title: Rapid assay of HSF1 and HSF2 gene expression by RT-PCR.
abstract: Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.
authors.affiliation: Department of Clinical Physiology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Ding
authors.forename: R C
authors.initials: RC
authors.lastname: Smallridge
authors.forename: R J
authors.initials: RJ
authors.lastname: Galloway
authors.forename: J G
authors.initials: JG
authors.lastname: Kiang
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000737:chemistry
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C070389:HSF2 protein, human