Nitric oxide enhances cytotoxicity of cultured rabbit gastric mucosal cells induced by hydrogen peroxide.
While NO has been reported to act as a protective factor to gastric mucosa, it has been shown to be cytotoxic to various cells. NO also has been demonstrated to stimulate prostaglandin (PG) release and mucous glycoprotein secretion which could result in the activation of gastric defensive mechanisms. We examined the effect of NO on cytotoxicity induced by hydrogen peroxide, and mucous glycoprotein secretion and PGE2 release from cultured rabbit gastric mucosal cells. NO enhanced cytotoxicity induced by hydrogen peroxide. Defensive prostaglandin E2 release and mucous glycoprotein secretion were not altered by NO. Under certain circumstances, NO might behave as an aggressive factor in gastric mucosal injury.
Hata
Y
Y
Department of Medicine, Veterans Affairs Medical Center, Long Beach, CA, USA.
Ota
S
S
Hiraishi
H
H
Terano
A
A
Ivey
K J
KJ
D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D004347:Drug Interactions; D005748:Gastric Fundus / Q000166:cytology / Q000187:drug effects*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects*; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D009589:Nitrogen Oxides; D009603:Nitroso Compounds / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D011817:Rabbits; D026423:S-Nitroso-N-Acetylpenicillamine; D026422:S-Nitrosoglutathione; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology
D006023:Glycoproteins; D009589:Nitrogen Oxides; D009603:Nitroso Compounds; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D026423:S-Nitroso-N-Acetylpenicillamine; D006861:Hydrogen Peroxide; D005978:Glutathione; D010396:Penicillamine; D015232:Dinoprostone