title: Epidermal Langerhans' cell induction of immunity against an ultraviolet-induced skin tumour.
abstract: Langerhans' cells (LC) have been shown experimentally to induce immune responses against many antigens; however, their role in the initiation of anti-tumour immunity has received little attention. This study examined the ability of murine epidermal LC to induce immunity to an ultraviolet radiation (UV)-induced skin tumour. Freshly prepared epidermal cells (EC) were cultured for 2 or 20 hr with granulocyte-macrophage colony-stimulating factor (GM-CSF), pulsed with an extract of the UV-13-1 tumour, then used to immunize naive syngeneic mice. Delayed type hypersensitivity (DTH) was elicited 10 days after immunization by injection of UV-13-1 tumour cells into the ear pinna, and measured 24 hr later. EC cultured with GM-CSF for 2 hr induced antitumour DTH, as did EC cultured for 20 hr without GM-CSF. Conversely, EC cultured for 2 hr without GM-CSF, or EC cultured for 20 hr with GM-CSF were unable to induce a DTH. Induction of immunity required active presentation of tumour antigens by Ia+ EC and was tumour specific. Thus Ia+ epidermal cells are capable of inducing anti-tumour immunity to UV-induced skin tumours, but only when they contact antigen in particular states of maturation.
authors.affiliation: Department of Medicine, University of Sydney at Royal Prince Alfred Hospital, NSW, Australia.
authors.forename: L L
authors.initials: LL
authors.lastname: Cavanagh
authors.forename: R
authors.initials: R
authors.lastname: Sluyter
authors.forename: K G
authors.initials: KG
authors.lastname: Henderson
authors.forename: R S
authors.initials: RS
authors.lastname: Barnetson
authors.forename: G M
authors.initials: GM
authors.lastname: Halliday
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D007801:Langerhans Cells / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009381:Neoplasms, Radiation-Induced / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000949:Histocompatibility Antigens Class II; D016178:Granulocyte-Macrophage Colony-Stimulating Factor