A partially automated pretreatment module for routine analyses for seventeen non-steroid antiinflammatory drugs in race horses using gas chromatography/mass spectrometry.
A partially automated module for the routine determination of illicit non-steroid antiinflammatory drugs (NSAIDs) in biological fluids from race horses was built, tested, refined, and shown to work. This pretreatment module retains 17 NSAIDs on an Amberlite XAD-2 column before back-elution derivatization with methyl iodide in acetonitrile. Methylated derivatives are manually injected into a gas chromatograph connected to a mass spectrometer. The quantification limits thus achieved are 50-100 ng/mL in 1 mL of urine or plasma. The proposed method is more expeditious than its manual liquid-liquid and liquid-solid extraction counterparts and is similar in speed to a typical gas chromatographic program.
Cárdenas
S
S
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Gallego
M
M
Valcárcel
M
M
Ventura
R
R
Segura
J
J
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000652:urine; D001322:Autoanalysis; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal