A novel neuro-muscular marker for the heavy subunit of neurofilament proteins, NF-H, and striated muscle myosin of Xenopus laevis.
A novel monoclonal antibody, M7, is described, that reacts on Western blots with the large subunit of the neurofilament triplet proteins (NF-H) and with striated muscle myosin of Xenopus laevis. Enzymatically digested neurofilament and myosin proteins revealed different immunoreactive peptide fragments on Western blots. Therefore, the antibody must react with immunologically related epitopes common to both proteins. Immunohistochemistry showed staining of large and small axons in CNS and PNS, and nerves could be followed into endplate regions of skeletal muscles. These muscles were characterized by a striated immunostaining of the M-lines. Despite the crossreactivity of M7 with NF-H and muscle myosin, this antibody may be a tool to study innervation of muscle fibers, and to define changes in the neuromuscular organization during early development and metamorphosis of tadpoles.
Riederer
B M
BM
Institut d'Anatomie, Lausanne, Switzerland.
Marugg
R A
RA
Schönenberger
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers; D002918:Chymotrypsin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology; D009218:Myosins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D016900:Neurofilament Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D012697:Serine Endopeptidases / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016900:Neurofilament Proteins; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase; D014357:Trypsin; D009218:Myosins