title: Biosynthesis of a blood group H1 antigen by alpha 1, 2-fucosyltransferase in PC12 cells.
abstract: We have examined the expression of GDP-fucose: glycosphingolipid fucosyltransferase activity in PC12 cells and PC12 sublines in relation to the neuronal differentiation induced by nerve growth factor (NGF) or dexamethasone. Transfer of fucose to paragloboside (nLc4Cer) yielded a product which was determined to be a blood group H1 antigen (Fuc alpha 1-2Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer) by gas chromatography/mass spectrometry analysis and enzymatic hydrolysis, suggesting that PC12 cells have an alpha 1,2-fucosyltransferase. Lactosylceramide was also fucosylated at a reduced rate. When the differentiation of PC12 cells and PC12 subline cells, PC12D and MR31, was induced by exposure to either NGF or dexamethasone, the fucosyltransferase activity for nLc4Cer was found to decrease in both cell lines, suggesting the association with cell differentiation. This is the first report of the presence of an alpha 1,2-fucosyltransferase in cultured neuronal cell lines which catalyses the in vitro biosynthesis from nLc4Cer of a type-2 chain glycosphingolipid having the blood group H1 determinant. The disaccharides, beta-lactose and N-acetyllactosamine, were also fucosylated by PC12 cell enzyme, although the specificity for the carbohydrate structure was different from that for glycosphingolipids.
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kojima
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: R
authors.initials: R
authors.lastname: Mineta-Kitajima
authors.forename: Y
authors.initials: Y
authors.lastname: Sone
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000017:ABO Blood-Group System / Q000096:biosynthesis* / Q000737:chemistry; D000818:Animals; D015703:Antigens, CD; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002454:Cell Differentiation; D002855:Chromatography, Thin Layer; D003907:Dexamethasone / Q000494:pharmacology; D004187:Disaccharides / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D007790:Lactosylceramides / Q000378:metabolism; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000201:enzymology; D016716:PC12 Cells; D051381:Rats; D013379:Substrate Specificity; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000017:ABO Blood-Group System; D015703:Antigens, CD; D002250:Carbon Radioisotopes; D003907:Dexamethasone; D004187:Disaccharides; D005643:Fucose; D005647:Fucosyltransferases; D007790:Lactosylceramides; D009414:Nerve Growth Factors; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C102547:H1 blood group antigen; C009744:CDw17 antigen