title: Isolation of haemorphin-related peptides from filter membranes collected in connection with haemofiltration of human subjects.
abstract: This paper describes the extraction and isolation from dialysis filters of two peptides containing the opioid active sequence haemorphin-7. The filter devices were obtained from uraemic patients subjected to haemofiltration. Following acidic extraction of the filter membranes the peptides were purified by size-exclusion, ion-exchange chromatography and finally by reversed-phase chromatography using different columns and different chromatographic systems. The purification was guided by radioimmunoassay and the structure of the final products was elucidated by N-terminal sequencing and fast-atom bombardment mass spectrometry as well as micro-electrospray mass spectrometry. The isolated peptides were suggested to be identical to fragments 1-41 and 32-41 of the beta-chain of human haemoglobin.
authors.affiliation: Department of Internal Medicine, Uppsala University Hospital, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Nyberg
authors.forename: K
authors.initials: K
authors.lastname: Sanderson
authors.forename: P
authors.initials: P
authors.lastname: Andren
authors.forename: M
authors.initials: M
authors.lastname: Thornwall
authors.forename: M
authors.initials: M
authors.lastname: Einarsson
authors.forename: B
authors.initials: B
authors.lastname: Danielson
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000595:Amino Acid Sequence; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005914:Globins / Q000737:chemistry; D006440:Hemofiltration / Q000295:instrumentation*; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D018847:Opioid Peptides / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D017421:Sequence Analysis; D017385:Sequence Homology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014511:Uremia / Q000628:therapy
chemical_list: D006454:Hemoglobins; D018847:Opioid Peptides; D010446:Peptide Fragments; C085122:hemorphin 7; D005914:Globins