Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer.
A retroviral vector containing the wild-type p53 gene under control of a beta-actin promoter was produced to mediate transfer of wild-type p53 into human non-small cell lung cancers by direct injection. Nine patients whose conventional treatments failed were entered into the study. No clinically significant vector-related toxic effects were noted up to five months after treatment. In situ hybridization and DNA polymerase chain reaction showed vector-p53 sequences in posttreatment biopsies. Apoptosis (programmed cell death) was more frequent in posttreatment biopsies than in pretreatment biopsies. Tumor regression was noted in three patients, and tumor growth stabilized in three other patients.
Roth
J A
JA
Department of Thoracic and Cardiovascular Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Nguyen
D
D
Lawrence
D D
DD
Kemp
B L
BL
Carrasco
C H
CH
Ferson
D Z
DZ
Hong
W K
WK
Komaki
R
R
Lee
J J
JJ
Nesbitt
J C
JC
Pisters
K M
KM
Putnam
J B
JB
Schea
R
R
Shin
D M
DM
Walsh
G L
GL
Dolormente
M M
MM
Han
C I
CI
Martin
F D
FD
Yen
N
N
Xu
K
K
Stephens
L C
LC
McDonnell
T J
TJ
Mukhopadhyay
T
T
Cai
D
D
D000368:Aged; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000628:therapy*; D017931:DNA Primers; D018014:Gene Transfer Techniques; D016158:Genes, p53; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods; D005822:Genetic Vectors / Q000009:adverse effects / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012190:Retroviridae / Q000235:genetics*
D017931:DNA Primers