title: Rapid, solid phase extraction technique for the high-throughput assay of darifenacin in human plasma.
abstract: A novel method has been developed for the rapid solid phase extraction of drugs and metabolites from biological fluids, prior to further analysis. The newly designed, 96-tube micropreparation block facilitates high throughput by enabling the extraction of 96 samples simultaneously. The system is described, linked to HPLC/APCI-MS/MS, for the determination of darifenacin in human plasma. The resulting procedure, using deuterated darifenacin as internal standard, is validated over the concentration range 25-2000 pg/mliter; accuracy (0.6-4.6%) and precision (3.6-18.8%) are considered acceptable and overall recovery was determined to be approximately 50%.
authors.affiliation: Department of Drug Metabolism, Pfizer Central Research, Sandwich, Kent, U.K.
authors.forename: B
authors.initials: B
authors.lastname: Kaye
authors.forename: W J
authors.initials: WJ
authors.lastname: Herron
authors.forename: P V
authors.initials: PV
authors.lastname: Macrae
authors.forename: S
authors.initials: S
authors.lastname: Robinson
authors.forename: D A
authors.initials: DA
authors.lastname: Stopher
authors.forename: R F
authors.initials: RF
authors.lastname: Venn
authors.forename: W
authors.initials: W
authors.lastname: Wild
mesh_terms: D001572:Benzofurans / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011759:Pyrrolidines / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D001572:Benzofurans; D011759:Pyrrolidines; C101207:darifenacin