title: Host-guided migration allows targeted introduction of neurons into the embryonic brain.
abstract: The stereotyped positions occupied by individual classes of neurons are a fundamental characteristic of CNS cytoarchitecture. To study the regulation of neuronal positioning, we injected genetically labeled neural precursors derived from dorsal and ventral mouse forebrain into the telencephalic vesicles of embryonic rats. Cells from both areas were found to participate in the generation of telencephalic, diencephalic, and mesencephalic brain regions. Donor-derived neurons populated the host brain in distinct patterns and acquired phenotypic features appropriate for their final location. These observations indicate that neuronal migration and differentiation are predominantly regulated by non-cell-autonomous signals. Exploiting this phenomenon, intrauterine transplantation allows generation of controlled chimerism in the mammalian brain.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4092, USA.
authors.forename: O
authors.initials: O
authors.lastname: Brüstle
authors.forename: U
authors.initials: U
authors.lastname: Maskos
authors.forename: R D
authors.initials: RD
authors.lastname: McKay
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D002465:Cell Movement; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D014183:Transplantation, Heterologous