Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (antenna) proteins: bioluminescence effects of the aliphatic additive.
The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1. Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects can be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, where the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on-off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme. These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found for the protein-protein interaction in the absence of the aliphatic component.
Petushkov
V N
VN
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA.
Ketelaars
M
M
Gibson
B G
BG
Lee
J
J
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D004227:Dithionite; D005486:Flavin Mononucleotide / Q000378:metabolism; D007700:Kinetics; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D008961:Models, Structural; D010776:Photobacterium / Q000201:enzymology*; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry; D014733:Vibrio / Q000201:enzymology*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; C026648:lumazine protein, Photobacterium; C054562:yellow fluorescent protein, Bacteria; D004227:Dithionite; D005486:Flavin Mononucleotide; D008156:Luciferases