A new bioluminescent assay for studies of protein G and protein A binding to IgG and IgM.
It has been reported that protein G (PrG) specifically binds to IgG, but not to IgM. In the present paper, I report that using a bioluminescent immunoassay, that utilizes the recombinant calcium-dependent protein, Aequorin, as a photoprobe, IgM as well as IgG binds to PrG. The method presented here may also be used in general to study the glycosylation of proteins.
Zatta
P F
PF
Dipartimento di Biologia dell Universita di Padova, Italy. zatta@cribil.bio.unipd.it
D000331:Aequorin / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001710:Biotin / Q000031:analogs & derivatives; D002118:Calcium / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism*; D007785:Lactose / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism; D008027:Light; D008163:Luminescent Measurements; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000378:metabolism*; D019809:Streptavidin
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037102:Lectins; D013205:Staphylococcal Protein A; D000331:Aequorin; D001710:Biotin; D019809:Streptavidin; D007785:Lactose; D002118:Calcium