The influence of essential phospholipids (ESSENTIALE) on liver regeneration in gamma irradiated rats.
Gamma irradiation with a dose of 5.7 Gy within 30 min before partial hepatectomy (PHE) caused latent damage in the intact rat liver. This was expressed in the course of proliferation induced in the liver remnant by inhibition of the regenerative process, which was indicated by a decreased mitotic index and cellularity, an increased ratio of metaphases/prophases and a high chromosomal aberration frequency. The preparation of essential phospholipids (ESSENTIALE) that was injected in a dose of 360 mg/kg (i.p.) either 24 h before irradiation or 30 min after irradiation or repeatedly before and after irradiation, markedly stimulated the process of liver regeneration after PHE in both nonirradiated and irradiated rats. It moderated all the alterations induced by irradiation, especially changes in cellularity. The most effective was the repeated administration of ESSENTIALE whereas its single administration before irradiation was more effective than that after irradiation. Our results suggest that ESSENTIALE has not only a stabilizing effect on cell membranes, but also mitigates damage of genetic material induced by irradiation.
Kropácová
K
K
Department of Cellular and Molecular Biology, Safárik University, Kosice, Slovak Republic.
Misúrová
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002869:Chromosome Aberrations; D005720:Gamma Rays; D006498:Hepatectomy; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D008115:Liver Regeneration / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008677:Metaphase / Q000187:drug effects / Q000528:radiation effects; D009929:Organ Size / Q000187:drug effects; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology*; D011418:Prophase / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology
D010713:Phosphatidylcholines; C011246:essential 303 forte; D014810:Vitamin E