Characterization of a novel anti-inflammatory factor produced by RM3/1 macrophages derived from glucocorticoid treated human monocytes.
Glucocorticoids exert their anti-inflammatory activity by modulating the functions of various cell types including macrophages. They also induce the generation of a distinct macrophage subtype defined by the surface antigen RM3/1 which appears to be associated with the down-regulation of inflammation. Supernatants from these cells were found to exert a dose-dependent anti-inflammatory effect, particularly in the early phase as shown in the 5-hydroxytryptamine (5-HT) induced footpad edema of mice. By using conventional purification methods the anti-inflammatory factor was found to have a molecular mass of about 78 kD and an isoelectric point of about 7.9. Heat lability and sensitivity to trypsin and proteinase K indicate the protein nature of the anti-inflammatory factor. The inhibition of the early phase of inflammation and the molecular weight suggest that the anti-inflammatory agent released from RM3/1 macrophages is a novel protein different from other anti-inflammatory proteins described so far.
Hamann
W
W
Medical Faculty, Institute of Pharmacology and Toxicology, Germany.
Flöter
A
A
Schmutzler
W
W
Zwadlo-Klarwasser
G
G
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D002351:Carrageenan; D002460:Cell Line; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D007525:Isoelectric Focusing; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D012701:Serotonin; D002351:Carrageenan