Heliquinomycin, a new inhibitor of DNA helicase, produced by Streptomyces sp. MJ929-SF2 I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
Heliquinomycin was isolated as a part of a program designed to find inhibitors of DNA helicase from microbial sources. It was purified from the culture broth of Streptomyces sp. MJ929-SF2 by solvent extraction and serial chromatographies of centrifugal partition chromatography, Sephadex LH-20 and Capcell Pak C18 (HPLC). The isolated red powder was analyzed to have the molecular formula of C33H30O17. It inhibited partially purified DNA helicase from HeLa cell in a non-competitive manner with the inhibition constant (Ki) of 6.8 mM. Heliquinomycin exhibited biological activity against microorganisms including MRSA, and cultured cell lines.
Chino
M
M
Institute of Microbial Chemistry, Tokyo, Japan.
Nishikawa
K
K
Umekita
M
M
Hayashi
C
C
Yamazaki
T
T
Tsuchida
T
T
Sawa
T
T
Hamada
M
M
Takeuchi
T
T
D000818:Animals; D001419:Bacteria / Q000187:drug effects; D016227:Benzoquinones / Q000302:isolation & purification / Q000494:pharmacology; D004265:DNA Helicases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D013141:Spiro Compounds / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016227:Benzoquinones; D004791:Enzyme Inhibitors; D013141:Spiro Compounds; C101772:heliquinomycin; D004265:DNA Helicases