Ontogenic and glucocorticoid-accelerated expression of rat 14 kDa bile acid-binding protein.
BACKGROUND
A 14 kDa protein is a major rat ileal cytosolic bile acid-binding protein (14 kDa I-BABP). This report describes the normal and glucocorticoid-accelerated postnatal expression of 14 kDa I-BABP. METHODS. 14 kDa I-BABP and its mRNA were detected by antiserum and antisense cRNA probe, respectively, in ileum and ovary from 7-, 14-, 21-, 28-, and 35-day-old rats.


RESULTS
A positive histochemical reaction with 14 kDa I-BABP antiserum was found in cytosol of ileal enterocytes and ovarian luteal cells at 21 and 35 days of age, respectively. Likewise, Northern blot analysis indicated that the cRNA probe hybridized to a single transcript of 500 bp in total cellular RNA prepared from the ileum of 21-day-old and ovary of 35-day-old rats. Corticosteroid treatment resulted in a histochemical reaction in ileum of 14-day-old animals as compared to the appearance of this reaction in 21-day-old control littermates. Similarly, Northern blot analysis detected the earlier appearance of mRNA levels in corticosteroid-treated animals (11 days vs. 17 days in control animals).


CONCLUSIONS
14 kDa I-BABP and its mRNA are normally expressed in rat ileum at 17 days of postnatal life. 14 kDa I-BABP is expressed considerably later in rat ovary. Corticosteroid treatment results in precocious expression of ileal 14 kDa I-BABP.
Gong
Y Z
YZ
Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.
Kato
T
T
Schwartz
D A
DA
Norris
J S
JS
Wilson
F A
FA
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006913:Hydroxysteroid Dehydrogenases; D007082:Ileum / Q000378:metabolism*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism; D008775:Methylprednisolone / Q000494:pharmacology*; D010053:Ovary / Q000378:metabolism*; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
D002352:Carrier Proteins; D006913:Hydroxysteroid Dehydrogenases; D008562:Membrane Glycoproteins; D008775:Methylprednisolone; D018075:RNA, Complementary; D012333:RNA, Messenger; C037643:bile acid binding proteins; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)