title: Control of beef spoilage by a sulfide-producing Lactobacillus sake strain with bacteriocinogenic Leuconostoc gelidum UAL187 during anaerobic storage at 2 degrees C.
abstract: Chill-stored, vacuum-packaged beef inoculated with sulfide-producing Lactobacillus sake 1218 developed a distinct sulfide odor within 3 weeks of storage at 2 degrees C, at which time the bacteria had reached maximum numbers of 10(6) CFU cm(-2). Coinoculation of the meat with the wild-type, bacteriocinogenic (Bac+) strain of Leuconostoc gelidum UAL187 delayed the spoilage by L. sake 1218 for up to 8 weeks of storage. Coinoculation of meat samples with an isogenic, slowly growing Bac+ variant, UAL187-22, or with the Bac- variant UAL187-13 did not delay the onset of spoilage by L. sake 1218. The study showed that spoilage of chill-stored, vacuum-packaged beef by a susceptible target organism could be dramatically delayed by the Bac+ wild-type strain of L. gelidum UAL187. Inoculation with L. sake 1218 can be used as a model system to determine the efficacy of biopreservation of vacuum-packaged meats.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: J J
authors.initials: JJ
authors.lastname: Leisner
authors.forename: G G
authors.initials: GG
authors.lastname: Greer
authors.forename: M E
authors.initials: ME
authors.lastname: Stiles
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis*; D002417:Cattle; D003080:Cold Temperature; D005069:Evaluation Studies as Topic; D018857:Food Packaging; D005519:Food Preservation / Q000379:methods*; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D007934:Leuconostoc / Q000254:growth & development / Q000378:metabolism*; D008460:Meat / Q000382:microbiology*; D013440:Sulfides / Q000378:metabolism*; D014618:Vacuum
chemical_list: D001430:Bacteriocins; D013440:Sulfides