title: Human papillomavirus type 16 E6 protein up-regulates the expression of the high mobility group protein HMG-I(Y) gene in mouse 10T1/2 cells.
abstract: Using a differential hybridization technique, we have identified a mouse cellular gene, high mobility group protein HMG-I(Y), whose expression is up-regulated by the E6 protein of human papillomavirus (HPV) type 16. This gene was overexpressed in E6-expressing mouse 10T1/2 cells, but not in G418-resistant 10T1/2 cells. The expression of the HMG-I(Y) gene was up-regulated by the transient expression of E6 from a zinc-inducible human metallothionein-IIA gene promoter. Expression was found to be more efficient at a confluent cell density than at a subconfluent cell density. The up-regulation of HMG-I(Y) gene expression by E6, in particular at a confluent cell density, may be part of an altered genetic program in host cells infected with HPV-16.
authors.affiliation: Department of Microbiology, Chiba University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
authors.forename: H
authors.initials: H
authors.lastname: Shirasawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shino
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Moriya
authors.forename: B
authors.initials: B
authors.lastname: Simizu
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D015854:Up-Regulation
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D006609:High Mobility Group Proteins; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D025741:HMGA1a Protein