Chromosome painting in biological dosimetry: assessment of the ability to score stable chromosome aberrations using different pairs of paint probes.
We exposed human peripheral lymphocytes in vitro to 0.3 and 1 Gy of 60Co gamma rays to evaluate whether the ability and sensitivity to detect chromosomal aberrations by chromosome painting is independent or not to the specific paint probes. To detect structural aberrations (translocations), we painted chromosome spreads simultaneously with two whole-chromosome libraries for chromosomes 1, 2, 3, 4, 5, 6, 7, 11, 13, 16, and 18. To compare the rate of chromosome translocations detected by the different pairs of chromosomes, data were normalized according to the fraction of genome painted and evaluated by unconditional logistic regression. Our results show that any combination of paint probes can be used to score induced chromosomal aberrations. We observed that the amounts of translocations are dose dependent and quite homogeneous within each dose of radiation, independently of chromosomes painted. However, the use of small chromosome probes is not recommended because of the high number of cells to be analyzed due to the small amount of genome painted and because it is more difficult to detect translocations in small chromosomes.
García Sagredo
J M
JM
Department of Medical Genetics, University Hospital Ramón y Cajal, Madrid, Spain. jose.m.garcia@hrc.es
Vallcorba
I
I
López-Yarto
Sanchez-Hombre
M D
MD
Resino
M
M
Ferro
M T
MT
D000328:Adult; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes; D015342:DNA Probes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D013194:Staining and Labeling; D014178:Translocation, Genetic / Q000528:radiation effects
D003037:Cobalt Radioisotopes; D015342:DNA Probes