Immunopharmacological activities of the nontoxic monophosphoryl lipid A of Porphyromonas gingivalis.
The low endotoxic lipid A derived from Porphyromonas gingivalis 381, 1-phospho beta(1-6)-linked glucosamine disaccharide with 3-hydroxy-15-methylhexadecanoyl and 3-hexadecanoyloxy-15-methylhexadecanoyl groups at the 2- and 2'-positions, respectively, induced mitogenic responses in LPS low responder C3H/HeJ as well as LPS responder C3H/HeN mouse splenocytes. The mitogenic activities of P. gingivalis lipid A in splenocytes of LPS responder mice were comparable to that of the synthetic Escherichia coli-type lipid A (compound 506). The addition of polymyxin B resulted in the inhibition of mitogenic activity. P. gingivalis lipid A also stimulated strongly nonspecific host resistance against Pseudomonas aeruginosa infection in BALB/c mice, and specific immune response in guinea pigs against infection with P. gingivalis. Furthermore, P. gingivalis lipid A demonstrated antitumour activity against MH134 hepatoma in C3H/HeN mice. These life-preserving with tumour regression properties were comparable to those of monophosphoryl lipid A derived from Salmonella minnesota Re 595. Thus, P. gingivalis lipid A appears to have beneficial properties as an immunopharmacological agent.
Ogawa
T
T
Department of Oral Microbiology, Osaka University, Japan.
Nakazawa
M
M
Masui
K
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D016866:Bacteroidaceae Infections / Q000517:prevention & control; D006168:Guinea Pigs; D007113:Immunity, Innate; D008050:Lipid A / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000517:prevention & control; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D008050:Lipid A; C048436:monophosphoryl lipid A