Double minutes in the papillary thyroid cancer cell line PTC-1113A.
The cell line PTC-1113A was established from a metastasizing recurrent papillary thyroid cancer. The cell line was growing as monolayer and showed a complex karyotype with chromosome numbers ranging from 30 to 140/metaphase. A proportion of metaphases contained double minutes and/or pulverized chromosomes. Extrachromosomal DNA seemed to originate from a B-group chromosome. A chromosome 4 painting probe hybridized to extrachromosomal material, representing double minutes (dmin) and possibly minutes. In addition, fluorescence in situ hybridization (FISH) with the chromosome 4 library detected a translocation chromosome and a pulverized chromosome originating from chromosome 4. PTC-1113A is, to our knowledge, the single papillary thyroid cancer cell line demonstrating evidence of gene amplification.
Herrmann
M E
ME
Department of Surgery and Pathology, SUNY at Stony Brook, USA.
Lalley
P A
PA
Rydstedt
L L
LL
Meese
E
E
Lang
C H
CH
Abumrad
N N
NN
Moll
U M
UM
Talpos
G B
GB
D000368:Aged; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology*; D002877:Chromosomes, Human / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009362:Neoplasm Metastasis; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured