title: Comparison of reversed phase and reversed phase ion pair high performance liquid chromatography for analysis of TcO and TcN complexes of L,L-ethylene dicysteine di-ethylester and its acid analogues.
abstract: 99mTc(V)-oxo complexes of L,L-ethylene dicysteine (L,L-EC) and its di-ester derivative L,L-ethyl cysteinate dimer (L,L-ECD) are useful tracer agents for evaluation of renal function and cerebral blood flow respectively. Labelling of these molecules with a 99mTc(V)-nitrido core instead of the 99mTc(V)-oxo core alters their biological and physiochemical behavior. In the reversed phase high performance liquid chromatography (HPLC) method presently used to analyse 99mTc(V)-oxo preparations of L,L-EC and L,L-ECD, the 99mTc(V)-nitrido-L,L-EC complex and the possible impurities of a 99mTc(V)-nitrido-L,L-ECD preparation were found to elute with the void volume. In this study, a reversed phase ion pair HPLC method has been developed that is useful for the analysis of both 99mTc(V)-oxo and 99mTc(V)-nitrido preparations of L,L-EC and L,L-ECD. Tetrabutylammonium hydroxide is used as a cationic ion pairing agent.
authors.affiliation: Laboratory of Radiopharmaceutical Chemistry, K.U. Leuven, U.Z. Gasthuisberg, Belgium.
authors.forename: K O
authors.initials: KO
authors.lastname: Mang'era
authors.forename: E
authors.initials: E
authors.lastname: Bellande
authors.forename: R
authors.initials: R
authors.lastname: Pasqualini
authors.forename: A
authors.initials: A
authors.lastname: Verbruggen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis; D007553:Isotope Labeling; D015609:Organotechnetium Compounds / Q000032:analysis*; D011849:Radioactive Tracers; D013670:Sodium Pertechnetate Tc 99m / Q000032:analysis
chemical_list: D015609:Organotechnetium Compounds; D011849:Radioactive Tracers; C073806:technetium Tc 99m-ethylenedicysteine; D013670:Sodium Pertechnetate Tc 99m; D003545:Cysteine