title: Shear field mapping in actin networks by using magnetic tweezers.
abstract: An improved magnetic bead microrheometer based on phase contrast microscopy allowing high resolution measurements of local deformations within macromolecular network is applied to study local viscoelastic properties of cross-linked actin networks. By embedding nonmagnetic colloidal beads as probes into the networks, the spatial variation of the strain field within cross-linked actin networks can be mapped. Moreover, the Poisson ratio and shear modulus can be measured locally.
authors.affiliation: Physics Department of E22 (Biophysics Group), Technische Universität München, Garching, Germany.
authors.forename: F G
authors.initials: FG
authors.lastname: Schmidt
authors.forename: F
authors.initials: F
authors.lastname: Ziemann
authors.forename: E
authors.initials: E
authors.lastname: Sackmann
mesh_terms: D000199:Actins / Q000737:chemistry*; D003102:Colloids; D004548:Elasticity; D004563:Electrochemistry; D055590:Electromagnetic Phenomena; D005782:Gels; D016012:Poisson Distribution; D012212:Rheology / Q000379:methods*; D014783:Viscosity
chemical_list: D000199:Actins; D003102:Colloids; D005782:Gels