title: Comparative genome mapping of the ataxia-telangiectasia region in mouse, rat, and Syrian hamster.
abstract: Chromosomal locations of the Atm (ataxia-telangiectasia (AT)-mutated) and Acat1 (mitochondrial acetoacetyl-CoA thiolase) genes in mouse, rat, and Syrian hamster were determined by direct R-banding FISH. Both genes were colocalized to the C-D band of mouse chromosome 9, the proximal end of q24.1 of rat chromosome 8, and qa4-qa5 of Syrian hamster chromosome 12. The regions in the mouse and rat were homologous to human chromosome 11q. Fine genetic linkage mapping of the mouse AT region was performed using the interspecific backcross mice. Atm, Acat1, and Npat, which is a new gene isolated from the AT region, and 12 flanking microsatellite DNA markers were examined. No recombinations were found among the Atm, Npat, Acat1, and D9Mit6 loci, and these loci were mapped 2.0 cM distal to D9Mit99 and 1.3 cM proximal to D9Mit102. Comparison of the linkage map of mouse chromosome 9 (MMU9) and that of human chromosome 11 (HSA11) indicates that there is a chromosomal rearrangement due to an inversion between Ets1 and Atm-Npat-Acat1 and that the inversion of MMU9 originated from the chromosomal breakage at the boundary between Gria4 and Atm-Npat-Acat1 on HSA11. This type of inversion appeared to be conserved in the three rodent species, mouse, rat, and Syrian hamster, using additional comparative mapping data with the Rck gene.
authors.affiliation: Genome Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba, 263, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Shiomi
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Yamauchi
authors.forename: T
authors.initials: T
authors.lastname: Fukao
authors.forename: Y
authors.initials: Y
authors.lastname: Akao
authors.forename: N
authors.initials: N
authors.lastname: Seki
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: T A
authors.initials: TA
authors.lastname: Hori
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics*; D000818:Animals; D001260:Ataxia Telangiectasia / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000201:enzymology; D009115:Muridae; D016133:Polymerase Chain Reaction; D051381:Rats; D013045:Species Specificity
chemical_list: D005819:Genetic Markers; D000101:Acetyl-CoA C-Acetyltransferase