The role of physicochemical properties of biomaterials and bacterial cell adhesion in vitro.
This study was undertaken to investigate the physicochemical aspects of the interaction between the surface of biomaterials and bacterial cell membranes in vitro, aimed at studying the mechanisms of bacterial adhesion to biomaterials. Correlations were made between the number of adherent bacterial cells (S. aureus) and each of the calculated components of surface free energy (i.e., dispersion, polarity and hydrogen bond) of biomaterials. The effect of antibodies to cell-adhesion molecules on bacterial adhesion was also studied using monoclonal antibodies to vitronectin receptor, fibronectin receptor and CD44. This study indicates the polarity component of surface free energy plays a dominant role in the process of bacterial adhesion at least in vitro. The number of cells adherent to materials decreased to 44-73% of the control value in the presence of antibodies tested, showing that cell adhesion molecules affect adherence to biomaterials. Moreover, the results suggested that bacterial adhesion was prevented by specific blockade of cell adhesion molecule receptors.
Kitano
T
T
Department of Orthopaedic Surgery, Osaka City University Medical School, Japan.
Yutani
Y
Y
Shimazu
A
A
Yano
I
I
Ohashi
H
H
Yamano
Y
Y
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001419:Bacteria / Q000166:cytology*; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000276:immunology; D002452:Cell Count; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D017469:Receptors, Fibronectin / Q000276:immunology / Q000378:metabolism; D019030:Receptors, Vitronectin / Q000276:immunology / Q000378:metabolism; D013211:Staphylococcus aureus / Q000166:cytology / Q000378:metabolism; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D017469:Receptors, Fibronectin; D019030:Receptors, Vitronectin