title: Disposition and metabolism of 2,3,7,8-tetrachlorodibenzofuran by channel catfish (Ictalurus punctatus).
abstract: The disposition and metabolism of 2,3,7,8-tetrachlorodibenzofuran (TCDF) was was investigated in channel catfish (Ictalurus punctatus) in order to better understand the metabolic and physiological factors that modulate the fate of this extremely toxic compound in channel catfish compared to other species. The fish were dosed orally with [3H]TCDF (1 microgram/kg); tissue were harvested at 3, 7, and 14 days for radioassay. The body burden of TCDF equivalents in catfish at 3 days was 0.36 microgram/kg, which was decreasing with a half-life of 3.6 days. Catfish muscle showed a relatively low capacity to accumulate and retain TCDF, accounting, at 3 days, for only 19.0% of the body burden of TCDF equivalents (half-life in muscle, 5.0 days). Catfish liver, on the other hand, showed a high capacity to accumulate and metabolize TCDF and to secrete TCDF metabolites into the bile. At 3 days, the concentrations of TCDF equivalents in liver and bile were, respectively, 5.7 ng/g liver (19% of the body burden) and 129 ng/ml bile. However, the concentration of TCDF equivalents in liver decreased with a half-life of 1.8 days to 0.04 ng/g (2.0% of the body burden) at 14 days. Thus, the capacity of catfish liver to retain TCDF decreased dramatically as the body burden decreased. The data suggest that the low affinity of lipid poor catfish muscle for TCDF may allow catfish liver to accumulate a concentration of TCDF sufficient to induce the metabolism of this compound by liver monooxygenases. The major TCDF metabolites found in catfish liver and bile were, respectively, 4-OH-TCDF and TCDF-4-O-glucuronide.
authors.affiliation: Environmental Toxicology and Chemistry Laboratory, Great Lakes Center for Environmental Research and Education, State University of New York College at Buffalo 14222, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Steward
authors.forename: R
authors.initials: R
authors.lastname: Maslanka
authors.forename: K G
authors.initials: KG
authors.lastname: Stuart
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: H C
authors.initials: HC
authors.lastname: Sikka
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000493:pharmacokinetics*; D001646:Bile / Q000378:metabolism; D004785:Environmental Pollutants / Q000493:pharmacokinetics*; D006207:Half-Life; D007059:Ictaluridae / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D001572:Benzofurans; D004785:Environmental Pollutants; C014211:2,3,7,8-tetrachlorodibenzofuran