title: Two new leptospiral serovars in the Hebdomadis serogroup isolated from Zimbabwe cattle.
abstract: Four strains belonging to the genus Leptospira serogroup Hebdomadis were isolated from Zimbabwe cattle at slaughter. These isolates were subjected to cross-agglutinin absorption tests and to restriction fragment length polymorphism and pulsed-field gel electrophoresis analyses of their genomic DNAs. One of these strains represents a new serovar, for which the name mhou is proposed; strain SBF 40 is the reference strain of this serovar. The other three strains belong to a second new serovar, for which the name marondera is proposed; the reference strain of this serovar is strain SBF 5. The three strains of serovar marondera could be differentiated by their restriction fragment polymorphism and pulsed-field gel electrophoretic patterns.
authors.affiliation: Department of Biological Sciences, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe. feresu@zimbix.uz.zw
authors.forename: S B
authors.initials: SB
authors.lastname: Feresu
authors.forename: H
authors.initials: H
authors.lastname: Korver
authors.forename: N
authors.initials: N
authors.lastname: Riquelme
authors.forename: G
authors.initials: G
authors.lastname: Baranton
authors.forename: C A
authors.initials: CA
authors.lastname: Bolin
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D007919:Leptospira / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D015030:Zimbabwe
chemical_list: D004269:DNA, Bacterial