[Effect of the length of the loop segment of local mRNA secondary structure in the region of lacZ gene translation initiation on its expression].
A number of p50FNL plasmids with a polycistronic operon IX-VIII-IFN-LacZ have been constructed. These plasmids provide the synthesis of polycistronic mRNA translational terminator of gene IFN situated in the coding region of lacZ gene at 41 nucleotide downstreams from its initiation codon. The coding region of IFN gene has been altered by insertions, substitutions, and deletions which provided the formation in the region of local secondary structures differing only by the loop size. The effect of this characteristic of mRNA secondary structure on lacZ translation efficiency has been assessed by measuring the levels of beta-Gal synthesis in E. coli harboring the corresponding plasmids. The level of lacZ expression increased with increase of the loop size. These data confirm that the rate of formation of mRNA secondary structure in the region of translation initiation has a crucial effect on the translation efficacy.
Nikolenko
G N
GN
Kravchenko
V V
VV
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D013728:Terminator Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D012333:RNA, Messenger; D001616:beta-Galactosidase
Vliianie dliny petlevogo uchastka lokal'noĭ vtorichnoĭ struktury mRNK v raĭone initsiatsii transliatsii gena LacZ na ego ékspressiiu.