Influence of uremic middle molecules on in vitro stimulated lymphocytes and interleukin-2 production.
The influence of uremic middle molecules on in vitro lymphocyte blast transformation and interleukin-2 production was studied in 12 patients on long-term hemodialysis and 12 healthy control subjects with normal kidney function. Middle molecules inhibit phytohemoagglutinin induced lymphocyte proliferation in a concentration dependent fashion, achieving more than 50% inhibition of tritiated thymidine incorporation after 72 hrs in culture. In addition, there was less interleukin-2 production in the long-term hemodialysis patients studied compared with healthy control subjects.
Severini
G
G
Laboratorio di Biochimica Clinica, Istituto Superiore di Sanità, Roma, Italia.
Diana
L
L
Di Giovannandrea
R
R
Sagliaschi
G
G
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects; D014118:Toxins, Biological / Q000097:blood / Q000633:toxicity*; D014511:Uremia / Q000097:blood / Q000276:immunology*
D007376:Interleukin-2; D014118:Toxins, Biological; C026865:uremia middle molecule toxins