DNA copy number losses in chromosome 14: an early change in gastrointestinal stromal tumors.
The DNA copy number changes were investigated in 13 malignant, 3 borderline, and 16 benign gastrointestinal stromal tumors (GISTs) by comparative genomic hybridization. A consistent finding was the loss of DNA copy numbers in chromosome 14q. This was detected in 75% of the benign tumors, in 62% of the malignant tumors, and in two out of the three borderline tumors with a minimal overlapping region located to 14q22. Losses of DNA copy numbers were more frequent than gains, with 2-10 changes in malignant GISTs and 1-3 changes in benign tumors. High-level DNA amplifications, detected only in malignant GISTs, were assigned to 3q26-q29 (40%), 5p (30%), and 8q22-24 (40%). Our results indicate that copy number losses in 14q are an early change during oncogenesis of GISTs and suggest the possibility that a new tumor suppressor gene at 14q22 might be involved in the regulation of differentiation or proliferation of such mesenchymal cells.
el-Rifai
W
W
Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
Sarlomo-Rikala
M
M
Miettinen
M
M
Knuutila
S
S
Andersson
L C
LC
D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008648:Mesoderm; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D017384:Sequence Deletion
D004273:DNA, Neoplasm