Influence of the terminal complement-complex on reperfusion injury, no-reflow and arrhythmias: a comparison between C6-competent and C6-deficient rabbits.
OBJECTIVE
The complement system has been suggested to play a role in reperfusion injury which may result from an enhanced destruction of myocardial tissue or from an impairment of reflow. We investigated the influence of the C5b-9 complement complex on infarct size, reflow and arrhythmogenesis.


METHODS
Twenty-eight C6-competent rabbits and 18 rabbits with congenital C6 deficiency were subjected to either 30 min or 2 h of coronary artery occlusion followed by reperfusion. C6 deficiency was confirmed by the complement titration test and immunohistology. The triphenyl tetrazolium chloride method was used to delineate infarct size. Reflow into infarcted areas was evaluated histologically after an in vivo injection of propidium iodide which served as an early fluorescence microscopic marker of damaged myocardium subjected to reflow. Continuous ECG monitoring allowed the recording of arrhythmias.


RESULTS
After 30 min of coronary artery occlusion infarct size was significantly smaller in C6-deficient rabbits (5.0 +/- 2% of the risk region) as compared to C6-competent rabbits (28.4 +/- 8.5%, P = 0.0371). The extent of reflow into damaged myocardium was nearly the same in both animal groups at this time (38 +/- 9 vs. 39 +/- 7% of the risk region). After 2 h of coronary artery occlusion, infarct size was not different between both animal groups, but the extent of reflow into damaged myocardium was significantly smaller in C6-competent rabbits than in C6-deficient rabbits (25 +/- 4 vs. 40 +/- 4%; P = 0.0185). Two of the 18 C6-deficient rabbits had ventricular arrhythmias (Lown II-IV), none of which was fatal. Eleven of the 28 C6-competent animals had major ventricular arrhythmias which were fatal in 6 rabbits.


CONCLUSIONS
These results suggest that the lytic C5b-9 complement complex leads to reperfusion injury in the early phase (30 min) of ischaemia, resulting in a larger infarct. After 2 h of ischaemia, complement activation enhances the no-reflow phenomenon but does not affect infarct size. Finally, the C6 status seems to influence the susceptibility to ventricular arrhythmias after coronary artery occlusion, independent of reperfusion.
Ito
W
W
Department of Cardiology, University Hospital Eppendorf, Hamburg, Germany.
Schäfer
H J
HJ
Bhakdi
S
S
Klask
R
R
Hansen
S
S
Schaarschmidt
S
S
Schofer
J
J
Hugo
F
F
Hamdoch
T
T
Mathey
D
D
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000276:immunology / Q000503:physiopathology; D003167:Complement Activation; D003183:Complement C6 / Q000172:deficiency*; D015938:Complement Membrane Attack Complex / Q000032:analysis*; D004562:Electrocardiography; D007150:Immunohistochemistry; D009203:Myocardial Infarction / Q000276:immunology* / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000276:immunology* / Q000503:physiopathology; D011817:Rabbits; D012039:Regional Blood Flow; D013997:Time Factors
D003183:Complement C6; D015938:Complement Membrane Attack Complex