title: A role for ADP-ribosylation factor 1, but not COP I, in secretory vesicle biogenesis from the trans-Golgi network.
abstract: A synthetic N-myristoylated peptide corresponding to the amino-terminal domain of ADP-ribosylation factor 1 (ARF1) markedly increases, in a cell-free system using post-nuclear supernatant from PC12 cells, the biogenesis of constitutive secretory vesicles and immature secretory granules from the trans-Golgi network (TGN). The related N-myristoylated ARF4 peptide only weakly stimulates, and the non-myristoylated ARF1 and ARF4 peptides inhibit, the biogenesis of these secretory vesicles. In a modified cell-free system using TGN membranes, coatomer-depleted cytosol supports the biogenesis of TGN-derived secretory vesicles to the same extent as control cytosol. These results suggest a role for ARF1, but not the COP I coat, in secretory vesicle biogenesis from the TGN, possibly via the activation of phospholipase D.
authors.affiliation: Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: F A
authors.initials: FA
authors.lastname: Barr
authors.forename: W B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002474:Cell-Free System; D003594:Cytoplasmic Granules / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D016716:PC12 Cells; D010940:Plant Proteins / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D044767:Ubiquitin-Protein Ligases
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D009227:Myristic Acids; D010940:Plant Proteins; D013431:Sulfates; D013462:Sulfur Radioisotopes; D019814:Myristic Acid; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000255:Adenosine Triphosphate; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors