title: New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins.
abstract: A new gene encoding a 35.8-kDa mosquitocidal toxin (Mtx3; 326 amino acids) was isolated from Bacillus sphaericus SSII-1 DNA. Mtx3 is a new type of mosquitocidal toxin with homology to the Mtx2 mosquitocidal toxin of B. sphaericus SSII-1, the epsilon-toxin of Clostridium perfringens, and the cytotoxin of Pseudomonas aeruginosa. The mtx3 gene is highly conserved and widely distributed in both high- and low-toxicity mosquito larvicidal strains of B. sphaericus.
authors.affiliation: Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.
authors.forename: J W
authors.initials: JW
authors.lastname: Liu
authors.forename: A G
authors.initials: AG
authors.lastname: Porter
authors.forename: B Y
authors.initials: BY
authors.lastname: Wee
authors.forename: T
authors.initials: T
authors.lastname: Thanabalu
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003016:Clostridium perfringens / Q000235:genetics; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005982:Glutathione Transferase / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011993:Recombinant Fusion Proteins; C068603:mosquitocidal toxin SSII-1; D005982:Glutathione Transferase