Thyroperoxidase microsatellite polymorphism in thyroid diseases.
Autoimmune thyroid diseases (AITD) cluster in families, although the nature of this phenomenon is still poorly understood. One possible approach to the identification of genetic factors contributing to the pathogenesis of AITD is the study of association between polymorphic markers and AITD themselves. In the present study we have analyzed the allelic distribution of sRA-1, a TPO tetranucleotide repeat, among patients with AITD, in comparison with patients with nonautoimmune thyroid diseases and the general population. The polymorphic marker was analyzed by PCR followed by electrophoresis on polyacrylamide denaturant gel. Our data show that no association exists between AITD and any of sRA-1 alleles, despite the important role that TPO plays as a thyroid autoantigen.
Pirro
M T
MT
Research Laboratory, IRCCS-Casa Sollievo della Sofferenza, Rotondo, Italy.
De Filippis
V
V
Di Cerbo
A
A
Scillitani
A
A
Liuzzi
A
A
Tassi
V
V
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006111:Graves Disease / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013959:Thyroid Diseases / Q000235:genetics* / Q000378:metabolism*; D013967:Thyroiditis, Autoimmune / Q000235:genetics / Q000378:metabolism
D004247:DNA; D007453:Iodide Peroxidase