Immunoblot analysis of immune response to cell surface antigens of different Helicobacter pylori strains.
Infection with H. pylori has been strongly associated with chronic type B gastritis and peptic ulcer disease, although pathogenic mechanisms of H. pylori infection are still unclear. We examined the structure and antigenicity of water extract of 5 H. pylori strains and immunoblotting with homologous immune rabbit serum. All the tested rabbit sera recognized the protein component between 31-66 kD. The 31-66 kD protein bands are highly specific for H. pylori. Only strain isolated from patients with gastric or duodenal ulcer disease gave visible bands with the major proteins 98, 116 kD.
Karczewska
E
E
Institute of Physiology, University Medical School, Cracow, Poland.
Wyle
F
F
Tarnawski
A
A
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D004381:Duodenal Ulcer / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D011817:Rabbits; D013276:Stomach Ulcer / Q000382:microbiology
D000942:Antigens, Bacterial