title: Characterization and cellular distribution of heat-shock proteins HSP70 and HSP60 in Trypanosoma cruzi.
abstract: At least eight protein members of HSP70 and three of the HSP60 families have been identified in Trypanosoma cruzi; of these, five HSP70 isoforms and one HSP60 isoform were respectively induced by a 2-hr heat-shock treatment at 37 degrees C. Immunoelectronmicroscopy of epimastigote, spheromastigote, and metacyclic cells obtained in vitro showed anti-HSP60 reactive proteins in the mitochondria and near the kinetoplast. Anti-HSP70 reactive proteins presented a more complex pattern. They were observed in different cellular compartments, and after heat shock all the three cell forms analyzed presented gold particles associated with the cellular membrane.
authors.affiliation: Instituto de Biofisica Carlos Chagas Filho, CCS, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Giambiagi-deMarval
authors.forename: T
authors.initials: T
authors.lastname: Souto-Padrón
authors.forename: E
authors.initials: E
authors.lastname: Rondinelli
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000032:analysis* / Q000737:chemistry; D018105:DNA, Kinetoplast / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000737:chemistry; D018869:Heat-Shock Response / Q000502:physiology; D007526:Isoelectric Point; D008928:Mitochondria / Q000737:chemistry; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000254:growth & development
chemical_list: D018834:Chaperonin 60; D018105:DNA, Kinetoplast; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins