title: Changes in poly(A) tail length of arginine vasotocin messenger ribonucleic acid in the hypothalamus of water-deprived chickens.
abstract: The effects of water deprivation on the secretion of vasotocin (AVT) and expression of the AVT gene were studied in White Leghorn cockerels. Animals deprived of water for 4 days were compared with normally hydrated controls. Blood samples were obtained for measurements of plasma osmolality and AVT levels, and the hypothalamus was collected for extraction of total cellular RNA. A 519-bp AVT cDNA was prepared by reverse transcriptase-polymerase chain reaction and a 209-bp PstI/EcoRI restriction fragment from the 3' region of the fowl AVT cDNA was used as a probe for Northern blot analysis. Plasma osmolality and AVT levels in dehydrated birds were about 30 and 350% greater, respectively, than those in normally hydrated controls. The quantity of hypothalamic AVT mRNA was 2. 3-fold greater in water-deprived birds compared to controls. The size of the hypothalamic AVT transcript was about 100-bp longer in the water-deprived birds. As determined by RNase H treatment in the presence and absence of oligo(dT)12-18, the increase in mean size of the AVT mRNA in dehydrated animals was due to a longer poly(A) tract. Our results indicate that osmotic stress up-regulates expression of the AVT gene and increases the accumulation of AVT mRNA in the hypothalamus. This accumulation may, in part, be due to lengthening of the AVT mRNA poly(A) tail which is a general mechanism associated with stabilization of vertebrate mRNAs.
authors.affiliation: Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, 72205, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Chaturvedi
authors.forename: Z
authors.initials: Z
authors.lastname: Zheng
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: L E
authors.initials: LE
authors.lastname: Cornett
authors.forename: T I
authors.initials: TI
authors.lastname: Koike
mesh_terms: D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens / Q000097:blood / Q000502:physiology*; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D007031:Hypothalamus / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011897:Random Allocation; D011933:Reagent Kits, Diagnostic; D016914:Ribonuclease H / Q000378:metabolism; D014668:Vasotocin / Q000097:blood / Q000235:genetics* / Q000276:immunology; D014868:Water Deprivation / Q000502:physiology*
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D016914:Ribonuclease H; D014668:Vasotocin