LPS induced release of IL-1 beta, IL-6, IL-8 and TNF-alpha in EDTA or heparin anticoagulated whole blood from persons with high or low levels of serum HDL.
We have examined the lipopolysaccharide (LPS) induced release of interleukin 1 beta (IL-1 beta), IL-6, IL-8 and tumour necrosis factor alpha (TNF-alpha), in EDTA or heparin anticoagulated whole blood, from persons with high or low levels of high density lipoprotein (HDL), incubated at 37 degrees C for up to 20 h. In general, 100 ng LPS/ml gave two to twenty times higher responses than 1 ng LPS/ml, and heparin ten to hundred times higher than EDTA. The release of IL-8 was significantly higher in persons with high HDL, and was correlated with HDL, and inversely correlated with triglycerides and sCD14. The release of IL-1 beta, IL-6 and TNF-alpha was correlated with total cholesterol at medium doses of LPS (100 ng/ml), and inversely correlated with lipopolysaccharide-binding protein (LBP) at low doses of LPS (1 ng/ml). Serum levels of LBP were higher in persons with HDL, although not significantly. These results show LPS responsiveness of pro-inflammatory cytokines in whole blood from persons with high and low levels of HDL to be different, presumably of importance in inflammation and atherogenesis.
Eggesbø
J B
JB
Department of Clinical Chemistry, Ullevål University Hospital, Oslo, Norway.
Hjermann
I
I
Høstmark
A T
AT
Kierulf
P
P
D000925:Anticoagulants / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007378:Interleukins / Q000097:blood*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000925:Anticoagulants; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D014409:Tumor Necrosis Factor-alpha; D006493:Heparin; D004492:Edetic Acid