P2 purinoceptor-mediated intracellular Ca2+ transients in human sural nerve.
Segments of biopsied human sural nerve were stained with the Ca(2+)-sensitive fluorescent dyes Calcium Green-1 and Fura Red. The emission ratio was used to follow changes in the intracellular free Ca2+ concentration ([Ca2+]i). Application of ATP and analogues in concentrations between 0.3 and 300 microM via the bathing solution resulted in a transient rise in [Ca2+]i. The rank order of agonist potency, 2-methylthioATP > ATP > UTP, and the failure of adenosine, alpha,beta-MeATP and beta,gamma-MeATP to evoke rises in [Ca2+]i indicate the presence of P2Y/U subtypes of purinoceptors in this preparation. ATP-induced Ca2+ transients in biopsied human nerve preparations might serve as a diagnostic tool in neuropathies.
Wächtler
J
J
Department of Physiology, University of Munich, Germany.
Mayer
C
C
Quasthoff
S
S
Adelsberger
H
H
Grafe
P
P
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000502:physiology*; D013497:Sural Nerve / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D002118:Calcium