Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: an immunohistochemical study in the rat.
An antibody which recognizes specifically a metabotropic glutamate receptor, mGluR7, was produced by using a trpE fusion protein containing a C-terminal sequence of rat mGluR7. Neuropil in laminae I and II of the dorsal horn of the rat, as well as many neuronal cell bodies in the dorsal root ganglion, showed mGluR7-like immunoreactivity; the immunoreactivity in neuropil was seen in axon terminals, which were filled with round synaptic vesicles and constituted axodendritic and axosomatic asymmetric synapses. The mGluR7-like immunoreactivity in laminae I and II in the dorsal horn was reduced after dorsal rhizotomy. The results indicate that some axon terminals of the primary afferent fibers to laminae I and II of the dorsal horn are provided with mGluR7.
Ohishi
H
H
Department of Morphological Brain Science, Kyoto University, Japan.
Nomura
S
S
Ding
Y Q
YQ
Shigemoto
R
R
Wada
E
E
Kinoshita
A
A
Li
J L
JL
Neki
A
A
Nakanishi
S
S
Mizuno
N
N
D000818:Animals; D000918:Antibody Specificity; D005727:Ganglia, Spinal / Q000737:chemistry / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009475:Neurons, Afferent / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis* / Q000276:immunology; D017661:Receptors, Presynaptic / Q000032:analysis* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013116:Spinal Cord / Q000737:chemistry / Q000648:ultrastructure
D018094:Receptors, Metabotropic Glutamate; D017661:Receptors, Presynaptic; D011993:Recombinant Fusion Proteins