Histocompatibility testing of dog families with highly polymorphic microsatellite markers.
The dog has served traditionally as a model for marrow and organ transplantation. A key component of any study of transplantation is histocompatibility typing of donors and recipients. Towards the development of a less expensive, more simplified typing system within canine families, a new highly polymorphic microsatellite marker for the canine Major Histocompatibility Complex class II region was isolated and characterized. In addition, we report on the application of class I and class II microsatellite-based markers for following the inheritance of the alleles within the canine analog of the human HLA loci, DLA, through multi-generation pedigree.
Wagner
J L
JL
Program in Transplantation Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Burnett
R C
RC
DeRose
S A
SA
Francisco
L V
LV
Storb
R
R
Ostrander
E A
EA
D000483:Alleles; D000818:Animals; D004285:Dogs / Q000235:genetics / Q000276:immunology*; D006650:Histocompatibility Testing / Q000662:veterinary*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic