HIV-1 proviral DNA sequences of env gp41 PCR amplificates from Tanzania.
The polymerase chain reaction (PCR) and direct DNA sequencing were used to detect and characterize selected regions of the HIV-1 proviral genome in whole blood samples from Tanzania. Specific PCR amplification products were obtained in gag and/or env (gp41) regions from 15 of the 19 HIV-1 seropositive samples investigated. Env regions from 12 different amplificates were further characterized using the dideoxy sequencing method. Preliminary results indicate that, despite scattered nucleotide mismatches, HIV-1 gp41 amino acid sequences from Tanzania conform to the 1990 Los Alamos African consensus sequence and resemble the HIV-1 subtype A or D consensus sequences in the characterized regions.
Holm-Hansen
C
C
Centre for International Health, Gade Institute, University of Bergen, Norway.
Ayehunie
S
S
Johansson
B
B
Nkya
W
W
Shao
J
J
Haukenes
G
G
D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D005002:Ethiopia; D015752:Genes, env; D015700:HIV Envelope Protein gp41 / Q000097:blood / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D013636:Tanzania
D004279:DNA, Viral; D015700:HIV Envelope Protein gp41