Human T cells expressing an invariant V alpha 24-J alpha Q TCR alpha are CD4- and heterogeneous with respect to TCR beta expression.
A population of mature CD4-CD8-double-negative T cells that expresses an invariant Valpha24-JalphaQ TCR has been identified in humans; the majority of these cells appear to express Vbeta11. A closely related in variant TCRalpha chain is also expressed by a population of NK1+ murine T cells, but these cells may be either CD4+ or double negative. In this report, multiple CD4+ or double-negative Valpha24+Vbeta11+ T-cell clones were isolated, and only the double-negative clones were found to express the invariant TCRalpha chain. Studies of TCRbeta chains expressed by these cells demonstrate that a subset in some donors use Vbeta genes other than Vbeta11. Characterization of Vbeta11 TCRs in one donor by CDR3-length analysis was also carried out. The results indicate that multiple Vbeta11 TCRs of differing CDR3 lengths may associate with the invariant TCRalpha chain.
Porcelli
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S
Hematology-Oncology Division, Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA.
Gerdes
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D
Fertig
A M
AM
Balk
S P
SP
D015704:CD4 Antigens / Q000032:analysis*; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
D015704:CD4 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta