Secretion of GDNF by glial cells does not account for the neurotrophic effect of bFGF on dopamine neurons in vitro.
The neurotrophic effect of bFGF on dopamine (DA) neurons in vitro depends upon its mitogenic activity on glial cells. We examined whether glial secretion of the dopaminergic growth factor GDNF is responsible for the trophic activity of bFGF on mesencephalic DA neurons. When added with lower concentrations of bFGF, GDNF had an additive trophic effect, which disappeared at optimal bFGF concentrations. However, addition of GDNF-neutralizing antibodies could not modify the bFGF-induced survival and growth of DA neurons. These results indicate that although GDNF could be secreted by bFGF stimulated glia, its secretion alone does not account for the trophic effect of bFGF on mesencephalic DA neurons.
Hou
J G
JG
Department of Neurology, Mount Sinai School of Medicine, New York, N.Y. 10029, USA.
Mytilineou
C
C
D000818:Animals; D000918:Antibody Specificity; D001692:Biological Transport / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism*; D009500:Neutralization Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D014316:Tritium; D016222:Fibroblast Growth Factor 2; D004298:Dopamine