Regulation of hepatic apolipoprotein B RNA editing in the genetically obese Zucker rat.
Hepatic apolipoprotein (apo) B RNA editing was examined in the genetically obese hyperinsulinemic and hypertriglyceridemic Zucker rat. In obese Zucker rats, apo B RNA editing was increased 42% relative to that in lean controls. Correspondingly, the proportion of serum triglyceride-rich lipoprotein containing apo B48 increased 4.7-fold in the obese Zucker rat. Quantification of hepatic total apo B mRNA showed no difference between obese Zucker and lean control rats. In contrast, the hepatic mRNA encoding APOBEC-1, the catalytic subunit of the RNA editing activity, demonstrated an increased abundance of 1.8-fold in obese Zucker rats versus lean controls.
Phung
T L
TL
Department of Biochemistry, University of Rochester, School of Medicine and Dentistry, NY 14642, USA.
Sowden
M P
MP
Sparks
J D
JD
Sparks
C E
CE
Smith
H C
HC
D000071479:APOBEC-1 Deaminase; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D006946:Hyperinsulinism / Q000097:blood / Q000235:genetics / Q000378:metabolism; D015228:Hypertriglyceridemia / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011924:Rats, Zucker; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D001055:Apolipoproteins B; D012333:RNA, Messenger; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000071479:APOBEC-1 Deaminase; C000605091:Apobec1 protein, rat; D003564:Cytidine Deaminase