title: Semi-permissive replication and functional aspects of the immune response in a cotton rat model of human parainfluenza virus type 3 infection.
abstract: A cotton rat (Sigmodon fulviventer) model of human parainfluenza virus type 3 (HPIV-3) infection was used to study patterns of HPIV-3 replication in naive and immune hosts. Growth curves revealed that nasal and pulmonary tissues of naive animals were semi-permissive for virus replication, with amounts of progeny virus proportional to inoculating doses. In naive animals there was a total eclipse in nasal tissues beginning 4 h after inoculation. By contrast, there was only partial eclipse of virus in pulmonary tissues, most pronounced at 1 h after inoculation. Immune animals demonstrated a delayed eclipse in pulmonary tissues upon rechallenge. Infection with very low doses of HPIV-3 induced complete protection against high-dose challenge in the absence of systemic neutralizing antibody, suggesting a significant role for other systemic or local immune effectors.
authors: Department of Pediatrics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA. Ottolini@USUHSB.USUHS.MIL
authors: M G
authors: MG
authors: Ottolini
authors: D D
authors: DD
authors: Porter
authors: V G
authors: VG
authors: Hemming
authors: S A
authors: SA
authors: Hensen
authors: I R
authors: IR
authors: Sami
authors: G A
authors: GA
authors: Prince
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D008253:Macaca mulatta; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development / Q000276:immunology* / Q000502:physiology; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000821:virology; D016205:Sigmodontinae; D013997:Time Factors; D014779:Virus Replication