title: Comparison of plasmid DNA preparation methods for direct gene transfer and genetic immunization.
abstract: Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.
authors.affiliation: Loeb Medical Research Institute, Ottawa Civic Hospital, ON, Canada. hdavis@civich.ottawa.on.ca
authors.forename: H L
authors.initials: HL
authors.lastname: Davis
authors.forename: M
authors.initials: M
authors.lastname: Schleef
authors.forename: P
authors.initials: P
authors.lastname: Moritz
authors.forename: M
authors.initials: M
authors.lastname: Mancini
authors.forename: J
authors.initials: J
authors.lastname: Schorr
authors.forename: R G
authors.initials: RG
authors.lastname: Whalen
mesh_terms: D000818:Animals; D000838:Anions; D002586:Cesium; D002712:Chlorides; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D007114:Immunization; D008156:Luciferases / Q000235:genetics; D051379:Mice; D018482:Muscle, Skeletal; D010957:Plasmids / Q000235:genetics*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000838:Anions; D002712:Chlorides; D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens; D019444:Vaccines, DNA; D002586:Cesium; D004247:DNA; D008156:Luciferases; C028019:cesium chloride