In vitro effect of interleukin-12 on antigen-specific lymphocyte proliferative responses from persons infected with human immunodeficiency virus type 1.
The relationship between CD4 lymphocyte count and the in vitro effect of interleukin (IL)-12 on lymphocyte proliferative responses to Candida, tetanus toxoid, and streptokinase antigens was studied in peripheral blood mononuclear cells (PBMC from 30 human immunodeficiency virus (HIV)-infected persons and 10 seronegative controls. IL-12 significantly increased proliferative responses to microbial recall antigens of PBMC from HIV-infected persons with >200 CD4 lymphocytes/mm3 but had little effect on PBMC from patients with more advanced disease. The greatest increase was seen in patients with 200-500 CD4 cells/mm3. Results of limiting dilution analysis suggested that the increase in antigen-specific lymphocyte proliferation in the presence of IL-12 was due to an increase in the number of responding cells rather than an increase in the extent of proliferation of a fixed number of responder cells.
Uherova
P
P
Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver 80262, USA.
Connick
E
E
MaWhinney
S
S
Schlichtemeier
R
R
Schooley
R T
RT
Kuritzkes
D R
DR
D000942:Antigens, Bacterial / Q000276:immunology; D018791:CD4 Lymphocyte Count; D002175:Candida / Q000276:immunology; D002478:Cells, Cultured; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000254:growth & development; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013300:Streptokinase / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D000942:Antigens, Bacterial; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D013745:Tetanus Toxoid; D018664:Interleukin-12; D013300:Streptokinase