Promoting phorbol ester-TPA enhances migration of C3H 10T1/2 cells. The role of protein kinase C and its relation to carcinogenesis.
Treatment of 10T1/2 cells with promoting phorbol ester drastically enhanced migration of the studied fibroblasts in a serum-supplemented medium. The same cells when exposed to ionomycin or TPA in a serum-free medium did not show any migration. The addition of 1% of serum induced spontaneous and TPA stimulated migration. Also EGF and PDGF separately or together induced the migration of 10T1/2 cells. Parallel studies of protein kinase C documented low enzymatic activity after treatment with TPA, whereas transcripts of PKC were shown independently of TPA treatment.
Janik
P
P
Department of Cell Biology, Maria Skłodowska Cancer Center, Warsaw, Poland.
Szaniawska
B
B
Kowalczyk
D
D
Maternicka
K
K
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D005333:Fetus / Q000187:drug effects / Q000201:enzymology; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000032:analysis / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate