Effects of different exposure times to tobacco smoke intoxication on carboxyhemoglobin and hepatic enzymate activities in mice.
The aim of this work was to determine the effects of different exposure times to smoke on carboxyhemoglobin (HbCO) and hepatic enzymate activities in order to adapt a tobacco smoke intoxication model in mice. Mice were exposed to tobacco smoke for various durations of either 2 (group S2), 4 (group S4), 8 (group S8), or 31 days (group S31) using the Hamburg II machine. Controls (nonexposed animals) were used under the same experimental conditions. On the 2nd, 4th, 8th, and 31st day, mice were sacrificed by decapitation, and blood carboxyhemoglobin level and hepatic enzymate activities catalysed by CYP 450 families were measured. Our data with regard to the exposed group indicated first that HbCO was significantly increased after 4 or 8 days of exposure and decreased after 31 days compared to controls (where HbCO was constant for the duration of the 31 days) and second, the enzymate activities were significantly higher during the period of exposure. In conclusion, a 4- and 8-day exposure period with eight cigarettes per day seems to be the model of tobacco smoke intoxication in mice to be chosen.
Attolini
L
L
Medical and Clinical Pharmacology Laboratory, Faculty of Medicine of Marseille-ER 311, France.
Gantenbein
M
M
Villard
P H
PH
Lacarelle
B
B
Catalin
J
J
Bruguerolle
B
B
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002263:Carboxyhemoglobin / Q000032:analysis*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
D012906:Smoke; D003577:Cytochrome P-450 Enzyme System; D002263:Carboxyhemoglobin; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating