In vitro cultivation of Angiostrongylus costaricensis eggs to first stage larvae in chemically defined medium.
Angiostrongylus costaricensis eggs were successfully cultured to first stage larvae in a chemically defined medium. The most suitable medium for development was Ham's F-12 among 7 chemically defined media and 10 serum supplemented media examined. The addition of serum to Ham's F-12 did not provide any further benefits for egg development. When the eggs were cultured in this medium under 8% CO2 in air, they developed and formed larvae inside the eggs 5 days later. Thereafter, the eggs began to hatch to first stage larvae. Ten days after cultivation, 34% of the eggs had developed to first stage larvae. When these first stage larvae were infected to the snail intermediate host, Biomphalaria glabrata, they developed to third stage larvae.
Hata
H
H
Department of Parasitology, Chiba University School of Medicine, Japan.
D000801:Angiostrongylus / Q000254:growth & development* / Q000648:ultrastructure; D000818:Animals; D001700:Biomphalaria / Q000469:parasitology; D003470:Culture Media; D005260:Female; D007814:Larva / Q000254:growth & development / Q000648:ultrastructure; D010063:Ovum / Q000254:growth & development / Q000648:ultrastructure; D051381:Rats; D016205:Sigmodontinae
D003470:Culture Media