Mapping of the porcine urate oxidase and transforming growth factor beta 2 genes by fluorescence in situ hybridization.
We have mapped two genes from human chromosome 1, urate oxidase (UOX) and transforming growth factor beta 2 (TGFB2), by fluorescence in situ hybridization (FISH) in the pig genome. Porcine-specific polymerase chain reaction (PCR) primers for both genes were designed from the porcine cDNA sequence. With the help of these primers yeast artificial chromosome (YAC) clones for UOX and TGFB2 were isolated from a pig YAC library. These DNA probes were used for FISH analysis. TGFB2 was localized to SSC 10p16. With the YAC probe for UOX two porcine chromosome regions 6q26 and 6q32, revealed specific signals. These results, help to refine the comparative mapping data between human and pig.
Rettenberger
G
G
Institut für Nutztierwissenschaften, Eidgenössische Technische Hochschule ETH-Z, Zürich, Switzerland.
Leeb
T
T
Meier-Ewert
S
S
Bruch
J
J
Klett
C
C
Brenig
B
B
Hameister
H
H
D000818:Animals; D001483:Base Sequence; D001710:Biotin; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D017931:DNA Primers; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014503:Urate Oxidase / Q000235:genetics*
D017931:DNA Primers; D016212:Transforming Growth Factor beta; D001710:Biotin; D014503:Urate Oxidase