title: Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas' disease described by Carlos Chagas in 1909.
abstract: Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas' disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.
authors.affiliation: Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal, de Ouro Preto, MG, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: de Lana
authors.forename: C A
authors.initials: CA
authors.lastname: Chiari
authors.forename: E
authors.initials: E
authors.lastname: Chiari
authors.forename: C M
authors.initials: CM
authors.lastname: Morel
authors.forename: A M
authors.initials: AM
authors.lastname: Gonçalves
authors.forename: A J
authors.initials: AJ
authors.lastname: Romanha
mesh_terms: D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D003470:Culture Media; D018105:DNA, Kinetoplast; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008875:Middle Aged; D015800:Protozoan Proteins / Q000032:analysis; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D003470:Culture Media; D018105:DNA, Kinetoplast; D007527:Isoenzymes; D015800:Protozoan Proteins