title: Variability and repeatability of house dust mite allergen measurement: implications for study design and interpretation.
abstract: BACKGROUND
  To interpret individual measurements of house dust mite (HDM) allergen and to design and analyse HDM studies it is necessary to quantify the variability which is inherent in the measurement of this exposure.
  
  
  OBJECTIVE
  To estimate the repeatability of one method of HDM allergen measurement.
  
  
  METHODS
  We analysed data from one or more HDM allergen measurements in 215 houses included in four previous studies conducted in Sydney (a high allergen environment) and Busselton, Western Australia (a moderate allergen environment). Samples were collected from the bed by vacuuming above and below the sheets and inside the pillow case and from the bedroom and living room floors by vacuuming a 1 m2 area for 1 min. Extracts from aliquots of fine dust from each sample were assayed for HDM allergen Der p I using a monoclonal antibody enzyme linked immunosorbent assay (ELISA). The values for HDM allergen were positively skewed and the suitability of a log transformation was established by the resulting normal distribution and stable within-site variance.
  
  
  RESULTS
  The range of single determination (within which the true value lies with 95% certainty) was 3.1-fold for samples from the bed and 3.5-fold for samples from the floor. The coefficient of repeatability (the ratio beyond which a change between two estimates is established with 95% certainty) was 4.9 for the bed and 5.8 for the floor.
  
  
  CONCLUSIONS
  We estimate that, to detect a twofold difference or change in allergen levels, 35 houses per group will be required in cross-sectional studies and 30 houses per group in parallel-group, randomized controlled trials. We recommend that beds be sampled by collecting dust from the layer of bedding below the bottom sheet. A single site within the bedroom floor may be taken as representative of this site but this is not true for the living-room floor.
authors.affiliation: Institute of Respiratory Medicine, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
authors.forename: G B
authors.initials: GB
authors.lastname: Marks
authors.forename: E R
authors.initials: ER
authors.lastname: Tovey
authors.forename: J K
authors.initials: JK
authors.lastname: Peat
authors.forename: C M
authors.initials: CM
authors.lastname: Salome
authors.forename: A J
authors.initials: AJ
authors.lastname: Woolcock
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000032:analysis*; D000818:Animals; D001249:Asthma / Q000453:epidemiology* / Q000276:immunology / Q000517:prevention & control; D001512:Bedding and Linens / Q000009:adverse effects*; D002648:Child; D003430:Cross-Sectional Studies; D004391:Dust / Q000032:analysis*; D005430:Floors and Floorcoverings; D006798:Housing; D006801:Humans; D008925:Mites / Q000276:immunology*; D015588:Observer Variation; D015203:Reproducibility of Results; D018401:Sample Size
chemical_list: D000485:Allergens; D004391:Dust