Expression of the Sclerotinia sclerotiorum polygalacturonase pg1 gene: possible involvement of CREA in glucose catabolite repression.
Northern-blot analysis of RNA isolated from Sclerotinia sclerotiorum grown on either glucose or polygalacturonate as the sole carbon source showed that pg1, encoding a neutral polygalacturonase, was not expressed during growth in both media. In contrast, transcripts of this gene were detected during infection of sunflower germlings. Analysis of the promoter sequence revealed a number of cis-acting sequences known to regulate the expression of many fungal promoters. Protein-DNA-binding experiments showed that proteins extracted from mycelia grown on polygalacturonate or glucose interacted with different regions of the promoter. The GST-CREA fusion protein, containing the two zinc fingers of the Aspergillus nidulans repressor CREA involved in carbon catabolite repression, forms several complexes with DNA fragments carrying the consensus 5'-SYGGRG-3'. These results suggest that a CREA homolog may be involved in the regulation of pg1.
Reymond-Cotton
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Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 106, Université Lyon I, 43 bd 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
Fraissinet-Tachet
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D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics*; D001233:Aspergillus nidulans / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D016384:Consensus Sequence; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015183:Restriction Mapping
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C071237:CreA protein, Aspergillus nidulans; D005982:Glutathione Transferase; D011096:Polygalacturonase; D005947:Glucose