[Kinetics of sialylation of Neisseria gonorrhoeae by radiolabelled cytidine 5'monophospho-N-acetyl neuraminic acid].
Neisseria gonorrhoeae (Strain A) induces a sialyl-transferase when treated with a very low concentration of cytidine 5'monophospho-N-acetyl neuraminic acid, 2 x 10(-3) nmol.ml-1, a concentration which is insufficient to produce an adequate resistance to human serum complement. The sialyl-transferase activity was detected by measurement of fixed 14C radio-labeled sialyl groups. Without this stimulation, there was practically no transfer of sialyl groups. The gonococcal sialyl-transferase could be considered inducible.
Goldner
M
M
Département de Microbiologie, Université Laval, SAINTE-FOY, Québec, Canada.
Emond
J P
JP
Germani
E
E
Launay
J M
JM
Dublanchet
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002701:Chloramphenicol / Q000494:pharmacology; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000378:metabolism; D011874:Radiometry; D012799:Sialyltransferases / Q000493:pharmacokinetics*; D013268:Stimulation, Chemical
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D002701:Chloramphenicol; D012799:Sialyltransferases
Cinétique de sialylation de Neisseria gonorrhoeae par l'acide cytidine 5'monophospho-n-acétyl neuraminique radio-marqué.