title: Rapid and specific precolumn extraction high-performance liquid chromatographic assay for bupivacaine in human serum.
abstract: A rapid and specific HPLC assay for quantiative determination of bupivacaine in human serum is described. The technique incorporates an on-line sample clean-up system followed by reversed-phase chromatography with UV detection. The proposed method uses a column-switching technique and protein-coated Lichrosorb RP-8 as a precolumn together with Lichrosorb RP-18 as an analytical column. The total run time for an injection of serum sample was 10 min. This procedure offers a sensitive assay without the need for time-consuming extractions. The average bupivacaine recoveries over a concentration range of 150-600 ng/mL ranged from 99.12 to 101.02%, and relative standard deviations ranged from 1.15 to 1.78%.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Assiut University, Egypt.
authors.forename: S
authors.initials: S
authors.lastname: Emara
authors.forename: A
authors.initials: A
authors.lastname: Khedr
authors.forename: H
authors.initials: H
authors.lastname: Askal
mesh_terms: D000327:Adsorption; D000704:Analysis of Variance; D000779:Anesthetics, Local / Q000097:blood* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002045:Bupivacaine / Q000097:blood* / Q000378:metabolism; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation*; D006801:Humans; D016014:Linear Models; D009862:Online Systems; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D000779:Anesthetics, Local; D001798:Blood Proteins; D002045:Bupivacaine