title: Genetic analysis of oxygen defense mechanisms in Drosophila melanogaster and identification of a novel behavioural mutant with a Shaker phenotype.
abstract: Mutants of Drosophila melanogaster that lack Cu/Zn superoxide dismutase or urate are hypersensitive to reactive oxygen species (ROS) generated in vivo by the redox-cycling agent paraquat. We have subsequently employed paraquat as a selective agent to identify adult viable mutants potentially defective in other, perhaps unknown, components of ROS metabolism. Paraquat screening of ethyl methanesulfonate-induced second- and third-chromosome mutations yielded 24 paraquat hypersensitive mutants. Two mutants were identified as being new alleles of the previously identified doublesex (dsx) and pink (p) genes. The remainder of the mutations identified previously undescribed genes, including one second chromosome paraquat hypersensitive mutant that was found to exhibit shaking legs, abdomen pulsations, and body shuddering under ether anaesthesia. This recessive mutation was mapped to the polytene chromosome region of 48A5-48B2 and defines a new gene we named quiver (qvr). This mutation is similar in phenotype to the Shaker (Sh), ether-a-gogo (eag), and Hyperkinetic (Hk) mutations, all of which affect potassium channel function in D. melanogaster.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, ON, Canada.
authors.forename: J M
authors.initials: JM
authors.lastname: Humphreys
authors.forename: B
authors.initials: B
authors.lastname: Duyf
authors.forename: M L
authors.initials: ML
authors.lastname: Joiner
authors.forename: J P
authors.initials: JP
authors.lastname: Phillips
authors.forename: A J
authors.initials: AJ
authors.lastname: Hilliker
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005260:Female; D005786:Gene Expression Regulation; D005804:Genes, Lethal; D006579:Heterozygote; D007301:Insect Hormones / Q000235:genetics; D008136:Longevity / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D009069:Movement Disorders / Q000235:genetics*; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C070369:DSX protein, Drosophila; D029721:Drosophila Proteins; D007301:Insect Hormones; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D005020:Ethyl Methanesulfonate; D013482:Superoxide Dismutase