title: Engineering new functions and altering existing functions.
abstract: Structural and mechanistic information, sequence comparisons, and site-directed mutagenesis data continue to provide a basis for the rational design of new protein functions and the alteration of existing functions. Random mutagenesis and 'directed evolution' approaches, however, are making significant headway in solving protein engineering problems, proving highly practical for tuning properties such as enzyme substrate specificity.
authors.affiliation: Division of Chemistry and Chemical Engineering, 210-41, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Shao
authors.forename: F H
authors.initials: FH
authors.lastname: Arnold
mesh_terms: D001665:Binding Sites; D019020:Directed Molecular Evolution; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins