Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains.
PCR DNA fingerprinting using repetitive intergenic DNA sequences (rep-PCR) was investigated as a means of differentiating between closely related strains of Streptomyces which were, in some cases, indistinguishable by other classification methods. Our results demonstrated that the majority of strains had unique rep-PCR DNA fingerprints and established that the technique could be a very useful tool in rapidly determining strain identity.
Sadowsky
M J
MJ
Department of Soil, Water, and Climate, University of Minnesota, St. Paul 55108, USA.
Kinkel
L L
LL
Bowers
J H
JH
Schottel
J L
JL
D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015203:Reproducibility of Results; D013302:Streptomyces / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial