Putrescine transport in differentiated Caco-2 cells.
OBJECTIVE
To evaluate the putative importance of digestive luminal polyamines, we investigated the role of oxidation in putrescine uptake and transport in differentiated Caco-2 cells grown in monolayer.


RESULTS
Our results confirm a putrescine uptake system located both at the apical and basolateral cell membrane. Putrescine is efficiently transported and excreted in the apical-to-basolateral direction.


CONCLUSIONS
These results suggest (i) a diamine oxidase controlled catabolic oxidative excreting pathway and (ii) a paracellular route for putrescine transport. This catabolic route seems to proceed by converting putrescine into gamma-aminobutyraldehyde which is then rapidly excreted from the cell at the basolateral membrane since no gamma-aminobutryraldehyde was detected in the cell. This putative basolateral excreting mechanism is inhibited in the presence of the diamine oxidase inhibitor, aminoguanidine.
Barlier
A M
AM
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, Paris.
Benamouzig
R
R
Tomé
D
D
D001693:Biological Transport, Active; D018938:Caco-2 Cells / Q000187:drug effects*; D003538:Cystamine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008935:Mitoguazone / Q000494:pharmacology; D011700:Putrescine / Q000493:pharmacokinetics / Q000494:pharmacology*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D006146:Guanidines; D013096:Spermine; D008935:Mitoguazone; D003538:Cystamine; C004479:pimagedine; D013095:Spermidine; D011700:Putrescine