Structural and serological characterisation of two O-specific polysaccharides of Acinetobacter.
Extraction of dry bacteria of Acinetobacter strain 34 (DNA group 2) or Acinetobacter strain 108 (DNA group 13) by phenol/water yielded a polymer that was identified by means of serological studies and fatty acid analysis as S-form lipopolysaccharide. Degradation of the lipopolysaccharides of strains 34 and 108 in 1% acetic acid and 5% acetic acid, respectively, and gel-permeation chromatography gave the respective O-antigenic polysaccharides, the structures of which were determined, by compositional analysis and NMR spectroscopy of the polysaccharide, as [Sequence: see text] for strain 108, where D-Fucp3NBuOH represents 3-[(R)-3-hydroxybutyramido] -3,6-dideoxy-D-galactose and D-GalpANAc represents 2-acetamido-2-deoxy-D-galacturonic acid. Both structures were specifically recognised in Western blots by polyclonal rabbit antisera and there was no cross-reaction between these two structures.
Vinogradov
E V
EV
Division of Biochemical Microbiology, Research Center Borstel, Germany.
Pantophlet
R
R
Dijkshoorn
L
L
Brade
L
L
Holst
O
O
Brade
H
H
D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D000907:Antibodies, Bacterial / Q000276:immunology; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D017421:Sequence Analysis; D012699:Serology; D013045:Species Specificity
D000907:Antibodies, Bacterial; D019081:O Antigens