Detection of turkey rhinotracheitis virus from chickens with swollen head syndrome by reverse transcriptase-polymerase chain reaction (RT-PCR).
We developed a sensitive and specific method, reverse transcriptase-polymerase chain reaction (RT-PCR) method, for detection of turkey rhinotracheitis virus (TRTV). Two sets of primers were designed from F protein gene sequence of TRTV 3B strain. Sensitivity of detection by nested PCR with the primers corresponded to 0.4 TCID50. Applying this method to a field case of swollen head syndrome (SHS), TRTV could be detected directly from chicken trachea and turbinates. It was identified that this method was very useful to examine the relation of TRTV and SHS.
Mase
M
M
National Institute of Animal Health, Ibaraki, Japan.
Asahi
S
S
Imai
K
K
Nakamura
K
K
Yamaguchi
S
S
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D018111:Pneumovirus / Q000235:genetics / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D013577:Syndrome; D014132:Trachea / Q000821:virology; D014420:Turbinates / Q000821:virology; D014422:Turkeys; D014760:Viral Fusion Proteins / Q000235:genetics
D017931:DNA Primers; D006023:Glycoproteins; D014760:Viral Fusion Proteins; C103938:glycoprotein F, turkey rhinotracheitis virus