Agarose gel analysis of 15-40-kb PCR amplimers.
Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.
Burland
V
V
FMC BioProducts, Rockland, ME, USA. valerie_burland@fmc.com
Curtis
F P
FP
Kusukawa
N
N
D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D008274:Magnesium / Q000008:administration & dosage; D010781:Photography; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling; D013698:Templates, Genetic
D004279:DNA, Viral; D008274:Magnesium