The genomic structure of the gene encoding the human transforming growth factor beta type II receptor (TGF-beta RII).
The genomic structure of the human transforming growth factor-beta type II receptor gene (TGF-beta RII) was determined by two PCR-based methods, the "long distance sequencer" method and the "promoter finder" method. Genomic fragments containing exons and adjacent introns were amplified by PCR, and the nucleotide sequences were determined by direct sequencing and subcloning sequencing. The TGF-beta RII protein is encoded by 567 codons in 7 exons. This is the first report about the genomic structure of a gene that belongs to the serine/threonine kinase type II receptor subfamily. Knowledge of the genomic structure of the TGF-beta RII gene will facilitate investigation of the TGF-beta signaling pathway in normal human cells and of the aberrations occurring during carcinogenesis.
Takenoshita
S
S
Laboratory of Human Carcinogenesis, National Institutes of Health, Bethesda, Maryland, 20892-4255, USA.
Hagiwara
K
K
Nagashima
M
M
Gemma
A
A
Bennett
W P
WP
Harris
C C
CC
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018125:Receptors, Transforming Growth Factor beta; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II