title: Cytokine and coagulation characteristics of retrieved blood after arthroplasty.
abstract: OBJECTIVE
  To investigate cytokine and coagulation/fibrinolysis characteristics in blood retrieved from wounds using an autotransfusion system, and to compare the cytokine pattern in the retrieved blood with those in the systemic circulation and in the initial portion of drainage blood from the wound.
  
  
  METHODS
  Prospective controlled clinical study.
  
  
  METHODS
  The postoperative ward of a University hospital.
  
  
  METHODS
  Blood retrieval was performed over a period of 4-6 h on patients who had just undergone arthroplasty (nine hips, one knee). In five other cases involving hip arthroplasties, the initial portion of drainage blood was studied.
  
  
  RESULTS
  Coagulation/fibrinolysis parameters were analyzed in blood retrieved using the Stryker Consta Vac system. Concentrations of tumor necrosis factor alpha (TNF), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) were analyzed in the retrieved blood, in the systemic circulation of the patients at the beginning and at the end of blood retrieval and in the initial portion of drainage blood from the surgical area. In the retrieved blood, the activities of thrombin, kallikrein and plasmin were increased, antithrombin and free protein S were decreased, and in all samples IL-6 was >1000 pg/ml. Postoperative plasma concentrations of IL-6 rose from a median value of 0 to 116 pg/ml (p <0.01). Four patients had circulating TNF concentrations (range: <15-50 pg/ml). Plasma IL-1 was not detected. TNF and IL-1 were detected in all samples of initial blood from the surgical area and IL-6 in one sample.
  
  
  CONCLUSIONS
  Hypercoagulability and high concentrations of IL-6 were present in the retrieved blood. The cytokine pattern in the initial portion of blood from the surgical area differed from those in the retrieved blood and in the systemic circulation.
authors: Department of Anesthesiology, Karolinska Institute, Huddinge University Hospital, Sweden.
authors: M
authors: M
authors: Kristiansson
authors: M
authors: M
authors: Soop
authors: L
authors: L
authors: Saraste
authors: K G
authors: KG
authors: Sundqvist
authors: A M
authors: AM
authors: Suontaka
authors: M
authors: M
authors: Blomback
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001178:Arthroplasty; D001777:Blood Coagulation / Q000502:physiology*; D001804:Blood Transfusion, Autologous; D016207:Cytokines / Q000097:blood*; D005260:Female; D005342:Fibrinolysis / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha