title: Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: an immunohistochemical study in the rat.
abstract: An antibody which recognizes specifically a metabotropic glutamate receptor, mGluR7, was produced by using a trpE fusion protein containing a C-terminal sequence of rat mGluR7. Neuropil in laminae I and II of the dorsal horn of the rat, as well as many neuronal cell bodies in the dorsal root ganglion, showed mGluR7-like immunoreactivity; the immunoreactivity in neuropil was seen in axon terminals, which were filled with round synaptic vesicles and constituted axodendritic and axosomatic asymmetric synapses. The mGluR7-like immunoreactivity in laminae I and II in the dorsal horn was reduced after dorsal rhizotomy. The results indicate that some axon terminals of the primary afferent fibers to laminae I and II of the dorsal horn are provided with mGluR7.
authors.affiliation: Department of Morphological Brain Science, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohishi
authors.forename: S
authors.initials: S
authors.lastname: Nomura
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Ding
authors.forename: R
authors.initials: R
authors.lastname: Shigemoto
authors.forename: E
authors.initials: E
authors.lastname: Wada
authors.forename: A
authors.initials: A
authors.lastname: Kinoshita
authors.forename: J L
authors.initials: JL
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Neki
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D005727:Ganglia, Spinal / Q000737:chemistry / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009475:Neurons, Afferent / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis* / Q000276:immunology; D017661:Receptors, Presynaptic / Q000032:analysis* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013116:Spinal Cord / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D018094:Receptors, Metabotropic Glutamate; D017661:Receptors, Presynaptic; D011993:Recombinant Fusion Proteins