Transforming growth factor-alpha and epidermal growth factor in hamster tissues: biochemical and immunohistochemical studies.
In Syrian golden hamster kidneys and submaxillary glands, the levels of EGF, determined by radioimmunoassay, were much lower than in the same organs of two other rodent species, mouse and rat. In submaxillary glands, the EGF/TGF-alpha receptor-binding activities were also much lower in hamster than in mouse and rat. In contrast, the TGF-alpha content of hamster kidneys, determined by radioimmunoassay, was higher than in the kidneys of the other animals, as was the EGF/TGF-alpha receptor-binding activity. Using immunohistochemistry, the TGF-alpha immunoreactivity in hamster kidneys was localized both in proximal and distal tubules with the exception of the macula densa area. The levels of TGF-alpha in the submaxillary glands were very low in all the animals tested. Hamster kidney extracts contained a specific immunoreactive protein with the M(r) and the N-terminal amino acid sequence (VVSHFNECPD) expected for mature hamster TGF-alpha. Western blot analysis of hamster renal solubilized membrane proteins using anti-EGF receptor antibodies revealed three immunoreactive protein bands of which one had the M(r) expected for the EGF/TGF-alpha receptor. The immunohistochemical pattern of this receptor in hamster kidneys proximal tubular cells was very similar to the other tested rodent species.
Journé
F
F
Department of Biological Chemistry, University of Mons-Hainaut, Belgium.
Wattiez
R
R
Severyns
C
C
Nonclercq
D
D
Toubeau
G
G
Heuson-Stiennon
J A
JA
Falmagne
P
P
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000033:anatomy & histology / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
D011506:Proteins; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D066246:ErbB Receptors