A sensitive human cell line based bioassay for megakaryocyte growth and development factor or thrombopoietin.
We have developed a simple, rapid, sensitive bioassay for megakaryocyte growth and development factor (MGDF) or thrombopoietin (TPO) based on its ability to stimulate the proliferation of the human megakaryoblastic cell line MO7e. The bioassay takes only 24 h, is reproducible and sensitive to less than 10 pg/ml of MGDF. It can be made specific for MGDF by including neutralizing antibodies specific for MGDF, and is suitable for detection of MGDF in both plasma and serum samples.
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Division of Immuniobiology, National Institute for Biological Standards and Control, Herts EN6.
Thorpe
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Mire-Sluis
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D000906:Antibodies; D001681:Biological Assay / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008533:Megakaryocytes; D009500:Neutralization Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013926:Thrombopoietin / Q000032:analysis* / Q000494:pharmacology*
D000906:Antibodies; D013926:Thrombopoietin