Development of a new bioartificial liver module filled with porcine hepatocytes immobilized on non-woven fabric.
We developed a new bioartificial liver module, a non-woven fabric (NWF) module, which was filled with hepatocytes immobilized on non-woven polyester fabric, and compared its efficacy with a suspension culture vessel. Porcine hepatocytes were isolated from whole pig liver, which formed spheroids in a 1L suspension culture vessel. Some of these spheroids were allowed to construct a 50 mL NWF module by inoculation onto 12 sheets of disk-shaped non-woven fabric then stuffed into a 50 mL column. The remaining spheroids were transferred to a 100 mL spinner flask and placed in suspension. The 50 mL NWF module maintained a much better metabolic and synthetic function than the 100 mL suspension culture vessel throughout the study. Based on these results, we developed a 200 mL radial-flow-type NWF module. This module filled with single hepatocytes showed promising efficiency.
Naruse
K
K
First Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
Sakai
Y
Y
Nagashima
I
I
Jiang
G X
GX
Suzuki
M
M
Muto
T
T
D000818:Animals; D002469:Cell Separation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D017114:Liver Failure, Acute / Q000628:therapy; D019164:Liver, Artificial; D008854:Microscopy, Electron; D011091:Polyesters / Q000737:chemistry; D018874:Spheroids, Cellular / Q000166:cytology; D013535:Suspensions; D013552:Swine
D011091:Polyesters; D013535:Suspensions