title: Amiodarone affects membrane water permeability properties of human erythrocytes and rat mitochondria.
abstract: Dose-dependent water exchange times and intracellular water contents were measured by NMR (nuclear magnetic resonance) in erythrocytes and mitochondria interacted with the anti-anginal and anti-arrhytmic agent, amiodarone. Addition of the drug up to 26 microM yielded 80% enhancement of the water exchange rate in erythrocytes at 37 degrees C and 41% enhancement at 22 degrees C with 40% and 9%, respectively, increases in the intracellular water content. Similar enhancements were obtained in mitochondria at 22 degrees C. The data suggests a somewhat higher affinity of amiodarone to mitochondrial than to erythrocyte membranes.
authors.affiliation: Institute of Neurological Sciences, University of Siena, Nuovo Policlinico, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Federico
authors.forename: C
authors.initials: C
authors.lastname: Battisti
authors.forename: L
authors.initials: L
authors.lastname: Manneschi
authors.forename: E
authors.initials: E
authors.lastname: Gaggelli
authors.forename: M
authors.initials: M
authors.lastname: Tassini
authors.forename: G
authors.initials: G
authors.lastname: Valensin
authors.forename: A
authors.initials: A
authors.lastname: Vivi
mesh_terms: D000638:Amiodarone / Q000494:pharmacology*; D000818:Animals; D001834:Body Water / Q000737:chemistry / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000638:Amiodarone