title: Role of intercellular adhesion molecule-1 in antigen-induced lung inflammation in brown Norway rats.
abstract: We investigated the involvement of intercellular adhesion molecule-1 (ICAM-1; CD54) in ovalbumin (OA) antigen-induced lung inflammation in sensitized Brown Norway (BN) rats by using flow cytometry and in vivo treatment with a murine monoclonal antibody (MAb), 1A29, directed against rat ICAM-1. OA-challenge induced an eosinophil and lymphocyte-rich accumulation of leukocytes into the airway lumen. Between 75 and 90% of the T cells in bronchoalveolar lavage (BAL) fluid after challenge expressed CD54 and CD11a and were of the memory phenotype. 1A29 treatment produced dose-related increases in circulating 1A29 and blood neutrophils. In the BAL fluid of 1A29-treated animals, significant (P < 0.05) reductions in the numbers of eosinophils and lymphocytes, but not neutrophils or alveolar macrophages, were observed in association with a reduced inflammatory pathology in lung tissue. 1A29 administration reduced the number of detectable ICAM-1 binding sites on T cells in peripheral blood and BAL fluid examined ex vivo by flow cytometry. We conclude that ICAM-1 is critically important for the antigen-specific recruitment of eosinophils and lymphocytes into the lungs.
authors.affiliation: Pharmacia and Upjohn, Inc., Kalamazoo, Michigan 49001, USA.
authors.forename: I M
authors.initials: IM
authors.lastname: Richards
authors.forename: K P
authors.initials: KP
authors.lastname: Kolbasa
authors.forename: G E
authors.initials: GE
authors.lastname: Winterrowd
authors.forename: C A
authors.initials: CA
authors.lastname: Hatfield
authors.forename: S L
authors.initials: SL
authors.lastname: Vonderfecht
authors.forename: S F
authors.initials: SF
authors.lastname: Fidler
authors.forename: R L
authors.initials: RL
authors.lastname: Griffin
authors.forename: J R
authors.initials: JR
authors.lastname: Brashler
authors.forename: R F
authors.initials: RF
authors.lastname: Krzesicki
authors.forename: C L
authors.initials: CL
authors.lastname: Lane
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: L M
authors.initials: LM
authors.lastname: Sly
authors.forename: N D
authors.initials: ND
authors.lastname: Staite
authors.forename: J E
authors.initials: JE
authors.lastname: Chin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D001773:Blood Cells / Q000502:physiology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D007114:Immunization; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008221:Lymphoid Tissue / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D010641:Phenotype; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011914:Rats, Inbred BN; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin