Localization of reactive tyrosine residues of baker's yeast transketolase.
Baker's yeast transketolase inactivated by tetranitromethane was digested with Staphylococcus aureus V8 protease. Four peptides absorbing at 360 nm were isolated by reverse-phase HPLC and sequenced. The modified tyrosines were identified as Tyr-184, Tyr-210 and Tyr-370.
Kovina
M
M
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
Viryasov
M
M
Baratova
L
L
Kochetov
G
G
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism; D014174:Transketolase / Q000737:chemistry* / Q000378:metabolism*; D014443:Tyrosine / Q000737:chemistry* / Q000378:metabolism
D010446:Peptide Fragments; D014443:Tyrosine; D014174:Transketolase; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase