title: Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.
abstract: We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.
authors.affiliation: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamagishi
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Oshima
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D011743:Pyrimidines / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D011743:Pyrimidines; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase