High-performance liquid chromatography assay of rifapentine in human serum.
A high-performance liquid chromatographic method for the determination of rifapentine in human serum was developed. The method utilized a Spherisorb C18 column, ultraviolet detection (336 nm), rifampin as internal standard and a calibration curve (C = 7.010 As/Ain +/- 0.156, r = 0.999) with reproducibility studies which yield a coefficient of variation (C.V.) of intra-day and inter-day assays lower than 10%. The average recovery of rifapentine from serum in the concentration range of 0.5 to 30 micrograms/ml was 92.93 +/- 9.704%.
He
X
X
Department of Pathophysiology, Nanjing Railway Medical College, China.
Wang
J
J
Liu
X
X
Chen
X
X
D000995:Antitubercular Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006207:Half-Life; D006801:Humans; D015203:Reproducibility of Results; D012293:Rifampin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D000995:Antitubercular Agents; D012293:Rifampin; C018421:rifapentine