title: Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser).
abstract: The effect of light on calcium transport in mammalian sperm mitochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.
authors.affiliation: Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Breitbart
authors.forename: T
authors.initials: T
authors.lastname: Levinshal
authors.forename: N
authors.initials: N
authors.lastname: Cohen
authors.forename: H
authors.initials: H
authors.lastname: Friedmann
authors.forename: R
authors.initials: R
authors.lastname: Lubart
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D002463:Cell Membrane Permeability; D004072:Digitonin / Q000494:pharmacology; D066298:In Vitro Techniques; D007834:Lasers; D008027:Light; D008297:Male; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D004072:Digitonin; D002118:Calcium