title: Biosynthesis of 17,20 alpha-dihydroxy-4-pregnen-3-one, 17,20 beta-dihydroxy-4-pregnen-3-one, and 11-ketotestosterone by testicular fragments and sperm of the roach, Rutilus rutilus.
abstract: The effect of incubation time and substrate concentration on the in vitro metabolism of 17-hydroxyprogesterone by testes of the roach has been examined. There was a shift from synthesis of the 11-oxygenated androgens, 11-ketotestosterone, and androstenetrione at low substrate concentration to 17,20 alpha-dihydroxy-4-pregnen-3-one (17,20 alpha P) at high substrate. Glucuronides and sulfates were of significant importance only at low substrate and long incubation times. There was a shift from 17,20 beta-dihydroxy-4-pregnen-3-one to 17,20 alpha P with increased substrate. The results confirm that substrate affects the steroidogenic profile and that 3 hr is optimal time for such studies. Incubations of sperm with 17-hydroxyprogesterone gave predominantly 11-ketotestosterone at low substrate concentrations and 17,20 alpha P at high substrate. The synthesis of 11-ketotestosterone is demonstrated for the first time in teleost sperm.
authors.affiliation: Department of Animal and Plant Sciences, University of Sheffield, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Ebrahimi
authors.forename: P B
authors.initials: PB
authors.lastname: Singh
authors.forename: D E
authors.initials: DE
authors.lastname: Kime
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D006908:Hydroxyprogesterones / Q000378:metabolism*; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D006908:Hydroxyprogesterones; C007433:17,20-dihydroxy-4-pregnen-3-one; D013739:Testosterone; C003600:11-ketotestosterone