STAT-like DNA-binding activity in Spodoptera frugiperda cells.
Nuclear extracts from Spodoptera frugiperda (Sf9) cells were shown to contain a factor binding to DNA elements with gamma interferon activated site-like sequences. The DNA-binding activity was shown to be dependent on tyrosine phosphorylation. Hydrodynamic characterization of this entity revealed a Stokes radius of 8.4 nm and a sedimentation coefficient of 5.9 S. The molecular weight was calculated to 209,000. Estimated frictional (f/to) and axial (a/b) ratios indicated an elongated structure of this DNA-binding entity. This DNA-binding factor could represent a dimer of a Sf9 homolog to the mammalian signal transducers and activators of transcription.
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Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden.
Haldosén
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D053616:Janus Kinase 3; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D015345:Oligonucleotide Probes; D010766:Phosphorylation; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D016415:Sequence Alignment; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism*
D003470:Culture Media; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011505:Protein-Tyrosine Kinases; D053616:Janus Kinase 3; D017027:Protein Tyrosine Phosphatases