title: The related molecular chaperones calnexin and calreticulin differentially associate with nascent T cell antigen receptor proteins within the endoplasmic reticulum.
abstract: Assembly of the multisubunit T cell antigen receptor (TCR) complex is an intricate process requiring coordinated regulation of at least six different gene products (alpha, beta, gamma, delta, epsilon, and zeta) and the ordered pairing of partner chains within the endoplasmic reticulum (ER). To date, two proteins have been implicated as functioning as molecular chaperones in the assembly of nascent TCR proteins: calnexin, a resident ER transmembrane protein, which associates with all TCR components except zeta, and T cell receptor-associated protein, which selectively associates with CD3gammaepsilon pairs. In this study, we examined the association of calreticulin, a soluble protein with significant sequence homology to calnexin, with newly synthesized TCR proteins. Analogous to calnexin, processing of glycan chains by glucosidase enzymes was required for initial association of TCRalpha and -beta proteins with calreticulin; however, several major differences were noted regarding interaction of calnexin and calreticulin chaperones with TCR proteins. First, TCRalpha and -beta proteins showed prolonged association with calnexin molecules compared with calreticulin; interaction of TCRalpha proteins with calreticulin was particularly transient, with most calreticulin-TCRalpha protein complexes dissociating within 15 min of their initial assembly. Second, we found that, unlike calnexin, which associated with clonotypic TCRalpha and -beta proteins and invariant CD3delta and -epsilon polypeptides, calreticulin associated specifically with clonotypic TCRalpha and -beta proteins. These studies identify calreticulin as a molecular chaperone for nascent clonotypic TCRalpha and -beta proteins and demonstrate that calreticulin and calnexin differentially associate with newly synthesized TCR proteins within the ER.
authors.affiliation: Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892-1360, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Van Leeuwen
authors.forename: K P
authors.initials: KP
authors.lastname: Kearse
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D037281:Calnexin; D037282:Calreticulin; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006031:Glycosylation; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000302:isolation & purification; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013945:Thymoma; D013953:Thymus Neoplasms; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012261:Ribonucleoproteins; D013462:Sulfur Radioisotopes; C061836:antigen T cell receptor, epsilon chain; C054979:antigen T cell receptor, zeta chain; D037281:Calnexin; D008715:Methionine