title: Insulin modulation of newly synthesized apolipoproteins B-100 and B-48 in human fetal intestine: gene expression and mRNA editing are not involved.
abstract: We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion. When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin. HDL lipid content, apo A-1 synthesis and RNA level were unaffected by insulin. Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.
authors.affiliation: Department of Nutrition, Hôpital Sainte-Justine, Université de Montréal, Québec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Levy
authors.forename: D
authors.initials: D
authors.lastname: Sinnett
authors.forename: L
authors.initials: L
authors.lastname: Thibault
authors.forename: T D
authors.initials: TD
authors.lastname: Nguyen
authors.forename: E
authors.initials: E
authors.lastname: Delvin
authors.forename: D
authors.initials: D
authors.lastname: Ménard
mesh_terms: D000028:Abortion, Induced; D016632:Apolipoprotein A-I / Q000096:biosynthesis; D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000096:biosynthesis*; D017931:DNA Primers; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007583:Jejunum / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000096:biosynthesis; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017393:RNA Editing
chemical_list: D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D017931:DNA Primers; D007328:Insulin; D008074:Lipoproteins