Impaired IgG-dependent anaphylaxis and Arthus reaction in Fc gamma RIII (CD16) deficient mice.
The family of receptors for IgG (Fc gamma R) plays an essential role in antibody-mediated effector functions of the immune system. However, the specific contribution of each of the Fc gamma R classes to in vivo immune reactions is still unclear. Here, we demonstrate that mice deficient for the ligand-binding alpha chain of Fc gamma RIII lack NK cell-mediated antibody-dependent cytotoxicity and phagocytosis of IgG1-coated particles by macrophages. Strikingly, these mice lack IgG-mediated mast cell degranulation, are resistant to IgG-dependent passive cutaneous anaphylaxis, and exhibit an impaired Arthus reaction. These results indicate a prominent role for Fc gamma RIII in inflammatory and anaphylactic responses, making this receptor a potential target in immunotherapy.
Hazenbos
W L
WL
Department of Immunology, University Hospital Utrecht, The Netherlands.
Gessner
J E
JE
Hofhuis
F M
FM
Kuipers
H
H
Meyer
D
D
Heijnen
I A
IA
Schmidt
R E
RE
Sandor
M
M
Capel
P J
PJ
Daëron
M
M
van de Winkel
J G
JG
Verbeek
J S
JS
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001183:Arthus Reaction / Q000276:immunology*; D015550:Cell Degranulation / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D017452:Receptors, IgG / Q000172:deficiency* / Q000235:genetics*; D012756:Sheep / Q000276:immunology
D007074:Immunoglobulin G; D017452:Receptors, IgG