Structure and expression of the tobacco nuclear gene encoding RNA-binding protein RZ-1: the existence of an intron in the 3'-untranslated region.
We have previously characterized a tobacco cDNA encoding a novel type RNA-binding protein (RZ-1), which contains a zinc finger motif in addition to a consensus sequence-type RNA-binding domain and is localized in the nucleus. Here we isolated its genomic clone from a Nicotiana sylvestris genomic library. Southern blot analysis suggested that RZ-1 is coded for by a single locus per haploid genome. Comparison of the cDNA and genomic sequences indicated that the RZ-1 gene contains two introns, one in the coding region and another in the 3'-untranslated region. RT-PCR and ribonuclease protection analyses showed that splicing of RZ-1 pre-mRNA occurs efficiently. The RZ-1 protein is actively synthesized in rapidly dividing tobacco cells, as demonstrated by immunoblot analysis.
Hanano
S
S
Center for Gene Research, Nagoya University, Japan.
Sugita
M
M
Sugiura
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics*; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C100818:RZ-1 protein, Nicotiana sylvestris