title: A One-Bead One-Peptide Combinatorial Library Method for B-Cell Epitope Mapping
abstract: The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide-beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-beta-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) anti-human insulin (discontinuous epitope), and (iv) surface immunoglobulins (&mu;kappa) of two murine B-cell lymphoma cell lines (antigen unknown).
authors.affiliation: Arizona Cancer Center and Department of Medicine, University of Arizona College of Medicine, Tucson, Arizona, 85724
authors.initials: KS
authors.lastname: Lam
authors.initials: D
authors.lastname: Lake
authors.initials: SE
authors.lastname: Salmon
authors.initials: J
authors.lastname: Smith
authors.initials: ML
authors.lastname: Chen
authors.initials: S
authors.lastname: Wade
authors.initials: F
authors.lastname: Abdul-Latif
authors.initials: RJ
authors.lastname: Knapp
authors.initials: Z
authors.lastname: Leblova
authors.initials: RD
authors.lastname: Ferguson
authors.initials: V
authors.lastname: Krchnak
authors.initials: NF
authors.lastname: Sepetov
authors.initials: M
authors.lastname: Lebl