title: Purification and characterization of a cellulase from the giant snail Achatina fulica.
abstract: A cellulase with activity as a cellobiohydrolase [EC3.2.1.91] was purified from the intestinal juice of the giant snail Achatina fulica. The enzyme (M(r) = about 23,000, and pI = about 5.3) also had a weak beta-glucosidase activity. The amino acid sequence of the N-terminal 20 amino acids was analyzed, and no similarity was noted for sequences of other known cellobiohydrolases.
authors.affiliation: Department of Chemistry, Kyushu University, Fukuoka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Maeda
authors.forename: Y
authors.initials: Y
authors.lastname: Shimohigashi
authors.forename: H
authors.initials: H
authors.lastname: Kihara
authors.forename: M
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002480:Cellulase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007422:Intestines / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012908:Snails / Q000378:metabolism
chemical_list: D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase