Differential expression of CD45RA and CD45RO molecules on human decidual and peripheral blood lymphocytes at early stage of pregnancy.
OBJECTIVE
The use of monoclonal antibodies for CD45RA and CD45RO antigens is important in defining maturational and functional stages on lymphocytes.


METHODS
To characterize distribution of two isoforms of CD45 antigen CD45RA and CD45RO on CD3+, CD4+, CD8+ and CD56+ lymphocyte subsets from first trimester human decidua, two-color flow cytometry were used.


RESULTS
In decidua, there were much higher levels of CD45RO+ and much lower levels of CD45RA+ cells among CD3+, CD4+, and CD8+ cells as compared with peripheral blood samples of the same pregnant women. Only approximately 40% of CD56+ cells in decidua expressed CD45RA. Unlike peripheral blood, approximately 30% of decidual natural killer (NK) cells weakly stained with anti-CD45RO antibodies. Double-negative CD45RA- CD45RO- NK cells were also present in decidua.


CONCLUSIONS
The significantly raised percentage of intradecidual T cells expressing CD45RO suggest decidual accumulation of antigen-committed memory cells. The patterns of CD45 isoforms expression on decidual CD56+ cells are consistent with hypothesis that uterine CD56+ lymphocytes are terminally differentiated cells of NK lineage.
Slukvin
I I
II
Laboratory of Immunology, Ukrainian Institute of Pediatrics, Obstetrics and Gynecology, Kiev.
Merkulova
A A
AA
Vodyanik
M A
MA
Chernyshov
V P
VP
D002454:Cell Differentiation / Q000276:immunology; D003656:Decidua / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000096:biosynthesis* / Q000097:blood*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First
D017493:Leukocyte Common Antigens