title: Spatial proximity of Cys113, Cys172, and Cys422 in the metalloactivation domain of the ArsA ATPase.
abstract: ArsA ATPase activity is allosterically activated by salts of the semimetal arsenic or antimony. Activation is associated with the presence of three cysteine residues in ArsA: Cys113, Cys172, and Cys422. To determine the distance between cysteine residues, wild type ArsA and ArsA proteins with cysteine to serine substitutions were treated with the bifunctional alkylating agent dibromobimane, which reacts with thiol pairs within 3-6 A of each other to form a fluorescent adduct. ArsA proteins in which single cysteine residues were altered by site-directed mutagenesis still formed fluorescent adducts. Proteins in which two of the three cysteine residues were substituted did not form fluorescent adducts. These results demonstrate that Cys113, Cys172, and Cys422 are in close proximity of each other. We propose a model in which As(III) or Sb(III) interacts with these three cysteines in a trigonal pyramidal geometry, forming a novel soft metal-thiol cage.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: H
authors.initials: H
authors.lastname: Bhattacharjee
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053501:Arsenite Transporting ATPases; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry*; D004789:Enzyme Activation; D016623:Ion Pumps; D009097:Multienzyme Complexes; D016297:Mutagenesis, Site-Directed
chemical_list: D001643:Bridged Bicyclo Compounds; D016623:Ion Pumps; D009097:Multienzyme Complexes; C037094:dibromobimane; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; D003545:Cysteine