Simultaneous reversed-phase high-performance liquid chromatographic separation of non-polar isoprenoid lipids and their determination.
A procedure for the rapid identification and determination of non-polar isoprenoid lipids from animal tissues was developed. The complete determination can be carried out by reversed-phase HPLC of just two samples. The first, extracted from unaltered tissues and suitably processed by column chromatography, provides information about free cholesterol, cholesteryl esters, coenzymes Q, free dolichols and dolichyl esters. The second, obtained from saponified tissues, can be used to detect both total cholesterol and total dolichols. Specific calibration graphs were constructed for the determination of the different constituents.
Piretti
M V
MV
Dipartimento di Biochimica, Università di Bologna, Italy.
Pagliuca
G
G
Tarozzi
G
G
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002784:Cholesterol / Q000302:isolation & purification; D002788:Cholesterol Esters / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004286:Dolichols / Q000302:isolation & purification*; D008055:Lipids / Q000302:isolation & purification*; D051381:Rats; D013185:Squalene / Q000302:isolation & purification; D014280:Triglycerides / Q000302:isolation & purification; D014451:Ubiquinone / Q000302:isolation & purification
D002788:Cholesterol Esters; D004286:Dolichols; D008055:Lipids; D014280:Triglycerides; D014451:Ubiquinone; D013185:Squalene; D002784:Cholesterol