A new microcatheter enables microcalorimetric assessment of thermogenesis after stimulation in cell cultures.
A new, inexpensive technique facilitates the observation of long-lasting effects (> 2 h) of thermogenic agents on cell cultures. A special microcatheter enables addition of hormones or pharmacologic agents to biological samples during microcalorimetric analysis using a ThermoMetric ThermalActivityMonitor without removing the sample ampoules from the measuring position. Technical modification of the calorimeter is not required. Baseline stability with the connected catheter is +/- 0.2 microW over 10 h. The error of the method of catecholamine induced thermogenesis in white adipocytes is 13.4% (C.V.).
Böttcher
H
H
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany.
Fürst
P
P
D017667:Adipocytes / Q000378:metabolism*; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D002404:Catheterization; D002478:Cells, Cultured; D007545:Isoproterenol / Q000494:pharmacology
D007545:Isoproterenol