title: Diversity of rotavirus strains infecting pediatric patients in New Delhi, India.
abstract: Polyacrylamide Gel Electrophoresis (PAGE) of rotavirus can provide information on variation in rotavirus strain prevalent in the community. In the present study 157 samples were collected from children below 5 years of age presenting with acute diarrhoea from May to December, 1990 at Safdarjung Hospital, New Delhi. Seventy-one (45 percent) of these stool samples were positive for rotavirus by ELISA. Sixty-seven samples showed discernible RNA pattern of group A rotavirus by PAGE. It was found that there were seven electropherotypes co-circulating in this 8-month period. Majority of the strains had a IIC pattern, but IIA, IID, IIE, IIG, IE, and IB electropherotype patterns were also seen. The simultaneous co-circulation of multiple group A strains in the community may lead to extensive genomic variation in rotavirus strains.
authors.affiliation: Department of Microbiology, All India Institute of Medical Science, New Delhi, India.
authors.forename: B
authors.initials: B
authors.lastname: Chatterjee
authors.forename: M
authors.initials: M
authors.lastname: Husain
authors.lastname: Kavita
authors.forename: P
authors.initials: P
authors.lastname: Seth
authors.forename: S
authors.initials: S
authors.lastname: Broor
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005759:Gastroenteritis / Q000821:virology*; D014644:Genetic Variation; D006801:Humans; D007194:India; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000150:complications* / Q000821:virology*; D012703:Serotyping; D014504:Urban Health