Supravital staining of eosinophils.
Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.
Stankiewicz
M
M
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. stankiewiczm@agresearch.cri.nz
Jonas
W
W
Hadas
E
E
Cabaj
W
W
Douch
P G
PG
D000165:Acridine Orange; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure*; D004804:Eosinophils / Q000737:chemistry / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small; D007958:Leukocyte Count; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods
D000165:Acridine Orange