title: Structural characterization of red wine rhamnogalacturonan II.
abstract: The pectic polysaccharide rhamnogalacturonan II (RG-II), which accounts for approximately 20% of the ethanol-precipitable polysaccharides in red wine, has been isolated from wine polysaccharides by anion-exchange chromatography. Four fractions enriched with RG-II were obtained and the RG-II then purified to homogeneity by Concanavalin A affinity and size-exclusion chromatographies. The glycosyl-residue compositions of the four RG-IIs are similar; all the RG-IIs contain the monosaccharides (apiose, 2-O-methyl-L-fucose, 2-O-methyl-D-xylose, Kdo, Dha, and aceric acid) that are diagnostic of RG-II. The glycosyl-linkages of the neutral and acidic sugars, including aceric acid, were determined simultaneously by GC-EIMS analysis of the methylated alditol acetates generated from per-O-methylated and carboxyl-reduced RG-II. Two of the RG-IIs contain boron, most likely as a borate di-ester that cross-links two molecules of RG-II together to form a dimer. The dimer contains 3'- and 2,3,3'-linked apiosyl residues whereas the monomer contains only 3'-linked apiosyl residues which suggests that the borate di-ester is located on at least one of the apiosyl residues of RG-II. Although the wine RG-IIs all have similar structures they are not identical since they differ in the length and degree of methyl-esterification of the RG-II backbone and in the presence or absence of borate di-esters. Nevertheless, these studies show that the major structural features of wine and primary cell wall RG-II are conserved.
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire des Polymères et des Techniques Physico-Chimiques, Montpellier, France.
authors.forename: P
authors.initials: P
authors.lastname: Pellerin
authors.forename: T
authors.initials: T
authors.lastname: Doco
authors.forename: S
authors.initials: S
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Williams
authors.forename: J M
authors.initials: JM
authors.lastname: Brillouet
authors.forename: M A
authors.initials: MA
authors.lastname: O'Neill
mesh_terms: D001881:Borates / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D011096:Polygalacturonase / Q000378:metabolism; D014920:Wine
chemical_list: D001881:Borates; D004952:Esters; D009005:Monosaccharides; D009844:Oligosaccharides; C042492:rhamnogalacturonan II; D010368:Pectins; D011096:Polygalacturonase