title: Antifungal activity from Alpinia galanga and the competition for incorporation of unsaturated fatty acids in cell growth.
abstract: An antimicrobial diterpene was isolated from Alpinia galanga in the screening for potentiators of phytochemical antibiotic action. The structure was elucidated by spectral data and identified as (E)-8 beta, 17-epoxylabd-12-ene-15, 16-dial (1). Diterpene 1 synergistically enhanced the antifungal activity of quercetin and chalcone against Candida albicans. Antifungal activity of 1 was reversed by unsaturated fatty acids. Protoplasts of C. albicans were lysed by 1. These results suggest that antifungal activity of 1 is due to a change of membrane permeability arising from membrane lipid alternation.
authors.affiliation: Faculty of Engineering, Fukuyama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Haraguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kuwata
authors.forename: K
authors.initials: K
authors.lastname: Inada
authors.forename: K
authors.initials: K
authors.lastname: Shingu
authors.forename: K
authors.initials: K
authors.lastname: Miyahara
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: A
authors.initials: A
authors.lastname: Yagi
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001210:Asia, Southeastern; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D004224:Diterpenes; D005230:Fatty Acids, Nonesterified; D009281:Naphthalenes; D013141:Spiro Compounds; C081562:8,17-epoxylabd-12-ene-15,16-dial