title: Functional characteristics of enhanced Fc receptor expression of beta 2 integrin-deficient bovine mononuclear phagocytes.
abstract: Fc receptor expression, cytoplasmic Ca2+ signaling, chemiluminescent (CL) response, and electron spin resonance (ESR) combined with spin trapping of blood mononuclear phagocytes from control heifers and a heifer with leukocyte adhesion deficiency (LAD) were evaluated to elucidate the relationships between complement receptor type 3 (CR3) and Fc receptor expression and their functional responses. The mean fluorescence intensity of fluorescein isothiocyanate (FITC)-conjugated anti-bovine IgG bound to mononuclear phagocytes from the heifer with LAD was 1.8-fold higher than that of control heifers. The mean increments of cytoplasmic Ca2+ concentrations of mononuclear phagocytes from the heifer with LAD stimulated with OPZ, Agg-IgG, and PMA were 39.4 (P < 0.05), 118, and 71.6% compared with those of control heifers. A 1.27-fold increase in the CL response relative to control heifers was detected when mononuclear phagocytes from the heifer with LAD were stimulated with Agg-IgG. The OPZ-induced CL response of mononuclear phagocytes from the heifer with LAD was significantly (P < 0.05) decreased, whereas the PMA-induced CL response was similar to that of control heifers. The ESR spectrum of mononuclear phagocytes from the heifer with LAD was increased when stimulated with Agg-IgG, and was impaired when stimulated by OPZ compared with that of control heifers. The ESR spectrum of mononuclear phagocytes stimulated with PMA was similar in control heifers and the heifer with LAD. Fc receptors on mononuclear phagocytes from the heifer with LAD were enhanced, and their cytoplasmic Ca2+ signaling, CL response, and ESR-spin trapping when stimulated with Agg-IgG and OPZ appeared to be associated with enhanced Fc receptors.
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
authors.forename: H
authors.initials: H
authors.lastname: Higuchi
authors.forename: N
authors.initials: N
authors.lastname: Goji
authors.forename: H
authors.initials: H
authors.lastname: Noda
authors.forename: M
authors.initials: M
authors.lastname: Kuwabara
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy / Q000662:veterinary; D005260:Female; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000276:immunology* / Q000378:metabolism / Q000662:veterinary*; D008163:Luminescent Measurements; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism*; D011961:Receptors, Fc / Q000096:biosynthesis* / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology
chemical_list: D018821:CD18 Antigens; D011961:Receptors, Fc; D002118:Calcium