Construction of a ferritin-deficient mutant of Campylobacter jejuni: contribution of ferritin to iron storage and protection against oxidative stress.
The ferritin-encoding gene (cft) of Campylobacter jejuni was cloned and sequenced. The nucleotide sequence of cft had a 501 bp open reading frame for a protein with 167 amino acids and a predicted molecular mass of 19 180 Da, and showed a high similarity to that of Helicobacter pylori and Escherichia coli ferritin genes. To determine the biological function of ferritin in C. jejuni, a ferritin-deficient mutant was constructed. The growth of ferritin-deficient strain SNA 1 was clearly inhibited under iron deprivation. The ferritin-deficient mutant was more sensitive to killing by H2O2 and paraquat than the isogenic parent strain. These findings demonstrate that ferritin in C. jejuni makes a significant contribution to both iron storage and protection from intracellular iron overload, and resulting iron-mediated oxidative stress.
Wai
S N
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Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan. amako@bact.med.kyushu-u.ac.jp
Nakayama
K
K
Umene
K
K
Moriya
T
T
Amako
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron