Prenatal alcohol exposure and the effects of environmental enrichment on hippocampal dendritic spine density.
The effects of environmental enrichment on synaptic spine density in hippocampal area CAI were examined in rats exposed prenatally to alcohol. Pregnant dams were given ethanol via intragastric intubation (6 g/kg/day) from gestational days 8 through 19, or given isocaloric sucrose. An untreated control group was also used. After weaning, offspring from the three groups were then reared for 10 weeks in either isolated (caged alone, not handled) or enriched (group housed with "toys," handled) conditions. Animals were then sacrificed, the brains Golgi impregnated, and CAI pyramidal cell apical and basilar spine densities quantified. Among isolated animals there were no significant differences between control and alcohol-exposed groups. In general, environmental enrichment increased apical or basilar spine densities in untreated and sucrose controls. However, in prenatal alcohol-exposed animals, environmental enrichment did not increase spine densities. Because the environmental enrichment acted postnatally, these findings suggest that the effects of prenatal alcohol exposure included decreased neural plasticity enduring into early adulthood. Such a reduction in neuroanatomical plasticity in hippocampus may be associated with cognitive impairments found following prenatal alcohol exposure.
Berman
R F
RF
Fetal Alcohol Research Center, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
Hannigan
J H
JH
Sperry
M A
MA
Zajac
C S
CS
D000818:Animals; D002492:Central Nervous System Depressants / Q000633:toxicity*; D004396:Coloring Agents; D003712:Dendrites / Q000187:drug effects / Q000648:ultrastructure*; D004777:Environment; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017966:Pyramidal Cells / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D012727:Sex Characteristics; D013569:Synapses / Q000187:drug effects / Q000648:ultrastructure; D015430:Weight Gain / Q000187:drug effects
D002492:Central Nervous System Depressants; D004396:Coloring Agents; D000431:Ethanol