Escherichia coli strains in which chromosome replication is controlled by a P1 or F replicon integrated into oriC.
We report the construction of intP1 and intFs strains, in which the basic replicon from either plasmid P1 or plasmid F (oriS) has been integrated in both orientations into the origin of replication, oriC, of the Escherichia coli chromosome. In these strains, oriC is no longer functional and chromosome-replication is instead controlled by the integrated plasmid replicon. The strains were viable, showing that the deviation from normal chromosome-replication control was not large enough to prohibit cell survival. The strains showed a broader cell-size distribution than a wild-type strain and were more filamentous in rich than in minimal media, although cells of wild-type size were also present. Cells which contained aberrantly shaped or aberrantly distributed nucleoids were also observed. Marker-frequency analysis indicated that chromosome replication was predominantly bidirectional in both intFs strains. In the intP1 strains, the degree of bidirectionality depended upon the orientation of the integrated replicon.
Eliasson
A
A
Department of Microbiology, Uppsala University, Sweden.
Nordström
K
K
Bernander
R
R
D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004261:DNA Replication; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D005819:Genetic Markers; D016254:Mutagenesis, Insertional; D018741:Replication Origin; D012093:Replicon
D004269:DNA, Bacterial; D005819:Genetic Markers