Identification of a 60-kD antigen associated with malignant growth of human breast tissue.
Antisera against a novel 60-kD antigen (P60) were developed using immunomasking strategy which shows malignant growth-related expression in human breast cancer tissue. Cell adhesion assay in the presence of P60 antibody clearly indicates a role for P60 directly or indirectly in adhesion of tumor cells to extracellular matrix protein vitronectin. These results indicate that P60 could be a potential marker to distinguish benign from malignant growth of human breast tissue, and that P60 could be an integrin or a non-integrin receptor, or a receptor-related protein to vitronectin.
Chatterjee
A
A
Department of Biochemistry, Chittaranjan National Cancer Institute, Calcutta, India.
Biswas
N
N
Choudhuri
S K
SK
Mazumder
J
J
Chowdhury
J R
JR
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000502:physiology; D000936:Antigen-Antibody Complex / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D005453:Fluorescence; D006801:Humans; D005779:Immunodiffusion; D007150:Immunohistochemistry; D011817:Rabbits; D019096:Vitronectin / Q000378:metabolism
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D019096:Vitronectin