title: Acceptor specificity of cyclodextrin glucanotransferase from an alkalophilic Bacillus species and synthesis of glucosyl rhamnose.
abstract: Cyclodextrin glucanotransferase [1,4-alpha-D-glucan 4-alpha-D-(1,4-alpha-D-glucano)-transferase (cyclizing), EC 2. 4. 1. 19] from an alkalophilic Bacillus species A2-5a had a wider acceptor specificity than that from B. macerans, which was similar to those from B. stearothermophilus and B. circulans. Glucosyl rhamnose produced by the CGTase was identified as glucopyranosyl-alpha-1,4-rhamnopyranose by alpha- and beta-glucosidase treatments, and 1H- and 13C-NMR analyses.
authors.affiliation: Biochemical Research Laboratory, Ezaki Glico Co. Ltd., Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kometani
authors.forename: Y
authors.initials: Y
authors.lastname: Terada
authors.forename: T
authors.initials: T
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Nakae
authors.forename: H
authors.initials: H
authors.lastname: Takii
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D004187:Disaccharides; C103234:glucopyranosyl-alpha-L-rhamnopyranose; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase