The formation of a crosslinked carboxyhemoglobin membrane at an organic-aqueous interface.
A 30% carboxyhemoglobin solution was encapsulated with a membrane composed of crosslinked hemoglobin as a potential artificial red blood cell by adding a 25 microL droplet of 30% carboxyhemoglobin solution in phosphate buffer to a buffer-saturated toluene solution of a homobifunctional crosslinking agent. Disuccinimidyl glutarate, disuccinimidyl suberate, and terephthaldicarboxaldehyde proved successful in crosslinking hemoglobin at concentrations of 0.05%, 0.08%, and 4.3%, respectively (saturated levels in buffer-saturated toluene). Disuccinimidyl glutarate exhibited maximal crosslinking of 11.0% in approximately 30 minutes. Disuccinimidyl suberate and terephthaldicarboxaldehyde required at least 12 hours to form a viable sphere and crosslinked 39.4% and 37.8% of the hemoglobin in 18 hours. Membrane integrity was assessed by determining the permeability of uncrosslinked hemoglobin through the membrane as a function of time.
Eisman
J M
JM
Department of Pharmaceutics, Philadelphia College of Pharmacy and Science, Pennsylvania 19104-4495, USA.
Schnaare
R L
RL
D002263:Carboxyhemoglobin / Q000008:administration & dosage / Q000737:chemistry*; D003432:Cross-Linking Reagents; D004339:Drug Compounding; D008567:Membranes, Artificial; D008863:Microspheres; D010539:Permeability; D012821:Silanes / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D012821:Silanes; D014867:Water; D002263:Carboxyhemoglobin