title: Titration of fresh meat color stability and malondialdehyde development with Holstein steers fed vitamin E-supplemented diets.
abstract: Pigment and lipid oxidations were investigated in longissimus lumborum (LL), semimembranosus (SM), and gluteus medius (GM) from Holstein steers fed four doses of vitamin E (64 [control], 295, 550, or 2,173 IU/d) for two durations (42 or 126d). Vitamin E dose did not affect (P = .30) carcass quality or yield characteristics. The LL was stored in vacuum packages at 4 degrees C for 14, 28, and 56 d, and GM and SM were stored for 14 d. Increments of dose and duration of vitamin E supplementation increased (P < .001) alpha-tocopherol concentration in blood plasma and in these muscles. During simulated retail display, accumulations of metmyoglobin (METMB) and thiobarbituric acid reactive substances (TBARS) were greater (P < .01) in beef from control than in beef from supplemented steers. In cubic models, muscle alpha-tocopherol accounted for 79% of the variation in TBARS and 66% of the variation in METMB. Color display life, calculated by the METMB threshold method, revealed fewer dose and duration effects of vitamin E than were evident following analysis of variance of the METMB responses. Across durations and muscles, color display-life of fresh beef calculated by the METMB threshold method was extended (P < .05) .9 to 1.8 d by vitamin E supplementation (P < .05). Storage for 28 or 56 d caused only a slight decline (P < .001) in LL alpha-tocopherol concentration but diminished (P < .05) vitamin E effects on color display-life. Although the ranking of alpha-tocopherol accumulation was GM > SM > LL, the color display-life ranking of these muscles across vitamin E treatments was LL > SM > GM.
authors.affiliation: Department of Meat and Animal Science, University of Wisconsin-Madison, 53706-1284.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: K K
authors.initials: KK
authors.lastname: Scheller
authors.forename: S C
authors.initials: SC
authors.lastname: Arp
authors.forename: D M
authors.initials: DM
authors.lastname: Schaefer
authors.forename: S N
authors.initials: SN
authors.lastname: Williams
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005524:Food Technology; D005527:Food, Fortified; D008297:Male; D008315:Malondialdehyde / Q000032:analysis* / Q000378:metabolism; D008460:Meat / Q000592:standards*; D008786:Metmyoglobin / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000737:chemistry* / Q000378:metabolism; D009211:Myoglobin / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010858:Pigmentation / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D014810:Vitamin E / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*
chemical_list: D009211:Myoglobin; D017392:Thiobarbituric Acid Reactive Substances; D008786:Metmyoglobin; D014810:Vitamin E; D008315:Malondialdehyde