[Non-radioactive detection of dinucleotide repeat polymorphisms and its application to genetic diagnosis].
We have developed a rapid and non-radioactive procedure for detecting dinucleotide repeat polymorphisms. Specific primers flanking the repeats were used in the polymerase chain reaction (PCR). The generated allelic fragments were resolved by thin-layer denaturing polyacrylamide gel electrophoresis and visualized by improved silver staining. This method is simple, fast and without labelling of PCR products. Resolution of 1 bp can be easily obtained. It can also be used in multiplex PCR analysis of (CA)n loci. This method will be a valuable tool for linkage analysis and DNA diagnosis as demonstrated by its successful application to the CA dinucleotide repeat haplotying of a DMD family.
Huang
X
X
Division of Molecular Biology, Nanjing Railway Medical College.
Hu
X
X
Zong
H
H
Gao
Y
Y
D001483:Base Sequence; D018900:Dinucleotide Repeats / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000235:genetics; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic