title: Enhancement of lymphokine-activated killer cell cytotoxicity implicated in the increased expression of surface adhesion molecules on tumor cells treated with anticancer agents.
abstract: We investigated mechanisms underlying lymphokine-activated killer (LAK) cell cytotoxicity in terms of intensity of expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte-function-associated antigen-3 (LFA-3) on Daudi and KATO-III cells treated with cis-diamminedichloroplatinum (II) (CDDP) and mitomycin-C (MMC). Enhancement (mean, 49.8%) of ICAM-1 or LFA-3 in mRNA and protein expression on treated tumor cells was found by flow cytometry, slot-blot RNA analysis, and reverse transcription-polymerase chain reaction (RT-PCR). Increases in adhesion and cytotoxicity of LAK cells to treated tumor cells were 10.1% and 17.7%, respectively. Results suggested that increased ICAM-1 or LFA-3 expression by low-dose CDDP or MMC binds LAK cells to tumor cells, helping to kill tumor cells. Thus, LAK cell therapy with anticancer agent pretreatment could be useful for treatment of cancer patients.
authors.affiliation: Department of Internal Medicine II, Fukushima Medical College, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ishihata
authors.forename: Y
authors.initials: Y
authors.lastname: Ejiri
authors.forename: M
authors.initials: M
authors.lastname: Okubo
authors.forename: M
authors.initials: M
authors.lastname: Ohara
authors.forename: R
authors.initials: R
authors.lastname: Kasukawa
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D018968:CD58 Antigens / Q000032:analysis*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D016219:Immunotherapy, Adoptive; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D018968:CD58 Antigens; D018799:Intercellular Adhesion Molecule-1