title: GABAergic stimulation regulates the phenotype of hippocampal interneurons through the regulation of brain-derived neurotrophic factor.
abstract: Gamma-Aminobutyric acid (GABA) switches from enhancing to repressing brain-derived neurotrophic factor (BDNF) mRNA synthesis during the maturation of hippocampal neurons in vitro. Interneurons do not produce BDNF themselves, but BDNF enhances their differentiation. Therefore, the question arose whether hippocampal interneurons regulate their phenotype by regulating BDNF expression and release from adjacent cells. The GABA(A) receptor agonist muscimol and BDNF increased the size and neuropeptide Y (NPY) immunoreactivity of hippocampal interneurons. However, GABAergic stimulation failed to increase NPY immunoreactivity in cultures from BDNF knockout embryos. At later developmental stages, when GABA represses BDNF synthesis, treatment with muscimol induced a decrease in cell size and NPY immunoreactivity of interneurons. Interneurons might thus control their phenotype through the regulation of BDNF synthesis in, and release from, their target neurons.
authors.affiliation: Department of Neurochemistry, Max Planck Institute for Psychiatry, Martinsried, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Marty
authors.forename: B
authors.initials: B
authors.lastname: Berninger
authors.forename: P
authors.initials: P
authors.lastname: Carroll
authors.forename: H
authors.initials: H
authors.lastname: Thoenen
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002452:Cell Count; D002478:Cells, Cultured; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects*; D009118:Muscimol / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D009118:Muscimol; D005680:gamma-Aminobutyric Acid