Plasma prolactin, cortisol, and thyroid responses of the brown trout (Salmo trutta) exposed to lethal and sublethal aluminium in acidic soft waters.
Brown trout, with indwelling dorsal aortic cannulae, were exposed to various concentrations of aluminium (Al; 50 micrograms liter-1, 100% mortality over 48 hr; 25 micrograms liter-1, 50% mortality over 120 hr; 12.5 micrograms liter-1, 0% mortality over 120 hr) in acidic (pH 5.0) soft water. The plasma concentrations of prolactin (PRL), cortisol, thyroxine (T4), and triiodothyronine (T3) were monitored. Plasma PRL concentrations were transiently depressed (to less than 20% of resting concentrations) after 12 hr in trout in the two highest water Al concentrations, but were unchanged in the trout exposed to 12.5 micrograms liter-1 Al. Plasma cortisol concentrations were elevated in response to all water Al levels and remained elevated in trout in the lethal conditions. The sublethally exposed trout showed a recovery in plasma cortisol concentrations by 120 hr. Plasma T4 concentrations were significantly elevated in trout exposed to both the lethal and the sublethal Al concentrations (from mean resting concentrations of 1-2 ng ml-1 to peaks of 8.9 and 9.0 ng ml-1 in the 50 and 12.5 micrograms liter-1 Al groups, respectively), although a recovery in plasma concentrations was evident in the sublethally exposed trout from 72 hr onwards. Plasma T3 concentrations were relatively stable in the trout exposed to the two highest doses of Al, whereas the trout under the lowest, sublethal, Al conditions exhibited a sustained (12-72 hr) elevation in plasma T3 concentrations (from a mean resting concentration of 0.9 ng ml-1 to a peak of 4.2 ng ml-1 at 48 hr). No clear relationship was apparent between the plasma PRL concentrations and the previously reported ionoregulatory status of the trout.
Waring
C P
CP
Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Devon, United Kingdom.
Brown
J A
JA
Collins
J E
JE
Prunet
P
P
D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D001011:Aorta; D002404:Catheterization / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005260:Female; D005618:Fresh Water; D006854:Hydrocortisone / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D008297:Male; D011388:Prolactin / Q000097:blood*; D013961:Thyroid Gland / Q000187:drug effects* / Q000502:physiology; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D014337:Trout / Q000097:blood / Q000502:physiology*
D014284:Triiodothyronine; D011388:Prolactin; D000535:Aluminum; D013974:Thyroxine; D006854:Hydrocortisone