title: Crystallization of a mammalian ornithine decarboxylase.
abstract: Crystals of truncated (delta425-461) pyridoxal-5'-phosphate (PLP)-dependent mouse ornithine decarboxylase (mOrnDC') have been obtained that diffract to 2.2 angstroms resolution (P2(1)2(1)2, a = 119.5 angstroms, b = 74.3 angstroms, c = 46.1 angstroms). OrnDC produces putrescine, which is the precursor for the synthesis of polyamines in eukaryotes. Regulation of activity and understanding of the mechanism of action of this enzyme may aid in the development of compounds against cancer. mOrnDC is a member of group IV PLP-dependent decarboxylases, for which there are no known representative structures.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kern
authors.forename: M A
authors.initials: MA
authors.lastname: Oliveira
authors.forename: N L
authors.initials: NL
authors.lastname: Chang
authors.forename: S R
authors.initials: SR
authors.lastname: Ernst
authors.forename: D W
authors.initials: DW
authors.lastname: Carroll
authors.forename: C
authors.initials: C
authors.lastname: Momany
authors.forename: K
authors.initials: K
authors.lastname: Minard
authors.forename: P
authors.initials: P
authors.lastname: Coffino
authors.forename: M L
authors.initials: ML
authors.lastname: Hackert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D000518:Eflornithine / Q000031:analogs & derivatives / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000737:chemistry*; D065108:Ornithine Decarboxylase Inhibitors; D010446:Peptide Fragments / Q000737:chemistry; D011732:Pyridoxal Phosphate / Q000737:chemistry; D017384:Sequence Deletion
chemical_list: D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D010446:Peptide Fragments; D011732:Pyridoxal Phosphate; C037000:2-fluoromethylornithine; D009955:Ornithine Decarboxylase; D000518:Eflornithine