Preservation of the latissimus dorsi muscle during cardiomyoplasty surgery.
BACKGROUND
Cardiomyoplasty surgery has been shown to be associated with damage and degeneration of the assisting skeletal muscle. The purpose of this study was to use ischemic (short-term) and thermal (long-term) preconditioning to protect the muscle during surgery and the subsequent ischemia.


METHODS
Three 10-minute cycles of ischemia-reperfusion were accomplished noninvasively on goat latissimus dorsi muscle (LDM) immediately prior to surgery. In another experiment, LDM was noninvasively heat shocked for 20 minutes at 42 degrees C 24 hours prior to surgery. LDM damage was evaluated 5 days postsurgery using enzyme activities (beta-glucuronidase, beta-GLN; citrate synthase), hydroxyproline, morphology, and blood flow.


RESULTS
The lysosomal enzyme, beta-GLN, was significantly increased (43%, p < 0.05) by surgical dissection and remained high in the ischemic preconditioned LDM (58%, p < 0.05) and in the heat shocked LDM (57%, p < 0.05).


CONCLUSIONS
These findings show that these two protective protocols do not reduce the muscle damage that occurs during surgical preparation of the LDM for cardiomyoplasty.
Ianuzzo
C D
CD
Deborah Research Institute, Browns Mills, NJ 08015-1799, USA.
Ianuzzo
S E
SE
Locke
M
M
Brdlik
C
C
Anderson
W A
WA
Gu
J
J
Klabunde
R E
RE
D000818:Animals; D018421:Cardiomyoplasty / Q000379:methods*; D002950:Citrate (si)-Synthase / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D006041:Goats; D006358:Hot Temperature; D006909:Hydroxyproline / Q000032:analysis; D007511:Ischemia / Q000201:enzymology; D019194:Ischemic Preconditioning / Q000379:methods; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000201:enzymology / Q000473:pathology / Q000637:transplantation; D009336:Necrosis; D012039:Regional Blood Flow; D015424:Reperfusion
D002950:Citrate (si)-Synthase; D005966:Glucuronidase; D006909:Hydroxyproline