Gene identification by 3' terminal exon trapping.
3' terminal exon trapping offers a powerful and efficient technology for rendering fragments of transcribed genes from large stretches of unsequenced, vertebrate, genomic DNA. The products from this methodology are the starting point for further studies of gene discovery and analysis. Future considerations for this technology include answering questions about the degree of efficiency from various trapping substrates and whether or not this approach can be scaled up for large-scale gene discovery such as individual chromosome or whole genomic approaches. With the onset of EST database searching, the final analysis becomes a computer assay that is consistent with the future of genetics as this field continues to move towards informational experimentation based on DNA sequence.
Krizman
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National Institutes of Health, National Center for Human Genome Research, Bethesda, MD 20892, USA.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D005091:Exons; D005796:Genes; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D014714:Vertebrates