title: Affinity-purified Dermatophagoides farinae antigen induces CD23 on T and B lymphocytes and monocytes specifically in patients with atopic dermatitis.
abstract: The purpose of this work is to analyze CD23 expression on subpopulations of peripheral blood mononuclear cells (PBMC) from the patients with atopic dermatitis (AD) after stimulation with affinity-purified Dermatophagoides farinae antigen (Af-DF). PBMC from mite-allergic AD patients stimulated with Af-DF induced striking expression of CD23 after the culture. The frequencies of CD23-positive (CD23+) PBMC in the culture were significantly higher than those without stimulation. Most of the CD23+ cells belonged to B lymphocytes and monocytes subsets. CD23 was induced in less than 1% of CD3+, CD4+ and CD8+ lymphocytes by Af-DF. However, significant differences were observed compared to the spontaneous expression, and dose-dependencies were confirmed. No significant increase in the frequencies of CD23+ PBMC was found in non-mite-allergic AD patients and non-atopic donors after stimulation with Af-DF. The kinetics of CD23 expression by Af-DF showed similar patterns to that by IL-4 stimulation. Our results demonstrate that Af-DF can induce CD23 expression on PBMC in mite-allergic AD patients. Based on the high titer of mite-specific IgE antibody in blood of AD, mite antigen may, at least in part, contribute to high CD23 expression in AD.
authors.affiliation: Department of Dermatology, Nagasaki University School of Medicine, Japan.
authors.forename: H M
authors.initials: HM
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: S
authors.initials: S
authors.lastname: Anan
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002846:Chromatography, Affinity; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008925:Mites / Q000276:immunology*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D017455:Receptors, IgE / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D017455:Receptors, IgE