Molecular cloning of a genomic DNA for enolase from Aspergillus oryzae.
We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5'- and 3'- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.
Machida
M
M
Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Gonzalez
T V
TV
Boon
L K
LK
Gomi
K
K
Jigami
Y
Y
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D016385:TATA Box
D004269:DNA, Bacterial; D010751:Phosphopyruvate Hydratase