Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest.
OBJECTIVE
To see whether testicular sperm extraction could be used to perform intracytoplasmic sperm injection (ICSI) for men with nonobstructive azoospermia caused by maturation arrest.


METHODS
Uncontrolled prospective trial of an attempt to find occasional elongated spermatids or spermatozoa in testes of azoospermic patients with maturation arrest and to use these haploid cells for ICSI.


METHODS
European university-based center for reproductive medicine and private American community hospital.


METHODS
Thirty-eight azoospermic males without obstruction and with biopsy-documented maturation arrest, seven of whom elected, with their wives, to undergo scrotal exploration and testicular sperm extraction with ICSI in an attempt to become pregnant.


METHODS
Histologic evaluation of spermatid development in 38 patients with azoospermic maturation arrest. Testicular sperm extraction with ICSI in seven random volunteers from this group.


METHODS
Presence or absence of mature spermatids in the testis biopsy specimen of patients with azoospermic maturation arrest. Fertilization, cleavage, and pregnancy after testicular sperm extraction and ICSI in patients with azoospermic maturation arrest.


RESULTS
All seven patients with azoospermic maturation arrest had occasional sperm found with testicular sperm extraction. Five had sufficient numbers (between 6 and 30) for ICSI, and those five had ETs. In four, the partners became pregnant. In all 38 patients examined, the maturation defect was in meiosis rather than in spermiogenesis.


CONCLUSIONS
Nonobstructive azoospermia caused by maturation arrest may be treated with testicular sperm extraction with ICSI apparently as successfully as Sertoli cell only.
Silber
S J
SJ
St. Luke's Hospital, St. Louis, Missouri 63017, USA.
van Steirteghem
A
A
Nagy
Z
Z
Liu
J
J
Tournaye
H
H
Devroey
P
P
D016922:Cellular Senescence; D003593:Cytoplasm; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007267:Injections; D008297:Male; D008846:Micromanipulation; D009845:Oligospermia / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D009865:Oocytes; D011247:Pregnancy; D018873:Pregnancy Rate; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000473:pathology