title: Effect of hypotension preceding death on the function of lungs from donors with nonbeating hearts.
abstract: BACKGROUND
  A shortage of suitable brain-dead donors continues to severely limit lung transplantation. Use of donors with nonbeating hearts has been suggested as a solution. Lungs are unique, in that aerobic metabolism can continue in the absence of blood circulation because oxygen is present in airways and alveoli. Animal studies have shown reasonable cadaveric graft function up to several hours after sudden death by drug administration. However, hemodynamic instability before death may worsen lung function through activation and pulmonary sequestration of neutrophils and release of inflammatory mediators. Because many potential cadaveric donors experience hypotension before death, this study was undertaken to assess the effect of hypotensive shock on cadaveric lung viability.
  
  
  METHODS
  A rat isolated lung reperfusion model was used to assess pulmonary function over 3 hours of reperfusion or until gross pulmonary edema developed. Twenty-five rats were randomly allocated to the following study groups, which were based on status before lung harvest: (1) control: no interventions; (2) hypotensive: 1 hour of hypotension by exsanguination to a mean blood pressure of 30 to 40 mm Hg; (3) cadaver: death by cervical dislocation followed by 3 hours of in situ lung ischemia; (4) hypotensive + 3 hours cadaver: 1 hour of hemorrhagic shock, followed by death and 3 hours of in situ ischemia; (5) hypotensive + 2 hours cadaver: similar to group 4, except the in situ ischemia was abbreviated to 2 hours.
  
  
  RESULTS
  No significant differences were found among group 1, 2, or 3 lungs with regard to wet to dry weight ratios, gas exchange, and pulmonary arterial or airway pressures. However, all group 4 lungs became grossly hemorrhagic and developed severe pulmonary edema within 10 minutes of reperfusion. Group 5 lungs fared only marginally better, with two of five lungs tolerating 3 hours of reperfusion.
  
  
  CONCLUSIONS
  A period of hypotension before death severely impairs cadaveric lung viability.
authors.affiliation: Department of Surgery, Toronto Hospital, Ontario, Canada.
authors.forename: L N
authors.initials: LN
authors.lastname: Tremblay
authors.forename: T
authors.initials: T
authors.lastname: Yamashiro
authors.forename: K N
authors.initials: KN
authors.lastname: DeCampos
authors.forename: B V
authors.initials: BV
authors.lastname: Mestrinho
authors.forename: A S
authors.initials: AS
authors.lastname: Slutsky
authors.forename: T R
authors.initials: TR
authors.lastname: Todd
authors.forename: S H
authors.initials: SH
authors.lastname: Keshavjee
mesh_terms: D000818:Animals; D001926:Brain Death / Q000503:physiopathology*; D006801:Humans; D007022:Hypotension / Q000503:physiopathology*; D016040:Lung Transplantation / Q000502:physiology*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D011654:Pulmonary Edema / Q000503:physiopathology; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D011669:Pulmonary Wedge Pressure / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012769:Shock / Q000503:physiopathology; D014019:Tissue Donors; D014022:Tissue Survival / Q000502:physiology*; D009927:Tissue and Organ Procurement
chemical_list: D009195:Peroxidase