Structural changes leading to increased enzymatic activity in an engineered variant of Bacillus lentus subtilisin.
Bott
R
R
Genencor International, South San Francisco, CA 94080, USA.
Dauberman
J
J
Wilson
L
L
Ganshaw
G
G
Sagar
H
H
Graycar
T
T
Estell
D
D
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D003196:Computer Graphics; D014644:Genetic Variation; D006860:Hydrogen Bonding; D006868:Hydrolysis; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D012694:Serine / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013381:Subtilisins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
D012694:Serine; D013381:Subtilisins