Effect of guinea-pig purified immunoglobulin G1 on the responsiveness of tracheal, aortic, vas deferens and ileum smooth muscles.
BACKGROUND
Smooth muscles hyperresponsiveness is a common feature in anaphylaxis and allergic diseases.


OBJECTIVE
The aim of the present work was to investigate the effect of in vitro passive sensitization with highly purified immunoglobulin G1 (IgG1) on the responsiveness of tracheal, aortic, vas deferens and ileum smooth muscles.


METHODS
Firstly, IgG1, obtained from actively sensitized BFA guinea-pigs, was purified by Protein A-Sepharose column and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis analysis. Concentration-response curves to spasmogens (acetylcholine for trachea and vas deferens, noradrenaline for aorta and histamine for ileum) were established before and after in vitro passive sensitization with IgG1.


RESULTS
Contractile responses and maximal contractions were significantly enhanced after passive sensitization for all the organs. Maximal contractions were significantly increased in the trachea (+46.7%), aorta (+51%), vas deferens (+114.2%) and ileum (+117.2%). At the end of the experiments, the application of the sensitizing antigen induced a significant Schultz-Dale reaction of the smooth muscles.


CONCLUSIONS
The present results show that the in vitro application of purified IgG1 can produce non-specific smooth muscle hyperreactivity and hypersensitivity. So, IgG1 can be considered as the main factor involved in the genesis of sensitization-induced hyperresponsiveness, and probably play a great role in hyperreactivity observed during allergic diseases and anaphylaxis.
Bidon
J C
JC
Laboratoire de Physiologie et Pharmacodynamic, Ecole Nationale Vétérinaire, Nantes, France.
Vu
A T
AT
Blin
M
M
Gogny
M
M
Souilem
O
O
Jondet
A
A
D000818:Animals; D000941:Antigens / Q000276:immunology; D001011:Aorta / Q000187:drug effects / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000276:immunology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000276:immunology*; D014132:Trachea / Q000187:drug effects / Q000276:immunology; D014649:Vas Deferens / Q000187:drug effects / Q000276:immunology
D000941:Antigens; D007074:Immunoglobulin G