Carbohydrate epitopes on Haemonchus contortus antigens.
Extracts of infective larvae and adults of the trichostrongylid Haemonchus contortus were studied for the presence of carbohydrate moieties. Several different lectin-binding sites were demonstrated in both stages using a panel of nine lectins. The carbohydrate specificity of the lectins used strongly suggests that alpha-D-mannose, alpha-D-glucose, and D-N-acetylglucosamine are the most important carbohydrate epitopes present on H. contortus proteins. Thus, N-linked oligosaccharides form the major part of the carbohydrate moieties on these glycoproteins. Treatment with sodium periodate was performed to investigate the immunoreactivity towards the carbohydrate moieties. This treatment resulted in a reduction in the immunoreactivity of these antigens as demonstrated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting, suggesting that a substantial part of the host immune response against H. contortus is directed against the carbohydrate epitopes on the parasite antigens.
Schallig
H D
HD
Faculty of Veterinary Medicine, Utrecht University, Netherlands.
van Leeuwen
M A
MA
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006189:Haemonchus / Q000276:immunology*; D015151:Immunoblotting; D037102:Lectins / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology*
D000947:Antigens, Helminth; D002241:Carbohydrates; D000939:Epitopes; D037102:Lectins; D010504:Periodic Acid; C009288:metaperiodate