title: Inhaled albuterol does not inhibit cellular influx or lung injury produced by segmental antigen challenge in humans.
abstract: Many experimental protocols and published guidelines for performing bronchoscopy, bronchoalveolar lavage (BAL), bronchial biopsies, and segmental antigen challenge (SAC) of allergic asthmatic subjects recommend treating subjects with a beta-agonist prior to the procedure. However, the effect of beta-agonist pretreatment has not been reported. In a retrospective analysis of ragweed allergic subjects undergoing bronchoscopy, SAC, and BAL, we examined the effect of albuterol pretreatment on cellular influx and lung injury produced by antigen challenge. Forty-eight subjects, 17 who received no pretreatment and 31 who received four puffs of albuterol prior to bronchoscopy, comprised the study groups. No parameter monitored in BAL fluid 24 h after SAC (total cells, macrophages, neutrophils, eosinophils, lymphocytes, total protein, albumin, or eosinophil cationic protein) differed in subjects pretreated with albuterol when compared with subjects who were not pretreated. Although additional, prospective studies are warranted, we conclude that beta-agonist pretreatment of experimental subjects does not alter many aspects of the inflammatory response produced by SAC.
authors.affiliation: Department of Medicine, Jefferson Medical College, Thomas Jefferson University Philadelphia, Pennsylvania 19107-5083, USA.
authors.forename: J
authors.initials: J
authors.lastname: O'Connor
authors.forename: G C
authors.initials: GC
authors.lastname: Kane
authors.forename: M
authors.initials: M
authors.lastname: Tolino
authors.forename: M
authors.initials: M
authors.lastname: Pollice
authors.forename: J E
authors.initials: JE
authors.lastname: Fish
authors.forename: S P
authors.initials: SP
authors.lastname: Peters
mesh_terms: D000280:Administration, Inhalation; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000328:Adult; D000420:Albuterol / Q000494:pharmacology*; D000941:Antigens / Q000008:administration & dosage*; D001249:Asthma / Q000276:immunology*; D001985:Bronchial Provocation Tests; D018893:Bronchoalveolar Lavage; D004804:Eosinophils / Q000187:drug effects; D005260:Female; D006801:Humans; D008168:Lung / Q000276:immunology* / Q000502:physiology; D008297:Male; D011058:Pollen / Q000276:immunology*; D012189:Retrospective Studies; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012882:Skin Tests
chemical_list: D000318:Adrenergic beta-Agonists; D000941:Antigens; D000420:Albuterol