Field and pore size dependence of the electrophoretic mobility of DNA: a combination of fluorescence recovery after photobleaching and electric birefringence measurements.
By combining an electrophoretic cell with a setup of fluorescence recovery after photobleaching (FRAP) we can measure the electrophoretic mobility mu of double-stranded lambda DNA in agarose gel as a function of electric field E and gel concentration C. Mobility varies linearly with the field in agreement with the biased reptation model with fluctuations. The slopes are analyzed in term of orientation and compared with birefringence results. The mobility extrapolated at zero field follows the prediction of the reptation theory; we deduced the variation of the pore size with the agarose concentration. With a special use of our setup, we measure directly the free-mobility mu 0 of the DNA.
Tinland
B
B
Institut Charles Sadron, CNRS, University Louis Pasteur, Strasbourg, France. tinland@janus.u-strasbg.fr
Pernodet
N
N
Weill
G
G
D010582:Bacteriophage lambda / Q000235:genetics; D001583:Benzoxazoles / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis*; D004553:Electric Conductivity; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011806:Quinolinium Compounds / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry; D012996:Solutions; D013997:Time Factors
D001583:Benzoxazoles; D004279:DNA, Viral; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; D012996:Solutions; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D012685:Sepharose