title: Overproduction of recombinant ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus sp. strain PCC6301 in glucose-controlled high-cell-density fermentations by Escherichia coli K-12.
abstract: A predictive and feedback glucose feed controller, previously developed for nutrient-sufficient growth of Escherichia coli to high cell densities, was used to produce large quantities of a heterologous, cyanobacterial recombinant hexadecameric (L8S8) protein, ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) in E. coli. Culture and plasmid stability conditions were optimized to yield the production of approximately 1 g of soluble, active recombinant RubisCO per liter. Recombinant RubisCO also was produced in lactose-induced high-cell-density fermentation of E. coli K-12.
authors.affiliation: Department of Microbiology, Ohio State University, Columbus 43210, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Kleman
authors.forename: K M
authors.initials: KM
authors.lastname: Horken
authors.forename: F R
authors.initials: FR
authors.lastname: Tabita
authors.forename: W R
authors.initials: WR
authors.lastname: Strohl
mesh_terms: D000458:Cyanobacteria / Q000201:enzymology*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology*; D007785:Lactose / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase; D005947:Glucose; D007785:Lactose