title: The effect of infection and lag screw fixation on revascularization and new bone deposition in membranous bone grafts in a rabbit model.
abstract: We have suggested that rigid fixation of membranous bone grafts in the presence of infection may improve graft-recipient bone union by facilitating graft revascularzation. To test this hypothesis, we grafted autogenous membranous bone grafts to the mandibles of 94 New Zealand White rabbits. Lag screw fixation was applied in half the animals. The wounds were inoculated with a range of Staphylococcus aureus doses. Infected and noninfected rabbits were injected weekly over a 5-week course with fluorescein bone markers and with a marker of vascular endothelium (procion red) just prior to sacrifice. Revascularization and new bone deposition in the grafts were then quantified histologically for the 75 rabbits available for data collection. Infection decreased the amount of graft revascularized and the amount of new bone deposited for both rigidly fixated and nonfixated grafts. Grafts fixated with a lag screw showed a greater amount of revascularization and new bone deposition in the presence and absence of infection when compared with nonfixated grafts, supporting the hypothesis that rigid fixation of membranous bone grafts in the presence of infection may promote graft survival and union by improving revascularization and osteogenesis within the graft.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Toronto, Ontario, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Fialkov
authors.forename: J H
authors.initials: JH
authors.lastname: Phillips
authors.forename: S L
authors.initials: SL
authors.lastname: Walmsley
authors.forename: I
authors.initials: I
authors.lastname: Morava-Protzner
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D001863:Bone Screws; D016025:Bone Transplantation / Q000379:methods* / Q000502:physiology; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D008334:Mandible / Q000601:surgery; D011817:Rabbits; D013203:Staphylococcal Infections / Q000503:physiopathology*; D013530:Surgical Wound Infection / Q000503:physiopathology*; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology