title: Expression of CD10/neutral endopeptidase in normal and malignant tissues of the human stomach and colon.
abstract: The expression of common acute lymphoblastic leukemia antigen (CALLA; CD10), which is identical to neutral endopeptidase (NEP, EC3.424.11), was examined in the malignant and adjacent noninvaded tissues of the human stomach and colon (n = 27). All of 27 normal and 18 well or moderately differentiated adenocarcioma tissue specimens were positive for monoclonal antibody (mAb) NL-1 against CD10/NEP, whereas the expression level was clearly decreased in all of 9 specimens of poorly differentiated adenocarcinoma. In addition, all of 7 gastric or colorectal carcinoma cell lines tested showed decreased expression of CD10/NEP. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the crude antigen J5 from the normal colon tissue lysate by mAb J5 detected a single band of approximately 100 kDa that was consistent with that of NALM-6 cells used as a positive control. These findings suggest that CD10/NEP is expressed in normal epithelial cells of the human stomach and colon, whereas the expression level is decreased in the poorly differentiated type of adenocarcinoma.
authors.affiliation: First Department of Internal Medicine, Sapporo Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: F
authors.initials: F
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Hinoda
authors.forename: Y
authors.initials: Y
authors.lastname: Ohe
authors.forename: N
authors.initials: N
authors.lastname: Nakagawa
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.forename: A
authors.initials: A
authors.lastname: Yachi
authors.forename: K
authors.initials: K
authors.lastname: Imai
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015260:Neprilysin / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D015260:Neprilysin