[Informative capacity of 8 serological tests in the diagnosis of human brucellosis].
Assessment of the informative value of 8 immunological tests: sero-agglutination (Wright and Rose Bengale), indirect immunosorbent assay, counter immuno electrophoresis, ELISA IgG, IgM and IgA, and particle counting immunoassay (PACIA) has been performed among the results of serum of 209 patients. The patients were divided in four groups: 71 who already had brucellosis, 18 Yersinia infections, 12 Tularemia and 108 free of desease. The informative capacities of a positive result of counter immuno electrophoresis (Protein antigen Brucellin-INRA Tours-Nouzilly) is higher than others reactions and can be proposed as a confirmatory test of brucellosis. Among others techniques, 4 were found to be more sensitive: Elisa IgA (se = 97.6) and IgG (se = 90.1), IFI (se = 91.5) and Rose Bengale (se = 85.9) and can be proposed as screening test for medical diagnosis or epidemiological survey. Many cross-reactions were observed specially with Yersinia enterocolitica even with new serological methods.
Laudat
P
P
Laboratoire de Bactériologie, CHU de TOURS, France.
Van Der Mee-Marquet
N
N
Loulergue
J
J
Audurier
A
A
D000372:Agglutination Tests; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques; D014406:Tularemia / Q000175:diagnosis* / Q000276:immunology; D015009:Yersinia Infections / Q000175:diagnosis* / Q000276:immunology
Capacité informative de huit tests sérologiques dans le diagnostic de la brucellose humaine.