Cell targeting for gene delivery: use of fusion protein containing the modified human receptor for ecotropic murine leukemia virus.
We have previously cloned a human gene (H13) homologous to the murine ecotropic retrovirus (E-MuLV) receptor, which, however, does not confer susceptibility to E-MuLV infection. The extracellular domain 3 (ECD3) of H13 contains amino acid residues critical for E-MuLV binding in that the modified H13 gene (mH13), substituted with amino acids from the actual receptor, has the ability to bind E-MuLV. Here we have expressed a fusion protein consisting of mH13/ECD3 and transforming growth factor-alpha in Escherichia coli and demonstrated its binding activity to both ecotropic AKR virus and the epidermal growth factor receptor expressed on the cell surface. Fusion proteins of mH13/ECD3 and ligands to cell surface molecules might be useful for specific cell targeting in E-MuLV-based gene delivery systems.
Ohno
K
K
Department of Pathology, New York University Medical Center, New York 10016, USA.
Brown
G D
GD
Meruelo
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting; D006801:Humans; D009052:Leukemia Virus, Murine / Q000502:physiology*; D008561:Membrane Fusion / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D016211:Transforming Growth Factor alpha / Q000235:genetics; D014407:Tumor Cells, Cultured
D004274:DNA, Recombinant; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D016211:Transforming Growth Factor alpha