The use of a new viability assay to determine the susceptibility of Cryptosporidium and Eimeria sporozoites to respiratory inhibitors and extremes of pH.
A new viability assay for Cryptosporidium and Eimeria sporozoites is described. It involves the use of both acridine orange and bis-benzimide and is more rapid, easier and less subjective than procedures used previously. The assay has been used to investigate the effects of respiratory inhibitors and pH on the sporozoites of C. parvum, C. muris and E. tenella. Neither cyanide nor azide reduced the viability of C. parvum or E. tenella, whereas they had some effect on C. muris. This latter organism, an intracellular parasite of stomach epithelial cells, also differed from the other two in being able to survive pH 2 for as long as 1 h.
Brown
S M
SM
Infection and Immunity, University of Glasgow, UK.
McDonald
V
V
Denton
H
H
Coombs
G H
GH
D000165:Acridine Orange / Q000494:pharmacology; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D003458:Cryptosporidium / Q000187:drug effects* / Q000502:physiology*; D016785:Cryptosporidium parvum / Q000187:drug effects* / Q000502:physiology*; D016786:Eimeria tenella / Q000187:drug effects* / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D011190:Potassium Cyanide / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D019810:Sodium Azide; D013170:Spores / Q000187:drug effects / Q000502:physiology; D013194:Staining and Labeling / Q000379:methods
D001386:Azides; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D012402:Rotenone; D005557:Formaldehyde; D019810:Sodium Azide; D000165:Acridine Orange; D011190:Potassium Cyanide