Determination of IAA in brassinolide treated coleoptiles of wheat by a modified indirect ELISA with polyclonal antibodies.
An indirect enzyme immunoassay for detection of as little as 10-50 pmole IAA is described for the first time. The assay is based on the development of highly specific polyclonal antibodies against the carboxyl site of IAA. The binding specificity is nearly as high as the radioimmunoassay and the titre of the specific antibody was also remarkably high (1:40,000 of the primary antibody). Such an easy, rapid, specific and highly sensitive assay would be extremely useful in gaining more information on the mode of action of phytohormones, and their effects on physiological processes.
Naren
A
A
Department of Crop Physiology, University of Agricultural Sciences, Bangalore, India.
Prasad
T G
TG
Kumar
M U
MU
Sashidhar
V R
VR
D000818:Animals; D000906:Antibodies; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D018548:Cotyledon / Q000187:drug effects* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007210:Indoleacetic Acids / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D011817:Rabbits; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism
D000906:Antibodies; D060406:Brassinosteroids; D002777:Cholestanols; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; C030737:indoleacetic acid; C023623:brassinolide