Site-specific cleavage reaction catalyzed by leadzyme is enhanced by combined effect of lead and rare earth ions.
Lead-dependent ribozyme (leadzyme) is a ribozyme working with Pb2+. In this paper, we have investigated the combined effect of metal ions, especially rare earth ions, on the cleavage reaction by the leadzyme. As a result, it was observed that although only a rare earth ion or another divalent ion except Pb2+ did not play a role as the catalyst, the addition of a rare earth ion in the presence of Pb2+ increased significantly the yield of the cleavage reaction. The result suggests that the complex between the leadzyme and the substrate should have two classes of metal ion binding sites.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan. sugimoto@konan-u.ac.jp
Ohmichi
T
T
D001665:Binding Sites; D007477:Ions; D007854:Lead / Q000494:pharmacology*; D008674:Metals, Rare Earth / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism*; D013379:Substrate Specificity
D007477:Ions; D008674:Metals, Rare Earth; D016337:RNA, Catalytic; D007854:Lead; D012313:RNA