title: Verocytotoxin-producing Escherichia coli in children with hemolytic uremic syndrome in the Czech Republic.
abstract: Since October 1988 till July 1995, 35 children (mean age 16 +/- 15.5 months) with classical hemolytic uremic syndrome (HUS) were examined for the presence of verocytotoxin (VT)-producing Escherichia coli (VTEC) infection. Stool samples from all patients were cultured for VTEC strains and tested for free fecal neutralizable VT. Serum samples from 18 patients taken on admission were also tested for antibodies to the lipopolysaccharide (LPS) from E. coli O157, O26, O55, O111, and O128 using the passive hemagglutination assay (PHA). Diagnosis of VTEC infection was established in 28 (80%) patients by the combined use of microbiological and serological techniques. VTEC were isolated from 16 (46%) patients, 5 of them had infection with 2 different VTEC serotypes; 11 (52%) VTEC isolates belonged to the serotypes O26: H11 (5), and O157: H7/NM (6). Free fecal VT was found in 21 (60%) patients. PHA antibodies to one or more LPS were detected in 14 (78%) of 18 patients; 9 had antibodies to O157 LPS and 6 to O26 LPS. We conclude from this study that VTEC are the important cause of pediatric HUS in the Czech Republic and the strains belonging to the serogroups O157 and O26 are the most prevalent.
authors.affiliation: Institute of Medical Microbiology, University Hospital Motol, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Bielaszewska
authors.forename: J
authors.initials: J
authors.lastname: Janda
authors.forename: K
authors.initials: K
authors.lastname: Blahová
authors.forename: J
authors.initials: J
authors.lastname: Feber
authors.forename: V
authors.initials: V
authors.lastname: Potuzník
authors.forename: A
authors.initials: A
authors.lastname: Soucková
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000032:analysis; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003603:Cytotoxins / Q000096:biosynthesis* / Q000276:immunology; D018153:Czech Republic / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000209:etiology* / Q000276:immunology; D005243:Feces / Q000382:microbiology; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008297:Male; D011446:Prospective Studies; D012698:Serologic Tests; D022622:Shiga Toxin 1
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003603:Cytotoxins; D022622:Shiga Toxin 1