Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases.
Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of alpha 1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As the the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.
Bini
L
L
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy.
Magi
B
B
Marzocchi
B
B
Cellesi
C
C
Berti
B
B
Raggiaschi
R
R
Rossolini
A
A
Pallini
V
V
D000209:Acute-Phase Proteins / Q000032:analysis*; D001424:Bacterial Infections / Q000097:blood*; D002644:Chickenpox / Q000097:blood / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D006192:Haemophilus Infections / Q000097:blood / Q000378:metabolism; D006193:Haemophilus influenzae / Q000302:isolation & purification; D006801:Humans; D008457:Measles / Q000097:blood / Q000378:metabolism; D009107:Mumps / Q000097:blood / Q000378:metabolism; D012475:Salmonella / Q000302:isolation & purification; D012480:Salmonella Infections / Q000097:blood / Q000378:metabolism; D013290:Streptococcal Infections / Q000097:blood / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000302:isolation & purification; D014777:Virus Diseases / Q000097:blood*
D000209:Acute-Phase Proteins