In vitro characterization of a novel avian haemopoietic growth factor derived from stromal cells.
To better define the role of the chicken haemopoietic microenvironment in supporting haemopoiesis, a continuous cell line was generated by RSV transformation of avian spleen stromal cells (SSL-1). Supernatants from this line were found to contain haemopoietic growth factor activity as measured by the ability to induce proliferation and differentiation of precursor cells present in embryonic and post-hatched haemopoietic tissues. Comparison of cultures grown in the presence of cMGF and SSL-1 conditioned media (CM) revealed that both cytokine sources induced similar types of cell populations. Both sources supported the proliferation of predominantly macrophage-like cells based on colony morphology, differential staining, non-specific esterase staining, and phagocytosis activity. Interestingly, SSL-1 does not express any message for cMGF, nor does it secrete any IL-2 or interferon activities suggesting that the growth factor activities seen in SSL-1 are novel.
Siatskas
C
C
Department of Pathology and Immunology, Monash University Medical School, Prahran, Melbourne, Australia.
McWaters
P G
PG
Digby
M
M
Lowenthai
J W
JW
Boyd
R L
RL
D000818:Animals; D030161:Avian Proteins; D001854:Bone Marrow Cells; D002460:Cell Line; D002469:Cell Separation; D002642:Chick Embryo; D002645:Chickens / Q000502:physiology*; D017077:Culture Media, Conditioned; D016207:Cytokines; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000235:genetics / Q000494:pharmacology; D006410:Hematopoiesis / Q000502:physiology*; D016298:Hematopoietic Cell Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells; D017154:Stromal Cells / Q000737:chemistry* / Q000166:cytology; D014018:Tissue Distribution
D030161:Avian Proteins; D017077:Culture Media, Conditioned; D016207:Cytokines; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D007376:Interleukin-2; C059626:MGF protein, chicken; D011994:Recombinant Proteins; D007372:Interferons