Human dermal fibroblasts express eotaxin: molecular cloning, mRNA expression, and identification of eotaxin sequence variants.
Recently we discovered and purified a novel beta-chemokine with eosinophil specific chemotactic activity from supernatants to long-term TNF-alpha stimulated dermal fibroblasts. Using degenerated specific oligonucleotides based on partial amino acid sequence data and a PCR protocol, we obtained different clones sharing high sequence homology with this novel chemokine and with human eotaxin cDNA. Semi-quantitative RT-PCR experiments using eotaxin-specific primers indicate low constitutive eotaxin mRNA expression in human dermal fibroblasts which is upregulated by IL-1 alpha and TNF-alpha within 6 hrs and modulated by IFN-gamma. While IL-1 alpha-induced eotaxin mRNA accumulation is transient, long-term stimulation with TNF-alpha resulted in a further increase of eotaxin mRNA.
Bartels
J
J
Department of Dermatology, University of Kiel, Germany.
Schlüter
C
C
Richter
E
E
Noso
N
N
Kulke
R
R
Christophers
E
E
Schröder
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D054413:Chemokine CCL11; D019742:Chemokines, CC; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D018076:DNA, Complementary; D012333:RNA, Messenger