Photoaffinity labeling of HIV reverse transcriptase: utilization of 2',3'-dideoxyuridylate analog bearing aryl(trifluoromethyl)diazirine moiety as a chain terminator.
In order to develop a photoaffinity labeling reagent for HIV-1 reverse transcriptase, we utilized 2',3'-dideoxy-E-5-[4-[3-(trifluoromethyl)-3H-diazirin-3-yl] styryl]UTP (TDS-ddUTP) for formation of a photolabile primer. This compound was incorporated into the 3'-terminus of the primer by reverse transcriptase activity, and thus the generated photo-reactive primer was able to bind to the enzyme molecule by photo-irradiation.
Yamaguchi
T
T
Department of Biological Sciences, Nishi-Tokyo University, Yamanashi, Japan.
Saneyoshi
M
M
D000345:Affinity Labels; D001389:Azirines / Q000737:chemistry; D001665:Binding Sites; D003856:Deoxyuracil Nucleotides / Q000737:chemistry; D054306:Dideoxynucleotides; D054303:HIV Reverse Transcriptase / Q000737:chemistry* / Q000528:radiation effects; D015394:Molecular Structure; D010777:Photochemistry; D014466:Ultraviolet Rays
C101663:2',3'-dideoxy-5-(4-(3-trifluoromethyl-3H-diazirin-3-yl)styryl)uridine triphosphate; D000345:Affinity Labels; D001389:Azirines; D003856:Deoxyuracil Nucleotides; D054306:Dideoxynucleotides; D054303:HIV Reverse Transcriptase