rbohA, a rice homologue of the mammalian gp91phox respiratory burst oxidase gene.
It has been hypothesized that plants contain respiratory burst oxidase which, upon activation, oxidize NADPH and generate extracellular superoxide, O2.-. These proteins are proposed to play a central role in defence against pathogens. However, plant DNA sequences that encode proteins with similarity to components of respiratory burst oxidase have not previously been reported. This paper describes the complete cDNA and genomic DNA sequence of the rice rbohA (for respiratory burst oxidase homologue) gene. The predicted RbohA product is most similar to the main catalytic subunit, gp91phox, of the respiratory burst oxidase of neutrophils. Reverse transcriptase PCR detects rbohA transcripts in both roots and shoots of healthy rice plants.
Groom
Q J
QJ
Department of Biochemistry and Genetics, University of Newcastle, UK. Q.J.Groom@newcastle.ac.uk
Torres
M A
MA
Fordham-Skelton
A P
AP
Hammond-Kosack
K E
KE
Robinson
N J
NJ
Jones
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D008322:Mammals / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018744:DNA, Plant; D008562:Membrane Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D009247:NADH, NADPH Oxidoreductases; C103489:RbohA protein, Oryza sativa; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C022709:superoxide-forming enzyme