Studies on 13C-phenacetin metabolism. II. A combination of breath test and urine test of in vivo metabolites in the diagnosis of liver disease.
We determined the optimum 13C-labeling position in phenacetin for use in a breath test to diagnose liver disease based on infrared spectroscopy detection of 13CO2 in exhaled air. ([1-13C]Ethoxy)phenacetin gave the best result. This compound was also employed in a urine test using 13C-NMR spectroscopy. In the urine test, healthy subjects gave a higher signal of phenacetin than of its metabolite, phenetidine, whereas in patients with liver disease the situation was the reverse. The combination of the breath and urine tests may be a valuable new tool for the diagnosis of liver disease.
Kajiwara
M
M
Department of Medicinal Chemistry, Meiji College of Pharmacy, Tokyo, Japan.
Okazaki
T
T
Iida
K
K
Narumi
S
S
Hirose
M
M
Ijichi
M
M
Abel
T
T
Hirano
S
S
Iinuma
M
M
D000208:Acute Disease; D001944:Breath Tests; D002247:Carbon Isotopes; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000652:urine; D008107:Liver Diseases / Q000175:diagnosis* / Q000652:urine; D009682:Magnetic Resonance Spectroscopy; D010615:Phenacetin / Q000652:urine*; D013055:Spectrophotometry, Infrared
D002247:Carbon Isotopes; D010615:Phenacetin