Direct secretory effect of interleukin-1 via type I receptors in human colonic mucous epithelial cells (HT29-C1.16E).
The stable differentiated human colonic epithelial cell line, HT29-C1.16E, was used to study the effects of interleukin-1 (IL-1) on mucin exocytosis. The main findings include: (a) IL-1 stimulated a rapid release of mucin from filter grown HT29-C1.16E cells, this effect being dose related; (b) this secretory effect was abolished in the presence of the blocking monoclonal antibody M4 specific for IL-1 receptors type I, showing that IL-1 receptors type I mediated IL-1 action; (c) experiments based on chamber cultures showed that these receptors were located on the basolateral membranes of HT29-C1.16E cells; (d) finally, mRNA for IL-1 receptors type I were detected by reverse transcriptase-polymerase chain reaction in these cells. To extend these findings to the in vivo situation, the rapid stimulatory effect of IL-1 on mucin exocytosis may contribute to the wash out of noxious agents during mucosal inflammation.
Jarry
A
A
Groupe de Recherche 'Fonctions Sécrétoires des Epithéliums Digestifs', CJF INSERM 94-04, Nantes, France.
Vallette
G
G
Branka
J E
JE
Laboisse
C
C
D000911:Antibodies, Monoclonal / Q000187:drug effects; D002460:Cell Line; D003106:Colon / Q000737:chemistry* / Q000166:cytology; D005089:Exocytosis / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D009077:Mucins / Q000187:drug effects* / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D009077:Mucins; D017472:Receptors, Interleukin-1