Heterologous expression of sarcoplasmic reticulum Ca(2+)-ATPase.
In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca(2+)-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.
Reis
E M
EM
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
Slayman
C W
CW
Verjovski-Almeida
S
S
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D016297:Mutagenesis, Site-Directed; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D011994:Recombinant Proteins; D000252:Calcium-Transporting ATPases