Rapid antigen detection assay for identification of Burkholderia (Pseudomonas) pseudomallei infection.
A simple antigen detection test was developed for rapid diagnosis of melioidosis caused by Burkholderia (Pseudomonas) pseudomallei infection. The method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for B. pseudomallei. The results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.
Anuntagool
A
A
Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand.
Intachote
P
P
Naigowit
P
P
Sirisinha
S
S
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D016957:Burkholderia pseudomallei / Q000276:immunology* / Q000302:isolation & purification*; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial