Molecular cloning of two cannabinoid type 1-like receptor genes from the puffer fish Fugu rubripes.
The puffer fish, Fugu rubripes (Fugu), has been proposed as a model vertebrate genome. We have characterized two putative G-protein-coupled receptor encoding genes, FCB1A and FCB1B, obtained by degenerate PCR and low-stringency hybridization of a Fugu genomic library. These two genes show high homology to the human cannabinoid receptor type 1 (HCB1), but very low homology to the type 2 receptor. The amino acid sequences of the FCB1A and FCB1B genes are 66.2% identical, and the homology of each gene to HCB1 is 72.2 and 59.0%, respectively. The transcripts of both the FCB1A and the FCB1B receptors are abundant in the brain. No type 2 receptor could be cloned from Fugu. These data suggest that although two cannabinoid receptor-like genes are found in the puffer fish, both show similarity to the type 1 receptor found in human.
Yamaguchi
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Department of Medicine, Addenbrookes Hospital, Cambridge, United Kingdom.
Macrae
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AD
Brenner
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002186:Cannabinoids; D003001:Cloning, Molecular; D004247:DNA; D005400:Fishes, Poisonous / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D002186:Cannabinoids; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D004247:DNA