Effects of FC43 emulsion against hyperacute rejection in rodent discordant xenotransplantation.
BACKGROUND
FC43 emulsion is one of several perfluorochemical-based artificial blood compounds developed as a red blood cell substitute. However, these compounds are not promising as red blood cell substitutes because of their immunosuppressive effects. Therefore we used FC43 emulsion as an immunosuppressive agent and not as a red blood cell substitute.


METHODS
We assessed the effect of FC43 emulsion treatment on the survival of the grafted hearts in guinea pig-to-rat discordant transplantations.


RESULTS
In rats treated with FC43 emulsion (5 microliters/gm body weight), rhythmic beating of the heart was maintained for 433.3 +/- 40.0 minutes, whereas the untreated heartbeat lasted for only 15.3 +/- 5.1 minutes. In the xenografted hearts treated with FC43 emulsion, no pathologic changes such as multiple coronary thromboses caused by hyperacute rejection had occurred by 300 minutes after reperfusion.


CONCLUSIONS
FC43 emulsion can prolong the survival time of guinea pig heart xenografts by inhibiting thrombus formation in the xenografted heart.
Wada
S
S
First Department of Surgery, Hiroshima University, School of Medicine, Japan.
Kajihara
H
H
Murakami
H
H
Sueda
T
T
Matsuura
Y
Y
D000818:Animals; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D006084:Graft Rejection / Q000473:pathology; D006085:Graft Survival / Q000187:drug effects*; D006168:Guinea Pigs; D016027:Heart Transplantation; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous
D004655:Emulsions; D005466:Fluorocarbons; D007166:Immunosuppressive Agents; C008807:perfluorotributylamine