title: Evidence that the initial up-regulation of phosphatidylcholine biosynthesis in free cholesterol-loaded macrophages is an adaptive response that prevents cholesterol-induced cellular necrosis. Proposed role of an eventual failure of this response in foam cell necrosis in advanced atherosclerosis.
abstract: Macrophages in atherosclerotic lesions accumulate free cholesterol (FC) as well as cholesteryl ester and appear to have high rates of phospholipid (PL) synthesis and increased PL mass. Previous short term (i.e. </=24 h) studies with cultured macrophages have shown that these cells respond to FC loading by up-regulating phosphatidylcholine biosynthesis. We propose that this response is adaptive by keeping the FC:PL ratio in the macrophages from reaching toxic levels. We further propose that one cause of macrophage necrosis, a prominent and important event in atherosclerosis, is an eventual decrease of this adaptive response. To explore these ideas, cultured macrophages were loaded with FC for up to 4 days and assayed for phosphatidylcholine biosynthesis, FC and PL mass, and cytotoxicity. For the first 24 h, cellular phosphatidylcholine biosynthesis and FC and PL mass increased 3-4-fold, and thus the FC:PL molar ratio was prevented from reaching very high levels; at this point, there were no overt signs of cytotoxicity. Over the next 24-48 h, however, phosphatidylcholine biosynthesis, and then phosphatidylcholine mass, began to decrease. Initially, the macrophages remained healthy and continued to accumulate FC, but eventually these macrophages, but not unloaded macrophages, became necrotic (swollen organelles and disrupted membranes). Lipoprotein dose studies indicated a close relationship between the onset of macrophage necrosis and the FC:PL ratio. To test further the causal nature of these relationships, cellular FC and PL mass were independently manipulated by using high density lipoprotein3 (HDL3) to decrease cellular FC and choline depletion to decrease cellular PC. As predicted by our hypotheses, HDL3 protected FC-loaded macrophages from necrosis, whereas choline depletion accelerated cytotoxic changes. These findings support the idea that the initial increase in phosphatidylcholine biosynthesis in FC-loaded macrophages is an adaptive response that prevents cholesterol-induced macrophage necrosis. We propose that an eventual failure of the PL response in foam cells may represent one cause of macrophage necrosis in advanced atherosclerotic lesions.
authors.affiliation: Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
authors.forename: I
authors.initials: I
authors.lastname: Tabas
authors.forename: S
authors.initials: S
authors.lastname: Marathe
authors.forename: G A
authors.initials: GA
authors.lastname: Keesler
authors.forename: N
authors.initials: N
authors.lastname: Beatini
authors.forename: Y
authors.initials: Y
authors.lastname: Shiratori
mesh_terms: D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology*; D002470:Cell Survival; D002784:Cholesterol / Q000502:physiology*; D005487:Foam Cells / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008264:Macrophages / Q000502:physiology*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009336:Necrosis; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D010713:Phosphatidylcholines; D002784:Cholesterol