title: Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography.
abstract: A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.
authors.affiliation: School of Chemistry, Macquarie University, North Ryde, Australia.
authors.forename: P A
authors.initials: PA
authors.lastname: Haynes
authors.forename: D
authors.initials: D
authors.lastname: Sheumack
authors.forename: J
authors.initials: J
authors.lastname: Kibby
authors.forename: J W
authors.initials: JW
authors.lastname: Redmond
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000804:Angiotensin II / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005449:Fluorenes; D006801:Humans; D008832:Microchemistry; D009113:Muramidase / Q000737:chemistry
chemical_list: D000596:Amino Acids; D005449:Fluorenes; D000804:Angiotensin II; C054007:1-(9-fluorenyl)methyl chloroformate; D009113:Muramidase