title: Screening of selected basidiomycetes for inhibitory activity on neutral endopeptidase (NEP) and angiotensin-converting enzyme (ACE).
abstract: Aqueous extracts of 27 basidiomycetes were investigated for their ability to inhibit the activity of angiotensin-converting enzyme (ACE) and neutral endopeptidase (NEP). The extracts of 5 fungi inhibited both, ACE and NEP activity, another 18 extracts showed inhibition of the NEP activity whereas only 1 basidiomycete inhibited the ACE activity exclusively. The IC50 values for the ACE inhibition are rather high (between 200 and 1500 micrograms/ml) in comparison to the IC50 of the NEP inhibition (between 40 and 2000 micrograms/ml). These results indicate that the basidiomycetes investigated seem to have a higher potential for the inhibition of the activity of NEP than of ACE. In general, basidiomycetes are a new source for inhibitors of metalloendopeptidases. Resulting from the isolation and characterization of these compounds new leading structures are expectable.
authors.affiliation: Institute for Molecular Pharmacology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: M F
authors.initials: MF
authors.lastname: Melzig
authors.forename: S
authors.initials: S
authors.lastname: Pieper
authors.forename: W E
authors.initials: WE
authors.lastname: Siems
authors.forename: G
authors.initials: G
authors.lastname: Heder
authors.forename: A
authors.initials: A
authors.lastname: Bóttger
authors.forename: K
authors.initials: K
authors.lastname: Liberra
authors.forename: U
authors.initials: U
authors.lastname: Lindequist
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D015260:Neprilysin / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004791:Enzyme Inhibitors; D011480:Protease Inhibitors; D015260:Neprilysin