title: Age-related decrease in the inductability of heat shock protein 72 in normal human skin.
abstract: In order to know the effect of chronological ageing on the induction of heat shock protein (HSP) in normal human skin, the 72-kDa HSP (HSP72) was examined in organ-cultured samples of normal human skin which were obtained from 30 individuals (age range 17-86 years). The skin explants were first incubated at 37 degrees C for 24 h, and then heat-treated at 45 degrees C for 1 h. After heat treatment, the skin explants were incubated at 37 degrees C for 1 or 3 h. Immunohistological analysis, using a monoclonal antibody specific for the HSP72, revealed that although the time course of the heat-induced HSP72 expression was similar in the young and aged groups, a lower level of induction of HSP72 was observed in the aged group. This result indicates that there is an age-related dysfunction of the heat shock response in normal human skin.
authors.affiliation: Department of Dermatology, Nara Medical University, Kashihara, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: M
authors.initials: M
authors.lastname: Hatoko
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: T
authors.initials: T
authors.lastname: Shirai
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D012867:Skin / Q000378:metabolism*
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins