title: A non-expressed transgene as a cell marker for the investigation of cellular traffic after splenic autotransplantation.
abstract: The investigation of cellular interactions during the regeneration of splenic transplants depends upon distinguishing host cells from graft derived cells. As a cell marker which, in contrast to many other marker systems, does not affect histocompatibility, the non-expressed transgene mMT-HGHRH-1 was introduced by mating into NMRI inbred mice to generate the transgenic mouse line BSM. By transplanting non-transgenic NMRI splenic tissue into transgenic BSM hosts, host derived cells could be identified in the transplants by in situ hybridization after regeneration. In a reciprocal approach, implant derived cells were detected in a transgenic transplant after 3 weeks of regeneration in a non-transgenic host. Relative amounts of transgenic cells in transplant cross-sections were estimated from the signal intensity of autoradiographs by densitometry and computer analysis. This approach could be broadly applied in studies of transplantation systems.
authors.affiliation: University of Salzburg, Department of Biochemistry, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Hammerl
authors.forename: D
authors.initials: D
authors.lastname: Weinlich
authors.forename: A
authors.initials: A
authors.lastname: Hartl
authors.forename: J
authors.initials: J
authors.lastname: Thalhamer
mesh_terms: D000818:Animals; D002470:Cell Survival; D002843:Chromatin / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D012038:Regeneration / Q000502:physiology*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D019076:Transgenes
chemical_list: D002843:Chromatin; D005819:Genetic Markers