Crystallization and preliminary X-ray analysis of salicylate hydroxylase from Pseudomonas putida S-1.
Apo-salicylate hydroxylase from Pseudomonas putida S-1 has been crystallized by the dialysis method, using ammonium sulfate as the precipitant. The crystals belong to hexagonal space group P6(2) or P6(4) with unit cell dimensions of a = b = 142.8 A and c = 63.8 A, and diffract X-rays at higher than 3.5 A resolution. A heavy-atom derivative has been prepared by soaking a crystal in an ammonium sulfate solution containing p-chloromercuriphenylsulfonate.
Yabuuchi
T
T
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima.
Suzuki
K
K
Sato
T
T
Ohnishi
K
K
Itagaki
E
E
Morimoto
Y
Y
D003460:Crystallization; D018360:Crystallography, X-Ray; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000302:isolation & purification; D016958:Pseudomonas putida / Q000201:enzymology*
D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase