title: Comparison of the properties of concanavalin A and anti-alpha-D-glucose antibodies.
abstract: Concanavalin A and anti-alpha-D-glucose antibodies form precipitin complexes with antigens having alpha-D-glucose as terminal units. The sedimentation rates, molecular weights, gel electrophoretic mobilities, isoelectric points, and immunoglobulin type of Con A and alpha-Ab have been determined. The interactions of the compounds with antigens in the presence of potential inhibitors have been compared. The data show that the interaction of Con A with glucose units occurs with hydrogen bonding at hydroxyl groups at C1,3,4, and 6 and van der Waals bonding at the pyranose ring oxygen. In the alpha-Ab complex with glucose units, in addition to the above bond types, a hydrogen bond at the hydroxyl at C2 occurs and this bond is essential for interaction.
authors.affiliation: Department of Biochemistry and Molecular Biology, Paul M. Althouse Laboratory, Pennsylvania State University, University Park 16802, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Pazur
authors.forename: R E
authors.initials: RE
authors.lastname: Scrano
authors.forename: M D
authors.initials: MD
authors.lastname: Perloff
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000031:analogs & derivatives / Q000276:immunology* / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006003:Glycogen / Q000378:metabolism; D006860:Hydrogen Bonding; D005779:Immunodiffusion; D010504:Periodic Acid / Q000378:metabolism / Q000494:pharmacology; D012709:Serum Albumin / Q000276:immunology / Q000378:metabolism; D000093423:Glycated Serum Albumin
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D010504:Periodic Acid; D003208:Concanavalin A; D006003:Glycogen; C009288:metaperiodate; D005947:Glucose; D000093423:Glycated Serum Albumin