Effect of intraruminal propionic acid infusion on metabolism of mesenteric- and portal-drained viscera in growing steers fed a forage diet: II. Ammonia, urea, amino acids, and peptides.
This experiment investigated the effect of intraruminal infusion of propionic acid on the net flux of nitrogenous compounds across the mesenteric- (MDV) and portal-(PDV) drained viscera of seven Friesian steers, average BW 127 kg (SEM 4.6), fed a grass-pellet diet. Each received by random allocation 0 (control), .5, or 1.0 mol of propionic acid/d for 7 d. Blood flow in mesenteric and portal veins was determined by downstream dilution of p-aminohippuric acid in order to determine net appearance rates across the gastrointestinal tissues. Net urea and ammonia flux was unaffected by propionic acid supply. Circulating plasma free amino acid concentrations were increased (P < .05) by propionic acid infusion (2,235, 2,428, and 2,427, error mean square [EMS] 44,370 microM, for control, .5, and 1.0 mol of propionic acid/d, respectively). Net amino acid flux rates were increased at the highest rate of propionic acid infusion across MDV and PDV (4.66, 3.69, and 6.11, EMS 2.98 mol/d for MDV [P < .05] and 2.98, 2.45, and 3.73, EMS 1.69 mol/d for PDV [P < .10] for control, .5, and 1.0 mol of propionic acid/d respectively). Positive venous-arterio concentration differences for peptide-bound amino acids (PBAA) across the MDV and PDV indicated net appearance across the gastrointestinal tissues, but this was not affected by propionic acid infusion. The data show that amino acid flux across postruminal tissues can be influenced by ruminal propionic acid supply and that this does not affect PBAA appearance.
Seal
C J
CJ
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, U.K.
Parker
D S
DS
D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000641:Ammonia / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008642:Mesenteric Veins / Q000502:physiology; D010455:Peptides / Q000097:blood / Q000378:metabolism*; D011169:Portal Vein / Q000502:physiology; D011422:Propionates / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D014508:Urea / Q000097:blood / Q000378:metabolism*; D014781:Viscera / Q000098:blood supply* / Q000378:metabolism
D000596:Amino Acids; D010455:Peptides; D011422:Propionates; D000641:Ammonia; D014508:Urea