title: Translocation of diacylglycerol kinase alpha to the nuclear matrix of rat thymocytes and peripheral T-lymphocytes.
abstract: The cytosolic alpha-diacylglycerol kinase (DGK) was translocated to and tightly associated with the nuclear matrix when rat thymocytes and peripheral T-lymphocytes were stimulated with concanavalin A or anti-T-cell receptor antibody. This translocation occurred rather slowly and was completed in 3-4 h after cell stimulation. We also detected significant accumulation of nuclear phosphatidic acid interpreted as being formed by the translocated enzyme. The enzyme translocation is not directly linked to phosphoinositide turnover and protein phosphorylation, since phorbol myristate acetate and calcium ionophore did not affect the cellular DGK alpha and since we detected no covalent modification of the enzyme molecule. Although the mechanisms underlying the enzyme translocation remain unknown, our results indicate that DGK alpha participates in nuclear phospholipid metabolism occurring at the intermediate stage of lymphocyte activation.
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Kai
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: F
authors.initials: F
authors.lastname: Sakane
authors.forename: H
authors.initials: H
authors.lastname: Kanoh
mesh_terms: D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000378:metabolism; D019852:Diacylglycerol Kinase; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D015530:Nuclear Matrix / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010712:Phosphatidic Acids; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; D003850:Deoxyribonuclease I