title: Conserved secondary structure in the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2) and the fatty acid synthase acyl carrier protein from Escherichia coli.
abstract: The acyl carrier protein (ACP) of Streptomyces coelicolor A3(2) functions as a molecular chaperone during the biosynthesis of the polyketide actinorhodin (act). Here we compare structural features of the polyketide synthase (PKS) ACP, determined by two-dimensional 1H-NMR, with the Escherichia coli fatty acid synthase (FAS) ACP. The PKS ACP contains four helices (residues 7-16 [A], 42-53 [B], 62-67 [C], 72-86 [D]), and a large loop (residues 17-41) having no defined secondary structure with the exception of a turn between residues 21 and 24. The act ACP shows 47% sequence similarity with the E. coli FAS ACP and the results demonstrate that the sequence homology is extended to the secondary structure of the proteins.
authors.affiliation: School of Chemistry, University of Bristol Molecular Recognition Centre, UK.
authors.forename: M P
authors.initials: MP
authors.lastname: Crump
authors.forename: J
authors.initials: J
authors.lastname: Crosby
authors.forename: C E
authors.initials: CE
authors.lastname: Dempsey
authors.forename: M
authors.initials: M
authors.lastname: Murray
authors.forename: D A
authors.initials: DA
authors.lastname: Hopwood
authors.forename: T J
authors.initials: TJ
authors.lastname: Simpson
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry*; D000217:Acyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000201:enzymology*; D064429:Fatty Acid Synthases / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D048630:Polyketide Synthases; D017433:Protein Structure, Secondary; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D048630:Polyketide Synthases; D000217:Acyltransferases; C087193:polyketide beta-ketoacylsynthase; D064429:Fatty Acid Synthases