Ionizing radiation-induced apoptosis is associated with c-Jun expression and c-Jun/AP-1 activation in the developing cerebellum of the rat.
Previous studies have shown that ionizing radiation-induced cell death in the developing brain has morphological characteristics of apoptosis and is associated with internucleosomal DNA fragmentation. In the present study, we have observed c-Jun induction in cells sensitive to ionizing radiation during the whole process of radiation-induced cell death, and that this expression is accompanied by modifications in the composition of AP-1 complexes: c-Jun/AP-1 activity is highly increased whereas Jun D/AP-1 is slightly decreased. These results show that c-Jun expression and c-Jun/AP-1 activity are induced in the developing brain following ionizing radiation.
Ferrer
I
I
Unitat de Neuropatologia, Universitat de Barcelona, Spain.
Barrón
S
S
Rodríquez-Farré
E
E
Planas
A M
AM
D000818:Animals; D000918:Antibody Specificity; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects*; D001483:Base Sequence; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D004247:DNA / Q000032:analysis; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000276:immunology / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000528:radiation effects
D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D004247:DNA