title: Expression of rat neuronal nitric oxide synthase in Saccharomyces cerevisiae.
abstract: The rat neuronal nitric oxide synthase (nNOS) cDNA was expressed in Saccharomyces cerevisiae under the control of a hybrid PGK/GAL promoter, PAL. Galactose induction resulted in the production of a soluble 160 kDa protein that was recognised by anti-neuronal NOS antibody. NOS activity was detected by the conversion of [3H]arginine to [3H]citrulline and required the addition of the cofactors, calmodulin and tetrahydrobiopterin. The activity was inhibited by the arginine analogues NG-nitro-L-arginine and NG-nitro-L-arginine methyl ester.
authors.affiliation: Delta Biotechnology Limited, Nottingham, UK.
authors.forename: S
authors.initials: S
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Tasab
authors.forename: J E
authors.initials: JE
authors.lastname: Ogden
authors.forename: D J
authors.initials: DJ
authors.lastname: Ballance
authors.forename: M J
authors.initials: MJ
authors.lastname: Powell
mesh_terms: D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D018076:DNA, Complementary / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D019001:Nitric Oxide Synthase