title: Oscillatory patterns of electrical activity in mouse pancreatic islets of Langerhans recorded in vivo.
abstract: Pancreatic beta-cells secrete insulin as a function of blood glucose concentration. One of the key steps in stimulus-secretion coupling is the depolarisation of the membrane and the appearance of bursts of calcium action potentials. Recently, the characteristics and glucose dependence of the oscillations in electrical activity in vivo have been described. The experiments described here were designed to determine the temporal evolution of such electrical activity when no experimental changes in the glycaemia are imposed. The absolute duration of the active and silent phases has been analysed and compared with the values obtained in vitro. We have found that in vivo, at glycaemia ranging from 6.0 to 7.5 mM, the electrical activity of the islets of Langerhans is permanently oscillatory, the mean duration of the depolarisation phase being 28 s. In general, the oscillatory pattern remains very constant for relatively long (up to 60 min) periods of time. In some experiments, slow or transitory changes in the degree of beta-cell activation could be observed, as well as the existence, in a very few cases, of oscillatory non-periodic patterns. Key words beta-cells middle dot Pancreas middle dot Electrophysiology middle dot Oscillations
authors.affiliation: Instituto de Neurociencias, Facultad de Medicina, Universidad de Alicante, E-03080 Alicante, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gomis
authors.forename: J V
authors.initials: JV
authors.lastname: Sánchez-Andrés
authors.forename: M
authors.initials: M
authors.lastname: Valdeolmillos
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D004594:Electrophysiology; D007515:Islets of Langerhans / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques; D013997:Time Factors
chemical_list: D001786:Blood Glucose