title: Very late antigen (VLA) expression by normal and neoplastic human plasma cells; including an assessment of antibodies submitted to the Vth International Workshop on Leucocyte Differentiation Antigens using human myeloma cell lines.
abstract: The biology of normal plasma cells and the pathophysiology of human multiple myeloma remain poorly understood. Functional assays are scarce and at present cell phenotyping is providing the most information about how human plasma cells may behave. Three different types of human plasma cells: normal, fresh neoplastic myeloma cells and plasma cell lines, have been studied for their reactivity with antibodies to the beta-1 integrins (Very Late Antigens; VLAs), including a panel obtained from the Vth International Workshop on Leucocyte Differentiation Antigens. Most plasma cell targets express VLA-4 (CD49d positive) and the common beta chain recognized by CD29. CD49e (VLA-5) was occasionally positive. Other VLAs were not usually expressed. These data suggest the wide use by plasma cells of VLA-4, possibly as a ligand with fibronectin and high endothelial venules (HEV). Of other adhesion structures expressed by plasma cells, only CD44 is seen as frequently, and this is also a HEV ligand.
authors.affiliation: Department of Immunology, Medical School, Birmingham, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Drew
authors.forename: H F
authors.initials: HF
authors.lastname: Barker
authors.forename: J
authors.initials: J
authors.lastname: Ball
authors.forename: C
authors.initials: C
authors.lastname: Pearson
authors.forename: G
authors.initials: G
authors.lastname: Cook
authors.forename: I
authors.initials: I
authors.lastname: Franklin
mesh_terms: D000906:Antibodies; D000918:Antibody Specificity; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D001854:Bone Marrow Cells; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D039041:Integrin alpha4beta1; D019012:Integrin beta1 / Q000032:analysis / Q000096:biosynthesis; D016023:Integrins / Q000032:analysis / Q000096:biosynthesis*; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology; D010950:Plasma Cells / Q000276:immunology*; D017469:Receptors, Fibronectin / Q000032:analysis / Q000096:biosynthesis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D039041:Integrin alpha4beta1; D019012:Integrin beta1; D016023:Integrins; D017469:Receptors, Fibronectin; D016201:Receptors, Lymphocyte Homing