title: Molecular cloning and sequencing of cDNAs encoding insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Simon).
abstract: Plectreurys tristis cephalothorax mRNA was isolated and amplified by PCR using degenerate primers corresponding to reverse translated mature Plt-VI toxin. An oligonucleotide corresponding to a portion of the amplified product was then used to screen a P. tristis cDNA library. The cDNAs from 10 positive clones were sequenced. Eight of these cDNAs corresponded to Plt-VI toxin, one to Plt-XI toxin, and one was very similar to Plt-VIII toxin, with the exception of a single amino acid substitution. Analysis of these cDNAs indicated that these toxins are initially synthesized as prepro-forms which undergo signal cleavage followed by additional processing at both their N- and C-termini to produce the mature products.
authors.affiliation: Sandoz Agro Inc., Palo Alto, CA 94304, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Leisy
authors.forename: J D
authors.initials: JD
authors.lastname: Mattson
authors.forename: G B
authors.initials: GB
authors.lastname: Quistad
authors.forename: S J
authors.initials: SJ
authors.lastname: Kramer
authors.forename: N
authors.initials: N
authors.lastname: Van Beek
authors.forename: L W
authors.initials: LW
authors.lastname: Tsai
authors.forename: F E
authors.initials: FE
authors.lastname: Enderlin
authors.forename: A R
authors.initials: AR
authors.lastname: Woodworth
authors.forename: M E
authors.initials: ME
authors.lastname: Digan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007306:Insecticides; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013112:Spiders / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D007306:Insecticides; D010455:Peptides; D012333:RNA, Messenger