Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene.
We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2-dichloroethylene very efficiently.
Suyama
A
A
Department of Agricultural Chemistry, Kyushu University, Japan.
Iwakiri
R
R
Kimura
N
N
Nishi
A
A
Nakamura
K
K
Furukawa
K
K
D001555:Benzene Derivatives / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism; D007506:Iron-Sulfur Proteins; D010105:Oxygenases / Q000235:genetics*; D015202:Protein Engineering; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D014050:Toluene / Q000378:metabolism; D014241:Trichloroethylene / Q000378:metabolism*
D001555:Benzene Derivatives; D001713:Biphenyl Compounds; D007506:Iron-Sulfur Proteins; D010105:Oxygenases; D011993:Recombinant Fusion Proteins; D014050:Toluene; D014241:Trichloroethylene; C010574:biphenyl; C023172:toluene dioxygenase; C074161:biphenyl-2,3-dioxygenase