Effect of a phenylethanolamine N-methyltransferase inhibitor, 2,3-dichloro-alpha-methylbenzylamine, on the alpha-2-adrenoceptor function in the hypothalamus in rats.
This study examines the in vivo effects of 2,3-dichloro-alpha-methylbenzylamine (DCMB), a centrally active phenylethanolamine-N-methyltransferase (PNMT) inhibitor, on alpha 2-adrenoceptors. Sixty-six rats received either DCMB, SKF29661, the peripheral PNMT inhibitor, at a dose of 50 mg/kg or saline intraperitoneally. To examine the functional responses of the alpha 2-adrenoceptors to these drugs, changes in spontaneous locomotor activity, concentration of 3-methoxy-4-hydroxyphenylglycol (MHPG) in dialysates from the hypothalamus, and the influence of clonidine on growth hormone (GH) secretion were investigated. (1) DCMB increased spontaneous motion of the rat. (2) DCMB increased extracellular MHPG concentration in the hypothalamus verified by micro-brain dialysis. (3) Clonidine failed to stimulate GH secretion in DCMB-treated rats. These results suggest that DCMB has an inhibitory action on the alpha 2-adrenoceptor function in vivo.
Kuboia
M
M
Department of Neuropsychiatry, Yamanashi Medical University, Japan.
Atobe
M
M
Nakagawara
M
M
Kariya
T
T
D000818:Animals; D001596:Benzylamines / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects*; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D010625:Phenylethanolamine N-Methyltransferase / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects*; D013997:Time Factors
D001596:Benzylamines; D004791:Enzyme Inhibitors; D018341:Receptors, Adrenergic, alpha-2; C002569:2,3-dichloro-alpha-methylbenzylamine; D010625:Phenylethanolamine N-Methyltransferase