Ichnovirus infection of an established gypsy moth cell line.
In the present study, a lepidopteran cell line (Ld-652Y, from the gypsy moth, Lymantria dispar) exposed to Hyposoter fugitivus polydnavirus (HfPV) was found to display a variety of cytopathic effects. These included a transient inhibition of cell proliferation, rounding up, aggregation and apoptosis. In addition, unusual paracrystalline structures appeared within the lumen of the rough endoplasmic reticulum; similar structures were observed in the spherulocytes of parasitized Malacosoma disstria. Following Coomassie Blue staining, two new cell-associated polypeptides were detected; one of these, an 8 kDa polypeptide, could also be observed following exposure of LD-652Y cells to media taken from infected cultures or to cell-free haemolymph from parasitized M. disstria. After a period of 2-4 weeks, the L. dispar cell cultures were observed to largely recover from the effects of exposure to virus, and resumed proliferation; "transformed' cell populations tended to form aggregates, and adhered less tightly to the substrate. Viral DNA was stably maintained in all recovered cell lines, possibly in chromosomally integrated form.
Kim
M K
MK
Department of Microbiology & Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Sisson
G
G
Stoltz
D
D
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes; D009036:Moths / Q000166:cytology; D018071:Polydnaviridae / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis; D014863:Wasps / Q000821:virology
D004279:DNA, Viral; D014764:Viral Proteins