Effects of ionizing- and UV B-radiation on proteins controlling cell cycle progression in human cells: comparison of the MCF-7 adenocarcinoma and the SCL-2 squamous cell carcinoma cell line.
MCF-7 and SCL-2 cells were irradiated with UV B-radiation or with 137Cs gamma-radiation, in order to investigate cell cycle checkpoint control mechanisms. Effects of both qualities of radiation were investigated for the two cell lines in regard to p53 protein levels, and alterations in Cdk1 (cyclin dependent kinase 1) and Cdk2 phosphorylation were monitored. SCL-2 cells constitutively overexpressed a form of p53 protein whose abundance remained unchanged after irradiation, whereas MCF-7 cells expressed wild type p53 whose abundance increased after irradiation. Accordingly, MCF-7 cells showed a strong G1 phase arrest, whereas SCL-2 cells were only delayed in S phase (after UV B-irradiation) and arrested in G2 phase (after gamma-irradiation and UV B-irradiation), as monitored by flow cytometry. In MCF-7 cells increased p53 levels were observed for up to 30 h after gamma-irradiation and up to 20 h after UV B-irradiation. Only in SCL-2 cells was there a significant radiation induced inactivation of Cdk1 by hyperphosphorylation. This effect was prevented by culturing cells in the presence of caffeine after irradiation. After UV B-irradiation the inactivation of Cdk1 was less pronounced and only partially diminished in the presence of caffeine. No alteration in Cdk2 phosphorylation was observed after irradiation in either cell line.
Hain
J
J
Bundesamt für Strahlenschutz, Institut für Strahlenhygiene, Oberschleissheim, Germany.
Weller
E M
EM
Jung
T
T
Burkart
W
W
D000230:Adenocarcinoma / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D042846:CDC2-CDC28 Kinases; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle / Q000528:radiation effects*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D014466:Ultraviolet Rays
D016159:Tumor Suppressor Protein p53; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases