IL-8/NAP-1 is the major T-cell chemoattractant in synovial tissues of rheumatoid arthritis.
T-cell infiltration into synovium is a crucial process for rheumatoid arthritis (RA). To investigate the mechanism of T-cell infiltration, we studied T-cell attracting activity in synovial tissue extracts of RA or osteoarthritis (OA) whose synovium lacks T-cell accumulation. RA extracts attracted twofold more T cells than OA extracts. By gel filtration column chromatography the activity of RA extracts was separated into two peaks; one was eluted at the 67-kDa region and the other was at the 12-kDa region, while the latter was absent in OA extracts. The activity eluted at the 12-kDa region was absorbed mostly by an antibody against IL-8/NAP-1, a potent T-cell chemotactic factor. IL-8/NAP-1 concentrations in RA extracts were much higher than those in OA extracts and correlated to T-cell attracting activity eluted at the 12-kDa region. The checkerboard analysis revealed that the 67-kDa activity was chemokinetic but not chemotactic. These results suggest that IL-8/NAP-1 is the major T-cell chemoattractant in RA-synovium.
Nishiura
H
H
Division of Molecular Pathology, Graduate School of Medical Sciences, Kumamoto University, Japan.
Tanaka
J
J
Takeya
M
M
Tsukano
M
M
Kambara
T
T
Imamura
T
T
D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D002630:Chemotactic Factors / Q000032:analysis* / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D016209:Interleukin-8 / Q000737:chemistry* / Q000276:immunology / Q000502:physiology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D013583:Synovial Membrane / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D002630:Chemotactic Factors; D016209:Interleukin-8