Determination of sumatriptan succinate in human plasma by high-performance liquid chromatography with coulometric detection and utilization of solid-phase extraction.
Sumatriptan succinate (the analyte) and naloxone (the internal standard) were extracted from plasma with a solid-phase extraction technique. Chromatography and detection were performed by isocratic reversed-phase high-performance liquid chromatography with coulometric end-point detection. The standard curve was linear over the range 0-100 ng/ml of sumatriptan succinate in plasma. The reproducibility (as defined by the coefficient of variation, C.V.) over the range of the standard curve was 4.9-7.3%. The recovery averaged 83%. The sensitivity was 0.25 ng of sumatriptan on column (allowing a concentration of 0.5 ng/ml to be determined from a 1-ml plasma sample volume). Plasma profiles of the analyte following subcutaneous (s.c.) administration in eight normal male volunteers, are presented.
Franklin
M
M
University of Oxford, Department of Psychiatry, Psychopharmacology Research Unit, Littlemore Hospital, Oxford, UK.
Odontiadis
J
J
Clement
E M
EM
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D009270:Naloxone; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood*; D018170:Sumatriptan / Q000008:administration & dosage / Q000097:blood*
D017366:Serotonin Receptor Agonists; D009270:Naloxone; D018170:Sumatriptan