Photobiological properties of a new tetramethylfuroquinolinone.
1,4,6,8-tetramethyl-2H-furo[2,3-h]quinolin-2-one (FQ) is a new isoster of angelicin characterised by an extremely strong photosensitizing activity, which is several times higher than that of 8-MOP and 4,6,4'-trimethylangelicin (TMA). Following treatment with 1.2 microM FQ and a dose as low as 0.05 kJ m(-2) of UVA irradiation, survival (colony forming ability) of HeLa cells was abolished, while TMA and 8-MOP (even at five times the concentration for the latter) were practically ineffective. Upon UVA irradiation FQ induces various types of lesions in mammalian cells in DNA: single-strand breaks (SSBs), many monoadducts and covalent DNA-protein cross-links (DPC), but not interstrand cross-links (ISC). Using the two step irradiation procedure, DPC induced by FQ appeared to be severe lesions, having a high antiproliferative activity; their formation requires the successive absorption of two photons, thus, in this respect, resembling ISC formation. In spite of its higher capacity for damaging DNA, FQ showed a skin-phototoxicity potency very similar to 8-MOP. As some benzopsoralens, FQ induced a certain antiproliferative activity also in the dark, which was accompanied by the formation of double-strand breaks into DNA associated with DPC. This lesion is generally induced by topoisomerase inhibitors. On the basis of these features, FQ can be expected to show useful activities in photochemotherapy and photopheresis. However, before medical use careful studies on its genotoxicity are required.
Bordin
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F
Department of Pharmaceutical Chemistry of Padua University, CNR, Padova, Italy. BORDINPDFARM.DSFARM.UNIPD.IT
Marzano
C
C
Carlassare
F
F
Rodighiero
P
P
Guiotto
A
A
Caffieri
S
S
Baccichetti
F
F
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002455:Cell Division; D003432:Cross-Linking Reagents; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D011564:Furocoumarins / Q000737:chemistry / Q000494:pharmacology; D006168:Guinea Pigs; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D008730:Methoxsalen / Q000737:chemistry / Q000494:pharmacology; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D015363:Quinolones / Q000737:chemistry / Q000494:pharmacology*; D013053:Spectrophotometry; D014466:Ultraviolet Rays
C101951:1,4,6,8-tetramethyl-2H-furo(2,3-h)quinolin-2-one; D003432:Cross-Linking Reagents; D011564:Furocoumarins; D017319:Photosensitizing Agents; D015363:Quinolones; D004247:DNA; C011659:angelicin; D008730:Methoxsalen