title: Purification and properties of a basic endo-1,3-beta-glucanase from rice (Oryza sativa L.).
abstract: A 1,3-beta-glucanase purified from rice grain is a 33 kDa monomer with a pI of > or = 10.4. The enzyme was determined to be an endo-1,3-beta-glucanase (EC 3.2.1.39) by end product analysis using Laminaria digitata laminarin as substrate. Its amino-terminal amino acid sequence revealed strong homology to an endo-1,3-beta-glucanase from barley.
authors.affiliation: National Hokkaido Agricultural Experiment Station, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Kaku
authors.forename: N
authors.initials: N
authors.lastname: Shibuya
mesh_terms: D000595:Amino Acid Sequence; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity; D047071:beta-Glucans
chemical_list: D005936:Glucans; D011134:Polysaccharides; D047071:beta-Glucans; C038459:curdlan; C008247:laminaran; D004693:Glucan Endo-1,3-beta-D-Glucosidase