title: High-performance liquid chromatography method with light-scattering detection for measurements of lipid class composition: analysis of brains from alcoholics.
abstract: A high-performance liquid chromatographic method with evaporative light-scattering detection was developed for the analysis of intact lipid classes in nervous tissue. The method had the ability to resolve plasmalogen-phosphatidyl-ethanolamine and diacyl-phosphatidylethanolamine along with other major phospholipid classes in a single run. This technique was employed for the investigation of the effects of chronic alcohol consumption on the membrane lipid class composition of human brains (alcoholics, n = 13; controls, n = 11). Measurements were performed on cholesterol, cerebrosides, sulfatides, phospholipids and sphingolipids in total lipid extracts of white matter, gray matter and cerebellar regions of human brains. No significant differences in the lipid class composition between the groups were observed.
authors.affiliation: Laboratory of Membrane Biochemistry and Biophysics, NIAAA, National Institutes of Health, MD 20852, USA.
authors.forename: N U
authors.initials: NU
authors.lastname: Olsson
authors.forename: A J
authors.initials: AJ
authors.lastname: Harding
authors.forename: C
authors.initials: C
authors.lastname: Harper
authors.forename: N
authors.initials: N
authors.lastname: Salem
mesh_terms: D000437:Alcoholism / Q000378:metabolism*; D001923:Brain Chemistry; D002554:Cerebrosides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008027:Light; D008563:Membrane Lipids / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010955:Plasmalogens / Q000032:analysis; D012542:Scattering, Radiation; D013107:Sphingolipids / Q000032:analysis; D013433:Sulfoglycosphingolipids / Q000032:analysis
chemical_list: D002554:Cerebrosides; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010955:Plasmalogens; D013107:Sphingolipids; D013433:Sulfoglycosphingolipids; D002784:Cholesterol