[Correlation of ECP levels in sputum and blood with parameters of airflow obstruction and hyperreactivity in patients with irreversible bronchial obstruction due to chronic bronchitis].
The aim of this study was to find if there was any correlation between the serum and sputum eosinophil cationic protein (ECP) concentrations with the parameters of airflow obstruction as well as degree of bronchial hyperreactivity. 18 patients aged 40-75 years with irreversible bronchial obstruction (FEV1 > 0.61 or < 70% of the predicted value, FEV1%VC < 60%) without eosinophilia and high hyperreactivity (PC20 0.02 mg/ml -0.4 mg/ml) were examined. The FEV1 reversibility after 400 micrograms MDI salbutamol was 5-10%. ECP was measured by radioimmunosorbent assay using the ECP RIA kit (Pharmacia). The range of the standard curve was 2-100 micrograms ECP/L, while all assays were run in duplicate. The bronchial challenge was performed with histamine. The challenge was stopped when the FEV1 had decreased by > 20%. Serum ECP concentrations ranged from 0.0001-0.063 mg/ml, whereas sputum ECP concentrations were between 0.0002-1.84 mg per g sputum. There was no correlation between sputum and serum ECP concentrations and the degree of hyperreactivity and were not related to the parameters of hyperreactivity and were not related to the parameters of airway obstruction.
Chazan
R
R
Klinika Pneumonologii Akademii Medycznej w Warszawie.
Jaworski
A
A
Grubek-Jaworska
H
H
Droszcz
W
W
D000368:Aged; D000402:Airway Obstruction / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D001798:Blood Proteins / Q000032:analysis*; D016535:Bronchial Hyperreactivity / Q000150:complications / Q000378:metabolism*; D001991:Bronchitis / Q000150:complications / Q000175:diagnosis / Q000378:metabolism*; D002908:Chronic Disease; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D008875:Middle Aged; D012260:Ribonucleases; D013183:Sputum / Q000737:chemistry*
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases