title: Interleukin-6 secretion in vitro is up-regulated in ectopic and eutopic endometrial stromal cells from women with endometriosis.
abstract: An in vitro model developed to compare human endometrial and endometriosis stromal cells was used to examine basal and stimulated expression of interleukin (IL-6). Stromal cells isolated from normal endometrium (NE) exhibited the lowest level of IL-6 secretion (84 pg/10(6) cells-48 h), whereas those cells isolated from endometriosis implants (EI) secreted the highest concentration of this inflammatory cytokine (46,284 pg/10(5) cells-48 h; P < 0.01). Eutopic endometrial stromal cells from women with endometriosis (EE) expressed an intermediate concentration of IL-6 (831 pg/10(6) cells-48 h). Stimulation of the various cultures with IL-1 beta dramatically augmented stromal cell production of IL-6. The mean concentrations of stimulated IL-6 secretion were 16,257, 37,800, and 264,290 pg/10(5) cells-48 h for NE, EE, and EI cells, respectively (P < 0.03). Exposure of the cell cultures to 10 nmol/L estradiol had little direct effect on IL-6 production. The results indicate that endometrial stromal cells isolated from tissues of women with and without endometriosis express IL-6 under basal and cytokine-stimulated conditions. Differential responsiveness among the three cell sources indicates that NE, EE, and EI cells have intrinsic quantitative differences in cytokine regulation.
authors.affiliation: Division of Reproductive Endocrinology, University of California, San Francisco 94143, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Tseng
authors.forename: I P
authors.initials: IP
authors.lastname: Ryan
authors.forename: T D
authors.initials: TD
authors.lastname: Milam
authors.forename: J T
authors.initials: JT
authors.lastname: Murai
authors.forename: E D
authors.initials: ED
authors.lastname: Schriock
authors.forename: D V
authors.initials: DV
authors.lastname: Landers
authors.forename: R N
authors.initials: RN
authors.lastname: Taylor
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism*; D009994:Osmolar Concentration; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D004958:Estradiol