Inhibition of replication of rinderpest virus by 5-fluorouracil.
5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%, at a concentration of 1 microgram/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.
Ghosh
A
A
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Nayak
R
R
Shaila
M S
MS
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D005472:Fluorouracil / Q000494:pharmacology*; D012333:RNA, Messenger; D012302:Rinderpest virus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000998:Antiviral Agents; D012333:RNA, Messenger; D014764:Viral Proteins; D005472:Fluorouracil