An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricles.
Sensory epithelia from normal rat utricles and those cultured with and without neomycin treatment were assayed for the presence of growth factor receptor mRNAs by RT-PCR (reverse transcriptase-polymerase chain reaction). Both undamaged and damaged utricles showed mRNA for Insulin receptor, IGF-I receptor, FGF receptor 1, EGF receptor, and PDGF alpha receptor. Neomycin-damaged sensory epithelia showed less PDGF alpha receptor mRNA than undamaged epithelia, suggesting that this message by expressed at higher copy levels in hair cells than in supporting cells. Consistent with that hypothesis, immunohistochemistry revealed much stronger PDGF alpha receptor staining in the hair cells than in the supporting cells. Preliminary evidence suggests that IGF-I receptor message also may be lowered in neomycin-damaged epithelia.
Saffer
L D
LD
Department of Otolaryngology-HNS, University of Virginia, School of Medicine, Charlottesville 22908, USA.
Gu
R
R
Corwin
J T
JT
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D001483:Base Sequence; D002645:Chickens; D004848:Epithelium / Q000187:drug effects / Q000293:injuries; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005260:Female; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000293:injuries / Q000378:metabolism*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009355:Neomycin / Q000633:toxicity; D015345:Oligonucleotide Probes; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D020796:Receptor, Platelet-Derived Growth Factor alpha; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D012444:Saccule and Utricle / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism; D016707:Tissue Fixation
D000900:Anti-Bacterial Agents; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D066246:ErbB Receptors; D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin; D020796:Receptor, Platelet-Derived Growth Factor alpha; D017479:Receptors, Platelet-Derived Growth Factor; D009355:Neomycin