title: Expression of intercellular and vascular cell adhesion molecules and class II major histocompatibility antigens in human lungs: lack of influence by conditions of organ preservation.
abstract: BACKGROUND
  The expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and class II major histocompatibility complex antigens was studied in control lung tissue and preserved human donor lungs. The three controls were represented by wedge biopsy specimens taken from non-neoplastic lung surrounding bronchogenic carcinomas.
  
  
  METHODS
  Nine lungs were harvested from six brain-dead donors, flushed with Euro-Collins solution or low potassium-dextran-glucose solution, and stored at 1 degree C or 10 degrees C. Samples of the latter organs were taken at the time of surgical harvest (baseline) and after 2, 12, 24, and 48 hours of preservation time. Immunostains with monoclonal antibodies against intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and class II major histocompatibility complex molecules were performed on all samples, and the relative presence of these determinants was evaluated.
  
  
  RESULTS
  In both the controls and preserved lungs, intercellular adhesion molecule-1 expression was intense in the septal capillary endothelium and alveolar pneumocytes, but essentially absent in bronchial epithelium. Vascular cell adhesion molecule-1 was moderately to strongly labeled in the endothelia of large and small blood vessels of all types, and it was not seen in other cell types. Class II major histocompatibility complex antigens were variably observed in pulmonary epithelial cells, but they were not expressed by endothelia. There appeared to be no significant difference in the immunohistologic density of intercellular adhesion molecule-1 or vascular cell adhesion molecule-1 immunostaining in allografts at the specified time points of preservation; this conclusion was confirmed by Western blot analysis. Similar findings pertained to staining results for human leukocyte DR antigens. There was likewise no significant difference in the expression of the three analytes when donor lungs perfused with Euro-Collins solution versus low potassium-dextran-glucose solution were compared; this was also true of organs preserved at 1 degree C versus 10 degrees C.
  
  
  CONCLUSIONS
  These results suggest that, in the immediate post-harvest period, modulations in the expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or class II major histocompatibility complex antigens in pulmonary allografts are not attributable to the influences of preservation conditions.
authors.affiliation: Division of Cardiothoracic Surgery, Washington University Medical Center, St. Louis, Mo 63110, USA.
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: J H
authors.initials: JH
authors.lastname: Ritter
authors.forename: A
authors.initials: A
authors.lastname: Patterson
authors.forename: D M
authors.initials: DM
authors.lastname: Ockner
authors.forename: H
authors.initials: H
authors.lastname: Sawa
authors.forename: T
authors.initials: T
authors.lastname: Mohanakumar
authors.forename: J D
authors.initials: JD
authors.lastname: Cooper
authors.forename: M R
authors.initials: MR
authors.lastname: Wick
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008168:Lung / Q000276:immunology*; D016040:Lung Transplantation / Q000276:immunology*; D008297:Male; D009926:Organ Preservation; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*
chemical_list: D000949:Histocompatibility Antigens Class II; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1