title: Insect neuropeptide F (NPF)-related peptides: isolation from Colorado potato beetle (Leptinotarsa decemlineata) brain.
abstract: Two novel neuropeptides with neuropeptide F (NPF)-like immunoreactivity have been isolated from brain extracts of the Colorado potato beetle. Purification was achieved primarily by use of reverse phase chromatography including initial C-18 Sep-Pak cartridges and 4 subsequent analytical HPLC columns. Combined data from automated Edman degradation, immunochemical analysis, u.v. absorbance and mass spectrometry led to the elucidation of their full primary structures. The deduced sequences are: Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-NH2 (ARGPQLRLRFamide) and Ala-Pro-Ser-Leu-Arg-Leu-Arg-Phe-NH2 (APSLRLRFamide). On the basis of their primary structure both peptides can be appended to the invertebrate group of neuropeptide Y (NPY)-like peptides, generally referred to as NPFs. We suggest these peptides to be designated Led-NPF-1 and Led-NPF-2.
authors.affiliation: Zoological Institute of the University, Leuven, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Spittaels
authors.forename: P
authors.initials: P
authors.lastname: Verhaert
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: R N
authors.initials: RN
authors.lastname: Johnston
authors.forename: B
authors.initials: B
authors.lastname: Devreese
authors.forename: J
authors.initials: J
authors.lastname: Van Beeumen
authors.forename: A
authors.initials: A
authors.lastname: De Loof
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D001517:Coleoptera / Q000737:chemistry*; D017952:Ganglia, Invertebrate / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*; D011863:Radioimmunoassay; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009479:Neuropeptides; C076101:neuropeptide F