Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes.
Two Aspergillus nidulans genes, xlnA and xlnB, encoding the X22 and X24 xylanases from this fungus, respectively, have been cloned and sequenced. Their cDNAs have been expressed in a laboratory Saccharomyces cerevisiae strain under the control of a constitutive yeast promoter, resulting in the construction of recombinant xylanolytic yeast strains.
Pérez-Gonzalez
J A
JA
Departmento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos, Burjassot, Valencia, Spain.
De Graaff
L H
LH
Visser
J
J
Ramón
D
D
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics*
D018076:DNA, Complementary; D004271:DNA, Fungal; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase