Determination of anti-AGE antibodies in human serum.
The aim of this study was to develop an immunoenzyme method for the determination of anti-AGE antibodies in human serum. Human aortic elastin glycated in vitro (AGE-elastin) was used as an antigen, expressing AGE-epitopes, common to all glycated proteins. Polyclonal serum from guinea-pig against AGE-Hemocyanin was obtained according to Nakayama et al. [(1989) Biochem Biophys Res Commun 162: 740-45] and its specificity was tested via direct and competitive ELISA. Sera of 20 type 1 diabetic patients and 20 healthy subjects were tested using the method described. Seventeen patients had elevated levels of competing factors that may be anti-AGE antibodies, compared with the healthy group. The method could be used for investigation of different clinical groups of type 1 diabetic patients. Such a study would help in understanding the pathogenic role of autoantibodies against advanced glycation end products of proteins for the development of long-term diabetic complications.
Baydanoff
S
S
Department of Biology/Immunology, University School of Medicine, Pleven, Bulgaria.
Konova
E
E
Ivanova
N
N
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications / Q000276:immunology*; D004549:Elastin; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D017127:Glycation End Products, Advanced / Q000276:immunology*; D006168:Guinea Pigs; D006433:Hemocyanins; D006801:Humans; D012016:Reference Values
D000906:Antibodies; D001323:Autoantibodies; D000939:Epitopes; D017127:Glycation End Products, Advanced; D004549:Elastin; D006433:Hemocyanins