Activation of the lamin A gene during rat liver development.
We have studied the regulation of expression of the A-type lamins, which are constituents of the nuclear lamina. During rat liver development, high levels of lamin A and C mRNAs were observed in 15-day fetal liver but were barely detectable in the adult. The chromatin conformation of the lamin A gene was sensitive to DNase I in 15-day fetal liver but became mostly insensitive in the adult. Lamin A and C proteins could be detected in fetal liver and persisted in the adult. Our evidence suggests that the lamin A gene is actively transcribed early in liver differentiation and its activity declines considerably in adult liver.
Hamid
Q A
QA
Centre for Cellular and Molecular Biology, Hyderabad, India.
Fatima
S
S
Thanumalayan
S
S
Parnaik
V K
VK
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D034904:Lamin Type A; D034882:Lamins; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D002843:Chromatin; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D003850:Deoxyribonuclease I