Detection of Genomic Polymorphism in Plasmodium falciparum using an arbitrarily primed PCR assay.
Modifications of the arbitrarily primed polymerase chain reaction assay (i.e. a low annealing temperature and a very slow increase in the temperature during the elongation steps during the amplification cycles) allowed it to be used with the AT-rich Plasmodium falciparum DNA. The analysis of the products by polyacrylamide-urea gels, after silver staining, resulted in high resolution and sensitivity. Eighteen single and six combined pairs of arbitrary primers were tested. Two produced polymorphic patterns complex enough to differentiate between close Colombian isolates in a single assay. This method may be useful in studying the distribution and migration of strains in endemic areas, and for identifying intralaboratory cross-contamination of cultures.
Rojas
M O
MO
Faculty of Sciences Universidad Nacional, Instituto Nacional de Salud, Bogotá, Colombia.
De-Castro
J
J
Mariño
G
G
Wasserman
M
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
D017931:DNA Primers