title: Iron from complex salts and its bioavailability to rats.
abstract: Complex iron compounds are being used as iron suppliers. Bioavailability of their iron was tested through several parameters. A biological iron prophylactic study in rats was simultaneously carried out with three of these iron complexes products: ferric orthophosphate, iron sodium EDTA and iron glycine chelate. Their iron biodisponibility was compared to that of ferrous sulfate. Five low iron diets were prepared according to AOAC method. Four of them were enriched to a level of 20 mg Fe/kg, with ferrous sulfate and the 3 iron complexes salts under study. These diets were offered to 5 groups of 6 weanling rats each in an iron prophylactic test. Food intake was measured during 5 weeks, weight checked weekly, blood and liver collected for analyses. Weight gain, hemoglobin, hematocrit, transferrin saturation, iron hemoglobin, biodisponibility and relative iron biological values were calculated. The prophylactic iron rat assay proved to be a feasible and practical model to test and compare iron salts biodisponibility. NaFeEDTA and iron aminochelate produced similar results as ferrous sulfate and their iron has a high biological value for the rats. Orthophosphate iron had lower biological value when compared to the reference standard ferrous sulfate and the other complex products studied.
authors.affiliation: Department of Internal Medicine, São Paulo University, SP, Brazil.
authors.forename: J E
authors.initials: JE
authors.lastname: Dutra-de-Oliveira
authors.forename: M L
authors.initials: ML
authors.lastname: Freitas
authors.forename: J F
authors.initials: JF
authors.lastname: Ferreira
authors.forename: A L
authors.initials: AL
authors.lastname: Gonçalves
authors.forename: J S
authors.initials: JS
authors.lastname: Marchini
mesh_terms: D000818:Animals; D001682:Biological Availability; D004032:Diet; D004492:Edetic Acid / Q000493:pharmacokinetics; D005290:Ferric Compounds / Q000493:pharmacokinetics; D005296:Ferrous Compounds / Q000493:pharmacokinetics; D005998:Glycine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000008:administration & dosage; D058085:Iron Compounds / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014168:Transferrin / Q000378:metabolism; D014886:Weaning
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D006454:Hemoglobins; D058085:Iron Compounds; D014168:Transferrin; C101403:iron-glycine chelate; C020748:ferrous sulfate; D004492:Edetic Acid; D007501:Iron; C019179:Fe(III)-EDTA; D005998:Glycine