A simple procedure for isolation of cloning vectors and endogenous plasmids from viridans group streptococci and Staphylococcus aureus.
Isolation of plasmid DNA from viridans group streptococci is difficult, and preparations are often heavily contaminated with chromosomal DNA. We developed a simple protocol to isolate pure plasmid DNA for use in different molecular techniques, including automated sequencing. The protocol is also applicable for plasmid isolation from Staphylococcus aureus. In addition, the protocol allows isolation of pure endogenous plasmids from streptococci and S. aureus.
Vriesema
A J
AJ
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
Zaat
S A
SA
Dankert
J
J
D003001:Cloning, Molecular; D005822:Genetic Vectors; D010957:Plasmids; D013211:Staphylococcus aureus / Q000235:genetics*; D013291:Streptococcus / Q000235:genetics*