[An in-vitro leukocytolysis method for the diagnosis of a drug allergy].
The authors analyze published data on various modifications of the specific leukocyte lysis test (SLLT) and their own findings on in vitro osmotic leukocytolysis caused by low-molecular nonelectrolytes (urea, glycerol, and glucose), and present the estimation of osmotic pressure of these agents' solutions in equimolar concentrations. In contrast to the specific effect of allergens, an osmotic component of nonspecific leukocyte lysis is considered. A universal approach to the choice of working doses of drug in SLLT is proposed: equimolar solutions of the allergens (chemical and medicinal compounds). The necessity of further theoretical and practical research of SLLT is validated.
Vavilov
M P
MP
Kiselev
A S
AS
Nazarova
E K
EK
D000485:Allergens; D004305:Dose-Response Relationship, Drug; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007962:Leukocytes / Q000187:drug effects*; D008970:Molecular Weight; D009997:Osmotic Pressure; D012996:Solutions
D000485:Allergens; D012996:Solutions
Metod leĭkotsitoliza in vitro dlia diagnostiki lekarstvennoĭ allergii.