Influenza A and Sendai viruses preferentially bind to fucosylated gangliosides with linear poly-N-acetyllactosaminyl chains from human granulocytes.
Influenza A and Sendai viruses are known to bind to various extent to neolacto-series gangliosides IV3Neu5Ac-nLcOse4Cer, IV6Neu5Ac-nLcOse4Cer, and VI3Neu5Ac-nLcOse6Cer, which are the dominant gangliosides of human granulocytes. Recently, minor gangliosides of granulocytes were characterized and found to express sialyl Lewis(x) and VIM-2 epitopes. These long chain linear monosialogangliosides with nLcOse8, and nLcOse10, cores, carrying one to three fucoses, are shown in this study to bind with strong avidity to influenza A/PR/8/34 (H1N1), A/X-31 (H3N2), and Sendai virus (Z-strain) using the overlay technique. These and recent data from other groups imply that selectins and virus hemagglutinins are capable of competing with lipid bound sialyl Lewis(x) and VIM-2 epitopes on myeloid cells during inflammatory reactions.
Müthing
J
J
Institute of Cell Culture Technology, University of Bielefeld, Germany.
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005643:Fucose / Q000031:analogs & derivatives; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000378:metabolism; D009975:Orthomyxoviridae / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D018108:Respirovirus / Q000378:metabolism*; D019087:Selectins / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D009844:Oligosaccharides; D011134:Polysaccharides; D019087:Selectins; D000080506:Sialyl Lewis X Antigen; C070946:VIM-2 determinant; D005643:Fucose; C037199:poly-N-acetyllactosamine