Basic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum.
The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow substrate specificity. Within the external pH range of 5.5 to 7.5, the transmembrane electrical potential (delta psi) functions as the main driving force for uphill transport of arginine, although a low level of uptake was observed when only a transmembrane pH gradient was present. It is concluded that the basic amino acid permease is a H+ symporter. Quantitative analysis of the steady-state levels of arginine uptake in relation to the proton motive force suggests a H+-arginine symport stoichiometry of one to one. Efflux studies demonstrated that the basic amino acid permease functions in a reversible manner.
Hillenga
D J
DJ
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
Versantvoort
H J
HJ
Driessen
A J
AJ
Konings
W N
WN
D026905:Amino Acid Transport Systems; D001120:Arginine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008564:Membrane Potentials; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*; D010806:Physical Education and Training
D026905:Amino Acid Transport Systems; D003574:Cytochrome c Group; D007476:Ionophores; D008081:Liposomes; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D001120:Arginine; D008239:Lysine