A novel MADS-box gene of potato (Solanum tuberosum L.) expressed during the early stages of tuberization.
A potato MADS-box gene cDNA (POTM1-1) from an early tuber cDNA library has been isolated and characterized. The deduced amino acid sequence of POTM1-1 cDNA encodes 250 amino acids of a putative transcription factor containing a MADS-box domain and a K-box domain. These conserved domains share high homologies to those of flower-specific homeotic proteins, TM4 of tomato and AP1 of Arabidopsis, indicating that POTM1-1 gene is a homologue of the AP1 gene family. The levels of POTM1-1 transcripts were high in axillary buds, underground stolen tips, and newly formed tubers, but relatively low in mature tubers. During axillary bud development in a model petiole-leaf cutting system, the levels of POTM1-1 transcripts were abundant in actively growing shoots and during the early stages of microtuber development. It is possible that POTM1-1 functions as a transcription factor that regulates plant developmental processes in a number of tissue types.
Kang
S G
SG
Department of Horticulture, Iowa State University, Ames 50011, USA.
Hannapel
D J
DJ
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D026161:MADS Domain Proteins; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014157:Transcription Factors