Gated conductances in native and reconstituted membranes from frog olfactory cilia.
Although cAMP is well established as a second messenger for olfactory transduction in vertebrates, the role of inositol 1,4,5-trisphosphate (IP3) in this process remains controversial. We addressed this issue by comparing currents evoked by cAMP and IP3 in native and reconstituted membranes from olfactory cilia. We detected only a cyclic nucleotide-gated conductance in the native membrane but both cyclic nucleotide-gated and IP3-gated conductances in the reconstituted membrane. The magnitudes of the cyclic nucleotide- and IP3-gated conductances were not correlated with each other in reconstituted membranes, suggesting that cyclic nucleotide- and IP3-gated channels originate in different cellular compartments.
Nakamura
T
T
Department of Applied Physics and Chemistry, University of Electro-Communications, Tokyo, Japan.
Lee
H H
HH
Kobayashi
H
H
Satoh
T O
TO
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002923:Cilia / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D004553:Electric Conductivity; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism; D008567:Membranes, Artificial; D009831:Olfactory Mucosa / Q000187:drug effects / Q000378:metabolism*; D011892:Rana catesbeiana; D015290:Second Messenger Systems; D015398:Signal Transduction
D007473:Ion Channels; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP