title: Site-directed mutagenesis using a uracil-containing phagemid template.
authors.affiliation: Department of Pediatrics, Humboldt University, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Hagemeier
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011755:Pyrophosphatases / Q000172:deficiency; D012641:Selection, Genetic; D013698:Templates, Genetic; D014498:Uracil
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D014498:Uracil; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase