Capillary electrophoretic enantioseparation of selegiline, methamphetamine and ephedrine using a neutral beta-cyclodextrin epichlorhydrin polymer.
This paper describes the development of a capillary zone electrophoretic method for chiral separation of three basic compounds of the selegiline synthetic pathway: ephedrine, methamphetamine and selegiline. The method developed allows one to separate the studied compounds in one run using a neutral beta-cyclodextrin epichlorhydrin polymer. The effect of various experimental parameters, such as chiral selector concentration, concentration and composition of background electrolyte, pH, temperature, and the addition of some organic solvents, on the resolution and migration time is discussed. For selegiline and methamphetamine, it is possible, under optimal conditions, to quantify less than 0.5% of the minor isomer in an excess of the major one.
Sevcík
J
J
Department of Analytical and Organic Chemistry, Palacký University, Olomouc, Czech Republic. sevcik@risc.upol.cz
Stránský
Z
Z
Ingelse
B A
BA
Lemr
K
K
D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D004809:Ephedrine / Q000302:isolation & purification*; D004811:Epichlorohydrin; D006863:Hydrogen-Ion Concentration; D013287:Illicit Drugs / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011108:Polymers; D012642:Selegiline / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D013287:Illicit Drugs; D007202:Indicators and Reagents; D011108:Polymers; D047392:beta-Cyclodextrins; C102558:cyclodextrin-epichlorohydrin polymer; D004811:Epichlorohydrin; D012642:Selegiline; D008694:Methamphetamine; D004809:Ephedrine; C031215:betadex