Effect of hepatocyte growth factor on radiation response of HeLa, V79, CHO and primary cultured parenchymal hepatocyte in vitro.
Hepatocyte growth factor (HGF) is a multipotent cytokine enhancing regeneration of injured organs as liver, kidney and lung after injury. HGF enhances proliferation of various type of cells, inhibits proliferation of carcinoma cells, enhances motility of epithelial cells. We examined three cell lines (CHO, HeLa, V79) and primary cultured normal rat parenchymal hepatocytes to determine the effect of HGF on radiation response. HGF diminished survival of CHO and V79 cells determined by colony formation assay, whereas no significant change of survival was found in HeLa cells. No synergistic changes of survival were found when these three cell lines were irradiated with the addition of HGF. Thus, HGF did not enhance the radiation effect. We also analyzed the impact of irradiation with HGF on primary cultured normal rat parenchymal hepatocytes. At first, the release of glutamic-oxaloacetic amino-transaminase (GOT) in the supernatant was estimated. Irradiation (40 Gy) with or without HGF did not change GOT release in acute phase by 4 days after irradiation compared with the unirradiated control. Second, the DNA synthesis of rat parenchymal hepatocytes was analyzed using radioactive iodine-labeled deoxyuridine incorporation. HGF counteracted the suppression of DNA synthesis induced by irradiation. Thus, HGF may act as a mitogen even for irradiation-damaged normal cells.
Yamazaki
H
H
Department of Radiation Oncology, Osaka University Medical School, Japan.
Inoue
T
T
Nose
T
T
Murayama
S
S
Teshima
T
T
Ozeki
S
S
Koizumi
M
M
Inoue
T
T
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D016466:CHO Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D004622:Embryo, Mammalian; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D011836:Radiation Tolerance / Q000187:drug effects; D051381:Rats
D017228:Hepatocyte Growth Factor; D004247:DNA; D001219:Aspartate Aminotransferases