title: Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes.
abstract: Two Aspergillus nidulans genes, xlnA and xlnB, encoding the X22 and X24 xylanases from this fungus, respectively, have been cloned and sequenced. Their cDNAs have been expressed in a laboratory Saccharomyces cerevisiae strain under the control of a constitutive yeast promoter, resulting in the construction of recombinant xylanolytic yeast strains.
authors.affiliation: Departmento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos, Burjassot, Valencia, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Pérez-Gonzalez
authors.forename: L H
authors.initials: LH
authors.lastname: De Graaff
authors.forename: J
authors.initials: J
authors.lastname: Visser
authors.forename: D
authors.initials: D
authors.lastname: Ramón
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase