A somatodendritic distribution of Rab11 in rabbit brain neurons.
Comparisons of subcellular targeting in neurons and epithelial cells have led to suggestions that apical epithelial antigens localize to axons and nerve terminals. We have studied the distribution in brain of the small GTP-binding protein, Rab11, which is localized to apical vesicular populations in epithelial cells. Sections of rabbit brain were examined by immunohistochemistry using a monoclonal antibody against rabbit Rab11. Rab11 immunoreactivity was present exclusively in neurons. In all regions examined, including forebrain, cerebellum, thalamus and brainstem, Rab11 immunoreactivity was observed in cell bodies and dendrites. No staining was observed in hippocampal neurons. These results indicate that the distribution of Rab11 does not support previous suggestions that apical epithelial markers should localize to axons and synaptic endings.
Sheehan
D
D
Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912-3175, USA.
Ray
G S
GS
Calhoun
B C
BC
Goldenring
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001923:Brain Chemistry / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000032:analysis*; D015151:Immunoblotting; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D011817:Rabbits; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
D000911:Antibodies, Monoclonal; D019204:GTP-Binding Proteins; D009419:Nerve Tissue Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins