Changes in chromosomal ultrastructure during the cell cycle.
The surface structure of mitotic barley and rye chromosomes was studied by high-resolution scanning electron microscopy. Chromosomes with various degrees of chromatin condensation were prepared from untreated meristematic tissue of root tips. At lower magnifications the highly condensed chromosomes in metaphase and anaphase showed a compact structure with a smooth surface. The condensation starts from the centromeric region and the chromatids are often discernible in the still uncondensed telomeric region. Decondensation begins at the telomeric region during telophase. Parallel arrangement of fibres is a characteristic feature predominately seen in prophase and telophase chromosomes. Chromatin structures that resemble tiles on a roof or braided strands were often observed. Prophase and telophase chromosomes are particularly suitable for further studies of chromatin arrangement and organization in plant chromosomes.
Martin
R
R
Botanisches Institut der Ludwig-Maximilians-Universität, Munich, Germany. barley@botanik.biologie.uni-muenchen.de
Busch
W
W
Herrmann
R G
RG
Wanner
G
G
D002453:Cell Cycle; D002842:Chromatids / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D001467:Hordeum / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D012434:Secale / Q000166:cytology*