Detection of potential cytokine gene(s) in an Alu-PCR library from the human chromosome 4.
Jumping and linking libraries have been constructed in order to have more information of chromosome 4. Two types of jumping libraries (Not I and Xma III jumping) and one linking library were prepared. The jumping clones represent DNA regions between two rare-cutting enzymes sites (such is Not I or Xma III) whereas linking clones represent only the area which surrounds the rare-cutting site. These clones were used as target DNA in Alu-PCR. The Alu-PCR fragments were cloned into pGEM-T vector and transformed into Escherichia coli JM 109 competent cells. The colonies were screened for inserts by restriction analysis. More than 50% of the colonies were shown to contain inserts. Inserts from each library were screened by PCR. One of the jumping libraries appeared to have inserts containing CpG islands and AT-rich sequences.
Balogh
I
I
University of Horticulture and Food Industry, Department of Microbiology, Budapest, Hungary.
Aherne
A
A
Morgan
J
J
D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D003001:Cloning, Molecular; D018899:CpG Islands; D016207:Cytokines / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D015394:Molecular Structure; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D014170:Transformation, Genetic
D016207:Cytokines; C046751:endodeoxyribonuclease XmaIII; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases