Generation of polyketide libraries via combinatorial biosynthesis.
Polyketides are a family of structurally complex natural products that include a number of important pharmaceuticals. Motivated by the value of these natural products, there has been much research focused on developing guidelines for engineering polyketide synthases (PKSs) to generate novel polyketides. Recent studies have provided interesting insights into the enzymatic specificity of the polyketide synthesis pathway, and have demonstrated that various PKSs can be genetically manipulated to synthesize 'unnatural' polyketide natural products. In this article, we discuss the synthesis of polyketides and polyketide libraries by combinatorial biosynthesis.
Khosla
C
C
Department of Chemical Engineering, Stanford University, Stanford 94305-5025, USA. ck@chemeng.stanford.edu
Zawada
R J
RJ
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001709:Biotechnology / Q000639:trends; D015195:Drug Design; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics*; D015202:Protein Engineering
D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes