[Evaluation and its clinical significance of anti-platelet granule membrane protein-140 autoantibodies and anticalmodulin antibody in patients with severe pregnancy-induced hypertension].
OBJECTIVE
To study the effect of immune function of platelet on the pathogenesis of severe pregnancy-induced hypertension.


METHODS
46 plasma samples from patients with severe pregnancy-induced hypertension (PIH) were evaluated for autoantibodies directed against granule membrane protein-140 (GMP-140) and anticalmodulin antibody (A-CaM) using ELISA methods.


RESULTS
Autoantibodies to GMP-140 existed in 13 patients with severe PIH (28.6%), after SDS-page of purified GMP-140 immunoblotting showed that 1 out of 8 sera from patients presented a staining band with M.W. of 140 kd. 29 plasma samples were found autoantibodies to GMP-140, GP IIb/IIIa and GP Ib/IX, simultaneously. It was shown that antiGMP-140 activities coexisted with anti-GP IIb/IIIa and/or anti-GPIb/Ix in 29 cases (81.8%). The elevated A-CaM was observed in the patients with severe PIH reaching to 20.02 +/- 3.74U/L, that was much higher than those from the normal (7.53 +/- 2.57U/L, P < 0.05).


CONCLUSIONS
Our study provided the evidence for autoantibodies to GMP-140 and A-CaM in some of patients with severe PIH, it has certain importance for further study on the pathogenesis of severe PIH.
Zhang
S
S
Affiliated Hospital of Shandong Medical University, Jinan.
Dong
J
J
Liu
B
B
D001323:Autoantibodies / Q000097:blood*; D002147:Calmodulin / Q000276:immunology*; D005260:Female; D006801:Humans; D019007:P-Selectin / Q000276:immunology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy
D001323:Autoantibodies; D002147:Calmodulin; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex