DNA typing by triplex affinity capture (TAC) PCR.
Using a triplex affinity capture approach, we extracted PCR-ready DNA from fresh blood, buccal swabs, and bloodstains made on hard objects. We immobilized a biotinylated purine-rich oligonucleotide onto streptavidin-coated magnetic beads, and captured double-stranded DNA via triple-helix formation. We were able to amplify the MCT118, ACTBP2, FGA, D8S320, D11S488, and THO1 loci from the captured DNA. DNA typing by triplex affinity capture PCR is simple, rapid, and well-suited for paternity testing and population studies of microsatellites.
Kishida
T
T
Department of Forensic Medicine, Oita Medical University, Japan.
Fukuda
M
M
Okazaki
K
K
Wang
W
W
Tamaki
Y
Y
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA