Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells.
Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.
Müller
B K
BK
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany.
Kabos
P
P
Belhage
B
B
Neumann
T
T
Kater
S B
SB
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology; D016257:Fura-2 / Q000494:pharmacology; D006706:Homeostasis; D007476:Ionophores / Q000494:pharmacology; D010320:Parvalbumins / Q000235:genetics / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D018243:Teratocarcinoma; D014162:Transfection; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D007476:Ionophores; D010320:Parvalbumins; D000001:Calcimycin; D011189:Potassium Chloride; D002118:Calcium; D016257:Fura-2