title: An intravascular microdialysis sampling system suitable for application in continuous biochemical monitoring of glucose and lactate.
abstract: Direct sensor implantation for continuous biochemical monitoring has proved disappointing, but microdialysis sampling devices can serve as a biocompatible patient-sensor interface. We propose a novel "MiniShunt" extracorporeal microdialysis sampling circuit designed to sample core blood, and report the performance of different microdialysis probe membrane types and sizes with pumped and non-pumped blood flow in anaesthetised dogs. Discrete microdialysate and plasma samples collected during glucose and lactate monitoring were analysed with a YSI analyzer and showed microdialysis efficiency (% relative recovery) in excess of 95% for both analytes using fibres of greater than 70 mm2 internal surface area. Pumped veno-venous extracorporeal blood microdialysis sampling of this type could provide a suitable interface for future multi-analyte on-line biosensor applications in critically ill patients.
authors.affiliation: Education and Research Centre, St. Vincent's Hospital, Dublin, Ireland.
authors.forename: K
authors.initials: K
authors.lastname: Rabenstein
authors.forename: A J
authors.initials: AJ
authors.lastname: McShane
authors.forename: M J
authors.initials: MJ
authors.lastname: McKenna
authors.forename: E
authors.initials: E
authors.lastname: Dempsey
authors.forename: T V
authors.initials: TV
authors.lastname: Keaveny
authors.forename: R
authors.initials: R
authors.lastname: Freaney
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D004285:Dogs; D004867:Equipment Design; D007773:Lactates / Q000032:analysis*; D008566:Membranes; D017551:Microdialysis / Q000295:instrumentation*; D008904:Miniaturization / Q000295:instrumentation; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D012044:Regression Analysis; D006435:Renal Dialysis / Q000295:instrumentation*
chemical_list: D001786:Blood Glucose; D007773:Lactates