Dependence of solution exchange time on cell or patch linear dimensions in concentration jump experiments using patch-clamped sensory neurones.
A critical evaluation of "concentration jump" techniques employing hydrodynamic solution exchange has been undertaken. Data obtained from experiments using acutely isolated sensory neurones demonstrated a dependence of the total solution exchange time on the linear dimensions of the preparation. The exchange time around a circular surface is proportional to the radius of this surface. A quantitative approximation can be formulated as follows: if the membrane area of the object under investigation is increased n times, the duration of the full solution exchange is increased by radical n times. A nonlinear dependence of exchange time on vacuum pressure was confirmed in a separate set of experiments.
Pidoplichko
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Department of Neurophysiology, Federal Institute for Neurobiology, PO Box 1860, Brenneckestrasse 6, D-39008 Magdeburg, Germany.
D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology; D007700:Kinetics; D009475:Neurons, Afferent / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000502:physiology; D012996:Solutions; D013499:Surface Properties; D012668:Trigeminal Ganglion / Q000166:cytology*
D015222:Sodium Channels; D012996:Solutions; D012964:Sodium; D011188:Potassium