A comparative study of the polymerase chain reaction and local antibody production in acute retinal necrosis syndrome and cytomegalovirus retinitis.
BACKGROUND
It has been thought that herpes viruses may play an important role in acute retinal necrosis syndrome (herpes simplex and varicella-zoster viruses) as well as in cytomegalovirus retinitis and it would be useful to know the specificity of the methods for detecting the viruses.


METHODS
Amplification of the herpetic viral genome DNA by polymerase chain reaction (PCR) in aqueous and vitreous humor was compared with Goldmann-Witmer coefficients against herpetic antigens in five patients with acute retinal necrosis syndrome and in two patients with cytomegalovirus retinitis, using vitreous samples to determine the specificity of these diagnostic methods.


RESULTS
The varicella-zoster virus genome DNA was amplified by PCR in four of the five patients with acute retinal necrosis syndrome, and cytomegalovirus genome DNA was enhanced in both patients with cytomegalovirus retinitis. Four patients who exhibited the varicella-zoster viral genome showed marked increase of the Goldmann-Witmer coefficient against varicella-zoster virus. Conversely, the two patients with cytomegalovirus retinitis showed no remarkable changes among the antigens.


CONCLUSIONS
Our results showed that the amplification of the viral genome DNA in the samples by PCR is specific in both diseases, and that the increased level of local antibody production also is specific in varicella-zoster retinitis. In cytomegalovirus retinitis, however, antibody production against cytomegalovirus does not show increase of the Goldmann-Witmer coefficient.
Abe
T
T
Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan.
Tsuchida
K
K
Tamai
M
M
D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001082:Aqueous Humor / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology; D017726:Cytomegalovirus Retinitis / Q000175:diagnosis*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D015828:Eye Infections, Viral / Q000175:diagnosis; D005260:Female; D005451:Fluorescein Angiography; D005654:Fundus Oculi; D006561:Herpes Simplex / Q000175:diagnosis*; D006562:Herpes Zoster / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015882:Retinal Necrosis Syndrome, Acute / Q000175:diagnosis* / Q000821:virology; D014822:Vitreous Body / Q000821:virology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral