title: Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins.
abstract: Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.
authors.affiliation: Department of Biochemistry, Stockholm University, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Mingarro
authors.forename: P
authors.initials: P
authors.lastname: Whitley
authors.forename: M A
authors.initials: MA
authors.lastname: Lemmon
authors.forename: G
authors.initials: G
authors.lastname: von Heijne
mesh_terms: D000409:Alanine / Q000235:genetics*; D006021:Glycophorins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006021:Glycophorins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D000409:Alanine