Molecular cloning of the lectin and a lectin-related protein from common Solomon's seal (Polygonatum multiflorum).
The most prominent protein of Polygonatum multiflorum (common Solomon's seal) rhizomes has been identified as a mannose-binding lectin. Analysis of the purified lectin demonstrated that it is a tetramer of four identical subunits of 14 kDa. Molecular cloning further revealed that the lectin from this typical Liliaceae species belongs to the superfamily of monocot mannose-binding proteins. Screening of cDNA libraries constructed with RNA isolated from buds, leaves and flowers of P. multiflorum also yielded cDNA clones encoding a protein, which contains two tandemly arranged domains with an obvious sequence homology to the mannose-binding lectins. Molecular modelling of the Polygonatum lectin and lectin-related protein indicated that the three-dimensional structure of both proteins strongly resembles that of the snowdrop lectin. In addition, this approach suggested that the presumed carbohydrate-binding sites of the lectin can accommodate a mannose residue whereas most of the carbohydrate-binding sites of the lectin-related protein cannot.
Van Damme
E J
EJ
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium.
Barre
A
A
Rougé
P
P
Van Leuven
F
F
Balzarini
J
J
Peumans
W J
WJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D037102:Lectins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D037241:Mannose-Binding Lectins; D008961:Models, Structural; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D010802:Phylogeny; D037121:Plant Lectins; D018517:Plant Roots; D010944:Plants / Q000378:metabolism*; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D037102:Lectins; D037241:Mannose-Binding Lectins; D015345:Oligonucleotide Probes; D037121:Plant Lectins; D012333:RNA, Messenger; C101306:lectin-related protein, Polygonatum