Delayed vasodilation and altered oxygenation after cerebral ischemia in fetal sheep.
The study investigated the hypothesis that delayed cerebral injury after transient cerebral ischemia is associated with vasoconstriction and decreased cerebral oxygenation. Eight chronically instrumented, late gestation fetal sheep were subjected to 30 min of cerebral ischemia in utero. Cortical impedance (CI) and electrocorticogram (ECoG) were recorded to determine the time course of cellular dysfunction. Histologic outcome was assessed 4 d postischemia. Changes in cerebral vascular tone and oxygenation were observed during and for 4 d after the insult using near infrared spectroscopy to measure changes in total cerebral Hb ([tHb]), oxyhemoglobin ([Hbo2]), and oxidized cytochrome aa3 ([Cyto2]). Results are expressed as mean +/- SEM. CI increased transiently during ischemia; then a delayed increase commenced 17.5 +/- 2.3 h postischemia and peaked at 42.3 +/- 2.4 h. ECoG was depressed during and after the insult. Seizures started 13.6 +/- 3.0 h postinsult and persisted for 25.4 +/- 3.2 h. Increases in [tHb] indicated two periods of cerebral vasodilation: immediately after early reperfusion, lasting 2.3 +/- 0.4 h and peaking to 20 +/- 2.0 mumol.L-1; and a later phase, commencing 12.8 +/- 2.0 h postischemia, peaking to 43 +/- 4.0 mumol.L-1 and lasting 43.1 +/- 5.2 h. [Hbo2] was relatively elevated (18 +/- 3.0 mumol.L-1) during d 4 postischemia, demonstrating a delayed increase in mean cerebral oxygen saturation. [Cyto2] fell during the insult (-0.7 +/- 0.2 mumol.L-1); and, commencing at 28-30 h postischemia, fell progressively to reach a minimum of -5.0 +/- 2.8 mumol.L-1 at 78-80 h postischemia. A greater fall in [Cyto2] was related to worse cerebral injury (p < 0.05). Delayed cerebral injury is accompanied by vasodilation and increased mean cerebral oxygen saturation, although a progressive fall in [Cyto2] might indicate a fall in mitochondrial oxygenation, cell loss, or changes in tissue optical characteristics.
Marks
K A
KA
Department of Paediatrics and Neonatal Medicine, Royal Postgraduate Medical School, London, United Kingdom.
Mallard
E C
EC
Roberts
I
I
Williams
C E
CE
Sirimanne
E S
ES
Johnston
B
B
Gluckman
P D
PD
Edwards
A D
AD
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D002339:Carotid Arteries; D004195:Disease Models, Animal; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005319:Fetal Hemoglobin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D010100:Oxygen; D010108:Oxyhemoglobins / Q000378:metabolism; D012756:Sheep; D014664:Vasodilation / Q000502:physiology*
D006454:Hemoglobins; D010108:Oxyhemoglobins; D005319:Fetal Hemoglobin; D003576:Electron Transport Complex IV; D010100:Oxygen