Capillary zone electrophoresis of collagen type I CNBr peptides in acid buffers.
Collagen type-I CNBr peptides were separated under acidic conditions by capillary electrophoresis. Separation conditions were: 100 mM phosphate buffer pH 2.5, 50 cm x 50 microns capillary (placed in a cartridge), 8 kV, running time 30-45 min, detection by UV at 200 nm. The peptides were separated strictly by their molecular mass and the overall pattern was well comparable to RP-HPLC separations of these analytes. It is proposed that the separation mechanism may involve hydrophobic sorptions to the capillary wall.
Novotná
J
J
Department of Medical Chemistry and Biochemistry, Charles University, 2nd Medical School, Prague, Czech Republic.
Deyl
Z
Z
Miksík
I
I
D000143:Acids / Q000737:chemistry; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry*; D003488:Cyanogen Bromide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000143:Acids; D002021:Buffers; D010446:Peptide Fragments; D003094:Collagen; D003488:Cyanogen Bromide