Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol.
4-Iodophenylboronic acid has been synthesized and shown to be a potent enhancer of the chemiluminescent horseradish peroxidase (Type VI-A)-catalyzed oxidation of luminol and isoluminol. The enhancer was effective (>100-fold enhancement) in the concentration range 10-1500 microM. Light emission in the presence of the enhancer peaked at 5-10 min after initiation of the reaction and then decayed very slowly. 4-Iodophenylboronic acid also enhanced reactions catalyzed by horseradish peroxidase Type IX. The detection limit for Type VI-A horseradish peroxidase was 509 amol, and optimum signal enhancement was obtained at 769 microM compared to 154 microM for 4-iodophenol under the same conditions.
Kricka
L J
LJ
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, Pennsylvania, 19104, USA.
Cooper
M
M
Ji
X
X
D015153:Blotting, Western / Q000379:methods; D001896:Boron Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000138:chemical synthesis* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives / Q000378:metabolism; D010084:Oxidation-Reduction; D014168:Transferrin / Q000032:analysis
D001896:Boron Compounds; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D014168:Transferrin; C032487:isoluminol; C080454:4-iodophenylboronic acid; C050181:4-iodophenol; C036647:4-bromophenylboric acid; D008165:Luminol; D006735:Horseradish Peroxidase