Simple differentiation between core-fucosylated and nonfucosylated glycans by capillary electrophoresis.
Core-fucosylated glycans, derivatized with 2-aminoacridone, consistently migrate slower than the corresponding oligosaccharides which lack this fucose residue, using the micellar electrophoretic capillary chromatography conditions outlined in this study. alpha-Fucosidase digestion of glycans followed by CE analysis has allowed facile differentiation of these two classes of oligosaccharides and this methodology has been applied to obtain preliminary information on the carbohydrate content from two glycoproteins, a monoclonal IgG antibody and the soluble complement receptor type 1 (sCR1).
Okafo
G N
GN
SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, Hertfordshire, AL6 9AR, United Kingdom.
Burrow
L M
LM
Neville
W
W
Truneh
A
A
Smith
R A
RA
Reff
M
M
Camilleri
P
P
D000609:Aminoacridines; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D005643:Fucose / Q000032:analysis*; D007074:Immunoglobulin G / Q000737:chemistry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*; D011951:Receptors, Complement / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D005644:alpha-L-Fucosidase / Q000378:metabolism
D000609:Aminoacridines; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D011134:Polysaccharides; D011951:Receptors, Complement; D011994:Recombinant Proteins; C082885:soluble complement inhibitor 1; C072515:2-aminoacridone; D005643:Fucose; D005644:alpha-L-Fucosidase