title: Detection of allergen-specific IgE in tears of grass pollen-allergic patients with allergic rhinoconjunctivitis.
abstract: BACKGROUND
  Allergic conjunctivitis is a common symptom amongst Type I (IgE-mediated) allergic diseases; and most frequently seen as rhinoconjunctivitis. However, the site of production and the significance of allergen specific IgE needs further elucidation.
  
  
  OBJECTIVE
  We investigated whether the presence of IgE in tears of grass pollen allergic patients correlated with disease and clinical symptoms, whether the IgE binding pattern to the different grass pollen antigens was different in sera and tears, and whether IgA antibodies to grass pollen allergens were present in tears. Finally, we looked whether specific IgE was produced locally or was exudated from serum.
  
  
  METHODS
  Sera from 44 grass pollen allergic patients suffering from either allergic rhinitis (n = 11) or allergic rhinoconjunctivitis (n = 33) and from healthy controls (n = 7) were used for the experiments. Binding of specific IgE and IgA antibodies to the different groups of grass pollen allergens (Phleum pratense) was evaluated by means of immunoblotting.
  
  
  RESULTS
  Specific IgE was detected in sera as well as in tears of allergic patients, whereby tear-derived allergen-specific IgE exerted similar specificities to the corresponding IgE from serum. The correlation between symptoms of ocular allergy and the presence of allergen-specific IgE in tears was highly significant (P < 0.0001). In contrast, only a poor correlation was found between specific and/or total IgE in sera and the manifestation of ocular allergy (P = 0.73).
  
  
  CONCLUSIONS
  Allergen-specific IgE antibodies in tears seem to be produced locally rather than exudated from serum. IgE in tears seems to be responsible for allergic conjunctivitis. IgA in tears cannot exert a protective function since the IgA antibodies recognize different antigens in a grass pollen (Phleum pratense) extract than IgE antibodies. The highly significant correlation between allergic conjunctivitis and the presence of specific tear IgE emphasizes the diagnostic value of immunoblots with tear IgE, especially in cases in which serum provides inconclusive results.
authors.affiliation: Institute of General and Experimental Pathology, University of Vienna, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Hoffmann-Sommergruber
authors.forename: E D
authors.initials: ED
authors.lastname: Ferreira
authors.forename: C
authors.initials: C
authors.lastname: Ebner
authors.forename: T
authors.initials: T
authors.lastname: Barisani
authors.forename: L
authors.initials: L
authors.lastname: Korninger
authors.forename: D
authors.initials: D
authors.lastname: Kraft
authors.forename: O
authors.initials: O
authors.lastname: Scheiner
authors.forename: I
authors.initials: I
authors.lastname: Baumgartner
mesh_terms: D000485:Allergens / Q000276:immunology*; D000918:Antibody Specificity; D003233:Conjunctivitis, Allergic / Q000097:blood / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D012621:Seasons; D013666:Tears / Q000737:chemistry / Q000276:immunology*; D014168:Transferrin / Q000032:analysis
chemical_list: D000485:Allergens; D007070:Immunoglobulin A; D014168:Transferrin; D007073:Immunoglobulin E