Hypersecretion of follicle-stimulating hormone (FSH) on estrous afternoon in rats treated with RU486 in proestrus.
1. Intact or ovariectomized (OVX) cyclic rats injected or not with RU486 (4 mg/0.2 ml oil) from proestrus onwards were bled at 0800 and 1800 h on proestrus, estrus and metestrus. Additional RU486-treated rats were injected with: LHRH antagonist (LHRHa), estradiol benzoate (EB) or bovine follicular fluid (bFF) and sacrificed at 1800 h in estrous afternoon. LH and FSH serum levels were determined by RIA. 2. RU486-treated intact or OVX rats had decreased preovulatory surges of LH and FSH, abolished secondary secretion of FSH and hypersecretion of FSH in estrous afternoon. The latter was decreased by LHRHa and abolished by EB or bFF. In contrast, EB induced an hypersecretion of LH in RU486-treated rats at 1800 h in estrus. 3. It can be concluded that in the absence of the proestrous progesterone actions, the absence of the inhibitory effect of the ovary in estrus evoked a LHRH independent secretion of FSH.
Tébar
M
M
Department of Physiology, Faculty of Medicine, University of Córdoba, Spain.
Ruiz
A
A
Sánchez-Criado
J E
JE
D000818:Animals; D002417:Cattle; D002940:Circadian Rhythm / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D006727:Hormone Antagonists / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism; D008686:Metestrus / Q000187:drug effects; D015735:Mifepristone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000737:chemistry; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011359:Proestrus / Q000187:drug effects; D011374:Progesterone / Q000037:antagonists & inhibitors* / Q000502:physiology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D006727:Hormone Antagonists; D015735:Mifepristone; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone