Nitric oxide production during adjuvant-induced and collagen-induced arthritis.
OBJECTIVE
To investigate the role of nitric oxide (NO) production and NO synthase (NOS) induction during adjuvant-induced arthritis (AIA) and collagen-induced arthritis (CIA) in Dark Agouti rats.


METHODS
Urinary nitrate excretion and immune NOS (INOS) messenger RNA (mRNA) expression were measured in the joint, lymph node, spleen, and liver tissues following the induction of either AIA or CIA.


RESULTS
Urinary nitrate excretion and iNOS mRNA expression increased substantially during joint inflammation in both models of arthritis. However, the increases in urinary nitrate excretion and iNOS mRNA expression observed in the joint, liver, and spleen tissues during AIA were greater than those observed during CIA, although iNOS induction in the lymph nodes was similar for both models. A prior injection with Mycobacterium bovis heat-shock protein resulted in suppression of arthritis and NO production in AIA, but not in CIA.


CONCLUSIONS
Differences in NO production during AIA versus CIA are a reflection of the fundamental pathophysiologic differences between these 2 models of arthritis. Thus, NO production in these 2 models could not be merely a nonspecific reaction to the adjuvant injection, nor simply a byproduct of local inflammation in the joint.
Cannon
G W
GW
VA Medical Center, Salt Lake City, UT 84148, USA.
Openshaw
S J
SJ
Hibbs
J B
JB
Hoidal
J R
JR
Huecksteadt
T P
TP
Griffiths
M M
MM
D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism*; D001426:Bacterial Proteins; D003094:Collagen; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology; D006360:Heat-Shock Proteins; D007596:Joints / Q000201:enzymology / Q000276:immunology; D008198:Lymph Nodes / Q000201:enzymology / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D009566:Nitrates / Q000652:urine; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000196:embryology / Q000201:enzymology
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D009566:Nitrates; D012333:RNA, Messenger; D009569:Nitric Oxide; D003094:Collagen; D019001:Nitric Oxide Synthase