title: Epitope analysis of soybean major allergen Gly m Bd 30K recognized by the mouse monoclonal antibody using overlapping peptides.
abstract: The epitopes of the major soybean allergen, Gly m Bd 30K, recognized by mouse monoclonal antibodies H6 and F5 were investigated by using synthetic peptides bound to pins. The epitopes are shown to be localized in peptide 31QGGCGRGWAFSATGAIEA48 for H6, and in 115DKVTIDGYETLIMSDEST132 for F5.
authors.affiliation: Allergen-Free Technology Laboratories Inc., Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hosoyama
authors.forename: A
authors.initials: A
authors.lastname: Obata
authors.forename: N
authors.initials: N
authors.lastname: Bando
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D052179:Antigens, Plant; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry* / Q000276:immunology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D000939:Epitopes; C103236:Gly m BD 30K protein, Glycine max; D010940:Plant Proteins; D030262:Soybean Proteins