Influence of mouse placental factors on in vitro antibody synthesis.
Placental culture supernatants (PS) obtained from various mouse crossbreedings were added to mouse IgG1 hybridoma cultures producing anti-DNP antibodies. The quantity of monoclonal antibody (mAb) produced, the nature of these antibodies and the proliferation of the hybridoma cells were studied. It was observed that the supernatants increased or diminished the production of mAb, depending on the genetic origin of the placentae. This effect was the same using placentae from primiparous or multiparous females and it was not due to modifications of the cellular proliferation of the hybridoma, as shown by 3H-thymidine uptake. It was also found that placental supernatants induced an increase in the proportion of asymmetric, blocking antibodies synthesized by the hybridoma. This effect was detected with supernatants from both allogeneic or syngeneic crossbreedings, but only when placentae were obtained from multiparous females. These observations indicate that placentae produce at least two soluble factors that participate in the regulation of antibody synthesis and suggest that these factors play an important role in the immune equilibrium between mother and fetus.
Miranda
S
S
Instituto de Estudios de la Immunidad Humoral (CONICET-UBA), Universidad de Buenos Aires, Argentina.
Borel
I M
IM
Freire
S M
SM
Binaghi
R
R
Margni
R
R
D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000187:drug effects; D002478:Cells, Cultured; D003433:Crosses, Genetic; D005260:Female; D006825:Hybridomas / Q000737:chemistry; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000187:drug effects; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010920:Placenta / Q000737:chemistry / Q000276:immunology* / Q000502:physiology*
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G