title: Localization of reactive tyrosine residues of baker's yeast transketolase.
abstract: Baker's yeast transketolase inactivated by tetranitromethane was digested with Staphylococcus aureus V8 protease. Four peptides absorbing at 360 nm were isolated by reverse-phase HPLC and sequenced. The modified tyrosines were identified as Tyr-184, Tyr-210 and Tyr-370.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
authors.forename: M
authors.initials: M
authors.lastname: Kovina
authors.forename: M
authors.initials: M
authors.lastname: Viryasov
authors.forename: L
authors.initials: L
authors.lastname: Baratova
authors.forename: G
authors.initials: G
authors.lastname: Kochetov
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism; D014174:Transketolase / Q000737:chemistry* / Q000378:metabolism*; D014443:Tyrosine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D014443:Tyrosine; D014174:Transketolase; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase