Amelioration of hepatocellular integrity and inhibition of sinusoidal oxidative stress by N-acetylcysteine pretreatment in cold ischemia-reperfusion injury of rat liver.
Further improvements of donor liver preservation are still required in liver transplantation. In the present study, we investigated whether intraportal injection of N-acetylcysteine (NAC) 15 min before flush-out of UW solution (NAC pretreatment) improves liver dysfunction after cold preservation or has a protective effect on sinusoidal oxidative stress. The effect of NAC pretreatment was examined using an isolated perfused rat liver model. The NAC pretreatment significantly reduced sinusoidal oxidative stress relative to a control dextrose 5% injection. Under a glutathione-depleted condition produced by L-buthionine-[S-R]-sulfoximine, the NAC pretreatment also significantly reduced hepatocellular as well as sinusoidal oxidative stress, resulting in improvement of hepatocelllar integrity relative to a control dextrose 5% injection.
Nakano
H
H
Laboratoire de Chirurgie Expérimentale de la Fondation Transplantation, Université Louis-Pasteur, Hôpital Universitaire de Hautepierre, Strasbourg, France.
Boudjema
K
K
Jaeck
D
D
Alexandre
E
E
Imbs
P
P
Chenard
M P
MP
Nagasaki
H
H
Kumada
K
K
Wolf
P
P
Cinqualbre
J
J
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
D019328:Buthionine Sulfoximine; D005978:Glutathione; D000111:Acetylcysteine