Isolation of tetranucleotide repeat polymorphisms flanking the BRCA1 gene.
Large pools of cosmids from the BRCA1 region of human chromosome 17 were screened for tetranucleotide repeat polymorphisms by hybridizing shotgun subcloned pools with a mixture of 25 oligonucleotides. Identified subclones were PCR amplified and directly sequenced to design PCR primers for short tandem repeat polymorphism (STRP) analysis of family DNAs. With the identification of the BRCA1 gene and the observation that most mutations in this > 100-kb gene are unique, haplotyping and linkage analysis may play a significant role in diagnosis and carrier detection of BRCA1-associated breast and ovarian cancers. We report the characterization of 15 new STRPs flanking the BRCA1 locus.
Bennett-Baker
P E
PE
Department of Human Genetics, Human Genome Center, University of Michigan Medical School, Ann Arbor 48109-0618, USA.
Kiousis
S
S
Chandrasekharappa
S C
SC
King
S E
SE
Abel
K J
KJ
Collins
F S
FS
Weber
B L
BL
Chamberlain
J S
JS
D019313:BRCA1 Protein; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014157:Transcription Factors / Q000235:genetics*
D019313:BRCA1 Protein; D017931:DNA Primers; D009363:Neoplasm Proteins; D014157:Transcription Factors