Glycosylation of alpha 1-acid glycoprotein in septic shock: changes in degree of branching and in expression of sialyl Lewis(x) groups.
The occurrence of differences in acute-phase response, with respect to concentration and glycosylation of alpha 1-acid glycoprotein (AGP) was studied in the sera of patients, surviving or not from septic shock. Crossed affino-immunoelectrophoresis was used with concanavalin A and Aleuria aurantia lectin for the detection of the degree of branching and fucosylation, respectively, and the monoclonal CSLEX-1 for the detection of sialyl Lewisx (SLeX) groups on AGP. Septic shock apparently induced an acute-phase response as indicated by the increased serum levels and changed glycosylation of AGP. In the survivor group a transient increase in diantennary glycan content was accompanied by a gradually increasing fucosylation and SLeX expression, comparable to those observed in the early phase of an acute-inflammatory response. Remarkably, in the non-survivor group a modest increase in diantennary glycan content was accompanied by a strong elevation of the fucosylation of AGP and the expression of SLeX groups on AGP, typical for the late phase of an acute-phase response. Our results suggest that these changes in glycosylation of AGP can have a prognostic value for the outcome of septic shock.
Brinkman-van der Linden
E C
EC
Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
van Ommen
E C
EC
van Dijk
W
W
D000210:Acute-Phase Reaction; D000328:Adult; D000368:Aged; D002240:Carbohydrate Sequence; D003208:Concanavalin A; D005260:Female; D006031:Glycosylation; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D037102:Lectins; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D009961:Orosomucoid / Q000737:chemistry* / Q000302:isolation & purification; D011379:Prognosis; D012772:Shock, Septic / Q000097:blood*; D000080506:Sialyl Lewis X Antigen
D037102:Lectins; D009844:Oligosaccharides; D009961:Orosomucoid; D000080506:Sialyl Lewis X Antigen; C064549:lectin, Aleuria aurantia; D003208:Concanavalin A