title: Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae.
abstract: Two cis-acting elements have been identified that act synergistically to regulate expression of the glucose-repressed alcohol dehydrogenase 2 (ADH2) gene. UAS1 is bound by the trans-activator Adr1p. UAS2 is thought to be the binding site for an unidentified regulatory protein. A genetic selection based on a UAS2-dependent ADH2 reporter was devised to isolate genes capable of activating UAS2-dependent transcription. One set of UAS2-dependent genes contained SPT6/CRE2/SSN20. Multicopy SPT6 caused improper expression of chromosomal ADH2. A second set of UAS2-dependent clones contained a previously uncharacterized open reading frame designated MEU1 (Multicopy Enhancer of UAS2). A frame shift mutation in MEU1 abolished its ability to activate UAS2-dependent gene expression. Multicopy MEU1 expression suppressed the constitutive ADH2 expression caused by cre2-1. Disruption of MEU1 reduced endogenous ADH2 expression about twofold but had no effect on cell viability or growth. No homologues of MEU1 were identified by low-stringency Southern hybridization of yeast genomic DNA, and no significant homologues were found in the sequence data bases. A MEU1/beta-gal fusion protein was not localized to a particular region of the cell. MEU1 is linked to PPR1 on chromosome XII.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle 98195, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Donoviel
authors.forename: E T
authors.initials: ET
authors.lastname: Young
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D019747:Aldose-Ketose Isomerases; D001483:Base Sequence; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005947:Glucose / Q000378:metabolism; D056488:Histone Chaperones; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000235:genetics*; D035602:Transcriptional Elongation Factors; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C065456:SPT6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D035602:Transcriptional Elongation Factors; D000426:Alcohol Dehydrogenase; D001616:beta-Galactosidase; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae; D005947:Glucose