title: Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus.
abstract: The structural polypeptides of the isolate VR-2332 of porcine reproductive and respiratory syndrome virus were analyzed in sucrose gradient-purified virions. The virus had an average density of 1.15 g/cm3 and contained, by SDS-PAGE, three major polypeptides with apparent molecular weights of 15, 19 and 26-30 kDa, which were designated as nucleocapsid (N), matrix (M) and envelope (E), respectively. The predominant structural protein was N. N-glycosidase F digestion only affected E whereas O-glycosidase or endoglycosidase H digestion had no effect, suggesting that the viral glycoproteins contain only complex N-linked carbohydrates.
authors.affiliation: Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Bautista
authors.forename: J J
authors.initials: JJ
authors.lastname: Meulenberg
authors.forename: C S
authors.initials: CS
authors.lastname: Choi
authors.forename: T W
authors.initials: TW
authors.lastname: Molitor
mesh_terms: D018018:Arterivirus / Q000737:chemistry*; D008970:Molecular Weight; D015678:Viral Structural Proteins / Q000032:analysis*
chemical_list: D015678:Viral Structural Proteins