Variability in 3' end of 16S rRNA sequence of Mycobacterium ulcerans is related to geographic origin of isolates.
Mycobacterium ulcerans causes extensive ulcers (Buruli ulcers) in the skin of humans. Analysis of the 3'-terminal region of the 16S rRNA gene sequence of 17 strains of M. ulcerans from Africa, the Americas, and Australia revealed three subgroups corresponding to the continent of origin, and some variable phenotypic characteristics. This sequence is useful for the rapid detection of M. ulcerans and discriminates M. marinum and M. shinshuense from M. ulcerans.
Portaels
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F
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
Fonteyene
P A
PA
de Beenhouwer
H
H
de Rijk
P
P
Guédénon
A
A
Hayman
J
J
Meyers
M W
MW
D000349:Africa / Q000453:epidemiology; D000569:Americas / Q000453:epidemiology; D001315:Australia / Q000453:epidemiology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012883:Skin Ulcer / Q000453:epidemiology / Q000382:microbiology; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S