Mutations Modulating Raf signaling in Drosophila eye development.
The R7 fate is specified during Drosophila eye development by an inductive signal transduced intracellularly via the Raf kinase. We have performed a genetic screen for dominant mutations that alter the efficiency with which cells respond to a constitutively activated Raf kinase. Such mutations may affect genes involved in signal transduction downstream of Raf. We have isolated 44 mutations that define eight genes. One of these encodes a mitogen-activated protein kinase homologue: another is a putative target gene of this signaling pathway. We present the results of this screen in detail, as well as a preliminary genetic analysis of the six loci still to be characterized molecularly.
Dickson
B J
BJ
Zoologisches Institut, Universität Zũrich, Switzerland.
van der Straten
A
A
Dominguez
M
M
Hafen
E
E
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D004330:Drosophila / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D005123:Eye / Q000254:growth & development* / Q000648:ultrastructure; D005260:Female; D017344:Genes, Insect; D005816:Genetic Complementation Test; D006720:Homozygote; D008297:Male; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf; D015398:Signal Transduction / Q000235:genetics
D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf