title: Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: its possible role in the regulation of angiogenic processes.
abstract: In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5, 9, 14 days of incubation. 48 h after injection glycosaminoglycan (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130:257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.
authors.affiliation: Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ribatti
authors.forename: P
authors.initials: P
authors.lastname: Locci
authors.forename: L
authors.initials: L
authors.lastname: Marinucci
authors.forename: C
authors.initials: C
authors.lastname: Lilli
authors.forename: L
authors.initials: L
authors.lastname: Roncali
authors.forename: E
authors.initials: E
authors.lastname: Becchetti
mesh_terms: D000482:Allantois / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000145:classification / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*
chemical_list: D006025:Glycosaminoglycans; D006493:Heparin; D005944:Glucosamine