title: Characterization of Borrelia burgdorferi sensu lato strains isolated from Ixodes ricinus in Mecklenburg-Vorpommern, Germany.
abstract: Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence of Borrelia burgdorferi-infected ticks. A total of 17 B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal anti-bodies against different epitopes of the outer surface protein A (OspA). Except for one, all strains could be classified using this system. The majority of strains belonged to the B. garinii-associated OspA serotypes 3, 5 and 6. Three isolates were classified as OspA serotype 2 (B. afzelii). B. burgdorferi sensu stricto strains (OspA serotype 1) as well as B. garinii-associated OspA serotype 4 were not present.
authors.affiliation: Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lottmann
authors.forename: B
authors.initials: B
authors.lastname: Wilske
authors.forename: H
authors.initials: H
authors.lastname: Herrmann
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000939:Epitopes; D005858:Germany / Q000453:epidemiology; D018884:Ixodes / Q000382:microbiology*; D008074:Lipoproteins; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; D008074:Lipoproteins; C072045:OspA protein