title: Recent progress in oligonucleotide synthesis.
abstract: New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in order to guarantee a selective cleavage of the temporary 5'-OH blocking group such as the dansylethoxycarbonyl or even the acid-labile dimethoxytrityl group in the chain elongation process. The final removal of the 2'-O-acetal function in the partially deblocked oligomer proceeds unexpectedly well under weak acidic conditions due to a supposed intramolecular acid catalysis.
authors.affiliation: Fakultät für Chemie, Universität Konstanz, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Pfleiderer
authors.forename: S
authors.initials: S
authors.lastname: Matysiak
authors.forename: F
authors.initials: F
authors.lastname: Bergmann
authors.forename: R
authors.initials: R
authors.lastname: Schnell
mesh_terms: D000080:Acetals; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis
chemical_list: D000080:Acetals; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides