Analysis of Mutator-induced mutations in the iojap gene of maize.
The recessive nuclear mutation iojap (ij) in maize produces striped plants with normal chloroplasts in green sectors and poorly developed chloroplasts in the white sectors. The ij mutation is also characterized by an array of additional phenotypic affects which suggests a pivotal role for Ij in chloroplast development. The Ij gene from maize has been isolated; however, the sequence has not provided information on the action of the Ij gene product. As an extension of the analysis of the Ij gene we have generated a number of independent transposon insertion mutations using the Robertson's Mutator (Mu). Insertions were found to be distributed throughout the 5' half of the gene and all showed a similar striping pattern to that of the original ij mutation, ij-ref. While several of the new mutant alleles produced ij-homologous transcripts none produced detectable levels of the Ij polypeptide product. Chloroplast development in some cells appears to be able to proceed in the absence of the Ij polypeptide.
Byrne
M
M
Cooperative Research Center for Plant Science, Australian National University, Canberra, Australia.
Taylor
W C
WC
D000483:Alleles; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002736:Chloroplasts / Q000378:metabolism; D002874:Chromosome Mapping; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements; D010940:Plant Proteins