Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies.
The unmasking of estradiol receptor in paraffin sections of Bouin's-fixed uterine tissue from ovariectomized gilts was attained with microwave treatment. Immunocytochemistry of the receptor was performed using a polyclonal or five monoclonal antibodies, two of which are commercially available, reacting with different domains of the protein and an amplified-peroxidase system for detection. With five of the antibodies, a predominance of nuclear staining was observed in cells of endometrial glands, while one monoclonal antibody (13H2), reacting with the receptor's domain E, showed a preference for the cytoplasmic receptor. In stroma, all antibodies detected more receptor in nuclei than in cytoplasm. In epithelium, the commercially available antibody H222, our monoclonals 13H2 and HT65, and the polyclonal antibody 402 demonstrated more receptor in cytoplasmic than in nuclear areas. In myometrium, the nuclei from longitudinal and ring muscles were definitely stained with the antibodies. We conclude that the accessibilities of the antibody epitopes of the receptor differ according to the functional uterine cell type.
Sierralta
W D
WD
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
Thole
H H
HH
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D008872:Microwaves; D016612:Paraffin Embedding; D011959:Receptors, Estradiol / Q000032:analysis*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013552:Swine; D014599:Uterus / Q000737:chemistry*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D011959:Receptors, Estradiol