A murine model for the study of mycotic mastitis.
This study established a murine model for the study of mycotic mastitis. The mammary glands of BALB/c mice were inoculated on the fifth day of lactation with graded doses (10(4), 10(5) and 10(6) cells) of a pathogenic strain of Candida krusei isolated from bovine mastitis. The animals were killed 1, 2, 3, 4 or 5 days after inoculation. In the infected mammary glands, the pathological reaction consisted of primary infiltration with heterophils and mononuclear cells, focal necrosis, formation of microabscesses, epithelial hyperplasia and some fibrosis. The severity of the changes was dose-dependent and increased with time after infection. An increase in the plasma concentrations of complement factors C1, C3c, C4 and C5, factor B and alpha-2-macroglobulin suggested that an acute phase response and activation of the complement system had occurred as a result of the infection.
Guhad
F A
FA
Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, UK.
Jensen
H E
HE
Aalbaek
B
B
Rycroft
A
A
Hau
J
J
D000210:Acute-Phase Reaction / Q000276:immunology; D000818:Animals; D001798:Blood Proteins / Q000276:immunology; D002175:Candida / Q000166:cytology / Q000472:pathogenicity; D002177:Candidiasis / Q000276:immunology / Q000473:pathology*; D002417:Cattle; D050577:Complement C3-C5 Convertases / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000473:pathology; D008413:Mastitis / Q000276:immunology / Q000473:pathology*; D008414:Mastitis, Bovine / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D000511:alpha-Macroglobulins / Q000032:analysis
D001798:Blood Proteins; D000511:alpha-Macroglobulins; D050577:Complement C3-C5 Convertases