title: MF59 adjuvant enhances the immunogenicity of influenza vaccine in both young and old mice.
abstract: The responses of young (8 week) and old (18 month) mice to influenza vaccine with and without the potent emulsion adjuvant MF59 were compared. In influenza naive mice, vaccine-specific antibody and T-cell proliferation were significantly lower in the old group compared to the young group. Post-immunization cytokine levels and antibody isotype profiles were different in the old compared to the young mice. The addition of the adjuvant MF59, a submicron oil-in-water emulsion composed of 5% v/v squalene, 0.5% v/v Tween 80 and 0.5% v/v Span 85, significantly increased the immune responses of both the young and old naive mice to the vaccine. The responses of the old mice given adjuvant increased to levels equivalent to those of young mice with vaccine alone. In mice previously infected with influenza virus, similarly depressed immune responses to vaccination were detected in the old mice. While the addition of MF59 to the vaccine had little effect on antibody titres of the previously infected young mice, the adjuvant significantly increased the antibody responses of the previously infected old mice. These results suggest that influenza vaccine combined with MF59 may significantly improve immune responses of elderly humans to influenza vaccination.
authors.affiliation: Chiron Corporation, Emeryville, CA 94608, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Higgins
authors.forename: J R
authors.initials: JR
authors.lastname: Carlson
authors.forename: G
authors.initials: G
authors.lastname: Van Nest
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000375:Aging / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D004357:Drug Synergism; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007252:Influenza Vaccines / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011136:Polysorbates / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D013185:Squalene / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007252:Influenza Vaccines; C089950:MF59 oil emulsion; D011136:Polysorbates; D013185:Squalene