Microiontophoresis of kanamycin from micropipettes in vitro.
1. Currents ranging between 10 and 50 nA were passed during ten minutes through 0,02 M kanamycin (KM) filled micropipettes with tips submerged in 25 microliters of KCl 0,15 M. 2. The amount of KM released was measured by radioimmunoassay. 3. It was found that the amount of kanamycin released could be computed by the equation y = 0,16x + 0,59; where "x" stands for charge passed in micro Coulombs and "y" stands for the amount released in nanograms. 4. When Faraday's law was used to fit the experimental data, it was found that the electrodes behave in an acceptable linear fashion. The range of the transport number for KM was 0,089 to 0,142 for six electrodes.
Echeverria
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EL
Departamento de Ciencias Básicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Gonzalez
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LQ
D007478:Iontophoresis / Q000295:instrumentation / Q000379:methods*; D007612:Kanamycin / Q000008:administration & dosage*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
D007612:Kanamycin