title: Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines.
abstract: The yeast transcriptional adaptor, Gcn5p, is a catalytic subunit of a nuclear (type A) histone acetyltransferase linking histone acetylation to gene activation. Here we report that Gcn5p acetylates histones H3 and H4 non-randomly at specific lysines in the amino-terminal domains. Lysine 14 of H3 and lysines 8 and 16 of H4 are highly preferred acetylation sites for Gcn5p. We also demonstrate that lysine 9 is the preferred position of acetylation in newly synthesized yeast H3 in vivo. This finding, along with the fact that lysines 5 and 12 in H4 are predominant acetylation sites during chromatin assembly of many organisms, indicates that Gcn5p acetylates a distinct set of lysines that do not overlap with those sites characteristically used by type B histone acetyltransferases for histone deposition and chromatin assembly.
authors.affiliation: Department of Biology, University of Rochester, New York 14627, USA.
authors.forename: M H
authors.initials: MH
authors.lastname: Kuo
authors.forename: J E
authors.initials: JE
authors.lastname: Brownell
authors.forename: R E
authors.initials: RE
authors.lastname: Sobel
authors.forename: T A
authors.initials: TA
authors.lastname: Ranalli
authors.forename: R G
authors.initials: RG
authors.lastname: Cook
authors.forename: D G
authors.initials: DG
authors.lastname: Edmondson
authors.forename: S Y
authors.initials: SY
authors.lastname: Roth
authors.forename: C D
authors.initials: CD
authors.lastname: Allis
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D016808:Tetrahymena thermophila / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D011494:Protein Kinases; D008239:Lysine