Spontaneous rate of sister chromatid exchanges (SCEs) and BrdU dose-response relationships in mitotic chromosomes of goat (Capra hircus L.).
The spontaneous level of sister chromatid exchange (SCEs) in the goat, estimated by exposing peripheral blood lymphocytes to 0.1 microgram/ml of 5-bromodeoxyuridine (BrdU), was 3.28 +/- 1.71 SCE/cell, 1.64 SCE/cell generation and 0.027 SCE/chromosome. The dose-response curve of SCE/cell, observed by exposing the cells to 0.1, 0.25, 0.5, 1.0, 2.5, and 5.0 micrograms/ml of BrdU, rose rapidly from 0.1 to 0.5 microgram/ml, remained fairly stable from 0.5 to 1.0 microgram/ml and rose less rapidly from 1.0 to 5.0 micrograms/ml of BrdU. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various number of exchanges followed the Poisson probability at all BrdU levels; only at 5.0 micrograms/ml of BrdU was the fit found on the border of the 5% probability level. The usefulness of determining the spontaneous level of SCE/cell in domestic animals is discussed in relation to its possible application for a more precise evaluation of the genotoxic effects of environmental pollutants.
Di Berardino
D
D
Department of Animal Science, University of Naples Federico II, Portici-Naples, Italy.
Jovino
V
V
Lioi
M B
MB
Scarfi
M R
MR
Burguete
I
I
D000818:Animals; D001973:Bromodeoxyuridine; D002875:Chromosomes; D004305:Dose-Response Relationship, Drug; D006041:Goats / Q000235:genetics*; D008938:Mitosis / Q000187:drug effects*; D012854:Sister Chromatid Exchange
D001973:Bromodeoxyuridine