Ataxia telangiectasia heterozygotes and patients display increased fluidity and decrease in contents of sulfhydryl groups in red blood cell membranes.
In red blood cell membranes of ataxia telangiectasia mutated (ATM) homozygotes and heterozygotes, decreased values of the corrected fluorescence anisotropy and the anisotropy parameter were found, indicating increased fluidity and decreased microviscosity, respectively. These changes in membranes were accompanied by a decrease in SH-groups and an increase in malondialdehyde (MDA) contents. The content of MDA both in homozygotes and in heterozygotes exceeded roughly threefold the respective control values. Decreased content of GSH in red blood cells was found only in ATM homozygotes. The change most specific for the ATM gene appears to be the increase in fluidity, since only this parameter displays the proportionally greater changes in ATM homozygotes compared to ATM heterozygotes. The observations presented here may indicate that the ATM gene is expressed in precursors of red blood cells and deficiency of normal AT gene function may produce the changes which persist in circulating cells.
Rybczyńska
M
M
Department of Clinical Chemistry, K. Marcinkowski University of Medical Sciences, Poznań, Poland.
Pawlak
A L
AL
Sikorska
E
E
Ignatowicz
R
R
D001260:Ataxia Telangiectasia / Q000097:blood* / Q000235:genetics; D002648:Child; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000502:physiology*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005978:Glutathione / Q000097:blood; D006579:Heterozygote; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D008560:Membrane Fluidity; D013438:Sulfhydryl Compounds / Q000097:blood*; D014783:Viscosity
D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D004161:Diphenylhexatriene; D008315:Malondialdehyde; D005978:Glutathione