Herpesvirus Saimiri encodes a new cytokine, IL-17, which binds to a novel cytokine receptor.
Herpesvirus Saimiri gene 13 (HVS13) exhibits 57% identity with the predicted sequence of a T cell-derived molecule termed CTLA8. Recombinant HVS13 and CTLA8 stimulate transcriptional factor NF-kappa B activity and interleukin-6 (IL-6) secretion in fibroblasts, and costimulate T cell proliferation. An HVS13.Fc fusion protein was used to isolate a cDNA encoding a novel receptor that also binds CTLA8. This receptor is unrelated to previously identified cytokine receptor families. A recombinant soluble receptor inhibited T cell proliferation and IL-2 production induced by PHA, concanavalin A (conA), and anti-TCR MAb. These results define CTLA8 and HVS13 as novel cytokines that bind to a novel cytokine receptor. We propose to call these molecules IL-17, vIL-17, and IL-17R, respectively.
Yao
Z
Z
Immunex Corporation, Seattle, Washington 98101, USA.
Fanslow
W C
WC
Seldin
M F
MF
Rousseau
A M
AM
Painter
S L
SL
Comeau
M R
MR
Cohen
J I
JI
Spriggs
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000276:immunology*; D020381:Interleukin-17; D007378:Interleukins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D018121:Receptors, Cytokine / Q000276:immunology / Q000302:isolation & purification*; D011971:Receptors, Immunologic / Q000276:immunology / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000956:Antigens, Viral; D020381:Interleukin-17; D007378:Interleukins; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins