title: Identification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci.
abstract: While attempting to identify genes and their corresponding antigens that could be used to improve the current methods of diagnosing Chlamydia psittaci infection which causes enzootic abortion in ewes, two candidate clones were isolated from a lambda gt 11 genomic DNA expression library of ovine abortion subtype (strain S26/3) C. psittaci. These clones contained fragments of a gene coding for a group of three chlamydial proteins of approximately 90 kDa which appeared as major immunogens by immunoblotting experiments, indicating their potential as diagnostic or possibly protective antigens. Southern blotting of S26/3 genomic DNA using the two clones as probes identified a family of three or four genes. These represent the first example of protein gene duplication reported in Chlamydia.
authors.affiliation: Moredun Research Institute, Edinburgh, UK.
authors.forename: D
authors.initials: D
authors.lastname: Longbottom
authors.forename: M
authors.initials: M
authors.lastname: Russell
authors.forename: G E
authors.initials: GE
authors.lastname: Jones
authors.forename: F A
authors.initials: FA
authors.lastname: Lainson
authors.forename: A J
authors.initials: AJ
authors.lastname: Herring
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D002691:Chlamydophila psittaci / Q000235:genetics*; D003001:Cloning, Molecular; D015723:Gene Library; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D001425:Bacterial Outer Membrane Proteins