Comparison of supercritical CHF3 and CO2 and methanol-modified CHF3 and CO2 for extraction of sulfonamides from chicken liver.
Supercritical CHF3 and methanol-modified CHF3 were compared with supercritical CO2 and methanol-modified CO2 for extraction of sulfonamides from fortified chicken liver admixed with Hydromatrix. Results showed that solvating power and selectivity were higher for supercritical and methanol-modified CHF3 than for supercritical and methanol-modified CO2. Visual observation showed that chicken liver extract obtained with methanol-modified CHF3 was cleaner than that obtained with methanol-modified CO2. Fat precipitated in the solvent trap when CO2 was used as the extraction medium. Also, simple off-line collection of fortified chicken liver extract obtained with CO2 in a solid-phase extraction cartridge (packed with either C18 or alumina) followed by phosphate buffer-methanol (50 + 50) rinse yielded an extract that required no further cleanup for analysis.
Ashraf-Khorassani
M
M
Virginia Polytechnic Institute and State University, Department of Chemistry, Blacksburg 24061, USA.
Taylor
L T
LT
Schweighardt
F K
FK
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002245:Carbon Dioxide / Q000737:chemistry*; D002645:Chickens; D005617:Chlorofluorocarbons, Methane / Q000737:chemistry*; D002853:Chromatography, Liquid; D008099:Liver / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D012015:Reference Standards; D012995:Solubility; D013412:Sulfadimethoxine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013418:Sulfamethazine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013428:Sulfaquinoxaline / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013449:Sulfonamides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014280:Triglycerides / Q000737:chemistry
D000890:Anti-Infective Agents; D005617:Chlorofluorocarbons, Methane; D013449:Sulfonamides; D014280:Triglycerides; D002245:Carbon Dioxide; D013412:Sulfadimethoxine; D013418:Sulfamethazine; D013428:Sulfaquinoxaline; D000432:Methanol; C009554:fluoroform