Physiological correlation between glycyrrhizin, glycyrrhizin-binding lipoxygenase and casein kinase II.
By means of glycyrrhizin (GL)-affinity column chromatography, a GL-binding lipoxygenase (gbLOX) was selectively purified from the partially purified soybean LOX-1 fraction. Polypeptide analysis of the purified gbLOX by SDS-PAGE detected two distinct polypeptides (p96 and p94), which were identical to LOX-3 as determined by their partial N-terminal amino acid sequences. Moreover, it was found that (i) phosphorylation of gpLOX by casein kinase II (CK-II) is significantly stimulated by 3 microM GL, but inhibited by 30 microM GL or 10 microM oGA; and (ii) gbLOX activity is enhanced when the enzyme is phosphorylated by CK-II in the presence of 3 microM GL. These results suggest that (i) CK-II is a kinase responsible for the activation of gbLOX through its specific phosphorylation; and (ii) GL is one of the regulatory substances for specific phosphorylation of gbLOX (LOX-3) by CK-II in plant cells.
Shimoyama
Y
Y
Kitasato University, School of Allied Health Sciences, Japan.
Ohtaka
H
H
Nagata
N
N
Munakata
H
H
Hayashi
N
N
Ohtsuki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D047390:Casein Kinase II; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D019695:Glycyrrhizic Acid; D018841:HSP90 Heat-Shock Proteins; D008084:Lipoxygenase / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D030262:Soybean Proteins
D002352:Carrier Proteins; D018841:HSP90 Heat-Shock Proteins; C490097:Hsp90b1 protein, mouse; D030262:Soybean Proteins; C086010:glycyrrhizin-binding proteins; D019695:Glycyrrhizic Acid; C490096:glycyrrhizin-binding lipoxygenase protein, Glycine max; C095864:lipoxygenase 3; D008084:Lipoxygenase; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D006034:Glycyrrhetinic Acid