title: The effect of the crp genotypes on the transformation efficiency in Escherichia coli.
abstract: We have found that a significant difference exists in transformation efficiency between the crp+/crp- isogenic pair of strains of Escherichia coli, with the efficiency being much higher in crp- than in crp+. The ratio of transformation efficiency between crp+ and crp- strains depends very little on the plasmid size. This observation suggests that the difference of the transformation efficiency is due to mechanisms other than a crp-regulated endonuclease. The crp gene is one of the first specific genes that have been shown to affect transformation efficiency.
authors.affiliation: Department of Public Health, Kansai Medical University, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Umemoto
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: N
authors.initials: N
authors.lastname: Nakazono
authors.forename: Y
authors.initials: Y
authors.lastname: Sugino
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000235:genetics; D010957:Plasmids; D014162:Transfection; D014169:Transformation, Bacterial; D014170:Transformation, Genetic
chemical_list: D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein