title: Unique subcellular distribution of five annexins in resting and insulin-stimulated rat adipose cells.
abstract: Several lines of evidence suggest that annexins, a family of phospholipid-binding proteins, play a role in cellular trafficking. Five annexins (I, II, V, VI, VII) were detected in rat adipose cells. They were primarily associated with the plasma membrane in a calcium-dependent manner. None of them redistributed with insulin treatment of the cells, in contrast to the glucose transporter GLUT4, which moved from intracellular membranes to the plasma membrane. Although the actual function of annexins in adipose cells remains to be determined, our data indicate that insulin-stimulated GLUT4 trafficking does not rely on a change in subcellular location of any of the five annexins detected so far in these cells.
authors.affiliation: Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: P
authors.initials: P
authors.lastname: Raynal
authors.forename: H B
authors.initials: HB
authors.lastname: Pollard
authors.forename: S W
authors.initials: SW
authors.lastname: Cushman
authors.forename: M
authors.initials: M
authors.lastname: Guerre-Millo
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D017302:Annexins / Q000187:drug effects / Q000378:metabolism*; D004822:Epididymis; D051275:Glucose Transporter Type 4; D015151:Immunoblotting; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000378:metabolism; D009124:Muscle Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D017302:Annexins; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat