Immunoglobulin A-specific serodiagnosis of acute human cytomegalovirus infection by using recombinant viral antigens.
The immunoglobulin A-specific reactivities of recombinant viral proteins from nine different reading frames of human cytomegalovirus were evaluated in enzyme-linked immunosorbent assay experiments. Antigen fragments of reading frames pUL32, pUL44, and pUL57 were identified as preferable antigens for immunoglobulin A serodiagnosis. Application of autologous fusion proteins which combine these polypeptides may be useful especially for the early detection of acute secondary human cytomegalovirus infection.
Vornhagen
R
R
Research Department, Biotest AG, Dreieich, Germany.
Hinderer
W
W
Sonneborn
H H
HH
Bein
G
G
Matter
L
L
The
T H
TH
Jahn
G
G
Plachter
B
B
D000208:Acute Disease; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D016364:Reading Frames; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007070:Immunoglobulin A; D011993:Recombinant Fusion Proteins