Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro.
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.
Mizushina
Y
Y
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Chiba-ken, Japan.
Tanaka
N
N
Yagi
H
H
Kurosawa
T
T
Onoue
M
M
Seto
H
H
Horie
T
T
Aoyagi
N
N
Yamaoka
M
M
Matsukage
A
A
Yoshida
S
S
Sakaguchi
K
K
D000818:Animals; D002417:Cattle; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013942:Thymine Nucleotides / Q000378:metabolism
D003902:Detergents; D005227:Fatty Acids; D008041:Linoleic Acids; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D019787:Linoleic Acid; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate