Role of ascorbic acid in the metabolism of S-nitroso-glutathione.
Although nitric oxide (NO) has been known to generate S-nitroso-thiols (RSNOs), dynamic aspects of their metabolism remain to be elucidated. The present work reports the reactivity of S-nitroso-glutathione (GS-NO) with various compounds with reducing activity. Kinetic analysis revealed that among various reagents tested, ascorbic acid showed a potent activity to decompose GS-NO to glutathione and oxidized products of NO. During the reaction of GS-NO and ascorbic acid, monodehydroascorbate was found to appear as an intermediate. These results suggest that ascorbic acid might be an important modulator for RS-NO metabolism.
Kashiba-Iwatsuki
M
M
Department of Biochemistry, Osaka City University Medical School, Osaka, Japan.
Yamaguchi
M
M
Inoue
M
M
D001205:Ascorbic Acid / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D009603:Nitroso Compounds / Q000378:metabolism*; D026422:S-Nitrosoglutathione
D009603:Nitroso Compounds; D026422:S-Nitrosoglutathione; D005978:Glutathione; D001205:Ascorbic Acid