title: Observer variation in AgNOR counts in neoplastic breast lesions.
abstract: OBJECTIVE
  To determine interobserver and intraobserver variability of AgNOR quantitation in neoplastic lesions of the breast.
  
  
  METHODS
  Forty-five cases, 20 benign and 25 malignant lesions, were included in the study. Counts were performed on one slide from each case within a pre-marked area of about 1 cm2 in a standardized manner by two observers blind to the histopathologic diagnosis and independent of each other and repeated after two weeks. Interobserver and intraobserver agreement was assessed using the Bland-Altman method.
  
  
  RESULTS
  Our results showed small mean interobserver and intraobserver differences but wide limits of agreement.
  
  
  CONCLUSIONS
  Observer variation in AgNOR counts is too high for the method to be of any diagnostic or prognostic relevance.
authors.affiliation: Department of Pathology, King George's Medical College, Lucknow, India.
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
authors.forename: P K
authors.initials: PK
authors.lastname: Agarwal
authors.forename: M M
authors.initials: MM
authors.lastname: Goel
authors.forename: S
authors.initials: S
authors.lastname: Tandon
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: R P
authors.initials: RP
authors.lastname: Misra
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis* / Q000453:epidemiology*; D005260:Female; D018226:Fibroadenoma / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D009697:Nucleolus Organizer Region / Q000473:pathology*; D015588:Observer Variation; D010212:Papilloma / Q000175:diagnosis* / Q000453:epidemiology*; D016622:Silver Staining / Q000706:statistics & numerical data*