title: A 31P NMR study of preconditioned isolated perfused rat heart exposed to intermittent ischemia.
abstract: Exposure to a short ischemic period (ischemic preconditioning, IP) will protect the heart from damage following a subsequent longer ischemic episode. The aim of the study was to test whether IP is cardioprotective in the setting of repeated ischemia-reperfusion cycles. Thus, Langendorff-perfused hearts, exposed to IP, were subjected to three consecutive ischemia-reperfusion (10/15 min) cycles. Myocardial energetics, manifested by 31P NMR spectroscopy, was correlated with hemodynamics. ATP recovery was significantly higher for the IP group compared with control (P < 0.02) during reperfusions. However, there was no significant difference in ATP recovery during the three ischemic intervals. The supernormal recovery of phosphocreatine recorded during reperfusion was lower for the IP group (approximately 120%) compared with control (approximately 135%, P < 0.065). Better recovery of the left ventricular-developed pressure was noted during reperfusions for the IP group and became significant only during the last reperfusion (86% versus 68%, P < 0.025). In conclusion, the above results support prolonged IP cardioprotection.
authors.affiliation: Human Biology Research Center, Joseph Lunenfeld Cardiac Surgery Research Center, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Sharoni
authors.forename: A
authors.initials: A
authors.lastname: Olivson
authors.forename: M
authors.initials: M
authors.lastname: Chandra
authors.forename: G
authors.initials: G
authors.lastname: Merin
authors.forename: G
authors.initials: G
authors.lastname: Uretzky
authors.forename: J B
authors.initials: JB
authors.lastname: Borman
authors.forename: R
authors.initials: R
authors.lastname: Chisin
authors.forename: H
authors.initials: H
authors.lastname: Schwalb
authors.forename: E
authors.initials: E
authors.lastname: Berman
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003326:Coronary Circulation; D004734:Energy Metabolism; D006339:Heart Rate; D006863:Hydrogen-Ion Concentration; D019157:Ischemic Preconditioning, Myocardial; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D010759:Phosphorus Isotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left; D017725:Ventricular Pressure
chemical_list: D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D000255:Adenosine Triphosphate