Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames.
We report the sequence of a 15.5 kb DNA segment located near the left telomere of chromosome XV of Saccharomyces cerevisiae. The sequence contains nine open reading frames (ORFs) longer than 300 bp. Three of them are internal to other ones. One corresponds to the gene LGT3 that encodes a putative sugar transporter. Three adjacent ORFs were separated by two stop codons in frame. These ORFs presented homology with the gene CPS1 that encodes carboxypeptidase S. The stop codons were not found in the same sequence derived from another yeast strain. Two other ORFs without significant homology in databases were also found. One of them, O0420, is very rich in serine and threonine and presents a series of repeated or similar amino acid stretches along the sequence.
Gamo
F J
FJ
Instituto de Investigaciones Biomédicas, CSIC Unidad de Bioquímica y Genética de Levaduras, Madrid, Spain.
Lafuente
M J
MJ
Casamayor
A
A
Ariño
J
J
Aldea
M
M
Casas
C
C
Herrero
E
E
Gancedo
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D016615:Telomere / Q000235:genetics
D018388:Codon, Terminator; D017931:DNA Primers; D004271:DNA, Fungal; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D002268:Carboxypeptidases; C019836:CPS1 protein, S cerevisiae