The A1 x U72 base pair conserved in eukaryotic initiator tRNAs is important specifically for binding to the eukaryotic translation initiation factor eIF2.
The formation of a specific ternary complex between eukaryotic initiation factor 2 (eIF2), the initiator methionyl-tRNA (Met-tRNA), and GTP is a critical step in translation initiation in the cytoplasmic protein-synthesizing system of eukaryotes. We show that the A1 x U72 base pair conserved at the end of the acceptor stem in eukaryotic and archaebacterial initiator methionine tRNAs plays an important role in this interaction. We changed the A1 x U72 base pair of the human initiator tRNA to G1 x C72 and expressed the wild-type and mutant tRNA genes in the yeast Saccharomyces cerevisiae by using constructs previously developed in our laboratory for expression of the human initiator tRNA gene in yeasts. We show that both the wild-type and mutant human initiator tRNAs are aminoacylated well in vivo. We have isolated the wild-type and mutant human initiator tRNAs in substantially pure form, free of the yeast initiator tRNA, and have analyzed their properties in vitro. The G1 x C72 mutation affects specifically the binding affinity of eIF2 for the initiator tRNA. It has no effect on the subsequent formation of 40S or 80S ribosome initiator Met-tRNA-AUG initiation complexes in vitro or on the puromycin reactivity of the Met-tRNA in the 80S initiation complex.
Farruggio
D
D
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Chaudhuri
J
J
Maitra
U
U
RajBhandary
U L
UL
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D012358:RNA, Transfer, Met / Q000737:chemistry* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship; D046249:Transfer RNA Aminoacylation
D017931:DNA Primers; D015852:Eukaryotic Initiation Factor-2; D012358:RNA, Transfer, Met; D016601:RNA-Binding Proteins; D008718:Methionine-tRNA Ligase