Induction of mcl1/EAT, Bcl-2 related gene, by retinoic acid or heat shock in the human embryonal carcinoma cells, NCR-G3.
NCR-G3 cells were established from a testicular embryonal carcinoma and were differentiated into multi-lineages including trophectoderm cells by exposure to retinoic acid. The differentiated cells began to produce human chorionic gonadotropin (hCG), a trophectoderm-specific hormone, which was regulated at the mRNA level. As we assumed that genes responsible for differentiation were differentially expressed at the early stage of retinoic acid-induced differentiation, we prepared a cDNA library from retinoic acid-treated NCR-G3 cells. This cDNA library was then screened for genes whose expression was induced during the differentiation of these cells. From about 5 x 10(4) clones screened, three independent sequences were isolated. Sequencing analysis revealed that clone 1002 codes for mcl1/EAT, which has a Bcl-2 homology domain. The expression of mcl1/EAT, the Bcl-2 related gene, was increased at an early stage of the retinoic acid-induced differentiation and preceded the up-regulation of cytokeratin and hCG genes after ratinoic acid treatment. Furthermore, mcl1/EAT was also up-regulated by heat shock, which has recently been shown to induce the cells to differentiate.
Umezawa
A
A
Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
Maruyama
T
T
Inazawa
J
J
Imai
S
S
Takano
T
T
Hata
J
J
D015415:Biomarkers; D002273:Carcinogens / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D006063:Chorionic Gonadotropin; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000302:isolation & purification; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation / Q000187:drug effects; D019254:Genes, bcl-2 / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D008297:Male; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010703:Phorbol Esters / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D013736:Testicular Neoplasms; D014212:Tretinoin / Q000494:pharmacology
D015415:Biomarkers; D002273:Carcinogens; D006063:Chorionic Gonadotropin; D018076:DNA, Complementary; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D010703:Phorbol Esters; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D014212:Tretinoin