Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1.
The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.
Ayyagari
R
R
National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nestorowicz
A
A
Li
Y
Y
Chandrasekharappa
S
S
Chinault
C
C
van Tuinen
P
P
Smith
R J
RJ
Hejtmancik
J F
JF
Permutt
M A
MA
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006946:Hyperinsulinism / Q000235:genetics*; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers