title: Effect of developed tension on the time courses of Ca2+ transients and tension in twitch contraction in ferret myocardium.
abstract: OBJECTIVE
  The aim of the study is to test the hypothesis that tension-dependent change in the affinity of cardiac troponin-C influences the time courses of Ca2+ transients and tension in twitch contraction.
  
  
  METHODS
  The Ca(2+)-sensitive photoprotein, aequorin, was microinjected into superficial cells of ferret papillary muscles and the Ca2+ transients and tension were simultaneously measured. The peak of developed tension was altered by changing the extracellular Ca2+ concentration, initial muscle length, and the application of 2,3-butanedione monoxime.
  
  
  RESULTS
  In each maneuver, the decay time of Ca2+ transients was prolonged and the tension relaxation time was shortened when the peak of developed tension was decreased. In contrast, when the peak of developed tension was increased, the decay time of Ca2+ transients was shortened and the tension relaxation time was prolonged. The decay time of Ca2+ transients measured with different maneuvers was negatively correlated with the peak tension and the tension relaxation time was positively correlated with the tension peak.
  
  
  CONCLUSIONS
  The changes in the decay time of Ca2+ transients and the tension relaxation time indicate that developed tension modulates the affinity of troponin-C for Ca2+ in normal twitch contraction.
authors.affiliation: Department of Physiology, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Komukai
authors.forename: S
authors.initials: S
authors.lastname: Kurihara
mesh_terms: D000331:Aequorin; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002801:Cholinesterase Reactivators / Q000494:pharmacology; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology; D005289:Ferrets; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism*; D013997:Time Factors; D014336:Troponin / Q000378:metabolism
chemical_list: D002801:Cholinesterase Reactivators; D014336:Troponin; C004717:diacetylmonoxime; D000331:Aequorin; D003931:Diacetyl; D002118:Calcium