Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora.
Full length cDNA clones of flavonoid 3',5'-hydroxylase, dihydroflavonol 4-reductase and flavonoid 3-glucosyltransferase were cloned from petals of Gentiana triflora. Their sequences were homologous to counterparts from other plants. Flavonoid 3',5'-hydroxylase and flavonoid 3-glucosyltransferase were enzymatically characterized by expressing cDNAs in heterologous expression systems.
Tanaka
Y
Y
Institute for Fundamental Research, Suntory Ltd., Osaka, Japan.
Yonekura
K
K
Fukuchi-Mizutani
M
M
Fukui
Y
Y
Fujiwara
H
H
Ashikari
T
T
Kusumi
T
T
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000096:biosynthesis*; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D018744:DNA, Plant; D015870:Gene Expression; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000872:Anthocyanins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; C084258:flavonoid 3',5'-hydroxylase; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase