title: Role for CD30 in HIV expression.
abstract: CD30 is a member of the tumor necrosis factor (TNF)-receptor superfamily, whose ligand (CD30L) has been identified on B cells, activated macrophages and a subset of activated T cells. We show here that infection in vitro with human immunodeficiency virus (HIV) of CD4+ T-cell clones generated from HIV-seronegative individuals can enhance the expression of CD30, which often preceeds and is associated with the death of clonal T cells. Furthermore, cross-linking CD30 with an agonistic CD30-specific monoclonal antibody potentiated HIV replication induced by an insolubilized anti-CD3 antibody in T-cell lines generated from HIV-infected individuals. More importantly, paraformaldehyde-fixed CD8+ T-cell clones expressing CD30L enhanced HIV replication in anti-CD3-stimulated allogeneic or autologous HIV-infected CD4+ T-cell lines and such a potentiating effect was inhibited by an anti-CD30L antibody. The anti-CD30L antibody also exerted a suppressive effect on the spontaneous HIV replication occurring in lymph node cells, freshly derived from an HIV-seropositive patient showing CD30 expression in B cells and in a proportion of CD8+ T lymphocytes. Thus, CD30 triggering may play an important role in both HIV replication and the death of HIV-infected CD4+ T cells.
authors.affiliation: Institute of Internal Medicine and Immunoallergology, University of Florence, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Romagnani
authors.forename: F
authors.initials: F
authors.lastname: Annunziato
authors.forename: R
authors.initials: R
authors.lastname: Manetti
authors.forename: F
authors.initials: F
authors.lastname: Almerigogna
authors.forename: R
authors.initials: R
authors.lastname: Biagiotti
authors.forename: M G
authors.initials: MG
authors.lastname: Giudizi
authors.forename: A
authors.initials: A
authors.lastname: Ravina
authors.forename: V
authors.initials: V
authors.lastname: Giannò
authors.forename: L
authors.initials: L
authors.lastname: Tomasévic
authors.forename: E
authors.initials: E
authors.lastname: Maggi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D053285:CD30 Ligand; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000821:virology; D016923:Cell Death / Q000276:immunology; D002460:Cell Line; D006678:HIV / Q000187:drug effects*; D015658:HIV Infections / Q000276:immunology; D006801:Humans; D017730:Ki-1 Antigen / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011485:Protein Binding / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000911:Antibodies, Monoclonal; D053285:CD30 Ligand; D017730:Ki-1 Antigen; D008024:Ligands; D008562:Membrane Glycoproteins; C505774:TNFSF8 protein, human