title: [The use of the polymerase chain reaction for the identification of Clostridium botulinum type A strains].
abstract: Polymerase chain reaction (PCR) for the identification of C.botulinum of type A was developed. As primers, oligonucleotides corresponding to sequences 913 -- 932 and 1852 -- 1871 of the gene of type A botulinic neurotoxin were used. The study revealed that under optimum conditions the positive result of the reaction was registered only when the DNA of C.botulinum strains of type A (11 strains) was used, but not that of C.botulinum strains of other types (11 strains of type B, 5 strains of type C, 2 strains of type D, 6 strains of type E and 1 strain of type G). High sensitivity, specificity and rapidity of PCR open good prospects for its practical use.
authors.affiliation: Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Shliapnikova
authors.forename: A N
authors.initials: AN
authors.lastname: Efimenko
authors.forename: T I
authors.initials: TI
authors.lastname: Bulatova
authors.forename: M E
authors.initials: ME
authors.lastname: Liaĭman
authors.forename: Y V
authors.initials: YV
authors.lastname: Vertiev
mesh_terms: D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics; D003014:Clostridium botulinum / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D001905:Botulinum Toxins