Immune response to 17 beta-estradiol in polyelectrolyte complex: antigen specificity and affinity of hybridoma clones.
Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R2, R16)], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water. Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R2, R16) solution at pH 7. A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place. The immunogenic properties of PVP (R2, R16)-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed. 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse. However, a comparative study of immunogenic activity of polycomplexes versus BSA.E+incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production. In contrast to IFA+BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies. Such a carrier-directed response may be determined by increase in immunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes. Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities. Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.
Başalp
A
A
Institute for Genetic Engineering and Biotechnology, Tübitak Marmara Research Center, Kocaeli, Turkey.
Bermek
E
E
Cirakoğlu
B
B
Coka
V
V
Mustafaev
M I
MI
Saraç
A S
AS
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004958:Estradiol / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000276:immunology; D011145:Polyvinyls; D012710:Serum Albumin, Bovine
D000939:Epitopes; D011145:Polyvinyls; C027968:poly-4-vinyl-N-ethylpyridine; D012710:Serum Albumin, Bovine; D004958:Estradiol