Sensitive high-performance liquid chromatographic method for the determination of 2-phenylethylamine in human urine.
A new sensitive high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed for the determination of 2-phenylethylamine (PEA) in human urine. The analytical procedure involved a simple extraction of the analyte from urine, followed by precolumn derivatisation of the sample with o-phthalaldehyde. The HPLC separation was performed under isocratic conditions using an Erbasil S C18 (250 x 4.0 mm I.D., particle size 3 microns) reversed-phase column. The limit of quantitation was 0.5 ng of PEA/ml of urine. The method showed good linearity, accuracy and precision data in the concentration range 0.5-200 ng/ml of urine. The method was successfully applied to the determination of PEA urinary excretion in Parkinsonian patients after oral administration of the monoamine oxidase B (MAO-B) inhibitor, selegiline.
La Croix
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R&D/Pharmacokinetics and Metabolism Department, Pharmacia, SpA, Nerviano, Milan, Italy.
Dostert
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Strolin Benedetti
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D000368:Aged; D000978:Antiparkinson Agents / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D008996:Monoamine Oxidase Inhibitors / Q000493:pharmacokinetics / Q000627:therapeutic use; D010300:Parkinson Disease / Q000188:drug therapy / Q000652:urine; D010627:Phenethylamines / Q000737:chemistry / Q000652:urine*; D011619:Psychotropic Drugs / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012642:Selegiline / Q000493:pharmacokinetics / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde / Q000737:chemistry
D000978:Antiparkinson Agents; D007202:Indicators and Reagents; D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; D011619:Psychotropic Drugs; D012642:Selegiline; C029261:phenethylamine; D009764:o-Phthalaldehyde