Incubation time for feline immunodeficiency virus cultures.
In a recent report, Fiscus et al. (S. A. Fiscus, S. L. Welles, S. A. Spector, and J. L. Lathey, J. Clin. Microbiol. 33:246-247, 1995) have shown that qualitative human immunodeficiency virus cultures can be terminated at day 21 with minimal false-negative results. We have evaluated a large number of qualitative and quantitative feline immunodeficiency virus (FIV) isolations to determine how long FIV cultures should be incubated to obtain reasonably certain results. The rate at which FIV cultures became positive was influenced by whether the cats under study were naturally or experimentally infected, the duration of in vivo infection, and the number of infected peripheral blood mononuclear cells seeded. The results show that cultures for FIV isolation should be kept for 5 to 6 weeks.
Giannecchini
S
S
Department of Biomedicine, University of Pisa, Italy.
Matteucci
D
D
Mazzetti
P
P
Bendinelli
M
M
D000818:Animals; D002415:Cats; D005188:False Negative Reactions; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*