title: Plant virus DNA replication processes in Agrobacterium: insight into the origins of geminiviruses?
abstract: Agrobacterium tumefaciens, a bacterial plant pathogen, when transformed with plasmid constructs containing greater than unit length DNA of tomato leaf curl geminivirus accumulates viral replicative form DNAs indistinguishable from those produced in infected plants. The accumulation of the viral DNA species depends on the presence of two origins of replication in the DNA constructs and is drastically reduced by introducing mutations into the viral replication-associated protein (Rep or C1) ORF, indicating that an active viral replication process is occurring in the bacterial cell. The accumulation of these viral DNA species is not affected by mutations or deletions in the other viral open reading frames. The observation that geminivirus DNA replication functions are supported by the bacterial cellular machinery provides evidence for the theory that these circular single-stranded DNA viruses have evolved from prokaryotic episomal replicons.
authors.affiliation: Division of Horticulture, Commonwealth Scientific and Industrial Research Organization, Adelaide, Australia.
authors.forename: J E
authors.initials: JE
authors.lastname: Rigden
authors.forename: I B
authors.initials: IB
authors.lastname: Dry
authors.forename: L R
authors.initials: LR
authors.lastname: Krake
authors.forename: M A
authors.initials: MA
authors.lastname: Rezaian
mesh_terms: D005075:Biological Evolution; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D017791:Geminiviridae / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D008957:Models, Genetic; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000821:virology
chemical_list: D004279:DNA, Viral