A rat model for hypoxic-ischemic brain damage in very premature infants.
We proposed that hypoxic-ischemic injury suffered on postnatal day 1 (P1) in the newborn rat would yield significant and reproducible brain injury simulating that seen in a midgestation human brain. The P1 pup was subjected to ipsilateral common carotid ligation followed by 3.5 h of 6% O2 and demonstrated consistent histological changes in the ipsilateral hemisphere. At P2 neuronal loss and edema were pronounced in cortex and caudate; edema subsequently declined by P3. Astrocytic and microglial reactivity were seen as early as 24 h after injury, and by P10 both were extensive in damaged areas. There was marked loss of white matter on the affected side. We have demonstrated a reproducible model of hypoxic-ischemic injury to the very immature brain that can be used to elucidate mechanisms of neuronal loss and periventricular leukomalacia.
Sheldon
R A
RA
Department of Neurology, University of California San Francisco 94143-0114, USA.
Chuai
J
J
Ferriero
D M
DM
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000473:pathology; D001927:Brain Diseases / Q000209:etiology*; D001929:Brain Edema; D002545:Brain Ischemia / Q000150:complications*; D002339:Carotid Arteries / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D005865:Gestational Age; D006801:Humans; D002534:Hypoxia, Brain / Q000150:complications*; D007231:Infant, Newborn; D007234:Infant, Premature; D008026:Ligation; D017628:Microglia / Q000473:pathology / Q000502:physiology; D009474:Neurons / Q000473:pathology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley