title: Acyl CoA and lipid synthesis from ketone bodies by the extramitochondrial fraction of hepatoma tissue.
abstract: The compartmentation of the pathway for the synthesis of lipids from ketone bodies was examined in a cell free, post-mitochondrial supernatant fraction of malignant Morris hepatoma 7777 tissue. A fortified supernatant system effectively incorporated radioactive D(-)-3-hydroxybutyrate and acetoacetate into cholesterol and fatty acids, and both ketone bodies were directly converted to their CoA thioesters. Furthermore, a microsome-free (100,000 x g) cytosolic fraction was also able to acylate 3-hydroxybutyrate to 3-hydroxybutyryl CoA. No previous identification of this enzyme activity has been described. These results which characterize a distinct extramitochondrial pathway for conversion of 3-hydroxybutyrate as well as acetoacetate into lipids also suggest the possibility of a previously undetected enzymatic activity for utilization of this ketone body.
authors.affiliation: Department of Medicine, University of Wisconsin, Madison 53706, USA.
authors.forename: L
authors.initials: L
authors.lastname: Hildebrandt
authors.forename: T
authors.initials: T
authors.lastname: Spennetta
authors.forename: R
authors.initials: R
authors.lastname: Ackerman
authors.forename: C
authors.initials: C
authors.lastname: Elson
authors.forename: E
authors.initials: E
authors.lastname: Shrago
mesh_terms: D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002951:Citrates / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism*; D007700:Kinetics; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008928:Mitochondria; D011865:Radioisotope Dilution Technique; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000090:Acetoacetates; D000214:Acyl Coenzyme A; D002250:Carbon Radioisotopes; D002951:Citrates; D006885:Hydroxybutyrates; D007657:Ketone Bodies; C016635:acetoacetic acid; D020155:3-Hydroxybutyric Acid