title: Reason for non-aphid transmissibility in a strain of Kalanchoë mosaic potyvirus.
abstract: Kalanchoë mosaic potyvirus (KMV) is transmitted by aphids in a nonpersistent manner. After multiple mechanical inoculations, a non-aphid-transmissible (NAT) variant appeared. Addition of purified helper component (HC) from potato virus Y-infected plants did not restore the aphid transmissibility of purified KMV-NAT. Comparison of the nucleotide sequence in the N-terminal half of the coat protein gene of KMV-NAT with that of an aphid-transmissible (AT) KMV strain revealed a single nucleotide difference (G to A). This nucleotide change turns a glycine residue (G) in the KMV-AT isolate into a glutamic acid residue (E) in the KMV-NAT isolate, abolishing the Asp-Ala-Gly (DAG) sequence, which has previously been shown to be essential for aphid transmission. The experiments show that non-aphid transmissibility is most likely caused by the change in the DAG sequence.
authors.affiliation: Department of Plant Pathology and Pest Management, Danish Institute of Plant and Soil Science, Lyngby, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Husted
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000821:virology*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics; D010944:Plants / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D004279:DNA, Viral; D009842:Oligopeptides