Quantitative in vivo measurements using microdialysis on-line with capillary zone electrophoresis.
A system which couples microdialysis with capillary zone electrophoresis (CZE) on-line is used to monitor ascorbate and lactate in the caudate nucleus of rat brain. On-line interface of microdialysis probe and electrophoresis capillary, along with the high mass sensitivity of CZE, allows the probe to be operated at flow rates as low as 40 nl/min. Under these conditions, the relative recovery is nearly 100% and quantitative monitoring is possible. The microscale system also facilitates calibration by the low flow rate method. In spite of the low flow rate, temporal resolution in the 45-125 s range is possible for these compounds. The system is demonstrated by observing changes in ascorbate due to infusions of elevated K+ through the dialysis probe and systemic injections of amphetamine and an anesthetic (ketamine/xylazine/acepromazine mixture). Lactate is monitored in response to elevated K+ infusions.
Lada
M W
MW
Department of Chemistry, University of Florida, Gainesville 32611, USA.
Kennedy
R T
RT
D000818:Animals; D001205:Ascorbic Acid / Q000493:pharmacokinetics; D001921:Brain / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D007773:Lactates / Q000493:pharmacokinetics; D019344:Lactic Acid; D008297:Male; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D007773:Lactates; D019344:Lactic Acid; D001205:Ascorbic Acid