In vitro study of the catecholaminergic metabolism of locus coeruleus neurones by differential normal pulse voltammetry.
The aim of the present work was to measure, by voltammetry, the catecholaminergic metabolic activity of rat locus coeruleus (LC) neurones in brain slices. For this new experimental approach, we used an optimized protocol of slice preparation intended to prevent neuronal damages due to brain ischaemia. Our results show that the LC neurones exhibit in vitro a stable spontaneous catecholaminergic metabolic activity and that, as in vivo, 3,4-dihydroxyphenylacetic acid (DOPAC) is likely to be the main contributor to the recorded signal. This catecholaminergic metabolic activity can be pharmacologically altered by administering carbachol and clonidine to the superfusion fluid. We also determined the values of bath temperature and superfusion flow rate providing, in our methodological conditions, an optimal catecholaminergic metabolic activity. Finally, we took advantage of both the direct accessibility to the LC and the compactness of this nucleus to determine the spatial resolution of differential normal pulse voltammetry. In conclusion, the study of the subregional mechanisms controling the catecholaminergic metabolism in LC neurones can be performed in brain slices by differential normal pulse voltammetry.
Vachette
C
C
Laboratoire de Neuropharmacologie, Université Claude Bernard and CNRS UMR 105, Lyon, France.
Nowak
L G
LG
Amouretti
X
X
Renaud
B
B
Bullier
J
J
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002478:Cells, Cultured / Q000502:physiology; D004594:Electrophysiology / Q000379:methods*; D005110:Extracellular Space / Q000737:chemistry; D008125:Locus Coeruleus / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D010507:Periodicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature
D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid