Effect of age of donor on the responsiveness of dispersed and cultured chicken anterior pituitary cells to GnRH-I.
1. The aim of this study was to devise a method to prepare and culture anterior pituitary cells from juvenile and adult chickens in order to investigate mechanisms controlling gonadotrophin-releasing hormone-I (GnRH-I)-induced luteinising hormone (LH) release in vitro. 2. The optimum culture medium for maintaining gonadotroph responsiveness to GnRH-I was bicarbonate-buffered and phenol red-free Medium 199 supplemented with 10% foetal calf serum. 3. Cultured pituitary cells from juvenile chickens were more responsive to GnRH-I than cells from adult cockerels, while no LH was released in response to GnRH-I from pituitary cells from laying hens. 4. Cultured pituitary cells from adult chickens of both sexes released LH in response to 12-O-tetradecanoyl-13-phorbol acetate (TPA), an activator of an enzyme involved in intracellular signalling, protein kinase C. 5. It is concluded that freshly-dispersed and cultured gonadotrophs from adult chickens do not regain their responsiveness to GnRH-I as well as freshly-dispersed and cultured gonadotrophs from juvenile chickens. It appears that the stimulus-secretion coupling pathway between the GnRH-receptor and the activation of protein kinase C in gonadotrophs from adult chickens is more easily disrupted by dispersion and culture than in gonadotrophs from juvenile chickens.
Liu
R C
RC
Department of Applied Biology, University of Central Lancashire, Preston, England.
Sharp
P J
PJ
Lea
R W
RW
D000375:Aging / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D010058:Oviposition; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate