title: Apical secretion of rat submandibular tissue kallikrein continues in the absence of external stimulation: evidence for a constitutive secretory pathway.
abstract: Sequential samples of saliva evoked by low frequency parasympathetic nerve stimulation were collected from cannulated submandibular ducts of anaesthetized rats following periods of varying length without stimulation. After such rest periods the first samples of saliva were found to contain much higher levels of tissue kallikrein (rK1) activity and protein than the ensuing samples from the same stimulation period and the latter contained levels similar to those found previously in parasympathetically evoked saliva rK1 activity in first samples increased with the length of the preceding interval, indicating that a continuous secretion of rK1 occurs from ductal cells into glandular lumina and accumulates there in the absence of stimulation or fluid secretion. The protein secretory pattern following rest pauses was unaffected by alpha-adrenergic receptor blockade and a high percentage of the proteinase activity was resistant to soya bean trypsin inhibitor, showing that the secretion was not caused by exocytosis of storage granules from ductal cells and therefore was likely to be a result of a constitutive secretion of newly synthesized enzyme. The pattern of continuous secretion into lumina detected for total protein in the absence of stimulation, suggests that other secretory proteins may also be secreted similarly but at different rates.
authors.affiliation: Department of Oral Pathology, King's College School of Medicine and Dentistry, London, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Garrett
authors.forename: X S
authors.initials: XS
authors.lastname: Zhang
authors.forename: G B
authors.initials: GB
authors.lastname: Proctor
authors.forename: L C
authors.initials: LC
authors.lastname: Anderson
authors.forename: D K
authors.initials: DK
authors.lastname: Shori
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007610:Kallikreins / Q000378:metabolism*; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000201:enzymology; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D012472:Salivation / Q000502:physiology*; D013363:Submandibular Gland / Q000201:enzymology* / Q000294:innervation
chemical_list: D012471:Salivary Proteins and Peptides; D007610:Kallikreins