title: Nucleotide sequence of the gene encoding pepstatin-insensitive acid protease B, scytalidopepsin B, of Scytalidium lignicolum.
abstract: A chromosomal DNA fragment of Scytalidium lignicolum that encodes the mature enzyme region of acid protease B (Scytalidopepsin B), was cloned and its nucleotides sequenced. The fragment contained a 76-bp intron at the middle of the mature enzyme-coding region. The mature enzyme was composed of 206 amino acid residues with a molecular weight of 21,550. There were some discrepancies between the amino acid sequence deduced from these results and that previously established by protein sequencing.
authors.affiliation: School of Pharmaceutical Sciences, Nagasaki University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kakimori
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: H
authors.initials: H
authors.lastname: Oyama
authors.forename: N
authors.initials: N
authors.lastname: Oda
authors.forename: Y
authors.initials: Y
authors.lastname: Gotoh
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: S
authors.initials: S
authors.lastname: Murao
authors.forename: D
authors.initials: D
authors.lastname: Tsuru
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D003904:Mitosporic Fungi / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010436:Pepstatins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000494:pharmacology*; D015183:Restriction Mapping
chemical_list: D004271:DNA, Fungal; D010436:Pepstatins; D011480:Protease Inhibitors; D016282:Aspartic Acid Endopeptidases; C041236:Scytalidium lignicolum acid proteases