title: Kinetics of inhibition of alkaline phosphatase from green crab (Scylla serrata) by N-bromosuccinimide.
abstract: The inactivation of alkaline phosphatase from green crab (Scylla serrata) by N-bromosuccinimide has been studied using the kinetic method of the substrate reaction during modification of enzyme activity previously described by Tsou [(1988), Adv. Enzymol. Related Areas Mol. Biol. 61, 381-436]. The results show that inactivation of the enzyme is a slow, reversible reaction. The microscopic rate constants for the reaction of the inactivator with free enzyme and the enzyme-substrate complex were determined. Comparison of these rate constants indicates that the presence of substrate offers marked protection of this enzyme against inactivation by N-bromosuccinimide. The above results suggest that the tryptophan residue is essential for activity and is situated at the active site of the enzyme.
authors.affiliation: Department of Biology, Xiamen University, China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: W Z
authors.initials: WZ
authors.lastname: Zheng
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: S X
authors.initials: SX
authors.lastname: Yan
authors.forename: H R
authors.initials: HR
authors.lastname: Wang
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D001665:Binding Sites; D003386:Brachyura / Q000201:enzymology*; D001974:Bromosuccinimide / Q000494:pharmacology; D007700:Kinetics; D008956:Models, Chemical; D009596:Nitrophenols / Q000378:metabolism / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
chemical_list: D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D014364:Tryptophan; D000469:Alkaline Phosphatase; D001974:Bromosuccinimide