title: [Dynamic changes of amino acids in extracellular fluid in rat hippocampus in cases of cerebral ischemia].
abstract: OBJECTIVE
  To study the changes of excitatory amino acid (EAA) in patients with cerebral ischemia.
  
  
  METHODS
  We observed dynamic changes of EAA in extracellular fluid by intracerebral microdialysis inserted in hippocampus through stereotaxic method with modified Pulsinelli's model of four vessels occlusion.
  
  
  RESULTS
  The concentration of Glu was 5.88 +/- 1.40 and 11.2 +/- 1.5 micromol/L in patients with incomplete cerebral ischemia after 30 minutes and 60 minutes, and the concentration of Asp was 2.72 +/- 0.24 and 4.4 +/- 0.6 micromol/L. The concentration of Glu was 15.1 +/- 1.6 micromol/L and 17.9 +/- 1.6 micromol/L in patients with complete cerebral ischemia after 30 minutes and 60 minutes, and the concentration of Asp was 8.2 +/- 1.0 and 12.1 +/- 1.1 micromol/L. The concentration of Glu and Asp was 1.75 +/- 0.88 micromol/L and 0.24 +/- 0.09 micromol/L in the controls.
  
  
  CONCLUSIONS
  In this study, the concentration of Glu and Asp in patients with cerebral ischemia was significantly increased than in the controls, and the more serious ischemia was, the more release of EAA. It is suggested that Dansen injection can reduce the release of EAA by cerebral cells in case of ischemia.
authors: Institute of Neurology, Hunan Medical University, Changsha.
authors: J
authors: J
authors: Li
authors: Q
authors: Q
authors: Yang
authors: S
authors: S
authors: Ouyang
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D005973:Glutamine