title: Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.
abstract: The humoral immune responses against three different antigens of Brucella abortus were monitored by enzyme-linked immunosorbent assay in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9. Immunoglobulin G (IgG) and IgM responses against (i) B. abortus lipopolysaccharide (LPS), (ii) total cytoplasmic proteins depleted of LPS (LPS-free CYT), and (iii) B. abortus 18-kDa cytoplasmic protein were measured. Vaccinated animals and Yersinia-infected animals developed high anti-LPS IgM and IgG titers, which overlapped with those obtained with sera from B. abortus 544-infected animals used as positive controls. In contrast, only a slight or negative IgG and IgM response against LPS-free CYT and the 18-kDa protein was detected in vaccinated or Yersinia-infected cattle, although its levels were always significantly lower than those of B. abortus 544-infected animals. These data indicate that cytoplasmic proteins of B. abortus could be useful for the differential diagnosis of bovine brucellosis.
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral, Consejo Nacional de Investigaciones Cientificas y Técnicas de Argentina-Universidad de Buenos Aires (IDEHU, CONICET-UBA), Argentina.
authors.forename: P C
authors.initials: PC
authors.lastname: Baldi
authors.forename: G H
authors.initials: GH
authors.lastname: Giambartolomei
authors.forename: F A
authors.initials: FA
authors.lastname: Goldbaum
authors.forename: L F
authors.initials: LF
authors.lastname: Abdón
authors.forename: C A
authors.initials: CA
authors.lastname: Velikovsky
authors.forename: R
authors.initials: R
authors.lastname: Kittelberger
authors.forename: C A
authors.initials: CA
authors.lastname: Fossati
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D003593:Cytoplasm / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D015009:Yersinia Infections / Q000139:chemically induced* / Q000276:immunology; D015008:Yersinia enterocolitica / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides