Flow cytometric analysis of the platelet surface area and surface density of glycoprotein IIb-IIIa of unactivated human platelets of various sizes.
Large (L) activated platelets exhibit greater aggregability and express more activated glycoprotein IIb-IIIa (GPIIb-IIIa) per cell and per unit surface area than small (S) activated platelets. We studied the binding of CD61 monoclonal antibody to GPIIb-IIIa on resting platelets and developed a new method for determining platelet surface area by flow cytometry. Using this method, we found that resting L platelets contain two times more GPIIb-IIIa per cell than S platelets but the same amount of GPIIb-IIIa per unit surface area. The data suggest that the greater aggregability of L platelets is likely to be due to increased activation and/ or expression of GPIIb-IIIa rather than to elevated density of unactivated GPIIb-IIIa on resting L platelets.
Leytin
V
V
Department of Hematology, Meir Hospital, Kfar-Saba, Israel.
Shapiro
H
H
Novikov
I
I
Radnay
J
J
D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D048429:Cell Size; D005434:Flow Cytometry; D006801:Humans; D010974:Platelet Aggregation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*
D019039:Platelet Glycoprotein GPIIb-IIIa Complex