The HTLV-I envelope glycoproteins: structure and functions.
The human T-cell lymphotropic virus type I (HTLV-I) envelope has a structural organization shared by all retroviral envelopes, which contain two mature viral glycoproteins deriving from a common precursor: an external surface protein (SU), associated with a transmembrane protein (TM) responsible for anchoring the SU-TM complex at the cell surface or in the viral envelope. Our understanding of the tertiary structure of these proteins is extremely poor. The intracellular maturation follows the normal cellular secretory pathway, resulting in expression of the mature glycoproteins at the cell surface. The five potential N-glycosylation sites are glycosylated. Most mutations artificially introduced into the glycoproteins result in loss of function, mostly due to abnormal intracellular maturation. This probably indicates a very compact structure of these proteins, where the entire structure is involved in correct conformation. Studies using neutralizing antibodies or mutagenesis have defined functional domains in the SU protein, which is responsible for receptor binding. These domains occur throughout the SU glycoprotein. Sequence analysis of the HTLV-I TM predicts a structure, and probably functions, similar to other retrovirus TMs: involvement of this glycoprotein in the different oligomerization steps leading to a fusogenic SU-TM complex and in the fusion process itself. These features remain to be proven, and it is not yet understood why the free HTLV-I viral particle is not infectious.
Delamarre
L
L
CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.
Rosenberg
A R
AR
Pique
C
C
Pham
D
D
Callebaut
I
I
Dokhélar
M C
MC
D000595:Amino Acid Sequence; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000502:physiology; D013601:T-Lymphocytes / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly
D006023:Glycoproteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins