The usefulness of a rapid PCR methodology to detect rearranged Ig heavy chain genes in lymphoproliferative disease in a diagnostic setting.
Polymerase chain reaction (PCR) was used to amplify the DNA fragments of the framework 3 region (FR3) of the immunoglobulin heavy (IgH) chain genes, from the tissue of 66 patients with B-lymphoproliferative diseases and 74 patients with other malignant diseases, reactive or normal tissue. The assay performed with 77% sensitivity, 100% specificity and 89% efficacy. In addition, the PCR assay cost less than 25% of the cost performing Southern blot analysis of tumor DNA, which has been the test performed to date, and had a turn around time of 24 hrs rather than the 7-14 days required to obtain a result from Southern blot analysis. These results suggest that PCR analysis of B-cell lymphoproliferative disease is superior to Southern blot analysis, in the setting of a diagnostic laboratory.
Angulo
R I
RI
Department of Immunopathology, ICPMR, Westmead, NSW.
Bennetts
B H
BH
Benson
E M
EM
Tschuchnigg
M S
MS
Kamath
S
S
D001483:Base Sequence; D015139:Blotting, Southern / Q000191:economics; D003363:Cost Control; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004273:DNA, Neoplasm