title: Selection of murine lymphoid and hematopoietic cells using polystyrene tissue culture devices containing covalently immobilized antibody.
abstract: We have established rapid procedures that negatively deplete and positively select for specific murine cell populations. By using polystyrene tissue culture flasks containing a covalently bound mouse anti-rat antibody and specific anti-mouse, cell-surface antigen antibodies, we easily and efficiently depleted greater than 90% of the mature lineage cells from murine bone marrow. This selection procedure resulted in an enrichment of progenitor colonies (CFU-Cs) in murine bone marrow. Using the same polystyrene tissue culture devices, we can directly isolate CD117+ (c-kit+) murine hematopoietic cells. As few as 2000 of these CD117+ cells rescued and reconstituted lethally irradiated recipients in a murine bone marrow transplant model.
authors.affiliation: Applied Immune Sciences, Inc., Santa Clara, CA 95054-1114 USA. lebkowsk@aisx.com
authors.forename: A E
authors.initials: AE
authors.lastname: Berson
authors.forename: K M
authors.initials: KM
authors.lastname: Knobel
authors.forename: D
authors.initials: D
authors.lastname: Rood
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Lamons
authors.forename: M A
authors.initials: MA
authors.lastname: McNally
authors.forename: T B
authors.initials: TB
authors.lastname: Okarma
authors.forename: J S
authors.initials: JS
authors.lastname: Lebkowski
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology
chemical_list: D019009:Proto-Oncogene Proteins c-kit