title: Specific binding component of the "inactive" stereoisomer (S,S)-[125I] IQNB to rat brain muscarinic receptors in vivo.
abstract: In vivo nonspecific binding can be estimated using the inactive stereoisomer of a receptor radioligand. However, the binding of the inactive stereoisomer may be partially specific. Specific binding of the inactive (S,S)-[125I]IQNB was estimated from the inhibition induced by a competing nonradioactive ligand. This technique differed from the usual approach, since it was used to study the inactive rather than the active stereoisomer. The results indicate that there is substantial specific binding for (S,S)-[125I]IQNB.
authors.affiliation: Department of Radiology, George Washington University Medical Center, Washington DC 20037, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Boulay
authors.forename: R C
authors.initials: RC
authors.lastname: McRee
authors.forename: V I
authors.initials: VI
authors.lastname: Cohen
authors.forename: V K
authors.initials: VK
authors.lastname: Sood
authors.forename: B R
authors.initials: BR
authors.lastname: Zeeberg
authors.forename: R C
authors.initials: RC
authors.lastname: Reba
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D011336:Probability; D011813:Quinuclidinyl Benzilate / Q000378:metabolism* / Q000493:pharmacokinetics; D011869:Radioligand Assay / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000378:metabolism; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
chemical_list: D007457:Iodine Radioisotopes; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate