A non-expressed transgene as a cell marker for the investigation of cellular traffic after splenic autotransplantation.
The investigation of cellular interactions during the regeneration of splenic transplants depends upon distinguishing host cells from graft derived cells. As a cell marker which, in contrast to many other marker systems, does not affect histocompatibility, the non-expressed transgene mMT-HGHRH-1 was introduced by mating into NMRI inbred mice to generate the transgenic mouse line BSM. By transplanting non-transgenic NMRI splenic tissue into transgenic BSM hosts, host derived cells could be identified in the transplants by in situ hybridization after regeneration. In a reciprocal approach, implant derived cells were detected in a transgenic transplant after 3 weeks of regeneration in a non-transgenic host. Relative amounts of transgenic cells in transplant cross-sections were estimated from the signal intensity of autoradiographs by densitometry and computer analysis. This approach could be broadly applied in studies of transplantation systems.
Hammerl
P
P
University of Salzburg, Department of Biochemistry, Austria.
Weinlich
D
D
Hartl
A
A
Thalhamer
J
J
D000818:Animals; D002470:Cell Survival; D002843:Chromatin / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D012038:Regeneration / Q000502:physiology*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D019076:Transgenes
D002843:Chromatin; D005819:Genetic Markers