4-Pentafluoroethylumbelliferyl-beta-D-glucoside as a new fluorogenic substrate for acid beta-D-glucosidase.
4-Pentafluoroethylumbelliferyl-beta-D-glucoside is proposed as an efficient substrate for human leukocyte acid beta-glucosidase. Its synthesis is described. This substrate was compared directly with 4-trifluoromethylumbelliferyl-beta-D-glucoside synthesized by us earlier and with 4-methylumbelliferyl-beta-D-glucoside which is commonly used for acid beta-glucosidase activity assay. The specific activity of acid beta-glucosidase with 4-pentafluoroethylumbelliferyl-beta-D-glucoside was 3- and 8-fold higher than it was with the substrates mentioned above. The kinetic parameters KM and VMAX for human leukocyte acid beta-glucosidase with the three substrates was determined. One possible application of the newly synthesized substrate is its use in the diagnosis of acid beta-glucosidase hereditary deficiency (Gaucher's disease).
Tsvetkova
I V
IV
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Karpova
E A
EA
Dudukina
T V
TV
Voznyi
Y V
YV
D005456:Fluorescent Dyes; D005776:Gaucher Disease / Q000201:enzymology; D005960:Glucosides / Q000138:chemical synthesis / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000737:chemistry; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013053:Spectrophotometry; D014468:Umbelliferones / Q000138:chemical synthesis / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
C102826:4-pentafluoroethylumbelliferylglucoside; D005456:Fluorescent Dyes; D005960:Glucosides; D014468:Umbelliferones; D006923:Hymecromone; D001617:beta-Glucosidase