title: Glutathione is an important antioxidant molecule in the yeast Saccharomyces cerevisiae.
abstract: The tripeptide gamma-L-glutamyl-L-cystinylglycine (glutathione) is one of the major antioxidant molecules of cells and is thought to play a vital role in buffering the cell against reactive oxygen species and toxic electrophiles. We wished to determine the role of glutathione in the protection of the yeast Saccharomyces cerevisiae against oxidative stress. This study shows that glutathione is an important antioxidant molecule in yeast, with gamma-glutamylcysteine synthetase (gsh1) mutants, deficient in glutathione synthesis, being hypersensitive to H2O2 and superoxide anions in both exponential- and stationary-phase cultures. Despite this, these mutants are still able to induce adaptive stress responses to oxidants.
authors.affiliation: Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, UK.
authors.forename: D W
authors.initials: DW
authors.lastname: Stephen
authors.forename: D J
authors.initials: DJ
authors.lastname: Jamieson
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005721:Glutamate-Cysteine Ligase / Q000235:genetics; D005978:Glutathione / Q000502:physiology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D018384:Oxidative Stress; D010545:Peroxides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010545:Peroxides; D013481:Superoxides; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione