Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine.
A study of the capillary electrophoretic separations of proteins and peptides using high-molecular-mass polyethyleneimine (PEI) is presented. Experiments were performed in the PEI-coated capillaries together with the use of this polymer as a buffer additive under different separation conditions. The effects of pH and the concentration of PEI in the buffer on the electroosmotic flow and the migration orders of biopolymers were investigated. The use of the cationic polymer offers an alternative for the modification of the separation selectivity and resolution of biopolymers.
Cifuentes
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A
Laboratory of Analytical Chemistry, University of Amsterdam, Netherlands.
Poppe
H
H
Kraak
J C
JC
Erim
F B
FB
D000595:Amino Acid Sequence; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D004798:Enzymes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry*; D009994:Osmolar Concentration; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
D002021:Buffers; D004798:Enzymes; D009842:Oligopeptides; D011108:Polymers; D011094:Polyethyleneimine