Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor.
We established a monoclonal antibody against a surface antigen of activated macrophages (Møs) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Møs also expressed the antigen but resident or inflammatory Møs, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Møs exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Møs. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Møs.
Takikawa
O
O
Department of Cell Biology, Osaka Bioscience Institute, Japan.
Oku
T
T
Ito
N
N
Ushio
Y
Y
Yamamoto
N
N
Yoneda
Y
Y
Tsuji
J
J
Sanchez-Bueno
A
A
Verkhusha
V
V
Yoshida
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D015152:Blotting, Northern; D002460:Cell Line; D018076:DNA, Complementary; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology*; D008262:Macrophage Activation / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation / Q000276:immunology*; D012322:RNA Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D000954:Antigens, Surface; D018076:DNA, Complementary; D012322:RNA Precursors; D012333:RNA, Messenger