Induction of glomerulonephritis in rats with staphylococcal phosphatase: new aspects in post-infectious ICGN.
Staphylococcal neutral phosphatase (NPtase) is a highly cationic bacterial surface bound protein. It has significant affinity for human and rat immunoglobulins in vitro and an electrostatic interaction may be involved. Radioisotopic studies showed that NPtase had a high affinity for the polyanionic structures of the rat renal glomerulus. When the left kidneys of germ-free or naive (non-immune) Wistar rats were perfused with 80 micrograms of I125 NPtase, 21 micrograms of NPtase were found in the left kidneys and 11 micrograms in the isolated glomeruli 15 minutes after perfusion. Deposits of autologous immunoglobulin and C3 were seen in the glomeruli of rats immediately after perfusion with NPtase (15 min) and persisted throughout the 14-day observation period. Histologically, neutrophil influx into the glomerulus was seen at 15 minutes and increased until three hours; subepithelial electron-dense deposits were found after three days and were still visible on day 14. Proteinuria started within the first 24 hours despite the absence of an immune response at this time and was still present on day 14. Similar results were observed in immune deficient athymic nude rats in the early phase. Perfusion of heparin after NPtase inhibited the deposition of IgG and C3 and prevented proteinuria in naive but not in actively immunized rats. This result provides further evidence that specific antibodies to NPtase were not involved in the immune complex-like deposits seen in the early phase. NPtase is a novel molecule, as it reveals both high affinity for the GBM and binding of circulating immunoglobulins, by a non-antigen-antibody mechanism, to form IC-like deposits on the GBM. These deposits are capable of activating the complement system, thus triggering a series of events leading to glomerulonephritis. These results delineate an additional pathway for the pathogenesis of ICGN related to bacterial infection.
Yousif
Y
Y
Department of Immunology, Institute of Medical Microbiology, Freiburg University, Germany.
Okada
K
K
Batsford
S
S
Vogt
A
A
D000818:Animals; D003176:Complement C3 / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000209:etiology* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010744:Phosphoric Monoester Hydrolases / Q000493:pharmacokinetics / Q000633:toxicity*; D011507:Proteinuria / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000150:complications*
D003176:Complement C3; C092526:Staphylococcal neutral phosphatase; D010744:Phosphoric Monoester Hydrolases