Studies on the response of carrot cells to a Sclerotinia sclerotiorum elicitor: induction of the expression of an extracellular glycoprotein mRNA.
A heat-released elicitor (HRE) obtained from Sclerotinia sclerotiorum mycelium induced a transient increase in the activity of phenylalanine-ammonia lyase (PAL) in a carrot cell culture. Differential display reactions carried out on total RNA extracted from cells exposed for 7 h to the HRE revealed a complex cell response involving the induction and repression of several mRNAs. One of these elicitor-induced mRNAs encodes a 42.6-kDa protein. Northern (RNA) blot analysis and reverse-transcription polymerase chain reaction studies showed that noninduced cells have a basal expression of the 42.6-kDa protein mRNA that was stimulated five- to 10-fold by treatment with the elicitor.
Bertinetti
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Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Ugalde
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RA
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002478:Cells, Cultured; D018552:Daucus carota / Q000201:enzymology / Q000382:microbiology* / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005800:Genes, Fungal; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis*; D010940:Plant Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D019105:Random Amplified Polymorphic DNA Technique; D014158:Transcription, Genetic
C093935:EP4 glycoprotein, Daucus carota; D006023:Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; D010650:Phenylalanine Ammonia-Lyase