Electrochemical activity of 6-aminoquinolyl urea derivatives of amino acids and peptides. Application to high-performance liquid chromatography with electrochemical detection.
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (6-AQC) is a reagent used to increase the detection-sensitivity of amino acids and peptides in high-performance liquid chromatography with fluorescence detection. In this paper, the electrochemical characteristics of the derivatives of 6-AQC are described. Electrochemical detection of 6-AQC amino acids and peptide derivatives following reverse-phase HPLC are also reported. The response linearity of the derivatives on an amperometric detector was studied in the range of 5 pmol (0.5 mu M) to 2500 pmol (250 mu M). Approximately 2.5 pmol of the amino acid derivatives could be detected. The quantitative results of amino acids in plasma and a bovine serum albumin hydrolysate agreed well with values reported in the literature.
Li
G D
GD
Department of Chemistry, Northeastern University, Boston, MA 02115, USA.
Krull
I S
IS
Cohen
S A
SA
D000596:Amino Acids / Q000097:blood / Q000737:chemistry*; D000634:Aminoquinolines / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010455:Peptides / Q000097:blood / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014508:Urea / Q000737:chemistry*
D000596:Amino Acids; D000634:Aminoquinolines; D010455:Peptides; D012710:Serum Albumin, Bovine; D014508:Urea