A porcine model of chronic peripheral arterial occlusion.
OBJECTIVE
To create a simple and reproducible model of chromic thrombosis for the evaluation of thrombolytic agents and devices.


METHODS
A stenosis was created in the superficial femoral artery of domestic swine, and autologous blood clot was deposited above the stenosis. Follow-up last for up to 3 months. Degree of clot organization was determined at histologic examination. Two thrombolytic agents, urokinase and collagenase, were used to test this model.


RESULTS
There was a 27% delayed recanalization rate with this model. At histologic examination, early thrombus organization was seen at the vessel periphery after 10 days. One-month-old thrombus was substantial but variable in amount. Three-month-old thrombus was completely organized. Neither urokinase nor collagenase proved effective against chronic clot in the doses and time course of this study.


CONCLUSIONS
This simple method yields a chronic porcine clot in a reliable number of domestic swine in 1 month.
Yoon
H C
HC
Department of Radiological Science, University of California, School of Medicine, Los Angeles 90024.
Goodwin
S C
SC
Ko
J
J
Nishimura
E
E
Rosedale
M
M
D000818:Animals; D017364:Collagenases / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005263:Femoral Artery; D010960:Plasminogen Activators / Q000627:therapeutic use*; D011859:Radiography; D013552:Swine; D015912:Thrombolytic Therapy / Q000379:methods*; D013927:Thrombosis / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000209:etiology; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000627:therapeutic use*
D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator; D017364:Collagenases