Molecular cloning of a novel human cDNA, RT14, containing a putative ORF highly conserved between human, fruit fly, and nematode.
From a human fetal-brain cDNA library we isolated a novel gene, designated RT14, whose cDNA contained an open reading frame of 375 nucleotides encoding 125 amino acids. A 71-amino-acids region of the predicted peptide sequence showed 62% identity with a putative peptide encoded in the region close to Drosophila Tra-2. It also showed significant homology with the putative peptide of T20G5.10 of C. elegans. Northern-blot analysis revealed expression of 0.7-kb and 1.1-kb transcripts in all human tissues examined.
Watanabe
T K
TK
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
Fujiwara
T
T
Shinomiya
H
H
Kuga
Y
Y
Hishigaki
H
H
Nakamura
Y
Y
Hirai
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009348:Nematoda / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
C104378:BLOC1S1 protein, human; D009419:Nerve Tissue Proteins; D004247:DNA