title: Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1).
abstract: We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV-approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI-1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.
authors.affiliation: Department of Radiation Oncology, University of California, San Francisco, California, 94143, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Brzoska
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: N A
authors.initials: NA
authors.lastname: Levin
authors.forename: W L
authors.initials: WL
authors.lastname: Kuo
authors.forename: C
authors.initials: C
authors.lastname: Collins
authors.forename: K K
authors.initials: KK
authors.lastname: Fu
authors.forename: J W
authors.initials: JW
authors.lastname: Gray
authors.forename: M F
authors.initials: MF
authors.lastname: Christman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D003599:Cytoskeleton / Q000737:chemistry; D004791:Enzyme Inhibitors; D005347:Fibroblasts; D005796:Genes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D004791:Enzyme Inhibitors; C063306:HINT1 protein, human; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D011493:Protein Kinase C