Recombinant microtubule-associated protein 2c reduces the dynamic instability of individual microtubules.
The effects of purified recombinant microtubule-associated protein 2c (rMAP2c) on the dynamic instability of microtubules were examined by direct observation of individual microtubules in vitro by video-enhanced differential interference contrast light microscopy. Microtubules were grown in the absence or presence of varying concentrations of rMAP2c and were analyzed to determine growth rates, shortening rates, and the frequencies of conversion between growing and shortening phases. We found rMAP2c to stabilize microtubules dramatically. The most notable effect is a reduction in both the frequency of catastrophes (transitions from growth to shortening) and the mean length of shortening events: no microtubule catastrophes were observed at concentrations of rMAP2c as low as 1.06 microM in a solution of 10 microM tubulin. Even at lower rMAP2c concentrations, there is a marked stabilizing effect. As the concentration of rMAP2c increases, average growth rates increase slightly, shortening rates decrease, and the frequency of rescues (transitions from shortening to growth) increases significantly. Together, these changes in parameters produce a population of extremely stable microtubules in the presence of rMAP2c. This stabilization is consistent with a structural role for MAP2c during early postnatal neural development.
Gamblin
T C
TC
Department of Neuroscience, University of Virginia, Charlottesville 22908, USA.
Nachmanoff
K
K
Halpain
S
S
Williams
R C
RC
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002696:Chlamydomonas; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video; D008869:Microtubule-Associated Proteins / Q000378:metabolism* / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D011994:Recombinant Proteins; D014404:Tubulin; D017239:Paclitaxel