title: Multimers of the precursor of a type IV pilin-like component of the general secretory pathway are unrelated to pili.
abstract: Both the mature and precursor forms of PulG, a type IV pilin-like component of the general secretory pathway of Klebsiella oxytoca, can be chemically cross-linked into multimers similar to those obtained by cross-linking the components of type IV pili. To explore the possibility that the PulG precursor could form a pilus-like structure, the PulG sequence was altered in a variety of ways, including (i) replacement of the characteristic hydrophobic region, which is required for the assembly of type IV pilins by the MalE signal peptide, or (ii) fusion of beta-lactamase (beta laM) to the C-terminus. Neither of these changes affected multimerization. PulG precursor could be post-translationally processed by prepilin peptidase (PulO), indicating that the N-terminus of prePulG remains on the cytoplasmic side of the cytoplasmic membrane where it is accessible to the catalytic site of this enzyme. Finally, precursor and mature forms of PulG could be efficiently cross-linked in a mixed dimer, indicating that at least a subpopulation of the two forms of the protein are probably located in clusters in the cytoplasmic membrane. These results provide further evidence that the cross-linked multimers of the precursor form of PulG are unrelated to type IV pilus-like structures. It is still unclear whether a subpopulation of processed PulG can be assembled into a rudimentary pilus-like structure.
authors.affiliation: Unité de Génétique Moléculaire (CNRS URA1149), Institut Pasteur, Paris, France. max@pasteur.fr
authors.forename: A P
authors.initials: AP
authors.lastname: Pugsley
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D036781:Fimbriae Proteins; D007709:Klebsiella / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011498:Protein Precursors; C083615:pulG protein, Klebsiella pneumoniae; D036781:Fimbriae Proteins