title: Endotoxin induces severe inflammatory reactions with necrosis at sites primed with delayed-type hypersensitivity reactions in guinea pigs.
abstract: Guinea pigs immunized with Freund's complete adjuvant received challenge injection of the purified protein derivative of Mycobacterium tuberculosis in the flanks and the corneas to prepare delayed-type hypersensitivity (DTH) reactions. The animals were injected subcutaneously with lipopolysaccharide (LPS) or a synthetic lipid A (LA-15-PP). At the skin site primed with DTH reaction, increased swelling and hemorrhagic reaction followed by a definite necrotic reaction occurred. Severe corneal reactions were also observed in the animals. These findings indicate that bacterial endotoxin modulates DTH reactions and induces severe inflammatory reactions.
authors.affiliation: Department of Microbiology and Immunology, Kagoshima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Kawabata
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: H
authors.initials: H
authors.lastname: Takada
mesh_terms: D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007249:Inflammation / Q000209:etiology* / Q000473:pathology; D008050:Lipid A / Q000633:toxicity; D008070:Lipopolysaccharides / Q000633:toxicity; D009169:Mycobacterium tuberculosis / Q000276:immunology; D009336:Necrosis; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D014373:Tuberculin / Q000633:toxicity
chemical_list: D004731:Endotoxins; D008050:Lipid A; D008070:Lipopolysaccharides; D014373:Tuberculin; D005620:Freund's Adjuvant