title: Rapid in situ harvesting and cytogenetic analysis of perinatal tissue samples.
abstract: Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low O2 conditions, pre-harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8.7 days (range 2-17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis.
authors.affiliation: Wessex Regional Genetics Laboratory, Salisbury District Hospital, Wiltshire, U.K.
authors.forename: A M
authors.initials: AM
authors.lastname: Fisher
authors.forename: A E
authors.initials: AE
authors.lastname: Cockwell
authors.forename: K J
authors.initials: KJ
authors.lastname: Moore
authors.forename: N M
authors.initials: NM
authors.lastname: Gregson
authors.forename: P L
authors.initials: PL
authors.lastname: Campbell
authors.forename: C M
authors.initials: CM
authors.lastname: Campbell
authors.forename: A
authors.initials: A
authors.lastname: Herbert
authors.forename: J C
authors.initials: JC
authors.lastname: Barber
authors.forename: J A
authors.initials: JA
authors.lastname: Crolla
mesh_terms: D000650:Amnion / Q000473:pathology; D001706:Biopsy; D002871:Chromosome Banding / Q000379:methods*; D017364:Collagenases / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D016591:Histocytological Preparation Techniques; D006801:Humans; D009132:Muscles / Q000196:embryology / Q000473:pathology; D010053:Ovary / Q000196:embryology / Q000473:pathology; D010865:Pilot Projects; D010920:Placenta / Q000473:pathology; D011247:Pregnancy; D012867:Skin / Q000196:embryology / Q000473:pathology; D013997:Time Factors; D014470:Umbilical Cord / Q000473:pathology
chemical_list: D017364:Collagenases