title: Oxytocin-mediated recruitment of slowly cycling cross bridges and isometric force in rat myometrium.
abstract: The relationship between cross-bridge cycling rate and isometric stress was investigated in rat myometrium. Stress production by myometrial strips was measured under resting, K+ depolarization, and oxytocin-stimulated conditions. Cross-bridge cycling rates were determined from measurements of maximal unloaded shortening velocity, using the quick-release method. Force redevelopment after the quick release was used as an index of cross-bridge attachment. With maximal K+ stimulation, stress increased with increased cross-bridge cycling (+76%; P < 0.05) and attached cross bridges (+112%; P < 0.05). Addition of oxytocin during K+ stimulation further increased stress (+30%; P < 0.05). With this force component, the cross-bridge cycling rate decreased (-60%; P < 0.05) similar to that under resting conditions. Attached cross-bridges did not increase with this additional stress. The results suggest two distinct mechanisms mediating myometrial contractions. One requires elevated intracellular calcium and rapidly cycling cross bridges. The other mechanism may be independent of calcium and appears to be mediated by slowly cycling cross bridges, supporting greater unit stress.
authors.affiliation: Magee Women's Research Institute, University of Pittsburgh, Pennsylvania 15213, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Ruzycky
authors.forename: B T
authors.initials: BT
authors.lastname: Ameredes
mesh_terms: D000818:Animals; D005260:Female; D007537:Isometric Contraction / Q000187:drug effects*; D009215:Myometrium / Q000187:drug effects* / Q000502:physiology; D018994:Myosin Light Chains / Q000378:metabolism; D009218:Myosins / Q000502:physiology*; D010052:Ovariectomy; D010121:Oxytocin / Q000494:pharmacology*; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018994:Myosin Light Chains; D010121:Oxytocin; D011493:Protein Kinase C; D009218:Myosins; D011188:Potassium