title: Parainfluenza-3-virus infection enhances allergic sensitization in the guinea-pig.
abstract: BACKGROUND
  Viral respiratory tract infections have been previously considered to be associated with induction of allergic sensitization.
  
  
  OBJECTIVE
  In order to investigate this relationship in an animal model, guinea-pigs were inoculated intranasally with Parainfluenza-3-(PI-3) virus (n = 16) or virus-free culture medium (controls, n = 12), sensitized at day 4 with inhaled ovalbumin (OA) and challenged 3 weeks later with inhaled OA using specific bronchial provocation testing with body plethysmographic measurement of compressed air (CA). Furthermore, specific anti-OA-IgG1-antibodies in serum before challenge were determined by enzyme linked immunosorbent assay (ELISA). For investigation of airway epithelium permeability horseradish peroxidase (HRP) was inhaled at day 4 after inoculation by six animals, and HRP serum concentrations were determined by a direct ELISA 30 min after inhalation.
  
  
  RESULTS
  PI-3 infected animals were found to be significantly more sensitized to OA compared with controls, with higher CA values (P < 0.001) on specific bronchial provocation and with increased specific anti-OA-IgG1 titers. Serum-HRP concentrations were about 20 times higher in the infected animals compared with controls. PI-3 infected and sham-infected animals had comparable bronchial reactions on specific provocation with OA when sensitized systemically.
  
  
  CONCLUSIONS
  We conclude that viral respiratory tract infection with PI-3 virus enhances inhalative allergic sensitization in the guinea-pig. Increased mucosal permeability to antigens may be an important pathophysiological mechanism.
authors: Department of Paediatrics, Ruhr-University Bochum, Germany.
authors: F
authors: F
authors: Riedel
authors: A
authors: A
authors: Krause
authors: W
authors: W
authors: Slenczka
authors: C H
authors: CH
authors: Rieger
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000280:Administration, Inhalation; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods*; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000150:complications*
chemical_list: D000276:Adjuvants, Immunologic; D010047:Ovalbumin