Impact of sar and agr on methicillin resistance in Staphylococcus aureus.
The global regulators agr and sar control expression of cell wall and extracellular proteins. Inactivation of either sar and/or agr in a typical heterogeneously methicillin-resistant Staphylococcus aureus resulted in a small but reproducible decrease in the number of cells in the subpopulation expressing high methicillin resistance. The amount of low affinity penicillin-binding protein PBP2', the prerequisite for methicillin resistance, was apparently not affected, however, a reduction in PBP1 and PBP3 production was observed, suggesting that these resident PBPs of the cells might be involved somehow together with PBP2' in high level methicillin resistance.
Píriz Durán
S
S
Department of Medicine and Animal Health, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
Kayser
F H
FH
Berger-Bächi
B
B
D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006602:Hexosyltransferases; D008712:Methicillin / Q000494:pharmacology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000502:physiology; D010403:Penicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology*; D010458:Peptidyl Transferases; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*
C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C088021:SarA protein, bacterial; D015534:Trans-Activators; D014157:Transcription Factors; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin