Evaluation of new approaches to poliovirus vaccine neurovirulence tests.
Transgenic mice that express the human receptor for poliovirus and mutant analysis by PCR followed by restriction enzyme cleavage (MAPREC) are two new approaches to poliovirus vaccine neurovirulence testing. This review assesses the validity and current status of these tests. Both approaches require further rigorous validation and development. They are most likely to be used as corollary rather than replacement tests for the current neurovirulence tests.
Wood
D J
DJ
National Institute for Biological Standards and Control, Potters Bar Herts, UK.
D000826:Animal Testing Alternatives; D000818:Animals; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D011054:Poliovirus Vaccine, Inactivated / Q000235:genetics / Q000592:standards / Q000633:toxicity*; D011055:Poliovirus Vaccine, Oral / Q000235:genetics / Q000592:standards / Q000633:toxicity; D016133:Polymerase Chain Reaction / Q000379:methods; D011323:Primates; D011786:Quality Control; D011991:Receptors, Virus / Q000235:genetics; D014774:Virulence / Q000235:genetics
D011054:Poliovirus Vaccine, Inactivated; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus