title: Water molecule binding and lifetimes on the DNA duplex d(CGCGAATTCGCG)2.
abstract: Measurements of the water proton spin-lattice relaxation rate for aqueous solutions of the palindromic dodecamer, d(CGCGAATTCGCG)2, are reported as a function of the magnetic field strength. The magnitude of the relaxation rates at low magnetic field strengths and the shape of the relaxation dispersion curve permit assessment of the number of water molecules which may be considered bound to the DNA for a time equal to or longer than the rotational correlation time of the duplex. The data are examined using limiting models that arbitrarily use the measured rotational correlation time of the polynucleotide complex as a reference point for the water molecule lifetime. If it is assumed that water molecules are bound at DNA sites for times as long as or longer than the rotational correlation time of the duplex, then the magnitude of the relaxation rates at low field require that there may be only two or three such water sites. However, if the lifetime constraint is relaxed, and we assume that the number of water molecules bound to the DNA is more nearly the number identified in the X-ray structures, then the average water molecule lifetime is on the order of 1 ns. Measurements of 1H NOESY spectra demonstrate that some water molecules must have lifetimes sufficiently long that negative Overhauser effects are observed. Taken together, these results suggest a distribution of water molecule lifetimes in which most of the DNA-bound water molecule lifetimes are shorter than the rotational correlation time of the duplex, but where some have lifetimes of at least 1 ns under these concentrated conditions.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville, 22901, USA.
authors.forename: D
authors.initials: D
authors.lastname: Zhou
authors.forename: R G
authors.initials: RG
authors.lastname: Bryant
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D017422:Sequence Analysis, DNA; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D004247:DNA