title: Dietary calcium salts as protective agents and laminin P1 as a biochemical marker in chemically induced colon carcinogenesis in rats.
abstract: To evaluate the protective effect of different calcium forms against colon carcinogenesis, Wistar rats fed a high-fat diet (24%) were supplemented with different chemical forms of dietary calcium and were intrarectally instilled with N-methyl-N-nitrosourea (NMU). Supplemental calcium was administered at 1.5% mineral (w/w of total diet) complexed with either carbonate, gluconate, or lactate in Groups 2, 3, and 4, respectively. The tumor incidence of colon cancer was compared with a control group (Group 1), fed the same diet without supplemental calcium. Colon carcinoma incidence was 31, 33, 13, and 7% in Groups 1, 2, 3, and 4, respectively. Calcium had a significant protective effect against carcinogenesis, and the maximum protective effect was observed with gluconate and lactate forms. Laminin P1 blood level was measured as a tumor marker. Laminin P1 results were compared with the reference group (Group T), fed a standard diet and not NMU instilled. The serum laminin P1 level was significantly higher (p = 0.0001) in NMU-instilled Groups 1, 2, 3, and 4 (0.24 +/- 0.03, 0.93 +/- 1.43, 0.84 +/- 1.33, and 0.41 +/- 0.34 mU/ml respectively) than in the Reference Group T (0.10 +/- 0.05 mU/ml).
authors.affiliation: Human Nutrition Center, Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Belbraouet
authors.forename: F
authors.initials: F
authors.lastname: Felden
authors.forename: X
authors.initials: X
authors.lastname: Pelletier
authors.forename: I
authors.initials: I
authors.lastname: Gastin
authors.forename: D
authors.initials: D
authors.lastname: Lambert
authors.forename: J
authors.initials: J
authors.lastname: Floquet
authors.forename: J L
authors.initials: JL
authors.lastname: Guéant
authors.forename: G
authors.initials: G
authors.lastname: Debry
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D001835:Body Weight; D002136:Calcium, Dietary / Q000008:administration & dosage*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D007797:Laminin / Q000097:blood*; D008297:Male; D010446:Peptide Fragments / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D014408:Biomarkers, Tumor; D002136:Calcium, Dietary; D007797:Laminin; D010446:Peptide Fragments; C055716:laminin P1