Chemometric methods applied to an ICP-AES study of chemical element distributions in autopsy livers from subjects affected by Wilson and beta-thalassemia.
The concentrations of seven elements (Ca, Cu, Fe, Mg, P, S and Zn) in three autopsy livers (from two beta-thalassemic patients and one Wilson's disease patient) were determined by ICP-AES technique. At autopsy the three livers were subdivided into a large number of samples for a detailed study of the distribution of Fe and Cu, the accumulation of which characterizes the two diseases. In the same samples Ca, Mg, P, S and Zn concentrations were also determined in order to study significant variations or anomalous trends that could help identify these diseases. Our results generally show a good coincidence with literature data within the limits of sample variability. Based on Factor Analysis as well as Regression Analysis there is evidence of a high correlation between Fe and P contents in beta-thalassemia. The latter finding led us to propose tentatively an accumulation of Fe as a complex with P-containing molecules.
Aragoni
M C
MC
Dipartimento di Chimica e Tecnologie Inorganiche e Metallorganiche, Università di Cagliari, Italy.
Crisponi
G
G
Nurchi
V M
VM
Silvagni
R
R
Sciot
R
R
Ambu
R
R
Costa
V
V
Faa
G
G
D000328:Adult; D001344:Autopsy; D002118:Calcium / Q000032:analysis; D003300:Copper / Q000032:analysis; D004602:Elements; D006527:Hepatolenticular Degeneration / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D008274:Magnesium / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods*; D013455:Sulfur / Q000032:analysis; D015032:Zinc / Q000032:analysis; D017086:beta-Thalassemia / Q000378:metabolism*
D004602:Elements; D010758:Phosphorus; D013455:Sulfur; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium