Specific binding component of the "inactive" stereoisomer (S,S)-[125I] IQNB to rat brain muscarinic receptors in vivo.
In vivo nonspecific binding can be estimated using the inactive stereoisomer of a receptor radioligand. However, the binding of the inactive stereoisomer may be partially specific. Specific binding of the inactive (S,S)-[125I]IQNB was estimated from the inhibition induced by a competing nonradioactive ligand. This technique differed from the usual approach, since it was used to study the inactive rather than the active stereoisomer. The results indicate that there is substantial specific binding for (S,S)-[125I]IQNB.
Boulay
S F
SF
Department of Radiology, George Washington University Medical Center, Washington DC 20037, USA.
McRee
R C
RC
Cohen
V I
VI
Sood
V K
VK
Zeeberg
B R
BR
Reba
R C
RC
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D011336:Probability; D011813:Quinuclidinyl Benzilate / Q000378:metabolism* / Q000493:pharmacokinetics; D011869:Radioligand Assay / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000378:metabolism; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate