Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2.
Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.
Sunden
S L
SL
Department of Pediatrics, University of Iowa, Iowa City 52242, USA.
Businga
T
T
Beck
J
J
McClain
A
A
Gastier
J M
JM
Pulido
J C
JC
Yandava
C N
CN
Brody
T
T
Ghazizadeh
J
J
Weber
J L
JL
Duyk
G M
GM
Murray
J C
JC
Buetow
K H
KH
Sheffield
V C
VC
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics; D006224:Cricetinae; D005260:Female; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats; D018911:Trinucleotide Repeats
D005819:Genetic Markers