Genes in a 220-kb region spanning the TNF cluster in human MHC.
A search for new genes was performed in a 220-kb region around the tumor necrosis factor gene cluster in the human central major histocompatibility complex region using a cDNA hybridization and selection method. In addition to the seven known genes in this region, we identified a new gene that is preferentially expressed in spleen. We also identified two pseudogenes that have high degrees of homology to cytokeratin and cyclophillin, respectively. Expressed sequences for a human homologue of the mouse B144 gene were also found in the current analysis. RT-PCR analysis showed that B144 is expressed in spleen, in thymus, and prominently in the macrophage cell line, U937. We also independently identified the BAT1 gene to be the well-conserved homologue of a previously described rat liver nuclear protein.
Nalabolu
S R
SR
Boyer Center for Molecular Medicine, New Haven, Connecticut 06536-0812, USA.
Shukla
H
H
Nallur
G
G
Parimoo
S
S
Weissman
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D005805:Genes, MHC Class I; D015894:Genome, Human; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007633:Keratins / Q000235:genetics; D008233:Lymphotoxin-alpha / Q000235:genetics; D053308:Lymphotoxin-beta; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics; D051841:Transcription Factor RelB; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D001798:Blood Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C093604:LST1 protein, human; C505886:LTB protein, human; C490562:Lst1 protein, mouse; C505887:Ltb protein, mouse; C505888:Ltb protein, rat; D008233:Lymphotoxin-alpha; D053308:Lymphotoxin-beta; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C497593:RELB protein, human; D011971:Receptors, Immunologic; C497594:Relb protein, mouse; C497595:Relb protein, rat; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; C028752:cyclosporin receptor; D051841:Transcription Factor RelB; D007633:Keratins