Vasopressin content in the cerebrospinal fluid and fluid perfusing cerebral ventricles in rats after the afferent vagus nerve fibres stimulation.
Experiments were carried out on male rats in urethane anaesthesia. Cerebroventricular system was perfused with McIlwain-Rodniht's solution from lateral ventricles to cerebellomedullary cistern. Both vagus nerves were cut and the central ends of the nerves were electrically stimulated during the collection of the third 30-min portion of perfusing fluid. Vasopressin (AVP) was determined by radioimmunoassay in samples of the cerebrospinal fluid (CSF) (the first portion) and in five successive samples of the perfusing fluid. AVP concentration in the CSF was several times greater than in the fluid perfusing cerebral ventricles. Alternate electrical stimulation of both vagus nerves did not change considerably the release of AVP into the fluid perfusing the cerebral ventricles in rat, although a certain upward tendency could be observed. It seems that only AVP raised in circulating blood and not in CSF, after vagus nerves stimulation may act on the central nervous structures.
Orłowska-Majdak
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M
Traczyk
W Z
WZ
D000818:Animals; D002552:Cerebral Ventricles / Q000378:metabolism*; D004558:Electric Stimulation; D008297:Male; D051381:Rats; D011915:Rats, Inbred BUF; D017208:Rats, Wistar; D012119:Respiration / Q000502:physiology; D014628:Vagotomy; D014630:Vagus Nerve / Q000502:physiology* / Q000601:surgery; D014667:Vasopressins / Q000134:cerebrospinal fluid*
D014667:Vasopressins