A marine oligobacterium harboring genes known to be part of aromatic hydrocarbon degradation pathways of soil pseudomonads.
The far-ranging distribution of genes for aromatic hydrocarbon catabolism, predominantly studied in soil pseudomonads, is extended to a marine oligobacterium by finding five homologous sequences in a 5.7-kb chromosomal DNA from a new isolate, Cycloclasticus oligotrophus RB1. RB1 is capable of growth in unamended seawater or mineral salts media supplemented with a variety of aromatic compounds, including toluene, o-, m-, or p-xylenes, as sole carbon sources. The five open reading frames, designated xylM, K, G, C1, and C2, are 57% A+T-rich. XylM is predicted to be an integral membrane protein; XylK and XylG possess glutathione S-transferase (GST) and 2-hydroxy-5methyl-6-oxohexa2,4-dienoate dehydrogenase activities, respectively; XylC1C2 are homologs of the large and small subunits of the iron sulfur protein component of the biphenyl dioxygenase (e.g., BphA1A2).
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Biotechnology Research Institute, National Research Council of Canada, Montréal Quebec, Canada.
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D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006838:Hydrocarbons / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012623:Seawater / Q000382:microbiology*; D012988:Soil Microbiology
D003062:Codon; D004269:DNA, Bacterial; D006838:Hydrocarbons