Expression of apolipoprotein E inhibits aggregation of the C-terminal fragments of beta-amyloid precursor protein.
An important role of apolipoprotein E in the amyloidogenesis of Alzheimer's disease is suggested by an accumulation of apolipoprotein E in beta-amyloid plaques and a genetic association between Alzheimer's disease and one of the allelic variants (APOE4) of apolipoprotein E. Overexpression of a C-terminal region of beta-amyloid precursor protein brings about aggregation of the C-terminal fragments in COS cells. This COS cell culture system was used to study effects of apolipoprotein E on aggregation of the C-terminal fragments. When both apolipoprotein E and the C-terminal fragments were overexpressed in COS cells, Western blot analyses revealed significant inhibition of aggregation of the C-terminal fragments. No significant differences between apolipoprotein E3 and E4 in the inhibitory activities were found by this method. Apolipoprotein E may inhibit formation of amyloid fibrils.
Ohman
T
T
Department of Pathology, University of Washington, Seattle 98195, USA.
Dang
N
N
LeBoeuf
R C
RC
Furlong
C E
CE
Fukuchi
K
K
D000544:Alzheimer Disease / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000686:Amyloidosis; D000818:Animals; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E / Q000096:biosynthesis / Q000378:metabolism*; D015153:Blotting, Western; D019556:COS Cells / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism*; D014162:Transfection
D016564:Amyloid beta-Protein Precursor; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E; D018076:DNA, Complementary; D010446:Peptide Fragments