Determination of dicentrine in rat plasma by high-performance liquid chromatography and its application to pharmacokinetics.
A simple high-performance liquid chromatographic method was developed to study the pharmacokinetics of dicentrine in rat plasma after 10 mg/kg intravenous administration. After addition of an internal standard (coumarin), plasma was deproteinized by acetonitrile for sample clean-up. The drugs were separated on a reversed-phase Nucleosil C18 column (250 x 4 mm I.D., particle size 5 microns) and detected by photodiode-array detection at a wavelength of 308 nm. Acetonitrile-water (35:65, v/v, pH 2.5-2.8, adjusted with orthophosphoric acid) was used as the mobile phase. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration-time curve.
Tsai
T H
TH
Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan.
Tsai
T R
TR
Chou
C J
CJ
Chen
C F
CF
D000317:Adrenergic alpha-Antagonists / Q000097:blood*; D000818:Animals; D001060:Aporphines / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000097:blood*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000317:Adrenergic alpha-Antagonists; D001060:Aporphines; D010975:Platelet Aggregation Inhibitors; C067342:dicentrine