title: Biochemical and biomechanical alterations in equine articular cartilage following an experimentally-induced synovitis.
abstract: The effects of inflammation on the biochemical and biomechanical properties of articular cartilage at two sites (dorsal and palmar) from the radial facet of the equine third carpal bone were examined in response to a synovitis induced with Escherichia coli lipopolysaccharide (LPS). Four groups were studied. In group 1 synovitis was induced at time zero and evaluated at week 6. Group 2 was the sham-treated control for group 1. In group 3 synovitis was induced at time zero and evaluated at week 2. Group 4 was the sham-treated control for group 3. There was a significant increase (P < 0.05) in newly synthesized proteoglycan PG from both sites in group 3 as compared to the sham-treated groups and group 1. No significant difference in the endogenous PG concentration between groups or sites was detected. Sepharose CL-2B revealed two peaks of newly synthesized PG in all groups; an early peak (Kav 0.11-0.13) and a late peak (Kav 0.48-0.64). Newly synthesized PG profiles from sham-treated groups and group 3 were similar, but the group 3 PG profile exhibited a more pronounced early peak. Conversely, the PG profile from group 1 demonstrated a more prominent late peak. Electrophoresis and Western blot analysis of the pooled late PG peak fractions from the sham-treated and group 1 showed a single toluidine blue stained band from both sites which reacted with monoclonal antibody (MAb) 1C6. By contrast, the late peak from the palmar site in group 3 showed an additional faster moving component on composite gels which did not react with MAb 1C6. There was a significant decrease in Poisson's ratio and a significant increase in cartilage thickness in groups 1 and 3 which had received synovitis. The increase in cartilage thickness of groups 1 and 3 was also significantly affected by site (dorsal > palmar). There was no significant difference in aggregate modulus or permeability constant among groups. Primary joint inflammation induced by LPS alters the biochemical and biomechanical properties of the articular cartilage as a function of time and site. An increase in chondrocyte PG synthesis in the early period following synovitis may be a reparative response to the inflammatory insult. Continued alterations in the qualitative PG composition in the later period following synovitis may represent a shift in chondrocyte metabolism to repopulate the existing cartilage matrix.
authors.affiliation: Department of Veterinary Clinical Sciences, Ohio State University, Colombus, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Palmer
authors.forename: A L
authors.initials: AL
authors.lastname: Bertone
authors.forename: C J
authors.initials: CJ
authors.lastname: Malemud
authors.forename: J
authors.initials: J
authors.lastname: Mansour
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D006736:Horses; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*
chemical_list: D011509:Proteoglycans