Protecting effect of a green tea percolate and its main constituents against gamma ray-induced scission of DNA.
Gamma ray-induced scission of puC18 plasmid DNA prepared from E. coli was examined in the presence of a green tea percolate and its main constituents, L-ascorbic acid (used as the sodium salt) and (-)-epigallocatechin gallate. Each of these showed a protecting effect against DNA scission. The relationship between the protecting effect against DNA scission and the scavenging effect of the hydroxyl radical was examined, and is discussed from the viewpoint of interaction with DNA.
Yoshioka
H
H
Radiochemistry Research Laboratory, Shizuoka University, Japan.
Akai
G
G
Yoshinaga
K
K
Hasegawa
K
K
Yoshioka
H
H
D016587:Antimutagenic Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays / Q000009:adverse effects*; D017665:Hydroxyl Radical / Q000009:adverse effects; D010936:Plant Extracts / Q000494:pharmacology; D010957:Plasmids; D013237:Stereoisomerism; D013662:Tea / Q000378:metabolism
D016587:Antimutagenic Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013662:Tea; D017665:Hydroxyl Radical; D002392:Catechin; C045651:epigallocatechin gallate; D001205:Ascorbic Acid