Differential effects of butyrate and dibutyryl cAMP on mRNA levels of muscarinic acetylcholine receptor subtypes expressed in neurohybrid cell lines.
NCB-20 cells expressed m1- and m4-muscarinic acetylcholine receptor (mAChR) mRNAs, while NG108-15 cells expressed only m4-mAChR mRNA. Butyrate induced a time-dependent increase in the level of m1-mAChR mRNA with no change in the m4-mAChR mRNA level in NCB-20 cells. Similarly, butyrate did not affect the m4-mAChR mRNA level in NG108-15 cells. In contrast, dibutyryl cAMP caused a significant time-dependent decrease in the level of m4-mAChR mRNA in NCB-20 and NG108-15 cells as well as m1-mAChR mRNA in NCB-20 cells. Our results suggest that these two differentiating agents are important physiological regulators of the transcription and/or stability of the mRNA of certain mAChR subtypes expressed in these two neurohybrid cell lines.
Fukamauchi
F
F
Department of Molecular Medical Science, Tokyo Medical and Dental University, Japan.
Mataga
N
N
Wang
Y J
YJ
Chuang
D M
DM
D000818:Animals; D015152:Blotting, Northern; D003994:Bucladesine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D015342:DNA Probes; D015536:Down-Regulation / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D006822:Hybrid Cells / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009447:Neuroblastoma; D012333:RNA, Messenger / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
D002087:Butyrates; D015342:DNA Probes; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D020148:Butyric Acid; D003994:Bucladesine