title: Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains.
abstract: PCR DNA fingerprinting using repetitive intergenic DNA sequences (rep-PCR) was investigated as a means of differentiating between closely related strains of Streptomyces which were, in some cases, indistinguishable by other classification methods. Our results demonstrated that the majority of strains had unique rep-PCR DNA fingerprints and established that the technique could be a very useful tool in rapidly determining strain identity.
authors.affiliation: Department of Soil, Water, and Climate, University of Minnesota, St. Paul 55108, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Sadowsky
authors.forename: L L
authors.initials: LL
authors.lastname: Kinkel
authors.forename: J H
authors.initials: JH
authors.lastname: Bowers
authors.forename: J L
authors.initials: JL
authors.lastname: Schottel
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015203:Reproducibility of Results; D013302:Streptomyces / Q000145:classification* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial