Induction of human endothelial cell growth by mildly oxidized low density lipoprotein.
The aim of the present study was to examine whether endothelial growth could be modulated by mildly oxidized low density lipoprotein. When human endothelial cells were cultured in the presence of mildly oxidized low density lipoprotein (1 microgram/ml), a significant induction of endothelial cell growth was observed, whereas native low or high density lipoprotein were ineffective. Further, treatment of endothelial cells with mildly oxidized low density lipoprotein modulated the expression of cytokines and growth factors which may be relevant in atherogenesis. Endothelial cells chronically exposed to mildly oxidized low density lipoprotein underwent a more rapid onset of cellular senescence. Since senescence is associated with endothelial dysfunction, the novel finding showing that mildly oxidized low density lipoprotein induces endothelial cell growth may be relevant in the development and evolution of the atherosclerotic lesions.
Maier
J A
JA
Dipartimento di Scienze e Technologie Biomediche, Ospedale San Raffaele, Milan, Italy. maierj@dibit.hsr.it
Barenghi
L
L
Bradamante
S
S
Pagani
F
F
D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins
D007375:Interleukin-1; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C099252:oxidized low density lipoprotein; D016173:Macrophage Colony-Stimulating Factor