Multidrug resistant tuberculosis outbreak in Buenos Aires. DNA fingerprinting analysis of isolates.
In order to determine the possible relationship among HIV patients coinfected with multidrug resistant tuberculosis strains who had been receiving clinical assistance in our Hospital, clinical and epidemiological information from 28 patients was collected. DNA fingerprinting by restriction fragment length polymorphism (RFLP) pattern was performed on the mycobacterial isolates from these patients, using the restriction enzyme Pvull and IS 6110 as genetic marker. A unique RFLP pattern was found in 10 isolates from 10 different patients who had a disease caused by a single strain. Our findings confirm RFLP as a reliable and useful tool to analyze TB transmission.
Morcillo
N
N
Hospital Dr. A. Centrángolo, Vicente López, Buenos Aires.
Alito
A
A
Romano
M I
MI
Cataldi
A
A
Dolmann
A
A
Reniero
A
A
de Kantor
I N
IN
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology; D000328:Adult; D001118:Argentina / Q000453:epidemiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007538:Isoniazid; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin; D018088:Tuberculosis, Multidrug-Resistant / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*
D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin