A standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of the European sea bass Dicentrarchus labrax L.: comparison of extruded and extruded/coated diets.
The stability of the coated lipid fraction of a standard weaning diet was verified by comparing growth and fatty acid incorporation for European sea bass fed diets with an identical formulation but prepared either by extrusion/coating or extrusion only. For each type of experimental diet, a (n-3) HUFA level of 1 and 2.5% of the dry diet was evaluated. European sea bass Dicentrarchus labrax L. were weaned and consequently reared for 23 days on the experimental diets in a recirculation system. A commercial weaning diet served as a control. Final individual dry weight of sea bass larvae weaned and ongrown for a total period of 30 days on the various diets did not differ significantly. The (n-3) HUFA content of the fish tissue was a reflection of the dietary (n-3) HUFA requirements of European sea bass during and immediately after weaning did not exceed 1% of the dry diet. The combined technique of cooking-extrusion of a basal diet followed by coating of a lipid emulsion proved to be a valid technique to prepare diets for studying quantitative fatty acid requirements of weaning and first ongrowing stages of marine fish. A standard diet with open formulation is proposed.
Coutteau
P
P
Laboratory of Aquaculture & Artemia Reference Center, University of Ghent, Gent, Belgium.
Van Stappen
G
G
Sorgeloos
P
P
D000375:Aging / Q000378:metabolism / Q000502:physiology*; D000704:Analysis of Variance; D000821:Animal Feed / Q000592:standards; D000818:Animals; D001492:Bass / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D001530:Belgium; D004032:Diet / Q000592:standards / Q000662:veterinary*; D004435:Eating / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology* / Q000592:standards; D015525:Fatty Acids, Omega-3 / Q000378:metabolism / Q000494:pharmacology / Q000592:standards; D005398:Fisheries; D005511:Food Handling / Q000379:methods*; D050356:Lipid Metabolism; D008055:Lipids / Q000494:pharmacology / Q000592:standards; D009751:Nutritional Requirements; D014886:Weaning
D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D008055:Lipids