Inhibition of L-threonine intestinal absorption in rabbits by cadmium.
Cadmium compounds are widely spread in the environment. Animal exposure to cadmium compounds occurs mainly through foods or drinks contaminated by this metal. Cadmium has been shown to produce several negative effects on the gastrointestinal tract such as inhibition on sugars and amino acids absorption. The aim of the present work was to study the inhibitory characteristics of cadmium on L-threonine intestinal absorption in rabbits in order to understand about this malabsorption of nutrients. Our results show that L-threonine tissue accumulation as well as mucosal to serosal transepithelial fluxes are decreased in a dose-dependent manner in rabbit jejunum. Amino acid diffusion across the intestinal epithelium was not affected by cadmium. A noncompetitive mechanism and a partial reversion by dithioerythritol (thiol groups protector) is described for this inhibition.
Mesonero
J E
JE
Departamento de Farmacología y Fisiología, Facultad de Veterinaria, Zaragoza, Spain.
Rodriguez Yoldi
M C
MC
Rodriguez Yoldi
M J
MJ
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011817:Rabbits; D012704:Serous Membrane / Q000378:metabolism; D013912:Threonine / Q000493:pharmacokinetics*
D002104:Cadmium; D013912:Threonine