title: Continuous Cell Line from Pupal Ovary of Perina nuda (Lepidoptera: Lymantriidae) That Is Permissive to Nuclear Polyhedrosis Virus from P. nuda
abstract: A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28&deg;C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).
authors.affiliation: Department of Plant Pathology and Entomology, National Taiwan University, Taipei, Taiwan, ROC
authors.initials: CH
authors.lastname: Wang
authors.initials: CM
authors.lastname: Chou
authors.initials: HC
authors.lastname: Liu
authors.initials: SL
authors.lastname: Kau
authors.initials: GH
authors.lastname: Kou
authors.initials: CF
authors.lastname: Lo