Impaired defense of intestinal mucosa in mice lacking intestinal trefoil factor.
The mechanisms that maintain the epithelial integrity of the gastrointestinal tract remain largely undefined. The gene encoding intestinal trefoil factor (ITF), a protein secreted throughout the small intestine and colon, was rendered nonfunctional in mice by targeted disruption. Mice lacking ITF had impaired mucosal healing and died from extensive colitis after oral administration of dextran sulfate sodium, an agent that causes mild epithelial injury in wild-type mice. ITF-deficient mice manifested poor epithelial regeneration after injury. These findings reveal a central role for ITF in the maintenance and repair of the intestinal mucosa.
Mashimo
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H
Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Jackson 7, Massachusetts General Hospital, Fruit Street, Boston, MA 02114, USA.
Wu
D C
DC
Podolsky
D K
DK
Fishman
M C
MC
D000818:Animals; D001483:Base Sequence; D002465:Cell Movement; D003092:Colitis / Q000209:etiology / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D016264:Dextran Sulfate / Q000494:pharmacology; D018390:Gene Targeting; D006133:Growth Substances / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D011994:Recombinant Proteins; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D016264:Dextran Sulfate