title: Modulation of matrix Ca2+ content by the ADP/ATP carrier in brown adipose tissue mitochondria. Influence of membrane lipid composition.
abstract: The role of the adenine nucleotide translocase on Ca2+ homeostasis in mitochondria from brown adipose tissue was examined. It was found that in mitochondria incubated with 50 microM Ca2+, ADP was not needed to retain the cation, but it was required for strengthening the inhibitory effect of cyclosporin on membrane permeability transition as induced by menadione. In addition, carboxyatractyloside was unable to promote matrix Ca2+ release, even though it inhibits the ADP exchange reaction. However, when the Ca2+ concentration was increased to 150 microM carboxyatractyloside did induce Ca2+ release, and ADP favored Ca2+ retention. Determination of cardiolipin content in the inner membrane vesicles showed a greater concentration in brown adipose tissue mitochondria than that found in kidney mitochondria. It suggested that the failure of the adenine nucleotide translocase to influence membrane permeability transition depends on the lipid composition of the inner membrane.
authors.affiliation: Departamento de Bioquímica Instituto Nacional de Cardiología, Ignacio Chávez, México, D.F., México.
authors.forename: E
authors.initials: E
authors.lastname: Chávez
authors.forename: R
authors.initials: R
authors.lastname: Moreno-Sánchez
authors.forename: M E
authors.initials: ME
authors.lastname: Torres-Marquez
authors.forename: C
authors.initials: C
authors.lastname: Zazueta
authors.forename: C
authors.initials: C
authors.lastname: Bravo
authors.forename: S
authors.initials: S
authors.lastname: Rodríquez-Enríquez
authors.forename: C
authors.initials: C
authors.lastname: García
authors.forename: J S
authors.initials: JS
authors.lastname: Rodriguez
authors.forename: F
authors.initials: F
authors.lastname: Martinez
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008563:Membrane Lipids; D006153:Guanosine Diphosphate; D001278:Atractyloside; D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases; C003853:carboxyatractyloside; D002118:Calcium