title: Intravascular lung macrophages play an essential role in lipid entrapment and the inflammatory tissue reaction seen after long-term lipid-based parenteral nutrition in pigs. An ultrastructural study.
abstract: To detect possible interactions between lipid-based total parenteral nutrition (TPN) substrates and mononuclear phagocytes, ultrastructural in vitro and in vivo studies were carried out on material from pigs. Mononuclear phagocytes isolated from peripheral blood, phagocytosed lipid after incubation with 1 mg/ml Intralipid for 24 h. Similarly, lipid was taken up by intravascular macrophages in the lungs and liver after central venous administration of TPN containing 2.3 g/kg body weight/day of Intralipid for 5-7 weeks. Lipid accumulation was almost exclusively found intravascularly in the lungs and liver, and not in macrophages obtained from bronchoalveolar lavage fluid. A morphometric study of the lung capillaries showed that the macrophages in TPN animals had increased in size and number, and occupied a larger portion of the capillary lumina. The macrophages appeared to be activated, but the endothelial lining was well preserved. Free intravascular lipid droplets had a diameter both in vitro and in vivo of about 0.5 micron, indicating good stability of the emulsion. We suggest that the lipid uptake stimulates the macrophages and thereby plays a role in the lung tissue inflammation seen in response to long-term lipid-based TPN in pigs.
authors.affiliation: Cardiothoracic Unit, National Hospital, University of Oslo, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Aksnes
authors.forename: K
authors.initials: K
authors.lastname: Børsum
authors.forename: H
authors.initials: H
authors.lastname: Rollag
authors.forename: T
authors.initials: T
authors.lastname: Hovig
mesh_terms: D000818:Animals; D050356:Lipid Metabolism; D008055:Lipids / Q000008:administration & dosage; D008099:Liver / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D008168:Lung / Q000098:blood supply* / Q000473:pathology* / Q000648:ultrastructure; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000473:pathology* / Q000648:ultrastructure; D010288:Parenteral Nutrition; D010587:Phagocytosis; D013552:Swine; D013997:Time Factors
chemical_list: D008055:Lipids