Activation of p42erk2 MAPK and p90rsk by IL-2 occurs independently of protein kinase C.
Stimulation of the human interleukin-2 (IL-2)-dependent cell line, Kit225, with IL-2 resulted in the rapid activation of microtubule-associated protein-2 kinase (MAPK), as demonstrated by the tyrosine phosphorylation of p42erk2, the reduced mobility of MAPK in SDS-PAGE gels, and the increased ability of lysates from these cells to phosphorylate myelin basic protein. Measurements of kinase activity in immunoprecipitates of p9O ribosomal S6 kinase (p90rsk) demonstrated that stimulation of this cell line with IL-2 also resulted in the activation of p90rsk. Further, increased MAPK activity occurred following IL-2 stimulation, but not following phorbol 12-myristate 13-acetate (PMA) stimulation in cells depleted of PKC by prolonged treatment with a high concentration of PMA. These results demonstrate that IL-2 stimulation results in the activation of MAPK and p90rsk and that this activation occurs in a PKC-independent manner.
Franklin
R A
RA
Division of Basic Sciences, National Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
Sawami
H
H
Terada
N
N
Lucas
J J
JJ
Gelfand
E W
EW
D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D048370:MAP Kinase Kinase 2; D019950:Mitogen-Activated Protein Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D019893:Ribosomal Protein S6 Kinases; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology
D007376:Interleukin-2; C482128:MAP2K2 protein, human; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D019893:Ribosomal Protein S6 Kinases; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048370:MAP Kinase Kinase 2; D020929:Mitogen-Activated Protein Kinase Kinases