title: Stability of the translocation frequency following whole-body irradiation measured in rhesus monkeys.
abstract: Chromosome translocations are persistent indicators of prior exposure to ionizing radiation and the development of 'chromosome painting' to efficiently detect translocations has resulted in a powerful biological dosimetry tool for radiation dose reconstruction. However, the actual stability of the translocation frequency with time after exposure must be measured before it can be used reliably to obtain doses for individuals exposed years or decades previously. Human chromosome painting probes were used here to measure reciprocal translocation frequencies in cells from two tissues of 8 rhesus monkeys (Macaca mulatta) irradiated almost three decades previously. Six of the monkeys were exposed in 1965 to whole-body (fully penetrating) radiation and two were unexposed controls. The primates were irradiated as juveniles to single doses of 0.56, 1.13, 2.00, or 2.25 Gy. Blood lymphocytes (and skin fibroblasts from one individual) were obtained for cytogenetic analysis in 1993, near the end of the animals' lifespans. Results show identical dose-response relationships 28 y after exposure in vivo and immediately after exposure in vitro. Because chromosome aberrations are induced with identical frequencies in vivo and in vitro, these results demonstrate that the translocation frequencies induced in 1965 have not changed significantly during the almost three decades since exposure. Finally, our emerging biodosimetry data for individual radiation workers are now confirming the utility of reciprocal translocations measured by FISH in radiation dose reconstruction.
authors.affiliation: Lawrence Livermore National Laboratory, University of California 94551, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Lucas
authors.forename: F S
authors.initials: FS
authors.lastname: Hill
authors.forename: C E
authors.initials: CE
authors.lastname: Burk
authors.forename: A B
authors.initials: AB
authors.lastname: Cox
authors.forename: T
authors.initials: T
authors.lastname: Straume
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008253:Macaca mulatta; D017785:Photons; D014178:Translocation, Genetic; D014916:Whole-Body Irradiation; D014965:X-Rays