Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR.
It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The inhibitor is the host DNA itself. An acceptable value for analytical sensitivity can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.
Cogswell
F B
FB
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA.
Bantar
C E
CE
Hughes
T G
TG
Gu
Y
Y
Philipp
M T
MT
D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D008193:Lyme Disease / Q000175:diagnosis / Q000382:microbiology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology*
D017931:DNA Primers; D004269:DNA, Bacterial