Immediate early gene c-fos regulates the synthesis of phospholipids but not of gangliosides.
Retinal ganglion cells isolated from chicks that in vivo were exposed to light have a higher phospholipid labeling capacity than those obtained from animals in the dark. Actinomycin D or a mixture of protein synthesis inhibitors or of antisense oligonucleotides to c-fos plus c-jun injected intraocularly 1 hr prior to the stimulation period, abolished the light-dark differences for phospholipids but not for gangliosides. Light stimulation induced the formation (and/or stabilization) of c-fos mRNA and of the protein c-Fos, indicating that immediate early gene induction, and consequently the synthesis of the protein(s) encoded, is essential to increase the synthesis of phospholipids but not of gangliosides. These results suggest a novel mechanism by which immediate early genes engram neural cells, modifying specifically the metabolism of cell constituents producing long-lasting changes in the cells.
Guido
M E
ME
CIQUIBIC (CONICET), Departamento de Química Biológica, Universidad Nacional de Córdoba, Argentina.
de Arriba Zerpa
G A
GA
Bussolino
D F
DF
Caputto
B L
BL
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003609:Dactinomycin / Q000494:pharmacology; D005732:Gangliosides / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000528:radiation effects; D017781:Genes, Immediate-Early; D016762:Genes, fos; D008027:Light; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000528:radiation effects; D015398:Signal Transduction / Q000528:radiation effects; D015533:Transcriptional Activation
D005732:Gangliosides; D016376:Oligonucleotides, Antisense; D010743:Phospholipids; D011500:Protein Synthesis Inhibitors; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D003609:Dactinomycin