Selective expression of one c-myc allele in two human myeloma cell lines.
Chromosomal translocations or DNA rearrangements affecting c-myc occur in almost all murine plasmacytoma and human Burkitt's lymphoma tumors and are associated with a high incidence of exon 2 missense mutations and selective expression of the affected allele. Screening nine multiple myeloma cell lines, we identified no exon 2 missense mutations but did identify two lines with single, silent mutations in exon 1 and exon 2, respectively. Each of these informative multiple myeloma cell lines selectively expresses only one c-myc allele despite the apparent absence of chromosomal translocations or DNA rearrangements affecting c-myc.
Kuehl
W M
WM
National Cancer Institute-Navy Medical Oncology Branch, NIH, Bethesda, Maryland 20889-5105, USA. Michael_Kuehl@nih.gov
Brents
L A
LA
Chesi
M
M
Bergsagel
P L
PL
D000483:Alleles; D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology; D002461:Cell Line, Transformed; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D004854:Herpesvirus 4, Human; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm