title: Escherichia coli O157:H7 in microbial flora of sheep.
abstract: We found naturally occurring, potentially virulent Escherichia coli O157:H7 strains in sheep. The incidence of E. coli O157:H7 was transient and ranged from 31% of sheep in June to none in November. The use of a sensitive culture technique and the choice of the proper sampling season were both essential for detecting this bacterium in sheep. DNA hybridizations showed that 80% of the E. coli O157:H7 isolates had at least two of the Shiga-like toxin types I or II or the attaching-effacing lesion genes.
authors.affiliation: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow 83843, USA.
authors.forename: I T
authors.initials: IT
authors.lastname: Kudva
authors.forename: P G
authors.initials: PG
authors.lastname: Hatfield
authors.forename: C J
authors.initials: CJ
authors.lastname: Hovde
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D002417:Cattle; D015342:DNA Probes; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D005798:Genes, Bacterial; D008460:Meat / Q000382:microbiology; D012621:Seasons; D012756:Sheep / Q000382:microbiology*; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014774:Virulence / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2