title: Expression of a developmentally regulated gene, Mng10, in identified neurosecretory cells in the CNS of Manduca sexta.
abstract: We are interested in the molecular events underlying the development of the nervous system of Manduca sexta during the final 24 h of the pupal molt. In this article we describe a gene, Mng10, that is expressed in the abdominal nervous system of M. sexta and is developmentally regulated over this 24-h period. In situ hybridization analysis shows that the transcript is localized predominantly to a single pair of uniquely identifiable neurosecretory neurons, the NS-L1 cells in the abdominal ganglia. Mng10 is a single copy gene encoding a 229 amino acid protein with a predicted molecular mass of 26 kDa. At the amino acid level the protein shows 34% identity to the yeast transcription unit, Yer082. Northern blot analysis shows that the transcript of Mng10 is very rare, comprising about 0.001% of the poly (A)+ RNA from the CNS and is detectable at 4 h but not 24 h prior to pupal ecdysis. One of the physiological events that develops over the final 24 h of the pupal molt is the ability of the nervous system to respond to the neuropeptide eclosion hormone. In this context, it is interesting to note that the NS-L1 cells are members of the group of 50 neurons that show increased cGMP immunoreactivity when the nervous system is exposed to the neuropeptide eclosion hormone.
authors.affiliation: Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson 85721, USA.
authors.forename: M
authors.initials: M
authors.lastname: Mészáros
authors.forename: D B
authors.initials: DB
authors.lastname: Morton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002490:Central Nervous System / Q000502:physiology*; D018076:DNA, Complementary / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017344:Genes, Insect / Q000235:genetics*; D016678:Genome; D017403:In Situ Hybridization; D018402:Manduca / Q000235:genetics*; D008969:Molecular Sequence Data; D009490:Neurosecretory Systems / Q000166:cytology / Q000502:physiology*; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense