Identification of chiral drug isomers by capillary electrophoresis.
Separation of optical isomers of compounds of pharmaceutical interest by capillary electrophoretic techniques is reviewed. The direct and indirect separation method, as well as the main resolution mechanisms and the parameters influencing the stereoselectivity are discussed considering capillary zone electrophoresis, micellar electrokinetic chromatography, isotachophoresis and electrochromatography. Several chiral selectors have been successfully used in CE for chiral separation, including cyclodextrins and their derivatives, modified crown-ethers, proteins, antibiotics, linear saccharides and chiral surfactants. Only applications in the pharmaceutical field with the most important experimental conditions are summarised in the Tables reported in this paper. The chiral analyses of drugs in real samples like biological fluids or pharmaceutical formulations are also reported.
Fanali
S
S
Istituto di Cromatografia del C.N.R., Area della Ricerca di Roma, Italy.
D001826:Body Fluids / Q000737:chemistry; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism; D013678:Technology, Pharmaceutical
D003505:Cyclodextrins; D004364:Pharmaceutical Preparations