Reproduction in mice lacking a functional type 1 IL-1 receptor.
Growth factors and cytokines have been identified in having critical roles at mediating maternal-fetal interactions during pregnancy, with interleukin-1 being a recently implicated factor. Previous experiments indicated that repeated intraperitoneal injections of the Il-1 receptor antagonist (Il-1Ra), which inhibits binding of interleukin-1 (Il-1) to the type 1 Il-1 receptor (Il-1Rt1) blocks blastocyst implantation in superovulated mice. To gain a greater insight into the role of Il-1 receptor in implantation, we analyzed the reproduction of mice deficient for the Il-1Rt1. Our results show that mice lacking this receptor do not exhibit any profound alterations in their reproduction, apart from a slight reduction in mean litter size. Furthermore, repeated intraperitoneal injections of either IL-1Ra or the monoclonal antibody 35F5, which also blocks ligand binding to the Il-1Rt1, did not affect embryo implantation in either wild type and Il-1 receptor deficient mice.
Abbondanzo
S J
SJ
Roche Institute of Molecular Biology, Hoffmann-La Roche Inc., Nutley, New Jersey 07110, USA.
Cullinan
E B
EB
McIntyre
K
K
Labow
M A
MA
Stewart
C L
CL
D000818:Animals; D000911:Antibodies, Monoclonal; D010064:Embryo Implantation; D005260:Female; D006720:Homozygote; D008098:Litter Size; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics* / Q000502:physiology; D011247:Pregnancy; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D012098:Reproduction; D013997:Time Factors; D014599:Uterus / Q000473:pathology
D000911:Antibodies, Monoclonal; D017472:Receptors, Interleukin-1