Determination of ketamine and norketamine enantiomers in plasma by solid-phase extraction and high-performance liquid chromatography.
A high-performance liquid chromatographic method is described for determination of sub-anaesthetic concentrations of the enantiomers of ketamine and its metabolite norketamine in plasma. The samples are purified by reversed-phase solid-phase extraction. The enantiomers are separated on a Chiral AGP column with a mobile phase containing 16% methanol and a 10 mM phosphate buffer at pH 7.0, and measured by UV-detection at a wavelength of 220 nm. Linear calibration curves with correlation coefficients better than 0.995 have been obtained in the range 10-320 ng/ml. Minimum detectable concentrations were about 2 ng/ml.
Svensson
J O
JO
Karolinska Institute, Department of Medical Laboratory Sciences & Technology, Huddinge University Hospital, Sweden.
Gustafsson
L L
LL
D000778:Anesthetics, Dissociative / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007649:Ketamine / Q000031:analogs & derivatives* / Q000097:blood*; D000432:Methanol; D008832:Microchemistry; D013237:Stereoisomerism
D000778:Anesthetics, Dissociative; D007649:Ketamine; C033419:norketamine; D000432:Methanol