Presence of multiple laminin- and fibronectin-binding proteins in cell wall extract of Candida albicans: influence of dialysis.
Candida albicans has been reported to express only one to three proteins that bind extracellular matrix proteins, such as laminin and fibrinogen. In those reports, cell wall extracts were subjected to various processing steps, such as dialysis and lyophilization, prior to Western blot analysis. Here, we demonstrate that dialysis for only 2 h of cell wall protein extracts results in a substantial loss (40-60%) of protein. With overnight dialysis, the loss was increased further. After 2 h of dialysis, wall extracts contained fewer laminin- and fibronectin-reactive proteins. In addition, the number of wall proteins in the extracts detected by a polyclonal anti-human fibronectin receptor antiserum decreased after dialysis. These results demonstrate that the C. albicans yeast cell wall contains multiple proteins capable of binding laminin and fibronectin and many of these proteins are not functionally detectable following dialysis.
Glee
P M
PM
Department of Pathology, University of Virginia Medical Center, Charlottesville 22908, USA.
Masuoka
J
J
Ozier
W T
WT
Hazen
K C
KC
D000818:Animals; D002176:Candida albicans / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D003956:Dialysis; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D017469:Receptors, Fibronectin / Q000302:isolation & purification*; D017474:Receptors, Laminin / Q000302:isolation & purification*; D013997:Time Factors
D007797:Laminin; D017469:Receptors, Fibronectin; D017474:Receptors, Laminin; D005340:Fibrinogen