Genomic structure and chromosomal mapping of the mouse VE-cadherin gene (Cdh5).
Vascular endothelial cadherin (VE-cadherin) is located strictly at endothelial junctions and appears to be a major adhesive component of cell to cell contacts. Genomic clones spanning 36 kb and encompassing the mouse VE-cadherin gene have been isolated and characterized. The gene is composed of 12 exons that exhibit conventional vertebrate splicing. The first exon is entirely untranslated, and both exons 2 and 12 contain untranslated regions. A single major transcriptional start site was identified and located 75 bases upstream of the translation initiation codon in the cDNA sequence. The proximal 5'-flanking domain lacks consensus TATA and CAAT boxes at the usual positions. Exon-intron boundaries are similar to those of other cadherin genes, with some exceptions that may have a functional significance in VE-cadherin behavior. The VE-cadherin gene (locus Cdh5) maps to mouse chromosome 8, where it colocalizes with E-cadherin (locus Cdh1), P-cadherin (locus Cdh3), and M-cadherin (locus Cdh14) genes, suggesting that it might be part of a larger cluster of cadherin sequences.
Huber
P
P
CEA, INSERM unité 217, Département de Biolgie Moléculaire et Structurale, Grenoble, France.
Dalmon
J
J
Engiles
J
J
Breviario
F
F
Gory
S
S
Siracusa
L D
LD
Buchberg
A M
AM
Dejana
E
E
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism; D005091:Exons; D005260:Female; D016678:Genome; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009115:Muridae; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D004247:DNA; D000099152:Cadherin 5