title: Expression of carbonic anhydrase II mRNA and protein in oligodendrocytes during toxic demyelination in the young adult mouse.
abstract: The aim of this study was to identify events that might take place in oligodendrocytes early in the process of demyelination, i.e., before the occurrence of massive loss of myelin. It was considered important to focus on demyelination and remyelination in young adults, in whose brains there would be relatively few juvenile glial precursor cells. CAII mRNA and protein were used to monitor changes in oligodendrocytes during cuprizone intoxication in the mice. After four or eight weeks of cuprizone feeding CAII message became less plentiful in oligodendrocyte processes. Two days after removal of cuprizone CAII message had appeared in those cell processes. Four or eight weeks after beginning cuprizone feeding CAII protein had decreased approximately 25% in forebrain homogenates. The loss of CAII protein was reversible after four weeks on cuprizone, but not after eight weeks. After four weeks of cuprizone feeding the numbers of CAII mRNA-positive oligodendrocytes had decreased by approximately 50%, and after eight weeks, by approximately 80%. By 12 weeks, however, the number of oligodendrocytes expressing CAII mRNA had spontaneously returned to normal levels. Before eight weeks of cuprizone feeding, loss of myelinated tracts in the corpus striatum was reversible. Demyelination appeared to become irreversible after nine weeks of intoxication, although expression of CAII mRNA remained reversible. The results suggest that in the brain of the young adult, oligodendrocytes expressing message for CAII can be generated spontaneously shortly before demyelination becomes irreversible, and can survive and continue to express CAII mRNA but not CAII protein.
authors: Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors: F A
authors: FA
authors: Tansey
authors: H
authors: H
authors: Zhang
authors: W
authors: W
authors: Cammer
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D003471:Cuprizone / Q000633:toxicity*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D009186:Myelin Sheath / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D016548:Prosencephalon / Q000187:drug effects / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis*; D013997:Time Factors
chemical_list: D000906:Antibodies; D012333:RNA, Messenger; D003471:Cuprizone; D002256:Carbonic Anhydrases