Evaluation of survival and maturation of cryopreserved dopaminergic fetal cells transplanted into rat striatum and an analysis of the host brain reaction to graft.
A fetal, cryopreserved ventral mesencephalic rat tissue was transplanted into striatum of healthy adult rats. A stereotactic apparatus was used for transplantation of solid tissue blocks. The survival of transplanted dopaminergic cells in rat striatum was evaluated by means of histological and immunocytochemical methods (TH - thyrosine hydrolase) 1, 3, 7, 14, and 21 days after transplantation. The cellular reaction of the host to graft and to sham-lesion was examined. Glial fibrillary acidic protein (GFAP) was used for the visualization of astroglial reaction and ferritin for microglia. It was found that fetal cells of cryopreserved rat ventral mesencephalon transplanted into adult rat striatum survive though, in a small number. Cellular reactions of the host to both graft of dopaminergic cells and sham-lesion are similar to glial scar and are nonspecific.
Kosno-Kruszewska
E
E
Department of Neuropathology, Institute of Psychiatry and Neurology, Warszawa.
Wierzba-Bobrowicz
T
T
Ilnicki
K
K
Lechowicz
W
W
Dymecki
J
J
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D016380:Brain Tissue Transplantation; D003342:Corpus Striatum / Q000601:surgery*; D015925:Cryopreservation; D004298:Dopamine / Q000502:physiology*; D016332:Fetal Tissue Transplantation; D005904:Glial Fibrillary Acidic Protein; D006085:Graft Survival; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005904:Glial Fibrillary Acidic Protein; D004298:Dopamine