Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids.
The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.
Vázquez
A M
AM
Department of Research and Development, Center of Molecular Immunology, Havana, Cuba.
Alfonso
M
M
Lanne
B
B
Karlsson
K A
KA
Carr
A
A
Barroso
O
O
Fernández
L E
LE
Rengifo
E
E
Lanio
M E
ME
Alvarez
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D005732:Gangliosides / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000032:analysis* / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000276:immunology
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D006017:Glycolipids; D009438:Neuraminic Acids; D013433:Sulfoglycosphingolipids; C032592:N-glycolylneuraminic acid