title: Seizures induced by alpha-guanidinoglutaric acid, a nitric oxide synthase inhibitor, are controlled by L-arginine.
abstract: We evaluated the effect of manipulation of nitric oxide (NO) synthesis on epileptiform discharges recorded from immobilized rats during intracerebroventricular injection of alpha-guanidinoglutaric acid (GGA), an endogenous convulsant and a NO synthase (NOS) inhibitor, alone or in combined with a NOS substrate, l-arginine (ARG). GGA alone, or combined with 50 mM ARG, resulted in prolonged electrographical seizures while co-injection of either 100 or 200 mM of ARG with GGA caused significantly protection. These data show that ARG inhibited epileptiform discharges in a dose-dependent fashion, suggesting that the discharges initiated by inhibition of NOS with the intrinsic convulsant GGA are abated by increasing the concentration of the NOS substrate ARG.
authors.affiliation: Department of Neuroscience, Okayama University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Habu
authors.forename: I
authors.initials: I
authors.lastname: Yokoi
authors.forename: H
authors.initials: H
authors.lastname: Kabuto
authors.forename: L J
authors.initials: LJ
authors.lastname: Willmore
authors.forename: A
authors.initials: A
authors.lastname: Mori
mesh_terms: D000818:Animals; D001120:Arginine; D004569:Electroencephalography; D005977:Glutarates; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced*
chemical_list: D005977:Glutarates; C028262:alpha-guanidinoglutaric acid; D001120:Arginine; D019001:Nitric Oxide Synthase