Analysis and potential of excretory-secretory antigens of Setaria cervi for immunodiagnosis of human filariasis.
The excretory-secretory (E-LS) products released by the adult Setaria cervi, a bovine filarial parasite, were used to raise polyvalent hyperimmune serum in rabbits. Analysis of E-S products, using anti-E-S serum showed the presence of 10-14 immunogenic proteins, the rabbit anti-E-S serum showed reciprocal antibody titres in the range of 100,000-250,000 by enzyme linked immunosorbent assay. The anti-E-S antibodies could detect circulating antigen in filarial patients sera by Counter immunoelectrophoresis.
Mustafa
H
H
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Srivastava
N
N
Kaushal
D C
DC
Kaushal
N A
NA
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D002417:Cattle; D005368:Filariasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D017182:Setaria Nematode / Q000276:immunology*
D000947:Antigens, Helminth