Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.
Baskaran
N
N
Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
Kandpal
R P
RP
Bhargava
A K
AK
Glynn
M W
MW
Bale
A
A
Weissman
S M
SM
D001482:Base Composition; D001622:Betaine; D002352:Carrier Proteins / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide; D005260:Female; D005600:Fragile X Syndrome / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D027702:Organic Cation Transporter 1; D016133:Polymerase Chain Reaction / Q000379:methods*; D011990:Receptors, Transferrin / Q000235:genetics; D013698:Templates, Genetic; D018911:Trinucleotide Repeats
D002352:Carrier Proteins; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1; D011990:Receptors, Transferrin; D001622:Betaine; D004247:DNA; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide