Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor.
Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.
Karow
J
J
Department of Biochemistry, University of Oxford, U.K.
Hudson
K R
KR
Hall
M A
MA
Vernallis
A B
AB
Taylor
J A
JA
Gossler
A
A
Heath
J K
JK
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D050822:Cytokine Receptor gp130; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D006801:Humans; D017370:Interleukin-11 / Q000378:metabolism / Q000494:pharmacology*; D053703:Interleukin-11 Receptor alpha Subunit; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D016133:Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000187:drug effects / Q000502:physiology*; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
D015703:Antigens, CD; C508428:IL11RA protein, human; C494199:IL6ST protein, human; C508429:Il11ra1 protein, mouse; C494200:Il6st protein, mouse; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins; D050822:Cytokine Receptor gp130