title: Intestinal mucosal permeability to 51Cr-ethylenediaminetetraacetic acid is increased after bilateral lower extremity ischemia-reperfusion in the rat.
abstract: BACKGROUND
  Despite successful revascularization of ischemic extremities, multiorgan dysfunction syndrome develops in some patients. Mechanisms responsible for this are not known; however, the gastrointestinal tract has been implicated as a possible mediator. Our objective was to demonstrate increased intestinal mucosal permeability after bilateral hindlimb ischemia-reperfusion (I-R) in a rodent model.
  
  
  METHODS
  Sixteen male Wistar rats were randomized either to 4 hours of bilateral hindlimb tourniquet ischemia and 24 hours of reperfusion (n = 8) or control groups (n = 8). The animals received 10 MuCi 51Cr-ethylenediaminetetraacetic acid (EDTA) by gavage, and excretion was measured in urine collected every 8 hours in 16 animals and every 4 hours in 8 animals. Arterial blood pressure was monitored continuously. Intravenous normal saline solution (3 ml/hr) with fentanyl (2 microgram/100 gm/hr) was continuously administered. Immediately before death complete blood count and levels of arterial lactate, creatinine, and urea were obtained. Mesenteric lymph nodes were harvested from the ileocecal region and cultured. Distal small bowel and proximal colon were preserved for histologic analysis. An additional 11 rats, six experimental and five control, were evaluated for mesenteric lymph node cultures only.
  
  
  RESULTS
  Urinary excretion of 51Cr-EDTA was significantly greater in the I-R group between 0 and 8 hours (p < 0.02) and 8 to 16 hours (p < 0.0002) of reperfusion. This increase occurred as early as 4 to 8 hours of reperfusion (p < 0.0001). Urine volume in the I-R group was significantly reduced during 0 to 4 hours of reperfusion (p < 0.002). Hemoglobin and lactate level were significantly different in the I-R group. Leukocyte and platelet counts, levels of creatinine and urea, and colony counts from mesenteric lymph nodes were similar in I-R and control groups. Blinded histologic analysis of bowel segments did not reveal morphologic differences.
  
  
  CONCLUSIONS
  Bilateral hindlimb I-R produces remote intestinal mucosal injury shown by significantly increased permeability to 51Cr-EDTA. Such increased mucosal permeability may be important in the development of multiorgan dysfunction syndrome in patients who sustain lower extremity I-R injury.
authors.affiliation: A.D. McLachlin Vascular Surgery Research Laboratory, Victoria Hospital, London, Ontario, Canada.
authors.forename: R P
authors.initials: RP
authors.lastname: Willoughby
authors.forename: K A
authors.initials: KA
authors.lastname: Harris
authors.forename: M W
authors.initials: MW
authors.lastname: Carson
authors.forename: C M
authors.initials: CM
authors.lastname: Martin
authors.forename: M
authors.initials: M
authors.lastname: Troster
authors.forename: G
authors.initials: G
authors.lastname: DeRose
authors.forename: W G
authors.initials: WG
authors.lastname: Jamieson
authors.forename: R F
authors.initials: RF
authors.lastname: Potter
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000493:pharmacokinetics*; D006614:Hindlimb / Q000098:blood supply; D007413:Intestinal Mucosa / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000378:metabolism*
chemical_list: D002860:Chromium Radioisotopes; D004492:Edetic Acid