Development of a radioimmunoassay for SR 31747A, a new sigma ligand, in human plasma.
A specific and sensitive radioimmunoassay was developed for SR 31747A, a new sigma ligand, using a monoclonal antibody. This antibody was produced from spleen lymphocytes of a mouse immunized with SR 31747A coupled to bovine serum albumin via a peptide bond using SR 120684A, a succinamic acid derivative of SR 31747A. Negligible binding occurred when metabolites, obtained by chemical synthesis or by "in-vitro" incubation with hepatic microsomal fraction, were tested for cross-reactivity. A quantitative recovery in serum of the exogenous analyte was obtained for all the concentrations tested and the quantification limit was found to be 0.25 ng ml-1 of SR 31747 (the non-salified derivative). Intra- and inter-assay relative standard deviations ranged from 6.3-10.9 and from 5.3% to 15.4% respectively. Furthermore, comparison of results from samples which were assayed by radioimmunoassay and gas chromatography demonstrated an excellent correlation (r = 0.984).
Dussossoy
D
D
Sanofi Recherche, Immunology Department, Montpellier, France.
Belugou
S
S
Pastor
G
G
Combalbert
J
J
Casellas
P
P
D000814:Aniline Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002849:Chromatography, Gas; D003429:Cross Reactions; D003510:Cyclohexanes / Q000097:blood*; D006801:Humans; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D011863:Radioimmunoassay; D017480:Receptors, sigma / Q000097:blood*; D015203:Reproducibility of Results; D013154:Spleen / Q000166:cytology; D013386:Succinates / Q000008:administration & dosage / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000814:Aniline Compounds; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000911:Antibodies, Monoclonal; D003510:Cyclohexanes; D017480:Receptors, sigma; C102690:SR 120684A; D013386:Succinates; C088447:SR 31747