Spontaneous and lipopolysaccharide-stimulated secretion of cytokines by peripheral blood monocytes in IgA nephropathy is inhibited by interleukin-10.
The effect of recombinant human interleukin-10 (IL-10) on the in vitro cytokine secretion of peripheral blood monocytes (PBM) was evaluated in patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha and interleukin-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study IL-10 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. A significant inhibitory effect of IL-10 was observed in cultures of PBM from IgAN patients as well as from normal controls. When both LPS and anti-IL-10 antibody were added together to the PBM, a further increase in LPS-enhanced secretion of cytokines occurred. This study provides some novel information showing the inhibitory effects of IL-10 on cytokine secretion by PBM derived from IgAN patients.
Matsumoto
K
K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005922:Glomerulonephritis, IGA / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10