Characterisation of a human chromosome 1 somatic cell hybrid mapping panel and regional assignment of 6 novel STS.
A somatic cell hybrid mapping panel has been constructed which allows subdivision of human chromosome 1 into 8 distinct subregions. All of the hybrids carry copies of chromosome 1 with specific deletions and the position of the breakpoints has been determined relative to the location of microsatellite markers in the genetic linkage map produced by Genethon. The majority of the breakpoints can be positioned between adjacent loci on the map. The usefulness of this hybrid panel for physical mapping has been demonstrated by the regional assignment of 6 novel STS markers made from Alu-PCR clones generated from a hybrid which contains the short arm of chromosome 1.
Roberts
T
T
Department of Neurosciences, Cleveland Clinic Foundation, Ohio 44195, USA.
Mead
R S
RS
Cowell
J K
JK
D019457:Chromosome Breakage / Q000235:genetics; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017421:Sequence Analysis; D016324:Sequence Tagged Sites