title: Virus association with lymphocytes in acute African swine fever.
abstract: This paper reports the presence of mature viral particles within the lymphocytes of samples taken from pigs inoculated with a highly virulent African swine fever (ASF) virus isolate (Malawi 83), and the adhesion of the lymphocytes to macrophages containing the virus replication sites. Virus replication in lymph-node medullar tissue macrophages was observed from 3 days post inoculation (pi). At 3 days pi, transmission electron microscopy revealed hemadsorption in some infected macrophages. At 5 and 7 days pi, a number of macrophages with virus replication were surrounded by a ring of lymphocytes. In such cases, mature viral particles were observed in membrane evaginations of the infected cell that corresponded to invaginations of the lymphocyte membrane. Also at 5 and 7 days pi, mature virions were observed within the cytoplasm of some lymphocytes. However, incomplete virions and African swine fever virus replication sites were not observed within the lymphocytes.
authors.affiliation: Departamento de Anatomía, Facultad de Veterinaria, Universidad de Cordoba, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: F C
authors.initials: FC
authors.lastname: de Lara
authors.forename: J
authors.initials: J
authors.lastname: Martín de las Mulas
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: J
authors.initials: J
authors.lastname: Hervás
authors.forename: P J
authors.initials: PJ
authors.lastname: Wilkinson
authors.forename: M A
authors.initials: MA
authors.lastname: Sierra
mesh_terms: D000357:African Swine Fever / Q000276:immunology* / Q000473:pathology / Q000821:virology; D000358:African Swine Fever Virus / Q000302:isolation & purification* / Q000502:physiology*; D000818:Animals; D005260:Female; D008198:Lymph Nodes / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D008214:Lymphocytes / Q000821:virology*; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D008295:Malawi; D008297:Male; D013552:Swine; D013997:Time Factors; D014779:Virus Replication