Rapid, solid phase extraction technique for the high-throughput assay of darifenacin in human plasma.
A novel method has been developed for the rapid solid phase extraction of drugs and metabolites from biological fluids, prior to further analysis. The newly designed, 96-tube micropreparation block facilitates high throughput by enabling the extraction of 96 samples simultaneously. The system is described, linked to HPLC/APCI-MS/MS, for the determination of darifenacin in human plasma. The resulting procedure, using deuterated darifenacin as internal standard, is validated over the concentration range 25-2000 pg/mliter; accuracy (0.6-4.6%) and precision (3.6-18.8%) are considered acceptable and overall recovery was determined to be approximately 50%.
Kaye
B
B
Department of Drug Metabolism, Pfizer Central Research, Sandwich, Kent, U.K.
Herron
W J
WJ
Macrae
P V
PV
Robinson
S
S
Stopher
D A
DA
Venn
R F
RF
Wild
W
W
D001572:Benzofurans / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011759:Pyrrolidines / Q000097:blood*; D012680:Sensitivity and Specificity
D001572:Benzofurans; D011759:Pyrrolidines; C101207:darifenacin