Oxidant Production by Human B Lymphocytes: Detection of Activity and Identification of Components Involved
The O-2-generating NADPH oxidase, originally thought to be expressed only in phagocytic cells of the immune system, can also be expressed in B lymphocytes. Epstein-Barr virus-transformed B cell lines (EBV-BL) can generate O-2 at rates corresponding to between 1 and 5% of the rates obtained by activated neutrophils. The composition of the NADPH oxidase of EBV-BL appears to be identical to that of phagocytic cells. In this report, methods are described for the establishment of EBV-BL, together with assays for the measurement of reactive oxidant production and detection of the constituent components of the NADPH oxidase in these cells.
Jones
OTG
Department of Biochemistry, University of Bristol, Bristol, BS8 1TD, United Kingdom
Wood
JD