Stimulated monocytes release factor XIII subunit A and fibronectin in vitro.
Factor XIII catalyses the formation of covalent bonds between several proteins involved in wound healing. Subunit A of factor XIII has been found inside and on the surface of monocytes and macrophages. In our study we investigated the ability of cultured monocytes to release factor XIII A subunit and its substrate-fibronectin. Human monocytes were cultured for up to 36 hours with lipopolysaccharide (LPS) from Escherichia coli. In supernatants of LPS stimulated monocytes we found 22 +/- 4 nM/ml and 40 +/- 6 nM/ml of factor XIII subunit A after 24 and 36 hours of incubation respectively. We also observed an increase of fibronectin concentration in supernatants of stimulated monocytes. The obtained results suggest that activated monocytes might be a source of factor XIII and fibronectin, which are critical for tissue repair processes.
Kłoczko
J
J
Department of Haematology, Medical Academy of Białystok.
Giedrojć
J
J
Radziwon
P
P
Galar
M
M
Bielawiec
M
M
D002478:Cells, Cultured; D004586:Electrophoresis; D005176:Factor XIII / Q000737:chemistry / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D014945:Wound Healing / Q000502:physiology
D005353:Fibronectins; D008070:Lipopolysaccharides; D005176:Factor XIII