title: Structural and functional properties of full-length and truncated human proapolipoprotein AI expressed in escherichia coli.
abstract: Utilizing the Escherichia coli/pGex vector expression system incorporating a thrombin cleavage site, full-length (residues -6-243) and truncated forms of proapolipoprotein AI (proapoAI), terminating at amino acid residues 222, 210, 150, and 135, were purified to levels of at least 5 mg/L, after thrombin cleavage. Assessed by circular dichroism, the helical contents of L-alpha-dimyristoylphosphatidylcholine-associated forms of human plasma-derived apolipoprotein AI (apoAI) and recombinant proapoAI were comparable, being 69% and 65%, respectively. Circular dichroism measurements of the lipid-associated complexes of the truncated forms showed that between the sequence of residues 150-222 no additional helicity was gained until the carboxyl-terminal sequence was present in the molecule, indicating that the carboxyl terminus of the protein is required for the formation of helix within this central region. While tryptophan residues were more than 86% accessible, as assessed by iodide quenching, in the two truncated forms, proapoAI-6-135 and proapoAI-6-150, for both free and complexed protein, this figure fell to about 50% for full-length recombinant proapoAI, further indicating the influence of the carboxyl terminus on the structure of the whole protein. While cross-linking human plasma apoAI in solution with dithiobis-(succinimidyl propionate) revealed high molecular weight oligomers by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, recombinant proapoAI did not strongly form complexes larger than trimers. None of the truncated proapoAI molecules formed oligomers larger than trimers. The shortest form, proapoAI-6-135, only dimerized. Initial results from lecithin:cholesterol acyltransferase activation (apoAI peptide concentration 0.2 microM) indicated that truncation of the 21 carboxy-terminal amino acids resulted in a drop of approximately 53% in activation and 33 residues a drop of 67% relative to the full-length protein. Overall these results indicate the important influence of the carboxyl terminus on the structure of apoAI.
authors.affiliation: Baker Medical Research Institute, Prahran, Australia.
authors.forename: L E
authors.initials: LE
authors.lastname: Pyle
authors.forename: W H
authors.initials: WH
authors.lastname: Sawyer
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: A
authors.initials: A
authors.lastname: Mitchell
authors.forename: N H
authors.initials: NH
authors.lastname: Fidge
mesh_terms: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005454:Fluorescence Polarization; D006801:Humans; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D013917:Thrombin
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D003432:Cross-Linking Reagents; D008075:Lipoproteins, HDL; D011498:Protein Precursors; D011994:Recombinant Proteins; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D013917:Thrombin