Genetic properties of Mycoplasma mycoides subspecies mycoides LC strains isolated from a goat in Japan in 1991.
Field isolated Mycoplasma strains LG1 and SF1 were isolated from the lung and leg joint fluid of a goat, respectively in Japan in 1991. These strains were closely related and had the following biochemical properties: glucose was metabolized, arginine and urea were not hydrolyzed, tetrazolium was reduced, phosphatase produced, and casein and coagulated serum were digested. Serologically, these strains cross-reacted with three Mycoplasma species [M. mycoides subsp. mycoides (Mmm), M. mycoides subsp. capri, and M. sp. Group 7]. From the above results, the field isolates were considered to be Mmm. In addition, DNA-DNA relatedness between the field isolates and other Mycoplasma species called the "mycoides cluster" was determined, and a recently developed PCR method was also used for mycoplasmal identification. From these results, the field isolates were identified as Mmm LC type.
Kobayashi
H
H
National Institute of Animal Health, Ibaraki, Japan.
Munthali
G
G
Kaiga
M
M
Morozumi
T
T
Mitani
K
K
Ito
N
N
Shiono
H
H
Yamamoto
K
K
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D017931:DNA Primers; D004269:DNA, Bacterial; D015511:Goat Diseases; D006041:Goats; D008168:Lung / Q000382:microbiology; D009176:Mycoplasma mycoides / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013582:Synovial Fluid / Q000382:microbiology
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial