title: Expression of IL-3 receptor in testis.
abstract: Interleukin-3 (IL-3) is a potent growth factor for hematopoietic cells. IL-3 transmits its signal through a specific cell surface receptor which consists of a primary binding alpha subunit (IL-3R alpha) and a signaling beta subunit that is shared by the IL-5 and GM-CSF receptors. Here, we describe the expression of the alpha and beta subunit of the IL-3 receptor in mouse testis and testicular cell lines both at nucleic acid and protein levels. Our data indicate that IL-3R alpha is expressed on the cell surface of testicular Leydig cells and a Leydig cell tumor cell line I-10. Expression of the beta subunit mRNA was only faintly detected in testis, but not in I-10 cells. Immunoelectron microscopy has revealed that IL-3R alpha is present on cell membrane and microvilli.
authors.affiliation: Department of Cell Signaling, DNAX Research Institute, Palo Alto, California 94304-1104, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Morikawa
authors.forename: K
authors.initials: K
authors.lastname: Tohya
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D012333:RNA, Messenger / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D016185:Receptors, Interleukin-3