Differential expression of 70-kDa heat shock-protein in human oral tumorigenesis.
To understand the biological events underlying the multistep process of oral tumorigenesis we have studied the expression of 70-kDa heat-shock protein, HSP70, in human normal, pre-malignant and malignant oral tissues. Expression of HSP70 was assessed in oral-tissue specimens using a mouse monoclonal antibody against HSP70 by immunostaining and immunoblotting analysis. Strong nuclear and cytoplasmic HSP70 immunostaining was observed in oral squamous-cell carcinomas (30/38). Mild to moderate HSP70 expression was observed in oral dysplastic lesions (19/30) and basal low level of HSP70 was observed in normal oral tissues. The results were corroborated by immunoblotting analysis. The wide variation in HSP70 expression in normal, pre-malignant and malignant oral lesions suggests that it is differentially expressed during oral carcinogenesis and may be implicated in tumor development.
Kaur
J
J
Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Ralhan
R
R
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins