title: Presence of antibodies to p21X and/or p27rex proteins in sera from human T-cell leukemia virus type I-infected individuals.
abstract: The human T-cell leukemia virus type I (HTLV-I) pX gene encodes three nonstructural proteins, p40tax, p27rex and p21X. So far, natural antibodies to p27rex and/or p21X have not been found in sera from HTLV-I-infected individuals, although antibodies to p40tax have been found. Recently, the viral transcripts specific for these proteins were detected in fresh peripheral blood mononuclear cells from HTLV-I-infected individuals by the polymerase chain reaction coupled to reverse transcription, showing the in vivo expression of these proteins. We detected antibodies to p21X and p27rex by an enzyme-linked immunosorbent assay (ELISA) system using a recombinantly produced p21X protein as a common antigen, because p21X is identical to the C-terminal portion of p27rex. The sensitivity of the ELISA was determined to be approximately 100 times greater than that of Western blotting. From the analyzed sera of 31 ATL patients, 30 asymptomatic carriers, 18 HAM patients and 100 healthy donors, three specimens from one ATL patient and two carriers were found to be positive for anti-p21X/p27rex antibodies. The specificity of the ELISA reaction was confirmed by the competitive ELISA test with the highly purified recombinant p21X protein. As of result, we first determined the presence of anti-p21X/p27rex antibodies in a small percentage (3.8%) of the sera from HTLV-I-infected individuals. Even sera from the ATL patients, whose fresh PBMCs contained the transcripts for these proteins, were not found to contain these antibodies, suggesting that the immune response to these proteins is low in HTLV-I-infected humans.
authors.affiliation: Shionogi Institute for Medical Science, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Saiga
authors.forename: Y
authors.initials: Y
authors.lastname: Aono
authors.forename: J
authors.initials: J
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Kinoshita
authors.forename: S
authors.initials: S
authors.lastname: Orita
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015870:Gene Expression; D016370:Gene Products, rex / Q000276:immunology*; D006168:Guinea Pigs; D015481:HTLV-I Antibodies / Q000097:blood*; D015490:HTLV-I Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014611:Vaccination
chemical_list: D004279:DNA, Viral; D016370:Gene Products, rex; D015481:HTLV-I Antibodies; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; C072341:p21X protein, Human T-lymphotropic virus 1