Simple method for detecting fluconazole-resistant yeasts with chromogenic agar.
A method for detecting fluconazole-resistant yeasts was developed that uses chromogenic agar containing fluconazole. Yeasts were plated on media with fluconazole at 0, 8, and 16 micrograms/ml. On media without fluconazole, normal growth of susceptible yeasts (defined as those having a fluconazole MIC of < 8 micrograms/ml) was detected, while fluconazole-containing media suppressed susceptible strains and normal colonies of resistant yeasts (fluconazole MICs of > or = 8 micrograms/ml) were detected. This method was used to screen for resistance in oropharyngeal candidiasis. Isolates having fluconazole MICs of > or = 8 micrograms/ml and < 8 micrograms/ml were correctly predicted in 43 of 45 cultures and 115 of 116 cultures, respectively. This screening method appears to be rapid and sensitive for detection of fluconazole-resistant yeasts.
Patterson
T F
TF
Department of Medicine, University of Texas Health Science Center at San Antonio 78284, USA. PATTERSON@UTHSCSA.EDU
Revankar
S G
SG
Kirkpatrick
W R
WR
Dib
O
O
Fothergill
A W
AW
Redding
S W
SW
Sutton
D A
DA
Rinaldi
M G
MG
D000362:Agar; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology; D002863:Chromogenic Compounds; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D009172:Mycology / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*
D000935:Antifungal Agents; D002863:Chromogenic Compounds; D003470:Culture Media; D015725:Fluconazole; D000362:Agar