Antigen-specific IgA-secreting cells induced by peripheral vaccination.
Antigen-specific IgA-secreting cells were shown to be generated in peripheral lymph nodes following s.c. vaccination. The efferent duct of prefemoral lymph nodes of sheep were cannulated prior to vaccination at a site draining to the cannulated node. Vaccines were contained a recombinant protein (Taenia ovis 45W-GST) and either incomplete Freund's, Quil A or Al(OH)3 as adjuvant. Lymph fluid was examined for the presence of 45W-GST-specific antibody by ELISA and antibody-secreting cells by enzyme-linked immunospot (ELISPOT) assay. Large numbers of anti-45W-GST IgA-secreting cells were detected at various times after vaccination as were IgM, IgG1 and IgG2-secreting cells. For sheep vaccinated using incomplete Freund's adjuvant, up to 11% (> 1 million/day) of 45W-GST-specific ELISPOT were IgA-secreting cells.
Rothel
J S
JS
University of Melbourne, Molecular Parasitology Laboratory, Werribee, Victoria, Australia.
Corner
L A
LA
Seow
H F
HF
Wood
P R
PR
Lightowlers
M W
MW
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D012756:Sheep; D013621:Taenia / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D000939:Epitopes; D007070:Immunoglobulin A; D014614:Vaccines, Synthetic