In vitro NMR proton T1 measurements in peritoneal ascites.
The proton spin-lattice relaxation rate (1/T1) in malignant and non-malignant ascites was measured with an FT NMR spectrometer operating at 60 MHz. The mean relaxation rate in non-malignant ascites was significantly smaller than that of malignant ascites. However, the 1/T1 of malignant ascites overlaps with that of non-malignant ascites over all concentrations of total protein (TP) in samples. The 1/T1 in non-malignant ascites correlates strongly with TP, whilst the 1/T1 in malignant ascites shows only a moderate correlation. T1 measurements before and after addition of ascorbic acid (reductant) suggest that there is a small paramagnetic contribution of ions to the 1/T1 in malignant ascites. The least-squares fitting of 1/T1 versus TP for non-malignant data gives a linear relationship, and suggests that the T1 mechanism in non-malignant ascites is caused by a fast chemical exchange of water molecules between protein-bound water and free water.
Yilmaz
A
A
Department of Physics and Mathematics, Faculty of Science, Dicle University, Diyarbakir, Turkey.
Tez
M
M
Göral
V
V
Boylu
S
S
Kaplan
A
A
Kavak
G
G
D001201:Ascites / Q000378:metabolism*; D001205:Ascorbic Acid / Q000737:chemistry; D055592:Biophysical Phenomena; D001703:Biophysics; D003300:Copper / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis; D009369:Neoplasms / Q000737:chemistry; D010084:Oxidation-Reduction; D011506:Proteins / Q000032:analysis; D011522:Protons
D009363:Neoplasm Proteins; D011506:Proteins; D011522:Protons; D003300:Copper; D007501:Iron; D001205:Ascorbic Acid