Role of intercellular adhesion molecule-1 in antigen-induced lung inflammation in brown Norway rats.
We investigated the involvement of intercellular adhesion molecule-1 (ICAM-1; CD54) in ovalbumin (OA) antigen-induced lung inflammation in sensitized Brown Norway (BN) rats by using flow cytometry and in vivo treatment with a murine monoclonal antibody (MAb), 1A29, directed against rat ICAM-1. OA-challenge induced an eosinophil and lymphocyte-rich accumulation of leukocytes into the airway lumen. Between 75 and 90% of the T cells in bronchoalveolar lavage (BAL) fluid after challenge expressed CD54 and CD11a and were of the memory phenotype. 1A29 treatment produced dose-related increases in circulating 1A29 and blood neutrophils. In the BAL fluid of 1A29-treated animals, significant (P < 0.05) reductions in the numbers of eosinophils and lymphocytes, but not neutrophils or alveolar macrophages, were observed in association with a reduced inflammatory pathology in lung tissue. 1A29 administration reduced the number of detectable ICAM-1 binding sites on T cells in peripheral blood and BAL fluid examined ex vivo by flow cytometry. We conclude that ICAM-1 is critically important for the antigen-specific recruitment of eosinophils and lymphocytes into the lungs.
Richards
I M
IM
Pharmacia and Upjohn, Inc., Kalamazoo, Michigan 49001, USA.
Kolbasa
K P
KP
Winterrowd
G E
GE
Hatfield
C A
CA
Vonderfecht
S L
SL
Fidler
S F
SF
Griffin
R L
RL
Brashler
J R
JR
Krzesicki
R F
RF
Lane
C L
CL
Anderson
D C
DC
Sly
L M
LM
Staite
N D
ND
Chin
J E
JE
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D001773:Blood Cells / Q000502:physiology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D007114:Immunization; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008221:Lymphoid Tissue / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D010641:Phenotype; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011914:Rats, Inbred BN; D013601:T-Lymphocytes / Q000502:physiology
D000911:Antibodies, Monoclonal; D000941:Antigens; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin