Glycine produced pressor responses when microinjected in the pressor areas of pons and medulla in cats.
In 24 cats under chloralose/urethane anesthesia changes of systemic arterial pressure (SAP) and sympathetic vertebral nerve activities (VNA) were induced by microinjection of glycine (Gly, 1.0 M, 50 nl) into the pressor areas of the rostral pons, i.e., locus coeruleus-parabrachial nucleus (LC-PBN), nucleus of gigantocellular tegmental field-lateral tegmental field (FTG-FTL), and dorsomedial (DM) and ventrolateral (VLM) medulla. The effects were compared with those induced by microinjection of sodium glutamate (Glu, 0.25 M, 50 nl) into the same sites. In about 60% of the injections Gly produced increases in SAP and VNA similar to that of Glu. The increase in SAP was greater in VLM, while the increase in VNA was more marked in DM. In the rest of microinjections Gly and Glu produced changes of SAP and VNA in different combinations. The latency of Gly-induced increases in SAP and VNA was 1 to 3 s longer than that induced by Glu. Our findings show that although Gly is classified as an inhibitory transmitter, it often produced excitation of the pressor neurons in the pons and medulla similar to that of Glu. Whether Gly acts through the same cardiovascular neurons that respond to Glu or through activation of different kinds of neurons remains to be elucidated.
Wu
W C
WC
Institute of Biomedical Sciences, Academia Sinica, Taiwan, The Republic of China.
Chen
S Y
SY
Kuo
J S
JS
Chai
C Y
CY
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology*; D008125:Locus Coeruleus / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D008845:Microinjections; D011149:Pons / Q000502:physiology*; D013233:Stellate Ganglion / Q000166:cytology / Q000502:physiology; D013268:Stimulation, Chemical; D017779:Sympathetic Fibers, Postganglionic / Q000502:physiology
D018698:Glutamic Acid; D005998:Glycine