title: A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes.
abstract: Oligonucleotide primers were designed and successfully applied to amplify DNA fragments of dNDP-glucose dehydratase genes from actinomycete species producing natural compounds which contain deoxysugar moieties. The deduced amino acid sequence of the isolated fragments revealed similarity to known dNDP-glucose dehydratases. A phylogeny for the deduced proteins of the obtained fragments and for dNDP-glucose dehydratases described in the data bases was constructed. dNDP-glucose dehydratases from actinomycetes were more related to each other than to dehydratases from species of other orders. The phylogenetic analysis also revealed a close relation between dehydratases from strains producing natural compounds with similar deoxysugar moieties.
authors.affiliation: Eberhard-Karls-Universität Tübingen, Mikrobiologie, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Decker
authors.forename: S
authors.initials: S
authors.lastname: Gaisser
authors.forename: S
authors.initials: S
authors.lastname: Pelzer
authors.forename: P
authors.initials: P
authors.lastname: Schneider
authors.forename: L
authors.initials: L
authors.lastname: Westrich
authors.forename: W
authors.initials: W
authors.lastname: Wohlleben
authors.forename: A
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D000190:Actinomyces / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005820:Genetic Testing; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics
chemical_list: D006836:Hydro-Lyases