title: DNA sequencing by capillary electrophoresis using short oligonucleotide primer libraries.
abstract: Two strategies for DNA sequencing by primer walking using short oligonucleotide primer libraries have been successfully employed along with capillary electrophoresis using replaceable polymer solutions of linear polyacrylamide and fluorescence detection. A 3.5-kb stretch of the single-stranded M13mp18 template was sequenced with T7 PRISM dye-terminator/Sequenase chemistry. An in-house base-calling program offered read lengths of roughly 450 bases with an average of 97.8% accuracy.
authors.affiliation: Northeastern University, Boston, MA, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Ruiz-Martinez
authors.forename: E
authors.initials: E
authors.lastname: Carrilho
authors.forename: J
authors.initials: J
authors.lastname: Berka
authors.forename: J
authors.initials: J
authors.lastname: Kieleczawa
authors.forename: A W
authors.initials: AW
authors.lastname: Miller
authors.forename: F
authors.initials: F
authors.lastname: Foret
authors.forename: S
authors.initials: S
authors.lastname: Carson
authors.forename: B L
authors.initials: BL
authors.lastname: Karger
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers