Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1.
To study the origin of interleukin 1 (IL-1) present in human follicular fluid we determined the percentage of macrophages (MO) and cells with HLA-DR antigen (DR+) present in 22 samples of human follicular fluid (FF) from women undergoing in vitro fertilization, and examined the release of IL-1 beta by cultures of purified human granulosa cells (GC). The number of red blood cells (RBC) in the crude preparation was taken as a measure of possible contamination with peripheral blood monocytes (assuming a ratio of one monocyte or MO per 10(4) RBC). For the evaluation of MO and DR+ cells percentages we employed an indirect immunofluorescence technique using specific monoclonal antibodies. Total cells from FF were purified by Ficoll-Hypaque density gradient centrifugation (delta = 1.076 g/l and GC were purified using a gradient delta = 1.065 g/l. This method reduced the contamination with MO to 0-1%. The spontaneous release of IL-1 beta was measured by ELISA. We found that FF contained 9.81 +/- 1.47% of MO but only 7.85% were ovarian MO. In addition the total percentage of DR+ cells was 17.13 +/- 2.35% but only 9.81% corresponded to MO. Therefore about 7.32% of DR+ cells could be GC. Then purified GC (10(4)/0.2 ml/well) were cultured during 24 hours at 37 degrees C in serum free medium (DMEM:F12). IL-1 beta levels were 84 +/- 17 pg/ml and these values were increased by 44% when GC were stimulated with FSH (200 ng/ml). These results suggest that GC produced IL-1 beta and that the synthesis of this cytokine might be regulated by hormones.
Barañao
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RI
Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina.
Dáin
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Palak de Fried
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Rumi
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D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000166:cytology; D006107:Granulosa Cells / Q000276:immunology* / Q000378:metabolism; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008264:Macrophages
D006684:HLA-DR Antigens; D007375:Interleukin-1; D005640:Follicle Stimulating Hormone