Reduction of 14-16 kDa allergenic proteins in transgenic rice plants by antisense gene.
An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequence-specific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.
Tada
Y
Y
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Nakase
M
M
Adachi
T
T
Nakamura
R
R
Shimada
H
H
Takahashi
M
M
Fujimura
T
T
Matsuda
T
T
D000485:Allergens / Q000235:genetics / Q000378:metabolism*; D016373:DNA, Antisense / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D012275:Oryza; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D000485:Allergens; D016373:DNA, Antisense; D012333:RNA, Messenger