Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery.
The influence of sonication power, suspension volume and cell concentration on the kinectics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration.
Santos
J A
JA
Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa, Portugal.
Belo
I
I
Mota
M
M
Cabral
J M
JM
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D003001:Cloning, Molecular; D015786:Cytochromes b5 / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D005615:Freezing; D005813:Genes, Synthetic; D010316:Particle Size; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D013010:Sonication
D011993:Recombinant Fusion Proteins; D015786:Cytochromes b5