Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy.
Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21+, CD3-) and three T-cell lymphomas (all CD3+, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T-and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.
Caniatti
M
M
Istituto di Anatomia Patologica Veterinaria e Patologia Aviare, Università di Milano, Italy.
Roccabianca
P
P
Scanziani
E
E
Paltrinieri
S
S
Moore
P F
PF
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D001707:Biopsy, Needle / Q000662:veterinary; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping / Q000662:veterinary; D008223:Lymphoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D011817:Rabbits
D015703:Antigens, CD