BTF3 is evolutionarily conserved in fission yeast.
BTF3 is a protein initially identified in HeLa cells that may be involved in the initiation of transcription. Although its specific role in transcription is unclear, BTF3 can form a stable complex with RNA polymerase II. Recently, BTF3 has also been shown to bind to nascent polypeptide chains. We have cloned a homolog of BTF3 from the fission yeast, Schizosaccharomyces pombe. This homolog, spBTF3, encodes a putative 151 amino acid protein that shares 72% similarity with human BTF3, 73% similarity with the Caenorhabditis elegans homolog and between 52 and 53% similarity with the Saccharomyces cerevisiae homologs, EGD1 and BTT1.
Potashkin
J
J
Department of Pharmacology and Molecular Biology, Finch University of Health Sciences/The Chicago Medical School, IL 60064, USA. potashkj@mis.finchcms.edu
Wentz-Hunter
K
K
Callaci
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D005656:Fungal Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C063552:transcription factor BTF3