[Direct determination of ABO and cisAB blood group genotypes using polymerase chain reaction amplification of specific alleles (PASA)--method].
The genotypes of genomic DNAs of 20 normal subjects with ABO blood group and 12 subjects with cisAB blood group were directly determined using polymerase chain reaction (PCR) amplification of specific alleles (PASA)-method. This method is based on the fact that PCR amplification occurs only when the 3' endbase of the primer is matched to the nucleotide of No. 261, 526, 796 or 803 of ABO allelic cDNA. And three of five regions of allelic DNAs are co-amplified in a single PCR (multiplex-PCR) in this study. ABO and cisAB blood group genotypes are directly determined, based on the molecular size of allele specific amplification products that contain 261, 526, 796 and 803 nucleotide (the sites of amino acid substitutions). The method requires only about 4 hours from starting up of PCR to the results, so it is rapid, simple and useful for detecting the genotype of ABO and cisAB blood groups.
Hosoi
E
E
Department of Medical Technology, School of Medical Sciences, University of Tokushima.
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D004247:DNA / Q000032:analysis; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D000017:ABO Blood-Group System; D004247:DNA