Organization of the vagal preganglionic neurons innervating the stomach in the Japanese quail (Conturinx japonica) double labeling fluorescent method.
Cytoarchitectural study indicated that the dorsal motor nucleus of the vagal nerve (nX) was formed by two subnuclei in a coronal section. One of them had large and round neurons, and occupied the dorsal part of the nX (Xd). The second one had small and oval neurons, and occupied the ventral part of the nX (Xv). The double labeling fluorescent method was performed to determine the organization of preganglionic neurons in the nX innervating different muscles of the quail stomach. Injections of nuclear yellow (NY) into the proventriculus, and of fast blue (FB) into the gizzard showed that there were no double-labeled neurons in the nX. NY- and FB-labeled neurons were found throughout the nX. After separate injections of NY and FB into both the cranioventral and caudodorsal thick muscles, and the craniodorsal and caudodorsal thin muscles, many NY- and FB-labeled neurons were distributed and intermingled in the middle two thirds of the nX. Considerable numbers of double-labeled neurons were also found at the middle level of the nX. There was no side-predominancy of the population of the preganglionic neurons innervating the quail stomach. These results show that, many neurons of both sides of the nX are separately innervating both the proventriculus and gizzard, and considerable numbers of single neurons of the nX also innervate a pair of thin or thick muscles with collateral axons.
Sugita
S
S
Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan.
Iizuka
Y
Y
Sugahara
K
K
D000578:Amidines; D000818:Animals; D001562:Benzimidazoles; D003370:Coturnix / Q000033:anatomy & histology*; D005260:Female; D005456:Fluorescent Dyes; D005725:Ganglia, Autonomic / Q000033:anatomy & histology; D009130:Muscle, Smooth / Q000294:innervation*; D016065:Myoelectric Complex, Migrating; D009474:Neurons / Q000166:cytology*; D013270:Stomach / Q000294:innervation*; D014630:Vagus Nerve / Q000033:anatomy & histology* / Q000166:cytology; D000096926:Benzenesulfonamides
D000578:Amidines; D001562:Benzimidazoles; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50; C036574:nuclear yellow; D000096926:Benzenesulfonamides