title: The identification of a novel cDNA preferentially expressed in the olfactory-limbic system of the adult rat.
abstract: To analyze cell-specific brain gene expression, we have developed a PCR-based subtractive hybridization cloning method utilizing trace starting material, allowing isolation of novel genes expressed under specific conditions. Our previous studies indicated that local substantia nigra (SN) type 1 astrocytes elaborate an array of trophic molecules which support the survival of SN dopaminergic neurons. Therefore, the current study focused on astrocyte gene expression utilizing a type 1 astrocyte-enriched cDNA library. We report initial characterization of a novel cDNA, designated AT1-46, that is preferentially expressed in the olfactory-limbic system of the adult rat brain. Although AT1-46 is expressed widely in the periphery, it is regulated both developmentally and in a cell-specific fashion in the brain. Structurally, AT1-46 is predicted to encode a highly alpha-helical molecule with several domains of potential coiled coil formation, and exhibits a 28% amino acid sequence identity with the intermediate filament-associated protein, trichohyalin.
authors.affiliation: Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635, USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Schaar
authors.forename: M R
authors.initials: MR
authors.lastname: Varia
authors.forename: S
authors.initials: S
authors.lastname: Elkabes
authors.forename: L
authors.initials: L
authors.lastname: Ramakrishnan
authors.forename: C F
authors.initials: CF
authors.lastname: Dreyfus
authors.forename: I B
authors.initials: IB
authors.lastname: Black
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D015723:Gene Library; D017403:In Situ Hybridization; D008032:Limbic System / Q000378:metabolism*; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012313:RNA