Colorimetric susceptibility testing for Aspergillus fumigatus: comparison of menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and Alamar blue tests.
Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans was determined. However, only the MTT test generated interpretable data when Aspergillus fumigatus was used.
Jahn
B
B
Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University, Mainz, Germany. bjahn@mzdmza.zdv.uni-mainz.de
Stüben
A
A
Bhakdi
S
S
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010078:Oxazines; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*; D014966:Xanthenes
D000935:Antifungal Agents; D004396:Coloring Agents; D010078:Oxazines; D013778:Tetrazolium Salts; D013844:Thiazoles; D014966:Xanthenes; C005843:resazurin; D000666:Amphotericin B; C022616:thiazolyl blue