title: Peroxidase-catalyzed generation of catechin oligomers that inhibit glucosyltransferase from Streptococcus sobrinus.
abstract: Oolong tea extract (OTE) and the purified polymeric polyphenols from OTE have been found to inhibit glucosyltransferase (GTase) of mutans streptococci. In view of the partial fermentation characteristic of oolong tea, we describe here an in vitro model reaction system to produce partially fermented products of D-(+)-catechin or green tea extract (GTE) using horseradish peroxidase. A dimeric catechin molecule was identified as dehydro-dicatechin A by instrumental analyses. The molecular size of some oligomeric catechins was estimated by the elution profile with HPLC. These catechin oligomers markedly inhibited GTase from Streptococcus sobrinus 6715. As the degree of polymerization of catechin or GTE increased, GTase was inhibited more effectively. These results suggest that polymeric polyphenols found in OTE are synthesized by partial fermentation due to oxidases/peroxidases present in tea leaves.
authors.affiliation: Department of Oral Microbiology, Osaka University Faculty of Dentistry, Suita-Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hamada
authors.forename: M
authors.initials: M
authors.lastname: Kontani
authors.forename: H
authors.initials: H
authors.lastname: Hosono
authors.forename: H
authors.initials: H
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Ooshima
authors.forename: T
authors.initials: T
authors.lastname: Mitsunaga
authors.forename: I
authors.initials: I
authors.lastname: Abe
mesh_terms: D001422:Bacterial Adhesion; D002392:Catechin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D003731:Dental Caries / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D006801:Humans; D010544:Peroxidases / Q000378:metabolism*; D011108:Polymers; D016986:Streptococcus sobrinus / Q000201:enzymology* / Q000472:pathogenicity / Q000502:physiology; D013662:Tea / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D011108:Polymers; D013662:Tea; D002392:Catechin; D010544:Peroxidases; D005964:Glucosyltransferases