title: Genomic sequencing by ligation-mediated PCR.
abstract: Genomic sequencing permits studies of in vivo DNA methylation and protein-DNA interactions, but its use has been limited due to the complexity of the mammalian genome. Ligation-mediated PCR (LMPCR) is a sensitive genomic sequencing procedure that generates high quality, reproducible sequence ladders starting with only 1 microgram of uncloned mammalian DNA per reaction. This genomic sequencing procedure can be adapted for various methylation, in vivo footprinting and DNA adduct mapping procedures. We provide a detailed protocol for genomic sequencing by LMPCR and discuss the principles and applications of the method.
authors.affiliation: Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
authors.forename: G P
authors.initials: GP
authors.lastname: Pfeifer
authors.forename: A D
authors.initials: AD
authors.lastname: Riggs
mesh_terms: D018736:DNA Adducts; D018983:DNA Footprinting; D019175:DNA Methylation; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D018736:DNA Adducts