High-resolution fluorescence in situ hybridization of RBM- and TSPY-related cosmids on released Y chromatin in humans and pygmy chimpanzees.
Applying two-colour fluorescence in situ hybridization (FISH) we simultaneously hybridized RBM- and TSPY-related cosmids to Y chromosomes in prophase and to released Y chromatin in interphase nuclei of man and pygmy chimpanzee. Whereas, even on prophasic Y chromosomes, no resolution of the overlapping RBM and TSPY signal clusters could be achieved, the RBM and TSPY signals are completely separated from each other in our maximum released Y chromatin stretches in interphase nuclei. These results unequivocally lend support to the view that the RBM and TSPY families have an interspersed organization on the Y chromosomes of man and higher apes. Thus, the distribution of RBM and TSPY signals might well go back to a common organization of these genes next to each other on an ancient Y chromosome.
Conrad
C
C
Institute of Human Genetics and Anthropology, University of Freiberg, Germany.
Hierl
T
T
Gläser
B
B
Taylor
K
K
Zeitler
S
S
Chandley
A C
AC
Schempp
W
W
D000818:Animals; D018797:Cell Cycle Proteins; D003360:Cosmids / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D009687:Nuclear Proteins; D002679:Pan troglodytes / Q000235:genetics*; D012313:RNA / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D051876:Sex-Determining Region Y Protein; D013045:Species Specificity; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics* / Q000648:ultrastructure
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497749:TSPY1 protein, human; D014157:Transcription Factors; D012313:RNA