Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group.
Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.
Shafer
R W
RW
Stanford University, California, USA.
Winters
M A
MA
Mayers
D L
DL
Japour
A J
AJ
Kuritzkes
D R
DR
Weislow
O S
OS
White
F
F
Erice
A
A
Sannerud
K J
KJ
Iversen
A
A
Pena
F
F
Dimitrov
D
D
Frenkel
L M
LM
Reichelderfer
P S
PS
D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D011088:DNA Ligases; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D007753:Laboratories; D007963:Leukocytes, Mononuclear / Q000821:virology; D009154:Mutation; D010949:Plasma / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D003062:Codon; D017931:DNA Primers; D004279:DNA, Viral; D015215:Zidovudine; D011088:DNA Ligases