Construction of plasmids and expression in Escherichia coli of enzymatically active fusion proteins containing the heme-domain of a P450 linked to NADPH-P450 reductase.
Fisher
C W
CW
Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas 75235, USA.
Shet
M S
MS
Estabrook
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006418:Heme / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D006418:Heme; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase