The effects of mesoporphyrin on experimental arthritis in mice.
The effects of mesoporphyrin, a novel porphyrin derivative, on type II collagen-induced arthritis in mice were studied. Mesoporphyrin (10-30 mg/kg) and prednisolone (5 mg/kg; reference drug) reduced the incidence and severity of type II collagen-induced arthritis in mice, as assayed by clinical observation and histopathological studies. Although both agents inhibited type II collagen-induced delayed type hypersensitivity in arthritic mice, only prednisolone inhibited humoral immunity to type II collagen. The effects of mesoporphyrin on T cell dependent allergic inflammation were examined, in order to study the mechanism by which it inhibits arthritis. Staphylococcal enterotoxin B (SEB; superantigen)-potentiated collagen-induced arthritis and sheep red blood cell-induced delayed type hypersensitivity reaction were clearly inhibited by mesoporphyrin. Moreover, the superantigen-induced CD-25 expression on T cells was inhibited by mesoporphyrin. These results indicate that mesoporphyrin inhibits type II collagen-induced arthritis by inhibiting the activation of T cells.
Nagai
H
H
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Takaoka
Y
Y
Mori
H
H
Matsuura
N
N
D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000188:drug therapy*; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics; D003094:Collagen / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000008:administration & dosage / Q000633:toxicity; D006968:Hypersensitivity, Delayed; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008652:Mesoporphyrins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D008811:Mice, Inbred DBA; D011239:Prednisolone / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D012015:Reference Standards; D013210:Staphylococcus / Q000378:metabolism; D018089:Superantigens / Q000633:toxicity; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D015704:CD4 Antigens; D004768:Enterotoxins; D008652:Mesoporphyrins; D015375:Receptors, Interleukin-2; D018089:Superantigens; C031468:mesoporphyrin IX; D003094:Collagen; D011239:Prednisolone