Partition of oleic acid between the lymph and portal blood in rats having a diverted bile-pancreatic duct.
The present study examines the suggestion that in the absence of adequate bile and pancreatic juice, which support the absorption from the gut of long-chain fatty acids into lymph, the fatty acids are absorbed directly into the portal blood. Oleic acid (18:1) partitioning between lymph and portal blood was investigated in intact and bile- and pancreatic juice-diverted rats. In a first set of experiments, 18:1 absorption from the gut into lymph and blood was studied by continuous recovery of the mesenteric lymph for 6 h and mesenteric portal venous blood for 1 h. In a second set of experiments, esterification processes were investigated by study of the mucosal distribution of labelled lipids and by mono- and diacylglycerol acyltransferase (EC 2.3.1.22 and EC 2.3.1.20 respectively) specific activities. In the bile- and pancreatic juice-diverted rats the absorption of labelled 18:1 into lymph was significantly reduced during the first 3 h of intraluminal infusion of this substrate. In such rats a compensatory absorption of labelled 18:1 into mesenteric portal blood was not observed. At 6 h after micellar lipid-mixture infusion, the overload of lipids both in free form and as triacylglycerols persisting in the mucosa paralleled the lower acyltransferase specific activities observed in bile- and pancreatic juice-diverted rats. These studies demonstrate the absence of a previously proposed compensatory absorption of 18:1 into portal blood when absorption into lymph is impaired by an inadequate supply of bile and pancreatic juice.
Mathieu
Y
Y
Département de Nutrition, Université de Bourgogne, Dijon, France.
Caselli
C
C
Bernard
A
A
Carlier
H
H
D000818:Animals; D001646:Bile / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008102:Liver Circulation; D008196:Lymph / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000097:blood / Q000493:pharmacokinetics*; D010189:Pancreatic Juice / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004075:Diglycerides; D005989:Glycerides; D009829:Oleic Acids