Two nuclear proteins bind to the promoter P3 region of the human parathyroid hormone-related peptide gene.
We have used a 5' regulatory sequence fragment of the human parathyroid hormone-related peptide (PTHrP) gene to identify nuclear DNA-binding proteins (DBP), using South-Western analysis. The PTHrP 549 bp DNA fragment was amplified from a human genomic DNA, and composed of 5' non-coding exon Ic, the intervening intron and 5' non-coding exon II. The DNA fragment bound very specifically to a 70 kDa and a 65 kD protein from the nuclear extract of human hepatocyte, HepG2, and keratinocyte, SVK-14, cell lines. This is the first evidence of a physical binding between nuclear protein and the human PTHrP gene. The 70 kDa and 65 kDa nuclear proteins may have a role in the regulation of human PTHrP gene expression.
Bui
T D
TD
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London.
Woe
H Y
HY
Khan
I
I
Burton
P B
PB
Moniz
C F
CF
D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000235:genetics* / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C470121:PTHLH protein, human; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins