Mouse euchromatin specific "genome-painting" with a LINE probe: a rapid method for identification and mapping of human chromosomes in mouse-human microcell hybrids by two-color FISH.
We describe the use of a long interspersed repetitive sequence (mCPE1.51) for mouse euchromatin specific "genome-painting". In fluorescence in situ hybridization (FISH) experiments, this probe was suitable for identification of the mouse genome and disclosure of translocations of mouse chromosome segments to chromosomes of different species without suppression hybridization. The euchromatin specificity of the probe allowed the discrimination between euchromatin and heterochromatin of mouse chromosomes. Simultaneous hybridization of the biotinylated mouse specific genome-painting probe and a digoxigenin-labeled human chromosome 3-specific cosmid probe to metaphase spreads of mouse-human microcell hybrid carrying a single deleted human chromosome 3 on a mouse fibrosarcoma background, allowed rapid identification and mapping of human chromosome 3.
Katona
R
R
Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary.
Szeles
A
A
Hadlaczky
G
G
D000818:Animals; D002843:Chromatin / Q000502:physiology*; D002874:Chromosome Mapping; D015342:DNA Probes; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D013997:Time Factors
D002843:Chromatin; D015342:DNA Probes