Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation.
The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIs). In vitro experiments have suggested that TAFIIs are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFII, yeast TAFII90 (yTAFII90). We show that functional inactivation of yTAFII90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAFII90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAFII90 is not required for transcription activation in general, and reveal linkages between TAF function and cell-cycle progression.
Apone
L M
LM
Howard Hughes Medical Institute, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA.
Virbasius
C M
CM
Reese
J C
JC
Green
M R
MR
D000483:Alleles; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D016195:G2 Phase / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012319:RNA Polymerase II / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000235:genetics; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D013696:Temperature; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015003:Yeasts / Q000235:genetics* / Q000502:physiology; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012319:RNA Polymerase II; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C087176:TAF2 protein, S cerevisiae