[The use of the polymerase chain reaction for the identification of Clostridium botulinum type A strains].
Polymerase chain reaction (PCR) for the identification of C.botulinum of type A was developed. As primers, oligonucleotides corresponding to sequences 913 -- 932 and 1852 -- 1871 of the gene of type A botulinic neurotoxin were used. The study revealed that under optimum conditions the positive result of the reaction was registered only when the DNA of C.botulinum strains of type A (11 strains) was used, but not that of C.botulinum strains of other types (11 strains of type B, 5 strains of type C, 2 strains of type D, 6 strains of type E and 1 strain of type G). High sensitivity, specificity and rapidity of PCR open good prospects for its practical use.
Shliapnikova
O V
OV
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Efimenko
A N
AN
Bulatova
T I
TI
Liaĭman
M E
ME
Vertiev
Y V
YV
D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics; D003014:Clostridium botulinum / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D001905:Botulinum Toxins