Characterization of the human myeloid cell nuclear differentiation antigen gene promoter.
MNDA (myeloid cell nuclear differentiation antigen) is an interferon alpha regulated nuclear protein expressed only in cells of the human myelomonocytic lineage. To identify mechanisms responsible for this lineage-specific and interferon-regulated expression, the 5' flanking sequence of the gene has been characterized. Two interferon-stimulated response elements (ISRE) flank a multiple transcription start site region identifying MNDA as a TATA-less interferon-regulated gene. Other DNA elements present include a cluster of Myb sites, several Ets, an Ets related PU.1 site and an Sp1 site located within 600 bp of the transcription start sites. In addition, DNA methylation was revealed as one of the possible factors in establishing MNDA expression. The 5' flanking sequence has promoter activity which is elevated by interferon alpha. The findings indicate that MNDA expression is regulated by mechanisms similar to other myelomonocytic cell specific genes and genes up-regulated by interferon alpha.
Kao
W Y
WY
Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232-5310, USA.
Dworkin
L L
LL
Briggs
J A
JA
Briggs
R C
RC
D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D008745:Methylation; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D015214:Antigens, Differentiation, Myelomonocytic; C075373:MNDA protein, human; D014157:Transcription Factors; D004247:DNA; D007372:Interferons