Effect of camel urine on the cytological and biochemical changes induced by cyclophosphamide in mice.
Camel urine treatment was found to cause a significant cytotoxic effect in the bone marrow cells of mice. This cytotoxicity at higher doses was comparable with that of standard drug cyclophosphamide (CP). However, unlike CP, the camel urine treatment failed to induce any clastogenicity. The cytotoxicity induced by camel urine treatment was substantiated by the reduction of liver nucleic acids and glutathione levels and increased malondialdehyde (MDA) contents in the same animals. CP treatment was found to be highly clastogenic, cytotoxic and it reduced the levels of nucleic acids, proteins, glutathione and increased malondialdehyde concentration due to its prooxidant nature. The non-clastogenic nature of camel urine was attributed to the antioxidant and antimutagenic compounds present in camel urine. Pretreatment with camel urine increased the cytotoxicity of CP and intensified the CP induced reduction of liver nucleic acids, glutathione and increased the MDA concentration. The increase of CP induced cytotoxicity appears to be partly due to the additive effect of the two treatments on cellular lipid peroxidation.
al-Harbi
M M
MM
Department of Pharmacology, College of Pharmacy, King Saud University, Saudi Arabia.
Qureshi
S
S
Ahmed
M M
MM
Raza
M
M
Baig
M Z
MZ
Shah
A H
AH
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D002162:Camelus; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005269:Femur / Q000166:cytology; D005978:Glutathione / Q000378:metabolism; D049673:History, 20th Century; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008514:Medicine, Arabic; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D009153:Mutagens / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D014556:Urine
D000970:Antineoplastic Agents; D018906:Antineoplastic Agents, Alkylating; D009153:Mutagens; D008315:Malondialdehyde; D012313:RNA; D003520:Cyclophosphamide; D004247:DNA; D005978:Glutathione