The ovary retains male potential after the thermosensitive period for sex determination in the turtle Emys orbicularis.
Emys orbicularis is a turtle with temperature-dependent sex determination. The thermosensitive period (TSP) lies between embryonic stages 16 and 22. Gonadal differentiation begins during this period involving oestrogens. Treatment with oestrogens during TSP results in the differentiation of ovaries at a male-producing temperature (25 degrees C), whereas treatment with an antioestrogen (tamoxifen) or with nonsteroidal aromatase inhibitors results in gonadal masculinization at a female-producing temperature (30 degrees C). The present study examines the effects on the ovary of inhibiting aromatase activity after TSP. Eggs of E. orbicularis incubated at 30 degrees C were given five or seven applications of 10 micrograms aromatase inhibitor Letrozole (CGS 20267) in ethanol, between stages 22+ and 24-25 when ovarian aromatase activity strongly increases. Individuals which received five applications were sacrificed at stages 24(+)-25. Those which received seven applications were sacrificed either at stage 25+ (close to hatching), or 34-36 days after hatching. Gonadal aromatase activity and related gonadal structure were studied in each individual. In the three series, the gonadal aromatase activity in individuals treated with Letrozole varied from similar or close to that in controls to much lower, and the gonadal structure varied from ovary-like to ovotestis. Ovotestes had the lowest levels of aromatase activity, under 4 fmoles/h/gonad, close to testis levels. They were found in 7 out of 26 individuals given Letrozole. Besides ovotestes, gonads presenting various degrees of masculinization, with enlarged epithelial cords and lacunae in the medulla, were found. Therefore, by inhibiting aromatase activity and thus estrogen synthesis, we were able to obtain the differentiation of testis-like cords or tubes in ovaries of E. orbicularis, after the period of temperature sensitivity. These results show that the ovary retains male potential after this period. Thus, besides their implication during the critical embryonic period for gonadal sex differentiation, oestrogens play a role in maintaining the ovarian structure after this period. A decrease in oestrogen levels could explain some other cases of ovarian masculinization known in vertebrates.
Dorizzi
M
M
Institut Jacques Monod, CNRS, Paris, France.
Richard-Mercier
N
N
Pieau
C
C
D000704:Analysis of Variance; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology; D005260:Female; D000077289:Letrozole; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D010053:Ovary / Q000196:embryology* / Q000201:enzymology; D012732:Sex Determination Analysis; D013696:Temperature; D013737:Testis / Q000196:embryology* / Q000201:enzymology; D014230:Triazoles / Q000494:pharmacology; D014426:Turtles / Q000196:embryology*
D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors; D004967:Estrogens; D009570:Nitriles; D014230:Triazoles; D000077289:Letrozole; D001141:Aromatase