title: Amino acid sequence determination of human S100A12 (P6, calgranulin C, CGRP, CAAF1) by tandem mass spectrometry.
abstract: S100A12 has been isolated from human neutrophils. The molecular weight and the amino acid sequence of S100A12 was determined by electrospray-mass spectrometry, tandem mass spectrometry and Edman microsequence analysis. Interestingly, a sequence comparison of S100A12 with all known human S100 proteins revealed that S100A12 is the most divergent of the S100 proteins.
authors.affiliation: Department of Pediatrics, University of Zurich, Switzerland.
authors.forename: E C
authors.initials: EC
authors.lastname: Ilg
authors.forename: H
authors.initials: H
authors.lastname: Troxler
authors.forename: D M
authors.initials: DM
authors.lastname: Bürgisser
authors.forename: T
authors.initials: T
authors.lastname: Kuster
authors.forename: M
authors.initials: M
authors.lastname: Markert
authors.forename: F
authors.initials: F
authors.lastname: Guignard
authors.forename: P
authors.initials: P
authors.lastname: Hunziker
authors.forename: N
authors.initials: N
authors.lastname: Birchler
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D009418:S100 Proteins; D000067616:S100A12 Protein; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human; D015740:Calcitonin Gene-Related Peptide