title: An oral ivermectin regimen that eradicates pinworms (Syphacia spp.) in laboratory rats and mice.
abstract: Pinworm infection, a common problem in laboratory rodent colonies, is difficult to control because anthelmintics like ivermectin eliminate adult worms but have no effect on ova, which can survive ex vivo for prolonged periods. On the premise that repeated treatments with ivermectin would keep rodents parasite-free until all ova matured into ivermectin-susceptible worms in vivo or died in vivo or ex vivo, 80 rats and 25 mice heavily infected with pinworms (Syphacia obvelata and S. muris) were randomized to receive two to five courses of ivermectin 3 days apart or no treatment. During each treatment, ivermectin was given for 4 days in the drinking water; based on water consumption, the mean ivermectin dose was 2.9 and 4.0 mg/kg of body weight per day in rats and mice respectively. Ova production was monitored by weekly cellophane tape tests; 29 to 32 weeks after treatment ended, all rodents were euthanized, and their evacuated large intestinal contents were examined for adult pinworms and ova. Despite intermittently negative cellophane tape test results in untreated rodents (10 rats and 5 mice), all were infected with parasites at the end of the follow-up period. These findings underscore the limitations of the tape test for diagnosis of pinworm infection. After two courses of ivermectin, 1 of 10 rats and four of five mice were infected, whereas after three courses only 1 of 40 rats and one of five mice had parasites. In contrast, none of the 20 rats or 10 mice given either four or five courses of ivermectin had parasites at 30 to 32 weeks of follow-up evaluation. This simple and well-tolerated ivermectin regimen may help to treat and control pinworm infection in laboratory rodent colonies.
authors.affiliation: Hamilton Civic Hospitals Research Centre, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Klement
authors.forename: J M
authors.initials: JM
authors.lastname: Augustine
authors.forename: K H
authors.initials: KH
authors.lastname: Delaney
authors.forename: G
authors.initials: G
authors.lastname: Klement
authors.forename: J I
authors.initials: JI
authors.lastname: Weitz
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage*; D005260:Female; D007559:Ivermectin / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000469:parasitology; D010123:Oxyuriasis / Q000188:drug therapy / Q000662:veterinary*; D010124:Oxyuroidea / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains / Q000469:parasitology; D012376:Rodent Diseases / Q000188:drug therapy* / Q000517:prevention & control; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D007559:Ivermectin