In situ hybridization study of vascular cell adhesion molecule-1 messenger RNA expression in rheumatoid synovium.
Upregulation of vascular cell adhesion molecule-1 (VCAM-1) has been implicated in various pathological conditions. In this study, we used in situ hybridization to determine the cell types expressing VCAM-1 mRNA in rheumatoid synovium. Synovial tissues from seven rheumatoid arthritis (RA) and four osteoarthritis (OA) patients were examined for VCAM-1 mRNA expression. In situ hybridization as well as immunohistochemical analysis showed that VCAM-1 expression was mainly localized to the hyperplastic synovial lining cells and to a lesser extent endothelial cells and synovial fibroblasts in rheumatoid synovium. VCAM-1 expression in OA is less prominent. Hyperplastic synovial lining cells expressing VCAM-1 were mainly CD13+, CD14+, CD33+ and HLA-DR+. Together with morphological features, this suggests that they are type A macrophage-like synovial cells. Our findings indicate that overexpression of VCAM-1 may contribute to the formation of hyperplastic lining layers in the synovium seen in this disorder.
Higashiyama
H
H
Division of Immunological Diseases, Medical Research Institute, Tokyo, Japan.
Saito
I
I
Hayashi
Y
Y
Miyasaka
N
N
D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D010003:Osteoarthritis / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012218:Rheumatoid Nodule / Q000276:immunology* / Q000473:pathology; D013583:Synovial Membrane / Q000276:immunology* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*
D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1