Cloning of a novel signal-transducing adaptor molecule containing an SH3 domain and ITAM.
We molecularly cloned a cDNA coding for a novel phosphotyrosine molecule with a 70 kDa molecular mass, named STAM (signal transducing adaptor molecule), which is tyrosine-phosphorylated rapidly after stimulation with various cytokines such as IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF and PDGF. STAM contains an SH3 (Src-homology 3) domain and the ITAM (immunoreceptor tyrosine-based activation motif), suggesting that STAM acts as an adaptor molecule involved in signal transducing pathways from the cytokine receptors.
Takeshita
T
T
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Arita
T
T
Asao
H
H
Tanaka
N
N
Higuchi
M
M
Kuroda
H
H
Kaneko
K
K
Munakata
H
H
Endo
Y
Y
Fujita
T
T
Sugamura
K
K
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D016207:Cytokines; D018076:DNA, Complementary; D056827:Endosomal Sorting Complexes Required for Transport; D010750:Phosphoproteins; D011506:Proteins; D018121:Receptors, Cytokine; C109775:STAM protein, human; C491648:Stam protein, mouse; D014443:Tyrosine