Partitioning of proteins in dextran/hydrophobically modified dextran aqueous two-phase systems.
Partitioning of proteins was studied in aqueous two-phase systems composed of the polymers dextran and hydrophobically modified dextran. The modified dextrans were benzoyl dextran with a degree of substitution of 0.17 and valeryl dextran with a degree of substitution of 0.20. Phase diagrams for the systems of dextran/benzoyl dextran and dextran/valeryl dextran were determined at room temperature. The proteins studied were beta-galactosidase, bovine serum albumin, beta-lactoglobulin, lysozyme, myoglobin and cytochrome C. The partition coefficients of a series of salts were determined in dextran/benzoyl dextran two-phase systems. The addition of salts had strong effect on the partitioning of proteins. This effect was related to protein net charge and the position of the ions in the Hofmeister series. Cross partitioning of bovine serum albumin was studied in a dextran/benzoyl dextran aqueous two-phase system.
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Department of Biochemistry, University of Lund, Sweden.
Johansson
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G
Albertsson
P A
PA
Tjerneld
F
F
D000818:Animals; D002021:Buffers; D002417:Cattle; D002645:Chickens; D003574:Cytochrome c Group / Q000302:isolation & purification / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D006736:Horses; D007526:Isoelectric Point; D007782:Lactoglobulins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism; D009211:Myoglobin / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D001616:beta-Galactosidase / Q000302:isolation & purification / Q000378:metabolism
D002021:Buffers; D003574:Cytochrome c Group; D003911:Dextrans; D007782:Lactoglobulins; D009211:Myoglobin; D011506:Proteins; D012710:Serum Albumin, Bovine; D009113:Muramidase; D001616:beta-Galactosidase