Viable AC-2, a new adult bovine serum- and colostrum-based supplement for the culture of mammalian cells.
In this study we have shown that Viable AC-2, a medium based on the ultrafiltrate fraction of bovine colostrum and adult bovine serum, can be used successfully as a fetal bovine serum (FBS) substitute in the culture of several anchorage-dependent and independent cell lines. Of the 15 cell lines cultured in 8% Viable AC-2 in microplates, 10 reached the maximum cell density of 65%-123% of that in 10% FBS, 4 cell lines reached maximum cell density of 35%-65% of that in 10% FBS and only one cell line, a human osteosarcoma G-292, grew slowly in Viable AC-2. In a small-scale suspension culture, 8%-15% Viable AC-2 supports the growth of Chinese hamster ovary cells (CHO-K1) on microcarriers in spinner flasks significantly better than 10% FBS. Shionogi mouse mammary tumour cell line (S115) transfected with human alpha 2-adrenergic receptor subtype C2 was used as a model to study recombinant protein production in Viable AC-2-supplemented medium. The results showed that in cell culture flasks and in an ACUSYST-R bioreactor, the alpha 2-C2 receptor expression level per mg of total protein was similar in both Viable AC-2 and FBS.
Viander
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B
Viable Bioproducts Ltd., Turku Technology Centre, Finland.
Ala-Uotila
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S
Jalkanen
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M
Pakkanen
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R
D000818:Animals; D001798:Blood Proteins; D016466:CHO Cells / Q000737:chemistry / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002522:Chlorocebus aethiops; D003126:Colostrum; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology; D008322:Mammals; D051379:Mice; D008807:Mice, Inbred BALB C; D012516:Osteosarcoma; D011941:Receptors, Adrenergic / Q000032:analysis / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology; D002583:Uterine Cervical Neoplasms
D001798:Blood Proteins; D003470:Culture Media; D011941:Receptors, Adrenergic