title: Structure of amylovoran, the capsular exopolysaccharide from the fire blight pathogen Erwinia amylovora.
abstract: The acidic exopolysaccharide (EPS) of Erwinia amylovora, amylovoran, was purified from culture supernatants of bacteria in minimal medium and cleaved chemically either by treatment with trifluoracetic acid or hydrofluoric acid, and enzymatically by digestion with depolymerase from E. amylovora phage phi-Ealh. Structural characterization of the resulting oligosaccharides was performed by a combination of mass spectrometric and NMR [one- and two-dimensional (1D and 2D)] spectroscopic techniques. A branched repeating unit with five monosaccharide residues and various substituents was determined: [sequence: see text] The terminal monosaccharide of the side branch, which bears a 4,6-bound pyruvate residue in the R-configuration, was found to be modified with 2-linked (26%), 3-linked (24%), 2-,3-linked (40%) O-acetyl groups, or these were absent (10%). An additional glucose residue is linked to approximately 10% of the core alpha-galactose of the repeating unit.
authors.affiliation: GBF, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Nimtz
authors.forename: A
authors.initials: A
authors.lastname: Mort
authors.forename: T
authors.initials: T
authors.lastname: Domke
authors.forename: V
authors.initials: V
authors.lastname: Wray
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Qiu
authors.forename: D
authors.initials: D
authors.lastname: Coplin
authors.forename: K
authors.initials: K
authors.lastname: Geider
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004885:Erwinia / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; C100769:amylovoran