Ultraviolet-A induces activation of AP-1 in cultured human keratinocytes.
UV-A irradiation induces a time-dependent activation of AP-1 in NCTC 2544 human keratinocytes. 4 h after irradiation, a 2-3-fold increase in AP-1 activity is observed in human keratinocytes and fibroblasts. Activation is still detectable 24 h later. The UV-A induced AP-1 binding complex is shown to contain c-Fos and c-Jun proteins. Lipophilic vitamin E impedes UV-A induced lipid peroxidation but does not prevent AP-1 activation which is inhibited by N-acetylcysteine, a hydrophilic antioxidant. This finding suggests that UV-A-dependent AP-1 activation is sensitive to the cellular redox state but is not related to membrane lipid peroxidation.
Djavaheri-Mergny
M
M
Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, Paris, France.
Mergny
J L
JL
Bertrand
F
F
Santus
R
R
Mazière
C
C
Dubertret
L
L
Mazière
J C
JC
D000111:Acetylcysteine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D007700:Kinetics; D010084:Oxidation-Reduction; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D017392:Thiobarbituric Acid Reactive Substances; D018808:Transcription Factor AP-1; D014810:Vitamin E; D000111:Acetylcysteine