Immobilization of xanthine oxidase on a polyaniline silicone support.
A polyaniline silicone support to immobilize xanthine oxidase is proposed as a reactor coil to monitor the action of xanthine oxidase on hypoxanthine, xanthine and 6-mercaptopurine. A purified xanthine oxidase immobilized on this support lost 80% of the initial activity after 12 min of use. Co-immobilization of superoxide dismutase and catalase increased the stability of immobilized xanthine oxidase so that the derivative maintained 79% of its initial activity after 4.6 h of continuous use in which 1.5 mumol purine bases were converted by the immobilized enzyme system. There is no evidence of either polyaniline or protein leaching from the coil during 3 h of continuous use. When solutions (10 ml) of hypoxanthine, xanthine and 6-mercaptopurine were circulated individually through the xanthine oxidase-superoxide dismutase-catalase-polyaniline coil (1 mm internal diameter and 3 m in length, 3 ml internal volume) activities of 8.12, 11.17 and 1.09 nmol min-1 coil-1, respectively, were obtained. The advantages of the reactor configuration and the redox properties of the polymer, particularly with respect to immobilized oxidoreductases, make this methodology attractive for similar enzyme systems. This immobilized enzyme system using polyaniline-silicone as support converted 6-mercaptopurine to 6-thiouric acid with equal efficiency as resins based on polyacrylamide and polyamide 11.
Nadruz
W
W
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE Brasil.
Marques
E T
ET
Azevedo
W M
WM
Lima-Filho
J L
JL
Carvalho
L B
LB
D004800:Enzymes, Immobilized / Q000378:metabolism; D066298:In Vitro Techniques; D012828:Silicones; D014969:Xanthine Oxidase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D012828:Silicones; D014969:Xanthine Oxidase