Prostanoid modulation of glucose transport in isolated diabetic rat uterus.
3-O-14C-methyl-D-Glucose (3-O-MG) transport and 14C-saccharose incorporation were measured in isolated uterine strips from ovariectomized-estrogenized diabetic rats. Glucose transport was decreased in uterine strips from diabetic rats compared with control animals. PGE1 and PGE2 (10(-7) M) stimulated 3-O-MG transport, PGF2 alpha failed to modify this parameter at the same concentration, while insulin (0.5 U/ml) evoked an improvement 30% greater than PGs. In spite of the negative influence exerted by TXA2 over glucose metabolism in the isolated rat uterus, U46619, 10(-5) M (a TXA2 analogue), and OKY064, 10(-7) M (an inhibitor of TXA2 synthesis), failed to modify basal or insulin-treated hexose transport. Neither additive or synergistic interactions between PGE1 or PGE2 (10(-7) M) and insulin at 0.5 U/ml and 0.25 U/ml were detected. We conclude that the stimulatory action of PGE1 and PGE2 on glucose metabolism that has been previously described by us, involves enhancement of glucose transport.
González
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ET
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Cientificas y Ténicas (CONICET), Buenos Aires, Argentina.
Jawerbaum
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A
Novaro
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Gimeno
M A
MA
D019325:3-O-Methylglucose / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013395:Sucrose / Q000378:metabolism; D013929:Thromboxane B2 / Q000037:antagonists & inhibitors; D015854:Up-Regulation; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D007328:Insulin; D019325:3-O-Methylglucose; D013929:Thromboxane B2; D013395:Sucrose; D013311:Streptozocin; D015237:Dinoprost; D000527:Alprostadil; D015232:Dinoprostone