Gene-marking and haemopoietic stem-cell transplantation.
Gene transfer has allowed a number of biological issues in haematopoietic stem-cell transplantation to be addressed. Gene-marking studies have shown that residual malignant cells in infused marrow may contribute to relapse in acute myeloid leukaemia, neuroblastoma and chronic myeloid leukaemia. Double gene-marking techniques with distinguishable retroviral vectors are being used to compare purging techniques and the reconstitution of different sources of stem cells. In allogeneic bone-marrow transplantation, gene-marking has demonstrated that adoptively transferred cytotoxic T cells can persist and reconstitute antiviral immunity.
Heslop
H E
HE
Division of Bone Marrow Transplantation, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Rooney
C M
CM
Brenner
M K
MK
D019264:Adoptive Transfer; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000517:prevention & control* / Q000821:virology; D008991:Monitoring, Physiologic / Q000379:methods; D012307:Risk Factors; D014184:Transplantation, Homologous
D005819:Genetic Markers