In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-resonance NMR.
Rotational-echo double-resonance (REDOR) 13C NMR spectra with 2H dephasing have been obtained from plaques and threads from the byssus of the marine mussel Mytilus edulis labeled by sea-water exposure to L-[ring-4-(13)C]tyrosine and L-[ring-d4]tyrosine for 2 days. Specific isotopic enrichment of tyrosine in protein reached 25% in both 13C and 2H. Fifteen percent of the total 13C label was incorporated as diphenolic carbon. Based on REDOR dephasing, about one-tenth of tyrosine rings in both intact plaques and threads are within 4 A of each other or rings of 3,4-dihydroxy-phenylalanine (DOPA). However, there is no direct evidence for the formation of covalent linkages between or among tyrosine and DOPA rings in either plaques or threads.
Klug
C A
CA
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
Burzio
L A
LA
Waite
J H
JH
Schaefer
J
J
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002247:Carbon Isotopes; D003432:Cross-Linking Reagents; D003903:Deuterium; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry
D002247:Carbon Isotopes; D003432:Cross-Linking Reagents; D010455:Peptides; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D003903:Deuterium