Effects of organic modifiers on the chiral recognition by different types of silica-immobilized bovine serum albumin.
We prepared three columns containing bovine serum albumin immobilized on silica by different means and the effects of organic modifiers in the eluent on chiral separation were studied using N-substituted amino acids. Adsorption on silica, covalent immobilization to diol-silica with carbonyldiimidazole (CSP-II) and covalent immobilization to amino-silica with glutaraldehyde were studied. CSP-II had the highest stereoselectivity and was the most affected by organic modifiers in the eluent. The hydrophobicity of amino acid moiety affected the chiral recognition of N-benzoylamino acids and the aromaticity of the N-substituted group was important.
Harada
K
K
Faculty of Pharmaceutical Sciences, Kumanoto University, Japan.
Yuan
Q
Q
Nakayama
M
M
Sugii
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005976:Glutaral / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007093:Imidazoles; D012710:Serum Albumin, Bovine; C006900:N,N-carbonyldiimidazole; D005976:Glutaral