title: Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.
abstract: cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2-terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein (approximately 34 kDa) compared to mouse. PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death.
authors.affiliation: Bumham Institute, La Jolla, California 92037, USA.
authors.forename: S
authors.initials: S
authors.lastname: Takayama
authors.forename: K
authors.initials: K
authors.lastname: Kochel
authors.forename: S
authors.initials: S
authors.lastname: Irie
authors.forename: J
authors.initials: J
authors.lastname: Inazawa
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Druck
authors.forename: K
authors.initials: K
authors.lastname: Huebner
authors.forename: J C
authors.initials: JC
authors.lastname: Reed
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006801:Humans; D006822:Hybrid Cells; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012377:Rodentia; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C091732:BCL2-associated athanogene 1 protein; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D014157:Transcription Factors