A simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from Trichosanthes kirilowii root tubers.
A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosanthes kirilowii has been developed using cation-exchange perfusion chromatography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information. Yields of 0.16% of electrophoretically pure trichosanthin from dried root tuber have been achieved with a single 10-min chromatographic step giving the ability to purify gram quantities of trichosanthin in 1 day.
Bhatia
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Department of Chemical Engineering and Materials Science, University of California at Davis 95616, USA.
McDonald
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Jackman
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Dandekar
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AM
D000019:Abortifacient Agents / Q000302:isolation & purification; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D010940:Plant Proteins / Q000302:isolation & purification; D010944:Plants / Q000378:metabolism; D015978:Trichosanthin / Q000302:isolation & purification*
D000019:Abortifacient Agents; D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D015978:Trichosanthin