Calcitonin gene-related peptide level in the rat dentate gyrus increases after damage.
Calcitonin gene related peptide-like immunoreactivity (CGRP-LI) was examined in rat dentate gyrus (DG) following damage to granule cells by adrenalectomy or intrahippocampal colchicine injections. In normal DG, CGRP-LI was present in a diffuse hand within the inner third of the molecular layer (ITML) and in hilar cells. Following the experimental procedures, levels of CGRP-LI increased bilaterally in the ITML and in hilar interneurons resembling mossy cells. Ultrastructural analysis of the ITML revealed that CGRP-LI is associated with large, dense-core vesicles within axon terminals which form asymmetrical synapses with dendritic spines, and within dendritic spines. The increase in CGRP-LI level following granule cell damage suggests a protective role for CGRP in the response to hippocampal injury.
Bulloch
K
K
Laboratory of Neuroendocrinology, Rockefeller University, New York, NY 10021, USA.
Prasad
A
A
Conrad
C D
CD
McEwen
B S
BS
Milner
T A
TA
D000315:Adrenalectomy / Q000009:adverse effects; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D003078:Colchicine; D018891:Dentate Gyrus / Q000187:drug effects / Q000293:injuries / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D007150:Immunohistochemistry; D008297:Male; D008845:Microinjections; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D015740:Calcitonin Gene-Related Peptide; D003078:Colchicine