Functional conservation of the Salmonella and Shigella effectors of entry into epithelial cells.
A Salmonella typhi chromosomal locus composed of five adjacent genes, designated sipEBCDA, was identified by transposon mutagenesis as being essential for cell invasion. Products of the sip genes exhibit extensive sequence similarities to the effectors of Shigella entry into epithelial cells encoded by the virulence plasmid-borne ipa operon. Expression of sipE and sipB in a Shigella non-invasive ipaB mutant restored the ability to invade epithelial cells. The structural and functional conservation of the Sip and Ipa proteins suggests that Salmonella and Shigella entry processes are promoted by similar effectors.
Hermant
D
D
Unité des Entérobactéries et Unité INSERM 389, Institut Pasteur, Paris, France.
Ménard
R
R
Arricau
N
N
Parsot
C
C
Popoff
M Y
MY
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D017124:Conserved Sequence; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012485:Salmonella typhi / Q000235:genetics* / Q000472:pathogenicity*; D017386:Sequence Homology, Amino Acid; D012760:Shigella / Q000235:genetics; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D008565:Membrane Proteins; C094579:invasion protein B, Salmonella typhimurium; C087447:ipaB protein, Shigella