title: Applications of laser scanning microscopy for analysis of aquatic microhabitats.
abstract: Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages.
authors.affiliation: Section of Microbiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Ghiorse
authors.forename: D N
authors.initials: DN
authors.lastname: Miller
authors.forename: R L
authors.initials: RL
authors.lastname: Sandoli
authors.forename: P L
authors.initials: PL
authors.lastname: Siering
mesh_terms: D000818:Animals; D001345:Autoradiography; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D018441:Biofilms; D017404:In Situ Hybridization, Fluorescence; D008277:Magnesium Oxide / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D015347:RNA Probes / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D011817:Rabbits; D014871:Water Microbiology
chemical_list: D015347:RNA Probes; D012336:RNA, Ribosomal, 16S; D008277:Magnesium Oxide