title: A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus.
abstract: We identified a DNA polymerase with properties that differed from those of alpha-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.
authors.affiliation: Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Imamura
authors.forename: T
authors.initials: T
authors.lastname: Uemori
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Ishino
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000302:isolation & purification*; D054306:Dideoxynucleotides; D005033:Ethylmaleimide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors; D013379:Substrate Specificity; D013696:Temperature; D013822:Thermoplasma / Q000201:enzymology*; D013942:Thymine Nucleotides / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D054306:Dideoxynucleotides; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D016590:Aphidicolin; D004259:DNA-Directed DNA Polymerase; C017216:2',3'-dideoxythymidine triphosphate; D005033:Ethylmaleimide