Development of monoclonal antibodies for the selective isolation of human plasma apolipoprotein A-containing particles.
Monoclonal antibodies (MAbs) to human plasma apolipoprotein A-I (apo A-I), denoted FF9 and 6B9, and apolipoprotein A-II (apo A-II), 3F5, were developed to be used in an immunoaffinity chromatography procedure to isolate lipoprotein particles Lp A-I and Lp A-I:A-II. MAb FF9 and MAb 6B9 reacted with apo A-I and high-density lipoprotein (HDL) while MAb 3F5 was directed to apo A-II and HDL. The apparent affinity constant (Kapp) for apo A-I of the MAb FF9 was higher (2 x 10(7) M-1) than that of 6B9 (5 x 10(6) M-1). MAb 3F5 recognized the apo A-II with a Kapp value of 1 x 10(9) M-1. The isolated lipoparticles Lp A-I and Lp A-I:A-II will be used to standardize an immunoassay for the measurement of these apo A-I-containing lipoprotein particles in human plasma.
Calvo
C
C
Departamento Bioquímica Clínica e Immunologiía, Facultad de Farmacia, Universidad de Concepción, Chile.
Bustos
P
P
Sepulveda
J
J
Ulloa
N
N
Sepulveda
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000915:Antibody Affinity; D000918:Antibody Specificity; D016632:Apolipoprotein A-I / Q000097:blood* / Q000276:immunology* / Q000302:isolation & purification; D016633:Apolipoprotein A-II / Q000097:blood* / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II