Plasma glutathione peroxidase deficiency caused by renal dysfunction.
Glutathione peroxidase (GPx) activity was determined in the plasma of 118 healthy persons, 18 nondialyzed patients with chronic renal failure (CRF), 20 patients on maintenance hemodialysis (HD), and 58 patients on continuous ambulatory peritoneal dialysis (CAPD). Serum creatinine levels in the nondialyzed CRF patients revealed a highly significant negative correlation (r = -0.71, p < 0.001) with plasma GPx activity. Immunoblot analysis revealed that the plasma (extracellular) GPx protein was reduced or undetectable in patients with low plasma GPx activity. Plasma GPx activities in the HD and CAPD patients were reduced to 44 and 23% (female), and to 70 and 45% (male) of the sex-matched control values, respectively. In contrast, erythrocyte (cellular) GPx activity was not decreased in the nondialyzed CRF patients and the dialyzed patients. Plasma selenium concentrations were within the normal range in these patient groups. These results indicate that the plasma GPx activity largely depends on renal function.
Yoshimura
S
S
Department of Molecular Life Science 'Cell Biology', Tokai University School of Medicine, Kanagawa, Japan.
Suemizu
H
H
Nomoto
Y
Y
Sakai
H
H
Katsuoka
Y
Y
Kawamura
N
N
Moriuchi
T
T
D000368:Aged; D004912:Erythrocytes / Q000201:enzymology; D005110:Extracellular Space / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000172:deficiency*; D006801:Humans; D015151:Immunoblotting; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D006435:Renal Dialysis; D012643:Selenium / Q000097:blood
D005979:Glutathione Peroxidase; D012643:Selenium