A novel plasmid gene involved in bacteriophage PRD1 infection and conjugative host-range.
PRD1 infects bacteria carrying IncN plasmids by binding to their conjugative pili. Mutations in a plasmid locus kikA close to the pilus region result in PRD1 resistance and reduced conjugation proficiency to Klebsiella but not to Escherichia coli. One of the two genes of kikA is sufficient to restore both normal phenotypes. PRD1 binds to cells carrying the mutant plasmid but fails to inject its genome.
Holcík
M
M
Department of Biology, Carleton University, Ottawa, Ontario, Canada. mholcik@hhmivax.humgen.upenn.edu
Iyer
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VN
D001435:Bacteriophages / Q000472:pathogenicity*; D007709:Klebsiella / Q000235:genetics*; D010957:Plasmids