Gene identification by chromosomal in-situ hybridization, microdissection and polymerase chain reaction amplification.
This paper describes a theoretical method by which candidate genes for inherited diseases can be identified by polymerase chain reaction amplification and subsequent cloning of organ-specific complementary deoxyribonucleic acid libraries hybridized in situ to, and subsequently dissected from, the cognate chromosome. A specific application of this technique to isolate genes related to genetic haemochromatosis is outlined.
Sallie
R
R
Liver Unit, National Institute for Health, Bethesda, Maryland 20892, USA. RichardS@BDG10.NIDDK.NIH.gov
D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006432:Hemochromatosis / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*