Luminol-enhanced, whole blood chemiluminescence of human neutrophils evaluated by means of an automated, computer-assisted, and high-sensitivity luminescence analyzer.
Luminol-enhanced whole blood chemiluminescence of human neutrophils was studied using opsonized zymosan as a stimulus. Heparinized blood (0.5 microliters) was used, and the chemiluminescence signals were recorded by a very sensitive, automated, and computer-assisted luminometer (LB 950, Berthold, Wildbad, Germany). The following parameters were provided: integral values over the total measuring time, peak values, the time to reach maximum value, and the time to reach half maximum value. Normal subjects, neutropenic patients, subjects with total or partial myeloperoxidase deficiency, patients with recurrent infections, phagocytic defects, thrombocythemic patients and those with non-Hodgkin's lymphomas undergoing therapy with recombinant human granulocyte colony-stimulating factor were studied. The integral response of chemiluminescence and the time of reach half maximal value were useful indicators of chemiluminescence defects; the assay, was able to detect chemiluminescence responses in neutropenic subjects with neutrophil levels as low as 0.6 x 10(9)/l; differences between cellular and plasma defects could be identified; the quenching effect exerted by erythrocytes was negligible.
Carulli
G
G
Hematology Unit, University of Pisa, Italy.
Minnucci
S
S
Angiolini
C
C
Azzarà
A
A
Ambrogi
F
F
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D001330:Electronic Data Processing / Q000295:instrumentation / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007223:Infant; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D009503:Neutropenia; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D016897:Respiratory Burst / Q000276:immunology; D012141:Respiratory Tract Infections / Q000276:immunology; D012680:Sensitivity and Specificity; D012984:Software
D008165:Luminol