title: Feeding heated soyabean flour increases the density of B and T lymphocytes in the small intestine of calves.
abstract: The effect of dietary antigens on the gut morphology and density of immune cells was studied in preruminant calves fed milk substitutes containing skim milk powder (SMP), non-antigenic hydrolysed soya protein isolate (HSPI) or antigenic heated soyabean flour (HSF) as their main protein source for 3 months. The height and perimeter of proximal jejunum villi were highest in the calves fed SMP and lowest in those fed HSF (P < 0.05). In contrast, the crypt depth and perimeter were apparently not influenced by the dietary treatments studied. This morphological alteration was associated with a dramatic infiltration of the lamina propria by B and T lymphocytes in the calves fed HSF (P < 0.01). Increased B cell density was essentially accounted for by IgA-, IgG1- and IgG2-bearing cells. The density of CD2-positive T lymphocytes increased (P < 0.01) in the jejunal lamina propria of HSF calves, involving helper (CD4+) and suppressor-cytotoxic (CD8+) T cell subsets. The density of gamma-delta (WC1+) T cells also increased (P < 0.01). The major change concomitantly observed in the villus epithelium was an increased density of CD8+ cells (P < 0.05) and WC1 + cells (P < 0.01).
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire du Jeune Ruminant, Rennes, France. Ialles@roazhon.inra.fr
authors.forename: J P
authors.initials: JP
authors.lastname: Lallès
authors.forename: D
authors.initials: D
authors.lastname: Dréau
authors.forename: F
authors.initials: F
authors.lastname: Féménia
authors.forename: A L
authors.initials: AL
authors.lastname: Parodi
authors.forename: R
authors.initials: R
authors.lastname: Toullec
mesh_terms: D000821:Animal Feed; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005433:Flour / Q000032:analysis*; D006358:Hot Temperature / Q000627:therapeutic use*; D007421:Intestine, Small / Q000276:immunology*; D007583:Jejunum / Q000276:immunology; D008297:Male; D013025:Glycine max / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*