Association of phosphatidylinositol 3 kinase to protein kinase C zeta during interleukin-2 stimulation.
Interleukin-2 induces a serine-phosphorylated phosphatidylinositol 3 kinase activity in the mouse T cell line TS1 alpha beta. Moreover, protein kinase C (PKC) zeta directly or indirectly associates with the phosphatidylinositol 3 kinase and the association appears to be necessary for the serine-phosphorylated phosphatidylinositol 3 kinase activity, since release of zeta PKC by competition of binding with peptides spanning the p110 sequence from amino acids 907 to 925 abolishes the serine-phosphorylated phosphatidylinositol 3 kinase activity. This kinase activity is also blocked when zeta PKC expression is inhibited by antisense oligonucleotide. Inhibition of phosphatidylinositol 3 kinase activity by wortmannin does not abolish zeta PKC association.
Gómez
J
J
Centro Nacional de Biotecnología, Universidad Autónoma, Madrid, Spain.
Martínez
C
C
García
A
A
Rebollo
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D019869:Phosphatidylinositol 3-Kinases; D010768:Phosphoserine / Q000276:immunology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects; D013601:T-Lymphocytes / Q000201:enzymology; D000099221:Protein Kinase C zeta
D007376:Interleukin-2; D019869:Phosphatidylinositol 3-Kinases; D010768:Phosphoserine; D017853:Phosphotransferases (Alcohol Group Acceptor); D011493:Protein Kinase C; D017346:Protein Serine-Threonine Kinases; D000099221:Protein Kinase C zeta