title: Induction of systemic and mucosal immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gD.
abstract: We generated both replication-incompetent (HAd5-gD-E1 and HAd5-tgD-E1) and replication-competent (HAd5-gD-E3 and HAd5-tgD-E3) human adenovirus type 5 (HAd5) recombinants expressing the full (gD) or truncated form (tgD) of the glycoprotein gD gene of bovine herpevirus type 1 (BHV-1). Recombinant gD and tgD expressed by HAd5-gD-E1 and HAd5-gD-E3 and by HAd5-tgD-E1 and HAd5-tgD-E3, respectively, were recognized by gD-specific monoclonal antibodies (MAbs) directed against linear and conformational epitopes, suggesting that antigenicity of recombinant gD and tgD was similar to that of the native gD expressed in BHV-1 infected cells. In HAd5-gD-E1- or HAd5-gD-E3-inoculated cotton rats there was a strong gD- and HAd5-specific IgG and IgA antibody response. The immune response was significantly lower in animals similarly immunized with HAd5-tgD-E1 or HAd5-tgD-E3, indicating that live adenovirus vaccine vectors may be better suited to the full-length form of glycoprotein gD than its truncated form. After a BHV-1 challenge, no infectious BHV-1 virions were isolated from the trachea of cotton rats previously immunized with HAd5-gD-E1 or HAd5-gD-E3. These results suggest that adenovirus E1 insertion (replication-incompetent) and E3 insertion (replication-competent) vectors have excellent potential for use in developing live recombinant virus vaccines and provide evidence that the cotton rat model can be used in BHV-1 vaccination-challenge trials.
authors.affiliation: Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907-1243, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Mittal
authors.forename: Z
authors.initials: Z
authors.lastname: Papp
authors.forename: S K
authors.initials: SK
authors.lastname: Tikoo
authors.forename: M E
authors.initials: ME
authors.lastname: Baca-Estrada
authors.forename: D
authors.initials: D
authors.lastname: Yoo
authors.forename: M
authors.initials: M
authors.lastname: Benko
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D005822:Genetic Vectors / Q000276:immunology; D006566:Herpesviridae Infections / Q000517:prevention & control*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007114:Immunization; D011233:Precipitin Tests; D016205:Sigmodontinae; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins