title: Nuclear lamin expression in chronic hibernating myocardium in man.
abstract: Cardiomyocytes of chronic hibernating myocardium are known to undergo structural changes, indicative of dedifferentiation. Amongst these are changes in nuclear shape and chromatin distribution. Nuclear A-type lamins are known to be expressed in a differentiation-related fashion and to contribute to nuclear integrity and chromatin organization. Lamin expression was investigated with immunocytochemical staining procedures in biopsies from patients with chronic hibernating myocardium. The expression of A-type (lamin A and C) were shown to be downregulated during hibernation, while lamin B2 remained present in hibernating cardiomyocytes in a way similar to embryonic muscle cells. All heart muscle cells were shown to be negative for lamin B1. The absence of A-type lamins in chronic hibernating cardiomyocytes could be taken as an additional argument for the dedifferentiation state of these cells. The absence of A-type lamins was accompanied by dispersion of the nuclear heterochromatin, in a way similar to nuclei of embryonic cardiomyocytes.
authors.affiliation: Department of Molecular Cell Biology & Genetics, Maastricht, University of Limburg, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Ausma
authors.forename: G J
authors.initials: GJ
authors.lastname: van Eys
authors.forename: J L
authors.initials: JL
authors.lastname: Broers
authors.forename: F
authors.initials: F
authors.lastname: Thoné
authors.forename: W
authors.initials: W
authors.lastname: Flameng
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
authors.forename: M
authors.initials: M
authors.lastname: Borgers
mesh_terms: D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D001026:Coronary Artery Bypass; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D017682:Myocardial Stunning / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins