NF-kappa B transcription factor subunits in rat brain: colocalization of p65 and alpha-MSH.
The subunit proteins p50 and p65 of the transcription factor NF-kappa B inhibitory protein were immunocytochemically identified and mapped in rat brain. The p65 subunit was localized to the cytoplasm of neurons in the lateral hypothalamus and colocalized with alpha-MSH in neurons identified as the alpha-2 component of the alpha-MSH system. The p50 subunit protein was distributed throughout the neocortex, basal ganglia, thalamic, and hypothalamic nuclei, and certain nuclei of the pons and medulla. The I-kappa B protein, which is necessary for the cytoplasmic sequestration of the NF-kappa B transcription factor complex, was identified specifically in regions of limbic, hypothalamic, and autonomic nuclei.
Joseph
S A
SA
Department of Surgery, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Tassorelli
C
C
Prasad
A V
AV
Lynd-Balta
E
E
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D004027:Diencephalon / Q000166:cytology; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology; D008636:Mesencephalon / Q000166:cytology; D008970:Molecular Weight; D016328:NF-kappa B / Q000032:analysis* / Q000037:antagonists & inhibitors; D009474:Neurons / Q000166:cytology*; D009928:Organ Specificity; D011149:Pons / Q000166:cytology; D011518:Proto-Oncogene Proteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013687:Telencephalon / Q000166:cytology; D051841:Transcription Factor RelB; D014157:Transcription Factors; D000521:alpha-MSH / Q000032:analysis*
D046911:Macromolecular Substances; D016328:NF-kappa B; D011518:Proto-Oncogene Proteins; C497595:Relb protein, rat; D014157:Transcription Factors; D051841:Transcription Factor RelB; D000521:alpha-MSH