Morphological analysis of yeast cells using an automated image processing system.
In order to control the growth of the yeast Saccharomyces cerevisiae during cultivations, a fully-automated sampling and analysing system has been developed. Based on the microscopic appearance of cells, a detailed characterization of yeast suspensions from the bioreactor is available, presenting data of current cell concentrations, distributions of process-dependent cell structures and parts of cells containing vacuoles. This measuring system combines quantitative and qualitative analysis methods in a digital image processing technique, which offers the possibility of on-line estimation of cell development that approximates real-time. Perturbations within the process are detectable in time and regulative actions can be done without any delay.
Zalewski
K
K
Institute of Biotechnology, Technical University Berlin, Germany.
Buchholz
R
R
D019149:Bioreactors; D001709:Biotechnology; D002452:Cell Count; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development; D014617:Vacuoles / Q000648:ultrastructure