Morphometric analysis of the small intestinal epithelium in the indomethacin-treated mouse.
To obtain a clearer understanding of the changes which are induced in the small intestine of the mouse by an ulcerogenic dose of indomethacin, a quantitative analysis of the nonulcerated small intestinal mucosa was performed in mice that were given 2 injections of indomethacin at a dose of 85 mg/kg body weight. At 20 h after the administration of the drug, values were obtained for epithelial volume, whole crypt number, and for the number of profiles of columnar, Paneth, entero-endocrine and goblet cells and cryptal mitotic figures in the small intestine. Comparison of the values obtained from indomethacin-treated mice with those from control mice showed that there were fewer whole crypts and a reduced epithelial volume in the jejunum and ileum in indomethacin-treated mice. The numbers of columnar and Paneth cell profiles and of mitotic figures were significantly greater in the jejunal and ileal crypts in indomethacin-treated mice than in controls. These findings suggest that the administration of high-dose indomethacin in the mouse leads to crypt losses and increased mitotic activity in the nonulcerated parts of the small intestine.
Ettarh
R R
RR
Department of Anatomy, University College Dublin, Ireland, UK.
Carr
K E
KE
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008940:Mitotic Index
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007213:Indomethacin