Collagen synthesized in fluorocarbon polymer implant in the rabbit cornea.
The integration of microporous polymer into tissues is of great interest for the production of keratoprosthetic devices. Our previous studies showed functional differentiated cells and collagen synthesis in the pore of an expanded polytetrafluoroethylene implant. This study identifies and quantifies collagen types synthesized in the implant. Expanded polytetrafluoroethylene polymers were implanted in the rabbit corneas. The collagen extracted from the polymer and implanted stroma after 1, 3 and 6 months was quantified by measuring hydroxyproline. The relative proportions of collagen types were determined by densitometric analysis after SDS-PAGE. The collagen-to-protein ratio in the polymer increased from 0.22 to 0.70 between the first and third month after implantation becoming similar to control cornea. So that of the protein and collagen densities in the polymer and implanted stroma were similar to the control from the third month. The collagen synthesized in the polymer was mainly type I (87%) plus a small amount of type III (8%) 1 month after implantation. The collagen distribution from the third month after implantation was similar to that of the controls and remained constant thereafter in the polymer implant and in the implanted stroma. Immunogold labelling techniques confirmed these results. Implantation of this PTFE disc induced no obvious modification of the corneal stroma, confirming that this polymer is a good interface that is compatible with the native corneal stroma. The keratocytes in this polymer rapidly adopted a corneal phenotype, distinct from the dermal or scaring phenotype as shown by the collagen types produced in the implant.
Drubaix
I
I
INSERM U86, Laboratoire de Biochimie de la Cornée, Paris, France.
Legeais
J M
JM
Malek-Chehire
N
N
Savoldelli
M
M
Ménasche
M
M
Robert
L
L
Renard
G
G
Pouliquen
Y
Y
D000818:Animals; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis* / Q000648:ultrastructure; D003315:Cornea / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D008854:Microscopy, Electron; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D011817:Rabbits; D013997:Time Factors
D005136:Eye Proteins; D011138:Polytetrafluoroethylene; D003094:Collagen; D006909:Hydroxyproline