title: Impaired IgG-dependent anaphylaxis and Arthus reaction in Fc gamma RIII (CD16) deficient mice.
abstract: The family of receptors for IgG (Fc gamma R) plays an essential role in antibody-mediated effector functions of the immune system. However, the specific contribution of each of the Fc gamma R classes to in vivo immune reactions is still unclear. Here, we demonstrate that mice deficient for the ligand-binding alpha chain of Fc gamma RIII lack NK cell-mediated antibody-dependent cytotoxicity and phagocytosis of IgG1-coated particles by macrophages. Strikingly, these mice lack IgG-mediated mast cell degranulation, are resistant to IgG-dependent passive cutaneous anaphylaxis, and exhibit an impaired Arthus reaction. These results indicate a prominent role for Fc gamma RIII in inflammatory and anaphylactic responses, making this receptor a potential target in immunotherapy.
authors.affiliation: Department of Immunology, University Hospital Utrecht, The Netherlands.
authors.forename: W L
authors.initials: WL
authors.lastname: Hazenbos
authors.forename: J E
authors.initials: JE
authors.lastname: Gessner
authors.forename: F M
authors.initials: FM
authors.lastname: Hofhuis
authors.forename: H
authors.initials: H
authors.lastname: Kuipers
authors.forename: D
authors.initials: D
authors.lastname: Meyer
authors.forename: I A
authors.initials: IA
authors.lastname: Heijnen
authors.forename: R E
authors.initials: RE
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Sandor
authors.forename: P J
authors.initials: PJ
authors.lastname: Capel
authors.forename: M
authors.initials: M
authors.lastname: Daëron
authors.forename: J G
authors.initials: JG
authors.lastname: van de Winkel
authors.forename: J S
authors.initials: JS
authors.lastname: Verbeek
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001183:Arthus Reaction / Q000276:immunology*; D015550:Cell Degranulation / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D017452:Receptors, IgG / Q000172:deficiency* / Q000235:genetics*; D012756:Sheep / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D017452:Receptors, IgG