EFFECTS OF HISTAMINE AND RELATED COMPOUNDS ON THE BOVINE IRIS DILATOR.
Effects of histamine and related compounds on the bovine iris dilator were investigated. Histamine caused a concentration-related contraction of the bovine iris dilator and IC50 was 1.57 x 10(-7) M. The potency of histamine on the bovine iris dilator was almost the same as that observed in guinea pig ileum. Histamine-induced contraction of the bovine iris dilator was antagonized by the H1 antagonists pyrilamine, diphenhydramine and chlorpheniramine, whereas pretreatment with the H2 antagonists cimetidine and ranitidine was most effective. In addition, histamine and the H1 agonist 2-methylhistamine caused a contraction of bovine iris dilator, but the H2 agonist 4-methylhistamine was not effective. An H3 antagonist, thioperamide, also had no contractive effects on the bovine iris dilator. The bovine iris dilator contained a considerable amount of histamine, which was not released by compound 48/80, substance P or by increasing K+ concentration in the medium. In conclusion, histamine caused a potent contraction of the bovine iris dilator via H1 receptor, and this muscle showed a high sensitivity to histamine similar to guinea pig ileum.
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D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002744:Chlorpheniramine / Q000494:pharmacology; D002927:Cimetidine / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects* / Q000378:metabolism; D007498:Iris / Q000187:drug effects* / Q000378:metabolism; D007928:Lethal Dose 50; D008297:Male; D008761:Methylhistamines / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010880:Piperidines / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011738:Pyrilamine / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects* / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D008761:Methylhistamines; D010880:Piperidines; D011969:Receptors, Histamine H1; D013373:Substance P; D002744:Chlorpheniramine; D003189:p-Methoxy-N-methylphenethylamine; C025566:4-methylhistamine; D002927:Cimetidine; D006632:Histamine; D011899:Ranitidine; D004155:Diphenhydramine; C026259:2-methylhistamine; D011738:Pyrilamine; C052075:thioperamide; D011188:Potassium