Nuclear degeneration in epidermal cells of drying onion bulb leaf bases.
We tested the null hypothesis 'that activated nuclei and nucleoli in outer-epidermal cells of newly exposed equatorial tissue of the turgid leaf bases of white onions (exposed to the ambient atmosphere by removal of two dry and two turgid leaf bases) remained in that state as the tissue dried' by following nuclear macromolecules (total nucleic acid, DNA, RNA, total protein, histone, and non-histone protein; compared with T0 = 100%) and nucleolar morphologies over a 5-day period. The nuclei became activated within 6 h and remained in that state for 2-3 days [increases in RNA, non-histone protein, and volume of major nucleoli occurred by T12 (about 191, 177, and 289%, respectively) and appearance of the minor nucleoli between T12 and T24 (activation of silent rRNA cistrons)]. Combined nucleolar (major and minor) volumes decreased to 228% by T24 and to 150% by T48. Minor nucleoli were visible at T24 and T48. DNA (DAPI) remained unchanged over that period of time. At the T96 sampling, all nuclear indices had decreased to levels below those obtained at the time of exposure to the ambient atmosphere; minor rRNA cistrons had became silent genes; nuclear volume was about 89% of the original volume; and, nucleolar volume (major nucleoli) was about 93%. The percentages for nuclear indices at T120 were DNA, 85% of T0; RNA, 35%; histone, 87%; non-histone protein, 47%; nuclear volume, 81%; and nucleolar volume, 67%. Of interest is the lack of change in major nucleolar morphologies between T96 and T120 although they decreased in volume during that period. We infer that the karyoskeleton (nuclear matrix) had undergone irreversible degeneration after T48 and that the cells had passed the point-of-no-return in the senescence pathway by T120. We propose that this model for cell senescence and death (drying of turgid leaf bases to form the dry, dead outer covering of the bulbs) simulates post-harvest storage conditions and will prove helpful to those studying cellular senescence mechanisms and associated host-pathogen interactions in plants.
Bhattacharya
P K
PK
Department of Biology, Indiana University Northwest, Gary 46408, USA.
Pappelis
A J
AJ
Karagiannis
C S
CS
D000490:Allium / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D005456:Fluorescent Dyes; D006657:Histones / Q000378:metabolism; D007211:Indoles; D008954:Models, Biological; D009696:Nucleic Acids / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013997:Time Factors; D014867:Water / Q000378:metabolism*
D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; D009696:Nucleic Acids; D010940:Plant Proteins; D014867:Water; C007293:DAPI; D012313:RNA; D004247:DNA