title: [Autoradiographic studies of oxalate distribution in rat kidney].
abstract: BACKGROUND
  We performed macro and micro-autoradiographic studies using 14C-oxalate in normal and hyperoxaluric rats in order to elucidate intrarenal distribution of oxalate and to determine where calcium oxalate crystal can be adhere in the kidney.
  
  
  METHODS
  Macro and micro-autoradiographic studies of the kidney in normal rats were carried out at intervals of 15, 30 and 90 min after intravenous administration of 14C-oxalate (37 MBq/kg BW). Hyperoxaluria was induced by vitamin B6 deficient diet. The procedure of macro and micro autoradiography is the same as that in normal rat.
  
  
  RESULTS
  In normal rats, macroautoradiogram showed that the radioactivity of 14C-oxalate exists in the whole kidney, and microautoradiogram identified this radioactivity mainly in the extraluminal space of renal tubules at 15 min. Macro and micro-autoradiograms taken 90 min after the injection showed practically no radioactivity of 14C-oxalate in the cortex or the medulla, but it was located in the extraluminal space of the papilla. In hyperoxaluric rats, macroautoradiogram showed some spotty accumulations of 14C-oxalate in the inner medulla and papilla. Microautoradiogram revealed that these accumulations are mainly seen in the extraluminal space.
  
  
  CONCLUSIONS
  These results indicate that in normal rats the injected 14C-oxalate remains in the renal papilla, especially in the extraluminal space, when nearly all oxalate was excreted by urine, and in hyperoxaluric rats 14C-oxalate exists there as calcium oxalate crystal or microlith.
authors: Department of Chemistry, Osaka City University Medical School.
authors: S
authors: S
authors: Kamikawa
authors: T
authors: T
authors: Sugimoto
authors: T
authors: T
authors: Kanazawa
authors: K
authors: K
authors: Ishii
authors: Y
authors: Y
authors: Yoneda
authors: H
authors: H
authors: Iimori
authors: K
authors: K
authors: Yamamoto
authors: T
authors: T
authors: Kishimoto
authors: Y
authors: Y
authors: Funae
mesh_terms: D000818:Animals; D001345:Autoradiography; D006959:Hyperoxaluria / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010070:Oxalates / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D010070:Oxalates