title: The use of the divalent calcium-ionophore A23187 as a biochemical tool in pharmacological and in vitro toxicological studies.
abstract: The use of A23187 as a biochemical tool is described. A23187 was used to elucidate the mechanisms of intracellular transport and secretion of N-hydroxylated aniline metabolites in rat hepatocyte primary culture. Results indicate a membrane-bound intracellular transport. Also, A23187 induced macrophages in their anti-tumour cytostatic activity using P815 tumour cells in in vitro co-cultures of macrophages and tumour cells. Results indicate the activating role of A23187 in macrophage leukotriene C4 release and enhanced macrophage anti-tumour activity. In conclusion, A23187 proved to be a useful tool in studying calcium dependent metabolic processes.
authors.forename: J H
authors.initials: JH
authors.lastname: Boot
authors.forename: J A
authors.initials: JA
authors.lastname: Van Hilten
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D007476:Ionophores / Q000633:toxicity*; D008099:Liver / Q000166:cytology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008985:Monensin / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000814:Aniline Compounds; D002273:Carcinogens; D007476:Ionophores; D000001:Calcimycin; D008985:Monensin; C023650:aniline; D003078:Colchicine; D002118:Calcium