title: The gene for X-linked hypophosphataemic rickets maps to a 200-300kb region in Xp22.1, and is located on a single YAC containing a putative vitamin D response element (VDRE).
abstract: The location of the HYP gene, which determines X-linked hypophosphataemic rickets, has been refined considerably by linkage analysis, and three new microsatellite primers isolated, Cap32 (DXS7473), Cap29 (DXS7474) and 7v2 (DXS7475). The locations of four other markers have also been determined (DXS1226, AFMa176zb1, AFMa152wc5, and AFM346azc1). Markers Cap29 and Cap32 are the closest distal markers to the gene with zetamax=11.93, thetamax= 0.018 and zetamax=12.03, thetamax = 0.015 respectively. Both Cap29 and Cap32 are proximal to DXS365 and AFMa176zb1, as deduced by screening non-chimaeric yeast artificial chromosomes (YACs) from a contig spanning the HYP gene. A single crossover places AFMa176zbl distal to the disease gene. There are no recombinations between 7v2 and HYP (zetamax=12.9, thetamax=0.0), or between 7v2 and adjacent markers Cap32, Cap29, AFMa176zb1, DXS1683 and DXS365. However screening of YAC clones encompassing the HYP gene and also P1 clones localises 7v2 distal to Cap29 and Cap32, and proximal to DXS443. Marker DXS1226 is placed outside the region containing the gene, and is located proximal to DXS274 as confirmed by a crossover for this marker and DXS41 against HYP and its presence on YAC 83B05. Genetic mapping of CEPH pedigrees, and screening of YACs places AFMa152wc5 and AFMa346zcl between DXS1683 and DXS1052. The following gene marker map presents the best order for the HYP region: Xptel-DXS43-DXS999-DXS443-(DXS365/DXS74  75/AFMa176zb1)-(DXS7474/DXS7473)-HYP- DXS1683-(AFMa152wc5/AFMa346zc1)-DXS1052-DXS 274 -(DXS41/DXS1226)-Xcen. The distance between the cluster of distal flanking markers Cap29 (DXS7474), Cap32 (DXS7473), and DXS1683 is approximately 300 kb, as deduced from physical map data from a YAC contig spanning the gene. Thus the gene for HYP is contained within a single YAC (900AO472). Of further interest, is the location of a putative vitamin D response element (VDRE) on this YAC.
authors.affiliation: University College London, Department of Medicine, UK.
authors.forename: P S
authors.initials: PS
authors.lastname: Rowe
authors.forename: J N
authors.initials: JN
authors.lastname: Goulding
authors.forename: F
authors.initials: F
authors.lastname: Francis
authors.forename: C
authors.initials: C
authors.lastname: Oudet
authors.forename: M J
authors.initials: MJ
authors.lastname: Econs
authors.forename: A
authors.initials: A
authors.lastname: Hanauer
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: A P
authors.initials: AP
authors.lastname: Read
authors.forename: R C
authors.initials: RC
authors.lastname: Mountford
authors.forename: T
authors.initials: T
authors.lastname: Summerfield
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: W
authors.initials: W
authors.lastname: Fraser
authors.forename: M K
authors.initials: MK
authors.lastname: Drezner
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
authors.forename: J L
authors.initials: JL
authors.lastname: O'Riordan
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D004276:DNA, Satellite / Q000032:analysis; D008040:Genetic Linkage; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000235:genetics*; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D018167:Receptors, Calcitriol / Q000235:genetics*; D014960:X Chromosome
chemical_list: D004276:DNA, Satellite; D018167:Receptors, Calcitriol