Anti-GM1 ganglioside antibodies cloned from autoimmune neuropathy patients show diverse binding patterns in the rodent nervous system.
We have recently cloned a panel os monoclonal IgM anti-GM1 ganglioside antibodies from peripheral blood lymphocytes of patients with multifocal motor neuropathy and Guillain Barré syndrome. In solid-phase immunoassay, the antibodies all reacted with GM1 and also reacted to different degrees with the structurally related glycolipids asialo-GM1 and GD1b. These antibodies are being used to study the pathogenesis of the anti-GM1 antibody-medicated neuropathy in different experimental systems. In the present immunofluorescence study we report the binding patterns of 5 of these antibodies in the rodent nervous system. The antibodies demonstrated highly diverse binding patterns on tissue sections and teased fibers when compared to one another and between species. The antibodies bound many central and peripheral nervous system structures, including neurons and myelin, motor end plate regions, and muscle spindles. The diversity of binding shown by these antibodies provide evidence that may account for the differing clinical phenotypes, including normality, associated with elevated titers of anti-GM1 antibodies.
O'Hanlon
G M
GM
University Department of Neurology, Insitute of Neurological Sciences, Southern General Hospital, Glasgow, G51 4TF, Scotland.
Paterson
G J
GJ
Wilson
G
G
Doyle
D
D
McHardie
P
P
Willison
H J
HJ
D000818:Animals; D003164:Competitive Bidding; D005677:G(M1) Ganglioside / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D016472:Motor Neuron Disease / Q000276:immunology / Q000378:metabolism*; D009420:Nervous System / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005677:G(M1) Ganglioside