title: An HPLC assay for the norditerpenoid alkaloid methyllycaconitine, a potent nicotinic acetylcholine receptor antagonist.
abstract: The extremely potent and selective nicotinic acetylcholine receptor antagonist methyllycaconitine, MLA, and related norditerpene alkaloids are finding increasing use as neurochemical probes and as targets for structure-activity relationship studies. In this work, an assay procedure for MLA which utilises ion suppression reverse-phase HPLC with UV absorbance detection at 270 nm is described. The method detected 280 ng MLA on column.
authors.affiliation: School of Pharmacy and Pharmacology, University of Bath, Claverton Down, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Coates
authors.forename: I S
authors.initials: IS
authors.lastname: Blagbrough
authors.forename: T
authors.initials: T
authors.lastname: Lewis
authors.forename: B V
authors.initials: BV
authors.lastname: Potter
authors.forename: M G
authors.initials: MG
authors.lastname: Rowan
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis; D000470:Alkaloids / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D018733:Nicotinic Antagonists / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D018733:Nicotinic Antagonists; C054634:methyllycaconitine; D000157:Aconitine