Effects of the low-molecular-weight GTP-binding protein RhoA on calcium waves in xenopus oocytes.
In normal Xenopus oocytes, injection of high concentrations of inositol-1,4,5-trisposphate (IP3) always elicited a Ca2+ wave, which propagated from the injection site toward the opposite side of the oocyte. In oocytes injected with C3 toxin, a specific blocker of RhoA, a similar Ca2+ wave was still observed, but propagation of the wave appeared to be retarded compared with normal controls. This apparent retardation seemed to be due to a slower decrease of normally increased Ca2+ rather than to a slower increase of Ca2+. It is suggested that RhoA may play a role in Ca2+ reuptake mechanisms.
Kato
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Department of Integrative Brain Science, Faculty of Medicine, Kyoto University, Japan.
D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D008970:Molecular Weight; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction; D014982:Xenopus laevis; D020742:rhoA GTP-Binding Protein
D015544:Inositol 1,4,5-Trisphosphate; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D002118:Calcium