Deoxycytidyl transferase activity of yeast REV1 protein.
Mutagenesis induced by DNA damage in Saccharomyces cerevisiae requires the products of the REV1, REV3 and REV7 genes. The Rev3 and Rev7 proteins are subunits of DNA polymerase-zeta (Pol-zeta), an enzyme whose sole function appears to be translesion synthesis. Rev1 protein has weak homology with UmuC protein which facilitates translesion synthesis in Escherichia coli by an unknown mechanism. We show here that Rev1 protein has a deoxycytidyl transferase activity which transfers a dCMP residue from dCTP to the 3' end of a DNA primer in a template-dependent reaction. Efficient transfer occurred opposite a template abasic site, but approximately 20% transfer also occurred opposite a template guanine and approximately 10% opposite adenine or uracil; < or = 1% was seen opposite thymine or cytosine. Insertion of cytosine opposite an abasic site produced a terminus that was extended efficiently by Pol-zeta, but not by yeast Pol-alpha.
Nelson
J R
JR
Department of Biophysics, University of Rochester, New York 14642-8408, USA.
Lawrence
C W
CW
Hinkle
D C
DC
D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000378:metabolism; D004926:Escherichia coli; D005656:Fungal Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D000099270:Y-Family DNA Polymerases
D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D003845:Deoxycytosine Nucleotides; D005656:Fungal Proteins; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C024107:2'-deoxycytidine 5'-triphosphate; C100951:REV1 protein, S cerevisiae; D000099270:Y-Family DNA Polymerases