Comparison of cyclosporin A absorption from LCT and MCT solutions following intrajejunal administration in conscious dogs.
Absorption from the intestine of cyclosporin A (CsA), dissolved in either a medium-chain (MCT) or a long-chain triglyceride (LCT) solution, was investigated in a chronic dog model. Following intrajejunal administration of 20 mg of CsA/kg of body weight, absorption, judged by the portalvenous appearance of CsA, was determined by measuring whole blood CsA concentrations in the portalvenous and arterial blood and the portalvenous flow. Appearance of CsA from LCT commenced earlier and attained significantly higher mean peak values (+/- SEM) in the portalvenous blood (2557 +/- 436 ng/mL) than from MCT (274 +/- 80 ng/mL). Portalvenous concentrations of CsA were always higher than arterial concentrations for both LCT and MCT, suggesting that CsA is transported by portalvenous blood following uptake from the gut. Absorption of CsA, measured over 300 min, was 10 times higher with LCT (9.96 +/- 2.00%) than with MCT (0.95 +/- 0.21%). This significant difference is believed to result from the formation of mixed micelles which occurs during digestion of LCT but not MCT.
Behrens
D
D
Department of Surgery, University of Basel, Switzerland.
Fricker
R
R
Bodoky
A
A
Drewe
J
J
Harder
F
F
Heberer
M
M
D000818:Animals; D003243:Consciousness; D016572:Cyclosporine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004285:Dogs; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D011169:Portal Vein; D012996:Solutions; D014280:Triglycerides / Q000008:administration & dosage / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D012996:Solutions; D014280:Triglycerides; D016572:Cyclosporine