The 66-kDa neurofilament protein (NF-66): sequence analysis and evolution.
A 2.5 kb cDNA clone encoding the mouse 66 kd neurofilament protein (NF-66) was isolated and sequenced. The deduced protein sequence contains 501 amino acid residues. Comparison of the mouse, rat and human NF-66 indicated > 90% homology in protein sequence and 85% homology in coding nucleotide sequence. A high degree of homology was observed between NF-66 and other intermediate filament proteins especially in the alpha-helical domain. Zooblot analyses suggested that the putative ancestral gene for vimentin and NF-66 was detectable in the avian. By comparison, the ancestral sequence for GFAP appeared after that for vimentin.
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Saul Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Chiu
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014746:Vimentin / Q000235:genetics
D002352:Carrier Proteins; D018076:DNA, Complementary; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D014746:Vimentin; C046643:alpha-internexin