title: A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia.
abstract: A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.
authors.affiliation: School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Nathan Brisbane, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Wynter
authors.forename: B K
authors.initials: BK
authors.lastname: Patel
authors.forename: P
authors.initials: P
authors.lastname: Bain
authors.forename: J
authors.initials: J
authors.lastname: de Jersey
authors.forename: S
authors.initials: S
authors.lastname: Hamilton
authors.forename: P A
authors.initials: PA
authors.lastname: Inkerman
mesh_terms: D001315:Australia; D001421:Bacteria, Anaerobic / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D050260:Carbohydrate Metabolism; D003910:Dextranase / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D005618:Fresh Water / Q000382:microbiology; D005798:Genes, Bacterial; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D006207:Half-Life; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003910:Dextranase