A simple thin-layer chromatographic method for the analysis of buprenorphine in urine.
A simple and sensitive (at the ng/mL level) method for buprenorphine assay in urine is described. Buprenorphine is extracted by a C18 cartridge, derivatized with dansyl chloride, resolved by a uni-dimensional, two developing mobile phase high-performance thin layer chromatography system and quantified by fluorescence densitometry at 340 nm.
Alemany
G
G
Department de Biología Fonamental i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, (Isla Baleares), Spain.
Gamundí
A
A
Rosselló
C
C
Nicolau
M C
MC
D000701:Analgesics, Opioid / Q000652:urine*; D000704:Analysis of Variance; D002047:Buprenorphine / Q000652:urine*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry; D003720:Densitometry; D005453:Fluorescence; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D003619:Dansyl Compounds; D002047:Buprenorphine; C029038:dansyl chloride