title: Laminar distribution of MK-801, kainate, AMPA, and muscimol binding sites in cat visual cortex: a developmental study.
abstract: We used quantitative autoradiography to determine whether the development of glutamate receptors correlates with the sensitive period for monocular deprivation in the visual cortex. To study glutamate receptors, we incubated sections of cat visual cortex with tritiated (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10imin e-maleate (MK-801), tritiated kainate, and tritiated amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA). [3H]MK-801 is a noncompetitive ligand for the N-methyl-D-aspartate (NMDA) receptor. [3H]kainate and [3H]AMPA are competitive ligands for non-NMDA receptors. We used [3H]muscimol, which binds to GABAA receptors, so that we would have one control ligand that binds to a nonglutamate receptor. When all layers were combined, the results confirmed our previous studies with homogenate binding. [3H]MK-801 and [3H]kainate binding were significantly greater at 42 days than at earlier or later times. [3H]AMPA and [3H]muscimol binding did not show such a peak. This suggests that MK-801 and kainate binding sites are more likely to be involved in plasticity than are AMPA and muscimol binding sites. In layers 2/3, MK-801 had the greatest age-dependent changes; in layers 5 and 6, kainate binding changed most with age. This suggests that the mechanisms of plasticity may vary with cortical layer.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403, USA.
authors.forename: B
authors.initials: B
authors.lastname: Gordon
authors.forename: D
authors.initials: D
authors.lastname: Pardo
authors.forename: K
authors.initials: K
authors.lastname: Conant
mesh_terms: D000818:Animals; D001665:Binding Sites; D002415:Cats / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D009473:Neuronal Plasticity / Q000502:physiology; D011869:Radioligand Assay; D018091:Receptors, AMPA / Q000032:analysis*; D011963:Receptors, GABA-A / Q000032:analysis*; D018092:Kainic Acid Receptors / Q000032:analysis*; D014793:Visual Cortex / Q000737:chemistry* / Q000254:growth & development
chemical_list: D016291:Dizocilpine Maleate; D018091:Receptors, AMPA; D011963:Receptors, GABA-A; D018092:Kainic Acid Receptors