title: Blood acid-base changes during acute experimental inflammation in rats.
abstract: Acidosis has often been reported in inflamed tissues, and changes in strong relevant ions at the site of inflammation may provoke alterations in blood acid-base status. We measured changes in blood acid-base variables during carrageenan-induced inflammation in rats. We found a mixed acid-base disorder in rat blood during acute inflammation (12, 24, and 48 h). A metabolic acid contribution was found during the first 12 h and maintained further, as revealed by a decrease in plasma strong ion concentration difference ([SID]) and an increase in plasma weak acid concentration due to a rise in inorganic phosphate ([ATOT]P(i)). Plasma [SID] and [ATOT]P(i) changes were probably due to exchange of Na+ and P(i) between the inflammatory exudate and rat blood. A secondary respiratory compensation for the metabolic acid changes occurred in the blood of inflamed rats, resulting in significant hypocapnia. Furthermore, a progressive decrease in the total weak acid buffer concentration due to a decrease in plasma albumin ([ATOT]Alb) also counteracted the impact of changes in [SID] and P(i) to increase blood acidity. Therefore, despite the metabolic acid-base disorders induced by inflammatory processes, hydrogen ion (H+) homeostasis was maintained, and blood pH remained essentially unchanged in the inflamed rats.
authors.affiliation: Department of Physiology, Faculty of Biology, University of Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Alfaro
authors.forename: J
authors.initials: J
authors.lastname: Ródenas
authors.forename: L
authors.initials: L
authors.lastname: Palaclos
authors.forename: M T
authors.initials: MT
authors.lastname: Mitjavila
authors.forename: T
authors.initials: T
authors.lastname: Carbonell
mesh_terms: D000137:Acid-Base Imbalance / Q000097:blood*; D000208:Acute Disease; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D007477:Ions; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001798:Blood Proteins; D007477:Ions