title: Expression of TNF-alpha in pig fetal cells stimulated in vitro.
abstract: Macrophages and lymphocytes of pig fetuses stimulated in vitro with bacterial mitogens such as lipopolysaccharide and Nocardia opaca delipidated cell mitogen showed a high TNF-alpha cytoplasmic expression. TNF-alpha was detected by immunofluorescence in peripheral blood lymphocytes and lymphocytes from the thymic region as early as at 34 d of gestation. Macrophages were the main producers of TNF-alpha at later developmental stages.
authors.affiliation: Department of Immunology and Gnotobiology, Nový Hrádek, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Trebichavský
authors.forename: I
authors.initials: I
authors.lastname: Splíchal
authors.forename: R
authors.initials: R
authors.lastname: Barot-Ciorbaru
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000276:immunology; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D005865:Gestational Age; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008934:Mitogens / Q000276:immunology*; D009615:Nocardia / Q000737:chemistry / Q000276:immunology; D009928:Organ Specificity; D009930:Organic Chemicals; D013552:Swine; D013556:Swine, Miniature / Q000196:embryology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; D008934:Mitogens; C101327:Nocardia delipidated cell mitogen; D009930:Organic Chemicals; D014409:Tumor Necrosis Factor-alpha