title: Non-functional variants of yeast mitochondrial ATP synthase subunit 8 that assemble into the complex.
abstract: Subunit 8 (Y8) is a component of the proton channel of yeast (Saccharomyces cerevisiae) mitochondrial ATP synthase (mtATPase), whose function in the complex remains to be precisely defined. Y8 variants truncated at residue 46 (Lys47-->STP), or in which each of three conserved C-terminal amino acid residues (Arg37, Arg42 and Lys47) were substituted with isoleucine, have defects in assembly and function. The additional positive charge substitution (Gln29-->Lys) was introduced into each of the variants to determine whether functional compensation for these defects could be achieved. In the case of the (Lys47-->STP) variant, the additional positive charge restored the ability of cells to grow on non-fermentable substrate. By contrast, for the (Lys47-->Ile) variant the additional positive charge did not confer any improvement in cellular growth rate compared to that of cells expressing the Lys47-->Ile substitution alone. For the (Arg42-->Ile) and (Arg37-->Ile) variants, the presence of the Gln29-->Lys substitution failed to restore growth of host cells lacking endogenous subunit 8 on non-fermentable substrate. However, use of an in vitro assembly assay revealed that, unlike their respective parents (Arg42-->Ile or Arg37-->Ile), the (Gln29-->Lys Arg42-->Ile) and (Gln29-->Lys Arg37-->Ile) variants assemble into mtATPase. Thus we conclude that Arg42 and Arg37 have a role in mtATPase function, in addition to being required for assembly of Y8 into mtATPase.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Papakonstantinou
authors.forename: R H
authors.initials: RH
authors.lastname: Law
authors.forename: P
authors.initials: P
authors.lastname: Nagley
authors.forename: R J
authors.initials: RJ
authors.lastname: Devenish
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000251:Adenosine Triphosphatases