FAD-glycerophosphate dehydrogenase activity in lymphocytes of type-2 diabetic patients and their relatives.
The activities of FAD-linked glycerophosphate dehydrogenase (m-GDH), glutamate dehydrogenase (GlDH), glutamate-pyruvate transaminase (GPT) and glutamate-oxalacetate transaminase (GOT) were measured in purified populations of CD3+ lymphocytes from 55 control subjects, 62 type-2 diabetics and 50 non-diabetic relatives of the latter patients. The activity of m-GDH was measured by both a radioisotopic procedure and colourimetric technique. As judged from these measurements and relative to the paired value for GlDH, the incidence of abnormally low m-GDH activity was significantly higher in type-2 diabetics than in control subjects. Moreover, the paired ratio in reaction velocity between the colourimetric and radioisotopic assay of m-GDH was abnormally high in patients with low m-GDH activity. Low m-GDH activity often coincided with increased GPT activity in plasma or high GPT/GOT ratio in lymphocytes. No obvious clustering of these anomalies was found in relatives of diabetic patients. These findings suggest that an inherited or acquired genomic defect of m-GDH in lymphocytes, and possibly in pancreatic B-cells, may participate to the pathogenesis of non-insulin-dependent diabetes mellitus.
Vidal
J
J
Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University, Belgium.
Rasschaert
J
J
Sener
A
A
Gomis
R
R
Malaisse
W J
WJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000410:Alanine Transaminase / Q000097:blood; D015703:Antigens, CD; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D002096:C-Peptide / Q000097:blood; D017252:CD3 Complex; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology* / Q000235:genetics*; D005190:Family; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D012016:Reference Values; D013601:T-Lymphocytes / Q000201:enzymology*; D014280:Triglycerides / Q000097:blood
D000410:Alanine Transaminase; D015703:Antigens, CD; D001219:Aspartate Aminotransferases; D001786:Blood Glucose; D002096:C-Peptide; D017252:CD3 Complex; D002784:Cholesterol; D006442:Glycated Hemoglobin; D005993:Glycerolphosphate Dehydrogenase; D014280:Triglycerides; C098831:glycerol-3-phosphate oxidase