Studies of the platelet filter test (shear dependent platelet aggregation) in patients with uncommon haemorrhagic disorders.
Platelets of anticoagulated whole blood forced at 40 mmHg through a fine filter are activated, aggregated and retained, so block the filter (platelet filter test, O'Brien JR, Salmon GP. Blood 1987; 1354-1361). Our clinical experiences with this simple and quick haemostasis test are summarized. Patients were investigated with different types of vWD (type-1 = 35, type-2A = 7, type-2B = 7, type-3 = 1), Glanzmann's thrombasthenia, congenital deficiency of cyclo-oxygenase, acquired Bernard-Soulier syndrome, FXII-, FXIII-deficiency and a control group. The cumulative drop count and the platelet retention were carefully measured during two phases of the filter test. Platelet count, bleeding time, vWF:Ag and vWF:Rcof activity were measured along with the platelet filter test. The filter was not blocked and the platelet retention was abnormally low in all patients with thrombasthenia, type-2a, type-2B, type-3 vWD. Treatment with 1-desamino-8-D-arginine-vasopressin (DDAVP) caused enhanced platelet retention in 16 patients with type-1 vWD. The test is simple, quick and cheap, has good reproducibility, and may be useful in clinical haemostasis laboratories for examination of high shear induced platelet functions.
Boda
Z
Z
Second Department of Internal Medicine, University Medical School, Debrecen, Hungary.
Tornai
I
I
Rak
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D003894:Deamino Arginine Vasopressin / Q000627:therapeutic use; D005175:Factor XII Deficiency / Q000097:blood; D005177:Factor XIII Deficiency / Q000097:blood; D005260:Female; D006474:Hemorrhagic Disorders / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D013915:Thrombasthenia / Q000097:blood; D014842:von Willebrand Diseases / Q000097:blood
D003894:Deamino Arginine Vasopressin