Direct-rapid (DR) mutagenesis of large plasmids using PCR.
Gatlin
J
J
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425-2230, USA.
Campbell
L H
LH
Schmidt
M G
MG
Arrigo
S J
SJ
D000251:Adenosine Triphosphatases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D039102:Poly(A)-Binding Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D017385:Sequence Homology; D014162:Transfection
D001426:Bacterial Proteins; D017931:DNA Primers; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins