Identification of C31 and C32 sterols in Pneumocystis carinii hominis-infected human lungs.
Two sterols in autopsied whole lung specimens obtained from Pneumocystis carinii pneumonia patients were detected by gas-liquid chromatography and their structures were elucidated by mass spectrometry and nuclear magnetic resonance spectrometry. Both were in the lanosterol series; the C31 sterol, with a methyl group at C-24, was identified as euphorbol, and the more abundant C32 sterol, with an ethyl group at C-24, is given the trivial name pneumocysterol.
Kaneshiro
E S
ES
Dept. Biol. Sci., Univ. Cincinnati, OH 45221, USA.
Swonger
M
M
Kreishman
G
G
Brooks
E
E
Jayasimhulu
K
K
Parish
E J
EJ
Beach
D H
DH
D002849:Chromatography, Gas; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000032:analysis; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D011020:Pneumonia, Pneumocystis / Q000378:metabolism* / Q000473:pathology
C102334:pneumocysterol; D007810:Lanosterol