A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes.
Oligonucleotide primers were designed and successfully applied to amplify DNA fragments of dNDP-glucose dehydratase genes from actinomycete species producing natural compounds which contain deoxysugar moieties. The deduced amino acid sequence of the isolated fragments revealed similarity to known dNDP-glucose dehydratases. A phylogeny for the deduced proteins of the obtained fragments and for dNDP-glucose dehydratases described in the data bases was constructed. dNDP-glucose dehydratases from actinomycetes were more related to each other than to dehydratases from species of other orders. The phylogenetic analysis also revealed a close relation between dehydratases from strains producing natural compounds with similar deoxysugar moieties.
Decker
H
H
Eberhard-Karls-Universität Tübingen, Mikrobiologie, Germany.
Gaisser
S
S
Pelzer
S
S
Schneider
P
P
Westrich
L
L
Wohlleben
W
W
Bechthold
A
A
D000190:Actinomyces / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005820:Genetic Testing; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics
D006836:Hydro-Lyases