title: Oligosaccharides derived from the xyloglucan isolated from the seeds of Hymenaea courbaril var. stilbocarpa.
abstract: On aqueous extraction, Hymenaea courbaril var. stilbocarpa, known in Brazil as jatobá, furnishes a high yield of viscous xyloglucan (45%) from its seeds. The crude polysaccharide (B1) was hydrolysed and the products, analysed as alditol acetates, were glucose, xylose, galactose and arabinose in the ratio 50:35:13:2. After further fractionation on DEAE-cellulose column (chloride form), the main fraction (70% yield, B2) was obtained. The basic structure of the xyloglucan was determined as a cellulose-type (1-->4)-linked beta-D-glucan backbone partially substituted with side chains at O6 of alpha-D-xylopyranose, some of which were themselves substituted at O2 by the units of beta-D-galactopyranose. Treatment of the xyloglucan (B2) with commercial cellulase from Trichoderma sp. yielded six oligosaccharides. These oligosaccharides were isolated by preparative paper chromatography, and their structures were determined by gas-liquid chromatography-mass spectroscopy of the derived partially O-methylated alditol acetates. These results confirm the structure proposed for jatobá seed xyloglucan.
authors.affiliation: Department of Chemistry, Pontificia Universidade Católica, Curitiba, Paraná, Brazil.
authors.forename: N N
authors.initials: NN
authors.lastname: Lima
authors.forename: C G
authors.initials: CG
authors.lastname: Rechia
authors.forename: J L
authors.initials: JL
authors.lastname: Ganter
authors.forename: F
authors.initials: F
authors.lastname: Reicher
authors.forename: M R
authors.initials: MR
authors.lastname: Sierakowski
mesh_terms: D001938:Brazil; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism; D002854:Chromatography, Paper; D007887:Fabaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012995:Solubility; D014867:Water; D014990:Xylans
chemical_list: D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; D014867:Water; C029353:xyloglucan; D002480:Cellulase