The structure and characterization of the surface protein layer of a Comamonas-like organism.
The regular surface layer of a strain of a Comamonas-like organism was examined by electron microscopy. The surface layer protein was easily extracted from the cell surface by a 2.5 M solution of lithium chloride. The protein subunit has a molecular size of 32,000 daltons, but usually forms a large aggregate of more than 1,200,000 daltons. In the extract it formed a regular array of p4 symmetry and was observed to be intimately associated with fragments of lipopolysaccharide. The size of a subunit determined by the negative staining method and the image processing method measured 5.2 x 6.4 nm (width and length), was arranged in a cobblestone-like pattern, and was located in a lattice space measuring 13.0 nm square.
Wai
S N
SN
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Takade
A
A
Fujimoto
S
S
Amako
K
K
D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry* / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D008854:Microscopy, Electron; D015394:Molecular Structure; D008970:Molecular Weight; D011487:Protein Conformation
D001426:Bacterial Proteins; D008565:Membrane Proteins