Non-steroidal anti-inflammatory agents, Part 20. Method for testing non-steroidal anti-inflammatories: the modified hen's egg chorioallantoic membrane test (HET-CAM test) compared to other procedures.
The delay of onset of irritation phenomena at the chorioallantoic membrane of incubated hen's eggs, a parameter for anti-inflammatory activity, was determined for the pharmaceutical substances diclofenac, flufenamic acid, ibuprofen, indomethacin, ketoprofen, piroxicam, phenylbutazone, salicylic acid, and sodium salicylate. Alongside questions relating to the dose-effect ratio, metabolisation, recovery, and diffusion of the substances to their site of action were investigated. The reproducibility of the procedure and its selectivity with regard to substances with a different mechanism of action is proven. The method allows classification of the substances according to their anti-inflammatory potency. However, correlation with the results of enzyme inhibition or in vivo results is only possible to a limited extent.
Dannhardt
G
G
Institut für Pharmazie, Johannes Gutenberg-Universität, Mainz, Germany.
Kreher
M
M
Nowe
U
U
Pies
A
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D002645:Chickens; D002823:Chorion / Q000378:metabolism; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004531:Eggs; D015394:Molecular Structure; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal