title: Presence of sulphatide (3'-sulphogalactosylceramide) in pericytes in the choroid layer of the eye: sharing of this glycolipid autoantigen with islets of Langerhans.
abstract: The aim of the study was to investigate the distribution in the eye of sulphatide, an acid glycolipid which has previously been demonstrated in islets of Langerhans, nervous tissue and in kidney glomeruli of diabetic patients, and against which antibodies have been found in patients with newly diagnosed insulin-dependent diabetes mellitus. A specific monoclonal antibody, Sulph I, was used for detection of sulphatide by thin-layer chromatography, and light and electron microscope immunohistochemistry. A distinct, patchy staining was found in the choroid layer and the ciliary processes. The antigen was confirmed to be sulphatide and its concentration in human eyes was 30 nmol sulphatide/g wet tissue. By electron microscopy, anti-sulphatide choroid labelling was demonstrated in pericytes and in smooth muscle cells surrounding vessels. No Sulph I-negative pericytes were seen. Double labelling with Sulph I and anti-smooth muscle actin revealed that only pericytes in the eye contained sulphatide and not those in heart, lung, liver, adrenal, spleen, lymph node, thymus, or pancreatic tissue. Thus, sharing of the autoantigen sulphatide has been demonstrated between islets of Langerhans and pericytes in the choroid layer of the eye.
authors.affiliation: Bartholin Instituttet, Kommunehospitalet, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Buschard
authors.forename: T
authors.initials: T
authors.lastname: Horn
authors.forename: K
authors.initials: K
authors.lastname: Aaen
authors.forename: K
authors.initials: K
authors.lastname: Josefsen
authors.forename: H
authors.initials: H
authors.lastname: Persson
authors.forename: P
authors.initials: P
authors.lastname: Fredman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001324:Autoantigens / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D002196:Capillaries / Q000648:ultrastructure; D002829:Choroid / Q000276:immunology* / Q000648:ultrastructure; D004727:Endothelium / Q000648:ultrastructure; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006017:Glycolipids / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009206:Myocardium / Q000276:immunology / Q000648:ultrastructure; D010179:Pancreas / Q000276:immunology / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D011913:Rats, Inbred BB; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013433:Sulfoglycosphingolipids / Q000032:analysis / Q000737:chemistry / Q000276:immunology*
chemical_list: D001324:Autoantigens; D006017:Glycolipids; D013433:Sulfoglycosphingolipids