Telomerase activity in plant cells.
Telomerase is a ribonucleoprotein enzyme which elongates the G-rich strand of telomeric DNA to compensate for the progressive reduction in its length due to incomplete replication of chromosome ends, which in human somatic cells leads to cell cycle arrest upon shortening of telomeres to a critical length. To examine the possible involvement of telomerase in metabolism of plant genetic material, we used cells of Nicotiana tabacum strain TBY-2, a stable long-term culture which has kept a constant pattern of restriction fragments from chromosome termini during its 6 month period of cultivation in our laboratory. In a direct assay for telomerase, a 5' end-labeled plant telomeric oligonucleotide 5' (TTTAGGG)(3')6 was elongated in a TBY-2 cell extract, showing a pausing pattern which is a characteristic feature of telomerases from other organisms. The elongation was inhibited by RNase A pretreatment of the extract. We conclude that plant cells possess telomerase which is used for maintenance of their telomeres.
Fajkus
J
J
Institute of Biophysics, Academy of Sciences of the Czech Republic, Czech Republic. fajkus@zoncna.ibp.cz
Kovarík
A
A
Královics
R
R
D001483:Base Sequence; D002460:Cell Line; D018744:DNA, Plant / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000737:chemistry; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D018744:DNA, Plant; D019098:Telomerase; D012259:Ribonuclease, Pancreatic