title: Cloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94.
abstract: A gene which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I of homology) and total resistance to the small isometric-headed bacteriophage phi 59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.
authors.affiliation: Systems Bio-Industries, Boulogne Billancourt, France.
authors.forename: F
authors.initials: F
authors.lastname: Prévots
authors.forename: M
authors.initials: M
authors.lastname: Daloyau
authors.forename: O
authors.initials: O
authors.lastname: Bonin
authors.forename: X
authors.initials: X
authors.lastname: Dumont
authors.forename: S
authors.initials: S
authors.lastname: Tolou
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001435:Bacteriophages / Q000472:pathogenicity*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D007113:Immunity, Innate / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics* / Q000821:virology*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins