Elution of viruses by ionic and nonionic surfactants.
The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when combinations of surfactants and binding membranes were matched (i.e., ionic-ionic or nonionic-nonionic).
Fujito
B T
BT
Thomas Jefferson High School for Science and Technology, Alexandria, Virginia 22312, USA.
Lytle
C D
CD
D000327:Adsorption; D001435:Bacteriophages / Q000302:isolation & purification*; D017830:Octoxynol / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol