Structural organization and chromosomal assignment of the human 14-3-3 eta chain gene (YWHAH).
14-3-3 protein, a brain-specific protein, is thought to be a multifunctional protein involved in the activation of tyrosine and tryptophan hydroxylases, the inhibition or activation of protein kinase C, and the activation of signal transduction. The human 14-3-3 eta chain gene was isolated and its structure was determined. It is composed of two exons separated by one long intron (approximately 8 kb) and spans about 10 kb. A transcription initiation site was identified by a combination of S1 nuclease mapping, primer extension analysis, and RACE methods. In the 5'-flanking region, we found four GC box sequences, four anti-GC box sequences, a TATA box-like sequence, CAAT box-like sequences, a C/EBP element, two AP-2 sequences, an AP-3 sequence, an Oct-6-like sequence, six E boxes, and a CRE sequence. FISH with DNA probes of the human 14-3-3 eta chain gene mapped the 14-3-3 eta chain gene to chromosome 22q12.1-q13.1.
Muratake
T
T
National Saigata Hospital, Niigata Prefecture, Ogata, Nakakubiki, 949-31, Japan.
Hayashi
S
S
Ichikawa
T
T
Kumanishi
T
T
Ichimura
Y
Y
Kuwano
R
R
Isobe
T
T
Wang
Y
Y
Minoshima
S
S
Shimizu
N
N
Takahashi
Y
Y
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D011506:Proteins; C482984:YWHAH protein, human; D014446:Tyrosine 3-Monooxygenase