title: Coordinate expression of nuclear and mitochondrial genes involved in energy production in carcinoma and oncocytoma.
abstract: The expression of mitochondrial and nuclear genes involved in ATP production was examined in renal carcinomas, renal oncocytomas, and a salivary oncocytoma. Renal carcinomas were found to have a reduced mitochondrial DNA (mtDNA) content while oncocytomas had increased mtDNA contents. This parallels morphological changes in mitochondrial number in these tumours. In the carcinomas, mtDNA transcripts were decreased 5- to 10-fold relative to control kidneys, suggesting that mitochondrial transcript levels depend on the mtDNA content. In renal oncocytomas, mtDNA transcripts were slightly reduced in spite of a high mtDNA content. However, in the salivary gland oncocytoma, mtDNA transcripts were increased more than 10-fold in parallel with a 10-fold increase in mtDNA content. The expression of the nuclear DNA oxidative phosphorylation genes, ATPsyn beta and ANT2, was reduced up to 4-fold in renal carcinoma. In contrast, the levels of these two nuclear gene transcripts were induced about 4-fold in renal oncocytoma and up to 30-fold in salivary gland oncocytoma. Moreover, the ANT2 precursors were observed to change in oncocytomas. These data suggest a coordinated regulation of nuclear and mitochondrial gene expression in renal carcinomas and the specific induction of nuclear OXPHOS gene expression in oncocytomas.
authors.affiliation: Department of Genetics and Molecular Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: A
authors.initials: A
authors.lastname: Heddi
authors.forename: H
authors.initials: H
authors.lastname: Faure-Vigny
authors.forename: D C
authors.initials: DC
authors.lastname: Wallace
authors.forename: G
authors.initials: G
authors.lastname: Stepien
mesh_terms: D018249:Adenoma, Oxyphilic / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002277:Carcinoma / Q000378:metabolism*; D002467:Cell Nucleus / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000502:physiology; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000235:genetics
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D000226:Mitochondrial ADP, ATP Translocases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006180:Proton-Translocating ATPases