[Natural process of wound healing of photocoagulated retinal pigment epithelium in culture--observation of DNA synthesis by BrDu incorporation].
We examined the proliferation of retinal pigment epithelial (RPE) cells after krypton laser photocoagulation in culture. A pigmented monolayer of chick embryonic RPE cells was cultured on a collagen membrane placed on collagen gel. RPE cells were labeled with bromodeoxyuridine (BrDu) every 12 hours until 7 1/2 days after the photocoagulation and stained immunocytochemically with anti BrDu antibody. Immediately after the photocoagulation, RPE cells became detached at the burned lesion and the collagen membrane beneath the RPE layer was exposed. Some cells adjacent to the burned lesion showed DNA synthesis and subsequent mitosis between 12 to 24 hours after the photocoagulation. Cells with labeled nuclei migrated into the denuded burned area after 24 hours and covered the whole burned area within three days after the photocoagulation. DNA synthesis continued in these on the burned lesion after complete coverage of the lesion but stopped temporarily 3 1/2 to 4 1/2 days after the photocoagulation. Thereafter DNA synthesis increased again and continued until the end of the experiment. Such use of the cultured RPE cells might be useful in studying cellular reaction after photocoagulation.
Karasawa
Y
Y
Department of Ophthalmology, National Defense Medical College, Saitama-ken, Japan.
Mizukawa
A
A
Okisaka
S
S
D000818:Animals; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis*; D008028:Light Coagulation; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
D004247:DNA; D001973:Bromodeoxyuridine