Simultaneous determination of isosorbide dinitrate and its mononitrate metabolites in human plasma by capillary gas chromatography with electron-capture detection.
A method for the simultaneous determination of isosorbide dinitrate (ISDN) and its mononitrate metabolites (2- and 5-ISMN) in human plasma by capillary gas chromatography with electron-capture detection was developed. Two internal standards were used: isomannide dinitrate (IMDN) for the determination of ISDN and isomannide mononitrate (IMMN) for the determinations of 2- and 5-ISMN. After addition of the internal standards, the compounds were isolated from plasma by solid-liquid extraction. They were determined by gas chromatography using an electron-capture detector. The reproducibility and accuracy of the method were found suitable in the range of concentrations 2.5-83 ng/ml for ISDN, 2.6-208 ng/ml for 2-ISMN and 2.3-1010 ng/ml for 5-ISMN. The limit of quantitation (LOQ) was about 2.5 ng/ml for each compound. The method was applied to clinical samples.
Pommier
F
F
Laboratoires Ciba-Geigy, Bioanalytics and Pharmacokinetics, Rueil Malmaison, France.
Gauducheau
N
N
Pineau
V
V
Sioufi
A
A
Godbillon
J
J
D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D006801:Humans; D007548:Isosorbide Dinitrate / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014665:Vasodilator Agents / Q000097:blood*
D014665:Vasodilator Agents; D007548:Isosorbide Dinitrate