title: Specific neutrophil hyporesponsiveness in chronic Helicobacter pylori infection.
abstract: The release from mucosal neutrophils of reactive oxygen species (ROS) is believed to be an important part of the pathogenesis of Helicobacter pylori-associated gastritis and duodenal ulcer. In this study, bacterial sonicates prepared from 1 reference and 29 patient strains were used to stimulate ROS release from peripheral blood neutrophils. In 29 H. pylori-positive patients, the neutrophil response toward the homologous strain was absent. In contrast, a significant response was observed toward the reference strain. In addition, on stimulation of neutrophils from 29 patients without H. pylori infection, ROS release was significant. The stimulatory effect on neutrophils by FMLP was comparable in the 2 groups. It is concluded that a specific neutrophil hyporesponsiveness in ROS release toward the homologous R. pylori strain exists. This feature has, to our knowledge, not been described previously for neutrophil responses to any human pathogen.
authors.affiliation: Department of Clinical Microbiology, National University Hospital, Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Nørgaard
authors.forename: L P
authors.initials: LP
authors.lastname: Andersen
authors.forename: L
authors.initials: L
authors.lastname: Elsborg
authors.forename: S
authors.initials: S
authors.lastname: Holck
authors.forename: H
authors.initials: H
authors.lastname: Nielsen
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000032:analysis; D002908:Chronic Disease; D004724:Endoscopy; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013010:Sonication
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; C080866:cagA protein, Helicobacter pylori