The effects of the somatostatin analog octreotide on angiogenesis in vitro.
This study examined the in vitro antiangiogenic effects of the somatostatin analog octreotide on the growth of human HUV-EC-C endothelial cells and vascular cells from explants of rat aorta cultured on fibronectin-coated dishes or included in fibrin gel. A total 10(-9) mol/L octreotide reduced the mean uptake of 3H-thymidine by HUV-EC-C cells by 37% compared with controls. The 10(-8) mol/L concentration of octreotide inhibited the proliferation of endothelial and smooth muscle cells growing on fibronectin by 32.6% and reduced the sprouting of cells from the adventitia of aortic rings in fibrin by 33.2% compared with controls, as measured by tetrazolium bioreduction and image analysis, respectively. These results demonstrate that octreotide is an effective inhibitor of vascular cell proliferation in vitro.
Danesi
R
R
Scuola Superiore di Studi Universitari e di Perfezionamento S. Anna, Pisa, Italy.
Del Tacca
M
M
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D015282:Octreotide / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
D015282:Octreotide