Ascorbic acid preferentially enhances type I and III collagen gene transcription in human skin fibroblasts.
Ascorbic acid is a potent stimulator for type I and III collagen expression in human skin fibroblasts; stimulation of type I and III collagen synthesis and their mRNA levels by ascorbic acid has been reported previously. Nuclear run-on experiments demonstrated that ascorbic acid enhanced the transcription of type I and III collagen genes 4- and 3.4-fold respectively, whereas transcription of type IV collagen was slightly stimulated (1.7-fold). The results suggest that ascorbic acid preferentially enhanced type I and III collagen transcription.
Tajima
S
S
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Pinnell
S R
SR
D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects* / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D003094:Collagen; D001205:Ascorbic Acid