title: Protein phosphatase mRNA expression in Purkinje cells of staggerer and reeler mutant mice.
abstract: We used in situ hybridization to search for the expression of three protein phosphatase (PP) mRNAs in Purkinje cells of normal mice, and staggerer (sg/sg) and reeler (rl/rl) mutant mice, two strains with known Purkinje cell disorders. The expression of the mRNAs was comparable in the normal and rl/rl Purkinje cells, but considerably reduced in the sg/sg. We interpret this finding as indicating: (a) the staggerer mutant gene may directly affect the phosphatase component of the protein phosphorylation-dephosphorylation cycle in the sg/sg Purkinje cells; or (b) the reduced mRNA expression may be a secondary phenomenon, resulting from abnormal Purkinje cell function due to the lack of synaptic input.
authors.affiliation: Department of Morphology, Tokai University School of Medicine, Isehara, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: O
authors.initials: O
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Shima
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: H
authors.initials: H
authors.lastname: Abe
mesh_terms: D000818:Animals; D017403:In Situ Hybridization; D051379:Mice; D008818:Mice, Neurologic Mutants; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011689:Purkinje Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012016:Reference Values
chemical_list: D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases