title: Extensive mutation scanning of RET in sporadic medullary thyroid carcinoma and of RET and VHL in sporadic pheochromocytoma reveals involvement of these genes in only a minority of cases.
abstract: Sporadic medullary thyroid carcinoma (MTC) and pheochromocytoma (PC) have been reported to be associated with some specific RET gene mutations. To assess the role of RET in the development of MTC and PC, we screened 14 sporadic MTC, two MTC-derived cell lines, and 5 sporatic PC cases of RET mutations by a systematic analysis of the whole coding sequence, including all intron-exon junctions. In only 6 of the 14 sporadic MTC we were able to detect a RET mutation. Apart from the MET918-->Thr mutation in 5 of the MTC cases, we found a 3-bp deletion in exon 11, only present in the tumor, in another case. Analysis of 2 cell lines revealed the Met918-->Thr mutation in 1 and a Cys634-->Trp mutation in the other cell line. A possible somatic nature of these mutations could not be confirmed because in neither case was constitutive DNA available. We conclude that a large proportion of sporadic MTC must be due to mutations in an unidentified gene(s) other than RET. In none of the sporadic PC cases was a RET mutation found. As PC is a frequent complication in families suffering from von Hippel Lindau disease, for which mutations of the VHL gene are responsible, we also screened the 5 sporadic PC cases for VHL mutations. This revealed a Gly164-->Ser mutation in a single specimen. Thus, in PC, a large majority of tumors are due to mutations in an unidentified gene(s) other than RET and VHL.
authors.affiliation: Department of Medical Genetics, University of Groningen, The Netherlands.
authors.forename: R M
authors.initials: RM
authors.lastname: Hofstra
authors.forename: T
authors.initials: T
authors.lastname: Stelwagen
authors.forename: R P
authors.initials: RP
authors.lastname: Stulp
authors.forename: D
authors.initials: D
authors.lastname: de Jong
authors.forename: M
authors.initials: M
authors.lastname: Hulsbeek
authors.forename: E J
authors.initials: EJ
authors.lastname: Kamsteeg
authors.forename: A
authors.initials: A
authors.lastname: van den Berg
authors.forename: R M
authors.initials: RM
authors.lastname: Landsvater
authors.forename: A
authors.initials: A
authors.lastname: Vermey
authors.forename: W M
authors.initials: WM
authors.lastname: Molenaar
authors.forename: C J
authors.initials: CJ
authors.lastname: Lips
authors.forename: C H
authors.initials: CH
authors.lastname: Buys
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis; D029721:Drosophila Proteins; D005796:Genes; D016147:Genes, Tumor Suppressor; D006801:Humans; D008025:Ligases; D010673:Pheochromocytoma / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D051794:Von Hippel-Lindau Tumor Suppressor Protein
chemical_list: D029721:Drosophila Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D051794:Von Hippel-Lindau Tumor Suppressor Protein; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila; D008025:Ligases; C497446:VHL protein, human