[The utilization of [9,10-(3)H] stearic and [1-(14)C] linolenic acids in the in-vitro synthesis of lipids in ruminant tissues].
The radioactivity of the synthesized total lipids after in vitro incubation of liver, skeletal muscle and adipose tissue homogenates from ruminants with [9,10-3H]stearic acid was 3.5, 2.9 and 6.6 times higher than after the incubation of these organs and tissues with [1-14C]linolenic acid, respectively compared with the radioactivity of non-esterificant fatty acids fraction being in this case 2.0, 1.7 and 1.6 times lower, respectively. The radioactivity of phospholipids, di- and triglycerides, free and esters cholesterols after the incubation of liver, skeletal muscle and adipose tissue homogenates with [9,10-3H]stearic acid comprised 30.6-33.5, 13.2-18.0, 23.2-32.5, 18.2-23.0, 2.8-3.3 per cent of total lipid radioactivity, respectively, and after the incubation with [1-14C]linolenic acid this correlation was as follows: 46.5-51.5,17.8-33.3, 4.0-10.6, 9.2-15.4 and 0.6-4.5 per cent of total lipids radioactivity.
Zakhariv
O Ia
OIa
Ianovich
V G
VG
D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D012418:Ruminants / Q000378:metabolism*; D012588:Scintillation Counting; D013229:Stearic Acids / Q000378:metabolism*; D014316:Tritium; D017962:alpha-Linolenic Acid / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008055:Lipids; D013229:Stearic Acids; D017962:alpha-Linolenic Acid; D014316:Tritium; C031183:stearic acid
Ispol'zovanie [9,10-(3)H] stearinovoĭ i [1-(14)C] linolenovoĭ kislot v sinteze lipidov in vitro v tkaniakh zhvachnykh.