Detection and in vivo hormonal regulation of rat ovarian type I and type II interleukin-I receptor mRNAs: increased expression during the periovulatory period.
OBJECTIVE
To study the expression, localization, and in vivo hormonal regulation of type I and type II interleukin-1 (IL-1) receptors in the rat ovary.


METHODS
Segments of the cDNAs for rat type I and type II IL-1 receptors were cloned and used as probes in RNase protection assays and in situ hybridization. Tissues obtained from immature rats and hormonally treated rat ovaries were examined.


RESULTS
Type I IL-1 receptor (IL-1R(1)) was ubiquitously expressed in rat tissues, including granulosa cells prepared from immature ovaries, whereas type II IL-1 receptor (IL-1R(2)) expression was restricted to macrophages, thymus, and lung. Hypophysectomy and subsequent treatment with FSH and/or diethylstilbestrol did not alter significantly the abundance of IL-1R(1) transcripts in the whole ovary. However, the relative amount of ovarian IL-1R(1) transcripts increased 7.3-fold 6 hours after the administration of hCG to pregnant mare serum gonadotropin-primed immature rats. During this time, IL-1R(1) mRNA was localized primarily in the granulosa cells. The increased expression of IL-1R(1) persisted 24 hours after hCG administration but declined to baseline by 48 hours. Ovarian expression of IL-1R(2) mRNA was observed only before ovulation in amounts that were approximately 70-fold lower than IL-1R(1).


CONCLUSIONS
The increased intraovarian expression of IL-1R(1) in granulosa cells during the periovulatory period implies that this cell type has a heightened receptivity to IL-1 and provides further indirect evidence that this cytokine is involved in the ovulatory process.
Scherzer
W J
WJ
Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore 21201-1559, USA.
Ruutiainen-Altman
K S
KS
Putowski
L T
LT
Kol
S
S
Adashi
E Y
EY
Rohan
R M
RM
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000276:immunology; D007016:Hypophysectomy; D017403:In Situ Hybridization; D008168:Lung / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D010053:Ovary / Q000276:immunology*; D010060:Ovulation / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II; D011994:Recombinant Proteins / Q000096:biosynthesis; D012016:Reference Values; D013950:Thymus Gland / Q000276:immunology; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II; D011994:Recombinant Proteins; D004054:Diethylstilbestrol; D005640:Follicle Stimulating Hormone