Image cytometry analysis of Feulgen-stained nuclei in 72 lipomatous lesions including atypical lipomas and well-differentiated liposarcomas.
Well-differentiated lipomatous tumors constitute a histopathologic category whose nomenclature has been controversial, particularly with respect to the distinction between atypical lipomas of the extremities and well-differentiated liposarcomas of the retroperitoneum. To determine whether there were differences in image analytic parameters between these neoplasms, 72 lesions including 21 typical lipomas, 7 atypical lipomas, 16 retroperitoneal and 5 nonretroperitoneal well-differentiated, 9 dedifferentiated, and 14 pleomorphic liposarcomas were submitted to the computer-assisted microscopic analysis of Feulgen-stained nuclei. This methodology enabled four groups of variables to be calculated. These included: (1) quantitative chromatin pattern description (14 variables); (2) the measurement of proliferative activity (1 variable); (3) nuclear DNA content (DNA ploidy level, 5 variables); and (4) the measurement of cell density and topographical cell nuclei organization (2 variables). The results strongly suggest that atypical lipomas, whether superficial or deep, and well-differentiated liposarcomas, whether retroperitoneal or not, belong to the same category in terms of the variables analyzed.
Berthe
J V
JV
Laboratory of Histology, Faculty of Medicine, Erasmus University Hospital, Brussels, Belgium.
Goldschmidt
D
D
Salmon
I
I
Decaestecker
C
C
Remmelink
M
M
Petein
M
M
Pasteels
J L
JL
Roels
H
H
Frierson
H
H
Kiss
R
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D002455:Cell Division; D002843:Chromatin / Q000648:ultrastructure*; D004396:Coloring Agents; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D019044:Image Cytometry; D008067:Lipoma / Q000235:genetics / Q000648:ultrastructure*; D008080:Liposarcoma / Q000235:genetics / Q000648:ultrastructure*; D008875:Middle Aged; D011003:Ploidies; D012186:Retroperitoneal Neoplasms / Q000235:genetics / Q000648:ultrastructure*; D012394:Rosaniline Dyes
D002843:Chromatin; D004396:Coloring Agents; D004273:DNA, Neoplasm; C007722:Feulgen stain; D012394:Rosaniline Dyes