Flow cytometric analysis of the heat shock protein 60 expressed on the cell surface of Helicobacter pylori.
The expression of a 60-kDa heat shock protein (HSP60) on the cell surface of Helicobacter pylori was analysed by flow cytometry with polyclonal antibody directed to HSP60. All 13 strains of H. pylori examined expressed HSP60 on the cell surface, although the intensity of expression was different among the strains and depended on culture conditions. There was a correlation between the intensity of HSP60 expressed on the cell surface and the rate of adherence to human gastric carcinoma cells (MKN45) by H. pylori, but not with urease activity and production of vacuolating toxin. By flow cytometric analysis with monoclonal antibody (MAb) 3C8 against HSP60, the reactive epitope in the HSP60 of H. pylori was detected on the surface of MKN45 cells. Furthermore, it was shown that gastric epithelial cells were positively stained with MAb 3C8 in one of two biopsy specimens examined. These results suggest that there is a common epitope showing homology between H. pylori HSP60 and human gastric epithelial cells.
Yamaguchi
H
H
Department of Microbiology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Osaki
T
T
Taguchi
H
H
Hanawa
T
T
Yamamoto
T
T
Kamiya
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000096:biosynthesis; D002460:Cell Line; D018834:Chaperonin 60 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D003429:Cross Reactions; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D011817:Rabbits; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured; D014510:Urease / Q000032:analysis
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018834:Chaperonin 60; D000939:Epitopes; C074689:VacA protein, Helicobacter pylori; D014510:Urease