Suppressing effects of 6-(2,5-dichlorophenyl)-2,4-diamino-1,3,5-triazine and related synthetic compounds on azoxymethane-induced aberrant crypt foci in rat colon.
The modifying effects of dietary administration of 6-(2,5-dichlorophenyl)-2,4-diamino-1,3,5-triazine and 5 related compounds on the occurrence of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in rats. Male F344 rats were given s.c. injections of AOM (15 mg/kg body weight) once a week for 3 weeks to induce ACF. They also received the diet containing 200 ppm test compound for 5 weeks, starting one week before the first dosing of AOM. At the termination of experiment, all of the compounds had caused a significant reduction in ACF frequency, which might be associated with suppression of the expression of proliferation biomarkers. The apoptotic index in the colonic mucosal epithelium of rats killed at 6 h after the first AOM exposure revealed no blocking activity of the compounds.
Hirose
Y
Y
First Department of Pathology, Gifu University School of Medicine, Japan.
Tanaka
T
T
Makita
H
H
Yang
M
M
Satoh
K
K
Hara
A
A
Maeda
M
M
Toriyama
H B
HB
Mori
H
H
Tsuda
H
H
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis; D001397:Azoxymethane; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D004032:Diet; D004848:Epithelium / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000097:blood; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D014227:Triazines / Q000008:administration & dosage / Q000627:therapeutic use*
D016588:Anticarcinogenic Agents; D011073:Polyamines; D014227:Triazines; D009955:Ornithine Decarboxylase; D001397:Azoxymethane; C041836:irsogladine