title: Visualization of gene expression in living adult Drosophila.
abstract: To identify genes involved in the patterning of adult structures, Gal4-UAS (upstream activating site) technology was used to visualize patterns of gene expression directly in living flies. A large number of Gal4 insertion lines were generated and their expression patterns were studied. In addition to identifying several characterized developmental genes, the approach revealed previously unsuspected genetic subdivisions of the thorax, which may control the disposition of pattern elements. The boundary between two of these domains coincides with localized expression of the signaling molecule wingless.
authors.affiliation: Centro de Biología Molecular, Universidad Autónoma de Madrid, Consejo Superior de Investigaciónes Científicas, Madrid 28049, Spain. gmorata@mvax.cbm.uam.es
authors.forename: M
authors.initials: M
authors.lastname: Calleja
authors.forename: E
authors.initials: E
authors.lastname: Moreno
authors.forename: S
authors.initials: S
authors.lastname: Pelaz
authors.forename: G
authors.initials: G
authors.lastname: Morata
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D005656:Fungal Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005796:Genes; D050437:Genes, Developmental; D017344:Genes, Insect; D011518:Proto-Oncogene Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013909:Thorax / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics; D051155:Wnt1 Protein
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011518:Proto-Oncogene Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C074622:wg protein, Drosophila