title: Review: biosynthesis and function of yeast vacuolar proteases.
abstract: The yeast vacuole, which is equivalent to the lysosome of higher eukaryotes, is one of the best characterized degradative organelles. This review describes the biosynthesis and function of yeast vacuolar proteases. Most of these enzymes are delivered to the vacuole via the early compartments of the secretory pathway and the endosome, while one of them is directly imported from the cytoplasm. The proteases are synthesized as precursors which undergo many post-translational modifications before the final active form is generated. Proteolytic activation by developments in the analysis of the functions of vacuolar proteolysis are described. Substrates of the vacuolar proteases are mostly imported via endocytosis or autophagocytosis, and vacuolar proteolysis appears to be mainly important under nutritional stress conditions and sporulation.
authors.affiliation: Department of Yeast Genetics, Carlsberg Laboratory, Copenhagen-Valby, Denmark.
authors.forename: H B
authors.initials: HB
authors.lastname: Van Den Hazel
authors.forename: M C
authors.initials: MC
authors.lastname: Kielland-Brandt
authors.forename: J R
authors.initials: JR
authors.lastname: Winther
mesh_terms: D000595:Amino Acid Sequence; D001693:Biological Transport, Active; D004705:Endocytosis; D010450:Endopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity; D014617:Vacuoles / Q000201:enzymology; D015003:Yeasts / Q000201:enzymology* / Q000235:genetics
chemical_list: D004792:Enzyme Precursors; D010450:Endopeptidases