The effect of the crp genotypes on the transformation efficiency in Escherichia coli.
We have found that a significant difference exists in transformation efficiency between the crp+/crp- isogenic pair of strains of Escherichia coli, with the efficiency being much higher in crp- than in crp+. The ratio of transformation efficiency between crp+ and crp- strains depends very little on the plasmid size. This observation suggests that the difference of the transformation efficiency is due to mechanisms other than a crp-regulated endonuclease. The crp gene is one of the first specific genes that have been shown to affect transformation efficiency.
Umemoto
A
A
Department of Public Health, Kansai Medical University, Osaka, Japan.
Morita
M
M
Nakazono
N
N
Sugino
Y
Y
D017104:Bacteriophage M13 / Q000235:genetics; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000235:genetics; D010957:Plasmids; D014162:Transfection; D014169:Transformation, Bacterial; D014170:Transformation, Genetic
D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein