IL-2 receptor gamma chain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood.
The IL-2 receptor (IL-2R) gamma chain is shared among receptors for IL-4, IL-7, IL-9 and IL-15 as well as IL-2. In order to clarify the functional role of these cytokines interacting with the common gamma chain in human early hematopoiesis, we studied expression of the IL-2R gamma chain on purified CD34 positive cells from bone marrow and cord blood. Broad populations of bone marrow mononuclear cells were all found to express the IL-2R gamma chain. CD34 positive cells were purified by CD34 monoclonal antibodies and immunomagnetic beads as representative hematopoietic progenitor cells. It was established that only 38 +/- 10% of CD34 positive bone marrow cells (n = 5) and 35 +/- 12% of CD34 positive cord blood cells (n = 11) expressed the IL-2R gamma chain. CD34(+) IL-2R gamma chain(+) and CD34(+) IL-2R gamma chain(-) cells fractionated by cell sorting were subjected to clonogenic assays that showed granulocyte-macrophage colony-forming cells (CFU-GM) were present evenly in both fractions, whereas erythroid burst-forming cells (BFU-E) were enriched in the CD34(+) IL-2R gamma chain(-) fraction approximately two- to six-fold as compared with CD34(+) IL-2R gamma chain(+) fraction. Such clonogenic features did not differ between the bone marrow and cord blood cases. These results indicate that CD34(+) IL-2R gamma chain(-) cells contain immature cells already committed to the erythroid lineage.
Itano
M
M
Department of Pediatric Oncology, Tohoku University, Sendai, Japan.
Tsuchiya
S
S
Morita
S
S
Fujie
H
H
Ishii
N
N
Yanagisawa
T
T
Ohashi
Y
Y
Minegishi
M
M
Sugamura
K
K
Konno
T
T
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34 / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells; D019070:Cell Lineage; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D015375:Receptors, Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016650:Fluorescein-5-isothiocyanate