Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases.
We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.
Heinz
B A
BA
Infectious Disease Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.
Tang
J
J
Labus
J M
JM
Chadwell
F W
FW
Kaldor
S W
SW
Hammond
M
M
D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012229:Rhinovirus / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D014764:Viral Proteins
D011480:Protease Inhibitors; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases