Capillary electrophoresis of heparin and dermatan sulfate unsaturated disaccharides with triethylamine and acetonitrile as electrolyte additives.
Capillary electrophoresis at constant voltage with the addition of triethylamine as electrolyte to a running buffer containing borate using fused-silica capillaries permits the complete resolution in less than 30 min of 11 standard heparin and 8 standard dermatan sulfate disaccharides, which represent degradation products of heparin and dermatan sulfate by specific lyases. Triethylamine influences the migration time of disaccharides by reducing both their electrophoretic mobility towards the anode and the electroosmotic flow towards the cathode. A modulated combination of these effects together with borate-disaccharide complex formation is responsible for separation, especially in the case of isomers which differ in the position of the sulfate groups. The addition of acetonitrile did not introduce any favourable effect in the separation of disaccharide mixtures. Under these conditions different dermatan sulfates were analysed to assess the source of the preparations.
Scapol
L
L
Alfa Wassermann S.p.A., Bologna, Italy.
Marchi
E
E
Viscomi
G C
GC
D000097:Acetonitriles; D000818:Animals; D001888:Boric Acids; D002021:Buffers; D002240:Carbohydrate Sequence; D003871:Dermatan Sulfate / Q000302:isolation & purification*; D004187:Disaccharides / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005021:Ethylamines; D006493:Heparin / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012867:Skin / Q000737:chemistry; D013552:Swine
D000097:Acetonitriles; D001888:Boric Acids; D002021:Buffers; D004187:Disaccharides; D005021:Ethylamines; D046911:Macromolecular Substances; D003871:Dermatan Sulfate; D006493:Heparin; C032688:boric acid; C016162:triethylamine; C032159:acetonitrile