title: Novel beta-D-galactofuranose-containing high-mannose type oligosaccharides in ascorbate oxidase from Acremonium sp. HI-25.
abstract: Ascorbate oxidase from the fungus Acremonium sp. HI-25 is a copper-containing glycoprotein that catalyzes the oxidation of ascorbic acid to dehydroascorbic acid. Monosaccharide composition analysis showed that the enzyme contains exclusively N-linked oligosaccharide chains. Following liberation by hydrazinolysis/re-N-acetylation, and fractionation by HPLC on anion exchange. Amide-80 and/or octadecyl silica columns after derivatization with p-aminobenzoic ethyl ester, the structures of the twelve major neutral oligosaccharides were identified by FAB-MS, 400 MHz 1H-NMR, methylation analysis, mild acid hydrolysis, and/or sequential exoglycosidase digestions. Acremonium sp. ascorbate oxidase was found to consist of high-mannose type oligosaccharides (76.3%) having 4 to 9 mannose residues and a series of novel D-galactofuranose-containing high-mannose type oligosaccharides (18.6%) with the following structure.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: S
authors.initials: S
authors.lastname: Emi
authors.forename: H
authors.initials: H
authors.lastname: Iwamoto
authors.forename: J
authors.initials: J
authors.lastname: Hirose
authors.forename: K
authors.initials: K
authors.lastname: Hiromi
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Shin
authors.forename: S
authors.initials: S
authors.lastname: Murao
authors.forename: F
authors.initials: F
authors.lastname: Matsuura
mesh_terms: D000164:Acremonium / Q000201:enzymology* / Q000378:metabolism; D001204:Ascorbate Oxidase / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009844:Oligosaccharides; D001204:Ascorbate Oxidase; D008358:Mannose; D005690:Galactose