title: Kinetics of HPV11 DNA replication after infection of keratinocytes with virions.
abstract: Human papillomavirus type 11 (HPV11) normally infects keratinocytes of stratified epithelia and replicates as an episome. To untangle early events in the development of a papilloma, we have infected human keratinocytes with human papillomavirus type 11 virions and monitored replication using density labeling with bromodeoxyuridine and subsequent density centrifugation. We show that only a portion of the virus reaching the nucleus undergoes replication and that continued replication occurs without recruitment from the nonreplicating pool of virus suggesting that HPV11 replication remains confined to a small number of cells. Increasing inoculum size leads to more viral DNA reaching the nucleus but not a corresponding increase in viral replication. Subsequent papilloma development must, therefore, occur within a small subset of cells.
authors.affiliation: Department of Otolaryngology and Communicative Disorders, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, NY 11040, USA. auborn@aecom.yu.edu
authors.forename: K J
authors.initials: KJ
authors.lastname: Auborn
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: M A
authors.initials: MA
authors.lastname: Vaccariello
authors.forename: L B
authors.initials: LB
authors.lastname: Taichman
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000821:virology*; D051379:Mice; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral