Experimental evidences for a role of subinhibitory concentrations of rilopirox, nystatin and fluconazole on adherence of Candida spp. to vaginal epithelial cells.
Candidiasis is frequently localized in the mucosal epithelium which covers the vaginal and oral cavity. The pathogenicity of Candida is correlated with its ability to adhere to epithelial cells and this is the resultant of both fungal and host cell properties and their physicochemical interactions. This study was performed to investigate the ability of subinhibitory concentrations (sub-MICs) of rilopirox, a new antimycotic drug, to interfere with the adhesion of Candida albicans, Candida tropicalis and Candida glabrata to human vaginal cells, in comparison with sub-MICs of nystatin and fluconazole. The three drugs are more active on C. albicans, followed by C. tropicalis and, last, C. glabrata, on which fluconazole was inactive (MIC > 24 micrograms/ml). Rilopirox, nystatin and fluconazole have different mechanisms of action, and different molecular weights, so a comparative analysis of data was performed by means of their sub-MICs. On this basis the order of activity was nystatin [symbol: see text] rilopirox > fluconazole. These findings can be of use for optimizing also the posologic design by regarding sub-MICs which are still active in reducing the adhesiveness of Candida to cells of the vaginal mucosa.
Braga
P C
PC
Department of Pharmacology, University of Milan, School of Medicine, Italy.
Maci
S
S
Dal Sasso
M
M
Bohn
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000502:physiology*; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects*; D004847:Epithelial Cells; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009761:Nystatin / Q000494:pharmacology*; D011728:Pyridones / Q000494:pharmacology*; D014621:Vagina / Q000166:cytology / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D011728:Pyridones; D009761:Nystatin; C066307:rilopirox; D015725:Fluconazole