Complex interaction between different proteinaceous components within the cell-wall structure of Candida albicans.
We have previously described a monoclonal antibody, MAb DC3:H10, which recognized an epitope preferentially expressed on the surface of Candida albicans germ tubes. In the present study we examined the MAb-reactive material further. Immunoblot analysis of the material purified partially by Sephadex G-200 and DEAE-Sephacel chromatography reacted with antibodies to the C. albicans C3d receptor (CR2). In an ELISA, MAb DC3:H10 captured antigen that was recognized by both anti-CR2 and anti-mp58 fibrinogen binding mannoprotein polyclonal antibodies. The MAb DC3:H10 failed to compete with either of these antisera in an ELISA. Indirect immunofluorescence (IIF) analysis showed differences in surface distribution for the MAb DC3:H10, the CR2, and the mp 58 epitopes. Dual labeling IIF experiments showed MAb DC3:H10 binding to be unaffected by binding of fibrinogen or anti-mp58 antibody. However, the binding patterns of MAb DC3:H10 were modified in the presence of anti-CR2 antibody, suggesting a complex interaction between these cell wall components.
Lopez-Ribot
J L
JL
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
Cortlandt
D A
DA
Straus
D C
DC
Morrow
K J
KJ
Chaffin
W L
WL
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000737:chemistry* / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D015151:Immunoblotting; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D017464:Receptors, Complement 3d / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D017464:Receptors, Complement 3d; C025363:mannoproteins