title: N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor antagonists differentially suppress dorsal horn neuron responses to mechanical stimuli in rats with peripheral nerve injury.
abstract: The effects of iontophoretically ejected glutamate receptor antagonists on mechanically evoked responsiveness were examined on wide dynamic range (WDR) dorsal horn neurons in anesthetized rats that received a unilateral ligation of the L5 and L6 spinal nerves 10-15 days previously. Both brush- and pinch-evoked responses of dorsal horn neurons on the nerve-injured side were enhanced. N-Methyl-D-aspartate (NMDA) receptor antagonist, 2-amino-5-phosphonopentanoic acid (AP5), preferentially suppressed the enhanced pinch-evoked response, whereas (RS)-a-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) receptor selective antagonist, 6-nitro-7-sulphamoylbenzo(f)quinoxaline-2,3-dione (NBQX), preferentially attenuated the enhanced brush-evoked response. The results indicate that the enhanced responses to noxious and non-noxious peripheral inputs induced in WDR dorsal horn neurons following the nerve injury are mediated by activation of NMDA and AMPA receptors, respectively.
authors.affiliation: Department of Physiology, Yonsei University College of Medicine, Seoul, South Korea. jwleem@msmail.yonsei.ac.kr
authors.forename: J W
authors.initials: JW
authors.lastname: Leem
authors.forename: E J
authors.initials: EJ
authors.lastname: Choi
authors.forename: E S
authors.initials: ES
authors.lastname: Park
authors.forename: K S
authors.initials: KS
authors.lastname: Paik
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D004594:Electrophysiology; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D007478:Iontophoresis; D008297:Male; D016202:N-Methylaspartate / Q000008:administration & dosage / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D059348:Peripheral Nerve Injuries; D010812:Physical Stimulation; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D016202:N-Methylaspartate; D015763:2-Amino-5-phosphonovalerate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid