title: Low-energy helium-neon laser irradiation stimulates interleukin-1 alpha and interleukin-8 release from cultured human keratinocytes.
abstract: Clinical observations have suggested that low-energy lasers might promote wound healing. Evidence suggests that He-Ne laser irradiation induces an increase in the rate of keratinocyte migration and proliferation as compared with nonirradiated controls in vitro. This study sought to determine whether He-Ne laser could induce cytokine production in cultured keratinocytes. The results revealed (i) a significant increase in interleukin-1 alpha and interleukin-8 production and their respective mRNA expression in He-Ne laser-treated groups as compared with nonirradiated controls, and (ii) under 1.5 joules/cm2 irradiation, this stimulating effect of He-Ne laser treatment is concentration-dependent. Because interleukin-1 alpha induces keratinocyte migration, this finding may partially explain the stimulatory effects on the motility of keratinocytes. As both interleukin-1 alpha and interleukin-8 provoke proliferation of keratinocytes, it is not unreasonable to propose that these two cytokines play a profound role in the enhancement of keratinocyte proliferation as a result of He-Ne laser irradiation. Our findings provide further evidence of enhanced wound healing at the cellular and molecular level as a result of the He-Ne laser.
authors.affiliation: Department of Dermatology, Kaohsiung Medical College, Taiwan, Republic of China.
authors.forename: H S
authors.initials: HS
authors.lastname: Yu
authors.forename: K L
authors.initials: KL
authors.lastname: Chang
authors.forename: C L
authors.initials: CL
authors.lastname: Yu
authors.forename: J W
authors.initials: JW
authors.lastname: Chen
authors.forename: G S
authors.initials: GS
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D006371:Helium; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects*; D007834:Lasers; D009356:Neon; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D007375:Interleukin-1; D016209:Interleukin-8; D006371:Helium; D009356:Neon