Statistical optimization of a reversed-phase liquid chromatographic method for the determination of amiloride and methyclothiazide in tablets.
A quantitative high-performance liquid chromatographic method in which amiloride is separated from methyclothiazide on a C18 column with detection at 286 mm was developed with the aid of the 'window diagram' technique of Laub and Purnell. The effect of simultaneously varying the pH and methanol to water ratio in the mobile phase were studied to optimize the separation. The method was applied to the quantitative analysis of Lometazid tablets. The powdered tablets were extracted with methanol, containing phenacetin as the internal standard, and assayed by comparison of peak heights after liquid chromatography.
Zivanovic
L J
LJ
Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Belgrade, Serbia, Minor Yugoslavia.
Vasiljevic
M
M
Agatonovic-Kustrin
A
A
Maksimovic
M
M
D000584:Amiloride / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D008736:Methyclothiazide / Q000032:analysis*; D013607:Tablets
D013607:Tablets; D000584:Amiloride; D008736:Methyclothiazide; D000432:Methanol