Oxytocin is hydrolyzed by an enzyme in human placenta that is identical to the oxytocinase of pregnancy serum.
The hydrolysis of oxytocin (OT) by human placental subcellular fractions and pregnant sera was studied in the presence of bestatin, a potent inhibitor of aminopeptidases, and the antibody against pregnant serum oxyotocinase (P-LAP)(EC 3.4 11.3) by measuring liberated amino acids by high performance liquid chromatography (HPLC). Our immunotitration study and the effect of bastatin on the oxytocin-degrading protease showed that the initiating and responsible protease in oxyotocin degradation in human placenta and pregnant serum is P-LAP.
Naruki
M
M
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
Mizutani
S
S
Goto
K
K
Tsujimoto
M
M
Nakazato
H
H
Itakura
A
A
Mizuno
K
K
Kurauchi
O
O
Kikkawa
F
F
Tomoda
Y
Y
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D010122:Cystinyl Aminopeptidase / Q000097:blood*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D010121:Oxytocin; D004492:Edetic Acid; D007931:Leucyl Aminopeptidase; D010122:Cystinyl Aminopeptidase; D007930:Leucine; C012211:ubenimex