title: Mechanistic studies of partial-filling micellar electrokinetic chromatography.
abstract: The need for coupling micellar electrokinetic chromatography (MEKC) with electrospray mass spectrometry initiates the development of partial-filling MEKC. In comparison with conventional MEKC, only a small portion of the capillary is filled with a micellar solution for performing the separation in partial-filling MEKC. Analytes first migrate into the micellar plug, where the separation occurs, and then into the leading electrophoresis buffer, which is free of surfactants. A theoretical model is proposed for predicting the separation behavior of triazine herbicides in partial-filling MEKC. The comparisons between conventional and partial-filling MEKC in terms of separation efficiency and resolution of triazine herbicides are presented and discussed. The optimization techniques, possible applications, and advantages of partial-filling MEKC are similarly addressed.
authors.affiliation: Department of Chemistry, Iowa State University, Ames 50011, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Nelson
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
mesh_terms: D002845:Chromatography / Q000379:methods*; D004563:Electrochemistry; D006540:Herbicides / Q000032:analysis; D007700:Kinetics; D008823:Micelles
chemical_list: D006540:Herbicides; D008823:Micelles