Measurement of a new anticonvulsant, (S)-3-(aminomethyl)-5-methylhexanoic acid, in plasma and milk by high-performance liquid chromatography.
A specific and sensitive isocratic method for the measurement of a new anticonvulsant, (S)-3-(aminomethyl)-5-methylhexanoic acid, in rat plasma and milk is described. Following deproteinization, the compound and internal standard [1-(aminomethyl)cycloheptaneacetic acid] were derivatized utilizing 2,4,6-trinitrobenzene sulfonic acid and extracted with cyclohexane. Analytes were resolved on a 5 microns Spherisorb ODSII column (250 mm x 4.6 mm) using a mobile phase of 57% acetonitrile in 0.1 M ammonium acetate, pH 4.0. Absorbance was monitored at 350 nm. Limit of quantitation was 1.00 microgram/ml for a 100-microliters aliquot of plasma or milk.
Windsor
B L
BL
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA.
Radulovic
L L
LL
D000818:Animals; D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002138:Calibration; D002208:Caproates / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003508:Cycloheptanes / Q000032:analysis; D005260:Female; D008892:Milk / Q000737:chemistry*; D000069583:Pregabalin; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D014302:Trinitrobenzenesulfonic Acid; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives
D000927:Anticonvulsants; D002208:Caproates; D003508:Cycloheptanes; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; C098836:1-(aminomethyl)cycloheptaneacetic acid; D014302:Trinitrobenzenesulfonic Acid