title: Utilization of the same DNA replication origin by human cells of different derivation.
abstract: In the past, a highly sensitive and efficient method was developed to map DNA replication origins in human cells, based on quantitative PCR performed on nascent DNA samples. This method allowed the identification of a replication origin in the myeloid HL-60 cell line, located on chromosome 19 within an approximately 500 bp segment near the lamin B2 gene [Giacca et al. (1994) Proc. Natl. Acad. Sci. USA, 91, 7119]. The same procedure has now been further simplified and extended to a variety of other exponentially growing human cells of different histological derivation (three neural, one connectival and one epithelial), with a nearly diploid chromosomal content. In all the six cell lines tested, the origin activity within the lamin B2 gene domain was localized to the same region. Furthermore, the lamin B2 origin was also found to be active in stimulated, but not in quiescent, peripheral blood lymphocytes.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Giacca
authors.forename: P
authors.initials: P
authors.lastname: Norio
authors.forename: G
authors.initials: G
authors.lastname: Biamonti
authors.forename: S
authors.initials: S
authors.lastname: Riva
authors.forename: A
authors.initials: A
authors.lastname: Falaschi
mesh_terms: D001854:Bone Marrow Cells; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D004261:DNA Replication; D004847:Epithelial Cells; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D034921:Lamin Type B; D034882:Lamins; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000166:cytology; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018741:Replication Origin
chemical_list: D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins