Esterase D (ESD EC 3.1.1.1) polymorphism in hemolysates and bloodstains. II. Comparison of ESD stability in dried bloodstains in relation to the temperature of storage and the type and character of substrate.
The aim of the study was evaluation of the possibility of ESD phenotyping in simulated casework bloodstains in relation to the temperature of storage and to the type and character of substrate. Bloodstained materials were exposed to extreme temperature conditions including -20 degrees C, 4 degrees C, 22 degrees C and 45 degrees C and subsequently phenotyped with the use of electrophoresis and isoelectric focusing. Presented results confirm faster decrease of the enzyme total activity in the conditions of increased temperature manifesting with relatively lower fluorescence and resolution of electrophoretic bands. Preliminary evaluation of external conditions and recognition of the type of the substrate prior to casework bloodstain analysis is suggested.
Pepiński
W
W
Department of Forensic Medicine, Medical Academy of Białystok.
Janica
J
J
Skawrońska
M
M
Nałecka
M
M
D001801:Blood Stains; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D004795:Enzyme Stability; D006461:Hemolysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic; D013379:Substrate Specificity; D013696:Temperature; D014021:Tissue Preservation
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human