Urinary testosterone (T) to epitestosterone (E) ratios by GC/MS. I. Initial comparison of uncorrected T/E in six international laboratories.
Six laboratories in six countries collaborated to investigate the analytical method for estimating the testosterone to epitestosterone ratio (T/E) in urine by gas chromatography/mass spectrometry in the context of detecting the application of T as a doping agent in sport. The protocol specified many but not all details of reagents and instrument conditions. The design included the distribution and analysis of four urines with different T/E values, three replicates per value, and one standard. The ranges of mean T/E values for the four urines estimated by peak area (PA) were 0.32-0.42, 0.72-0.94, 0.91-1.14 and 3.19-5.48. The analyses of variance for these data and for the peak height (PH) data were significant for the laboratory factor (p < 0.0001). In addition there was a significant interaction between the urine factor and the laboratory factor which indicates the complexity of the analysis. T/E calculated using PA was not significantly different from that using PH. For within-laboratory precision all values for PH and PA were < 8.3%, and for between-laboratory precision all values were < 11.7% except for one (20.1%). The data represent a baseline for future experiments designed to elucidate the sources of within-and between-laboratory variance, and to harmonize estimates of T/E.
Catlin
D H
DH
Department of Molecular and Medical Pharmacology, University of California at Los Angeles, USA.
Cowan
D A
DA
de la Torre
R
R
Donike
M
M
Fraisse
D
D
Oftebro
H
H
Hatton
C K
CK
Starcevic
B
B
Becchi
M
M
de la Torre
X
X
Norli
H
H
Geyer
H
H
Walker
C J
CJ
D000704:Analysis of Variance; D004300:Doping in Sports; D004845:Epitestosterone / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D007753:Laboratories; D012015:Reference Standards; D015203:Reproducibility of Results; D013739:Testosterone / Q000652:urine*
D007202:Indicators and Reagents; D013739:Testosterone; D004845:Epitestosterone