title: Agarose gel analysis of 15-40-kb PCR amplimers.
abstract: Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.
authors.affiliation: FMC BioProducts, Rockland, ME, USA. valerie_burland@fmc.com
authors.forename: V
authors.initials: V
authors.lastname: Burland
authors.forename: F P
authors.initials: FP
authors.lastname: Curtis
authors.forename: N
authors.initials: N
authors.lastname: Kusukawa
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D008274:Magnesium / Q000008:administration & dosage; D010781:Photography; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral; D008274:Magnesium