title: Sample preparation for taxol and cephalomannine determination in various organs of Taxus sp.
abstract: Solid-phase extraction and preparative thin-layer chromatography were applied as sample preparation techniques for the purification of crude extracts from twigs and needles of various Taxus species as well as for the isolation of taxol and cephalomannine for further reversed phase high performance liquid chromatography analysis. Significant differences in the contents of taxanes examined were found. The preparative chromatographic methods used were compared and evaluated as routine and reproducible procedures for the rapid isolation and determination of taxol and cephalomannine in plant extracts.
authors.affiliation: Department of Pharmacognosy, Medical Academy, Lublin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Glowniak
authors.forename: G
authors.initials: G
authors.lastname: Zgórka
authors.forename: A
authors.initials: A
authors.lastname: Józefczyk
authors.forename: M
authors.initials: M
authors.lastname: Furmanowa
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis; D043823:Taxoids; D014197:Trees
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D043823:Taxoids; C056348:cephalomannine; D017239:Paclitaxel