[Development of a device allowing for the perfusion and preservation of rat kidneys at -4 degrees C].
OBJECTIVE
In order to prolong the cold ischaemia time and to improve the quality of donor kidneys, we have designed and developed a renal perfusion machine allowing the control of perfusion parameters (temperature, pressure, flow rate, resistance) during the various phases of a kidney perfusion and storage protocol at -4 degrees C.


METHODS
Twenty four rat kidneys were removed and the effects of perfusion and storage at -4 degrees C were studied using a perfusion/storage machine allowing the controlled addition of 2,3 butanediol in University of Wisconsin (UW solution). The kidneys were stored for 96 hours at -4 degrees C and were studied in terms of perfusion parameters (pressure, resistance) and according to their histological appearance.


RESULTS
The machine allows controlled perfusion of a cryoprotective agent and preservation of kidneys at -4 degrees C for 96 hours.


CONCLUSIONS
In animals, it is possible to store kidneys at a temperature of -4 degrees C for 96 hours by using a vecor solution (UW solution) and a cryoprotective agent (2,3 butanediol). The perfusion and storage of organs under these conditions must be performed by a computer-assisted machine, allowing monitoring and control of the various steps of perfusion/storage.
Eschewege
P
P
Laboratoire de Chirurgie Expérimentale et Service d'Urologie, Faculté de Médecine Paris-Sud, Hôpital de Bicêtre, Le Kremlin Bicêtre.
Godfrin
Y
Y
Conti
M
M
Quillard
J
J
Douguet
D
D
Benoit
G
G
D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation*; D004867:Equipment Design; D007668:Kidney; D008297:Male; D009926:Organ Preservation / Q000295:instrumentation*; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley
Mise au point d'une machine permettant la perfusion et la conservation des reins de rat à -4 degrees C.