title: Effect of pyridone carboxylic acid anti-microbials on the generation of reactive oxygen species in vitro.
abstract: The effects of ofloxacin, ciprofloxacin and balofloxacin on the reactive oxygen species (ROS) levels generated by human neutrophils was examined in vitro; ROS generated in a cell-free, xanthine-xanthine oxidase system was also assessed. The species investigated were superoxide radical anion (O2-), hydrogen peroxide (H2O2) and hydroxyl radical (OH*). Both ofloxacin and ciprofloxacin markedly decreased the levels of O2-, H2O2 and OH* generated by human neutrophils. On the other hand, these drugs did not affect any of the ROS examined in the xanthine-xanthine oxidase system. Balofloxacin showed no significant effect on ROS generated by either system. The present study indicates that ofloxacin and ciprofloxacin may exert an anti-inflammatory action by reducing the potent ROS species excessively generated by neutrophils at the sites of inflammation.
authors.affiliation: Department of Dermatology, Kansai Medical University, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Akamatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Niwa
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Matoba
authors.forename: Y
authors.initials: Y
authors.lastname: Asada
authors.forename: T
authors.initials: T
authors.lastname: Horio
mesh_terms: D000890:Anti-Infective Agents; D002939:Ciprofloxacin / Q000494:pharmacology*; D024841:Fluoroquinolones; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects; D015242:Ofloxacin / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D005609:Free Radicals; D015363:Quinolones; D017382:Reactive Oxygen Species; D002939:Ciprofloxacin; D015242:Ofloxacin; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; C077155:balofloxacin