title: Improved isolation of genomic DNA from mycobacteria in agarose plugs by rapid lysis with a combination of N-acetylglucosaminidase and lysozyme.
authors.affiliation: Dong-Guk University Kyungpook, Korean Institute of Tuberculosis, Seoul, South Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Choe
authors.forename: Y J
authors.initials: YJ
authors.lastname: Huh
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: J R
authors.initials: JR
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: J C
authors.initials: JC
authors.lastname: Song
authors.forename: J H
authors.initials: JH
authors.lastname: Ko
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
authors.forename: O
authors.initials: O
authors.lastname: Nashiru
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: G H
authors.initials: GH
authors.lastname: Bai
authors.forename: T H
authors.initials: TH
authors.lastname: Chung
authors.forename: T W
authors.initials: TW
authors.lastname: Chung
authors.forename: I S
authors.initials: IS
authors.lastname: Choe
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000118:Acetylglucosaminidase; D001433:Bacteriolysis; D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D006358:Hot Temperature; D009113:Muramidase; D009161:Mycobacterium / Q000235:genetics*; D009162:Mycobacterium avium / Q000235:genetics; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics; D009163:Mycobacterium bovis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D012685:Sepharose; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D012685:Sepharose; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific; D009113:Muramidase; D000118:Acetylglucosaminidase