Effect of beta-cyclodextrin derivatives on the retention of steroidal drugs.
The retention of eighteen steroid drugs was determined on a beta-cyclodextrin polymer (beta CDP)-coated silica column using methanol-water mixtures as eluents. The relative strength of inclusion formation between the drugs and hydroxypropyl-beta CD (HP beta CD) and dimethyl-beta CD was determined by charge transfer chromatography carried out on reversed-phase thin-layer chromatography plates. The retention characteristics of drugs were correlated with their physicochemical parameters and with their inclusion complex-forming capacity. Calculations indicated that the inclusion complex-forming capacity of the drugs has little impact on the retention that is due to the HP beta CD and water-insoluble beta CD polymers exposed to different retention characteristics. The hydrophilic molecular parameters of drugs significantly influenced their retention. This result suggests that the selectivity of the beta CDP-coated column may be different from that of the traditional reversed-phase columns.
Cserháti
T
T
Central Research Institute of Chemistry, Hungarian Academy of Sciences, Budapest, Hungary.
Forgács
E
E
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D008863:Microspheres; D008956:Models, Chemical; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry*; D013256:Steroids / Q000032:analysis* / Q000737:chemistry*
D003505:Cyclodextrins; D011108:Polymers; D013256:Steroids; D000432:Methanol