2D-PAGE examination of mRNA populations from Penicillium freii mutants deficient in xanthomegnin biosynthesis.
Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Specific translation products were absent in mutants and natural isolates unable to produce xanthomegnin metabolites. One mutant (TSM 73) did not produce several of these translation products, indicating that a mutation in a regulatory gene involved in xanthomegnin production had occurred.
Nicolaisen
M
M
Biotechnological Institute, Technical University of Denmark, Lyngby, Denmark.
Sandal
T
T
Frisvad
J C
JC
Rossen
L
L
D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D015966:Gene Expression Regulation, Fungal; D014644:Genetic Variation; D006912:Hydroxyquinolines / Q000378:metabolism; D016296:Mutagenesis / Q000528:radiation effects; D009285:Naphthoquinones / Q000378:metabolism*; D010407:Penicillium / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D019820:Xanthine; D014970:Xanthines / Q000378:metabolism
D005656:Fungal Proteins; D006912:Hydroxyquinolines; D009285:Naphthoquinones; D012333:RNA, Messenger; D014970:Xanthines; C009688:xanthomegnin; D019820:Xanthine; C009673:viridicatin; C013735:viomellein