Mutagenicity of cytostatic drugs in a bacterial system. II. DNA-repair test.
A liquid micromethod modification of the DNA-repair test using an automatic growth analyzer was developed. The wild strain Escherichia coli WP2 and six repair-deficient isogenic test strains were used. To compare this repair test with the conventional plate Ames test, a set of nine cytostatics were tested. Cloturin, adriamycin, mitoxantron, oracin, lomustine and tris(2-chloroethyl)amine were found to be positive, and 6-mercaptopurine, butocin and cyclophosphamide negative. The experimental micromethod appears to be useful for assessing the differential lethality in bacteria and can be combined in any short-term test system to predict genotoxicity.
Marhan
J
J
Research Institute of Pharmacy and Biochemistry Co. (VUFB), Pardubice-Rosice, Czech Republic.
D000970:Antineoplastic Agents / Q000494:pharmacology / Q000633:toxicity*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D009152:Mutagenicity Tests
D000970:Antineoplastic Agents; D004269:DNA, Bacterial