Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13.
We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment.
Carrier
A
A
Génétique Moléculaire et Biologie du Développement, CNRS-UPR 420, Villejuif, France.
Rosier
M F
MF
Guillemot
F
F
Goguel
A F
AF
Pulcini
F
F
Bernheim
A
A
Auffray
C
C
Devignes
M D
MD
D000818:Animals; D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D015183:Restriction Mapping; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers; D009414:Nerve Growth Factors