Isolation and chromosomal localization of the human cone cGMP phosphodiesterase gamma cDNA (PDE6H).
By comparing lists of 3'-directed partial cDNA sequences (gene signatures) randomly collected from various tissues, genes unique to each tissue can be identified. A full-size cDNA clone, corresponding to one such tissue-specific gene signature that recurred only in a retina library, was isolated and analyzed. This clone encoded 83 amino acids highly homologous to the bovine blue cone cGMP phosphodiesterase gamma subunit. The retina-specific expression of this gene was confirmed by multiple tissue Northern blotting, and its cone specificity was confirmed by in situ hybridization to a human retina specimen. From these results, we concluded that this clone (HGMW-approved symbol PDE6H) encodes for the gamma subunit of human cone-specific cGMP phosphodiesterase, and it was assigned to chromosome 12p13 by fluorescence in situ hybridization.
Shimizu-Matsumoto
A
A
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Itoh
K
K
Inazawa
J
J
Nishida
K
K
Matsumoto
Y
Y
Kinoshita
S
S
Matsubara
K
K
Okubo
K
K
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015183:Restriction Mapping; D017949:Retinal Cone Photoreceptor Cells / Q000201:enzymology*; D017948:Retinal Rod Photoreceptor Cells / Q000201:enzymology; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D015106:3',5'-Cyclic-GMP Phosphodiesterases; C576560:PDE6H protein, human