Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface.
The lung is comprised of about 40 different cell types, of which only 15% are type II cells. These are the major, if not the sole, source of synthesis and secretion of lung surfactant. To date a large number of methods have been described for the isolation of pure populations of type II cells using a wide variety of techniques, but most of these have employed differential centrifugation methods and have used adult rodents. The present study reports the isolation of type II cells from fetal rabbit lungs by the immunoglobin G plating method. Pure populations of fetal type II cells in high yield and with good viability were obtained by the procedure for the first time from rabbit fetal tissue.
Gupta
A
A
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kaushal
S
S
Majumdar
S
S
Sanyal
S N
SN
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004847:Epithelial Cells; D005333:Fetus; D007074:Immunoglobulin G; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000196:embryology* / Q000648:ultrastructure; D011817:Rabbits
D007074:Immunoglobulin G