title: Role of 'catalytic' iron in an animal model of minimal change nephrotic syndrome.
abstract: Treatment of minimal change disease, like most glomerulonephritides, is empirical because underlying mechanisms that cause glomerular injury are not known. We examined a pathogenic role of 'catalytic' iron in a model of minimal change nephrotic syndrome induced by injection of puromycin aminonucleoside (7.5 mg/100 g body wt) to rats. Although there was no significant change in non-heme iron content in glomeruli, the bleomycin-detectable iron (capable of catalyzing free radical reactions) was markedly increased in glomeruli from nephrotic rats when compared to control. In contrast, despite a marked and significant increase in the non-heme iron content in tubules, there was no significant change in the bleomycin-detectable iron in tubules from nephrotic rats. In a separate in vivo study, the iron chelator, deferoxamine, prevented the increase in the bleomycin-detectable iron in glomeruli and provided complete protection against proteinuria. Taken together, our data suggest an important pathogenetic role for glomerular catalytic iron in the puromycin aminonucleoside-induced minimal change nephrotic syndrome.
authors.affiliation: Division of Nephrology, University of Arkansas for Medical Sciences, Little Rock, USA.
authors.forename: N
authors.initials: N
authors.lastname: Ueda
authors.forename: R
authors.initials: R
authors.lastname: Baliga
authors.forename: S V
authors.initials: SV
authors.lastname: Shah
mesh_terms: D000818:Animals; D001761:Bleomycin; D003676:Deferoxamine / Q000494:pharmacology; D004195:Disease Models, Animal; D007501:Iron / Q000032:analysis / Q000502:physiology*; D007678:Kidney Glomerulus / Q000737:chemistry / Q000378:metabolism; D007684:Kidney Tubules / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009404:Nephrotic Syndrome / Q000378:metabolism / Q000503:physiopathology*; D011507:Proteinuria; D011692:Puromycin Aminonucleoside / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017262:Siderophores / Q000494:pharmacology
chemical_list: D017262:Siderophores; D001761:Bleomycin; D011692:Puromycin Aminonucleoside; D007501:Iron; D003676:Deferoxamine