Alzheimer's presenilin 1 gene expression in platelets and megakaryocytes. Identification of a novel splice variant.
The presenilin 1 (PS1) gene located on chromosome 14 has been linked with the majority of early-onset FAD. The normal biological role of PS1 as well as the mechanism by which mutations in PS1 cause FAD remains unknown. PS1 expression in platelets and the Dami megakaryocytic cell line was examined by Western blot analysis and RT-PCR. Using an anti-N-terminus PS1 antibody we detected PS1 immunoreactive bands of 44, 32 and 27 kDa in both cell types. After RT-PCR we observed that platelets and megakaryocytes carry at least four different PS1 transcripts. One of them is a novel PS1 splice variant that lacks the coding sequence for exon 10 resulting in a shorter 409 amino acid protein.
Vidal
R
R
Department of Pathology, New York University Medical Center, NY 10016, USA. Ruben.Vidal@MCFPO.med.nyu.edu
Ghiso
J
J
Wisniewski
T
T
Frangione
B
B
D017398:Alternative Splicing; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D005091:Exons; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D053764:Presenilin-1; D012313:RNA / Q000378:metabolism
D006684:HLA-DR Antigens; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D012313:RNA