Increase of cytochrome b mRNA by bis(2-hydroxyethyl) trisulfide in J774A.1 cells.
The effect of a cytotoxic substance (bis(2-hydroxyethyl) trisulfide; BS-1), isolated from Bacillus stearothermophilus UK563, on messenger ribonucleic acid (mRNA) level in mouse macrophage-like J774A.1 cells was investigated by the method of differential display. The treatment of J774A.1 cells with BS-1 led us to detect the inducible gene. The sequence analysis revealed that the gene was identical to mouse mitochondrial cytochrome b. In fact, Northern blot analysis showed that cytochrome b mRNA in J774A.1 cells was increased by BS-1.
Kohama
Y
Y
Division of Cellular Physiology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Iida
K
K
Itoh
S
S
Tsujikawa
K
K
Mimura
T
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D001411:Geobacillus stearothermophilus / Q000737:chemistry; D008264:Macrophages; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*
D003573:Cytochrome b Group; D012333:RNA, Messenger; D013440:Sulfides; D000431:Ethanol; C077818:bis(2-hydroxyethyl)trisulfide