Analysis of steroids Part 50. Derivatization of ketosteroids for their separation and determination by capillary electrophoresis.
4-Ene-3-ketosteroids and 17-ketosteroids were quantitatively transformed into the corresponding hydrazones using Girard P and T reagents, respectively. The positively charged derivatives were separated by capillary electrophoresis. The spectrophotometric characteristics of the derivatives permitted their sensitive detection in the 230-280 nm range. The steroids investigated included nortestosterone and its phenylpropionate, norethisterone and its oenanthate, d,l-norgestrel, dehydroepiandrosterone, androstenedione and ethisterone.
Görög
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Chemical Works of Gedeon Richter Ltd., Budapest, Hungary.
Gazdag
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Kemenes-Bakos
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D000735:Androstenedione / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D001622:Betaine / Q000031:analogs & derivatives / Q000737:chemistry; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D005003:Ethisterone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D007202:Indicators and Reagents / Q000737:chemistry; D007664:Ketosteroids / Q000032:analysis / Q000302:isolation & purification*; D009277:Nandrolone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D009640:Norethindrone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D009644:Norgestrel / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D007664:Ketosteroids; D009644:Norgestrel; D001622:Betaine; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D009277:Nandrolone; C012332:Girard's reagent T; D005003:Ethisterone; D009640:Norethindrone