title: Reovirus type 1 associated with meningitis.
abstract: A previously healthy 3-month-old girl presented with symptoms of meningitis, diarrhoea, vomiting and fever. Green monkey kidney (GMK) cells inoculated with cerebrospinal fluid (CSF) revealed reovirus-like particles by electron microscopy. RNA-gel electrophoresis, immunofluorescence and virus neutralization identified the pathogen isolated from CSF as reovirus type 1. Antibody determination by immunofluorescence of paired sera showed a significant antibody titre rise to the CSF isolate and neutralization test revealed a greater than 4-fold antibody titre rise to the CSF isolate. The CSF isolate was also neutralized by reovirus type 1 antibodies. This report represents one of a few associating reovirus with CNS symptoms in humans.
authors.affiliation: Department of Medical Microbiology, University Hospital of Lund, Sweden.
authors.forename: P J
authors.initials: PJ
authors.lastname: Johansson
authors.forename: T
authors.initials: T
authors.lastname: Sveger
authors.forename: K
authors.initials: K
authors.lastname: Ahlfors
authors.forename: J
authors.initials: J
authors.lastname: Ekstrand
authors.forename: L
authors.initials: L
authors.lastname: Svensson
mesh_terms: D003937:Diagnosis, Differential; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D008587:Meningitis, Viral / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000503:physiopathology; D008854:Microscopy, Electron; D012087:Reoviridae / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000503:physiopathology