title: Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids.
abstract: The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.
authors.affiliation: Department of Research and Development, Center of Molecular Immunology, Havana, Cuba.
authors.forename: A M
authors.initials: AM
authors.lastname: Vázquez
authors.forename: M
authors.initials: M
authors.lastname: Alfonso
authors.forename: B
authors.initials: B
authors.lastname: Lanne
authors.forename: K A
authors.initials: KA
authors.lastname: Karlsson
authors.forename: A
authors.initials: A
authors.lastname: Carr
authors.forename: O
authors.initials: O
authors.lastname: Barroso
authors.forename: L E
authors.initials: LE
authors.lastname: Fernández
authors.forename: E
authors.initials: E
authors.lastname: Rengifo
authors.forename: M E
authors.initials: ME
authors.lastname: Lanio
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D005732:Gangliosides / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000032:analysis* / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005732:Gangliosides; D006017:Glycolipids; D009438:Neuraminic Acids; D013433:Sulfoglycosphingolipids; C032592:N-glycolylneuraminic acid