A transposon insertion in the Arabidopsis SSR16 gene causes an embryo-defective lethal mutation.
The SSR16 gene of Arabidopsis has been identified as a gene encoding a ribosomal protein S16 homolog through analysis of a transposon insertion mutation. The insertion mutation is lethal, arresting embryonic development at approximately the transition from the globular to the heart stage of embryonic development. Co-segregation of the mutant phenotype with the transposon-borne drug-resistance marker and loss of the inserted transposon concomitant with phenotypic reversion provided evidence that the transposon had caused the mutation. Sequences flanking the insertion site were amplified from DNA of viable heterozygotes by thermal asymmetric interlaced (TAIL) PCR. The amplified fragment flanking the 3' end of the inserted element was sequenced and found to be identical to an Arabidopsis expressed sequence tag (EST). The EST, in turn, contained a coding sequence homologous to the ribosomal protein S16 (RPS16) of bacteria such as Escherichia coli, Bacillus subtilis and Salmonella typhimurium, as well as Neurospora crassa mitochondria and higher plant plastids. Thus the gene identified by the embryo-defective lethal insertion mutation encodes an RPS16 homolog and has been designated the SSR16 gene.
Tsugeki
R
R
Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA.
Kochieva
E Z
EZ
Fedoroff
N V
NV
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D002934:Cinnamates; D004251:DNA Transposable Elements / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004351:Drug Resistance; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D005804:Genes, Lethal / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D012269:Ribosomal Proteins / Q000235:genetics*; D012639:Seeds / Q000196:embryology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D002934:Cinnamates; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D012269:Ribosomal Proteins; C023509:ribosomal protein S16; D006921:Hygromycin B; C026273:hygromycin A