Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined.
Norman
H A
HA
Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
Mischke
C F
CF
Allen
B
B
Vincent
J S
JS
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification*; D008049:Lipase; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification*; D012233:Rhizopus; D018724:Spinacia oleracea / Q000737:chemistry*
D006017:Glycolipids; D010743:Phospholipids; C013694:sulfoquinovosyl diglyceride; D008049:Lipase