Capillary electrophoresis-laser-induced fluorescence detection of amphetamine in the brain.
Prefrontal cortex microdialysis was done in rats that had received intraperitoneal amphetamine (AMPH). Samples were derivatized with 10(-4) M fluorescein isothiocyanate and incubated for 18 h. AMPH was separated by capillary electrophoresis (CE) and detected by laser-induced fluorescence detection (LIFD) from 30 to 150 min after injection. The limit of mass detection was 3 amol, which is three orders of magnitude lower than that in gas chromatography-mass spectrometry, and the limit of concentration detection was 3 x 10(-9) M. The results showed that CE-LIFD is a good method for detecting AMPH in brain dialysates of rats.
Páez
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X
Department of Physiology, School of Medicine, Universidad de los Andes, Mérida, Venezuela.
Rada
P
P
Tucci
S
S
Rodríguez
N
N
Hernández
L
L
D000661:Amphetamine / Q000008:administration & dosage / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008297:Male; D017397:Prefrontal Cortex / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D005456:Fluorescent Dyes; D000661:Amphetamine; D016650:Fluorescein-5-isothiocyanate