title: The prognostic value of NCAM, p53 and cyclin D1 in resected non-small cell lung cancer.
abstract: Expression of the neural cell adhesion molecule, NCAM, in frozen sections has been associated with decreased postoperative survival in non-small cell lung carcinoma. Of the various isoforms of NCAM described, the highly sialylated isoform plays a role in the migration of embryonal cells from the neural crest and is expressed by highly malignant tumours such as small cell lung carcinomas. We investigated the clinical significance of expression of this NCAM isoform as a prognostic factor in a series of 96 non-small cell lung carcinomas resected with curative intent. We also evaluated the effect of microwave pre-treatment of formalin-fixed, paraffin-embedded sections on the NCAM immunostaining and related the outcome to the postoperative clinical course of disease. In addition, in an attempt to extend our search for possible molecular markers of unfavourable prognosis in lung cancer, we evaluated increased immunostaining for p53 and cyclin D1 in the same series. We did not find a significant relation between expression of NCAM or its highly sialylated isoform and the length of postoperative survival. The numbers of positive cases (9 and 14, respectively) were relatively low. Increased p53 and cyclin D1 immunostaining (50 and 55 of the 96 tumours) failed to show a significant relation with postoperative survival. In our material, tumour stage was the only significant prognostic factor.
authors.affiliation: Department of Pulmonology C-3-P, University Hospital Leiden, The Netherlands.
authors.forename: H B
authors.initials: HB
authors.lastname: Kwa
authors.forename: R J
authors.initials: RJ
authors.lastname: Michalides
authors.forename: J H
authors.initials: JH
authors.lastname: Dijkman
authors.forename: W J
authors.initials: WJ
authors.lastname: Mooi
mesh_terms: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D019938:Cyclin D1; D016213:Cyclins / Q000032:analysis*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D019006:Neural Cell Adhesion Molecules / Q000032:analysis*; D015513:Oncogene Proteins / Q000032:analysis*; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D011379:Prognosis; D016707:Tissue Fixation; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D016213:Cyclins; D019006:Neural Cell Adhesion Molecules; D015513:Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D005557:Formaldehyde