Expression of glucagon-like peptide 1 receptor in a murine C cell line: regulation of calcitonin gene by glucagon-like peptide 1.
We have characterized, by RT-PCR amplification using specific primers, the presence of glucagon-like peptide-1 (GLP-I) receptor mRNA in CA-77 cells, a C cell line derived from a rat medullary thyroid carcinoma. Down-regulation of the GLP-1 receptor mRNA was observed after exposure of CA-77 C cells with GLP-1 (7-37). Increased secretion of both calcitonin gene-related peptide (CGRP) and calcitonin (CT) occurred after treatment with GLP-1 (7-37) associated with elevated steady-state levels of CGRP and CT mRNA. GLP-1 (7-37) increased cAMP formation in CA-77 cells in a dose-dependent manner; exendin (9-39), a GLP-1 receptor antagonist, inhibited cAMP production. The GLP-1 peptide which is produced by intestinal cells could be involved in the control of CT secretion through an entero-thyroidal axis implying GLP-1 receptor and increased CT gene expression.
Lamari
Y
Y
E.A. 1526 DRED, Université Pierre et Marie Curie, Paris, France.
Boissard
C
C
Moukhtar
M S
MS
Jullienne
A
A
Rosselin
G
G
Garel
J M
JM
D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000096:biosynthesis*; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002460:Cell Line; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005934:Glucagon / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D000067757:Glucagon-Like Peptide-1 Receptor; D004763:Glucagon-Like Peptides / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D010455:Peptides / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018027:Receptors, Glucagon / Q000096:biosynthesis*; D013964:Thyroid Neoplasms; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D017931:DNA Primers; C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D010455:Peptides; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D002116:Calcitonin; D005934:Glucagon; D015740:Calcitonin Gene-Related Peptide; C051322:glucagon-like peptide 1 (7-37)