Significance of nucleosides and a nucleotide mixture infusion on hepatic energy metabolism of 70% hepatectomized rabbits in postoperative phase.
BACKGROUND
The influence of administration of a nucleoside-nucleotide mixture on hepatic energy metabolism was investigated in 70% hepatectomized rabbits from 48 to 72 hours after hepatectomy.


METHODS
From 48 to 72 hours after hepatectomy, animals underwent continuous i.v. infusion of 2 mL/kg body weight/h of 9 g/L NaCl (group S), 5.99 mmol/L nucleoside-nucleotide mixture (group N1) or 11.98 mmol/L nucleoside-nucleotide mixture (group N2).


RESULTS
At 72 hours, there was no significant difference in the hepatic adenylate energy charge among groups (S, 0.84 +/- 0.01; N1, 0.82 +/- 0.02; N2, 0.85 +/- 0.01). The hepatic mitochondrial phosphorylation rate was 66.9 +/- 2.5, 76.3 +/- 11.1, and 108.4 +/- 14.1 nmol ATP/mg mitochondrial protein/min in groups S, N1, and N2, respectively. The value of group N2 was significantly higher than the value of group S (p < .01). In addition, DNA concentration of the remnant liver was 2.27 +/- 0.07, 2.56 +/- 0.17, and 3.19 +/- 0.42 mg/g wet liver in groups S, N1, and N2, respectively, showing a significant increase in group N2 compared with group S (p < .05).


CONCLUSIONS
A continuous i.v. infusion of a nucleoside-nucleotide mixture to 70% hepatectomized rabbits from 48 to 72 hours after hepatectomy prolonged an enhancement of the hepatic mitochondrial phosphorylation rate and elevated DNA content of the remnant liver.
Jin
M B
MB
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Yamaguchi
T
T
Shimahara
Y
Y
Ichimiya
M
M
Kinoshita
K
K
Oka
T
T
Ozawa
K
K
Yamaoka
Y
Y
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D002405:Catheterization, Central Venous; D004247:DNA / Q000032:analysis; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D006498:Hepatectomy; D007262:Infusions, Intravenous / Q000379:methods; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009705:Nucleosides / Q000008:administration & dosage / Q000494:pharmacology*; D009711:Nucleotides / Q000008:administration & dosage / Q000494:pharmacology*; D010766:Phosphorylation; D011184:Postoperative Period; D011817:Rabbits
D000227:Adenine Nucleotides; D009705:Nucleosides; D009711:Nucleotides; D004247:DNA