Simultaneous determination of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acids in human urine by thin layer chromatography.
A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.
Alemany
G
G
Dept. de Biologia i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Gamundí
A
A
Rosselló
C
C
Rial
R
R
D002855:Chromatography, Thin Layer; D006719:Homovanillic Acid / Q000652:urine*; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D008982:Molybdenum; D010666:Phenylpropionates / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D017973:Tungsten Compounds; D014642:Vanilmandelic Acid / Q000652:urine*
C029556:Folin's phenol reagent; D010666:Phenylpropionates; D017973:Tungsten Compounds; C001418:4-hydroxyphenyllactic acid; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; D008982:Molybdenum; D006719:Homovanillic Acid