title: Human meniscal proteoglycan metabolism in long-term tissue culture.
abstract: For the purpose of human meniscal allografting, menisci have been maintained viable in in vitro culture. The influence of long-term tissue culture on the extracellular matrix metabolism of the meniscus has been studied. Fetal calf serum (FCS) was used as a supplement for the growth factors necessary to maintain optimal meniscal cell metabolism. A series of semilunar cartilage samples was cultured under serum-free conditions since foreign proteins could be responsible for immunological problems after eventual allografting. The proteoglycan metabolism in human menisci cultured in FCS-supplemented and in serum-free culture media was compared. To rule out any influence of topographical variations in glycosaminoglycan (GAG) content on proteoglycan (PG) metabolism, GAG concentrations within the tissue were determined, and sulphate (35S) incorporation was studied in tissue samples with a comparable biochemical composition. Sulphate incorporation was preserved when 20% FCS was added to the nutrient medium. The meniscal tissue fibroblasts continued to produce 35S-PG during 4 weeks of culture. The PG molecules were shown to consist of PG-aggregates, monomers and a low molecular-weight PG population. Newly synthesized GAG consisted of approximately 55% chondroitin 4- and 6-sulphate and 33% dermatan sulphate. In the presence of serum, 35S incorporation in PG and in the PG-aggregate fraction significantly increased during the first 2 weeks and then decreased during the following 2 weeks of in vitro culture. Newly synthesized PG-aggregates were almost entirely accumulated in the tissue during these weeks. In the 3rd week the values for this parameter decreased slightly. 35S-PG synthesis dramatically declined after 4 weeks of in vitro culture. Catabolism probably resulted in increased proportions of 35S-PG in the incubation media. In the absence of serum, 35S-PG production also increased in the 2nd week of culture. However, 35S activity was almost exclusively found in small PG, and this material apparently diffused to the incubation media. Consequently, catabolism is higher, and the immobilization of 35S-PG is poor when FCS is not added to the culture media. Our findings suggest that menisci are maintained in viable condition and may serve for allografting at least during 2 weeks of tissue culture.
authors.affiliation: Department of Rheumatology, Ghent University Hospital, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Verbruggen
authors.forename: R
authors.initials: R
authors.lastname: Verdonk
authors.forename: E M
authors.initials: EM
authors.lastname: Veys
authors.forename: P
authors.initials: P
authors.lastname: Van Daele
authors.forename: P
authors.initials: P
authors.lastname: De Smet
authors.forename: K
authors.initials: K
authors.lastname: Van den Abbeele
authors.forename: B
authors.initials: B
authors.lastname: Claus
authors.forename: D
authors.initials: D
authors.lastname: Baeten
mesh_terms: D000328:Adult; D000368:Aged; D002102:Cadaver; D002850:Chromatography, Gel; D046508:Culture Techniques; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans