Immune responses of lambs to the fusion (F) glycoprotein of bovine respiratory syncytial virus expressed on insect cells infected with a recombinant baculovirus.
A group of lambs was immunized with the F protein of bovine respiratory syncytial virus expressed in a baculovirus (Bac-F) and their humoral and cellular immune responses to bovine RSV studied before and after challenge with infectious bovine RSV. Immunization with Bac-F resulted in significant humoral immune responses as measured by virus neutralization and cellular immune responses as measured by lympho-proliferation against inactivated bovine RSV and specific cytotoxicity against autologous targets. Challenge infection with bovine RSV was characterized by significant anamnestic responses in Bac-F immunized lambs and virus shedding in nasal secretions was significantly lower in Bac-F immunized lambs than in control lambs.
Sharma
A K
AK
University of Liverpool, Department of Veterinary Pathology, Leahurst, Neston, Wirral, UK.
Woldehiwet
Z
Z
Walrevens
K
K
Letteson
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D015707:HN Protein; D008297:Male; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D012756:Sheep; D018411:Spodoptera; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D017201:Virus Shedding / Q000276:immunology
D000914:Antibodies, Viral; D015707:HN Protein; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G