title: Proliferation of gemistocytic cells and glial fibrillary acidic protein (GFAP)-positive oligodendroglial cells in gliomas: a MIB-1/GFAP double labeling study.
abstract: Large gemistocytic cells are well-known elements of glial tumors. Recently, miniature gemistocytic cells and neoplastic glial fibrillary acidic protein (GFAP)-positive oligodendroglial cells, which are regularly seen in oligodendrogliomas, have been termed "transitional cells". The proliferative activity of the gemistocytic cell types and the GFAP-positive (gliofibrillary) oligodendrocytes was determined in eight astrocytomas, seven gemistocytic astrocytomas, eight glioblastomas, two monstrocellular glioblastomas, seven oligodendrogliomas and three mixed oligo-astrocytomas by immunohistochemical staining of the proliferation marker MIB-1 in combination with immunostaining for GFAP. Both large gemistocytic cells and the transitional cells showed cytoplasmic GFAP-positive staining. Neither in the classic gemistocytes nor in the minigemistocytes nuclear immunostaining for the MIB-1 antibody was observed. In contrast, MIB-1 staining was seen in the gliofibrillary oligodendrocytes. It is concluded that both large and miniature gemistocytic cell types contrast with gliofibrillary oligodendrocytes by their inability to proliferate.
authors.affiliation: Department of Pathology, University Hospital Rotterdam-Dijkzigt, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Kros
authors.forename: W C
authors.initials: WC
authors.lastname: Schouten
authors.forename: P J
authors.initials: PJ
authors.lastname: Janssen
authors.forename: T H
authors.initials: TH
authors.lastname: van der Kwast
mesh_terms: D034961:Antigens, Nuclear; D001254:Astrocytoma / Q000737:chemistry / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001932:Brain Neoplasms / Q000737:chemistry / Q000473:pathology*; D002455:Cell Division; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D005910:Glioma / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000032:analysis*; D009836:Oligodendroglia / Q000737:chemistry / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D005904:Glial Fibrillary Acidic Protein; D019394:Ki-67 Antigen; D009687:Nuclear Proteins