A technique to assess aortic distensibility and compliance in anesthetized and awake rats.
A noninvasive ultrasonic technique, based on tracking arterial wall displacements with a vessel wall-tracking device attached to a conventional B-mode imager, to assess end-diastolic aortic diameter (d) and aortic diameter changes during the cardiac cycle (delta d) in anesthetized and awake rats is presented. From these parameters and invasively measured aortic pulse pressure (delta P), aortic distensibility and compliance, the relative and absolute increases in lumen cross-sectional area for a given increase in delta P, respectively, can be calculated. d, delta d, and delta P could be determined with good intra-session (variations per day) and inter-session (variations between days) coefficients of variation (CV). The CVs for delta d were smaller in awake (4.6-6.0%) than in anesthetized rats (7.9-11.0%), probably due to variations in delta P during anesthesia (CV: 9.0-12.3%). The CVs for d in awake (3.3-6.5%) and anesthetized rats (2.6-5.0%) were comparable. In awake rats the CV for delta d, but not for d, increased after implantation of the aortic catheter. It is concluded that d, delta d, and delta P of the aorta can be reliably measured noninvasively in anesthetized and awake rats, allowing the in vivo assessment of aortic distensibility and compliance. The technique is sensitive enough to detect effects of agents on aortic wall properties.
Van Gorp
A
A
Department of Physiology, University of Limburg, Maastricht, The Netherlands.
Van Ingen Schenau
D S
DS
Willigers
J
J
Hoeks
A P
AP
De Mey
J G
JG
Struyker Boudier
H A
HA
Reneman
R S
RS
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000758:Anesthesia; D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects; D002309:Cardiology / Q000379:methods*; D003187:Compliance; D004548:Elasticity; D005069:Evaluation Studies as Topic; D008729:Methoxamine / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D014463:Ultrasonography; D014851:Wakefulness
D000316:Adrenergic alpha-Agonists; D008729:Methoxamine