Muscarinic receptor subtypes in subpopulations of human blood mononuclear cells as analyzed by RT-PCR technique.
In this study we have analysed the expression of mRNA encoding the m1-m5 mAChR subtypes in human blood mononuclear cells and subpopulations of lymphocytes using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Total RNA was extracted from human blood mononuclear cells. T cells, monocytes. EB virus transformed B cells and from two leukemic cell lines and analysed by RT-PCR. Our results indicate that mRNAs for the m3, m4 and m5 muscarinic subtypes are expressed in mononuclear cells and purified T cells while m1 and m2 mRNAs were not detected in these cells. No m1-m5 subtype mRNA was detected in B cells and monocytes, indicating absence of muscarinic receptors in these cells. The expression of muscarinic subtypes in the leukemic T cell line. Peer, and the promyelocytic leukemic cell line HL-60 was different from peripheral mononuclear cells. Both m3 and m5 subtypes were expressed in Peer cells but not the m4 subtype, whereas the m4 and m5 subtypes detected in HL-60 cells.
Hellström-Lindahl
E
E
Department of Clinical Neuroscience and Family Medicine, Karolinska Institutet, Huddinge University Hospital, Sweden.
Nordberg
A
A
D001402:B-Lymphocytes / Q000166:cytology / Q000821:virology; D001483:Base Sequence; D015870:Gene Expression / Q000276:immunology; D018922:HL-60 Cells / Q000737:chemistry / Q000502:physiology; D004854:Herpesvirus 4, Human; D006801:Humans; D007938:Leukemia; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000502:physiology*; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000502:physiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011976:Receptors, Muscarinic / Q000235:genetics*; D014170:Transformation, Genetic
D012333:RNA, Messenger; D011976:Receptors, Muscarinic