title: Regulation of cell volume in a human biliary cell line: activation of K+ and Cl- currents.
abstract: The mechanisms responsible for recovery from cell swelling were evaluated in Mz-ChA-1 cells from human cholangiocarcinoma, a model biliary cell line. Exposure to hypotonic buffer (40% less NaCl) rapidly increased relative cell volume to 1.35 +/- 0.10 as measured by a Coulter Multisizer, followed by regulatory volume decrease to 1.08 +/- 0.03 by 30 min. The same maneuver increased 86Rb (69 +/- 17%) and 125I (422 +/- 58%) efflux in cell monolayers. 86Rb efflux was selectively inhibited by Ba2+ [half-maximal inhibitory concentration (IC50) approximately 1.5 mM], and 125I by 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) (IC60 approximately 50 microM). Inhibition of these conductive pathways partially inhibited recovery from swelling. Membrane conductance measured by whole cell patch-clamp analysis increased in 57 of 57 cells during swelling due to activation of both K+ and Cl- conductances in most cells. K+ currents (75% of cells, 881 +/- 150 pA at 0 mV) were nearly linear and Ba2+ sensitive; Cl- currents (70% of cells, 2,696 +/- 244 pA at +60 mV) were outwardly rectified, showed time-dependent inactivation at depolarizing potentials, and were inhibited by NPPB. Chelation of cytosolic Ca2+ decreased swelling-induced isotope efflux, prevented activation of macroscopic K+ and Cl- currents, and blocked volume recovery. These studies indicate that biliary cells are able to regulate cell volume during osmotic stress by activation of separate K+ and Cl- conductances through a mechanism that depends in part on Ca(2+)-sensitive signaling pathways.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Roman
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J G
authors.initials: JG
authors.lastname: Fitz
mesh_terms: D001652:Bile Ducts / Q000166:cytology* / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology*; D004553:Electric Conductivity; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D009994:Osmolar Concentration; D009995:Osmosis; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D018118:Chloride Channels; D015221:Potassium Channels; D002118:Calcium