Amyloid beta protein in rat soleus muscle in chloroquine-induced myopathy using end-specific antibodies for A beta 40 and A beta 42: immunohistochemical evidence for amyloid beta protein.
Previous immunohistochemical studies from this laboratory demonstrated that monoclonal antibodies raised against various regions of amyloid precursor protein (APP) (i.e., N-terminus, amyloid beta protein (A beta), and C-terminus) strongly labeled vacuoles in chloroquine-induced myopathy-affected muscle in rats. In this study, we used antibodies end specific for the A beta 40 and A beta 42 species, and a monoclonal antibody to A beta 1-9 which reacts with APP and A beta. Most vacuoles clearly reacted with anti-A beta 1-9, while about half reacted with anti-A beta 42, and only a few reacted with anti-A beta 40. These results demonstrate that vacuoles in chloroquine-induced myopathy-affected muscle contain cleaved A beta, and that distribution of the two major A beta species is similar to what is observed in A beta deposition in Alzheimer's disease (AD)-affected brain. This provides further evidence that chloroquine-induced myopathy in rats provides a suitable model to understand APP processing into A beta, and the role of APP in terms of the pathogenesis of AD.
Tsuzuki
K
K
Department of Microbiology, Sapporo Medical University, Japan.
Fukatsu
R
R
Takamaru
Y
Y
Yoshida
T
T
Hayashi
Y
Y
Yamaguchi
H
H
Fujii
N
N
Takahata
N
N
D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000276:immunology; D016564:Amyloid beta-Protein Precursor / Q000276:immunology / Q000648:ultrastructure; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000962:Antimalarials / Q000009:adverse effects; D002738:Chloroquine / Q000009:adverse effects; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D000911:Antibodies, Monoclonal; D000962:Antimalarials; D002738:Chloroquine