title: Interaction of Daunomycin with Dipalmitoylphosphatidylcholine Model Membranes. A 1H NMR Study
abstract: 1H NMR parameters were obtained for daunomycin in water solution in the free state as well as in the presence of dipalmitoylphosphatidylcholine model membranes. Spin-lattice relaxation rates were measured under nonselective, single-selective, and double-selective irradiation modes, and 2D NOESY spectra were obtained at several values of the mixing time. Proton-proton distances were calculated and the motional correlation time was evaluated in both the free and bound states. NMR parameters were used to show that ring A and the glucosamine moiety of daunomycin strongly interact with the external surface of the bilayer, while the rest of the molecule penetrates the membrane without crossing it. The structures of both free and bound daunomycin were obtained and compared by using molecular modeling.
authors.affiliation: Department of Chemistry, University of Siena, Pian dei Mantellini 44, Siena, 53100, Italy
authors.initials: L
authors.lastname: Calzolai
authors.initials: E
authors.lastname: Gaggelli
authors.initials: A
authors.lastname: Maccotta
authors.initials: G
authors.lastname: Valensin