title: Effects of organic modifiers on the chiral recognition by different types of silica-immobilized bovine serum albumin.
abstract: We prepared three columns containing bovine serum albumin immobilized on silica by different means and the effects of organic modifiers in the eluent on chiral separation were studied using N-substituted amino acids. Adsorption on silica, covalent immobilization to diol-silica with carbonyldiimidazole (CSP-II) and covalent immobilization to amino-silica with glutaraldehyde were studied. CSP-II had the highest stereoselectivity and was the most affected by organic modifiers in the eluent. The hydrophobicity of amino acid moiety affected the chiral recognition of N-benzoylamino acids and the aromaticity of the N-substituted group was important.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kumanoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: Q
authors.initials: Q
authors.lastname: Yuan
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: A
authors.initials: A
authors.lastname: Sugii
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005976:Glutaral / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D007093:Imidazoles; D012710:Serum Albumin, Bovine; C006900:N,N-carbonyldiimidazole; D005976:Glutaral