title: A modified method for quantitative measurements of cholinergic and adrenergic sialogogue-induced salivation in mice.
abstract: A modified procedure based on that reported by Richter is described for the quantitative measurement of salivation induced by sialogogues in mice. As a new immobilizing technique for mice, the present method uses a combination of light anesthesia produced by a low dose (1.0 g/kg, i.p.) of urethane and a fixing plate, instead of deep anesthesia induced by a high dose (1.8 g/kg, i.p.) of urethane. Unlike Richter's method, the immobilizing technique described here did not potentiate the toxicity of sialogogues and hence no mice died within 48 h after the end of the experiment. This method could easily measure salivation induced by cholinergic agonists (pilocarpine and bethanechol) and adrenergic agonists (phenylephrine and isoproterenol), dose-dependently. Thus, the present method may be useful for studying salivation responses induced by various types of sialogogues under less toxic experimental conditions in mice.
authors.affiliation: Department of Pharmacology, School of Dentistry, Iwate Medical University, Morioka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Murai
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D004305:Dose-Response Relationship, Drug; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010656:Phenylephrine / Q000494:pharmacology*; D010862:Pilocarpine / Q000494:pharmacology*; D012472:Salivation / Q000187:drug effects*
chemical_list: D010862:Pilocarpine; D010656:Phenylephrine; D007545:Isoproterenol