Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography.
A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.
Haynes
P A
PA
School of Chemistry, Macquarie University, North Ryde, Australia.
Sheumack
D
D
Kibby
J
J
Redmond
J W
JW
D000596:Amino Acids / Q000032:analysis*; D000804:Angiotensin II / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005449:Fluorenes; D006801:Humans; D008832:Microchemistry; D009113:Muramidase / Q000737:chemistry
D000596:Amino Acids; D005449:Fluorenes; D000804:Angiotensin II; C054007:1-(9-fluorenyl)methyl chloroformate; D009113:Muramidase