title: Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, Houston 77030-3303, USA.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Kan
authors.forename: W L
authors.initials: WL
authors.lastname: McKeehan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods*; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D017468:Receptors, Fibroblast Growth Factor; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases