Maintenance of imprinting of the insulin-like growth factor II gene (IGF2) and the small nuclear ribonucleoprotein polypeptide N gene (SNRPN) in the human uterus and leiomyoma.
The insulin-like growth factor II gene (IGF2) is thought to be involved in the growth of uterine smooth muscle tumors. We studied the allele-specific expression of IGF2 in 20 patients with uterine leiomyomas by analyzing restriction fragment length polymorphisms (RFLP), because IGF2 is a maternally imprinted gene and only the paternal allele is exclusively expressed in human somatic tissue. We also studied the allelic expression of the small nuclear ribonucleoprotein polypeptide N gene (SNRPN), which is reportedly maternally imprinted in humans, and compared the imprinting status with that of IGF2. Nine patients (45%) were heterozygous at the ApaI site of IGF2, nine (45%) were heterozygous at the possible AccII polymorphic site of SNRPN, and three (15%) showed polymorphism in both genes. The genomic DNA of 15 patients showed heterozygosity in either or both of these genes, and the mRNA of these was expressed monoallelically in myometrial tissues and leiomyomas of these patients. These results demonstrated that IGF2 and SNRPN imprinting is completely maintained in human uteri and leiomyomas and that increased expression of IGF2 is not due to biallelic expression.
Hashimoto
K
K
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Azuma
C
C
Kamiura
S
S
Koyama
M
M
Nobunaga
T
T
Tokugawa
Y
Y
Kimura
T
T
Kubota
Y
Y
Sawai
K
K
Saji
F
F
D001324:Autoantigens / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D006579:Heterozygote; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007889:Leiomyoma / Q000235:genetics*; D008969:Molecular Sequence Data; D009215:Myometrium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear; D014594:Uterine Neoplasms / Q000235:genetics*; D014599:Uterus / Q000378:metabolism*; D055517:snRNP Core Proteins
D001324:Autoantigens; D012333:RNA, Messenger; D017411:Ribonucleoproteins, Small Nuclear; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D007335:Insulin-Like Growth Factor II; D004247:DNA