The breast mucin MUCI as a novel adhesion ligand for endothelial intercellular adhesion molecule 1 in breast cancer.
The MUC 1 mucin is expressed on normal breast epithelium and in 90% of breast cancers. We report here that tumor-associated MUC1 is a ligand for intercellular adhesion molecule 1 (ICAM-1). Antibodies to ICAM-1 and to MUC1 inhibited adhesion of human and transfected mouse MUC1-positive cell lines to human umbilical vein endothelial cell monolayers and immobilized recombinant human ICAM-1-immunoglobulin fusion protein. Purified MUC1 pretreatment of recombinant human ICAM-1 was an equally effective inhibitor of adhesion. The interaction between MUC1 and ICAM-1 may be critical to the process of bloodborne metastases in breast cancer.
Regimbald
L H
LH
Department of Laboratory Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
Pilarski
L M
LM
Longenecker
B M
BM
Reddish
M A
MA
Zimmermann
G
G
Hugh
J C
JC
D000818:Animals; D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000098:blood supply* / Q000473:pathology; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008024:Ligands; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply / Q000473:pathology; D051379:Mice; D018396:Mucin-1 / Q000096:biosynthesis / Q000502:physiology*; D009363:Neoplasm Proteins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D008024:Ligands; D018396:Mucin-1; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1