Allele assignment for HLA-A, -B, and -C genes to the Tenth International Histocompatibility Workshop cell lines.
Development of DNA typing for Class I HLA alleles has lagged behind that of class II for a variety of technical reasons. Following the recognition of locus specific sequences in the first and the third intron, and acquiring the ability to amplify genomic DNA by intron-based PCR primer, we have devised DNA typing of class I alleles by SSOP and direct sequencing. In this study using these techniques we provide the allelic typing of HLA-A, -B, and -C genes for the B-lymphoblastoid reference cell lines from the Tenth International Histocompatibility Workshop. We also describe some common associations of the C alleles with HLA-A and HLA-B alleles.
Prasad
V K
VK
Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, USA. v-prasad@skimskec.org
Yang
S Y
SY
D000483:Alleles; D002461:Cell Line, Transformed; D004493:Education; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006648:Histocompatibility; D006801:Humans
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens