Synthesis of tumor necrosis factor-alpha mRNA in bronchoalveolar lavage cells from human immunodeficiency virus-infected persons with Pneumocystis carinii pneumonia.
Bronchoalveolar lavage fluid cells from a cohort of 34 human immunodeficiency virus-infected persons with established Pneumocystis carinii pneumonia were examined for expression of tumor necrosis factors (TNF-alpha) mRNA by fluorescence in situ hybridization with an antisense riboprobe. Video image analysis was used to develop a quantitative assay that evaluates relative single-cell levels of mRNA. The resulting data were analyzed as an antisense-to-sense ratio and examined for correlation between TNF-alpha mRNA expression and other measures of disease severity. Higher levels of TNF-alpha mRNA were seen in persons who had higher levels of arterial oxygen.
Rayment
N
N
Department of Immunology, Division of Pathology and Infectious Diseases, University College London Medical School, United Kingdom.
Miller
R F
RF
Ali
N
N
Binks
M H
MH
Katz
D R
DR
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000276:immunology* / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D011020:Pneumonia, Pneumocystis / Q000276:immunology*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D016372:RNA, Antisense; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha