Comparative efficiency of simple lipopeptide constructs for in vivo induction of virus-specific CTL.
We have previously shown that virus-specific CTL responses can be elicited in vivo by injecting, without adjuvant, 12-40 amino acid-long peptides, modified in C-terminal position by a simple lipidic amino acid. In this paper, we have studied the chemical accessibility, and the ability to induce in mice a CTL response, of a series of lipopeptides derived from the HIV-1 env (312-327) or (302-335) sequences. We showed that a single modification of these peptides by a lipidic amino acid, preferably in C-terminal position, results in the ability to reproducibly induce, without adjuvant, a relevant CTL response. No clear discrimination appeared concerning the nature of the lipidic modification. Our findings indicate that modification of a relatively long peptide by a N epsilon-palmitoyl-L-Lysylamide can be achieved by conventional methods of synthesis and characterization, offering the possibility to develop low-cost synthetic vaccines in models in which the CTL component is of importance.
Deprez
B
B
Laboratoire de Chimie des Biomolécules, Université de Lille II, URA, CNRS 1309, Institut Pasteur de Lille, France.
Sauzet
J P
JP
Boutillon
C
C
Martinon
F
F
Tartar
A
A
Sergheraert
C
C
Guillet
J G
JG
Gomard
E
E
Gras-Masse
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015686:Gene Products, env / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D015686:Gene Products, env; D008074:Lipoproteins; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; D014765:Viral Vaccines