title: Use of morpholinonucleosides to conjugate oxidized DNA bases to proteins.
abstract: The universal method of Erlanger and Beiser was unsuccessful in conjugating 5-hydroxycytidine to proteins because of the instability of the base under the conditions used. A new strategy was developed to conjugate fragile modified nucleosides to proteins. This involves the use of morpholino derivatives of modified nucleosides. The validity of this method was demonstrated by the production of polyclonal antibodies specific for the DNA modification, 8-oxo-7,8-dihydro-2'-deoxyguanosine.
authors.affiliation: CEA/Département de Recherche Fondamentale sur la Matière Condensée, SESAM/LAN, Grenoble, France.
authors.forename: I
authors.initials: I
authors.lastname: Girault
authors.forename: D E
authors.initials: DE
authors.lastname: Shuker
authors.forename: J
authors.initials: J
authors.lastname: Cadet
authors.forename: D
authors.initials: D
authors.lastname: Molko
mesh_terms: D000906:Antibodies; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives* / Q000276:immunology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals; D006147:Guanine / Q000031:analogs & derivatives / Q000276:immunology; D013058:Mass Spectrometry
chemical_list: D000906:Antibodies; D005609:Free Radicals; C024829:8-hydroxyguanine; D006147:Guanine; C017400:5-hydroxycytosine; D003596:Cytosine; D004247:DNA