title: Nucleotide sequence determination and genetic analysis of the Bacteroides plasmid, pBI143.
abstract: The nucleotide sequence and genetic organization of the Bacteroides plasmid pBI143 were determined. The plasmid was 2747 base pairs (bp) and had a G+C content of 41% (GenBank Accession No. U30316). There were two open reading frames greater than 50 codons and these were designated mobA and repA. A 56-bp inverted repeat divided pBI143 into modules with repA and mobA in separate regions. There was a marked difference in the G+C content and codon usage for the two regions; repA had 33% G+C and mobA was 44% G+C. MobA had homology to other Bacteroides mobilization proteins and RepA shared homology to a replication protein from Zymomonas mobilis plasmid pZM2. These two putative replication proteins formed a subgroup of the rolling-circle replication.proteins belonging to the pSN2 family of gram-positive plasmids. Consistent with this finding, single-stranded pBI143 DNA was detected in plasmid containing Bacteroides fragilis cultures. Availability of the pBI143 sequence allowed the elucidation of the complete nucleotide sequence for pFD288 an 8.9-kb Bacteroides shuttle vector (GenBank Accession No. U30830).
authors.affiliation: Department of Microbiology and Immunology, East Carolina University, Greenvil
authors.forename: C J
authors.initials: CJ
authors.lastname: Smith
authors.forename: L A
authors.initials: LA
authors.lastname: Rollins
authors.forename: A C
authors.initials: AC
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001439:Bacteroides / Q000235:genetics*; D001483:Base Sequence; D004265:DNA Helicases; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D011506:Proteins; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011506:Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases