In vivo absorption spectrum of disulphonated aluminium phthalocyanine in a murine tumour model.
The absorption spectrum of aluminum phthalocyanine with an average disulphonation of 2.1 (hereafter called disulphonated aluminum phthalocyanine, A1S2Pc) was measured in vivo in a murine tumour model by means of time-resolved reflectance. Mice bearing the L1210 leukaemia were administered 2.5 or 5 mg/kg body weight (b.w.) of A1S2Pc intraperitoneally. Reflectance measurements were performed in the 650-695 nm range before and 1, 4 and 7 h after the drug administration. Fitting of the data with the diffusion theory allowed us to assess the absorption coefficient in both conditions (i.e. before and after). As a difference between the latter and the former data, the in vivo absorption spectrum of A1S2Pc was evaluated. 1 h after the administration of 2.5 mg/kg b.w. A1S2Pc, the absorption peak was centred at 685 nm, red-shifted about 15 nm with respect to the spectrum in aqueous solution. For the lower dose, the absorption line shapes 4 and 7 h after the administration remained very similar. The red shift of the absorption spectrum is consistent with the therapeutic efficacy of the photodynamic therapy which was measured at 672, 685 and 695 nm, and proved to be maximum at 685 nm for both the L1210 leukaemia and the MS-2 fibrosarcoma. With the higher drug dose, the absorption spectra taken from different animals showed significant differences. In particular, in some mice the line shape was similar to that measured with 2.5 mg/kg b.w., while in other subjects it showed a broadening or a second peak at shorter wavelengths. Measurements on some animals were performed also 18 and 24 h after the injection of 5 mg/kg b.w., leading to no time evolution or to a progressive line shape narrowing.
Cubeddu
R
R
C.E.Q.S.E.-C.N.R., Politecnico di Milano, Italy.
Canti
G
G
Musolino
M
M
Pifferi
A
A
Taroni
P
P
Valentini
G
G
D000818:Animals; D001128:Argon; D004195:Disease Models, Animal; D005260:Female; D005354:Fibrosarcoma / Q000188:drug therapy; D007211:Indoles / Q000737:chemistry* / Q000627:therapeutic use; D007834:Lasers; D007939:Leukemia L1210 / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009942:Organometallic Compounds / Q000737:chemistry* / Q000627:therapeutic use; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000737:chemistry* / Q000627:therapeutic use; D013053:Spectrophotometry
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; D001128:Argon; C064331:aluminum phthalocyanine disulfonate