title: Plasminogen activator inhibitor type 1 in rat spermatozoa: localisation in the tail, in the acrosome and on the surface of the head.
abstract: Isolated seminiferous tubules of rat testis contain considerable urokinase-inhibiting activity. An immunohistological analysis revealed the presence of plasminogen activator inhibitor type 1 (PAI-1) in the basement membrane as well as in the interior of the tubules. Distribution and intensity of the intratubular immunoreactivity depends on the stage of the seminiferous cycle. A relatively weak signal is present around elongated nuclei of spermatids at the beginning of chromatin condensation. The signal intensity increases in the course of differentiation until a maximum is reached at stages VII-VIII. In these stages PAI-1 immunoreactivity is localised around the nuclei of the late spermatids as well as along their tails. Spermatozoa in the ductus epididymis also strongly react with the PAI-1-specific antiserum, suggesting that the inhibitor remains associated with the germ cells after spermiation and during maturation in the epididymis. In intact mature spermatozoa isolated from epididymis cauda by "swimming-up' in non-capacitation medium, PAI-1 antigen is localised on the plasma membrane surrounding the head. In addition, in fixed and permeabilised cells the immunoreactivity is detectable in the acrosome and in the tail. Possible functions of PAI-1 in spermatogenesis, sperm motility and sperm-egg interaction are discussed.
authors.affiliation: Arbeitsgruppe Zell- und Tumorbiologie, Fakultaät für Biologie, Universitaät Konstanz, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Manske
authors.forename: B
authors.initials: B
authors.lastname: Welte
authors.forename: K
authors.initials: K
authors.lastname: Hitschke
authors.forename: A
authors.initials: A
authors.lastname: Wack
authors.forename: E G
authors.initials: EG
authors.lastname: Bade
mesh_terms: D000177:Acrosome / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001485:Basement Membrane / Q000737:chemistry; D006690:Bisbenzimidazole; D002467:Cell Nucleus / Q000737:chemistry; D004822:Epididymis / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000737:chemistry; D013077:Sperm Head / Q000737:chemistry; D013082:Sperm Tail / Q000737:chemistry; D013091:Spermatogenesis; D013094:Spermatozoa / Q000737:chemistry*; D013737:Testis / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D017395:Plasminogen Activator Inhibitor 1; D006690:Bisbenzimidazole