Effect of photorefractive keratectomy on the accuracy of pneumatonometer readings in rabbits.
OBJECTIVE
To determine whether measurement of intraocular pressure (IOP) using a pneumatonometer is reliable after myopic 5 or 15 D excimer laser photoablation in rabbits.


METHODS
Ten rabbits underwent 5 D myopic photorefractive keratectomy (PRK) of the left eye. Another seven rabbits underwent 15 D PRK: The right eye served as a control. The diameter of each PRK was 5 mm. Rabbits were examined 2.5 to 3 months later under general anesthesia. Eyes were cannulated, and the IOP was maintained at 5 to 40 mm Hg and measured using an intracameral manometer and a pneumatonometer at each pressure level; approximately 50 pressure points were formed. Readings of the two techniques were compared.


RESULTS
Linear regression analysis comparing manometric and pneumatonometric readings revealed the following data in eyes with 5 D corrections (n = 10): correlation coefficient (r) = 0.926, slope = 1.058, and intercept = -3.133. The values of the unoperated control eyes were: r = 0.900, slope = 0.962, and intercept = -1.010. The following results were obtained in eyes with 15 D photoablation (n = 7): r = 0.876, slope 1.133, and intercept -3.147. Values for the control eye were: r = 0.885, slope = 1.175, and intercept = -3.497. When the manometer and pneumatonometer readings of all animals were compared, the adjusted squared correlation coefficient was 79%. When the variabilities associated with the animals and the PRK procedure (pooled 5 and 15 D corrections) were taken into account, adjusted squared correlation coefficient increased from 8% to 87%.


CONCLUSIONS
Photorefractive keratectomy as high as 15 D/5 mm had only a minor effect on pneumatonometer readings in rabbits, indicating that the elastic properties of the cornea related to the accuracy of pneumatonometry were not significantly altered. Postoperative IOP monitoring with tonometers, based on flattening of the cornea under pressure, is accurate after PRK.
Tuunanen
T H
TH
University Eye Hospital, Helsinki, Finland.
Hämäläinen
P
P
Mali
M
M
Oksala
O
O
Tervo
T
T
D000818:Animals; D016001:Confidence Intervals; D005260:Female; D007429:Intraocular Pressure; D054018:Lasers, Excimer; D008297:Male; D009216:Myopia / Q000601:surgery; D018903:Photorefractive Keratectomy; D011817:Rabbits; D012044:Regression Analysis; D015203:Reproducibility of Results; D014065:Tonometry, Ocular