Widespread expression of human cysteine string proteins.
We present the nucleotide and deduced amino acid sequence of a human systeine string protein (csp) and a unique truncated csp variant that derives from the retention of an exonic sequence that introduces a premature, in-frame stop codon. Low stringency Southern analysis is compatible with the presence of a single human csp gene. Northern analysis reveals that human csp mRNA has both a more heterogeneous size distribution and a more widespread tissue distribution than previously reported for other csps. Exemplifying this is the fact that csp immunoreactivity is detected in Triton X-114 extracts of human blood, an observation which may facilitate blood-based diagnostic assays of csp status in man.
Coppola
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Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095, USA.
Gundersen
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003545:Cysteine / Q000737:chemistry*; D018076:DNA, Complementary / Q000032:analysis; D050956:HSP40 Heat-Shock Proteins; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping
D018076:DNA, Complementary; D050956:HSP40 Heat-Shock Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C088460:cysteine string protein; D003545:Cysteine