title: Hepatitis C virus infection-associated markers in sera from blood donors in Surabaya, Indonesia.
abstract: Among 2,233 sera obtained from volunteer blood donors, 259 (11.6%) showed elevated alanine aminotransferase (ALT) levels. A second-generation enzyme-linked immunosorbent assay (ELISA) revealed that 23 (8.9%) of the 259 sera were positive for antibodies against hepatitis C virus (HCV), whereas only 9 (1.4%) of 646 sera randomly collected from blood donors with normal ALT levels were positive (P < 0.001). The overall prevalence of anti-HCV antibodies among blood donors was estimated to be 2.3%. HCV RNA was detected in 19 (83%) of the 23 anti-HCV-positive sera with elevated ALT levels, and 8 (89%) of the 9 sera with normal ALT levels. Among the anti-HCV-positive sera, IgM anti-HCV was detected in 5 (22%) of 23 sera with elevated ALT levels and in 2 (22%) of 9 sera with normal ALT levels. All of the IgM anti-HCV-positive sera were positive for HCV RNA, irrespective of ALT levels.
authors.affiliation: Tropical Disease Research Center, Airlangga University, Surabaya, Indonesia.
authors.forename: S
authors.initials: S
authors.lastname: Darmadi
authors.lastname: Soetjipto
authors.forename: R
authors.initials: R
authors.lastname: Handajani
authors.forename: M I
authors.initials: MI
authors.lastname: Lusida
authors.lastname: Soemarto
authors.forename: H
authors.initials: H
authors.lastname: Sakugawa
authors.forename: S
authors.initials: S
authors.lastname: Ishido
authors.forename: H
authors.initials: H
authors.lastname: Hotta
mesh_terms: D000410:Alanine Transaminase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D012367:RNA, Viral / Q000097:blood*
chemical_list: D015415:Biomarkers; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D000410:Alanine Transaminase