Immortalization of four new Fanconi anemia fibroblast cell lines by an improved procedure.
Fanconi anemia (FA) is an autosomal recessive disease characterized by birth defects, progressive bone marrow failure and increased risk for leukemia. FA cells display chromosome breakage and increased cell killing in response to DNA crosslinking agents. At least 5 genes have been defined by cell complementation studies, but only one of these, FAC has been cloned to date. Efforts to map and isolate new FA genes by functional complementation have been hampered by the lack of immortalized FA fibroblast cell lines. Here we report the use of a novel immortalization strategy to create 4 new immortalized FA fibroblast lines, including one from the rare complementation group D.
Jakobs
P M
PM
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA.
Sahaayaruban
P
P
Saito
H
H
Reifsteck
C
C
Olson
S
S
Joenje
H
H
Moses
R E
RE
Grompe
M
M
D018929:Cell Culture Techniques; D002459:Cell Fusion; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D004852:Epoxy Compounds / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005199:Fanconi Anemia; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005816:Genetic Complementation Test; D006801:Humans; D007621:Karyotyping; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D010641:Phenotype; D012867:Skin / Q000166:cytology
D004852:Epoxy Compounds; D009153:Mutagens; D016685:Mitomycin; C007366:diepoxybutane; D005020:Ethyl Methanesulfonate