The mouse Kin-17 gene codes for a new protein involved in DNA transactions and is akin to the bacterial RecA protein.
We have sought to characterize the molecular basis of the sensitivity to ionising radiation and to identify the genes involved in the cellular response of mammalian cells to such radiation. Using the Escherichia coli model, we tested the hypothesis that functional domains of RecA protein are represented in proteins of mammalian cells. We review here the results obtained in the detection of nuclear proteins of mammalian cells that are recognized by anti-RecA antibodies. We have called them kin proteins. Kin proteins likely play a role in DNA metabolism. We summarize the cloning of the mouse Kin-17 cDNA and our work on the identification and preliminary characterisation of the biochemical properties of mouse kin17 protein, a new nuclear protein able to recognize bent DNA and suspected to be involved in illegitimate recombination. We briefly describe our latest experiments on the molecular characterisation of the mouse Kin-17 gene. Finally, we discuss the properties of kin17 protein and the possible participation of kin17 protein in DNA transactions like transcription or recombination.
Tissier
A
A
Département de Radiobiologie et de Radiopathologie, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France.
Kannouche
P
P
Biard
D S
DS
Timchenko
T
T
Mazin
A
A
Araneda
S
S
Allemand
I
I
Mauffrey
P
P
Frelat
G
G
Angulo
J F
JF
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002875:Chromosomes; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000378:metabolism*; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005796:Genes; D008322:Mammals; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011506:Proteins / Q000276:immunology; D011938:Rec A Recombinases / Q000276:immunology*
D000906:Antibodies; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D011506:Proteins; D004247:DNA; D011938:Rec A Recombinases