Thin-film microbiosensors for glucose-lactate monitoring.
A miniaturized device for simultaneous measurement of glucose and lactate levels was produced by means of photopatterning of enzyme-containing photosensitive membrane precursors. This device shows no cross-talk and a lifetime for both the glucose and the lactate sensors of more than 2 weeks when continuously operated in undiluted bovine serum. Linear response ranges of up to 40 mM for glucose and 25 mM for L-lactate, in combination with 95% response times of < 30 s, were realized. The devices are mass produced by means of thin-film technology on flexible carriers to give catheter-type multisensing devices for in vivo applications. Ex vivo experiments, performed with human volunteers, where the device was continuously operated in an extracorporeal, undiluted, heparinized blood stream for 6 h, gave a correlation of r > 0.98 with respect to laboratory techniques. Subcutaneous measurements of glucose levels in pigs were close to the corresponding blood levels obtained without in vivo calibration.
Jobst
G
G
Institut für Allgemeine Elektrotechnik und Elektronik, Vienna University of Technology, Austria.
Moser
I
I
Varahram
M
M
Svasek
P
P
Aschauer
E
E
Trajanoski
Z
Z
Wach
P
P
Kotanko
P
P
Skrabal
F
F
Urban
G
G
D000328:Adult; D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D004563:Electrochemistry; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives; D013552:Swine
D001786:Blood Glucose; C097029:poly(2-hydroxyethyl methacrylate-co-acrylic acid); D011102:Polyhydroxyethyl Methacrylate; D019344:Lactic Acid