title: Density shift of endocytic vesicles induced by uptake of colloidal gold particles in rat hepatocytes.
abstract: The aim of this study was to demonstrate the influence of endocytosed asialoorosomucoid gold complexes (ASOR-Au10, 10 = diameter of gold particles) on the density of endocytic compartments in rat hepatocytes when fractionated on linear sucrose gradients. We show that by loading hepatocytes with ASOR-Au10 for different time intervals, organelles containing gold particles are denser than organelles containing unconjugated asialoorosomucoid (ASOR). This density shift was observed in endosomes, which were loaded with a short pulse (15 minutes) of ASOR- Au10. Also lysosomes, loaded with ASOR-Au10 for 120 minutes at 37 degrees C, display a similar density shift.
authors.affiliation: Department of Biology, University of Oslo, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Brech
authors.forename: T
authors.initials: T
authors.lastname: Løvdahl
authors.forename: R
authors.initials: R
authors.lastname: Kjeken
authors.forename: N
authors.initials: N
authors.lastname: Roos
authors.forename: T
authors.initials: T
authors.lastname: Berg
mesh_terms: D000818:Animals; D001212:Asialoglycoproteins / Q000493:pharmacokinetics; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D018699:Coated Vesicles / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D017979:Gold Colloid / Q000493:pharmacokinetics*; D008099:Liver / Q000166:cytology; D015388:Organelles / Q000378:metabolism; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D051381:Rats
chemical_list: D001212:Asialoglycoproteins; D017979:Gold Colloid; D009961:Orosomucoid; C017030:asialoorosomucoid