title: Intron position affects expression from the tpi promoter in rice.
abstract: A series of promoter-GUS fusion constructs containing a portion of the rice triosephosphate isomerase (tpi) promoter, the first tpi intron, and the gene encoding bacterial beta-glucuronidase (GUS) were made. These constructs were electroporated into rice protoplasts and transient expression was monitored. Inclusion of the first intron from the rice tpi gene enhanced expression of the GUS gene from the tpi promoter when it was placed 5' of the GUS gene. When the tpi intron was placed in the 3'-untranslated region no enhancement of GUS gene expression was observed, indicating the importance of position in intron-mediated enhancement of gene expression.
authors.affiliation: Institute of Developmental and Molecular Biology, Texas A&M University, College Station 77843-3155, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Snowden
authors.forename: W G
authors.initials: WG
authors.lastname: Buchhholz
authors.forename: T C
authors.initials: TC
authors.lastname: Hall
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018274:Electroporation; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000096:biosynthesis; D007438:Introns; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011523:Protoplasts; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection; D014305:Triose-Phosphate Isomerase / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D005966:Glucuronidase; D014305:Triose-Phosphate Isomerase