Monoclonal antibodies against human blastocysts.
A panel of monoclonal antibodies against the implanting mouse blastocyst was tested for cross-reactivity to human blastocysts. The monoclonal antibodies were produced by intrasplenic immunization with living mouse blastocysts or egg-cylinders. Among the 13 anti-mouse blastocyst antibodies checked, 6 ones detected antigen epitopes also in human blastocysts. If the mouse-human cross-reacting antibodies detect similar antigens in blastocysts of the two species, the results imply that anti-mouse blastocyst antibodies with specific properties could be used for studying human blastocysts. This procedure opens for a way to obtain monoclonal antibodies for analysing human blastocysts.
Nilsson
B O
BO
Department of Human Anatomy, Biomedical Center, Uppsala, Sweden.
Hjortberg
M
M
Olovsson
M
M
Sjögren
A
A
Hamberger
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001755:Blastocyst / Q000166:cytology / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice
D000911:Antibodies, Monoclonal; D000941:Antigens