title: Isolation of nitric oxide synthase in the rat uterus.
abstract: Nitric oxide synthase (NOS) was isolated from the uterus of adult female rats by diethylaminoethyl (DEAE) column and further purified by 2',5'-ADP agarose. The chromatographic properties revealed two isoenzymes, NOS1 and NOS2. The molecular weights of both isoenzymes was approximately 155 Kd by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) which was similar to NOS1 and NOS2 from rat brain. The enzymes required nicotinamide adenine dinucleotide phosphate (NADPH), Ca+2 and calmodulin as cofactors. However, in the absence of calmodulin and/or calcium NOS1 was reduced by approximately 96%, while NOS2 was reduced by approximately 70%. This maximal enzyme activity was similar for brain. These results demonstrate that two isoforms of NOS are present in the rat uterus.
authors.affiliation: Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Jaing
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: M S
authors.initials: MS
authors.lastname: Kannan
authors.forename: D E
authors.initials: DE
authors.lastname: Johnson
authors.forename: R L
authors.initials: RL
authors.lastname: Shew
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D005260:Female; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000201:enzymology*
chemical_list: D019001:Nitric Oxide Synthase