Inhibition of protein synthesis in frog (Xenopus laevis) egg extracts by an antibody against phosphatidylinositol 4,5-bisphosphate.
The antibody kt10, which is directed against the phospholipid PtdIns(4,5)P2, inhibits protein synthesis when added to cytosolic extracts prepared from frog eggs. Addition of stable analogues of diacylglycerol and Ins(1,4,5)P3 failed to rescue the inhibition of translation, suggesting that the effect of the antibody was not to block hydrolysis of PtdIns(4,5)P2. Neomycin, which also binds PtdIns(4,5)P2, produced a similar reduction in protein-synthesis levels in the extract system, supporting the idea that it is the interaction of the antibody with PtdIns(4,5)P2 that is producing the effect.
Keating
T J
TJ
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
Fukami
K
K
Robinson
K R
KR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002457:Cell Extracts; D004527:Egg Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D009355:Neomycin / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000276:immunology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D004527:Egg Proteins; D011500:Protein Synthesis Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C068961:phosphatidylinositol 4,5-biphosphate kinase; D009355:Neomycin; D002118:Calcium