rK7-kallikrein in rat kidney and urine.
Rat Kidney is reported to contain the rK7-kallikrein gene expressed at a high mRNA level. However, there is no direct evidence that the rK7 enzyme is present in kidney or urine. We have analyzed rat kidney and urine for rK7-like enzyme activity, using distinctive properties of purified rK7 enzyme (hydrolysis of specific substrate, inhibition by SBTI). Rat urine and kidney cortex contain substantial levels of rK7-like enzyme activity. Nearly 40% of the enzyme in urine is in an active form while nearly all of the rK7 in kidney cortex is in the inactive form. Treatment with low salt diet and mineralocorticoid increases rK7 in both kidney cortex and urine approximately 2-fold. The percentage of active enzyme in urine increases markedly on this regimen.
Oza
N B
NB
Division of Cardio-Renal Drug Products, HFD-110, Center for Drug Evaluation and Research, Food and Drug Administration, USPHS, Rockville, MD 20857, USA.
Weinberg
M S
MS
Oza
M N
MN
D000818:Animals; D007527:Isoenzymes / Q000652:urine*; D007610:Kallikreins / Q000737:chemistry* / Q000652:urine*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008901:Mineralocorticoids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D008901:Mineralocorticoids; D007610:Kallikreins