Analysis of sodium dodecyl sulfate-stable cell wall aminopeptidases in strains of viridans streptococci.
Cell wall extracts from nine strains of viridans streptococci representing five species were analyzed for aminopeptidases. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains, all having enzyme bands at 89 and 45 kDa. Three strains had an additional band at 200 kDa. Crossreactivity between aminopeptidases of all active strains was shown with crossed immunoelectrophoresis. Strains of Streptococcus mutans and S. sobrinus were without detectable cell wall aminopeptidases.
Linder
L E
LE
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge Hospital, Sweden.
Lönnies
H
H
Sund
M L
ML
D000626:Aminopeptidases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002473:Cell Wall / Q000201:enzymology; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D009055:Mouth / Q000382:microbiology; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification / Q000201:enzymology*
D012967:Sodium Dodecyl Sulfate; D000626:Aminopeptidases