title: Chemical modification of the arginyl residue in silk fibroin: 2. Reaction of 1,2-cyclohexanedione in aqueous alkaline medium.
abstract: The arginyl residue of solubilized silk fibroin was chemically modified with 1,2-cyclohexanedione in aqueous alkaline medium to form a stable imidazolidinone ring, and its positive charge was masked. CD spectra of the modified silk fibroin in aqueous solution showed an increase in the fraction of random coil conformation. The increase may be caused by the exposure to alkaline medium in the modification reaction. FT-IR and CD spectra of the silk fibroin films before and after the modification indicated that the conformational change in the modified silk fibroin in the solid state did not occur by the modification of its arginyl residue with 1,2-cyclohexanedione. The chemical stability of the modified silk fibroin film was investigated in vitro with phosphate-buffered saline solution. The modified arginyl residue in the film was stable in the phosphate-buffered solution.
authors.affiliation: National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Gotoh
authors.forename: M
authors.initials: M
authors.lastname: Tsukada
authors.forename: N
authors.initials: N
authors.lastname: Minoura
mesh_terms: D000818:Animals; D012831:Bombyx; D002942:Circular Dichroism; D003512:Cyclohexanones / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D008968:Molecular Conformation; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D003512:Cyclohexanones; C018031:1,2-cyclohexanedione; D005349:Fibroins