title: Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells.
abstract: We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin, and also from all other actin isoforms. We show, by immunofluorescence and immunoperoxidase microscopy of tissue sections, that cardiac/fetal alpha-actin can be localized not only in cardiomyocytes but also in skeletal muscles and their satellite cells during regeneration. These mAbs are potentially valuable in developmental biology, for the characterization of tissue and cultured myogenic cells, in pathology, and for serodiagnosis.
authors.affiliation: Division of Cell Biology, German Cancer Research Center, Herdelberg, Germany.
authors.forename: W W
authors.initials: WW
authors.lastname: Franke
authors.forename: S
authors.initials: S
authors.lastname: Stehr
authors.forename: S
authors.initials: S
authors.lastname: Stumpp
authors.forename: C
authors.initials: C
authors.lastname: Kuhn
authors.forename: H
authors.initials: H
authors.lastname: Heid
authors.forename: H R
authors.initials: HR
authors.lastname: Rackwitz
authors.forename: M
authors.initials: M
authors.lastname: Schnölzer
authors.forename: R
authors.initials: R
authors.lastname: Baumann
authors.forename: H J
authors.initials: HJ
authors.lastname: Holzhausen
authors.forename: R
authors.initials: R
authors.lastname: Moll
mesh_terms: D000199:Actins / Q000032:analysis*; D000911:Antibodies, Monoclonal; D009202:Cardiomyopathies / Q000378:metabolism / Q000473:pathology; D005326:Fetal Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D012038:Regeneration / Q000502:physiology*; D012698:Serologic Tests
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D005326:Fetal Proteins