Release of TNF alpha in the rat hippocampus following epileptic seizures and excitotoxic neuronal damage.
We studied the production of tumor necrosis factor alpha (TNF alpha) and interleukin-6 (IL-6) 2 and 7 days following status epilepticus (SE), induced in rats by intra-amygdala injection of kainate. At day 2 the release of both cytokines by hippocampal slices prepared from epileptic animals was increased compared to controls, whereas at day 7 only TNF alpha secretion was enhanced. Since SE-induced neuronal damage is probably due to excitotoxicity, we investigated the effects of agonists of glutamate receptors on TNF alpha release in organotypic hippocampal cultures. A correlation was found between the damage intensity and the release of TNF alpha, suggesting production of this cytokine by macrophagic microglia. We propose a role for TNF alpha and IL-6 in the adaptive phenomena which follow severe limbic seizures.
de Bock
F
F
Laboratoire de Médecine Expérimentale, INSERM U249, CNRS UPR9008, Institut de Biologie, Montpellier, France.
Dornand
J
J
Rondouin
G
G
D000679:Amygdala / Q000187:drug effects; D000818:Animals; D016923:Cell Death; D006624:Hippocampus / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007608:Kainic Acid; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000139:chemically induced / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007608:Kainic Acid