Immunomodulatory effect of recombinant human superoxide dismutase (SOD) on human B lymphocyte function in vitro.
Recent investigations demonstrate that B lymphocytes possess an oxygen-generating system which is similar to the phagocytic NADPH-oxidase system. Reduction of nitroblue tetrazolium by stimulated tonsillar B cells is inhibited by superoxide dismutase (SOD). However, the biological significance of the superoxide-generating property of B cells remains to be explored. In this study, we examined the immunomodulatory effect of a recombinant human SOD (rh-SOD) on the activation of human B lymphocytes in vitro. A supplement of rh-SOD in the B cell culture increased the proliferation of unstimulated B cells in the presence of SAC, but not of SAC-preactivated B cells in the presence of cytokines such as IL-2 or IL-4. In addition, rh-SOD enhanced the immunoglobulin generation by B cells at the terminal stage of differentiation. Inactivation of the enzymatic activity of SOD by treatment with anti-SOD antibody abrogated the enhancing effects. These data suggest that the superoxide-generating system in B cells may be involved in the cellular activation process.
Morikawa
K
K
Department of Internal Medicine, Shimane Medical University, Japan.
Morikawa
S
S
D000906:Antibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger; D011994:Recombinant Proteins / Q000276:immunology; D013482:Superoxide Dismutase / Q000276:immunology / Q000494:pharmacology*; D013997:Time Factors
D000906:Antibodies; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013482:Superoxide Dismutase