title: Stability and stabilization of Fusobacterium necrophorum hemolysin.
abstract: The stability and stabilization of the hemolytic activity of Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme were monitored over a period of four weeks using culture supernatants. The hemolytic activity was completely lost after one week at room temperature and 37 degrees C. After a two-week storage at 4 degrees C and -80 degrees C only trace activity was detected with -80 degrees C being the better of the two conditions. The addition of cysteine monohydrochloride, bovine serum albumin or Tween 80 as stabilizers, however, led to the detection of a considerable amount of the hemolytic activity in the sample stored at 4 degrees C and - 80 degrees C throughout the period investigated. The hemolytic activity appeared to be more stable in the presence of Tween 80 at -80 degrees C. Cysteine monohydrochloride was found to crystallize at - 80 degrees C and was therefore ineffective as a stabilizer at this temperature. Hemoglobin was also ineffective as a stabilizer.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: K K
authors.initials: KK
authors.lastname: Amoako
authors.forename: Y
authors.initials: Y
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Misawa
authors.forename: D L
authors.initials: DL
authors.lastname: Xu
authors.forename: T
authors.initials: T
authors.lastname: Shinjo
mesh_terms: D000418:Albumins / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D005079:Excipients / Q000494:pharmacology*; D005675:Fusobacterium necrophorum / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000187:drug effects* / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology*; D013696:Temperature
chemical_list: D000418:Albumins; D005079:Excipients; D006460:Hemolysin Proteins; D011136:Polysorbates; D003545:Cysteine