title: Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre-activated ferric species.
abstract: Nitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time-dependent shortening of the kinetic lag phase.
authors.affiliation: Institute of Biochemistry, University Clinics Charité, Humboldt University, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Wiesner
authors.forename: J
authors.initials: J
authors.lastname: Rathmann
authors.forename: H G
authors.initials: HG
authors.lastname: Holzhütter
authors.forename: R
authors.initials: R
authors.lastname: Stösser
authors.forename: K
authors.initials: K
authors.lastname: Mäder
authors.forename: H
authors.initials: H
authors.lastname: Nolting
authors.forename: H
authors.initials: H
authors.lastname: Kühn
mesh_terms: D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation; D005290:Ferric Compounds / Q000378:metabolism*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D013057:Spectrum Analysis
chemical_list: D005290:Ferric Compounds; D008041:Linoleic Acids; D016859:Lipoxygenase Inhibitors; D009569:Nitric Oxide; D019787:Linoleic Acid; D001093:Arachidonate 15-Lipoxygenase