title: Eucalypt alpha-tubulin: cDNA cloning and increased level of transcripts in ectomycorrhizal root system.
abstract: Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (No. 270) of the ectomycorrhizal basidiomycete Pisolithus tinctorius caused no changes in root transcript levels of EgTubA1, whereas a drastic up-regulation in its expression was observed between 3 to 4 days after contact with the aggressive isolate 441. This enhanced alpha-tubulin expression coincided with the increase lateral root formation induced by fungal colonisation. The changes in alpha-tubulin expression support a role for cytoskeleton components in ectomycorrhiza development.
authors.affiliation: Equipe de Microbiologie Forestière, Centre de Recherches de Nancy, Institut National de la Recherche Agronomique, Champenoux, France.
authors.forename: E C
authors.initials: EC
authors.lastname: Diaz
authors.forename: F
authors.initials: F
authors.lastname: Martin
authors.forename: D
authors.initials: D
authors.lastname: Tagu
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000472:pathogenicity / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005052:Eucalyptus / Q000235:genetics / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis; D014158:Transcription, Genetic / Q000502:physiology; D014404:Tubulin / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D014404:Tubulin