Concentration-dependent tubular secretion of acetazolamide and its inhibition by salicylic acid in the isolated perfused rat kidney.
An isolated perfused rat kidney (IPK) technique was used to study the effect of salicylic acid (SA) on the excretion of acetazolamide (AZ). Initial experiments were conducted in the absence of interactants at three nominal AZ concentrations (50, 100, and 250 micrograms/ml). Over the concentration range studied, AZ demonstrated net tubular secretion in the IPK. Significant decreases in excretion ratio (4.97 +/- 0.79-2.66 +/- 1.1) and secretory clearance (0.809 +/- 0.23-0.541 +/- 0.28) were observed with increasing AZ concentration, consistent with saturation of tubular secretion. Using a facilitated model for renal secretion, values of tubular transport parameters were obtained from a plot of excretion ratio vs. unbound AZ concentration: tmax = 118 +/- 29.4 micrograms/min, KM = 53.4 +/- 22.4 micrograms/ml, and tmax(A) = 6.31 +/- 2.82 micrograms/min. In the presence of SA (200 micrograms/ml), renal secretion of AZ was inhibited, as demonstrated by significant decreases in renal clearance (0.731 +/- 0.21-0.147 +/- 0.03) and excretion ratio (3.77 +/- 0.82-0.378 +/- 0.07). Although these findings were indicative of a reabsorption component to AZ excretion in the IPK that had not been previously proposed, the results were consistent with a previous investigation of concomitant administration of AZ and SA in humans (Br. J. Clin. Pharmacol. 27, 866, 1989), thereby endorsing utilization of the IPK as a screening tool for renal clearance mechanisms in humans.
Taft
D R
DR
School of Pharmacy, University of Connecticut, USA.
Chapron
D J
DJ
Fournier
D J
DJ
Sweeney
K R
KR
D000086:Acetazolamide / Q000378:metabolism* / Q000494:pharmacology; D000418:Albumins / Q000378:metabolism; D000818:Animals; D004232:Diuretics / Q000378:metabolism* / Q000494:pharmacology; D004347:Drug Interactions; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D010477:Perfusion / Q000379:methods; D011485:Protein Binding; D051381:Rats; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid
D000418:Albumins; D004232:Diuretics; D012459:Salicylates; D000086:Acetazolamide; D020156:Salicylic Acid