Isolation and mapping of a novel human gene encoding a protein containing zinc-finger structures.
We have isolated and characterized a cDNA clone, termed OTK18, representing a novel human gene. The 3754 nucleotides of this clone contain an open reading frame of 2133 nucleotides. As the predicted 711-amino-acid protein contains 13 contiguous zinc-finger stuctures of the C2H2 type, it would be expected to function as a DNA-binding multi-finger protein. The 4.3-kb transcript was expressed in all adult human tissues examined by Northern analysis. The gene was assigned to chromosomal band 19q13.4 by fluorescence in situ hybridization.
Saito
H
H
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Fujiwara
T
T
Takahashi
E I
EI
Shin
S
S
Okui
K
K
Nakamura
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011506:Proteins; D014157:Transcription Factors; C104178:ZNF175 protein, human