Insect neuropeptide F (NPF)-related peptides: isolation from Colorado potato beetle (Leptinotarsa decemlineata) brain.
Two novel neuropeptides with neuropeptide F (NPF)-like immunoreactivity have been isolated from brain extracts of the Colorado potato beetle. Purification was achieved primarily by use of reverse phase chromatography including initial C-18 Sep-Pak cartridges and 4 subsequent analytical HPLC columns. Combined data from automated Edman degradation, immunochemical analysis, u.v. absorbance and mass spectrometry led to the elucidation of their full primary structures. The deduced sequences are: Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-NH2 (ARGPQLRLRFamide) and Ala-Pro-Ser-Leu-Arg-Leu-Arg-Phe-NH2 (APSLRLRFamide). On the basis of their primary structure both peptides can be appended to the invertebrate group of neuropeptide Y (NPY)-like peptides, generally referred to as NPFs. We suggest these peptides to be designated Led-NPF-1 and Led-NPF-2.
Spittaels
K
K
Zoological Institute of the University, Leuven, Belgium.
Verhaert
P
P
Shaw
C
C
Johnston
R N
RN
Devreese
B
B
Van Beeumen
J
J
De Loof
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D001517:Coleoptera / Q000737:chemistry*; D017952:Ganglia, Invertebrate / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*; D011863:Radioimmunoassay; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009479:Neuropeptides; C076101:neuropeptide F