Thermodynamic and fluorescence studies of the underlying factors in benzyl alcohol-induced lipid interdigitated phase.
To further investigate factors contributing to the action of alcohol in the solute-induced lipid interdigitation phase, thermodynamic and fluorescence polarization measurements were carried out to study the interaction of benzyl alcohol with dipalmitoyl phosphatidylcholine bilayer vesicles. The obtained results were compared with those previously reported for ethanol and cyclohexanol (L. G. Roth and C-H. Chen, Arch. Biochem. Biophys. 296, 207, 1992). Similar to ethanol, benzyl alcohol was found to exhibit a biphasic effect on the enthalpy (delta Hm) and the temperature (tm) of the lipid-phase transition and the steady-state fluorescence polarization (P) monitored by 1,6-diphenyl-1,3,5-hexatriene. At a total concentration of benzyl alcohol < 30 mg/ml (the alcohol concentration in lipid phase < 21 mg/ml), benzyl alcohol was found to exhibit large increases in delta Hm and P, which were correlated with the formation of a lipid interdigitated phase, as evidenced by reported X-ray diffraction data. Combining the results with benzyl alcohol and ethanol suggested that simultaneously large changes in delta Hm and P can be used as an indication of the occurrence of a solute-induced lipid interdigitated phase. The overall interacting force in the formation of this lipid phase, as derived from the interactions of the hydroxyl portion of an alcohol with the lipid phosphate head group and the hydrophobic portion of an alcohol with the lipid hydrocarbon chains, may or may not be dominated by hydrophobic interaction. Although lipid/water partition coefficients and the contribution of hydrophobic interaction to the overall interacting force were comparable between benzyl alcohol and cyclohexanol, benzyl alcohol induced lipid interdigitated phase, but not for cyclohexanol. This was due to the ability of benzyl alcohol to be more effective than cyclohexanol in simultaneously interacting with the phosphate head group and the hydrocarbon chains of lipid.
Chen
C H
CH
Wadsworth Center, New York State Department of Health, Albany, USA.
Hoye
K
K
Roth
L G
LG
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols; D002152:Calorimetry, Differential Scanning; D003511:Cyclohexanols; D004161:Diphenylhexatriene; D000431:Ethanol; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014961:X-Ray Diffraction
D001592:Benzyl Alcohols; D003511:Cyclohexanols; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D004161:Diphenylhexatriene; D015060:1,2-Dipalmitoylphosphatidylcholine; D000431:Ethanol; D019905:Benzyl Alcohol