title: Monkey Clara cell 10 kDa protein (CC10): a characterization of the amino acid sequence with an evolutional comparison with humans, rabbits, rats, and mice.
abstract: Monkey Clara cell 10 kDa protein (CC10) was purified from monkey lung lavage. This protein showed an apparent molecular weight of about 10 kDa and 5 kDa under non-reducing and reducing conditions, respectively, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. From the amino acid sequence data, monkey CC10 protein consisted of two identical 70-amino-acid polypeptide chains joined by two cystine residues, and possessed sequence identities of 78.6%, 52.9%, 52.9%, and 44.3% with human CC10, rat CC10 (PCB binding protein), rabbit uteroglobin, and mouse CC10, respectively. When monkey CC10 was compared with rabbit uteroglobin (progesterone binding protein), two polar residues of Tyr-21 and Thr-60, important for progesterone binding specificity, were substituted for Phe-21 and Met-60, and thus monkey CC10 may not have a binding capacity with progesterone. Monkey CC10 also possessed a surface homology with lipocortin I (anti-inflammatory peptide), thus suggesting that monkey CC10 plays a role in the anti-inflammatory process at the air-liquid interface over the bronchio-bronchiolar epithelium.
authors.affiliation: First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Sueishi
mesh_terms: D000596:Amino Acids / Q000235:genetics; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008168:Lung / Q000737:chemistry* / Q000166:cytology; D008251:Macaca; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014598:Uteroglobin / Q000235:genetics
chemical_list: D000596:Amino Acids; D011506:Proteins; C556389:SCGB1A1 protein, human; C556390:Scgb1a1 protein, mouse; C556391:Scgb1a1 protein, rat; D014598:Uteroglobin