title: In vitro effect of interleukin-12 on antigen-specific lymphocyte proliferative responses from persons infected with human immunodeficiency virus type 1.
abstract: The relationship between CD4 lymphocyte count and the in vitro effect of interleukin (IL)-12 on lymphocyte proliferative responses to Candida, tetanus toxoid, and streptokinase antigens was studied in peripheral blood mononuclear cells (PBMC from 30 human immunodeficiency virus (HIV)-infected persons and 10 seronegative controls. IL-12 significantly increased proliferative responses to microbial recall antigens of PBMC from HIV-infected persons with >200 CD4 lymphocytes/mm3 but had little effect on PBMC from patients with more advanced disease. The greatest increase was seen in patients with 200-500 CD4 cells/mm3. Results of limiting dilution analysis suggested that the increase in antigen-specific lymphocyte proliferation in the presence of IL-12 was due to an increase in the number of responding cells rather than an increase in the extent of proliferation of a fixed number of responder cells.
authors.affiliation: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver 80262, USA.
authors.forename: P
authors.initials: P
authors.lastname: Uherova
authors.forename: E
authors.initials: E
authors.lastname: Connick
authors.forename: S
authors.initials: S
authors.lastname: MaWhinney
authors.forename: R
authors.initials: R
authors.lastname: Schlichtemeier
authors.forename: R T
authors.initials: RT
authors.lastname: Schooley
authors.forename: D R
authors.initials: DR
authors.lastname: Kuritzkes
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D018791:CD4 Lymphocyte Count; D002175:Candida / Q000276:immunology; D002478:Cells, Cultured; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000254:growth & development; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013300:Streptokinase / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D013745:Tetanus Toxoid; D018664:Interleukin-12; D013300:Streptokinase