Oxidative damages by iron-chelate complexes depend on the interaction with the target molecules.
To elucidate that in iron-catalyzed oxidative damage the interaction of iron complex with the target molecules is important, the oxidative damage to plasmid DNA, protein and fatty acid has been compared using iron-chelate complexes with nitrilotriacetic acid (nta), citric acid, ethylenediamine-N,N'-diacetic acid (edda) and diethylenetriamine-N,N,N',N",N"-pentaacetic acid (dtpa). In the presence of hydrogen peroxide, plasmid pBR322 strand breaks occurred in the order of Fe-edda > Fe-citrate > Fe-nta > > Fe-dtpa. However, fragmentation of bovine serum albumin and diene conjugation of linoleic acid micelle occurred in the order of Fe-nta > Fe-edda > > Fe-citrate > Fe-dtpa = O, which were similar to hydroxyl radical production by these iron complexes and H2O2. Bleomycin-detectable free radical-promoting irons in these iron complexes were about 85% of iron in Fe-nta, Fe-citrate and Fe-edda, and only about 33% in Fe-dtpa. Not only hydroxyl radical productivity and free radical-promoting iron content in iron complex, but also the interaction of the complex with the target molecules determines the iron-catalyzed oxidative damage.
Asaumi
A
A
Department of Pathology, Okayama University Medical School, Japan.
Ogino
T
T
Akiyama
T
T
Kawabata
T
T
Okada
S
S
D000818:Animals; D001761:Bleomycin; D002417:Cattle; D019343:Citric Acid / Q000494:pharmacology; D004249:DNA Damage / Q000235:genetics; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008722:Methods; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D004369:Pentetic Acid / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000187:drug effects
D005290:Ferric Compounds; D007502:Iron Chelating Agents; D017382:Reactive Oxygen Species; D001761:Bleomycin; D012710:Serum Albumin, Bovine; D019343:Citric Acid; C026058:EDDA; D004369:Pentetic Acid; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate