title: Primary cultivation of human synovial cells from nonrheumatic synovial tissue and fluid.
abstract: Cultures were established from adherent cells of synovial fluid and from collagenase-dispersed specimens of synovial tissue. In cultures derived from synovial tissue, prolyl hydroxylase-positive cells of fibroblast-like morphology were identified as the predominant cell type. In cultures from synovial fluid the majority of adherent cells was macrophage-like in appearance and strongly positive for CD 68. Cells with a stellate morphology could rarely be observed in cultures from synovial tissue. Their relations to other forms will be discussed. Several simple methods for cultivating adherent synovial cells are presented.
authors.affiliation: Institut für Medizinische Mikrobiologie, Friedrich-Schiller-Universität Jena, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Rödel
authors.forename: E
authors.initials: E
authors.lastname: Straube
authors.forename: W
authors.initials: W
authors.lastname: Lungershausen
authors.forename: A
authors.initials: A
authors.lastname: Roth
authors.forename: A
authors.initials: A
authors.lastname: Groh
mesh_terms: D018929:Cell Culture Techniques; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007596:Joints / Q000166:cytology*; D013582:Synovial Fluid / Q000166:cytology*; D013583:Synovial Membrane / Q000166:cytology*