title: Isolation and subcloning of chitinase clone from chickpea genomic library.
abstract: Chickpea genomic library constructed earlier in phage lambda (EMBL-3) was screened for the presence of chitinase clone using tobacco chitinase cDNA as a probe. Positive clones obtained by primary screening of plaques (2 x 10(6)) were ascertained by secondary and tertiary screening. Presence of chitinase insert in the positive clones obtained, was further confirmed by restricting phage DNA with Sal I and then doing southern with tobacco chitinase. The insert band was eluted out and subcloned in puc 19 plasmid.
authors.affiliation: National Research Centre on Plant Biotechnology, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Grover
authors.forename: A
authors.initials: A
authors.lastname: Tyagi
authors.forename: K R
authors.initials: KR
authors.lastname: Koundal
authors.forename: R P
authors.initials: RP
authors.lastname: Sharma
mesh_terms: D002688:Chitinases / Q000302:isolation & purification*; D003001:Cloning, Molecular; D007887:Fabaceae / Q000201:enzymology*; D018745:Genome, Plant; D015698:Genomic Library; D010946:Plants, Medicinal
chemical_list: D002688:Chitinases