PCR-SSCP analysis of the promoter region of the renin gene in patients with aldosterone-producing adenomas.
1. Aldosterone-producing adenomas (APA) of the adrenal gland may be responsive or un-responsive to the renin-angiotensin system. 2. We have described increased expression of renin mRNA in angiotensin-responsive aldosterone-producing adenomas (AII-R-APA) compared with angiotensin-un-responsive aldosterone-producing adenomas (AII-U-APA) and significantly different allelic frequencies of the BglI, TaqI and HinfI restriction fragment length polymorphisms of the renin gene between the two groups. 3. An area including the 5' flanking region -500 bp from exon 1, exon 1 and intron A contained no gross insertions or deletions when studied by a long polymerase chain reaction technique. 4. In the present study, polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) revealed no single base pair alteration in the proximal promoter region (-600 bp to transcription start) of the renin gene in patients with APA (either AII-U-APA or AII-R-APA) when compared with normal subjects. 5. Therefore, mutations in this regulatory region do not appear to explain the different levels of renin gene expression observed in these two subtypes of APA.
Ballantine
D M
DM
Hypertension Unit, Greenslopes Hospital, Brisbane, Queensland, Australia.
Klemm
S A
SA
Tunny
T J
TJ
Stowasser
M
M
Gordon
R D
RD
D000236:Adenoma / Q000235:genetics* / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000450:Aldosterone / Q000096:biosynthesis*; D015342:DNA Probes; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012083:Renin / Q000235:genetics*
D015342:DNA Probes; D000450:Aldosterone; D012083:Renin