title: Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I.
abstract: DNA repair in the eukaryotic cell disrupts local chromatin organization. To investigate whether the resetting of nucleosomal arrays can be linked to the repair process, we developed model systems, with both Xenopus egg extract and human cell extracts, to follow repair and chromatin assembly in parallel on circular DNA templates. Both systems were able to carry out nucleotide excision repair of DNA lesions. We observed that UV-dependent DNA synthesis occurs simultaneously with chromatin assembly, strongly indicating a mechanistic coupling between the two processes. A complementation assay established that chromatin assembly factor I (CAF1) is necessary for this repair associated chromatin formation.
authors.affiliation: Institut Curie/Research section UMR144 du CNRS, Paris, France.
authors.forename: Pierre-Henri L
authors.initials: PHL
authors.lastname: Gaillard
authors.forename: E M
authors.initials: EM
authors.lastname: Martini
authors.forename: P D
authors.initials: PD
authors.lastname: Kaufman
authors.forename: B
authors.initials: B
authors.lastname: Stillman
authors.forename: E
authors.initials: E
authors.lastname: Moustacchi
authors.forename: G
authors.initials: G
authors.lastname: Almouzni
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism / Q000528:radiation effects; D011994:Recombinant Proteins / Q000378:metabolism; D014466:Ultraviolet Rays; D014981:Xenopus
chemical_list: D002843:Chromatin; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins