A protein synthesis-dependent late phase of cerebellar long-term depression.
Synthesis of new protein has been shown to be required for establishment of a late phase in hippocampal long-term potentiation. Whether a similar requirement is needed to produce the late phase of long-term depression (LTD) remains to be determined. Application of transcription inhibitors or of the translation inhibitor anisomycin, immediately, but not 30 min after glutamate/depolarization conjunction, attenuated a late phase of cerebellar LTD in culture. LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, which lacks nuclear material, returned to baseline values with a time course paralleling that observed with protein synthesis inhibitors in intact cultured Purkinje neurons. These findings suggest that there is a distinct late phase of cerebellar LTD that is dependent upon postsynaptic protein synthesis.
Linden
D J
DJ
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D018698:Glutamic Acid / Q000502:physiology; D015640:Ion Channel Gating / Q000502:physiology; D051379:Mice; D009433:Neural Inhibition / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D018408:Patch-Clamp Techniques; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011689:Purkinje Cells / Q000502:physiology; D014158:Transcription, Genetic / Q000187:drug effects
D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D018698:Glutamic Acid; D000841:Anisomycin