Investigation of phagocyte-inducible factor in tumor-implanted mice.
The number of spleen Mac-1-positive cells was markedly increased in methylcholanthrene-induced Meth-A fibrosarcoma (Meth-A)-implanted mice. When the sera of Meth-A-implanted mice were added to normal bone marrow cells, granulocyte and macrophage colony stimulating factor (GM-CSF)-dependent growth of bone marrow cells was significantly enhanced. Analysis of the Meth-A-implanted mice sera showed that the levels of serum transferrin were markedly elevated. When the sera of Meth-A-implanted mice were pretreated with anti-mouse transferrin antibody, the enhancement of GM-CSF-dependent cell growth was almost abolished. Cell growth was also stimulated by the addition of transferrin which also caused an increase in the number of Mac-1-positive cells. Analysis of the effect of transferrin on bone marrow cells showed that the response of the cells to GM-CSF was significantly increased by preincubation with transferrin.
Watanabe
T
T
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
Okada
A
A
Mikami
T
T
Matsumoto
T
T
Suzuki
M
M
D000818:Animals; D000906:Antibodies / Q000097:blood; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D005354:Fibrosarcoma / Q000097:blood* / Q000139:chemically induced / Q000473:pathology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000819:agonists*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000473:pathology; D014168:Transferrin / Q000276:immunology / Q000494:pharmacology*
D000906:Antibodies; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D014168:Transferrin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor