Establishment of Bcgr congenic mice and their susceptibility/resistance to mycobacterial infection.
Bcg congenic mice were developed by using C57BL/6 and DBA/2 strains of mice as progenitors. They were obtained by introgressively backcrossing the Bcgr marker of DBA/2 onto C57BL/6. After twenty successive backcrossings, the heterozygous resistant mice were mated with each other to obtain homozygous mice as the Bcgr congenic mice. The results of immunogenic and genetic markers coupled with those of an mixed lymphocyte reaction, all confirmed that the newly developed mice were highly congenic. These congenic mice were found to be resistant to in vivo infections by Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium bovis BCG.
Xu
D L
DL
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
Goto
Y
Y
Amoako
K K
KK
Nagatomo
T
T
Fujita
T
T
Shinjo
T
T
D000818:Animals; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009164:Mycobacterium Infections / Q000235:genetics / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000235:genetics*