Comparison of plasmid DNA preparation methods for direct gene transfer and genetic immunization.
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.
Davis
H L
HL
Loeb Medical Research Institute, Ottawa Civic Hospital, ON, Canada. hdavis@civich.ottawa.on.ca
Schleef
M
M
Moritz
P
P
Mancini
M
M
Schorr
J
J
Whalen
R G
RG
D000818:Animals; D000838:Anions; D002586:Cesium; D002712:Chlorides; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D007114:Immunization; D008156:Luciferases / Q000235:genetics; D051379:Mice; D018482:Muscle, Skeletal; D010957:Plasmids / Q000235:genetics*; D019444:Vaccines, DNA / Q000276:immunology*
D000838:Anions; D002712:Chlorides; D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens; D019444:Vaccines, DNA; D002586:Cesium; D004247:DNA; D008156:Luciferases; C028019:cesium chloride