Somatostatin receptors in the developing rat brain.
The distribution of [125I]SRIF-28 ([Leu8,D-Trp22,125I-Tyr25]somatostatin-28), [125I]204-090 ([Tyr3]octreotide) and [125I]CGP 23996 (c[Asu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Tyr-Thr-Ser]) labelled recognition sites was studied by autoradiography in rat brain at embryonic day 18 (E 18) and postnatal day 5 (P 5). These results were compared with mRNA expression of somatostatin receptors SSTR1-5 (named sst1-5 now) as studied by in situ hybridization. [125I]SRIF-28, [125I]204-090 and [125I]CGP 23996 binding displayed different although partially overlapping distributions, and showed an increase between E 18 and P 5, which was less marked for [125I]204-090 binding. -125I-204-090 binding and sst2 receptor mRNA were similarly distributed, whereas [125I]CGP 23996 binding did not correlate with any single somatostatin receptor mRNA. The data suggest that most SRIF receptor subtypes in rat brain are present before birth, but evolve differently.
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Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
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Hoyer
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D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006728:Hormones / Q000494:pharmacology; D017403:In Situ Hybridization; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D015282:Octreotide / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000187:drug effects / Q000378:metabolism*; D013004:Somatostatin / Q000031:analogs & derivatives / Q000494:pharmacology; D053561:Somatostatin-28
D006728:Hormones; D007457:Iodine Radioisotopes; D017481:Receptors, Somatostatin; D013004:Somatostatin; D053561:Somatostatin-28; C038136:CGP 23996; D015282:Octreotide