Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii.
Stewartan, the acidic exopolysaccharide of Erwinia stewartii, was purified from agar grown cells. To facilitate its structural analysis, chemical and enzymatic depolymerizations were carried out using hydrofluoric acid and E. amylovora phage phi-Ea1h, respectively. Structural characterization of the resulting oligosaccharides was performed by a combination of mass spectrometric and one- and two-dimensional (1D/2D) NMR spectroscopic techniques. A branched repeating unit with seven monosaccharide residues, all in their pyranose forms, was found: [Table: see text]
Nimtz
M
M
GBF, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Mort
A
A
Wray
V
V
Domke
T
T
Zhang
Y
Y
Coplin
D L
DL
Geider
K
K
D016667:Bacterial Capsules / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004885:Erwinia / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D009005:Monosaccharides; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C100770:stewartan