title: Oxytocin is hydrolyzed by an enzyme in human placenta that is identical to the oxytocinase of pregnancy serum.
abstract: The hydrolysis of oxytocin (OT) by human placental subcellular fractions and pregnant sera was studied in the presence of bestatin, a potent inhibitor of aminopeptidases, and the antibody against pregnant serum oxyotocinase (P-LAP)(EC 3.4 11.3) by measuring liberated amino acids by high performance liquid chromatography (HPLC). Our immunotitration study and the effect of bastatin on the oxytocin-degrading protease showed that the initiating and responsible protease in oxyotocin degradation in human placenta and pregnant serum is P-LAP.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Naruki
authors.forename: S
authors.initials: S
authors.lastname: Mizutani
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: H
authors.initials: H
authors.lastname: Nakazato
authors.forename: A
authors.initials: A
authors.lastname: Itakura
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: O
authors.initials: O
authors.lastname: Kurauchi
authors.forename: F
authors.initials: F
authors.lastname: Kikkawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D010122:Cystinyl Aminopeptidase / Q000097:blood*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
chemical_list: D010121:Oxytocin; D004492:Edetic Acid; D007931:Leucyl Aminopeptidase; D010122:Cystinyl Aminopeptidase; D007930:Leucine; C012211:ubenimex