title: Isolation and characterization of a Pseudomonas aeruginosa genomic DNA sequence, encoding a putative DNA helicase belonging to superfamily I.
abstract: The isolation and characterization of a 2,122 bp DNA fragment from a Pseudomonas aeruginosa genomic library containing a 1,495 bp open reading frame (ORF) encoding a putative DNA helicase is described. The deduced amino acid (498 residues) sequence derived from this ORF, exhibited a high degree of homology to the rep and uvrD helicases of Escherichia coli (55 and 38% identities, respectively), and to those of Staphylococcus aureus and Haemophilus influenzae (43 and 39% identities, respectively), all of them belonging to superfamily I.
authors.affiliation: Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Avichezer
authors.forename: N
authors.initials: N
authors.lastname: Gilboa-Garber
authors.forename: D J
authors.initials: DJ
authors.lastname: Katcoff
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004265:DNA Helicases / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004265:DNA Helicases