Developmental pattern of NADPH-diaphorase activity and nitric oxide-stimulated cGMP immunoreactivity in the frontal rat cortex and its role in functional recovery from aspiration lesions.
The present study was undertaken to (1) explore the cortical nitric oxide (NO)-system during postnatal development and (2) to see whether or not the NO-system reacts differentially after neonatal and adult lesions of the medial prefrontal cortex. Three aspects of the NO-system were studied, i.e., NADPH-diaphorase (NADPH-d) activity, sodium nitroprusside (SNP)- and N-methyl-D-aspartate (NMDA)-stimulated cGMP-immunoreactivity (cGMP-IR). It was shown that: (1) the development of NADPH-d activity containing cells is continued in the period from P6 until P21; (2) during the same period, large developmental changes take place in basal, and SNP- or NMDA-stimulated cGMP-IR in the cortex. These changes are regionally specific and follow the general cortical developmental pattern; and (3) aspiration lesions do not induce major changes in the distribution of NADPH-d activity or cGMP-IR, either basal, SNP- or NMDA-stimulated.
van Eden
C G
CG
Netherlands Institute for Brain Research, Graduate School for Neurosciences Amsterdam, Netherlands. c.van.eden.@nih.knaw.nl
Steinbusch
H W
HW
Rinkens
A
A
de Vente
J
J
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D006152:Cyclic GMP / Q000276:immunology* / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D007150:Immunohistochemistry; D016202:N-Methylaspartate / Q000494:pharmacology; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000201:enzymology* / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000494:pharmacology
D018690:Excitatory Amino Acid Agonists; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D016202:N-Methylaspartate; D009252:NADPH Dehydrogenase; D006152:Cyclic GMP