[Effects of chronic ethanol feeding on lipid composition of rat liver plasma membrane--changes of membrane by acute ethanol loading or its withdrawal in vivo].
The effects of chronic ethanol feeding on lipid composition of rat liver plasma membrane were studied by following ethanol loading or its withdrawal in vivo. Male Wistar rats were pair-fed by a liquid diet containing ethanol as 36% of energy or an isocarolic amounts of glucose for 6 weeks. Chronic ethanol feeding resulted in an increase of cholesterol content and the cholesterol/phospholipid molar ratio in liver plasma membrane compared with pair-fed control rats. Acute ethanol (3 g/kg body weight) orally loading to rats fed ethanol chronically did not change any lipid composition of liver plasma membrane. However, withdrawal of ethanol for 2 days led cholesterol content and the cholesterol/phospholipid molar ratio of liver plasma membrane to be normal values. These data indicated that chronic ethanol feeding produced membrane alterlation that induced resistance to ethanol-induced membrane structural disordering (membrane tolerance), and that this alterlation resulted in a homeoviscous adaptation of liver plasma membrane.
Yamashita
T
T
Department of Public Health, Osaka City University Medical School, Japan.
Warashina
M
M
Kondo
K
K
Tanaka
T
T
Goto
Y
Y
Kawai
S
S
Monna
T
T
Matsui
T
T
Toda
T
T
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D000431:Ethanol / Q000009:adverse effects*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
D008563:Membrane Lipids; D000431:Ethanol