Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments.
Four plasmids, each encoding a combination of either an Escherichia coli or Pseudomonas putida promoter and either toluene dioxygenase or toluene monooxygenase, were electroporated into five bacterial strains isolated from sediments found at depths of 91 to 295 m. Four of these engineered bacterial strains demonstrated both toluene and trichloroethylene degradation activities.
Romine
M F
MF
Pacific Northwest National Laboratory, Richland, Washington 99352, USA. mf_romine@pnl.gov
Brockman
F J
FJ
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics; D012988:Soil Microbiology; D014050:Toluene / Q000378:metabolism*; D014241:Trichloroethylene / Q000378:metabolism*
D017931:DNA Primers; D004269:DNA, Bacterial; D014241:Trichloroethylene; D014050:Toluene; D010105:Oxygenases; C023172:toluene dioxygenase; C068610:toluene-4-monooxygenase