Medullary respiratory neuronal activity modulated by stimulation of the fastigial nucleus of the cerebellum.
The ability of the rostral fastigial nucleus (FNr) of the cerebellum to modulate medullary respiratory neuronal activity was examined in 17 anesthetized, paralyzed and ventilated cats. A bipolar stimulating electrode was positioned into the FNr and tungsten microelectrodes used to record units within the nucleus tractus solitarius (NTS), nucleus ambiguus (NA) and nucleus retroambigualis (NRA). Transient stimuli (< 150 microA, 5-200 Hz) were delivered during inspiration or expiration, and the effects noted on medullary neuronal activity and the phrenic neurogram. The results showed that FNr stimulation: (1) modulated inspiratory and expiratory neuronal (ramp-, early- and late-inspiratory and stage I and II expiratory) discharges recorded from the NTS, NA and NRA (n = 67, 14 and 28) when stimuli (> or = 20-50 Hz) were delivered during either the inspiratory or expiratory phases; (2) terminated the burst durations of inspiratory (77%) and expiratory (94%) neurons with stimulus-response latencies of 28.2 +/- 3.1 ms (inspiratory) and 29.4 +/- 3.6 ms (expiratory); (3) elicited changes in phrenic neurogram concomitant with the effects noted on medullary neuronal activities; (4) failed to change heart rate and arterial blood pressure; and (5) did not affect medullary neuronal and phrenic nerve activity following kainic acid injection into the FNr. We conclude that activation of the FNr (likely its cell bodies) can modulate the respiratory output via influences on medullary respiratory-related neurons. The primary cerebellar effect across all sub-types of respiratory neurons was early termination.
Xu
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Department of Physiology, University of Kentucky, Lexington 40536, USA.
Frazier
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D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure; D002415:Cats; D002529:Cerebellar Nuclei / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology; D002698:Chloralose / Q000494:pharmacology; D004525:Efferent Pathways / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D006339:Heart Rate; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology; D008845:Microinjections; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology; D010507:Periodicity; D010791:Phrenic Nerve / Q000166:cytology / Q000502:physiology; D012119:Respiration / Q000187:drug effects / Q000502:physiology; D012125:Respiratory Center / Q000166:cytology* / Q000502:physiology; D017552:Solitary Nucleus / Q000166:cytology* / Q000502:physiology; D013874:Thiopental / Q000494:pharmacology
D018686:Anesthetics, Intravenous; D002698:Chloralose; D013874:Thiopental; D007608:Kainic Acid