Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.
Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.
Peña
C E
CE
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
Stoner
J E
JE
Hatfull
G F
GF
D000926:Anticodon; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009160:Mycobacteriophages / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic; D016662:Virus Integration / Q000235:genetics*
D000926:Anticodon; D004269:DNA, Bacterial; D012343:RNA, Transfer