Applications of laser scanning microscopy for analysis of aquatic microhabitats.
Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages.
Ghiorse
W C
WC
Section of Microbiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853, USA.
Miller
D N
DN
Sandoli
R L
RL
Siering
P L
PL
D000818:Animals; D001345:Autoradiography; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D018441:Biofilms; D017404:In Situ Hybridization, Fluorescence; D008277:Magnesium Oxide / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D015347:RNA Probes / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D011817:Rabbits; D014871:Water Microbiology
D015347:RNA Probes; D012336:RNA, Ribosomal, 16S; D008277:Magnesium Oxide