In vitro cytotoxicity and immunomodulating property of Rhaphidophora korthalsii.
An in vitro cytotoxic screening of extracts of Rhaphidophora korthalsii indicated cytotoxicity in the ether fraction. ED50 values of the extract against P388, Molt 4, KB and SW 620 were 12, 14, 8 and 13 micrograms/ml, respectively. The extract was relatively more toxic on P388 and Molt 4 cell lines at concentrations of 50 micrograms/ml and 100 micrograms/ml. Screening with mouse splenocytes showed that the hot water extract had splenocytes stimulating activity.
Wong
K T
KT
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore.
Tan
B K
BK
D000230:Adenocarcinoma; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms; D004396:Coloring Agents / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007938:Leukemia; D007941:Leukemia P388; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009303:Nasopharyngeal Neoplasms; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D037121:Plant Lectins; D010946:Plants, Medicinal; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014867:Water / Q000737:chemistry
D000276:Adjuvants, Immunologic; D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D010936:Plant Extracts; D037121:Plant Lectins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; D003208:Concanavalin A; C022616:thiazolyl blue