Uptake, reflux, and excretion of bromosulfophthalein in ischaemia-reperfusion injury of rat liver.
The uptake, reflux and excretion of bromosulfophthalein (BSP) were studied on a model of total warm ischaemia for 30 min (group 1) or 60 min (group 2) followed by reperfusion for 45 min in the isolated perfused rat liver of unfasting rats. In group 1, the BSP hepatic uptake was comparable to control livers (30 s ischaemia plus 45 min reperfusion), but was significantly reduced in group 2. The reflux of BSP from liver to perfusate in group 1 and group 2 resulted in the appearance of secondary concentration time peaks of BSP in the reservoir perfusate. This result suggests that ischaemia-reperfusion induced a qualitative change in BSP pharmacokinetics. Excretion of the dye into bile was significantly impaired in group 2 only. The leakage of lactate dehydrogenase into the perfusate was increased moderately in both group 1 and group 2 in comparison to the controls, suggesting a low degree of liver parenchymal injury. In conclusion, the results of this investigation showed that BSP pharmacokinetics were not only undergoing quantitative changes but also a qualitative change in the model of ischaemia-reperfusion injury of the liver obtained from fed rats and may thus serve as a highly sensitive indicator of liver viability.
Kukan
M
M
Institute of Preventive and Clinical Medicine, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Bezek
S
S
Trnovec
T
T
D000818:Animals; D019540:Area Under Curve; D001646:Bile / Q000378:metabolism / Q000502:physiology; D004396:Coloring Agents / Q000493:pharmacokinetics*; D007511:Ischemia / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008102:Liver Circulation / Q000502:physiology*; D008297:Male; D009929:Organ Size / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D013448:Sulfobromophthalein / Q000493:pharmacokinetics*
D004396:Coloring Agents; D013448:Sulfobromophthalein; D007770:L-Lactate Dehydrogenase