title: Physical maps of the chromosomes of Haemophilus influenzae Sb.
abstract: The physical maps the Haemophilus influenzae Sb genomes were constructed by the comparison of restriction fragment sizes of complete single and double digests, as well as by the analysis of partial DNA digests. Sites for four restriction endonucleases NotI, RsrII, SmaI and SrfI were located on the bacterial chromosomes, which are circular, and 2028 and 2045 kb in circumferences. The order of fragments was deduced from fragment patterns of the combinations of double digestion and by two-dimensional Field-Inversion Gel Electrophoresis (FIGE).
authors.affiliation: Institute of Microbiology, Warsaw University, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Kauc
authors.forename: A
authors.initials: A
authors.lastname: Michałowska
authors.forename: M
authors.initials: M
authors.lastname: Gnas-Wiernicka
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial