title: Phospholipid hydroperoxides and lipid peroxidation in liver and plasma of ODS rats supplemented with alpha-tocopherol and ascorbic acid.
abstract: Forty-five mutant male ODS rats, unable to synthesize ascorbic acid, were fed nine diets containing 5, 50 or 250 mg of vitamin E/kg diet and 150, 300 or 900 mg of vitamin C/kg diet for 21 days. The concentrations of vitamins C and E increased in liver and plasma in relation to the level of these vitamins in the diet. Vitamin C dietary supplementation increased the plasma vitamin E content at low levels of vitamin E intake, supporting the concept of an in vivo synergism between both antioxidant vitamins. Vitamin C, at the dietary levels studied, did not affect the lipid peroxidation. Vitamin E decreased liver and plasma endogenous levels of thiobarbituric acid-reactive substances and liver sensitivity to non-enzymatic lipid peroxidation. This was confirmed by a highly specific assay of lipid hydroperoxides using high performance liquid chromatography with chemiluminescence detection. The hepatic concentration of both phosphatidylcholine and phosphatidylethanolamine hydroperoxides decreased as the vitamin E content of the diet increased. The results show for the first time the capacity of vitamin E to protest against peroxidation of major phospholipids in vivo under basal unstressed conditions.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Cadenas
authors.forename: S
authors.initials: S
authors.lastname: Lertsiri
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
authors.forename: G
authors.initials: G
authors.lastname: Barja
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism*; D050544:L-Gulonolactone Oxidase; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000378:metabolism*; D051381:Rats; D011922:Rats, Mutant Strains; D013401:Sugar Alcohol Dehydrogenases / Q000172:deficiency; D014810:Vitamin E / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D010743:Phospholipids; D014810:Vitamin E; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid