Quantitation of tobramycin concentration by laser microscopy in an epithelial cell of renal tubule.
To estimate tobramycin concentration in an epithelial cell of renal tubule without pulverizing or extracting it, we used immunohistochemical straining, using antibody coated colloidal gold. The intensity of reflection of colloidal gold at 514nm was measured with a laser microscope. Colloidal gold, reacted with tobramycin, was determined as having a gray scale on the microscopic image. This method was useful and sensitive enough to know the local concentrations of drug and/or other chemical substances.
Kimura
H
H
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Mukaida
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D017979:Gold Colloid; D006168:Guinea Pigs; D007150:Immunohistochemistry / Q000379:methods*; D007684:Kidney Tubules / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D014031:Tobramycin / Q000493:pharmacokinetics*
D000900:Anti-Bacterial Agents; D017979:Gold Colloid; D014031:Tobramycin