Influence of cephalosporins on intestinal enzymatic activity.
The effect of the broad spectrum antibiotics cefaclor, cefadroxil, cephradine, cefatrizine, cephaloglycine and cefroxadine was examined on rat intestinal brush border enzymes, aminopeptidase N (E.C. 3.4.11-2), dipeptidyl peptidase IV (E.C. 3.4.14.5) and alkaline phosphatase (E.C. 1.3.1.3.). All the cephalosporins assayed -except cefaclor- inhibit the aminopeptidase N activity, in an uncompetitive manner. Cefatrizine showed the most important inhibitory effect (52.5%; p < 0.001). Cefaclor and cefadroxil have no effect on the activity of the dipeptidyl peptidase IV, while cephaloglycine and cephradine showed a non competitive type inhibition. In contrast, cefatrizine and cefroxadine showed a competitive inhibition for this enzyme. None of the cephalosporins assayed had any effect on alkaline phosphatase activity.
Jordán
J
J
Departamento de Fisiología y Nutrición, Facultad de Farmacia, Universidad de Navarra, Pamplona, Spain.
Idoate
I
I
Urdaneta
E
E
Larralde
J
J
D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D018826:CD13 Antigens / Q000187:drug effects; D002511:Cephalosporins / Q000494:pharmacology*; D018819:Dipeptidyl Peptidase 4 / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar
D002511:Cephalosporins; D000469:Alkaline Phosphatase; D018826:CD13 Antigens; D018819:Dipeptidyl Peptidase 4