title: KAP-1, a novel corepressor for the highly conserved KRAB repression domain.
abstract: The KRAB repression domain is one of the most widely distributed transcriptional effector domains yet identified, but its mechanism of repression is unknown. We have cloned a corepressor, KAP-1, which associates with the KRAB domain but not with KRAB mutants that have lost repression activity. KAP-1 can enhance KRAB-mediated repression and is a repressor when directly tethered to DNA. KAP-1 contains a RING finger, B boxes, and a PHD finger; the RING-B1-B2 structure is required for KRAB binding and corepression. We propose that KAP-1 may be a universal corepressor for the large family of KRAB domain-containing transcription factors.
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Friedman
authors.forename: W J
authors.initials: WJ
authors.lastname: Fredericks
authors.forename: D E
authors.initials: DE
authors.lastname: Jensen
authors.forename: D W
authors.initials: DW
authors.lastname: Speicher
authors.forename: X P
authors.initials: XP
authors.lastname: Huang
authors.forename: E G
authors.initials: EG
authors.lastname: Neilson
authors.forename: F J
authors.initials: FJ
authors.lastname: Rauscher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D003852:Deoxyribonucleoproteins / Q000737:chemistry; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000378:metabolism; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D000076183:Tripartite Motif-Containing Protein 28; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D003852:Deoxyribonucleoproteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D000072041:PAX3 Transcription Factor; C489409:PAX3 protein, human; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C068686:Pax3 protein, mouse; C103190:Trim28 protein, mouse; D000076183:Tripartite Motif-Containing Protein 28