A 34-kilodalton polypeptide is associated with 1,3-beta-glucan synthase activity from the fungus Saprolegnia monoica.
Three major polypeptides of 34, 48 and 50 kDa which appear to copurify with 1,3-beta-glucan synthase activity were isolated by glycerol gradient centrifugation of Chaps-solubilized proteins from the fungus Saprolegnia monoica. The antiserum produced against the 34-kDa polypeptide revealed by protein immunoblotting that this polypeptide copurified with 1,3-beta-glucan synthase during enzyme purification. This antiserum adsorbs the enzymatic activity as well as the 48- and 50-kDa polypeptides. These results indicate that the 34-kDa peptide is a component of the multisubunit protein complex involved in 1,3-beta-glucan synthase activity.
Bulone
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Laboratoire de Biologie Cellulaire Fongique (Bât. 405) Centre de Génétique Moléculaire et Cellulaire UMR-CNRS 5534, Villeurbanne, France.
Fèvre
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D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D000946:Antigens, Fungal / Q000737:chemistry / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D005964:Glucosyltransferases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015151:Immunoblotting; D008565:Membrane Proteins; D008970:Molecular Weight; D009868:Oomycetes / Q000737:chemistry* / Q000201:enzymology* / Q000276:immunology; D011233:Precipitin Tests; D011817:Rabbits; D029702:Schizosaccharomyces pombe Proteins
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase