Role of interleukin-1 beta, interleukin-6 and macrophage inflammatory protein-1 beta in prostaglandin-E2-induced hyperthermia in rats.
The purpose of this study was to investigate the role of pyrogenic cytokines, such as IL-1 beta, IL-6 and MIP-1 beta, in the mechanisms underlying the hyperthermic response of rats to central injection of PGE2. Thus, specific murine neutralizing antibodies against these cytokines were micro-injected directly into the anterior hypothalamic, preoptic area (AH/POA) of unrestrained rats just before intracerebroventricular injection of PGE2. The significant hyperthermia induced by PGE2 was markedly suppressed by micro-injection of anti-IL-6 and partially attenuated by anti-IL-1 beta. However, the micro-injection of anti-MIP-1 beta failed to alter the hyperthermic response. The results indicate that PGE2-induced hyperthermia is presumably mediated through actions of IL-6 on the thermosensitive cells of the AH/POA and confirm that distinct and alternate pathways exist in the rat brain for the induction of fever.
Fernández-Alonso
A
A
Department of Pharmacology, Pediatrics and Radiology, School of Medicine, University of Seville, Spain.
Benamar
K
K
Sancibrián
M
M
López-Valpuesta
F J
FJ
Miñano
F J
FJ
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology; D054407:Chemokine CCL4; D015232:Dinoprostone / Q000502:physiology*; D005334:Fever / Q000503:physiopathology*; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D015850:Interleukin-6 / Q000276:immunology / Q000502:physiology*; D019402:Macrophage Inflammatory Proteins; D008297:Male; D008845:Microinjections; D015846:Monokines / Q000276:immunology / Q000502:physiology*; D009500:Neutralization Tests; D011301:Preoptic Area; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D054407:Chemokine CCL4; D007375:Interleukin-1; D015850:Interleukin-6; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D015232:Dinoprostone