title: Construction and use of a new vector/transposon, pLBT::mini-Tn10:lac:kan, to identify environmentally responsive genes in a marine bacterium.
abstract: The previously described pLOFKm transposon delivery plasmid (J.Bacteriol. (1990) 172, 6557-6567) was engineered such that a promotorless lacZ gene was cloned within the transposon cassette, generating the vector pLBT. Using pLBT, stable insertion mutations were generated at high frequencies in Vibrio sp. S141 and Pseudomonas sp. S91, and the interrupted genes could be monitored for their pattern of regulation. Genetic screens isolated mutants defective in a variety of activities. We describe the construction and use of pLBT as a tool for reporter gene mutant analysis in bacteria other than well-characterized laboratory strains.
authors.affiliation: Department of General and Marine Microbiology, Lundberg Laboratory, University of Göteborg, Sweden.
authors.forename: N H
authors.initials: NH
authors.lastname: Albertson
authors.forename: S
authors.initials: S
authors.lastname: Stretton
authors.forename: S
authors.initials: S
authors.lastname: Pongpattanakitshote
authors.forename: J
authors.initials: J
authors.lastname: Ostling
authors.forename: K C
authors.initials: KC
authors.lastname: Marshall
authors.forename: A E
authors.initials: AE
authors.lastname: Goodman
authors.forename: S
authors.initials: S
authors.lastname: Kjelleberg
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002245:Carbon Dioxide / Q000494:pharmacology; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D007763:Lac Operon; D008386:Marine Biology; D016254:Mutagenesis, Insertional; D011549:Pseudomonas / Q000235:genetics; D014733:Vibrio / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D002245:Carbon Dioxide