Structure of the phenylalanine hydroxylase gene in Drosophila melanogaster and evidence of alternative promoter usage.
The complete Drosophila melanogaster phenylalanine hydroxylase gene isolated from a genomic library was sequenced. Gene structure consisted of five exons covering a region of around 3 kb. Position of introns in the C-terminal domain was conserved with mammalian aromatic amino acid hydroxylase genes. Putative promoter sequences in the 5'UTR and intron 1 were identified. A novel transcript was detected differing from that previously reported by the inclusion of a part of the intron 1 sequence. It could be produced using an alternative promoter. The deduced open reading frame would code a protein with a small difference at the N-terminus. Expression of the alternative transcripts was examined throughout development.
Ruiz-Vázquez
P
P
Department de Genètica, Universitat de València, Burjassot, Spain.
Moulard
M
M
Silva
F J
FJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D017344:Genes, Insect; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D010651:Phenylalanine Hydroxylase / Q000096:biosynthesis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D010651:Phenylalanine Hydroxylase