title: Role of proximal promoter elements in regulation of renin gene transcription.
abstract: Mouse As4.1 cells, obtained after transgene-targeted oncogenesis to induce neoplasia in renal renin-expressing cells, express high levels of renin mRNA from the endogenous Ren-1(c) gene. We have used these cells to characterize the role of the Ren-1(c) proximal promoter (+6 to -117) in the regulation of renin gene transcription. It was found that 4.1 kilobases (kb) of Ren-1(c) 5'-flanking sequence, in combination with the proximal promoter, are required for strong activation (approximately 2 orders of magnitude over the basal level of the promoter alone) of the chloramphenicol acetyltransferase reporter in transfection assays. Within the 4.1-kb fragment, a 241-base pair region was identified that retains full activity in an orientation-independent manner in combination with the promoter. The resulting transcripts initiate at the normal renin start site. Electrophoretic mobility shift assays identified a sequence at approximately position -60 in the promoter region that binds nuclear proteins specific for renin-expressing As4.1 cells. Mutations in this sequence, which disrupt binding of nuclear protein(s), completely abolish activation of transcription by the 4. 1-kb fragment. Activation of transcription by the 241-base pair enhancer was still observed, although it was diminished in magnitude (60-fold over the mutated promoter alone). We present a model derived from the current data that suggests that regulation of renin expression is achieved through cooperation of transcription factors binding at the proximal promoter element and a distal enhancer element to abrogate or override the effects of an intervening negative regulatory region.
authors.affiliation: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
authors.forename: N
authors.initials: N
authors.lastname: Petrovic
authors.forename: T A
authors.initials: TA
authors.lastname: Black
authors.forename: J R
authors.initials: JR
authors.lastname: Fabian
authors.forename: C
authors.initials: C
authors.lastname: Kane
authors.forename: C A
authors.initials: CA
authors.lastname: Jones
authors.forename: J A
authors.initials: JA
authors.lastname: Loudon
authors.forename: J P
authors.initials: JP
authors.lastname: Abonia
authors.forename: C D
authors.initials: CD
authors.lastname: Sigmund
authors.forename: K W
authors.initials: KW
authors.lastname: Gross
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012083:Renin / Q000235:genetics*; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D009687:Nuclear Proteins; D012083:Renin