Role of the composite glucocorticoid response element in proliferin gene expression.
A binding site for the glucocorticoid receptor in the serum-inducible proliferin gene promoter has been reported to function as a composite glucocorticoid response element when fused to a minimal promoter. We now show that this element can also act as a glucocorticoid-independent negative regulator of transcription, both as an isolated element fused to a minimal promoter and within the context of the proliferin gene promoter. Furthermore, this element is recognized by a factor in mouse fibroblast cell extracts that is distinct from the glucocorticoid receptor and from AP-1, both of which have previously been shown to be able to bind to this site. The ability of this element to repress serum-inducible proliferin promoter activity is dependent on the position of this element with respect to the adjacent serum response region, and on the activity of a positive regulatory element located further upstream in the proliferin promoter.
Hoeppner
M A
MA
Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA.
Mordacq
J C
JC
Linzer
D I
DI
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D011388:Prolactin; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000235:genetics*
D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; D011965:Receptors, Glucocorticoid; D011388:Prolactin; D004247:DNA