title: Cloning and characterization of murine endothelial constitutive nitric oxide synthase.
abstract: Complementary DNA clones encoding mouse endothelial constitutive nitric oxide synthase (ecNOS) were isolated by plaque hybridization from a murine fetal cardiac lambda ZAP II cDNA expression library using a full-length human ecNOS cDNA as the hybridization probe. DNA sequence analysis indicates a 1202 amino acid protein showing significant sequence identity with human as well as bovine ecNOS.
authors.affiliation: Department of Medicine, St. Michael's Hospital, University of Toronto, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Gnanapandithen
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: C L
authors.initials: CL
authors.lastname: Kau
authors.forename: R M
authors.initials: RM
authors.lastname: Gorczynski
authors.forename: P A
authors.initials: PA
authors.lastname: Marsden
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D019001:Nitric Oxide Synthase