title: Characterization of hematopoietic cells arising on the textured surface of left ventricular assist devices.
abstract: BACKGROUND
  Textured biomaterial surfaces in implantable left ventricular assist devices induce development of a nonthrombotic neointimal surface and allow elimination of anticoagulation therapy in device recipients. Characterization of the hematopoietic cells formed within the neointimal surfaces of these devices will contribute to our understanding of this unique neointima.
  
  
  METHODS
  The blood-contacting surface of seven ThermoCardiosystems left ventricular assist devices was removed, washed with phosphate-buffered saline solution, and digested with 0.1% collagenase for 15 to 20 minutes. The hematopoietic cells released from the explants were isolated and analyzed by flow cytometry and immuno-histochemical staining.
  
  
  RESULTS
  More than 80% +/- 6% of hematopoietic cells isolated in this fashion are of myelomonocytic origin and express CD14, CD15, and CD33 surface molecules. Four percent of cells express the CD34 surface marker, which suggests that the neointima is colonized by pluripotent hematopoietic stem cells. Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin-3, c-kit ligand, granulocyte colony-stimulating factor resulted in tenfold expansion by day 7 and 25-fold expansion by day 14.
  
  
  CONCLUSIONS
  Pluripotent hematopoietic cells with a high proliferative capacity colonize textured surfaces of left ventricular assist devices and may contribute to the development of a biologically nonthrombogenic neointima.
authors.affiliation: Department of Hematology-Oncology, Cornell University Medical Center, New York, New York, USA.
authors.forename: S
authors.initials: S
authors.lastname: Rafii
authors.forename: M C
authors.initials: MC
authors.lastname: Oz
authors.forename: J A
authors.initials: JA
authors.lastname: Seldomridge
authors.forename: B
authors.initials: B
authors.lastname: Ferris
authors.forename: A S
authors.initials: AS
authors.lastname: Asch
authors.forename: R L
authors.initials: RL
authors.lastname: Nachman
authors.forename: F
authors.initials: F
authors.lastname: Shapiro
authors.forename: E A
authors.initials: EA
authors.lastname: Rose
authors.forename: H R
authors.initials: HR
authors.lastname: Levin
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006353:Heart-Assist Devices; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013499:Surface Properties
chemical_list: D015703:Antigens, CD; D016207:Cytokines