Activation of the human homologue of the Drosophila sina gene in apoptosis and tumor suppression.
Developmentally regulated genes in Drosophila, which are conserved through evolution, are potential candidates for key functions in biological processes such as cell cycle, programmed cell death, and cancer. We report cloning and characterization of the human homologue of the Drosophila seven in absentia gene (HUMSIAH), which codes for a 282 amino acids putative zinc finger protein. HUMSIAH is localized on human chromosome 16q12-q13. This gene is activated during the physiological program of cell death in the intestinal epithelium. Moreover, human cancer-derived cells selected for suppression of their tumorigenic phenotype exhibit constitutively elevated levels of HUMSIAH mRNA. A similar pattern of expression is also displayed by the p21waf1. These results suggest that mammalian seven in absentia gene, which is a target for activation by p53, may play a role in apoptosis and tumor suppression.
Nemani
M
M
Fondation Jean Dausset, Centre d'Etudes du Polymorphisme Humain (CEPH), Paris, France.
Linares-Cruz
G
G
Bruzzoni-Giovanelli
H
H
Roperch
J P
JP
Tuynder
M
M
Bougueleret
L
L
Cherif
D
D
Medhioub
M
M
Pasturaud
P
P
Alvaro
V
V
der Sarkissan
H
H
Cazes
L
L
Le Paslier
D
D
Le Gall
I
I
Israeli
D
D
Dausset
J
J
Sigaux
F
F
Chumakov
I
I
Oren
M
M
Calvo
F
F
Amson
R B
RB
Cohen
D
D
Telerman
A
A
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers; D000098563:Seven in Absentia Proteins
D018076:DNA, Complementary; D009687:Nuclear Proteins; D011506:Proteins; D044767:Ubiquitin-Protein Ligases; D000098563:Seven in Absentia Proteins