title: [Cloning and primary structure determination of genes coding for IE2 and PP150 proteins of human cytomegalovirus].
abstract: The epidemiological situation with cytomegalovirus infection in Siberia is still to be studied and serological diagnosis of human cytomegalovirus (HCMV) is not satisfactory. Two regions of HCMV genome (strain AD-169) have been examined for obtaining diagnostic reagents by expression cloning. Using polymerase chain reaction, fragments of gene of the immediate early protein (IE2) and of the region encoding for the entire hydrophilic part (1176 bp) of the large phospoprotein gene (pp150) have been obtained. Both fragments were cloned in bacterial vectors. Analysis of nucleotide sequence showed negligible substitutions in comparison with previously reported sequences for these genes.
authors.forename: M A
authors.initials: MA
authors.lastname: Susloparov
authors.forename: P A
authors.initials: PA
authors.lastname: Belavin
authors.forename: A V
authors.initials: AV
authors.lastname: Krendel'shchikov
authors.forename: A I
authors.initials: AI
authors.lastname: Bedristov
authors.forename: M M
authors.initials: MM
authors.lastname: Bakhtina
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: A A
authors.initials: AA
authors.lastname: Chepurnov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D004274:DNA, Recombinant; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus; C056367:pp150 protein, Cytomegalovirus
vernacular_title: Klonirovanie i opredelenie pervichnoĭ struktury genov belkov IE2 i PP150 tsitomegalovirusa cheloveka.