A rapid method for detecting specific amplified PCR fragments in microtiter plates.
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.
Ortiz
A
A
CIMA-Granja Modelo, Vitoria-Gasteiz, Spain.
Ritter
E
E
D004247:DNA / Q000302:isolation & purification; D005638:Fruit / Q000235:genetics; D015698:Genomic Library; D009174:Mycoplasma / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D017800:Potyvirus / Q000235:genetics
D004247:DNA