title: Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins.
abstract: The potential role of a molecular chaperone on the rate of production of extensively altered Bacillus thuringiensis Cry1C proteins was investigated. Analysis of the proteins produced by the recombinant B. thuringiensis strains showed that the truncated proteins were produced at a low rate. Expression of the 20-kDa protein gene from B. thuringiensis ssp. israelensis in tandem with the truncated-cry1C genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20-kDa protein gene was expressed.
authors.affiliation: BIOTROP-IGEPAM, CIRAD, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Rang
authors.forename: M
authors.initials: M
authors.lastname: Bes
authors.forename: V
authors.initials: V
authors.lastname: Lullien-Pellerin
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: B A
authors.initials: BA
authors.lastname: Federici
authors.forename: R
authors.initials: R
authors.lastname: Frutos
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004731:Endotoxins / Q000096:biosynthesis* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins; D018832:Molecular Chaperones / Q000502:physiology*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D015183:Restriction Mapping
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis