title: Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.
abstract: Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Peña
authors.forename: J E
authors.initials: JE
authors.lastname: Stoner
authors.forename: G F
authors.initials: GF
authors.lastname: Hatfull
mesh_terms: D000926:Anticodon; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009160:Mycobacteriophages / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic; D016662:Virus Integration / Q000235:genetics*
chemical_list: D000926:Anticodon; D004269:DNA, Bacterial; D012343:RNA, Transfer