Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones.
We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.
Petit
J
J
Laboratorie de Biochimie et Biologie Moléculaire, Université de Bordeaux II, France.
Boisseau
P
P
Evans
K
K
Christie
S
S
Gosden
C
C
Muir
W
W
St Clair
D
D
Porteous
D
D
Arveiler
B
B
D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004251:DNA Transposable Elements; D015723:Gene Library; D005820:Genetic Testing; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004251:DNA Transposable Elements