Identification of CYP2B14P and CYP2B16P, two apparent pseudogenes in the rat cytochrome P450 2B (CYP2B) subfamily.
Cytochrome P450 2B3 (CYP2B3) is a member of the CYP2B subfamily and is present constitutively in rat liver. During cloning of the CYP2B3 gene, several variant genomic inserts were isolated, exoncontaining fragments of which were identified by hybridizing to CYP2B3 cDNA or exonic probes. Nucleotide sequence analysis of one set of variant inserts showed them to carry exon 1 and the 5'-flanking region of a gene designated CYP2B14P. The last codon of CYP2B14P exon 1 is a TAG translation stop codon, and thus CYP2B14P is a pseudogene. Three other inserts covering 34 kb of genomic sequence together carried 9 exons and the 5'-and 3'-flanking regions of the gene designated CYP2B16P. The 5'-splice site of CYP2B16P intron 1 is inactivated by the replacement of G by T in the normally invariant GT dinucleotide. Thus, CYP2B16P also has the characteristics of a pseudogene. The identification of CYP2B14P and CYP2B16P brings to at least seven the number of genes or pseudogenes shown by nucleotide sequence analysis to belong to the rat CYP2B subfamily.
Trottier
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Centre de recherche en cancérologie, Université Laval, centre Hospitalier de Québec, Canada.
Dubois
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S
Jean
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Anderson
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D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D000072467:Cytochrome P450 Family 2; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D011544:Pseudogenes / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping
D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C089142:Cyp2b3 protein, rat; D000072467:Cytochrome P450 Family 2