title: Evidence for a megareplicon covering megabases of centromeric chromosome segments.
abstract: We have analysed the replication of the heterochromatic megachromosome that was formed de novo by a large-scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons approximately 30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes.
authors.affiliation: Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Holló
authors.forename: J
authors.initials: J
authors.lastname: Keresö
authors.forename: T
authors.initials: T
authors.lastname: Praznovszky
authors.forename: I
authors.initials: I
authors.lastname: Cserpán
authors.forename: K
authors.initials: K
authors.lastname: Fodor
authors.forename: R
authors.initials: R
authors.lastname: Katona
authors.forename: E
authors.initials: E
authors.lastname: Csonka
authors.forename: K
authors.initials: K
authors.lastname: Fátyol
authors.forename: A
authors.initials: A
authors.lastname: Szeles
authors.forename: A A
authors.initials: AA
authors.lastname: Szalay
authors.forename: G
authors.initials: G
authors.lastname: Hadlaczky
mesh_terms: D000818:Animals; D002503:Centromere / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics*; D004261:DNA Replication; D005784:Gene Amplification; D006822:Hybrid Cells / Q000648:ultrastructure*; D051379:Mice / Q000235:genetics*; D008957:Models, Genetic; D012093:Replicon