Eucalypt alpha-tubulin: cDNA cloning and increased level of transcripts in ectomycorrhizal root system.
Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (No. 270) of the ectomycorrhizal basidiomycete Pisolithus tinctorius caused no changes in root transcript levels of EgTubA1, whereas a drastic up-regulation in its expression was observed between 3 to 4 days after contact with the aggressive isolate 441. This enhanced alpha-tubulin expression coincided with the increase lateral root formation induced by fungal colonisation. The changes in alpha-tubulin expression support a role for cytoskeleton components in ectomycorrhiza development.
Diaz
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Equipe de Microbiologie Forestière, Centre de Recherches de Nancy, Institut National de la Recherche Agronomique, Champenoux, France.
Martin
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Tagu
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D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000472:pathogenicity / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005052:Eucalyptus / Q000235:genetics / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis; D014158:Transcription, Genetic / Q000502:physiology; D014404:Tubulin / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D014404:Tubulin