Evidence for a pseudoknot in the 3' untranslated region of the bovine coronavirus genome.
A potential pseudoknot was found in the 3' untranslated region of the bovine coronavirus genome beginning 63 nt downstream from the stop codon of the N gene. Mutation analysis of the pseudoknot in a cloned defective interfering RNA indicated that this structural element is necessary for defective interfering RNA replication.
Williams
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Program in Biotechnology, University of Tennessee, Knoxville 37996-0845, USA.
Chang
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Brian
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D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D017938:Coronavirus, Bovine / Q000235:genetics* / Q000378:metabolism; D003673:Defective Viruses / Q000235:genetics / Q000378:metabolism; D005814:Genes, Viral; D016679:Genome, Viral; D008961:Models, Structural; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000096:biosynthesis* / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D005760:Transmissible gastroenteritis virus / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics*
D003062:Codon; D012367:RNA, Viral; D014758:Viral Core Proteins