Quantifying cell mono-layer cultures by video imaging.
A method is described in which the relative number of adherent cells in multi-well tissue-culture plates is assayed by staining the cells with Giemsa and capturing the image of the stained cells with a video camera and charged-coupled device. The resultant image is quantified using the associated video imaging software. The method is shown to be sensitive and reproducible and should be useful for studies where quantifying relative cell numbers and/or proliferation in vitro is required.
Miller
K S
KS
University of Tulsa, OK 74104, USA. millerks@centum.utulsa.edu
Hook
L A
LA
D000818:Animals; D016466:CHO Cells / Q000166:cytology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002455:Cell Division; D006224:Cricetinae; D018715:Microscopy, Video / Q000379:methods*; D013194:Staining and Labeling; D014162:Transfection