Taurine- and glycine-conjugated bile acids in the preruminant goat: identification by high performance liquid chromatography.
A reversed-phase high performance liquid chromatography technique has been developed for the identification of taurine- and glycine-conjugated bile acids in the bile of preruminant goats. The mobile phase consisted of two solvents: acetonitrile (A) and 5 mM potassium phosphate buffer (pH 4.5) (B). Samples (10 microliters) were eluted with a linear gradient in which acetonitrile was increased from 25 to 35%, and from 35 to 45%, at 10 min intervals. Flow rate was 1.0 ml min-1, and bile acids were detected at 200 nm. This sample high resolution technique was highly reproducible, involved a minimum of straightforward sample treatment, and required a short chromatographic development time. The technique will be of use in the systematic identifications of bile acids in preruminants.
Fernández
J I
JI
Departamento de Fisiologia, Facultad de Farmacia, Universidad de Granada, Spain.
Mañas
M
M
Rueda
A
A
Martínez-Victoria
E
E
Naranjo
J A
JA
D000097:Acetonitriles; D000818:Animals; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000032:analysis* / Q000378:metabolism; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D005998:Glycine / Q000378:metabolism*; D006041:Goats / Q000378:metabolism*; D010710:Phosphates; D017680:Potassium Compounds; D013654:Taurine / Q000378:metabolism*
D000097:Acetonitriles; D001647:Bile Acids and Salts; D002021:Buffers; D010710:Phosphates; D017680:Potassium Compounds; D013654:Taurine; C013216:potassium phosphate; D005998:Glycine; C032159:acetonitrile