title: Effect of two levels of transgalactosylated oligosaccharide intake in rats associated with human faecal microflora on bacterial glycolytic activity, end-products of fermentation and bacterial steroid transformation.
abstract: The effects of two levels of transgalactosylated oligosaccharide (TOS) intake on bacterial glycolytic activity, end products of fermentation and bacterial steroid transformation were studied in rats associated with a human faecal flora. Rats were fed a human-type diet containing 0, 5 or 10% TOS. Caecal pH decrease correlated with the amount of TOS in the diet. Intake of the TOS diet induced a decrease in blood cholesterol and a strong increase in beta-galactosidase activity in the hindgut. TOS fermentation led to production of hydrogen and short chain fatty acids, whereas ammonia and branched-chain fatty acids were decreased. A diet containing 10% TOS increased caecal lactic acid concentrations and reduced beta-glucuronidase activities and steroid transformation.
authors.affiliation: Laboratoire d'Ecologie et Physiologie du Systeme Digestif, INRA-CRJ, Jouy en Josas, France.
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: C
authors.initials: C
authors.lastname: Andrieux
authors.forename: M
authors.initials: M
authors.lastname: Riottot
authors.forename: M
authors.initials: M
authors.lastname: Bensaada
authors.forename: F
authors.initials: F
authors.lastname: Popot
authors.forename: P
authors.initials: P
authors.lastname: Beaumatin
authors.forename: O
authors.initials: O
authors.lastname: Szylit
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D001835:Body Weight; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D005285:Fermentation / Q000187:drug effects; D005503:Food Additives / Q000494:pharmacology*; D005856:Germ-Free Life; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology*; D009929:Organ Size; D051381:Rats; D011916:Rats, Inbred F344; D013256:Steroids / Q000378:metabolism
chemical_list: D005503:Food Additives; D009844:Oligosaccharides; D013256:Steroids