Physical and genetic chromosomal map of an M type 1 strain of Streptococcus pyogenes.
A physical map of the chromosome of an M type 1 strain of Streptococcus pyogenes was constructed following digestion with three different restriction enzymes, SmaI, SfiI, and SgrAI, and separation and analysis of fragments by pulsed-field gel electrophoresis. The genome size of this strain was estimated to be 1,920 kb. By employing Southern hybridization and PCR analysis, 36 genes were located on the map.
Suvorov
A N
AN
Institute of Experimental Medicine, Pavlova, St. Petersburg, Russia.
Ferretti
J J
JJ
D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000145:classification / Q000235:genetics*