title: Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger.
abstract: The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.
authors.affiliation: Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kirimura
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: S
authors.initials: S
authors.lastname: Sugano
authors.forename: S
authors.initials: S
authors.lastname: Usami
mesh_terms: D000968:Antimycin A / Q000494:pharmacology; D001234:Aspergillus niger / Q000378:metabolism* / Q000502:physiology*; D001692:Biological Transport; D019069:Cell Respiration / Q000187:drug effects* / Q000502:physiology*; D003486:Cyanides / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004640:Emetine / Q000494:pharmacology; D006835:Hydrazones / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D012457:Salicylamides / Q000378:metabolism
chemical_list: D003486:Cyanides; D006835:Hydrazones; D012457:Salicylamides; D003609:Dactinomycin; D011691:Puromycin; D000968:Antimycin A; C005703:salicylhydroxamic acid; D003513:Cycloheximide; D010088:Oxidoreductases; D004640:Emetine