In vitro propagation of Rauvolfia serpentina through tissue culture.
Multiple shoots were induced from nodal segments and shoot apices of Rauvolfia serpentina and MS medium containing 1.0 mg/l BA and 0.1 mg/l NAA was found to give the best shoot proliferation rate. Callus formed at cut bases of the explants which produced shoots when subcultured on media containing low concentration of BA (0.5 or 0.1 mg/l) and NAA (0.1 mg/l). The in vitro proliferated shoots were rooted and later transferred to the soil.
Sarker
K P
KP
Islam
A
A
Islam
R
R
Hoque
A
A
Joarder
O I
OI
D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010946:Plants, Medicinal; D011926:Rauwolfia / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D007210:Indoleacetic Acids; D007211:Indoles; D009280:Naphthaleneacetic Acids; C014612:indolebutyric acid; C034182:1-naphthaleneacetic acid