Ovine 11 beta-hydroxysteroid dehydrogenase type 2 gene predicts a protein distinct from that deduced by the cloned kidney cDNA at the C-terminus.
The gene encoding ovine 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) was cloned and characterized. This gene consists of five exons and is greater than 4 kb in length. It contains an open reading frame of 1215 bp, which encodes a protein of 404 amino acids with a predicted MW of 44 kDa. The deduced ovine 11 beta-HSD2 protein displays over 78% sequence identity to those of the human, rabbit, rat, and mouse. However, this differs from the published sequence of ovine kidney 11 beta-HSD2 cDNA which predicts a protein of 427 amino acids. Sequence alignment indicated that this discrepancy is attributed to two single nucleotide omissions in the published cDNA sequence which resulted in a shift in the open reading frame at the codon for residue 358. Therefore, the present results have provided conclusive evidence that the primary structure of 11 beta-HSD2 protein is well conserved between the sheep and the other four mammals. Moreover, Northern blot analysis of total RNA samples from 15 peripheral tissues and seven brain regions of the mature fetal sheep revealed that the expression of 11 beta-HSD2 gene is highly tissue-specific in that it is only expressed in the kidney and adrenal gland, and at a much lower abundance in the testis, colon and placenta. The cloning of the sheep 11 beta-HSD2 gene should facilitate future studies on the regulation of 11 beta-HSD2 gene expression during fetal development in a mammalian model.
Campbell
L E
LE
Lawson Research Institute, St. Joseph's Hospital, Department of Ob/Gyn, University of Western Ontario, London, Canada.
Yu
M
M
Yang
K
K
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D014018:Tissue Distribution
D018076:DNA, Complementary; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases