Cloning and characterization of CpG islands of the human chromosome 1p36 region.
CpG islands were identified and localized to chromosome 1p36 by means of pulsed-field gel blot hybridization with 1p36-specific microclone probes. Five CpG islands, designated CpG17, CpG28, CpG60, CpG112a, and CpG112b, were molecularly cloned from corresponding cosmids. All five islands are associated with transcribed sequences, as shown by RNA blot hybridizations. Screening of cDNA libraries with the island-specific genomic probes led to the isolation of two cDNA clones to date. These encode the human transcription factor E2F-2 and the dominant-negative helix-loop-helix gene ID3, respectively. Pulsed-field gel electrophoresis analysis also revealed that these two genes are located next to each other at a distance of about 25 kb.
Ellmeier
W
W
Research Institute of Molecular Pathology, Vienna, Austria.
Barnas
C
C
Kobrna
A
A
Kleiner
E
E
Kurzbauer
R
R
Weith
A
A
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D018899:CpG Islands; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050703:E2F2 Transcription Factor; D016521:Electrophoresis, Gel, Pulsed-Field; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D006801:Humans; D051796:Inhibitor of Differentiation Proteins; D009363:Neoplasm Proteins; D012313:RNA / Q000235:genetics; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000235:genetics
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D015342:DNA Probes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050703:E2F2 Transcription Factor; C493761:E2F2 protein, human; D051796:Inhibitor of Differentiation Proteins; D009363:Neoplasm Proteins; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors; C075640:ID3 protein, human; D012313:RNA