Alteration of acylphosphatase levels in familial Alzheimer's disease fibroblasts with presenilin gene mutations.
Acylphosphatase (AcPase), an enzyme that modulates the activity of Ca(2+)-ATPase by hydrolysing its phosphorylated moiety, has been found to be significantly higher in cultured skin fibroblasts from donors affected by early onset familial Alzheimer's disease (EOFAD) with PS-1 and PS-2 gene mutations. Of the two known isoenzymes of acylphosphatase, only the erythrocyte one accounts for the total increase in activity. No relevant alteration was observed in phosphotyrosine phosphatase activity (PTPase), in Ca(2+)-ATPase and Na+, K(+)-ATPase activities of the same cells as compared to age-matched controls. This finding could suggest a possible explanation for the calcium-dependent biochemical alterations previously described in Alzheimer's disease fibroblasts.
Liguri
G
G
Department of Biochemical Sciences, University of Florence, Italy.
Cecchi
C
C
Latorraca
S
S
Pieri
A
A
Sorbi
S
S
Degl'Innocenti
D
D
Ramponi
G
G
D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation / Q000502:physiology*; D053764:Presenilin-1; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D012867:Skin / Q000166:cytology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D000252:Calcium-Transporting ATPases; D007527:Isoenzymes; D008565:Membrane Proteins; D053764:Presenilin-1; D017027:Protein Tyrosine Phosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D000097024:Acylphosphatase; C508853:PSEN1 protein, human