Serotyping of Cryptococcus neoformans by dot enzyme assay.
A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.
Belay
T
T
Department of Chemistry, Georgia State University, Atlanta 30303, USA.
Cherniak
R
R
O'Neill
E B
EB
Kozel
T R
TR
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D003455:Cryptococcus neoformans / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D011134:Polysaccharides / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011817:Rabbits; D012703:Serotyping / Q000379:methods*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C027478:glucuronoxylomannan