Regulation of rat granulosa cell alpha-inhibin expression by luteinizing hormone, estradiol, and progesterone.
OBJECTIVE
Our purpose was to assess the effects of follicle-stimulating hormone, luteinizing hormone, forskolin (an adenylyl cyclase activator), estradiol, and progesterone on alpha-inhibin promoter activity in an in vitro fusion gene-transfection system.


METHODS
A fusion gene consisting of the alpha-inhibin 5' flanking and promoter regions linked to the chloramphenicol acetyl transferase reporter gene was constructed. A granulosa cell line originally derived from an inbred strain of Berlin Duckrey rats was transiently transfected with the fusion gene. Fusion gene activity was determined by measuring chloramphenicol acetyl transferase activity in transfected cells.


RESULTS
Both follicle-stimulating hormone and luteinizing hormone activated the alpha-inhibin promoter. Activity in response to combined luteinizing hormone-forskolin treatment was greater than the summation of the activities of the two treatments individually, suggesting that the effects of luteinizing hormone might be partially mediated by second messengers other than cyclic adenosine monophosphate. Gonadotropin-stimulated activity was diminished by estradiol and combined estradiol-progesterone treatments.


CONCLUSIONS
The stimulatory effects of follicle-stimulating hormone and luteinizing hormone on alpha-inhibin production are mediated at least in part by stimulation of the alpha-inhibin promoter. The stimulatory effects are blunted by estradiol-progesterone. These observations may partially explain how alpha-inhibin is down-regulated in vivo in response to the preovulatory luteinizing hormone surge.
Tekmal
R R
RR
Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio 78284-7836, USA.
Burns
W N
WN
Rao
D V
DV
Montoya
I A
IA
Chang
P L
PL
Stoica
G
G
Schenken
R S
RS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D007265:Inhibins; D007986:Luteinizing Hormone / Q000494:pharmacology*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D015335:Molecular Probes; D010455:Peptides; C094377:inhibin-alpha subunit; D005576:Colforsin; D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone