title: Synthesis and properties of DNA-PNA chimeric oligomers.
abstract: Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the PNA sections lead to duplex destabilisation.
authors.affiliation: Department of Chemistry, University of Southampton, Highfield, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Finn
authors.forename: N J
authors.initials: NJ
authors.lastname: Gibson
authors.forename: R
authors.initials: R
authors.lastname: Fallon
authors.forename: A
authors.initials: A
authors.lastname: Hamilton
authors.forename: T
authors.initials: T
authors.lastname: Brown
mesh_terms: D002625:Chemistry, Organic / Q000379:methods; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011412:Propanolamines; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011412:Propanolamines; D011409:Propylene Glycols; C063408:1-aminopropanediol