title: Cross-reacting antibodies to Sarcoptes suis, Chorioptes bovis and Notoedres cati and anti-P. ovis IgE in sera from sheep infested naturally with Psoroptes ovis.
abstract: For the development of immunodiagnostic tests to detect mange mite infections in man and animals, it is necessary to know about antigen structure and cross-reactivity between different mite species and other arthropods in contact with the host. Sera from sheep infected with Psoroptes ovis (sheep anti-P. ovis sera) showed positive reactions in dot blots to P. ovis antigen and cross-reactivity to crude antigen extracts from Sarcoptes suis, Notoedres cati and Chorioptes bovis. Using sheep Specific Pathogen Free (SPF) sera in dot blots, weak reactions were seen to all but the Ch. bovis antigen. In SDS PAGE-separated P. ovis antigens at least 35 different proteins could be distinguished. In western blots, at least 24 out of these 35 were recognized as antigens by sheep anti-P. ovis immunoglobulins. At least 13 were recognized by sheep anti-P. ovis IgE. One of these, at 19 kDa, was recognized only with sheep anti-P. ovis IgE, the other 12 also with anti-P. ovis immunoglobulins. Cross-reactive antigens were recognized by sheep anti-P. ovis immunoglobulins in SDS PAGE-separated, and nitrocellulose transferred mite extracts in western blots as follows: 13 antigens in S. suis extracts, 9 in N. cati and 8 in Ch. bovis. Sheep SPF sera recognized an antigen at 67 kDa in each of these 4 mite species. Rabbit anti-sheep immunoglobulins in the P. ovis antigen control bound to a protein at 28 kDa which may be sheep IgG light chain taken up by P. ovis feeding on sheep. Despite the antigenic similarities between mange mites, sufficient differences were apparent to make immunodiagnostic tests for mange feasible.
authors.affiliation: AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: H F
authors.initials: HF
authors.lastname: Matthes
authors.forename: G B
authors.initials: GB
authors.lastname: Harrison
authors.forename: R J
authors.initials: RJ
authors.lastname: Shaw
authors.forename: A C
authors.initials: AC
authors.lastname: Heath
authors.forename: A
authors.initials: A
authors.lastname: Pfeffer
authors.forename: T H
authors.initials: TH
authors.lastname: Hiepe
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001181:Arthropods; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology* / Q000302:isolation & purification; D007147:Immunoglobulin Light Chains / Q000276:immunology; D008924:Mite Infestations / Q000276:immunology / Q000662:veterinary*; D008925:Mites / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases; D012867:Skin / Q000469:parasitology; D013045:Species Specificity
chemical_list: D000941:Antigens; D007147:Immunoglobulin Light Chains; D007073:Immunoglobulin E