Microsatellites and PCR genomic analysis.
Microsatellites form a significant proportion of the growing family of repetitive DNA sequences, widely dispersed in the human genome. Due to their ubiquity, PCR (polymerase chain reaction) typability, Mendelian co-dominant inheritance, and extreme polymorphism, microsatellites have assumed an increasingly important role as markers in the genome. Apart from their obvious applications in genome mapping and positional cloning, these markers have been applied in fields as disparate as tumour biology, personal identification, population genetic analysis, and the construction of human evolutionary trees. Microsatellites are associated with human disease, not only as markers of risk but also directly in disease aetiopathogenesis, providing new insights into non-Mendelian inheritance; the replication, repair, and mutation of eukaryotic DNA; the regulation of gene transcription; and protein-protein interactions. These insights have resulted in novel paradigms for oncogenesis and neurological disease.
Koreth
J
J
Nuffield Department of Pathology and Bacteriology, University of Oxford, John Radcliffe Hospital, UK.
O'Leary
J J
JJ
O'D McGee
J
J
D002874:Chromosome Mapping; D005260:Female; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005819:Genetic Markers; D016678:Genome; D016045:Human Genome Project; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction
D005819:Genetic Markers