L-Arginine infusion after ischaemia-reperfusion of rat kidney enhances lipid peroxidation.
To assess the role of superoxide (O2-) and nitric oxide (NO) in ischaemic-reperfusion-induced acute renal failure, we investigated whether an activation of the L-arginine-NO pathway contributes to ischaemia-reperfusion-induced kidney membrane peroxidation by measurement of 4-hydroxynonenal (HNE) content in anaesthetized rats submitted to acute renal ischaemia. Following ischaemia-reperfusion injury, renal blood flow (RBF) was significantly reduced, while renal vascular resistance was significantly increased. Infusion of neither L-arginine nor D-arginine led to a recovery of RBF. L-Arginine, but not D-arginine, caused a significant increase in HNE accumulation in the ischaemic kidney. L-Arginine infusion enhanced the degree of lipid peroxidation afforded by ischaemia-reperfusion injury in the kidney suggesting that products of the endogenous L-arginine-NO pathway may react with O2- to initiate lipid peroxidation.
Cristol
J P
JP
INSERM U58, Montpellier, France.
Thiemermann
C
C
Guérin
M C
MC
Torreilles
J
J
de Paulet
A C
AC
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism / Q000494:pharmacology*; D006224:Cricetinae; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012079:Renal Circulation / Q000187:drug effects; D015427:Reperfusion Injury / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014655:Vascular Resistance / Q000187:drug effects
D000447:Aldehydes; D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D001120:Arginine; C027576:4-hydroxy-2-nonenal