title: Interactions between arachidonic acid and metabotropic glutamate receptors in the induction of synaptic potentiation in the rat hippocampal slice.
abstract: Perfusion of neither the metabotropic glutamate receptor agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), nor arachidonic acid caused any long-term enhancement of synaptic transmission in the CA1 region of the rat hippocampal slice. However, co-perfusion of ACPD (50 microM) and arachidonic acid (10 microM) for 5 min induced a rapidly evoked and long-lasting enhancement of synaptic transmission. This enhancement persisted in the presence of D(-)-2-amino-5-phosphonopentanoic acid (40 microM) and is therefore independent of NMDA receptor activation. The potentiation was mimicked by perfusion of the phospholipase A2 activator melittin (10 micrograms/ml) for 5 or 10 min, or exogenous phospholipase A2 (1 microgram/ml) for 5 min, immediately before ACPD application. We propose a role for arachidonic acid in the induction of synaptic potentiation, possibly as a retrograde transmitter substance.
authors.affiliation: Department of Biomedical Sciences, University of Aberdeen, Scotland, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Collins
authors.forename: R C
authors.initials: RC
authors.lastname: Smith
authors.forename: S N
authors.initials: SN
authors.lastname: Davies
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism / Q000502:physiology*; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation; D018756:GABA Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008555:Melitten / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism; D054467:Phospholipases A2; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: D016718:Arachidonic Acid; D003515:Cycloleucine; D018756:GABA Antagonists; D008555:Melitten; D010741:Phospholipases A; D054467:Phospholipases A2; D010852:Picrotoxin; D018094:Receptors, Metabotropic Glutamate; C036361:1-amino-1,3-dicarboxycyclopentane