Isolation and characterization of a bradykinin potentiating peptide (BPP-S) isolated from Scaptocosa raptoria venom.
Venom of the spider Scaptocosa raptoria was fractionated by chromatography on Sephadex G-10 followed by HPLC, and a bradykinin potentiating peptide, BPP-S, was obtained in pure form. The amino acid sequence of this undecapeptide is presented. Peptide BPP-S significantly potentiates the effects of bradykinin on smooth muscle, and inhibits the angiotensin-converting enzyme (ACE) in vitro.
Ferreira
L A
LA
Departamento de Bioquimica e Biofisica, Instituto Butantan, São Paulo, Brazil.
Alves
W E
WE
Lucas
M S
MS
Habermehl
G G
GG
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D017837:Bothrops; D001920:Bradykinin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D003435:Crotalid Venoms; D009842:Oligopeptides; C006791:bradykinin potentiating factors; D001920:Bradykinin