title: Effect of X-ray irradiation on proliferation and differentiation of osteoblast.
abstract: We exposed the osteoblast-like cell line, MC3T3-E1, to 1-to 10-Gy X-ray. Irradiation at doses of 5-Gy dose or stage, confluence, and post-proliferation stages. The alkaline phosphatase activity, conversely, was increased by irradiation, and the calcium content of irradiated cells was greater than that of nonirradiated. These findings suggest that irradiation induces terminal differentiation and calcification of osteoblasts.
authors.affiliation: Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Osaka University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsumura
authors.forename: A
authors.initials: A
authors.lastname: Jikko
authors.forename: H
authors.initials: H
authors.lastname: Hiranuma
authors.forename: A
authors.initials: A
authors.lastname: Deguchi
authors.forename: H
authors.initials: H
authors.lastname: Fuchihata
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects