title: Glutathione status in diabetes-induced embryopathies.
abstract: The mechanism involved in diabetes-induced embryotoxicity is still unclear. Mitochondrial alterations probably produced by oxidative stress have been described in embryos developing in a diabetic environment. Furthermore, oxygen radicals-scavenging enzymes can reduce the embryotoxic effects induced by diabetic conditions. In this work we tried to test if glutathione (GSH), a tripeptide implicated in cellular protection against reactive oxygen species, is involved in diabetes-related embryotoxicity. Rat embryos were explanted on day 11 on gestation from normal and from streptozotocin-diabetic mothers. The embryos were examined morphologically, then protein, DNA and GSH were determined both in embryos and in their visceral yolk sacs. The embryos explanted from diabetic mothers showed signs of developmental retardation and 16% were morphologically abnormal. GSH content was reduced in these embryos in comparison to control, but the GSH/protein in the visceral yolk sacs of conceptuses explanted from diabetic mothers was higher than in control visceral yolk sacs. Our hypothesis is that the reduction of embryonic GSH is a consequence of the alteration in GSH transport across the yolk sac endodermal cells damaged by diabetic conditions. The observed reduction in embryonic GSH could reduce the protection against the oxidative stress condition described in diabetic pathology.
authors.affiliation: Department of Biology, University of Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Menegola
authors.forename: M L
authors.initials: ML
authors.lastname: Broccia
authors.forename: M
authors.initials: M
authors.lastname: Prati
authors.forename: R
authors.initials: R
authors.lastname: Ricolfi
authors.forename: E
authors.initials: E
authors.lastname: Giavini
mesh_terms: D000818:Animals; D001692:Biological Transport; D000013:Congenital Abnormalities / Q000209:etiology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005260:Female; D005315:Fetal Diseases / Q000209:etiology*; D005333:Fetus / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D018384:Oxidative Stress; D011247:Pregnancy; D011254:Pregnancy in Diabetics; D051381:Rats; D015017:Yolk Sac / Q000378:metabolism
chemical_list: D005978:Glutathione