In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants.
Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed J774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.
Khelef
N
N
Laboratoire des Bordetella, Institut Pasteur, Paris, France.
DeShazer
D
D
Friedman
R L
RL
Guiso
N
N
D037361:Adenylate Cyclase Toxin; D000818:Animals; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D001885:Bordetella Infections / Q000235:genetics / Q000382:microbiology; D001886:Bordetella pertussis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D002374:Catalase / Q000235:genetics*; D002478:Cells, Cultured; D015151:Immunoblotting; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D010269:Paraquat / Q000494:pharmacology; D011498:Protein Precursors / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000235:genetics*; D014774:Virulence; D010566:Virulence Factors, Bordetella
D037361:Adenylate Cyclase Toxin; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011498:Protein Precursors; D010566:Virulence Factors, Bordetella; C068929:pertactin; D002374:Catalase; D013482:Superoxide Dismutase; D010269:Paraquat