Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433.
The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [Table: see text]
Robijn
G W
GW
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
Gutiérrez Gallego
R
R
van den Berg
D J
DJ
Haas
H
H
Kamerling
J P
JP
Vliegenthart
J F
JF
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D014574:Uronic Acids
D009005:Monosaccharides; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids