title: Characterization of extracellular proteases from Trametes trogii.
abstract: The peptidase activities excreted in culture broths of Trametes trogii mycelium have been identified by determining the digestion pathway of various peptides. Insulin beta-chain (30 residues), procasomorphin (10 residues) and two peptides of five residues (proctolin and thymosin alpha 1 fragment 23-27) were utilized as model substrates. Aminopeptidase, carboxypeptidase, endopeptidase and dipeptidyl aminopeptidase activities were revealed and information on their specificity was deduced. Preliminary data on the pH-dependent activity of the peptidases were also obtained by sequence analysis of the fragment mixtures produced at different pH values.
authors.affiliation: Dipartimento di Agrobiologia od Agrochimica, Universita' della Tuscia, Viterbo, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Caporale
authors.forename: A M
authors.initials: AM
authors.lastname: Garzillo
authors.forename: C
authors.initials: C
authors.lastname: Caruso
authors.forename: V
authors.initials: V
authors.lastname: Buonocore
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D010450:Endopeptidases / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011124:Polyporaceae / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D010455:Peptides; D010450:Endopeptidases; D014357:Trypsin