Very low density lipoprotein and oxidized very low density lipoprotein induce monocyte chemotactic protein 1 in rabbit aortic smooth muscle cells.
OBJECTIVE
To understand whether very low density lipoprotein (VLDL) and oxidatively modified very low density lipoprotein (OX-VLDL) can stimulate vascular smooth muscle cells (SMCs) to express high level of monocyte chemotactic protein (MCP-1) mRNA.


METHODS
LDL and VLDL were isolated from normal blood donors by density gradient ultracentrifugation and oxidatively modified by adding CuCl2 and incubation at 37 degrees C for 24 hours. Rabbit aortic SMCs were cultured by a substrate-attached explant method, and their total RNA was extracted by guanidinium isothiocyanate method. The expression of MCP-1 mRNA in SMCs was examined by dot blotting analysis using a probe of gamma-22P-end-labeled 35-mer oligonucleotide.


RESULTS
Dot blotting analysis showed that cultured SMCs can express MCP-1 mRNA and the level of MCP-1 mRNA was increased significantly in SMCs after 24-hour coculture with VLDL (6-fold) and OX-VLDL (20-fold).


CONCLUSIONS
VLDL and OX-VLDL both can induce MCP-1 mRNA expression in cultured rabbit aortic SMCs and the latter is more effective than the former.
Ruan
Q
Q
Department of Pathology, Tongji Medical University, Wuhan.
Deng
Z
Z
Song
J
J
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001483:Base Sequence; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D015870:Gene Expression; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits
D018932:Chemokine CCL2; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger