Primary structure of human plasma membrane Ca(2+)-ATPase isoform 3.
The complete coding sequence of the human plasma membrane calcium ATPase (PMCA) isoform 3 was determined from overlapping genomic and cDNA clones. The cDNAs for the two major alternative splice variants 3a (3CII) and 3b (3CI) code for proteins of 1173 and 1220 amino-acid residues, respectively, which show 98% identity with the corresponding rat isoforms. On a multiple human tissue Northern blot, a major PMCA3 transcript of about 7 kb was detected exclusively in the brain, demonstrating the highly restricted pattern of expression of this isoform to human neuronal tissues. With the elucidation of the human PMCA3 primary structure, complete sequence information is now available for the entire family of human PMCA isoforms.
Brown
B J
BJ
Department of Biochemistry and Molecular Biology, Mayo Graduate School, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Hilfiker
H
H
DeMarco
S J
SJ
Zacharias
D A
DA
Greenwood
T M
TM
Guerini
D
D
Strehler
E E
EE
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D000252:Calcium-Transporting ATPases / Q000737:chemistry* / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology*; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000196:embryology / Q000201:enzymology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D051381:Rats
D007527:Isoenzymes; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D000252:Calcium-Transporting ATPases