A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia.
A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.
Wynter
C
C
School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Nathan Brisbane, Australia.
Patel
B K
BK
Bain
P
P
de Jersey
J
J
Hamilton
S
S
Inkerman
P A
PA
D001315:Australia; D001421:Bacteria, Anaerobic / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D050260:Carbohydrate Metabolism; D003910:Dextranase / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D005618:Fresh Water / Q000382:microbiology; D005798:Genes, Bacterial; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D006207:Half-Life; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003910:Dextranase