Role of calcium in photodynamically induced cell damage of human fibroblasts.
Photodynamically induced changes in the cytoplasmic free calcium concentration ([Ca2+]i) and its role in cell damage were investigated in human skin fibroblasts using confocal laser microscopy. Fluorescence and absorbance spectrophotometry measurements indicate that the photosensitizer aluminum phthalocyanine tetrasulfonate (AIPcS4) binds to the plasma membrane and only after irradiation is able to enter the cells, causing massive morphologic alterations. Upon irradiation of sensitizer-treated cells, the increase in [Ca2+]i is related to the amount of light and extracellular [Ca2+]e. The increase in [Ca2+]i was substantially reduced in the absence of [Ca2+]a. Cell damage or death after photodynamic treatment was prevented and shifted toward higher fluence by increasing [Ca2+]i at high [Ca2+]e and was greater at low [Ca2+]e. Application of Ca2+ channel blockers, such as Co2+, Cd2+ or verapamil, could not prevent the increase of [Ca2+]i. Our results indicate that activation of the photosensitizer, AIPcS4, causes an influx of Ca2+, which protects cells from, photodamage. At low [Ca2+]e and high fluence values, release of Ca2+ from internal stores probably as a protective measure occurs in order to increase the [Ca2+]i.
Hubmer
A
A
Institute of Physics and Biophysics, University of Salzburg, Austria.
Hermann
A
A
Uberriegler
K
K
Krammer
B
B
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005347:Fibroblasts; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles / Q000493:pharmacokinetics / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D009942:Organometallic Compounds / Q000493:pharmacokinetics / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*
D005456:Fluorescent Dyes; D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C048577:aluminum tetrasulfophthalocyanine; D002118:Calcium