Effects of morphine on human nasal cilia beat frequency in vitro.
Using human nasal cytological brushings, we have investigated the effects of morphine on ciliary function by measurement of cilia beat frequency in vitro, and we have also determined opioid receptor binding in these specimens. We obtained ciliated samples from seven volunteers, and measured cilia beat frequency using the transmitted light technique during exposure to morphine 10 mumol litre-1 for 4 h. Mean cilia beat frequency of the samples exposed to morphine was 11.1 (95% confidence interval 10.9-11.5) Hz and that of the controls 11.3 (11.1-11.7) Hz. There was no significant effect of morphine on human cilia beat frequency in vitro (MANOVA for repeated measures and nested, F = 0.61, P = 0.66). In a separate study, we obtained nasal brushings from 20 patients and measured the binding of the opioid antagonist tritiated diprenorphine ([3H]DPN). Mean disintegrations per minute (dpm) for total and non-specific binding were 9036 (8105-9967) dpm and 9130 (8054-10206) dpm, respectively. These values did not differ significantly (paired t test, t = 0.22, P = 0.83). We conclude that morphine had no effect on cilia beat frequency in vitro and we were unable to demonstrate any significant numbers of opioid receptors on nasal ciliated epithelium.
Selwyn
D A
DA
University Department of Anaesthesia, Leicester Royal Infirmary.
Raphael
J H
JH
Lambert
D G
DG
Langton
J A
JA
D000328:Adult; D000701:Analgesics, Opioid / Q000494:pharmacology*; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009020:Morphine / Q000494:pharmacology*; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism; D011957:Receptors, Opioid / Q000378:metabolism
D000701:Analgesics, Opioid; D011957:Receptors, Opioid; D009020:Morphine