A 20-kDa protein in human seminal plasma that is identical to gross cystic disease fluid protein 15 and prolactin-inducible protein.
A new method is described for isolating the 20-kDa protein, which used to be purified using an actin-immobilized column from human seminal plasma. This method employed ion-exchange column chromatography and isoelectric focusing separation. The level of seminal protein was determined by sandwich ELISA to be 1.06 +/- 0.27 mg/mL (mean +/- SD, n = 8). Its cDNA was cloned from a human salivary gland cDNA library by immunoscreening. The 553-nucleotide sequence included the 5' untranslated region and extended to the poly(A) tail. It encoded a protein of 118 amino acid residues in addition to a signal sequence of 28 residues. This sequence was identical to those of gross cystic disease fluid protein 15 and prolactin-inducible protein cDNAs. Northern blotting revealed the common expression in the submandibular gland and seminal vesicle. An immunohistochemical study in paraffin-embedded tissues from human male sex organs also indicated that the 20-kDa protein is mainly produced in the seminal vesicle.
Osawa
M
M
Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa, Japan.
Seto
Y
Y
Yukawa
N
N
Saito
T
T
Takeichi
S
S
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D001053:Apolipoproteins; D053399:Apolipoproteins D; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary; D006023:Glycoproteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D011388:Prolactin / Q000494:pharmacology; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000276:immunology*
C024328:APOD protein, human; D000906:Antibodies; D001053:Apolipoproteins; D053399:Apolipoproteins D; D002352:Carrier Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D026901:Membrane Transport Proteins; C488892:PIP protein, human; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D011388:Prolactin; D004247:DNA