title: Nucleotide sequences of alcohol acetyltransferase genes from lager brewing yeast, Saccharomyces carlsbergensis.
abstract: The nucleotide sequences of alcohol acetyltransferase genes isolated from lager brewing yeast, Saccharomyces carlsbergensis have been determined. S. carlsbergensis has one ATF1 gene and another homologous gene, the Lg-ATF1 gene. There was a high degree of homology between the amino acid sequences deduced for the ATF1 protein and the Lg-ATF1 protein (75.7%), but the N-terminal region has a relatively low degree of homology. Southern analysis and contour-clamped homogeneous electric field analysis of Saccharomyces strains suggest that the ATF1 gene is located on chromosome XV in S. cerevisiae and that the Lg-ATF1 gene might originate from the "non-S. cerevisiae' genome of S. carlsbergensis, which is similar to that of S. bayanus and S. pastorianus.
authors.affiliation: Central Laboratories for Key Technology, Kirin Brewery Co. Ltd, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Yoshimoto
authors.forename: N
authors.initials: N
authors.lastname: Nagasawa
authors.forename: T
authors.initials: T
authors.lastname: Bogaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
authors.forename: M
authors.initials: M
authors.lastname: Hamachi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D011506:Proteins; D012440:Saccharomyces / Q000201:enzymology / Q000235:genetics*; D017385:Sequence Homology
chemical_list: D011506:Proteins; D000123:Acetyltransferases; C079238:alcohol O-acetyltransferase