title: Identification of matrix metalloproteinases and their inhibitor in the articular disc of the craniomandibular joint of the rabbit.
abstract: Connective tissue cells synthesize and secrete matrix metalloproteinases (MMPs), a family of matrix-degrading enzymes (comprising collagenases, gelatinases and stromelysins), which are capable of degrading all the constituent molecules of connective tissues at physiological pH. This investigation documents the synthesis and distribution of MMPs and their inhibitor TIMP-1 (tissue inhibitor of metalloproteinases-1) in the developing articular disc of the craniomandibular joint of the rabbit using indirect immunofluorescence microscopy. Cells of the disc synthesised all three classes of MMPs as well as TIMP-1 in all regions of the disc at all stages examined. MMPs and TIMP-1 were detected as bright intracellular accumulations probably within Golgi vesicles and as occasional diffuse, matrix-bound deposits. These results suggest that MMP-mediated matrix remodelling is a prominent feature of growth in craniomandibular joint disc.
authors.affiliation: Department of Orthodontics and Paediatric Dentistry, UMDS of Guy's Hospital, London, U.K.
authors.forename: J J
authors.initials: JJ
authors.lastname: Breckon
authors.forename: R M
authors.initials: RM
authors.lastname: Hembry
authors.forename: J J
authors.initials: JJ
authors.lastname: Reynolds
authors.forename: M C
authors.initials: MC
authors.lastname: Meikle
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000201:enzymology* / Q000254:growth & development; D017364:Collagenases / Q000032:analysis / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000201:enzymology; D019084:Fluorescent Antibody Technique, Indirect; D018093:Gelatinases / Q000032:analysis / Q000096:biosynthesis; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D011480:Protease Inhibitors / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits; D013704:Temporomandibular Joint / Q000201:enzymology* / Q000254:growth & development; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D006023:Glycoproteins; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3