D-Cycloserine biases enrichment for auxotrophic mutants of Zymomonas mobilis.
Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with mostly methionine mutants isolated. A decrease of the D-cycloserine concentration only reduced the bias.
Pencreac'h
G
G
Université de la Méditerranée, ERS CNRS 157, Département de Chimie, Marseille, France.
Aït-Abdelkader
N
N
Joset
F
F
Baratti
J C
JC
D000963:Antimetabolites / Q000494:pharmacology*; D002470:Cell Survival; D003470:Culture Media; D003523:Cycloserine / Q000494:pharmacology*; D018698:Glutamic Acid / Q000187:drug effects; D005998:Glycine / Q000187:drug effects; D008715:Methionine / Q000235:genetics; D008903:Minerals / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects; D017355:Zymomonas / Q000187:drug effects* / Q000235:genetics*
D000963:Antimetabolites; D003470:Culture Media; D008903:Minerals; D018698:Glutamic Acid; D003523:Cycloserine; D008715:Methionine; D005998:Glycine