Inhibition of FGF receptor activity in retinal ganglion cell axons causes errors in target recognition.
Native fibroblast growth factor receptor (FGFR) function was inhibited in developing Xenopus retinal ganglion cells (RGCs) by in vivo transfection of a dominant negative FGFR. Axons expressing the dominant negative protein advanced at 60% of the normal speed, but nevertheless navigated appropriately in the embryonic optic pathway. When they neared the optic tectum, however, many axons made erroneous turns, causing them to bypass rather than enter their target. By contrast, RGC axons expressing nonfunctional FGFR mutants entered the tectum correctly. These findings demonstrate a role for FGFR signaling in the extension and targeting of RGC axons and suggest that receptor tyrosine kinase/growth factor interactions play a critical function in establishing initial connectivity in the vertebrate visual system.
McFarlane
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Department of Biology, University of California, San Diego, La Jolla 92093-0366, USA.
Cornel
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Amaya
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Holt
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D000818:Animals; D001369:Axons / Q000737:chemistry / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D018715:Microscopy, Video; D016501:Neurites / Q000502:physiology; D017468:Receptors, Fibroblast Growth Factor / Q000037:antagonists & inhibitors* / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D015398:Signal Transduction / Q000502:physiology*; D013477:Superior Colliculi / Q000166:cytology; D014982:Xenopus laevis
D017468:Receptors, Fibroblast Growth Factor