Japanese cedar pollinosis and HLA-DP5.
Japanese cedar pollinosis is a type I allergic disease caused by Japanese cedar (Cryptomeria japonica) pollen. We investigated the association between the disease and HLA class II alleles by HLA-DNA typing using a PCR-SSOP method and found that the frequency of HLA-DP5 (DPA1*02022 and DPB1*0501) was significantly increased in the patients. To investigate whether the HLA-DP5 molecule is directly involved in the pathogenesis of the disease, Japanese cedar pollen antigen (CPAg)-specific T cell lines were established from 3 patients who possessed HLA-DP5 (DPA1*02022/ DPB1*0501). By using these CPAg-specific T cell lines and HLA class II-expressing L-cell transfectants, we found that disease-associated HLA-DP5 restricted T cells specific for CPAg existed in the patients. Furthermore, among 38 synthesized overlapping peptides spanning the entire length of one of the major Japanese cedar pollen allergens, Cry j 1, an immunodominant peptide which induced HLA-DP5 restricted Th2 was identified. These observations suggest that the HLA-DP5 may be involved, at least in part, in the pathogenesis, by helping the IgE antibody production against CPAg.
Hori
T
T
Department of Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Kamikawaji
N
N
Kimura
A
A
Sone
T
T
Komiyama
N
N
Komiyama
S
S
Sasazuki
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D002460:Cell Line; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006682:HLA-DP Antigens / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011058:Pollen / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014197:Trees
D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D006682:HLA-DP Antigens; D016056:Immunodominant Epitopes; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma