Fluorometric determination for micro amounts of albumin and globulin fractions without separation by using alpha, beta, gamma, delta-tetra(4'-carboxyphenyl)porphin.
A simultaneous determination of micro amounts of albumin and globulin fractions by fluorometry with only reagent alpha,beta,gamma,delta-tetra(4'-carboxyphenyl)porphin (TCPP) in the aqueous solution has been developed. Under the experimental conditions (5.6 x 10(-7) mol/liter of TCPP, pH 3.0, and at room temperature), the linear range of this assay was 0.09-12 microgram/ml and the detection limits (3 S/N) were 60 ng/ml for albumin and 90 ng/ml for globulin, respectively. The human serum samples were measured satisfactorily by using this method.
Li
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Department of Chemistry, Peking University, Beijing, People's Republic of China.
Li
K A
KA
Tong
S Y
SY
D016477:Artifacts; D005916:Globulins / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D011166:Porphyrins / Q000737:chemistry*; D012709:Serum Albumin / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence
D005916:Globulins; D011166:Porphyrins; D012709:Serum Albumin; C018395:tetracarboxyphenylporphine; D012967:Sodium Dodecyl Sulfate