Identification of a novel cardiac-specific transcript critical for cardiac myocyte differentiation.
A novel cDNA, pCMF1, which is expressed exclusively and transiently in the myogenic cells of the differentiating chicken heart was isolated and characterized. The full-length cDNA of pCMF1 has one open reading frame encoding 1538 predicted amino acids. While computer analysis predicts the presence of specific structural motifs, the overall sequence of pCMF1 is unique. The pattern of pCMF1 gene expression during heart formation was determined by whole-mount in situ hybridization. pCMF1 is transiently expressed within the myogenic cells of the primitive heart tube from stages 9 to 18 and is not detected in the heart or any other tissue thereafter. A replication-deficient retrovirus was used to mediate pCMF1 antisense expression in cardiogenic mesoderm. These analyses determined that the presence of pCMF1 antisense sequences disrupted myosin heavy chain expression during cardiac mesoderm differentiation. pCMF1 antisense had no effect on myosin heavy chain expression in differentiated cardiac myocytes. These data suggest a potential function for pCMF1 during cardiac myogenesis.
Wei
Y
Y
Program for Cell Biology and Genetics, Cornell University Medical College, New York, New York 10021, USA.
Bader
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D
Litvin
J
J
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D030161:Avian Proteins; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics*; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D017433:Protein Structure, Secondary; D015347:RNA Probes; D012190:Retroviridae / Q000235:genetics; D014158:Transcription, Genetic
D016375:Antisense Elements (Genetics); D030161:Avian Proteins; D018076:DNA, Complementary; D009124:Muscle Proteins; D015347:RNA Probes; C101539:cardiac muscle factor 1 protein, chicken; D018995:Myosin Heavy Chains