Regulation by protein kinase C of transforming growth factor-beta 1 action on the proliferation of vascular smooth muscle cells from spontaneously hypertensive rats.
1. This study examined the role of protein kinase C (PKC) on the action of transforming growth factor-beta 1 (TGF-beta 1) to regulate the proliferation of vascular smooth muscle cells (VSMC) isolated from the aorta of the spontaneously hypertensive rat (SHR). 2. Down-regulation of PKC by prolonged exposure to phorbol 12-myristate 13-acetate (PMA) completely inhibited the ability of TGF-beta 1 to potentiate epidermal growth factor-stimulated proliferation of VSMC. 3. In contrast, the inhibitory effect of TGF-beta 1 on serum-stimulated proliferation of VSMC was not altered by PMA action. 4. These results suggest that PKC-dependent signalling pathways involved in the regulation of growth by TGF-beta 1 may be important in any proliferative component of vascular hypertrophy that develops in the SHR.
Saltis
J
J
Baker Medical Research Institute, Alfred Hospital, Prahran, Victoria, Australia.
Bobik
A
A
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000502:physiology*
D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate