title: Entamoeba histolytica: proteinase secretion induced by collagen type I is dependent on cytoskeleton integrity.
abstract: Proteolytic activities of the protozoan parasite Entamoeba histolytica strain HM1:IMSS and the cytochalasin D-resistant mutant BG-3 were analyzed following stimulation with collagen type I and Ca2+, which induces electron-dense associated collagenase secretion. The mutant BG-3 had a protease activity of 73 kDa and secretion of total protease activity was not stimulated by collagen type I and Ca2+, which produced, in contrast, a 2-fold increase in protease secretion by the parental strain. This collagen-stimulated protease secretion was inhibited by cytochalasin D at a concentration of 1 microgram/ml. Cytochalasin D did not have any effect on the protease activity released by the mutant BG-3. These findings suggest that cytoskeleton integrity is necessary for collagen-induced protease secretion.
authors.affiliation: Department of Cell Biology, Centro de Investigacion y de Estudios Avanzados del I.P.N., México DF, Mexico.
authors.forename: J J
authors.initials: JJ
authors.lastname: Serrano
authors.forename: M
authors.initials: M
authors.lastname: de la Garza
authors.forename: M
authors.initials: M
authors.lastname: Reyes
authors.forename: G
authors.initials: G
authors.lastname: León
authors.forename: R
authors.initials: R
authors.lastname: Tovar
authors.forename: M K
authors.initials: MK
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D010450:Endopeptidases / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D018093:Gelatinases / Q000378:metabolism; D009154:Mutation; D015800:Protozoan Proteins / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D015638:Cytochalasin D; D003094:Collagen; D010450:Endopeptidases; D018093:Gelatinases; D002118:Calcium