Lymphocyte subpopulations after extensive small bowel resection in the rat.
The increased risk of infection after massive intestinal resection (MIR) may be attributable to impaired nutrition, loss of intestinal lymphoid tissue, or both. This study examines whether MIR itself changes the immune cell populations in laboratory animals when nutritional status is preserved. The authors studied cellular immunity (lymphocyte subsets T4 and T8 and the T4:T8 ratio) and humoral immunity (IgG, IgM, IgA, and B lymphocytes) in the blood, spleen, and mesenteric lymph nodes of unresected Wistar rats (control group, n = 6) and of animals that underwent 80% bowel resection followed by 7 days of either oral feeding (resection-oral group, n = 6) or parenteral nutrition (resection-TPN group, n = 6). The increase in body weight was similar among all groups, and the levels of total protein, albumin, prealbumin, and immunoglobulin remained unchanged. All resected animals, irrespective of their feeding route, had significantly lower proportions of T4 and B lymphocytes and T4:T8 ratio in blood, T4 and T8 in mesenteric lymph nodes, and T4 and T4:T8 ratio in the spleen. The author's results suggest that removal of large amounts of lymphoid tissue along with the bowel during MIR might lead to inadequate immune response even when the nutritional status is preserved.
Barrena
M J
MJ
Hospital N.S. Aranzazu, Universidad del País Vasco, San Sebastián, Spain.
Eizaguirre
I
I
Aldazabal
P
P
Cuadrado
E
E
Bachiller
P
P
Wang
W
W
Tovar
J A
JA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007421:Intestine, Small / Q000601:surgery*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M