title: Evaluation of an automated colorimetric assay for the measurement of lipase activity in canine sera.
abstract: An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were < 6.8% and < 8.3%, respectively. Linearity was determined to be at least 1366 U/L. Canine serum lipase concentrations attained using the colorimetric method were compared to both titrimetric and dry-film methods for measuring serum lipase activity, resulting in significant (P < or = 0.05) correlation coefficients of 0.92 and 0.77, respectively. Canine serum lipase concentrations measured using the colorimetric assay on 2 different automated analyzers had a significant (P < or = 0.05) correlation coefficient of 0.92. A laboratory reference range using serum samples from 56 healthy dogs (0-561 U/L) was established. There were no significant (P < or = 0.05) differences in mean serum lipase concentrations comparing male and female dogs or comparing young dogs (< or = 3 y) to mature (4-7 y) and older (> 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum lipase activity and offered several advantages, including small sample volume and short analysis time.
authors.affiliation: Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown.
authors.forename: A L
authors.initials: AL
authors.lastname: Mackenzie
authors.forename: S A
authors.initials: SA
authors.lastname: Burton
authors.forename: D W
authors.initials: DW
authors.lastname: Olexson
authors.forename: B S
authors.initials: BS
authors.lastname: Horney
authors.forename: D J
authors.initials: DJ
authors.lastname: Honor
authors.forename: P B
authors.initials: PB
authors.lastname: Maloney
authors.forename: S D
authors.initials: SD
authors.lastname: Buczkowski
mesh_terms: D000818:Animals; D003124:Colorimetry / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D005260:Female; D016014:Linear Models; D008049:Lipase / Q000097:blood*; D008297:Male; D013997:Time Factors
chemical_list: D008049:Lipase