Replication and expression of a recombinant Sindbis virus in mosquitoes.
A recombinant Sindbis virus, TE/3'2J/ANTI-S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated into Aedes triseriatus mosquitoes. Virus replication and LAC-ANTI-S RNA expression were analysed temporally and spatially. TE/3'2J/ANTI-S virus titre peaked at 5.0 log10 TCID50 in heads 6-9 days post infection (p.i.) and decreased to 3.4 log10 TCID50 by 37 days p.i. Salivary glands contained 4.4 log10 TCID50 of virus 6 days p.i.; titres were lower in other organs. LAC-ANTI-S RNA levels paralleled virus titre. SIN E1 antigen was detected in many mosquito organ systems, but in specific cells and tissues of some organs. TE/3'2J/ANTI-S virus exhibited different cellular tropisms in salivary glands of Aedes and Culex mosquitoes.
Rayms-Keller
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Department of Microbiology, Colorado State University, Fort Collins, USA.
Powers
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Higgs
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Olson
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Kamrud
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Carlson
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Beaty
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BJ
D000330:Aedes / Q000378:metabolism / Q000821:virology*; D000818:Animals; D002460:Cell Line; D003465:Culex / Q000378:metabolism / Q000821:virology*; D015870:Gene Expression / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*; D012469:Salivary Glands; D012845:Sindbis Virus / Q000235:genetics* / Q000502:physiology; D014764:Viral Proteins; D014779:Virus Replication / Q000235:genetics*
D008562:Membrane Glycoproteins; D011498:Protein Precursors; D014764:Viral Proteins; C102373:glycoprotein PE2, Sindbis virus