Isolation by HPLC of the positional isomers of heterogeneous doubly branched cyclomaltohexaose having one alpha-D-galactosyl and one alpha-D-glucosyl side chain and determination of their structures by enzymatic degradation.
Each of the six and five positional isomers of 2n- and 6n-O-alpha-D-galactopyranosyl-6(1)-O-alpha-D-glucopyranosyl-cyclomalt ohexaose (alpha-cyclodextrin, alpha CD; 2n: n = 1-6, 6n: n = 2-6), which were produced from 6-O-alpha-D-glucopyranosyl-alpha CD and melibiose by transgalactosylation with coffee bean alpha-galactosidase, were isolated by high-performance liquid chromatography (HPLC) on a reversed-phase column and a graphitized carbon column. The structures of those isomers were elucidated by analyses of enzymatic degradation products with cyclomaltodextrin glucanotransferase and glucoamylase.
Okada
Y
Y
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Koizumi
K
K
Kitahata
S
S
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D005690:Galactose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D006026:Glycoside Hydrolases; D007536:Isomerism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; D006026:Glycoside Hydrolases; D005947:Glucose; D005690:Galactose; C032613:alpha-cyclodextrin