Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2.
Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.
Burow
M D
MD
Department of Plant Biology, Louisiana State University, Baton Rouge 70803, USA.
Chen
Z Y
ZY
Mouton
T M
TM
Moroney
J V
JV
D000818:Animals; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D002245:Carbon Dioxide