Binding of gliadin to lymphoblastoid, myeloid and epithelial cell lines.
The aim of our work was to investigate the in vitro reactivity of gliadin peptides of natural and synthetic origin with various cell lines. We have found that all tested cell lines of human, mouse and rat origin were agglutinated by enzymically digested gliadin (peptic-tryptic- and peptic-tryptic pancreatic digest of alpha-gliadin) in a concentration dependent manner. In order to test the specificity of binding, inhibition studies were performed using a panel of sugars as well as natural and synthetic peptides derived from gliadin. We have found that among twelve tested sugars only fetuin and phosphomannan were able to inhibit the agglutination of K562 cells with peptic-tryptic- but not with peptic-tryptic pancreatic digest of alpha-gliadin. The lack of inhibition by gliadin peptides and most of the saccharides suggests that agglutinating activity of gliadin is the result of a nonspecific binding of gliadin to the cell membrane.
Farré Castany
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MA
Department of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Kocna
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P
Tlaskalová-Hogenová
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D000230:Adenocarcinoma / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D002051:Burkitt Lymphoma / Q000473:pathology; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion; D003110:Colonic Neoplasms / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D054278:Embryonal Carcinoma Stem Cells; D005903:Gliadin / Q000378:metabolism*; D006801:Humans; D007739:L Cells; D037102:Lectins; D015471:Leukemia, Basophilic, Acute / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism*; D008223:Lymphoma / Q000473:pathology; D008264:Macrophages / Q000378:metabolism*; D008351:Mannans / Q000494:pharmacology; D008441:Maxillary Neoplasms / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018243:Teratocarcinoma / Q000473:pathology; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000494:pharmacology
D002241:Carbohydrates; D037102:Lectins; D008351:Mannans; D010455:Peptides; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000509:alpha-Fetoproteins; C032193:glycoprotein receptor GPIb-IX; D005903:Gliadin; C005437:phosphomannan