Recipient-reactive antibodies occur during development of acute graft-versus-host reaction after small bowel transplantation.
After small bowel transplantation, not only rejection but also graft-versus-host reaction (GVHR) may occur. Donor T lymphocytes, transferred together with the graft, are a prerequisite for the development of GVHR. So far, however, little is known about the effector mechanisms in acute GVHR. It can be assumed that not only T lymphocytes but also other cells, i.e., B lymphocytes, monocytes/macrophages, and NK cells, together with inflammatory cytokines, are responsible for the lesions of recipient tissue. In the present study, the occurrence of recipient-reactive antibodies after semisyngeneic small bowel transplantation was investigated to elucidate the role of B lymphocytes in GVHR development. No antibodies reactive with recipient cells were detectable in serum from untreated, nontransplanted rats. Five days after transplantation, recipient-reactive antibodies started to appear in recipient serum. At the same time, a deposition of IgM antibodies became visible in the liver and native intestine, which are target organs for GVHR. No antibodies directed against either the donor strain or a third-party strain were detectable in serum. We conclude that a synthesis of antibodies against recipient tissue occurs during the development of GVHR. Whether these antibodies contribute to the disease remains unclear.
Johnsson
C
C
Department of Transplantation Surgery, University Hospital, Uppsala, Sweden.
Bengtsson
M
M
Tufveson
G
G
D000208:Acute Disease; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D006087:Graft vs Host Reaction / Q000276:immunology*; D006824:Hybridization, Genetic; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007421:Intestine, Small / Q000637:transplantation*; D008099:Liver / Q000276:immunology; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew
D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M