Cloning of the human sodium lodide symporter.
The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.
Smanik
P A
PA
Department of Physiology, Ohio State University, Columbus 43210, USA.
Liu
Q
Q
Furminger
T L
TL
Ryu
K
K
Xing
S
S
Mazzaferri
E L
EL
Jhiang
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007454:Iodides / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010472:Perchlorates / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D007454:Iodides; D008565:Membrane Proteins; D010472:Perchlorates; D027981:Symporters; D000099209:Sodium-Iodide Symporters