Continuous Cell Line from Pupal Ovary of Perina nuda (Lepidoptera: Lymantriidae) That Is Permissive to Nuclear Polyhedrosis Virus from P. nuda
A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28&deg;C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).
Wang
CH
Department of Plant Pathology and Entomology, National Taiwan University, Taipei, Taiwan, ROC
Chou
CM
Liu
HC
Kau
SL
Kou
GH
Lo
CF