Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer.
Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybrydization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T-cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.
Shpitz
B
B
Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada.
Stern
H
H
Sandhu
J S
JS
Reilley
R M
RM
Tropak
M B
MB
Jansz
G
G
Xu
J
J
Gallinger
S
S
D000818:Animals; D018033:Antibodies, Bispecific / Q000633:toxicity*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002469:Cell Separation; D003110:Colonic Neoplasms / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000145:classification; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000502:physiology
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes