Gene expression of NOR-1, a neuron-derived orphan receptor, is inducible in neuronal and other cell lineages in culture.
We recently isolated a cDNA of NOR-1, a novel member of the steroid/thyroid hormone receptor superfamily, from a rat neuronal cell cDNA library. NOR-1 has strong amino acid sequence homology to orphan receptors NGFI-B and Nurr1, and is expressed predominantly in the brain tissue in vivo. In the present study, we examined NOR-1 gene expression in various cultured cells with distinct properties of neuronal cells (PC12), glial cells (C6), endocrine cells (GH3, AtT-20 and Y-1), or fibroblasts (NIH3T3 and L), by reverse transcription-polymerase chain reaction and nucleotide sequencing. NOR-1 transcripts were induced with forskolin and 12-O-tetradecanoylphorbol-13-acetate in all cell lines examined. The nerve growth factor induced not only NGFI-B but also NOR-1 and Nurr1 transcripts in PC12 cells. These findings suggest that NOR-1 gene expression is induced by various stimuli in diverse cell types.
Bandoh
S
S
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Tsukada
T
T
Maruyama
K
K
Ohkura
N
N
Yamaguchi
K
K
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D007739:L Cells; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology; D016716:PC12 Cells; D051381:Rats; D011987:Receptors, Steroid / Q000235:genetics*; D011988:Receptors, Thyroid Hormone; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C490411:Nr4a3 protein, mouse; C091333:Nr4a3 protein, rat; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D005576:Colforsin