Flow cytometric analysis of T4:T8 splenic cell during dengue virus infection of mice.
The present study was undertaken to investigate the effect of dengue type 2 virus (DV) and DV-induced cytokines (CF and CF2) on T lymphocyte subpopulations of spleen by flow cytometry. Following DV-ic inoculation in mice the percent number of CD4+ and CD8+ lymphocytes in the spleen was reduced, the peak reduction in both was observed on the 6th day post-inoculation (p.i.). Intravenous inoculation of CF or CF2 in mice also decreased the percent number of CD4+ as well as CD8+ T lymphocytes subpopulation in the spleen, the maximum reduction being observed at 1 and 2 hr, respectively. The reduction in T lymphocyte subpopulation by CF and CF2 was found to be dose dependent. Thus, the alterations of T lymphocyte subpopulations during DV infection are mediated via cytokines.
Dhawan
R
R
Postgraduate Department of Microbiology, K G Medical College, Lucknow, India.
Mukerjee
R
R
Chaturvedi
U C
UC
Khare
S D
SD
D000818:Animals; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000096:biosynthesis / Q000494:pharmacology; D003715:Dengue / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology / Q000473:pathology
D016207:Cytokines; C087354:dengue-2 virus-induced cytotoxic factor