title: Regulation of cADP-ribose-induced Ca2+ release by Mg2+ and inorganic phosphate.
abstract: cADP-ribose (cADPr) has recently been shown to release Ca2+ from an intracellular store of permeabilized T lymphocyte cell lines (Guse, A. H., da Silva, C. P., Emmrich, F., Ashamu, G. A., Potter, B. V. L., and Mayr, G. W. (1995) J. Immunol. 155, 3353-3359). Using permeabilized Jurkat and HPB. ALL T lymphocytes, the effects of varying concentrations of inorganic phosphate and Mg2+ on cADPr-induced Ca2+ release were investigated. cADPr-induced Ca2+ release was dependent on the concentration of inorganic phosphate, showing very low Ca2+ release activity between 0.5 and 2 mM inorganic phosphate. At 4 to 5 mM inorganic phosphate, the cADPr-induced Ca2+ release was much more pronounced, reaching maximal values at 10 mM inorganic phosphate. The underlying mechanism for this stimulatory effect was an increased loading of the cADPr-sensitive Ca2+ store, which was demonstrated by enhanced resequestration of Ca2+ selectively into the cADPr-sensitive Ca2+ store. The free Mg2+ concentration also influenced cADPr-induced Ca2+ release in permeabilized cells: at 0 and 8.58 mM the release was nearly completely abolished, whereas at 1.06 mM maximal Ca2+ release by cADPr was observed. High performance liquid chromatographic analysis of exogenously added cADPr revealed that the catabolism of cADPr at varying Mg2+ and Pi concentrations had only minor relevance for the modulatory effects observed. To correlate the effects of inorganic phosphate and Mg2+ on cADPr-induced Ca2+ release observed in the permeabilized cell preparations, measurements of these ions in intact Jurkat T lymphocytes were carried out. Intact Jurkat T cells stimulated via the T cell receptor middle dotCD3 complex did not respond with significant elevation of the free intracellular Mg2+ concentration. In contrast, stimulation via the T cell receptor middle dotCD3 complex resulted in an increase in the intracellular inorganic phosphate concentration. These data indicate a role for the intracellular inorganic phosphate concentration in the regulation of cADPr-mediated Ca2+ release in T lymphocytes.
authors.affiliation: University of Hamburg, Institute of Physiological Chemistry, Department of Enzyme Chemistry, Grindelallee 117, D-20146 Hamburg, Germany.
authors.forename: A H
authors.initials: AH
authors.lastname: Guse
authors.forename: C P
authors.initials: CP
authors.lastname: Silva
authors.forename: K
authors.initials: K
authors.lastname: Weber
authors.forename: G A
authors.initials: GA
authors.lastname: Ashamu
authors.forename: B V
authors.initials: BV
authors.lastname: Potter
authors.forename: G W
authors.initials: GW
authors.lastname: Mayr
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation; D002460:Cell Line; D036563:Cyclic ADP-Ribose; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D015290:Second Messenger Systems; D013601:T-Lymphocytes
chemical_list: D010710:Phosphates; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D015544:Inositol 1,4,5-Trisphosphate; D008274:Magnesium; D002118:Calcium