Morphological study of bacteria of the respiratory system using fluorescence microscopy of Papanicolaou-stained smears with special regard to the identification of Mycobacteria sp.
In Papanicolaou-stained smears certain structures such as nucleoli, Pneumocystis carinii, Charcot-Leyden crystals, bacteria and fungi show a brilliant fluorescence. The morphological characteristics of microorganisms which can be detected by this system, especially mycobacteria, are described. This screening method offers the possibility of providing the clinician with a provisional diagnosis within hours. Proof of the nature of the organisms should be obtained by culture.
Küpper
T
T
Institute of Cytopathology, Heinrich-Heine-University, Düsseldorf, Germany.
Steffen
U
U
Wehle
K
K
Richartz
G
G
Pfitzer
P
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D001576:Benzophenoneidum; D004396:Coloring Agents; D003363:Cost Control; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence / Q000191:economics*; D008875:Middle Aged; D009161:Mycobacterium / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis; D011237:Predictive Value of Tests; D012137:Respiratory System / Q000382:microbiology*; D012235:Rhodamines
D004396:Coloring Agents; D012235:Rhodamines; D001576:Benzophenoneidum