title: Microregional blood flow in murine and human tumours assessed using laser Doppler microprobes.
abstract: A multichannel laser Doppler system has been used to measure microregional fluctuations in perfusion in the HT29 human tumour xenograft and in patients with advanced malignant disease. A comparison is made with previously obtained data for the SaF, a transplantable murine tumour. The 300 microns diameter probes recorded fluctuations in erythrocyte flux in tumour microregions with an estimated volume of 10(-2) mm3. Of the 66 human tumour microregions sampled, 26% showed a change in erythrocyte flux by a factor of 2 or more over the 60 min measurement period, compared with 37% of HT29 and 48% of SaF microregions. In each of the studies more than 50% of changes were completed within 20 min, although slower changes were more common in the human tumours than in the experimental systems. Within the 1 h monitoring period at least 30% of the changes were reversed (human tumours 30%, HT29 45%, SaF 31%). These findings demonstrate that microregional changes in erythrocyte flux, consistent with transient, perfusion-driven changes in oxygenation, are a feature of human malignancies as well as experimental transplanted tumours.
authors.affiliation: Tumour Microcirculation Group, Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Hill
authors.forename: K H
authors.initials: KH
authors.lastname: Pigott
authors.forename: M I
authors.initials: MI
authors.lastname: Saunders
authors.forename: M E
authors.initials: ME
authors.lastname: Powell
authors.forename: S
authors.initials: S
authors.lastname: Arnold
authors.forename: A
authors.initials: A
authors.lastname: Obeid
authors.forename: G
authors.initials: G
authors.lastname: Ward
authors.forename: M
authors.initials: M
authors.lastname: Leahy
authors.forename: P J
authors.initials: PJ
authors.lastname: Hoskin
authors.forename: D J
authors.initials: DJ
authors.lastname: Chaplin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003110:Colonic Neoplasms / Q000098:blood supply*; D006801:Humans; D017078:Laser-Doppler Flowmetry; D051379:Mice; D008808:Mice, Inbred CBA; D016513:Mice, SCID; D008875:Middle Aged; D012039:Regional Blood Flow; D012513:Sarcoma, Experimental / Q000098:blood supply*