Reduced germline mobility of a mariner vector containing exogenous DNA: effect of size or site?
Germline mobilization of the transposable element mariner is severely inhibited by the insertion of a 4.5- to 11.9-kb fragment of exogenous DNA into a unique SacI site approximately in the middle of the 1286-bp element. In the presence of transposase driven by the germline-specific hsp26-sgs3 promoter, mobilization of the MlwB construct (containing a 11.9-kb insertion) is detected at low frequency. Analysis of a mobilized MlwB element indicated that mobilization is mediated by the mariner transposase. However, transposed MlwB elements are also defective in germline mobilization. Rare, transposase-induced germline excision events were also recovered for such vectors. The estimated rate of excision is < 0.1% per chromosome per generation. Excision appears to be accompanied by gap repair if a suitable template is available. The data imply that the reduced mobility of mariner vectors with exogenous DNA in the SacI site results from disruption of sequences necessary for efficient mobilization. The relative stability may be a valuable property in the uses of mariner-like elements in genetic engineering of insects of economic importance.
Lohe
A R
AR
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Hartl
D L
DL
D000818:Animals; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D008297:Male; D008969:Molecular Sequence Data; D014170:Transformation, Genetic; D019895:Transposases
D004251:DNA Transposable Elements; D004254:DNA Nucleotidyltransferases; D019895:Transposases