Application of [3H]L-N(G)-nitro-arginine labelling to measure cerebellar nitric oxide synthase in patients with schizophrenia.
Brains from patients with schizophrenia have been reported to contain deficient and dysplastic forebrain neurons containing nitric oxide synthase (NOS). As part of a study of NOS in schizophrenia, we decided to investigate the cerebellum, which has particularly high levels of NOS. We used an autoradiographic method to measure the density and distribution of NOS. Sections of frozen cerebellum removed at autopsy were labelled with the selective NOS inhibitor [3H]L-NG-nitro-arginine. NOS levels were visualized in sagittal sections of vermis from 16 control subjects and 21 schizophrenia patients, and measurements were taken from the three groups of developmentally-distinct lobules I-V, VI-VII and VIII-X. The highest NOS density was in the Purkinje/molecular layer of cerebellar cortex, although there was some NOS in the granule cell layer. There were no differences in Purkinje/molecular or granule cell layer NOS levels between the two groups of subjects. The mild structural faults in cerebellar vermis observed in some patients with schizophrenia probably do not involve reductions in NOS-containing cells.
Doyle
C A
CA
School of Biological Sciences, Manchester University, UK. cdoyle@man.ac.uk
Slater
P
P
D000368:Aged; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001345:Autoradiography; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009249:NADP / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester; D009474:Neurons / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012559:Schizophrenia / Q000201:enzymology*
D004791:Enzyme Inhibitors; D009249:NADP; D001120:Arginine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester