Microwave-accelerated direct immunofluorescent staining for respiratory syncytial virus and influenza A virus.
A microwave-accelerated direct immunofluorescence staining method which requires only 20 min from specimen receipt to interpretation is as effective as conventional methods for detecting respiratory syncytial virus and influenza A virus antigens in clinical specimens. The time required compares favorably with that for the less sensitive Abbott Test Pack RSV.
Hite
S A
SA
Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, Ohio 44106, USA.
Huang
Y T
YT
D000956:Antigens, Viral / Q000032:analysis; D005069:Evaluation Studies as Topic; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D008872:Microwaves; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D000956:Antigens, Viral