Inhibitory effects of selenium, vitamin A and butylated hydroxytoluene on growth of human maxillary cancer cells in vitro.
The effects of vitamin A, selenium, and butylated hydroxytoluene (BHT) on the growth of a human maxillary cancer cell line were examined in monolayer cell cultures. The colony-forming assay showed a 50% reduction in the survival rate of the cell line at a concentration of 3.6 micrograms/ml of selenium, 28 micrograms/ml of vitamin A, and 74 micrograms/ml of BHT. Flow cytometric analysis with both FITC-labeled bromodeoxyuridine monoclonal antibody and propidium iodide demonstrated an increase of the S-phase fraction in the presence of selenium, an increase of the G0/G1-phase fraction in the presence of vitamin A, and an increase of the G2-M-phase fraction 1 day followed by an increase of G0/G1-phase fraction from the 3rd to 7th day when BHT was added. These results suggest that the mechanisms of inhibition of DNA synthesis by these compounds are different.
Yamamoto
Y
Y
Department of Otolaryngology, Osaka Medical College, Japan.
Inoue
I
I
Takasaki
T
T
Takahashi
H
H
D000911:Antibodies, Monoclonal; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D006801:Humans; D008437:Maxilla / Q000187:drug effects* / Q000473:pathology*; D008441:Maxillary Neoplasms / Q000473:pathology / Q000517:prevention & control*; D012643:Selenium / Q000494:pharmacology*; D014801:Vitamin A / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D014801:Vitamin A; D002084:Butylated Hydroxytoluene; D012643:Selenium