title: Simultaneous determination of spironolactone and its metabolites in human plasma.
abstract: This study describes a specific, precise, sensitive and accurate method for determination of unchanged spironolactone and its major active metabolites in human plasma. After one-step liquid-liquid extraction, analysis of the parent drug and its metabolites was performed in one chromatographic run, using a high performance liquid chromatography (HPLC) method with a programmed switchover of the UV wavelength. Spironolactone and 7 alpha-thiomethyl-spironolactone were detected at 245 nm, while canrenone and internal standard were detected at 280 nm. The column used was an S5 ODS2 (500 mm x 4.6 mm i.d.). The mobile phase was a mixture of acetonitrile-aqueous orthophosphoric acid (pH 3.4). Chromatographic separations were performed at 5 degrees C. The standard curves were linear over the range 10-400 ng ml-1 for spironolactone and 10-600 ng ml-1 for 7 alpha-thiomethyl-spironolactone and canrenone. The precision and accuracy of the method were confirmed by relative standard deviations below 10% for different concentrations, except for the concentration equal to the quantitation limit, where these parameters ranged from 12-15%. The recovery was above 80% for all investigated compounds and for the internal standard. The assay proved to be suitable for pharmacokinetic studies of spironolactone.
authors.affiliation: Medical Center of Postgraduate Education, Department of Biopharmaceutics, Bydgoszcz, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Jankowski
authors.forename: A
authors.initials: A
authors.lastname: Skorek-Jankowska
authors.forename: H
authors.initials: H
authors.lastname: Lamparczyk
mesh_terms: D002192:Canrenone / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013148:Spironolactone / Q000031:analogs & derivatives / Q000097:blood*
chemical_list: D013148:Spironolactone; C046564:7-thiomethylspirolactone; D002192:Canrenone