title: Design of a high resolution image cytometer with open software architecture.
abstract: A relatively inexpensive, non-proprietary high resolution color pathology workstation is described. Hardware consists of a JVC frame capture camera with adjustable resolution up to 4416 x 3456, matching frame grabber and a Unix workstation. Analytic software was developed using Khoros 1.0.5, a powerful and flexible system for the development of image analysis applications that is based on a visual programming language. Applications have been developed for DNA ploidy, quantitative immunohistochemistry and texture and shape analysis. The instrument's software is uniquely extensible and transparent, and has been made publicly available over the Internet.
authors.affiliation: Department of Dermatology, Medical College of Wisconsin, Milwaukee 53226-4810, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Fleming
mesh_terms: D002468:Cell Physiological Phenomena; D048429:Cell Size; D004247:DNA / Q000032:analysis; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D008853:Microscopy / Q000295:instrumentation; D011003:Ploidies; D012984:Software
chemical_list: D004247:DNA