title: Cell membrane permeability and mitochondrial dysfunction-inducing activities in cell-free supernatants from Serpulina hyodysenteriae serotypes 1 and 2.
abstract: Membrane permeability (MP) and mitochondrial dysfunction-inducing (MDI) activities were detected in cell-free supernatants (CFS) of Serpulina hyodysenteriae, using either hemoglobin release from porcine red blood cells (RBC) or cytoplasmic lactate dehydrogenase release from porcine peripheral blood lymphocytes (PBL), and reduction of the 3-(4,5-dimethylthiazoyl-2-y1)-2,5-diphenyltetrazolium bromide dye by porcine PBL. The MP and MDI activities of CFS correlated with each other for serotype 1 and 2 isolates taken at different population densities; however, the kinetics of toxin production varied between each serotype. The loss of enteropathogenicity of two field isolates with nonpathogenic phenotypes and pathogenic isolates passaged up to 45 times in vitro was not attributable to a loss of either membrane permeability or mitochondrial dysfunction-inducing activity of cell-free supernatants. Results from this study suggested the potential for two separate toxins being involved in the pathogenesis of swine dysentery, with the MDI activity correlating with age susceptibility to clinical disease.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln 68583-0905, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Witters
authors.forename: G E
authors.initials: GE
authors.lastname: Duhamel
mesh_terms: D000367:Age Factors; D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000145:classification / Q000472:pathogenicity* / Q000502:physiology*; D002463:Cell Membrane Permeability; D003470:Culture Media / Q000737:chemistry; D004403:Dysentery / Q000382:microbiology / Q000662:veterinary*; D006460:Hemolysin Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000502:physiology*; D013145:Spirochaetales Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D003470:Culture Media; D006460:Hemolysin Proteins