Formation of DNA-adducts by selected sex steroids in rat liver.
1. We are reporting investigations into the potential of the steroid hormones chlormadinone acetate (CMA), cyproterone acetate (CPA), ethinylestradiol (EE2) gestodene (GEST), megestrol acetate (MGA), norethisterone acetate (NET-Ac), estradiol (E2), and progesterone (P) to form DNA-adducts in rat liver in vivo. 2. Compound-related DNA-adduct spots were detected in male and female rat liver following CMA, CPA, and MGA using the 32P-postlabeling-technique. Substance-specific DNA-adducts were also observed in male rats after administration of E2. The other compounds showed no DNA-adduct formation. After treatment with CMA, CPA or MGA, the relative adduct labeling (RAL) differed sex- and substance-specifically.
Feser
W
W
Research Laboratories, Schering Aktiengesellschaft, Berlin, Germany.
Kerdar
R S
RS
Blode
H
H
Reimann
R
R
D000818:Animals; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification; D018736:DNA Adducts / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010761:Phosphorus Radioisotopes; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013256:Steroids / Q000633:toxicity*
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D013256:Steroids; D004247:DNA