Polyclonal anti-idiotypic antibodies to idazoxan and their interaction with human brain imidazoline binding sites.
Polyclonal antibodies were raised in rabbits against purified polyclonal anti-idazoxan antibodies. The anti-idiotypic antibodies thus obtained, proved able to inhibit [3H]idazoxan specific binding to anti-idazoxan antibodies. Applied to human nucleus reticularis lateralis membrane preparations, these antibodies (20 micrograms) inhibited about 50 and 70% of the imidazoline specific binding of [3H]idazoxan and [3H]clonidine, respectively. Furthermore, they specifically immunoprecipitated 50% of [3H]idazoxan binding activity of imidazoline binding sites solubilized from the same tissue. [3H]Rauwolscine binding to alpha 2-adrenoceptors in rat cortex was not significantly affected by these antibodies. The antibodies labeled a 43 kDa protein in Western blots of partially purified imidazoline binding sites from human brain. In conclusion, these anti-idiotypic antibodies recognize imidazoline binding sites from human brain and allow the detection of a 43 kDa binding protein associated with or representing the imidazoline receptor expressed in human brain.
Bennai
F
F
Laboratoire de Pharmacologie Cardiovasculaire et Rénale, CNRS URA 589, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.
Greney
H
H
Vonthron
C
C
Bousquet
P
P
Dontenwill
M
M
D000316:Adrenergic alpha-Agonists / Q000276:immunology / Q000378:metabolism*; D000317:Adrenergic alpha-Antagonists / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity / Q000276:immunology; D001921:Brain / Q000378:metabolism*; D003000:Clonidine / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D019329:Idazoxan / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011955:Receptors, Drug / Q000276:immunology / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D000888:Antibodies, Anti-Idiotypic; D011955:Receptors, Drug; D003000:Clonidine; D019329:Idazoxan