title: Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles.
abstract: Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 micrograms/ml) from the same sera, not containing beta 2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
authors: Departamento de Fármacos e Medicamentos, Universidade Estadual Paulista, Araraquara, SP, Brasil.
authors: M P
authors: MP
authors: Gremião
authors: C M
authors: CM
authors: Celli
authors: H
authors: H
authors: Chaimovich
mesh_terms: D017153:Antibodies, Anticardiolipin / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002308:Cardiolipins / Q000276:immunology* / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D015388:Organelles / Q000378:metabolism*; D013587:Syphilis / Q000276:immunology*
chemical_list: D017153:Antibodies, Anticardiolipin; D002308:Cardiolipins; D005452:Fluoresceins; C024098:6-carboxyfluorescein