Renaturation and reconstitution of functional holoenzyme from recombinant subunits of casein kinase II expressed as inclusion bodies in E. coli.
Lin
W J
WJ
Department of Biochemistry, University of California, Riverside 92521, USA.
Jakobi
R
R
Traugh
J A
JA
D000818:Animals; D047390:Casein Kinase II; D003001:Cloning, Molecular; D004229:Dithiothreitol / Q000494:pharmacology; D004330:Drosophila / Q000201:enzymology; D004926:Escherichia coli; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D006801:Humans; D002479:Inclusion Bodies; D007700:Kinetics; D046911:Macromolecular Substances; D011489:Protein Denaturation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D005978:Glutathione; D004229:Dithiothreitol; D019803:Glutathione Disulfide