Comparative pharmacokinetics, distributions in tissue, and interactions with blood proteins of conventional and sterically stabilized liposomes containing 2',3'-dideoxyinosine.
The pharmacokinetics and distribution in tissue of 2',3'-dideoxyinosine (ddI) encapsulated in sterically stabilized liposomes have been evaluated in rats. Most of the sterically stabilized liposomes concentrated in the spleen with a peak level at 24 h after their intravenous injection. An extended half-life in plasma was observed for sterically stabilized liposomes (14.5 h) compared with that of conventional liposomes (3.9 h). The systemic clearance of ddI incorporated in sterically stabilized liposomes was 180 times lower than that of the free drug. The levels of in vitro and in vivo protein binding on both conventional and sterically stabilized liposomes were also evaluated. Results suggest that the amount of proteins associated with liposomes might not be the only factor involved in the in vivo clearance of liposomes, as this process may also be influenced by the nature of the bound blood proteins.
Harvie
P
P
Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, Quebec, Canada.
Désormeaux
A
A
Bergeron
M C
MC
Tremblay
M
M
Beauchamp
D
D
Poulin
L
L
Bergeron
M G
MG
D000818:Animals; D000998:Antiviral Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism*; D016049:Didanosine / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D004347:Drug Interactions; D004355:Drug Stability; D005260:Female; D007275:Injections, Intravenous; D008081:Liposomes / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D000998:Antiviral Agents; D001798:Blood Proteins; D008081:Liposomes; D016049:Didanosine