title: NCAM-mediated adhesion of transfected cells to agrin.
abstract: The vertebrate neural cell adhesion molecule NCAM mediates heterophilic adhesion to heparan sulfate proteoglycans in embryonic chick brain membranes. In this study, mouse L cells transfected with chicken NCAM were used to identify two of these ligands as agrin and the target of the 6C4 monoclonal antibody. A third heparan sulfate proteoglycan, perlecan, appeared not to support NCAM-mediated adhesion. Enzymatic degradation of chondroitin sulfates decreased adhesion in agrin-containing membrane fractions but increased adhesion if the agrin had previously been removed by immunoprecipitation, suggesting that interactions between heparan sulfate and chondroitin sulfate proteoglycans have important influences on adhesion. Our experiments support the view that NCAM can interact with multiple, but not with all, heparan sulfate and chondroitin sulfate proteoglycans in chick brain membranes in both positive and negative ways to influence cell adhesion.
authors.affiliation: Department of Developmental and Cell Biology, University of California, Irvine 92717-2300, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Storms
authors.forename: A C
authors.initials: AC
authors.lastname: Kim
authors.forename: B H
authors.initials: BH
authors.lastname: Tran
authors.forename: G J
authors.initials: GJ
authors.lastname: Cole
authors.forename: B A
authors.initials: BA
authors.lastname: Murray
mesh_terms: D018171:Agrin / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002457:Cell Extracts; D002462:Cell Membrane / Q000737:chemistry; D002642:Chick Embryo; D002808:Chondroitin Lyases; D002809:Chondroitin Sulfates / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin / Q000494:pharmacology; D019763:Heparin Lyase; D006497:Heparan Sulfate / Q000378:metabolism / Q000494:pharmacology; D007739:L Cells; D008024:Ligands; D051379:Mice; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D011133:Polysaccharide-Lyases; D011509:Proteoglycans / Q000378:metabolism; D014162:Transfection
chemical_list: D018171:Agrin; D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D002808:Chondroitin Lyases; D002809:Chondroitin Sulfates; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin; D019763:Heparin Lyase; D006497:Heparan Sulfate; D008024:Ligands; D019006:Neural Cell Adhesion Molecules; D011133:Polysaccharide-Lyases; D011509:Proteoglycans; C071927:perlecan