title: Rapid detection of Mycobacterium avium in stool samples from AIDS patients by immunomagnetic PCR.
abstract: Direct PCR detection of bacteria in clinical samples is often hindered by the presence of compounds that inhibit the PCR. To improve and accelerate the diagnosis of Mycobacterium avium-M. intracellulare complex infections, an immunomagnetic PCR (IM-PCR) assay was developed. This IM-PCR procedure combines the separation of mycobacteria by antimycobacterial monoclonal antibody coupled to magnetic beads with an M. avium-M. intracellulare complex-specific PCR protocol based on 16S rRNA gene sequences. As few as 10 M. avium bacilli were detected in spiked human stool samples, a clinical specimen usually refractory to conventional PCR analysis, by the IM-PCR method. Moreover, M. avium organisms were detected in about 24 h in 18 of 22 culture-confirmed fecal samples from AIDS patients. This IM-PCR protocol should allow for the rapid and sensitive detection of M. avium isolates in clinical specimens.
authors.affiliation: Division of Bacterial Products, Food and Drug Administration, Bethesda, Maryland 20892, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: G H
authors.initials: GH
authors.lastname: Bai
authors.forename: C F
authors.initials: CF
authors.lastname: von Reyn
authors.forename: P
authors.initials: P
authors.lastname: Marino
authors.forename: M J
authors.initials: MJ
authors.lastname: Brennan
authors.forename: N
authors.initials: N
authors.lastname: Gine
authors.forename: S L
authors.initials: SL
authors.lastname: Morris
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology*; D017931:DNA Primers; D005243:Feces / Q000382:microbiology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D017931:DNA Primers