Nucleotide sequence of a plasmid pCL2.1 from Lactococcus lactis ssp. lactis ML8.
The nucleotide sequence of a small cryptic plasmid, pCL2.1, from Lactococcus lactis ssp. lactis ML8 was determined. Sequence analysis of the pCL2.1 revealed that it contained 2112 bp, 33.9% GC, and two open reading frames that encoded polypeptides of 26 and 14 kDa. In vitro transcription-translation of the pCL2.1 confirmed the existence of two polypeptides. Based on sequence homology, it is deduced that the ORF2 product functions in plasmid replication by a rolling circle mechanism.
Chang
H C
HC
Department of Food Science and Technology, Seoul National University, 441-744 Suwon, Korea.
Choi
Y D
YD
Lee
H J
HJ
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*
D004269:DNA, Bacterial