New crystal forms of the motile major sperm protein (MSP) of Ascaris suum.
We have obtained two new crystal forms of the Ascaris major sperm protein (MSP) that mediates amoeboid cell motility in nematode sperm. We obtained crystals with C2 symmetry from bacterially expressed alpha-MSP with a = 216.5 A, b = 38.6 A, c = 32.5 A, gamma = 93.1 degrees and also crystals with P2(1) symmetry from native beta-MSP with a = 63.1 A, b = 91.7 A, c = 72.5 A, gamma = 91.3 degrees. A full native data set has been collected for each crystal form using synchrotron radiation. Both crystal forms diffract to 2 A and are suitable for high-resolution structural investigation.
Bullock
T L
TL
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
Parthasarathy
G
G
King
K L
KL
Kent
H M
HM
Roberts
T M
TM
Stewart
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry; D017165:Ascaris suum; D001483:Base Sequence; D003460:Crystallization; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D015801:Helminth Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000648:ultrastructure; D013081:Sperm Motility
D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D011994:Recombinant Proteins; C050602:major sperm protein, nematode