A novel method for purification of catalytic antibodies toward DNA from sera of patients with lymphoproliferative diseases.
A novel highly efficient method of purification of DNA-hydrolyzing antibodies from sera of patients with lymphoproliferative diseases was developed. The method suggests purification of serum sample using Cibacron Blue 3GA, ion exchange chromatography on DEAE-Sepharose FF, PrG affinity chromatography and selection of the DNA-binding fraction on DNA-Sephacryl. The application of the method allows to obtain the stable and active polyclonal preparation with increased content of DNA-hydrolyzing antibodies characterized by specific binding constant determined using heteropolymeric oligonucleotide substrate. The unusual properties of anti-DNA catalytic antibodies in respect to their interactions with DEAE ion exchanger are discussed.
Kozyr
A V
AV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
D000906:Antibodies / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D004247:DNA / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D007158:Immunologic Techniques; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000276:immunology*
D000906:Antibodies; D004247:DNA