Characterization of monoclonal antibodies against integrin alpha V beta 5.
A panel of monoclonal antibodies directed against the human integrin alpha V beta 5 has been generated. Mice were immunized using human alpha V beta 5 purified from placenta. Two hundred hybridoma clones were screened by ELISA and 14 were found to react with purified integrin alpha V beta 5. Here we characterize the monoclonal antibodies secreted by three clones, 14H4, 6B9, and 15F11. Two of these antibodies, 6B9 and 15F11, are highly specific for integrin alpha V beta 5. These two antibodies do not bind the highly homologous integrin alpha V beta 3. The binding of 6B9 and 15F11 to integrin alpha V beta 5 is not influenced by ligand nor by divalent cation. Antibody 14H4, obtained in the same fusion, recognizes the alpha v subunit.
Stuiver
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Program on Cell Adhesion and Extracellular Matrix, La Jolla Cancer Research Foundation, California 92037, USA.
Smith
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002413:Cations, Divalent; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000737:chemistry / Q000378:metabolism; D016023:Integrins / Q000276:immunology*; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D019030:Receptors, Vitronectin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D016023:Integrins; D008024:Ligands; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5