Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury.
Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 +/- 4.4% decrease in tissue resistance (Rt). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in Rt to 10.2 +/- 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.
Rao
R
R
Department of Pediatrics, Medical University of South Carolina, Charleston 29403, USA. raor@ped2.mhs.musc.edu
Porreca
F
F
D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017830:Octoxynol / Q000633:toxicity*
D004815:Epidermal Growth Factor; D017830:Octoxynol