title: Molecular cloning and tissue-specific expression of mouse kidney 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
abstract: A 1932 bp cDNA clone encoding a novel isozyme of 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (PFK-2/ FBPase-2) was isolated from a mouse kidney cDNA library. The sequence encodes 519 amino acids and, based on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK-2/FBPase-2 gene B with an extended version of exon 15. Northern blot analysis indicated that this clone corresponds to an 8 kb mRNA expressed in multiple tissues, with the strongest signal in kidney, and detects several additional transcripts which may be alternatively spliced from gene B.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: R S
authors.initials: RS
authors.lastname: Batra
authors.forename: R
authors.initials: R
authors.lastname: Brown
authors.forename: G K
authors.initials: GK
authors.lastname: Brown
authors.forename: I W
authors.initials: IW
authors.lastname: Craig
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D005091:Exons; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis* / Q000737:chemistry; D015723:Gene Library; D007527:Isoenzymes / Q000096:biosynthesis; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D010732:Phosphofructokinase-1 / Q000096:biosynthesis* / Q000737:chemistry; D025481:Phosphofructokinase-2; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D007527:Isoenzymes; D025481:Phosphofructokinase-2; D010732:Phosphofructokinase-1; D006597:Fructose-Bisphosphatase