title: Mucin-like glycoprotein genes are closely linked to members of the trans-sialidase super-family at multiple sites in the Trypanosoma cruzi genome.
abstract: In Trypanosoma cruzi a cell surface enzyme with trans-sialidase (TS) activity has been implicated as an important factor in establishing infection. The enzyme is encoded by genes belonging to a large super-family which on the basis of sequence has been subdivided into 4 groups. TS mediates the transfer of sialic acid residues from host glycoconjugates to acceptor molecules on the parasite surface. To study the organisation of the TS genes we isolated several distinct cosmids from a library constructed with DNA from the T. cruzi X10.6 clone. In these cosmids, the TS genes (group I) were present either as single copies or as a direct tandem repeat. A common feature of the cosmids was the presence of a related group III gene located 10-12kb downstream of the TS gene(s) and arranged in the same orientation. In several of the cosmids we also identified a mucin-like glycoprotein gene located between the group I and group III genes. The mucin-like genes are part of a large polymorphic family and contour clamped homogeneous electric field electrophoresis (CHEFE) analysis showed that they were linked to members of the TS super-family at multiple sites in the X10.6 genome. Screening of a second cosmid library made with DNA from the CL-Brener clone confirmed this multiple linkage suggesting that it is a common feature of the species. This genetic organisation may have important functional significance since the mucin-like glycoproteins are the major cell surface acceptors of sialic acid.
authors.affiliation: Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
authors.forename: N A
authors.initials: NA
authors.lastname: Salazar
authors.forename: A
authors.initials: A
authors.lastname: Mondragon
authors.forename: J M
authors.initials: JM
authors.lastname: Kelly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D017125:Genes, Protozoan; D008040:Genetic Linkage; D018503:Genome, Protozoan; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D006023:Glycoproteins; D009077:Mucins; D015800:Protozoan Proteins; C069715:trans-sialidase; D009439:Neuraminidase