Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan.
The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
Sechi
L A
LA
Institute of Microbiology and Virology, University of Sassari, Italy. microb@ssmain.uniss.it
Zanetti
S
S
Delogu
G
G
Montinaro
B
B
Sanna
A
A
Fadda
G
G
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D005069:Evaluation Studies as Topic; D005819:Genetic Markers; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000150:complications / Q000382:microbiology; D006801:Humans; D007558:Italy / Q000453:epidemiology; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology*
D017931:DNA Primers; D004251:DNA Transposable Elements; D005819:Genetic Markers