Radioiodinated (aminostyryl)pyridinium (ASP) dyes: new cell membrane probes for labeling mixed leukocytes and lymphocytes for diagnostic imaging.
We prepared [125I/131I]iodo-(aminostyryl)pyridinium dyes from tributylstannyl precursors. ASP 7a and 7b labeled leukocytes ex vivo (70-94%) using saline with or without washing plasma from cells. Viability of peripheral blood lymphocytes (PBLs) (dogs, rats) and splenic lymphocytes (rats) labeled with 7a and 7b (71-82%) was unchanged after labeling (> or = 88%). Canine 7b-leukocytes showed higher uptake in inflammatory lesions than did 111In-oxine leukocytes. At 3 h, aspirates contained more radioiodine than 111In (1.65:1 to 22:1) and radioiodine was cell bound. ROI measurements (3 h) gave abscess to contralateral knee ratios of 12.3 and 10.6 for 131I-7b vs. 4.8 and 2.3 for 111In-oxine.
Lambert
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Department of Radiology, George Washington University Medical Center, Washington, DC 20037, USA.
Mease
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Avren
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Sabet
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McAfee
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D000818:Animals; D002462:Cell Membrane / Q000000981:diagnostic imaging; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004285:Dogs; D007249:Inflammation / Q000097:blood / Q000139:chemically induced; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods; D007962:Leukocytes / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000648:ultrastructure; D008214:Lymphocytes / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000648:ultrastructure; D011726:Pyridinium Compounds / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D051381:Rats; D013343:Styrenes / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D014220:Trialkyltin Compounds / Q000737:chemistry
D004396:Coloring Agents; D007457:Iodine Radioisotopes; D011726:Pyridinium Compounds; D013343:Styrenes; D014220:Trialkyltin Compounds; C011559:tributyltin