V beta CDR3 motifs associated with BP recognition are enriched in OX-40+ spinal cord T cells of Lewis rats with EAE.
Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in V beta 8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in V beta 8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX-40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.
Buenafe
A C
AC
Neuroimmunology Research, Portland V.A. Medical Center, Oregon, USA.
Weinberg
A D
AD
Culbertson
N E
NE
Vandenbark
A A
AA
Offner
H
H
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism*; D005260:Female; D008562:Membrane Glycoproteins; D004676:Myelin Basic Protein / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D048069:Tumor Necrosis Factors
D000954:Antigens, Surface; D001324:Autoantigens; D008562:Membrane Glycoproteins; D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell; D018124:Receptors, Tumor Necrosis Factor; C504587:Tnfsf4 protein, rat; D048069:Tumor Necrosis Factors