Study of the biotransformation of a potential benzo[c]fluorene antineoplastic using high-performance liquid chromatography with high-speed-scanning ultraviolet detection.
As the sum of benfluron metabolites found was only a part of the total amount applied, a search for undiscovered metabolites was undertaken in the extracts from isolated rat hepatocytes and in the bile and perfusate in the experiments with an isolated perfused rat liver. To identify the metabolites, high-performance liquid chromatography with UV spectral analysis was used, as benfluron derivatives exhibit characteristic absorption spectra. Administration of known metabolites to experimental animals and selective induction of certain metabolic pathways led to the finding of new metabolites and of the respective conjugates. Fast atom bombardment-mass spectrometry analysis was used to identify the newly found metabolites and conjugates.
Nobilis
M
M
Institute of Experimental Biopharmaceutics, Joint Research Center of Academy of the Sciences of the Czech Republic, Hradec Králové, Czech Republic.
Anzenbacher
P
P
Pastera
J
J
Svoboda
Z
Z
Hrubý
K
K
Kvĕtina
J
J
Ubik
K
K
Trejtnar
F
F
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D001646:Bile / Q000737:chemistry; D001711:Biotransformation; D002273:Carcinogens / Q000737:chemistry*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D002273:Carcinogens; D005449:Fluorenes; C041513:benzo(c)fluorene; C034234:VUFB 13468