Human papillomavirus type 70 genome cloned from overlapping PCR products: complete nucleotide sequence and genomic organization.
The genome of human papillomavirus (HPV) type 70 (HPV 70), isolated from a cervical condyloma, was obtained by cloning overlapping PCR products. By automated DNA sequence analysis, the genome was found to consist of 7,905 bp with a G + C content of 40%. The genomic organization showed the characteristic features shared by other sequenced HPVs. Nucleotide sequence comparison with previously known HPV types demonstrated the closest homology with HPV 68 (82%), HPV 39 (82%), HPV 18 (70%), HPV 45 (70%), and HPV 59 (70%). Comparison with seven other partially sequenced HPV 70 isolates showed homologies of between 100 and 99.5%. Cloning of overlapping PCR products and automated DNA sequence analysis was found to be a feasible method of obtaining full-length sequences of HPVs.
Forslund
O
O
Department of Medical Microbiology, Lund University, Malmö University Hospital, Sweden. mblofd@mascan.mas.lu.se
Hansson
B G
BG
D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D003218:Condylomata Acuminata / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D002577:Uterine Cervical Diseases / Q000821:virology
D018387:Codon, Initiator; D018388:Codon, Terminator; D017931:DNA Primers; D004279:DNA, Viral