Dynamic organization of pre-mRNA splicing factors.
Studies from several laboratories during the past few years have increased our understanding towards the dynamic organization of pre-mRNA splicing factors in the mammalian cell nucleus. Many well characterized splicing factors have been localized in a speckled pattern in the cell nucleus. Upon the activation of RNA polymerase II transcription, splicing factors are recruited to the sites of transcription from sites of reassembly and/or storage. Nascent intron-containing RNA transcripts are spliced at the sites of transcription. The speckled distribution of splicing factors in the nucleus is altered when either transcription or pre-mRNA splicing activities are interrupted suggesting that the organization of the splicing machinery in the interphase nucleus is a direct reflection of the transcriptional activity of the cell.
Huang
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Cold Spring Harbor Laboratory, New York 11724, USA.
Spector
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D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D006801:Humans; D009687:Nuclear Proteins / Q000032:analysis*; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing / Q000502:physiology*; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D012322:RNA Precursors