Autocrine response of Schizosaccharomyces pombe haploid cells to mating pheromones.
The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h- and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h- cells lacking the P-factor receptor (Mam2), they acquired mating competence in response to M-factor which they secreted. The autocrine response to P-factor in h- cells was so weak that mating competence was not acquired, although expression of the pheromone-responsive gene matl-Pm was detected. These observations support the notion that the intensity of cellular response to mating phermones is different between h- and h+ cells, although downstream pathways of the pheromone receptors are shared by the two mating types.
Kitamura
K
K
Department of Biology, Faculty of Science, Osaka City University, Japan.
Nakamura
T
T
Miki
F
F
Shimoda
C
C
D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D015870:Gene Expression; D005800:Genes, Fungal; D036341:Intercellular Signaling Peptides and Proteins; D008540:Meiosis / Q000235:genetics / Q000502:physiology; D010455:Peptides / Q000235:genetics / Q000502:physiology*; D010675:Pheromones / Q000235:genetics / Q000502:physiology*; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C089516:Mating Factor, S pombe; C085846:P-factor, S pombe; D010455:Peptides; D010675:Pheromones; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C490320:mfm1 protein, S pombe