title: Hyperleptinemia: an early sign of juvenile obesity. Relations to body fat depots and insulin concentrations.
abstract: Leptin, the OB gene product, is an adipocyte-derived circulating protein. In several rodent models of obesity, such as the db/db mice, fa/fa rats, and ventromedial hypothalamus-lesioned mice, as well as adult obese subjects, leptin mRNA expression and the circulating levels are elevated, suggesting resistance to its action. However, it is unknown whether the rise in leptin concentration occurs early in the natural evolution of human obesity or is a chronic adaptation to the obese state. Moreover, whether the distribution of body fat (i.e., visceral vs. subcutaneous abdominal fat) influences circulating leptin levels has not been assessed. We have determined in a group of obese and nonobese children and young adults whether leptin levels 1) are increased early in the development of obesity, 2) are related to a specific fat depot measured by magnetic resonance imaging, 3) vary during hyperinsulinemic, euglycemic, and hyperglycemic clamp studies, and 4) are different in males vs. females. In the basal state, leptin levels were elevated in obese children. Children and adults demonstrated a strong positive correlation between leptin concentrations and the subcutaneous fat depot (r = 0.84, P < 0.001). Surprisingly, a weaker correlation was found with visceral fat mass (r = 0.59, P = 0.001). Leptin levels remained unchanged under both euglycemic and hyperglycemic hyperinsulinemic conditions in both obese and nonobese subjects. A pronounced effect of gender on leptin levels was also observed. We conclude that, early in the development of juvenile obesity, leptin concentrations are elevated and are more closely linked to subcutaneous than visceral fat mass. Acute increases in insulin concentrations do not affect circulating leptin levels.
authors.affiliation: Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: S
authors.initials: S
authors.lastname: Caprio
authors.forename: W V
authors.initials: WV
authors.lastname: Tamborlane
authors.forename: D
authors.initials: D
authors.lastname: Silver
authors.forename: C
authors.initials: C
authors.lastname: Robinson
authors.forename: R
authors.initials: R
authors.lastname: Leibel
authors.forename: S
authors.initials: S
authors.lastname: McCarthy
authors.forename: A
authors.initials: A
authors.lastname: Grozman
authors.forename: A
authors.initials: A
authors.lastname: Belous
authors.forename: D
authors.initials: D
authors.lastname: Maggs
authors.forename: R S
authors.initials: RS
authors.lastname: Sherwin
mesh_terms: D000273:Adipose Tissue / Q000000981:diagnostic imaging / Q000503:physiopathology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D015415:Biomarkers; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood*; D020738:Leptin; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D009765:Obesity / Q000097:blood* / Q000503:physiopathology; D011379:Prognosis; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D011859:Radiography; D051381:Rats
chemical_list: D015415:Biomarkers; D007328:Insulin; D020738:Leptin; D011506:Proteins