[Allo-cytotoxic T lymphocyte responses in corneal allografted mice treated with monoclonal antibodies to adhesion molecules].
Corneal transplantation was performed by grafting C3H/He donor corneas into BALB/c corneal beds. The allografted mice were injected either with 0.5 mg/day of anti-very late antigen (VLA)-4 antibody, anti-leukocyte function-associated antigen (LFA)-1 antibody, or 0.25 mg/day each of both antibodies on days -2, 0, 1, 3, 5, and 7. After 3 weeks, cytotoxic T lymphocyte (CTL) responses to donor alloantigens were assessed. Splenocytes in the allografted mice without treatment demonstrated greater CTL responses that those in naive mice. CTL responses were depressed in mice treated with either anti-LFA-1 alone or a combination of anti-LFA-1 and anti-VLA-4 antibodies as compared with splenocytes from allografted mice without treatment. These data suggest that CTLs play significant roles in eliciting immune response to corneal allografts.
Hori
J
J
Department of Ophthalmology, Fraternity Memorial Hospital, Tokyo, Japan.
Isobe
M
M
Mizuochi
T
T
Yamagami
S
S
Tsuru
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology* / Q000627:therapeutic use; D003315:Cornea / Q000276:immunology*; D016039:Corneal Transplantation / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D003864:Depression, Chemical; D006084:Graft Rejection / Q000628:therapy; D039041:Integrin alpha4beta1; D016023:Integrins / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016201:Receptors, Lymphocyte Homing / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D039041:Integrin alpha4beta1; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing