A novel cDNA isolated from the mouse eye lens encoding a protein with zinc fingers and a KRAB domain.
A cDNA clone, pMLZ-8, was isolated from a newborn mouse eye lens cDNA library using a consensus zinc finger oligonucleotide hybridization probe. The cDNA contains an open reading frame that conceptually encodes a 606 amino acid protein. The protein possesses a "KRAB" domain at its amino-terminus and has a carboxy-terminal region with fifteen consensus C2H2 zinc fingers. PCR analysis reveals the presence of pMLZ-8 mRNA in lens, liver, kidney, spleen, and brain of newborn mice.
Brady
J P
JP
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-2730, USA.
Piatigorsky
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003459:Crystallins / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D016335:Zinc Fingers / Q000235:genetics
D003459:Crystallins; D018076:DNA, Complementary; D012333:RNA, Messenger