title: Independent interaction of the acyltransferase HlyC with two maturation domains of the Escherichia coli toxin HlyA.
abstract: The apparently unique fatty acylation mechanism that underlies activation (maturation) of Escherichia coli haemolysin and related toxins is further clarified by investigation of the interaction of protoxin with the specific acyltransferase HlyC. Using deleted protoxin variants and protoxin peptides as substrates in an in vitro maturation reaction dependent upon HlyC and acyl-acyl carrier protein, two independent HlyC recognition domains were identified on the 1024-residue protoxin, proA, and they were shown to span the two target lysine residues K564 (KI) and K690 (KII) that are fatty acylated. Each domain required 15-30 amino acids for basal recognition and 50-80 amino acids for wild-type acylation. The two domains (FAI and FAII) competed with each other in cis and in trans for HlyC. The affinity of FAI for HlyC is approximately four times greater than that of FAII resulting in an overall 80% acylation at KI and 20% acylation at KII in both whole toxin and peptide derivatives. No other proA sequences were required for toxin maturation, and excess Ca2+ prevented acylation of both lysines. The lack of primary sequence identity between FAI and FAII domains in proA and among corresponding sites on related protoxins currently precludes an explanation of the basis of HlyC recognition by proA.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Stanley
authors.forename: V
authors.initials: V
authors.lastname: Koronakis
authors.forename: K
authors.initials: K
authors.lastname: Hardie
authors.forename: C
authors.initials: C
authors.lastname: Hughes
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D000217:Acyltransferases; C092237:HlyC protein, E coli; D002118:Calcium