[Diagnosis of Babesia bigemina with the immunoperoxydase test].
An immunoperoxidase assay for the serological diagnosis of Babesia bigemina was developed. The antigen slides were prepared from B. bigemina-infected blood and stored at -20 degrees C. One hundred and sixty five sera were tested, comparing the immunoperoxidase assay to the indirect fluorescent antibody test. A coincidence of 95% was observed between both tests. For the immunoperoxidase assay, a relative sensitivity of 94.8%, a relative specificity of 95.5%, and a positive predictive value of 96.8% were calculated. The results demonstrated the efficacy of this technique for detecting antibodies to B. bigemina.
Blandino
T
T
Laboratorio de Parasitología, Centro Nacional de Sanidad Agropecuaria, La Havane, Cuba.
Barrera
M
M
Alonso
M
M
Faure
M
M
Rodriguez Diego
J G
JG
D000818:Animals; D001404:Babesiosis / Q000175:diagnosis* / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D007124:Immunoenzyme Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
Diagnostic de Babesia bigemina avec le test à l'immunoperoxydase.