title: Reversible interruption of gap junctional communication by testosterone propionate in cultured Sertoli cells and cardiac myocytes.
abstract: A direct cell-to-cell exchange of ions and molecules occurs through specialized membrane channels built by the interaction of two half channels, termed connexons, contributed by each of the two adjacent cells. The electrical and diffusional couplings have been investigated by monitoring respectively the cell-to-cell conductance and the fluorescence recovery after photobleaching, in Sertoli and cardiac cells of young rat. In both cell types, a rapid impairment of the intercellular coupling has been observed in the presence of testosterone propionate. This interruption of the cell-to-cell communication through gap junction channels was dose-dependent, observed in the concentration range 1 to 25 microM and was progressively reversed after withdrawing the testosterone ester. Pretreatment with cyproterone acetate, an antiandrogen which blocks the nuclear testosterone receptor by binding, did not prevent the uncoupling action of the androgen ester. This observation, together with the rapid time course of the uncoupling and recoupling, and the rather high effective concentration (micromolar) of the steroid compound, suggests a nongenomic mechanism of action. The uncoupling concentrations were very similar to those of other steroid compounds known to interrupt gap junctional communication. The uncoupling could result from a direct interaction of the steroid with the proteolipidic structure of the membrane, that might alter the conformation of the gap junction channels and their functional state.
authors.affiliation: Laboratoires de Physiologie Cellulaire et de Physiologie Animale, Unité de Recherche Associée au CNRS, Poitiers, France.
authors.forename: F
authors.initials: F
authors.lastname: Pluciennik
authors.forename: F
authors.initials: F
authors.lastname: Verrecchia
authors.forename: B
authors.initials: B
authors.lastname: Bastide
authors.forename: J C
authors.initials: JC
authors.lastname: Hervé
authors.forename: M
authors.initials: M
authors.lastname: Joffre
authors.forename: J
authors.initials: J
authors.lastname: Délèze
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002478:Cells, Cultured; D017629:Gap Junctions / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000166:cytology; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone