Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.
Twenty-five kilobases of Pseudomonas plasmid CAM-OCT DNA encoding a DNA repair gene(s) was cloned into the broad-host-range vector pVK100. The presence of the cloned genes increased the isolation frequency of Pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after UV irradiation. Ethyl lactate-utilizing strains expressed a novel intracellular hydrolase.
McBeth
D L
DL
Department of Microbiology and Immunology, CUNY Medical School/Sophie Davis School of Biomedical Education, New York 10031, USA.
Hauer
B
B
D000473:Alkanes / Q000378:metabolism; D002164:Camphor / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D016296:Mutagenesis; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014466:Ultraviolet Rays
D000473:Alkanes; D002164:Camphor