Cloning and characterization of murine endothelial constitutive nitric oxide synthase.
Complementary DNA clones encoding mouse endothelial constitutive nitric oxide synthase (ecNOS) were isolated by plaque hybridization from a murine fetal cardiac lambda ZAP II cDNA expression library using a full-length human ecNOS cDNA as the hybridization probe. DNA sequence analysis indicates a 1202 amino acid protein showing significant sequence identity with human as well as bovine ecNOS.
Gnanapandithen
K
K
Department of Medicine, St. Michael's Hospital, University of Toronto, Canada.
Chen
Z
Z
Kau
C L
CL
Gorczynski
R M
RM
Marsden
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D019001:Nitric Oxide Synthase