Disaccharide analysis of chondroitin sulfate in peri-implant sulcus fluid from dental implants.
We collected peri-implant sulcus fluid by capillary tubes from sites around titanium osseointegrated implants and determined the chondroitin sulfate released into the peri-implant sulcus fluid by high-performance liquid chromatography. Chondroitin sulfate was found in all peri-implant sulcus fluid samples, and its content was similar to that in gingival crevicular fluid obtained around natural teeth. The predominant unsaturated disaccharide isomer was delta Di-0S, followed by delta Di-4S. Delta Di-6S was present in trace amounts. The amount of delta Di-0S was greater in peri-implant sulcus fluid than in gingival crevicular fluid. Assaying chondroitin sulfate disaccharides in peri-implant sulcus fluid may be an effective method of monitoring the peri-implant condition of dental implants.
Okazaki
J
J
First Department of Prosthodontics, Osaka Dental University, Japan. QWC02076@niftyserve.or.jp
Gonda
Y
Y
Kamada
A
A
Sakaki
T
T
Kitayama
N
N
Kawamura
T
T
Ueda
M
M
D002809:Chondroitin Sulfates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015921:Dental Implants; D004187:Disaccharides / Q000032:analysis; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged
D015921:Dental Implants; D004187:Disaccharides; D002809:Chondroitin Sulfates