title: Determination of aglycones of ginsenosides in ginseng preparations sold in Sweden and in urine samples from Swedish athletes consuming ginseng.
abstract: Recently developed gas chromatographic and gas chromatographic-mass spectrometric methods were used to characterize 17 different commercial ginseng preparations sold in Sweden. The contents of total ginsenosides per capsule or per tablet varied from 2.1 to 13.3 mg. Unlike the other preparations, a red ginseng and three liquid ginseng preparations (after releasing the sugar moieties from ginsenosides) were shown also to contain significant amounts of 20-epimers of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol as well as their corresponding 24,25-hydrated compounds. In addition to the genuine and artificial sapogenins mentioned above, two epimeric pairs of prosapogenines (ginsenoside Rg3 and 20(S)-Rg3, ginsenoside Rh1 and 20(R)-Rh1) were also found in the liquid formulations. These results suggest that hydrolysis, epimerization and hydration in the side-chain of the aglycone moiety of ginsenosides may occur in the liquid formulations under weak acidic conditions (pH 3.0-3.5 with 9-10% of alcohol at room temperature). The new method was also used to determine the aglycones of ginsenosides in urine samples from Swedish athletes stating that they had consumed ginseng preparations within 10 days before urine collection. Out of the 65 samples analysed, 60 were found to contain 20(S)-protopanaxatriol. The concentrations of 20(S)-protopanaxatriol ginsenosides varied from 2 to 35 ng ml-1 urine. This is the first demonstration of uptake of ginsenosides in humans after oral administration of ginseng preparations.
authors: Department of Medical Laboratory Sciences and Technology, Huddinge University Hospital, Stockholm, Sweden.
authors: J F
authors: JF
authors: Cui
authors: M
authors: M
authors: Garle
authors: I
authors: I
authors: Björkhem
authors: P
authors: P
authors: Eneroth
mesh_terms: D036145:Ginsenosides; D006027:Glycosides / Q000737:chemistry* / Q000652:urine; D006801:Humans; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D012502:Sapogenins / Q000737:chemistry / Q000652:urine*; D012503:Saponins / Q000737:chemistry* / Q000652:urine; D013177:Sports; D013548:Sweden; D014315:Triterpenes
chemical_list: D036145:Ginsenosides; D006027:Glycosides; D012502:Sapogenins; D012503:Saponins; D014315:Triterpenes; C081552:protopanaxatriol