title: Induction of apoptosis and secondary necrosis in rat dorsal root ganglion cell cultures by oxidized low density lipoprotein.
abstract: Neural cell degeneration underlies central and peripheral nervous system disorders. In this study we examined the influence of oxidized low density lipoprotein (Ox-LDL) on rat dorsal root ganglion (DRG) cells in culture. Methods used were cell morphology, lactate dehydrogenase (LDH) release, the TUNEL-reaction and DNA fragmentation. Exposure of DRG cells to Ox-LDL for 24 h led to elevation of LDH in the culture medium; short term exposure (4 h) induced apoptosis, evidenced by DNA fragmentation and a positive TUNEL-reaction. DRG cells modified LDL in the presence of Cu2+ to mildly oxidized and to a small extent to fully oxidized forms; these in situ-generated LDL oxidation products were strongly toxic. These results suggest that Ox-LDL is a neurotoxin; it initiates apoptotic cell injury which progresses to necrosis and cell death.
authors: Institute of Clinical Biochemistry, University of Bonn, Germany.
authors: A
authors: A
authors: Papassotiropoulos
authors: M
authors: M
authors: Ludwig
authors: W
authors: W
authors: Naib-Majani
authors: G S
authors: GS
authors: Rao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D004247:DNA / Q000032:analysis; D005727:Ganglia, Spinal / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009336:Necrosis; D009410:Nerve Degeneration; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D003300:Copper; D004247:DNA; D007770:L-Lactate Dehydrogenase; D019327:Copper Sulfate