Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.
We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.
Yamagishi
A
A
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Japan.
Tanimoto
T
T
Suzuki
T
T
Oshima
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D011743:Pyrimidines / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism*
D004269:DNA, Bacterial; D005819:Genetic Markers; D011743:Pyrimidines; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase