title: Histochemistry of NADPH-diaphorase--a marker for neuronal nitric oxide synthase--in the carp retina.
abstract: The nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemical technique was used as a marker to assess the distribution of nitric oxide synthase activity in the carp retina. NADPH-diaphorase activity was found to be present in photoreceptors (rods and cones), horizontal cells, amacrine cells, bipolar cells, Müller cells and ganglion cells. Staining was most prominent in the photoreceptor ellipsoids but was not confined to any particular subtype. The density of the staining within the inner plexiform layer (IPL) was determined by image analysis. There was a broad peak of activity in each sublamina of the IPL, but sublamina b appeared to be relatively more heavily stained. The results taken together suggest that the nitric oxide signalling system could have a broader involvement in retinal function than previously thought. Furthermore, nitric oxide may have a novel mode of action in the retina whereby it could be effective on cells (photoreceptors) that also synthesize it.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
authors.forename: M B
authors.initials: MB
authors.lastname: Djamgoz
authors.forename: R
authors.initials: R
authors.lastname: Aguilo
authors.forename: E H
authors.initials: EH
authors.lastname: Greenstreet
authors.forename: R
authors.initials: R
authors.lastname: Reynolds
authors.forename: G P
authors.initials: GP
authors.lastname: Wilkin
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D006651:Histocytochemistry; D007091:Image Processing, Computer-Assisted; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000201:enzymology; D012160:Retina / Q000166:cytology / Q000201:enzymology*
chemical_list: D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase