title: Sequence-specific binding and cleavage of duplex DNA by a radioiodinated, intercalator-linked, triplex-forming oligonucleotide.
abstract: Applications of oligodeoxynucleotides to modulate gene expression have been the subject of much recent research. We have sought to develop a method to permanently inactivate a gene, or potentially kill cells containing abnormal genes. In this report, we show that a DNA intercalator conjugated to a triplex-forming oligonucleotide can be labeled with an Auger electron emitting radioisotope, can cleave its duplex DNA target, and can specifically bind the target sequence contained in a total of 10 kilobases of irrelevant DNA.
authors.affiliation: Veterans Affairs Medical Center Research Center on AIDS and HIV Infections, Houston, TX, USA.
authors.forename: F M
authors.initials: FM
authors.lastname: Orson
authors.forename: W M
authors.initials: WM
authors.lastname: McShan
authors.forename: B M
authors.initials: BM
authors.lastname: Kinsey
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D007364:Intercalating Agents; D007457:Iodine Radioisotopes; D009841:Oligonucleotides; D004247:DNA