Comparative evaluation of somatic & excretory-secretory antigens of Entamoeba histolytica in serodiagnosis of human amoebiasis by ELISA.
The excretory-secretory antigens collected from the axenic culture medium and conventional somatic antigen prepared from the whole trophozoites of E. histolytica were compared for their efficacy in serodiagnosis of amoebiasis. A total of 280 sera collected from different clinically proven cases of amoebiasis and healthy subjects were analysed against both the antigens in ELISA. Both antigens showed a 100 per cent correlation in amoebic liver abscess cases, patients infected with enteropathogens other than amoeba and healthy subjects. However, excretory-secretory antigens showed slightly higher detection rate in patients suffering from acute amoebic dysentery and asymptomatic cyst passers groups. These results clearly suggested the use of excretory-secretory antigens by ELISA for serodiagnosis of amoebiasis due to its better or equal sensitivity, specificity and easier preparation compare to conventional antigen. The use of excretory-secretory antigen in serodiagnosis will not only help in performing more tests utilizing the same chemicals, but also save the cost, time and troubles for importing the foreign chemicals required for cultivation of E. histolytica.
Pal
S
S
Department of Microbiology, National Institute of Cholera & Enteric Diseases, Calcutta.
Sengupta
K
K
Manna
B
B
Sarkar
S
S
Bhattacharya
S
S
Das
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012698:Serologic Tests
D000953:Antigens, Protozoan