Immunogenicity and safety of recombinant Helicobacter pylori urease in a nonhuman primate.
Groups of squirrel monkeys (Saimiri spp.), predetermined to be free of Helicobacter infections in the gastric mucosa, were immunized orally with 0.5-4.5 mg of Helicobacter pylori recombinant urease (rUrease) and 25-500 micrograms of Escherichia coli heat-labile enterotoxin (LT) adjuvant. Oral immunization with rUrease resulted in a markedly elevated serum immunoglobulin G (IgG) antibody response with peak levels at 45 days after immunization. No significant gastric inflammation or cytotoxicity was evident in rUrease immunized monkeys as determined by light and electron microscopy. Twenty-five micrograms of LT was a sufficient and safe adjuvant dosage, whereas higher dosages resulted in diarrhea and lethargy. Animals developed a serum IgG antibody response to LT that did not impede the production of anti-rUrease antibody levels. The results of this investigation indicate that rUrease is immunogenic in a nonhuman primate.
Stadtländer
C T
CT
Greenville Hospital System/Clemson University Biomedical Cooperative, Department of Microbiology, South Carolina 29634, USA.
Gangemi
J D
JD
Khanolkar
S S
SS
Kitsos
C M
CM
Farris
H E
HE
Fulton
L K
LK
Hill
J E
JE
Huntington
F K
FK
Lee
C K
CK
Monath
T P
TP
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000276:immunology*; D001706:Biopsy; D004768:Enterotoxins / Q000008:administration & dosage / Q000276:immunology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G / Q000097:blood*; D008854:Microscopy, Electron; D012453:Saimiri; D013270:Stomach / Q000166:cytology / Q000187:drug effects; D014510:Urease / Q000009:adverse effects / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; C027538:heat-labile enterotoxin, E coli; D014510:Urease