Experimental immunization of hamsters with an EDTA extract of Leptospira interrogans, serovar icterohaemorrhagiae.
The EDTA extract was prepared from the Leptospira interrogans serovar icterohaemorrhagiae. When inoculated subcutaneously in hamsters it conferred protection from challenge with virulent leptospires with a low dose (10 micrograms/ml) and low agglutinating antibody (40). The protection of animals obtained by the EDTA extract opens up the perspective of its use as a component of a vaccine for the control of leptospirosis.
de Queiroz-Leite
L T
LT
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil.
Resende
M
M
Ramos-Vieira M das
N
N
Cota-Koury
M
M
D000373:Agglutinins / Q000096:biosynthesis / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D004306:Dose-Response Relationship, Immunologic; D004492:Edetic Acid; D005069:Evaluation Studies as Topic; D007279:Injections, Subcutaneous; D007668:Kidney / Q000382:microbiology; D007921:Leptospira interrogans / Q000737:chemistry / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D008647:Mesocricetus; D012997:Solvents; D014611:Vaccination
D000373:Agglutinins; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D012997:Solvents; D004492:Edetic Acid