title: 4-hydroxy-2,3-trans-nonenal induces transcription and expression of aldose reductase.
abstract: Treatment of A7r5 rat vascular smooth muscle cells with 4-hydroxy-2,3-trans-nonenal (HNE) resulted in increased aldose reductase mRNA transcription. Induction was time dependent, reaching a maximum (2,3-fold) after 7 hours. An enzymatically active aldose reductase analysed by Western blotting and enzyme assays was expressed. Sorbinil, an aldose reductase inhibitor, induced a significant enhancement of HNE cytotoxicity, indicating a protective role of aldose reductase against HNE-induced A7r5 cell death. These data indicate that induction of aldose reductase by HNE may represent an important cellular defence mechanism against oxidative injury.
authors.affiliation: Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Spycher
authors.forename: S
authors.initials: S
authors.lastname: Tabataba-Vakili
authors.forename: V B
authors.initials: VB
authors.lastname: O'Donnell
authors.forename: L
authors.initials: L
authors.lastname: Palomba
authors.forename: A
authors.initials: A
authors.lastname: Azzi
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D000449:Aldehyde Reductase; C027576:4-hydroxy-2-nonenal