Substance P enhances antigen-evoked mediator release from human nasal mucosa.
Exogenous substance P (10-80 nmol/ml) induced a dose-dependent increase in nasal symptoms in asymptomatic allergics with rhinitis (n = 15) and controls (n = 8), but did not release any mediators. However, comparing the antigen-evoked release of mediators into nasal secretions with that of a substance P-pretreated antigen challenge, we found a significant enhancement of kinins, TAME esterase activity (p < 0.05-0.01), and histamine (p < 0.001, NS) 10-20 min after antigen challenge. These results suggest 1) that substance P-induced increase in nasal congestion is mediated through direct neurokinin receptor activation independently of mast cell activation, and 2) that during the allergic reaction there is a substance P-mast cell interaction that enhances the mediator response to nasal allergen challenge.
Baumgarten
C R
CR
Clinical Immunology and Asthma ODP, Freie Universität Berlin, Germany.
Witzel
A
A
Kleine-Tebbe
J
J
Kunkel
G
G
D000328:Adult; D000485:Allergens / Q000008:administration & dosage*; D001920:Bradykinin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D009297:Nasal Mucosa / Q000187:drug effects* / Q000276:immunology*; D010447:Peptide Hydrolases / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000209:etiology / Q000276:immunology; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology
D000485:Allergens; D013373:Substance P; D007073:Immunoglobulin E; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase; D001920:Bradykinin