title: PeptideYY and insulin coexist in beta-granules in B cells of the Madagascan lizard, Zonosaurus laticaudatus.
abstract: PYY, a 36-amino-acid peptide belonging to the PP family, is often colocalized with glucagon in A cells in the gastroenteropancreatic system of vertebrates. However, both immunocytochemical and immunohistochemical methods reveal this peptide in insulin-containing beta-granules in the lizard Zonosaurus laticaudatus. Absorption tests of PYY antiserum with insulin did not abolish the immunostaining of beta-granules with PYY antiserum, ruling out a cross-reaction between the PYY antiserum and insulin. This feature may be a reflection of the persistence of an ontogenetic character or a result of an adaptative mechanism that selectively activates the PP family of genes in the cellular line of B rather than of A cells.
authors.affiliation: Department of Evolutionary and Comparative Biology, University of Naples "Federico II,", Naples, 80134, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Putti
authors.forename: A
authors.initials: A
authors.lastname: Della Rossa
mesh_terms: D000042:Absorption / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D005768:Gastrointestinal Hormones / Q000032:analysis* / Q000276:immunology; D005934:Glucagon / Q000032:analysis / Q000276:immunology; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods; D007328:Insulin / Q000032:analysis* / Q000276:immunology; D007515:Islets of Langerhans / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D008116:Lizards / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D010191:Pancreatic Polypeptide / Q000032:analysis / Q000276:immunology; D019894:Peptide YY; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits
chemical_list: D005768:Gastrointestinal Hormones; D007106:Immune Sera; D007328:Insulin; D010455:Peptides; D019894:Peptide YY; D010191:Pancreatic Polypeptide; D005934:Glucagon