Candida albicans stress mannoprotein, SMP200, enhances tumour necrosis factor secretion in the murine macrophage cell line ANA-1.
Tumour necrosis factor (TNF) secretory activity has been studied in the murine macrophage cell line ANA-1 following in vitro exposure to Candida albicans 200 kDa stress mannoprotein (SMP200). Treatment of ANA-1 murine macrophages with 200 kDa stress mannoprotein results in increased TNF secretion. The phenomenon is (i) dose- and time-dependent, (ii) abrogated by 200 kDa stress mannoprotein preincubation with a specific monoclonal antibody, and (iii) dependent on intact murine macrophage Ca2+/calmodulin-dependent protein kinase function.
Pitzurra
L
L
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Polonelli
L
L
Cantelli
C
C
Gerloni
M
M
Ponton
J
J
Bikandi
J
J
Blasi
E
E
D000818:Animals; D002176:Candida albicans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D008562:Membrane Glycoproteins; D047428:Protein Kinase Inhibitors; D014409:Tumor Necrosis Factor-alpha; C025363:mannoproteins; D011494:Protein Kinases