Autologous mixed leucocyte reaction and the polyclonal activation of bovine gamma/delta T cells.
Bovine gamma/delta T cells proliferate in response to stimulation with gamma-irradiated autologous monocytes in the autologous mixed leukocyte reaction (AMLR). Flow cytometric analyses indicated that the proliferating cells included three major subpopulations of bovine gamma/delta T cells, distinguished by the differential expression of the gamma/delta T cell receptor epitopes N6 and N7. Interleukin-2 and acid-labile interferon were produced in AMLR cultures but the cultured cells did not lyse any of a large variety of target cells, including monocytes, allogeneic lymphoblasts, transformed bovine B cells (BL3), bovine fibroblast and the natural killer cell targets D17 and K562, even in the presence of lectins or after co-stimulation in the AMLR with antibodies to WC1, the gamma/delta T cell lineage-specific cell-surface differentiation antigen. Ex vivo gamma/delta T cells did not display lymphokine-activated killing whereas populations of peripheral blood mononuclear cells containing alpha/beta T cells did.
Hanby-Flarida
M D
MD
Department of Microbiology, Ohio State University, Columbus 43210-1292, USA.
Okragly
A J
AJ
Baldwin
C L
CL
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005720:Gamma Rays; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D007700:Kinetics; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000276:immunology* / Q000528:radiation effects; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins