Immunohistochemical detection of virulence-associated antigens of Rhodococcus equi in pulmonary lesions of foals.
Rhodococcus equi was isolated from the lungs of six foals with bronchopneumonia. All isolates expressed 15-17-kd antigens by immunoblot analysis and contained a virulence-associated plasmid of 85 or 90 kb. Immunohistochemically, R. equi from all pulmonary lesions showed the expression of 15-17-kd antigens mainly in the phagocytic cells. The specific monoclonal antibody to 15-17-kd antigens of R. equi (MAb 10G5) may be an aid in the diagnosis of R. equi-induced pneumonia.
Madarame
H
H
Department of Laboratory Animal Science, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan.
Takai
S
S
Morisawa
N
N
Fujii
M
M
Hidaka
D
D
Tsubaki
S
S
Hasegawa
Y
Y
D000193:Actinomycetales Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001996:Bronchopneumonia / Q000209:etiology / Q000662:veterinary / Q000821:virology; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D006736:Horses; D007150:Immunohistochemistry / Q000379:methods; D008168:Lung / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D008297:Male; D016929:Rhodococcus equi / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial