title: A combinatorial library strategy for the rapid humanization of anticarcinoma BR96 Fab.
abstract: We have used a combinatorial mutagenesis strategy to humanize BR96, a monoclonal antibody that binds to the Lewis Y class of tumor antigens. This approach allows simultaneous assessment of hundreds of humanized variable regions to identify the molecules that best preserve affinity, thus overcoming the major drawback of current humanization procedures, the requirement to construct and analyze each humanized antibody separately. Murine residues of BR96 were mutated to human if they were solvent-exposed residues that did not participate in the formation of the antigen binding site and were not at the interface of the light and heavy chain. At positions that might be involved in binding to antigen, the choice between the murine and human residue was more difficult. Murine and human alternatives were incorporated into a combinatorial library at positions representing buried residues that might affect the structural integrity of the antigen binding site. By encoding this library of humanized BR96 Fabs in an M13 phage vector, we rapidly identified several candidates with nearly identical antigen binding, within 2-fold, of the chimeric Fab. Additional mutagenesis directed at sites suggested in the literature as potentially important for antigen binding in a similar anti-Lewis Y antibody yielded no further improvements.
authors.affiliation: Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Rosok
authors.forename: D E
authors.initials: DE
authors.lastname: Yelton
authors.forename: L J
authors.initials: LJ
authors.lastname: Harris
authors.forename: J
authors.initials: J
authors.lastname: Bajorath
authors.forename: K E
authors.initials: KE
authors.lastname: Hellström
authors.forename: I
authors.initials: I
authors.lastname: Hellström
authors.forename: G A
authors.initials: GA
authors.lastname: Cruz
authors.forename: K
authors.initials: K
authors.lastname: Kristensson
authors.forename: H
authors.initials: H
authors.lastname: Lin
authors.forename: W D
authors.initials: WD
authors.lastname: Huse
authors.forename: S M
authors.initials: SM
authors.lastname: Glaser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D015195:Drug Design; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region; D007983:Lewis Blood Group Antigens / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013698:Templates, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D011993:Recombinant Fusion Proteins