title: Determination of the C-terminal form of an anemone toxin using capillary electrophoresis and mass spectrometry.
abstract: To identify the form of the C-terminal amino acid of a sea anemone toxin, the native protein was compared with two synthetic proteins comprising the same sequence and a free or an amide C-terminal form. Using electrospray ionization-mass spectrometry, capillary electrophoresis and the coupling of both techniques, we assigned the C-terminus of the native protein to be in the free carboxyl form.
authors.affiliation: Institut de Biologie Structurale CEA-CNRS, Grenoble, France. eric.forest@ibs.fr
authors.forename: E
authors.initials: E
authors.lastname: Forest
authors.forename: Y
authors.initials: Y
authors.lastname: Pétillot
authors.forename: J
authors.initials: J
authors.lastname: Gagnon
authors.forename: J
authors.initials: J
authors.lastname: Cotton
authors.forename: C
authors.initials: C
authors.lastname: Vita
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009498:Neurotoxins / Q000138:chemical synthesis / Q000737:chemistry*; D012615:Sea Anemones / Q000378:metabolism*
chemical_list: D009498:Neurotoxins