The role of recombinant human fusion protein IL-6/IL-2 (CH925) in hepadna virus infection treatment.
The effects of CH925, a novel immune modulator, on hepadna virus infection was evaluated. Day-old ducklings were inoculated intravenously with LJ-76 DHBV containing serum. Infected ducklings were then treated with the CH925 and the mixture of IL-2 and IL-6 intravenously and the control ducklings received equivalent normal saline (NS). Blood and liver samples were taken and assayed for DHBV DNA and/or DHBsAg. At the completion of the experiment there was a inhibition of viremia with the CH925 and IL-2 + IL-6. Serum DHBV DNA was detected in 6 of 10 ducks in 100,000 unit/kg dosage group, 7 of 10 ducks in 50,000 unit/kg dosage group and 6 of 10 ducks in IL-2 + IL-6 dosage group, compared with 9 of 10 NS control, and it showed a similar result in circulating DHBsAg. When samples of liver DNA were processed for hybridization, a little difference in the DHBV DNA replication was noted between ducks receiving CH925, IL-2 + IL-6 or NS placebo. It is suggested that CH925 might be a potential remedy in HBV infection treatment.
Mi
Z
Z
Institute of Microbiology and Epidemiology, Beijing.
Zhao
C
C
Zhang
X
X
Tang
P
P
Chen
H
H
D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000378:metabolism; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000378:metabolism*; D015611:Hepatitis B Virus, Duck / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
D000956:Antigens, Viral; D000998:Antiviral Agents; D004279:DNA, Viral; D007376:Interleukin-2; D015850:Interleukin-6; D011993:Recombinant Fusion Proteins; C089276:recombinant fusion protein IL-6 - IL-2