Morphologic differences between latent membrane protein-1 (LMP-1)-positive and negative tumour cells in Epstein-Barr virus (EBV)-related childhood Hodgkin's disease. A morphometric study.
The values of five cellular morphometric parameters (longest and shortest cytoplasmic axis, cellular circumference, area and roundness coefficient) were compared between 20 Latent Membrane Protein 1 (LMP-1)-positive and an equal number of LMP-1-negative Reed-Sternberg and Hodgkin (HRS) cells for each of 13 cases of Hodgkin's disease (HD) occurring in children (aged 3-15 years); the presence of Epstein-Barr virus (EBV) encoded EBER mRNAs had previously been detected in all cases using RNA in situ hybridisation (RISH), while the presence of LMP-1 was immunohistochemically detected using the alkaline phosphatase-antialkaline phosphatase (APAAP) method. The longest and shortest axis, circumference and area were larger in LMP-1-positive than in LMP-1 negative HRS cells, while the roundness coefficient of LMP-positive HRS cells was smaller than that of LMP-1 negative cells. All differences were statistically highly significant when univariate (paired comparisons) t-test were used. Multivariate analysis (Hotelling's T2 test) showed all differences (except the roundness coefficient) to be significant both at the 5% and 1% level of significance. These results provide a numerical basis for the alteration brought by the expression of LMP-1 in the cellular skeleton of tumour (HRS) cells in EBV-related childhood HD cases.
Panayiotides
J
J
Department of Pathology, Metaxas Memorial Cancer Hospital, Piraeus, Greece.
Kanavaros
P
P
Protopapa
E
E
Vlachonikolis
J
J
Tzardi
M
M
Kalmanti
M
M
Delides
G
G
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D006566:Herpesviridae Infections / Q000378:metabolism / Q000473:pathology*; D004854:Herpesvirus 4, Human; D006689:Hodgkin Disease / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D006801:Humans; D014412:Tumor Virus Infections / Q000378:metabolism / Q000473:pathology*; D014763:Viral Matrix Proteins / Q000032:analysis*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins