Genes expressed in Brugia malayi infective third stage larvae.
We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos, and converted to cDNA by reverse transcriptase. Double-stranded cDNA was produced by either conventional methods (non-SL cDNA library) or by PCR using the nematode spliced leader (SLI) and oligo(dT) primers (SL cDNA library). Two clone libraries (one from SL and one from non-SL cDNAs) were constructed in lambda ZapII. A set of these full-length clones was selected and 596 inserts were sequenced from the 5' end. We have identified 364 B. malayi genes (the majority of which are new) that encode housekeeping proteins, structural proteins, proteins of immediate immunological or drug-discovery interest as well as a large class of novel sequences which may prove to have significant involvement in host invasion. Extensive, genome-wide approaches to the analysis of larval gene expression are now possible for B. malayi. We present several examples of this approach.
Blaxter
M L
ML
Institute of Cell, Animal and Population Biology, University of Edinburgh, UK. mark.blaxter@ed.ac.uk
Raghavan
N
N
Ghosh
I
I
Guiliano
D
D
Lu
W
W
Williams
S A
SA
Slatko
B
B
Scott
A L
AL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017178:Brugia malayi / Q000235:genetics / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary; D004605:Elephantiasis, Filarial; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017238:Genes, Helminth; D006801:Humans; D007438:Introns; D007814:Larva; D008251:Macaca; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D021382:Protein Sorting Signals