Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein.
Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma virus-specific antibodies in rabbits after immunization. Inoculations by the intradermal route protected animals against virulent RHDV and myxoma virus challenges.
Bertagnoli
S
S
Laboratoire Associé de Microbiologie Moléculaire, Institut National de la Recherche Agronomique and Ecole Nationale Vétérinaire, Toulouse, France.
Gelfi
J
J
Le Gall
G
G
Boilletot
E
E
Vautherot
J F
JF
Rasschaert
D
D
Laurent
S
S
Petit
F
F
Boucraut-Baralon
C
C
Milon
A
A
D000818:Animals; D017250:Caliciviridae Infections / Q000517:prevention & control*; D004274:DNA, Recombinant / Q000627:therapeutic use*; D004279:DNA, Viral / Q000235:genetics; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009233:Myxoma virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009234:Myxomatosis, Infectious / Q000517:prevention & control*; D011817:Rabbits; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000627:therapeutic use*; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D004274:DNA, Recombinant; D004279:DNA, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; C081149:viral protein 60, rabbit hemorrhagic disease virus