Antigenic determinants at the carboxy terminus of chicken egg white riboflavin carrier protein (RCP): epitope mapping and antibody-mediated pregnancy curtailment in rodents.
The epitopic core sequences recognized by three monoclonal antibodies raised to chicken riboflavin carrier protein (RCP) were mapped to the C-terminal tail-end of the protein using the pepscan method. A 21-residue synthetic peptide corresponding to residues 200-219 of the protein and comprising the regions corresponding to the antibodies was synthesized. Administration of polyclonal antibodies specific to this peptide led to termination of early pregnancy in mice. Also, active immunization of rats with the peptide-purified protein derivative conjugate inhibited establishment of pregnancy. These results demonstrate the functional importance of the C-terminal 200-219 region of chicken RCP.
Koshy
B T
BT
Center for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore, India.
Karande
A A
AA
Adiga
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology*; D002645:Chickens; D003269:Contraception, Immunologic; D018604:Epitope Mapping; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005260:Female; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D002352:Carrier Proteins; D000939:Epitopes; D026901:Membrane Transport Proteins; D010446:Peptide Fragments; C019079:riboflavin-binding protein