Expression of recombinant parvovirus NS1 protein by a baculovirus and application to serologic testing of rodents.
A recombinant baculovirus containing the NS1 gene of minute virus of mice was constructed. Optimal expression of the recombinant NS1 protein (rNS1) was achieved by infecting Trichoplusa ni High Five cells at a multiplicity of 10 and incubating them for 72 h postinfection. An enzyme-linked immunosorbent assay (ELISA) with rNS1 as the antigen was evaluated for serologic testing of laboratory rodents. The rNS1 ELISA proved to be a more sensitive method for the detection of antibodies to recently recognized rodent parvovirus species (mouse orphan parvovirus and rat orphan parvovirus) and prototypic parvovirus species (minute virus of mice, Kilham's rat virus, and H-1) than were conventional parvovirus ELISAs that use whole parvovirus virions.
Riley
L K
LK
Department of Veterinary Pathobiology, University of Missouri, Columbia 65211, USA. vmlelar@vetmed.vetmed.missouri.edu
Knowles
R
R
Purdy
G
G
Salomé
N
N
Pintel
D
D
Hook
R R
RR
Franklin
C L
CL
Besch-Williford
C L
CL
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D005822:Genetic Vectors; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics; D010322:Parvoviridae Infections / Q000276:immunology; D017992:Parvovirus / Q000235:genetics* / Q000276:immunology*; D016133:Polymerase Chain Reaction; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D013045:Species Specificity; D018411:Spodoptera; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology*
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; C081367:NS1 protein, minute virus of mice; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins