Relative responses of protein turnover in three different skeletal muscles to dietary lysine deficiency in chicks.
1. The effect of lysine deficiency was analysed on muscle protein turnover in 2-, 3- and 4-week-old growing broilers. Protein fractional synthesis rates (FSR, in %/d) were measured by a reliable in vivo technique (flooding dose of L-[4-3H] phenylalanine) in the Pectoralis major (PM), the Anterior latissimus dorsi (ALD) and the Sartorius (SART) muscles. Protein fractional breakdown rates (FBR, in %/d) were estimated as the difference between the synthesis rates and the growth rates of tissue protein. 2. Lysine deficiency resulted in significant increases in muscle FSR and FBR. When expressed in absolute rates (g/d), tissue protein deposition was reduced whatever the tissue. This phenomenon was accompanied by decreased protein synthesis (ASR). 3. The protein turnover responsiveness to the lysine deficiency appeared to depend on the studied muscle, since the PM muscle was the most sensitive whereas the SART and ALD muscles presented a lower sensitivity.
Tesseraud
S
S
Station de Recherches Avicoles, Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly, France.
Maaa
N
N
Peresson
R
R
Chagneau
A M
AM
D000375:Aging / Q000502:physiology; D000704:Analysis of Variance; D000821:Animal Feed; D000818:Animals; D002645:Chickens; D004032:Diet; D008239:Lysine / Q000172:deficiency*; D008297:Male; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism*; D009929:Organ Size; D009928:Organ Specificity; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D014316:Tritium
D014316:Tritium; D010649:Phenylalanine; D008239:Lysine