[Applications of the polymerase chain reaction (PCR) to the diagnosis of central nervous system infections].
The utility of polymerase chain reaction (PCR) is described for the diagnosis in three patients suffering from central nervous system infections, tuberculous meningitis, herpetic encephalitis and cerebral toxoplasmosis. PCR was performed in the cerebrospinal fluid after processing the specimen by two methods, proteinase K digestion and phenol extraction of DNA. Amplification was realized using primers previously described that amplify specific DNA fragments of each microorganisms (insertion sequence IS6110 of Mycobacterium tuberculosis, B1 gene of Toxoplasma gondii, and DNA polymerase gene of Herpes simplex virus). In all three cases, PCR was positive after amplification of the specimen extracted with proteinase K, as well as when a complete DNA extraction with phenol was realized. In all cases a band of amplified products was observed in agarose gels. In conclusion, in all three patients described, PCR would had allowed the diagnosis in seven hours, and PCR should be consider a rapid sensitive and relatively simple method.
Querol
J M
JM
Servicio de Medicina Interna, Hospital Lluìs Alcanyis, Xativa.
Farga
A
A
Alonso
C
C
Granda
D
D
Alcaraz
M J
MJ
García de Lomas
J
J
D017088:AIDS-Related Opportunistic Infections / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D004247:DNA / Q000134:cerebrospinal fluid; D018792:Encephalitis, Viral / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D017809:Fatal Outcome; D005260:Female; D015497:HIV-1; D006561:Herpes Simplex / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D015819:Substance Abuse, Intravenous / Q000150:complications; D016781:Toxoplasmosis, Cerebral / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid / Q000175:diagnosis*
D004247:DNA
Aplicaciones de la reacción en cadena de polimerasa (PCR) al diagnóstico de infecciones del sistema nervioso central.