title: Effect of iron (II) on the generation of hydroxyl free radicals in rat myocardium.
abstract: We examined the effect of iron (II) on the generation of hydroxyl radicals (.OH) in the extracellular fluid of rat myocardium. Salicylic acid in Ringer's solution (0.5 nmole x microliter(-1) x min(-1)) was directly infused through a microdialysis probe to detect the generation of OH as reflected by the formation of dihydroxybenzoic acid (DHBA) in the myocardium. Iron clearly produced a dose-dependent increase in .OH formation. A positive linear correlation between iron (II) and the formation of 2,3-DHBA (R2 = 0.970) or 2.5-DHBA (R2 = 0.983) was observed. However, when desferrioxamine (DES) was infused through a dialysis probe, a marked increase in DHBA formation was obtained. The present results suggest that iron (III) may reduce .OH formation by the Fenton reaction.
authors.affiliation: Department of Pharmacology, Oita Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Obata
authors.forename: Y
authors.initials: Y
authors.lastname: Yamanaka
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects*; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017665:Hydroxyl Radical; D007501:Iron