title: Quality control of histamine and methacholine in diagnostic solutions with capillary electrophoresis.
abstract: Solutions of histamine and methacholine bromide in different matrices for diagnostic purposes were analyzed for stability and quality control using capillary electrophoresis. Histamine (2,[4-imidazolyl]ethylamine) [CAS No. 51-45-6] was determined using a 0.1 M Tris-borate buffer of pH 8.3 with 5.10(-5) M cetyltrimethylammonium bromide (CTAB) and 0.005% poly(vinyl alcohol) (PVA) and detected at 214 nm using clenbuterol [4-amino-alpha-(tert.-butylaminomethyl)-3,5-dichlorobenzyl alcohol] [37148-27-9] as an internal standard. Metacholine bromide (acetyl-beta-methacholine bromide) [333-31-3] was determined with a 0.01 M creatinine-chloride buffer of pH 4.85 and detected with indirect UV at 230 nm using potassium as an internal standard. Histamine solutions were stable for a prolonged period of time, whereas under enforced degradation conditions methacholine was hydrolyzed, yielding acetic acid and (tentatively) beta-methylcholine as reaction products.
authors.affiliation: Laboratory of Instrumental Analysis, Eindhoven University of Technology, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: van der Schans
authors.forename: J C
authors.initials: JC
authors.lastname: Reijenga
authors.forename: F M
authors.initials: FM
authors.lastname: Everaerts
mesh_terms: D004355:Drug Stability; D019075:Electrophoresis, Capillary; D006632:Histamine / Q000032:analysis*; D008688:Methacholine Compounds / Q000032:analysis*; D011786:Quality Control; D012996:Solutions
chemical_list: D008688:Methacholine Compounds; D012996:Solutions; D006632:Histamine