Acceptor specificity of cyclodextrin glucanotransferase from an alkalophilic Bacillus species and synthesis of glucosyl rhamnose.
Cyclodextrin glucanotransferase [1,4-alpha-D-glucan 4-alpha-D-(1,4-alpha-D-glucano)-transferase (cyclizing), EC 2. 4. 1. 19] from an alkalophilic Bacillus species A2-5a had a wider acceptor specificity than that from B. macerans, which was similar to those from B. stearothermophilus and B. circulans. Glucosyl rhamnose produced by the CGTase was identified as glucopyranosyl-alpha-1,4-rhamnopyranose by alpha- and beta-glucosidase treatments, and 1H- and 13C-NMR analyses.
Kometani
T
T
Biochemical Research Laboratory, Ezaki Glico Co. Ltd., Osaka, Japan.
Terada
Y
Y
Nishimura
T
T
Nakae
T
T
Takii
H
H
Okada
S
S
D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004187:Disaccharides; C103234:glucopyranosyl-alpha-L-rhamnopyranose; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase