Relative superiority of c-ELISA for detection of bluetongue virus antibodies.
Anti-VP7 bluetongue virus (BTV) antibodies were detected in sera of ruminant livestock using competitive enzyme linked immunosorbent assay (c-ELISA). The per cent positivity for BTV antibodies recorded in sheep was 34.61% in Haryana, 7.14% in Himachal Pradesh and 28.88% in Punjab. Goat showed 64% positivity in Haryana, 67.34% in Himachal Pradesh and 88.33% in Rajasthan whereas cattle showed 67.44% positivity in Haryana, 52.17% in Punjab and 59.15% in Rajasthan for BTV antibodies. The percent positivity recorded for buffalo was 54.05% in Punjab. A comparison of c-ELISA with dot-ELISA for a total of 181 serum samples of sheep and cattle was also done. The performance of c-ELISA relative to dot-ELISA was found to have a sensitivity of 84.61% and specificity of 89.32%.
Naresh
A
A
Department of Veterinary Microbiology, C C S Haryana Agricultural University, Hisar, India.
Prasad
G
G
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000175:diagnosis / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D002020:Buffaloes; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D006041:Goats; D012680:Sensitivity and Specificity; D012756:Sheep
D000914:Antibodies, Viral