title: T helper type-1 (Th1)/Th2 profiles of peripheral blood mononuclear cells (PBMC); responses to antigens of Chlamydia trachomatis in subjects with severe trachomatous scarring.
abstract: Increased stimulation of Th2 cytokines may contribute to the development of persistent ocular chlamydial infection, resulting in the blinding pathological changes of trachoma. Proliferation and cytokine production profiles of PBMC in response to stimulation with antigens of Chlamydia trachomatis were compared in 30 patients with severe conjunctival scarring due to trachoma and in 30 age-, sex- and location-matched controls. Interferon-gamma (IFN-gamma) and IL-4 were detected at the single-cell level by ELISPOT assay. Transcription of the genes encoding IFN-gamma, IL-4 and IL-10 was detected in mRNA isolated from parallel cultures of PBMC using reverse transcriptase-polymerase chain reaction (RT-PCR). Incubation with the chlamydial heat shock protein (hsp)60 resulted in increased numbers of IL-4-producing cells in PBMC isolated from patients with scarring disease and increased secretion of IFN-gamma from PBMC of control subjects. Incubation with the chlamydial major outer membrane protein (MOMP) increased the number of IFN-gamma-producing cells in the control group only. Messenger RNA encoding IL-4 was only detected in PBMC of patients with scarring disease after in vitro stimulation with chlamydial antigens, but IFN-gamma mRNA and IL-10 mRNA were also more frequently detected in this group. Thirty-eight subjects were HLA-DRB1 and -DQB1 typed. Associations were observed between certain HLA class II alleles and cellular immune responses to chlamydial antigens. No HLA associations were found with clinical status, and overall we found no evidence of strong associations and the type of immune response. These data are consistent with a role for Th2 cells and cytokines in the pathogenesis of trachomatous scarring.
authors.affiliation: Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Holland
authors.forename: R L
authors.initials: RL
authors.lastname: Bailey
authors.forename: D J
authors.initials: DJ
authors.lastname: Conway
authors.forename: F
authors.initials: F
authors.lastname: Culley
authors.forename: G
authors.initials: G
authors.lastname: Miranpuri
authors.forename: G I
authors.initials: GI
authors.lastname: Byrne
authors.forename: H C
authors.initials: HC
authors.lastname: Whittle
authors.forename: D C
authors.initials: DC
authors.lastname: Mabey
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D002648:Child; D002692:Chlamydia trachomatis / Q000276:immunology*; D002921:Cicatrix / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D006683:HLA-DQ Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism; D014141:Trachoma / Q000097:blood / Q000276:immunology* / Q000473:pathology; D014158:Transcription, Genetic / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015847:Interleukin-4; D007371:Interferon-gamma