Intravascular infusion of lipid into ewes stimulates production of progesterone and prostaglandin.
Effects of infusion of a lipid emulsion into ewes during mid-to late diestrus on serum concentrations of total cholesterol (TC), progesterone (P4), prostaglandin (PG) F2 alpha metabolite (PGFM), and PGE2, and on ovulation rate were examined. In experiment 1, ewes received infusions of either saline (S, n = 3) or soybean oil emulsion (SB, n = 3) for 5 h on each of Days 9-13 of the estrous cycle. In experiment 2, ewes received infusions of either S (n = 4), SB (n = 5), or olive oil emulsion (OO, n = 5) for 5 h on each of Days 9 through 15 of the estrous cycle. In both experiment 1 and experiment 2, infusion of lipid increased serum concentrations of TC and P4, which declined with time after infusion was terminated (treatment x hour interaction, experiment 1: TC, p < 0.01, P4, p < 0.01; experiment 2: TC, p < 0.01, P4, p < 0.001). Serum PGFM and PGE2 concentrations were greater in lipid-infused ewes than in controls on Days 13 through 15 (treatment x hour interaction; p < 0.03, p < 0.001, respectively). Duration of the estrous cycle was shortened in OO-infused ewes (16.2 +/- 0.4 days) compared with that of SB- and S-infused ewes (17.2 +/- 0.2 and 18.0 +/- 0.0 days, respectively; p < 0.01). Numbers of corpora lutea and follicles, and diameter of follicles > 4 mm did not differ among treatment groups on Day 14 of the succeeding cycle after infusion. These data indicate that lipid infusion stimulated increases in serum concentrations of TC, P4, and prostaglandins and may shorten the estrous cycle.
Burke
J M
JM
Department of Animal Sciences, Oregon State University, Corvallis 97331, USA.
Carroll
D J
DJ
Rowe
K E
KE
Thatcher
W W
WW
Stormshak
F
F
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004031:Diestrus; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D000069463:Olive Oil; D010060:Ovulation / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage; D011374:Progesterone / Q000096:biosynthesis* / Q000097:blood; D011453:Prostaglandins / Q000096:biosynthesis*; D012756:Sheep; D013024:Soybean Oil / Q000008:administration & dosage
D005217:Fat Emulsions, Intravenous; D000069463:Olive Oil; D010938:Plant Oils; D011453:Prostaglandins; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D013024:Soybean Oil; D002784:Cholesterol; D015237:Dinoprost; D015232:Dinoprostone