Chicken gizzard calcyclin--distribution and potential target proteins.
The distribution of calcyclin in some chicken tissues was studied by Western blotting using polyclonal antibodies raised against calcyclin purified from chicken gizzard. The protein was found in gizzard muscle and in a lesser amount in skeletal and cardiac muscle. No immunological reaction was observed in chicken liver. Immunohistochemical studies of chicken gizzard tissue revealed the presence of calcyclin only in muscle fibers. Ca(2+)-dependent interaction of chicken gizzard calcyclin with potential protein targets was also examined. By gel overlay method it was found that calcyclin bound to three proteins with molecular masses of approximately 35 kDa, 25 kDa and 15 kDa present in the cytosolic fraction derived from chicken gizzard muscle. The chicken gizzard calcyclin was also shown to interact with lysozyme.
Filipek
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A
Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland. anfil@nencki.gov.pl
Wojda
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U
D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002417:Cattle; D018797:Cell Cycle Proteins; D002645:Chickens; D004815:Epidermal Growth Factor; D005895:Gizzard, Avian; D007150:Immunohistochemistry; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry; D009130:Muscle, Smooth / Q000166:cytology*; D009206:Myocardium / Q000737:chemistry; D009928:Organ Specificity; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins
D000906:Antibodies; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489133:S100A6 protein, Gallus gallus; D004815:Epidermal Growth Factor