1-Benzylimidazole induces rat hepatic microsomal epoxide hydrolase with the elevation of its mRNA levels.
The effects of 1-benzylimidazole on the expression of microsomal epoxide hydrolase (mEH) gene were examined in rat. Immunoblot analyses showed that hepatic microsomes isolated from rat treated with 1-benzylimidazole at 25, 50 or 100 mg/kg/day for 3 or 6 days exhibited 2-3-fold increases in mEH levels, relative to control microsomes. Northern and slot blot analysis revealed that 1-benzylimidazole is effective in increasing hepatic mEH mRNA levels at 24 h post-treatment (i.e. 4-5-fold). Hepatic mEH mRNA levels were elevated from 4-4- to 8-fold following 1-benzylimidazole administration in a time-dependent manner. These results demonstrated that 1-benzylimidazole induces mEH protein in rat and that the induction is primarily associated with accumulation of mEH mRNA levels.
Cho
J Y
JY
College of Pharmacy, Duksung Women's University, Seoul, Korea.
Nam
S Y
SY
Kim
S G
SG
D000818:Animals; D015152:Blotting, Northern; D004790:Enzyme Induction; D004851:Epoxide Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D007093:Imidazoles / Q000494:pharmacology*; D015151:Immunoblotting; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D007093:Imidazoles; D012333:RNA, Messenger; C017062:1-benzylimidazole; D004851:Epoxide Hydrolases