Effect of XPA gene mutations on UV-induced immunostaining of PCNA in fibroblasts from xeroderma pigmentosum group A patients.
We have investigated the relationship between XPA gene mutations and PCNA complex formation in the nucleotide excision repair (NER) process utilizing cells derived from various xeroderma pigmentosum group A (XP-A) patients. The PCNA complex formation was detected by PCNA immunostaining following methanol fixation. Results indicated that UV-induced PCNA staining at early stages was well correlated to the function of XPA protein and provided evidence that XPA protein-related recognition step was tightly linked to PCNA-associated events in the NER process in vivo.
Miura
M
M
Department of Dental Radiology and Radiation Research, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Sasaki
T
T
D002460:Cell Line; D003062:Codon; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005091:Exons; D005347:Fibroblasts; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D007150:Immunohistochemistry; D009154:Mutation; D017354:Point Mutation; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000096:biosynthesis*; D017384:Sequence Deletion; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics*; D051760:Xeroderma Pigmentosum Group A Protein
D003062:Codon; D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; C497314:XPA protein, human; D051760:Xeroderma Pigmentosum Group A Protein; D004247:DNA