Procoagulant albumin increases vascular endothelial cell prostacyclin secretion.
Vascular endothelium regulates multiple aspects of platelet function through secretion of a variety of substances, including von Willebrand factor, nitric oxide, and prostacyclin (PGI2). The objective of this study was to determine whether procoagulant albumin (P-A1), a modified form of albumin present in normal human plasma could modulate endothelial cell secretion of these substances. P-A1 did not affect constitutive secretion of von Willebrand factor or nitric oxide, but did increase PGI2 secretion in a time- and concentration-dependent manner. Pre-treatment of endothelial cells with aspirin, or use of suramin, a broad-specificity inhibitor, prevented the response to P-A1. Prostaglandin H synthase-2 contributed to the P-A1-induced PGI2 secretion. These results indicate that in addition to inducing tissue factor activity and reducing protein C activation and fibrinolysis, P-A1 also modulates vascular endothelial cell PGI2 secretion, and potentially, platelet function.
Gubler
D B
DB
Department of Medicine, University of Utah, Salt Lake City 84123, USA.
Ahlstrom
C R
CR
Liu
L
L
Zhou
J F
JF
Parker
C J
CJ
Rodgers
G M
GM
D001241:Aspirin / Q000494:pharmacology; D001665:Binding Sites; D001779:Blood Coagulation Factors / Q000187:drug effects / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000502:physiology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012709:Serum Albumin / Q000187:drug effects / Q000494:pharmacology*; D013498:Suramin / Q000494:pharmacology; D013925:Thromboplastin / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000378:metabolism
D001779:Blood Coagulation Factors; D010975:Platelet Aggregation Inhibitors; D012709:Serum Albumin; D014841:von Willebrand Factor; D009569:Nitric Oxide; D013498:Suramin; D013925:Thromboplastin; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases; D001241:Aspirin