title: Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway.
abstract: BACKGROUND
  Maltoporin (which is encoded by the lamB gene) facilitates the translocation of maltodextrins across the outer membrane of E. coli. In particular, it is indispensable for the transport of long maltooligosaccharides, as these do not pass through non-specific porins. An understanding of this intriguing capability requires elucidation of the structural basis.
  
  
  RESULTS
  The crystal structures of maltoporin in complex with maltose, maltotriose and maltohexaose reveal an extended binding site within the maltoporin channel. The maltooligosaccharides are in apolar van der Waals contact with the 'greasy slide', a hydrophobic path that is composed of aromatic residues and located at the channel lining. At the constriction of the channel the sugars are tightly surrounded by protein side chains and form an extensive hydrogen-bonding network with ionizable amino-acid residues.
  
  
  CONCLUSIONS
  Hydrophobic interactions with the greasy slide guide the sugar into and through the channel constriction. The glucosyl-binding subsites at the channel constriction confer stereospecificity to the channel along with the ability to scavenge substrate at low concentrations.
authors.affiliation: Department of Structural Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Dutzler
authors.forename: Y F
authors.initials: YF
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Rizkallah
authors.forename: J P
authors.initials: JP
authors.lastname: Rosenbusch
authors.forename: T
authors.initials: T
authors.lastname: Schirmer
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D001692:Biological Transport; D050260:Carbohydrate Metabolism; D018360:Crystallography, X-Ray; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D018272:Porins; D011991:Receptors, Virus / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D009844:Oligosaccharides; D018272:Porins; D011991:Receptors, Virus; C021705:maltooligosaccharides; C016532:maltoporins