Sulfonamide resistance gene in a transferable R plasmid of Pasteurella piscicida.
The sulfonamide resistance (SAr) determinant was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence was determined. The resistance gene (pp-sul) was localized to an approximately 1-kb region that includes the PstI-EcoRI site in the restriction map. An open reading frame coding a sul II-type gene composed of 810 nucleotides was identified. A direct repeat sequence was shown in the 5' flanking region of pp-sul, and a plasmid recombinational event may have occurred during the construction of pSP9351. In the 3' flanking region of the gene, a sequence homologous to the 5' noncoding sequence of the trimethoprim resistance gene, dhfr IX was found.
Kim
E H
EH
Chongpyong Inland Fisheries Research Laboratory, National Fisheries Research & Development Agency, Kyeonggi-do, Korea.
Aoki
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008969:Molecular Sequence Data; D016979:Pasteurella multocida / Q000235:genetics*; D011815:R Factors / Q000235:genetics*; D013449:Sulfonamides / Q000494:pharmacology*
D013449:Sulfonamides