Rapid neurite formation in a human cortical neuronal cell line.
The subclone HCN-1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics [G. V. Ronnett et al. (1990) Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly. Science 248, 603-605]. As we and others have shown, HCN-1A cells can be induced to differentiate to a neuronal-like morphology. HCN-1A cells stain positively for neurofilament, neuron-specific enolase and the low-affinity neurotrophin receptors, p75NGFR, but not for myelin basic protein, S-100, or glial fibrillary acidic protein (GFAP). HCN-1A cells also stain positively for gamma-aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN-1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN-1A cells in response to their differentiation neuronal phenotype.
Dunn
K J
KJ
Sealy Center for Molecular Biology, University of Texas Medical Branch, Galveston 77555-0652, USA.
Perez-Polo
J R
JR
Wood
T G
TG
D016475:3T3 Cells / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000166:cytology; D048429:Cell Size / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology*; D003598:Cytoskeletal Proteins / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D006801:Humans; D051379:Mice; D016501:Neurites / Q000737:chemistry / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure*; D010641:Phenotype
D003598:Cytoskeletal Proteins