Analysis of charged cyclomalto-oligosaccharides (cyclodextrin) derivatives by ion-spray, matrix-assisted laser-desorption/ionization time-of-flight and fast-atom bombardment mass spectrometry, and by capillary electrophoresis.
The use of electrospray-ionization mass spectrometry (ESIMS), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDITOFMS), and fast-atom bombardment mass spectrometry (FABMS) for the rapid determination of molecular weight, degree of substitution (ds), and purity is demonstrated for charged derivatives of cyclomalto-heptaose (beta CD) and -octaose (gamma CD). The access to anionic sulfoalkyl ethers (alkyl: ethyl, n-propyl, and n-butyl) and to beta CD-2-hydroxy-3-trimethylammoniumpropyl ether chlorides (HTAP-beta CD) leads only to mixtures of products, the compositions of which can be determined directly from ESI and MALDITOF mass spectra. All charged derivatives consist of a mixture of unreacted and higher substituted compounds. The substitution patterns obtained by MS are in good agreement with the results of experiments on the separation of beta CD-sulfoalkyl ethers by capillary electrophoresis (CE).
Chankvetadze
B
B
Institut für Pharmazeutische Chemie der Universität, Münster, Germany.
Endresz
G
G
Blaschke
G
G
Juza
M
M
Jakubetz
H
H
Schurig
V
V
D002197:Capillary Action; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002942:Circular Dichroism; D003505:Cyclodextrins / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D003505:Cyclodextrins