[Turner's syndrome and isoforms of LH and FSH. Value of polyclonal enzymatic techniques].
BACKGROUND
The glycoproteic hormones, LH and FSH, circulate under heterogenous molecular forms, the isoforms. The more acidic isoforms of FSH are found in hypogonadic patients and a displacement towards the basic forms is observed during substitutive treatment.


METHODS
A 13 year-old girl with Turner syndrome was examined for pubertal delay. Ultrasonography failed to see both ovaries and endocrine investigations showed a type P1 response (LH-RH test using immunological method). Control of hormonal levels by a polyclonal immunoenzymatic method confirmed primary hypogonadism.


CONCLUSIONS
Radioimmunological methods using monoclonal antibodies can underevaluate FSH and LH levels under circumstances in which the distribution of isoforms may vary. Discrepancy must lead to the measure of gonadotrophins using polyclonal immunoenzymatic methods.
Petrus
M
M
Service de pédiatrie, CHU, Tarbes, France.
Rittie
J L
JL
Causse
J E
JE
Moulie
N
N
Rhabbour
M
M
Netter
J C
JC
Hirsch
C
C
Bildstein
G
G
D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D007006:Hypogonadism / Q000097:blood; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry*; D014424:Turner Syndrome / Q000097:blood*
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
Syndrome de Turner et isoformes de LH et FSH. Intérêt des techniques enzymatiques polyclonales.