Equivalent parental distribution of frequently lost alleles and biallelic expression of the H19 gene in human testicular germ cell tumors.
Epigenetic alterations such as genomic imprinting might play an important role in human tumorigenesis, in addition to specific genetic alterations. To clarify the role of genetic and/or epigenetic alterations in the tumorigenesis of testicular germ cell tumors (GCTs), we analyzed 40 primary and 3 metastatic testicular GCTs with regard to specific chromosomal losses and their parental origin. A high incidence of loss of heterozygosity (LOH) was demonstrated on chromosomes 1p, 3p, 11p, and 17p: 9/19 (47%), 18/39 (46%), 13/40 (33%) and 20/36 (56%), respectively. However, there was no correlation between the frequency of LOH on any chromosome and clinicopathological features. Regarding the parental origin of the lost allele at these chromosomes, preferential loss was not demonstrated in this study. To clarify the imprinting status in GCTs, we analyzed the allele-specific expression of the H19 gene, which is paternally imprinted on chromosome 11p. All of 11 tumors without LOH at this locus showed biallelic expression of H19. Based on previous work demonstrating the biallelic expression of H19 in primordial germ cells and spermatogonia in the mouse germ line, these results suggest that the biallelic expression of H19 in testicular GCTs reflects the characteristics of the original germ cells in which the imprinting marking has been erased and not established, rather than loss of imprinting during tumorigenesis. It is also possible that a failure to re-establish the imprinting might be an initial event which leads to testicular GCTs.
Mishina
M
M
Department of Urology, Faculty of Medicine, Kyoto University.
Ogawa
O
O
Kinoshita
H
H
Oka
H
H
Okumura
K
K
Mitsumori
K
K
Kakehi
Y
Y
Reeve
A E
AE
Yoshida
O
O
D000483:Alleles; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D018392:Genomic Imprinting; D018237:Germinoma / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D018239:Seminoma / Q000235:genetics; D013736:Testicular Neoplasms / Q000235:genetics*
C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated