[The use of lysolecithin for obtaining a chromosome suspension].
Lysolecithin treatment was used for obtaining isolated metaphase chromosomes from HeLa cells and lymphoblastoid cell lines BOLD and ROM. Chromosomes were stained with Hoechst 33258 and olivomycine. Chromosome quantity in suspension was determined by dual-laser sorter ATC-3000. It is shown that lysolecithin treatment of the cells allows to isolate chromosomes with higher concentration in suspension and less amount of debris in comparison with Triton X-100 treatment. A protocol for chromosome isolation using lysolecithin treatment is suggested.
Shatrova
A N
AN
Aksenov
N D
ND
Zenin
V V
VV
Poletaev
A I
AI
D002458:Cell Fractionation; D002460:Cell Line; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D002877:Chromosomes, Human / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008677:Metaphase; D017830:Octoxynol / Q000494:pharmacology; D013535:Suspensions
D008244:Lysophosphatidylcholines; D013535:Suspensions; D017830:Octoxynol
Primenenie lizoletsitina dlia polucheniia suspenzii khromosom.