title: Enhanced excretion of intermediates of aromatic amino acid catabolism during chlorophenol degradation due to nutrient limitation in the yeast Candida maltosa.
abstract: Incubation of phenol-induced cells of the yeast Candida maltosa SBUG 700 with mono- and dichlorophenols resulted in the formation of metabolites of the substrates and of further metabolites not related to the degradation pathway of the substrates. These additional compounds, identified as 4-hydroxyphenylacetic acid (4-HPA), phenylacetic acid (PA), indolylacetic acid (IA) and indolylethanol (i.e.) by means of HPLC and GC/MS, were not excreted in incubation experiments with glucose. The excretion of these metabolites of aromatic amino acid metabolism is not caused by toxic effects of the phenol derivatives, but seems to be a result of carbon and nitrogen starvation of yeast cells.
authors.affiliation: Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany. hammer@microbiol.biologie.uni-greifsweld.de
authors.forename: E
authors.initials: E
authors.lastname: Hammer
authors.forename: H
authors.initials: H
authors.lastname: Kneifel
authors.forename: K
authors.initials: K
authors.lastname: Hofmann
authors.forename: F
authors.initials: F
authors.lastname: Schauer
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D010648:Phenylacetates / Q000032:analysis*
chemical_list: D000596:Amino Acids; D017077:Culture Media, Conditioned; D010636:Phenols; D010648:Phenylacetates; D019800:Phenol; C025136:phenylacetic acid; D005947:Glucose