Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis.
The relative immunogenicity of tetanus toxin fragment C (TTFC) has been determined in three different strains of inbred mice when expressed in Lactococcus lactis as a membrane-anchored protein (strain UCP1054), as an intracellular protein (strain UCP1050), or as a secreted protein which is partly retained within the cell wall (strain UCP1052). Protection against toxin challenge (20 x LD50) could be obtained without the induction of anti-lactococcal antibodies. When compared in terms of the dose of expressed tetanus toxin fragment C required to elicit protection against lethal challenge the membrane-anchored form was significantly (10-20 fold) more immunogenic than the alternative forms of the protein.
Norton
P M
PM
Department of Pathology, University of Cambridge, UK.
Brown
H W
HW
Wells
J M
JM
Macpherson
A M
AM
Wilson
P W
PW
Le Page
R W
RW
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002451:Cell Compartmentation; D005260:Female; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000627:therapeutic use; D013742:Tetanus / Q000517:prevention & control; D013744:Tetanus Toxin / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use / Q000633:toxicity; D013745:Tetanus Toxoid / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
D000907:Antibodies, Bacterial; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C