An RBM homologue maps to the mouse Y chromosome and is expressed in germ cells.
We have isolated a murine homologue of the human Y-linked RBM genes (previously termed YRRM), a gene family implicated in spermatogenesis and which encodes proteins containing an RNA recognition motif. A number of very similar copies of this gene (called Rbm) are present in the mouse. These mouse homologues are also Y-encoded, mapping on the short arm of the chromosome, proximal to Sry. Expression is confined to the testis, specifically the germ line on the basis of lack of expression in the germ-line negative testes of adult sex-reversed mice. The timing of Rbm transcription is regulated, with fetal message levels reaching a peak at 15 d.p.c. Transcripts are clearly detectable by 4 days after birth and reach their highest level at 14 d.p.p. which is the time at which the Y chromosome condenses during meiotic prophase. These results suggest that Rbm is functionally involved in germline RNA metabolism.
Elliott
D J
DJ
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Ma
K
K
Kerr
S M
SM
Thakrar
R
R
Speed
R
R
Chandley
A C
AC
Cooke
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D012734:Disorders of Sex Development; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D051876:Sex-Determining Region Y Protein; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology; D013737:Testis / Q000737:chemistry; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C501499:RBMY1A1 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C501500:Rbmy protein, mouse; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors