Random amplified polymorphic DNA (RAPD) analysis of Escherichia coli strains: comparison of urinary and concomitant blood isolates of urosepsis patients.
The discriminatory power of random amplified polymorphic DNA (RAPD) analysis was assessed for detection of intraspecies variation in Escherichia coli strains of clinical origin. Three primers (OPF 5, OPF 7 and OPF 8) were preselected from commercial 10-mer primers by the number of distinct bands obtained. These primers were used in testing 26 urinary and 13 blood isolates from 26 patients and E. coli ATCC 25922, OPF 5, OPF 7 or OPF 8 alone separated the strains into 15 to 21 RAPD types. A combination of the results of the three primers gave 25 RAPD types. When blood and urine isolates of each patient were analysed in parallel, all blood-urine pairs were found identical, and with one exception they were also unique. RAPD analysis had a high discriminatory power. It separated the strains equally well or better than ribotyping, and obviously better than serotyping which grouped the urine strains into 8 serogroups leaving 18 strains untypable or incompletely typed. Thus, to verify the identity or non-identity of isolated E. coli strains, RAPD analysis was shown to be a sensitive and reproducible technique which is technically less demanding, more rapid and more economical than either serotyping or ribotyping. However, in its present application, this technique cannot fully replace determination of the serotype or virulence factors which may show correlations with different manifestations of infection.
Kärkkäinen
U M
UM
Department of Clinical Microbiology, Kuopio University Hospital, Finland.
Kauppinen
J
J
Ikäheimo
R
R
Katila
M L
ML
D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000097:blood* / Q000382:microbiology* / Q000652:urine; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D014552:Urinary Tract Infections / Q000097:blood* / Q000382:microbiology* / Q000652:urine