title: Preparation, purification and morphology of polymeric nanoparticles as drug carriers.
abstract: The aim of this work was to prepare biodegradable poly(D,L-lactic acid-co-glycolic acid) copolymer (PLAGA) nanoparticles by the solvent evaporation process and to incorporate an antifungal antibiotic, amphotericin B. Blank nanoparticles obtained were 130 +/- 27 nm in diameter. When amphotericin B was added in the organic phase, the final suspension showed two populations due to unbound drug. Free amphotericin B was removed by contacting the nanoparticle suspension with an adsorbent polymer. Amberlite XAD16, and subsequently ultrafiltering the medium. The drug payload was between 0.7 and 1.3%. To gain more insight in the cause of this low loading, we studied progesterone-loaded nanoparticles using PLAGA and polystyrene as models because progesterone and these polymers exhibit a degree of miscibility. In the case of polystyrene, nanoparticle drug content reached 8%.
authors.affiliation: Laboratoire de Pharmacie Galénique, Faculté de Pharmacie, Angers, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Venier-Julienne
authors.forename: J P
authors.initials: JP
authors.lastname: Benoît
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage; D004337:Drug Carriers; D019344:Lactic Acid; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D011374:Progesterone / Q000008:administration & dosage
chemical_list: D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011374:Progesterone; D000666:Amphotericin B