Gamma-interferon upregulates transferrin receptors and decreases membrane microviscosity in K562 cells.
We investigated the effect of gamma-interferon (gamma-IFN) on three cellular parameters: cell membrane fluidity and expression of two antigens that have been associated with cell proliferation, namely transferrin receptor (Tf-R: a cell surface protein) and Ki-67 antigen (Ki-67: a nuclear protein). We observed small, yet significant changes in the first two parameters, but not the third parameter. These were investigated in K562 cells, a human chronic myelocytic leukemia cell line. These results suggest that the microviscosity changes and the surface Tf-R density were closely associated, and that gamma-IFN was involved in increasing proliferative activity of the cells by decreasing membrane fluidity and upregulating Tf-R expression.
Iwagaki
H
H
First Department of Surgery, Okayama University Medical School, Japan.
Marutaka
M
M
Yoshino
T
T
Cao
L
L
Sakuma
I
I
Uomoto
M
M
Takeuchi
Y
Y
Tanaka
N
N
D002455:Cell Division / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D019394:Ki-67 Antigen; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D008560:Membrane Fluidity / Q000187:drug effects*; D009363:Neoplasm Proteins / Q000187:drug effects / Q000378:metabolism; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism; D011990:Receptors, Transferrin / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*; D014783:Viscosity
D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011990:Receptors, Transferrin; D007371:Interferon-gamma