Marked increases in neuronal nitric oxide synthase (nNOS) mRNA and NADPH-diaphorase histostaining in adrenal cortex after immobilization stress in rats.
The changes of neuronal nitric oxide synthase (nNOS) mRNA expression in the rat adrenal gland following immobilization-induced stress were examined by the in situ hybridization technique. To introduce immobilization stress, the animals were wrapped with flexible wire gauze for 6 h. In the adrenal medulla and cortex, signals for nNOS mRNA were detected. In the adrenal medulla, the difference between non-stressed and stressed animals was not clear. In the adrenal cortex, the expression of nNOS mRNA markedly increased (2.5-fold) in the stressed animals. Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-diaphorase) histochemistry also showed an increase of staining in adrenal cortex after stress. This suggests that nitric oxide (NO) is involved in stress-induced activation in adrenal cortex function.
Tsuchiya
T
T
Life Science Research Laboratories, Shiseido Research Center, Yokohama, Japan.
Kishimoto
J
J
Nakayama
Y
Y
D000302:Adrenal Cortex / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D006651:Histocytochemistry; D007103:Immobilization; D017403:In Situ Hybridization; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000201:enzymology / Q000378:metabolism*
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase