cDNA sequence, map, and expression of the murine homolog of GTBP, a DNA mismatch repair gene.
DNA mismatch repair genes (MMR) have been actively studied in humans for their role in cancer susceptibility. Recently, GTBP, a new member of the MMR gene family, has been cloned and found to encode a 160-kDa protein that forms a heterodimer with hMSH2. Our group has isolated and characterized the mouse homolog of GTBP. The transcript, termed Gtmbp (G/T mismatch-binding protein), encodes a protein highly similar to its human counterpart. The gene is markedly conserved across species and maps to the distal portion of mouse chromosome 17, in a region homologous to human 2p. One Gtmbp-related sequence maps to proximal mouse chromosome 4. Studies of the expression of Gtmbp suggest that its regulated transcription is tightly linked to the level of DNA replication, consistent with the protein's DNA proofreading function.
Corradi
A
A
Department of Biological and Technological Research (DIBIT), San Raffaele Scientific Institute, via Olgettina 58, Milan, 20132, Italy.
Croci
L
L
Stayton
C L
CL
Gulisano
M
M
Boncinelli
E
E
Consalez
G G
GG
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004260:DNA Repair; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C102997:Msh6 protein, mouse