Pectin-supplemented enteral diet reduces the severity of methotrexate induced enterocolitis in rats.
BACKGROUND
Administration of methotrexate (MTX) to rats fed an elemental diet results in a high mortality from severe enterocolitis. Previous studies have shown that pectin is an important precursor of substrates for intestinal structure and function and may facilitate intestinal recovery after enterocolitis. The aim of this study is to evaluate the effect of pectin on MTX-induced enterocolitis in rats.


METHODS
Rats received intragastric infusion of either 1% pectin-supplemented or pectin-free elemental diet from the beginning of the study via a gastrostomy. On the 4th day animals received either MTX, 20 mg/kg intraperitoneally, or saline injection and were killed on the 7th day for sampling.


RESULTS
Pectin supplementation significantly decreased body weight loss, organ water content, and intestinal myeloperoxidase levels and increased mucosal protein, DNA, and RNA content in enterocolitis rats. The intestinal permeability was increased by administration of MTX, and pectin supplementation significantly reversed the increased permeability in the distal small bowel and colon. Pectin supplementation also lowered the magnitude of bacterial translocation, decreased plasma endotoxin levels, and restored bowel microecology.


CONCLUSIONS
Pectin significantly decreased MTX-induced intestinal injury and improved bowel integrity.
Mao
Y
Y
Dept. of Surgery, Lund University, Sweden.
Kasravi
B
B
Nobaek
S
S
Wang
L Q
LQ
Adawi
D
D
Roos
G
G
Stenram
U
U
Molin
G
G
Bengmark
S
S
Jeppsson
B
B
D000818:Animals; D018988:Bacterial Translocation; D002432:Cecum / Q000382:microbiology; D004043:Dietary Fiber / Q000008:administration & dosage / Q000627:therapeutic use*; D019446:Endotoxemia / Q000517:prevention & control; D004750:Enteral Nutrition; D004760:Enterocolitis / Q000139:chemically induced / Q000178:diet therapy*; D005526:Food, Formulated; D007082:Ileum / Q000382:microbiology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008727:Methotrexate; D010368:Pectins / Q000008:administration & dosage / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004043:Dietary Fiber; D010368:Pectins; D009195:Peroxidase; D008727:Methotrexate