A defect in beta-galactosidase of B lymphocytes in the osteopetrotic (op/op) mouse.
Macrophages were activated by administration of an inflammatory lipid metabolite, lysophosphatidylcholine (lyso-Pc), to wild type mice but not osteopetrotic op/op mice. In vitro treatment of wild type mouse peritoneal cells with lyso-Pc efficiently activated macrophages whereas lyso-Pc-treatment of op/op mouse peritoneal cells resulted in no significant activation of macrophages. Generation of macrophage activating factor requires a precursor protein, serum vitamin D3-binding protein (DBP), as well as participation of beta-galactosidase of lyso-Pc-primed B lymphocytes. The treatment of wild type mouse peritoneal cells with lyso-Pc induced beta-galactosidase of B lymphocytes leading to the conversion of DBP to macrophage activating factor and subsequent activation of macrophages. The lyso-Pc-inducible beta-galactosidase activity of B lymphocytes was found to be defective in op/op mouse.
Yamamoto
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N
Laboratory of Cancer Immunology and Molecular Biology, Albert Einstein Cancer Center, Philadglphia, PA 19141, USA.
Naraparaju
V R
VR
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology*; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D010022:Osteopetrosis / Q000201:enzymology*; D014809:Vitamin D-Binding Protein / Q000097:blood; D001616:beta-Galactosidase / Q000032:analysis*
D014809:Vitamin D-Binding Protein; D001616:beta-Galactosidase