title: Cytotoxicity of NaCl, a stomach tumor promoter, and prevention by rice extract in stomach mucosa of F344 rats.
abstract: Cytotoxicity of NaCl and its prevention by rice extract were studied in the pyloric mucosa of male F344 rat stomach after oral administration of rice extract and 2.6 M NaCl. Effect were observed histologically by hematoxylin and eosin staining and the bromodeoxyuridine method. Replicative DNA synthesis (RDS) was assayed by liquid scintillation counter with [3H]thymidine. NaCl (2.6 M) induced destruction of the surface mucous cells within 1 min. RDS and S-phase cells increased significantly (p < 0.01) and to a maximum at 17 h, and returned to control levels 48 h after exposure. Administration of aqueous rice extract 3 h before NaCl exposure reduced the morphological damage to the mucosa and prevented the increase in RDS dose dependently by up to 65% (p < 0.01). These results showed that NaCl induced rapid mucosal damage and cell proliferation in rat stomach mucosa and that rice extract prevented the damage and reduced the increase in RDS.
authors.affiliation: Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Furihata
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Tokuyama
authors.forename: T
authors.initials: T
authors.lastname: Katsuyama
authors.forename: Z
authors.initials: Z
authors.lastname: Ogita
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004261:DNA Replication; D008297:Male; D012275:Oryza; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D016196:S Phase; D012965:Sodium Chloride / Q000633:toxicity*; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D012965:Sodium Chloride