Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection.
In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue.
Vitale
A A
AA
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires, Argentina.
Ferrari
C C
CC
Aldana
H
H
Affanni
J M
JM
D000085:Acetates; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D008550:Melatonin / Q000032:analysis*; D008832:Microchemistry; D010870:Pineal Gland / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000085:Acetates; C007650:ethyl acetate; D008550:Melatonin