Evidence that the Bacillus subtilis pyrimidine regulatory protein PyrR acts by binding to pyr mRNA at three sites in vivo.
The Bacillus subtilis pyr operon is regulated by a transcriptional attenuation mechanism that requires the PyrR regulatory protein. Multicopy plasmids that could be transcribed to yield segments of RNA from the attenuation regions of the pyr operon induced derepression of chromosomal pyr genes, whereas plasmids that could not yield pyr RNA did not. We conclude that pyr RNA acts by titrating the PyrR protein and preventing it from regulating pyr attenuation.
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Department of Biochemistry, University of Illinois, Urbana 61801, USA.
Switzer
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D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D010430:Pentosyltransferases / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D011742:Pyrimidine Nucleotides / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D011742:Pyrimidine Nucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; D010430:Pentosyltransferases; C098851:PyrR protein, bacteria