A procedure for the large-scale isolation of major bovine seminal plasma proteins.
A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies.
Calvete
J J
JJ
Institut für Reproduktionsmedizin, Tierärztliche Hochschule Hannover, Bünteweg 15, Hannover, 30559, Germany.
Varela
P F
PF
Sanz
L
L
Romero
A
A
Mann
K
K
Töpfer-Petersen
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012260:Ribonucleases / Q000302:isolation & purification; D012661:Semen / Q000737:chemistry; D029589:Seminal Plasma Proteins; D019716:Tissue Inhibitor of Metalloproteinase-2
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D019716:Tissue Inhibitor of Metalloproteinase-2; D012260:Ribonucleases