Excursions in biomedical mass spectrometry.
1. Mass spectrometry (MS) has played a vital role in the research of the department of clinical pharmacology for over 25 years. 2. MS has been used for trace analysis of endogenous compounds and xenobiotics in plasma and urine, and also for a wide range of structural studies. 3. Examples of current applications are reported, including data from gas chromatography-mass spectrometry (GC-MS) assays for mevalonic acid, the identification of an antibiotic produced by Pseudomonas aeruginosa which is active against Helicobacter pylori, high pressure liquid chromatography (h.p.l.c)-electropray MS studies on steroid sulphates, the aspergillus ciliotoxin, gliotoxin, and ADP ribosyltransferase activity in human polymorphonuclear neutrophil leucocytes (PMNs). The value of electrospray MS in the molecular weight determination of proteins is exemplified by the analysis of human serum amyloid component P.
Taylor
G W
GW
Department of Clinical Pharmacology. Royal Postgraduate Medical School, London, UK.
Donnelly
L E
LE
Murray
S
S
Rendell
N B
NB
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002784:Cholesterol / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D016480:Helicobacter pylori / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002784:Cholesterol; D008798:Mevalonic Acid