title: Tyrosine hydroxylase expression within Balb/C and C57black/6 mouse locus coeruleus. II. Quantitative study of the enzyme level.
abstract: The tyrosine hydroxylase phenotype expression was further investigated in the perikarya and pericoerulean areas of the locus coeruleus of two pure inbred mouse strains, Balb/C and C57Black/6, which the topological organization and phenotypic plasticity of the enzyme-containing cell population were previously studied. The tyrosine hydroxylase level and the mean protein quantity provided by each cell were significantly higher within the spaces delimited by the enzyme containing perikarya in the C57Black/6 strain as compared to the Balb/C strain. Three days after RU24722 administration, tyrosine hydroxylase tissue concentration and quantity were significantly increased in both strains. Two strain-dependant mechanisms of this pharmacologically induced protein modulation were demonstrated: the mean tyrosine hydroxylase quantity provided by each cell was increased in the C57Black/6 strain whereas the increase was obviously explained by the subset of additional tyrosine hydroxylase expressing cells previously reported in Balb/C strain. A comparable volume of pericoerulean immunolabeled neuropile, which contains a similar tyrosine hydroxylase level, was measured between the two strains. Within this tissue compartment, an undissociated RU24722 responsiveness was observed between the two strains: a significant increase in the protein level was measured principally resulting from a significant increase in the volume. These results revealed a strain-dependent difference in the response to the RU24722 treatment which may result from a genetic separation of two kinds of tyrosine hydroxylase phenotypic regulations within the perikarya area of the locus coeruleus; whereas the surrounding neuropile seemed to have a different mechanism of the phenotypic protein expression and modulation.
authors.affiliation: Laboratoire de Neuropharmacologie Moléculaire, CNRS-UCBL UMR 5542, Faculté de Médecine, Lyon, France.
authors.forename: N
authors.initials: N
authors.lastname: Ginovart
authors.forename: D
authors.initials: D
authors.lastname: Marcel
authors.forename: L
authors.initials: L
authors.lastname: Bezin
authors.forename: C
authors.initials: C
authors.lastname: Gagne
authors.forename: J F
authors.initials: JF
authors.lastname: Pujol
authors.forename: D
authors.initials: D
authors.lastname: Weissmann
mesh_terms: D000818:Animals; D008125:Locus Coeruleus / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D010641:Phenotype; D013045:Species Specificity; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000235:genetics
chemical_list: D014446:Tyrosine 3-Monooxygenase