title: Murine T-lymphocytes express vasoactive intestinal peptide receptor 1 (VIP-R1) mRNA.
abstract: Vasoactive intestinal peptide (VIP), a neuropeptide present in primary and secondary lymphoid organs has been previously reported to inhibit IL-2 and IL-4 production as well as the proliferation of mitogen- or antigen-stimulated T-cells. Binding studies suggested that the immunoregulatory effects of VIP are mediated through specific VIP-binding sites present on lymphocyte subpopulations. Here we report on the expression of VIP-R1 mRNA in various murine lymphocyte subpopulations. By using RT-PCR. RNase protection assay, cDNA cloning, and sequence analysis, we show that stimulated and unstimulated murine spleen cells, thymocytes. CD4+ and CD8+ T-cells express VIP-R1. The VIP-R1 fragment amplified from murine brain, thymocytes, spleen cells and CD4+ T-cells share identical nucleotide sequences, and a high degree of homology with the corresponding nonlymphoid rat and human VIP-R1 sequences. The expression of VIP-R1 in thymocytes and peripheral lymphocytes, and especially in the CD4+ T-cell subset supports the idea that VIP produced or released locally in the lymphoid microenvironment could directly affect cytokine production and proliferation of T-lymphocytes.
authors.affiliation: Department of Biological Sciences, Rutgers University, New York, NJ 07102, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Johnson
authors.forename: R J
authors.initials: RJ
authors.lastname: McCormack
authors.forename: M
authors.initials: M
authors.lastname: Delgado
authors.forename: C
authors.initials: C
authors.lastname: Martinez
authors.forename: D
authors.initials: D
authors.lastname: Ganea
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000502:physiology*; D005260:Female; D008168:Lung / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000276:immunology / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide / Q000235:genetics*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
chemical_list: D012333:RNA, Messenger; D018005:Receptors, Vasoactive Intestinal Peptide; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; C495507:Vipr1 protein, mouse