Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.
Katzen
F
F
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
Becker
A
A
Zorreguieta
A
A
Pühler
A
A
Ielpi
L
L
D001483:Base Sequence; D005798:Genes, Bacterial; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D016959:Xanthomonas campestris / Q000235:genetics*
D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; C002563:xanthan gum