title: Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433.
abstract: The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [Table: see text]
authors.affiliation: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
authors.forename: G W
authors.initials: GW
authors.lastname: Robijn
authors.forename: R
authors.initials: R
authors.lastname: Gutiérrez Gallego
authors.forename: D J
authors.initials: DJ
authors.lastname: van den Berg
authors.forename: H
authors.initials: H
authors.lastname: Haas
authors.forename: J P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D014574:Uronic Acids
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids