Participation of the glutamatergic input of the nucleus accumbens in the regulation of the synaptic release of dopamine during associative learning.
The changes in the synaptic release of dopamine in the medial division of the nucleus accumbens in the course of a conditioned emotional response and the influence on this process of the blockade of N-methyl-D-aspartate (NMDA) receptors of this structure were investigated in awake Lister-hooded rats, using the method of intravital intracerebral dialysis in combination with high-pressure liquid chromatography and electrochemical detection. It was established that situational stimuli, previously combined with painful reinforcement, but not acoustic conditional signals, lead to a slow increase in the level of dopamine in the extracellular space of the nucleus accumbens. This process reaches a maximum 40 min after the beginning of testing and lasts 80 min. Dialysis perfusion of the nucleus accumbens with a solution of MK-801 (50 mumole/liter) does not alter the magnitude of the slow rise in the synaptic release of dopamine in this structure in the course of the conditioned emotional response, but completely blocks the late components of the release. It is concluded that the glutamatergic input of the nucleus accumbens participates in the regulation of the late components of the synaptic release of dopamine, governed by the conditioned emotional response, in this structure through NMDA receptors.
Saul'skaya
N B
NB
Laboratory of Physiology of Higher Nervous Activity, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, Saint Petersburg.
Marsden
C A
CA
D000161:Acoustic Stimulation; D000818:Animals; D001245:Association Learning / Q000502:physiology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation; D004644:Emotions / Q000502:physiology; D005106:Exploratory Behavior / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D018698:Glutamic Acid / Q000502:physiology*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009714:Nucleus Accumbens / Q000033:anatomy & histology / Q000502:physiology*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013569:Synapses / Q000378:metabolism*
D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D016291:Dizocilpine Maleate; D004298:Dopamine