[Immunohistochemical localization of acidic and basic fibroblast growth factor through corneal neovascularization in vivo and in vitro].
Neovascularization on the center of rabbit cornea was induced by 5N.NaOH alkali burns. We studied the change in localization of acidic fibroblast growth factor (a-FGF) and basic fibroblast growth factor (b-FGF) through corneal neovascularization with immunohistochemistry, using eyes which we enucleated on the 3rd, 7th, and 14th day. Moreover, by co-cultivation of rabbit corneal stromal cells and adrenal cortical vascular endothelium of bovine, a capillary-like code was induced, in which we also studied the localization of a-FGF and b-FGF on the 3rd, 7th, and 14th day. On the 14th day after the alkali burn we recognized intrastromal neovascularization and positive staining of a-FGF and b-FGF around it. Strong staining of a-FGF was observed in goblet cells through the experimental period. In control eyes we recognized positive reaction of a-FGF in corneal and conjunctival epithelium. In co-cultured cells, we recognized positive staining of both a-FGF and b-FGF around the capillary-like code which was induced among the corneal stromal cells.
Hayashi
N
N
Department of Ophthalmology, National Defense Medical College, Japan.
Nakayasu
K
K
Okisaka
S
S
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016510:Corneal Neovascularization / Q000139:chemically induced / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007150:Immunohistochemistry; D011817:Rabbits; D012972:Sodium Hydroxide
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D012972:Sodium Hydroxide