title: High-performance liquid chromatographic method for the determination of RGH-5002 in human, rabbit and rat plasma.
abstract: A sensitive, accurate and reproducible high performance liquid chromatography (HPLC) procedure for the analysis of RGH-5002 in biological fluids was developed. After a single step liquid-liquid extraction at pH 9 using n-hexane, RGH-5002 and internal standard were eluted from a Hypersil Si column with 5 mM ammonium acetate-methanol-acetonitrile (0.5:45:50 v/v/v) at 28 degrees C. The method could accurately detect 5 ng ml-1 of RGH-5002.
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Chemical Works of Gedeon Richter Ltd, Budapest, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Gémesi
authors.forename: M
authors.initials: M
authors.lastname: Kapás
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D009125:Muscle Relaxants, Central / Q000097:blood*; D010880:Piperidines / Q000097:blood*; D011817:Rabbits; D051381:Rats; D015203:Reproducibility of Results; D012821:Silanes / Q000097:blood*
chemical_list: D009125:Muscle Relaxants, Central; D010880:Piperidines; C098298:RGH 5002; D012821:Silanes