Suppression by IL-10 and IL-4 of cytokine production induced by two-way autologous mixed lymphocyte reaction.
The immune response of T cells to self-HLA antigens on autologous non-T cells is known as the autologous mixed lymphocyte reaction (AMLR), and is defective in various diseases. We have investigated the effects of exogenous IL-10, a potential agent to treat various inflammatory diseases, on cytokine production induced by two-way AMLR in human peripheral blood mononuclear cells (PBMC). Effects of exogenous IL-4 were also investigated. IL-10 suppressed IL-6, IL-8, TNF-alpha and GM-CSF production when added at the beginning of culture and added 24 h after incubation. It abolished IL-1 alpha production only when added at the beginning of culture. IL-10 did not inhibit IL-3 production. The pattern of suppression by IL-10 of cytokine production induced by anti-CD3 mAb or PHA was similar to that induced by AMLR. Although IL-4 showed a largely similar pattern of inhibition when added at the beginning of culture, the level of inhibition was much lower than that of IL-10. IL-4 did not inhibit cytokine production when added 24 h after culture. These results indicate that IL-10, but not IL-4, is a potent inhibitor to cytokine production induced by two-way AMLR.
Sagawa
K
K
Department of Immunology, University School of Medicine, Fukuoka, Japan.
Mochizuki
M
M
Sugita
S
S
Nagai
K
K
Sudo
T
T
Itoh
K
K
D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D015490:HTLV-I Infections / Q000097:blood; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D016207:Cytokines; D016753:Interleukin-10; D015847:Interleukin-4