PCR-based restriction fragment length polymorphism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan.
A molecular typing approach for Campylobacter jejuni was applied with restriction fragment length polymorphism (RFLP) analysis of a 702-bp PCR-amplified portion of the flagellin-A (flaA) gene. We analyzed a total of 179 strains, including 69 independent clinical isolates from diarrheic patients in Japan, 85 isolates in China, and 25 heat-stable (HS) serotype strains by Penner and Hennessy (1980) J. Clin. Microbiol. 12, 732-737). Six AfaI, seven MboI, and five HaeIII RFLPs were found in the 702-bp flaA segment from the 179 strains. Using a combination of these three enzymes, 25 separate RFLP groups were recognized. While 59 of 154 (38.3%) strains obtained in Japan and China were nontypeable by the HS antigenic scheme, all but two of 154 (98.7%) could be typed by RFLP typing. All 11 isolates of HS-19 strains, which are frequently isolated from Guillain-Barré syndrome (GBS) patients, showed an identical RFLP pattern (Cj-1), and Cj-1 consisted only of HS-19 strains. This suggests that the HS-19:Cj-1 strain is distinct among C. jejuni strains. This molecular typing method provides a rapid and reliable typing scheme for epidemiological studies of C. jejuni, and may also be useful for the analysis of C. jejuni subtypes from GBS patients.
Nishimura
M
M
Department of Neurology, Utano National Hospital, Kyoto, Japan.
Nukina
M
M
Yuan
J M
JM
Shen
B Q
BQ
Ma
J J
JJ
Ohta
M
M
Saida
T
T
Uchiyama
T
T
D001431:Bacteriological Techniques; D002169:Campylobacter Infections / Q000235:genetics* / Q000276:immunology*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000276:immunology*; D002681:China; D003967:Diarrhea / Q000235:genetics* / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D005408:Flagellin / Q000235:genetics*; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011129:Polyradiculoneuropathy / Q000235:genetics; D012698:Serologic Tests
D005408:Flagellin; C067138:flaA protein, bacteria