The existence of thyroid hormone responsive element, TRE, was confirmed in the first intron of rat alpha 1-acid glycoprotein gene.
A set of acute-phase proteins was found to have been induced in rat after triiodothyronine (T3) treatment. To study the mechanism of gene activation by T3, the rat gene of alpha 1-acid glycoprotein (AGP), one of the major acute-phase proteins, was cloned and analyzed. CAT assay identified a thyroid hormone responsive element (TRE) in the first intron of the AGP gene and showed that mutations introduced into the TRE region destroyed the TRE activity. Gel shift assay and methylation interference assay showed that the thyroid hormone receptor bound to the TRE. The TRE identified here had a palindromic structure and was highly homologous to other known TREs.
Matsukawa
T
T
Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa.
Kitagawa
M
M
Katayama
K
K
Nagai
Y
Y
Hayashi
T
T
Yasuda
H
H
Ohba
Y
Y
D000209:Acute-Phase Proteins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000235:genetics; D007438:Introns / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D009961:Orosomucoid / Q000235:genetics* / Q000302:isolation & purification; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D017421:Sequence Analysis; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology*
D000209:Acute-Phase Proteins; D009961:Orosomucoid; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D004247:DNA