Effect of temperature and viscosity of sieving medium on electrophoretic behavior of sodium dodecyl sulfate-proteins on capillary electrophoresis in presence of pullulan.
Pullulan was used as a sieving medium in high-performance capillary gel electrophoresis to study the effect of temperature and viscosity on electrophoretic behavior of sodium dodecyl sulfate (SDS)-proteins with molecular masses in the range of 14,400-116,000 Da. The migration time decreased with increasing capillary temperature. Equations were derived relating mobility of SDS-proteins to capillary temperature and viscosity of pullulan solution. Linear relationships were obtained from experiments between logarithm of mobility and reciprocal temperature and between double logarithmic values of mobility and viscosity of pullulan solution. The experimental results agree well with the equations.
Nakatani
M
M
Pharmaceutical Research Department, Nippon Boehringer Ingelheim Co., Ltd., Hyogo, Japan.
Shibukawa
A
A
Nakagawa
T
T
D019075:Electrophoresis, Capillary / Q000379:methods*; D005936:Glucans; D007202:Indicators and Reagents; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity
D005936:Glucans; D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions; D012967:Sodium Dodecyl Sulfate; C009109:pullulan