title: Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.
abstract: An approach for the simultaneous acquisition of HCN and HCP as well as HCN-CCH-TOCSY and HCP-CCH-TOCSY triple resonance data sets for 13C-/15N-labelled RNAs is presented. The new HCN-CCH-TOCSY scheme unambiguously links all sugar resonances to the base nitrogen. In addition, simultaneous acquisition of HCN-CCH-TOCSY and HCP-CCH-TOCSY data sets provides sequential and base-type information in a single experiment, thereby saving data acquisition time as well as providing complementary data sets that are useful in clarifying ambiguous assignments. Virtually complete sequence-specific phosphate-ribose 1H, 31P, and base 15N1,9, assignments as well as partial 13C assignments could be obtained in a single experiment for a 0.5-mM sample of a 19-mer ribonucleotide.
authors.affiliation: Institut für Molekulare Biotechnologie, Jena, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Ramachandran
authors.forename: C
authors.initials: C
authors.lastname: Sich
authors.forename: M
authors.initials: M
authors.lastname: Grüne
authors.forename: V
authors.initials: V
authors.lastname: Soskic
authors.forename: L R
authors.initials: LR
authors.lastname: Brown
mesh_terms: D001483:Base Sequence; D002247:Carbon Isotopes; D003627:Data Interpretation, Statistical; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D012313:RNA / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D012313:RNA