Demonstration and definition of the noncovalent binding selectivity of agents related to CC-1065 by an affinity cleavage agent: noncovalent binding coincidental with alkylation.
A study of the DNA cleavage efficiency and selectivity of CDPI3-EDTA (4), an affinity cleavage agent based on the structure of CC-1065, is described. The studies with 4 provide direct evidence of AT-rich noncovalent binding coincidental with all DNA alkylation sites observed with (+)- or ent-(-)-CC-1065.
Boger
D L
DL
Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA.
Zhou
J
J
Cai
H
H
D000478:Alkylation; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism; D000080890:Duocarmycins; D007211:Indoles; D007933:Leucomycins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D013237:Stereoisomerism
D000903:Antibiotics, Antineoplastic; D004279:DNA, Viral; D000080890:Duocarmycins; D007211:Indoles; D007933:Leucomycins; C018782:CC 1065