title: Fluorometric determination for micro amounts of albumin and globulin fractions without separation by using alpha, beta, gamma, delta-tetra(4'-carboxyphenyl)porphin.
abstract: A simultaneous determination of micro amounts of albumin and globulin fractions by fluorometry with only reagent alpha,beta,gamma,delta-tetra(4'-carboxyphenyl)porphin (TCPP) in the aqueous solution has been developed. Under the experimental conditions (5.6 x 10(-7) mol/liter of TCPP, pH 3.0, and at room temperature), the linear range of this assay was 0.09-12 microgram/ml and the detection limits (3 S/N) were 60 ng/ml for albumin and 90 ng/ml for globulin, respectively. The human serum samples were measured satisfactorily by using this method.
authors.affiliation: Department of Chemistry, Peking University, Beijing, People's Republic of China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: K A
authors.initials: KA
authors.lastname: Li
authors.forename: S Y
authors.initials: SY
authors.lastname: Tong
mesh_terms: D016477:Artifacts; D005916:Globulins / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D011166:Porphyrins / Q000737:chemistry*; D012709:Serum Albumin / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence
chemical_list: D005916:Globulins; D011166:Porphyrins; D012709:Serum Albumin; C018395:tetracarboxyphenylporphine; D012967:Sodium Dodecyl Sulfate