Fluorescence study on ligand induced conformational changes of human erythrocyte glucose transporter.
The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
Xie
W S
WS
National Laboratory of Biomacromolecules, Academia Sinica, Beijing, China.
Yuc
J C
JC
Tu
Y P
YP
D004912:Erythrocytes / Q000378:metabolism*; D005453:Fluorescence; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008024:Ligands; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects
D008024:Ligands; D009004:Monosaccharide Transport Proteins; D005947:Glucose