Validation of the SOS/umu test using test results of 486 chemicals and comparison with the Ames test and carcinogenicity data.
The present study gives a comprehensive update of all umu genotoxicity assay results published so far. The available data of 486 chemicals investigated with the umu test are compared with the Ames test (274 compounds) as well as rodent carcinogenicity data (179 compounds). On the whole, there is good agreement between the umu test and the Ames test results, with a concordance of about 90%. The umu test was able to detect 86% of the Ames mutagens, while the Ames test (using at least 5 strains) detected 97% of the umu positive compounds. The elimination of TA102 from the set of Ames tester strains reduced the percentage of detectable umu genotoxins from 97 to 86%. The agreement between carcinogenesis and umu response was 65%, which is comparable to earlier studies concerning rodent carcinogenesis and Salmonella mutagenesis. The present compilation of umu results provides a database that can be used for the comparison of the SOS-inducing activity of chemicals and their mutagenicity, respectively, carcinogenicity. The results presented here clearly demonstrate that a chemical which induces the expression of the umu operon can be regarded a rodent carcinogen with a high degree of certainty (93%).
Reifferscheid
G
G
AMMUG, University of Mainz, Germany. reiffers@goofy.adv.uni-mainz.de
Heil
J
J
D000818:Animals; D001426:Bacterial Proteins; D015197:Carcinogenicity Tests / Q000592:standards; D004259:DNA-Directed DNA Polymerase; D016208:Databases, Factual; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000592:standards; D009876:Operon; D015203:Reproducibility of Results; D012377:Rodentia; D013014:SOS Response, Genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli