Promotion of synthetic amyloid beta-peptide fibrillization by cell culture media and cessation of fibrillization by serum.
Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid beta 1-40 and beta 1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat-inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid beta fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.
Wegiel
J
J
New York State Institute for Basic Research in Developmental Disabilities, Department of Pathological Neurobiology, Staten Island 10314, USA.
Chauhan
A
A
Wisniewski
H M
HM
Nowakowski
J
J
Wang
K C
KC
Le Vine
H
H
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D006801:Humans; D013997:Time Factors
D016564:Amyloid beta-Protein Precursor; D001798:Blood Proteins; D003470:Culture Media