Specific targeting of human papillomavirus type 16 E7 oncogene with triple-helix forming purine oligodeoxyribonucleotides.
Molecular mechanical calculations (computer modelling), optical DNA melting experiments and co-migration assay were used to assess stable helix formation at homopurine-homopyrimidine-rich target sites present in the human papillomavirus type 16 E7 oncogene (positions 656-673 on the genome map). The target sequence, either present in the E7 oncogene obtained by PCR technique or prepared from oligodeoxyribonucleotides (ODNs), can be specifically recognised by different 17-merpurine ODNs designed to form antiparallel or parallel triple helices. These "in vitro" experiments realised with rather long purine ODNs having a high degree of specificity, open the way for "in vivo" tests focused on E7 oncogene targeting and suppression.
Popa
L M
LM
Medical and Pharmaceutical University of Cluj-Napoca, Romania.
Schütz
H
H
Winter
S
S
Kittler
L
L
Löber
G
G
D001483:Base Sequence; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D018390:Gene Targeting / Q000379:methods; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins
D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C051802:triplex DNA; D004247:DNA