ELISPOT for detecting antibody-secreting cells in response to infections and vaccination.
Based on immunoassay principles, methods have been developed for the analysis of secreted products at the cellular level. This approach offers substantial methodological advantages compared to traditional immunoassays. In a number of applications cell-based methods have proved able to overcome many of the problems inherent to immunoassays of biological fluids. This review focuses on applications of ELISPOT in natural infections and vaccinations of human individuals. The studies reviewed here have contributed to our understanding of the B-cell responses in infections and the independence of mucosal and systemic immune responses. Whilst diagnostic applications are rare, enzyme immunospot assays have been extensively used in testing the immunogenicity of vaccines. In particular, B-cell responses to mucosal vaccines are better covered with this cellular assay.
Arvilommi
H
H
National Public Health Institute, Turku, Finland.
D000921:Antibody-Producing Cells / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007239:Infections / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
D014612:Vaccines