title: Molecular characterization of immune derived proenkephalin mRNA and the involvement of the adrenergic system in its expression in rat lymphoid cells.
abstract: Proenkephalin (PENK), a classically defined opioid gene, was originally thought to be expressed almost exclusively in the mature nervous and neuroendocrine systems. In the last few years, it was demonstrated, however, that significant levels of PENK mRNA and PENK-derived peptides are transiently expressed in cells of the immune system. Very little is known about the molecular mechanisms regulating this transient expression. In order to investigate those mechanisms, we examined the in vivo expression of PENK mRNA in mesenteric lymph nodes after exposing rats to lipopolysaccharide. In the present study we demonstrate that: (i) promoter usage and splicing of PENK mRNA function similarly in mesenteric lymph nodes as in neural cells; (2) PENK expression in mesenteric lymph nodes is modulated by adrenaline via adrenergic receptors; and (3) the adrenergic system participates in the modulation of the LPS induced PENK mRNA expression. These results provide more evidence for the involvement of opioids in neuro-immune interactions.
authors.affiliation: Department of Molecular Neurology, Faculty of Medicine, Hebrew University, Jerusalem, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Ovadia
authors.forename: Y
authors.initials: Y
authors.lastname: Magenheim
authors.forename: O
authors.initials: O
authors.lastname: Behar
authors.forename: H
authors.initials: H
authors.lastname: Rosen
mesh_terms: D000322:Adrenergic Agonists / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D004745:Enkephalins / Q000235:genetics*; D004837:Epinephrine / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000276:immunology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008070:Lipopolysaccharides; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology*; D009490:Neurosecretory Systems / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D000322:Adrenergic Agonists; D004745:Enkephalins; D008070:Lipopolysaccharides; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D004837:Epinephrine