X-ray diffraction pattern from internal structure of bovine rhodopsin.
Pure bovine rhodopsin pellets were prepared by removal of cholic acid from rhodopsin-cholic acid complex, and X-ray diffraction patterns from the internal structure of rhodopsin were obtained for the first time using wet and dry pellet samples. The peaks at around 10 and 4.3 A spacings observed for both samples can be attributed to alpha-helices in rhodopsin molecule, providing direct evidence of the helical structure of rhodopsin. A shoulder peak at around 34 A spacing was also observed for the dry pellet, which can be explained by the first-neighbor distance between rhodopsin molecules in the sample.
Kawaguchi
T
T
Department of Electrical and Computer Engineering, Nagoya Institute of Technology.
Hamanaka
T
T
Kito
Y
Y
D000818:Animals; D002417:Cattle; D019826:Cholic Acid; D002793:Cholic Acids; D011487:Protein Conformation; D012243:Rhodopsin / Q000737:chemistry*; D014961:X-Ray Diffraction
D002793:Cholic Acids; D012243:Rhodopsin; D019826:Cholic Acid