title: Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.
abstract: Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.
authors.affiliation: Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: da Silva
authors.forename: D A
authors.initials: DA
authors.lastname: Schwartz
authors.forename: G S
authors.initials: GS
authors.lastname: Visvesvara
authors.forename: H
authors.initials: H
authors.lastname: de Moura
authors.forename: S B
authors.initials: SB
authors.lastname: Slemenda
authors.forename: N J
authors.initials: NJ
authors.lastname: Pieniazek
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D016816:Microsporida / Q000235:genetics* / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D016053:RNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D016053:RNA, Protozoan; D012335:RNA, Ribosomal