Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22-p21 tumour-suppressor region by two-colour fluorescence in situ hybridization.
A Noti-linking clone NL1-210 (D3S1656) that contains the human MAP kinase activated protein kinase (3PK) gene was localized to 3p21.2 on DAPI-banded and propidium iodide (R-bands)-stained chromosomes by fluorescence in situ hybridization (FISH). For more precise localization of 3PK, two cosmid probes were used as a frame. In order to establish this frame, two Noti-linking clones, NL2-008 (D3S1648) and NL3-003 (D3S3872) were used to screen the cosmid library for locus extension. They mapped to 3p21 and were found to belong to two separate contigs of Noti-jumping and linking clones. Using FISH on DAPI-banded metaphase chromosomes, we have determined the precise localization of cosNL2-008 and cosNL3-003 to 3p21.2-p21.1 and 3p22-p21.3 respectively. The 3PK gene was localized to the 3p21.2 region within this frame by two-colour FISH. The orientation of the probes are tel-D3S3872-3PK-D3S1648-cen.
Szeles
A
A
Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, Stockholm, Sweden.
Bajalica-Lagercrantz
S
S
Lindblom
A
A
Lushnikova
T
T
Kashuba
V I
VI
Imreh
S
S
Nordenskjöld
M
M
Klein
G
G
Zabarovsky
E R
ER
D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003360:Cosmids; D015342:DNA Probes; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D010957:Plasmids; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D015342:DNA Probes; D047908:Intracellular Signaling Peptides and Proteins; C098416:MAP-kinase-activated kinase 3; D017346:Protein Serine-Threonine Kinases