title: Lamellar stacking in three-dimensional crystals of Ca(2+)-ATPase from sarcoplasmic reticulum.
abstract: Electron microscopy of multilamellar crystals of CA(2+)-ATPase currently offers the best opportunity for obtaining a high-resolution structure of this ATP-driven ion pump. Under certain conditions small, wormlike crystals are formed and provide views parallel to the lamellar plane, from which parameters of lamellar stacking can be directly measured. Assuming that molecular packing is the same, data from these views could supplement those obtained by tilting large, thin platelike crystals. However, we were surprised to discover that the lamellar spacing was variable and depended on the amount of glycerol present during crystallization (20% versus 5%). Projection maps (h,0,l) from these womklike crystals suggest different molecular contacts that give rise to the different lamellar spacings. Based on an orthogonal projection map (h,k,0) from collapsed, wormlike crystals and on x-ray powder patterns, we conclude that molecular packing within the lamellar plane is the same as that in thin, platelike crystals and is unaffected by glycerol. Finally, the orientation of molecules in the lamellar plane was characterized from freeze-dried, shadowed crystals. Comparing the profile of molecules in these multilamellar crystals with that previously observed in helical tubes induced by vanadate gives structural evidence of the conformational change that accompanies binding of calcium of Ca(2+)-ATPase.
authors.affiliation: Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York 10016, USA.
authors.forename: G W
authors.initials: GW
authors.lastname: Cheong
authors.forename: H S
authors.initials: HS
authors.lastname: Young
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: C
authors.initials: C
authors.lastname: Toyoshima
authors.forename: D L
authors.initials: DL
authors.lastname: Stokes
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D005990:Glycerol; D008854:Microscopy, Electron; D008958:Models, Molecular; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D000252:Calcium-Transporting ATPases; D005990:Glycerol