Strategies for expressing human antibody repertoires in transgenic mice.
Repertoires of human antibodies can be created in transgenic mice carrying human immunoglobulin-gene loci in germline configuration. These 'transloci', introduced either as miniloci or as almost locus-sized regions, undergo rearrangement and hypermutation in mouse lymphoid tissue. Here, Marianne Brüggemann and Michael Neuberger review the use of such mice for raising antigen-specific human monoclonal antibodies, as well as their exploitation for studying regulatory aspects of antibody repertoire formation.
Brüggemann
M
M
Dept of Development and Genetics, Babraham Institute, Cambridge, UK.
Neuberger
M S
MS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins