Immunochemical characterization of antibody-coated nanoparticles.
A new method using surface plasmon resonance (SPR) through the BIAcore was used to demonstrate the specific interaction between an anti-CD4 monoclonal antibody (IOT4a), adsorbed on poly(methylidene malonate 2.1.2) (PMM 2.1.2) nanoparticles, and the CD4 molecule. The results obtained were compared with the interaction of the same immunonanoparticles with rabbit anti-mouse Fc antibodies. The molar ratio (Fc)/(Fab) was 1, suggesting that the same number of epitopes on the Fc and the Fab fragments were accessible after IOT4a adsorption onto nanoparticles. Comparing the observed association rates of free antibody and antibody adsorbed on nanoparticles, the number of molecules of IOT4a antibody on PMM 2.1.2 nanoparticles was estimated as between 2.6 and 3 per nanoparticle. The properties of the antibody-coated nanoparticles are compatible with their use as antibody-targeted pharmacophores.
Velge-Roussel
F
F
CJF INSERM 93-09, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, Tours (France).
Breton
P
P
Guillon
X
X
Lescure
F
F
Bru
N
N
Bout
D
D
Hoebeke
J
J
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D015704:CD4 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004337:Drug Carriers; D004339:Drug Compounding; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007700:Kinetics; D008314:Malonates / Q000378:metabolism*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011095:Polyethylenes / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D004337:Drug Carriers; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D008314:Malonates; D011095:Polyethylenes; D011994:Recombinant Proteins; C091414:poly(methylidene malonate) 2.1.2; C076613:recombinant soluble CD4