Sodium fluoride inhibits the antisecretory effect of peptide YY and its analog in rabbit jejunum.
The antisecretory peptide YY (PYY) inhibits jejunal secretion through and inhibitory protein (Gi), whereas sodium fluoride (NaF) is a potent activator of G-proteins. This work was conducted to characterize the role of NaF in the antisecretory effect of PYY. For this purpose, electrogenic chloride secretion was assessed by measuring the in vitro variations in short-circuit current (delta Isc) due to alterations in ionic transport, using Ussing chambers Results: 1) NaF induced a transient increase in Isc at concentrations exceeding 5 mM. 2) 2 mM NaF inhibited the antisecretory effect of 0.1 microM PYY and of its analog P915. 3) stimulation of secretion by forskolin and dbcAMP was halved in the presence of 2 mM NaF. 4) Inhibition of protein kinase C by 0.1 mM bisindolylmaleimide caused a sustained increase in Isc in the presence of 5 mM NaF. In conclusion, these results confirm that PYY inhibits electrogenic chloride secretion and show that NaF stimulates it, and suggest that NaF reduces PYY-induced inhibition via a G-dependent and a G-independent functional pathway.
Eto
B
B
Unité de Recherche sur les Fonctions Intestinales, le Métabolisme et la Nutrition, Hôpital Saint-Lazare, Paris, France.
Boisset
M
M
Desjeux
J F
JF
D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors; D005576:Colforsin / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000187:drug effects*; D005768:Gastrointestinal Hormones / Q000037:antagonists & inhibitors*; D007211:Indoles / Q000494:pharmacology; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008301:Maleimides / Q000494:pharmacology; D019894:Peptide YY; D010455:Peptides / Q000037:antagonists & inhibitors*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011817:Rabbits; D012636:Secretory Rate / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D005768:Gastrointestinal Hormones; D007211:Indoles; D008301:Maleimides; D010455:Peptides; D019894:Peptide YY; D005576:Colforsin; D003994:Bucladesine; D012969:Sodium Fluoride; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; C070515:bisindolylmaleimide I