Simple method for analysis of diquat in biological fluids and tissues by high-performance liquid chromatography.
A simple HPLC method has been described to quantify diquat in biological fluids and tissues. This method permits separation and quantification of diquat from blood, bile, urine, liver and kidney. It does not require special pretreatment of the samples prior to analysis, nor a specially prepared analytical column. Various concentrations of diquat were added (10-300 nmol/ml or g) to fluids or tissues. Analysis of blank samples revealed no substances that interfere with diquat elution. Excellent recovery (95-105%) was obtained. Diquat (120 mumol/kg, i.v.) was injected to rats and quantified in bile, blood and liver. Concentration of diquat was higher in blood and bile than liver. Therefore, this method is applicable for quantification of diquat in toxicological samples, and may be used to determine structurally similar compounds such as paraquat.
Madhu
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Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160-7417, USA.
Gregus
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Klaassen
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D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004178:Diquat / Q000032:analysis* / Q000097:blood / Q000652:urine; D006540:Herbicides / Q000032:analysis* / Q000097:blood / Q000652:urine; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D006540:Herbicides; D004178:Diquat