title: Purification and analysis of functional preinitiation complexes.
authors.affiliation: Wellcome Sciences Institute, Department of Biochemistry, University of Dundee, United Kingdom.
authors.forename: S G
authors.initials: SG
authors.lastname: Roberts
authors.forename: M R
authors.initials: MR
authors.lastname: Green
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002474:Cell-Free System; D002850:Chromatography, Gel; D004247:DNA / Q000235:genetics; D005057:Eukaryotic Cells / Q000737:chemistry*; D006367:HeLa Cells / Q000737:chemistry; D046911:Macromolecular Substances; D008863:Microspheres; D012319:RNA Polymerase II / Q000302:isolation & purification*; D019809:Streptavidin; D016385:TATA Box; D013698:Templates, Genetic; D035561:Transcription Factor TFIIA; D035581:Transcription Factor TFIIB; D035362:Transcription Factor TFIID; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000302:isolation & purification*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances; D035561:Transcription Factor TFIIA; D035581:Transcription Factor TFIIB; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; D004247:DNA; D019809:Streptavidin; D012319:RNA Polymerase II; C056604:transcription factor TFIIF