Role of the NHE3 isoform of the Na+/H+ exchanger in sodium absorption by the rabbit gallbladder.
The absorption of water and electrolytes by the gallbladder seems to be largely dependent upon a Na+/H+ exchange at the apical membrane of the gallbladder epithelium. To find out if the exchanger involved is the NHE3 isoform, as in other absorbing epithelia, two studies were performed using the rabbit gallbladder. First, we studied 22Na absorption in Ussing chambers with Krebs buffer as a control solution, and in the presence of amiloride (100, 200 or 1000 microM), ethyl-isopropyl-amiloride (EIPA, 1 or 5 microM), or the phorbol ester, phorbol 12-myristate 13-acetate (PMA, 1 microM). A net mucosal-to-serosal Na+ flux was observed with control buffer. No inhibition of this net flux was observed with 5 microM EIPA, and the IC50 for amiloride was found to be 200 microM. PMA induced a reduction of absorption by 30% that was prevented by incubation with calphostin C. Resistance to amiloride and EIPA, and inhibition by PMA are consistent with the involvement of the NHE3 isoform. The second study involved reverse-transcriptase polymerase chain reaction (RT-PCR) of total gallbladder RNA, with two primers designed to amplify a 645-base-pair fragment from NHE3 mRNA. A cDNA fragment of the expected size was actually obtained from gallbladder RNA, while RT-PCR of RNA from the liver, which does not contain NHE3, gave negative results. A sequence of 492 nucleotides of the amplified product was determined, which was almost superimposable onto the known sequence of the corresponding fragment of rabbit NHE3. It is concluded that, in rabbit gallbladder, neutral NaCl absorption is, at least in part, dependent on the NHE3 isoform of the Na+/H+ exchanger.
Silviani
V
V
Inserm U 260, Faculté de Médecine 27 Bd Jean Moulin, F-13385 Marseille, Cedex 05, France.
Colombani
V
V
Heyries
L
L
Gerolami
A
A
Cartouzou
G
G
Marteau
C
C
D000042:Absorption; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001834:Body Water / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005704:Gallbladder / Q000378:metabolism*; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D009281:Naphthalenes; D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D012964:Sodium; C058819:calphostin C; D013755:Tetradecanoylphorbol Acetate