title: Detection of vertical transmission of human immunodeficiency virus type 1 by a commercial polymerase chain reaction assay.
abstract: In a prospective study, a commercial polymerase chain reaction (PCR) system was compared with a conventional procedure, based on PCR and hybridization with a radio-labeled probe, for the detection of human immunodeficiency virus (HIV) infection in 131 blood samples from 80 children born to HIV-seropositive mothers. Twenty-three of these children were HIV infected. The sensitivity and specificity of the commercial assay as compared with the conventional PCR procedure were 100% and 95.1%, respectively. This commercial method simplifies the performance of the conventional PCR technique and can be used to detect HIV type 1 vertical transmission.
authors.affiliation: Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Bermúdez
authors.forename: P
authors.initials: P
authors.lastname: Moreno
authors.forename: R
authors.initials: R
authors.lastname: Delgado
authors.forename: J T
authors.initials: JT
authors.lastname: Ramos
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Contreras
authors.forename: A R
authors.initials: AR
authors.lastname: Noriega
mesh_terms: D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000302:isolation & purification; D015658:HIV Infections / Q000097:blood / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral