A new dietary model to study colorectal carcinogenesis: experimental design, food preparation, and experimental findings.
Experimental dietary studies of human colorectal carcinogenesis are usually based on the AIN-76A diet, which is dissimilar to human food in source, preparation, and content. The aims of this study were to examine the feasibility of preparing and feeding rats the diet of a specific human population at risk for colorectal neoplasia and to determine whether changes in the colonic morphology and metabolic contents would differ from those resulting from a standard rat diet. The mean daily food intake composition of a previously evaluated adenoma patient case-control study was used for the "human adenoma" (HA) experimental diet. Foods were prepared as for usual human consumption and processed by dehydration to the physical characteristics of an animal diet. Sixty-four female Sprague-Dawley rats were randomized and fed ad libitum the HA or the AIN-76A diet. Every eight weeks, eight rats from each group were sacrificed, and the colons and contents were examined. Analysis of the prepared food showed no significant deleterious changes; food intake and weight gain were similar in both groups. Compared with the controls, the colonic contents of rats fed the HA diet contained significantly less calcium, concentrations of neutral sterols, total lipids, and cholic and deoxycholic acids were increased, and there were no colonic histological changes other than significant epithelial hyperproliferation. This initial study demonstrated that the HA diet can be successfully processed for feeding to experimental animals and is acceptable and adequate for growth but induces significant metabolic and hyperproliferative changes in the rat colon. This dietary model may be useful for studies of human food, narrowing the gap between animal experimentation and human nutritional research.
Rozen
P
P
Gastroenterology Department, Tel Aviv University, Israel.
Liberman
V
V
Lubin
F
F
Angel
S
S
Owen
R
R
Trostler
N
N
Shkolnik
T
T
Kritchevsky
D
D
D000236:Adenoma; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D003840:Deoxycholic Acid / Q000378:metabolism; D004032:Diet; D004195:Disease Models, Animal; D004848:Epithelium / Q000473:pathology; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
D001647:Bile Acids and Salts; D005227:Fatty Acids; D003840:Deoxycholic Acid; D002118:Calcium