title: Graft-versus-host disease in limb transplantation: digital image analysis of bone marrow and TGF-beta expression in situ using a novel 3-D microscope.
abstract: A subpopulation of parental to hybrid VBMT recipients developed characteristic clinical and histopathologic manifestations of GVHD. These changes are similar to those seen in human GVHD secondary to bone marrow transplantation. Human GVHD also manifests itself in an acute and chronic manner. Only a minority (30% to 40%) of animals developed lethal GVHD in our model. Those animals developing GVHD had a significantly (P < .0001) higher expression of TGF-beta in situ compared to the tolerant subpopulation. The differential expression of TGF-beta may represent an important mechanism of immune dysregulation associated with GVHD in CTA recipients.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, USA.
authors.forename: R
authors.initials: R
authors.lastname: Ramsamooj
authors.forename: R
authors.initials: R
authors.lastname: Llull
authors.forename: L D
authors.initials: LD
authors.lastname: Tatem
authors.forename: K S
authors.initials: KS
authors.lastname: Black
authors.forename: V
authors.initials: V
authors.lastname: Lotano
authors.forename: R M
authors.initials: RM
authors.lastname: Dalsey
authors.forename: C T
authors.initials: CT
authors.lastname: Born
authors.forename: W G
authors.initials: WG
authors.lastname: DeLong
authors.forename: C W
authors.initials: CW
authors.lastname: Hewitt
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000098:blood supply* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000473:pathology*; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000473:pathology*; D006614:Hindlimb / Q000637:transplantation*; D006801:Humans; D008853:Microscopy / Q000379:methods; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000096:biosynthesis; D014184:Transplantation, Homologous / Q000473:pathology*
chemical_list: D016212:Transforming Growth Factor beta