title: High sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer.
abstract: Consideration of the special problems encountered in ultra-high sensitivity biopolymer sequencing studies has led to the development of a novel quadrupole/erthogonal-acceleration time-of-flight tandem mass spectrometer described for the first time here. The performance characteristics of this new geometry are demonstrated, including fully resolved daughter-ion spectra with mass accuracies of 0.1 dalton, which allow removal of interpretation ambiguities and easy differentiation of charge states even in weak collisionally-activated decomposition tandem mass spectra. The instrument has been applied to a variety of biopolymer research problems, including the structure determination of major histocompatibility complex peptide antigens using liquid chromatography/electrospray mass spectrometry and nanoflow-electrospray tandem mass spectrometry, and sequencing capability in the low-femtomole and attomole ranges is demonstrated.
authors.affiliation: Department of Biochemistry, Imperial College, London, UK.
authors.forename: H R
authors.initials: HR
authors.lastname: Morris
authors.forename: T
authors.initials: T
authors.lastname: Paxton
authors.forename: A
authors.initials: A
authors.lastname: Dell
authors.forename: J
authors.initials: J
authors.lastname: Langhorne
authors.forename: M
authors.initials: M
authors.lastname: Berg
authors.forename: R S
authors.initials: RS
authors.lastname: Bordoli
authors.forename: J
authors.initials: J
authors.lastname: Hoyes
authors.forename: R H
authors.initials: RH
authors.lastname: Bateman
mesh_terms: D000595:Amino Acid Sequence; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*
chemical_list: D010455:Peptides