In vitro microsome-mediated aflatoxin B1-DNA binding and its inhibition by cytosol of various organs of the hamster and quail.
We studied the in vitro activation of aflatoxin B1 (B1) by microsomes and its inactivation by the cytosol of various quail and hamster organs, using B1-DNA binding as an index. The microsomal activity of the liver to bind B1 to DNA was not largely different between the two species and was higher than that of the other organs examined in either species. The microsomal activity of the kidney and lung was very low in the quail compared with the hamster, indicating the very small contribution of the lung and kidney microsomes to the activation of B1 in birds. Only the hamster liver cytosol showed strong inhibition of microsome-mediated B1-DNA binding.
Kono
M
M
Department of Biomedical Food Research, National Institute of Health, Shinjuku-ku, Tokyo, Japan.
Kumagai
S
S
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D003370:Coturnix; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D005260:Female; D008658:Inactivation, Metabolic; D008297:Male; D008647:Mesocricetus; D008861:Microsomes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D013045:Species Specificity; D014018:Tissue Distribution
D002273:Carcinogens; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1