Changes in blood cell numbers and formation of eicosanoids during clotting in warm- and cold-acclimated bullfrogs (Rana catesbeiana).
Amphibian blood cells play an important role in eicosanoid synthesis. Formation of eicosanoids during clotting of whole human blood but not frog blood has been reported. Amphibian blood cell numbers are affected by cold exposure; therefore eicosanoid production may reflect these changes. Blood cell changes during warm, and short- and long-term-cold exposure periods were recorded and the formation of leukotriene B4 (LTB4), LTC4, thromboxane (TXB2), and prostaglandin E2 (PGE2) was measured during blood clotting by using radioimmunoassay. Short-term (1 day) cold exposure (5 degrees) did not significantly decrease blood cell number or eicosanoid production during clotting. However, following exposure to cold (5 degrees) for 10 or 30 days, blood cell number and production of LTB4, TXB2, and PGE2 during clotting decreased when compared with warm acclimated controls (22 degrees). LTC4 levels during blood clotting decreased significantly only after 30 days of cold exposure. The results demonstrate that eicosanoid synthesis can be correlated with blood cell number in warm- and cold-exposed bullfrogs.
Wang
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Department of Biology, New Mexico State University, Las Cruces 88003, USA.
Herman
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C
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D001772:Blood Cell Count; D001773:Blood Cells / Q000502:physiology*; D001777:Blood Coagulation / Q000502:physiology*; D003080:Cold Temperature; D015777:Eicosanoids / Q000097:blood*; D005260:Female; D006358:Hot Temperature; D015289:Leukotrienes / Q000096:biosynthesis; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis; D011863:Radioimmunoassay; D011892:Rana catesbeiana / Q000502:physiology*; D013931:Thromboxanes / Q000096:biosynthesis
D015777:Eicosanoids; D015289:Leukotrienes; D011453:Prostaglandins; D013931:Thromboxanes