Hepatocyte nuclear factor 6, a transcription factor that contains a novel type of homeodomain and a single cut domain.
Tissue-specific transcription is regulated in part by cell type-restricted proteins that bind to defined sequences in target genes. The DNA-binding domain of these proteins is often evolutionarily conserved. On this basis, liver-enriched transcription factors were classified into five families. We describe here the mammalian prototype of a sixth family, which we therefore call hepatocyte nuclear factor 6 (HNF-6). It activates the promoter of a gene involved in the control of glucose metabolism. HNF-6 contains two different DNA-binding domains. One of these corresponds to a novel type of homeodomain. The other is homologous to the Drosophila cut domain. A similar bipartite sequence is coded by the genome of Caenorhabditis elegans.
Lemaigre
F P
FP
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Durviaux
S M
SM
Truong
O
O
Lannoy
V J
VJ
Hsuan
J J
JJ
Rousseau
G G
GG
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D051559:Hepatocyte Nuclear Factor 6; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
D000345:Affinity Labels; D051559:Hepatocyte Nuclear Factor 6; D018398:Homeodomain Proteins; C496607:Onecut1 protein, rat; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA; D005947:Glucose