Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl.
Burgess
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McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, 53706, USA.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001433:Bacteriolysis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D003902:Detergents / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D002479:Inclusion Bodies / Q000737:chemistry; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D012808:Sigma Factor / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012995:Solubility
D001426:Bacterial Proteins; D003902:Detergents; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; C025231:sarkosyl; D012321:DNA-Directed RNA Polymerases; D012521:Sarcosine