Isolation and characterization of a sialo-glycopeptide from buffalo colostrum.
A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic acid in the ratio 1:2:3:4:1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry. 1H-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type.
Aparna
H S
HS
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
Salimath
P V
PV
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002020:Buffaloes; D002240:Carbohydrate Sequence; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011247:Pregnancy; D012795:Sialoglycoproteins / Q000737:chemistry / Q000302:isolation & purification*
D000596:Amino Acids; D012795:Sialoglycoproteins