Sequence-specific photomodification of single-stranded and double-stranded DNA fragments by oligonucleotide perfluoroarylazide derivative.
A highly efficient, sequence-specific photomodification of single-stranded (ss) and double-stranded (ds) DNA fragments was carried out with a hexadecathymidilate derivative, R approximately p(T)16 (R-perfluoroarylazido group), using 27-base pair DNA fragments as a target [table: see text] The main points of modification were G7 and G24 of the A-rich strand of the ss target and G7 and G22 of the A-rich and T-rich strands, respectively, for the ds target. The extent of photomodification was 60%-77% for ss DNA and 10%-53% for ds DNA depending on the reaction conditions. Photomodification increased in buffer with a high ionic strength (1.0 M) and at low temperature (4 degrees C) when presumably the triplexes were more stable.
Levina
A S
AS
Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Tabatadze
D R
DR
Dobrikov
M I
MI
Shishkin
G V
GV
Zarytova
V P
VP
D001386:Azides; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010777:Photochemistry; D017422:Sequence Analysis, DNA
D001386:Azides; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA