Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences.
By low stringency screening of a lambda-Shizosaccharomyces pombe genomic library, we have cloned a GATA factor homologous gene, gaf2+, within a 3.1-kb EcoRI fragment. The gaf2 ORF predicts a protein of M(r) 61 kDa consisting of intronless 564 amino acids corresponding to 1,692 bp. Gaf2 has two zinc-fingers as Urbs1 of Ustilago maydis, whereas most of fungal GATA factors have only one zinc-finger. The separation between two zinc-fingers of Gaf2 is rather long. In addition to gaf2, the sequence analysis revealed a Val-tRNA gene in the 3'-flanking region of gaf2. Northern blot analysis indicated that the gaf2 gene is transcribed constitutively irrespective of the nitrogen source in a medium.
Hoe
K L
KL
Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon, Korea.
Won
M S
MS
Yoo
O J
OJ
Yoo
H S
HS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D050980:GATA Transcription Factors; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012366:RNA, Transfer, Val / Q000032:analysis; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C459859:Fep1 protein, S pombe; D050980:GATA Transcription Factors; D012366:RNA, Transfer, Val; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors