title: Promotion of synthetic amyloid beta-peptide fibrillization by cell culture media and cessation of fibrillization by serum.
abstract: Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid beta 1-40 and beta 1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat-inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid beta fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.
authors.affiliation: New York State Institute for Basic Research in Developmental Disabilities, Department of Pathological Neurobiology, Staten Island 10314, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wegiel
authors.forename: A
authors.initials: A
authors.lastname: Chauhan
authors.forename: H M
authors.initials: HM
authors.lastname: Wisniewski
authors.forename: J
authors.initials: J
authors.lastname: Nowakowski
authors.forename: K C
authors.initials: KC
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Le Vine
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D006801:Humans; D013997:Time Factors
chemical_list: D016564:Amyloid beta-Protein Precursor; D001798:Blood Proteins; D003470:Culture Media