title: Increased liver-specific catalase activity in transgenic mice.
abstract: Catalase is the major peroxisomal H2O2-detoxifying enzyme and is thought to be critical in maintaining low H2O2 levels within a cell. It has been proposed that increased H2O2 levels may be involved in oxidative DNA damage and tumor promotion induced by peroxisome proliferators and other xenobiotics. To develop a mouse model system to address this issue, we have generated transgenic mice that exhibit a three- to four-fold increase in liver catalase levels. The activities of fatty acyl coenzyme A (CoA) oxidase and lauric acid hydroxylase were unchanged in transgenic mice, demonstrating that elevated catalase levels did not alter the activity of these other peroxisome proliferator-induced enzymes that produce active oxygen. These mice should help elucidate the role of H2O2 in cellular events mediated by peroxisome proliferators and other xenobiotics.
authors.affiliation: Graduate Center for Toxicology, University of Kentucky, Lexington 40506, USA.
authors.forename: V
authors.initials: V
authors.lastname: Nilakantan
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: H P
authors.initials: HP
authors.lastname: Glauert
authors.forename: B T
authors.initials: BT
authors.lastname: Spear
mesh_terms: D042962:Acyl-CoA Oxidase; D000818:Animals; D001483:Base Sequence; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004247:DNA / Q000032:analysis; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D005914:Globins / Q000235:genetics; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008822:Mice, Transgenic; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010088:Oxidoreductases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D005914:Globins; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D002374:Catalase; D042926:Cytochrome P-450 CYP4A; D042962:Acyl-CoA Oxidase