A new method for permeabilization of the plasma membrane of cultured mammalian cells. II. Mechanism for potentiation of bleomycin cytotoxicity by vortex-stirring with high molecular weight polyacrylic acid.
As previously reported, the cytotoxicity of bleomycin (BLM) toward cultured mammalian cells was remarkably potentiated by vortex-stirring this agent with the cells for a few seconds in the presence of high molecular weight polyacrylic acid (PAA). This paper describes that the enhancement of BLM cytotoxicity was also achieved by a sequential BLM-treatment immediately after vortex-stirring cells with PAA only. It was suggested that this enhancement resulted from the transient permeabilization of the plasma membrane induced during vortex-stirring in the presence of PAA, leading to an increase in BLM incorporation. This mechanism was supported by the finding of an appreciable uptake by vortex-stirred cells of Lucifer Yellow, a known non-permeant material. This procedure, which did not affect any cell viability, might be a beneficial method for transient permeabilization of cultured mammalian cells to facilitate the internalization of non-permeant materials.
Shimizu
N
N
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Kawazoe
Y
Y
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D001761:Bleomycin / Q000378:metabolism*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002470:Cell Survival; D005456:Fluorescent Dyes; D007546:Isoquinolines; D007939:Leukemia L1210; D051379:Mice; D013696:Temperature; D013997:Time Factors
D000180:Acrylic Resins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D001761:Bleomycin; C006903:carbopol 940; C017475:lucifer yellow