title: In vitro metabolism of LVV-hemorphin-7 in human plasma studied by reversed-phase high-performance liquid chromatography and micro-electrospray mass spectrometry.
abstract: In these experiments we have studied the in vitro metabolism of LVV-hemorphin-7 in human plasma by using reversed-phase high-performance liquid chromatography (RP-HPLC) in combination with micro-electrospray mass spectrometry (micro-ES-MS). Tandem mass spectrometry (MS-MS) was performed in order to verify the structure of the peptide fragments found. Incubations were performed with and without different protease inhibitors. Results showed that LVV-hemorphin-7 was metabolized from the N-terminal end of the peptide, probably by an amastatin-sensitive exopeptidase.
authors.affiliation: Department of Pharmaceutical Biosciences, Uppsala University, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Sanderson
authors.forename: P E
authors.initials: PE
authors.lastname: Andrén
authors.forename: R M
authors.initials: RM
authors.lastname: Caprioli
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002216:Captopril / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006020:Glycopeptides / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D018847:Opioid Peptides / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides; D010949:Plasma / Q000201:enzymology*; D011480:Protease Inhibitors / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D004723:Endorphins; D006020:Glycopeptides; D006454:Hemoglobins; D018847:Opioid Peptides; D010446:Peptide Fragments; D010455:Peptides; D011480:Protease Inhibitors; C070563:hemorphin 6; C049857:hemorphin 4; C085122:hemorphin 7; C016977:amastatin; C074309:LVV-hemorphin-7; D002216:Captopril; C008890:phosphoramidon