resistance in the LPR strain. Linkage of the elevated P450 reductase is different from that of monooxygenase-mediated resistance. Strains having high levels of CYP6D1 (i.e., like LPR) had high levels of P450 reductase, while strains having intermediate levels of CYP6D1 also had high levels of reductase. Therefore, there is no clear evidence that the elevated P450 reductase in the LPR strain is required for the increased monooxygenase activity. Overexpression of total cytochromes P450, CYP6D1 (mRNA and protein), and CYP6D1-mediated monooxygenase activities (MROD and AHH) in LPR microsomes was linked to a combination of factors on autosomes 1 and 2. This demonstrates that increased expression of CYP6D1 in the LPR strain is both cis regulated by a factor(s) on autosome 1 and trans regulated by a factor(s) on autosome 2. The correlation between the overexpression of CYP6D1 mRNA and protein suggests that CYP6D1 expression is regulated transcriptionally. Monooxygenase-mediated resistance in LPR is controlled by factors on autosomes 1 and 2, which supports previous claims that CYP6D1 is responsible for monooxygenase-mediated resistance in the LPR strain.
authors: Department of Entomology, Cornell University, Ithaca, New York 14853-0901, USA.
authors: N
authors: N
authors: Liu
authors: J G
authors: JG
authors: Scott
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D000072472:Cytochrome P450 Family 6; D003580:Cytochromes / Q000235:genetics*; D015786:Cytochromes b5 / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006793:Houseflies / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D019476:Insect Proteins; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics; D010105:Oxygenases / Q000235:genetics; D011722:Pyrethrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D003580:Cytochromes; D019476:Insect Proteins; D007306:Insecticides; D011722:Pyrethrins; D012333:RNA, Messenger; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; C092307:CYP6D1 protein, Musca domestica; D000072472:Cytochrome P450 Family 6; D009251:NADPH-Ferrihemoprotein Reductase