Perioperative analysis of vitreous cell components by immunoimpression cytology.
BACKGROUND
Perioperative analysis and classification of vitreous cell components needs a quick method sufficient to prepare both single cells and cellular membranes.


METHODS
In an attempt to clarify the usefulness of immunoimpression cytology we examined 82 eyes with proliferative vitreoretinopathy, proliferative diabetic retinopathy, perforating injuries and contusions.


RESULTS
With this method it is possible to perioperatively obtain cells from surfaces of vitreous membranes as well as single cells in suspension. After staining, various cell types could be differentiated morphologically and on the basis of antibody response to cell markers. We found single cells reacting with the antibodies 27E10 (4 positive of 14 tested), vimentin (2/12), RM 3/1 (2/15), LCA (1/ 11) and, particularly, with antiproliferating cell nuclear antigen (9/18).


CONCLUSIONS
With immunoimpression cytology it is possible to determine cell surface markers rapidly and accurately from specimens obtained at the time of vitrectomy. The clinical utility of this test will be ascertained by future studies.
Wiedemann
P
P
Augenklinik, Liebigstrasse 22b, Leipzig, Germany.
Kohlmann
H
H
D015703:Antigens, CD / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D015415:Biomarkers; D002455:Cell Division; D003930:Diabetic Retinopathy / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D005131:Eye Injuries / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D015807:Eye Injuries, Penetrating / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen / Q000276:immunology; D014746:Vimentin / Q000276:immunology; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D014822:Vitreous Body / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D014949:Wounds, Nonpenetrating / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin; C067980:CD68 antigen, human; D000097382:CD68 Molecule