title: Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.
abstract: The characterization of a new mAb, named 2F4/11, specific for porcine myelomonocytic cells is described. This mAb immunoprecipitates a non-covalently linked heterodimer of 155,000/95,000, which is expressed by granulocytes, monocytes and tissue macrophages but not by lymphocytes, erythrocytes or platelets. Immunoblot analysis localizes the 2F4/11 epitope on the largest subunit of the heterodimer. Mab 2F4/11 is able to block phagocytosis of complement-opsonized zymosan particles by PMN granulocytes and alveolar macrophages, as well as adherence to plastic surfaces of PMA-activated PMN. Together, these results suggest that mAb 2F4/11 recognizes the CD11b or alpha chain of the porcine complement type 3 receptor (CR3).
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Bullido
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: M
authors.initials: M
authors.lastname: Gómez del Moral
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: B
authors.initials: B
authors.lastname: Alvarez
authors.lastname: Ortuño
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D003167:Complement Activation; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D009928:Organ Specificity; D010587:Phagocytosis / Q000276:immunology; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D018821:CD18 Antigens