A gene highly homologous to ACP1 encoding cysteine proteinase 3 in Entamoeba histolytica is present and expressed in E. dispar.
We have cloned the complete gene encoding cysteine proteinase 3 in E. histolytica as well as a cDNA encoding a highly homologous protein in E. dispar. In addition, we have demonstrated the presence and expression of the CP3 gene in various E. histolytica and E. dispar isolates. Since the expression of the gene is rather similar within both Entamoeba species, we assume that it does not constitute the proposed virulence factor of E. histolytica.
Bruchhaus
I
I
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Tannich
E
E
D000818:Animals; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D004747:Entamoeba / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology*; D015870:Gene Expression; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases