title: Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis.
abstract: Large-volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.
authors.affiliation: School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, Northern Ireland, UK.
authors.forename: G
authors.initials: G
authors.lastname: McGrath
authors.forename: W F
authors.initials: WF
authors.lastname: Smyth
mesh_terms: D014151:Anti-Anxiety Agents / Q000032:analysis / Q000737:chemistry; D000996:Antitussive Agents / Q000032:analysis / Q000737:chemistry; D001993:Bronchodilator Agents / Q000032:analysis / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002624:Chemistry, Clinical / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis / Q000737:chemistry; D003061:Codeine / Q000032:analysis / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005479:Flurazepam / Q000032:analysis / Q000737:chemistry; D005554:Forensic Medicine / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008614:Meperidine / Q000032:analysis / Q000737:chemistry; D009294:Narcotics / Q000032:analysis / Q000737:chemistry; D009665:Noscapine / Q000032:analysis / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D013997:Time Factors
chemical_list: D014151:Anti-Anxiety Agents; D000996:Antitussive Agents; D001993:Bronchodilator Agents; D002593:Cetrimonium Compounds; D003902:Detergents; D009294:Narcotics; D004364:Pharmaceutical Preparations; D009665:Noscapine; D008614:Meperidine; D005479:Flurazepam; D003061:Codeine; D002976:Clenbuterol; D000077286:Cetrimonium