title: Complex interaction between different proteinaceous components within the cell-wall structure of Candida albicans.
abstract: We have previously described a monoclonal antibody, MAb DC3:H10, which recognized an epitope preferentially expressed on the surface of Candida albicans germ tubes. In the present study we examined the MAb-reactive material further. Immunoblot analysis of the material purified partially by Sephadex G-200 and DEAE-Sephacel chromatography reacted with antibodies to the C. albicans C3d receptor (CR2). In an ELISA, MAb DC3:H10 captured antigen that was recognized by both anti-CR2 and anti-mp58 fibrinogen binding mannoprotein polyclonal antibodies. The MAb DC3:H10 failed to compete with either of these antisera in an ELISA. Indirect immunofluorescence (IIF) analysis showed differences in surface distribution for the MAb DC3:H10, the CR2, and the mp 58 epitopes. Dual labeling IIF experiments showed MAb DC3:H10 binding to be unaffected by binding of fibrinogen or anti-mp58 antibody. However, the binding patterns of MAb DC3:H10 were modified in the presence of anti-CR2 antibody, suggesting a complex interaction between these cell wall components.
authors.affiliation: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Lopez-Ribot
authors.forename: D A
authors.initials: DA
authors.lastname: Cortlandt
authors.forename: D C
authors.initials: DC
authors.lastname: Straus
authors.forename: K J
authors.initials: KJ
authors.lastname: Morrow
authors.forename: W L
authors.initials: WL
authors.lastname: Chaffin
mesh_terms: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000737:chemistry* / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D015151:Immunoblotting; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D017464:Receptors, Complement 3d / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D017464:Receptors, Complement 3d; C025363:mannoproteins