title: Novel aliphatic epoxide hydrolase activities from dematiaceous fungi.
abstract: Epoxide hydrolases were found to be constitutively expressed in dematiaceous fungi coincident with secondary metabolite pigment production in stationary or idiophase. Washed-cell preparations of two fungi, Ulocladium atrum CMC 3280 and Zopfiella karachiensis CMC 3284, exhibited affinity for 2,2-dialkylated oxiranes, for which contrasting enantioselectivities were observed, but not for aromatic styrene oxide or alicyclic cyclohexene oxide type substrates. Lyophilised preparations of soluble epoxide hydrolase activities proved to be effective catalysts for the mild hydrolysis of aliphatic epoxides.
authors.affiliation: Department of Biological Sciences, Washington Singer Laboratories, University of Exeter, Devon, UK.
authors.forename: G
authors.initials: G
authors.lastname: Grogan
authors.forename: S M
authors.initials: SM
authors.lastname: Roberts
authors.forename: A J
authors.initials: AJ
authors.lastname: Willetts
mesh_terms: D001711:Biotransformation; D003510:Cyclohexanes / Q000378:metabolism; D053138:Cyclohexenes; D004851:Epoxide Hydrolases / Q000096:biosynthesis* / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D005027:Ethylene Oxide / Q000378:metabolism; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
chemical_list: D003510:Cyclohexanes; D053138:Cyclohexenes; D004852:Epoxy Compounds; C007193:cyclohexene oxide; C013690:styrene oxide; D004851:Epoxide Hydrolases; D005027:Ethylene Oxide