Subcellular localization of annexin V in human foreskin fibroblasts: nuclear localization depends on growth state.
Annexin V is a major intracellular calcium-binding protein in human foreskin fibroblasts. Immunocytochemistry revealed that annexin V was localized in the nucleus and throughout the cytoplasm in human foreskin fibroblasts. The presence of annexin V in the nucleus was variable depending on the growth state. Nuclear staining was strongest in proliferating cells immediately after sub-culture, and decreased on prolonged culture without changing the culture medium. The cytoplasmic location of annexin V was not greatly affected by the same conditions. Refeeding cells with fresh serum restored annexin V to the nuclei of all cells within 24 h indicating that nuclear localization of annexin V is dependent on serum factors.
Barwise
J L
JL
Department of Biochemistry and Molecular Biology, University of Leeds, UK.
Walker
J H
JH
D017304:Annexin A5 / Q000032:analysis*; D001798:Blood Proteins / Q000494:pharmacology; D015153:Blotting, Western; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry*; D002478:Cells, Cultured; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry; D005347:Fibroblasts; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D012867:Skin / Q000737:chemistry
D017304:Annexin A5; D001798:Blood Proteins; D003470:Culture Media