PCR-SSCP analysis of the p53 gene in tumours of the adrenal gland.
1. Mutations of the p53 tumour suppressor gene are relatively common in the aetiology of a wide spectrum of tumour types, both sporadic and familial. 2. The majority of mutations of the p53 gene are reported to be in the highly conserved region of exons 5-8. 3. Alterations in exons 4, 5 and 7 of the p53 gene in patients with functional adrenal tumours, including aldosterone-producing adenomas, have recently been described. 4. In the present study PCR-SSCP was used to examine the exons 4-9 of the p53 gene in paired peripheral blood leucocyte and tumour DNA in a variety of adrenal tumours, including aldosterone-producing carcinoma and adenoma (both familial and sporadic), phaeochromocytoma and incidentaloma. 5. No evidence was found for mutations in exons 4-9 of the p53 gene in these varieties of adrenal tumours.
Ballantine
D M
DM
Hypertension Unit, Greenslopes Hospital, Brisbane, Queensland, Australia.
Klemm
S A
SA
Tunny
T J
TJ
Stowasser
M
M
Gordon
R D
RD
D000310:Adrenal Gland Neoplasms / Q000235:genetics*; D000450:Aldosterone / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D005091:Exons / Q000502:physiology; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm; D000450:Aldosterone