title: High performance thin layer chromatographic analysis of hydrolyzed tinidazole solutions. I. Development and validation method.
abstract: A stability-indicating high performance thin layer chromatography method for analyzing hydrolyzed tinidazole solutions using silica gel plates was developed and validated. The mobile phase used was methanol-diethyl ether-chloroform (1:9:3, v/v/v) allowing small changes in its composition. Detection was at 314 mm. Rf values being 0.1-0.4, baseline resolution was achieved for tinidazole and the hydrolysis products. The analytes were stable on the sorbent and could be precisely and accurately measured in the range 20-170 ng per band.
authors.affiliation: Department of Pharmacy, University of Helsinki, Finland.
authors.forename: J P
authors.initials: JP
authors.lastname: Salo
authors.forename: H
authors.initials: H
authors.lastname: Salomies
mesh_terms: D000994:Antitrichomonal Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry; D004355:Drug Stability; D006868:Hydrolysis; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014011:Tinidazole / Q000032:analysis*
chemical_list: D000994:Antitrichomonal Agents; D012996:Solutions; D014011:Tinidazole