The Thomsen-Friedenreich (T) simple mucin-type carbohydrate antigen in salivary gland carcinomas.
The simple mucin-type T (Thomsen-Friedenreich) antigen is a marker of carcinomas, and has been related to aggressiveness of malignant tumours. We studied the expression of T, sialosyl-T, A and H blood group antigens in salivary gland carcinomas. The aim was to study whether the tumours, based on the expression of these structures, could be divided into new diagnostic groups that may later show prognostic significance. Formalin fixed paraffin-embedded tissue sections from 77 salivary gland carcinomas of different histological types were studied using immunohistology and monoclonal antibodies (MAbs). Fresh frozen tissue was examined in 30 of the cases. Frozen sections were superior to paraffin sections in demonstrating T and H antigens. Aberrant glycosylation with accumulation of T (in cytoplasm) and sialosyl-T antigens (in cytoplasm, membrane and mucin) was found in all tumour types except acinic cell carcinomas. In carcinomas in pleomorphic adenomas (CinPA) the effect of fixation was minimal and T antigen location was different. In carcinomas with myoepithelial cell (MEC) participation, the MECs had retained a normal glycosylation pattern. H antigen was expressed in all tumour types, except acinic cell carcinomas and CinPA. A antigen was expressed in all tumour types from blood group A patients, except in CinPA. The expression of T, sialosyl-T, H and A antigens in relation to differentiation grade varied with tumour type in poorly differentiated areas. High and moderate differentiated areas were always stained, whereas poorly differentiated areas in some tumour types expressed T and sialosyl-T antigens and others did not. The accumulation versus lack of expression of the investigated structures in poorly differentiated areas of the tumours may be of prognostic significance.
Therkildsen
M H
MH
Department of Pathology Rigshospitalet, University of Copenhagen, Denmark.
Mandel
U
U
Christensen
M
M
Dabelsteen
E
E
D000017:ABO Blood-Group System / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis; D009077:Mucins / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000276:immunology* / Q000473:pathology
D000017:ABO Blood-Group System; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D007519:Isoantigens; D009077:Mucins; C087769:sialosyl-T antigen; C016467:Thomsen-Friedenreich antigen