Nephritogenicity and alpha-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane.
Nephritogenicity (anti-GBM-nephritis-inducing activity) and alpha-chain composition of globular-do-main (NCI) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different alpha chains of type IV collagen. A purified nephritogenic fraction from renal BM contained alpha 1-alpha 6(IV)NCI, whereas a non-nephritogenic fraction contained only alpha 1-alpha 2(IV)NCI. Renal and pulmonary NCI had strong nephritogenic activity: placental NCI had weak activity. The renal and pulmonary fractions contained alpha 1-alpha 6(IV)NCI, and the placental fraction had a large amount of alpha 1-alpha 2(IV)NCI and a very small amount of alpha 3-alpha 6(IV)NCI. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained alpha 1-alpha 5(IV) chains, but not the alpha 6(IV) chain. The absence of alpha 6(IV) chain in glomerular BM in bovine and in humans indicates that alpha 6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of alpha 3-alpha 5(IV)NCI.
Rauf
S
S
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
Kagawa
M
M
Kishiro
Y
Y
Inoue
S
S
Naito
I
I
Oohashi
T
T
Sugimoto
M
M
Ninomiya
Y
Y
Sado
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D001485:Basement Membrane / Q000737:chemistry*; D015153:Blotting, Western; D002417:Cattle; D003094:Collagen / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005921:Glomerulonephritis / Q000276:immunology* / Q000473:pathology; D007074:Immunoglobulin G / Q000032:analysis; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000473:pathology; D008168:Lung / Q000737:chemistry; D051381:Rats; D011921:Rats, Inbred WKY
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D007074:Immunoglobulin G; D003094:Collagen