Analysis of anchor residues in a naturally processed HLA-DR53 ligand.
The peptide motif of the HLA-DR53 (DRB4(*)0101) molecule, which is associated with autoimmune diseases including Vogt-Koyanagi-Harada's syndrome, was determined by peptide binding assay using human L plastin p581 - 595 peptide and its substituted analogues. L plastin p581 - 595 peptide is one of the naturally processed peptides bound to HLA-DR9/DR53 (DRB1(*)0901/DRB4(*)0101) molecules. The binding affinity of each peptide to the HLA-DR53 molecule was measured by fluorescence intensity of biotinylated peptides to L cell transfectants expressing HLA-DR53 molecules, followed by treatment with avidin-fluorescence. Binding of biotinylated peptides to HLA-DR53 molecules was not inhibited by all single-alanine-substituted nonbiotinylated peptides, indicating that the replaced position was important for binding to the HLA-DR53 moleule. The inhibitory motif is considered to be an HLA-DR53-specific binding motif, composed of a positively charged residue (K) at position 1, a hydrophobic residue (I) at position 4, positively charged residue (R or K) at position 8 or 9, and another hydrophobic residue (I) at position 10. This predicted motif is different from the binding motifs of other HLA-DR molecules.
Kobayashi
H
H
Department of Pathology, Asahikawa Medical College, Nishikagura 4-5-3-11, Asahikawa 078, Japan.
Kokubo
T
T
Abe
Y
Y
Sato
K
K
Kimura
S
S
Miyokawa
N
N
Katagiri
M
M
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D001327:Autoimmune Diseases / Q000276:immunology; D006684:HLA-DR Antigens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D059845:HLA-DRB4 Chains; D006801:Humans; D007739:L Cells; D008024:Ligands; D008562:Membrane Glycoproteins; D051379:Mice; D008840:Microfilament Proteins; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014607:Uveomeningoencephalitic Syndrome / Q000276:immunology
D006684:HLA-DR Antigens; C050610:HLA-DR53; D059845:HLA-DRB4 Chains; D008024:Ligands; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C026742:plastin