Presentation of peptide antigens as albumin conjugates for use in detection of serum antibodies by enzyme-linked immunosorbent assay.
The use of linear peptides as antigens for detection of serum antibodies has been studied using a sequence of the Borrelia burgdorferi protein, flagellin, and Lyme disease sera as a model. It was found that a novel presentation of the peptide as a hapten on the carrier protein, bovine serum albumin, in the enzyme-linked immunosorbent assay format can be successfully applied to distinguish between Lyme disease and control sera.
Yu
Z
Z
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, USA.
Carter
J M
JM
Huang
S Y
SY
Lackland
H
H
Sigal
L H
LH
Stein
S
S
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000097:blood*; D000907:Antibodies, Bacterial / Q000097:blood; D015748:Borrelia burgdorferi Group / Q000276:immunology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005408:Flagellin / Q000097:blood / Q000276:immunology; D006801:Humans; D008193:Lyme Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014614:Vaccines, Synthetic / Q000737:chemistry*
D000596:Amino Acids; D000906:Antibodies; D000907:Antibodies, Bacterial; D000939:Epitopes; D014614:Vaccines, Synthetic; D005408:Flagellin; D012710:Serum Albumin, Bovine; D003545:Cysteine