title: Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
abstract: A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.
authors.affiliation: Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
authors.forename: N
authors.initials: N
authors.lastname: Baskaran
authors.forename: R P
authors.initials: RP
authors.lastname: Kandpal
authors.forename: A K
authors.initials: AK
authors.lastname: Bhargava
authors.forename: M W
authors.initials: MW
authors.lastname: Glynn
authors.forename: A
authors.initials: A
authors.lastname: Bale
authors.forename: S M
authors.initials: SM
authors.lastname: Weissman
mesh_terms: D001482:Base Composition; D001622:Betaine; D002352:Carrier Proteins / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide; D005260:Female; D005600:Fragile X Syndrome / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D027702:Organic Cation Transporter 1; D016133:Polymerase Chain Reaction / Q000379:methods*; D011990:Receptors, Transferrin / Q000235:genetics; D013698:Templates, Genetic; D018911:Trinucleotide Repeats
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1; D011990:Receptors, Transferrin; D001622:Betaine; D004247:DNA; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide