Presence of calmodulin and NADPH-diaphorase activity but not of neuronal nitric oxide synthase in the subcommissural organ of the Mongolian gerbil.
The subcommissural organ (SCO) and the ventricular ependyma of the third ventricle of the Mongolian gerbil (Meriones unguiculatus) exhibit a highly positive histochemical reaction to NADPH-diaphorase and a considerable immunocytochemical expression of calmodulin. The SCO, however, does not display an immunocytochemical response to the neuronal isoform of nitric oxide synthase (NOS) whereas it was demonstrated in the ventricular ependyma. Since the NADPH-diaphorase, which detects all isoforms of NOS, was clearly positive at the level of the SCO, the obtained results suggest that the SCO of the Mongolian gerbil produces nitric oxide but contains a NOS whose antigenic specificity differs from that of the neuronal isoform of this enzyme.
Krstic
R
R
Institute of Histology and Embryology, University of Lausanne, Switzerland.
Nicolas
D
D
D000818:Animals; D002147:Calmodulin / Q000032:analysis*; D005260:Female; D005849:Gerbillinae; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000032:analysis*; D013351:Subcommissural Organ / Q000737:chemistry* / Q000201:enzymology
D002147:Calmodulin; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase