Sodium channel mRNA in the B104 neuroblastoma cell line.
B104 neuroblastoma cells are excitable, but the ion channels underlying electrogenesis in these cells have not been identified. RT-PCR, restriction enzyme analysis and in situ hybridization were used to study sodium channel mRNAs in B104 cells. High levels of sodium channel alpha-subunit mRNAs III, NaG and Na6 and beta 1-subunit mRNA were detected by RT-PCR in B104 cells. Low levels of types I and II alpha-subunit mRNAs were also present. In situ hybridization with subtype-specific riboprobes detected sodium channel alpha-subunit mRNAs III, NaG and Na6 and beta 1-subunit mRNA in B104 cells; analysis of the percentage of B104 cells expressing each alpha-subunit mRNA subtype suggests that some cells express the mRNAs for several alpha-subunits.
Dib-Hajj
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Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, USA.
Hinson
A W
AW
Black
J A
JA
Waxman
S G
SG
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D017403:In Situ Hybridization; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D015183:Restriction Mapping; D015222:Sodium Channels / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D046911:Macromolecular Substances; D012333:RNA, Messenger; D015222:Sodium Channels