Decreased beta 2-adrenergic receptor-mRNA and loricrin-mRNA, and increased involucrin-mRNA transcripts in psoriatic epidermis: analysis by reverse transcription-polymerase chain reaction.
Psoriatic hyperproliferative epidermis is characterized by a decreased beta 2-adrenergic adenylate cyclase response as well as by altered differentiation markers that include decreased loricrin and increased involucrin. Using a quantitative reverse transcription-polymerase chain reaction, we analysed the expression of beta 2-adrenergic receptor-mRNA, loricrin-mRNA, and involucrin-mRNA in the epidermis of five patients with psoriasis vulgaris. The mRNAs of the beta 2-adrenergic receptor and loricrin in the involved epidermis were significantly decreased, by 0.35-fold (P < 0.01) and 0.55-fold (P < 0.05) respectively, compared with uninvolved epidermis. In contrast, the involucrin mRNA expression of the involved epidermis was significantly increased, by 3.77-fold (P < 0.01). No significant difference in beta-actin mRNA transcripts was detected between the involved and the uninvolved epidermis. These results indicate that the altered expression of the beta 2-adrenergic receptor, loricrin, and involucrin, in the psoriatic involved epidermis, is associated with different amounts of each mRNA transcripts.
Takahashi
H
H
Department of Dermatology, Asahikawa Medical College, Japan.
Kinouchi
M
M
Tamura
T
T
Iizuka
H
H
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D001483:Base Sequence; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D008565:Membrane Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; C064554:loricrin; C032253:involucrin