Germline transformation of Drosophila virilis mediated by the transposable element hobo.
A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile GO animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known widespread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.
Lozovskaya
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Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Nurminsky
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Hartl
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Sullivan
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DT
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014170:Transformation, Genetic; D019895:Transposases
D017931:DNA Primers; D004251:DNA Transposable Elements; D018840:HSP70 Heat-Shock Proteins; D004254:DNA Nucleotidyltransferases; D019895:Transposases