Effect of traY amber mutations on F-plasmid traY promoter activity in vivo.
We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.
Silverman
P M
PM
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA. SILVERMANP@OMRF.UOKHSC.EDU
Sholl
A
A
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018388:Codon, Terminator; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005144:F Factor / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D001426:Bacterial Proteins; D018388:Codon, Terminator; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; C063238:traY protein, E coli; C067119:kilA protein, E coli; D000469:Alkaline Phosphatase