Acidosis prevents growth hormone-induced growth in experimental uremia.
The effects of 2 weeks of a daily injection (2 IU/day) of recombinant human growth hormone (GH) were studied in young (60-g) growing rats in two experiments. Experiment 1 was performed in uremic animals (mean plasma creatinine 65-71 mumol/l) who were either acidotic (mean bicarbonate 11.5 mmol/l) or had acidosis corrected (mean bicarbonate 26 mmol/l) by addition of sodium bicarbonate to the diet. Experiment 2 used rats with normal renal function (plasma creatinine 25 mumol/l) who were either non-acidotic but restricted to the dietary intake of uremic rats or rendered acidotic by ammonium chloride. GH induced an increase in body weight and length in non-acidotic uremic (+33% and +41%) and in non-acidotic food-restricted (+13% and +42%) rats, associated with an increased rate of protein synthesis and little change in plasma insulin-like growth factor 1 (IGF 1). In both acidotic rat groups, GH altered none of the parameters studied. Thus: (1) the presence of severe metabolic acidosis blunts the response to GH in uremic and non-uremic rats and (2) the increment of growth rate does not depend on a rise in plasma IGF 1.
Kleinknecht
C
C
INSERM U426, Faculté Xavier Bichat, Paris, France.
Maniar
S
S
Zhou
X
X
Motel
V
V
Laouari
D
D
Yvert
J P
JP
Dechaux
M
M
D000138:Acidosis / Q000150:complications / Q000503:physiopathology*; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D004032:Diet; D006128:Growth / Q000187:drug effects / Q000502:physiology*; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D009392:Nephrectomy; D010100:Oxygen / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D017693:Sodium Bicarbonate / Q000494:pharmacology; D014511:Uremia / Q000150:complications / Q000503:physiopathology*
D000643:Ammonium Chloride; D002245:Carbon Dioxide; D007334:Insulin-Like Growth Factor I; D017693:Sodium Bicarbonate; D013006:Growth Hormone; D010100:Oxygen