Helicobacter pylori isolated from stomach corpus and antrum: comparison of DNA patterns.
The genomic DNA of Helicobacter pylori was studied in strains isolated from two different sites of the stomach: the corpus and the antrum. 70 strains of H. pylori were found in 36 patients; 34 out of the 36 patients harboured the strain in both districts analysed. Restriction endonuclease analysis with Hae III and Hind III was used to compare the DNA patterns of strains isolated from the anatomical sites studied. Two pairs of DNA samples were not digested by these enzymes. 27 of the 32 pairs of the digested DNA appeared similar to each other. The analysis of chromosomal DNA in the remaining five pairs showed different electrophoretic patterns. These results indicate that the gastric mucosa can be colonized, at the same time, by strains of H. pylori with different genomic patterns, and this aspect can be important for epidemiological studies.
Cellini
L
L
Istituto di Medicina Sperimentale, Facoltà di Medicina, Università G. D'Annunzio, Chieti, Italy.
Allocati
N
N
Campli
E D
ED
Masulli
M
M
DiBartolomeo
S
S
Dainelli
B
B
D000328:Adult; D000367:Age Factors; D004269:DNA, Bacterial / Q000032:analysis*; D004415:Dyspepsia / Q000382:microbiology; D005260:Female; D005748:Gastric Fundus / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011706:Pyloric Antrum / Q000382:microbiology; D012737:Sex Factors; D013270:Stomach / Q000382:microbiology*
D004269:DNA, Bacterial