Transforming growth factor-beta 1 promotes re-elongation of injured axons of cultured rat hippocampal neurons.
Transforming growth factor-beta 1 (TGF-beta 1) is highly expressed in neural tissues following brain injuries. To determine a role of TGF-beta 1 in neural pathologies, axons of cultured rat hippocampal neurons were cut by local irradiation of laser beam, and the effect of TGF-beta 1 on neurite regeneration following axotomy was investigated. The axonal growth was stopped by laser irradiation, but addition of TGF-beta 1 remarkably promoted the axonal re-elongation from the injured site. The effect of TGF-beta 1 was concentration dependent and seen maximally at a concentration of 1 ng/ml. These results suggest that TGF-beta 1 has a capability of promoting axonal regeneration of brain neurons after lesioning.
Abe
K
K
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113, Japan.
Chu
P J
PJ
Ishihara
A
A
Saito
H
H
D000818:Animals; D001369:Axons / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta