title: Cellular localization of Na+/MYO-inositol co-transporter mRNA in the rat brain.
abstract: The distribution of Na+/MYO-inositol co-transporter (SMIT) mRNA in the rat brain was studied by in situ hybridization histochemistry. The highest levels of SMIT mRNA were observed in the choroid plexus. Intense hybridization signals were found in the pineal gland, the area postrema, the hippocampus, the locus coeruleus, the suprachiasmatic nucleus, the olfactory bulb and the Purkinje cell and granule cell layers of the cerebellum. Low to moderate levels of labelling were detected in almost all neurones and small glia-like cells throughout the brain. These results suggest that almost all cells in the brain possess an SMIT-mediated osmotic and ionic regulatory system, and uneven densities of positive SMIT mRNA signals may reflect the differences in sensitivity of the cells to osmotic and ionic changes and also reflect differences in permeability of capillaries.
authors.affiliation: Department of Anatomy and Neuroscience, Osaka University, Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Morimura
authors.forename: A
authors.initials: A
authors.lastname: Miyai
authors.forename: A
authors.initials: A
authors.lastname: Yamauchi
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D001931:Brain Mapping; D002352:Carrier Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009457:Neuroglia / Q000737:chemistry; D009474:Neurons / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D027981:Symporters; C073884:SLC5A3 protein, human