title: Structural and serological studies of lipopolysaccharides of Citrobacter O35 and O38 antigenically related to Salmonella.
abstract: Structural analysis using 13C NMR spectroscopy and methylation showed that lipopolysaccharides (LPSs) of Citrobacter freundii O35 and Salmonella arizonae O59 have structurally identical O-specific polysaccharide chains, and those of C. freundii O38 and Salmonella kentucky differ only in the presence of O-acetyl groups in the former. Serological relationships between the structurally similar LPSs were demonstrated using inhibition of ELISA, rocket immunoelectrophoresis, double gel diffusion, and immunoblotting. The O-acetyl groups present in C. freundii O38 LPS are of little importance for its serological specificity. A cross-reaction was observed in immunoblotting between O-antisera to C. freundii O35 and S. arizonae O59 and a structurally related LPS of Pseudomonas aeruginosa O11a, 11b (Lányi-Bergan classification).
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: E S
authors.initials: ES
authors.lastname: Stanislavsky
authors.forename: E V
authors.initials: EV
authors.lastname: Kholodkova
authors.forename: C
authors.initials: C
authors.lastname: Lugowski
authors.forename: W
authors.initials: W
authors.lastname: Jachymek
authors.forename: E
authors.initials: E
authors.lastname: Romanowska
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D002240:Carbohydrate Sequence; D016971:Citrobacter freundii / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D003429:Cross Reactions; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000145:classification / Q000276:immunology; D012476:Salmonella arizonae / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D013329:Structure-Activity Relationship
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides