Evidence of presence of poliovirus genomic sequences in cerebrospinal fluid from patients with postpolio syndrome.
The postpolio syndrome (PPS) is characterized by new neuromuscular symptoms occurring 30 to 40 years after the acute episode of poliomyelitis paralysis. The presence of the poliovirus RNA genome in the cerebrospinal fluid from 10 patients with PPS and from 23 control patients was sought by using reverse transcription and a PCR specific for polioviruses and/or other enteroviruses. Poliovirus-specific genomic sequences in the 5' untranslated region and in the capsid region (VP1) were detected by reverse transcription PCR in 5 of 10 patients with PPS but in none of the control patients. Sequencing confirmed the presence of mutated poliovirus sequences. This finding suggests persistent viral infection in the central nervous system related to the presence of poliovirus genomes.
Leparc-Goffart
I
I
Laboratoire de Virologie, Centre National de Référence des Entérovirus, Lyon, France. leparc@cismsun.univ-lyon1.fr
Julien
J
J
Fuchs
F
F
Janatova
I
I
Aymard
M
M
Kopecka
H
H
D000328:Adult; D000368:Aged; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017955:Poliovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D016262:Postpoliomyelitis Syndrome / Q000134:cerebrospinal fluid* / Q000473:pathology; D012367:RNA, Viral / Q000134:cerebrospinal fluid*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014776:Virus Cultivation
D012367:RNA, Viral