AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.
An early step in the initiation of polyomavirus DNA replication is viral large-T-antigen-mediated unwinding of the origin. We report that components of the AP1 transcription factor, Fos and Jun, interact with T antigen in vitro to enhance unwinding of the viral origin. This provides a biochemical basis for the capacity of AP1 to activate viral DNA replication in vivo.
Guo
W
W
Department of Biochemistry, University of Missouri--Columbia 65202, USA.
Tang
W J
WJ
Bu
X
X
Bermudez
V
V
Martin
M
M
Folk
W R
WR
D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D016761:Oncogene Proteins v-fos / Q000378:metabolism / Q000494:pharmacology; D010544:Peroxidases; D054464:Peroxiredoxins; D011120:Polyomavirus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism* / Q000494:pharmacology; D014779:Virus Replication / Q000235:genetics*
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D016761:Oncogene Proteins v-fos; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D010544:Peroxidases; D054464:Peroxiredoxins