title: Presence of ED-A containing fibronectin in human articular cartilage from patients with osteoarthritis and rheumatoid arthritis.
abstract: OBJECTIVE
  To determine whether normal (fetal and adult) and osteoarthritic (OA) and rheumatoid arthritic (RA) cartilage express a specific isoform of fibronectin, the extra domain A (ED-A) containing fibronectin (EDA+Fn).
  
  
  METHODS
  Presence of fibronectin (EDA+Fn and native molecule) in cartilage matrix was studied using immunohistochemical assays with specific monoclonal antibodies. Fibronectins were identified by Western blots, in synovial fluids (SF) and cartilage extracts.
  
  
  RESULTS
  EDA+Fn was either moderately present in the surface zone or undetectable in normal cartilage, while it was increased in OA cartilage surface. In one OA cartilage sample, EDA+Fn was localized in the matrix distant from the cartilage surface (patches of staining) and its presence was confirmed by immunoblotting. In RA cartilage EDA+Fn was present in the pericellular areas of the different layers. By Western blots, the presence of EDA+Fn was confirmed in OA SF (2/3) and RA SF (3/3) (with different patterns of fragmentation).
  
  
  CONCLUSIONS
  EDA+Fn generally accumulates in the surface zone of OA cartilage, where it may play a role in extracellular matrix remodelling. Its presence was more abundant in SF and in cartilage from patients with RA.
authors.affiliation: Department of Rheumatology, Hopital Henri-Mondor, Creteil, France.
authors.forename: X
authors.initials: X
authors.lastname: Chevalier
authors.forename: P
authors.initials: P
authors.lastname: Claudepierre
authors.forename: N
authors.initials: N
authors.lastname: Groult
authors.forename: L
authors.initials: L
authors.lastname: Zardi
authors.forename: W
authors.initials: W
authors.lastname: Hornebeck
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D001344:Autopsy; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000378:metabolism*; D005260:Female; D005333:Fetus; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000378:metabolism; D008297:Male; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007375:Interleukin-1; D010446:Peptide Fragments