Determination of schizandrin in human plasma by gas chromatography-mass spectrometry.
Schizandrin (SZ) is one of the lignan components from Schisandra fruits. A highly sensitive and precise method for the determination of SZ in human plasma was developed involving selected-ion monitoring with gas chromatography-mass spectrometry using a fused-silica capillary column. A 0.1-ml plasma sample was used for solid-phase extraction. A good linear relationship was obtained in the concentration range studied (2.0-500 ng/ml) and the method was sufficiently accurate and precise to support clinical pharmacokinetic studies. After oral administration of SZ at a dose of 15 mg to healthy male subjects, the average value of the maximum plasma concentration of SZ was 96.1 +/- 14.1 ng/ml. The plasma concentration of this substance could be monitored for 8 h after administration.
Ono
H
H
Tsumura Central Research Laboratories, Ibaraki, Japan.
Matsuzaki
Y
Y
Wakui
Y
Y
Takeda
S
S
Ikeya
Y
Y
Amagaya
S
S
Maruno
M
M
D034242:Cyclooctanes; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D017705:Lignans / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D010946:Plants, Medicinal; D011083:Polycyclic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C011105:schizandrin