Effect of non-saponin fraction from Panax ginseng on cGMP and thromboxane A2 in human platelet aggregation.
The non-saponin fraction (NSF; lipophilic fraction) from the roots of Panax ginseng inhibited the aggregation of human platelets induced by thrombin (0.1 units/ml) in a dose-dependent manner. NSF induced the elevation of cGMP concentration in human platelets in a similar manner to molsidomine, a known vasodilator. NSF also inhibited Ca(2+)-influx into platelets. While verapamil, a Ca(2+)-antagonist, increased the cAMP level in platelets stimulated by thrombin, NSF had little effect on cAMP formation. Instead, NSF potently inhibited the thromboxane A2 (TXA2) production. The results suggest that NSF may regulate the levels of cGMP and TXA2 to inhibit platelet aggregation induced by thrombin.
Park
H J
HJ
Department of Biochemical Pharmacology, Korea Ginseng and Tobacco Research Institute, Taejon.
Rhee
M H
MH
Park
K M
KM
Nam
K Y
KY
Park
K H
KH
D000480:Alkynes; D001792:Blood Platelets / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D006152:Cyclic GMP / Q000096:biosynthesis*; D053280:Diynes; D005233:Fatty Alcohols / Q000494:pharmacology*; D006801:Humans; D008981:Molsidomine / Q000494:pharmacology; D005894:Panax; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013917:Thrombin; D013928:Thromboxane A2 / Q000096:biosynthesis*; D014665:Vasodilator Agents / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000480:Alkynes; D002121:Calcium Channel Blockers; D053280:Diynes; D005233:Fatty Alcohols; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D014665:Vasodilator Agents; C018541:falcarinol; D013928:Thromboxane A2; D014700:Verapamil; D008981:Molsidomine; D000242:Cyclic AMP; D013917:Thrombin; D006152:Cyclic GMP; D002118:Calcium