Determination of the C-terminal form of an anemone toxin using capillary electrophoresis and mass spectrometry.
To identify the form of the C-terminal amino acid of a sea anemone toxin, the native protein was compared with two synthetic proteins comprising the same sequence and a free or an amide C-terminal form. Using electrospray ionization-mass spectrometry, capillary electrophoresis and the coupling of both techniques, we assigned the C-terminus of the native protein to be in the free carboxyl form.
Forest
E
E
Institut de Biologie Structurale CEA-CNRS, Grenoble, France. eric.forest@ibs.fr
Pétillot
Y
Y
Gagnon
J
J
Cotton
J
J
Vita
C
C
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009498:Neurotoxins / Q000138:chemical synthesis / Q000737:chemistry*; D012615:Sea Anemones / Q000378:metabolism*
D009498:Neurotoxins