Immunohistochemical characterization of canine transmissible venereal tumor.
The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains,canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T-and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.
Mozos
E
E
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria de Córdoba, Spain.
Méndez
A
A
Gómez-Villamandos
J C
JC
Martín De Las Mulas
J
J
Pérez
J
J
D000818:Animals; D017252:CD3 Complex / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism; D007075:Immunoglobulin M / Q000032:analysis / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007633:Keratins / Q000032:analysis / Q000378:metabolism; D008297:Male; D009113:Muramidase / Q000032:analysis / Q000378:metabolism; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism; D014685:Venereal Tumors, Veterinary / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D014746:Vimentin / Q000032:analysis / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000378:metabolism
D017252:CD3 Complex; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009418:S100 Proteins; D014746:Vimentin; D000515:alpha 1-Antitrypsin; D007633:Keratins; D009113:Muramidase