title: Antisense RNA regulation of the fatB iron transport protein gene in Vibrio anguillarum.
abstract: We have recently identified an antisense RNA (RNA alpha) that regulates the expression of the fatA iron transport gene encoding the outer membrane receptor for the iron-anguibactin complex. In this work, we demonstrate that RNA alpha also inhibits the expression of fatB, which encodes a 35 kDa iron transport protein and has domains homologous to other periplasmic transport proteins. The expression of fatA and fatB is repressed under iron-rich conditions, in which RNA alpha is induced. RNA alpha is homologous to two-thirds of the coding region of fatB. By cloning RNA alpha coding sequences immediately downstream of a tet promoter, we were able to obtain constitutive expression of the antisense RNA. The cloned region contains approximately 83% of the 650 nucleotide RNA alpha and is complementary to only 51% of the fatB mRNA but is still capable of causing a repression of the expression of the fatB gene. Our results in this work demonstrate that RNA alpha probably affects the stability of the fatB-specific mRNA.
authors.affiliation: Department of Molecular Microbiology and Immunology L220, School of Medicine, Oregon Health Sciences University, Portland 97201-3098, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Waldbeser
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: J H
authors.initials: JH
authors.lastname: Crosa
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D015347:RNA Probes; D016372:RNA, Antisense / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012293:Rifampin / Q000494:pharmacology; D014733:Vibrio / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C071991:FatB protein, Vibrio anguillarum; D026901:Membrane Transport Proteins; D015347:RNA Probes; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012333:RNA, Messenger; D007501:Iron; D012293:Rifampin