title: Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
abstract: The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.
authors.affiliation: Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Katzen
authors.forename: A
authors.initials: A
authors.lastname: Becker
authors.forename: A
authors.initials: A
authors.lastname: Zorreguieta
authors.forename: A
authors.initials: A
authors.lastname: Pühler
authors.forename: L
authors.initials: L
authors.lastname: Ielpi
mesh_terms: D001483:Base Sequence; D005798:Genes, Bacterial; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D016959:Xanthomonas campestris / Q000235:genetics*
chemical_list: D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; C002563:xanthan gum