Autoimmune MRL mice express high-affinity IgG2b monoclonal autoantibodies to heparin.
Heparin and heparan sulfate are related glycosaminoglycans which demonstrate high-affinity interactions with a number of proteins, including antithrombin III. The immunogenicity of heparin has been reported previously employing heparin-protein conjugates as immunogens and as antigens in solid-phase assays. Previous studies also demonstrate that anti-heparin antibodies play a role in autoimmune diseases including systemic lupus and anti-phospholipid syndrome and in patients who receive heparin for therapeutic purposes. In the current study, we investigated the expression of monoclonal anti-heparin antibodies in nonimmunized, autoimmune MRL/lpr/lpr++ mice employing a liquid-phase radioimmunoassay. The Kd of monoclonal IgG2b autoantibodies for heparin was approximately 10(-8)M. Anti-heparin antibodies were precipitating, and were not polyreactive. The IgG monoclonal antibodies described in this study represent an immunological instance of a specific, high-affinity heparin-protein interaction.
Fillit
H
H
The Henry L. Schwartz Department of Geriatrics and Adult Development, The Mount Sinai Medical Center, New York, New York 10029-6574, USA.
Edstrom
W
W
Yang
C P
CP
Moran
T
T
Dimitriu-Bona
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D016736:Antiphospholipid Syndrome / Q000276:immunology; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D005260:Female; D006493:Heparin / Q000276:immunology*; D006497:Heparan Sulfate / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000378:metabolism*; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006493:Heparin; D006497:Heparan Sulfate; D007074:Immunoglobulin G