title: Effects of vitamin E deficiency and dietary linoleate on serum thromboxane synthesis in male sprague-dawley rats.
abstract: The aim of the present study was to examine whether vitamin E deficiency and dietary linoleate had additive or synergistic effects on serum thromboxane (TX) status and therefore on thrombogenesis. Eight groups of five male weaning Sprague-Dawley rats were fed semipurified diets containing 3.5 or 18.4% of energy from linoleate (en% linoleate) and 0, 100, 5000, 15,000 ppm vitamin E for 8 weeks. Rats fed no vitamin E had the lowest serum vitamin E while rats fed 15,000 ppm vitamin E had the highest serum vitamin E (p < 0.05). Serum 18:2, n-6 (linoleic acid; LA) and 20:4, n-6 (arachidonic acid; AA) were significantly greater in the 18.4 en% linoleate group than in the 3.5 en% linoleate group (p < 0.05). Serum TXA2, measured as its stable metabolite TXB2, was significantly greater in the vitamin E-deficient rats than in the vitamin E-adequate and vitamin E-supplemented rats (p < 0.05). Serum lipid peroxidation, measured as thiobarbituric acid reactive substances (TBARS), was significantly greater in the 0 and 100 ppm vitamin E groups than in the 5000 and 15,000 ppm vitamin E groups (p < 0.05). No interaction between dietary linoleate and vitamin E deficiency on serum TX status was found. However, it seemed that vitamin E deficiency had a more potent effect on TX synthesis than dietary linoleate. The result suggested that vitamin E deficiency may be prothrombogenic via its effect on TX synthesis.
authors.affiliation: Department of Nutrition, Chung Shan Medical College, Taiwan.
authors.forename: H W
authors.initials: HW
authors.lastname: Chen
authors.forename: C D
authors.initials: CD
authors.lastname: Lii
authors.forename: M L
authors.initials: ML
authors.lastname: Wang
authors.forename: C C
authors.initials: CC
authors.lastname: Ou
authors.forename: M F
authors.initials: MF
authors.lastname: Wang
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage* / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013929:Thromboxane B2 / Q000097:blood*; D014810:Vitamin E / Q000008:administration & dosage; D014811:Vitamin E Deficiency / Q000097:blood*
chemical_list: D004041:Dietary Fats; D008041:Linoleic Acids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D016718:Arachidonic Acid; D013929:Thromboxane B2; D019787:Linoleic Acid