title: Double minutes in the papillary thyroid cancer cell line PTC-1113A.
abstract: The cell line PTC-1113A was established from a metastasizing recurrent papillary thyroid cancer. The cell line was growing as monolayer and showed a complex karyotype with chromosome numbers ranging from 30 to 140/metaphase. A proportion of metaphases contained double minutes and/or pulverized chromosomes. Extrachromosomal DNA seemed to originate from a B-group chromosome. A chromosome 4 painting probe hybridized to extrachromosomal material, representing double minutes (dmin) and possibly minutes. In addition, fluorescence in situ hybridization (FISH) with the chromosome 4 library detected a translocation chromosome and a pulverized chromosome originating from chromosome 4. PTC-1113A is, to our knowledge, the single papillary thyroid cancer cell line demonstrating evidence of gene amplification.
authors.affiliation: Department of Surgery and Pathology, SUNY at Stony Brook, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Herrmann
authors.forename: P A
authors.initials: PA
authors.lastname: Lalley
authors.forename: L L
authors.initials: LL
authors.lastname: Rydstedt
authors.forename: E
authors.initials: E
authors.lastname: Meese
authors.forename: C H
authors.initials: CH
authors.lastname: Lang
authors.forename: N N
authors.initials: NN
authors.lastname: Abumrad
authors.forename: U M
authors.initials: UM
authors.lastname: Moll
authors.forename: G B
authors.initials: GB
authors.lastname: Talpos
mesh_terms: D000368:Aged; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology*; D002877:Chromosomes, Human / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009362:Neoplasm Metastasis; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured