title: Fertilization promotes selective association of the Abl [correction of AbI] kinase with the egg cytoskeleton.
abstract: Fertilization of the sea urchin egg is known to involve activation of a variety of protein kinases including at least one protein tyrosine kinase. In the present study, fertilization was found to stimulate the association of the Abl [correction of AbI] protein kinase with the detergent-insoluble egg cytoskeleton. Increased levels of Abl protein and of protein tyrosine kinase activity were detected in cytoskeleton preparations made as early as 5 to 15 min after insemination. Immunofluorescence localization demonstrated that the Abl kinase becomes associated primarily with the cortical cytoskeleton of the fertilized egg. A separate, 57 kDa protein tyrosine kinase did not associate with the cytoskeleton indicating that fertilization results in the selective association of the Abl kinase with the cortical cytoskeleton.
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City 66160-7400, USA.
authors.forename: G
authors.initials: G
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Burgess
authors.forename: W H
authors.initials: WH
authors.lastname: Kinsey
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000201:enzymology*; D003902:Detergents; D005306:Fertilization / Q000502:physiology*; D016313:Genes, abl; D010063:Ovum / Q000201:enzymology* / Q000648:ultrastructure; D011505:Protein-Tyrosine Kinases / Q000032:analysis* / Q000235:genetics; D016315:Proto-Oncogene Proteins c-abl / Q000032:analysis*; D012617:Sea Urchins; D012995:Solubility; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D003902:Detergents; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl