title: KatG sequence deletion is not the major cause of isoniazid resistance in Japanese and Yemeni Mycobacterium tuberculosis isolates.
abstract: One of the mechanisms of isoniazid resistance to Mycobacterium tuberculosis has been proved to be the chromosomal deletion of katG. Based on this finding, 22 isoniazid-resistant isolates of M. tuberculosis obtained in Japan and Yemen were analysed for katG by polymerase chain reaction and catalase activity. Only six (27%) of the 22 isolates were compatible with the mechanism (lack of amplification of katG and loss of catalase activity). In contrast, eight isolates (36%) were katG positive but catalase activity-negative and eight (36%) were positive for both factors, indicating that isoniazid resistance is multifactorial and the deletion of katG was not the major cause of resistance in the isolates examined in this study.
authors.affiliation: Department of Infectious Diseases, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: N
authors.initials: N
authors.lastname: Tachikawa
authors.forename: K
authors.initials: K
authors.lastname: Kitada
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: C
authors.initials: C
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: S
authors.initials: S
authors.lastname: Kimura
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D007564:Japan; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D009519:New York City; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D014376:Tuberculosis / Q000382:microbiology; D015006:Yemen
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010544:Peroxidases; C076136:catalase HPI; D007538:Isoniazid