Result of oral intake of glucose by healthy subjects and patients with essential hypertension on the binding and degradation of 125I-insulin by erythrocyte receptors.
The work presents the results of researches of binding and degradation of 125I-Insulin by erythrocyte receptors in the patients with essential hypertension and healthy patients after glucose intake. In order to obtain full representation of the pattern of changes the serum IRI and glucose concentrations were assayed. Binding and degradation of 125I-Insulin by erythrocyte receptors were determined with the method described by Gambhir (1977), modified by the authors. The modification consisted in usage of constant concentrations of iodized insulin (0.9 pg/0.1 ml) and bovine insulin (2.4 I.U./0.1 ml). Before administration of glucose and in 30, 60 and 120 minutes after, venous blood was collected from ulnar vein. All examined persons were in sitting position during the trial of glucose intake. Obtained results show, that blood insulin level in the patients with essential hypertension is statistically significantly higher than in healthy persons of similar anthropometric characteristics. Binding of 125I-Insulin to erythrocyte receptors in fasting state is statistically significantly lower. Degradation after glucose intake in the patients shows decreasing tendency, while in healthy persons--growing tendency.
Szcześniak
L
L
University School of Physical Education, Poznań, Poland.
Rychlewski
T
T
Głuszek
J
J
Michocki
P
P
Banaszak
F
F
D000284:Administration, Oral; D000328:Adult; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood; D006973:Hypertension / Q000097:blood*; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin; D005947:Glucose