The detection of Mycoplasma pneumoniae in nasal polyps.
The aetiology and microbial flora of nasal polyps is not well understood. No study in the literature has reported an association between the sub-bacterium Mycoplasma pneumoniae and nasal polyps. We have developed an assay method using the Polymerase Chain Reaction (PCR) to amplify a specific region of the M. pneumoniae DNA in extracts of clinical samples using species-specific primers designed to a region of the 16S rRNA. The presence of M. pneumoniae was detected in 13/14 (93%) nasal polyps, in 4/5 (80%) rhinosinusitis mucosal samples but only in 1/7 (14%) of control samples (obstructive turbinates). An epidemic of infections due to M. pneumoniae is expected to occur in 1995. We believe this assay could form the basis of a rapid technique for M. pneumoniae detection. We also propose that the presence of M. pneumoniae may be of importance in the aetiology of nasal polyps.
Gurr
P A
PA
Royal National Throat, Nose and Ear Hospital, London, UK.
Chakraverty
A
A
Callanan
V
V
Gurr
S J
SJ
D015139:Blotting, Southern; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D009298:Nasal Polyps / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012852:Sinusitis / Q000382:microbiology*
D017931:DNA Primers