title: [HPV-5 typing with nested PCR and sequencing in epidermodysplasia verruciformis].
abstract: We report a case of epidermodysplasia verruciformis (EV) with typical cutaneous manifestations in light-exposed areas. The diagnostic criteria were the characteristic macular lesions, the virus transformed keratinocytes on histological examination and the detection of human papillomavirus (HPV) DNA by a nested-PCR approach for EV-associated types. In this case we found HPV-type 5, the most common type of HPV in EV, directly by sequencing and sequence analysis.
authors.affiliation: Klinik für Dermatologie und Allergologie, St. Barbara-Hospital, Duisburg.
authors.forename: J
authors.initials: J
authors.lastname: Schaller
authors.forename: A
authors.initials: A
authors.lastname: Rohwedder
authors.forename: M
authors.initials: M
authors.lastname: Fuchs
authors.forename: A
authors.initials: A
authors.lastname: Maron
authors.forename: J
authors.initials: J
authors.lastname: Kunze
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D001706:Biopsy; D004279:DNA, Viral / Q000235:genetics; D004819:Epidermodysplasia Verruciformis / Q000473:pathology* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D012867:Skin / Q000473:pathology; D014412:Tumor Virus Infections / Q000473:pathology* / Q000821:virology
chemical_list: D004279:DNA, Viral
vernacular_title: HPV-5-Typisierung mittels nested PCR und Sequenzierung bei einer Epidermodysplasia verruciformis.