Effect of acute exhausting exercise on cytokine gene expression in men.
Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBLs using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using in vitro testing, direct gene expression of TNF-alpha decreased after the stress test (p < 0.008). However, the 47% decrease was relatively small and of questionable biological significance. Levels of IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, and INF-gamma did not change.
Natelson
B H
BH
Chronic Fatigue Syndrome Center, UMDNJ-New Jersey Medical School, Newark 07013, USA.
Zhou
X
X
Ottenweller
J E
JE
Bergen
M T
MT
Sisto
S A
SA
Drastal
S
S
Tapp
W N
WN
Gause
W L
WL
D000328:Adult; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D015444:Exercise / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D016133:Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha