The majority of autologous cytolytic T-lymphocyte clones derived from peripheral blood lymphocytes of a melanoma patient recognize an antigenic peptide derived from gene Pmel17/gp100.
Anti-melanoma cytolytic T-lymphocyte (CTL) clones were derived from peripheral blood lymphocytes of HLA-A2 melanoma patient LB265 after stimulation with the autologous tumor cell line LB265-MEL, which showed high expression of melanocyte-lineage specific genes. Of 55 CTL clones, 46 recognized HLA-A2-restricted antigens. These 46 CTL clones were studied for their ability to specifically release tumor necrosis factor in the presence of COS cells cotransfected with the HLA-A2 gene and the cDNA of either tyrosinase, Melan-A/MART1, Pmel17/gpl00, gp75/TRP1, or MSH receptor. Six CTL clones recognized the Melan-A/MART1 antigen, whereas the remaining 40 CTL clones recognized a Pmel17/gp100 antigen. These 40 anti-PmelI7/gpl00 CTL clones were all able to lyse T2 cells pulsed with the antigenic peptide YLEPGPVTA, as previously reported. The T-cell receptor beta chain hypervariable region was sequenced and found to be identical in the 15 CTL clones analyzed. Taken together, these data show a high frequency of Pmell7/gp100-specific T cells in autologous antitumor CTL clones derived from peripheral blood of a melanoma patient.
Zarour
H
H
The Ludwig Institute for Cancer Research, University of Louvain, Brussels, Belgium.
De Smet
C
C
Lehmann
F
F
Marchand
M
M
Lethé
B
B
Romero
P
P
Boon
T
T
Renauld
J C
JC
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D002999:Clone Cells; D005260:Female; D005796:Genes; D015789:HLA-A2 Antigen / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; D015345:Oligonucleotide Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta