Molecular cloning of a cDNA encoding the precursor of Ala-Pro-Gly-Trp amide-related neuropeptides from the bivalve mollusc Mytilus edulis.
We isolated and characterised a cDNA clone encoding the precursor of neuropeptides related to the molluscan neuropeptide Ala-Pro-Gly-Trp amide from the marine mussel Mytilus edulis. The preproprotein is posttranslationally processed to generate a 20 amino acid signal peptide together with five sequences of the neuropeptide Arg-Pro-Gly-Trp amide (RPGWamide), one Lys-Pro-Gly-Trp amide (KPGWamide), one Thr-Pro-Gly-Trp amide (TPGW amide) as well as a putative 31 amino acid long C-terminal peptide. In situ hybridisation showed that the gene encoding this precursor is expressed in discrete neurons within the three ganglia of the central nervous system.
Favrel
P
P
Laboratoire de Biologie et Biotechnologies Marines, Université de Caen, France. favrel@criuc.unicaen.fr
Mathieu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D045683:Furin; D017403:In Situ Hybridization; D007447:Invertebrate Hormones / Q000235:genetics; D008195:Lymnaea; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011498:Protein Precursors / Q000235:genetics; D013381:Subtilisins
D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011498:Protein Precursors; C063193:alanyl-prolyl-glycyl-tryptophanamide; D013381:Subtilisins; D045683:Furin