Forces on chromosomal DNA during anaphase.
In the course of anaphase, the chromosomal DNA is submitted to the traction of the spindle. Several physical problems are associated with this action. In particular, the sister chromatids are generally topologically intertwined at the onset of anaphase, and the removal of the intertwinings results from a coupling between the enzymatic action of type II DNA topoisomerases and the force exerted by the spindle. We propose a physical analysis of some of these problems: 1) We compare the maximum force the spindle can produce with the force required to break a DNA molecule, and define the conditions compatible with biological safety during anaphase. 2) We show that the behavior of the sister chromatids in the absence of type II DNA topoisomerases can be described by two distinct models: a chain pullout model accounts for the experimental observations made in the budding yeast, and a model of the mechanical rupture of rubbers accounts for the nondisjunction in standard cases. 3) Using the fluctuation-dissipation theorem, we introduce an effective protein friction associated with the strand-passing activity of type II DNA topoisomerases. We show that this friction can be used to describe the situation in which one chromosome passes entirely through another one. Possible experiments that could test these theoretical analyses are discussed.
Jannink
G
G
Laboratoire Léon Brillouin (CEA-CNRS), Departement de Biologie Cellulaire et Moléculaire, CEA/Saclay, Gif-sur-Yvette, France.
Duplantier
B
B
Sikorav
J L
JL
D000705:Anaphase / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001696:Biomechanical Phenomena; D055592:Biophysical Phenomena; D001703:Biophysics; D002875:Chromosomes / Q000502:physiology*; D004247:DNA / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000502:physiology; D012854:Sister Chromatid Exchange / Q000502:physiology; D008941:Spindle Apparatus / Q000502:physiology
D004247:DNA; D004250:DNA Topoisomerases, Type II