title: Preliminary comparison of mineralizing multilayer cultures formed by primary fetal bovine mandibular osteoblasts grown on titanium, hydroxyapatite, and glass substrates.
abstract: Bone formation at implant surfaces may be directly influenced by effects of the implant material on osteoblast behavior. Cell culture models of osteoblast physiology may be used to investigate the interaction of osteoblastic cells with various surfaces. In this study, primary cultured fetal bovine mandibular osteoblastic cells were cultured on titanium, ceramic hydroxyapatite, and glass coverslip surfaces to allow for the comparison of the mineralizing matrix elaborated by osteoblasts grown on different implant material surfaces. Morphologic and immunohistochemical analysis revealed the similar formation of multilayered, mineralizing cultures on these three surfaces. The qualitative similarity of the matrix formed on these culture surfaces may reflect similar qualitative in vivo responses of bone to titanium and hydroxyapatite implants.
authors.affiliation: Department of Prosthodontics, University of North Carolina, Chapel Hill 27599-7450, USA.
authors.forename: P K
authors.initials: PK
authors.lastname: Yliheikkilä
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: W W
authors.initials: WW
authors.lastname: Ambrose
authors.forename: C A
authors.initials: CA
authors.lastname: Suggs
authors.forename: D A
authors.initials: DA
authors.lastname: Felton
authors.forename: L F
authors.initials: LF
authors.lastname: Cooper
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000166:cytology / Q000502:physiology; D002113:Calcification, Physiologic; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D003470:Culture Media; D015921:Dental Implants; D017886:Durapatite / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D007150:Immunohistochemistry; D008334:Mandible / Q000166:cytology / Q000502:physiology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D003470:Culture Media; D015921:Dental Implants; D017886:Durapatite; D014025:Titanium