Immunocytochemical localization of the high-affinity glutamate transporter, EAAC1, in the retina of representative vertebrate species.
The glutamate transporter, EAAC1, was localized immunocytochemically in goldfish, salamander, turtle, chicken, and rat retinas, using affinity-purified oligopeptide antibodies. Immunoreactive (IR) EAAC1 was present in the inner plexiform layer of all species, and in cell bodies of bipolar, amacrine, and ganglion cells of most species, but absent from photoreceptors and Müller's glial cells. Western blots revealed an IR-EAAC1 band at 70 kDa. Staining was abolished by preabsorption with EAAC1 peptide.
Schultz
K
K
Lions' Sight Center and Neuroscience Research Group, University of Calgary Faculty of Medicine, Alberta, Canada. schultz@biologie.uni-oldenburg.de
Stell
W K
WK
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D002645:Chickens; D006054:Goldfish; D007150:Immunohistochemistry; D051381:Rats; D012160:Retina / Q000378:metabolism*; D014426:Turtles; D014562:Caudata
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG