Permeability of nitric oxide through lipid bilayer membranes.
Profiles of the local nitric oxide (.NO) diffusion-concentration product across the egg yolk phosphatidylcholine membrane in the absence and presence of 30 mol% cholesterol were obtained using line-broadening electron paramagnetic resonance (EPR) and lipid-soluble nitroxide spin labels. Membrane .NO permeability coefficients were calculated from these profiles. At 20 degrees C, values of 93 and 77 cm/s for membranes in the absence and presence of cholesterol were obtained, compared with 73 and 66 cm/s for water layers of the same thickness as the membranes. Fluid-phase membranes are not barriers to .NO transport. Cholesterol significantly increases .NO transport in the center of the lipid bilayer.
Subczynski
W K
WK
Department of Biophysics, Jagiellonian University, Krakow, Poland.
Lomnicka
M
M
Hyde
J S
JS
D001692:Biological Transport; D004058:Diffusion; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers; D015335:Molecular Probes; D009569:Nitric Oxide / Q000493:pharmacokinetics*; D010539:Permeability; D010713:Phosphatidylcholines
D008051:Lipid Bilayers; D015335:Molecular Probes; D010713:Phosphatidylcholines; D009569:Nitric Oxide