Frequency and specificity of platelet-specific alloantibodies in HLA-immunized haematologic-oncologic patients.
The frequency and specificity of platelet-alloantibodies to human platelet antigens (HPA) -1, -3 and -5 was investigated in 59 multitransfused, HLA-immunized patients. Using the MAIPA test (monoclonal antibody specific immobilization of platelet antigens) platelet alloantibodies could be demonstrated in 10 (17%) patients. In one patient the antibody was present prior to any transfusions and probably induced by multiple previous pregnancies. This antibody was directed to HPA-5b. The remaining nine antibodies were found in patients (n = 36) with HLA-antibodies reacting with over 95% of unselected lymphocytes. In these patients the target antigens were HPA-1b in six, HPA-3a in one and both antigens in two patients. Our findings demonstrate platelet alloimmunization induced by transfusions to be restricted to patients with high HLA-immunization. 25% of these patients (9/36) show platelet-specific antibodies, primarily HPA-1b.
Schnaidt
M
M
Department of Transfusion Medicine, University Hospital Tübingen, Germany.
Northoff
H
H
Wernet
D
D
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D016824:Antigens, Human Platelet / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D019337:Hematologic Neoplasms / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007118:Immunoassay / Q000379:methods; D007518:Isoantibodies / Q000097:blood*; D065227:Transfusion Reaction
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D006680:HLA Antigens; D007518:Isoantibodies