ITSs typing of P.carinii samples from Italy, The Netherlands and Tanzania.
P.carinii molecular epidemiology appears a new interesting investigational field to understand distribution and incidence of isolates from different geographical locations. Recently a typing system, the Type Specific Oligoblotting (TSO) based on 6 different sequences of the Internal Transcribed Spacers (ITSs) of P.carinii rRNA has been developed [1]. By using P.carinii ITSs nested PCR followed by TSO hybridization we have typed 55 lung derived specimens collected in Italy, The Netherlands and sub-Saharian Africa from pts with microscopically detected P.carinii pneumonia.
Atzori
C
C
II Div. Inf. Dis., L.Sacco Hosp. Milan, Italy.
Agostoni
F
F
Cargnel
A
A
Perenboom
R
R
Beckers
P
P
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology / Q000382:microbiology; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D016533:Mycological Typing Techniques; D009426:Netherlands / Q000453:epidemiology; D011010:Pneumocystis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000453:epidemiology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013636:Tanzania / Q000453:epidemiology
D004271:DNA, Fungal