The effect of cyclosporine A and trifluoperazine on amylase secretion from rat parotid glands in vitro.
In the present study the effects of trifluoperazine (TFP) and/or cyclosporine A (CsA) on the amylase secretion of rat parotid gland lobules were investigated. Cyclosporine A, in doses of 10(-6), 3 x 10(-6) and 10(-5) M, caused a dose-dependent reduction in amylase secretion. Trifluoperazine, a well-established calmodulin antagonist, also significantly reduced amylase secretion by the gland, confirming that the secretion is calmodulin dependent. Concurrent administration of CsA and TFP showed a marked positive interaction in reducing the secretion of amylase. Thus, the hypothesis that CsA may have Ca2+/calmodulin antagonistic properties is supported by the present study.
Samini
M
M
Department of Pharmacology, Tehran University of Medical Sciences, Iran.
Dehpour
A R
AR
Ghafourifar
P
P
Hassanzadeh
P
P
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014268:Trifluoperazine / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; D018492:Dopamine Antagonists; D007166:Immunosuppressive Agents; D014268:Trifluoperazine; D016572:Cyclosporine; D000681:Amylases; D007545:Isoproterenol