Resolution improvement by use of carboxymethyl-beta-cyclodextrin as chiral additive for the enantiomeric separation of basic drugs by capillary electrophoresis.
Three beta-cyclodextrin derivatives--carboxymethyl-, dimethyl- and hydroxypropyl-beta-cyclodextrin--were tested as chiral selectors for the enantioseparation of seven basic drugs in free solution capillary electrophoresis, using buffers made of 100 mM phosphoric acid adjusted to pH 3.0 with triethanolamine in fused silica capillaries thermostatted at 15 degrees C. The best results with respect to chiral resolution were obtained with carboxymethyl-beta-cyclodextrin (CMCD): the enantiomers of all compounds examined were completely resolved with this beta-cyclodextrin derivative. The influence of the CMCD concentration on the migration times, the apparent electrophoretic mobility difference and the resolution of the drug enantiomers was investigated thoroughly. Particularly impressive resolution values, up to 23.7, were obtained for several compounds in these capillary electrophoretic systems, using CMCD in the 5-15 mM concentration range.
Fillet
M
M
Laboratory of Drug Analysis, Institute of Pharmacy, University of Liège, Belgium.
Bechet
I
I
Hubert
P
P
Crommen
J
J
D000779:Anesthetics, Local / Q000302:isolation & purification; D001993:Bronchodilator Agents / Q000302:isolation & purification; D002045:Bupivacaine / Q000302:isolation & purification; D002744:Chlorpheniramine / Q000302:isolation & purification; D003505:Cyclodextrins; D004115:Dimethindene / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D004809:Ephedrine / Q000302:isolation & purification; D005277:Fenfluramine / Q000302:isolation & purification; D006634:Histamine H1 Antagonists / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007545:Isoproterenol / Q000302:isolation & purification; D009994:Osmolar Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D018490:Serotonin Agents / Q000302:isolation & purification; D013237:Stereoisomerism; D013726:Terbutaline / Q000302:isolation & purification
D000779:Anesthetics, Local; D001993:Bronchodilator Agents; D003505:Cyclodextrins; D006634:Histamine H1 Antagonists; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D018490:Serotonin Agents; D005277:Fenfluramine; D002744:Chlorpheniramine; D004115:Dimethindene; D004809:Ephedrine; D007545:Isoproterenol; D013726:Terbutaline; D002045:Bupivacaine