title: A monoclonal antibody specific for carboxy-terminal region of yeast translation elongation factor-3 inhibits ribosome-activated ATPase activity but not intrinsic ATPase activity.
abstract: Elongation factor-3 (EF-3) is an essential translation elongation factor specific to yeasts and fungi. When EF-3 interacts with yeast ribosomes, its ATPase activity, which is indispensable for the function of EF-3 in translation, is drastically enhanced. In this study, a monoclonal antibody specific for the carboxy-terminal region inhibited the ribosome-activated ATPase activity of EF-3, while it did not inhibit the intrinsic ATPase activity of EF-3. The results suggest that the carboxy-terminal region of EF-3 is involved in the interaction with yeast ribosomes. The monoclonal antibody also inhibited poly(U)-directed poly(Phe) synthesis, which indicates that the carboxy-terminal region is important for EF-3 to express its function in the polypeptide elongation cycle.
authors.affiliation: Department of Chemistry, Faculty of Science, Shizuoka University, Japan.
authors.forename: U
authors.initials: U
authors.lastname: Masahiro
authors.forename: T
authors.initials: T
authors.lastname: Atsushi
authors.forename: N
authors.initials: N
authors.lastname: Kazutoshi
authors.forename: I
authors.initials: I
authors.lastname: Miho
authors.forename: I
authors.initials: I
authors.lastname: Makoto
authors.forename: A
authors.initials: A
authors.lastname: Mikio
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects*; D018604:Epitope Mapping; D005656:Fungal Proteins; D010445:Peptide Elongation Factors / Q000276:immunology*; D012270:Ribosomes / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae; D000251:Adenosine Triphosphatases