Coeliac disease: screened by a new strategy.
The incidence of coeliac disease is different throughout Europe. The aim of our study was to investigate its true prevalence using antigliadin and endomysial antibodies in 4615 healthy adults within two different population groups in Northern Italy: 140 were positive for antigliadin antibodies (AGA), 111 only for AGA IgG, 17 for AGA IgA and 12 for IgG and IgA. Of those positive for AGA, nine had an abnormal small-intestine mucosa and were positive for endomysium antibodies (EmA) using the umbilical cord test, a novel methodology. The prevalence, 163/100,000 in the Italian group and 216/100,000 in the German group, is much higher than what was assumed. The positive predictive value of the EmA, confirmed by biopsy, is 100%, that of AGA IgG 2%, of AGA IgA 12% and of the combined AGA IgA and IgG 33%. AGA positive but EmA negative patients displayed normal histological features and distributions of alpha/beta and gamma/delta T-cell receptors on intraepithelial lymphocytes. We can therefore conclude that antigliadin antibodies are not an ideal tool for screening purposes; the umbilical cord EmA test is superior owing to its optimal sensitivity, specificity, high positive predictive value and low cost.
Pittschieler
K
K
Department of Pediatrics, Regionales Krankenhaus, Bozen, Italy.
Ladinser
B
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000097:blood; D015551:Autoimmunity; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000175:diagnosis* / Q000453:epidemiology* / Q000208:ethnology / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D005858:Germany / Q000208:ethnology; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007558:Italy / Q000453:epidemiology; D007583:Jejunum / Q000276:immunology / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D009130:Muscle, Smooth / Q000276:immunology; D011237:Predictive Value of Tests; D015995:Prevalence; D011948:Receptors, Antigen, T-Cell; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014470:Umbilical Cord / Q000276:immunology
D000906:Antibodies; D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011948:Receptors, Antigen, T-Cell; D005903:Gliadin