title: Heterogeneity in the appearance and distribution of macrophage subsets and their possible involvement in hypertensive vascular lesions in rats.
abstract: In hypertensive vascular lesions, various pathologic changes are exhibited. To clarify the mechanisms responsible for this diversity of vascular lesions, we immunohistochemically examined hypertensive vascular lesions in stroke-prone spontaneously hypertensive rats with reference to the distribution of macrophage subsets. The brain, kidney, heart, and aorta were dissected from stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats at 8, 12, 16, and 20 weeks of age. Immunohistochemistry was performed with antibodies against the macrophage markers ED1, ED2, and OX42, the MHC class II antigen marker OX6, the T-lymphocyte markers CD4, CD5, and CD8, and other markers, such as alpha-smooth muscle actin, proliferating cell nuclear antigen, and von Willebrand factor. Fibrinoid necrosis was dominant in the brain, and fibrocellular proliferative lesions were dominant in the kidney. Immunohistochemically, the decreased intensity of alpha-smooth muscle actin immunostaining preceded the formation of vascular lesions. Although preexisting ED2-positive perivascular resident macrophages completely disappeared in fibrinoid necrosis, MHC class II-negative and ED1-positive macrophages were scattered around the lesion and showed phagocytosis in the brain, which indicates that macrophages in fibrinoid necrosis had extravasated and acted only as scavengers. In the kidney, there was extensive accumulation of MHC class II-positive and ED1-positive macrophages with T lymphocytes along the affected arteries. These inflammatory cells seemed to be supplied through the perivascular interstitial space, not through the endothelium, and the accumulation of these cells preceded the development of fibrocellular proliferative lesions. In the heart and aorta, macrophage accumulation was either absent or slight, and vascular lesions were rarely observed. These findings suggest that heterogeneity in the time course of macrophage infiltration and in the distribution of macrophage subsets among the vascular trees of various organs seems to be correlated with the diversity of hypertensive vascular lesions. Differences in the routes that supply macrophages and their functions may determine the pathologic changes in the vascular lesions.
authors.affiliation: National Cardiovascular Center Research Institute, Kinki University School of Medicine, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Abumiya
authors.forename: J
authors.initials: J
authors.lastname: Masuda
authors.forename: J
authors.initials: J
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: J
authors.initials: J
authors.lastname: Ogata
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D001921:Brain / Q000098:blood supply / Q000473:pathology; D003331:Coronary Vessels / Q000473:pathology; D006973:Hypertension / Q000473:pathology*; D007668:Kidney / Q000098:blood supply / Q000473:pathology; D008264:Macrophages / Q000145:classification / Q000473:pathology*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013045:Species Specificity; D014652:Vascular Diseases / Q000209:etiology* / Q000473:pathology*