Molecular cloning of cDNA for rat brain GD3-synthase.
The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.
Zeng
G
G
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614, USA.
Gao
L
L
Ariga
T
T
Yu
R K
RK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012799:Sialyltransferases / Q000235:genetics*
D018076:DNA, Complementary; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase