Isolation and characterization of a copper-resistant methanogen from a copper-mining soil sample.
A copper-resistant methanogen for which the CuSO4 MICs were approximately 2- to 36-fold higher than those for other methanogens tested was isolated from a copper-mining area in the upper peninsula of Michigan. The rod-shaped methanogen used H2-CO2 or formate, but not acetate or methanol, as a growth substrate. Standing incubation with H2-CO2 medium resulted in a mat-like surface growth, dependent on the presence of hydrogen. The presence of 1 mM cupric salt resulted in longer filamentous and intertwined cells. Antigenic fingerprinting, 16S rRNA gene analysis, morphology, and substrate use suggest that the new isolate is a novel strain of Methanobacterium bryantii that is able to use formate.
Kim
B K
BK
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
Conway de Macario
E
E
Nölling
J
J
Daniels
L
L
D000942:Antigens, Bacterial / Q000235:genetics; D003300:Copper / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D019605:Euryarchaeota / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D005561:Formates / Q000378:metabolism; D005798:Genes, Bacterial; D008855:Microscopy, Electron, Scanning; D008906:Mining; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D000942:Antigens, Bacterial; D005561:Formates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C030544:formic acid; D003300:Copper