Human serum immunoglobulins binding to synthetic fragments of human chorionic gonadotropin.
Human sera obtained from healthy individuals, patients with cancer diseases, infertile and pregnant women were investigated for the presence of immunoglobulins (Ig) binding to synthetic fragments corresponding to sequence of human chorionic gonadotropin (hCG). Four synthetic peptides corresponding to some regions of alpha hCG and beta hCG subunits, coupled to polystyrene beads and 16 peptides overlapping sequences of alpha- and beta hCG subunits, synthesized on polyethylene pins, were used. IgG and IgM bound to hCG-synthetic peptides were assayed using goat or mouse monoclonal antibodies anti-human IgG labeled with horseradish peroxidase. Most of the investigated sera contained IgG which bound to examined synthetic peptides, but obtained results were dependent on serum donors. Some relationships between results obtained with peptides synthesized on modified pins according to Geysen et al. method and corresponding peptides synthesized by Merrifield method and coupled to beads were noted.
Kochanowska
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Biochemical Laboratory, Polish Academy of Sciences, Wrocław, Poland.
Szewczuk
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D000328:Adult; D000368:Aged; D000818:Animals; D002675:Child, Preschool; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D005260:Female; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism*; D008297:Male; D051379:Mice; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism*; D011247:Pregnancy
D006063:Chorionic Gonadotropin; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments