Grb10: A new substrate of the insulin-like growth factor I receptor.
Using the yeast two-hybrid system, we have isolated a new substrate of the insulin-like growth factor I receptor (IGF-IR), identified as Grb10, a member of the family of SH2 domain proteins. With the help of several mutants of the IGF-IR, we have mapped the binding site of Grb10 between amino acids 1229 and 1245 of the receptor, a sequence that is dispensable for the mitogenic activity of the IGF-IR. Grb10 coprecipitates with the IGF-IR in cell lysates and is probably involved in the regulation of its activity.
Morrione
A
A
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Valentinis
B
B
Li
S
S
Ooi
J Y
JY
Margolis
B
B
Baserga
R
R
D001665:Binding Sites; D051383:GRB10 Adaptor Protein; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000378:metabolism*; D018909:src Homology Domains
D011506:Proteins; D017527:Receptor, IGF Type 2; D051383:GRB10 Adaptor Protein