title: Autoradiographic studies on the uptake of 3H-noradrenaline and 3H-serotonin by neurones and astrocytes in explant and primary cultures of rat CNS: effects of antidepressants.
abstract: Autoradiographic studies were made on the uptake of 3H-noradrenaline and 3H-serotonin in explant cultures and primary astrocyte cultures from various regions of rat central nervous system (cortex, cerebellum, locus coeruleus, nucleus raphé, spinal cord). In explant cultures from locus coeruleus and nucleus raphé cell bodies and processes of many neurones revealed intense labelling by 3H-noradrenaline and 3H-serotonin, respectively. In cultures from cortex, cerebellum and spinal cord the cell bodies of neurones did not show labelling by the monoamines but many nerve fibres in the outgrowth zone had taken up 3H-noradrenaline and 3H-serotonin. Astrocytes in explant cultures did not take up 3H-noradrenaline and 3H-serotonin whereas astrocytes in primary cultures showed heavy uptake of both monoamines. In contrast, amino acid transmitters such as 3H-GABA and 3H-glutamate were accumulated by astrocytes in explant as well as in primary cultures. Uptake of both 3H-noradrenaline and 3H-serotonin by neurones and astrocytes was considerably reduced or inhibited in Na(+)-free incubation medium or at low temperature, suggesting an active uptake mechanism. Addition of the antidepressants maprotiline and (+)oxaprotiline inhibited the uptake of 3H-noradrenaline by neuronal cell bodies and fibres in explant cultures and by astrocytes in primary cultures. The uptake of 3H-serotonin by neurones and astrocytes was blocked by citalopram and paroxetine. Our studies demonstrate that astrocytes in primary cultures are able to actively take up 3H-noradrenaline and 3H-serotonin whereas there was no uptake of monoamines into astrocytes in explant cultures, suggesting that there is a difference between astrocytes in different culture systems (explant cultures vs primary cultures) with respect to the uptake of monoamine transmitters.
authors.affiliation: Department of Physiology, University of Basel, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Hösli
authors.forename: L
authors.initials: L
authors.lastname: Hösli
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D001345:Autoradiography; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D046508:Culture Techniques; D018698:Glutamic Acid / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000928:Antidepressive Agents; D016895:Culture Media, Serum-Free; D018698:Glutamic Acid; D009638:Norepinephrine; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D005680:gamma-Aminobutyric Acid