title: A t(3;8) chromosomal translocation associated with hepatitis B virus intergration involves the carboxypeptidase N locus.
abstract: Integrated hepatitis B virus (HBV) DNA is found in the great majority of human hepatocellular carcinomas, suggesting that these viral integrations may be implicated in liver oncogenesis. Besides the insertional mutagenesis characterized in a few selected cases and the contribution of viral transactivators to cell transformation to malignancy, HBV has been shown to generate gross chromosomal rearrangements potentially involved in carcinogenesis. Here, we report a t(3;8) chromosomal translocation present in a hepatocellular carcinoma developed in noncirrhotic liver tissue. One side of the translocation, in 8p23, is shown to be in the vicinity of the carboxypeptidase N gene, a locus that is heavily transcribed in liver tissue and frequently deleted in hepatocellular carcinomas and other epithelial tumors. The other side of the translocation, in 3q27-29, is widely implicated in several types of translocations occurring in different malignancies, such as large-cell lymphomas. The present data strongly support a model in which HBV-induced chromosomal rearrangements play a key role during multistep liver oncogenesis.
authors.affiliation: Unité de Recombinaison et Expression Génétique, Institut National dela Santé et de la Recherche Médicale (INSERM) U163, Institut Pasteur,Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Pineau
authors.forename: A
authors.initials: A
authors.lastname: Marchio
authors.forename: B
authors.initials: B
authors.lastname: Terris
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: Z X
authors.initials: ZX
authors.lastname: Tu
authors.forename: P
authors.initials: P
authors.lastname: Tiollais
authors.forename: A
authors.initials: A
authors.lastname: Dejean
mesh_terms: D000328:Adult; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000821:virology*; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000821:virology*; D001121:Lysine Carboxypeptidase / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014178:Translocation, Genetic; D016662:Virus Integration
chemical_list: D001121:Lysine Carboxypeptidase