title: Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome.
abstract: OBJECTIVE
  To assess the potential involvement of cytokines and nitrites in the hyperpermeability characterizing the ovarian hyperstimulation syndrome (OHSS).
  
  
  METHODS
  A controlled clinical study comparing peritoneal fluid (PF) from patients with severe OHSS and from non-OHSS controls.
  
  
  METHODS
  Women hospitalized with severe OHSS in three tertiary medical centers.
  
  
  METHODS
  Twelve patients with severe OHSS necessitating paracentesis and 20 non-OHSS controls.
  
  
  METHODS
  The criteria for ultrasound-guided paracentesis were tense ascites, hydrothorax, hemoconcentration, or oliguria.
  
  
  METHODS
  Interleukin (IL) 1 beta IL-1 receptor agonist, IL-2, IL-6, IL-8, and tumor necrosis factor alpha (TNF alpha) levels in PF were assayed by ELISA; nitrites were measured by the "Griess" reaction. Estradiol and P were determined by RIA.
  
  
  RESULTS
  Ovarian hyperstimulation syndrome patients had significantly higher PF IL-6 (3,523 versus 30 pg/mL), TNF alpha (14 versus 4.2 pg/mL), and IL-8 (1,695 versus 900 pg/mL). In the serum, only IL-6 levels were significantly higher (375 versus 11 pg/mL). Conversely, nitrite levels were significantly lower in PF of OHSS patients (0.5 versus 34 nmol/mL). Interleukin 1 levels were higher and IL-1 receptor antagonist levels were lower in OHSS patients, suggesting potentially increased biologic potency of IL-1.
  
  
  CONCLUSIONS
  These findings suggest that these substances could be involved in mediating the capillary hyperpermeability characterizing this syndrome.
authors: Department of Obstetrics and Gynecology, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel. revel@.yam-suff.cc.huji.ac.il
authors: A
authors: A
authors: Revel
authors: V
authors: V
authors: Barak
authors: Y
authors: Y
authors: Lavy
authors: E
authors: E
authors: Anteby
authors: Y
authors: Y
authors: Abramov
authors: J J
authors: JJ
authors: Schenker
authors: A
authors: A
authors: Amit
authors: Z
authors: Z
authors: Finci-Yeheskel
authors: M
authors: M
authors: Mayer
authors: A
authors: A
authors: Simon
authors: N
authors: N
authors: Laufer
authors: A
authors: A
authors: Hurwitz
mesh_terms: D001202:Ascitic Fluid / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D016471:Ovarian Hyperstimulation Syndrome / Q000378:metabolism*
chemical_list: D016207:Cytokines; D007378:Interleukins; D009566:Nitrates; D009573:Nitrites