title: The disulfide folding pathway of tick anticoagulant peptide (TAP), a Kunitz-type inhibitor structurally homologous to BPTI.
abstract: The pathway of oxidative folding of tick anticoagulant peptide (TAP, 60 amino acids and three disulfides) has been analyzed by characterization of the acid and iodoacetate trapped folding intermediates. The results reveal a high degree of heterogeneity of the one- and two-disulfide intermediates and the presence of three-disulfide scrambled species along the folding pathway. The picture of TAP folding differs significantly from the well-documented case of bovine pancreatic trypsin inhibitor (BPTI), despite the fact that both proteins share close structural homology in term of 3-D conformation and disulfide pattern.
authors.affiliation: Pharmaceuticals Research Laboratories, Ciba-Geigy Ltd., Basel, Switzerland.
authors.forename: J Y
authors.initials: JY
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D007611:Aprotinin / Q000737:chemistry; D060829:Arthropod Proteins; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry*; D005978:Glutathione / Q000494:pharmacology; D019791:Guanidine; D006146:Guanidines; D006629:Hirudins / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D014269:Trifluoroacetic Acid / Q000494:pharmacology
chemical_list: D060829:Arthropod Proteins; D004220:Disulfides; D006146:Guanidines; D006629:Hirudins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D015842:Serine Proteinase Inhibitors; C063967:tick anticoagulant peptide; D008623:Mercaptoethanol; D007611:Aprotinin; D014269:Trifluoroacetic Acid; D005978:Glutathione; D019791:Guanidine