Size-exclusion liquid chromatography and capillary electrophoresis of pollen allergens.
Allergens from the pollen of Phleum pratense, Dactylis glomerata. Arrhenatherum elatius, Secale cereale, Lolium perrene and Festuca sp. were analysed by size-exclusion chromatography (SEC) and capillary electrophoresis (CE). SEC was used for the determination of the molecular masses of main allergens. A CE method, using either 150 mmol/l phosphoric acid (pH 1.8) or a micellar system consisting of 50 mmol/l sodium dodecyl sulphate-20 mmol/l borate (pH 9.35), was developed as a rapid and efficient alternative to SEC, especially for process control of allergenic preparations. The results obtained by the two methods confirmed similarities in the structures of the studied pollen allergens.
Pacáková
V
V
Department of Analytical Chemistry, Charles University, Prague, Czech Republic.
Pechancová
J
J
Stulík
K
K
D000485:Allergens / Q000032:analysis* / Q000737:chemistry*; D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011058:Pollen / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000485:Allergens; D002021:Buffers