Cross-reactivities of polyclonal antibodies against lebetase, fibrinolytic enzyme of Levantine viper (Vipera lebetina) venom.
Antibodies to two fractions of fibrinolytic enzyme in Vipera lebetina venom were produced by immunizing mice with chromatographically purified lebetase probes. The antibodies were isolated from mice blood by protein A affinity chromatography. The antibodies to both fractions reacted only with the fibrinolytic enzyme in V. lebetina venom as demonstrated by western immunoblotting. Immunodot assays, ELISA and western immunoblotting revealed that 11 snake venoms including species of Viperidae and Crotalidae but not Elapidae cross-react with lebetase antibodies to varying degrees. The molecular weights of cross-reacting components were detected and compared with the earlier published data.
Siigur
J
J
Department of Bioorganic Chemistry, Estonian Academy of Sciences, Tallinn, Estonia.
Tõnismägi
K
K
Tu
A T
AT
Siigur
E
E
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D008666:Metalloendopeptidases / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D014757:Viper Venoms / Q000201:enzymology*
D000906:Antibodies; D014757:Viper Venoms; D008666:Metalloendopeptidases; C069853:lebetase