Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.
Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.
da Silva
A J
AJ
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.
Schwartz
D A
DA
Visvesvara
G S
GS
de Moura
H
H
Slemenda
S B
SB
Pieniazek
N J
NJ
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D016816:Microsporida / Q000235:genetics* / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D016053:RNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016053:RNA, Protozoan; D012335:RNA, Ribosomal