Two different approaches for developing immunometric assays of haptens.
To improve immunoassays of small haptens, we developed two different approaches for their measurement in a non-competitive format. We first devised two-site immunometric assays for small peptides (8-11 amino acids) by selecting two sets of antibodies specifically directed against C- and N-terminal moieties of the peptides. In each case, assay sensitivity improved substantially over that of the corresponding competitive assays. More interestingly, all of these new immunometric assays were much more specific than the competitive assays. In a second approach, we developed a new procedure, solid-phase-immobilized epitope immunoassay (SPIE-IA), in which a single monoclonal antibody uses the same epitope for capture and tracer binding and the hapten is covalently cross-linked to solid-phase proteins. To date, SPIE-IA have been successfully applied to the determination of haptens bearing primary amino groups, including substance P, thyroxine, leukotriene C4, endothelin, and angiotensin II. In each case, assay sensitivity was significantly improved.
Grassi
J
J
CEA, Service de Pharmacologie et d'Immunologie, Gif sur Yvette, France.grassi@dsvidf.cea.fr
Créminon
C
C
Frobert
Y
Y
Etienne
E
E
Ezan
E
E
Volland
H
H
Pradelles
P
P
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D000939:Epitopes / Q000032:analysis; D006241:Haptens / Q000032:analysis*; D007118:Immunoassay; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D015288:Neurokinin A / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D051381:Rats; D012680:Sensitivity and Specificity; D013373:Substance P / Q000032:analysis
D000939:Epitopes; D006241:Haptens; D010455:Peptides; D000804:Angiotensin II; D013373:Substance P; D015288:Neurokinin A