title: Heat-induced alterations in embryonic cytoskeletal and stress proteins precede somite malformations in rat embryos.
abstract: Previous work from this laboratory has demonstrated that heat exposure on gestation day 10 (GD10) resulted in disrupted somite development 24 hr after exposure and subsequent thoracic skeletal malformations in neonates. The purpose of the present study was to examine the effects of in vitro heat shock on de novo protein synthesis and on cytoskeletal protein levels in developing rat embryos. Explanted GD10 embryos were exposed to temperatures of 42-42.5 degrees C for 15 min. At various times postexposure (0-27 hr). embryos were labeled with 35S-methionine and processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation. Transient enhanced de novo synthesis of 70- and 90-kD proteins was observed 1-8 hr after exposure. The 70-kD protein was identified as a eukaryotic stress protein and the presence of this protein was detected between 2 and 27 hr posttreatment. Western blot analysis was used to detect quantitative changes in total actin (microfilaments), tubulin (microtubules), and vimentin (intermediate filaments). Immediately following exposure, a reduction of total vimentin to minimal detectable levels was observed in heat-treated embryos. Levels of total vimentin remained depressed for more than 2 hr and gradually returned to control levels 4-8 hr postexposure. No change in total actin or tubulin was detected in treated embryos. The data demonstrate that heat-induced alterations in proteins comprising intermediate filaments occur concomitantly with the induction of stress proteins and precede aberrant somite morphology. These alterations in embryonic proteins may help elucidate the mechanism(s) by which skeletal malformations are produced.
authors.affiliation: Division of Life Sciences, Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20857, USA. BRF@FDADR.CDRH.FDA.GOV
authors.forename: B R
authors.initials: BR
authors.lastname: Fisher
authors.forename: D J
authors.initials: DJ
authors.lastname: Heredia
authors.forename: K M
authors.initials: KM
authors.lastname: Brown
mesh_terms: D000818:Animals; D015153:Blotting, Western; D046508:Culture Techniques; D003599:Cytoskeleton / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature / Q000009:adverse effects*; D008297:Male; D008648:Mesoderm / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014746:Vimentin / Q000032:analysis
chemical_list: D006360:Heat-Shock Proteins; D014746:Vimentin