Random amplified polymorphic DNA markers of the Brassica alboglabra chromosome of a B. campestris-alboglabra addition line.
The alien C-genome chromosome in a Brassica campestris alboglabra monosomic addition line was characterized by random amplified polymorphic DNA (RAPD) analysis. The alien chromosome carried three loci, Ec, Wc and Lap-1C, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine aminopeptidase (Lap-1Cc) respectively. The RAPD analysis revealed 17 markers specific to the alien chromosome. Among 45 offspring plants from the selfed addition line the alien C-chromosome was transmitted to 15 plants, four plants had only parts of this chromosome and the remaining 26 plants did not carry the chromosome.
Jørgensen
R B
RB
Environmental Science and Technology Department, Risø National Laboratory, Roskilde, Denmark.
Chen
B Y
BY
Cheng
B F
BF
Heneen
W K
WK
Simonsen
V
V
D000483:Alleles; D001937:Brassica / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D003582:Cytogenetics; D004587:Electrophoresis, Agar Gel; D004883:Erucic Acids / Q000378:metabolism; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D007931:Leucyl Aminopeptidase / Q000235:genetics / Q000378:metabolism; D008938:Mitosis / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D004883:Erucic Acids; D005819:Genetic Markers; C049811:erucic acid; D007931:Leucyl Aminopeptidase