Sculpted immunogens; B-cell epitope optimization using constrained secondary structure libraries.
A library of conformationally constrained reverse turn mimetics of the HIV V3 loop was screened against the mAb 50.1. Addition of a Th-cell epitope to the reverse turn template provided an immunogen which, when immunized (either in alum or IFA), generated a high titer, highly specific response to HIV gp 120.
Urban
J
J
Molecumetics, Ltd, Bellevue, Washington 98005, USA.
Qabar
M
M
Sia
C
C
Klein
M
M
Kahn
M
M
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology*; D006168:Guinea Pigs; D015488:HIV Antigens / Q000737:chemistry / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D017433:Protein Structure, Secondary; D014614:Vaccines, Synthetic / Q000737:chemistry* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D018985:Epitopes, B-Lymphocyte; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D014614:Vaccines, Synthetic