Relationships between the morphology and function of gastric and intestinal distention-sensitive neurons in the dorsal motor nucleus of the vagus.
The activity of vagal motor neurons is influenced by sensory information transmitted to the brainstem. In particular, there is evidence that distention of the stomach increases activity of motor neurons in the dorsal vagal motor nucleus, whereas distention of the duodenum, small intestine, and colon reduces neuron firing. In this study, we determined 1) the response of vagal motor neurons to distention of the stomach and duodenum and 2) whether the response properties were associated with specific morphological features. Using the single-cell recording and iontophoretic injection technique, we identified four groups of vagal motor neurons affected by gastric and/or duodenal distention. Group 1 neurons responded to either gastric or duodenal stimulation. Neurons in groups 2, 3, and 4 were affected by both gastric and duodenal distention. Group 2 neurons were excited by duodenal distention and were inhibited by gastric distention. Group 3 neurons were inhibited by duodenal distention and were excited by gastric distention. Most neurons belonged to group 4. Neurons in this group were inhibited by both gastric and duodenal distention. Our analyses revealed that the neurons affected by both stimuli had distinctive structural features. Neurons in group 2 had the largest somata, the most dendritic branches, and the greatest cell surface area. Neurons in group 3 were the smallest and had the shortest dendritic length. In addition, we were able to demonstrate that the neurons in group 4 had a smaller total dendritic length and a smaller cell volume than neurons in group 2 and had more dendritic branch segments than neurons in group 3. These results suggest that morphological features are associated with specific response properties of vagal motor neurons.
Fogel
R
R
Division of Gastroenterology, Henry Ford Health Sciences Center, Detroit, Michigan 48202, USA.
Zhang
X
X
Renehan
W E
WE
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D001933:Brain Stem / Q000166:cytology*; D048429:Cell Size / Q000502:physiology; D003712:Dendrites / Q000502:physiology; D004386:Duodenum / Q000294:innervation / Q000502:physiology; D004594:Electrophysiology; D007422:Intestines / Q000166:cytology / Q000294:innervation*; D009434:Neural Pathways; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D011312:Pressure; D051381:Rats; D013270:Stomach / Q000166:cytology / Q000294:innervation*; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
C070614:neurobiotin; D001710:Biotin