An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region.
The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high-resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites, etc. Furthermore, our results have exemplified a strategy for the completion of the chromosome 21 map to sequencing.
Osoegawa
K
K
Institute of Physical and Chemical Research, RIKEN Gene Bank, Tsukuba, Japan.
Susukida
R
R
Okano
S
S
Kudoh
J
J
Minoshima
S
S
Shimizu
N
N
de Jong
P J
PJ
Groet
J
J
Ives
J
J
Lehrach
H
H
Nizetic
D
D
Soeda
E
E
D017101:Bacteriophage P1 / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D016386:Chromosome Walking; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004314:Down Syndrome / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008297:Male; D015183:Restriction Mapping