title: New compatible solutes related to Di-myo-inositol-phosphate in members of the order Thermotogales.
abstract: The accumulation of intracellular organic solutes was examined in six species of the order Thermotogales by nuclear magnetic resonance spectroscopy. The newly discovered compounds di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate and di-myo-inositol-1,3'-phosphate were identified in Thermotoga maritima and Thermotoga neapolitana. In the latter species, at the optimum temperature and salinity the organic solute pool was composed of di-myo-inositol-1,1'(3,3')-phosphate, beta-glutamate, and alpha-glutamate in addition to di-myo-inositol-1,3'-phosphate and di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate. The concentrations of the last two solutes increased dramatically at supraoptimal growth temperatures, whereas beta-glutamate increased mainly in response to a salinity stress. Nevertheless, di-myo-inositol-1,1'(3,3')-phosphate was the major compatible solute at salinities above the optimum for growth. The amino acids alpha-glutamate and proline were identified under optimum growth conditions in Thermosipho africanus, and beta-mannosylglycerate, trehalose, and glycine betaine were detected in Petrotoga miotherma. Organic solutes were not detected, under optimum growth conditions, in Thermotoga thermarum and Fervidobacterium islandicum, which have a low salt requirement or none.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal.
authors.forename: L O
authors.initials: LO
authors.lastname: Martins
authors.forename: L S
authors.initials: LS
authors.lastname: Carreto
authors.forename: M S
authors.initials: MS
authors.lastname: Da Costa
authors.forename: H
authors.initials: H
authors.lastname: Santos
mesh_terms: D006089:Gram-Negative Anaerobic Bacteria / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D007295:Inositol Phosphates / Q000737:chemistry*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008362:Mannosides / Q000737:chemistry; D008958:Models, Molecular; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D013696:Temperature
chemical_list: D007295:Inositol Phosphates; D008362:Mannosides; C101897:di-2-O-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate; D012965:Sodium Chloride