Production of hyaluronan by glomerular mesangial cells in response to fibronectin and platelet-derived growth factor.
The formation of the non-sulphated glycosaminoglycan hyaluronan by cells of the renal glomerulus in diabetes may contribute to altered matrix composition. We describe an increased production of hyaluronan from mesangial cell-enriched glomerular cores from diabetic animals, and further show that increased hyaluronan production follows the exposure of non-diabetic and diabetic preparations to fibronectin and to platelet-derived growth factor in vitro. Hyaluronan production appeared dependent on protein kinase C activity, and could not be shown after prolonged phorbol ester preincubation. Stimulation by fibronectin was wholly dependent on cyclooxygenase activity and prior prostaglandin production, while the effect of platelet-derived growth factor showed only a partial dependence.
Dunlop
M E
ME
Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Victoria, Australia.
Clark
S
S
Mahadevan
P
P
Muggli
E
E
Larkins
R G
RG
D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011493:Protein Kinase C / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D005353:Fibronectins; D010972:Platelet Activating Factor; D006820:Hyaluronic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate