Production and characterization of monoclonal antibodies against the ribosome-inactivating protein PAP from Phytolacca americana.
Monoclonal antibodies specific to pokeweed antiviral protein (PAP), a ribosome-inactivating protein (RIP), were obtained after six unsuccessful fusions. A special procedure including injections of low doses of purified PAP from spring leaves in a short period was adopted. Some clones highly specific to PAP react with recombinant PAP. One clone cross-reacts with PAP-S isolated from seeds but none cross-reacts with the isoform PAP II isolated from summer leaves. These antibodies represent a useful tool to investigate the mechanisms of PAP biosynthesis and plant protection involving RIPs.
Desvoyes
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Laboratoire de Biochimie et Biologie Moléculaire, U.F.R. des Sciences et Techniques, Université de Franche Comté, Besançon, France.
Dulieu
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Poyet
J L
JL
Adami
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P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D000918:Antibody Specificity; D006825:Hybridomas / Q000737:chemistry / Q000378:metabolism; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification; D011500:Protein Synthesis Inhibitors / Q000276:immunology* / Q000302:isolation & purification; D054789:Ribosome Inactivating Proteins, Type 1
D000911:Antibodies, Monoclonal; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein