Phospholipases Involved in Activation of the Neutrophil NADPH Oxidase
Stimulation of neutrophils with a variety of stimuli can result in the activation of phospholipases A2, C, or D with the resultant hydrolysis of plasma membrane phospholipids and the formation of important second messenger molecules. In the neutrophil, the activities of these phospholipases have been implicated in the processes of both stimulating and maintaining oxidase activation. In this review, some of the methods currently used to measure the products of phospholipase activation in the neutrophil are described, along with the possible role of their products in reactive oxidant production by the neutrophil NADPH oxidase.
Watson
F
Department of Biochemistry, University of Liverpool, Liverpool, L69 3BX, United Kingdom