Down-regulation of Na channel expression by A23187 in N1E-115 neuroblastoma cells.
Growth of cultured N1E-115 neuroblastoma cells in 1 microM A23187 for 2 days to elevate internal Ca reduced both membrane Na current and the transient, but not steady state, component of outward K current. Na channel mRNA abundance was reduced by an average value of 45% without effect on Kv3.1. Increases in internal Ca may therefore control excitability by independent regulation of Na and K channel mRNA abundance in neurons.
Hirsh
J K
JK
Department of Molecular Biophysics and Physiology, Rush University, Chicago, IL 60612, USA.
Quandt
F N
FN
D001483:Base Sequence; D000001:Calcimycin / Q000494:pharmacology*; D015536:Down-Regulation; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D015222:Sodium Channels / Q000187:drug effects* / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D015222:Sodium Channels; D000001:Calcimycin