Role of interleukin-2 receptors in the suppressive effect of spleen cells from anti-CD3-treated mice.
In the present study, we demonstrate that unresponsive spleen T cells from mice injected with a low dose of anti-CD3 mAb (single 10 micrograms i.v. injection) significantly inhibit Con A-induced proliferation of normal spleen cells. The induction of this phenomenon requires in vivo activation since spleen cells from mice injected with the F(ab')2 fragment of anti-CD3 mAb fail to promote it. Suppression of normal T cell proliferation is concomitant with increased expression of IL-2 receptor on spleen cells from anti-CD3-treated mice. It disappears within 3 days when IL-2R has returned to background levels. A normal proliferative response to Con A can be restored when high concentrations of IL-2 are added together with the "suppressor" cells. Taken together, these data support the notion that activated spleen cells from anti-CD3-injected mice exert their inhibitory effect by competing for the IL-2 generated during culture.
Ben-Amor
A
A
CNRS URA 1461, Université René Descartes, Paris, France.
Leite-De-Moraes MdC
Lepault
F
F
Schneider
E
E
Machavoine
F
F
Arnould
A
A
Chatenoud
L
L
Dy
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D005260:Female; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000037:antagonists & inhibitors / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015375:Receptors, Interleukin-2; D003208:Concanavalin A