Dual effect of ammonium persulfate on the generation of leukotrienes from human neutrophil granulocytes.
Occupationally induced hypersensitive reactions towards chemical compounds are increasingly observed. In this regard ammonium persulfate (APS) is reported as an inducer of erythema, urticaria, eczema, rhinitis and bronchial asthma. On the basis of these reactions, persulfates obviously show some characteristics of skin-sensitizing as well as respiratory allergens. We measured the effect of APS on the release of inflammatory mediators, i.e. leukotriene B4 (LTB4), from human polymorphonuclear neutrophils (PMN). The effect of the persulfate was analyzed in comparison of the corresponding sulfate. After different cellular activation either with the Ca ionophore A23187, the tripeptide formyl-methionyl-leucyl-phenylalanine (fMLP) or sodium fluoride (NaF) coincubation of PMN with APS led to a diminished generation of LTB4. In addition it was demonstrated that APS decreased the stability of leukotrienes in cell-free systems. However, cells which were preactivated with APS and subsequently washed showed an increase in leukotriene formation after stimulation with fMLP or NaF but not with the Ca ionophore. Thus, the concentration of APS at local tissue sites as well as the occurrence and nature of a secondary cell-activating stimulus finally determine to what extent persulfates will interfere with cellular functions, e.g. mediator suppression or induction, which are then responsible for clinical disease processes.
Köller
M
M
Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr-Universität Bochum, Germany.
Hilger
R A
RA
König
W
W
D000645:Ammonium Sulfate / Q000494:pharmacology*; D002474:Cell-Free System / Q000187:drug effects; D004355:Drug Stability; D006801:Humans; D007975:Leukotriene B4 / Q000096:biosynthesis; D015289:Leukotrienes / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*
D015289:Leukotrienes; D007975:Leukotriene B4; C031276:ammonium peroxydisulfate; D000645:Ammonium Sulfate