title: Use of micellar media for the fluorimetric determination of ellipticine in aqueous solutions.
abstract: Ellipticine is a pyridocarbazole alkaloid with interesting antitumour activity. Use of neutral ellipticine is hampered by its very low water solubility and therefore this compound has been administered as a salt; however, nitrogen quaternization modifies the antitumour properties of ellipticine. Potential alternatives to quaternization include the use of cyclodextrins, and also the use of micellar media. The latter possibility is explored in this work as an analytical tool. The results obtained with model anionic (SDS), cationic (CTAB) and neutral (Brij-35) surfactants are described. Fluorimetric analysis shows that ellipticine solubilizes completely in the presence of all these compounds, as a result of its aromatic, planar structure. The use of micellar media considerably increases the slopes of the calibration curves with improved correlation coefficients (e.g. 0.8904 in water and 0.9982 with SDS). Micellar media also modify proton transfer processes, as a consequence of the apolar environment of the micellar phase. Deprotonation of ellipticine is hampered in SDS because of the relationship between this process and the surface charge of the micelles. Finally, fluorescence quenching in micellar media has been studied, it being found that surfactants provide protection towards this phenomenon.
authors.affiliation: Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sbai
authors.forename: S A
authors.initials: SA
authors.lastname: Lyazidi
authors.forename: D A
authors.initials: DA
authors.lastname: Lerner
authors.forename: B
authors.initials: B
authors.lastname: del Castillo
authors.forename: M A
authors.initials: MA
authors.lastname: Martin
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000737:chemistry; D004611:Ellipticines / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D005470:Fluorometry; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D004611:Ellipticines; D008823:Micelles; C034192:ellipticine