title: Grb10: A new substrate of the insulin-like growth factor I receptor.
abstract: Using the yeast two-hybrid system, we have isolated a new substrate of the insulin-like growth factor I receptor (IGF-IR), identified as Grb10, a member of the family of SH2 domain proteins. With the help of several mutants of the IGF-IR, we have mapped the binding site of Grb10 between amino acids 1229 and 1245 of the receptor, a sequence that is dispensable for the mitogenic activity of the IGF-IR. Grb10 coprecipitates with the IGF-IR in cell lysates and is probably involved in the regulation of its activity.
authors.affiliation: Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: A
authors.initials: A
authors.lastname: Morrione
authors.forename: B
authors.initials: B
authors.lastname: Valentinis
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: J Y
authors.initials: JY
authors.lastname: Ooi
authors.forename: B
authors.initials: B
authors.lastname: Margolis
authors.forename: R
authors.initials: R
authors.lastname: Baserga
mesh_terms: D001665:Binding Sites; D051383:GRB10 Adaptor Protein; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D011506:Proteins; D017527:Receptor, IGF Type 2; D051383:GRB10 Adaptor Protein