Assessment of variability in some lysosomal and soluble enzyme activities in corneal epithelium from slaughterhouse bovine eyes.
The corneal epithelium was scraped from bovine eyes (Hereford or Holstein) within 3 h post mortem. The mitochondriallysosomal fraction of the cells was assayed for hexosaminidase (HEX) and acid phosphatase (AP), and the membrane-soluble fraction for lactate dehydrogenase (LDH) and leucine arylamidase (LAA) activity. Pairs of eyes with clear corneas showed variances (+/-1 SD, n = 10 pairs) of +/-62, +/-57, +/-72 and +/-31% in the four enzyme activities (HEX, AP, LDH, LAA). Selection based on age (corneal diameter) reduced the variance to +/-41, +/-37, +/-39 and +/-30%. Additional selection for corneal surface wettability reduced variances to +/-26, +/-21, +/-22 and +/-26% in the activities. The latency of the lysosomal enzymes (HEX, AP) increased substantially with eye selection.
Doughty
M J
MJ
Glasgow-Caledonian University, Department of Vision Science, Scotland, UK.
D000003:Abattoirs; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003315:Cornea / Q000166:cytology / Q000201:enzymology*; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007931:Leucyl Aminopeptidase / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase; D001619:beta-N-Acetylhexosaminidases; D007931:Leucyl Aminopeptidase