Complement activation by IgG immobilized on methylated silicon.
Activation of the complement system by immobilized IgG on methylated silicon was studied by ellipsometry/antibody-, ELISA-, and RIA techniques after exposure to human serum at 37 degrees C for up to 1 h. The IgG-covered surfaces rapidly activated the complement system and the combined results suggest an initial classical pathway activation. Complement factor 1q (C1q) and IgG were antibody-detectable on the surfaces for serum incubations up to 5 min but not thereafter. Anti-C3c and anti-properdin bound to the surfaces at all serum incubation times. Experiments with 125I-IgG preadsorbed to surfaces, or added to normal-, EGTA-, and EDTA-sera, showed that IgG was not displaced from the protein film by serum.
Tengvall
P
P
Laboratory of Applied Physics, Linköping University, Sweden.
Askendal
A
A
Lundström
I
I
D015704:CD4 Antigens / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003167:Complement Activation / Q000187:drug effects*; D015932:Complement C3c / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D008745:Methylation; D011863:Radioimmunoassay; D017655:Silicon Compounds / Q000737:chemistry / Q000276:immunology*; D013499:Surface Properties
D015704:CD4 Antigens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D017655:Silicon Compounds; D015932:Complement C3c