title: Aflatoxin exposure and risk of hepatocellular carcinoma in Taiwan.
abstract: To investigate the carcinogenic effect of environmental aflatoxin exposure, 56 cases of hepatocellular carcinoma (HCC) diagnosed between 1991 and 1995 were identified and individually matched by age, sex, residence and date of recruitment to 220 healthy controls from the same large cohort in Taiwan. Blood samples were analyzed for hepatitis B and C viral markers and for aflatoxin-albumin adducts; urine was tested for aflatoxin metabolites. We obtained information about sociodemographic characteristics, habitual alcohol drinking, cigarette smoking and diet in a structured interview. Hepatitis B virus surface antigen (HBsAg) carriers had a significantly increased risk for HCC. After adjustment for HBsAg serostatus, the matched odds ratio (ORm) was significantly elevated for subjects with high levels of urinary aflatoxin metabolites. When stratified into tertiles, a dose-response relationship with HCC was observed. The ORm for detectable aflatoxin-albumin adducts was not significant after adjustment for HBsAg serostatus. HBsAg-seropositive subjects with high aflatoxin exposure had a higher risk than subjects with high aflatoxin exposure only or HBsAg seropositivity only. In male HBsAg-seropositive subjects, adjusted ORs were 2.8 (95% confidence interval [CI] = 0.9-9.1) for detectable compared with non-detectable aflatoxin-albumin adducts and 5.5 (CI = 1.3-23.4) for high compared with low urinary aflatoxin metabolite levels. Our results suggest that environmental aflatoxin exposure may enhance the hepatic carcinogenic potential of hepatitis B virus. A large-scale study will be needed to evaluate the effect of aflatoxin exposure on HBsAg non-carriers.
authors.affiliation: Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei.
authors.forename: L Y
authors.initials: LY
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Hatch
authors.forename: C J
authors.initials: CJ
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Levin
authors.forename: S L
authors.initials: SL
authors.lastname: You
authors.forename: S N
authors.initials: SN
authors.lastname: Lu
authors.forename: M H
authors.initials: MH
authors.lastname: Wu
authors.forename: W P
authors.initials: WP
authors.lastname: Wu
authors.forename: L W
authors.initials: LW
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: G T
authors.initials: GT
authors.lastname: Huang
authors.forename: P M
authors.initials: PM
authors.lastname: Yang
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
mesh_terms: D000348:Aflatoxins / Q000009:adverse effects* / Q000652:urine; D000428:Alcohol Drinking; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000821:virology; D004781:Environmental Exposure; D005260:Female; D006509:Hepatitis B; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006526:Hepatitis C; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000821:virology; D008297:Male; D012307:Risk Factors; D012709:Serum Albumin / Q000378:metabolism; D012907:Smoking; D013624:Taiwan
chemical_list: D000348:Aflatoxins; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012709:Serum Albumin