The limb/LPM enhancer of the murine Hoxb6 gene: reporter gene analysis in transgenic embryos and studies of DNA-protein interactions.
Homeobox genes are expressed in stage-, region-, and tissue-specific patterns during embryonic development of the mouse. In order to study cis-acting regulatory elements of murine homeobox genes, transgenic mouse embryos were generated which contained the LacZ reporter gene under the control of different fragments from the murine Hoxb6 gene. A 2 kb limb/LPM (lateral plate mesoderm) enhancer region was identified which reproduced several aspects of the endogenous Hoxb6 gene expression pattern in the mesenchyme of the developing limb buds and the ventro-lateral body region. Both tissues represent derivatives of the lateral plate mesoderm. In order to describe proteins interacting with specific DNA sequences in the limb/LPM enhancer, electro mobility shift assays were performed using nuclear extracts prepared from embryonic tissues. Several specific DNA-protein complexes could be detected, suggesting that these DNA-protein interactions are important for the regulation of Hoxb6 gene expression.
Becker
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Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Eid
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Schughart
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D000818:Animals; D004247:DNA / Q000737:chemistry*; D004742:Enhancer Elements, Genetic; D005260:Female; D005801:Genes, Homeobox / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D004247:DNA