Differential expression of the Sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants.
The involvement of the Sesbania rostrata glb3 gene promoter NICE (nodule-infected cell expression) element in root-enhanced expression of 5'-Srglb3-uidA-3'nos chimeric gene was investigated in transgenic Nicotiana tabacum plants. The full-length wild-type Srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. The expression pattern of nine selected Srglb3 promoter mutations in the NICE element was examined in transgenic tobacco plants and compared with the pattern observed in nodules of transgenic Lotus corniculatus plants. The results suggest that the highly conserved motifs in the NICE element play an important role in expression in roots of non-legume plants.
Szczyglowski
K
K
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824-1312, USA.
Potter
T
T
Stoltzfus
J
J
Fujimoto
S Y
SY
de Bruijn
F J
FJ
D001483:Base Sequence; D017124:Conserved Sequence; D007887:Fabaceae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics; D007874:Leghemoglobin / Q000235:genetics*; D018519:Meristem; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D010947:Plants, Toxic; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D007874:Leghemoglobin; D005966:Glucuronidase