title: Enterobacter cloacae producing a Shiga-like toxin II-related cytotoxin associated with a case of hemolytic-uremic syndrome.
abstract: Two Shiga-like toxin-producing organisms were isolated from the feces of an infant with hemolytic-uremic syndrome by PCR followed by colony blot hybridization. One strain was identified as Escherichia coli OR:H9, while the other was identified as Enterobacter cloacae. Both isolates were highly cytotoxic for Vero cells, and Southern hybridization analysis of chromosomal DNA indicated that both contained a single slt-II-related gene and that these genes were located on similarly sized restriction fragments. Nucleotide sequence analysis indicated that the toxin encoded by the E. cloacae slt-II-related gene was very similar to Shiga-like toxin II variants from E. coli, differing from the most closely related toxin by 3 residues in the A subunit.
authors.affiliation: Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia.
authors.forename: A W
authors.initials: AW
authors.lastname: Paton
authors.forename: J C
authors.initials: JC
authors.lastname: Paton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000096:biosynthesis* / Q000633:toxicity; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D004756:Enterobacteriaceae Infections / Q000150:complications* / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial; D006463:Hemolytic-Uremic Syndrome / Q000209:etiology* / Q000382:microbiology; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D022641:Shiga Toxin 2; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D017931:DNA Primers; D004269:DNA, Bacterial; D022641:Shiga Toxin 2