Alterations of GABAB binding caused by acute and chronic lead administration.
The effect of lead on GABAB binding was studied on membranes obtained from acute and chronically lead-treated rats. Acute lead treatment reduced both GABAB affinity (KD) by about 30% and density of receptor (Bmax) by about 15%. On the contrary chronic lead treatment increased receptor capacity by about 20% in spite of decreased receptor affinity by 25%. Both acute and chronic lead treatment shiffted displacement curves toward higher concentration of non-labelled compound (decreased affinity). The results show that lead can affect the GABAB binding in two ways: by reducing affinity of binding and by altering capacity of binding.
Waśkiewicz
J
J
Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D007854:Lead / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018080:Receptors, GABA-B / Q000187:drug effects / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D018080:Receptors, GABA-B; D007854:Lead