[Atheroma plaque and Apo E alleles].
The authors report their experience regarding the identification of Apo-E alleles on atheroma carotid plaques in 20 patients of both sexes diagnosed as suffering from severe carotid stenosis using Doppler tests. A DNA hybridization and amplification method was used to identify Apo E-2, Apo E-3 and Apo E-4 alleles and their various phenotypical combinations. The following results were obtained in the 20 plaques examined: Apo E-3/E-4 in 114 patients (70%), 2 diabetic patients Apo E-4/E-3, one vascular demented patient Apo E-2/E-3, and 3 plaques defined as severely calcified Apo E-2/E-2. It can therefore be seen that the majority of plaques (70%), considered a risk for future stroke due to altered carotid Doppler tests, does not differ greatly by the homozygote allele Apo E-3/E-3 commonly found in the blood of the so-called "normal" population. It is difficult to draw any conclusions from the alleles found in the other 5 patients due to their scarce statistical value and the limited number of carotid plaques examined, but there appears to be some sort of correlation between calcified plaque, hyperlipidemia and the allele Apo E-2/E-2, with an interchange of position between cysteine arginine amino acids in the Apo E sequences.
Venarucci
D
D
INRCA Istituto a Carattere Scientifico-Fermo.
Venarucci
V
V
Casado
A
A
De La Torre
R
R
D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics*; D001161:Arteriosclerosis / Q000000981:diagnostic imaging / Q000235:genetics*; D016893:Carotid Stenosis / Q000000981:diagnostic imaging / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005260:Female; D006720:Homozygote; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D014463:Ultrasonography
D001057:Apolipoproteins E; D004247:DNA
Placca ateromasica ed alleli delle Apo E.