Hypericin site specific interactions within polynucleotides used as DNA model compounds.
Interactions of the antiretroviral hypericin molecule with polynucleotides, i.e. poly(dG-dC), poly(dA-dT), poly(rG) and poly(rC), have been studied in aqueous solutions by resonance Raman spectroscopy, using an UV excitation wavelength which induces a specific resonance enhancement of spectral band intensities corresponding to proper nucleic base modes of vibration. It is shown that: i) hypericin selectively interacts with the N7 sites of purines, ii) the strength of interaction depends on the polymer structure, and: iii) interaction with guanine is stronger than with adenine molecules.
Miskovsky
P
P
Biophysics Division, Safarik University, Kosice, Slovakia. misko@kosice.upjs.sk
Chinsky
L
L
Wheeler
G V
GV
Turpin
P Y
PY
D000873:Anthracenes; D000998:Antiviral Agents / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D006147:Guanine; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D011068:Poly G / Q000378:metabolism; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism*
D000873:Anthracenes; D000998:Antiviral Agents; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; D011068:Poly G; D011067:Poly dA-dT; C019530:poly(dC-dG); D011066:Poly C; D010569:Perylene; D006147:Guanine; C004965:hypericin