Infection and replication of a planthopper transmitted virus-rice stripe virus in rice protoplasts.
Rice stripe virus (RSV), a planthopper-transmitted virus, was inoculated into rice protoplasts, and a one-step growth curve was determined. The amount of virus in the protoplasts decreased following the inoculation, and then increased after 8 h. The replication of RSV reached its peak 20 h after inoculation. RSV replication in the inoculated protoplasts was further demonstrated by electron microscopy.
Yang
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W
Institute of Microbiology, Chinese Academy of Sciences, Beijing, PRC.
Wang
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X
Wang
S
S
Yie
Y
Y
Tien
P
P
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D007303:Insect Vectors / Q000821:virology; D007313:Insecta / Q000821:virology; D012275:Oryza / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D011523:Protoplasts / Q000821:virology; D012328:RNA Viruses / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D014764:Viral Proteins