title: Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro.
abstract: The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Chiba-ken, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizushina
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Kurosawa
authors.forename: M
authors.initials: M
authors.lastname: Onoue
authors.forename: H
authors.initials: H
authors.lastname: Seto
authors.forename: T
authors.initials: T
authors.lastname: Horie
authors.forename: N
authors.initials: N
authors.lastname: Aoyagi
authors.forename: M
authors.initials: M
authors.lastname: Yamaoka
authors.forename: A
authors.initials: A
authors.lastname: Matsukage
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D002417:Cattle; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013942:Thymine Nucleotides / Q000378:metabolism
chemical_list: D003902:Detergents; D005227:Fatty Acids; D008041:Linoleic Acids; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D019787:Linoleic Acid; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate