Variable immunoreactivity of D-dimer preparations for monoclonal antibody DD-3B6/22.
The monoclonal antibody DD-3B6/22, which is specific for crosslinked fibrin breakdown products, is used clinically in the diagnosis and monitoring of certain thrombotic conditions and has potential as a delivery agent for in vivo clot localisation. Laboratory preparations of D-dimer are used as reference standards in DD-3B6/22 assay systems. In this study, four D-dimer preparations, produced using a standardised method, were presented to the monoclonal antibody, DD-3B6/22 in different antigenic formats and immunoreactivity was assessed using a variety of methods. The results show a variability in reactivity of the D-dimer samples to DD-3B6/22; one preparation (QUT2) was immunoreactive only when immobilised on a surface and not when in solution. The implications of variable immunorecognition of target antigens are discussed with regard to diagnostic standards and in vivo clot localisation.
Wylie
F G
FG
Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.
Walsh
T P
TP
D000911:Antibodies, Monoclonal / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005338:Fibrin Fibrinogen Degradation Products / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D012996:Solutions
D000911:Antibodies, Monoclonal; D005338:Fibrin Fibrinogen Degradation Products; D012996:Solutions; C036309:fibrin fragment D