title: Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones.
abstract: We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.
authors.affiliation: Laboratorie de Biochimie et Biologie Moléculaire, Université de Bordeaux II, France.
authors.forename: J
authors.initials: J
authors.lastname: Petit
authors.forename: P
authors.initials: P
authors.lastname: Boisseau
authors.forename: K
authors.initials: K
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Christie
authors.forename: C
authors.initials: C
authors.lastname: Gosden
authors.forename: W
authors.initials: W
authors.lastname: Muir
authors.forename: D
authors.initials: D
authors.lastname: St Clair
authors.forename: D
authors.initials: D
authors.lastname: Porteous
authors.forename: B
authors.initials: B
authors.lastname: Arveiler
mesh_terms: D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004251:DNA Transposable Elements; D015723:Gene Library; D005820:Genetic Testing; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements