title: Determination of some cardiovascular drugs in serum and urine by capillary isotachophoresis.
abstract: The separation and determination of amiloride, metoprolol, deacetylmetipranolol, labetalol and furosemide in human serum and urine by capillary isotachophoresis were investigated. Amiloride and beta-blockers were separated by cationic isotachophoresis in the electrolyte system sodium morpholinoethanesulfonate buffer (pH 5.5) (cL = 10 mM)-glutamic acid. Furosemide was separated using the anionic electrolyte system histidine hydrochloride buffer (pH 6.2) (cL = 10 mM)-morpholinopropanesulfonic acid. Endogenous and the possible exogenous compounds were almost totally removed from serum and urine by solid-phase extraction using a Separon SGX C18 cartridge. The recovery of compounds varied from 98.2 to 103.2%. The linearity range for the compounds was 50-1000 ng/ml. The relative standard deviations varied from 0.1 to 5.6%. The overall limits of determination ranged from 32 to 46 ng/ml of urine and from 39 to 46 ng/ml of serum, depending of the type of drugs.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Sádecká
authors.forename: J
authors.initials: J
authors.lastname: Polonský
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000652:urine; D000584:Amiloride / Q000097:blood / Q000652:urine; D002317:Cardiovascular Agents / Q000097:blood* / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000706:statistics & numerical data; D005665:Furosemide / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007741:Labetalol / Q000097:blood / Q000652:urine; D014290:Metipranolol / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D008790:Metoprolol / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results
chemical_list: D000319:Adrenergic beta-Antagonists; D002317:Cardiovascular Agents; C006204:deacetyltrimepranol; D000584:Amiloride; D005665:Furosemide; D008790:Metoprolol; D007741:Labetalol; D014290:Metipranolol