title: Basic fibroblast growth factor (bFGF) in the synovial-like membrane around loose total hip prostheses.
abstract: The aim of this investigation was to examine the eventual presence of bFGF in the synovial-like membrane of the interface and pseudocapsular tissue of loose total hip replacement (THR) and compare it to control knee synovial membrane. bFGF was demonstrated using specific antibodies in avidin-biotin-peroxidase complex (ABC) staining and quantitated using a semiautomatic VIDAS image analysis system. bFGF was found in fibroblasts, in vascular endothelial cells and in particular in macrophages in a characteristic pattern. The number of bFGF positive cells per one mm2 was increased in interface (1693 +/- 291; n = 10; p < 0.01) and pseudocapsular tissue (1954 +/- 256; n = 10; p < 0.01), compared to the knee joint synovial membrane (1009 +/- 133; n = 10). These findings suggest that bFGF is involved in the enhanced tissue remodelling of the synovial-like membrane around loose total hip prostheses. Codistribution of metallosis and bFGF positive macrophages suggests a chronic foreign body type reaction as the driving stimulus.
authors.affiliation: Molecular Biology Laboratory, University of Helsinki, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Waris
authors.forename: J W
authors.initials: JW
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Nordsletten
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: S
authors.initials: S
authors.lastname: Santavirta
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
mesh_terms: D000328:Adult; D000368:Aged; D001862:Bone Resorption / Q000276:immunology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D006622:Hip Prosthesis / Q000009:adverse effects; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013583:Synovial Membrane / Q000737:chemistry* / Q000276:immunology* / Q000473:pathology
chemical_list: D016222:Fibroblast Growth Factor 2