title: Vacuolating cytotoxic activity of Pasteurella multocida causing haemorrhagic septicaemia in buffalo and cattle.
abstract: The toxic activity of Pasteurella multocida strains which cause haemorrhagic septicaemia (HS) in buffalo and cattle was examined in a mouse model. Mice were injected intraperitoneally with 10(2) cells of P. multocida serotype B:2,5. Electron microscopy of peritoneal macrophages obtained 6 h after injection revealed strong induction of cytoplasmic vacuolation, macrophage lysis and death. In vitro experiments with the mouse macrophage cell line RAW 264 incubated with cultures of various HS- and non-HS-associated strains of P. multocida or with culture supernatants revealed macrophage vacuolation when HS-associated strains were used. On pre-incubation of the strains with antiserum obtained from buffalo infected with P. multocida serotype B:2,5 no vacuolation was observed. These results are indicative of the presence of vacuolating cytotoxic activity in HS-associated strains of P. multocida.
authors.affiliation: Center of Animal Biotechnology, Veterinary Research Institute, Peshawar, Pakistan.
authors.forename: N H
authors.initials: NH
authors.lastname: Shah
authors.forename: J
authors.initials: J
authors.lastname: Biewenga
authors.forename: N H
authors.initials: NH
authors.lastname: Shah
authors.forename: F K
authors.initials: FK
authors.lastname: de Graaf
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology* / Q000473:pathology; D003603:Cytotoxins / Q000633:toxicity*; D004195:Disease Models, Animal; D006483:Hemorrhagic Septicemia / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D008264:Macrophages / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D016979:Pasteurella multocida / Q000472:pathogenicity*; D014617:Vacuoles / Q000648:ultrastructure; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins