title: Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer.
abstract: Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybrydization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T-cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.
authors.affiliation: Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Shpitz
authors.forename: H
authors.initials: H
authors.lastname: Stern
authors.forename: J S
authors.initials: JS
authors.lastname: Sandhu
authors.forename: R M
authors.initials: RM
authors.lastname: Reilley
authors.forename: M B
authors.initials: MB
authors.lastname: Tropak
authors.forename: G
authors.initials: G
authors.lastname: Jansz
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: S
authors.initials: S
authors.lastname: Gallinger
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000633:toxicity*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002469:Cell Separation; D003110:Colonic Neoplasms / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000145:classification; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes