Cloning and characterization of a cDNA encoding a Rab1-like small GTP-binding protein from Petunia hybrida.
The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to RB11_HUMAN and PhPCRGP3 to RAB1A_HUMAN. Using PhPCRGP3 as a probe, 8 identical clones were selected from a Petunia leaf cDNA library. They all encode the same 22.5 kDa polypeptide, PhRAB1, able to bind GTP in vitro and 72% identical to RAB1A_HUMAN. Hybridizable mRNAs encoding PhRAB1 accumulated preferentially in opened flowers.
Jako
C
C
ENSAM-INRA, CNRS URA 573, Laboratoire de Biochimie et Physiologie Végétales, Montpellier, France.
Teyssendier de la Serve
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D020691:rab GTP-Binding Proteins; D020761:rab1 GTP-Binding Proteins
D018076:DNA, Complementary; D018744:DNA, Plant; C101510:PCRGP1 protein, Petunia hybrida; C101511:PCRGP2 protein, Petunia hybrida; C101512:PCRGP3 protein, Petunia hybrida; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins; D020761:rab1 GTP-Binding Proteins