Pseudomonas aeruginosa clinical isolates: serotypes, resistance phenotypes and plasmid profiles.
78 Pseudomonas aeruginosa strains were isolated from the respiratory tract of 56 patients, 15 of which were affected by cystic fibrosis (CF). The epidemiological typing scheme was based on serotyping, antibiotic resistance pattern and plasmid DNA profile. All strains (except 2 mucoid strains) were typed using a rapid slide O-agglutination technique. Most common serotypes in both group were 0:1, 0:10 and 0:6. Moreover we observed a correlation among 0:12 serotype and CF patients. Plasmid DNA analysis showed that 45.2% (on average) of strains isolated from patients with and w/o CF harboured 1-3 plasmids ranging in size from 1 to 15 Md. Plasmid prevalence was higher in strains isolated from CF patients in specimens collected after antibiotic therapy. A correlation was found between 1 and 1.9 Md plasmids and resistance to aminoglycosides. Our results indicate that the analysis of antibiotic resistance phenotypes combined with plasmid analysis may be useful, in association to serotyping, to characterize the circulation of P. aeruginosa strains and the spread of resistance in these bacteria.
Millesimo
M
M
Department of Clinical and Biological Sciences, University of Turin, San Luigi Hospital, Orbassano, Italy.
de Intinis
G
G
Chirillo
M G
MG
Musso
T
T
Savoia
D
D
D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D006801:Humans; D017720:Molecular Epidemiology; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics; D012141:Respiratory Tract Infections / Q000382:microbiology*; D012703:Serotyping
D004269:DNA, Bacterial