Recovery of glycolysis and oxidative metabolism during postischemic reperfusion of hypertrophied rat hearts.
We investigated the source and extent of recovery of ATP production during postischemic reperfusion of isolated working hearts from abdominal aortic-banded rats. Rates of glycolysis, glucose oxidation, lactate oxidation, and palmitate oxidation were measured in hypertrophied and control hearts [perfused with (in mM) 11 glucose, 0.5 lactate, and 1.2 palmitate] during and after 30 min of no-flow ischemia. In the initial aerobic period glycolytic rates were 1.87-fold higher in hypertrophied hearts compared with control hearts (P < 0.05), with rates of carbohydrate and palmitate oxidation being similar. During reperfusion, hypertrophied hearts recovered 40% of preischemic function compared with 71% in control hearts. Rates of glycolysis during reperfusion of hypertrophied hearts remained accelerated compared with control hearts (2.01-fold higher, P < 0.05), whereas oxidative metabolism returned to preischemic values in both groups. The efficiency of converting ATP production into mechanical work decreased to 29% of preischemic values in hypertrophied hearts during the postischemic reperfusion compared with a decrease to only 59% of preischemic values in control hearts. This suggests that the recovery of glycolysis and oxidative metabolism in the hypertrophied heart during postischemic reperfusion is not impaired, but rather the efficiency of converting ATP produced into mechanical function decreases.
Schönekess
B O
BO
Department of Pharmacology, University of Alberta, Edmonton, Canada.
Allard
M F
MF
Lopaschuk
G D
GD
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001011:Aorta; D006332:Cardiomegaly / Q000378:metabolism* / Q000503:physiopathology; D003250:Constriction; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006321:Heart / Q000503:physiopathology; D006706:Homeostasis; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY
D010168:Palmitates; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose