Molecular cloning of, and phylogenetic analysis of, an actin in Naegleria fowleri.
We cloned and sequenced an intronless actin gene from the amoebo-flagellate Naegleria fowleri, LEE strain, an opportunistic pathogen of man. Codon usage and third-position-codon nucleotide frequency were significantly different from Acanthamoeba, another amoeba genus which also includes opportunistic pathogens of man. Between the two amoebae, actin peptide sequences were 92.8% similar, while nucleotide sequences were only 70% similar. A phylogenetic reconstruction of actin amino acid sequences, using a distance method, placed Naegleria in a cluster with Plasmodium and Entamoeba.
Gorospe
S
S
Department of Zoology, Michigan State University, East Lansing 48824-1115, USA.
Band
R N
RN
Kopachik
W J
WJ
D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016848:Naegleria fowleri / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny
D000199:Actins