title: Assessment of hepatic graft injury by graft effluent in rodents: N-acetyl-beta-glucosaminidase and type III procollagen peptide.
abstract: We studied the significance of N-acetyl-beta-glucosaminidase (beta-NAG) and type III procollagen peptide (P-III-P) in the effluent of rodent hepatic grafts. After total hepatectomy, the livers were preserved in chilled, lactated Ringer's solution and then divided into five groups (n = 10 each): group 1, 4 h preservation only; group 2, 4 h preservation and rewarming; group 3, 6 h preservation only; group 4, 6 h preservation and rewarming; and group 5, minimal preservation only. The beta-NAG of groups 2 and 4 was significantly higher than that of groups 1 and 3 (0.98 +/- 0.5 U/l vs 0.21 +/- 0.12 U/l; P < or = 0.01 and 1.76 +/- 0.67 U/l vs 0.38 +/- 0.25 U/l, respectively; P < or = 0.01), while that of group 4 was significantly higher than that of group 2 (1.76 +/- 0.67 U/l vs 0.98 +/- 0.50 U/l; P < or = 0.05). The P-III-P of group 4 was significantly higher than that of group 2 (0.133 +/- 0.008 U/ml vs 0.110 +/- 0.015 U/ml; P < 0.01). We conclude that beta-NAG is a novel parameter of parenchymal and nonparenchymal cells, while P-III-P reflects the integrity of the hepatic sinusoidal extracellular matrix.
authors.affiliation: Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Wakiyama
authors.forename: K
authors.initials: K
authors.lastname: Yanaga
authors.forename: Y
authors.initials: Y
authors.lastname: Soejima
authors.forename: T
authors.initials: T
authors.lastname: Nishizaki
authors.forename: T
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis*; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D006498:Hepatectomy; D007036:Hypothermia, Induced; D007552:Isotonic Solutions; D008099:Liver / Q000293:injuries* / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010446:Peptide Fragments / Q000032:analysis*; D011347:Procollagen / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000378:metabolism*; D000077325:Ringer's Lactate
chemical_list: D007552:Isotonic Solutions; D010446:Peptide Fragments; D011347:Procollagen; D000077325:Ringer's Lactate; C041876:procollagen Type III-N-terminal peptide; D000118:Acetylglucosaminidase