Intraplatelet free calcium and calcium-regulating hormones in plasma are not related to the antihypertensive effect of nifedipine in hypertensive pregnancy.
Intracellular free calcium regulates contraction-relaxation processes in vascular smooth muscle. We compared intraplatelet free calcium ([Ca2+]i) and pH ([pH]i) in hypertensive pregnant women to those in normotensive pregnant and non-pregnant women. Plasma parathormone and vitamin D metabolite were simultaneously assessed. In hypertensive pregnancy, [Ca2+]i tended to be lower than in normotensive pregnant (P = 0.08) and non-pregnant subjects (P = 0.06). In hypertensive pregnancy, 1,25, (OH)2 vitamin D in plasma was in the same range as in non-pregnant women and significantly lower than in normotensive pregnancy (p < 0.01). The other two vitamin D metabolites, parathormone and [pH]i were equal in the three groups. A five-day nifedipine treatment (10 mg t.i.d.) increased [Ca2+]i in hypertensive pregnant (P < 0.05) and normotensive non-pregnant subjects (P = 0.06), whereas [pH]i (P < 0.05) and 25 (OH) vitamin D (P < 0.05) decreased in the former and 24,25 (OH)2 vitamin D increased in the latter group (P < 0.05). Initial [Ca2+]i did not correlate with blood pressure in any group. The antihypertensive effect of nifedipine did not correlate with any variable measured. In conclusion, [Ca2+]i and calcium-regulating hormones seem not to be related to the antihypertensive effect of nifedipine in hypertensive pregnancy. In this type of hypertension, intraplatelet calcium may not reflect calcium balance in smooth muscle cells regulating vascular tone and blood pressure.
Manninen
A
A
Medical School, University of Tampere, Finland.
Wuorela
H
H
Laippala
P
P
Vapaatalo
H
H
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003270:Contraceptive Agents / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000097:blood / Q000188:drug therapy*; D009543:Nifedipine / Q000494:pharmacology*; D010281:Parathyroid Hormone / Q000097:blood; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000188:drug therapy*; D014807:Vitamin D / Q000378:metabolism
D002121:Calcium Channel Blockers; D003270:Contraceptive Agents; D010281:Parathyroid Hormone; D014807:Vitamin D; D009543:Nifedipine; D002118:Calcium