title: Flexilin: a new extracellular matrix glycoprotein localized on collagen fibrils.
abstract: We have immunopurified and characterized a new glycoprotein of the extracellular matrix, using a monoclonal antibody obtained after immunization with fibril-associated collagens extracted from bovine tendon. In polyacrylamide gels, the protein migrates at about 350 kDa molecular mass. The protein is insensitive to bacterial collagenase, and no disulfide-linked aggregates could be detected; sugars were stained with periodic acid-Schiff's reagent. Amino acid analysis and sequencing of tryptic peptides failed to detect any similarity with known proteins. By rotary shadowing experiments, the protein was observed as flexible, unbranched structures, approximately 150 nm long, with a small globule at one end. Investigation of the tissue distribution of the protein in fetal bovine tissues by immunofluorescence resulted in labeling in extracellular matrices with loosely packed collagen fibrils, such as the peritendineum, embryonic skin and kidney glomeruli; cornea, cartilage matrix and bone were not labeled. Ultrastructural immunolocalization in dermis and in mesangium of glomeruli showed that the protein always occurred in the vicinity of collagen fibrils. In view of its tissue distribution and molecular shape, we postulate that this protein is important in the properties of the extrafibrillar environment. By reference to its shape as observed by rotary shadowing, we propose the name 'flexilin' for this extracellular matrix glycoprotein.
authors.affiliation: Institut de Biologie et Chimie des Protéines, Université Claude Bernard, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Lethias
authors.forename: Y
authors.initials: Y
authors.lastname: Descollonges
authors.forename: M M
authors.initials: MM
authors.lastname: Boutillon
authors.forename: R
authors.initials: R
authors.lastname: Garrone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D003094:Collagen; D017364:Collagenases; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D007678:Kidney Glomerulus / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D012867:Skin / Q000166:cytology / Q000648:ultrastructure; D013710:Tendons / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D010446:Peptide Fragments; D003094:Collagen; D017364:Collagenases