title: Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56.
abstract: The genes coding for the type II restriction-modification (R/M) system LlaBI, which recognized the sequence 5'-C decreases TRYAG-3', have been cloned from a plasmid in Lactococcus lactis subsp. cremoris W56 and sequenced. The DNA sequence predicts an endonuclease of 299 amino acids (33 kDa) and a methylase of 580 amino acids (65 kDa). A 4.0-kb HindIII fragment in pSA3 was able to restrict bacteriophages, showing that the cloned R/M system can function as a phage defense mechanism in L. lactis.
authors.affiliation: Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
authors.forename: N R
authors.initials: NR
authors.lastname: Nyengaard
authors.forename: J
authors.initials: J
authors.lastname: Falkenberg-Klok
authors.forename: J
authors.initials: J
authors.lastname: Josephsen
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D007778:Lactobacillus / Q000235:genetics / Q000821:virology*; D008969:Molecular Sequence Data
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific