Detection of the plasma cholinesterase K variant by PCR using an amplification-created restriction site.
Ten individuals registered at the Danish Cholinesterase Research Unit were examined at the DNA level for the presence of the K allele of plasma cholinesterase, using amplification-created restriction sites (ACRSs). A further nine members of a family registered at the unit were tested for mutations of the K and atypical variants. The frequency of the K allele was calculated from examination of normal material from 25 individuals, representing 50 random alleles. The results show that the ACRS method successfully demonstrates the presence of the K variant, whose frequency in the Danish population was found to be 0.18. We conclude that this technique is a reliable and rapid non-radioactive diagnostic assay for detecting the plasma cholinesterase K variant.
Jensen
F S
FS
Department of Anaesthesia, National University Hospital, Copenhagen, Denmark.
Nielsen
L R
LR
Schwartz
M
M
D000483:Alleles; D001483:Base Sequence; D002802:Cholinesterases / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D017931:DNA Primers; D002802:Cholinesterases