Schizosaccharomyces pombe produces novel pyruvate-containing N-linked oligosaccharides.
The large N-linked oligosaccharides released by endo-beta-N-acetylglucosaminidase H from Schizosaccharomyces pombe glycoproteins were analyzed for the presence of noncarbohydrate functional groups. No phosphate, sulfate, or acetate could be detected; however, approximately six molecules of pyruvic acid/molecule were found on 98% of the oligosaccharides. Pyruvate moieties were acetal (ketal)-linked to galactose residues in the R configuration to carbons 4 and 6. This is the first report of pyruvate functional groups being attached to N-linked oligosaccharides in yeast and appears only to be the second documentation of this sugar modification in eukaryotes.
Gemmill
T R
TR
Department of Biomedical Sciences, State University of New York at Albany School of Public Health, Albany, New York 12201, USA.
Trimble
R B
RB
D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D006596:Hexosaminidases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D011773:Pyruvates / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; D011773:Pyruvates; D006596:Hexosaminidases