4-hydroxy-2,3-trans-nonenal induces transcription and expression of aldose reductase.
Treatment of A7r5 rat vascular smooth muscle cells with 4-hydroxy-2,3-trans-nonenal (HNE) resulted in increased aldose reductase mRNA transcription. Induction was time dependent, reaching a maximum (2,3-fold) after 7 hours. An enzymatically active aldose reductase analysed by Western blotting and enzyme assays was expressed. Sorbinil, an aldose reductase inhibitor, induced a significant enhancement of HNE cytotoxicity, indicating a protective role of aldose reductase against HNE-induced A7r5 cell death. These data indicate that induction of aldose reductase by HNE may represent an important cellular defence mechanism against oxidative injury.
Spycher
S
S
Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
Tabataba-Vakili
S
S
O'Donnell
V B
VB
Palomba
L
L
Azzi
A
A
D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D000447:Aldehydes; D000449:Aldehyde Reductase; C027576:4-hydroxy-2-nonenal