New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.
Vogel
J U
JU
Institute of Medical Virology, JWG-University Frankfurt, Frankfurt am Main, Germany.
Cinatl
J
J
Lux
A
A
Weber
B
B
Driesel
A J
AJ
Doerr
H W
HW
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis / Q000821:virology; D001483:Base Sequence; D002493:Central Nervous System Diseases / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012015:Reference Standards; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral