Role for CD30 in HIV expression.
CD30 is a member of the tumor necrosis factor (TNF)-receptor superfamily, whose ligand (CD30L) has been identified on B cells, activated macrophages and a subset of activated T cells. We show here that infection in vitro with human immunodeficiency virus (HIV) of CD4+ T-cell clones generated from HIV-seronegative individuals can enhance the expression of CD30, which often preceeds and is associated with the death of clonal T cells. Furthermore, cross-linking CD30 with an agonistic CD30-specific monoclonal antibody potentiated HIV replication induced by an insolubilized anti-CD3 antibody in T-cell lines generated from HIV-infected individuals. More importantly, paraformaldehyde-fixed CD8+ T-cell clones expressing CD30L enhanced HIV replication in anti-CD3-stimulated allogeneic or autologous HIV-infected CD4+ T-cell lines and such a potentiating effect was inhibited by an anti-CD30L antibody. The anti-CD30L antibody also exerted a suppressive effect on the spontaneous HIV replication occurring in lymph node cells, freshly derived from an HIV-seropositive patient showing CD30 expression in B cells and in a proportion of CD8+ T lymphocytes. Thus, CD30 triggering may play an important role in both HIV replication and the death of HIV-infected CD4+ T cells.
Romagnani
S
S
Institute of Internal Medicine and Immunoallergology, University of Florence, Italy.
Annunziato
F
F
Manetti
R
R
Almerigogna
F
F
Biagiotti
R
R
Giudizi
M G
MG
Ravina
A
A
Giannò
V
V
Tomasévic
L
L
Maggi
E
E
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D053285:CD30 Ligand; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000821:virology; D016923:Cell Death / Q000276:immunology; D002460:Cell Line; D006678:HIV / Q000187:drug effects*; D015658:HIV Infections / Q000276:immunology; D006801:Humans; D017730:Ki-1 Antigen / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011485:Protein Binding / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D053285:CD30 Ligand; D017730:Ki-1 Antigen; D008024:Ligands; D008562:Membrane Glycoproteins; C505774:TNFSF8 protein, human