Trypsin-catalyzed peptide synthesis and various p-guanidinophenyl esters as acyl donors.
Trypsin-catalyzed peptide synthesis has been studied by using p-guanidinophenyl esters of N alpha-(tert-butyloxycarbonyl)amino acid and peptide as acyl donor components. The reaction conditions were optimized for organic solvents, pH, and concentration of acceptor. The method was especially useful for the preparation of various peptides containing D-amino acids. The enzymatic hydrolysis of the resulting products was negligible.
Sekizaki
H
H
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Itoh
K
K
Toyota
E
E
Tanizawa
K
K
D000215:Acylation; D000595:Amino Acid Sequence; D004952:Esters / Q000737:chemistry*; D006146:Guanidines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D004952:Esters; D006146:Guanidines; D007202:Indicators and Reagents; D010455:Peptides; D014357:Trypsin