title: A preliminary study of the effects of laser radiation on collagen metabolism in cell culture.
abstract: A low power Ga-As pulse laser was used to stimulate cultured human embryonic fibroblast cells. Energy fluencies varied from 0-1 J/cm2 over a period of 1-4 days. Fibroblast procollagen production was monitored by the synthesis of [3H] hydroxyproline, and DNA replication was assessed by [3H] thymidine incorporation. Following laser treatment, controlled pepsin digestion measured the increase in cell biostimulation. Maximum increase in collagen production and cell biostimulation occurred after 4 episodes of laser treatment at 24-hour intervals. Laser doses between 0.099 and 0.522 J/cm2 had the most significant stimulatory effects on fibroblast function. Clinical efficacy of the low power Ga-As pulse laser may be related to enhanced connective tissue repair.
authors.affiliation: Department of Dentistry, Dental School, University of Queensland.
authors.forename: S M
authors.initials: SM
authors.lastname: Skinner
authors.forename: J P
authors.initials: JP
authors.lastname: Gage
authors.forename: P A
authors.initials: PA
authors.lastname: Wilce
authors.forename: R M
authors.initials: RM
authors.lastname: Shaw
mesh_terms: D001151:Arsenic; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism* / Q000528:radiation effects; D003238:Connective Tissue / Q000378:metabolism / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects; D005708:Gallium; D006801:Humans; D006909:Hydroxyproline / Q000096:biosynthesis / Q000528:radiation effects; D007834:Lasers; D010434:Pepsin A; D011347:Procollagen / Q000096:biosynthesis / Q000378:metabolism / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D014316:Tritium
chemical_list: D011347:Procollagen; D014316:Tritium; D003094:Collagen; D005708:Gallium; D010434:Pepsin A; D001151:Arsenic; D006909:Hydroxyproline; D013936:Thymidine