Evidence and nature of a novel thiol-oxidase in human platelets.
The study, addressed to understand whether or not human platelets possess a unique thiol-oxidase whose activity could be modulated by signalling pathway initiated upon the activation of Receptor-'C'k revealed the existence of disulphide-dependent oxidation within these cells and this phenomenon was regulated by Receptor-'C'k-dependent generation of second messengers especially phosphatidic acid(PA); cAMP and cGMP. Purification of this activity revealed the existence of 47 kDa protein having thiol-oxidase activity. Keeping in view these results we propose that the existence of this novel 47 kDa Thiol-oxidase within human platelets may provide a 'crucial switch' for the regulation of Receptor-'C'k-dependent mevalonate pathway in human platelets.
Kaul
D
D
Experimental Medicine and Biotechnology Department, Postgraduate Institue of Medical Education and Research, Chandigarh, India.
Dhawan
V
V
Kaur
M
M
D001792:Blood Platelets / Q000201:enzymology*; D002784:Cholesterol / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D006801:Humans; D008798:Mevalonic Acid / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010712:Phosphatidic Acids / Q000378:metabolism; D011973:Receptors, LDL / Q000378:metabolism; D015398:Signal Transduction; D013438:Sulfhydryl Compounds / Q000378:metabolism
D010712:Phosphatidic Acids; D011973:Receptors, LDL; D013438:Sulfhydryl Compounds; D002784:Cholesterol; D000242:Cyclic AMP; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; C112161:thiol oxidase, 47 kDa; D006152:Cyclic GMP; D008798:Mevalonic Acid