North American Multicenter Study on flow cytometric enumeration of CD34+ hematopoietic stem cells.
Transplant centers often rely on CD34+ cell quantitation by flow cytometry to ensure adequacy of hematopoietic progenitor cell collection. Because of variation in interpretation, a lack of interlaboratory proficiency studies, and no generally accepted methodology, comparison of CD34 data from site to site is difficult. Twenty-one samples from marrow and peripheral blood stem cell collections were shipped to 10 participating North American laboratories for analysis. Duplicate samples were included to assess reproducibility. Participants were surveyed for methodology. Three centers had previously attempted to standardize their methodology among themselves. The variability observed in the CD34 values ranged from a max/min. reported value per sample of 2.9 to 749 (median 76). Exclusion of two outlying sites reduced the variability of results to 1.2 to 27 (median 3.1). Variation among the three standardized sites ranged from 1.2 to 4.4 (median 1.6). Overall reproducibility (excluding the outlying sites B and G) ranged from a minimum of 0-16.5 (percent mean difference) for site C to a maximum of 4.1-133 for site H. Strategies for gating were found to largely influence results. We observed an alarming variation among the CD34 cell counts reported from different laboratories. Standardization substantially reduced observed variation. The need for standardized methodology, reporting, quality control, and proficiency testing is underscored by these findings.
Brecher
M E
ME
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill 27514, USA.
Sims
L
L
Schmitz
J
J
Shea
T
T
Bentley
S A
SA
D018952:Antigens, CD34 / Q000032:analysis*; D005434:Flow Cytometry / Q000592:standards; D006405:Hematology / Q000592:standards; D018380:Hematopoietic Stem Cell Transplantation / Q000592:standards*; D006801:Humans; D007753:Laboratories / Q000592:standards*; D015203:Reproducibility of Results; D013234:Stem Cells / Q000276:immunology*; D014481:United States
D018952:Antigens, CD34