Strain-related differences in the ability of T lymphocytes to recognize proteins encoded by the golli-myelin basic protein gene.
Protein products of the golli-MBP gene complex, expressed in the nervous and lymphoid systems, contain sequences in common with sequences in 'classic' MBP, expressed exclusively in the nervous system. In this report, it was determined whether T cell lines (TCLs) specific for encephalitogenic epitopes of 'classic' MBP were able to recognize sequences within golli-MBP. TCLs derived from SJL mice specific for the immunodominant 83-102 sequence and the subdominant 19-27 sequence of 'classic' MBP recognized golli-MBP J37 and BG21, respectively. In contrast, TCLs derived from PL and B10.PL mice specific for the immunodominant 1-9 sequence of 'classic' MBP did not recognize this sequence within either J37 or BG21. These strain-related differences in the ability of golli-MBP proteins to stimulate 'classic' MBP-specific TCLs are discussed with respect to a possible influence on whether the course of EAE is relapsing (SJL) or monophasic (PL and B10.PL).
Pitchekian-Halabi
H
H
Reed Neurological Research Center, UCLA Dept. of Neurology 90024, USA.
Campagnoni
C W
CW
Skinner
E
E
Kampf
K
K
Campagnoni
A T
AT
Voskuhl
R R
RR
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D018604:Epitope Mapping; D005260:Female; D015870:Gene Expression / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics* / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D004676:Myelin Basic Protein; D010446:Peptide Fragments