title: Spontaneous, persistent infection of a B-cell lymphoma with adenovirus.
abstract: An adenovirus culture-positive lymphoblastoid cell line was derived from a bone marrow transplant recipient with fatal B-cell lymphoproliferative disease and adenovirus pneumonia. At autopsy, focal areas of the lymphoma infiltrating the patient's lung were positive for adenovirus proteins by immunohistochemical staining. The Epstein-Barr virus-transformed B-cell line Mk, established from pleural fluid cells, contained adenovirus virions in both the nucleus and the cytoplasm by electron microscopy. The majority of Mk cells expressed adenovirus proteins and produced a high level of infectious adenovirus by plaque assay analysis. However, in contrast to the rapid cell death induced by adenovirus in other permissive cell lines, Mk was maintained stably in tissue culture for 6 months. These data indicate that adenoviral replication is not sufficient for cell lysis and confirm that adenovirus can cause persistent infection in human lymphoid cells in vivo.
authors.affiliation: Department of Medicine, Medical College of Wisconsin, John L. Doyne Hospital, Milwaukee, Wisconsin USA.
authors.forename: P
authors.initials: P
authors.lastname: Flomenberg
authors.forename: V
authors.initials: V
authors.lastname: Piaskowski
authors.forename: J
authors.initials: J
authors.lastname: Harb
authors.forename: A
authors.initials: A
authors.lastname: Segura
authors.forename: J T
authors.initials: JT
authors.lastname: Casper
mesh_terms: D000257:Adenoviridae Infections / Q000150:complications / Q000473:pathology / Q000821:virology*; D000260:Adenoviruses, Human / Q000302:isolation & purification* / Q000648:ultrastructure; D001853:Bone Marrow / Q000821:virology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002675:Child, Preschool; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016393:Lymphoma, B-Cell / Q000150:complications / Q000473:pathology / Q000821:virology*; D008297:Male; D011024:Pneumonia, Viral / Q000150:complications / Q000473:pathology / Q000821:virology*; D014407:Tumor Cells, Cultured; D017735:Virus Latency
chemical_list: D036022:Capsid Proteins; C043322:penton protein, adenovirus