Molecular epidemiology of Escherichia coli O157:H7 by pulsed-field gel electrophoresis and comparison with that by bacteriophage typing.
One hundred twenty-four Escherichia coli O157:H7 isolates were characterized by pulse-field gel electrophoresis, bacteriophage typing, and PCR of verotoxin genes. Diversity indices obtained--0.786 for phage types and 0.987 for pulsed-field gel electrophoresis types--demonstrated that phage typing falls below the critical value (0.9) required for confident interpretation of results.
Krause
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Department of Medical Microbiology, Medical School, Foresterhill, Aberdeen, United Kingdom.
Thomson-Carter
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Pennington
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D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D002417:Cattle; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1; D013552:Swine
D001427:Bacterial Toxins; D022622:Shiga Toxin 1