Effects of refeeding of undernourished and insulin treatment of diabetic neonatal rats on IGF and IGFBP.
The effect of refeeding and insulin treatment of undernourished and diabetic neonatal rats, respectively, on the regulation of insulin-like growth factor (IGF) and insulin-like growth factor binding protein (IGFBP) was investigated. The changes in body weight, insulinemia, glycemia, serum IGF-I, and growth hormone (GH) as well as the increase of the 30-kDa IGFBP in undernourished and diabetic neonatal rats previously shown elsewhere were reversed by refeeding and insulin treatment, respectively. Also, changes in liver mRNA expression of IGF-I and-II and IGFBP-1 and -2 were restored in refed undernourished and IGF-I and IGFBP-1 levels recovered in insulin-treated diabetic rats. However, serum GH was still below normal after rehabilitation in both situations. Thus the present results support the idea of a GH-independent IGF/ IGFBP regulation mediated by a balance of insulin and nutrients as has already been suggested in previous neonatal studies.
Goya
L
L
Instituto de Bioquímica (Centro Mixto Consejo Superior de Investigaciones Cientificas-Universidad Complutense de Madrid), Facultad de Farmacia, Spain.
Rivero
F
F
Martín
M A
MA
Arahuetes
R
R
Hernández
E R
ER
Pascual-Leone
A M
AM
D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D013006:Growth Hormone / Q000097:blood; D007328:Insulin / Q000097:blood / Q000627:therapeutic use*; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009748:Nutrition Disorders / Q000097:blood / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000097:blood / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013002:Somatomedins / Q000235:genetics / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D013002:Somatomedins; D013006:Growth Hormone