title: Polysaccharides from the seeds of Senna multijuga.
abstract: The seeds of Senna multijuga were extracted with water or 1% acetic acid and treated with ethanol, resulting in two insoluble fractions. After purification, the major one (FIA, 23%) was shown to be a galactomannan (Man:Gal 2.3:1; [alpha] = +54.6; [eta] = 1340 ml g-1). It consists of a main chain of (1-->4)-linked beta-D-mannopyranosyl residues substituted at O6 by single-unit alpha-D-galactopyranosyl side chains. The second fraction (FIB, 2.5%) was an O-acetyl-glucuronoarabinoxylan from the seed coats (O-acetyl 8.3 mol%; glucuronic acid 11.7%, Xyl:Ara ratio 20:1), which showed a predominance of 4-O-substituted Xylp units (84.4%), branched at O3 with non-reducing end units of Xylp, Araf and glucuronic acid. The O-acetyl positions in D-xylosyl units are at O2 (4.8%), O3 (4.4%) and O2,3 (0.9%). The ratio between O3 and O2 determined by 13C-nuclear magnetic resonance spectroscopy is 1.5:1.
authors.affiliation: Department of Chemistry and Physics, Universidade de São Paulo, Brazil.
authors.forename: C G
authors.initials: CG
authors.lastname: Rechia
authors.forename: M R
authors.initials: MR
authors.lastname: Sierakowski
authors.forename: J L
authors.initials: JL
authors.lastname: Ganter
authors.forename: F
authors.initials: F
authors.lastname: Reicher
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D002850:Chromatography, Gel; D000431:Ethanol; D005690:Galactose / Q000031:analogs & derivatives; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D012676:Senna Extract / Q000737:chemistry*; D012995:Solubility; D014867:Water; D014990:Xylans / Q000302:isolation & purification*; D014994:Xylose / Q000302:isolation & purification
chemical_list: D000085:Acetates; D008351:Mannans; C099369:O-acetylglucuronoarabinoxylan; D010936:Plant Extracts; D011134:Polysaccharides; D014990:Xylans; D014867:Water; C012990:galactomannan; D000431:Ethanol; D012676:Senna Extract; D014994:Xylose; D019342:Acetic Acid; D005690:Galactose