title: Identification of the polyamine N8-acetyltransferase involved in the pathway of 1,3-diaminopropane production in Acanthamoeba culbertsoni.
abstract: A cytosolic polyamine N-acetyltransferase that preferentially catalyzes the acetylation of spermidine in the N8-position was identified in the free-living pathogenic amoeba Acanthamoeba culbertsoni. In addition to spermidine, the enzyme also catalyzed the acetylation of spermine and putrescine with Michaelis constants (Km values) of 97, 12, and 10 microM, respectively. The Km value for acetylcoenzyme A (acetyl-CoA) was estimated to be 11 microM, whereas CoA had an inhibitory constant of 6 microM. The N-acetylase has a molecular mass of approximately 45 kDa. That the enzyme preferentially catalyzed the acetylation of spermidine at the N8-position, resulting in N8-acetylspermidine, the preferred substrate of the polyamine oxidase found in A. culbertsoni, indicates a role for the enzyme in the production of 1,3-diaminopropane, the major polyamine found in the Acanthamoeba.
authors.affiliation: Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Shukla
authors.forename: S O
authors.initials: SO
authors.lastname: Aisien
authors.forename: B
authors.initials: B
authors.lastname: Bergmann
authors.forename: C
authors.initials: C
authors.lastname: Hellmund
authors.forename: R D
authors.initials: RD
authors.lastname: Walter
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism*; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D003959:Diamines / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism*; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D003959:Diamines; D011073:Polyamines; D015800:Protozoan Proteins; D000099228:Diamine N-Acetyltransferase; C009475:trimethylenediamine