A novel cytopathic microtitre plate assay for hepatitis A virus and anti-hepatitis A neutralizing antibodies.
The slow growth of hepatitis A virus (HAV) has made tissue culture assay for infectious virus difficult. Strains of the virus of greater cytopathogenicity have been selected for use in plaque cytopathic assays. However, in our hands, this assay has been difficult to reproduce consistently due to problems in maintaining intact cell monolayers over the long incubations involved. This report describes the development of a cytopathic TCID50 assay for HAV. From the results of repeated assay of one preparation of the virus, the coefficient of variation of the assay was calculated to be 4%. This assay has also been adapted to quantitate antibodies to HAV. Initial results of assaying the WHO standard immune serum globulin are comparable with the titre obtained by the radioimmunofocus inhibition test. Antibody titres in human and mouse sera could also be quantitated. The cytopathic TCID50 assay and the adapted inhibition assay described, may prove useful for the development and control of HAV vaccines and the validation of viral inactivation protocols.
Beales
L P
LP
National Institute for Biological Standards and Control, Potters Bar, Herts, UK.
Wood
D J
DJ
Minor
P D
PD
Saldanha
J A
JA
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000276:immunology*; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification*; D009500:Neutralization Tests; D015203:Reproducibility of Results
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies