title: Detection of immobilized amplicons by ELISA-like techniques.
abstract: The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.
authors.affiliation: Nalge Nunc International, Naperville, IL 60563-1796, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Oroskar
authors.forename: S E
authors.initials: SE
authors.lastname: Rasmussen
authors.forename: H N
authors.initials: HN
authors.lastname: Rasmussen
authors.forename: S R
authors.initials: SR
authors.lastname: Rasmussen
authors.forename: B M
authors.initials: BM
authors.lastname: Sullivan
authors.forename: A
authors.initials: A
authors.lastname: Johansson
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D001909:Leukemia Virus, Bovine / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012116:Resins, Plant
chemical_list: D004279:DNA, Viral; D012116:Resins, Plant