Ascorbate-stimulated lipid peroxidation in human brain is dependent on iron but not on hydroxyl radical.
Lipid peroxidation has been postulated as a cause of neuronal damage in both aging and neurodegenerative disease. In studies of neurodegenerative disease, iron, iron plus ascorbate, or ascorbate alone has been used to stimulate lipid peroxidation. The mechanism by which ascorbate stimulates lipid peroxidation in human brain is unclear. The studies reported here were performed to determine whether ascorbate-stimulated lipid peroxidation in human brain membrane fragments was dependent on iron, superoxide radical, hydrogen peroxide, or hydroxyl radical. Our findings indicate that although ascorbate-stimulated lipid peroxidation in brain is iron dependent, it apparently does not require hydrogen peroxide or hydroxyl radical, but rather is dependent on superoxide radical formation.
Andorn
A C
AC
Department of Psychiatry, St. Louis University School of Medicine and St. Louis VAMC, Missouri, USA.
Britton
R S
RS
Bacon
B R
BR
D000328:Adult; D001205:Ascorbic Acid / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002421:Caudate Nucleus / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism; D008875:Middle Aged; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism
D002614:Chelating Agents; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D017665:Hydroxyl Radical; D008353:Mannitol; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D003676:Deferoxamine; D001205:Ascorbic Acid