Complex cytogenetic and immunophenotypic aberrations in a patient with Sezary syndrome.
Sezary syndrome is defined as the leukemic variation of cutaneous T-cell lymphomas. Here we describe the cytogenetic pattern of peripheral T-cells of a 50-year-old male patient suffering from this disease. We used Giemsa-banding (G-banding) technique and a fluorescence in situ hybridization (FISH) assay to determine cytogenetic changes affecting 15 different chromosomes. The cells displayed an abnormal hypodiploid karyotype with a prominent insertion located at the short arm of chromosome 1. Unbalanced translocations were observed involving chromosomes 4 and 14. Besides other abnormalities we detected a 6q- deletion. These multiple genetic changes may reflect the high aggressivity of the neoplastically transformed T-cell population and the poor response to chemotherapeutic treatment.
Mohr
B
B
Universitätsklinikum Carl Gustav Carus der Technischen Universität Dresden, Germany.
Illmer
T
T
Oelschlägel
U
U
Nowak
R
R
Hölig
K
K
Paaz
U
U
Kroschinsky
F
F
Schuler
U
U
Ehninger
G
G
D000782:Aneuploidy; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D017809:Fatal Outcome; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000473:pathology*; D012751:Sezary Syndrome / Q000235:genetics* / Q000473:pathology