Conserved regulatory element involved in the early onset of Hoxb6 gene expression.
We have identified a 338 bp DNA fragment, the lateral plate mesoderm (LPM) enhancer, that is highly conserved between mouse and human. The LPM enhancer directs gene expression into the posterior lateral plate mesoderm and hindgut endoderm at early stages of development. By reporter gene analysis in transgenic mice, we demonstrate that both mouse and human DNA sequences possess similar enhancer activity. The expression patterns of the transgene and Hoxb6 during early stages of mouse development are identical, suggesting that the LPM enhancer is involved in the initial activation of Hoxb6 gene expression in posterior regions of mammalian embryos.
Becker
D
D
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Jiang
Z
Z
Knödler
P
P
Deinard
A S
AS
Eid
R
R
Kidd
K K
KK
Shashikant
C S
CS
Ruddle
F H
FH
Schughart
K
K
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D005809:Genes, Regulator; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; C093252:HOXB6 protein, human; D018398:Homeodomain Proteins; C490537:Hoxb6 protein, mouse; D004247:DNA