Human papillomavirus type 16 E6 protein up-regulates the expression of the high mobility group protein HMG-I(Y) gene in mouse 10T1/2 cells.
Using a differential hybridization technique, we have identified a mouse cellular gene, high mobility group protein HMG-I(Y), whose expression is up-regulated by the E6 protein of human papillomavirus (HPV) type 16. This gene was overexpressed in E6-expressing mouse 10T1/2 cells, but not in G418-resistant 10T1/2 cells. The expression of the HMG-I(Y) gene was up-regulated by the transient expression of E6 from a zinc-inducible human metallothionein-IIA gene promoter. Expression was found to be more efficient at a confluent cell density than at a subconfluent cell density. The up-regulation of HMG-I(Y) gene expression by E6, in particular at a confluent cell density, may be part of an altered genetic program in host cells infected with HPV-16.
Kinoshita
T
T
Department of Microbiology, Chiba University School of Medicine, Japan.
Shirasawa
H
H
Shino
Y
Y
Shimizu
K
K
Moriya
H
H
Simizu
B
B
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D015854:Up-Regulation
C068651:E6 protein, Human papillomavirus type 16; D006609:High Mobility Group Proteins; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D025741:HMGA1a Protein