Induction of human monocyte motility by lysyl oxidase.
Lysyl oxidase highly purified from calf aorta was found to be a potent chemotactic agent for unstimulated human peripheral blood mononuclear cells, determined in in vitro assays in Boyden chambers. A typical chemotactic bell-shaped curve was observed, with a maximal migratory response of 237% of control occurring at 10(-10) M lysyl oxidase. The chemotactic response was prevented by prior heat inactivation of the enzyme, by treatment of the enzyme with beta-aminopropionitrile or ethylenediamine, which are active site-directed inhibitors of lysyl oxidase, and by a competing, lysine-containing peptide substrate of lysyl oxidase. The chemoattractant response to lysyl oxidases was characterized by both chemokinetic and chemotactic components. These results raise the possibility that extracellular lysyl oxidase may have important roles to play in biology in addition to its established function in the crosslinking of elastin and collagen.
Lazarus
H M
HM
Pulmonary Center, Boston University School of Medicine, Massachusetts, USA.
Cruikshank
W W
WW
Narasimhan
N
N
Kagan
H M
HM
Center
D M
DM
D000629:Aminopropionitrile / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005029:Ethylenediamines / Q000494:pharmacology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008249:Protein-Lysine 6-Oxidase / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D002630:Chemotactic Factors; D004791:Enzyme Inhibitors; D005029:Ethylenediamines; D000629:Aminopropionitrile; C031234:ethylenediamine; D008249:Protein-Lysine 6-Oxidase