title: The LIM homeodomain protein Lim-1 is widely expressed in neural, neural crest and mesoderm derivatives in vertebrate development.
abstract: Polyclonal antibodies to Xlim-1 homeodomain protein of Xenopus laevis were used to study the developmental expression pattern of this protein in Xenopus, rat and mouse. Western blotting of embryo extracts injected with different Xlim-1 constructs confirmed the specificity of the antibody. Beginning at the gastrula stage, Xlim-1 protein was detected in three cell lineages: (i) notochord, (ii) pronephros and (iii) certain regions of the central nervous system, in agreement with earlier studies of the expression of Xlim-1 RNA (Taira et al., Development 120: 1525-1536, 1994a). In addition, several new locations of Xlim-1 expression were found, including the olfactory organ, retina, otic vesicle, dorsal root ganglia and adrenal gland. Similar expression patterns were seen for the Lim-1 protein in frog and rodent tissues. These observations implicate the Xlim-1 gene in the specification of multiple cell lineages, particularly within the nervous system, and emphasize the conserved nature of the role of this gene in different vertebrate animals.
authors.affiliation: Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Karavanov
authors.forename: J P
authors.initials: JP
authors.lastname: Saint-Jeannet
authors.forename: I
authors.initials: I
authors.lastname: Karavanova
authors.forename: M
authors.initials: M
authors.lastname: Taira
authors.forename: I B
authors.initials: IB
authors.lastname: Dawid
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D017124:Conserved Sequence; D005260:Female; D018398:Homeodomain Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice / Q000196:embryology*; D009432:Neural Crest / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats / Q000196:embryology*; D012679:Sense Organs / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013045:Species Specificity; D014157:Transcription Factors / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014981:Xenopus / Q000196:embryology*
chemical_list: D018398:Homeodomain Proteins; C091135:Isl-2 protein, vertebrate; C558561:Isl2 protein, mouse; C558562:Isl2 protein, rat; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors