title: Phosphatidic acid activation of protein kinase C in LA-N-1 neuroblastoma cells.
abstract: Phosphatidic acid (PA), a hydrolytic product of phospholipase D activity, stimulated cytosolic protein kinase C (PKC) activity when LA-N-1 neuroblastoma cells in culture were treated with PA, without translocating the enzyme to the membrane. Treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) translocated and activated PKC in a dogmatic manner. Partially purified PKC activity derived from LA-N-1 neuroblastoma cells was stimulated by PA alone or in the presence of phosphatidylserine or TPA, without affecting [3H]phorbol dibutyrate binding, indicating that the site of action of PA was different from the phorbol ester or diacylglycerol binding site. These results suggest an unorthodox pattern of PKC stimulation mediated by PA which appears to be yet another mode of PA signal transduction.
authors.affiliation: Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Centre de Neurochimie du C.N.R.S., Strasbourg, France.
authors.forename: D
authors.initials: D
authors.lastname: Lang
authors.forename: A N
authors.initials: AN
authors.lastname: Malviya
authors.forename: A
authors.initials: A
authors.lastname: Hubsch
authors.forename: J N
authors.initials: JN
authors.lastname: Kanfer
authors.forename: L
authors.initials: L
authors.lastname: Freysz
mesh_terms: D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000494:pharmacology*; D011493:Protein Kinase C / Q000187:drug effects*; D013997:Time Factors
chemical_list: D010712:Phosphatidic Acids; D011493:Protein Kinase C; D002118:Calcium