A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen.
A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.
House
J A
JA
USDA/APHIS/VS/NVSL Foreign Animal Disease Diagnostic Laboratory Greenport (FADDL), New York 11944, USA.
Stott
J L
JL
Blanchard
M T
MT
LaRocco
M
M
Llewellyn
M E
ME
D000355:African Horse Sickness / Q000175:diagnosis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology / Q000528:radiation effects; D002213:Capsid / Q000276:immunology* / Q000528:radiation effects; D036022:Capsid Proteins; D002417:Cattle; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018397:Equidae; D005720:Gamma Rays; D006736:Horses; D007075:Immunoglobulin M / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D018163:Orbivirus / Q000276:immunology; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007075:Immunoglobulin M; C050779:VP7 protein, Rotavirus