title: Salivary EGF regulates eosinophil-derived TGF-alpha expression in hamster oral wounds.
abstract: Using hamster as an oral wound healing model, we examined eosinophils and their expression of transforming growth factor-alpha (TGF-alpha) and transforming growth factor-beta 1 (TGF-beta 1). Oral wounds healed approximately two times faster than their cutaneous counterparts. Eosinophils infiltrated prominently into oral wounds; however, unlike the dual expression of TGF-alpha and TGF-beta 1 in skin wounds, oral wound-associated eosinophils expressed TGF-beta 1, but not TGF-alpha. Because saliva is present in oral environments and contains epidermal growth factor (EGF) and TGF-alpha, sialoadenectomy was performed in this model to determine whether the lack of TGF-alpha expression by eosinophils in oral wounds is due to the presence of salivary EGF and/or TGF-alpha. We found that eosinophils in sialoadenectomized hamsters did express TGF-alpha during oral wound healing but that such expression was suppressed when EGF was added to their drinking water. Taken together, our findings suggest that eosinophil-derived TGF-alpha and salivary TGF-alpha/ EGF may have complementary roles in contributing to TGF-alpha in oral wound healing.
authors.affiliation: Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, Boston, Massachusetts 02215, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: L W
authors.initials: LW
authors.lastname: Tyler
authors.forename: R B
authors.initials: RB
authors.lastname: Donoff
authors.forename: B
authors.initials: B
authors.lastname: Song
authors.forename: A J
authors.initials: AJ
authors.lastname: Torio
authors.forename: G T
authors.initials: GT
authors.lastname: Gallagher
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: A
authors.initials: A
authors.lastname: Elovic
authors.forename: J
authors.initials: J
authors.lastname: McBride
authors.forename: C M
authors.initials: CM
authors.lastname: Yung
authors.forename: S J
authors.initials: SJ
authors.lastname: Galli
authors.forename: P F
authors.initials: PF
authors.lastname: Weller
authors.forename: D T
authors.initials: DT
authors.lastname: Wong
mesh_terms: D000818:Animals; D006224:Cricetinae; D004804:Eosinophils / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000502:physiology*; D008297:Male; D009061:Mouth Mucosa / Q000293:injuries*; D012333:RNA, Messenger / Q000378:metabolism; D012463:Saliva / Q000378:metabolism*; D012469:Salivary Glands / Q000502:physiology; D013997:Time Factors; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor