title: Pharmacological profiling of recombinant tissue factor pathway inhibitor.
abstract: Tissue factor pathway inhibitor (TFPI) is a Kunitz-type serine protease inhibitor found within the vascular system that is known to be released upon heparin administration. Tissue factor pathway inhibitor regulates the extrinsic pathway by inhibiting both factors VIIa and Xa. The role of TFPI in the prevention of thrombosis is currently being elucidated. These studies describe the pharmacological profile of recombinant variants of this inhibitor that have recently been made available for study. Recombinant TFPI (rTFPI) exhibits potent anticoagulant (PT) and antiprotease actions (anti-Xa). While carboxy truncated forms of the inhibitor were not observed to prolong the PT, potent anti-Xa effects were still noted. In a rabbit model of stasis-thrombosis, full-length rTFPI dose-dependently inhibited thrombus formation, producing a complete inhibition of thrombosis at a dose of 500 micrograms/kg. At doses that were antithrombotically effective, minor increases in blood loss were observed in a rabbit ear bleeding model. The effect of TFPI on the inhibition of platelet activation was assessed using a flow cytometric assay. Three hundred nmol/L of rTFPI were observed to block the activation of platelets by 4.5 pmol/L recombinant tissue factor. In addition to participating in the maintenance of hemostasis, rTFPI may prove to be an effective therapeutic modality for preventing thrombus formation.
authors.affiliation: Cardiovascular Institute, Loyola University Medical Center, Maywood, IL 60153, USA.
authors.forename: W
authors.initials: W
authors.lastname: Jeske
authors.forename: D
authors.initials: D
authors.lastname: Hoppensteadt
authors.forename: D
authors.initials: D
authors.lastname: Callas
authors.forename: M J
authors.initials: MJ
authors.lastname: Koza
authors.forename: J
authors.initials: J
authors.lastname: Fareed
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011517:Prothrombin Time; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D065427:Factor Xa Inhibitors; D008074:Lipoproteins; D011994:Recombinant Proteins; C051928:lipoprotein-associated coagulation inhibitor; D006493:Heparin