title: Beta-carotene and lutein protect HepG2 human liver cells against oxidant-induced damage.
abstract: Numerous epidemiological studies support a strong inverse relationship between consumption of carotenoid-rich fruits and vegetables and the incidence of some degenerative diseases. One proposed mechanism of protection by carotenoids centers on their putative antioxidant activity, although direct evidence in support of this contention is limited at the cellular level. The antioxidant potential of beta-carotene (BC) and lutein (LUT), carotenoids with or without provitamin A activity, respectively, was evaluated using the human liver cell line HepG2. Pilot studies showed that a 90-min exposure of confluent cultures to 500 mumol/L tert-butylhydroperoxide (TBHP) at 37 degrees C significantly (P < 0.05) increased lipid peroxidation and cellular leakage of lactate dehydrogenase (LDH), and decreased the uptake of 3H-alpha-aminoisobutyric acid and 3H-2-deoxyglucose. Protein synthesis, mitochondrial activity and glucose oxidation were not affected by TBHP treatment, suggesting that the plasma membrane was the primary site of TBHP-induced damage. Overnight incubation of cultures with > or = 1 mumol/L dl-alpha-tocopherol protected cells against oxidant-induced changes. In parallel studies, overnight incubation of HepG2 in medium containing micelles with either BC or LUT (final concentrations of 1.1 and 10.9 mumol/L, respectively), the cell content of the carotenoids increased from < 0.04 to 0.32 and 3.39 nmol/mg protein, respectively. Carotenoid-loaded cells were partially or completely protected against oxidant-induced changes in lipid peroxidation, LDH release and amino acid and deoxyglucose transport. These data demonstrate that BC and LUT or their metabolites protect HepG2 cells against oxidant-induced damage and that the protective effect is independent of provitamin A activity.
authors.affiliation: Department of Food, Nutrition and Food Service Management, University of North Carolina at Greensboro 27412-5001, USA.
authors.forename: K R
authors.initials: KR
authors.lastname: Martin
authors.forename: M L
authors.initials: ML
authors.lastname: Failla
authors.forename: J C
authors.initials: JC
authors.lastname: Smith
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003847:Deoxyglucose / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014975:Lutein / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D008823:Micelles; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010545:Peroxides / Q000494:pharmacology; D010865:Pilot Projects; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D014316:Tritium; D014801:Vitamin A / Q000819:agonists; D014810:Vitamin E / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology; D019207:beta Carotene / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide
chemical_list: D000621:Aminoisobutyric Acids; D000975:Antioxidants; D002250:Carbon Radioisotopes; D008823:Micelles; D010545:Peroxides; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D014316:Tritium; D014801:Vitamin A; D014810:Vitamin E; D020122:tert-Butylhydroperoxide; D003847:Deoxyglucose; D007770:L-Lactate Dehydrogenase; D007930:Leucine; D014975:Lutein