Quantitative screening for fluconazole-amphotericin B antagonism in several Candida albicans strains by a comparative agar diffusion assay.
Antagonism between fluconazole (FCZ) and amphotericin B (AMB) was determined with an agar diffusion technique using series of agar plates containing no or 10 mgl-1 FCZ (comparative diffusion assay). Serial dilutions of AMB produced concentration-dependent inhibition zones that varied between the two agar plate series. This technique served as screening method to determine FCZ-AMB interactions in 18 Candida albicans strains. The critical concentrations of AMB were enhanced 1.33- to 7.0-fold by FCZ. The critical time, T0, was reduced by half by FCZ.
Scheven
M
M
Medizinal-, Lebensmittel- und Veterinäruntersuchungsamt, Hygiene-Institut Gera, Germany.
Scheven
C
C
D000362:Agar; D000666:Amphotericin B / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000935:Antifungal Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004336:Drug Antagonism; D015725:Fluconazole / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods
D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole; D000362:Agar