Differential phenotypic properties of human peripheral blood CD56dim+ and CD56bright+ natural killer cell subpopulations.
We investigated the presence of integrins and various other cell surface markers on the surface of freshly isolated CD56dim+ and CD56bright+ NK cells, the latter comprising a mean of 6.5% of total peripheral blood (PB) CD3-CD56+ NK cells. This small subpopulation stained more intensively for CD2, CD11c, CD15s, CD44, CD49e, CD55, CD62L, HLA-DR, and GZS-1, whereas there was no expression of CD49f in contrast to CD56dim+ cells. The myeloid antigen CDw65 was present on 11% of CD56bright+ cells, other myeloid markers were not found. 28% of CD56bright+ cells were positive for CD45R0. These results support the notion that CD56bright+ NK cells, based on their different marker profile, may possess different functional and biodistributional properties and--due to the signal-transducing capabilities of several adhesion molecules--differential patterns of stimulatability compared to the majority of PB-NK cells.
Sedlmayr
P
P
Department of Internal Medicine, Karl Franzens University, Graz, Austria.
Schallhammer
L
L
Hammer
A
A
Wilders-Truschnig
M
M
Wintersteiger
R
R
Dohr
G
G
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000145:classification* / Q000276:immunology*; D008297:Male
D015703:Antigens, CD; D015415:Biomarkers; D019002:CD56 Antigen; D015815:Cell Adhesion Molecules