Probing the conformation of the human T-lymphotropic virus I envelope protein complex with monoclonal antibodies.
We are investigating the binding of a series of monoclonal antibodies to native and detergent-treated human T-lymphotropic virus I (HTLV-I) envelope proteins to explore their conformation. A comparison of our data with previously published findings suggests that a central neutralization domain (aa 175-200) is folded such that only short stretches are exposed at the surface of the native envelope protein complex. However, the complete domain becomes accessible after treatment with mild non-ionic detergents, suggesting that envelope subunit interaction may partially obscure this domain. We further provide immunochemical evidence that a region containing a heptad repeat in the extracellular part of the transmembrane protein is folded towards the interior of the HTLV-I envelope complex.
Carrington
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Virology Laboratory, Chester Beatty Laboratories, Institute of Cancer Research, London, UK. christine@trinidad.net
Paul
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N
Cordell
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Schulz
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TF
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D018604:Epitope Mapping; D015686:Gene Products, env / Q000737:chemistry* / Q000276:immunology; D015486:HTLV-I Antigens / Q000737:chemistry* / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry* / Q000276:immunology; D018411:Spodoptera / Q000166:cytology; D054299:env Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D015686:Gene Products, env; D015486:HTLV-I Antigens; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C055265:gp21 protein, Human T-lymphotropic virus 1; C055266:gp46 protein, Human T-cell leukemia virus type I