Stoichiometry of neurally induced VIP release, NO formation, and relaxation in rabbit and rat gastric muscle.
Vasoactive intestinal peptide (VIP) release, nitric oxide (NO) formation, and relaxation induced by nerve stimulation were examined in rabbit and rat gastric muscle. VIP stimulated NO formation in muscle strips, whereas NO stimulated VIP release. Nerve stimulation (0.025-16 Hz or 2-940 pulses) elicited frequency-dependent stimulation of VIP release, NO formation, and relaxation, all significant at two to three pulses. NG-nitro-L-arginine (L-NMA) abolished NO formation, abolished VIP release and relaxation at low frequencies, and partly inhibited them at higher frequencies. Oxyhemoglobin (oxy-Hb) inhibited VIP release and relaxation by 80% at low frequencies and 20-30% at higher frequencies. VIP-(10-28) abolished NO formation and relaxation at lower frequencies and partly inhibited them at higher frequencies; in contrast, VIP-(10-28) augmented VIP release in both species. The pattern of inhibition was similar in both species. Inhibition of maximal NO formation by VIP-(10-28) (82% in rabbit; 48% in rat) implied that a major component of NO is formed in muscle cells by the action of VIP. Thus 1) inhibition of relaxation by L-NNA reflects suppression of NO and VIP release from nerve terminals and NO formation in muscle cells, 2) inhibition by VIP-(10-28) partly reflects suppression of NO formation in muscle cells, and 3) inhibition by oxy-Hb reflects neutralization of extracellular NO and suppression of VIP release. The study demonstrates the dual origin of NO from nerves and muscle and its interplay with VIP in regulating relaxation.
Jin
J G
JG
Department of Medicine and Physiology, Medical College of Virginia, Richmond 23298-0711, USA.
Murthy
K S
KS
Grider
J R
JR
Makhlouf
G M
GM
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008433:Mathematics; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology*; D009424:Nervous System Physiological Phenomena; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D010108:Oxyhemoglobins / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013270:Stomach / Q000187:drug effects / Q000294:innervation* / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000494:pharmacology
D010108:Oxyhemoglobins; D010446:Peptide Fragments; D009569:Nitric Oxide; D014660:Vasoactive Intestinal Peptide; C047945:vasoactive intestinal peptide (10-28)