Identification of plexin family molecules in mice.
By screening of E17.5 mouse brain cDNA libraries, we isolated two cDNAs encoding new plexin-like proteins. Sequencing revealed that these two proteins were type 1 membrane proteins which showed over 60% identity at the amino acid level to mouse plexin 1. Moreover, putative extracellular segments of these two proteins had three repeats of a cysteine-rich domain which is a common motif for plexin proteins. Thus, we named these two proteins mouse plexin 2 and mouse plexin 3. We obtained mouse plexin 3 cDNA clones in which a part of protein-coding region was deleted. Also, Northern blot analysis showed molecular heterogeneity in mouse plexin 2 mRNAs. These findings indicate that in the mouse, plexins comprise a molecular family (the plexin family).
Kameyama
T
T
Laboratory of Developmental Biology, Nagoya University Graduate School of Science, Japan.
Murakami
Y
Y
Suto
F
F
Kawakami
A
A
Takagi
S
S
Hirata
T
T
Fujisawa
H
H
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C091529:plexin