title: The effect of intestinal reperfusion on renal function and perfusion.
abstract: This study examines the effect of intestinal reperfusion injury (IIR) on renal blood flow and relates this temporally to changes in renal ATP levels and renal tubular function. Sprague-Dawley rats underwent 120 min of intestinal ischemia and 60 min of reperfusion (IIR). Renal blood flow was measured with radiolabeled microspheres and a Doppler flow probe. Renal dysfunction was quantitated by measuring inulin clearance and fractional excretion of sodium (FENa) in the isolated perfused organ. Renal tissue ATP levels were measured using a luciferase-luciferin assay. Sham-operated animals served as controls (SHAM). Renal blood flow was reduced by > 80% in the animals sustaining IIR when compared to baseline measurements (P < 0.05) or SHAM (P < 0.05). Temporally this reduction in renal blood flow was associated with a 25% reduction in tissue ATP levels (P < 0.05). The kidneys of animals sustaining IIR had a significantly greater FENa than did those of controls. These data support the notion that IIR is associated with a profound reduction in renal blood flow which is temporally related to reduced renal tissue ATP levels and renal tubular dysfunction.
authors.affiliation: Department of Surgery, University of Texas Southwestern Medical School, Dallas 75235, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: LaNoue
authors.forename: R H
authors.initials: RH
authors.lastname: Turnage
authors.forename: K M
authors.initials: KM
authors.lastname: Kadesky
authors.forename: K S
authors.initials: KS
authors.lastname: Guice
authors.forename: K T
authors.initials: KT
authors.lastname: Oldham
authors.forename: S I
authors.initials: SI
authors.lastname: Myers
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000503:physiopathology; D001794:Blood Pressure; D007422:Intestines / Q000098:blood supply*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008863:Microspheres; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D000255:Adenosine Triphosphate