Characterization of a new human transitional cell carcinoma cell line from the renal pelvis, RTCC-1/KMC.
Tumors of the renal pelvis and ureter are relatively common malignancies in Taiwan. Studying the genetic or biochemical aberrations is a feasible pursuit that has great potential to further our understanding of urothelial cancer and may provide clinically valuable information. We now report a new long-term culture (RTCC-1/KMC) of human TCC derived from the renal pelvis, which is aimed to be used as a target for those studying in this field. The cultured cells exhibited anchorage independence and loss of contact inhibition. Chromosomal analysis revealed an aneuploidy line with a modal number of 50. Population doubling time was about 36 hours at the third passage. Expression of keratin proteins confirmed its epithelial origin. The genetic markers of the RTCC -1/KMC cell line were HLA-A11, B46, B60, Cw1, Cw7, DRw12 and DRw16. The human papillomavirus and herpes simplex virus genomes were not found in this cell line.
Chiang
L C
LC
Department of Microbiology, Kaohsiung Medical College, Taiwan, Republic of China.
Chiang
W
W
Chang
L L
LL
Wu
W J
WJ
Huang
C H
CH
D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D007682:Kidney Pelvis; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D005819:Genetic Markers