A comparative study of HPLC and TLC separation of amino acids using Cu(II) ion.
Separation of amino acids using Cu(II) by RP-HPLC and impregnated TLC is reported. For HPLC the sample mixture of amino acids containing copper (II) was injected into the column while for TLC the silica gel plates were impregnated with Cu(II). The mobile phase used in HPLC was acetate buffer (0.3 M, pH 6.0)-acetonitrile (9:1, v/v), and that in TLC was acetate buffer (0.3 M, pH 6.0):acetonitrile-n-butanol (12:5:10, by vol.). The results have been compared and discussed.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Ali
I
I
Sharma
S
S
D000596:Amino Acids / Q000302:isolation & purification*; D002413:Cations, Divalent; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003300:Copper; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D058428:Silica Gel; D012822:Silicon Dioxide
D000596:Amino Acids; D002413:Cations, Divalent; D007202:Indicators and Reagents; D058428:Silica Gel; D012822:Silicon Dioxide; D003300:Copper