Immunoenzymatic detection of staphylococcal enterotoxins: international interlaboratory study.
An international interlaboratory study was performed by 13 laboratories to validate a commercially available, rapid enzyme immunoassay for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. The 5 enterotoxin serotypes were detected at a level of 0.5 ng/g in mushrooms and ravioli, 0.8 ng/g in meat, 1 ng/mL in milk, and 1.5 ng/g in raw milk cheese when these foods were artificially contaminated with enterotoxin A. Enterotoxins A, B, C, D, or E were also detected in culture supernatants with no protein A interference when normal rabbit serum was used. This method was validated by the French Normalization Agency for the identification of staphylococcal enterotoxins in foods and culture fluids.
Lapeyre
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C
Centre National D'Etudes Vétérinaires et Alimentaires, CNEVA Paris-LCHA, France.
de Solan
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MN
Drouet
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X
D000918:Antibody Specificity; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis; D005504:Food Analysis / Q000592:standards; D005506:Food Contamination; D007124:Immunoenzyme Techniques; D007391:International Cooperation; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D013202:Staphylococcal Food Poisoning / Q000175:diagnosis* / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000378:metabolism*
D003470:Culture Media; D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic