title: [Detection of minimal residual leukemia using polymerase chain reaction method].
abstract: Until recently, it was impossible to identify leukemia cells making up less than 1% of a bone marrow sample, which is designated as minimal residual disease (MRD). Owing to the development of the polymerase chain reaction (PCR) MRD can be detected at the 10(-5) level by amplifying the leukemia-specific DNA rearrangement (BCR/ABL, PML/RARA etc.) or clone-specific DNA sequences (IgH chain CDR-III etc.). Here, our studies on MRD of acute lymphoblastic leukemia and acute promyelocytic leukemia are presented, and their technical problems and clinical significance are discussed.
authors: Nagoya University Branch Hospital.
authors: T
authors: T
authors: Naoe
mesh_terms: D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis*; D018365:Neoplasm, Residual / Q000175:diagnosis; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm