Determination of peptidoglycan-associated protein in Escherichia coli NCIB 8545 by capillary zone electrophoresis.
An efficient reliable and sensitive capillary zone electrophoresis assay for the six major bacterial peptidoglycan-associated proteins of Escherichia coli NCIB 8545 is described. The method provides the facility to determine quantitatively the effect of antibacterials on bacterial peptidoglycan-associated protein synthesis and thus to further elucidate the mechanism of antibacterial action of such drugs as the antifolates which recently have been shown to adversely affect peptidoglycan synthesis.
Xing
J Z
JZ
School of Pharmacy, Robert Gordon University, Schoolhill, Aberdeen, UK.
Richards
R M
RM
D001426:Bacterial Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010457:Peptidoglycan / Q000737:chemistry*; D012015:Reference Standards
D001426:Bacterial Proteins; D010457:Peptidoglycan