Nitric oxide mediates the PAF-stimulated cyclic GMP production in hippocampal slices.
Platelet activating factor (PAF) treatment caused a transient rise in cyclic GMP levels in rat hippocampal slices. The stimulation of cyclic GMP synthesis induced by PAF was dose-dependent and was suppressed after treatment with PCA-4248, a PAF antagonist, a fact that could suggest the involvement of specific PAF receptors. In addition, when slices were incubated in the presence of N-nitro-L-arginine, a nitric oxide (NO) synthase inhibitor, PAF-stimulated cyclic GMP generation was abolished. Therefore, PAF activates guanylyl cyclase most probably via formation of NO. PAF also induced a time-dependent increase of NO synthase activity in hippocampal slices in correlation with the increase observed in cyclic GMP levels.
Catalán
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RE
Departamento de Biología Molecular, Centro de Biología Molecular, Severo Ochoa (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Martínez
A M
AM
Hernández
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F
Pérez
M J
MJ
Calcerrada
M C
MC
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D006152:Cyclic GMP / Q000096:biosynthesis*; D004095:Dihydropyridines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D019335:Nitroarginine; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D004095:Dihydropyridines; D004791:Enzyme Inhibitors; D010972:Platelet Activating Factor; C066024:PCA 4248; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP