Characterisation of human cyclin G1 and G2: DNA damage inducible genes.
Several genes have been identified as targets for transcriptional activation by the p53 tumour suppressor protein. Rodent cyclin G was previously identified as a p53 responsive gene and in order to assess the role played by cyclin G as a mediator of p53 function in humans cells we have isolated full length human cyclin G1 and identified a related gene designated cyclin G2. Both human G-cyclins are induced by the DNA damaging agent actinomycin-D and although the induction of cyclin G1 is clearly p53 dependent, activation of cyclin G2 expression was observed in the absence of p53. Based on sequence similarity, the G-cyclins and the recently identified cyclin I form a distinct sub-group within the larger cyclin family, possibly reflecting some degree of functional similarity.
Bates
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ABL-Basic Research Program, NCI-FCRDC, Frederick, Maryland 21702-1201, USA.
Rowan
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Vousden
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D000199:Actins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D056746:Cyclin G; D056766:Cyclin G1; D056767:Cyclin G2; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D003609:Dactinomycin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics
D000199:Actins; C534702:CCNG1 protein, human; C101593:CCNG2 protein, human; C493956:CDKN1A protein, human; D056746:Cyclin G; D056766:Cyclin G1; D056767:Cyclin G2; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016159:Tumor Suppressor Protein p53; D003609:Dactinomycin