title: [Practical aspects of leukotriene E4 determination in urine].
abstract: Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and radioimmunological capabilities. The recent availability of kits for immunoenzymatic assay of leukotrienes offers a new tool for clinical laboratories provided the limitations of the method are understood. We suggest a simplified methodology for direct urinary LTE4 detection and outline a number of areas of concern encountered with this method.
authors.affiliation: Centre d'allergie, hôpital Rothschild, Paris, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Chabane
authors.forename: V
authors.initials: V
authors.lastname: Le Vraux
authors.forename: A M
authors.initials: AM
authors.lastname: Le Provost
authors.forename: F
authors.initials: F
authors.lastname: Leynadier
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology / Q000652:urine; D004342:Drug Hypersensitivity / Q000652:urine; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017999:Leukotriene E4 / Q000652:urine*; D008297:Male; D014862:Wasp Venoms / Q000009:adverse effects
chemical_list: D000485:Allergens; D014862:Wasp Venoms; D017999:Leukotriene E4
vernacular_title: Aspects pratiques du dosage du leucotriène E4 dans les urines.