Molecular evidence for increased hematopoietic proliferation in the spleen of the b/b laboratory rat.
The splenomegaly and the appearance of a significant number of CFU-E (erythroid colony-forming units) and BFU-E1 (erythroid burst-forming units) in the Belgrade laboratory rat (b/b) spleen prompted us to analyse further the molecular evidence for increased hematopoietic proliferation in the b/b spleen. Messenger RNAs (mRNAs) specific for globins, proteins for iron transport and deposition and the band 3 protein were used in rat erythropoietic tissues as markers for proliferation and erythroid differentiation. In the b/b spleen, all mRNAs analysed display an erythroid-specific pattern of expression. This analysis also revealed an enhanced level of mRNA for ferritin in the +/b spleen, whereas erythrocyte-specific mRNA production was normal.
Savković
S
S
Institute of Molecular Genetics and Genetic Engineering, Beograd, (Yugoslavia).
Pavlović
S
S
Mitrović
T
T
Joksimović
M
M
Marjanović
J
J
Glisin
V
V
Popović
Z
Z
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D002455:Cell Division; D015342:DNA Probes; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis; D005293:Ferritins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005914:Globins / Q000235:genetics; D006411:Hematopoiesis, Extramedullary; D006579:Heterozygote; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000235:genetics; D013154:Spleen / Q000378:metabolism / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; D015342:DNA Probes; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D005914:Globins; D005293:Ferritins