Defective ATP-dependent mucin secretion by cystic fibrosis pancreatic epithelial cells.
The response of confluent monolayers of normal and cystic fibrosis (CF) pancreatic epithelial cells to stimulation by extracellular ATP and ATP analogues was investigated in terms of mucin secretion. Mucin secretion was measured as release of M1 antigens by a direct sandwich enzyme immunoassay. Extracellular ATP provoked rapid (< or = 15 min) and strong mucin secretion (+ 480 +/- 35%) by the normal pancreatic cell lines but was not able to induce mucin secretion by the CF cell lines. The order of efficacy of nucleotide agonists with ATP > ADP > AMP > adenosine was that of typical P2-purinergic receptors. ATP induced a rapid and transient intracellular [Ca2+] mobilization in both normal and CF pancreatic epithelial cells. This work demonstrated that CFTR seemed to mediate ATP-dependent mucin secretion.
Montserrat
C
C
Groupe de Recherche sur les Glandes Exocrines, Faculté de Médecine, Marseille, France.
Merten
M
M
Figarella
C
C
D000230:Adenocarcinoma; D000241:Adenosine / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000328:Adult; D000941:Antigens / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000503:physiopathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis / Q000502:physiology; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007118:Immunoassay; D009077:Mucins / Q000032:analysis / Q000378:metabolism*; D010190:Pancreatic Neoplasms; D018048:Receptors, Purinergic P2 / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D000941:Antigens; C505032:CFTR protein, human; D009077:Mucins; D018048:Receptors, Purinergic P2; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002217:Carbachol; D000241:Adenosine; D002118:Calcium