Effect of chloral hydrate on in vivo KCl-induced striatal dopamine release in the rat.
The release of striatal dopamine (DA) and its metabolites in response to locally-induced K+ depolarization was investigated in vivo in chloral hydrate-anesthetized and freely moving rats. KCl at concentrations of 30, 50, and 100 mM induced significant dose-dependent increases in extracellular DA overflow in both chloral hydrate-anesthetized and freely moving rats (P < 0.05). Extracellular levels of dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA) were decreased. The DA overflow in response to 30 mM KCl stimulation in anesthetized rats was significantly greater than that in freely moving rats (P < 0.05). In addition, chloral hydrate anesthesia resulted in a significant decrease in extracellular levels of DOPAC and significant increases in extracellular levels of HVA and 5-HIAA in comparison with freely moving rats (P < 0.05). Furthermore, the basal level of extracellular HVA in chloral hydrate-anesthetized rats was significantly higher than that in freely moving rats. These results suggest that chloral hydrate anesthesia could have significant effects on the pharmacological response of the striatal dopaminergic neurons.
Chen
H T
HT
Department of Radiation, Pathophysiology, and Toxicology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5603, USA.
Kandasamy
S B
SB
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001481:Basal Metabolism; D002697:Chloral Hydrate / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018686:Anesthetics, Intravenous; D015102:3,4-Dihydroxyphenylacetic Acid; D002697:Chloral Hydrate; D006897:Hydroxyindoleacetic Acid; D011189:Potassium Chloride; D004298:Dopamine