title: Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2.
abstract: Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.
authors: Department of Plant Biology, Louisiana State University, Baton Rouge 70803, USA.
authors: M D
authors: MD
authors: Burow
authors: Z Y
authors: ZY
authors: Chen
authors: T M
authors: TM
authors: Mouton
authors: J V
authors: JV
authors: Moroney
mesh_terms: D000818:Animals; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D002245:Carbon Dioxide