Differential effects of interleukin-12 treatment on gene expression by allostimulated T cells from young and aged mice.
Alloantigen stimulation was used to examine the effect of interleukin (IL-12) treatment of stimulated cells from young and aged mice on the expression of mRNAs for perforin and granzyme B, two proteins known to be intimately involved in an important lytic pathway used by CTL, and mRNA for interferon (IFN)-gamma, production of which is highly stimulated by IL-12 As reported previously, IL-12 augmented the lytic activity by cells from both young and aged mice, although the relative increase was greater for the latter. The mRNAs encoding perforin and granzyme B were both marginally enhanced at early time points (for cells from young mice) or throughout the stimulation (for cells from aged mice) following allo-stimulation in the presence of IL-12. The levels of augmentation of these mRNAs was consistent with the augmentation of lytic activity. In contrast, mRNA encoding IFN-gamma was markedly enhanced throughout stimulation in cells from animals of both age groups, corresponding to the more substantial increase in interferon protein in response to IL-12.
Bloom
E T
ET
Laboratory of Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Thompson
W C
WC
Horvath-Arcidiacono
J A
JA
Burd
P R
PR
D000375:Aging / Q000276:immunology*; D000818:Animals; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D018664:Interleukin-12 / Q000494:pharmacology*; D007519:Isoantigens / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors
D007519:Isoantigens; D012333:RNA, Messenger; D018664:Interleukin-12