The effect of the lipid peroxidation product 4-hydroxynonenal and of its metabolite 4-hydroxynonenoic acid on respiration of rat kidney cortex mitochondria.
In rat kidney cortex mitochondria, 4-hydroxynonenal inhibits state 3 respiration as well as uncoupled respiration at micromolar concentrations. The inhibition is more distinct for NAD-linked than for FAD-linked respiration. 4-Hydroxynonenal increases the state 4 respiration. It is assumed that 4-hydroxynonenal behaves like a decoupling agent. 4-Hydroxynonenal augments the inhibitory effect of 2,4-dinitrophenol observed at superoptimal concentrations. 4-Hydroxynonenal is metabolised by renal mitochondria, and 4-hydroxynonenoic acid is one of the metabolites generated. This metabolite is without effect on respiration at concentrations up to 50 microM. Therefore, the effect of 4-hydroxynonenal on respiration is not mediated by this fatty acid derivative formed during respiratory measurements.
Ullrich
O
O
Clinics of Physical Therapy, University Hospital Charité, Medical Faculty, Humboldt University, Berlin, Germany.
Henke
W
W
Grune
T
T
Siems
W G
WG
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002474:Cell-Free System; D006880:Hydroxy Acids / Q000378:metabolism / Q000494:pharmacology*; D007672:Kidney Cortex / Q000378:metabolism*; D008054:Lipid Peroxides / Q000737:chemistry*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
C091766:4-hydroxy-2-nonenoic acid; D000447:Aldehydes; D006880:Hydroxy Acids; D008054:Lipid Peroxides; C027576:4-hydroxy-2-nonenal