Cortical cell plasma membrane alterations after in vitro alcohol exposure: prevention by GM1 ganglioside.
Using choleratoxin/antitoxin immunohistochemistry, this study examined the effects of in vitro alcohol exposure on the morphology of cell plasma membranes in mixed fetal rat cortical cultures, and assessed the neuroprotective effects of exogenous monosialoganglioside (GM1). Gangliosides are involved in critical biological functions, including maintenance of membrane integrity. Plasma membranes are directly affected by alcohol exposure through multiple mechanisms. Results indicate that exposure to alcohol altered plasma membrane morphology as assessed by staining for the surface distribution of membrane GM1. Pretreatment with endogenous GM1 ameliorated the alcohol-induced alterations.
Laev
H
H
Division of Neuroscience, New York State Psychiatric Institute, NY, USA.
Hungund
B L
BL
Karpiak
S E
SE
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000633:toxicity*; D002540:Cerebral Cortex / Q000187:drug effects / Q000648:ultrastructure*; D000431:Ethanol / Q000633:toxicity*; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D007150:Immunohistochemistry; D000922:Immunotoxins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D002492:Central Nervous System Depressants; D000922:Immunotoxins; D005677:G(M1) Ganglioside; D000431:Ethanol