Specific interaction with guanine residues of Z-form DNA by bleomycin-nickel(III) complex.
The Ni(III) complex of bleomycin (BLM) reacts with guanine residues of the oligomer duplex only under high salt condition (4.5 M NaCl) where the (dC-dG)6 sequence exists as the isolated Z-form segment. In addition, the (dC-dG)n inserts were subcloned into a plasmid so that reactivity of the BLM-Ni(III) complex with restriction fragments could be examined. Results are reported for two fragments: an EcoRI-DdeI fragment in which the (dC-dG)n insert is flanked at the 5' end by a segment of B-DNA and a HindIII-DdeI fragment in which the (dC-dG)n fragment is embedded at both ends by stable B-DNA forming segments. The BLM-Ni(III) complex does not bind to (dC-dG)5 sequence of pBRZ10 DNA fragment under any conditions, but to several guanine residues of (dC-dG)8 and (dC-dG)12 sequences of pBRZ16 and pBRZ24 DNA fragments in 4.5 M NaCl solution. In the fragments of pBRZ16 and pBRZ24 DNAs, the BLM-Ni(III) complex reacts with most of guanine bases of (dC-dG)8 and (dC-dG)12.
Guan
L L
LL
Institute for Chemical Research, Kyoto University, Japan.
Morii
T
T
Otsuka
M
M
Sugiura
Y
Y
D001483:Base Sequence; D001665:Binding Sites; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; C048319:nickel bleomycin; D001761:Bleomycin; D006147:Guanine; D004247:DNA