Diet-induced residual formation in pigs.
Six piglets, aged 4 weeks (weaning) at the beginning of the experiment, were fed a diet lacking antioxidants (vitamin E and selenium) and enriched with oxidated cod liver oil (the peroxide value of which was 112.54 meqv/kg). Four of the experimental pigs were killed for necropsy at the age of 2 months and the remaining two pigs at the age of 3 months. Two piglets from the same litter served as controls. Myodegeneration of the skeletal muscles was induced in the experimental group. The livers were pale and mottled. Light microscopy revealed degenerative alterations in the heart and skeletal muscle and infiltrations by lipidic substances. The ultrastructure of some lipidic droplets was analogous to those of ceroid and lipofuscin. Peroxisomes were frequent in degenerating hepatocytes. In order to elucidate the lysosomal involvement in the residual formation, light and electron microscopical immunocytochemistry using copper-zinc superoxide dismutase (CuZnSOD), was applied. Probably zinc was recruited into the antioxidant protection concluding upon the increased erythrocyte superoxide dismutase (E-SOD) activity.
de Gritz
B G
BG
Pathological Anatomy, College of Veterinary Medicine, Helsinki, Finland.
Rahko
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D002566:Ceroid / Q000378:metabolism*; D046508:Culture Techniques; D004032:Diet / Q000009:adverse effects*; D007150:Immunohistochemistry; D008062:Lipofuscin / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D018384:Oxidative Stress / Q000502:physiology; D012643:Selenium / Q000172:deficiency; D013552:Swine; D014811:Vitamin E Deficiency
D000975:Antioxidants; D002566:Ceroid; D008062:Lipofuscin; D012643:Selenium