Primary structure of the kinase domain region of rabbit skeletal and cardiac muscle titin.
A 2.3 kb region of rabbit cardiac and skeletal muscle titin has been cloned. The cDNA sequences of the two tissues are identical and show 91% identity on the nucleotide level with the corresponding region of human cardiac muscle titin. On the amino acid level the identity is 96% and similarity is 98%. Alignment of predicted amino acid sequences of several homologous kinase domains reveals that the rabbit titin kinase has all the necessary elements of an active catalytic domain and carries a potential regulatory region on its C-terminal end. The distance of the 2.3 kb contig from the 3' end of the message was determined to be 5.7 kb in both tissues using oligonucleotide directed RNase H cleavage of titin mRNAs. This is essentially identical with the length of the fully sequenced human cardiac titin C-terminal end. It therefore appears unlikely that there are major tissue specific differences in this 8 kb cDNA region which encodes the C-terminus of rabbit skeletal and cardiac titin.
Sebestyén
M G
MG
Department of Meat and Animal Science, Waisman Center, University of Wisconsin-Madison 53705, USA.
Fritz
J D
JD
Wolff
J A
JA
Greaser
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry; D002645:Chickens; D064211:Connectin; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry; D016376:Oligonucleotides, Antisense; D011494:Protein Kinases / Q000096:biosynthesis* / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D016914:Ribonuclease H; D017386:Sequence Homology, Amino Acid
D002148:Calmodulin-Binding Proteins; D064211:Connectin; D009124:Muscle Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C577285:TTN protein, human; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D016914:Ribonuclease H