title: Twelve-well culture plate for the efficient collection and culture of human oocytes and embryos.
abstract: OBJECTIVE
  Our goal was to assess a 12-well oocyte collection and embryo culture plate for use in the IVF laboratory.
  
  
  METHODS
  This was a prospective nonrandomized study.
  
  
  METHODS
  The setting was a university in vitro fertilization program.
  
  
  METHODS
  Eighty-four consecutive infertility couples presenting for IVF were studied.
  
  
  METHODS
  The main outcome measure was pregnancy (delivered).
  
  
  RESULTS
  A 34% delivered pregnancy rate per retrieval was attained using the 12-well collection and culture plate without the use of expensive culture media and special serum supplementation.
  
  
  CONCLUSIONS
  The use of a 12-well plate for oocyte collection and embryo culture provides a simple, economical, efficient, and effective means of producing human embryos during in vitro fertilization. This system is capable of supporting high rates of ongoing and delivered pregnancies.
authors.affiliation: Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville 32610, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Drury
authors.forename: J R
authors.initials: JR
authors.lastname: Konicek
authors.forename: S
authors.initials: S
authors.lastname: Kipersztok
authors.forename: R S
authors.initials: RS
authors.lastname: Williams
mesh_terms: D000328:Adult; D046508:Culture Techniques / Q000295:instrumentation*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D011446:Prospective Studies