The active transport of polypeptides in the rabbit nasal mucosa is supported by a specific vesicular transport inhibited by cytochalasin D.
We have previously demonstrated that polypeptides (elcatonin and ACTH) can be actively absorbed across the rabbit nasal mucosa. In this paper we show that elcatonin is also transported when it is adsorbed onto microspheres (diameter: 0.5 micron). whereas the elcatonin-uncovered microspheres do not display any net transport. Cytochalasin D (0.1 microgram/ml) abolishes the net absorption of elcatonin presented either alone or adsorbed. At the same concentration the inhibitor does not affect cellular active ion transports (and hence metabolism); although it increases intercellular ion and elcatonin permeability, it does not affect intercellular and paracellular permeability of the elcatonin-covered microspheres. Altogether, these results show that polypeptide transport is supported by a specific vesicular transfer inhibited by cytochalasin D by disassembly of the actin cytoskeleton, probably at the apical border of the cell.
Cremaschi
D
D
Dipartimento di Fisiologia c Biochimica Generali, Università degli Studi di Milano, Italy.
Porta
C
C
Ghirardelli
R
R
D000042:Absorption; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism; D008297:Male; D008863:Microspheres; D009297:Nasal Mucosa / Q000378:metabolism*; D010539:Permeability; D011817:Rabbits
D015638:Cytochalasin D; D000324:Adrenocorticotropic Hormone; D002116:Calcitonin; C012755:elcatonin