Isolation and characterization of a Pseudomonas aeruginosa genomic DNA sequence, encoding a putative DNA helicase belonging to superfamily I.
The isolation and characterization of a 2,122 bp DNA fragment from a Pseudomonas aeruginosa genomic library containing a 1,495 bp open reading frame (ORF) encoding a putative DNA helicase is described. The deduced amino acid (498 residues) sequence derived from this ORF, exhibited a high degree of homology to the rep and uvrD helicases of Escherichia coli (55 and 38% identities, respectively), and to those of Staphylococcus aureus and Haemophilus influenzae (43 and 39% identities, respectively), all of them belonging to superfamily I.
Avichezer
D
D
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Gilboa-Garber
N
N
Katcoff
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D004265:DNA Helicases / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D004265:DNA Helicases