The effect of cetylpyridinium chloride (CPC) on the cell surface hydrophobicity and adherence of Candida albicans to human buccal epithelial cells in vitro.
OBJECTIVE
This study examined the effects of cetylpyridinium chloride (CPC) on cell surface hydrophobicity (CSH) and adherence of blastospores of Candida albicans (MEN strain) to human buccal epithelial cells (BEC) in vitro.


METHODS
The effect of CPC treatment of either C. albicans blastospores or BEC on their subsequent adherence was determined using 35SO4 labelled blastospores in association with a Percoll gradient. The effects of CPC treatment of blastospores on their CSH was determined using Hydrophobic Interaction Chromatography.


RESULTS
Treatment of exponential and stationary phase blastospores with CPC (50 micrograms mL-1) for 0.5-30 minutes, or with CPC (0.5-50 micrograms mL-1) for 15 minutes resulted in significant reductions in both blastospore CSH and adherence to BEC in vitro. No correlation was apparent (r < 0.8) between reduced CSH and reduced blastospore adherence following treatment with CPC (0.5-50 micrograms mL-1). Significantly reduced adherence of C. albicans (stationary or exponential growth phases) to human BEC was also observed following treatment of BEC with CPC (50 micrograms mL-1) for 0.5-30 minutes or with CPC (0.5-50 micrograms mL-1) for 15 minutes. Antiadherence effects were observed at both sub and super-minimum inhibitory concentrations of CPC.


CONCLUSIONS
It is suggested that, whilst the ability of CPC to reduce the CSH of C. albicans may contribute to its reduced adherence to human BEC in vitro, reduced CSH is only one of several possible factors that contribute to the observed antiadherence effects.
Jones
D S
DS
School of Pharmacy, University of Otago, Dunedin, New Zealand.
Schep
L J
LJ
Shepherd
M G
MG
D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D002594:Cetylpyridinium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques
D002594:Cetylpyridinium