Improved isolation of genomic DNA from mycobacteria in agarose plugs by rapid lysis with a combination of N-acetylglucosaminidase and lysozyme.
Choe
Y K
YK
Dong-Guk University Kyungpook, Korean Institute of Tuberculosis, Seoul, South Korea.
Huh
Y J
YJ
Park
J H
JH
Kim
J R
JR
Park
J S
JS
Song
J C
JC
Ko
J H
JH
Lee
Y C
YC
Nashiru
O
O
Kim
J K
JK
Kim
S J
SJ
Bai
G H
GH
Chung
T H
TH
Chung
T W
TW
Choe
I S
IS
Kim
C H
CH
D000118:Acetylglucosaminidase; D001433:Bacteriolysis; D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D006358:Hot Temperature; D009113:Muramidase; D009161:Mycobacterium / Q000235:genetics*; D009162:Mycobacterium avium / Q000235:genetics; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics; D009163:Mycobacterium bovis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D012685:Sepharose; D013997:Time Factors
D004269:DNA, Bacterial; D012685:Sepharose; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific; D009113:Muramidase; D000118:Acetylglucosaminidase