Fluorescence behavior of tryptophan residues of bovine and human serum albumins in ionic surfactant solutions: a comparative study of the two and one tryptophan(s) of bovine and human albumins.
The fluorescence behavior of two tryptophans (Trp-134, Trp-213) in bovine serum albumin (BSA) and a single tryptophan (Trp-214) in human serum albumin (HSA) was examined. The maximum emission wavelength (lambda max) was 340.0 nm for both proteins. In a solution of sodium dodecyl sulfate (SDS), the lambda max of BSA abruptly shifted to 332 nm at 1 mM SDS and then reversed to 334 nm at 3 mM SDS. The lambda max of HSA gradually shifted to 330 nm below 3 mM SDS, although it returned to 338 nm at 10 mM SDS. In contrast to this, in a solution of dodecltrimethylammonium bromide, the lambda max positions of BSA and HSA gradually shifted to 334.0 and 331.5 nm, respectively. Differences in the fluorescence behavior of the proteins are attributed to the fact that Trp-134 exists only in BSA, with the assumption that Trp-213 of BSA behaves the same as Trp-214 of HSA. The Trp-134 behavior appears to relate to the disruption of the helical structure in the SDS solution.
Moriyama
Y
Y
Department of Applied Chemistry, Okayama University of Science, Japan.
Ohta
D
D
Hachiya
K
K
Mitsui
Y
Y
Takeda
K
K
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry* / Q000528:radiation effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000528:radiation effects; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry*; D014466:Ultraviolet Rays
D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D014364:Tryptophan; C022806:dodecyltrimethylammonium