Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).
We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.
Lu
R
R
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Kanai
N
N
Bao
Y
Y
Schuster
V L
VL
D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000096:biosynthesis / Q000235:genetics*; D001692:Biological Transport; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005333:Fetus; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014018:Tissue Distribution
D017920:Antiporters; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D027361:Organic Anion Transporters; D011453:Prostaglandins; D011994:Recombinant Proteins; C503526:SLCO2A1 protein, human; C503528:Slco2a1 protein, rat