Three genes for mitochondrial proteins suppress null-mutations in both Afg3 and Rca1 when over-expressed.
The AFG3 gene of Saccharomyces cerevisiae encodes a mitochondrial inner membrane protein with ATP-dependent protease activity. To gain more insight into the function of this protein, multi-copy suppressors of an afg3-null mutation were isolated. Three genes were found that restored partial growth on non-fermentable carbon sources, all of which affect the biogenesis of respiratory competent mitochondria: PIM1(LON) encodes a matrix-localized ATP-dependent protease involved in the turnover of matrix proteins; OXA1(PET1402) encodes a putative mitochondrial inner membrane protein involved in the biogenesis of the respiratory chain; and MBA1 encodes a mitochondrial protein required for optimal respiratory growth. All three genes also suppressed a null mutation in a related gene, RCA1, as well as in the combination of afg3- and rca1-null.
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Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Nooy
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Guélin
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Grivell
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LA
D049069:ATP-Dependent Proteases; D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D015698:Genomic Library; D005838:Genotype; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008666:Metalloendopeptidases; D008928:Mitochondria / Q000235:genetics; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics; D013489:Suppression, Genetic
D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C090858:OXA1 protein; D029701:Saccharomyces cerevisiae Proteins; D003576:Electron Transport Complex IV; D049069:ATP-Dependent Proteases; C085155:PIM1 protein, S cerevisiae; D012697:Serine Endopeptidases; D008666:Metalloendopeptidases; D000251:Adenosine Triphosphatases; C102166:YTA12 protein, S cerevisiae; C090045:AFG3 protein, S cerevisiae