[Collagen metabolism disturbances with experimentally increased pulmonary flow--experimental model of left to right shunt with congenital heart defects in children].
The studies carried out on young piglets were to demonstrate that experimentally increased pulmonary flow resulted in collagen hyperproduction in the pulmonary tissue. In 14 piglets a modified Blalock-Taussig anastomosis was performed, 9 animals constituted the controls. The survivors included 9 experimental and 8 control piglets. In direct lung biopsies the biochemical collagen content was assessed, whereas histopathology confirmed the development of vascular lesions characteristic for pulmonary hypertension. A significant increase of collagen level in the pulmonary tissue was demonstrated in experimental animals. Determinations were also made of serum and urine hydroxyproline values. A significant increase was observed in serum and urine hydroxyproline values in experimental animals in comparison to the controls when determinations were made 7 days to 3 months after the anastomosis had been performed (p < 0.01). The authors showed that an increase of pulmonary flow in piglets results in collagen metabolism disturbances which are seen both in an increased collagen level in the tissue and in increased serum and hydroxyproline levels.
Skalski
J
J
Z I Kliniki Kardiochirurgii SAM w Katowicach.
Machej
L
L
Radziszewska
E
E
Perlik
T
T
Wites
M
M
Pawlak
S
S
Sleczka
B
B
Bochenek
A
A
Wedrychowska
A
A
D000714:Anastomosis, Surgical; D000818:Animals; D001706:Biopsy; D003094:Collagen / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006330:Heart Defects, Congenital / Q000503:physiopathology; D006909:Hydroxyproline / Q000097:blood / Q000652:urine; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011652:Pulmonary Circulation / Q000502:physiology*; D013552:Swine
D003094:Collagen; D006909:Hydroxyproline