Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP.
We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.
Moriyoshi
K
K
Institute for Immunology Kyoto University Faculty of Medicine Kyoto.
Richards
L J
LJ
Akazawa
C
C
O'Leary
D D
DD
Nakanishi
S
S
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D066298:In Vitro Techniques; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D037401:Scyphozoa / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000502:physiology
D008164:Luminescent Proteins; D015335:Molecular Probes; D014760:Viral Fusion Proteins; D049452:Green Fluorescent Proteins