A rapid, colourimetric assay for cytotoxin activity in Campylobacter jejuni.
Cell extracts and culture supernates of Campylobacter jejuni NCTC 11168 and three isolates from faecal samples from patients with enteritis were tested for cytotoxic activity on HeLa and Vero cells using a sensitive and rapid dye reduction assay which represents a simple assay for cytotoxin activity that can be assessed visually or spectrophotometrically in the wells of microplates. The assay was as sensitive as trypan blue exclusion and did not require the use of radioisotopes. A low level of cytotoxin activity, compared to that produced by a control verotoxin 2-producing Escherichia coli strain, was detected in cell extracts of all four strains, but no activity was detected in culture supernates. Production of an enterotoxin was evaluated by reverse passive latex agglutination with anti-cholera toxin antibody, a procedure which also represents a rapid and simple assay for this toxin. No enterotoxin activity was detected in cell extracts or culture supernates from any of the isolates.
Coote
J G
JG
Institute of Biomedical and Life Sciences, University of Glasgow, UK.
Arain
T
T
D000818:Animals; D016123:Campylobacter jejuni / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003124:Colorimetry / Q000379:methods*; D003603:Cytotoxins / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay; D007841:Latex Fixation Tests; D013778:Tetrazolium Salts; D013844:Thiazoles; D014709:Vero Cells
D003603:Cytotoxins; D004768:Enterotoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue