Glycoprotein A is the immunodominant antigen of Pneumocystis carinii in mice following immunization.
A rapid and simple dual enzyme-linked immunosorbent assay was developed to quantitate the relative frequency of monoclonal antibodies produced against Pneumocystis carinii glycoprotein A (gpA) or other antigens of P. carinii after immunization with whole organisms. In BALB/cj mice, P. carinii gpA was clearly the immunodominant antigen, with a quarter of all hybridomas secreting antibody directed against this molecule.
Gigliotti
F
F
University of Rochester Medical Center, Rochester, NY 14642, USA.
McCool
T
T
D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology*; D005656:Fungal Proteins / Q000276:immunology*; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011010:Pneumocystis / Q000276:immunology*
D000946:Antigens, Fungal; D005656:Fungal Proteins; D016056:Immunodominant Epitopes; D008562:Membrane Glycoproteins; C077556:GPA protein, Pneumocystis carinii sp.