title: A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins.
abstract: Following the analysis of transposon Tn5432-induced mutants of Corynebacterium glutamicum ATCC 13032, a gene encoding a protein with a biotin-binding motif was cloned. The DNA sequence of this gene revealed an open reading frame encoding 591 amino acids with a calculated mol. mass of 63.4 kDa. The protein is composed of two domains, an N-terminal biotin carboxylase and a C-terminal biotin-carboxyl-carrier protein, that are highly similar to corresponding subunits from prokaryotic and eukaryotic biotin enzymes. Over 70% identity was found to a protein from Mycobacterium leprae proposed to be part of an acyl-CoA carboxylase. Since it was not possible to inactivate the C. glutamicum gene, the gene most likely encodes a subunit of the essential acetyl-CoA carboxylase, which catalyzes the committed step in fatty acid synthesis.
authors.affiliation: Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Jäger
authors.forename: P G
authors.initials: PG
authors.lastname: Peters-Wendisch
authors.forename: J
authors.initials: J
authors.lastname: Kalinowski
authors.forename: A
authors.initials: A
authors.lastname: Pühler
mesh_terms: D000595:Amino Acid Sequence; D019731:Carbon-Nitrogen Ligases; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; C017071:biotin-binding proteins; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019791:biotin carboxylase