title: Crystallization and preliminary X-ray analysis of salicylate hydroxylase from Pseudomonas putida S-1.
abstract: Apo-salicylate hydroxylase from Pseudomonas putida S-1 has been crystallized by the dialysis method, using ammonium sulfate as the precipitant. The crystals belong to hexagonal space group P6(2) or P6(4) with unit cell dimensions of a = b = 142.8 A and c = 63.8 A, and diffract X-rays at higher than 3.5 A resolution. A heavy-atom derivative has been prepared by soaking a crystal in an ammonium sulfate solution containing p-chloromercuriphenylsulfonate.
authors.affiliation: Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima.
authors.forename: T
authors.initials: T
authors.lastname: Yabuuchi
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Ohnishi
authors.forename: E
authors.initials: E
authors.lastname: Itagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000302:isolation & purification; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase