Murine splenocyte proliferation by porin of Shigella dysenteriae type 1 and inhibition of bacterial invasion of HeLa cell by anti-porin antibody.
The purified porin of Shigella dysenteriae type 1 showed strong mitogenic activity for murine splenocytes. Preincubation of S. dysenteriae type 1 with anti-porin antibody reduced the bacterial plaque formation in HeLa cell monolayers by 45%. The two immunobiological activities indicate that porin might be important in the induction of protective immunity against shigellosis.
Roy
S
S
Division of Immunology and Vaccine Development, National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, West Bengal, India.
Biswas
T
T
D000818:Animals; D000907:Antibodies, Bacterial; D002455:Cell Division; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018272:Porins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012762:Shigella dysenteriae / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity*; D013154:Spleen / Q000166:cytology*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D008934:Mitogens; D018272:Porins