Detection of Neospora caninum DNA by the polymerase chain reaction.
Neospora caninum is a cyst-forming coccidian parasite which is now recognised as a major cause of abortion and neonatal mortality in cattle and other livestock. This study describes the primary DNA structure of the transcribed spacer region of the rDNA of N. caninum. Of importance is that the sequence data generated have been used to develop a species-specific PCR test for N. caninum DNA, which will prove valuable in epidemiology studies on neosporosis.
Payne
S
S
Department of Cell and Molecular Biology, University of Technology Sydney, NSW, Australia.
Ellis
J
J
D000034:Abortion, Veterinary; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases; D003048:Coccidiosis / Q000175:diagnosis / Q000401:mortality / Q000662:veterinary*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D008969:Molecular Sequence Data; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D012689:Sequence Homology, Nucleic Acid; D014122:Toxoplasma / Q000235:genetics
D017931:DNA Primers; D016054:DNA, Protozoan