Detection of immobilized amplicons by ELISA-like techniques.
The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.
Oroskar
A A
AA
Nalge Nunc International, Naperville, IL 60563-1796, USA.
Rasmussen
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SE
Rasmussen
H N
HN
Rasmussen
S R
SR
Sullivan
B M
BM
Johansson
A
A
D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D001909:Leukemia Virus, Bovine / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012116:Resins, Plant
D004279:DNA, Viral; D012116:Resins, Plant