Molecular study of nosocomial nocardiosis outbreak involving heart transplant recipients.
Randomly amplified polymorphic DNA analysis and rRNA gene restriction patterns (ribotyping) were compared as methods of investigating a nosocomial outbreak of nocardiosis involving three heart transplant recipients. No clear distinctions between three clinically related isolates and four unrelated strains were obtained by ribotyping. On the contrary, randomly amplified polymorphic DNA analysis with two selected primers, primers 2650 and DKU49, showed one pattern for the three related isolates and four patterns for the unrelated strains.
Exmelin
L
L
Laboratoire de Microbiologie, Centre-Hospitalo-Universitaire Côte de Nacre, Caen, France.
Malbruny
B
B
Vergnaud
M
M
Prosvost
F
F
Boiron
P
P
Morel
C
C
D000328:Adult; D001483:Base Sequence; D003428:Cross Infection / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes; D004196:Disease Outbreaks; D005069:Evaluation Studies as Topic; D005602:France / Q000453:epidemiology; D016027:Heart Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009615:Nocardia / Q000235:genetics / Q000302:isolation & purification; D009617:Nocardia Infections / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D012335:RNA, Ribosomal / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes