Changes in enzyme histochemical profiles of identified spinal motoneurons of the European eel, Anguilla anguilla, following cordotomy.
The enzyme histochemical profiles of glucose-6-phosphate dehydrogenase (a marker of synthetic performance), succinate dehydrogenase (an indicator of oxidative metabolism), and NADH tetrazolium reductase (a marker of overall neuronal activity) were determined for identified white muscle motoneurons in six control and six cordotomized cels. Images were digitized and mean integrated absorbances obtained using appropriate hardware and software. For motoneurons caudal to the transection site there was a significant decrease in the mean absorbance value for NADH tetrazolium reductases which declines from 0.28 a.u. (arbitrary units) in control animals to 0.23 a.u. in cordotomized animals. However, no significant changes were detected in the activities of glucose-6-phosphate and succinate dehydrogenases. The cross-sectional area of the motoneuronal cell body was not affected by cordotomy. The decrease by around 20% in overall neuronal activity, as expressed by NADH tetrazolium reductase activity, might be expected from the decline in body motility that follows cordotomy. Changes in SDH and G6PDH activities would also be expected to follow this surgery, but none were seen, perhaps because they are compensated for by changes in neuronal metabolism that result from deafferentation.
De Heus
R B
RB
Department of Experimental Zoology, University of Amsterdam, The Netherlands.
Diegenbach
P C
PC
Van Raamsdonk
W
W
Roberts
B L
BL
D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002818:Cordotomy; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D009246:NADH Tetrazolium Reductase / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D009246:NADH Tetrazolium Reductase