Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.
The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gene express phosphoserine aminotransferase activity, verifying the function of this gene.
Metcalf
W W
WW
Department of Microbiology, University of Illinois, Urbana 61801, USA. bill_metcalf@qms1.life.uiuc.edu
Zhang
J K
JK
Shi
X
X
Wolfe
R S
RS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D019605:Euryarchaeota / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D017021:Methanosarcina barkeri / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000637:Transaminases / Q000235:genetics*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000637:Transaminases; C022393:phosphoserine aminotransferase