Production of fructosyl lysine oxidase from Fusarium oxysporum S-1F4 on autoclave-browned medium.
In Fusarium oxysporum S-1F4, fructosyl lysine oxidase (FLOD) was induced with N epsilon-fructosyl N alpha-Z-lysine (epsilon-FL), which is a model compound of a glycated protein, and the induction was inhibited by the addition of cycloheximide in the growing cells. FLOD formation was greatly enhanced in an autoclave-browned medium containing glucose and L-lysine. Some Amadori compounds formed from glucose and L-lysine during autoclaving were assumed to induce the enzyme. After optimization of the culture conditions, FLOD produced in the browned medium was comparable to that in the medium with epsilon-FL.
Sakai
Y
Y
Department of Agricultural Chemistry, Kyoto University, Japan.
Yoshida
N
N
Tani
Y
Y
Kato
N
N
D000596:Amino Acids / Q000737:chemistry; D003470:Culture Media; D005670:Fusarium / Q000201:enzymology*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013242:Sterilization
D000596:Amino Acids; D003470:Culture Media; C033186:fructosyl-lysine; D010088:Oxidoreductases; D008239:Lysine