title: The complete nucleotide sequence of odontoglossum ringspot virus (Cy-1 strain) genomic RNA.
abstract: The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot virus Cy-1 strain (ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K, 183 K, 31 K, and 18 K proteins. Its genomic organization is similar to other tobamoviruses, TMV-V(vulgare), TMV-L (tomato strain), tobacco mild green mosaic virus (TMGMV) and cucumber green mottle mosaic virus (CGMMV). The 5' non-coding regions of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The ORFs encoding the 126 K and 183 K proteins have 61% and 63% identities with those of TMV-V. The third ORF encoded a 31 K protein homologous to TMV cell-to-cell movement protein. It has 63% identities with that of TMV-V. The fourth ORF encoded an 18 K coat protein. The 5' non-coding region, which extends from base 1 to 62 has 2 G residues and a ribosome binding site (AUU). The 3' non-coding region, 414 nucleotides in length, is entirely different from that of other tobamoviruses.
authors.affiliation: NODAI Research Institute, Tokyo University of Agriculture, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: Y
authors.initials: Y
authors.lastname: Isomura
authors.forename: Y
authors.initials: Y
authors.lastname: Matumoto
authors.forename: M
authors.initials: M
authors.lastname: Chatani
authors.forename: N
authors.initials: N
authors.lastname: Inouye
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D017898:Tobamovirus / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins