Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta).
An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heme, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB-pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.
Ramana
K V
KV
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kohli
K K
KK
D000633:Aminopyrine N-Demethylase / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D015786:Cytochromes b5 / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D006418:Heme / Q000032:analysis / Q000096:biosynthesis; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008253:Macaca mulatta; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000187:drug effects; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology*
D000927:Anticonvulsants; D006993:Hypnotics and Sedatives; D006418:Heme; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D009245:NADH Dehydrogenase; D010634:Phenobarbital