Nucleic acid binding by zinc finger-like motif of human papillomavirus type 16 E7 oncoprotein.
Human papillomavirus (HPV) types 16 and 6b E7 proteins and their chimeric or mutant proteins were analyzed for oligonucleotide-binding activity by surface plasmon resonance-based biomolecular interaction analysis. The results indicated that type 16 E7 protein has stronger nucleic acid-binding activity than that of type 6b E7 protein. In addition, the results also indicated that the zinc finger-like motif in the C-terminal region of the type 16 E7 protein plays an important role in this activity.
Chinami
M
M
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
Inoue
M
M
Masunaga
K
K
Fukuma
T
T
Shingu
M
M
Toyoda
T
T
D001665:Binding Sites; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers
C085208:E7 protein, Human papillomavirus 6b; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; C059731:oncogene protein E7, Human papillomavirus type 16