Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome.
OBJECTIVE
To assess the potential involvement of cytokines and nitrites in the hyperpermeability characterizing the ovarian hyperstimulation syndrome (OHSS).


METHODS
A controlled clinical study comparing peritoneal fluid (PF) from patients with severe OHSS and from non-OHSS controls.


METHODS
Women hospitalized with severe OHSS in three tertiary medical centers.


METHODS
Twelve patients with severe OHSS necessitating paracentesis and 20 non-OHSS controls.


METHODS
The criteria for ultrasound-guided paracentesis were tense ascites, hydrothorax, hemoconcentration, or oliguria.


METHODS
Interleukin (IL) 1 beta IL-1 receptor agonist, IL-2, IL-6, IL-8, and tumor necrosis factor alpha (TNF alpha) levels in PF were assayed by ELISA; nitrites were measured by the "Griess" reaction. Estradiol and P were determined by RIA.


RESULTS
Ovarian hyperstimulation syndrome patients had significantly higher PF IL-6 (3,523 versus 30 pg/mL), TNF alpha (14 versus 4.2 pg/mL), and IL-8 (1,695 versus 900 pg/mL). In the serum, only IL-6 levels were significantly higher (375 versus 11 pg/mL). Conversely, nitrite levels were significantly lower in PF of OHSS patients (0.5 versus 34 nmol/mL). Interleukin 1 levels were higher and IL-1 receptor antagonist levels were lower in OHSS patients, suggesting potentially increased biologic potency of IL-1.


CONCLUSIONS
These findings suggest that these substances could be involved in mediating the capillary hyperpermeability characterizing this syndrome.
Revel
A
A
Department of Obstetrics and Gynecology, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel. revel@.yam-suff.cc.huji.ac.il
Barak
V
V
Lavy
Y
Y
Anteby
E
E
Abramov
Y
Y
Schenker
J J
JJ
Amit
A
A
Finci-Yeheskel
Z
Z
Mayer
M
M
Simon
A
A
Laufer
N
N
Hurwitz
A
A
D001202:Ascitic Fluid / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D016471:Ovarian Hyperstimulation Syndrome / Q000378:metabolism*
D016207:Cytokines; D007378:Interleukins; D009566:Nitrates; D009573:Nitrites