Production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus.
The L1 major capsid protein of human papillomavirus type 45 was expressed in insect cells using recombinant baculovirus technology. Human papillomavirus type 45 L1 major capsid protein self-assembled into empty virus-like particles (VLPs). These 50-60 nm diameter particles were present in the nuclei of recombinant baculovirus-infected insect cells and had a density of 1.29 - 1.30 g/cm3 in cesium chloride. The expressed human papillomavirus type 45 L1 protein sequence is identical to the reference human papillomavirus type 45 strain except for one amino acid located at position 49 of the human papillomavirus type 45 L1 protein. It must be noted that the quantity of purified human papillomavirus type 45 virus-like particles is at a lower level than that previously observed with human papillomavirus type 16. Nevertheless, the ability to generate preparative amounts of human papillomavirus type 45 virus-like particles is of great importance for the production of an anti-human papillomavirus vaccine.
Touzé
A
A
Laboratoire d'Immunologie des Maladies Infectieuses, Faculté de Pharmacie, Tours, France.
Dupuy
C
C
Chabaud
M
M
Le Cann
P
P
Coursaget
P
P
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017924:Nucleopolyhedroviruses / Q000235:genetics; D027383:Papillomaviridae / Q000502:physiology*; D017354:Point Mutation; D012689:Sequence Homology, Nucleic Acid; D018411:Spodoptera; D014771:Virion / Q000648:ultrastructure; D019065:Virus Assembly / Q000502:physiology*