title: Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.
abstract: Chimeric mice in which lymphocytes are deficient in the Syk tyrosine kinase have been created. Compared with Syk-positive controls, mice with Syk -/- lymphocytes display substantial depletion of intraepithelial gamma delta T cells in the skin and gut, with developmental arrest occurring after antigen receptor gene rearrangement. In this dependence on Syk, subsets of intraepithelial gamma delta T cells are similar to B cells, but distinct from splenic gamma delta T cells that develop and expand in Syk-deficient mice. The characteristic associations of certain T-cell receptor V gamma/V delta gene rearrangements with specific epithelia are also disrupted by Syk deficiency.
authors.affiliation: Department of Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Mallick-Wood
authors.forename: W
authors.initials: W
authors.lastname: Pao
authors.forename: A M
authors.initials: AM
authors.lastname: Cheng
authors.forename: J M
authors.initials: JM
authors.lastname: Lewis
authors.forename: S
authors.initials: S
authors.lastname: Kulkarni
authors.forename: J B
authors.initials: JB
authors.lastname: Bolen
authors.forename: B
authors.initials: B
authors.lastname: Rowley
authors.forename: R E
authors.initials: RE
authors.lastname: Tigelaar
authors.forename: T
authors.initials: T
authors.lastname: Pawson
authors.forename: A C
authors.initials: AC
authors.lastname: Hayday
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005434:Flow Cytometry; D047908:Intracellular Signaling Peptides and Proteins; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000378:metabolism; D000072377:Syk Kinase
chemical_list: D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse