Primary cultivation of human synovial cells from nonrheumatic synovial tissue and fluid.
Cultures were established from adherent cells of synovial fluid and from collagenase-dispersed specimens of synovial tissue. In cultures derived from synovial tissue, prolyl hydroxylase-positive cells of fibroblast-like morphology were identified as the predominant cell type. In cultures from synovial fluid the majority of adherent cells was macrophage-like in appearance and strongly positive for CD 68. Cells with a stellate morphology could rarely be observed in cultures from synovial tissue. Their relations to other forms will be discussed. Several simple methods for cultivating adherent synovial cells are presented.
Rödel
J
J
Institut für Medizinische Mikrobiologie, Friedrich-Schiller-Universität Jena, Germany.
Straube
E
E
Lungershausen
W
W
Roth
A
A
Groh
A
A
D018929:Cell Culture Techniques; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007596:Joints / Q000166:cytology*; D013582:Synovial Fluid / Q000166:cytology*; D013583:Synovial Membrane / Q000166:cytology*