title: Influence of dietary fat on the effect of endotoxin on murine hepatic peroxisomes.
abstract: This study investigates the effects of either a high-fat diet or endotoxin on peroxisome metabolism as assessed by measuring catalase activity, catalase mass, and peroxisomal beta-oxidation. Three mouse strains C3H/HeJ, BALB/c, and C57BL/6J were fed either a low-fat or a high-fat diet and injected intraperitoneally with 1 microg Escherichia coli lipopolysaccharide. These parameters were not different in C3H/HeJ mice fed a high-fat diet compared with controls fed a low-fat diet. Total liver catalase activity and peroxisomal beta-oxidation were higher in BALB/c mice fed high-fat diet compared with low-fat controls. Total liver catalase activity, catalase mass and peroxisomal beta-oxidation increased to the greatest extent in C57BL/6J mice fed a high-fat diet. Endotoxin treatment did not alter any of the parameters in mice fed a low-fat diet. Among mice fed a high-fat diet, endotoxin did not affect hepatic catalase or peroxisomal beta-oxidation in the C3H/HeJ mice, decreased catalase activity in BALB/c mice (28%), and greatly decreased both catalase (66%) and peroxisomal beta-oxidation (69%) in C57BL/6J mice. The decrease in catalase activity in C57BL/6J mice was apparently because of an inactivation of the enzyme as determined by the activity/mass ratio. Thus, endotoxin is showed to inhibit both catalase and peroxisomal beta-oxidation in mice and the sensitivity to endotoxin is greatest in C57BL/6J mice fed a high-fat diet.
authors.affiliation: Louisiana State University Medical Center, Department of Pathology, New Orleans, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: DeLamatre
authors.forename: J T
authors.initials: JT
authors.lastname: Schilleci
authors.forename: L H
authors.initials: LH
authors.lastname: Hanson
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004731:Endotoxins / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008830:Microbodies / Q000187:drug effects*; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D004731:Endotoxins; D002374:Catalase