Coinfection with human herpesvirus 6 variants A and B in lung tissue.
Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to contain HHV-6 DNA. A total of 22 lung tissue samples showed coinfections with HHV-6 variants A and B, 2 had only HHV-6 variant A DNA, and 10 had only HHV-6 variant B DNA. The prevalences of coinfections in lung tissues from healthy controls (54% coinfected) and in those from bone marrow transplant patients with pneumonia (67% coinfected) were similar. These data indicate that coinfections of HHV-6 variants A and B commonly occur in lung tissues of healthy and diseased individuals.
Cone
R W
RW
Department of Laboratory Medicine, University of Washington, Seattle, USA.
Huang
M L
ML
Hackman
R C
RC
Corey
L
L
D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D006566:Herpesviridae Infections / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008168:Lung / Q000821:virology*; D008969:Molecular Sequence Data; D011024:Pneumonia, Viral / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral