Molecular detection or carriers of hemophilia A in Mexican families.
The genomic DNAs of carrier mothers from 20 hemophilia A unrelated Mexican families were analysed by polymerase chain reaction (PCR) amplification of the Bcl I polymorphic region at intron 18 of the factor VIII gene. Eleven women (55%) were found to be informative (Bcl I+/Bcl I-), eight (40%) were Bcl I- homozygotes and one (5%) was a Bcl I+ homozygote. In 18 daughters of the informative families we were able to establish carrier status for eight. The frequency of the Bcl I alleles in the 20 mothers was 0.675 for Bcl I- and 0.325 for Bcl I+ which give a frequency of 44% of heterozygous females in our sample.
Rojas-Martínez
A
A
Departamento de Bioquímica, Facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, N.L., México.
Villalobos-Torres
M C
MC
Ortiz-de-Luna
R I
RI
Pompa-Garza
M T
MT
Barrera-Saldaña
H A
HA
D005260:Female; D006467:Hemophilia A / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008800:Mexico; D010375:Pedigree