[Determination of 2,4-dichlorophenoxyacetic acid using monoclonal antibodies and a biotin-streptavidin detection system].
Simple methods to determine 2,4-dichlorophenoxyacetic acid (2,4-D) using biotinylated monoclonal antibodies in combination with a highly active streptavidin-peroxidase conjugate were developed on the basis of the immunofiltration dot assay and competitive enzyme immunoassay (EIA). The detection limit of 2,4-D acid for immunofiltration and EIA was 2-3 ng/ml, the total assay duration being 5 min and 1.5 h, respectively.
Pavlova
I S
IS
Liubavina
I A
IA
Lukin
Iu V
IuV
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D019809:Streptavidin; D013997:Time Factors
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D015084:2,4-Dichlorophenoxyacetic Acid; D001710:Biotin; D019809:Streptavidin
Opredelenie 2,4-dikhlorfenoksiukususno i kisloty s ispol'zovaniem monoklonal'nykh antitel i biotin-streptavidinovo i sistemy detektsii.