Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14.
Cofilin is a widely-distributed, intracellular, actin binding protein which is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. We have cloned a non-muscle-type cofilin (CFL1) from a human promyelocytic cDNA library and mapped this to human chromosome 11 by PCR amplification of 3' untranslated sequence in a panel of rodent-human somatic cell hybrids, and to the interval 11q12-q13.2 in a chromosome 11 somatic cell hybrid mapping panel. Confirmation of regional localisation to 11q13 has been obtained by fluorescent in situ hybridisation of genomic cosmid clones, by demonstration of the presence of both SEA (the human homologue of avian retrovirus proviral tyrosine kinase, 11q13) and CFL1 in some of these clones and by close linkage of CFL1 to SEA in a panel of high-dose irradiation hybrids. We have identified human muscle-type cofilin sequences by comparison of human expressed sequence tags with M-type cofilins of other species and we have mapped the human M-type cofilin, CFL2, to chromosome 14.
Gillett
G T
GT
MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London.
Fox
M F
MF
Rowe
P S
PS
Casimir
C M
CM
Povey
S
S
D051339:Actin Depolymerizing Factors; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D003001:Cloning, Molecular; D051340:Cofilin 2; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D051339:Actin Depolymerizing Factors; C495815:CFL2 protein, human; D051340:Cofilin 2; D017931:DNA Primers; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins