title: Sequential analysis of early hematopoietic reconstitution following allogeneic bone marrow transplantation with fluorescence in situ hybridization (FISH).
abstract: Using in situ hybridization with an X and Y chromosome probe mixture, we have sequentially studied peripheral blood samples from 10 patients (four males/six females) in an HLA-matched allogeneic setting in order to monitor the kinetics of early hematopoietic reconstitution. Interphase cells from smears consisting of purified granulocytic and lymphocytic populations respectively were studied in three patients at 24, 48, 72 and 96 h post-transplant. This period was arbitrarily defined as the immediate post-transplant period. These three patients plus seven others were studied sequentially at days 5, 10, 15, 20, 25 and 50 post-transplant, defined as the intermediate post-transplant period. The X and Y probes were indirectly labelled with rhodamine and fluoresceine isothiocyanate, respectively. Donor neutrophils were detected as early as 24 h post marrow infusion followed by a significant expansion at 48 h. At 96 h post-transplant, the median percentage of donor neutrophils was > 90%. In the immediate post-transplant period, most of the lymphocytes were of recipient origin. However, we have documented a significant expansion in donor lymphocytes, starting at day 5 post-transplant in most patients. Almost complete chimerism for the myeloid and lymphoid lineages was established at days 10 and 25 post-transplant, respectively. All patients engrafted normally according to standard clinical criteria. Follow-up data for those surviving > or = 100 days (eight patients), showed persistence of this pattern of hematopoietic reconstitution in all but one patient. Molecular monitoring of early engraftment has enabled us to unravel a distinct biphasic pattern of myeloid and lymphoid engraftment.
authors.affiliation: Laboratory of Cytogenetics, Maisonneuve-Rosemont Hospital, Montreal, Quebec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Lapointe
authors.forename: L
authors.initials: L
authors.lastname: Forest
authors.forename: P
authors.initials: P
authors.lastname: Lussier
authors.forename: L
authors.initials: L
authors.lastname: Busque
authors.forename: F
authors.initials: F
authors.lastname: Lagace
authors.forename: C
authors.initials: C
authors.lastname: Perreault
authors.forename: D C
authors.initials: DC
authors.lastname: Roy
authors.forename: M
authors.initials: M
authors.lastname: Gyger
mesh_terms: D016026:Bone Marrow Transplantation; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D014184:Transplantation, Homologous