Capillary electrophoresis of glycosylated proteins performed on a conventional capillary gas chromatographic column.
In this paper results are reported which document the suitability of a conventional poly(ethylene glycol) internally coated gas chromatographic capillary column for the capillary electrophoretic analysis of glycoproteins. Although the observed migration times were not in the order of a few minutes and the efficiencies resulted were generally lower than 100000 theoretical plates, the proposed method provides an easy tool for obtaining rapid and reproducible glycosylation profiles of various glycoproteins. The method applies very well even to complicated samples such as, for instance, heavily glycosylated/high-molecular-mass proteins. For the sake of simplicity, reference standard glycoproteins were used as samples.
Bonfichi
R
R
Lepetit Research Center, Gerenzano, Italy.
D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D009961:Orosomucoid / Q000032:analysis; D012259:Ribonuclease, Pancreatic / Q000032:analysis; D012260:Ribonucleases / Q000032:analysis
D006023:Glycoproteins; D009961:Orosomucoid; D012260:Ribonucleases; C029600:ribonuclease B; D012259:Ribonuclease, Pancreatic