Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast cells.
Histamine release from purified rat peritoneal mast cells (PMC) was examined and compared to that from a non-purified preparation (PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with compound 48/80, calcium ionophore A23187 and substance P. In contrast, IgE-dependent histamine release from PMC caused by antigen, anti-IgE and concanavalin A was very low compared to that of PEC. The reduced IgE-dependent histamine release from PMC, however, was recovered when PMC was reconstituted with non-mast cells (NMC) present in the peritoneal cavity. The effect was time-dependent and reached a plateau in 30 min. NMC from both sensitized and non-sensitized rats recovered the reduced histamine release from PMC dose-dependently. The potentiating effect of NMC was observed even in the presence of excess amount of phosphatidylserine. Supernatants of NMC and a mixture of PMC and NMC incubated for 1 hr at 37 degrees C, however, failed to potentiate the histamine release. These results demonstrate that IgE-dependent histamine release from rat peritoneal mast cells is upregulated by other cells present in the peritoneal cavity, and that the mechanism involved is distinct from that of phosphatidylserine.
Inagaki
N
N
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Kawasaki
H
H
Nagai
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D002021:Buffers; D002469:Cell Separation; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D007073:Immunoglobulin E / Q000502:physiology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D002021:Buffers; D007202:Indicators and Reagents; D007073:Immunoglobulin E