title: Determination of the pharmaceutical peptide TP9201 by post-column reaction with copper(II) followed by electrochemical detection.
abstract: An electrochemical detection method was applied to the determination of the synthetic peptide TP9201 (Telios Pharmaceuticals). The method utilizes reversed-phase HPLC, followed by post-column formation of Cu(II)-peptide complexes to render peptides electrochemically active via the Cu(III/II) couple. TP9201 is cyclic and N-amidated; the lack of a free amine precludes the use of typical fluorescent labeling reagents. Neither the cyclic structure nor the N-amidation prevented the copper complexation reaction, however. The detection limit in bovine serum was 20 nM, limited by interfering sample peaks, and the detector response was linear in a range 10-400 nM.
authors.affiliation: Chevron Science Center, University of Pittsburgh, PA 15260, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Woltman
authors.forename: J G
authors.initials: JG
authors.lastname: Chen
authors.forename: S G
authors.initials: SG
authors.lastname: Weber
authors.forename: J O
authors.initials: JO
authors.lastname: Tolley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001737:Biuret; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D004563:Electrochemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D010456:Peptides, Cyclic; D003300:Copper; C085362:TP 9201; D001737:Biuret