title: Alkali therapy extends the period of survival during hypoxia: studies in rats.
abstract: The goal of this study was to evaluate whether sodium bicarbonate might be a useful form of therapy for hypoxic L-lactic acidosis; our aim was to determine if alkali could extend the time of survival in this setting. Hypoxia was induced in anesthetized, paralyzed, artificially ventilated rats by lowering inspired O2 to 5.5%, an amount sufficient to develop a severe degree of L-lactic acidosis. Measuring arterial blood gases frequently permitted maintenance of a near-constant arterial O2 content. Three groups of hypoxic rats were studied: first, no infusions (n = 10); second, sodium bicarbonate at a rate equal to H+ production in the no-infusion group (n = 12); and third, a control for the Na load in the second group as NaCl (n = 17). Survival was close to twofold longer in the sodium bicarbonate group. Part of this beneficial effect seemed to be increased anaerobic glycolysis, producing ATP along with L-lactic acid. In addition, there was a large decrease in the metabolic demand (consumption of O2) in the 7- to 15-min period in the sodium bicarbonate group. Rats exposed to hypoxia and infused with NaCl for 15 min or alkali for 15, 27, or 40 min were then returned to room air; all survived for the subsequent experimental period of 150 min. We found that there is both a rationale and experimental evidence for giving sodium bicarbonate to prolong survival during hypoxia.
authors.affiliation: Renal Division, St. Michael's Hospital, University of Toronto, Ontario, Canada.
authors.forename: F A
authors.initials: FA
authors.lastname: Halperin
authors.forename: S
authors.initials: S
authors.lastname: Cheema-Dhadli
authors.forename: C B
authors.initials: CB
authors.lastname: Chen
authors.forename: M L
authors.initials: ML
authors.lastname: Halperin
mesh_terms: D000140:Acidosis, Lactic / Q000188:drug therapy* / Q000401:mortality; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000468:Alkalies / Q000627:therapeutic use*; D000693:Anaerobiosis; D000818:Animals; D006019:Glycolysis / Q000187:drug effects; D000860:Hypoxia / Q000188:drug therapy* / Q000401:mortality; D019344:Lactic Acid / Q000096:biosynthesis; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017693:Sodium Bicarbonate / Q000627:therapeutic use*; D016019:Survival Analysis
chemical_list: D000468:Alkalies; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D017693:Sodium Bicarbonate