Conditions affecting the isolation of human umbilical cord blood CD34+ cells.
The magnetic cell sorting (MACS) system was used to isolate CD34+ human umbilical cord blood (HUCB) stem cells (SC). Variables that might affect the detection of CD34+ cells, purity, enrichment, and yield of CD34+ cells, and the co-expression by CD34+ cells of CD38 and HLA-DR and their clonogenic capabilities were investigated. These variables were (a) separation technique [Ficoll, Percoll mononuclear cell (MNC) preparation] and (b) storage of HUCB [fresh, stored overnight (O/N) at 4 degrees C or frozen]. There was no difference between MNC prepared for MACS CD34+ SC isolation by Ficoll or Percoll separation. The use of fresh, O/N, or frozen HUCB did not affect the isolation of CD34+ SC.
Case
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Bone Marrow Transplant Laboratory, Prince of Wales Children's Hospital, Randwick, New South Wales, Australia.
Rice
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A
Vowels
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D018952:Antigens, CD34 / Q000032:analysis*; D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006410:Hematopoiesis; D006801:Humans; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology
D018952:Antigens, CD34