Fluorescent focus reduction assay for the screening of antiadenoviral agents.
A method for screening of antiviral compounds against adenoviruses was established. Test compounds were diluted and plated in chamber slides for tissue culture. Drug-treated, virus-infected cultures were stained with fluorescein isothiocyanate conjugated rabbit antibodies against adenovirus hexon type 2 and fluorescent cells were counted by microscopy. This assay is more sensitive than the colorimetric method and requires smaller volumes of compounds when compared with the standard method using plaque assay.
Mentel
R
R
Institut für Medizinische Mikrobiologie der Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Matthes
E
E
Janta-Lipinski
M
M
Wegner
U
U
D000260:Adenoviruses, Human / Q000187:drug effects*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans
D000998:Antiviral Agents; D016650:Fluorescein-5-isothiocyanate