title: Differential action of branching enzymes according to its source.
abstract: Different branching enzymes were partially purified in order to get some insight into their mechanism of action. Each branching enzyme is able to synthesize in vitro at any moment, even during very short incubation times, only one type of alpha 1,4-alpha 1,6 glucopolysaccharide, which possesses unique and specific properties (lambda max; A; R;% of beta-amylolysis;% alpha 1,6). Between two branching points, each branching enzyme will leave, in average, a constant and specific number of glucoses alpha 1,4-linked, which is characteristic of that particular enzyme. Consequently the structure of the polysaccharide will keep the same independently from the moment of synthesis.
authors.affiliation: Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Argentina.
authors.forename: L R
authors.initials: LR
authors.lastname: Lerner
authors.forename: C R
authors.initials: CR
authors.lastname: Krisman
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002236:Carbohydrate Conformation; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis / Q000737:chemistry; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D011134:Polysaccharides / Q000096:biosynthesis / Q000737:chemistry; D051381:Rats; D011198:Solanum tuberosum / Q000201:enzymology; D013053:Spectrophotometry; D003313:Zea mays / Q000201:enzymology
chemical_list: D005936:Glucans; D011134:Polysaccharides; D006003:Glycogen; D015061:1,4-alpha-Glucan Branching Enzyme; D005947:Glucose