Isolation and subcloning of chitinase clone from chickpea genomic library.
Chickpea genomic library constructed earlier in phage lambda (EMBL-3) was screened for the presence of chitinase clone using tobacco chitinase cDNA as a probe. Positive clones obtained by primary screening of plaques (2 x 10(6)) were ascertained by secondary and tertiary screening. Presence of chitinase insert in the positive clones obtained, was further confirmed by restricting phage DNA with Sal I and then doing southern with tobacco chitinase. The insert band was eluted out and subcloned in puc 19 plasmid.
Grover
A
A
National Research Centre on Plant Biotechnology, New Delhi, India.
Tyagi
A
A
Koundal
K R
KR
Sharma
R P
RP
D002688:Chitinases / Q000302:isolation & purification*; D003001:Cloning, Molecular; D007887:Fabaceae / Q000201:enzymology*; D018745:Genome, Plant; D015698:Genomic Library; D010946:Plants, Medicinal
D002688:Chitinases