title: Effect of gonadotropin-releasing hormone on estrogen receptor messenger ribonucleic acid level in perifused pituitary cells.
abstract: 1. We examined the potential effect of GnRH pulses on pituitary estrogen receptor mRNA level. 2. The treatment of perifused pituitary cell aggregates with four hourly pulses of GnRH (10nM/1 min/h) resulted in a marked increase in the steady-state level of ER mRNA (25% vs unstimulated control, n = 3). 3. No changes were observed for the LH beta mRNA. Data suggest, for the first time, that a cross-talk between the GnRH and nuclear ER may occur in the gonadotrope cells.
authors.affiliation: Laboratorie d' Endocrinologie Moléculaire, URA CNRS 256, Rennes, France.
authors.forename: F
authors.initials: F
authors.lastname: Demay
authors.forename: C
authors.initials: C
authors.lastname: Tiffoche
authors.forename: M L
authors.initials: ML
authors.lastname: Thieulant
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone