No difference in the proportion of insulin receptor exon 11 +/- isoform mRNA in the liver of rats after development of hypertension.
1. There are two functionally different isoforms of the insulin receptor in humans and rats. We hypothesized that a change in their relative proportion could be of relevance to insulin resistance in hypertension. 2. A reverse-transcriptase polymerase chain reaction technique was established for the detection of mRNA for the exon 11+ and exon 11- isoforms and the proportion of each was determined in 3, 6, 9 and 12 week old spontaneously hypertensive rats and Wistar-Kyoto rats, as well as adrenocorticotrophin (ACTH)-induced hypertensive rats and controls. 3. The proportion of the exon 11+ form (approximately 95%) and exon 11- form (approximately 5%) was similar in the liver of all rats studied. 4. We conclude that there is no change in insulin receptor isoform expression in the liver in the models of hypertension studied.
Lou
Y
Y
Department of Physiology, University of Sydney, New South Wales, Australia.
Zee
R Y
RY
Li
M
M
Morris
B J
BJ
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D005091:Exons / Q000502:physiology*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007536:Isomerism; D008099:Liver / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011972:Receptor, Insulin / Q000235:genetics*
D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone; D011972:Receptor, Insulin