Transfer of T-cell-mediated, antigen-specific delayed type hypersensitivity reactions to naive recipient inbred pigs.
Using inbred major histocompatibility complex-homozygous SLAb/b pigs, delayed-type hypersensitivity (DTH) reactions against either intradermal tuberculin (PPD) or topical 2,4-dinitro-1-fluorobenzene (DNFB) were transferred specifically by the intravenous injection of approximately 6 x 10(8) blood lymphocytes kg-1 bodyweight from donors sensitised, respectively, either with BCG or with DNFB into three-week-old piglets from an inbred litter. This antigen-specific, passively acquired sensitivity was revealed by three measures of DTH reactivity: first, macroscopic inflammation, which developed at the rate and intensity expected for actively acquired sensitivity to DNFB or PPD in older pigs; secondly, similarly enhanced local specific uptake of intravenously injected 51Cr-labelled normal lymphocytes (more than 35-fold for each); and, thirdly, histological evidence of markedly increased local infiltration of CD45+ lymphocytes and polymorphs, endothelial activation and the expression of adhesion molecules.
Binns
R M
RM
Immunology Department, Babraham Institute, Cambridge 001251.
Licence
S T
ST
Whyte
A
A
D000818:Animals; D004139:Dinitrofluorobenzene / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed; D016219:Immunotherapy, Adoptive; D007249:Inflammation; D017710:Lymphocyte Transfusion; D008297:Male; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
D014373:Tuberculin; D004139:Dinitrofluorobenzene