title: Testicular cell cytoskeleton in the newt, Triturus marmoratus marmoratus, during the annual cycle.
abstract: Light and electron microscopy immunohistochemical studies and Western blotting analysis of cytoskeletal proteins have been carried out in the testis of the marbled newt (Triturus marmoratus marmoratus) during the annual testicular cycle. The present findings revealed homologies and differences with regard to those reported in the testes of mammals and other vertebrates. Changes in immunohistochemical expression have also been detected in the course of the annual cycle. Actin and tubulin, which were scanty and diffusely located in spermatogonia and spermatocytes, increased their expression and reorganized during spermiogenesis. Vimentin and keratin, undetected in spermatogonia and spermatocytes, were expressed in differentiating spermatids and spermatozoa. In these cells, actin might be related with the connection of the axial fiber to the undulating membrane and the coordination of movement by both structures, while vimentin might be involved in the maintenance of the spatial relationship between the axoneme and the marginal fiber. During the first stages of spermatogenesis, the cytoplasm of Sertoli cells (follicular cells) showed a diffuse immunoreaction to actin, myosin, and tubulin and no vimentin immunolabeling. In advanced spermiogenesis, the follicular cells showed an intense immunoreaction to actin, myosin, tubulin, and vimentin in the apical projections that surrounded the spermatid heads. These apical cytoskeletal components might be involved in spermatid elongation, since the spermatids display no manchette, and in spermatozoon positioning and grouping. The colocalization of myosin and actin in the follicular cells suggests that actin filaments from contractile bundles and that contraction might be involved in changes in the Sertoli cell shape that accompany germ cell development during spermatogenesis. The interstitial cells immunostained to actin, myosin, tubulin, and vimentin. These cells, together with follicular cells, seemed to form the glandular tissue cells which showed a similar immunophenotype. The cells that surrounded the efferent duct epithelium immunostained to desmin, and they are probably contractile cells involved in sperm evacuation.
authors.affiliation: Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Ramos
authors.forename: M P
authors.initials: MP
authors.lastname: De Miguel
authors.forename: M I
authors.initials: MI
authors.lastname: Arenas
authors.forename: B
authors.initials: B
authors.lastname: Fraile
authors.forename: R
authors.initials: R
authors.lastname: Paniagua
mesh_terms: D000204:Activity Cycles; D000818:Animals; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D013737:Testis / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D014319:Triturus / Q000033:anatomy & histology*
chemical_list: D003598:Cytoskeletal Proteins