Longitudinal analysis of human salivary immunoglobulins, nonimmune antimicrobial agents, and microflora after tonsillectomy.
In order to study the role of tonsils in the host defense in the oral region one pre- and two postoperative (1 and 6 months) whole saliva samples were collected from 25 young adults referred for tonsillectomy. Saliva samples were analyzed for selected host defense factors, representing both immune (total IgA, IgG, IgM, anti-Streptococcus mutans, anti-EBV, anti-CMV, and anti-adenovirus IgA and IgG) and nonimmunoglobulin (lysozyme, lactoferrin, salivary peroxidases, thiocyanate, hypothiocyanite, and agglutinins) mediators. Following tonsillectomy, a significant (P < 0.04) reduction was observed in specific IgG antibodies, suggesting that tonsils participate in local IgG response to oral antigens. Total IgM levels also decreased (P< 0.006), which may to some extent reflect reduced antigenic stimuli compared to preoperative status with frequent tonsillitis. Saliva-derived nonimmunoglobulin host defense factors, except lactoferrin, which declined significantly, remained normal throughout the study period. Our study indicates that tonsils play a role in local oral IgG-mediated immune response but tonsillectomy does not seem to lead to any significant long-term impairment of salivary defense capacity.
Kirstilä
V
V
Turku Immunology Centre, University of Turku, Finland.
Tenovuo
J
J
Ruuskanen
O
O
Suonpää
J
J
Meurman
O
O
Vilja
P
P
D000293:Adolescent; D000328:Adult; D000373:Agglutinins / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D008137:Longitudinal Studies; D008297:Male; D009113:Muramidase / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011184:Postoperative Period; D012463:Saliva / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology*; D013295:Streptococcus mutans / Q000276:immunology; D013861:Thiocyanates / Q000032:analysis; D014068:Tonsillectomy
D000373:Agglutinins; D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D013861:Thiocyanates; C014607:hypothiocyanite ion; D009195:Peroxidase; D009113:Muramidase