Effects of cryopreservation on immune responses: IX. Stimulus-mediated dichotomy in IL-10 production by frozen human peripheral blood mononuclear cells.
The effects of freezing on phytohemagglutinin (PHA) and lipopolysaccharide (LPS)-induced interleukin-10 (IL-10) production by human peripheral blood mononuclear cells (PBMC) were studied. The freezing process had a divergent effect on the production of IL-10 by PBMC. Frozen PBMC produced significantly smaller amounts of IL-10 in response to PHA stimulation while secreting significantly larger quantities in response to LPS activation. In vitro irradiated PBMC produced significantly smaller amounts of IL-10 in response to both PHA and LPS stimulation. The results indicate that the functional inactivation of a naturally occurring subset of cryosensitive and radiosensitive immunodownregulatory cells is responsible for the observed divergence. They further suggest that, in addition to other mechanisms, a reduction in the secretion of this cytokine synthesis inhibitory factor by the frozen PHA-activated cells could have contributed to the previously reported, augmented IL-2 and IFN-gamma-secreting capabilities of frozen PBMC. The significance of this freezing-induced deviation in IL-10 secretion by PBMC in relation to cancer therapy is discussed.
Venkataraman
M
M
Department of Medicine, Mount Sinai Hospital Medical Center, Chicago, IL 60608 USA.
D000328:Adult; D001793:Blood Preservation; D015925:Cryopreservation; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D016753:Interleukin-10