A comparison between solid phase extraction and supercritical fluid extraction for the determination of fluconazole from animal feed.
The application of supercritical fluid extraction with carbon dioxide and modified carbon dioxide for the determination of fluconazole from an animal feed was studied. A fractional factorial design approach was used to examine the significant experimental variables for quantitative extraction of fluconazole. Gas chromatography with either flame ionisation or mass selective detection was used for quantitation of the extracts. The results indicated that modifier (methanol) had the greatest effect on the recovery of fluconazole from the animal feed.
Khundker
S
S
Department of Chemical and Life Sciences, University of Northumbria at Newcastle, Newcastle upon Tyne, UK.
Dean
J R
JR
Jones
P
P
D000821:Animal Feed / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D015725:Fluconazole / Q000032:analysis* / Q000302:isolation & purification; D007202:Indicators and Reagents; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D007202:Indicators and Reagents; D015725:Fluconazole