title: A possible tyrosine phosphorylation of phytochrome.
abstract: Red/far-red light signal transduction by the phytochrome family of photoreceptors regulates plant growth and development. We investigated the possibility that tyrosine kinases and/or phosphatases are involved in phytochrome-mediated signal transduction using crude extracts of oat seedlings that are grown in the dark. We found that a 124 kDa protein was tyrosine-phosphorylated as determined by Western blotting with a phosphotyrosine-specific monoclonal antibody. The 124 kDa protein was recognized by the anti-phosphotyrosine antibody in anti-phytochrome A immunoprecipitates. The level of anti-phosphotyrosine antibody binding to the 124 kDa protein(s) in phytochrome immunoprecipitates that had been treated with red light prior to immunoprecipitation decreased relative to dark controls. These results suggest that either phytochrome from dark-grown seedlings is tyrosine phosphorylated or that it co-immunoprecipitates with a phosphotyrosine-containing protein of the same molecular weight. The implications of these results in the regulation of (a) the putative Ser/Thr kinase activity of the photoreceptor and (b) the binding of signaling molecules, such as phospholipase C to phytochrome, are discussed.
authors.affiliation: Department of Chemistry, University of Nebraska-Lincoln 68588, USA.
authors.forename: D
authors.initials: D
authors.lastname: Sommer
authors.forename: T A
authors.initials: TA
authors.lastname: Wells
authors.forename: P S
authors.initials: PS
authors.lastname: Song
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D018554:Avena / Q000254:growth & development; D015153:Blotting, Western; D003624:Darkness; D008027:Light; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis*; D010834:Phytochrome / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014443:Tyrosine
chemical_list: D000911:Antibodies, Monoclonal; D010834:Phytochrome; D019000:Phosphotyrosine; D014443:Tyrosine