Identification of the nuclear transcription factor NFkappaB in rat after in vivo ethanol administration.
NFkappaB, a nuclear transcription factor, was induced in the brain nuclear fraction of naive rats after an acute injection of ethanol, 2 g/kg. In contrast, rats which had been chronically alcoholised showed the constitutively active NFkappaB-like complex only after a further acute dose of ethanol. Hepatic nuclear fractions did not exhibit the specific NFkappaB-like complex during the first 45 min after acute ethanol injection, beyond that which was normally constitutively present. Such activation of NFkappaB-like complex in the brains of the naive rats may play an important role in the cellular protective response.
Ward
R J
RJ
Laboratoire de Psychobiologie, Catholique Université de Louvain, Louvain-la-Neuve, Belgium.
Zhang
Y
Y
Crichton
R R
RR
Piret
B
B
Piette
J
J
de Witte
P
P
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000187:drug effects*; D002467:Cell Nucleus / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D016328:NF-kappa B; D000431:Ethanol