Identification of a decapeptide with the binding reactivity for tumor-associated TAG72 antigen from a phage displayed library.
Peptide ligands for tumor-associated TAG72 antigen were identified by screening a large, diverse decapeptide library expressed on the surface of filamentous phages. Fifty-eight clones of phages were selected from the eluates after the third round of biopanning and their DNA inserts were sequenced. A dominant decapeptide HYVSIELPDH (14/58) was found with the binding reactivity for TAG72 antigen in the TAG72-binding ELISA and Western dot blotting. It also showed a preferential binding to colonic adenocarcinomatous cells expressing the TAG72 antigen in the histochemical study. Therefore, this anti-TAG72 decapeptide may be useful in serving as the starting point with regard to further designing peptidomimetics for potential pharmaceuticals.
Gui
J
J
Department of Microbiology, Saskatoon Cancer Center, University of Saskatchewan, Canada.
Moyana
T
T
Malcolm
B
B
Xiang
J
J
D000230:Adenocarcinoma / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001435:Bacteriophages / Q000235:genetics; D001665:Binding Sites; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D006023:Glycoproteins; D009842:Oligopeptides; C047052:tumor-associated antigen 72