Inhaled nitric oxide injures the pulmonary surfactant system of lambs in vivo.
Nitric oxide (.NO) is a free radical, and as such may damage the pulmonary surfactant system. To determine the potential toxicity of .NO in vivo, we exposed 35 newborn lambs to 0, 20, 80 or 200 ppm .NO in either 21 or 60% O2 for 6 h. At the end of the exposure, lambs had normal values of arterial Po2, Pco2, and pH; total protein concentration in the bronchoalveolar lavage was also at normal levels. There were no differences in the surface properties of surfactant among the air or 60% O2 groups. Pulmonary surfactant samples, isolated from the bronchoalveolar lavage of lambs breathing air or 20 ppm .NO and reconstituted at a lipid concentration of 3 mg/ml, reached a low minimum surface tension (Tmin < 3 mN/m) in a pulsating bubble surfactometer. On the other hand, abnormal surface properties were observed in 36 and 60% of surfactant samples isolated from lungs of lambs that breathed 80 or 200 ppm .NO, respectively. These findings were confirmed using a captive bubble surfactometer. Surfactant protein A, isolated from the lungs of lambs that breathed 200 ppm .NO, exhibited decreased ability to aggregate lipids in vitro. These data are consistent with injury to the surfactant apoproteins during inhalation of either 80 or 200 ppm .NO for 6 h.
Matalon
S
S
Department of Pediatrics, University of Alabama at Birmingham 35233, USA. smatalon@ms.tht.anes.uab.edu
DeMarco
V
V
Haddad
I Y
IY
Myles
C
C
Skimming
J W
JW
Schürch
S
S
Cheng
S
S
Cassin
S
S
D000280:Administration, Inhalation; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000494:pharmacology; D009994:Osmolar Concentration; D011510:Proteolipids / Q000737:chemistry / Q000502:physiology; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D012756:Sheep; D013329:Structure-Activity Relationship; D013500:Surface Tension
D011510:Proteolipids; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D009569:Nitric Oxide