Effect of denervation of the rat superior mesenteric arterial bed on the distribution of resistance among the first four orders of branching.
The rat superior mesenteric artery with its first four orders of branching has been examined as an isolated perfused preparation. Lumen diameter and proportional resistance at each level have been determined and compared under conditions of constant pressure following methoxamine-induced constriction and acetylcholine-induced relaxation in intact and denervated preparations. A comparison was also made between intact preparations under conditions of constant pressure and constant flow. Lumen diameter and segment length were measured by analysis of video-taped images. The distribution of resistance among the first four orders of branching was estimated from the measured values using the Poiseuille formula. Intact first and fourth order branches had a proportionately low resistance, with the remaining resistance being fairly evenly distributed between the second and third order branches. No change in the pattern occurred as a result of either methoxamine or acetylcholine treatment. The pattern of distribution of resistance changed following denervation, so that proportional resistance was high in the first order branches and fell off from the second to fourth order branches. With methoxamine, the first order branches of denervates had a significantly higher resistance than in intact tissues, with the distribution of resistance in the second to fourth order branches following the basal resistance pattern. Reduction in the proportion of resistance with acetylcholine was similar in both intact and denervated preparations. Distribution of resistance did not differ between constant flow and constant pressure intact preparations.
Scott
T M
TM
Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Chafe
L L
LL
Van Vliet
B N
BN
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D017763:Autonomic Denervation; D001794:Blood Pressure / Q000502:physiology; D008297:Male; D017538:Mesenteric Artery, Superior / Q000033:anatomy & histology / Q000294:innervation* / Q000502:physiology*; D008729:Methoxamine / Q000494:pharmacology; D018715:Microscopy, Video; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology / Q000294:innervation* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D014662:Vasoconstrictor Agents; D008729:Methoxamine; D000109:Acetylcholine