title: Effect of a bradykinin antagonist on the local inflammatory response following thermal injury.
abstract: Partial and full thickness burns with intervening zones of stasis were created on the backs on New Zealand White rabbits (n = 23). Either saline or the bradykinin receptor antagonist, NPC 17731, was administered. Skin blood flow was measured hourly using a laser Doppler blood flowmeter. After 4 h skin samples were harvested for assessment of tissue oedema (wet/dry weights) and leucocyte accumulation (immunohistochemistry). Statistical analysis was performed using Analysis of variance (ANOVA) and Mann-Whitney U test with a level of significance at P < 0.05. It was found that blood flow was decreased postburn in all groups. Bradykinin antagonist resulted in increased blood flow in partial thickness burns and zones of stasis compared to saline-treated animals (P < 0.05). Pretreatment with bradykinin antagonist showed reduced tissue oedema in full thickness burns (P < 0.05). No significant difference was observed in leucocyte accumulation between both groups. These data suggest a role for bradykinin in the pathogenesis of postburn microvascular changes which is independent of leucocyte-mediated injury.
authors.affiliation: Department of Surgery, University of Texas, Southwestern Medical Center, Dallas, USA.
authors.forename: F E
authors.initials: FE
authors.lastname: Nwariaku
authors.forename: P J
authors.initials: PJ
authors.lastname: Sikes
authors.forename: E
authors.initials: E
authors.lastname: Lightfoot
authors.forename: W J
authors.initials: WJ
authors.lastname: Mileski
authors.forename: C
authors.initials: C
authors.lastname: Baxter
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D001920:Bradykinin / Q000502:physiology; D065168:Bradykinin Receptor Antagonists; D002056:Burns / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D003872:Dermatitis / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D004487:Edema / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D007262:Infusions, Intravenous; D017078:Laser-Doppler Flowmetry; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D012867:Skin / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology
chemical_list: D065168:Bradykinin Receptor Antagonists; D009842:Oligopeptides; C071327:NPC 17731; D001920:Bradykinin