Cyclic (1-->2)-beta-D-glucans excreted by the glucuronan-producing strain Rhizobium meliloti M5N1CS (NCIMB 40472) and by the succinoglycan-producing strain Rhizobium meliloti M5N1.
During fermentation, the mutant strain Rhizobium meliloti M5N1CS, which induces nodule formation on alfalfa roots, produces a partially acetylated (1-->4)-beta-D-glucuronan. In addition to this exopolysaccharide of high molecular weight, the mutant strain produces oligoglucoronates and cyclic (1-->2)-beta-D-glucans with degrees of polymerization from 17 to 30. Under the conditions applied, magnesium has no effect on cyclic glucan production by the mutant strain, but the succinoglycan production by the wild-type strain Rhizobium meliloti M5N1 increases.
Michaud
P
P
Laboratoire de Biotechnologie Microbienne, Université de Picardie Jules Verne, Amiens, France.
Courtois
J
J
Courtois
B
B
Heyraud
A
A
Colin-Morel
P
P
Séguin
J P
JP
Barbotin
J N
JN
D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation; D005936:Glucans / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers; D011134:Polysaccharides / Q000096:biosynthesis*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity; D047071:beta-Glucans
D003470:Culture Media; D005936:Glucans; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; C080135:cyclic beta(1-2)glucan; C088556:mucoric acid; C056779:succinoglycan; D008274:Magnesium