Protection of rabbits against rabbit viral haemorrhagic disease with a vaccinia-RHDV recombinant virus.
In order to protect domestic and wild rabbits against RVHD, we constructed a recombinant vaccinia-RHDV virus, using the Copenhagen strain of the vaccinia virus. This recombinant virus expressed the RHDV capsid protein (VP60). Analysis of the expressed product showed that the recombinant protein, which is 60 kDa in size, was antigenic as revealed by its reactions in immunoprecipitation and indirect immunofluorescence with the antibodies raised against RHDV. The recombinant virus induced high level of RHDV specific antibodies in rabbits following immunization. Inoculations by both the intradermal and oral routes allow protection of animals against a challenge with virulent RHDV.
Bertagnoli
S
S
Laboratoire INRA/ENVT de microbiologie moléculaire, Toulouse, France.
Gelfi
J
J
Petit
F
F
Vautherot
J F
JF
Rasschaert
D
D
Laurent
S
S
Le Gall
G
G
Boilletot
E
E
Chantal
J
J
Boucraut-Baralon
C
C
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D017250:Caliciviridae Infections / Q000517:prevention & control*; D002460:Cell Line; D016679:Genome, Viral; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D007271:Injections, Intradermal; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus