Regulation of pancreatitis-associated protein (HIP/PAP) mRNA levels in mouse pancreas and small intestine.
1. In the present study we have examined the expression of pancreatitis-associated protein mRNA in mouse pancreas and small intestine and determined the effect of a number of factors on the steady-state level of the RNA. 2. The normally low level of pancreatitis-associated protein mRNA in pancreas increased severalfold after 6 h of hypoxia, reaching peak levels (approximately 10-fold greater than normal) after 24 h hypoxia. After 3 days' hypoxia pancreatitis-associated protein mRNA levels were again undetectable. 3. In the pancreas the level of pancreatitis-associated protein mRNA was also increased by alcohol and iron overload, but not by paracetamol. 4. In the small intestine expression of pancreatitis-associated protein mRNA was higher in normal ileum than in duodenum. In the ileum pancreatitis-associated protein mRNA levels were increased 7 to 15-fold after 6 h hypoxia, reaching peak levels by 24 h. Levels declined after 3 days' hypoxia, but remained higher than normal. 5. In the ileum long-term (4 weeks) dietary iron deficiency reduced pancreatitis-associated protein mRNA levels compared with control fed mice, whereas parenteral iron overload increased pancreatitis-associated protein mRNA levels. 6. The data presented suggest regulation of pancreatitis-associated protein gene expression by both oxygen tension and iron status.
McKie
A T
AT
Department of Molecular Medicine, King's College School of Medicine and Dentistry, London, U.K.
Simpson
R J
RJ
Ghosh
S
S
Peters
T J
TJ
Farzaneh
F
F
D000082:Acetaminophen / Q000494:pharmacology; D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002460:Cell Line; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005786:Gene Expression Regulation; D000860:Hypoxia / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D037181:Lectins, C-Type; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010179:Pancreas / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors
D000209:Acute-Phase Proteins; D018712:Analgesics, Non-Narcotic; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; D012333:RNA, Messenger; C490253:Reg3b protein, mouse; D000082:Acetaminophen; D000431:Ethanol; D007501:Iron