Characterization and replication mode determination of the minimal replicon of Tetragenococcus halophila ATCC33315 plasmid pUCL287.
pUCL287 is a cryptic plasmid of Tetragenococcus halophila (formerly Pediococcus halophilus) ATCC33315 of relatively small size (8.7 kb). Its minimal replicon was located on a 1235 bp MamI-EcoRI fragment. This minimal replicon contains a non-translated region, followed by a gene encoding a putative 311 amino acid protein. Deletion experiments showed that the non-translated region corresponds to the replication origin. Determination of the replication mode was carried out in Enterococcus faecalis JH2-2 harboring pUCL287 minimal replicon. The replicating intermediates detected revealed that pUCL287 minimal replicon follows a bidirectional theta replicating mode.
Benachour
A
A
Laboratoire de Microbiologie de l'Environnement, Université de Caen, France.
Frère
J
J
Boutibonnes
P
P
Auffray
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010376:Pediococcus / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D012093:Replicon / Q000235:genetics*
D004269:DNA, Bacterial