A rapid polarographic immunoassay based on the anodic current of metal labeling.
A heterogeneous voltammetric immunoassay of human serum albumin (HSA) based on the anodic current of metal labeling was studied. HSA was covalently coupled to diethylenetriaminepentaacetic anhydride and labeled with In3+. A heterogeneous competitive immunochemical reaction was carried out in the wells of a microtiter plate. The In3+ label was released in 1 M HCl solution and determined by simplified anodic stripping neopolarography. HSA could be detected in the concentration range of 4.0-50 microg ml-1. The method was used to determine HSA in serum specimens, and the results were in good agreement with those obtained with dye binding spectrophotometry.
Qiu
J
J
Institute of Electroanalytical Chemistry, Northwest University, Xian, 710069, People's Republic of China.
Song
J
J
D002614:Chelating Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D007204:Indium; D004369:Pentetic Acid; D011048:Polarography / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis*
D002614:Chelating Agents; D012709:Serum Albumin; D007204:Indium; D004369:Pentetic Acid