title: Effect of FK 506 administered topically versus intramuscularly on suppression of the corneal immune reaction in rats.
abstract: The immunosuppressive effect of FK 506 on penetrating keratoplasty in rats was evaluated following intramuscular and topical administration. Implanted corneal grafts were inspected weekly by clinical evaluation for 3 weeks. Cytotoxic T lymphocyte (CTL) activity was measured in the spleen on postoperative day 21, and the grafts were examined histologically. A dose of FK 506, 0.1 mg/kg given intramuscularly, only moderately suppressed CTL activity and the graft failed. In contrast, doses of either 0.024 mg/day topically or 0.3 mg/kg intramuscularly suppressed CTL activity and the grafts remained intact. Results suggest that FK 506 administered topically would be effective in preventing failure of human corneal grafts.
authors: Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
authors: K
authors: K
authors: Okada
authors: H
authors: H
authors: Sakata
authors: A
authors: A
authors: Minamoto
authors: M
authors: M
authors: Fujihara
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003315:Cornea / Q000276:immunology* / Q000473:pathology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D007273:Injections, Intramuscular; D015948:Keratoplasty, Penetrating / Q000276:immunology* / Q000473:pathology; D008297:Male; D009883:Ophthalmic Solutions; D051381:Rats; D011916:Rats, Inbred F344; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D016559:Tacrolimus / Q000008:administration & dosage*
chemical_list: D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D016559:Tacrolimus