Serotonin uptake and its kinetics in platelets of women with Alzheimer's disease.
The uptake of serotonin (14C-5-hydroxytryptamine, 5HT) in platelets and its kinetic characteristics were investigated in a group of women (n = 20) with probable Alzheimer's Disease (mean age = 76.0, years, SD = 8.27, range = 63-88) and in healthy normal women (n = 18, mean age = 72.6 years, SD = 7.24, range = 61-84). Both the apparent affinity of binding of 5HT to the platelet membrane (Km) and the maximum velocity (Vmax) of the rate of transport of 14C-5HT into platelets were significantly higher in the Alzheimer's Disease group than in the normal comparison group. Initial velocity of 14C-5HT uptake not passive diffusion (nonspecific uptake of 14C-5HT at 4 degrees C) differed significantly in the two groups. Our findings suggest abnormalities in the kinetic mechanisms involved in the uptake of 14C-5HT by platelets in women with Alzheimer's Disease.
Kumar
A M
AM
Department of Psychiatry and Behavioral Sciences, University of Miami School of Medicine, FL 33101, USA.
Kumar
M
M
Sevush
S
S
Ruiz
J
J
Eisdorfer
C
C
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis; D001792:Blood Platelets / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D008609:Mental Status Schedule; D008875:Middle Aged; D012016:Reference Values; D012701:Serotonin / Q000097:blood*
D012701:Serotonin