DNA cleavage by an alpha, 3-dehydrotoluene precursor conjugated to a minor groove binding element.
The (Z)-allene-ene-yne 10, a precursor to a reactive alpha,3-dehydrotoluene biradical, was covalently linked to a minor groove binding element, and the resultant conjugate (9) was shown to bind and cleave DNA with sequence selectivity.
Myers
A G
AG
Arnold and Mabel Beckman Laboratories of Chemical Synthesis, California Institute of Technology, Pasadena.
Parrish
C A
CA
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003500:Cyclization; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004214:Distamycins / Q000737:chemistry / Q000378:metabolism; D004229:Dithiothreitol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005609:Free Radicals / Q000737:chemistry; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000737:chemistry*; D014050:Toluene / Q000031:analogs & derivatives* / Q000737:chemistry*
D004214:Distamycins; D005609:Free Radicals; D010761:Phosphorus Radioisotopes; D017382:Reactive Oxygen Species; D014050:Toluene; D004247:DNA; D004262:DNA Restriction Enzymes; D004229:Dithiothreitol