Determination of morphine analogues, caffeine and amphetamine in biological fluids by capillary electrophoresis with the marker technique.
A reliable method was sought for the fast screening and simultaneous determination of amphetamine, morphine, heroin (acetomorphine), codeine (methylmorphine) and caffeine in biological fluids and drug seizures. Capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC), with detection at 200 and 220 nm, were investigated for analytes in human serum and urine. When adequate separation was not achieved in preliminary studies with CZE, further development was focused on the MEKC method. Glycine buffer containing sodium lauryl sulfate (pH 10.5) was used for the MEKC separations. The analytes and carboxylic acids used as marker compounds could be screened by a short-capillary method in less than 2 min. In the simultaneous determination of the drugs in urine and serum a longer separation of 18 min was preferred so that all the compounds, the markers and the endogenous compounds absorbing at the detection wavelength could be adequately separated in a single run. The migration times of the compounds increased in the order caffeine, morphine, heroin, codeine and amphetamine. The repeatability of the separation was tested by using two carboxylic acids as marker compounds in the determination of the migration indices of the analytes. The relative standard deviations for the migration indices were less than 1%, which is accurate enough for the determination of the drugs in biological fluids.
Hyötyläinen
T
T
Department of Chemistry, University of Helsinki, Finland.
Sirén
H
H
Riekkola
M L
ML
D000661:Amphetamine / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D002110:Caffeine / Q000032:analysis*; D002186:Cannabinoids / Q000032:analysis; D003061:Codeine / Q000032:analysis; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D003932:Heroin / Q000032:analysis; D006801:Humans; D007700:Kinetics; D008823:Micelles; D009020:Morphine / Q000032:analysis; D009022:Morphine Derivatives / Q000032:analysis*
D002186:Cannabinoids; D008823:Micelles; D009022:Morphine Derivatives; D002110:Caffeine; D003932:Heroin; D009020:Morphine; D000661:Amphetamine; D003061:Codeine