title: Small-volume resuscitation using hypertonic saline improves organ perfusion in burned rats.
abstract: Resuscitation using small volumes (3-5 mL/kg) of 7.5% hypertonic saline (HTS) is effective for hemorrhagic shock. Whether HTS is beneficial for the initial resuscitation of burn injury is not clear. We compared the hemodynamic effects of HTS versus lactated Ringer's solution (LR) and examined organ tissue perfusion during burn resuscitation (R). Full thickness scald burn (35% of total body surface area) was induced in pentobarbital-anesthetized rats. Regional blood flows were measured using radioactive microspheres before and 30 min after burn, and after R with either HTS (4 mL/kg) or LR (at a dose required for equivalent restoration of arterial blood pressure). Data from the HTS-or LR-resuscitated groups were compared to those from a nonresuscitated group (n = 10 in each group). Mean arterial pressure decreased 30% after burn (from 120 +/- 4 to 84 +/- 5 mm Hg, mean +/- SEM) and returned toward baseline (112 +/- 7 mm Hg) at 10 min after R with HTS (4 mL/kg) or LR (22.6 +/- 0.7 mL/kg), but subsequently decreased to 100 +/- 7 mm Hg with HTS and 105 +/- 5 mm Hg with LR at 30 min. In contrast to LR, resuscitation using HTS was associated with tachycardia. Blood flows to the skin and muscle of the normal or burn regions did not change after fluid resuscitation as compared to a nonresuscitated group. Fluid resuscitation transiently increased intestinal perfusion. Similar improvements in blood flow to the spleen were observed with HTS and LR at 10 min after R (from 128 +/- 10 to 156 +/- 15 and from 113 +/- 10 to 145 +/- 26 mL.min-1 x 100 g-1, respectively). However, at 30 min after R, splenic perfusion in the LR group was not different from that in the nonresuscitated group. Blood flows to the brain and kidney increased 39% and 42%, respectively, with HTS. HTS was also associated with pronounced improvements in blood flows to the heart (from 346 +/- 20 to 631 +/- 37 mL.min-1 x 100 g-1), liver (from 36 +/- 2 to 62 +/- 4 mL.min-1 x 100 g-1), and testis (from 29 +/- 2 to 43 +/- 2 mL.min-1 x 100g-1). Resuscitation using HTS was associated with rapid improvement in organ tissue perfusion in anesthetized rats subjected to burn injury. In comparison to LR, greater increases in blood flows to the heart, kidney, liver, and testis were observed with HTS. The results suggest that significant improvement in blood flow distribution can be achieved using HTS at less than one fifth the volume of LR for the initial treatment of burn shock.
authors.affiliation: Department of Anesthesiology, School of Medicine, University of California, Davis 95616, USA.
authors.forename: N D
authors.initials: ND
authors.lastname: Kien
authors.forename: J F
authors.initials: JF
authors.lastname: Antognini
authors.forename: D A
authors.initials: DA
authors.lastname: Reilly
authors.forename: P G
authors.initials: PG
authors.lastname: Moore
mesh_terms: D000818:Animals; D001775:Blood Circulation; D001794:Blood Pressure; D001830:Body Surface Area; D002056:Burns / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D002560:Cerebrovascular Circulation; D003326:Coronary Circulation; D005440:Fluid Therapy / Q000379:methods*; D007422:Intestines / Q000098:blood supply; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000627:therapeutic use; D008102:Liver Circulation; D008297:Male; D008863:Microspheres; D018482:Muscle, Skeletal / Q000098:blood supply; D011868:Radioisotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D015532:Rehydration Solutions / Q000008:administration & dosage / Q000627:therapeutic use*; D012079:Renal Circulation; D012151:Resuscitation; D000077325:Ringer's Lactate; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage / Q000627:therapeutic use*; D012771:Shock, Hemorrhagic / Q000628:therapy; D012867:Skin / Q000098:blood supply; D013154:Spleen / Q000098:blood supply; D013610:Tachycardia / Q000209:etiology; D013737:Testis / Q000098:blood supply
chemical_list: D007552:Isotonic Solutions; D011868:Radioisotopes; D015532:Rehydration Solutions; D000077325:Ringer's Lactate; D012462:Saline Solution, Hypertonic