An anti-CD3 epsilon serum detects T lymphocytes in paraffin-embedded pathological tissues in many animal species.
The in situ identification of T cells has been difficult and restricted to frozen tissue samples of a limited range of species. In this study, we demonstrate that an antiserum recognizing a phylogenetically conserved part of the CD3 epsilon cytoplasmic tail detects T cells of many avian and mammalian species in formalin-fixed paraffin-embedded tissue sections. This antiserum enables the morphological characterization of normal and pathological lymphoid tissues and lymphoid infiltrations in experimental work and in animal disease.
Keresztes
G
G
Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary. immun@everx.szbk.u-szeged.hu
Glávits
R
R
Krenács
L
L
Kurucz
E
E
Andó
I
I
D000818:Animals; D017252:CD3 Complex / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D010232:Paraffin; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; D010232:Paraffin