RNA partitioning accompanied by adsorption: high-molecular-mass RNA adsorbed at the interface like a particle.
In a potassium phosphate-poly(ethylene glycol) (PEG) system. RNA partitioning was accompanied by adsorption at the interface, depending on the molecular mass. Low-molecular-mass RNA showed the typical partition behavior of a soluble substance. Conversely, high-molecular-mass RNA was significantly adsorbed at the interface in the potassium phosphate-PEG1500 system. The adsorbed amount was proportional to the added amount, regardless of the phase volume ratio. The partitioning of high-molecular-mass RNA showed an amount-ratio-dependent distribution like that of a particle.
Kimura
K
K
Bioengineerig Department, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Kobayashi
H
H
D000327:Adsorption; D008970:Molecular Weight; D009994:Osmolar Concentration; D010710:Phosphates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012997:Solvents / Q000737:chemistry*
D010710:Phosphates; D012331:RNA, Fungal; D012997:Solvents; D011092:Polyethylene Glycols