Studies on the ileum-contracting mechanisms and identification as a complement C3a receptor agonist of oryzatensin, a bioactive peptide derived from rice albumin.
Oryzatensin (Gly-Tyr-Pro-Met-Tyr-Pro-Leu-Pro-Arg) is an ileum-contracting and immunostimulating peptide derived from rice albumin. The mechanisms for the ileal contraction that it induces, consisting of rapid and slow components, were examined. The rapid contraction was mediated by histamine release and the slow contraction by a prostaglandin E2-like substance, judging from the effects of various pharmacological inhibitors and antagonists on ileal contraction and titration of histamine release. The contractile profile was very similar to that of human complement C3a(70-77), which is the COOH-terminal octapeptide of C3a and has, although less potent, qualitatively the same biological activities as C3a. Oryzatensin showed homology with C3a(70-77) and affinity for C3a receptors (IC50 = 44 microM) by radioreceptor assay. This is the first report of a food-derived bioactive peptide acting through complement C3a receptors.
Takahashi
M
M
Department of Food Science and Technology, Kyoto University, Japan.
Moriguchi
S
S
Ikeno
M
M
Kono
S
S
Ohata
K
K
Usui
H
H
Kurahashi
K
K
Sasaki
R
R
Yoshikawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015232:Dinoprostone / Q000502:physiology; D006168:Guinea Pigs; D006636:Histamine Release; D006801:Humans; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012275:Oryza; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011951:Receptors, Complement / Q000819:agonists* / Q000187:drug effects / Q000378:metabolism
D008565:Membrane Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D010940:Plant Proteins; D011951:Receptors, Complement; C085567:complement C3a receptor; C091249:oryzatensin; D015232:Dinoprostone