Identification of a functionally important population in phenol-digesting activated sludge with antisera raised against isolated bacterial strains.
Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge during a period of adaptation to phenol, only an antiserum against one of the isolates, Alcaligenes sp. E2, reacted with the activated sludge after the adaptation period. A kinetic pattern of phenol-oxygenating activity of the activated sludge after the adaptation period was similar to that of strain E2. These results suggest that a functionally important population in the phenol-digesting activated sludge was serologically identified.
Watanabe
K
K
Fundamental Research Laboratories, Tonen Corporation, Saitama, Japan. LDJ03665@niftyserve.or.jp
Hino
S
S
D000818:Animals; D000907:Antibodies, Bacterial; D001673:Biodegradation, Environmental; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006088:Gram-Negative Aerobic Bacteria / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D010578:Petroleum; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D011817:Rabbits; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D000907:Antibodies, Bacterial; D010578:Petroleum; D010636:Phenols; D012722:Sewage; D019800:Phenol