Calcium/calmodulin-dependent protein kinase II and arrestin phosphorylation in Limulus eyes.
In rhabdomeral photoreceptors, light stimulates the phosphorylation of arrestin, a protein critical for quenching the photoresponse, by activating a calcium/calmodulin-dependent protein kinase (CaM PK). Here we present biochemical evidence that a CaM PK that phosphorylates arrestin in Limulus eyes is structurally similar to mammalian CaM PK II. In addition, cDNAs encoding proteins homologous to mammalian and Drosophila CaM PK II in the catalytic and regulatory domains were cloned and sequenced from a Limulus lateral eye cDNA library. The Limulus sequences are unique, however, in that they lack most of the association domain. The proteins encoded by these sequences may phosphorylate arrestin.
Calman
B G
BG
Whitney Laboratory, University of Florida, St. Augustine 32086, USA.
Andrews
A W
AW
Rissler
H M
HM
Edwards
S C
SC
Battelle
B A
BA
D000595:Amino Acid Sequence; D000818:Animals; D019393:Arrestin / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005123:Eye; D006737:Horseshoe Crabs / Q000378:metabolism*; D008027:Light; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D010766:Phosphorylation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000166:cytology; D013379:Substrate Specificity
D019393:Arrestin; D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium