Increased 3-nitrotyrosine in brains of Apo E-deficient mice.
Apolipoprotein E (Apo-E) is linked to the pathogenesis of Alzheimer's disease. Apo-E deficient mice have increased lipid peroxidation in plasma. In the present study we examined two markers of oxidative stress in brains of Apo-E deficient mice. The ratios of 2,3 and 2,5 dihydroxybenzoic acid (DHBA)/salicylate, an index of hydroxyl radical generation, were unchanged except for an increase in 2.5-DHBA/salicylate in the cerebellum. 3-Nitroxyrosine is a marker for nitration of proteins produced by peroxynitrite. Concentrations of 3-nitrotyrosine were significantly increased in the cerebral cortex, hippocampus, brainstem and cerebellum of Apo-E deficient mice. These results suggest the Apo-E may modulate oxidative stress produced by peroxynitrite.
Matthews
R T
RT
Neurochemistry Laboratory, Massachusetts General Hospital, Boston 02114, USA.
Beal
M F
MF
D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D015415:Biomarkers; D001923:Brain Chemistry / Q000502:physiology*; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D005841:Gentisates; D062385:Hydroxybenzoates / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D001057:Apolipoproteins E; D015415:Biomarkers; D016166:Free Radical Scavengers; D005609:Free Radicals; D005841:Gentisates; D062385:Hydroxybenzoates; D012459:Salicylates; C002744:3-nitrotyrosine; D014443:Tyrosine; C009135:2,3-dihydroxybenzoic acid; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid