title: A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.
abstract: An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.
authors: Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. sorokine@biotec.jouy.inra.fr
authors: A
authors: A
authors: Sorokin
authors: A
authors: A
authors: Lapidus
authors: V
authors: V
authors: Capuano
authors: N
authors: N
authors: Galleron
authors: P
authors: P
authors: Pujic
authors: S D
authors: SD
authors: Ehrlich
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast / Q000378:metabolism; D002876:Chromosomes, Bacterial; D004265:DNA Helicases; D004269:DNA, Bacterial; D053488:DnaB Helicases; D016521:Electrophoresis, Gel, Pulsed-Field; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012407:rRNA Operon
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004265:DNA Helicases; D053488:DnaB Helicases