Effects of partially hydrogenated fish oil, partially hydrogenated soybean oil and butter on the susceptibility of low density lipoprotein to oxidative modification in men.
OBJECTIVE
To study the effect of partially hydrogenated fish oil (PHFO-diet), partially hydrogenated soybean oil (PHSO-diet) and butterfat (butter-diet) on the susceptibility of low density lipoprotein (LDL) to in vitro oxidative modification.


METHODS
A strictly controlled, randomized, single-blind dietary study with cross-over design.


METHODS
Thirty-three healthy men aged from 21 to 46 years entered the study; 29 men completed the study.


METHODS
Fat provided approximately 35% of the energy intake in all three test diets, and the content of trans-fatty acids was 8.0, 8.5 and 0.9% of energy in the PHFO-, PHSO- and butter-diets, respectively. The subjects consumed all three test diets each during three weeks, in a single-blind, random order. LDL isolated from the participants given the three different diets was subjected to Cu(2+)-induced oxidation.


RESULTS
No significant differences were seen on either conjugated dienes, lipid peroxides, uptake by macrophages or relative electrophoretic mobility of LDL. Vitamin E level in serum from subjects on the PHFO-diet was significantly higher compared to the two other diets. Furthermore, no significant differences were found in the composition of the LDL particle between the three diet groups.


CONCLUSIONS
Our results indicate that consumption of trans-fatty acids does not alter the susceptibility of LDL to oxidative modification.
Halvorsen
B
B
Institute for Nutrition Research, University of Oslo, Norway.
Almendingen
K
K
Nenseter
M S
MS
Pedersen
J I
JI
Christiansen
E N
EN
D000328:Adult; D002079:Butter; D003300:Copper / Q000378:metabolism; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000494:pharmacology*; D002149:Energy Intake; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D006865:Hydrogenation; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D013024:Soybean Oil / Q000494:pharmacology*; D014810:Vitamin E / Q000097:blood
D004041:Dietary Fats; D005395:Fish Oils; D008077:Lipoproteins, LDL; D014810:Vitamin E; D003300:Copper; D013024:Soybean Oil; D002079:Butter