Virus association with lymphocytes in acute African swine fever.
This paper reports the presence of mature viral particles within the lymphocytes of samples taken from pigs inoculated with a highly virulent African swine fever (ASF) virus isolate (Malawi 83), and the adhesion of the lymphocytes to macrophages containing the virus replication sites. Virus replication in lymph-node medullar tissue macrophages was observed from 3 days post inoculation (pi). At 3 days pi, transmission electron microscopy revealed hemadsorption in some infected macrophages. At 5 and 7 days pi, a number of macrophages with virus replication were surrounded by a ring of lymphocytes. In such cases, mature viral particles were observed in membrane evaginations of the infected cell that corresponded to invaginations of the lymphocyte membrane. Also at 5 and 7 days pi, mature virions were observed within the cytoplasm of some lymphocytes. However, incomplete virions and African swine fever virus replication sites were not observed within the lymphocytes.
Carrasco
L
L
Departamento de Anatomía, Facultad de Veterinaria, Universidad de Cordoba, Spain.
de Lara
F C
FC
Martín de las Mulas
J
J
Gómez-Villamandos
J C
JC
Hervás
J
J
Wilkinson
P J
PJ
Sierra
M A
MA
D000357:African Swine Fever / Q000276:immunology* / Q000473:pathology / Q000821:virology; D000358:African Swine Fever Virus / Q000302:isolation & purification* / Q000502:physiology*; D000818:Animals; D005260:Female; D008198:Lymph Nodes / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D008214:Lymphocytes / Q000821:virology*; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D008295:Malawi; D008297:Male; D013552:Swine; D013997:Time Factors; D014779:Virus Replication