title: Amelioration of hepatocellular integrity and inhibition of sinusoidal oxidative stress by N-acetylcysteine pretreatment in cold ischemia-reperfusion injury of rat liver.
abstract: Further improvements of donor liver preservation are still required in liver transplantation. In the present study, we investigated whether intraportal injection of N-acetylcysteine (NAC) 15 min before flush-out of UW solution (NAC pretreatment) improves liver dysfunction after cold preservation or has a protective effect on sinusoidal oxidative stress. The effect of NAC pretreatment was examined using an isolated perfused rat liver model. The NAC pretreatment significantly reduced sinusoidal oxidative stress relative to a control dextrose 5% injection. Under a glutathione-depleted condition produced by L-buthionine-[S-R]-sulfoximine, the NAC pretreatment also significantly reduced hepatocellular as well as sinusoidal oxidative stress, resulting in improvement of hepatocelllar integrity relative to a control dextrose 5% injection.
authors.affiliation: Laboratoire de Chirurgie Expérimentale de la Fondation Transplantation, Université Louis-Pasteur, Hôpital Universitaire de Hautepierre, Strasbourg, France.
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Boudjema
authors.forename: D
authors.initials: D
authors.lastname: Jaeck
authors.forename: E
authors.initials: E
authors.lastname: Alexandre
authors.forename: P
authors.initials: P
authors.lastname: Imbs
authors.forename: M P
authors.initials: MP
authors.lastname: Chenard
authors.forename: H
authors.initials: H
authors.lastname: Nagasaki
authors.forename: K
authors.initials: K
authors.lastname: Kumada
authors.forename: P
authors.initials: P
authors.lastname: Wolf
authors.forename: J
authors.initials: J
authors.lastname: Cinqualbre
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D019328:Buthionine Sulfoximine; D005978:Glutathione; D000111:Acetylcysteine