title: L-selectin (CD62L) blockade does not impair peritoneal neutrophil emigration or subcutaneous host defense to bacteria in rabbits.
abstract: Neutrophil (PMN) recruitment into systemic inflammatory sites in vivo is thought to be initiated by selectin-mediated endothelial adherence. We explored the role of L-selectin (CD62L) in leukocyte emigration following instillation of bacteria into the peritoneum or s.c. skin in rabbits. Pretreatment with blocking mAb against L-selectin (LAM1.3) reduced peritoneal PMN emigration 4 h after i.p. inoculation with 10(10) CFU of Escherichia coli by only 17% compared with animals receiving a nonblocking L-selectin mAb (LAM1.14). Peritoneal PMNs from saline-treated rabbits demonstrated a complete absence of L-selectin, whereas those from LAM1.3-treated animals retained 43% of their baseline L-selectin expression. This suggests that L-selectin shedding is not a requisite event for PMN emigration under these conditions. In rabbits given s.c. inoculations with either Staphylococcus aureus or E coli, pretreatment with mAb LAM1.3 did not significantly impair PMN emigration at 24 h, nor increase the incidence, size, or associated mortality of resulting abscesses at 7 days compared with animals receiving nonblocking mAb LAM1.14. We conclude that: 1) mAb blockade of L-selectin in vivo only modestly affects acute, E. coli-induced peritoneal PMN emigration; and 2) L-selectin blockade does not increase infectious sequelae associated with s.c. bacterial inoculation. These findings of only mildly reduced PMN emigration into the peritoneum and no alteration in s.c. host defense differ from those reported with L-selectin blockade under other, nonbacterial inflammatory conditions, and suggest that redundant selectin-mediated mechanisms (P- and E-selectin) are sufficient for normal PMN emigration in response to bacterial stimulation.
authors.affiliation: Department of Anesthesiology, University of Washington School of Medicine, Seattle 98195, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Sharar
authors.forename: N N
authors.initials: NN
authors.lastname: Chapman
authors.forename: L C
authors.initials: LC
authors.lastname: Flaherty
authors.forename: J M
authors.initials: JM
authors.lastname: Harlan
authors.forename: T F
authors.initials: TF
authors.lastname: Tedder
authors.forename: R K
authors.initials: RK
authors.lastname: Winn
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D001667:Binding, Competitive / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects*; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000401:mortality / Q000517:prevention & control*; D019041:L-Selectin / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects*; D010529:Peritoneal Cavity / Q000166:cytology; D011817:Rabbits; D012867:Skin / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D002630:Chemotactic Factors; D019041:L-Selectin