title: New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.
abstract: Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.
authors.affiliation: Institute of Medical Virology, JWG-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: J U
authors.initials: JU
authors.lastname: Vogel
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: A
authors.initials: A
authors.lastname: Lux
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: A J
authors.initials: AJ
authors.lastname: Driesel
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis / Q000821:virology; D001483:Base Sequence; D002493:Central Nervous System Diseases / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral