Determination of the cyanobacterial neurotoxin, anatoxin-a, by derivatisation using 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F) and HPLC analysis with fluorimetric detection.
A sensitive HPLC analysis has been developed for the determination of anatoxin-a in cyanobacterial bloom material and in raw and treated waters. Efficient extraction of this toxin from its matrix can be achieved using solid-phase extraction with a weak cation exchanger. HPLC analysis with fluorimetric detection (lambda ex 470 nm, lambda em 530 nm) was possible after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). This new analytical protocol permits the detection of anatoxin-a at low concentrations (0.1 micrograms/L) and gave a good linear calibration (1-20 micrograms/L, r = 0.997).
James
K J
KJ
Chemistry Department, Cork RTC, Ireland.
Sherlock
I R
IR
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D001427:Bacterial Toxins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D000458:Cyanobacteria; D000087522:Cyanobacteria Toxins; D005456:Fluorescent Dyes; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D009498:Neurotoxins / Q000032:analysis*; D014326:Tropanes
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D005456:Fluorescent Dyes; D008387:Marine Toxins; D052998:Microcystins; D009498:Neurotoxins; D014326:Tropanes; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; C509783:anatoxin a; D009327:4-Chloro-7-nitrobenzofurazan