Isolation and characterization of a cDNA encoding a sulfate transporter from Arabidopsis thaliana.
A cDNA encoding a sulfate transporter was isolated from Arabidopsis thaliana. The isolated clone contained an open reading frame encoding a polypeptide of 658 amino acids, exhibiting the highest similarity (62%) with the sequence of the low-affinity sulfate transporter of a tropical legume Stylosanthes hamata. Northern blot analysis indicated the constitutive accumulation of a 2.6 kb length transcript in leaves and roots of seedlings. We also propose that A. thaliana contains three sulfate transporter genes which are expressed as 3.0, 2.7 and 2.6 kb length transcripts, respectively, in an organ-specific manner.
Takahashi
H
H
Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology in Research Center of Medicinal Resources, Chiba University, Japan.
Sasakura
N
N
Noji
M
M
Saito
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D006801:Humans; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D000076988:Sulfate Transporters; D013455:Sulfur / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D026901:Membrane Transport Proteins; C085609:SLC26A4 protein, human; D000076988:Sulfate Transporters; D013455:Sulfur