title: Extracellular ATP increases cytosolic calcium in cultured rat renal arterial smooth muscle cells.
abstract: 1. Experiments were conducted on cultured renal arterial smooth muscle cells to determine the ability of extracellular ATP to alter cytosolic calcium concentration and to determine the mechanisms by which this effect occurs. 2. ATP (100 mumol/L) caused the fluorescence ratio of fura-2 to increase from a control value of 1.06 +/- 0.05 to 2.06 +/- 0.13 (P < 0.01) before stabilizing at a sustained level of 1.35 +/- 0.04 (n = 8; P < 0.05). 3. Removal of extracellular calcium from the bathing medium resulted in an attenuation of the initial response to 100 mumol/L ATP with cell fluorescence increasing from 1.16 +/- 0.18 to 1.44 +/- 0.18 ratio units (n = 5). Furthermore, the initial increase in fluorescence ratio rapidly declined to 1.02 +/- 0.06, indicating that an influx of extracellular calcium is required to sustain the increase in fura-2 fluorescence. 4. Depletion of intracellular calcium pools with thapsigargin prevented the increase in fura-2 fluorescence evoked by ATP. 5. These data suggest that ATP-mediated increases in cytosolic calcium in cultured renal arterial smooth muscle cells involve calcium release from the thapsigargin-sensitive, intracellular pool in conjunction with calcium influx from the extracellular medium.
authors.affiliation: Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
authors.forename: E W
authors.initials: EW
authors.lastname: Inscho
authors.forename: T P
authors.initials: TP
authors.lastname: Belott
authors.forename: M J
authors.initials: MJ
authors.lastname: Mason
authors.forename: J B
authors.initials: JB
authors.lastname: Smith
authors.forename: L G
authors.initials: LG
authors.lastname: Navar
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012077:Renal Artery / Q000166:cytology / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D002118:Calcium; D016257:Fura-2