Flow-cytometric study of granulocyte activation during hemodialysis with different membranes.
Flow cytometry allows an easy quantitation of reactive oxygen intermediate production and of C3bi receptor expression by granulocytes, thus providing a clinical tool to evaluate the hemodialysis-related granulocyte activation. In this flowcytometric study, we analyzed the effects of cellulosic, synthetic polyacrylonitrile, and ethylene-vinyl-alcohol dialyzers on granulocyte membrane and function. Our results confirmed the data reported in the literature on granulocyte activation secondary to cellulosic membranes and the better biocompatibility of synthetic dialyzers that did not increase C3bi receptor expression and reactive oxygen intermediate generation by granulocytes. The flow-cytometric analysis of granulocyte activation might be the method of choice to identify the best patient/membrane interaction in every single patient.
Mordacchini
M
M
Blood Bank, General Hospital, Treviso, Italy.
Teodori
T
T
Collodel
L
L
Celeghin
A
A
Fregonese
A
A
Vianello
A
A
D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives*; D000368:Aged; D002482:Cellulose / Q000031:analogs & derivatives*; D003167:Complement Activation; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D011145:Polyvinyls; D017452:Receptors, IgG / Q000096:biosynthesis*; D006435:Renal Dialysis / Q000295:instrumentation*; D016897:Respiratory Burst
D000180:Acrylic Resins; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008567:Membranes, Artificial; D011145:Polyvinyls; D017452:Receptors, IgG; C020320:ethylene-vinyl alcohol copolymer; C016892:AN-69; D002482:Cellulose; C009865:cuprammonium cellulose; D000181:Acrylonitrile