title: Assignment of the hypoxia-inducible factor 1alpha gene to a region of conserved synteny on mouse chromosome 12 and human chromosome 14q.
abstract: Hypoxia-inducible factor 1 (HIF-1) is a basic helix-loop-helix transcription factor that mediates homeostatic responses to hypoxia. HIF-1 is a heterodimer consisting of HIF-1alpha, which is encoded by the HIF1A gene, complexed with HIF-1beta, which is encoded by the ARNT gene. In this paper we report the assignment of Hif1a and HIF1A to mouse chromosome 12 and human chromosome 14, respectively. HIF1A was assigned to human chromosome 14q21-q24 by analysis of somatic cell hybrids and by fluorescence in situ hybridization. Hif1a was localized by interspecific backcross analysis within a region of mouse chromosome 12 encompassing >30 cM that demonstrates conservation of synteny with a region of human chromosome 14 extending from PAX9 at 14q12-q13 to IGHC at 14q32.33.
authors.affiliation: Center for Medical Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21287, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Semenza
authors.forename: E A
authors.initials: EA
authors.lastname: Rue
authors.forename: N V
authors.initials: NV
authors.lastname: Iyer
authors.forename: M G
authors.initials: MG
authors.lastname: Pang
authors.forename: W G
authors.initials: WG
authors.lastname: Kearns
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D016001:Confidence Intervals; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D005819:Genetic Markers; D018257:Helix-Loop-Helix Motifs; D006706:Homeostasis; D006801:Humans; D006822:Hybrid Cells; D000860:Hypoxia; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012377:Rodentia; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers; C497442:HIF1A protein, human; C497443:Hif1a protein, mouse; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors