5-HT1A receptor expression is modulated by corticosteroid receptor agonists in primary rat hippocampal culture.
The effects of corticosteroid receptor agonists on the expression of 5-HT1A receptor mRNA were measured in rat hippocampal cultures using in situ hybridization histochemistry. In our normal culture system, grown in serum and steroid-free media, moderate to heavy signal for 5-HT1A mRNA transcripts were detected in hippocampal neurons and glial cells. Aldosterone, a type I corticosteroid receptor agonist (10(-9) M), significantly reduced the expression of 5-HT1A mRNA both in neurons and glial fibrillary acidic protein (GFAP)-immunoreactive (IR) cells. The type II corticosteroid receptor agonist, Ru28362 (10(-8) M), also significantly decreased neuronal 5-HT1A mRNA expression. However, it was not as effective as aldosterone in reducing the label over GFAP-IR cells. These data indicate that corticosteroids may directly regulate the expression of hippocampal 5-HT1A receptors at the mRNA level in cultured hippocampal cells.
Nishi
M
M
Department of Biology, New York University, NY 10003, USA.
Azmitia
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EC
D000450:Aldosterone / Q000494:pharmacology; D000732:Androstanols / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D006651:Histocytochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats / Q000196:embryology; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism*; D011987:Receptors, Steroid / Q000819:agonists*; D012702:Serotonin Antagonists / Q000494:pharmacology
D000732:Androstanols; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011987:Receptors, Steroid; D012702:Serotonin Antagonists; D000450:Aldosterone; C049154:11,17-dihydroxy-6-methyl-17-(1-propynyl)androsta-1,4,6-triene-3-one