title: The multiple-sugar metabolism (msm) gene cluster of Streptococcus mutans is transcribed as a single operon.
abstract: The multiple-sugar metabolism (msm) locus of Streptococcus mutans constitutes a non-PTS sugar uptake system responsible for the transport and utilization of raffinose, melibiose and isomaltotrioses. While previous studies have used polar mutations to suggest that these genes are co-transcribed, there has not been evidence to support this. In this report we present direct evidence that the msm genes can be transcribed as a single operon.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA. rmclaugh@rex.uokhsc.edu
authors.forename: R E
authors.initials: RE
authors.lastname: McLaughlin
authors.forename: J J
authors.initials: JJ
authors.lastname: Ferretti
mesh_terms: D001483:Base Sequence; D001693:Biological Transport, Active / Q000235:genetics; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005798:Genes, Bacterial; D008553:Melibiose / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D011887:Raffinose / Q000378:metabolism; D013295:Streptococcus mutans / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014312:Trisaccharides / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D018076:DNA, Complementary; D012329:RNA, Bacterial; D014312:Trisaccharides; C045441:isomaltotriose; D008553:Melibiose; D011887:Raffinose