BIRMA-K3, a new monoclonal antibody for CD34 immunophenotyping and stem and progenitor cell assay.
A murine monoclonal antibody with specificity for the CD34 antigen has been produced and designated BIRMA-K3. The antibody characterized as IgG1(kappa) has been shown to react with KG-1a cells following treatment of the cells with glycoprotease enzyme, indicating reactivity with the class III epitope of CD34. It was possible to show that class I and class II anti-CD34 antibodies were not able to inhibit binding of BIRMA-K3. Investigation of FITC-labeled as well as PE-labeled BIRMA-K3 resulted in a clear cut-off staining of acute leukemias and CD34+ cell counts in patients submitted to high-dose chemotherapy and stem cell transplantation. The results obtained correlate strongly with those from HPCA-2, the Becton-Dickinson class III antibody.
Höffkes
H G
HG
Department of Medicine, Otto-von-Guericke University of Magdeburg, Germany.
Lowe
J A
JA
Pedersen
R O
RO
Schmidkte
G
G
McDonald
D F
DF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D018952:Antigens, CD34 / Q000276:immunology*; D002460:Cell Line / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000939:Epitopes