title: Genetic tools in pathogenic nocardioform actinomycetes.
abstract: Nocardioform actinomycetes are Gram-positive bacteria with high G+C content. Several species of Mycobacterium, Corynebacterium, Nocardia and Rhodococcus are important human or animal pathogens. Transposon mutagenesis and homologous recombination are powerful genetic tools used for the identification of pathogenicity determinants. Transposable elements with potential application to mutagenize pathogenic nocardioform actinomycetes are described. Homologous recombination experiments which have been recently achieved in mycobacteria and related actinomycetes are commented on.
authors.affiliation: Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, España. navasj@medi.unican.es
authors.forename: J
authors.initials: J
authors.lastname: Navas
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000472:pathogenicity; D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D006801:Humans; D016254:Mutagenesis, Insertional; D009161:Mycobacterium / Q000235:genetics; D011995:Recombination, Genetic; D013045:Species Specificity; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial