Capillary electrophoresis with laser-induced fluorescence detection: a sensitive method for monitoring extracellular concentrations of amino acids in the periaqueductal grey matter.
The use of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the analysis of microdialysate samples from the periaqueductal grey matter (PAG) of freely moving rats is described. By employing 3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) as a derivatization agent, we simultaneously monitored the concentrations of 8 amino acids (arginine, glutamine, valine, gamma-amino-n-butyric acid (GABA), alanine, glycine, glutamate, and aspartate), with nanomolar and subnanomolar detection limits. Two of the amino acids (GABA and glutamate) were analysed in parallel by conventional high-performance liquid chromatography (HPLC) in order to directly compare the two analytical methods. Other CE methods for analysis of microdialysate have been previously described, and this improved method offers greater sensitivity, ease of use, and the possibility to monitor several amino acids simultaneously. By using this technique together with an optimised form of microdialysis technique, the tiny sample consumption and the improved detection limits permit the detection of fast and transient transmitter changes.
Bergquist
J
J
Department of Psychiatry and Neurochemistry, Göteborg University, Mölndal Hospital, Sweden. jonas.bergquist@bll.se
Vona
M J
MJ
Stiller
C O
CO
O'Connor
W T
WT
Falkenberg
T
T
Ekman
R
R
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001565:Benzoates; D002118:Calcium / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000378:metabolism; D007834:Lasers; D008297:Male; D017551:Microdialysis; D010487:Periaqueductal Gray / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011804:Quinolines; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D001565:Benzoates; D005456:Fluorescent Dyes; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002118:Calcium