No differences in mucosal adaptive growth one week after intestinal resection in rats given enteral glutamine supplementation or deprived of glutamine.
OBJECTIVE
To evaluate the effects of intraluminal glutamine on the adaptation of intestinal mucosa after resection compared with transsection and un-operated on control animals.


METHODS
Open, controlled, experimental study.


METHODS
University hospital, Sweden.


METHODS
123 Sprague-Dawley rats.


METHODS
Daily isonitrogenous oral diet was given either free of glutamine or supplemented with 4% glutamine for 2 or 7 days to rats subjected to intestinal resection, transection or no operation.


METHODS
Body weight and protein content, DNA content, and thymidine incorporation in jejunal and ileal mucosa.


RESULTS
Resection resulted in a significant growth stimulation evaluated by weight/body weight, protein, and DNA content (p < 0.05-0.001). Glutamine supplementation did not significantly influence this growth response. Thymidine incorporation in jejunum was stimulated by glutamine on day 3 (p < 0.05-0.001).


CONCLUSIONS
The glutamine fortified diet had no growth stimulating effects compared with a glutamine free diet one week after 60% intestinal resection. An early increase in thymidine incorporation indicated that glutamine had a transient proliferative effect.
Wirén
M E
ME
Department of Surgery, Faculty of Health Sciences, University of Linköping, Sweden.
Permert
J
J
Skullman
S P
SP
Wang
F
F
Larsson
J
J
D000222:Adaptation, Physiological; D000284:Administration, Oral; D000818:Animals; D001835:Body Weight; D002455:Cell Division; D004247:DNA / Q000378:metabolism; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D007082:Ileum / Q000166:cytology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D007421:Intestine, Small / Q000601:surgery*; D007583:Jejunum / Q000166:cytology / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D005973:Glutamine; D004247:DNA; D013936:Thymidine