title: High purity factor IX and prothrombin complex concentrate (PCC): pharmacokinetics and evidence that factor IXa is the thrombogenic trigger in PCC.
abstract: Recent studies using assays for surrogate markers of thrombogenicity in man have demonstrated that activation of the coagulation system occurs following infusion of clinical doses of prothrombin complex concentrates (PCC) but not after the same doses of high-purity factor IX concentrates (HP-FIX) in patients with haemophilia B. Here we have investigated the mechanism of such thrombogenesis by applying assays that detect early-through to late-events in coagulation system activation in a pharmacokinetic cross-over study of 50 IU/kg PCC and a new HP-FIX product in haemophilia B patients. Satisfactory recoveries and half-lives were observed for both concentrates. HP-FIX caused no increases in thrombin-antithrombin III complex (TAT), prothrombin activation peptide fragment F1+2 (F1+2), factor X activation peptide (FXAP) or factor VIIa (FVIIa). In contrast the same dose of factor IX in the form of PCC was followed by significant increases over pre-infusion levels of TAT, F1+2 and FXAP, but not FVIIa. Elevations of FIXAP occurred after both HP-FIX and PCC but did not reach normal levels and were attributed to normalisation of the FIX concentration in those patients whose levels of FIXAP were initially low. We conclude that the thrombogenic trigger associated with PCC infusion occurs at the level of factor X activation. In the absence of any increase in FVIIa, we would attribute this to the likely presence of FIXa in the PCC.
authors.affiliation: Dept. of Haematology, Charing Cross and Westminster Medical School, London, United Kingdom.
authors.forename: H
authors.initials: H
authors.lastname: Philippou
authors.forename: A
authors.initials: A
authors.lastname: Adami
authors.forename: D A
authors.initials: DA
authors.lastname: Lane
authors.forename: I R
authors.initials: IR
authors.lastname: MacGregor
authors.forename: E G
authors.initials: EG
authors.lastname: Tuddenham
authors.forename: G D
authors.initials: GD
authors.lastname: Lowe
authors.forename: A
authors.initials: A
authors.lastname: Rumley
authors.forename: C A
authors.initials: CA
authors.lastname: Ludlam
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects*; D001779:Blood Coagulation Factors / Q000493:pharmacokinetics* / Q000627:therapeutic use; D015949:Factor IXa / Q000493:pharmacokinetics* / Q000627:therapeutic use; D002836:Hemophilia B / Q000097:blood* / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D001779:Blood Coagulation Factors; C025667:prothrombin complex concentrates; D015949:Factor IXa