title: Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2.
abstract: We have cloned and expressed in Escherichia coli cDNA for rat nuclear transport factor 2 (NTF2), a key cytoplasmic factor that facilitates the import of proteins into the nucleus through nuclear pores. We have used this bacterially expressed material to produce orthorhombic crystals suitable for high-resolution X-ray diffraction structure determination. The crystals have P212121 symmetry with a = 55.9 A; b = 56.7 A; c = 88.3 A and diffract past 2 A on laboratory X-ray sources. The asymmetric unit of these crystals contains two NTF2 polypeptide chains, consistent with the reported dimeric structure of the molecule.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, England.
authors.forename: H M
authors.initials: HM
authors.lastname: Kent
authors.forename: W D
authors.initials: WD
authors.lastname: Clarkson
authors.forename: T L
authors.initials: TL
authors.lastname: Bullock
authors.forename: M
authors.initials: M
authors.lastname: Stewart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D029543:Nucleocytoplasmic Transport Proteins; D011487:Protein Conformation; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; C503612:Nutf2 protein, rat; D011993:Recombinant Fusion Proteins