Assay of different photoproducts after UVA, B and C irradiation of DNA and human skin explants.
A 32P-HPLC method was applied to study the induction of UVB- and UVC-induced DNA lesions (cyclobutane dimers, 6-4 photoproducts and Dewar isomers) in human skin explants. The employed technique was sensitive enough to detect the lesions at a dose of 10 J/m2 UVB. Comparison of photoadduct formation under UVC and UVB indicated the importance of photosensitization pathways in DNA damage. Dewar isomers were detected only at a high dose of UVB. The compounds were identified by their photochemical reactivity and by spiking with prepared standards. Treatment with nuclease P1 was used to identify the 5'-terminal nucleotide. UVA caused no detectable adducts.
Bykov
V J
VJ
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Hemminki
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005260:Female; D006801:Humans; D009924:Organ Culture Techniques; D010766:Phosphorylation; D011740:Pyrimidine Dimers / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D000255:Adenosine Triphosphate; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases