Effects of egtazic acid and calcimycin on synthesis of DNA and collagen in cultured human lung fibroblasts.
OBJECTIVE
To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF).


METHODS
The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively.


RESULTS
The collagen synthesis increased markedly 24 h after exposure to both EA (0.05-4 mmol.L-1) and Cal (0.25-20 mumol.L-1), and that there was no obvious change in DNA synthesis. After 36-48-h exposure, both DNA and collagen syntheses decreased in the groups of EA (1, 2, and 4 mmol.L-1); the DNA synthesis was also suppressed in Cal groups in a concentration-dependent manner, whereas collagen synthesis decreased only in Cal (10 and 20 mumol.L-1).


CONCLUSIONS
Extracellular Ca2+ influx into fibroblasts increased collagen production, However, the DNA synthesis was suppressed when the cytosolic Ca2+ was too high or too low.
Liu
H L
HL
Department of Internal Medicine, Xin-Hua Hospital, Shanghai Second Medical University, China.
Lu
H M
HM
Li
D G
DG
Qi
F
F
Jiang
Z M
ZM
D001693:Biological Transport, Active; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D004533:Egtazic Acid / Q000494:pharmacology*; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D008168:Lung / Q000166:cytology
D002614:Chelating Agents; D007476:Ionophores; D000001:Calcimycin; D004533:Egtazic Acid; D003094:Collagen; D004247:DNA; D002118:Calcium