The structure of the O-specific polysaccharide of Proteus penneri 52.
An acidic O-specific polysaccharide isolated from Proteus penneri 52 contains D-galacturonic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galactose in the ratio 1:2:2. On the basis of sugar analysis and NMR spectroscopy, which included one-dimensional TOCSY, two-dimensional COSY, heteronuclear 13C, 1H-COSY, and rotating-frame NOE spectroscopy, the following structure of the pentasaccharide repeating unit of the O-specific polysaccharide was established: [sequence: see text] Cross-reactivity of the anti-P-penneri 52 O-serum with other strains of P. penneri isolated in Germany. Poland, USA, and Canada is discussed and a new Proteus serogroup is proposed.
Sidorczyk
Z
Z
Institute of Microbiology and Immunology, University of Lódź, Poland.
Zych
K
K
Swierzko
A
A
Vinogradov
E V
EV
Knirel
Y A
YA
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D007106:Immune Sera; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011511:Proteus / Q000737:chemistry / Q000254:growth & development / Q000276:immunology*
D007106:Immune Sera; D019081:O Antigens; D009844:Oligosaccharides