A simple solid-phase method for the identification of red cell-bound antibodies without elution.
The authors have developed a solid-phase direct hemadherence assay (SPDHA) to identify red cell-bound antibody without elution. The procedure of SPDHA is as follows: (1) commercially available panel cells were immobilized on the well of microplane; (2) 22% polymerized albumin and 0.3% test red cells were added, and the plate was centrifuged at low speed, and incubated; (3) finally the plate was centrifuged, and the results were read macroscopically. SPDHA could detect antibodies against D, C, c, E, e, Fya, Fyb, K, k, A and B antigens. The sensitivity of SPDHA was high in Rh antibodies as compared with that in the other antibodies. SPDHA failed to detect anti-Jka, -Jkb, -S, -s and -Dia antibodies. In cases of suspected hemolytic disease of newborn, Rh antibodies could be identified using very small volume of red cells. In conclusion, SPDHA is a useful and simple method to identify red cell-bound antibodies, especially when only a small volume of red cell sample is available, such as the sample from fetus or newborn.
Nagamine
K
K
Department of Blood Transfusion, Osaka University Hospital, Japan.
Kiyokawa
T
T
Aochi
H
H
Oshida
M
M
Hayashi
S
S
Tomiyama
Y
Y
Kurata
Y
Y
D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D004899:Erythroblastosis, Fetal / Q000276:immunology*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn
D000906:Antibodies