title: Lymphocyte subpopulations in the mammary gland of the goat.
abstract: Mammary glands of pregnant, lactating and resting goats were studied by immunohistochemistry for lymphocyte subpopulations using a panel of monoclonal antibodies. All T lymphocyte subpopulations that may have a role in the immune response, CD2+, CD4+, CD8+ and gamma delta T cells and subsets, were present in the mammary gland and were noted to increase in number progressively during pregnancy, decrease significantly during lactation, and then moderately increase during the resting period. CD4+ cells, the predominant cell type in the mammary gland, were located mainly in the connective tissue, whereas CD2+, CD8+ and TcR1-N24+ cells were predominant in the intraepithelial areas. TcR1-N6+ cells were detected almost exclusively during pregnancy, being localized mainly in the connective tissue. Their proportion decreased markedly following parturition. Very few WC1-N3+ and -N4+ cells were detected in the mammary gland. It is suggested that the majority of gamma delta T lymphocytes in the mammary gland of the goat are CD2+ CD8+ WCl-, a distinctive subset from that of the WCl+ subset in peripheral blood.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. hismail@vetmed.hokudai.ac.jp
authors.forename: H I
authors.initials: HI
authors.lastname: Ismail
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kon
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: T
authors.initials: T
authors.lastname: Iwanaga
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001706:Biopsy; D005260:Female; D006041:Goats; D007150:Immunohistochemistry; D007774:Lactation; D016131:Lymphocyte Subsets / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000276:immunology*; D011247:Pregnancy; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016692:Receptors, Antigen, T-Cell, gamma-delta