Triterpenoids from Pulsatilla chinensis.
A new lupane type triterpenic acid, pulsatillic acid, and two new lupane type triterpenoid glycosides, pulsatilloside A and B, along with the known 23-hydroxybetulinic acid were isolated from the roots of Pulsatilla chinensis. Their structures were characterized as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid, 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic acid 3-O-alpha-L-arabinopyranoside and 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic acid 28-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside on the basis of hydrolysis and spectral evidence including two-dimensional relay HOHAHA, one-dimensional multiple relay COSY and ROESY NMR techniques. Pulsatillic acid exhibited cytotoxic activities against P-388, Lewis lung carcinoma and human large-cell lung carcinoma.
Ye
W C
WC
Department of Phytochemistry, China Pharmaceutical University, Nanjing, China.
Ji
N N
NN
Zhao
S X
SX
Liu
J H
JH
Ye
T
T
McKervey
M A
MA
Stevenson
P
P
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D002240:Carbohydrate Sequence; D018287:Carcinoma, Large Cell; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007941:Leukemia P388; D008175:Lung Neoplasms; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010946:Plants, Medicinal; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes