Mechanisms of simian gamma delta T cell cytotoxicity against tumor and immunodeficiency virus-infected cells.
The mechanisms of cytotoxic killing of various tumor cell lines and immunodeficiency virus-infected T cell lines by simian gamma delta T cells were examined. The lysis of the majority of the target cell lines by gamma delta effectors was calcium-dependent, indicating that cytotoxicity is mediated by the perforin/granzyme pathway rather than the Fas-FasL pathway, with the exception of Jurkat cells. The gamma delta T cells were able to suppress SIV replication as measured by the p27 ELISA and the suppression was contact-dependent. We further determined that the target cells were induced to undergo apoptosis by the gamma delta T cell effectors. These results contribute to our understanding of the function of simian gamma delta T cells and their similarities to human gamma delta T cells, and extend our knowledge on the cytotoxic mechanisms employed by gamma delta T cells in general.
Gan
Y H
YH
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison 53706, USA.
Malkovsky
M
M
D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology; D017209:Apoptosis; D002118:Calcium / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D008253:Macaca mulatta; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D018941:Antigens, CD19; D016692:Receptors, Antigen, T-Cell, gamma-delta; D002118:Calcium