DNA immunization induces protective immunity against B-cell lymphoma.
Idiotypic determinants of the immunoglobulin expressed on the surface of B-cell lymphomas are tumor-specific antigens (TSAs), which can be targeted by immunotherapy. Immunization with DNA constructs encoding the idiotype (ld) of a murine B-cell lymphoma induced specific anti-ld antibody responses and protected mice against tumor challenge. Use of DNA encoding an ld/GM-CSF (idiotype/granulocyte-macrophage colony-stimulating factor) fusion protein improved vaccine efficacy, and xenogeneic immunoglobulin constant region determinants were required for immunogenicity. These results indicate that DNA may be a simple and efficacious means of inducing immune responses against a weak, otherwise unrecognized tumor antigen, provided that additional stimuli are included with the DNA.
Syrengelas
A D
AD
Department of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford University Medical Center, California 94305-5306, USA.
Chen
T T
TT
Levy
R
R
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008809:Mice, Inbred C3H; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014407:Tumor Cells, Cultured; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000951:Antigens, Neoplasm; D007130:Immunoglobulin Idiotypes; D011993:Recombinant Fusion Proteins; D014612:Vaccines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA