Generation of a rat bronchiolar epithelial cell cDNA library: isolation of a proline rich protein highly enriched in bronchiolar epithelial cells.
As a tool for the identification of novel gene products expressed in rat bronchiolar epithelial (BE) cells we generated a cDNA library prepared from BE cells, enriched to contain > 40% Clara cells. Using a simple differential screening strategy we isolated and partially characterized 47 clones, 29 of which corresponded to gene products known to be expressed within the bronchiolar epithelium. A further 15 clones contained sequences which were not present in the EMBL database and therefore represented potential novel BE cell enriched clones. One of these clones, CC-4, was characterized further and shown by northern blotting to be highly enriched in the BE cell population. The deduced protein product of CC-4 is a proline rich protein of 29.5 kd. These studies suggest that this cDNA library may be a useful tool for the identification of novel lung restricted gene products.
Bingle
C D
CD
Royal London School of Medicine and Dentistry, United Kingdom. C.D.Bingle@mds.qmw.ac.uk
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001980:Bronchi / Q000737:chemistry* / Q000166:cytology; D002469:Cell Separation; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011392:Proline / Q000032:analysis; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D011506:Proteins; D011392:Proline