A ciliary neurotrophic factor-sensitive human myeloma cell line.
We obtained a human myeloma cell line (XG4-CNTF) whose growth was completely dependent on addition of ciliary neurotrophic factor (CNTF). Half-maximal proliferation was induced by adding 20 pg/mL CNTF. Response to CNTF correlated with expression of membrane CNTF receptor alpha-chain (CNTFR alpha), as shown by PCR analysis and immunostaining with anti-CNTFR alpha antibodies. CNTF-induced proliferation was completely inhibited by antibodies to gp130 interleukin-6 (IL-6) transducer, unlike antibodies to IL-6 or IL-6 receptor (IL-6R). Growth of XG4-CNTF cells using the gp130 IL-6 transducer was also supported by other cytokines: IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OM). This cell line should be very useful for studying the interactions of IL-6-related cytokines with their receptors.
Gu
Z J
ZJ
Institute for Molecular Genetics, Montpellier, France.
Zhang
X G
XG
Hallet
M M
MM
Lu
Z Y
ZY
Wijdenes
J
J
Rossi
J F
JF
Klein
B
B
D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology / Q000494:pharmacology / Q000502:physiology; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000494:pharmacology; D009101:Multiple Myeloma; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D020801:Receptor, Ciliary Neurotrophic Factor; D018121:Receptors, Cytokine / Q000096:biosynthesis*; D018123:Receptors, Interleukin / Q000276:immunology / Q000502:physiology; D019947:Receptors, Interleukin-6; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis*; D053606:Receptors, OSM-LIF; D014407:Tumor Cells, Cultured
D000906:Antibodies; D015703:Antigens, CD; D020934:Ciliary Neurotrophic Factor; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020801:Receptor, Ciliary Neurotrophic Factor; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D017475:Receptors, Nerve Growth Factor; D053606:Receptors, OSM-LIF