Single radial haemolysis for the detection of goat pox virus antigen and antibody.
The single radial haemolysis (SRH) test was standardised for the detection of goat pox antigen and antibody. In this test soluble antigens, and serum prepared against soluble antigens were used for coupling sheep erythrocytes to detect goat pox antibody and antigen, respectively. This test was compared with the agar gel precipitation test (AGPT) and counter immunoelectrophoresis (CIE) test; the 2 most commonly used tests for goat pox diagnosis. SRH was found to be as sensitive as AGPT and CIE in detecting antigen and antibody (chi 2 = 2.9, P > 0.05).
Tiwari
A K
AK
Indian Veterinary Research Institute, Mukteswar Kumaon, India.
Negi
B S
BS
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis*; D018151:Capripoxvirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D015511:Goat Diseases / Q000276:immunology; D006041:Goats; D006462:Hemolytic Plaque Technique / Q000592:standards / Q000706:statistics & numerical data / Q000662:veterinary*; D011213:Poxviridae Infections / Q000276:immunology / Q000662:veterinary; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012995:Solubility
D000914:Antibodies, Viral; D000956:Antigens, Viral