Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1).
We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV-approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI-1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.
Brzoska
P M
PM
Department of Radiation Oncology, University of California, San Francisco, California, 94143, USA.
Chen
H
H
Levin
N A
NA
Kuo
W L
WL
Collins
C
C
Fu
K K
KK
Gray
J W
JW
Christman
M F
MF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D003599:Cytoskeleton / Q000737:chemistry; D004791:Enzyme Inhibitors; D005347:Fibroblasts; D005796:Genes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017386:Sequence Homology, Amino Acid
D004791:Enzyme Inhibitors; C063306:HINT1 protein, human; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D011493:Protein Kinase C