Stability and stabilization of Fusobacterium necrophorum hemolysin.
The stability and stabilization of the hemolytic activity of Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme were monitored over a period of four weeks using culture supernatants. The hemolytic activity was completely lost after one week at room temperature and 37 degrees C. After a two-week storage at 4 degrees C and -80 degrees C only trace activity was detected with -80 degrees C being the better of the two conditions. The addition of cysteine monohydrochloride, bovine serum albumin or Tween 80 as stabilizers, however, led to the detection of a considerable amount of the hemolytic activity in the sample stored at 4 degrees C and - 80 degrees C throughout the period investigated. The hemolytic activity appeared to be more stable in the presence of Tween 80 at -80 degrees C. Cysteine monohydrochloride was found to crystallize at - 80 degrees C and was therefore ineffective as a stabilizer at this temperature. Hemoglobin was also ineffective as a stabilizer.
Amoako
K K
KK
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
Goto
Y
Y
Misawa
N
N
Xu
D L
DL
Shinjo
T
T
D000418:Albumins / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D005079:Excipients / Q000494:pharmacology*; D005675:Fusobacterium necrophorum / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000187:drug effects* / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology*; D013696:Temperature
D000418:Albumins; D005079:Excipients; D006460:Hemolysin Proteins; D011136:Polysorbates; D003545:Cysteine