Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: detection of HCV RNA in archival sera.
A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome.
Beardsley
A M
AM
Infectious Diseases Laboratory, Institute of Medical and Veterinary Science, University of Adelaide, Australia.
Gowans
E J
EJ
Burrell
C J
CJ
Marmion
B P
BP
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005069:Evaluation Studies as Topic; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology; D006801:Humans; D008979:Moloney murine leukemia virus / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis / Q000821:virology; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D018076:DNA, Complementary; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase