Lack of major changes in ATPase activity in mitochondria from liver, heart, and skeletal muscle of rats upon ageing.
ATP hydrolase activity has been investigated in mitochondria from liver, heart, and skeletal muscle from adult (6 months) and aged (24 months) rats. No significant changes in total ATPase activity were observed in the three tissues, but the oligomycin sensitivity was slightly decreased in heart mitochondria of aged rats. The bicarbonate-induced stimulation of hydrolytic activity was somewhat decreased in mitochondria from aged rats, particularly in liver. No significant change was observed in ATPase activity after release of the endogenous inhibitor protein, IF1. It is concluded that no activity changes to be directly ascribed to the catalytic sector F1 of the enzyme occur upon ageing, but it cannot be excluded that changes in the membrane sector F0 occur as a consequence of mtDNA mutations.
Barogi
S
S
Dipartimento di Biochimica G. Moruzzi, University of Bologna, Italy.
Baracca
A
A
Parenti Castelli
G
G
Bovina
C
C
Formiggini
G
G
Marchetti
M
M
Solaini
G
G
Lenaz
G
G
D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D009840:Oligomycins; D000251:Adenosine Triphosphatases