Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.
A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
Hassan-King
M
M
Medical Research Council Laboratories, Fajara, The Gambia, West Africa.
Baldeh
I
I
Adegbola
R
R
Omosigho
C
C
Usen
S O
SO
Oparaugo
A
A
Greenwood
B M
BM
D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D003470:Culture Media; D005714:Gambia / Q000453:epidemiology; D006192:Haemophilus Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D011008:Pneumococcal Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D018410:Pneumonia, Bacterial / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D003470:Culture Media