Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U.S.A. based on the presence of muraminidase-released protein and extracellular factor.
The present study describes the prevalence of muraminidase-released protein (MRP) and extracellular factor (EF) proteins associated with virulence of Streptococcus suis serotype 2 from a collection of USA strains. Sixty-six strains belonging to serotypes 1, 2, 3, 4, 7, and 10, were analyzed with a set of double antibody sandwich ELISAs and Western blots. Nineteen of 34 serotype 2 strains from cases of swine meningitis had the MRP+EF+ phenotype. Five of 7 serotype 2 strains isolated from lungs had an MRP*EF- phenotype. An MRP-EF+ phenotype was found in 4/34 strains isolated from swine meningitis. The MRP*EF- and MRP-EF+ phenotypes have not been reported previously. All strains of serotypes other than 2, including isolates from cases of meningitis, had the MRP-EF- phenotype, suggesting that these strains must have other, as yet undetected, virulence factors.
Galina
L
L
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Vecht
U
U
Wisselink
H J
HJ
Pijoan
C
C
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D010641:Phenotype; D015995:Prevalence; D013290:Streptococcal Infections / Q000453:epidemiology / Q000378:metabolism / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000378:metabolism*; D014481:United States / Q000453:epidemiology
D001426:Bacterial Proteins