Rapid detection of mutations associated with macrolide resistance in Mycobacterium avium complex.
Macrolide resistance in Mycobacterium avium can be detected with an adaption of a commercially available RNA/RNA duplex mismatch assay (Ambion, Austin, Tex.). The sensitivity and specificity values for the assay were 100% when evaluated against 41 macrolide-resistant and -susceptible strains of M. avium. Resistant subpopulations of approximately 20% could be readily detected. The assay is simple to perform and interpret, inexpensive, and rapid (< 24-h turnaround).
Nash
K A
KA
Department of Pathology and Laboratory Medicine, Childrens Hospital Los Angeles, CA 90027, USA. kanash@hsc.usc.edu
Inderlied
C B
CB
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D004252:DNA Mutational Analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D015269:Mycobacterium avium Complex / Q000187:drug effects*; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D017963:Azithromycin; D017291:Clarithromycin