title: Effects of refeeding of undernourished and insulin treatment of diabetic neonatal rats on IGF and IGFBP.
abstract: The effect of refeeding and insulin treatment of undernourished and diabetic neonatal rats, respectively, on the regulation of insulin-like growth factor (IGF) and insulin-like growth factor binding protein (IGFBP) was investigated. The changes in body weight, insulinemia, glycemia, serum IGF-I, and growth hormone (GH) as well as the increase of the 30-kDa IGFBP in undernourished and diabetic neonatal rats previously shown elsewhere were reversed by refeeding and insulin treatment, respectively. Also, changes in liver mRNA expression of IGF-I and-II and IGFBP-1 and -2 were restored in refed undernourished and IGF-I and IGFBP-1 levels recovered in insulin-treated diabetic rats. However, serum GH was still below normal after rehabilitation in both situations. Thus the present results support the idea of a GH-independent IGF/ IGFBP regulation mediated by a balance of insulin and nutrients as has already been suggested in previous neonatal studies.
authors.affiliation: Instituto de Bioquímica (Centro Mixto Consejo Superior de Investigaciones Cientificas-Universidad Complutense de Madrid), Facultad de Farmacia, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Goya
authors.forename: F
authors.initials: F
authors.lastname: Rivero
authors.forename: M A
authors.initials: MA
authors.lastname: Martín
authors.forename: R
authors.initials: R
authors.lastname: Arahuetes
authors.forename: E R
authors.initials: ER
authors.lastname: Hernández
authors.forename: A M
authors.initials: AM
authors.lastname: Pascual-Leone
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D013006:Growth Hormone / Q000097:blood; D007328:Insulin / Q000097:blood / Q000627:therapeutic use*; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009748:Nutrition Disorders / Q000097:blood / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000097:blood / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013002:Somatomedins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D013002:Somatomedins; D013006:Growth Hormone