title: Kv beta 1 subunit binding specific for shaker-related potassium channel alpha subunits.
abstract: Voltage-activated potassium (Kv) channels from mammalian brain are hetero-oligomers containing alpha and beta subunits. Coexpression of Kv1 alpha and Kv beta 1 subunits confers rapid A-type inactivation on noninactivating potassium channels (delayed rectifiers) in expression systems in vitro. We have delineated a Kv1.5 aminoterminal region of up to 90 amino acids (residues 112-201) that is sufficient for interactions of Kv1.5 alpha and Kv beta 1 subunits. Within this region of the Kv1.5 amino terminus (residues 193-201), a Kv beta 1 interaction site necessary for Kv beta 1-mediated rapid inactivation of Kv1.5 currents was detected. This interaction site motif (FYE/QLGE/DEAM/L) is found exclusively in the Shaker-related subfamily (Kv1). The results show that hetero-oligomerization between alpha and Kv beta 1 subunits is restricted to Shaker-related potassium channel alpha subunits.
authors.affiliation: Zentrum für Molekulare Neurobiologie Institut für Neurale Signalverarbeitung, Hamburg Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Sewing
authors.forename: J
authors.initials: J
authors.lastname: Roeper
authors.forename: O
authors.initials: O
authors.lastname: Pongs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D017124:Conserved Sequence; D004330:Drosophila / Q000235:genetics*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D015221:Potassium Channels