Enzymatic degradation of cell walls of apples and characterization of solubilized products.
Partially depectinated apple cell walls were digested by pectin lyase or endoglucanase or a combination. By combining these commercial enzymes, a higher yield of 22.2% of carbohydrate material was obtained compared with only 13.9% and 5.7%, respectively, when using them singly. Only small amounts of carbohydrates were extracted by buffer (0.8%). The solubilized extracts were fractionated using a combination of ion-exchange chromatography and gel filtration. The individual subfractions were analysed for neutral sugar and uronic acid content. The results indicated the existence of a synergistic effect between pectin lyase and endoglucanase based on the percentage of material extracted.
Capek
P
P
Laboratoire de Biochimie et Technologie des Glucides, Institut National de la Recherche Agronomique, Nantes, France.
Renard
C M
CM
Thibault
J F
JF
D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004357:Drug Synergism; D005638:Fruit / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D012995:Solubility; D014574:Uronic Acids / Q000032:analysis
D002241:Carbohydrates; D014574:Uronic Acids; D002480:Cellulase; D011133:Polysaccharide-Lyases; C027349:pectin lyase