Expression of platelet-derived growth factor beta receptor in chondrogenesis of perichondrial transplants.
An experimental study was done in two rabbits to establish whether the early stages of cartilage generation involves stimulation via the platelet-derived growth factor beta receptor (PDGFR-beta). Rib perichondrium was dissected off and transplanted to the knee joint. The perichondrial grafts were removed for microscopic investigation at day 0 and day 6 and kept frozen until analysed by immunohistochemistry and in situ hybridisation for the presence of PDGF beta receptor protein and mRNA, respectively. Samples from day 0 showed sparse cells with a positive immunohistochemical reaction. In samples from day 6 there were signs of perichondrial proliferation and chondroid maturation and a more widespread immunohistochemical staining reaction could be seen mainly on proliferating perichondrial cells, but also on some chondrocytes. In situ hybridisation confirmed the expression of PDGF beta receptor mRNA in the same areas of serial sections. These findings may indicate that PDGF beta receptor activity is part of the early response after autotransplantation of perichondrium.
Ljung
A
A
Department of Plastic and Hand Surgery, University Hospital, Uppsala, Sweden.
Skoog
V
V
Widenfalk
B
B
Ren
Z
Z
Nistér
M
M
Ohlsén
L
L
D000818:Animals; D002356:Cartilage / Q000473:pathology / Q000502:physiology* / Q000637:transplantation*; D006085:Graft Survival; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007717:Knee; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D012272:Ribs
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger