Identification of the GABAA receptor alpha-subunit mRNA in rat intestine.
We investigated the expression of gamma-aminobutyric acid type A (GABAA) receptor alpha 1-, alpha 2- and alpha 3-subunit mRNAs in the rat intestine using reverse transcription and polymerase chain reaction. alpha 1- and alpha 3-, but not alpha 2-, subunit mRNAs were amplified from the proximal intestine, ileum, and colon. In-situ hybridization studies demonstrated the expression of alpha 1-subunit mRNA by myenteric neurons. GABA may be active via the GABAA receptor in the enteric nervous system.
Zeiter
D K
DK
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia, PA 19104, USA.
Li
X
X
Broussard
D L
DL
D000818:Animals; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism*
D012333:RNA, Messenger; D011963:Receptors, GABA-A