Identification and sequence comparison of a cuticular collagen of Brugia pahangi.
The cuticle of filarial nematodes is a specialized extracellular matrix that covers the parasite and protects it from adverse conditions of the environment. As a surface structure it is in direct contact with the host defence mechanisms and therefore plays an important role in the molecular host-parasite relationship. Using polyclonal antisera raised against the insoluble components of the cuticle of the adult filarial parasite Brugia pahangi, we have isolated cDNA clones encoding collagen molecules of the cuticle. The protein domain structure of cDNA clone Bpcol-1 was compared with the known structures of cuticular collagens of the nematodes Brugia malayi, Caenorhabditis elegans, Ascaris suum and Haemonchus contortus, confirming interspecies similarities. Using affinity-purified anti-Bpcol-1 antibodies we identified Bpcol-1 antigenic determinants in different nematode extracts, and determined the localization of such epitopes within the cuticle of B. pahangi.
Bisoffi
M
M
Department of Medical Parasitology, Swiss Tropical Institute, Basel, Switzerland.
Betschart
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B
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth; D017179:Brugia pahangi / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003094:Collagen / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D000939:Epitopes / Q000032:analysis; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D017433:Protein Structure, Secondary; D018510:RNA, Helminth / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000947:Antigens, Helminth; C101010:COL-1 protein, Brugia pahangi; D018076:DNA, Complementary; D018509:DNA, Helminth; D000939:Epitopes; D015801:Helminth Proteins; D018510:RNA, Helminth; D003094:Collagen