title: Basic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum.
abstract: The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow substrate specificity. Within the external pH range of 5.5 to 7.5, the transmembrane electrical potential (delta psi) functions as the main driving force for uphill transport of arginine, although a low level of uptake was observed when only a transmembrane pH gradient was present. It is concluded that the basic amino acid permease is a H+ symporter. Quantitative analysis of the steady-state levels of arginine uptake in relation to the proton motive force suggests a H+-arginine symport stoichiometry of one to one. Efflux studies demonstrated that the basic amino acid permease functions in a reversible manner.
authors.affiliation: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hillenga
authors.forename: H J
authors.initials: HJ
authors.lastname: Versantvoort
authors.forename: A J
authors.initials: AJ
authors.lastname: Driessen
authors.forename: W N
authors.initials: WN
authors.lastname: Konings
mesh_terms: D026905:Amino Acid Transport Systems; D001120:Arginine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008564:Membrane Potentials; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*; D010806:Physical Education and Training
chemical_list: D026905:Amino Acid Transport Systems; D003574:Cytochrome c Group; D007476:Ionophores; D008081:Liposomes; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D001120:Arginine; D008239:Lysine