Destruction of human immunodeficiency-infected cells by ferrofluid particles manipulated by an external magnetic field: mechanical disruption and selective introduction of cytotoxic or antiretroviral substances into target cells.
Submagnetic domain magnetic fluid particles of approximately 10 nm average diameter complexed with CD4 or monoclonal antibody and then injected into the patient, will localize to the cell membrane of the target cell. These ferrofluid particles will interact with an externally applied rotating magnetic field of rapidly changing polarity. Under these conditions, the ferrofluid particles will be drawn into a circular path and an axial spin will be induced as each particle aligns itself with the magnetic force lines. A portion of these magnetic fluid particles will be drawn into the target cell membrane and into the cytoplasm causing brief perforations of the cell membrane of the target cells. If enough mechanical damage is done to the plasma membrane or to the intracellular structures, cell lysis may result, but in any case the brief disruptions of the target cell membrane can be used to selectively introduce membrane impermeant cytotoxic or antiretroviral substances into the target cell while relatively sparing normal cells.
Peasley
K W
KW
D000818:Animals; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents / Q000633:toxicity*; D015704:CD4 Antigens / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006678:HIV / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D015658:HIV Infections / Q000628:therapy*; D006801:Humans; D007501:Iron; D008280:Magnetics; D008954:Models, Biological
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D015704:CD4 Antigens; D007501:Iron