title: Nitric oxide inhibitors attenuate ischemic degeneration in the CA1 region of rat hippocampal slices.
abstract: We examined the involvement of nitric oxide (NO) in ischemic brain damage using hippocampal slices prepared from 30 day old albino rats and exposed to 20 min of oxygen/glucose deprivation (ischemia) followed by 90 min postincubation in oxygen- and glucose-containing media. Damage in the CA1 region was rated on a 0 (intact) to 4 (severe neuronal damage) scale by a rater blind to the experimental condition. Control slices exposed to ischemia were rated as 2.8 +/- 0.4 (N = 12). L-NG-Monomethylarginine (100 microM) and L-NG-nitroarginine (100 microM), non-selective NO synthase (NOS) inhibitors, diminished ischemic damage (0.6 +/- 0.3, N = 8, and 1.0 +/- 0.5, N = 4, respectively). An inhibitor of brain NOS, 7-nitroindazole (30 microM), was also effective against ischemic degeneration (0.7 +/- 0.3, N = 5). These results suggest that activation of NOS is involved in ischemic degeneration in the CA1 region.
authors.affiliation: Department of Psychiatry, Washington University, School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
authors.forename: A M
authors.initials: AM
authors.lastname: Benz
authors.forename: D B
authors.initials: DB
authors.lastname: Clifford
authors.forename: C F
authors.initials: CF
authors.lastname: Zorumski
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005947:Glucose / Q000502:physiology; D006624:Hippocampus / Q000473:pathology*; D002534:Hypoxia, Brain / Q000473:pathology; D007191:Indazoles / Q000494:pharmacology; D016202:N-Methylaspartate / Q000502:physiology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D019323:omega-N-Methylarginine / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D007191:Indazoles; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D016202:N-Methylaspartate; D019001:Nitric Oxide Synthase; D005947:Glucose; C080122:7-nitroindazole