Protein A carrying monosize PMMA microbeads for the removal of HIgG from human plasma.
Protein A-incorporated polymethylmethacrylate (PMMA) microbeads were investigated for specific removal of HIgG from human plasma. The microbeads were prepared by a phase inversion polymerization, and activated by periodate oxidation. Protein A was then incorporated by covalent binding onto these microbeads through hydroxyl groups coming from the stabilizer. The amount of incorporated protein A was controlled by the initial concentrations of protein A in the immobilization medium and pH. The maximum protein A immobilization of 0.615 mg protein A/g PMMA, was observed at a pH of 9.5 corresponding to an initial protein A concentration of 0.1 mg/ml. There was no HIgG adsorption onto the plain PMMA microbeads, while high HIgG adsorptions of up to 32 mg HIgG/g PMMA were achieved with human plasma.
Pişkin
E
E
Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
Ayhan
H
H
Bulmuş
E V
EV
Rad
A Y
AY
Falkenhagen
D
D
Weber
C
C
D000327:Adsorption; D001672:Biocompatible Materials; D005374:Filtration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008768:Methylmethacrylates / Q000378:metabolism*; D010316:Particle Size; D013205:Staphylococcal Protein A / Q000378:metabolism / Q000627:therapeutic use*; D013211:Staphylococcus aureus
D001672:Biocompatible Materials; D007074:Immunoglobulin G; D008768:Methylmethacrylates; D013205:Staphylococcal Protein A