Gas chromatographic determination of parathion and paraoxon in fish plasma and tissues.
A sensitive capillary gas chromatographic (GC) method for the simultaneous determination of the organophosphate insecticide, parathion, and its active metabolite, paraoxon, in biological samples was developed. This method involved a simple liquid-liquid extraction of parathion and paraoxon from water, plasma, or tissues and capillary GC determination using electron-capture detection and splitless injection; malathion was used as the internal standard. A gradient oven temperature program was used; the injection port and detector temperatures were 200 and 300 degrees C, respectively. These techniques allowed quantitative determination of parathion and paraoxon at 9-210-ng/ml. concentrations;recoveries ranged from 79.4 to 110.3% for tissues and from 91.9 to 100.0% for plasma and water. The within-day and between-day coefficients of variation were less than 8.0%. The method was used to characterize the pharmacokinetics of parathion and paraoxon and the tissue distribution of paraoxon in rainbow trout.
Abbas
R
R
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Ohio State University, Columbus 43210, USA.
Hayton
W L
WL
D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas; D007306:Insecticides / Q000032:analysis* / Q000097:blood; D007668:Kidney / Q000737:chemistry; D008294:Malathion / Q000097:blood / Q000378:metabolism; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D017686:Oncorhynchus mykiss; D010261:Paraoxon / Q000032:analysis* / Q000097:blood; D010278:Parathion / Q000032:analysis* / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D014018:Tissue Distribution
D007306:Insecticides; D010278:Parathion; D010261:Paraoxon; D008294:Malathion