The thiol enzyme from rat spleen that produces bradykinin potentiating peptide from rat plasma.
We have focused our studies on a thiol-dependent enzyme of 37 kilodaltons (kDa) that produces a bradykinin (BK) potentiating peptide. The molecular mass of the peptide was estimated to be around 750 Da and its amino acid composition was Pro4 Gly2 Leu1 Ser1 with a proline and a serine at the N- and C-terminals, respectively. Biological activity was assayed by means of uterus contraction. The enzyme differs from Cathepsin L or B by virtue of its immunological reactivity and enzyme kinetics. The inhibitors used were leupeptin, antipain, E-64 and chymostatin in order of effectiveness. The low molecular SH reagents also diminished the enzyme activity. Among the metal ions tested, Cu2+ and Zn2+ inhibited the reaction.
Yamafuji
K
K
Department of Food and Nutrition, Nakamura Gakuen University, Fukuoka, Japan.
Taniguchi
Y
Y
Sakamoto
E
E
D000818:Animals; D001920:Bradykinin / Q000819:agonists / Q000096:biosynthesis; D002401:Cathepsin B / Q000032:analysis; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D004792:Enzyme Precursors / Q000032:analysis; D004798:Enzymes / Q000097:blood* / Q000502:physiology*; D009842:Oligopeptides / Q000096:biosynthesis* / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D004792:Enzyme Precursors; D004798:Enzymes; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; C006791:bradykinin potentiating factors; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534280:Ctsl protein, rat; D001920:Bradykinin