Cloning and expression of cDNA for O-acetylation of GD3 ganglioside.
O-acetylated sialic acids of glycoproteins and gangliosides show cell type-specific and developmentally regulated expression in various systems and sometimes reappear as oncofetal antigens. O-acetylation of sialic acids may influence cell-cell interactions mediated by the sialic acid-binding lectins. Here we describe the molecular cloning and sequencing of a gene that produces O-acetyl disialoganglioside (O-acetyl GD3). Expression analysis showed that this gene product participates the O-acetylation of GD3 ganglioside sialic acid. The deduced amino acid sequence of the product was found to be similar to the milk fat globule membrane glycoprotein.
Ogura
K
K
Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.
Nara
K
K
Watanabe
Y
Y
Kohno
K
K
Tai
T
T
Sanai
Y
Y
D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D005732:Gangliosides / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D005732:Gangliosides; D018396:Mucin-1; C026226:ganglioside, GD3; C049132:9-O-acetyl-GD3 ganglioside; D000123:Acetyltransferases; C075983:ganglioside O-acetyltransferase