Lignan models as inhibitors of Phanerochaete chrysosporium lignin peroxidase.
The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited these enzymes to some extent. If the double bonds were hydrogenated, the inhibitory effect was lost. HRP-VIII and HRP-XI were slightly inhibited by BMDCA (I0.5 > 50 microM) and two plant peroxidases described as efficient lignan synthesizers were unaffected. Liquid cultures of P chrysosporium did not discolour the dve Poly R478 when 250 microM of BMDCA was present.
Frías
I
I
Departamento de Bioquímica, Universidad de La Laguna, Tenerife Canary Islands, Spain.
Trujillo
J M
JM
Romero
J
J
Hernandez
J
J
Pérez
J A
JA
D000880:Anthraquinones; D001487:Basidiomycota / Q000201:enzymology*; D001592:Benzyl Alcohols / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D004396:Coloring Agents; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors; D017705:Lignans / Q000494:pharmacology*; D010544:Peroxidases / Q000037:antagonists & inhibitors*; D011108:Polymers
D000880:Anthraquinones; D001592:Benzyl Alcohols; D002934:Cinnamates; D004396:Coloring Agents; D017705:Lignans; D011108:Polymers; C017971:poly R-478; D006735:Horseradish Peroxidase; D010544:Peroxidases; C042858:lignin peroxidase; C042197:veratryl alcohol