Binding of interferon-alpha and -beta to a component of the human type I interferon receptor expressed in simian cells.
The type I interferons (IFNs) are a family of homologous cytokines that compete for receptor binding. Past experiments with a cloned human IFN-alpha receptor component (designated herein as HuIFNAR-1) transfected into different cell backgrounds have given contradictory results in terms of binding and signalling after exposure of cells to different human type I IFNs. In order to investigate the binding specificity of human type I IFN subtypes to HuIFNAR-1, a cDNA encoding HuIFNAR-1 was transfected into simian COS cells. HuIFNAR-1 expression in COS cells, which was confirmed by Northern blot analysis, resulted in increased binding of 125I-labelled HuIFN-alpha 2 and -beta. These data support the participation of this receptor component in ligand binding, probably in association with other receptor components.
Hwang
S Y
SY
Molecular Genetics and Development Group, Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia.
Holland
K A
KA
Kola
I
I
Hertzog
P J
PJ
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D003001:Cloning, Molecular; D018014:Gene Transfer Techniques; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism*; D011869:Radioligand Assay; D017471:Receptors, Interferon / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D016899:Interferon-beta