[Long-term effect of a beta-adrenergic agonist on sodium uptake into cultured rat alveolar type II cells].
To evaluate the long-term effect of a beta-adrenergic agonist on Na(+)-channel function in cultured rat alveolar type II cells, we measured 22Na+ uptake into type II cells cultured in the presence and absence of 0.1 mM terbutaline for up to 5 days. Terbutaline did not influence the growth, morphology, or protein content of the cells. Terbutaline increased amiloride-inhibitable 22Na+ uptake into the cells at day 2 in culture, but there was no significant difference at day 5. Because amiloride-inhibitable 22Na+ uptake reflects Na(+)-channel function, these data indicate that terbutaline may transiently upregulate Na(+)-channel function. This study provides direct evidence of a long-term effect of a beta-adrenergic agonist on Na(+)-channel function in alveolar type II cells.
Suzuki
S
S
Department of Thoracic Surgery, Tohoku University, Sendai, Japan.
Sakuma
T
T
Koike
K
K
Ono
S
S
Tanita
T
T
Fujimura
S
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D017136:Ion Transport / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000493:pharmacokinetics*; D015222:Sodium Channels / Q000378:metabolism*; D013726:Terbutaline / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; D015222:Sodium Channels; D000584:Amiloride; D012964:Sodium; D013726:Terbutaline