Effects of benzodiazepines on potassium-induced increase in free cytosolic calcium concentration in astrocytes: interactions with nifedipine and the peripheral-type benzodiazepine antagonist PK 11195.
The benzodiazepines diazepam and midazolam at submicromolar concentrations potentiated the increase in free cytosolic calcium concentration in astrocytes in primary cultures evoked by an elevation of the extracellular potassium concentration ([K+]0), but they had little stimulatory effect at normal [K+]0 and none at maximally elevated [K+]0. Nifedipine, an inhibitor of the L-channel, counteracted both the effect of the elevated [K+]0 as such and the benzodiazepine modulation. PK 11195, an antagonist of the peripheral-type benzodiazepine receptor, counteracted the effect of the benzodiazepines, but had no effect on the increase in free cytosolic calcium evoked by the elevated [K+]0.
Zhao
Z
Z
Department of Pharmacology, University of Saskatchewan, Saskatoon, Canada.
Hertz
L
L
Code
W E
WE
D000818:Animals; D014151:Anti-Anxiety Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003975:Diazepam / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004347:Drug Interactions; D007546:Isoquinolines / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008874:Midazolam / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*
D014151:Anti-Anxiety Agents; D002121:Calcium Channel Blockers; D007546:Isoquinolines; D009543:Nifedipine; D003975:Diazepam; D008874:Midazolam; D011188:Potassium; D002118:Calcium; C037850:PK 11195