title: Quality control of pharmaceuticals containing clenbuterol by thermal lens spectrometry.
abstract: An ultrasensitive absorptiometric procedure for the determination of clenbuterol in pharmaceutical preparations was developed. Clenbuterol was diazotized with nitrite and coupled with 1-(naphthyl)ethylenediamine, and the absorbance of the azo dye formed was measured by both spectrophotometry and ultrasensitive thermal lens spectrometry (TLS). The TLS limit of detection was 1.5 ppb, 14-fold lower than with a Hewlett-Packard diode array spectrophotometer. Thus, the TLS procedure can be advantageously applied to quality control of clenbuterol at the individual dose level and in small samples. Repeatability as relative standard deviation was 1.5% (50 ppb, n = 6).
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Burjassot (València), Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Martin Biosca
authors.forename: J J
authors.initials: JJ
authors.lastname: Baeza Baeza
authors.forename: G
authors.initials: G
authors.lastname: Ramis-Ramos
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D002976:Clenbuterol / Q000032:analysis*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013607:Tablets / Q000032:analysis*
chemical_list: D000318:Adrenergic beta-Agonists; D013607:Tablets; D002976:Clenbuterol