Expression of a dystrophin-sarcoglycan complex in serum-deprived BC3H1 cells and involvement of alpha-sarcoglycan in substrate attachment.
Dystrophin and alpha- and gamma-sarcoglycans were newly expressed in BC3H1 cells during differentiation induced by serum withdrawal. These proteins formed a tight complex with other dystophin-associated proteins (DAPs), as detected by immunoprecipitation with anti-dystrophin antibody. Integrins beta 1 and beta 3, vinculin, and focal adhesion kinase were also detected in the same immunoprecipitate. In a cell adhesion assay, differentiated BC3H1 cells attached more efficiently to type I collagen-coated dishes than nondifferentiated cells and loss of alpha-sarcoglycan induced by antisense ODN in differentiated cells resulted in significant inhibition of cell adhesion. Thus dystrophin and DAPs, at least partly, form a complex with the focal adhesion proteins in differentiated BC3H1 cells and alpha-sarcoglycan seems to modulate the function of the focal adhesion complex in these cells.
Yoshida
T
T
Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan.
Hanada
H
H
Iwata
Y
Y
Pan
Y
Y
Sigekawa
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001769:Blood; D015815:Cell Adhesion Molecules / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D016895:Culture Media, Serum-Free; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D016189:Dystrophin / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D015151:Immunoblotting; D016023:Integrins / Q000032:analysis; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D049031:Sarcoglycans; D016596:Vinculin / Q000032:analysis
D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D016023:Integrins; D008562:Membrane Glycoproteins; D016376:Oligonucleotides, Antisense; D049031:Sarcoglycans; D016596:Vinculin; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496051:Ptk2 protein, mouse