DNA-nuclease activity of the single-chain ribosome-inactivating proteins dianthin 30, saporin 6 and gelonin.
The single-chain ribosome-inactivating proteins (scRIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.
Roncuzzi
L
L
Department of Experimental Pathology, University of Bologna, Italy.
Gasperi-Campani
A
A
D000998:Antiviral Agents / Q000378:metabolism*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000922:Immunotoxins; D015636:Magnesium Chloride / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism*; D015183:Restriction Mapping; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000378:metabolism; D000076985:Saporins; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D000998:Antiviral Agents; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004279:DNA, Viral; D000922:Immunotoxins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; D015636:Magnesium Chloride; D012965:Sodium Chloride; C025674:GEL protein, Gelonium multiflorum; D003851:Deoxyribonucleases; D009699:N-Glycosyl Hydrolases; D000076985:Saporins