title: PCR for identification and typing of Helicobacter pylori isolated from children.
abstract: From 191 children with upper gastroduodenal disease 236 gastric biopsy specimens were taken. H. pylori was detected by use of culture Gram staining histological examination and PCR technique. A segment of DNA coding protein synthesis of 26 kDa or urease A and B gene were used for PCR amplification. PCR technique was also used for determination of the presence of Cag A gene in 72 strains of H. pylori isolated from children. Genetic typing of H. pylori strains by RFLP analysis of PCR amplified urease B gene 933 bp fragment and RAPD were performed. Biopsy specimens taken from children with gastritis were in 52% H. pylori culture and PCR positive, while 18.1% PCR positive only. Similarly, specimens taken from children with duodenal ulcer were in 50% H. pylori culture and PCR positive, while 12.5% PCR positive only. Fifty one (70.8%) from 72 strains of H. pylori were Cag A positive. Molecular typing of the strains isolated during first and follow-up endoscopy allowed the differentiation between reinfection or new infection and coinfection. It was shown that RAPD typing had better discrimination power in comparison to PCR--RFLP method.
authors.affiliation: Department of Clinical Microbiology, Children's Memorial Health Institute, Warsaw, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Dzierzanowska
authors.forename: A
authors.initials: A
authors.lastname: Gzyl
authors.forename: E
authors.initials: E
authors.lastname: Rozynek
authors.forename: E
authors.initials: E
authors.lastname: Augustynowicz
authors.forename: U
authors.initials: U
authors.lastname: Wojda
authors.forename: D
authors.initials: D
authors.lastname: Celińska-Cedro
authors.forename: M
authors.initials: M
authors.lastname: Sankowska
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D002648:Child; D004381:Duodenal Ulcer / Q000382:microbiology*; D004386:Duodenum / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology*; D014510:Urease / Q000235:genetics*
chemical_list: D007074:Immunoglobulin G; D014510:Urease