Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach.
Two new genes were identified and mapped by searching the EST databases with genomic sequences obtained from putative CpG islands of the rodent-human hybrid X3000. Previous mapping of these CpG islands in the proximity of the host cell factor (HCFC1) and GdX genes automatically localized these two new genes to Xq28 in the interval between the L1 cell adhesion molecule (L1CAM) and the glucose-6-phosphate dehydrogenase (G6PD) loci. Both genes are relatively short, contain an ORF of 261 and 105 amino acids, respectively, and are ubiquitously expressed. Combining sequencing of selected CpG islands, derived from hybrids containing small portions of the human genome, with an EST database search is an easy method of identifying and mapping new genes to specific regions of the genome.
Faranda
S
S
Istituto di Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche, via Ampère 56, Milan, 20131, Italy.
Frattini
A
A
Zucchi
I
I
Patrosso
C
C
Milanesi
L
L
Montagna
C
C
Vezzoni
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003062:Codon; D017931:DNA Primers; D015226:Dinucleoside Phosphates; D005260:Female; D015894:Genome, Human; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007256:Information Systems; D039841:Leukocyte L1 Antigen Complex; D008297:Male; D008969:Molecular Sequence Data; D019006:Neural Cell Adhesion Molecules / Q000235:genetics; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012377:Rodentia; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
D003062:Codon; D017931:DNA Primers; D015226:Dinucleoside Phosphates; D005954:Glucosephosphate Dehydrogenase; D039841:Leukocyte L1 Antigen Complex; D019006:Neural Cell Adhesion Molecules; C015772:CpG dinucleotide