Production of IL-5 and IL-6 by peripheral blood mononuclear cells (PBMC) from patients with Echinococcus granulosus infection.
The role of Th2 cytokines in human hydatidosis was evaluated in ELISA determining IL-5 and IL-6 production in PBMC cultures from 27 pharmacologically treated hydatid patients and from 13 uninfected controls. PBMC from patients produced large amounts of parasite antigen-driven IL-5, whereas PBMC from uninfected individuals produced none. In contrast, PBMC from patients and from uninfected controls produced large amounts of parasite antigen-driven IL-6. Immunoglobulin isotype analysis revealed that IL-5 production correlated significantly with IgE and IgG4 expression (IL-5/IgE r = 0.5, P < 0.05; IL-5/IgG4 r = 0.6, P < 0.05). The high IL-5 levels in supernatants from patients' PBMC did not correspond to an increase in eosinophils. Neither IL-5 nor IL-6 production showed an association with the outcome of therapy. Overall, these findings confirm that the lymphocytes of individuals with Echinococcus granulosus infection contain Th2-like subpopulations.
Riganò
R
R
Laboratorio di Immunologia, Universita La Sapienza, Rome, Italy.
Profumo
E
E
Teggi
A
A
Siracusano
A
A
D015766:Albendazole / Q000627:therapeutic use; D000818:Animals; D004443:Echinococcosis / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D004446:Echinococcus / Q000472:pathogenicity; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000469:parasitology; D008297:Male; D018418:Th2 Cells / Q000378:metabolism
D007132:Immunoglobulin Isotypes; D015848:Interleukin-5; D015850:Interleukin-6; D015766:Albendazole