title: Molecular characterization and expression analysis of Manduca sexta allatotropin.
abstract: Juvenile hormones (JH) are a class of regulatory sesquiterpenoids that control metamorphosis in immature insects and reproduction in adult insects. The regulation of JH synthesis by the corpora allata (CA), a pair of endocrine glands with nervous connections to the brain, is achieved by a complex interplay of stimulatory and inhibitory factors mediated in part by the brain. The neuropeptide, allatotropin (Mas AT), was recently isolated and sequenced from the brain of the tobacco hornworm Manduca sexta. Mas AT is a 13-residue amidated peptide that activates JH synthesis in adult, but not larval, lepidopteran CA. A 23-nucleotide degenerate oligonucleotide was designed based on the peptide sequence and was used to isolate the Mas AT genomic clone. The Mas AT gene is expressed as three mRNAs which differ from one another by alternative splicing. These mRNAs are predicted to encode three distinct prohormones, each containing Mas AT. A restriction fragment from the genomic clone was then used to isolate the cDNA clone. In situ hybridization and immunohistochemistry studies show that Mas AT is expressed in both the central and enteric nervous systems. Cells expressing Mas AT were identified in the brain, frontal ganglion and terminal ganglion.
authors.affiliation: Molecular and Cellular Biology Program, Ohio University, Athens 45701, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Taylor
authors.forename: T R
authors.initials: TR
authors.lastname: Bhatt
authors.forename: F M
authors.initials: FM
authors.lastname: Horodyski
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017344:Genes, Insect; D015698:Genomic Library; D007301:Insect Hormones / Q000235:genetics* / Q000302:isolation & purification; D018402:Manduca / Q000235:genetics*; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000737:chemistry; D009479:Neuropeptides / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D007301:Insect Hormones; D009479:Neuropeptides; D011498:Protein Precursors; D012333:RNA, Messenger; C050669:allatotropin