A minimal medium for growth of Pasteurella multocida.
We developed a minimal medium supporting the growth of both toxigenic and nontoxigenic strains of Pasteurella multocida to optical densities of > 0.5 (600 nm). P. multocida P1059 (ATCC 15742), one of a number of strains which can cause fowl cholera, was used as the model strain in this study. The medium was composed of 17 ingredients including cysteine, glutamic acid, leucine, methionine, inorganic salts, nicotinamide, pantothenate, thiamine, and an energy source. Leucine was not required for growth but was stimulatory, and thiamine could be replaced by adenine. An additional 46 strains of P. multocida were tested, and 40 out of 46 (87%) strains grew as well as strain P1059 through a minimum of 10 serial transfers. P. multocida toxin (PMT) was produced when cells of a known toxigenic strain (P4261) were cultivated in the minimal medium. No growth of Pasteurella Haemolytica or Pasteurella trehalosi strains was observed in this minimal medium.
Jablonski
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Department of Biological Sciences, Northern Illinois University, DeKalb 60115-2861, USA. Pjablonski@niu.edu
Jaworski
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Hovde
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D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis; D016895:Culture Media, Serum-Free / Q000737:chemistry; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary; D016979:Pasteurella multocida / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D011201:Poultry Diseases / Q000382:microbiology; D013045:Species Specificity; D014774:Virulence
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D016895:Culture Media, Serum-Free; C062258:Pasteurella multocida toxin