Crystallization and preliminary X-ray studies of ornithine decarboxylase from Trypanosoma brucei.
Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized by the vapor diffusion method using PEG 3350 as a precipitant. The crystals belong to the monoclinic space group P2(1) and have cell constants of a = 66.3 angstroms, b = 151.8 angstroms, c = 83.7 angstroms, and beta = 101.2 degrees. While larger crystals are twinned, smaller crystals (0.4 x 0.3 x 0.05 mm3) are single.
Grishin
N V
NV
Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 75235-9041, USA.
Osterman
A L
AL
Goldsmith
E J
EJ
Phillips
M A
MA
D000818:Animals; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D009955:Ornithine Decarboxylase / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D009955:Ornithine Decarboxylase