alpha-subunit of CaM-KII increases glycine currents in acutely isolated rat spinal neurons.
1. Here we report that in acutely isolated rat spinal dorsal horn neurons, the glycine receptor can be regulated by exogenous calcium/ calmodulin-dependent protein kinase II (CaM-KII). Intracellularly applied, the alpha-subunit of CaM-KII enhanced glycine receptor-activated current recorded with the use of the whole cell patch-clamp technique. This result suggests that the function of glycine receptor is modulated by CaM-KII, but the cellular mechanism underlying the enhancement of glycine receptor-activated current is still unknown.
Wang
R A
RA
Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames 50011, USA.
Randić
M
M
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000494:pharmacology*; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018009:Receptors, Glycine / Q000187:drug effects*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D018118:Chloride Channels; D018009:Receptors, Glycine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005998:Glycine