title: Molecular cloning of a novel human cDNA, RT14, containing a putative ORF highly conserved between human, fruit fly, and nematode.
abstract: From a human fetal-brain cDNA library we isolated a novel gene, designated RT14, whose cDNA contained an open reading frame of 375 nucleotides encoding 125 amino acids. A 71-amino-acids region of the predicted peptide sequence showed 62% identity with a putative peptide encoded in the region close to Drosophila Tra-2. It also showed significant homology with the putative peptide of T20G5.10 of C. elegans. Northern-blot analysis revealed expression of 0.7-kb and 1.1-kb transcripts in all human tissues examined.
authors.affiliation: Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: T K
authors.initials: TK
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: H
authors.initials: H
authors.lastname: Shinomiya
authors.forename: Y
authors.initials: Y
authors.lastname: Kuga
authors.forename: H
authors.initials: H
authors.lastname: Hishigaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009348:Nematoda / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: C104378:BLOC1S1 protein, human; D009419:Nerve Tissue Proteins; D004247:DNA