Determination of avermectin B1 in biological samples by immunoaffinity column cleanup and liquid chromatography with UV detection.
A simple and specific procedure for determination of avermectin B1 in biological samples is described. A specific anti-avermectin B1 immunosorbent was developed. Avermectin B1 was extracted from cattle plasma, cattle meat, and pear samples. The extracts were cleaned up on a solid-phase extraction column (C18) and an immunoaffinity column. Avermectin B1 was determined by reversed-phase liquid chromatography with UV detection at 245 nm. Recoveries of avermectin B1 from samples spiked at levels of 6 and 60 parts per billion (ppb) ranged from 80 to 86%, with coefficients of variation of 5-14%. The limit of detection was 2 ppb. The procedure provides a simple and sensitive method for monitoring avermectin B1 residues in biological matrixes.
Li
J
J
Beijing Agricultural University, College of Veterinary Medicine, Department of Veterinary Pharmacology and Toxicology, Peoples Republic of China.
Qian
C
C
D000818:Animals; D000871:Anthelmintics / Q000032:analysis* / Q000097:blood; D000918:Antibody Specificity; D002417:Cattle; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry*; D007164:Immunosorbents / Q000737:chemistry; D007306:Insecticides / Q000032:analysis* / Q000097:blood; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D008460:Meat / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000871:Anthelmintics; D007164:Immunosorbents; D007306:Insecticides; C048324:abamectin; D007559:Ivermectin