title: A comparison of the nucleotide sequence of the cerato-ulmin gene and the rDNA ITS between aggressive and non-aggressive isolates of Ophiostoma ulmi sensu lato, the causal agent of Dutch elm disease.
abstract: Little genetic information exists comparing aggressive and non-aggressive isolates of the causal agent of Dutch elm disease, Ophiostoma ulmi. Two genetic elements were compared between the subgroups. The ceratoulmin cu gene product has been associated with disease symptoms. Nucleotide-sequence analysis of cu and the internal transcribed spacer (ITS) region of the rDNA were made from three aggressive and three non-aggressive isolates of the pathogen. Our results suggested uniformity within, and unique differences between, subgroups. Differences were detected for cu in the promoter, coding, and transcription termination regions. Sequence data for the ITS clearly distinguish the subgroups.
authors.affiliation: Faculty of Forestry, University of Toronto, Ontario, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Jeng
authors.forename: W E
authors.initials: WE
authors.lastname: Hintz
authors.forename: C G
authors.initials: CG
authors.lastname: Bowden
authors.forename: P A
authors.initials: PA
authors.lastname: Horgen
authors.forename: M
authors.initials: M
authors.lastname: Hubbes
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009183:Mycotoxins; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal; D005656:Fungal Proteins; D009183:Mycotoxins; C079060:CU protein, Ophiostoma ulmi