title: Anti-endotoxin human monoclonal antibody A6H4C5 (HA-1A) utilizes the VH4.21 gene.
abstract: Human IgM monoclonal antibody A6H4C5 was manufactured by Centocor (Malvern, PA) and used in clinical trials as HA-1A (Centoxin). In vitro, A6H4C6 binds to lipid A and rough-strain, gram-negative bacteria endotoxin. Further analysis of A6H4C5 has shown that it is a polyreactive, cold agglutinin that utilizes the VH4.21 gene segment in germline configuration. It is also a human antibody that binds to human B cells. We have characterized several other independently derived VH4.21 human monoclonal antibodies with the same characteristics as A6H4C5. This group of antibodies may represent a conserved host immune response.
authors.affiliation: Department of Gynecology and Obstetrics, Stanford Medical Center, California 94305, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Bieber
authors.forename: N M
authors.initials: NM
authors.lastname: Bhat
authors.forename: N N
authors.initials: NN
authors.lastname: Teng
mesh_terms: D000373:Agglutinins / Q000276:immunology; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D061067:Antibodies, Monoclonal, Humanized; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology; D003080:Cold Temperature; D003450:Cryoglobulins; D004731:Endotoxins / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology* / Q000378:metabolism; D005803:Genes, Immunoglobulin; D006386:Hemagglutination Tests; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data
chemical_list: D000373:Agglutinins; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D003450:Cryoglobulins; D004731:Endotoxins; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008050:Lipid A; D008070:Lipopolysaccharides; C029443:cold agglutinins; C068069:nebacumab