The E5 gene of HPV-16 enhances keratinocyte immortalization by full-length DNA.
Human papillomavirus type 16 (HPV-16) is the most common papillomavirus genome found in human cervical cancers and its DNA is capable of immortalizing primary keratinocytes in vitro. When expressed by their native promoter, two separate HPV-16 oncogenes, E6 and E7, cooperate to immortalize primary human keratinocytes. Early HPV cervical lesions express abundant amounts of E5-specific RNA, and using a quantitative keratinocyte immortalization assay, we demonstrate here that E5 can act in cis to increase (4-10 fold) the efficiency of cellular immortalization by E6/E7. These results suggest that the expression of the HPV-16 E5 gene may play a role in the pathogenesis of early HPV infections by potentiating the effects of E6/E7.
Stöppler
M C
MC
Department of Pathology, Georgetown University School of Medicine, Washington, DC 20007, USA.
Straight
S W
SW
Tsao
G
G
Schlegel
R
R
McCance
D J
DJ
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics*; D002478:Cells, Cultured; D004279:DNA, Viral; D015967:Gene Expression Regulation, Viral; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000502:physiology; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D000086082:Viroporin Proteins
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C068651:E6 protein, Human papillomavirus type 16; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins; C059731:oncogene protein E7, Human papillomavirus type 16