title: Amino acid sequence of the minor isomorph of the crustacean hyperglycemic hormone (CHH-II) of the Mexican crayfish Procambarus bouvieri (Ortmann): presence of a D-amino acid.
abstract: The primary structure of the neurohormone crustacean hyperglycemic hormone (CHH-II) was determined by means of enzymatic digestions, manual Edman degradation, and mass spectrometry. CHH-II is a 72 residue peptide (molecular mass 8388 Da), with six cysteines forming three disulfide bridges that connect residues 7-43, 23-39, and 26-52. The peptide has blocked N- and C-termini, and lacks tryptophan, histidine, and methionine. The CHH-I and CHH-II of Procambarus bouvieri have identical sequences and elicit levels of hyperglycemia that are not distinguishable. The difference between the two isomorphs consists in a posttranslational modification of a L-Phe in CHH-I to a D-Phe in CHH-II at the third position from the N-terminus.
authors: Department of Biochemistry, Instituto Nacional de la Nutrición S. Zubirán, Tlalpan, Mexico City, Mexico.
authors: M B
authors: MB
authors: Aguilar
authors: D
authors: D
authors: Soyez
authors: R
authors: R
authors: Falchetto
authors: D
authors: D
authors: Arnott
authors: J
authors: J
authors: Shabanowitz
authors: D F
authors: DF
authors: Hunt
authors: A
authors: A
authors: Huberman
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D060829:Arthropod Proteins; D003400:Astacoidea / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C015947:hyperglycemic hormone, crustacean; D003545:Cysteine