Serum rotavirus neutralizing-antibody titers compared by plaque reduction and enzyme-linked immunosorbent assay-based neutralization assays.
Comparisons in rotavirus neutralizing-antibody responses were made with sera collected from vaccinated infants. The methods were a plaque reduction assay and a new enzyme-linked immunosorbent assay-based neutralization assay. Agreement of 94% was found in detecting at least fourfold seroresponses, and correlation coefficients between titers obtained by the two methods showed excellent agreement, indicating that either could be used reliably.
Ward
R L
RL
James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219, USA.
Kapikian
A Z
AZ
Goldberg
K M
KM
Knowlton
D R
DR
Watson
M W
MW
Rappaport
R
R
D000914:Antibodies, Viral / Q000097:blood*; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D009500:Neutralization Tests / Q000379:methods* / Q000706:statistics & numerical data; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000494:pharmacology; D010948:Viral Plaque Assay / Q000379:methods* / Q000706:statistics & numerical data; D014765:Viral Vaccines / Q000494:pharmacology
D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines