title: cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.
abstract: We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter.
authors.affiliation: Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Vernet
authors.forename: J
authors.initials: J
authors.lastname: Cebrian
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D005260:Female; D005333:Fetus / Q000821:virology*; D009052:Leukemia Virus, Murine / Q000502:physiology*; D051379:Mice; D010957:Plasmids; D011247:Pregnancy; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics*