title: Heating or freezing bone. Effects on angiogenesis induction and growth potential in mice.
abstract: We have characterized the effect of bone graft treatment by heating or freezing (with or without dimethyl sulfoxide (DMSO)). Tissue culture and dorsal skin-fold chambers in mice were used as sites to quantify the effect on angiogenesis, growth and calcification of neonatal femora. Fresh femora increased in both length and cartilage diameter (calcification in vivo only), but cryopreservation or heating abolished the increase in femoral dimensions. In vivo, femora of all experimental groups elicited an angiogenic response from the host tissue, which was most pronounced for fresh femora, weaker for DMSO-preserved frozen bone and poor for unprotected frozen bone and boiled femora. Freezing in the presence of a cryopreservative (DMSO) was found to preserve the angiogenic potential of frozen bone, whereas unprotected heating or freezing significantly impaired angiogenesis induction and growth potential.
authors.affiliation: Department of Radiation Oncology, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Leunig
authors.forename: F
authors.initials: F
authors.lastname: Yuan
authors.forename: D A
authors.initials: DA
authors.lastname: Berk
authors.forename: L E
authors.initials: LE
authors.lastname: Gerweck
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016025:Bone Transplantation / Q000009:adverse effects; D046508:Culture Techniques; D005269:Femur / Q000254:growth & development / Q000502:physiology; D005615:Freezing; D006361:Heating; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012884:Skin Window Technique