Advanced glycosylation end products stimulate the growth but inhibit the prostacyclin-producing ability of endothelial cells through interactions with their receptors.
The influence of advanced glycosylation end products (AGE) on endothelial cells was investigated. When human umbilical endothelial cells were cultured with AGE-bovine serum albumin, viable cell number as well as DNA synthesis was significantly stimulated, whereas prostacyclin production by the endothelial cells was decreased. Antisense oligodeoxyribonucleotides against mRNA coding for AGE receptor were found to reverse both the AGE-induced growth stimulation and the inhibition of prostacyclin production in endothelial cells. These results thus suggest that AGE ligand-receptor interactions in endothelial cells can promote angiogenesis and thrombogenesis, leading to the development of diabetic vascular complications.
Yamagishi
S
S
Department of Biochemistry, Kanazawa University School of Medicine, Japan.
Yamamoto
Y
Y
Harada
S
S
Hsu
C C
CC
Yamamoto
H
H
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D000067759:Receptor for Advanced Glycation End Products; D044006:Receptors, Epoprostenol; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000502:physiology*; D012710:Serum Albumin, Bovine; D013873:Thionucleotides; D013997:Time Factors; D014471:Umbilical Veins
D017127:Glycation End Products, Advanced; D016376:Oligonucleotides, Antisense; D000067759:Receptor for Advanced Glycation End Products; D044006:Receptors, Epoprostenol; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D013873:Thionucleotides; D012710:Serum Albumin, Bovine; D015121:6-Ketoprostaglandin F1 alpha; D004247:DNA; D011464:Epoprostenol