title: In vitro binding of type 1-fimbriated Escherichia coli to uroplakins Ia and Ib: relation to urinary tract infections.
abstract: Urinary tract infections, caused mainly by Escherichia coli, are among the most common infectious diseases. Most isolates of the uropathogenic E.coli can express type 1 and P fimbriae containing adhesins that recognize cell receptors. While P fimbriae recognize kidney glycolipid receptors and are involved in peyelonephritis, the urothelial for type 1 fimbriae were not identified. We show that type 1-fimbriated E. coli recognize uroplakins Ia and Ib, two major glycoproteins of urothelial apical plaques. Anchorage of E. coli to urothelial surface via type 1 fimbriae-uroplakin I interactions may play a role in its bladder colonization and eventual ascent through the ureters, against urine flow, to invade the kidneys.
authors.affiliation: Department of Urology, New York University School of Medicine, NY 10016, USA.
authors.forename: X R
authors.initials: XR
authors.lastname: Wu
authors.forename: T T
authors.initials: TT
authors.lastname: Sun
authors.forename: J J
authors.initials: JJ
authors.lastname: Medina
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004847:Epithelial Cells; D004903:Erythrocyte Aggregation; D004926:Escherichia coli / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D000882:Haplorhini; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D059470:Tetraspanins; D001743:Urinary Bladder / Q000166:cytology; D014552:Urinary Tract Infections / Q000378:metabolism / Q000382:microbiology*; D060167:Uroplakin Ia; D060169:Uroplakin Ib
chemical_list: D018829:Adhesins, Bacterial; D008562:Membrane Glycoproteins; D059470:Tetraspanins; C086451:UPK1A protein, human; C086452:UPK1B protein, human; C490123:Upk1a protein, mouse; C490124:Upk1b protein, mouse; D060167:Uroplakin Ia; D060169:Uroplakin Ib