Presence of MHC-I and B-7 molecules in rat and human glioma cells expressing antisense IGF-I mRNA.
Tumor cells frequently express the insulin-like growth factor I (IGF-I). Recently we demonstrated that rat glioma cells when transfected with a vector encoding antisense IGF-I cDNA lost tumorigenicity and induced a tumor specific immune response involving CD8+ lymphocytes. Here we show that the cultured transfected cell lines, rat C-6 glioma, human primary glioma and mouse teratocarcinoma, expressed an increased level of MHC-I and of co-signaling B-7 molecules. This increased expression of MHC-I and B-7, demonstrated by 51Cr release complement dependent cytoxicity assay and by immunostaining flow cytometry analysis, could contribute to the final immune recognition of glioma immunogenicity.
Trojan
J
J
C.H.B. Hôpital Paul Brousse 12, Université Paris-Sud, Villejuif, France.
Duc
H T
HT
Upegui-Gonzalez
L C
LC
Hor
F
F
Guo
Y
Y
Anthony
D
D
Ilan
J
J
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018122:B7-1 Antigen / Q000032:analysis*; D051940:B7-2 Antigen; D005434:Flow Cytometry; D015870:Gene Expression / Q000502:physiology; D005910:Glioma; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D009447:Neuroblastoma; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D018243:Teratocarcinoma; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000502:physiology
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; C497965:Cd86 protein, mouse; C497966:Cd86 protein, rat; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II