title: Molecular cloning of the human rad gene: gene structure and complete nucleotide sequence.
abstract: We have isolated and sequenced human genomic DNA clones encoding the Ras-related GTP-binding protein, Rad. The gene spans 3.75 kb and consists of five exons and four introns. Translation initiates from the first of two in-frame methionine residues in the second exon. Several potential transcription cis-elements were revealed throughout the 1.7 kb 5'-flanking region, including 'E box' and CArG binding sites for regulators of transcription in muscle.
authors.affiliation: Research Division, Joslin Diabetes Center, Boston, MA, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Caldwell
authors.forename: J S
authors.initials: JS
authors.lastname: Moyers
authors.forename: A
authors.initials: A
authors.lastname: Doria
authors.forename: C
authors.initials: C
authors.lastname: Reynet
authors.forename: R C
authors.initials: RC
authors.lastname: Kahn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D019204:GTP-Binding Proteins / Q000235:genetics*; D005796:Genes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D018631:ras Proteins
chemical_list: C084355:RRAD protein, human; D019204:GTP-Binding Proteins; D018631:ras Proteins