Influence of 12-O-tetradecanoylphorbol 13-acetate on replication of porcine cytomegalovirus in the 19-PFT-F cell line.
Severe cytopathic effects, formation of swollen cells and small syncitia, appeared in 19-PFT-F cell cultures at 2 days when a large amount of the OF1 strain of porcine cytomegalovirus (PCMV) was inoculated and cultured in media containing 5, 10, 30 and 60 ng per ml of 12-O-tetradecanoylphorbol 13-acetate (TPA). Characteristic focal clusters of swollen cells appeared on the 19-PFT-F monolayers 3 to 4 days after inoculation with a small amount of the virus. Cells in the foci contained specific fluorescent antigens in the nucleus and cytoplasm. The infectivity could be determined for 4 days after inoculation by means of measuring focus formation in the presence of medium containing TPA. The virus replicated more rapidly in the medium with, rather than without, TPA.
Kawamura
H
H
Department of Veterinary Microbiology, College of Agriculture, University of Osaka Prefecture, Japan.
Matsuzaki
S
S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology; D003587:Cytomegalovirus / Q000187:drug effects / Q000502:physiology*; D003593:Cytoplasm / Q000821:virology; D015726:Giant Cells; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D013755:Tetradecanoylphorbol Acetate