title: Experimental immunization of hamsters with an EDTA extract of Leptospira interrogans, serovar icterohaemorrhagiae.
abstract: The EDTA extract was prepared from the Leptospira interrogans serovar icterohaemorrhagiae. When inoculated subcutaneously in hamsters it conferred protection from challenge with virulent leptospires with a low dose (10 micrograms/ml) and low agglutinating antibody (40). The protection of animals obtained by the EDTA extract opens up the perspective of its use as a component of a vaccine for the control of leptospirosis.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: L T
authors.initials: LT
authors.lastname: de Queiroz-Leite
authors.forename: M
authors.initials: M
authors.lastname: Resende
authors.forename: N
authors.initials: N
authors.lastname: Ramos-Vieira M das
authors.forename: M
authors.initials: M
authors.lastname: Cota-Koury
mesh_terms: D000373:Agglutinins / Q000096:biosynthesis / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D004306:Dose-Response Relationship, Immunologic; D004492:Edetic Acid; D005069:Evaluation Studies as Topic; D007279:Injections, Subcutaneous; D007668:Kidney / Q000382:microbiology; D007921:Leptospira interrogans / Q000737:chemistry / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D008647:Mesocricetus; D012997:Solvents; D014611:Vaccination
chemical_list: D000373:Agglutinins; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D012997:Solvents; D004492:Edetic Acid