Localization of prolidase gene expression in scar tissue using in situ hybridization.
We investigated prolidase gene expression in human skin by means of Northern blot analysis and in situ hybridization. Northern blot analysis revealed that an mRNA species that was specific for prolidase was present in cultured human skin fibroblasts and keratinocytes. In situ hybridization using non-isotopic riboprobes labeled with digoxigenin and an isotopic riboprobe labeled with [35S]UTP localized prolidase gene expression to fibroblasts and endothelial cells of small vessels in scar tissue. Prolidase mRNA was also prominently expressed in keratinocytes near the basal layer overlying scar tissue. These findings indicate that prolidase may have an important role in wound healing.
Senboshi
Y
Y
Department of Dermatology, Okayama University Medical School, Japan.
Oono
T
T
Arata
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D002648:Child; D002921:Cicatrix / Q000235:genetics* / Q000473:pathology; D004150:Dipeptidases / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution
D012333:RNA, Messenger; D004150:Dipeptidases; C022449:proline dipeptidase