Detection of California serogroup Bunyaviruses in tissue culture and mosquito pools by PCR.
A reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for rapid detection of 14 California serogroup viruses using universal primer pairs. These primers are specific for the small RNA (S RNA) and middle RNA (M RNA) segments. The method has been employed to detect Jamestown Canyon (JC) virus in naturally infected mosquitoes. With this technique, it is possible to detect virus in an amount of material equivalent to as little as 0.03 mosquito.
Campbell
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WP
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509, USA.
Huang
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D009033:Culicidae / Q000235:genetics / Q000821:virology*; D046508:Culture Techniques; D004279:DNA, Viral / Q000032:analysis; D002141:Encephalitis Virus, California / Q000235:genetics / Q000302:isolation & purification*; D007577:JC Virus / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis; D014709:Vero Cells
D004279:DNA, Viral; D012367:RNA, Viral