Vitamin E inhibits proliferation of human Tenon's capsule fibroblasts in vitro.
Failure of glaucoma surgery is mostly due to fibrocellular scar formation, derived from Tenon's capsule fibroblasts. In high-risk cases, postoperative Tenon's capsule fibroblast proliferation is inhibited by mitomycin C or 5-fluorouracil. Toxicity to other ocular cell types and the risk of ocular hypotony limits the use of these agents. We have found that d-alpha-tocopherol (vitamin E) was able to inhibit proliferation of in vitro human Tenon's capsule fibroblasts obtained from seven different donors. At 48 h, inhibition of cell proliferation was 30-78% (mean 60%) for 50 microM d-alpha-tocopherol and 46-97% (mean 77%) for 100 microM d-alpha-tocopherol. This inhibition was statistically significant. No cytotoxic effects were observed.
Haas
A L
AL
University Eye Clinic, Inselspital, Bern, Switzerland.
Boscoboinik
D
D
Mojon
D S
DS
Böhnke
M
M
Azzi
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects*; D003239:Connective Tissue Cells; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D014130:Trabeculectomy; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E