title: Molecular cloning of, and phylogenetic analysis of, an actin in Naegleria fowleri.
abstract: We cloned and sequenced an intronless actin gene from the amoebo-flagellate Naegleria fowleri, LEE strain, an opportunistic pathogen of man. Codon usage and third-position-codon nucleotide frequency were significantly different from Acanthamoeba, another amoeba genus which also includes opportunistic pathogens of man. Between the two amoebae, actin peptide sequences were 92.8% similar, while nucleotide sequences were only 70% similar. A phylogenetic reconstruction of actin amino acid sequences, using a distance method, placed Naegleria in a cluster with Plasmodium and Entamoeba.
authors.affiliation: Department of Zoology, Michigan State University, East Lansing 48824-1115, USA.
authors.forename: S
authors.initials: S
authors.lastname: Gorospe
authors.forename: R N
authors.initials: RN
authors.lastname: Band
authors.forename: W J
authors.initials: WJ
authors.lastname: Kopachik
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016848:Naegleria fowleri / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny
chemical_list: D000199:Actins