title: Mutator-induced mutations of the rf1 nuclear fertility restorer of T-cytoplasm maize alter the accumulation of T-urf13 mitochondrial transcripts.
abstract: Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IA153 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4-kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6- and 0.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.
authors.affiliation: USDA-Agricultural Research Service, Iowa State University, Ames 50011, USA. rpwise@iastate.edu
authors.forename: R P
authors.initials: RP
authors.lastname: Wise
authors.forename: C L
authors.initials: CL
authors.lastname: Dill
authors.forename: P S
authors.initials: PS
authors.lastname: Schnable
mesh_terms: D000483:Alleles; D015342:DNA Probes; D018744:DNA, Plant; D008928:Mitochondria; D009154:Mutation; D009693:Nucleic Acid Hybridization; D003313:Zea mays / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018744:DNA, Plant