Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR.
Wang
F
F
Department of Biochemistry and Biophysics, Texas A&M University, Houston 77030-3303, USA.
Kan
M
M
McKeehan
W L
WL
D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods*; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics; D013698:Templates, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D017468:Receptors, Fibroblast Growth Factor; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases