[The effect of selected polychlorinated hydrocarbon pesticides on proliferation of cells in rat liver (14 day experiment)].
The aim of present studies was to describe the effect of two organochlorine pesticides: nuarimol and DDT on the changes in rat liver, proposed in the literature to be useful endpoints in screening of non-genotoxic hepatocarcinogens and/or liver tumor promoters. The effects on the following endpoints: mitogenesis (DNA synthesis and mitotic activity), hepatomegaly as well as histological changes in rat liver have been investigated. Male Wistar rats received nuarimol or DDT in one, five and fourteen daily oral administration of the doses of 125 and 12 mg/kg b.w. day-1 respectively. In the case of both pesticides the effects observed consisted of hepatomegaly and hepatocyte proliferation (DNA synthesis and mitotic activity), although our studies indicated several distinct differences in the hepatic response between nuarimol, on the one hand and DDT on the other. The differences were reflected in the character and the basal rate of hepatocyte proliferation. Nuarimol elicited rapid but transient wave of hepatocyte proliferation during the first day of exposure. As opposite to nuarimol, DDT induced sustained hepatocyte proliferation during experimental period (14 days). Moreover, DDT induced evident focal necrosis and abnormal mitoses whereas nuarimol caused only slight vacuolated cytoplasm. Thus it can be concluded that nuarimol induced in rat liver direct mitogenic effect. On the other hand, DDT which is well known hepatocarcinogen, was found to produce mitogenic effect appearing to be related to regenerative response, since histological signs of necrosis were apparent.
Kostka
G
G
Zakład Toksykologii Srodowiskowej, Państwowy Zakład Higieny, Warszawa.
Kopeć-Szlezak
J
J
Palut
D
D
D000818:Animals; D003634:DDT / Q000633:toxicity*; D004261:DNA Replication / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008934:Mitogens / Q000633:toxicity; D008940:Mitotic Index / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D011743:Pyrimidines / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D007306:Insecticides; D008934:Mitogens; D011743:Pyrimidines; D003634:DDT; C067672:nuarimol
Wpływ wybranych pestycydów z grupy polichlorowanych weglowodorów na proliferacje komórkowa w watrobie szczurów (doświadczenie 14 dniowe).