[Modification of a method for studying free intracellular calcium].
A microspectrofluorimeter which permits recording excitation and emission spectra of fluorescent dyes (fura-2, indo-1) and measuring Ca(2+)-transients in different cells and smooth muscle strips is described. One-beam and two-beam approaches of recording of spectral characteristic of fluorescent dyes are presented.
Zyma
V L
VL
Diachok
O M
OM
Hanchurin
V V
VV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002477:Cells / Q000378:metabolism*; D004867:Equipment Design; D005456:Fluorescent Dyes; D009132:Muscles / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes; D002118:Calcium
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