Functional expression of the human MDR1 gene in Escherichia coli.
In this preliminary study, we report the cloning of the human MDR1 cDNA into a prokaryotic expression vector and the consequent functional expression of heterologous P-glycoprotein in Escherichia coli. We demonstrate increased resistance to the P-glycoprotein substrates TPA+, TPP+, and puromycin; reduced accumulation of TPP+ and tetracycline by resistant cells; and the expression of a full-length immunoreactive P-glycoprotein molecule in the membrane fraction of resistant cells. The obvious structural and functional similarities of P-gp to prokaryotic ABC transporters and other efflux transporters argues for a more complete study of the consequences pertaining to the expression of human P-glycoprotein in E. coli.
George
A M
AM
Department of Cell and Molecular Biology, University of Technology Sydney, New South Wales, Australia.
Davey
M W
MW
Mir
A A
AA
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001152:Arsenicals / Q000493:pharmacokinetics; D001483:Base Sequence; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018432:Drug Resistance, Multiple; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008566:Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009861:Onium Compounds / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D010957:Plasmids / Q000235:genetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001152:Arsenicals; D018076:DNA, Complementary; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C009439:tetraphenylarsonium; C013289:tetraphenylphosphonium