Construction of a restriction map and gene map of the lettuce chloroplast small single-copy region using Southern cross-hybridization.
The small single-copy region (SSCR) of the chloroplast genome of many higher plants typically contain ndh genes encoding proteins that share homology with subunits of the respiratory-chain reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase complex of mitochondria. A map of the lettuce chloroplast SSCR has been determined by Southern cross-hybridization, taking advantage of the high degree of homology between a tobacco small single-copy fragment and a corresponding lettuce chloroplast fragment. The gene order of the SSCR of lettuce and tobacco chloroplasts is similar. The cross-hybridization method can rapidly create a primary gene map of unknown chloroplast fragments, thus providing detailed information of the localization and arrangement of genes and conserved open reading frame regions.
Mitchelson
K R
KR
University of Aberdeen, UK. krm@forbio.com.au
D015139:Blotting, Southern / Q000379:methods*; D002736:Chloroplasts / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant; D018745:Genome, Plant; D018545:Lactuca / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010947:Plants, Toxic; D015183:Restriction Mapping; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics
D018744:DNA, Plant