The stimulation and inhibition of T cell proliferation by Helicobacter pylori components.
The purified T cells from peripheral blood of healthy human volunteers, seronegative for anti-Helicobacter pylori antibody were stimulated in cultures with live or heat-killed H. pylori rods or with bacterial sialic acid-specific surface haemagglutinin (sHA), a crude surface (SF) or cytoplasmic (CF) fractions. It is demonstrated that H. pylori bacteria contain both stimulatory and inhibitory components for T cells of healthy individuals. The sHA as well as SF (5-20 micrograms) induced the proliferative response of T lymphocytes. By contrast, CF inhibited in dose dependent manner, the proliferation of T cells in the cultures stimulated with H. pylori bacteria or PHA. The result suggest that in vivo, a dominance of activation or immunosuppression could depend on the concentration of the bacteria and their products in infective foci.
Chmiela
M
M
Department of Infectious Biology, University of Lodz, Poland.
Lelwala-Guruge
J A
JA
Wadström
T
T
Rudnicka
W
W
D000328:Adult; D002455:Cell Division / Q000276:immunology; D002458:Cell Fractionation; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D016480:Helicobacter pylori / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D010835:Phytohemagglutinins / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D010835:Phytohemagglutinins