title: Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
abstract: Commercially-available monoclonal antibodies to B lymphocytes were evaluated for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed, paraffin-embedded tissues using an avidin biotin complex immunoperoxidase immunohistochemical technique. Three monoclonal antibodies: F46A and F72A raised to "carnivore" B lymphocytes and RA3.6B2 raised to murine B lymphocytes, stained B lymphocyte-dependent areas of frozen feline lymphoid tissue. In addition, antibody RA3.6B2 stained B lymphocyte dependent areas in formalin-fixed, paraffin-embedded feline tissues. There was no staining of T lymphocyte-dependent areas in either frozen or formalin-fixed tissues. Dual parameter flow cytometry, using an anti-pan-T lymphocyte antibody, revealed that greater than 99% of the cells stained by RA3.6B2 are a population distinct from T lymphocytes. F46A was shown to stain a sub-population of those cells stained with RA3.6B2. These antibodies may be useful in the identification of feline B lymphocytes using immunohistochemistry and flow cytometry and thereby provide additional tools to study B lymphocyte ontogeny and the significance of lymphocyte phenotype in lymphoid neoplasia in cats.
authors.affiliation: Department of Pathology, Western College of Veterinary Medicine, Saskatoon, Saskatchewan.
authors.forename: C E
authors.initials: CE
authors.lastname: Monteith
authors.forename: B J
authors.initials: BJ
authors.lastname: Chelack
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: D M
authors.initials: DM
authors.lastname: Haines
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D002415:Cats; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005404:Fixatives; D005434:Flow Cytometry / Q000662:veterinary; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000379:methods*; D008198:Lymph Nodes / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D016612:Paraffin Embedding / Q000379:methods / Q000662:veterinary*; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D005404:Fixatives; D005557:Formaldehyde