Development and validation of a high-performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine.
Street cocaine varies in purity and is often adulterated with various compounds. Some of these additives, such as lidocaine, may increase the toxicity of cocaine. A new precise, accurate and sensitive reversed-phase high-performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine in plasma samples has been developed and validated. This assay employed a phosphate-buffered aqueous mobile phase (pH 6.0) with an organic component consisting of acetonitrile and methanol and a C-18 column as the stationary phase. Minimum detection limits were 1 ng ml-1 for cocaine, 2.5 ng ml-1 for ethylcocaine and 5 ng ml-1 for benzoylecgonine, norcocaine, norethylcocaine and lidocaine. Linearity was demonstrated over a broad range of concentrations in plasma, with good sensitivity for cocaine and cocaine derivatives.
Barat
S A
SA
Department of Pharmacology and Toxicology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103-2714, USA.
Kardos
S A
SA
Abdel-Rahman
M S
MS
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D008012:Lidocaine / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D008012:Lidocaine; D003042:Cocaine