Intracellular compartmentalization of two differentially spliced s-rex/NSP mRNAs in neurons.
Using a subtractive hybridization technique directed to cloning transcripts with compartmentalized distributions within cerebral cortex neurons, we have isolated rat s-rex mRNAs that are analogues of the human neuroendocrine-specific NSP gene transcripts. Differential splicing produces two main s-rex mRNA that have different regional distributions in the developing and mature rat nervous system. In certain populations of adult brain neurons, most of s-rexs, mRNA and a substantial amount of s-rexb mRNA are localized to the axonal pole of the cell body. The localization of S-Rex/NSP proteins in these neurons suggests that s-rex mRNA compartmentalization targets the encoded proteins to specific regions of the neuron.
Baka
I D
ID
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Ninkina
N N
NN
Pinon
L G
LG
Adu
J
J
Davies
A M
AM
Georgiev
G P
GP
Buchman
V L
VL
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000737:chemistry; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002451:Cell Compartmentation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013574:Synaptosomes / Q000737:chemistry
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C081781:RTN1 protein, human; C489914:Rtn1 protein, mouse; C489913:Rtn1 protein, rat