title: The staphylococcal insertion sequence IS257 is active.
abstract: The plasmid pJ3356 confers high-level mupirocin resistance on a strain of Staphylococcus aureus isolated from a hospital. The plasmid also carries two copies of IS257. Recombination of an IS257-containing plasmid conferring erythromycin resistance, pOX7-IS, into either of the IS257s of pJ3356 has been observed. The co-integration of pJ3356 and a small plasmid, pOX7, is also reported and involves duplication of one of the IS257s from pJ3356 together with 8 bp of pOX7 at the site of integration. Thus IS257 has been shown to be an active mobile genetic element.
authors.affiliation: Department of Biochemistry, University of Oxford, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Needham
authors.forename: W C
authors.initials: WC
authors.lastname: Noble
authors.forename: K G
authors.initials: KG
authors.lastname: Dyke
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial