AMP is a component of the low molecular weight mitogenic activity present in human pituitary tumours.
The mitogenic activity of extracts of human non-functional pituitary tumours has been studied. Previously we have reported that the tumour extracts could be resolved into both high (> 13,000) and low (< 3,000) molecular weight fractions using Sephadex G-50 chromatography. Mitogenic activity was assayed by looking at trichloroacetic acid precipitable 3H-thymidine incorporation into GH3 cells. We have now purified the major component of the low molecular weight mitogenic fraction using reversed phase HPLC: the material was identified and found to be 5'-adenosine monophosphate (5'-AMP) using electron spray mass spectrometry. The mitogenic activity of 5'-AMP and the purified tumour extract was confirmed as both produced an increase in GH3 cell number after 4 days of treatment. In conclusion our results show that the major component of the low molecular weight mitogenic activity in human non-functional pituitary tumour extracts is 5'-AMP.
Lewis
M D
MD
Department of Medicine, University of Wales College of Medicine Health Park, Cardiff, UK.
Webster
J
J
Ham
J
J
Davies
J S
JS
Scanlon
M F
MF
D000249:Adenosine Monophosphate / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008934:Mitogens / Q000378:metabolism*; D008970:Molecular Weight; D010911:Pituitary Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology
D008934:Mitogens; D014020:Tissue Extracts; D000249:Adenosine Monophosphate; D013936:Thymidine