UVB-induced decreased resistance to Trichinella spiralis in the rat is related to impaired cellular immunity.
Our laboratory has demonstrated in preliminary experiments that UVB exposure using the Kromayer lamp can induce increased numbers of Trichinella spiralis larvae in carcasses of infected Wistar rats, without affecting specific antibody titers to this parasite. In this study, orally T. spiralis-infected Wistar rats were exposed to suberythemal doses of UVB radiation using FS40 lamps during different time periods before or after infection. A significant increase in the number of T. spiralis larvae was found in the carcasses of rats that were UVB irradiated daily for 7 consecutive days in the second week after infection. Additionally, increased numbers of larvae were also detected histologically in the tongue of rats that were exposed the first and the second week after infection. Lymphocyte stimulation assays using mesenteral lymph node cells indicated that UVB exposure also impaired the specific lymphocyte response to T. spiralis. Moreover, DTH responses to T. spiralis were severely impaired in rats that were UVB irradiated daily for 7 consecutive days in the second week after infection. Thus, these data combined with the data of the Kromayer study indicate that exposure of rats to FS40 irradiation following oral infection with T. spiralis leads to increased numbers of larvae in systemic sites and impaired T-cell immunity to the parasite.
Goettsch
W
W
National Institute for Public Health and the Environment, Bitthoven, The Netherlands.
Garssen
J
J
De Gruijl
F R
FR
Van Loveren
H
H
D000818:Animals; D007108:Immune Tolerance / Q000528:radiation effects; D007111:Immunity, Cellular / Q000528:radiation effects*; D008297:Male; D011853:Radiobiology; D051381:Rats; D017208:Rats, Wistar; D017160:Trichinella spiralis / Q000276:immunology* / Q000472:pathogenicity; D014235:Trichinellosis / Q000209:etiology; D014466:Ultraviolet Rays / Q000009:adverse effects