title: Active oxygen intermediates and chlorophyllin bleaching.
abstract: Bleaching of chlorophyllin, a water soluble copper containing porphyrin molecule, was investigated with regard to the potential role of active oxygen intermediate involvement. It was found that the bleaching was highly aerobic and also biphasic in nature. The aerobic photobleaching and the dark bleaching were effectively prevented by the addition of reductants such as ascorbate and cysteine. In addition, the reductant and peroxyl radical scavenger, Trolox, was highly effective in preventing bleaching. Catalase was moderately effective in preventing photobleaching whereas peroxidase and superoxide dismutase hastened the photobleaching process. It is concluded that the bleaching of chlorophyllin is a peroxidative process which does not involve singlet oxygen, superoxide, nor the .OH radical.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi 39762, USA.
authors.forename: A
authors.initials: A
authors.lastname: Penttilä
authors.forename: C R
authors.initials: CR
authors.lastname: Boyle
authors.forename: M L
authors.initials: ML
authors.lastname: Salin
mesh_terms: D002735:Chlorophyllides / Q000737:chemistry*; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species
chemical_list: D002735:Chlorophyllides; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; C007020:chlorophyllin