title: Kinetics of in vivo degradation of sepiolite-collagen complexes: effect of glutaraldehyde treatment.
abstract: A kinetic study of the in vivo degradation of sepiolite-collagen complexes after subcutaneous implantation in rats was performed. A foreign-body reaction was the characteristic host tissue response against the implants. The resorption of the implanted materials was analyzed by measuring both the weight and the collagen persistences. This last was measured by using (14C)acetylated collagen, which was revealed to be not modified upon radioactive labeling, in terms of its ability to form a complex with sepiolite. The persistence of the implants is controllable by treatment of the collagen component with glutaraldehyde. Thus, for 1% glutaraldehyde-treated collagen complexes, 100% of persistence was observed after several months of implantation, this value decreasing to a few days for nontreated collagen samples. The collagen-sepiolite complex showed a low immunological response, almost null for 1% glutaraldehyde-treated collagen complexes, which was analyzed by measuring anti-collagen antibodies levels. Based on the performed studies, sepiolite-collagen complexes can be considered a resorbable material.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad Complutense, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Olmo
authors.forename: J
authors.initials: J
authors.lastname: Turnay
authors.forename: J I
authors.initials: JI
authors.lastname: Herrera
authors.forename: J G
authors.initials: JG
authors.lastname: Gavilanes
authors.forename: M A
authors.initials: MA
authors.lastname: Lizarbe
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D002942:Circular Dichroism; D003094:Collagen / Q000009:adverse effects / Q000737:chemistry* / Q000276:immunology; D017364:Collagenases; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005549:Foreign-Body Reaction / Q000276:immunology; D005976:Glutaral / Q000009:adverse effects / Q000737:chemistry* / Q000276:immunology; D006868:Hydrolysis; D007700:Kinetics; D017633:Magnesium Silicates / Q000009:adverse effects / Q000737:chemistry* / Q000276:immunology; D008297:Male; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D017633:Magnesium Silicates; D003094:Collagen; C001671:magnesium trisilicate; D017364:Collagenases; D005976:Glutaral