title: [Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis].
abstract: Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants.
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Cs.Exactas y Naturales, UBA, Buenos Aires, Argentina.
authors.forename: T M
authors.initials: TM
authors.lastname: Alconada
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
mesh_terms: D002473:Cell Wall / Q000187:drug effects*; D002480:Cellulase / Q000494:pharmacology*; D018552:Daucus carota / Q000187:drug effects; D005110:Extracellular Space / Q000201:enzymology; D005638:Fruit / Q000166:cytology* / Q000187:drug effects; D005656:Fungal Proteins / Q000494:pharmacology*; D005670:Fusarium / Q000201:enzymology*; D006603:Hexuronic Acids / Q000032:analysis; D006868:Hydrolysis; D011096:Polygalacturonase / Q000494:pharmacology*; D011523:Protoplasts; D011198:Solanum tuberosum / Q000187:drug effects; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D006603:Hexuronic Acids; C007819:galacturonic acid; D014995:Xylosidases; D011096:Polygalacturonase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase