title: Successful 24-hour rabbit heart preservation by hypothermic continuous coronary microperfusion with oxygenated University of Wisconsin Solution.
abstract: BACKGROUND
  This study assessed whether a combination of hypothermic continuous coronary microperfusion and oxygenated University of Wisconsin Solution (UWS) improves postischemic functional recovery and minimizes myocardial tissue edema.
  
  
  METHODS
  Isolated rabbit hearts were divided into four groups (n = 6 each): group I (immediate reperfusion), group II (simple cold storage in UWS), group III (hypothermic continuous coronary microperfusion with UWS), and group IV (hypothermic continuous coronary microperfusion with oxygenated UWS). Hearts in groups II, III, and IV were preserved for 24 hours. Preischemic and postischemic cardiac function was measured using a Langendorff apparatus.
  
  
  RESULTS
  Hearts in group I showed complete functional recovery, whereas cardiac output in group II was inadequate. In groups III and IV, the percentage recovery rate (post/pre) of cardiac output was 57.0% +/- 3.1% and 82.2% +/- 9.1%, respectively (p < 0.05). In groups III and IV, perfusion pressures at the end of 24-hour preservation increased from the initial 5 mm Hg to 12.3 +/- 2.7 and 8.3 +/- 1.4 mm Hg (p < 0.05), respectively. In groups I, III, and IV, the percentage tissue water content was 82.8 +/- 1.0, 86.7 +/- 1.7, and 83.8 +/- 1.6, respectively (p < 0.05 for group III versus groups I and IV). There was a significant correlation between the percentage tissue water content and coronary perfusion pressure at the end of the 24-hour preservation (r = 0.60, p = 0.040) and a significant inverse correlation between percentage tissue water content and percentage recovery rate of cardiac output (r = -0.69, p = 0.014). In ultrastructural examination, myocardial tissue edema was limited and mitochondria were well preserved in group IV.
  
  
  CONCLUSIONS
  We conclude that the combination of a hypothermic continuous coronary microperfusion technique and oxygenation of UWS was the procedure of choice for reducing tissue edema and improving both the coronary microcirculation and functional recovery during 24-hour heart preservation.
authors.affiliation: Department of Surgery, Kobe University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: C
authors.initials: C
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Okada
mesh_terms: D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D000818:Animals; D001834:Body Water / Q000378:metabolism; D002302:Cardiac Output; D002314:Cardioplegic Solutions / Q000008:administration & dosage*; D003080:Cold Temperature; D003326:Coronary Circulation; D005978:Glutathione / Q000008:administration & dosage; D006321:Heart / Q000503:physiopathology; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage; D008833:Microcirculation; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010100:Oxygen; D011817:Rabbits; D011887:Raffinose / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen