The pyridoindole antioxidant stobadine prevents alloxan-induced lipid peroxidation by inhibiting its propagation.
Under in vitro conditions, the pyridoindole stobadine inhibited alloxan-induced lipid peroxidation in a model biological membrane with the efficacy comparable with that of the standard Trolox. Intermediary alloxan radicals and hydroxyl radicals were not directly involved in the process of lipid peroxidation, however, the presence of iron chelate was a necessary prerequisite. Since stobadine did not affect the kinetics of alloxan redox-cycling in the presence of GSH, we suggest that the protective action of stobadine against the alloxan-induced lipid peroxidation was mediated predominantly by its ability to quench peroxyl radicals, inhibiting thus the propagation stage of the oxidative damage. The results also indicate that toxic effects of alloxan may well be mediated by mechanism(s) not involving hydroxyl radicals.
Stefek
M
M
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Trnkova
Z
Z
D000496:Alloxan / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001463:Barbiturates / Q000378:metabolism; D002243:Carbolines / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D001463:Barbiturates; D002243:Carbolines; C004718:dialuric acid; D000496:Alloxan; D006861:Hydrogen Peroxide; C100106:dicarbine