title: Creation of deletion, insertion and substitution mutations using a single pair of primers and PCR.
authors.affiliation: Department of Biochemistry, McMaster University Hamilton, ON, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hughes
authors.forename: D W
authors.initials: DW
authors.lastname: Andrews
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000737:chemistry / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D017931:DNA Primers; D011498:Protein Precursors; C017671:preprolactin; D011388:Prolactin