Cloning of a cDNA encoding the small subunit of cytochrome b558 (cybS) of mitochondrial fumarate reductase (complex II) from adult Ascaris suum.
Complex II in the mitochondria of the adult parasitic nematode, Ascaris suum, exhibits high fumarate reductase activity in addition to succinate dehydrogenase activity and plays a key role in the anaerobic energy metabolism of the worm. In this study, the amino acid sequence of the small subunit of cytochrome b558 (cybS) in adult complex II was deduced from the cDNA isolated by immunoscreening an A. suum muscle cDNA library. Histidine residues, which are possible heme axial ligands in cytochrome b558, were found in the second transmembrane segment of the subunit. This is the first report of the primary structure of the small subunit in the two-subunit cytochrome b in mitochondrial complex II from a multicellular eukaryote.
Saruta
F
F
Department of Parasitology, University of Tokyo, Japan.
Hirawake
H
H
Takamiya
S
S
Ma
Y C
YC
Aoki
T
T
Sekimizu
K
K
Kojima
S
S
Kita
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001200:Ascaris / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology; D008969:Molecular Sequence Data; D019255:NADPH Oxidases; D017386:Sequence Homology, Amino Acid; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000235:genetics*
D003573:Cytochrome b Group; D018076:DNA, Complementary; D018509:DNA, Helminth; C036888:cytochrome b558; D013385:Succinate Dehydrogenase; D019255:NADPH Oxidases