title: [Lymph node biopsy].
abstract: Lymph node biopsy is often easy to perform but final histologic diagnosis and accuracy depends on its technical quality: adequate biopsy and proper fixation and processing. This technical note defines advice on the selection of the lymph node biopsy site, shows the interest of a previous lymph node needle aspiration and gives the principles of histopathological techniques: cytology on imprints from the excised fresh unfixed node, fixation, staining methods ad immunohistochemistry. A place is given to the indications of frozen section diagnosis, frozen tissue, electron microscopy, microbiological, cytogenetic and molecular studies for gene rearrangement.
authors.affiliation: Service d'Anatomie et de Cytologie Pathologiques, Hôpital Necker Enfants Malades, Paris.
authors.forename: N
authors.initials: N
authors.lastname: Brousse
mesh_terms: D001706:Biopsy; D015925:Cryopreservation; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008198:Lymph Nodes / Q000473:pathology*; D011237:Predictive Value of Tests
chemical_list: D005404:Fixatives
vernacular_title: La biopsie ganglionnaire.