title: Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group.
abstract: Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.
authors.affiliation: Stanford University, California, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Shafer
authors.forename: M A
authors.initials: MA
authors.lastname: Winters
authors.forename: D L
authors.initials: DL
authors.lastname: Mayers
authors.forename: A J
authors.initials: AJ
authors.lastname: Japour
authors.forename: D R
authors.initials: DR
authors.lastname: Kuritzkes
authors.forename: O S
authors.initials: OS
authors.lastname: Weislow
authors.forename: F
authors.initials: F
authors.lastname: White
authors.forename: A
authors.initials: A
authors.lastname: Erice
authors.forename: K J
authors.initials: KJ
authors.lastname: Sannerud
authors.forename: A
authors.initials: A
authors.lastname: Iversen
authors.forename: F
authors.initials: F
authors.lastname: Pena
authors.forename: D
authors.initials: D
authors.lastname: Dimitrov
authors.forename: L M
authors.initials: LM
authors.lastname: Frenkel
authors.forename: P S
authors.initials: PS
authors.lastname: Reichelderfer
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D011088:DNA Ligases; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D007753:Laboratories; D007963:Leukocytes, Mononuclear / Q000821:virology; D009154:Mutation; D010949:Plasma / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D003062:Codon; D017931:DNA Primers; D004279:DNA, Viral; D015215:Zidovudine; D011088:DNA Ligases