Cyclooxygenase induction is essential for NGF synthesis enhancement by NGF inducers in L-M cells.
Nerve growth factor (NGF) inducers, for example, 4-methylcatechol, pyrroloquinoline quinone, kansuinin A, and ingenol triacetate, stimulate NGF synthesis in L-M cells, but the mechanism of NGF induction by NGF inducers is not known. Using the four different types of previously described NGF inducers, we proved induction of cyclooxygenase activity by NGF inducers and detected prostaglandins D2 and E2 as metabolites of arachidonic acid. From the observation that the induction of NGF by each NGF inducers was inhibited by cyclooxygenase inhibitors or dexamethasone, cyclooxygenase activation is supposed to be an essential process for NGF induction.
Yamaguchi
K
K
Sagami Chemical Research Center, Kanagawa, Japan.
Tsuji
T
T
Uemura
D
D
Kondo
K
K
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D003067:Coenzymes / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D007509:Irritants / Q000494:pharmacology; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D045542:PQQ Cofactor; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000975:Antioxidants; D002396:Catechols; D003067:Coenzymes; D004224:Diterpenes; D007509:Irritants; D009414:Nerve Growth Factors; D015363:Quinolones; C018599:4-methylcatechol; D016718:Arachidonic Acid; C104302:kansuinin A; D045542:PQQ Cofactor; D011451:Prostaglandin-Endoperoxide Synthases; C008125:ingenol