title: Cloning of a novel membrane-linked metalloproteinase from human myeloma cells.
abstract: We have isolated a novel cDNA from human myeloma cells encoding a member of the reprolysin family of metalloproteinases. Derived amino acid sequence predicts a protein of approx. 76 kDa. The open reading frame predicts the presence of a leader peptide, a pro-peptide with a 'cysteine switch', a metalloproteinase domain, a disintegrin-like domain, a cysteine-rich domain, an epidermal growth factor-like domain and a putative transmembrane sequence. Expression of the mRNA for this metalloproteinase has been demonstrated in human myeloma cells.
authors.affiliation: Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, U.K.
authors.forename: N
authors.initials: N
authors.lastname: McKie
authors.forename: D J
authors.initials: DJ
authors.lastname: Dallas
authors.forename: T
authors.initials: T
authors.lastname: Edwards
authors.forename: J F
authors.initials: JF
authors.lastname: Apperley
authors.forename: R G
authors.initials: RG
authors.lastname: Russell
authors.forename: P I
authors.initials: PI
authors.lastname: Croucher
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D017931:DNA Primers; D019483:Disintegrins / Q000737:chemistry; D005260:Female; D015723:Gene Library; D006801:Humans; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009101:Multiple Myeloma; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D019483:Disintegrins; D011994:Recombinant Proteins; D008666:Metalloendopeptidases