Commercially available antibodies to human interleukin-1 do not crossreact with porcine interleukin-1.
Interleukin-1 (IL-1) is a key mediator in inflammatory processes. There are several immunoassays for human, but not porcine, IL-1 commercially available. Four of these were tested on porcine specimens. IL-1 was not detected, by any of the immunoassays, in cell culture supernatants with confirmed IL-1 activity in a bioassay. The results indicate that the tested antibodies to human IL-1 do not crossreact with porcine IL-1.
Holst
H
H
Department of Clinical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Magnusson
U
U
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001681:Biological Assay; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D007375:Interleukin-1 / Q000276:immunology*; D051379:Mice; D013552:Swine
D000911:Antibodies, Monoclonal; D007375:Interleukin-1