Analysis of SRF, SAP-1 and ELK-1 transcripts and proteins in human cell lines.
We have analysed the expression of the genes encoding transcription factors involved in c-fos transcriptional regulation, i.e. the serum response factor (SRF) and the ETS-related proteins ELK-1 and SAP-1, in a variety of human cell lines. RNA was determined by Northern blot analysis, and proteins were detected on Western blots: the two analyses gave essentially identical results. SRF was expressed at similar levels in all cell lines tested. In contrast, SAP-1 and ELK-1 expression varied from one cell line to another. Interestingly, in any given cell line, high levels of one protein were accompained by low levels of the other. Similar results were obtained by electro-mobility shift assays (EMSA), using antibodies directed against the proteins. Thus, our data raise the possibility of a coordinated regulation of the expression of these two Ets genes, at both RNA and protein levels.
Magnaghi-Jaulin
L
L
Laboratorie de Biologie des Tumeurs Humaines, CNRS URA 1156, Villejuif, France.
Masutani
H
H
Lipinski
M
M
Harel-Bellan
A
A
D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D026362:Serum Response Factor; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics; D014407:Tumor Cells, Cultured; D050807:ets-Domain Protein Elk-1; D050808:ets-Domain Protein Elk-4
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C494112:ELK1 protein, human; C494193:ELK4 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D026362:Serum Response Factor; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D050808:ets-Domain Protein Elk-4