The "missing" typical Rhizobium leguminosarum O antigen is attached to a fatty acylated glycerol in R. leguminosarum bv. trifolii 4S, a strain that also lacks the usual tetrasaccharide "core" component.
Rhizobium leguminosarum bv. trifolii 4S has a lipopolysaccharide O antigen that lacks galactose and many of the typical glycosyl components found in related strains. Here, we show that it also lacks the typical core tetrasaccharide but synthesizes an alternative glycolipid that contains galactose and the typical O-antigen glycosyl components, suggesting that in this strain, the O antigen is transferred to an alternative lipid acceptor.
Cedergren
R A
RA
Department of Biochemistry, Michigan State University, East Lansing 48824-1319, USA.
Wang
Y
Y
Hollingsworth
R I
RI
D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D016961:Rhizobium leguminosarum / Q000737:chemistry* / Q000276:immunology; D013045:Species Specificity
D006017:Glycolipids; D019081:O Antigens; D009844:Oligosaccharides