Effect of glucose on photodynamic action of methylene blue in Escherichia coli cells.
The results of this work show that the resistance of Escherichia coli cells to the photodynamic action of methylene blue is increased by the addition of glucose to the media in which they are grown. It is postulated that the increased resistance may be due to lowered retention of the dye by cells grown in the presence of glucose, leading to the diminution in DNA damage revealed in the alkaline sucrose gradients. The role of cyclic adenosine-monophosphate in the protective action of glucose is discussed.
Capella
M
M
Instituto de Biofisica Carlos Chagas Filho, Centro de Clênclas da Saúde, Rio de Janeiro, Brazil.
Coelho
A M
AM
Menezes
S
S
D000242:Cyclic AMP / Q000378:metabolism; D004249:DNA Damage; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005947:Glucose / Q000494:pharmacology*; D008751:Methylene Blue / Q000493:pharmacokinetics / Q000494:pharmacology*; D018462:Photobiology; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*
D017319:Photosensitizing Agents; D000242:Cyclic AMP; D005947:Glucose; D008751:Methylene Blue