Multiple mouse chromosomal loci for dynein-based motility.
Dyneins are multisubunit mechanochemical enzymes capable of interacting with microtubules to generate force. Axonemal dyneins produce the motive force for ciliary and flagellar beating by inducing sliding between adjacent microtubules within the axoneme. Cytoplasmic dyneins translocate membranous organelles and chromosomes toward the minus ends of cytoplasmic microtubules. Dynactin is an accessory complex implicated in tethering cytoplasmic dynein to membranous organelles and mitotic kinetochores. In the studies described here, we have identified a number of new dynein genes and determined their mouse chromosomal locations by interspecific backcross analysis. We have also mapped several dynein and dynactin genes cloned previously. Our studies provide the first comprehensive attempt to map dynein and dynactin genes in mammals and provide a basis for the further analysis of dynein function in development and disease.
Vaughan
K T
KT
Cell Biology Group, Worcester Foundation for Biomedical Research, 222 Maple Avenue, Shrewsbury, Massachusetts, 01545, USA.
Mikami
A
A
Paschal
B M
BM
Holzbaur
E L
EL
Hughes
S M
SM
Echeverri
C J
CJ
Moore
K J
KJ
Gilbert
D J
DJ
Copeland
N G
NG
Jenkins
N A
NA
Vallee
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D000072159:Dynactin Complex; D004398:Dyneins / Q000235:genetics*; D005796:Genes / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008868:Microtubule Proteins / Q000235:genetics; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009115:Muridae; D009928:Organ Specificity; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D000072159:Dynactin Complex; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D004398:Dyneins