title: Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene.
abstract: The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV polyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.
authors.affiliation: Department of Zoology, National Taiwan University, Taipei.
authors.forename: C M
authors.initials: CM
authors.lastname: Chou
authors.forename: C J
authors.initials: CJ
authors.lastname: Huang
authors.forename: C F
authors.initials: CF
authors.lastname: Lo
authors.forename: G H
authors.initials: GH
authors.lastname: Kou
authors.forename: C H
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000472:pathogenicity; D000077183:Occlusion Body Matrix Proteins; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification*; D015678:Viral Structural Proteins
chemical_list: D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus