title: Inhibition of phospholipase C gamma 1 activity by amentoflavone isolated from Selaginella tamariscina.
abstract: Amentoflavone was isolated as an inhibitor of phospholipase C gamma 1 (PLC gamma 1) and phosphoinositides (PI)-turnover in PLC gamma 1 overexpressing NIH3T3 fibroblasts (NIH3T3 gamma 1) from Selaginella tamariscina (Selaginellaceae) together with other related biflavonoids, isocryptomerin and cryptomerin B. Only amentoflavone inhibited the PLC gamma 1 activity with an IC50 of 29 microM and the formation of total inositol phosphates (IPt) in PDGF-stimulated NIH3T3 gamma 1 with an IC50 of 9.2 microM but did not show inhibitor activity against protein kinase C. Isocryptomerin and cryptomerin B did not show inhibitor activity against PLC gamma 1 at the concentration of 150 microM, and did not inhibit IPt production in PDGF-induced NIH3T3 gamma 1 at the concentration of 180 microM.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: W K
authors.initials: WK
authors.lastname: Oh
authors.forename: B Y
authors.initials: BY
authors.lastname: Kim
authors.forename: S C
authors.initials: SC
authors.lastname: Ahn
authors.forename: D O
authors.initials: DO
authors.lastname: Kang
authors.forename: D I
authors.initials: DI
authors.lastname: Shin
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: T I
authors.initials: TI
authors.lastname: Mheen
authors.forename: J S
authors.initials: JS
authors.lastname: Ahn
mesh_terms: D016475:3T3 Cells; D000818:Animals; D044946:Biflavonoids; D002417:Cattle; D002531:Cerebellum / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D051379:Mice; D051966:Phospholipase C gamma; D008517:Phytotherapy; D010946:Plants, Medicinal; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
chemical_list: D044946:Biflavonoids; D004791:Enzyme Inhibitors; D005419:Flavonoids; D007527:Isoenzymes; C011164:amentoflavone; D011493:Protein Kinase C; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D019311:Staurosporine