A homologue of the cysteine proteinase gene (ACP1 or Eh-CPp3) of pathogenic Entamoeba histolytica is present in non-pathogenic E. dispar strains.
One of the three cysteine proteinase genes, ACP1 (or CP 3), has been reported to be missing in non-pathogenic strains of Entamoeba histolytica (or Entamoeba dispar as recently labeled). Unexpectedly, a gene fragment very similar in its sequence (95% homology) to ACP1 of pathogenic strains was obtained by use of the polymerase chain reaction from genomic DNA and cDNA of various cloned non-pathogenic strains as well as in 23 clinical isolates from asymptomatic carriers. The finding of the ACP1 homologue in non-pathogenic or E. dispar strains rules out the proposed use of its absence for diagnostic purposes.
Mirelman
D
D
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel. bfmirelm@weizmann.weizmann.ac.il
Nuchamowitz
Y
Y
Böhm-Gloning
B
B
Walderich
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004747:Entamoeba / Q000201:enzymology* / Q000235:genetics* / Q000472:pathogenicity; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics* / Q000472:pathogenicity; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D003546:Cysteine Endopeptidases