Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins.
Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.
Mingarro
I
I
Department of Biochemistry, Stockholm University, Sweden.
Whitley
P
P
Lemmon
M A
MA
von Heijne
G
G
D000409:Alanine / Q000235:genetics*; D006021:Glycophorins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D006021:Glycophorins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D000409:Alanine