title: Proteases associated with invadopodia, and their role in degradation of extracellular matrix.
abstract: Metastasizing cancer cells invade the extracellular matrix using plasma membrane protrusions (invadopodia) that contact and dissolve the matrix. Evidence suggests that membrane-associated proteases, 170-kD gelatinase (seprase) and Gelatinase A, exert their mechanisms of action on invadopodia. Potential roles that other metallo- and serine-types of membrane proteases, including membrane-type matrix metalloprotease, meprin, dipeptidyl peptidase IV, fibroblast activation protein alpha and guanidinobenzoatase, play in the cell surface proteolysis are also discussed. It is proposed that formation of a structurally and functionally linked protease complex on invadopodia allows the invasion of cancer cells into the extracellular matrix.
authors.affiliation: Department of Cell Biology, Georgetown University School of Medicine, Washington, D.C. 20007, USA.
authors.forename: W T
authors.initials: WT
authors.lastname: Chen
mesh_terms: D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000201:enzymology*; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2; D053504:Matrix Metalloproteinases, Membrane-Associated; D008565:Membrane Proteins / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000473:pathology*; D012697:Serine Endopeptidases / Q000378:metabolism; D008625:Tiopronin / Q000378:metabolism; D000099167:Fibroblast Activation Protein Alpha
chemical_list: D018819:Dipeptidyl Peptidase 4; D010450:Endopeptidases; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D053504:Matrix Metalloproteinases, Membrane-Associated; D008565:Membrane Proteins; D008666:Metalloendopeptidases; D012697:Serine Endopeptidases; D008625:Tiopronin; D000099167:Fibroblast Activation Protein Alpha