Formation of acarbose phosphate by a cell-free extract from the acarbose producer Actinoplanes sp.
The alpha-glucosidase inhibitor acarbose is modified during incubation with cell-free extract from the producing Actinoplanes strain. The formation of this product depends on the presence of ATP. Chromatographic and chemical properties of the purified transformation product indicate the presence of a phosphate ester. The structure is deduced by NMR analysis and shown to be acarbose-7-phosphate.
Goeke
K
K
Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Münster, Germany.
Drepper
A
A
Pape
H
H
D020909:Acarbose; D000192:Actinomycetales / Q000378:metabolism*; D002457:Cell Extracts; D004791:Enzyme Inhibitors / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010710:Phosphates / Q000378:metabolism; D014312:Trisaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D002457:Cell Extracts; D004791:Enzyme Inhibitors; D010710:Phosphates; D014312:Trisaccharides; C101443:acarbose 7-phosphate; D020909:Acarbose