title: Genomic organization and sequence conservation in type I Trichomonas vaginalis viruses.
abstract: To reveal the genetic conservation of type I Trichomonas vaginalis viruses (TVV) we cloned and sequenced the 4.6-kb ds RNA of a TVV-T5 isolate for comparison with the cDNA sequence of a related TVV-T1 ds RNA. Analogous to TVV-T1, the TVV-T5 ds RNA also contains an upstream capsid protein gene overlapped with a downstream RNA-dependent RNA polymerase (RDRP) gene by a +1 reading frame shift. A conserved ribosomal slippage heptamer (C CUU UUU) was found within the consensus 14-nt overlap, and the context of the sequence surrounding the heptamer suggests a potential ribosomal frameshifting in the biosynthesis of RDRP from the initiation of capsid protein either through two consecutive -1 shifts or a +1 shift.
authors.affiliation: Division of infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: H M
authors.initials: HM
authors.lastname: Su
authors.forename: J H
authors.initials: JH
authors.lastname: Tai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D017124:Conserved Sequence; D005807:Genes, Overlapping; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014246:Trichomonas vaginalis / Q000821:virology*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase