Antibody response to outer membrane proteins in Shigella dysenteriae type 1 infection with special reference to appearance of murine antibodies.
In shigellosis, the protective immune mechanism is not well established. The bacterial outer membrane proteins (OMPs) may have a role in the induction of immunity due to their outwardly location. The serum antibody response of S. dysenteriae type 1 infected patients against OMPs was assessed by enzyme linked immunosorbent assay (ELISA). A striking elevation of serum IgG response was noted during the convalescent phase. Murine antiserum directed against S. dysenteriae 1 OMPs was found to be highly cross reactive with the OMPs isolated from heterologous species. A major antigenic OMP was partially purified and showed distinct immunodominance in ELISA. These observations suggest that the specific component may have some immunoprophylactic potential.
Chakrabarti
S
S
Division of Immunology, National Institute of Cholera & Enteric Diseases, Calcutta.
Sinha
A K
AK
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D003429:Cross Reactions; D004405:Dysentery, Bacillary / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology; D012762:Shigella dysenteriae / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides