title: Antisense to the glucocorticoid receptor in hippocampal dentate gyrus reduces immobility in forced swim test.
abstract: Immobility time of rats in the forced swim test was reduced after bilateral infusion of an 18-mer antisense phosphorothioate oligodeoxynucleotide targeted to the glucocorticoid receptor mRNA into the dentate gyrus of the hippocampus. Vehicle-, sense- and scrambled sequence-treated animals spent significantly more time immobile than antisense-treated animals during the initial test. Immunolabeling of the glucocorticoid receptor in brain sections demonstrated a reduced expression of glucocorticoid receptor proteins in antisense-treated dentate gyrus compared to the contralateral sense-treated dentate gyrus or contralateral scrambled sequence-treated dentate gyrus. During the initial test the time spent on immobility was also reduced when rats were treated with the glucocorticoid receptor antagonist RU38486 (17 beta-hydroxy-11 beta-(4-dimethylamino-phenyl)17 alpha-(1-propnyl)estra-4,9-diene-3-one)) 6 h (but not 1 h) earlier. These results demonstrate the participation of glucocorticoid receptors in the expression of immobility in a forced swim test during the initial test.
authors.affiliation: Department of Animal Physiology, University of Groningen, Haren, Netherlands.
authors.forename: S M
authors.initials: SM
authors.lastname: Korte
authors.forename: E R
authors.initials: ER
authors.lastname: De Kloet
authors.forename: B
authors.initials: B
authors.lastname: Buwalda
authors.forename: S D
authors.initials: SD
authors.lastname: Bouman
authors.forename: B
authors.initials: B
authors.lastname: Bohus
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D007267:Injections; D008297:Male; D015735:Mifepristone / Q000008:administration & dosage / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects*; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000235:genetics; D013550:Swimming
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D015735:Mifepristone