A Mono-Percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique.
The purpose of this study was to determine the effects of a simplified 80% Mono-Percoll sperm separation procedure on both normal and male factor semen samples compared with the standard swim-up technique. The parameters examined include sperm concentration, motility and morphology, total motile functional spermatozoa and percentage recovery. Normal patients demonstrated enhanced sperm parameters with the Mono-Percoll compared with the swim-up technique for concentration (67 x 10(6) versus 42 x 10(6)/ml, P < 0.001), motility (66 versus 59%, P < 0.001), morphology (56 versus 49%, P < 0.005) and percentage recovery (60 versus 42%, P < 0.005). Male factor patients showed enhanced sperm parameters with the Mono-Percoll procedure compared with the swim-up technique for motility (53 versus 42%, P < 0.05) and percentage recovery (54 versus 29%, P < 0.005), with no significant difference in concentration and morphology. In summary, the Mono-Percoll sperm recovery procedure is significantly better than the swim-up technique for male factor patients and patients with normal sperm parameters.
Shalika
S
S
Fertility Center of Maryland, Towson 21204, USA.
Dugan
K
K
Pelesh
D
D
Padilla
S
S
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003102:Colloids; D005069:Evaluation Studies as Topic; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone