Differential diagnoses of influenza A virus, influenza B virus, and respiratory syncytial virus infections by direct immunofluorescence using mixtures of monoclonal antibodies of different isotypes.
Differences between isotypes of monoclonal antibodies were employed to detect influenza A and B viruses and respiratory syncytial virus by direct immunofluorescence using fluorescein isothiocyanate or Texas Red conjugates. Examination of 56 specimens for influenza A and B viruses and 112 specimens for influenza A virus and respiratory syncytial virus showed the mixed-isotype test to be comparable to the conventional procedure.
Murphy
P
P
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
Roberts
Z M
ZM
Waner
J L
JL
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007132:Immunoglobulin Isotypes; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses / Q000276:immunology; D014966:Xanthenes
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D005456:Fluorescent Dyes; D007132:Immunoglobulin Isotypes; D014966:Xanthenes; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate