Enzymatic method for measuring subnanomolar concentrations of oxygen in gaseous or aqueous solutions.
A method that combines glucose oxidase with [U-14C]glucose to measure subnanomolar to decamillimolar concentrations of oxygen is presented. The method is inexpensive and easy to use. It is independent of sample pH and enzyme inhibitors, including detergents and metal ions. This method can be directly applied to gaseous or aqueous samples and multiple assays can be run simultaneously.
O'Fallon
J V
JV
Department of Animal Sciences, Washington State University, Pullman, Washington, 99164-6320, USA.
Wright
R W
RW
Calza
R E
RE
D002238:Carbohydrate Epimerases / Q000378:metabolism; D003902:Detergents; D004791:Enzyme Inhibitors / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008670:Metals; D010100:Oxygen / Q000032:analysis*; D019289:Pyruvic Acid / Q000378:metabolism; D012996:Solutions / Q000737:chemistry*
D003902:Detergents; D004791:Enzyme Inhibitors; D008670:Metals; D012996:Solutions; D019289:Pyruvic Acid; D005949:Glucose Oxidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase; D005947:Glucose; D010100:Oxygen