[The proliferative response of T cells against cruzipain in chronic chagasic cardiopathy].
In this paper, we sought to determine if chronic chagasic patients with cardiopathy could be distinguished from those displaying non-chagasic cardiopathy on the basis of T cell proliferative responses to cruzipain (GP57/51), a major antigen of T. cruzi. Assays were performed with peripheral blood mononuclear cells from 24 individuals classified as follows: normal donors (n = 8), patients with non-chagasic cardiopathy (n = 8), patients with chronic chagasic cardiopathy without morbid associations (n = 8). The analysis of variance indicated that the proliferative responses stimulated by cruzipain were significantly higher in the group of chagasic patients (p = 0.0001). Turkey's multiple comparison test showed that the proliferative index medium from normal and non-chagasic cardiopathy was not significantly different from each other. We conclude that the T cell responses against T. cruzipain, as measured by proliferative indices of cells found in peripheral blood, are exclusively associated with Chagas, disease. In view of the abundance of cruzipain antigen in amastigotes it is possible that these T cell specificities contribute to the heart tissue damage observed in chronic Chagas, disease patients.
Pedrosa
R C
RC
Serviço de Cardiologia, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro.
Saad
E A
EA
Scharfstein
J
J
Lima
A M
AM
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D002598:Chagas Cardiomyopathy / Q000276:immunology*; D002908:Chronic Disease; D003546:Cysteine Endopeptidases / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D008875:Middle Aged; D015800:Protozoan Proteins; D013601:T-Lymphocytes / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology*; D018487:Ventricular Dysfunction, Left / Q000276:immunology
D000953:Antigens, Protozoan; D018984:Epitopes, T-Lymphocyte; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain
Reposta proliferativa das células T contra a cruzipaina na cardiopatia chagásica crônica.