The involvement of PKC in N-cadherin-mediated adherens junction assembly in cultured cardiomyocytes.
Activation of PKC by PMA was shown to promote the separation of chicken cardiomyocytes from each other in culture. Immunofluorescence staining for N-cadherin indicated that PMA, but not its inactive isoform 4 alpha PMA, induces the separation of cardiomyocytes and of co-cultured fibroblasts at intercellular junctional regions. The PMA-induced separation of cardiomyocytes and of co-cultured fibroblasts was inhibited by the PKC inhibitor, H-7. Immunoblot analysis further demonstrated that both PKC iota and PKC lambda were expressed in the cardiomyocyte cultures. While PKC lambda was localized to the cell-cell contact areas between cardiomyocytes, PKC iota was only detectable in the perinuclear cytoplasm of the co-cultured fibroblasts. The present findings suggest the involvement of PKC in regulating N-cadherin-mediated adherens junction formation in chicken cardiomyocytes. The differential distribution of PKC lambda and PKC iota in the cardiomyocytes and in the co-cultured fibroblasts suggests that different PKC isozymes are involved in regulating the assembly of intercellular junctions in these two cell types.
Wu
J C
JC
Department of Anatomy, College of Medicine National Taiwan University, Taipei. jcwu@ntumc1.mc.ntu.edu.tw
Wang
S M
SM
Tseng
Y Z
YZ
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000185:Actinin / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000187:drug effects / Q000378:metabolism*; D007546:Isoquinolines / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015820:Cadherins; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D010879:Piperazines; D000185:Actinin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate