Immune cytotoxic activity of human eosinophils against Trichinella spiralis newborn larvae.
The ability of human eosinophils to kill the newborn larvae (NBL) of Trichinella spiralis of different maturation status, in the presence of antibody, was studied. A cytotoxic in vitro test was performed using NBL less than 2 h of age (NBL2) or NBL maintained in culture at 37 degrees C for 20 h (NBL20), peripheral blood eosinophils, anti-Trichinella serum and human fresh serum as source of complement. Under these experimental conditions eosinophils from normal individuals attached to NBL2 as well as to NBL20 but only the latter were killed. On the other hand, eosinophils from volunteers with eosinophilia killed NBL regardless of larval age. Neither adherence nor significant mortality was observed in the absence of immune serum. These results indicate that NBL maturation and eosinophil activation status are crucial for antibody-dependent cellular cytotoxic reaction (ADCC).
Venturiello
S M
SM
Humoral Immunity Studies Institute-CONICET, Department of Microbiology, Immunology & Biotechnology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Giambartolomei
G H
GH
Costantino
S N
SN
D000818:Animals; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D007814:Larva; D051381:Rats; D017208:Rats, Wistar; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood