Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.
cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2-terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein (approximately 34 kDa) compared to mouse. PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death.
Takayama
S
S
Bumham Institute, La Jolla, California 92037, USA.
Kochel
K
K
Irie
S
S
Inazawa
J
J
Abe
T
T
Sato
T
T
Druck
T
T
Huebner
K
K
Reed
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006801:Humans; D006822:Hybrid Cells; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012377:Rodentia; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
C091732:BCL2-associated athanogene 1 protein; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D014157:Transcription Factors