Contribution of amino acids and insulin to protein anabolism during meal absorption.
The contribution of dietary amino acids and endogenous hyperinsulinemia to prandial protein anabolism still has not been established. To this end, leucine estimates ([1-14C]leucine infusion, plasma alpha-ketoisocaproic acid [KIC] specific activity [SA] as precursor pool SA) of whole-body protein kinetics and fractional secretory rates (FSRs) of albumin, fibrinogen, antithrombin III, and immunoglobulin G (IgG) were measured in three groups of healthy volunteers during intragastric infusion of water (controls, n = 5), liquid glucose-lipid-amino acid (AA) meal (meal+AA, n = 7), or isocaloric glucose-lipid meal (meal-AA, n = 7) that induced the same insulin response as the meal+AA. The results of this study demonstrate that 1) by increasing (P < 0.01) whole-body protein synthesis and decreasing (P < 0.01) proteolysis, dietary amino acids account for the largest part (approximately 90%) of postprandial protein anabolism; 2) the ingestion of an isocaloric meal deprived of amino acids exerts a modest protein anabolic effect (10% of postprandial protein anabolism) by decreasing amino acid oxidation and increasing (P < 0.01) albumin synthesis; 3) albumin FSR is increased (approximately 20%) by postprandial hyperinsulinemia (meal-AA) and additionally increased (approximately 50%) by amino acid intake (meal+AA); 4) IgG FSR is stimulated (approximately 40%) by amino acids, not by insulin; and 5) fibrinogen and antithrombin III FSR are not regulated by amino acids or insulin.
Volpi
E
E
Department of Internal Medicine, Endocrine and Metabolic Sciences, University of Perugia, Italy.
Lucidi
P
P
Cruciani
G
G
Monacchia
F
F
Reboldi
G
G
Brunetti
P
P
Bolli
G B
GB
De Feo
P
P
D000284:Administration, Oral; D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism*; D000990:Antithrombin III / Q000032:analysis; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood; D002250:Carbon Radioisotopes; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D005934:Glucagon / Q000097:blood; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D006946:Hyperinsulinism; D007074:Immunoglobulin G / Q000097:blood; D007328:Insulin / Q000097:blood*; D007408:Intestinal Absorption; D007651:Keto Acids / Q000097:blood; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000008:administration & dosage; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012709:Serum Albumin / Q000032:analysis
D000596:Amino Acids; D001786:Blood Glucose; D002096:C-Peptide; D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified; D007074:Immunoglobulin G; D007328:Insulin; D007651:Keto Acids; D008055:Lipids; D011506:Proteins; D012709:Serum Albumin; C013082:alpha-ketoisocaproic acid; D000990:Antithrombin III; D005340:Fibrinogen; D005934:Glucagon; D007930:Leucine; D005947:Glucose