[Lymph node biopsy].
Lymph node biopsy is often easy to perform but final histologic diagnosis and accuracy depends on its technical quality: adequate biopsy and proper fixation and processing. This technical note defines advice on the selection of the lymph node biopsy site, shows the interest of a previous lymph node needle aspiration and gives the principles of histopathological techniques: cytology on imprints from the excised fresh unfixed node, fixation, staining methods ad immunohistochemistry. A place is given to the indications of frozen section diagnosis, frozen tissue, electron microscopy, microbiological, cytogenetic and molecular studies for gene rearrangement.
Brousse
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N
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Necker Enfants Malades, Paris.
D001706:Biopsy; D015925:Cryopreservation; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008198:Lymph Nodes / Q000473:pathology*; D011237:Predictive Value of Tests
D005404:Fixatives
La biopsie ganglionnaire.