Effect of iron (II) on the generation of hydroxyl free radicals in rat myocardium.
We examined the effect of iron (II) on the generation of hydroxyl radicals (.OH) in the extracellular fluid of rat myocardium. Salicylic acid in Ringer's solution (0.5 nmole x microliter(-1) x min(-1)) was directly infused through a microdialysis probe to detect the generation of OH as reflected by the formation of dihydroxybenzoic acid (DHBA) in the myocardium. Iron clearly produced a dose-dependent increase in .OH formation. A positive linear correlation between iron (II) and the formation of 2,3-DHBA (R2 = 0.970) or 2.5-DHBA (R2 = 0.983) was observed. However, when desferrioxamine (DES) was infused through a dialysis probe, a marked increase in DHBA formation was obtained. The present results suggest that iron (III) may reduce .OH formation by the Fenton reaction.
Obata
T
T
Department of Pharmacology, Oita Medical University, Japan.
Yamanaka
Y
Y
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects*; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D017665:Hydroxyl Radical; D007501:Iron