Rapid detection of chemical carcinogens by measuring RNA released from microsomes in post-mitochondrial supernatant.
Incubation of carcinogens with post-mitochondrial supernatant (PMS) and NADPH releases ribosomes from microsomes resulting in increased RNA concentration in post-microsomal supernatant. However, non-carcinogens fail to do so. Enhanced concentration of RNA in test over control samples can provide a useful index for the carcinogenicity of environmental pollutants.
Dhanda
J S
JS
Department of Biochemistry, Panjab University, Chandigarh, India.
Dani
H M
HM
Sharma
R
R
D000818:Animals; D002273:Carcinogens / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D012313:RNA / Q000032:analysis*; D051381:Rats; D013997:Time Factors
D002273:Carcinogens; D012313:RNA