Cloning of Murine T Cells
T-cell clones have been extremely useful in studying the cellular arm of the mammalian immune response. A method for generating homogeneous long-term, antigen-specific cultures of murine T cells is discussed, with emphasis on obtaining CD4(+) T-cell clones. Some procedures and assays that will be helpful in characterizing the T-cell clones are also included.
Gaur
A
Department of Medicine, Stanford University Medical Center, Stanford, California, 94305