Serum from NSAID-treated patients attenuates the capacity of rat leukocytes to synthesize leukotriene B4.
The synthesis of leukotriene B4 by A23187-stimulated rat peritoneal leukocytes was studied in the presence of 0.1% normal human serum, serum from patients treated with NSAIDs for either an inflammatory (rheumatoid arthritis, RA) or a non-inflammatory condition (lumbar disc protrusion, LDP), and serum from RA patients drawn one week after withdrawal from NSAID treatment. The capacity for LTB4 synthesis was significantly lower in the presence of serum from NSAID treated patients: thirty per cent less than observed in presence of normal serum in the RA group, and fifty per cent in the LDP group. When NSAIDs were withdrawn from RA patients, the LTB4 production in presence of serum increased, but was not completely normalized after one week. These results indicate that NSAID treatment may down-regulate the capacity for leukotriene synthesis by an indirect effect.
Gutheil
H
H
Department of Pharmacology, Leo Pharmaceutical Products, Ballerup, Denmark.
Nielsen
O H
OH
Hansen
T M
TM
Thomsen
B S
BS
Ahnfelt-Rønne
I
I
D000328:Adult; D000368:Aged; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000188:drug therapy; D001769:Blood; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D013122:Spinal Diseases / Q000097:blood / Q000188:drug therapy
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007975:Leukotriene B4