title: Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.
abstract: A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
authors.affiliation: Medical Research Council Laboratories, Fajara, The Gambia, West Africa.
authors.forename: M
authors.initials: M
authors.lastname: Hassan-King
authors.forename: I
authors.initials: I
authors.lastname: Baldeh
authors.forename: R
authors.initials: R
authors.lastname: Adegbola
authors.forename: C
authors.initials: C
authors.lastname: Omosigho
authors.forename: S O
authors.initials: SO
authors.lastname: Usen
authors.forename: A
authors.initials: A
authors.lastname: Oparaugo
authors.forename: B M
authors.initials: BM
authors.lastname: Greenwood
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D003470:Culture Media; D005714:Gambia / Q000453:epidemiology; D006192:Haemophilus Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D011008:Pneumococcal Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D018410:Pneumonia, Bacterial / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media