Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes.
Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method.
China
B
B
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Belgium.
Pirson
V
V
Mainil
J
J
D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002352:Carrier Proteins; D002417:Cattle; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014774:Virulence
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C073819:eaeA protein, E coli