Effect of temperature on the high-performance liquid chromatographic separation of the anti-HIV agents, didanosine and stavudine.
This paper examines the effect of temperature on the chromatographic separation characteristics of anti-HIV agents, didanosine and stavudine. As a result of lowering the column temperature, an improved resolution between didanosine and stavudine peaks is observed. Thus, lower temperatures may permit the simultaneous monitoring of didanosine and stavudine levels.
Stancato
F A
FA
Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543, USA.
Srinivas
N R
NR
Knupp
C A
CA
D000998:Antiviral Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D016049:Didanosine / Q000302:isolation & purification*; D006678:HIV / Q000187:drug effects*; D018119:Stavudine / Q000302:isolation & purification*; D013696:Temperature
D000998:Antiviral Agents; D018119:Stavudine; D016049:Didanosine