Association between genetic variants of mast-cell chymase and eczema.
BACKGROUND
Atopy is a common syndrome underlying asthma, rhinitis, and eczema, and is characterised by high immunoglobulin E (IgE) responses to common antigens. IgE and mast-cell chymase (MCC-a serine protease secreted by skin mast cells) have a key role in atopic or allergic inflammation of the skin. The gene for MCC is located within a cluster of genes for cellular proteases on chromosome 14q11.2. We aimed to identify variants of MCC and another gene within this complex, and assess whether there is a genetic association between variants of MCC and atopic disorders-particularly eczema.


METHODS
We randomly selected 100 controls and recruited patients-100 in each group-with atopic asthma, non-atopic asthma, atopic rhinitis, and atopic eczema. PCR amplification was used to test genomic DNA for an association between allelic polymorphisms in MCC and a flanking gene (CGL1, for the cathepsin-G-like protein) on chromosome 14q11 and asthma, rhinitis, and eczema.


RESULTS
We found a significant association between a BstXI polymorphism in MCC and eczema (odds ratio 2.17 [95% CI 1.21-3.88], p = 0.009), but no association with atopic asthma, rhinitis, or non-atopic asthma. There was no association between an Mboll polymorphism in CGL1 and any of the atopic disorders.


CONCLUSIONS
These findings suggest that variants of MCC may be one source of genetic risk for eczema.
Mao
X Q
XQ
Department of Hygeine and Preventive Medicine, Osaka University School of Medicine, Suita, Japan.
Shirakawa
T
T
Yoshikawa
T
T
Yoshikawa
K
K
Kawai
M
M
Sasaki
S
S
Enomoto
T
T
Hashimoto
T
T
Furuyama
J
J
Hopkin
J M
JM
Morimoto
K
K
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000235:genetics; D001345:Autoradiography; D001483:Base Sequence; D016022:Case-Control Studies; D053818:Chymases; D004485:Eczema / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D012220:Rhinitis / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics*
D012697:Serine Endopeptidases; D053818:Chymases