Overproduction of recombinant ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus sp. strain PCC6301 in glucose-controlled high-cell-density fermentations by Escherichia coli K-12.
A predictive and feedback glucose feed controller, previously developed for nutrient-sufficient growth of Escherichia coli to high cell densities, was used to produce large quantities of a heterologous, cyanobacterial recombinant hexadecameric (L8S8) protein, ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) in E. coli. Culture and plasmid stability conditions were optimized to yield the production of approximately 1 g of soluble, active recombinant RubisCO per liter. Recombinant RubisCO also was produced in lactose-induced high-cell-density fermentation of E. coli K-12.
Kleman
G L
GL
Department of Microbiology, Ohio State University, Columbus 43210, USA.
Horken
K M
KM
Tabita
F R
FR
Strohl
W R
WR
D000458:Cyanobacteria / Q000201:enzymology*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology*; D007785:Lactose / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis*
D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase; D005947:Glucose; D007785:Lactose