Fast desensitizing kainate-gated current resolved in whole-cell experiments on isolated rat hippocampal neurons.
In whole-cell concentration-jump experiments on dissociated rat hippocampal neurons the existence of a fast desensitizing component of a kainate-gated current was demonstrated. More than 90% of all neurons tested possessed such a receptor. Electrophysiological and pharmacological data suggest an association of this fast kainate-gated current with that mediated through a combination of subunits forming a low-affinity "(RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolopropionic acid (AMPA)-preferring' receptor. Kinetic data suggest the existence of a compact spatial location of fast desensitizing "AMPA-preferring' receptors which are naturally expressed on the surface of the neurons. An AMPA-insensitive current component mediated through fast desensitizing high-affinity kainate receptors was not observed.
Pidoplichko
V I
VI
Department of Neurophysiology, Federal Institute for Neurobiology, Magdeburg, Germany.
Wilsch
V W
VW
Reymann
K G
KG
D000818:Animals; D001581:Benzothiadiazines / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology*; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D018408:Patch-Clamp Techniques; D051381:Rats
D001581:Benzothiadiazines; D018690:Excitatory Amino Acid Agonists; D003208:Concanavalin A; C004639:cyclothiazide; D007608:Kainic Acid