Detection of fast capillary electrophoresis peptide and protein separations using electrospray ionization with a time-of-flight mass spectrometer.
Fast capillary electrophoresis (CE) separations on bare and coated fused silica have been detected on-line using a time-of-flight mass spectrometer which is capable of acquiring spectra at a rate suitable for the narrow peaks generated by CE. Protein and peptide separations producing peaks 1-2 s in width have been detected with an integration time of 0.125 s/spectrum, each spectrum being the sum of 1024 complete m/z range scans. The effects of the liquid sheath flow rate, electric field strength, and integration time on sensitivity and peak shape have been examined. A sensitivity limit of 8 fmol has been established for leucine enkephalin.
Banks
J F
JF
Analytica of Branford, Connecticut 06405, USA.
Dresch
T
T
D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
D000588:Amines; D010455:Peptides; D011506:Proteins; D012822:Silicon Dioxide