Identification of an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) minimal promoter regulated by light and the circadian clock.
Transcription of the Arabidopsis thaliana gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is organ-specific, light-responsive, and regulated by the circadian clock. RCA is transcribed throughout the green parts of the plant, but not in roots and petals. Responses elicited by short pulses of light indicate that the light response is mediated, at least in part, by phytochrome. Analysis of transgenic tobacco and Arabidopsis carrying RCA 5' untranscribed regions fused to reporter genes (uidA, encoding beta-glucuronidase, or cat, encoding chloramphenicol acetyltransferase) indicate that elements sufficient to confer organ-specific, light-responsive, and clock-regulated transcription are localized within 317 base pairs upstream of the site of transcription initiation. A clock-responsive element sufficient to confer a low-amplitude (approximately 2-fold) circadian oscillation lies within 317 base pairs of the trascription start, but other elements necessary for high-amplitude (approximately 10-fold) circadian oscillation lie upstream of -317 and are removed by deletion from -970 to -317.
Liu
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Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA.
Taub
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McClung
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D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002940:Circadian Rhythm; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis; D008027:Light; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis*; D014026:Nicotiana; D014158:Transcription, Genetic
D017931:DNA Primers; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C054765:rca protein, plant; D015500:Chloramphenicol O-Acetyltransferase; D005966:Glucuronidase; D012273:Ribulose-Bisphosphate Carboxylase