title: Flow cytometrical comparison of different semi-automatic ways of measuring S-phase fractions using fresh and formalin-treated normal endometrial tissue.
abstract: Histopathologically normal endometrial tissue from 250 patients was prepared for DNA measurements according to the technique of Schütte, combined with the techniques of Vindelöv and Thorntwaite for formalin-fixed material; the latter methods were used alone for fresh material. To estimate the influence of different analysis methods we used two semi-automatic methods (Cellfit, MacCycle) and a manual method. Differencies between these methods were found regarding CV (the width of the peak), and S-phase fractions [S%, (S + G2)%]. We therefore conclude that histopathologically normal tissue must first be evaluated by these methods to establish the range of normal values for a certain organ/tissue, before a certain set of values are applied in the clinical situation.
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Lindahl
authors.forename: R
authors.initials: R
authors.lastname: Willén
mesh_terms: D001331:Automation; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005404:Fixatives; D005434:Flow Cytometry; D005557:Formaldehyde; D006801:Humans; D012016:Reference Values; D016196:S Phase
chemical_list: D005404:Fixatives; D005557:Formaldehyde