Cloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94.
A gene which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I of homology) and total resistance to the small isometric-headed bacteriophage phi 59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.
Prévots
F
F
Systems Bio-Industries, Boulogne Billancourt, France.
Daloyau
M
M
Bonin
O
O
Dumont
X
X
Tolou
S
S
D001426:Bacterial Proteins / Q000235:genetics*; D001435:Bacteriophages / Q000472:pathogenicity*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D007113:Immunity, Innate / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics* / Q000821:virology*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins