title: In situ detection of the Epstein-Barr virus-encoded nuclear antigen 1 in oral hairy leukoplakia and virus-associated carcinomas.
abstract: A new monoclonal antibody has been used to examine immunohistochemically the expression of the Epstein-Barr virus (EBV)-encoded nuclear antigen (EBNA) 1 in virus-associated epithelial lesions. EBNA1 was detected in the tumour cell nuclei of 10/13 undifferentiated nasopharyngeal carcinomas and of 10/10 EBV-associated gastric carcinomas. EBNA1 was also detected in 13 of 16 oral hairy leukoplakia (HL) samples, where its expression was confined to nuclei in the upper epithelial cell layers whilst basal epithelial cells were negative. This observation is in agreement with previous studies demonstrating the absence of latent EBV infection in the basal cell compartment of HL and suggests an essential role for EBNA1, not only in latent EBV infection but also in virus replication.
authors.affiliation: Institute for Cancer Studies, University of Birmingham, U.K.
authors.forename: P G
authors.initials: PG
authors.lastname: Murray
authors.forename: G
authors.initials: G
authors.lastname: Niedobitek
authors.forename: E
authors.initials: E
authors.lastname: Kremmer
authors.forename: F
authors.initials: F
authors.lastname: Grässer
authors.forename: G M
authors.initials: GM
authors.lastname: Reynolds
authors.forename: A
authors.initials: A
authors.lastname: Cruchley
authors.forename: D M
authors.initials: DM
authors.lastname: Williams
authors.forename: N
authors.initials: N
authors.lastname: Müller-Lantzsch
authors.forename: L S
authors.initials: LS
authors.lastname: Young
mesh_terms: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis*; D019309:Epstein-Barr Virus Nuclear Antigens; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D017733:Leukoplakia, Hairy / Q000821:virology*; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D013274:Stomach Neoplasms / Q000821:virology*; D014412:Tumor Virus Infections / Q000150:complications
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens