Nucleotide sequence of the variable region of the heavy and light chains of a monoclonal IgG antibody reactive to herbicides, terbutryn and prometryn.
Members of the triazine family of herbicides are reliable indicators of contamination of the ground water or soil with pesticide residues. To facilitate better detection of the chemical residues using improved immunoassay procedures, several monoclonal antibodies against triazine herbicides have been developed. K1F4 is a hybridoma secreting monoclonal (IgG) antibody reactive to terbutryn and prometryn, two members of the triazine family. We have cloned the genes encoding the variable regions of the heavy and light chains of this monoclonal antibody and report the nucleotide sequence here.
Kreissig
S B
SB
Antibody Engineering Laboratory and Department of Entomology, University of California, Davis, CA 95616 USA.
Ward
V K
VK
Hammock
B D
BD
Choudary
P V
PV
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D006540:Herbicides / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D011400:Prometryne / Q000276:immunology*; D014227:Triazines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006540:Herbicides; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D014227:Triazines; D011400:Prometryne; C010347:terbutryne