[The natural killer activity of human peripheral blood studied by a radioimmune method and by using flow cytofluorometry].
Using flow cytometry and radioimmunological test, a study was made of cytotoxic activities of natural killer (NK) cells of human peripheral blood of three patient groups: healthy donors, persons with infection pathology, and persons being in extreme situations (hyperoxygen gas mixture). With the increase in effector and target (K562) cell amount ratio, NK activity was seen elevating in all groups of patients examined. A correlation was obtained between NK activity in healthy donors and in patients examined by both the methods. NK activity in sick patients, registered by the two methods, was considerably lower than in healthy donors. NK activity in persons that appeared in extreme situations, registered by the radioimmunological test only, did not differ from that in healthy donors, whereas NK activity, registered by the flow cytometry, was found to be considerably lower, resp.
Atochina
O V
OV
Neustroev
A P
AP
D001782:Blood Donors; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018496:Hyperoxia / Q000276:immunology; D007239:Infections / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods