Amino acid sequence determination of human S100A12 (P6, calgranulin C, CGRP, CAAF1) by tandem mass spectrometry.
S100A12 has been isolated from human neutrophils. The molecular weight and the amino acid sequence of S100A12 was determined by electrospray-mass spectrometry, tandem mass spectrometry and Edman microsequence analysis. Interestingly, a sequence comparison of S100A12 with all known human S100 proteins revealed that S100A12 is the most divergent of the S100 proteins.
Ilg
E C
EC
Department of Pediatrics, University of Zurich, Switzerland.
Troxler
H
H
Bürgisser
D M
DM
Kuster
T
T
Markert
M
M
Guignard
F
F
Hunziker
P
P
Birchler
N
N
Heizmann
C W
CW
D000595:Amino Acid Sequence; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D009418:S100 Proteins; D000067616:S100A12 Protein; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human; D015740:Calcitonin Gene-Related Peptide