Clonal diversity of oral Eikenella corrodens within individual subjects by arbitrarily primed PCR.
The genetic diversity of 205 Eikenella corrodens isolates recovered from dental plaque, mucosal surfaces, and saliva of 24 subjects was examined by arbitrarily primed PCR. Twenty-two subjects were colonized by multiple clones (range, two to eight; mean, 3.7). This study demonstrates the utility of arbitrarily primed PCR for clonal analysis of E. corrodens and the multiclonal colonization of E. corrodens in the oral cavity.
Chen
C
C
University of Southern California School of Dentistry, Department of Periodontology, Los Angeles 90089, USA. cochcn@hsc.usc.edu
Ashimoto
A
A
D000328:Adult; D010520:Aggressive Periodontitis / Q000382:microbiology; D001483:Base Sequence; D002648:Child; D017931:DNA Primers / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology; D016124:Eikenella corrodens / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014644:Genetic Variation; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D009061:Mouth Mucosa / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000382:microbiology
D017931:DNA Primers