Binding of human GM-CSF to synthetic peptides of the alpha subunit of its receptor.
We have synthesized a series of peptides corresponding to portions of the extracellular domain of human granulocyte-macrophage colony stimulating factor receptor alpha subunit (hGM-CSFR alpha). The sequences were chosen according to the homology between hGM-CSFR alpha and the growth hormone receptor (GHR) and correspond to the regions reported to form the binding site of the latter receptor. The peptides were examined for their binding activity to hGM-CSF by affinity chromatography on resin-immobilized hGM-CSF and by a solid phase binding assay. For peptides endowed with hGM-CSF binding activity were identified and the postulated homology between the binding sites of hGM-CSFR alpha GHR was confirmed.
Di Bartolo
V
V
Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.
Danè
A
A
Cassano
E
E
Viganò
S
S
Chiello
E
E
Verniani
D
D
Beffy
P
P
Pegoraro
S
S
Hamdan
M
M
Rovero
P
P
Revoltella
R P
RP
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000737:chemistry; D016415:Sequence Alignment
D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D011986:Receptors, Somatotropin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor