A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction.
A polymerase chain reaction (PCR) method was developed for detection of salmonella in food. A set of PCR primers was designed to amplify a 199 bp salmonella-specific DNA fragment derived from a repetitive DNA of Salmonella Weltevreden. The assay detected all 52 most prevalent serovars found in contaminated food in Thailand and no cross-reaction was observed with other non-salmonella organisms. The limit of detection was 1 fg of purified target DNA or five bacteria from pure culture. The detection of artificially contaminated food performed following a 6 h enrichment step was three bacteria per gram and the result was obtained within 4 h.
Jitrapakdee
S
S
Department of Biochemistry, Mahidol University, Bangkok, Thailand.
Tassanakajon
A
A
Boonsaeng
V
V
Piankijagum
S
S
Panyim
S
S
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005628:Frozen Foods / Q000382:microbiology; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000517:prevention & control*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004269:DNA, Bacterial