title: Polyphasic approach to the classification and identification of Gardnerella vaginalis and unidentified Gardnerella vaginalis-like coryneforms present in bacterial vaginosis.
abstract: A taxonomic study of Gardnerella vaginalis and G. vaginalis-like coryneforms was performed in order to clarify the phylogenetic affiliation of these organisms and to improve future identification. We examined 50 strains by performing whole-cell protein and fatty acid analyses, a 16S rRNA sequence analysis, and an extensive phenotypic characterization analysis. The results of both chemotaxonomic techniques which we used divided the organisms into two main clusters, and the 16S rRNA sequence analysis revealed that the clusters represent different genera, which were easily distinguished by the results of classical phenotypic tests. The cluster I strains were identified as G. vaginalis, which was shown to be a close relative of the genus Bifidobacterium. An improved description of G. vaginalis is presented. The cluster II strains belong to or are closely related to Actinomyces turicensis.
authors.affiliation: Department of Microbiology, University Hospital UIA, Antwerp, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: van Esbroeck
authors.forename: P
authors.initials: P
authors.lastname: Vandamme
authors.forename: E
authors.initials: E
authors.lastname: Falsen
authors.forename: M
authors.initials: M
authors.lastname: Vancanneyt
authors.forename: E
authors.initials: E
authors.lastname: Moore
authors.forename: B
authors.initials: B
authors.lastname: Pot
authors.forename: F
authors.initials: F
authors.lastname: Gavini
authors.forename: K
authors.initials: K
authors.lastname: Kersters
authors.forename: H
authors.initials: H
authors.lastname: Goossens
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006194:Gardnerella vaginalis / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D016585:Vaginosis, Bacterial / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S