title: Vitamin D receptor and growth inhibition by 1,25-dihydroxyvitamin D3 in human malignant melanoma cell lines.
abstract: The expression of vitamin D receptors (VDR) and growth inhibition induced by 1,25-dihydroxyvitamin D3 have been noted in certain human malignant melanoma cell lines. In this study, widely disparate levels of VDR mRNA expression were demonstrated in a panel of eight human malignant melanoma cell lines. Quantitation of receptor level by ligand binding assay showed a similar pattern. Proliferation and growth curve analysis was performed in two cell lines: RPMI 7951 (high VDR) and SK-MEL-28 (low VDR). Significant growth inhibition was noted in RPMI 7951 cells at 10(-9) M 1,25-dihydroxyvitamin D3. SK-MEL-28 cells, which express much lower levels of VDR, did not show any growth inhibition except at extremely high concentrations of 1,25-dihydroxyvitamin D3, namely 10(-5) M. These findings suggest a receptor-mediated mechanism of growth inhibition for 1,25-dihydroxyvitamin D3 and a role for this hormone in the growth of malignant melanoma cells.
authors.affiliation: Department of Surgery, Lombardi Cancer Center, Georgetown University Hospital, Washington, DC 20007, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Evans
authors.forename: A M
authors.initials: AM
authors.lastname: Houghton
authors.forename: L
authors.initials: L
authors.lastname: Schumaker
authors.forename: R V
authors.initials: RV
authors.lastname: Brenner
authors.forename: R R
authors.initials: RR
authors.lastname: Buras
authors.forename: F
authors.initials: F
authors.lastname: Davoodi
authors.forename: R J
authors.initials: RJ
authors.lastname: Nauta
authors.forename: M
authors.initials: M
authors.lastname: Shabahang
mesh_terms: D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D018167:Receptors, Calcitriol / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D018167:Receptors, Calcitriol; D002117:Calcitriol