Uso1 protein is a dimer with two globular heads and a long coiled-coil tail.
USO1 is one of the essential genes in Saccharomyces cerevisiae whose gene products participate in protein transport from the endoplasmic reticulum to the Golgi apparatus. This product was purified to homogeneity. Electron microscopic study revealed that it has a single or double globular domain with a long tail and that the molecule is a dimer. A peak position of the distribution of rod length was 154.5 nm, in agreement with the secondary structure prediction that it has a long alpha-helix at the carboxyl terminus. Probability of coiled-coil formation was also predicted from the primary structure of the product, which asserts that it has a long alpha-helical coiled-coil at the carboxyl-terminal region with some interruptions. Certainly, the electron microscopic image of this molecule had some hinges within the rod region. The distance was measured between the globular domain and the hinges. Two peaks of the distribution of the hinge position exist at 23.1 and 85.5 nm from the globular domain. This is consistent with the predicted positions of interruption. These results give new experimental evidence that Uso1 protein is a dimer and has an alpha-helical coiled-coil tail with two globular heads.
Yamakawa
H
H
Department of Physics, School of Science, University of Tokyo, Japan. yamakawa@kazusa.or.jp
Seog
D H
DH
Yoda
K
K
Yamasaki
M
M
Wakabayashi
T
T
D002352:Carrier Proteins; D002852:Chromatography, Ion Exchange; D005583:Fourier Analysis; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D005800:Genes, Fungal; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D005656:Fungal Proteins; D046911:Macromolecular Substances; D029701:Saccharomyces cerevisiae Proteins; C086888:USO1 protein, S cerevisiae; D033921:Vesicular Transport Proteins