Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes.
New aqueous liquid-liquid two-phase systems based on bovine serum albumin and sodium thiocyanate in combination with either poly(vinyl alcohol) or poly(ethylene glycol) were investigated. Phase diagrams are presented. Lactate dehydrogenase and some mitochondrial enzymes were partitioned in the systems. All the phase components used influenced, either positively or negatively, the activity of lactate dehydrogenase. The enzymes showed a strong preference for the albumin phase. Possible scientific and biotechnological uses are discussed.
Johansson
G
G
Department of Biochemistry, University of Lund, Sweden.
D000818:Animals; D002021:Buffers; D002417:Cattle; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry*; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002021:Buffers; D004798:Enzymes; D010710:Phosphates; D011108:Polymers; D012997:Solvents; D013861:Thiocyanates; D014867:Water; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C024553:sodium thiocyanate; D011142:Polyvinyl Alcohol; D007770:L-Lactate Dehydrogenase