The effects of altered exercise distribution on lymphocyte subpopulations.
The effects of exercise distribution on lymphocyte count, lymphocyte subpopulations and plasma cortisol concentration in peripheral blood were assessed in 19 healthy subjects. The subjects were randomly divided into group A (n = 10) or group B (n = 9) according to exercise distribution. Both groups underwent a 10-week programme involving 5 x 2-week blocks: baseline (B), training period 1 (TP1), stabilisation 1 (S1), training period 2 (TP2), and stabilisation 2 (S2). During B, S1 and S2 normal training was undertaken. During TP1 and TP2 the subjects increased the amount of training by 50% in week 1 and by 100% in week 2. During TP1 subjects in group A exercised 6 days.week-1, while during TP2 these subjects exercised on 3 alternate days.week-1, but doubled the duration of each training session. The subjects in group B reversed this training order. Blood was collected 36-42 h following exercise period B, and at the end of periods TP1, S1, TP2 and S2, and also 12-18 h following completion of exercise at the end of TP1 and TP2. There were no significant differences (P > 0.05) between the 6 day.week-1 programme and the 3 alternate day.week-1 programme in total lymphocyte count, CD3+, CD4+, CD8+, CD16+, or CD19+ cells, the CD4:CD8 ratio, HLA-DR+ (activated) T cells or plasma cortisol concentrations. Following both TP1 and TP2 there was a nonsignificant decrease in lymphocyte subpopulations. However following both S1 and S2 (baseline training) there was a significant increase in total lymphocyte count, CD3+, CD4+ and CD8+ lymphocytes. The S2 variables statistically significant from B were: total lymphocyte count (P < 0.01), CD3+ T-cells and percentage of circulating lymphocytes (P < 0.01), CD4+ cells (P < or = 0.0001), CD8+ cells (P < 0.05), and HLA-DR+ (activated) T-cells (P < 0.05). The results indicated that provided the amount of exercise is constant for a given period, then exercise distribution is not a critical variable in the alteration of lymphocyte subpopulations that may occur in response to overload training. However 2 weeks of overload training followed by 2 weeks of active recovery (baseline) training may induce an increase in the lymphocyte count.
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Faculty of Nursing, University of Sydney, Camperdown N.S.W., Australia.
Norton
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Olds
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Lowe
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Mulligan
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D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D016130:Immunophenotyping; D007694:Killer Cells, Natural; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D008297:Male
D018941:Antigens, CD19; D017252:CD3 Complex; D006854:Hydrocortisone