Interleaflet clear space is reduced in the membrane of COP I and COP II-coated buds/vesicles.
Intracellular transfers between membrane-bound compartments occur through vesicles that bud from a donor compartment to fuse subsequently with an acceptor membrane. We report that the membrane that delimits COP I or COP II-coated buds/vesicles from the endoplasmic reticulum and the Golgi complex has a thinner interleaflet clear space as compared with the surrounding, noncoated parental membrane. This change is compatible with a compositional change of the membrane bilayer during the budding process.
Orci
L
L
Department of Morphology, University of Geneva Medical Center, Switzerland.
Schekman
R
R
Perrelet
A
A
D022181:COP-Coated Vesicles; D018699:Coated Vesicles / Q000737:chemistry / Q000648:ultrastructure*; D016616:Cryoultramicrotomy; D003720:Densitometry; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000302:isolation & purification*; D019204:GTP-Binding Proteins / Q000302:isolation & purification*; D020690:GTPase-Activating Proteins; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000302:isolation & purification*; D016253:Microscopy, Immunoelectron; D020559:Monomeric GTP-Binding Proteins; D028861:Nuclear Pore Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins
D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; C079620:SEC13 protein, S cerevisiae; C079037:SEC23 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae