Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate.
The DNA of a nuclear polyhedrosis virus (NPV) propagated in the larvae of S. litura was analysed with AluI, ApaI, BamHI, BglII, BstEII, BstNI, ClaI, EcoRI, EcoRV, HindIII, HinfI, KpnI, MspI, PstI, PvuII, SalI, Sau3AI, SmaI, TaqI, XbaI, and XhoI. The unique restriction endonuclease profiles of the virus indicate it to be a distinct NPV isolate. The size of the viral genome was estimated to 132 kbp; the genome was mapped with HindIII and PstI.
Das
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RH
Centre for Biochemical Technology, Delhi University, India.
Prasad
Y D
YD
D000818:Animals; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods; D016679:Genome, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D018411:Spodoptera / Q000821:virology*
D004262:DNA Restriction Enzymes