Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain.
The cytosolic cAMP activates in eukaryotic cells several isoforms of cAMP-dependent protein kinase (PKAs) involved in signal transduction. The effects of individual PKA isoforms are determined by their cellular localisation, specified through binding to distinct A Kinase Anchor Proteins (AKAPs). A new member of the AKAP family, a membrane-anchored 903 amino acid long protein, designated AKAP149, is characterized in the present work. It is a putative splicing variant of S-AKAP84 with the important new feature of a RNA-binding motif (KH domain). This domain together with the known characteristics of AKAPs suggests the involvement of AKAP149 in the phosphorylation-dependent regulation of RNA-processing.
Trendelenburg
G
G
Department of Gastroenterology, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Germany.
Hummel
M
M
Riecken
E O
EO
Hanski
C
C
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000906:Antibodies; D001665:Binding Sites; D015139:Blotting, Southern; D015153:Blotting, Western; D002352:Carrier Proteins; D003106:Colon; D016384:Consensus Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D054758:A Kinase Anchor Proteins; C105137:AKAP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000906:Antibodies; D002352:Carrier Proteins; D015342:DNA Probes; D008565:Membrane Proteins; D010446:Peptide Fragments; D016601:RNA-Binding Proteins; D017868:Cyclic AMP-Dependent Protein Kinases