Prevalence of enterohemorrhagic Escherichia coli in raw and treated municipal sewage.
Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a key protein involved in the virulence of enterohemorrhagic Escherichia coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II was present in a single sample of untreated sewage and absent in all other samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n = 1,520) also tested negative for SLT II by colony hybridization.
Grant
S B
SB
Department of Civil and Environmental Engineering, University of California, Irvine 92717, USA. sbgrant@uci.edu
Pendroy
C P
CP
Mayer
C L
CL
Bellin
J K
JK
Palmer
C J
CJ
D001427:Bacterial Toxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D006471:Gastrointestinal Hemorrhage / Q000382:microbiology*; D012722:Sewage; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014871:Water Microbiology
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D012722:Sewage; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2