Detection and in vivo transmission of rat orphan parvovirus (ROPV).
Antibodies to rodent parvovirus were detected by the immunofluorescent antibody (IFA) test but not by haemagglutination inhibition (HAI) in a commercial rat breeding colony. These antibodies were considered to be a response to so-called 'orphan parvovirus'. The virus was transmissible by intraperitoneal inoculation of infected materials (spleen cell or lymphoid tissue homogenate), and direct contact with infected rats or contaminated bedding. Furthermore, parvoviral-specific DNA sequence coded non-structural (NS) protein was detected by the polymerase chain reaction (PCR) in spleen cells and peripheral lymphocytes of experimentally infected rats.
Ueno
Y
Y
Laboratory Animal Research Center, University of Tsukuba, Ibaraki, Japan.
Sugiyama
F
F
Yagami
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D016358:Contact Tracing / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006799:Housing, Animal; D008297:Male; D010322:Parvoviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012376:Rodent Diseases / Q000635:transmission* / Q000821:virology; D012698:Serologic Tests
D000914:Antibodies, Viral