Fluorescent in situ hybridization on archival G-banded slides.
We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 x SSC, Tween 20.
el Rifai
W
W
Department of Medical Genetics, University of Helsinki, Finland.
Knuutila
S
S
D002871:Chromosome Banding; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics / Q000473:pathology; D011309:Preservation, Biological; D013048:Specimen Handling; D014178:Translocation, Genetic