title: Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems.
abstract: We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding approach using conventional recombinant DNA methodologies and two-component ligations failed to give the desired product.
authors.affiliation: Salem-Teikyo University/Tampa Bay Research Institute, St. Petersburg, FL 33716, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zeng
authors.forename: B R
authors.initials: BR
authors.lastname: Huang
authors.forename: J N
authors.initials: JN
authors.lastname: Lapeyre
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000235:genetics; D005069:Evaluation Studies as Topic; D005822:Genetic Vectors
chemical_list: D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes