Met-Lys-bradykinin-Ser, the kinin released from human kininogen by human pepsin.
This study was carried out to show the site in kininogen, using synthetic substrates, that is cleaved by a purified human pepsin component with a molecular weight of 35,000 daltons. The study used 4 (four) intramolecularly quenched fluorogenic substrates containing N- and C-terminal sequences around the methionyl-lysyl-bradykinin (MLBK) region of kininogen: Abz-LMKRP-Eddnp, Abz-MISLMKRP-Eddnp, Abz-FRSSR-Eddnp and Abz-RPPGFSPFRSSRQ-Eddnp. The hydrolysis on N-terminal sequences Abz-LMKRP-Eddnp and Abz-MISLMKRP-EDDnp occurred at L-M linkage and on C-terminal sequences Abz-FRSSR-Eddnp, and Abz-RPPGFSPFRSSRQ-Eddnp occurred at S-S linkage. The released peptide Abz-RPPGFSPFRS was able to contract rat uterus muscle. The results suggest that Met-Lys-Bradykinin-Ser, should be the peptide released from human kininogen by a purified human pepsin component.
Silva
R A
RA
Disciplina de Bioquímica, DCB, Faculdade de Medicina do Triângulo Mineiro, Uberaba, MG, Brazil.
Chagas
J R
JR
Juliano
L
L
Hial
V
V
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007704:Kininogens / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D010434:Pepsin A / Q000494:pharmacology*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000502:physiology
D007704:Kininogens; C103782:methionyl-lysyl-bradykinin-serine; C018103:bradykinin, Met-Lys-; D010434:Pepsin A; D001920:Bradykinin