title: alpha-subunit of CaM-KII increases glycine currents in acutely isolated rat spinal neurons.
abstract: 1. Here we report that in acutely isolated rat spinal dorsal horn neurons, the glycine receptor can be regulated by exogenous calcium/ calmodulin-dependent protein kinase II (CaM-KII). Intracellularly applied, the alpha-subunit of CaM-KII enhanced glycine receptor-activated current recorded with the use of the whole cell patch-clamp technique. This result suggests that the function of glycine receptor is modulated by CaM-KII, but the cellular mechanism underlying the enhancement of glycine receptor-activated current is still unknown.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames 50011, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Randić
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000494:pharmacology*; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018009:Receptors, Glycine / Q000187:drug effects*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018118:Chloride Channels; D018009:Receptors, Glycine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005998:Glycine