title: Yeast infection of sperm, oocytes and embryos after intravaginal culture for embryo transfer.
abstract: OBJECTIVE
  To report a case of an embryo culture infected with Candida albicans after intravaginal culture.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Private infertility practice and university medical center.
  
  
  METHODS
  A couple with tubal and male factor infertility.
  
  
  METHODS
  Superovulation, oocyte recovery, Percoll sperm preparation, and intravaginal culture of sperm and oocytes in a tissue culture tube.
  
  
  METHODS
  Yeast infection of sperm, oocyte, and embryo culture.
  
  
  RESULTS
  Candida albicans infection occurred in the sperm, oocyte, and embryo culture when cultured in a sealed tube in the vagina. Candida albicans also was found in the prepared sperm suspension culture in a separate tube kept in a 37 degrees C incubator.
  
  
  CONCLUSIONS
  Infection of the embryo culture with C. albicans probably occurred when contaminated sperm was added at the time of insemination. Sperm preparation by the Percoll gradient centrifugation failed to eliminate C. albicans in the semen.
authors: Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences.
authors: M M
authors: MM
authors: Mahadevan
authors: F
authors: F
authors: Batres
authors: M M
authors: MM
authors: Miller
authors: D M
authors: DM
authors: Moutos
mesh_terms: D000328:Adult; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis / Q000209:etiology*; D002499:Centrifugation, Density Gradient / Q000379:methods; D004624:Embryo Transfer / Q000009:adverse effects* / Q000379:methods; D004622:Embryo, Mammalian / Q000382:microbiology*; D005260:Female; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods; D008297:Male; D009865:Oocytes / Q000382:microbiology*; D011247:Pregnancy; D013094:Spermatozoa / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology