title: Frequency-dependent interference by magnetic fields of nerve growth factor-induced neurite outgrowth in PC-12 cells.
abstract: We have shown that 50 Hz sinusoidal magnetic fields within the 5-10 microTesla (microT) rms range cause an intensity-dependent reduction in nerve growth factor (NGF) stimulation of neurite outgrowth (NO) in PC-12 cells. Here we report on the frequency dependence of this response over the 15-70 Hz range at 5 Hz intervals. Primed PC-12 cells were plated in collagen-coated, 60 mm plastic petri dishes with or without 5 ng/ml NGF and were exposed to sinusoidal magnetic fields for 22 h in a CO2 incubator at 37 degrees C. One 1,000-turn coil, 20 cm in diameter, generated vertically oriented magnetic fields. The dishes were stacked on the center axis of the coil to provide a range of intensities between 3.5 and 9.0 microT rms. The flux density of the ambient DC magnetic field was 37 microT vertical and 19 microT horizontal. The assay consisted of counting over 100 cells in the central portion (radius < or = 0.3 cm) of each dish and scoring cells positive for NO. Sham exposure of cells treated identically with NGF demonstrated no difference in the percentage of cells with NO between exposed and magnetically shielded locations within the incubator. Analysis of variance demonstrated flux density-dependent reductions in NGF-stimulated NO over the 35-70 Hz frequency range, whereas frequencies between 15 Hz and 30 Hz produced no obvious reduction. The results also demonstrated a relative maximal sensitivity of cells at 40 Hz with a possible additional sensitivity region at or above 70 Hz. These findings suggest a biological influence of perpendicular AC/DC magnetic fields different from those identified by the ion parametric resonance model, which uses strictly parallel AC/DC fields.
authors.affiliation: Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: Blackman
authors.forename: S G
authors.initials: SG
authors.lastname: Benane
authors.forename: D E
authors.initials: DE
authors.lastname: House
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D008280:Magnetics / Q000009:adverse effects*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000648:ultrastructure*; D016716:PC12 Cells; D051381:Rats
chemical_list: D009414:Nerve Growth Factors