Aspergillus fumigatus peptides differentially express Th1 and Th2 cytokines.
Relevant allergens from Aspergillus fumigatus associated with allergic bronchopulmonary aspergillosis (ABPA) have been cloned and expressed. The pathogenesis of ABPA probably depends on specific cytokines and immunoglobulins secreted by lymphocytes on stimulation with different epitopes of those allergens. In the present study, we synthesized peptides of 12-16 amino acids from the sequence of Asp fI and compared their immunological responses in four mice strains (BALB/c, C57BL/6, AKR, and CBA). Of the five peptides studied for their cytokine profile, one showed a clear Th1, whereas another showed a Th2 response. The remaining three peptides varied in their immunoreactivity. The results suggest that a number of epitopes of diverse activities are present in individual molecules and may be involved in the pathogenesis of ABPA through differential cytokine secretions.
Kurup
V P
VP
Department of Medicine, Medical College of Wisconsin, Milwaukee, USA.
Hari
V
V
Guo
J
J
Murali
P S
PS
Resnick
A
A
Krishnan
M
M
Fink
J N
JN
D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D052179:Antigens, Plant; D001232:Aspergillus fumigatus / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D000908:Antibodies, Fungal; D052179:Antigens, Plant; D016207:Cytokines; D005656:Fungal Proteins; D007070:Immunoglobulin A; D007376:Interleukin-2; D015848:Interleukin-5; D016753:Interleukin-10; C036196:MITF protein, Aspergillus restrictus; D015847:Interleukin-4; D007371:Interferon-gamma; D012260:Ribonucleases