title: CpG methylation directly inhibits binding of the human papillomavirus type 16 E2 protein to specific DNA sequences.
abstract: CpG methylation of the human papillomavirus upstream regulatory region has previously been shown to reduce virus promoter activity. Here, we demonstrate that methylation of the CpG dinucleotides contained within the binding site of the human papillomavirus type 16 E2 protein has a direct effect on the interaction of this protein with DNA. Methylation of both CpG dinucleotides within the E2 site abolishes the binding of E2.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Thain
authors.forename: O
authors.initials: O
authors.lastname: Jenkins
authors.forename: A R
authors.initials: AR
authors.lastname: Clarke
authors.forename: K
authors.initials: K
authors.lastname: Gaston
mesh_terms: D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins; D006801:Humans; D008745:Methylation; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral