Pathogenic action of Entamoeba invadens: intestinal epithelium invasion by trophozoites in vitro.
Interactions between live Entamoeba invadens trophozoites and guinea-pig caecal explants were studied. A high percentage of amoebae adhering to the apical surface of epithelial cells was observed 10-20 min after infection, but no histopathological changes were observed. After 30 min, mild oedema at the base of the interglandular epithelium and death of some epithelial cells were evident. The epithelial barrier was invaded by amoebae at desquamating zones and phagocytosis of epithelial cells or cellular debris was occasionally observed. Invasion of the mucosa and tissue necrosis became more severe with increased time of incubation. The continuity of epithelial lining was severely compromised after 2 h of infection and erosive lesions were prominent in the mucosa. These results demonstrate that E. invadens is able to invade the intestinal epithelium although it reportedly lacks the powerful cytotoxic and cytolytic elements described for E. histolytica.
Mora-Galindo
J
J
Sección de Biologia Celular, I M S S, Guadalajara, Jal. México.
Ramirez-Romo
S
S
Jauregui-Topete
M M
MM
D000818:Animals; D046508:Culture Techniques; D004747:Entamoeba / Q000472:pathogenicity*; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000469:parasitology* / Q000648:ultrastructure