title: Membrane characterization of amastigote-like forms of Leishmania donovani.
abstract: During in vitro transformation, Leishmania donovani promastigotes converted into amastigote-like forms and underwent several changes in membrane parameters. They exhibited significantly increased microviscosity comparable to true amastigotes. Activities of several functionally important membrane bound enzymes were also altered, thereby indicating a change in their orientation. Peanut agglutinin was found to be specific for agglutination of stationary phase promastigotes whereas wheat-germ agglutinin was specific for the amastigote-like forms as well as for pure amastigotes, implying the presence of specific glycoconjugates on the parasite surface.
authors.affiliation: Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
authors.forename: N
authors.initials: N
authors.lastname: Gupta
authors.forename: N
authors.initials: N
authors.lastname: Goyal
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: A K
authors.initials: AK
authors.lastname: Agrawal
authors.forename: P K
authors.initials: PK
authors.lastname: Seth
authors.forename: A K
authors.initials: AK
authors.lastname: Rastogi
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D006224:Cricetinae; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D007893:Leishmania donovani / Q000254:growth & development* / Q000502:physiology; D008647:Mesocricetus; D008854:Microscopy, Electron
chemical_list: D037102:Lectins