title: A serine-kinase associated with the p127-l(2)gl tumour suppressor of Drosophila may regulate the binding of p127 to nonmuscle myosin II heavy chain and the attachment of p127 to the plasma membrane.
abstract: The p127 tumour suppressor protein encoded by the lethal(2)giant larvae, [l(2)gl], gene of Drosophila melanogaster is a component of a cytoskeletal network distributed in both the cytoplasm and on the inner face of the plasma membrane. The p127 protein forms high molecular mass complexes consisting mainly of homo-oligomerized p127 molecules and at least ten additional proteins. One of these proteins has been recently identified as nonmuscle myosin type II heavy chain. To determine the functional interactions between p127 and other proteins present in the p127 complexes, we analyzed p127 for posttranslational modifications and found that p127 can be phosphorylated at serine residues. In this report we describe the characteristics of a serine kinase which is associated with p127, as judged by its recovery in p127 complexes purified by either gel filtration or immuno-affinity chromatography. This kinase phosphorylates p127 in vitro and its activation by supplementing ATP results in the release of p127 from the plasma membrane. Moreover, similar activation of the kinase present in immuno-purified p127 complexes dissociates nonmuscle myosin II from p127 without affecting the homo-oligomerization of p127. This dissociation can be inhibited by staurosporine and a 26mer peptide covering amino acid positions 651 to 676 of p127 and containing five serine residues which are evolutionarily conserved from Drosophila to humans. These results indicate that a serine-kinase tightly associated with p127 regulates p127 binding with components of the cytoskeleton present in both the cytoplasm and on the plasma membrane.
authors.affiliation: Department of Developmental Genetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kalmes
authors.forename: G
authors.initials: G
authors.lastname: Merdes
authors.forename: B
authors.initials: B
authors.lastname: Neumann
authors.forename: D
authors.initials: D
authors.lastname: Strand
authors.forename: B M
authors.initials: BM
authors.lastname: Mechler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D017124:Conserved Sequence; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D004789:Enzyme Activation; D017344:Genes, Insect; D016147:Genes, Tumor Suppressor; D006801:Humans; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018995:Myosin Heavy Chains / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; D046911:Macromolecular Substances; D025521:Tumor Suppressor Proteins; C081435:l(2)gl protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D018995:Myosin Heavy Chains