title: A PCR-based strategy for the construction of a defined Yersinia enterocolitica O:8 htrA mutant.
authors.affiliation: Department of Medical Microbiology, St. Bartholomew's Hospital, London, UK.
authors.forename: B W
authors.initials: BW
authors.lastname: Wren
authors.forename: A L
authors.initials: AL
authors.lastname: Olsen
authors.forename: R
authors.initials: R
authors.lastname: Stabler
authors.forename: S R
authors.initials: SR
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D033903:Periplasmic Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D012703:Serotyping; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D017931:DNA Primers; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; C061682:DegP protease; D012697:Serine Endopeptidases