Effect of topically applied sulphur mustard on antioxidant enzymes in blood cells and body tissues of rats.
The effect of sulphur mustard (0.5 LD50, percutaneous) on antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)) in blood cells (erythrocytes (RBC), leucocytes (WBC) and platelets) and body tissues (liver, kidney, spleen and brain) of rats has been investigated 24 h post exposure. The SOD activity was significantly decreased in WBC, platelets, spleen and brain as compared to control. The CAT activity was significantly inhibited in RBC, WBC and spleen as compared to control. The GSH-Px activity was signficantly depressed in WBC, spleen and liver as compared to control. It is concluded that sulphur mustard at a sublethal dose inhibited antioxidant enzyme activities in WBC and spleen. Thus, antioxidant enzymes in lymphatic tissues may be used as suitable models for assessing mustard toxicity. The study suggests the formation of reactive oxygen species in sulphur mustard intoxication.
Husain
K
K
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Dube
S N
SN
Sugendran
K
K
Singh
R
R
Das Gupta
S
S
Somani
S M
SM
D000287:Administration, Topical; D000818:Animals; D001773:Blood Cells / Q000187:drug effects* / Q000201:enzymology*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009151:Mustard Gas / Q000008:administration & dosage / Q000633:toxicity*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors* / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000187:drug effects; D014781:Viscera / Q000187:drug effects* / Q000201:enzymology
D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D009151:Mustard Gas