Induction of capacitation in human spermatozoa in vitro by an endometrial sialic acid-binding protein.
A Ca(2+)-dependent sialic acid-binding protein (SABP) of human endometrium, which specifically bound to human sperm head plasma membrane in vitro, was found to increase the percentage motility and acrosome-reacted pattern of uncapacitated spermatozoa. The protein was synthesized in the endometrium and secreted into the uterine fluid. This intra-uterine factor, which is apparently advantageous in vitro in inducing human sperm capacitation, may play a significant role in promoting the post-release maturation of ejaculated spermatozoa by enhancing 45Ca uptake into spermatozoa by a pathway which is insensitive to calcium-channel blockers. However, the 45Ca uptake could be enhanced on exposure to the divalent cation ionophore A23187 and inhibited in the presence of the calmodulin inhibitor trifluoperazine. The SABP also induces an increase in intracellular Ca2+ in spermatozoa, as seen by FURA-2 AM studies. Furthermore, overlay studies show human SABP to be a Ca(2+)-binding protein. The data presented here suggest that SABP induces in-vitro sperm capacitation and the subsequent acrosome reaction by increasing intracellular Ca2+ concentration.
Banerjee
M
M
Indian Institute of Chemical Biology, Calcutta, India.
Chowdhury
M
M
D000373:Agglutinins / Q000494:pharmacology* / Q000502:physiology; D001693:Biological Transport, Active / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000494:pharmacology / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002463:Cell Membrane Permeability / Q000187:drug effects; D004717:Endometrium / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D012794:Sialic Acids / Q000378:metabolism; D015398:Signal Transduction; D013075:Sperm Capacitation / Q000187:drug effects* / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D014268:Trifluoperazine / Q000494:pharmacology
D000373:Agglutinins; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D006023:Glycoproteins; D007476:Ionophores; D012794:Sialic Acids; C057612:sialic acid specific agglutinin protein, rat; D014268:Trifluoperazine; D000001:Calcimycin; D002118:Calcium