Photodynamic efficiency of macrophage activity using a photosensitizer, lumin, with near-IR laser light for photoimmunotherapy of a cancer.
This study was carried out to determine the efficacy of photoimmunotherapy using a photosensitizer, lumin, and near-IR laser light irradiation for cancer therapy. Lumin is a potent cell-activating agent and a photosensitizer characterized by typical absorption peaks at 670 and 770 nm. To clarify that ingestion activity is enhanced by lumin administration with different light irradiation, He-Ne laser light (632.8 nm) and near-IR laser light (780 nm) were used. The results showed that a small dose of lumin (5 ng/ml) with near-IR laser light dramatically enhanced the ingestion activity. A human lung cancer grafted onto nude mice was used in this study. After the tumor hypertrophied, lumin was injected into it six times. Then, it was exposed to near-IR laser light (2 mW, 1 min) every other day for 2 weeks. The whole tumor was strongly cicatrized by collagen fiber from the stroma. It was removed 15 weeks after the treatment. Immunological function was investigated using a two-color flow cytometric assay method based on immunofluorescence labeling with specific monoclonal antibodies of 10(4) cell samples harvested from mouse peripheral blood. At 40 weeks, the ratio of T cells to B cells in untreated mice was 0.23, whereas that in treated mice was 0.58. These results demonstrated that photoimmunotherapy with lumin administration and laser light irradiation is a reliable method of cancer therapy.
Mito
K
K
Kawasaki College of Allied Health Professions, Kurashiki, Japan.
D000230:Adenocarcinoma / Q000628:therapy*; D000818:Animals; D002460:Cell Line; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007167:Immunotherapy; D007259:Infrared Rays; D007834:Lasers; D008175:Lung Neoplasms / Q000628:therapy*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011806:Quinolinium Compounds / Q000494:pharmacology*; D014183:Transplantation, Heterologous
D007166:Immunosuppressive Agents; D017319:Photosensitizing Agents; D011806:Quinolinium Compounds; C052194:lumin