title: Expansion of bronchial epithelial cell populations by in vitro culture of explants from dysplastic and histologically normal sites.
abstract: The genetic and phenotypic properties of cells which ultimately give rise to carcinoma of the lung are not well defined in part because of unavailability of preneoplastic cells from well-characterized dysplastic sites. In order to expand bronchial epithelial cell populations from patients at high risk for lung cancer, endobronchial biopsy specimens were explanted onto collagen- and fibronectin-coated dishes and cultured in serum-free, chemically defined media. One hundred forty-nine biopsy pairs were obtained from smokers and from healthy volunteers for culture and histologic evaluation. The histologic appearances of mucosa adjacent to the site of the cultured biopsies ranged from normal through varying degrees of noninvasive squamous dysplasia to invasive carcinoma. Confluent monolayers of pure epithelial cells were obtained from 68% of the cultured explants. Sites exhibiting high-grade dysplasia were 51% more likely to yield successful cultures than sites exhibiting normal histology (13 of 14 cultures successful versus 52 of 83 cultures successful, P < 0.02). Cultures had a maximum proliferative life span of 81 days and none of the cultures spontaneously became immortalized. Immunolabeling studies revealed that all cultured epithelial cells, regardless of the in situ histologic appearances of the mucosa at the biopsy site, strongly expressed keratin and epidermal growth factor receptor, weakly expressed transferrin receptor and human folate receptor, and were negative for neural cell adhesion molecule and human leukocyte antigen DR (HLADR). Ploidy and karyotypic analyses were performed in a limited number of explants from normal and dysplastic sites and all were found to be diploid without karyotypic abnormality. We conclude that pure bronchial epithelial cell populations can be routinely expanded from histologically normal and dysplastic sites by tissue culture of biopsy explants and that the expanded cell populations may represent a library of normal and preneoplastic cells which are suitable for immunophenotypic, ploidy, genetic, or functional analyses.
authors.affiliation: University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver 80262, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Franklin
authors.forename: J M
authors.initials: JM
authors.lastname: Folkvord
authors.forename: M
authors.initials: M
authors.lastname: Varella-Garcia
authors.forename: T
authors.initials: T
authors.lastname: Kennedy
authors.forename: S
authors.initials: S
authors.lastname: Proudfoot
authors.forename: R
authors.initials: R
authors.lastname: Cook
authors.forename: E C
authors.initials: EC
authors.lastname: Dempsey
authors.forename: K
authors.initials: K
authors.lastname: Helm
authors.forename: P A
authors.initials: PA
authors.lastname: Bunn
authors.forename: Y E
authors.initials: YE
authors.lastname: Miller
mesh_terms: D001980:Bronchi / Q000166:cytology* / Q000473:pathology; D001984:Bronchial Neoplasms / Q000473:pathology*; D001999:Bronchoscopy; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002871:Chromosome Banding; D004847:Epithelial Cells; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D011003:Ploidies; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology