Synaptotagmin II immunoreactivity in normal and botulinum type-A treated mouse motor nerve terminals.
The distribution of synaptotagmin II, a synaptic vesicle protein, was examined by immunohistochemistry at normal mouse motor nerve terminals and after botulinum type-A treatment. An immunoreactivity to synaptotagmin II was detected in both control and botulinum type-A treated motor nerve terminals including newly formed sprouts. These data, together with other reports showing the absence of synaptotagmin I at the neuromuscular junction, suggest that synaptotagmin II is the isoform involved in transmitter release at motor nerve terminals.
Juzans
P
P
Laboratoire de Neurobiologie Cellulaire et Moléculaire, C.N.R.S., Gif-sur-Yvette, France.
Molgo
J
J
Faille
L
L
Angaut-Petit
D
D
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009045:Motor Endplate / Q000187:drug effects* / Q000378:metabolism*; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism; D050861:Synaptotagmin II
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050861:Synaptotagmin II; C494263:Syt2 protein, mouse; D019274:Botulinum Toxins, Type A; D000109:Acetylcholine