Development of a temperature-inducible expression system for Streptomyces spp.
PCR mutagenesis of a 0.9-kbp fragment, containing a repressor gene, traR, and its target promoter, Ptra, from Streptomyces nigrifaciens plasmid pSN22, produced Streptomyces lividans clones with temperature-inducible Ptra expression. Using the promoterless gene for the thermostable Thermus flavus malate dehydrogenase as an indicator, an induction of enzyme activity of as much as was observed in a temperature shift from 28 to 37 degrees C. Temperature downshift reestablished repression of Ptra, making these promoter cassettes very attractive for the temporally regulated expression of cloned genes in Streptomyces spp.
Kataoka
M
M
International Center for Biotechnology, Osaka University, Japan. mkataoka@nch.go.jp
Tatsuta
T
T
Suzuki
I
I
Kosono
S
S
Seki
T
T
Yoshida
T
T
D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D006358:Hot Temperature; D008291:Malate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics*; D013824:Thermus / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C080512:TraR protein, Agrobacterium tumefaciens; D008291:Malate Dehydrogenase