Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
Petitte, J.N., Clarck, M.E., Verrinder Gibbins, A. M. and R. J. Etches (1990; Development 108, 185-189) demonstrated that chicken early blastoderm contains cells able to contribute to both somatic and germinal tissue when injected into a recipient embryo. However, these cells were neither identified nor maintained in vitro. Here, we show that chicken early blastoderm contains cells characterised as putative avian embryonic stem (ES) cells that can be maintained in vitro for long-term culture. These cells exhibit features similar to those of murine ES cells such as typical morphology, strong reactivity toward specific antibodies, cytokine-dependent extended proliferation and high telomerase activity. These cells also present high capacities to differentiate in vitro into various cell types including cells from ectodermic, mesodermic and endodermic lineages. Production of chimeras after injection of the cultivated cells reinforced the view that our culture system maintains in vitro some avian putative ES cells.
Pain
B
B
Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon, UMR 49 CNRS/ENS, France. bpain@ens-lyon.fr
Clark
M E
ME
Shen
M
M
Nakazawa
H
H
Sakurai
M
M
Samarut
J
J
Etches
R J
RJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001756:Blastoderm / Q000166:cytology / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002642:Chick Embryo; D003370:Coturnix / Q000196:embryology; D016207:Cytokines / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology; D006131:Growth Inhibitors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D019098:Telomerase / Q000378:metabolism; D014212:Tretinoin / Q000276:immunology / Q000494:pharmacology
D000906:Antibodies; D016207:Cytokines; D000939:Epitopes; D006131:Growth Inhibitors; D006133:Growth Substances; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D014212:Tretinoin; D019098:Telomerase; D000469:Alkaline Phosphatase