Comparison of hepatotoxicity of 1,2-, 1,3- and 1,4-dibromobenzenes: the dynamics of changes of selected parameters of liver necrosis in acute poisoning in mice.
Various doses of dibromobenzene isomers (1,2-dBB, 1,3-dBB, 1,4-dBB) were administered (i.p.) to BALB mice. The levels of reduced glutathione (GSH) and malondialdehyde (MDA) in the liver, and glutamate-pyruvate transaminase (GPT) (EC.2.6.1.2) gamma-glutamyltransferase (gamma-GT) (EC.2.3.2.2) and triglycerides (TG) in the serum were estimated. A considerable decrease of GSH was observed between 2 and 12 h after administration of the compounds. Increases in serum GPT activity (up to 100-fold) and gamma-GT (three-to fivefold) were observed after treatment using 1,2- and 1,3-dibromobenzenes; TG decreased in concentration initially and then slightly increased. Histopathological examination confirmed the strong necrotic effect of 1,2- and 1,3-dBB isomers. No such changes (elevation of serum GPT activity and necrosis) were noticed after 1,4-dBB.
Szymańska
J A
JA
Department of Toxicological Chemistry, Medical University, Lódź, Poland.
Bruchajzer
E
E
Sporny
S
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001969:Bromobenzenes / Q000506:poisoning / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009336:Necrosis; D051381:Rats; D014280:Triglycerides / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
D001969:Bromobenzenes; D014280:Triglycerides; C102880:1,3-dibromobenzene; D008315:Malondialdehyde; C102879:1,2-dibromobenzene; C066656:1,4-dibromobenzene; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D005978:Glutathione