Hypoglycemia prevents increase in lactic acidosis during reperfusion after temporary cerebral ischemia in rats.
Sequential 31P and 1H MRS was used to measure cerebral phosphate metabolites, intracellular pH, and lactate in normoglycemic and hypoglycemic rats during 30 min of complete cerebral ischemia and 5.5 h of reperfusion. These results were correlated with brain levels of free fatty acids (FFAs), excitatory amino acids, cations, and water content at death. The lactate/N-acetyl aspartate ratio was not significantly different between groups before or during occlusion. During reperfusion, the ratio was higher in normoglycemic rats from 3 to 85 min (p < or = 0.05), and recovery time was faster in hypoglycemic rats (29 vs 45 min; p = 0.04), suggesting reduced lactate production and faster recovery of aerobic metabolism. During occlusion, significant but comparable decrease of intracellular pH occurred in each group. Intracellular pH was higher in hypoglycemic rats at 140 min and 260 min of reperfusion. Water content, Na and K+ concentrations, and FFA and excitatory amino acid levels were not significantly different between groups, but hypoglycemic rats had less depletion of levels of Mg2+ (p = 0.011). These results show that hypoglycemia has a limited but potentially beneficial effect on postischemic lactic acidosis.
Sappey-Marinier
D
D
Magnetic Resonance Unit, Veterans Administration Medical Center, San Francisco, CA 94121, USA.
Chileuitt
L
L
Weiner
M W
MW
Faden
A I
AI
Weinstein
P R
PR
D000140:Acidosis, Lactic / Q000097:blood / Q000517:prevention & control*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002545:Brain Ischemia / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D007003:Hypoglycemia / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D007773:Lactates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
D001786:Blood Glucose; D007773:Lactates; D001224:Aspartic Acid; C000179:N-acetylaspartate