title: Simplified quantification of regional cerebral blood flow with 99mTc-ECD SPECT and continuous arterial blood sampling.
abstract: The goal of this study was to develop a simple method for quantification of regional cerebral blood flow (rCBF) with 99mTc-ethyl cysteinate dimer (ECD) SPECT. Following an intravenous constant infusion of ECD for one minute, serial dynamic SPECT imaging was performed for 40 minutes in 6 healthy male volunteers with intermittent arterial blood sampling. PET scan with 15O-water was performed on the same day before the SPECT study for measurement of rCBF. Arterial blood data demonstrated rapid conversion of ECD to the hydrophilic metabolites, and most of the arterial input to the brain was completed within 5 minutes after the injection. Brain activity reached a peak value soon after the cessation of infusion, and was stable thereafter with very little washout. Net extraction of ECD in the brain calculated by arterial input of ECD and rCBF demonstrated a rapid decrease within a few minutes, reaching 42.7% at 5 minutes. The simulation study suggested that the arterial blood activity obtained by continuous drawing for 5 minutes and a single SPECT scan would provide a reasonable estimate of rCBF under the assumption of constant net extraction in the brain.
authors.affiliation: Biomedical Imaging Research Center, Fukui Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yonekura
authors.forename: K
authors.initials: K
authors.lastname: Ishizu
authors.forename: H
authors.initials: H
authors.lastname: Okazawa
authors.forename: F
authors.initials: F
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Hattori
authors.forename: N
authors.initials: N
authors.lastname: Sadato
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.forename: S
authors.initials: S
authors.lastname: Nishizawa
authors.forename: N
authors.initials: N
authors.lastname: Tamaki
authors.forename: T
authors.initials: T
authors.lastname: Nagamine
authors.forename: J
authors.initials: J
authors.lastname: Konishi
authors.forename: H
authors.initials: H
authors.lastname: Shibasaki
mesh_terms: D000328:Adult; D001921:Brain / Q000098:blood supply / Q000000981:diagnostic imaging*; D002560:Cerebrovascular Circulation; D003545:Cysteine / Q000031:analogs & derivatives*; D006801:Humans; D008297:Male; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D012016:Reference Values; D012039:Regional Blood Flow; D013997:Time Factors; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D015609:Organotechnetium Compounds; C059659:technetium Tc 99m bicisate; D003545:Cysteine