title: Development of a PCR-based diagnostic assay for the determination of KEL genotype in donor blood samples.
abstract: The polymorphism which determines expression of Kell and Cellano antigens on the redcell surface has been reported to be a single C-->T nucleotide substitution at residue 701 where T codes for the presence of Kell antigen. This was confirmed by the direct automated sequencing of PCR products amplified from individuals of known Kell phenotype. The substitution creates a Bsm I restriction enzyme site and this has formed the basis for the development of a PCR-based diagnostic assay for the determination of Kell phenotype in samples of donor blood. The assay is based on RFLP analysis of coamplified PCR products, one of which spans the K/k polymorphic site, and one control fragment which contains a Bsm I site. Digestion of the PCR products with Bsm I restriction enzyme and subsequent gel analysis of the digest allowed unequivocal determination of the K/k status of all of the samples tested.
authors.affiliation: Glasgow and West of Scotland Blood Transfusion Service, Law Hospital, Lanarkshire, U.K.
authors.forename: M T
authors.initials: MT
authors.lastname: Murphy
authors.forename: R H
authors.initials: RH
authors.lastname: Fraser
authors.forename: J P
authors.initials: JP
authors.lastname: Goddard
mesh_terms: D001782:Blood Donors; D004912:Erythrocytes / Q000276:immunology*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D007626:Kell Blood-Group System / Q000235:genetics* / Q000276:immunology; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D007519:Isoantigens; D007626:Kell Blood-Group System