Physical analysis of the region deleted in the tw18 allele of the mouse tcl-4 complementation group.
We have generated a YAC contig of at least 3.3 Mb from the proximal region of In(17)4 of mouse chromosome 17. This region corresponds to DNA lost in the gastrulation mutant tw18, which belongs to the tcl-4 complementation group. Our most proximal and distal probes lie within the deletion-3.3 Mb apart-indicating that we have not cloned the entire region. The deleted region is contained in a genetic interval of less than 1 cM, suggesting that some suppression of recombination must occur.
Barclay
J
J
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, United Kingdom.
King
T F
TF
Crossley
P H
PH
Barnard
R C
RC
Larin
Z
Z
Lehrach
H
H
Little
P F
PF
D000483:Alleles; D000818:Animals; D002872:Chromosome Deletion; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D015342:DNA Probes; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005775:Gastrula; D005816:Genetic Complementation Test; D005819:Genetic Markers; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D015183:Restriction Mapping
D015342:DNA Probes; D005819:Genetic Markers