title: Quaternary structure dynamics and carbon monoxide binding kinetics of hemoglobin valency hybrids.
abstract: The kinetics of CO binding and changes in quaternary structure for symmetric valency hybrids of human hemoglobin have been extensively studied by laser photolysis techniques. Both alpha+beta and alpha beta+ hybrids were studied with five different ferric ligands, over a broad range of CO concentrations and photolysis levels. After full CO photolysis, the hybrid tetramers switch extensively and rapidly (< 200 microseconds) to the T quaternary structure. Both R --> T and T --> R transition rates for valency hybrid tetramers with 0 and 1 bound CO have been obtained, as well as the CO association rates for alpha and beta subunits in the R and T states. The results reveal submillisecond R reversible T interconversion, and, for the first time, the changes in quaternary rates and equilibria due to binding a single CO per tetramer have been resolved. The data also show significant alpha-beta differences in quaternary dynamics and equilibria. The allosteric constants do not vary with the spin states of the ferric subunits as predicted by the Perutz stereochemical model. For the alpha beta+CN hybrid the kinetics are heterogeneous and imply partial conversion to a T-like state with very low (seconds) R reversible T interconversion.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3125. jphilo@amgen.com
authors.forename: J S
authors.initials: JS
authors.lastname: Philo
authors.forename: U
authors.initials: U
authors.lastname: Dreyer
authors.forename: J W
authors.initials: JW
authors.lastname: Lary
mesh_terms: D000494:Allosteric Regulation; D055592:Biophysical Phenomena; D001703:Biophysics; D002248:Carbon Monoxide / Q000378:metabolism*; D004563:Electrochemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D015394:Molecular Structure; D010782:Photolysis; D011485:Protein Binding; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D006454:Hemoglobins; D008024:Ligands; D002248:Carbon Monoxide; D007501:Iron