Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes.
Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present in human sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptides from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.
Mennuni
C
C
Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia, Rome, Italy.
Santini
C
C
Dotta
F
F
Farilla
L
L
Di Mario
U
U
Fierabracci
A
A
Bottazzo
G
G
Cortese
R
R
Luzzago
A
A
D000293:Adolescent; D000328:Adult; D000915:Antibody Affinity; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes / Q000032:analysis; D004279:DNA, Viral; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics*; D005260:Female; D015723:Gene Library; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000276:immunology*
D015342:DNA Probes; D004279:DNA, Viral; D000939:Epitopes; D010455:Peptides