Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus.
To evaluate the usefulness of a DNA vaccine for chickens, we constructed a plasmid vector expressing the Newcastle disease virus F protein (NDV-F) under the control of the human cytomegalovirus immediate early enhancer and chicken beta-actin gene promoter. One-week-old chickens injected intramusculary with the circular plasmid DNA did not produce significant levels of antibody against NDV-F. However, two of five birds injected with the linearized plasmid DNA produced high levels of the antibody. Moreover, four of five birds injected with a mixture of the linearized-plasmid and Lipofectin produced the antibody efficiently. At 9 weeks post-injection, chickens were challenged with the velogenic NDV Sato strain. These chickens that had the antibody against NDV-F were protected from lethal NDV challenge. These results demonstrate that the DNA vaccine conferred efficient protection against the disease.
Sakaguchi
M
M
Chemo-Sero Therapeutic Research Institute, Kikuchi Research Center, Kumamoto, Japan.
Nakamura
H
H
Sonoda
K
K
Hamada
F
F
Hirai
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D009521:Newcastle Disease / Q000517:prevention & control*; D010957:Plasmids; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics / Q000592:standards; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000592:standards
D000914:Antibodies, Viral; D004274:DNA, Recombinant; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines