The structural origin of the color differences in the bioluminescence of firefly luciferase.
Six chimeric mutants between Hotaria parvula (lambda max = 568 nm) and Pyrocoelia miyako (lambda max = 550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light-emitting color, five chimeric luciferases could be divided into two groups: the three green-emitting mutants, classified as P. miyako luciferase, and the two yellow-emitting mutants, classified as H. parvula luciferase. Their common fragments between Val-209 and Ala-318 within each group contain the active site for the color differences.
Ohmiya
Y
Y
PRESTO, JRDC, Institute of Physical and Chemical Research (RIKEN), Wako, Japan. yohmiya@postman.riken.go.jp
Hirano
T
T
Ohashi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D003116:Color; D018076:DNA, Complementary; D008156:Luciferases / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D049449:Luminescence; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013050:Spectrometry, Fluorescence
D018076:DNA, Complementary; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D008156:Luciferases