title: Allergenic cross-reactivities in flea-reactive canine serum samples.
abstract: OBJECTIVE
  To determine whether flea extract could be determined (via ELISA) to share allergenic epitopes with other insects, and to determine whether sera with different reactivities to insect extracts have different cross-reactivity patterns.
  
  
  METHODS
  69 canine serum samples that were selected from samples submitted for routing ELISA allergy testing and had previously been found to have high reactivities to flea.
  
  
  METHODS
  Each serum sample was assessed by means of a direct ELISA for IgE binding to 11 common insects. Samples that were reactive primarily to flea extract alone were designated pool 1, samples that were reactive to small numbers of insects were designated pool 2, and samples that were reactive to all or almost all insects were designated pool 3. Samples that did not have any apparent patterns of cross-reactivity were not included in the rest of the study. Inhibition ELISA techniques were used with the 3 serum pools to determine whether multiple insect extracts inhibited reactivity on flea-coated ELISA plates. Those extracts were used to coat ELISA plates, and reciprocal inhibition studies were then performed.
  
  
  RESULTS
  Black fly, black ant, and cockroach extracts were capable of > 50% inhibition of flea solid-phase IgE binding with all 3 serum pools. In the ELISA inhibition studies, flea extract was able to inhibit IgE binding to each extract with all pools, confirming reciprocal inhibition.
  
  
  CONCLUSIONS
  Inhibition of IgE binding to solid-phase flea antigen by black ant, black fly, and cockroach extracts suggested sharing of allergenic epitopes among these species. Reciprocal inhibition studies further confirmed these findings. These results indicated in vitro cross-reactivity between flea, black ant, black fly, and cockroach extracts. These results need to be further investigated in vivo.
  
  
  CONCLUSIONS
  It is possible that dogs may become sensitized to fleas via exposure to other insects, and flea allergenic dogs may have signs of pruritus, in the absence of fleas, if exposed to cross-reactive insects.
authors.affiliation: Department of Companion Animals and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Pucheu-Haston
authors.forename: T J
authors.initials: TJ
authors.lastname: Grier
authors.forename: R E
authors.initials: RE
authors.lastname: Esch
authors.forename: D E
authors.initials: DE
authors.lastname: Bevier
mesh_terms: D000818:Animals; D001000:Ants / Q000276:immunology; D003058:Cockroaches / Q000276:immunology; D003429:Cross Reactions; D004175:Diptera / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D007073:Immunoglobulin E / Q000097:blood*; D007313:Insecta / Q000276:immunology*; D005423:Siphonaptera / Q000276:immunology*
chemical_list: D000939:Epitopes; D007073:Immunoglobulin E