Mutation of two conserved arginine residues in the glucose transporter GLUT4 supresses transport activity, but not glucose-inhibitable binding of inhibitory ligands.
Two arginine residues (RR333/334) in the conserved GRR motif located in the endofacial loop between helix 8 and 9 of the glucose transporter GLUT4 were substituted for leucine and alanine, respectively. Reconstituted glucose transport activity of the construct (GLUT4-RR333/4LA) expressed in COS-7 or LM(TK-) cells was less than 10% of that of the wild-type GLUT4. In contrast, binding of the inhibitory ligand cytochalasin B and glucose-inhibitable photolabeling with IAPS-forskolin were not significantly affected. Exchange of a histidine residue (H337Q) previously believed to be involved in the binding of inhibitory ligands failed to affect any of the investigated parameters. These data suggest that positive charges in the GRR motif at the cytoplasmic surface of the transporter participate in the conformational changes of the carrier protein during the process of facilitated diffusion.
Wandel
S
S
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat der RWTH Aachen, Germany.
Schurmann
A
A
Becker
W
W
Summers
S A
SA
Shanahan
M F
MF
Joost
H G
HG
D000818:Animals; D001120:Arginine; D001692:Biological Transport; D002478:Cells, Cultured; D005576:Colforsin / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000502:physiology*; D009124:Muscle Proteins; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014162:Transfection
D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D005576:Colforsin; D003571:Cytochalasin B; D001120:Arginine