Computer aided data acquisition and analysis of acetlycholinesterase velocity sedimentation profiles.
Acetylcholinesterase (AChE) is an enzyme that appears in several molecular forms. Individual AChE molecular forms can be isolated and identified on the basis of their velocity sedimentation profiles. We have improved an existing method for analyzing AChE sedimentation profiles with a computer aided data acquisition and analysis system. The software facilitates measurements of AChE activity of individual molecular forms and comparison among AChE velocity sedimentation profiles. In practice, our program increased precision and reduced the time of AChE velocity sedimentation profile analysis. The software can also be applied to velocity sedimentation analysis of other proteins. We plan to upgrade the program by making it compatible with Microsoft Windows.
Ribaric
S
S
Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Slovenia.
Peterec
D
D
Sketelj
J
J
D000110:Acetylcholinesterase / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods* / Q000706:statistics & numerical data; D003196:Computer Graphics; D003627:Data Interpretation, Statistical; D008297:Male; D009132:Muscles / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D013053:Spectrophotometry
D000110:Acetylcholinesterase