An S-locus-related gene in Arabidopsis encodes a functional kinase and produces two classes of transcripts.
ARK1 is a vegetatively expressed receptor protein kinase gene isolated from Arabidopsis thaliana based on its sequence similarity to Brassica genes involved in pollen-stigma signaling and the self-incompatibility response. This paper shows that the kinase domain of ARK1 autophosphorylates on serine and threonine residues when expressed as a recombinant fusion protein. ARK1 produces a 2.9 kb transcript encoding a transmembrane receptor protein kinase and a 1.4 kb transcript encoding the receptor domain alone. Constitutive high-level expression of ARK1 transcripts in transgenic Arabidopsis resulted in severe stunting and also disrupted normal cellular expansion and differentiation.
Tobias
C M
CM
Division of Biological Sciences, Cornell University, Ithaca, NY 14853, USA.
Nasrallah
J B
JB
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010641:Phenotype; D010766:Phosphorylation; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D018749:RNA, Plant; D011494:Protein Kinases