Quantification of polycyclic aromatic hydrocarbons and polychlorinated dibenzo-p-dioxins in human serum by combined micelle-mediated extraction (cloud-point extraction) and HPLC.
Quantification of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated dibenzo-p-dioxins (PCDDs) usually requires preconcentration and cleanup prior to analysis. These procedures often involve using large amounts of toxic organic solvents. The sample preparation from serum is even more complex because of the coextraction of lipids and other nonpolar serum components. We describe the unprecedented use of cloud-point extraction to preconcentrate, extract, and clean up PAHs and PCDDs from human serum using the nonionic surfactant Triton X-100. The samples were analyzed by high-performance liquid chromatography with ultraviolet detection. The phase separation was induced by the addition of salt to the micellar serum solutions. The surfactant-rich phase was treated with acetonitrile and water to precipitate and remove some of the unwanted substances in the serum sample extract without significantly affecting the recoveries of the analytes. The favorable characteristics of cloud-point extraction discussed here strengthen its potential use as an alternative to other techniques of separation.
Sirimanne
S R
SR
Centers for Disease Control and Prevention (CDC), U.S. Department of Health and Human Services, Atlanta, Georgia 30341-3724, USA.
Barr
J R
JR
Patterson
D G
DG
Ma
L
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004147:Dioxins / Q000097:blood*; D006801:Humans; D008823:Micelles; D017830:Octoxynol; D011084:Polycyclic Aromatic Hydrocarbons / Q000097:blood*; D012965:Sodium Chloride
D004147:Dioxins; D008823:Micelles; D011084:Polycyclic Aromatic Hydrocarbons; D012965:Sodium Chloride; D017830:Octoxynol; C020887:dibenzo(1,4)dioxin