Characterization of the plasmidic beta-lactamase CMY-2, which is responsible for cephamycin resistance.
The phenotype of Klebsiella pneumoniae HEL-1 indicates a plasmidic cephamycinase gene (blaCMY-2). Its sequence shows one open reading frame coding for a protein of 381 amino acids. CMY-2 is classified as class C beta-lactamase that is closely related to the plasmidic enzymes BIL-1 and LAT-1 and the chromosomal AmpC of Citrobacter freundii. The blaCMY-2 gene possibly was translocated onto a plasmid of C. freundii which spread to K. pneumoniae.
Bauernfeind
A
A
Max von Pettenkofer-Institut, Munich, Germany.
Stemplinger
I
I
Jungwirth
R
R
Giamarellou
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D018550:Cephalosporin Resistance / Q000235:genetics*; D002513:Cephamycins / Q000494:pharmacology*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000302:isolation & purification; D005798:Genes, Bacterial; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry*; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
D002513:Cephamycins; D004269:DNA, Bacterial; C103022:beta-lactamase CMY-2; D001618:beta-Lactamases