Interaction of alpha-MSH and substance P with interfaces containing gangliosides.
In the present work we studied the interaction of alpha-MSH and substance P neuropeptides with gangliosides using lipid monolayers, fluorescence spectroscopy, and differential scanning calorimetry techniques. The positively charged weak amphiphilic neuropeptides did not show surface activity in the range of concentrations tested (0.1-0.3 muM), but they were preferentially able to penetrate monolayers formed by acidic lipids, showing the best interaction with the more complex gangliosides. The general order of interaction found for both peptides is GTIh > GDIa = GMI > DLPA > sulphatide. Neither neuropeptide interacted with phosphatidylcholine monolayers above 10 mN.m-1. The binding of alpha-MSH to GMI micelles followed by changes in the fluorescence of its tryptophan residue takes place with an increase in the hydrophobic environment of the neuropeptide. An apparent dissociation constant of 13 muM was estimated for this process. Similar result was found with GMI:DMPC vesicles (1:10 molar ratio). The thermotropic profile of GMI micelles is modified in the presence of the neuropeptides. The calorimetric enthalpy of GMI transition increased 21% and 37% in the presence of alpha-MSH and substance P, respectively. Both neuropeptides induced the same increment in the transition temperature Tm from 19 to 20.5 degrees C. The basic physicochemical studies herein indicated that both positively charged neuropeptides, alpha-MSH and substance P, interact with interfaces containing gangliosides in a mainly electrostatic form, whereas the hydrophobic interaction seems to play a secondary role.
González
M
M
Departamento de Química Biológica-CIQUIBIC, Universidad Nacional de Córdoba, Argentina.
Lezcano
N
N
Celis
M E
ME
Fidelio
G D
GD
D000595:Amino Acid Sequence; D000818:Animals; D002152:Calorimetry, Differential Scanning; D005732:Gangliosides / Q000378:metabolism*; D008823:Micelles; D008969:Molecular Sequence Data; D051381:Rats; D013050:Spectrometry, Fluorescence; D013373:Substance P / Q000378:metabolism*; D000521:alpha-MSH / Q000378:metabolism*
D005732:Gangliosides; D008823:Micelles; D013373:Substance P; D000521:alpha-MSH