Determination of amphotericin B in human serum by liquid chromatography.
A rapid reversed-phase high-performance liquid chromatographic method with a 30-mm long column is described for assaying amphotericin B in serum. After deproteinization of serum samples with methanol, the supernatant was injected onto a reversed-phase C18 column, using 2.5 mM Na2EDTA-acetonitrile (70:30, v/v) as the mobile phase. Amphotericin B was eluted at 1.5 min. Calibration plot of the peak area against concentration was linear from 0.05 to 25 micrograms/ml (C.V. of 3%). Within-day and day-to-day imprecision (C.V.) ranged between 1.33% and 3.61%. The application was evaluated in 55 serum samples from patients treated with amphotericin B.
Lopez-Galera
R
R
Biochemistry Service, Hospital General Universitario Vall d'Hebron, Barcelona, Spain.
Pou-Clave
L
L
Pascual-Mostaza
C
C
D000666:Amphotericin B / Q000097:blood*; D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D000666:Amphotericin B