title: Rapid detection of chemical carcinogens by measuring RNA released from microsomes in post-mitochondrial supernatant.
abstract: Incubation of carcinogens with post-mitochondrial supernatant (PMS) and NADPH releases ribosomes from microsomes resulting in increased RNA concentration in post-microsomal supernatant. However, non-carcinogens fail to do so. Enhanced concentration of RNA in test over control samples can provide a useful index for the carcinogenicity of environmental pollutants.
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh, India.
authors.forename: J S
authors.initials: JS
authors.lastname: Dhanda
authors.forename: H M
authors.initials: HM
authors.lastname: Dani
authors.forename: R
authors.initials: R
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D012313:RNA / Q000032:analysis*; D051381:Rats; D013997:Time Factors
chemical_list: D002273:Carcinogens; D012313:RNA