A perfluorochemical prevents ischemia-reperfusion injury of muscle.
This investigation evaluated the effect of oxygenated perfluorochemical (PFC) perfusion on the viability of ischemic skeletal muscle compared to hypothermic preservation. Twenty-five hindlimbs of 13 white rabbits were divided into five groups: a PFC group (7 hr of ischemia with 6 hr of PFC perfusion), a PFC/ reperfusion group (8 hr of ischemia with 6 hr of PFC perfusion and 1 hr reperfusion), a hypothermia group (7 hr of ischemia with 6 hr of 4 degrees C cold preservation), a hypothermia/reperfusion group (8 hr of ischemia with 6 hr of 4 degrees C cold preservation and 1 hr of reperfusion), and a control group. The levels of adenine nucleotides, creatine phosphate, hypoxanthine, xanthine, and lipid peroxide were determined in each group. In the PFC and PFC/reperfusion groups, the ATP level remained at 90% of that in the control group. The hypoxanthine level in the hypothermia/reperfusion group was decreased to 70% of that in the hypothermia group and the xanthine level was increased to 130%. In the PFC and PFC/reperfusion groups, hypoxanthine and xanthine levels were much lower and were similar to those in the control group. Lipid peroxide levels were also lower in the PFC and PFC/reperfusion groups than in the hypothermia/reperfusion group. Electron microscopy showed that endothelial cells from the PFC/reperfusion groups were not swollen. These results suggest that PFC perfusion is superior to hypothermia in inhibiting the generation of free radicals and in preventing ischemia-reperfusion injury of skeletal muscle.
Yabe
Y
Y
Department of Orthopedic Surgery, Nagoya University School of Medicine, Japan.
Ishiguro
N
N
Shimizu
T
T
Kawasaki
S
S
Sasaki
Y
Y
Iwata
H
H
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004734:Energy Metabolism; D005466:Fluorocarbons / Q000494:pharmacology*; D006614:Hindlimb; D007511:Ischemia; D008054:Lipid Peroxides / Q000096:biosynthesis; D008854:Microscopy, Electron; D009132:Muscles / Q000098:blood supply* / Q000378:metabolism; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control*; D012092:Replantation; D019820:Xanthine; D014970:Xanthines / Q000378:metabolism
D000227:Adenine Nucleotides; D005466:Fluorocarbons; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D014970:Xanthines; D010725:Phosphocreatine; D019820:Xanthine; C008807:perfluorotributylamine