Effect of degree of unsaturation in dietary fatty acids on arachidonic acid mobilization by peritoneal macrophages.
Cells from rats fed with a tripalmitin diet showed a depletion of phospholipid arachidonate and n-3 fatty acids such as eicosapentaenoic and docosahexaenoic acids (EPA and DHA). In rats fed fish oil diet, a significant reduction in arachidonic acid (AA) content was observed whereas EPA and DHA were incorporated into membranes lipids. These changes in lipid composition of membranes did not affect cellular adherence, phagocytic capability, or [3H]AA incorporation. However, both tripalmitin and fish oil diets induced a decrease in [3H]AA mobilization stimulated by 4 beta-phorbol-12-myristate 13-acetate, A23187, or opsonized-zymosan in rat peritoneal macrophages. These results demonstrate that the antiinflammatory effects of essential fatty acids deficiency or n-3 enrichment diets may be associated with a decreased AA mobilization in resident rat peritoneal macrophages treated with proinflammatory agents.
Mitjavila
M T
MT
Departamento de Fisiología, Facultad de Biología, Universidad de Barcelona, Spain.
Rodríguez
M C
MC
Sáiz
M P
MP
Lloret
S
S
Moreno
J J
JJ
D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry* / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000737:chemistry* / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009895:Opsonin Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014280:Triglycerides / Q000008:administration & dosage; D015054:Zymosan / Q000494:pharmacology
D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D009895:Opsonin Proteins; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D000001:Calcimycin; D015054:Zymosan; D015118:Eicosapentaenoic Acid; C005859:tripalmitin; D013755:Tetradecanoylphorbol Acetate