Contractile properties and protein isoforms of single fibres from the chicken pectoralis red strip muscle.
1. The contractile properties of single muscle fibres of the red strip region of adult chicken pectoralis major (PM) muscle, some of which are known to express an embryonic isoform of myosin heavy chain (MHC), were determined and compared with the properties of the fast white fibres of the PM and the slow tonic fibres of the anterior latissimus dorsi (ALD) muscle. 2. The red strip fibres could be classified into two groups, fast and slow. The mean velocity of unloaded shortening (Vmax) in fast red strip fibres was approximately half the Vmax of fast white fibres. Vmax of slow red strip fibres was less than 20% of the value for fast red strip fibres and was not different from Vmax of ALD fibres. 3. The tension-generating ability, i.e. the maximal isometric tension/fibre cross-sectional area (P0/CSA), was the same in fast red strip fibres and fast white fibres. P0/CSA was approximately 30% lower in slow red strip fibres compared with fast red strip fibres but was 70% greater in slow red strip fibres compared with ALD fibres. 4. The tension-pCa relation of fast red strip fibres was shifted to lower pCa values, indicating a lower calcium sensitivity compared with fast white fibres, and this difference was associated with a difference in troponin T isoform composition. The tension-pCa relation of slow red strip fibres was not different from that in ALD fibres. 5. The difference in Vmax between fast red strip fibres and fast white fibres was associated with different MHC compositions of these fibres. 6. The myofibrillar protein isoform composition of slow red strip fibres was identical to that of the slow tonic fibres of ALD muscle and these two groups of fibres had very similar contractile properties.
Reiser
P J
PJ
Department of Physiology, School of Medicine, University of Wisconsin, Madison 53706, USA.
Greaser
M L
ML
Moss
R L
RL
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007536:Isomerism; D007537:Isometric Contraction / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology*; D009210:Myofibrils / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism*
D018995:Myosin Heavy Chains; D002118:Calcium