Application of cloth-based enzyme immunoassay for the characterization of monoclonal antibodies to Salmonella lipopolysaccharide antigens.
The specificity, detection limit, and stability of twelve anti-Salmonella monoclonal antibodies (Mabs) were evaluated by cloth-based enzyme immunoassay (CEIA) and polymyxin-cloth based enzyme immunoassay (p-CEIA). Using the p-CEIA, five Mabs were found to react with cholate extracted lipopolysaccharide (LPS) antigens of all 44 Salmonella strains representing 19 different serogroups examined, with the exception of the one strain of serogroup-O tested. These five Mabs did not react with cholate extracts of any of 16 Gram-positive or Gram-negative non-Salmonella bacteria tested. The detection limit of purified S. typhimurium LPS antigen in the p-CEIA was approximately 40 ng for four of the Mabs and approximately 20 ng for the other Mab. Four of the five Mabs were stable during storage at 22 degrees C-23 degrees C for 24 h. These four Mabs are potentially useful for the immunodetection of Salmonella in foods and other samples.
Brown
J G
JG
Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Brooks
B W
BW
Blais
B W
BW
Yamazaki
H
H
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D004355:Drug Stability; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000276:immunology*; D011091:Polyesters; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D011091:Polyesters