The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges.
The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.
van den Hooven
H W
HW
Department of Biophysical Chemistry, NIZO (Netherlands Institute for Dairy Research) Ede, The Netherlands.
Lagerwerf
F M
FM
Heerma
W
W
Haverkamp
J
J
Piard
J C
JC
Hilbers
C W
CW
Siezen
R J
RJ
Kuipers
O P
OP
Rollema
H S
HS
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D001430:Bacteriocins / Q000737:chemistry; D013294:Lactococcus lactis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides; D013440:Sulfides / Q000737:chemistry
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D010455:Peptides; D013440:Sulfides; C099477:variacin; C082508:lacticin 481; C082674:salivaricin A; C016887:A-FF22