The role of receptor protein tyrosine phosphatase alpha in neuronal differentiation of embryonic stem cells.
In the present study, we have investigated the function of the receptor protein tyrosine phosphatase alpha (RPTP alpha) in the neuronal differentiation of E14-embryonic stem (E14-ES) cells. RNAase protection and western blot analysis revealed that E14-ES cells up regulate RPTP alpha expression upon neuronal differentiation with retinoic acid. Overexpression of RPTP alpha, by stable DNA transfection, and subsequent differentiation with retinoic acid, resulted in a temporally enhanced expression of the neuronal markers GAP-43 and NF-164. Electrophysiological experiments demonstrated that RPTP alpha overexpression also enhanced the development of neurotransmitter responses during differentiation. These results indicate that RPTP alpha plays an important role in the cascade of molecular events that lead to the formation of neurons.
van Inzen
W G
WG
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Peppelenbosch
M P
MP
van den Brand
M W
MW
Tertoolen
L G
LG
de Laat
S
S
D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D017027:Protein Tyrosine Phosphatases / Q000502:physiology*; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D011956:Receptors, Cell Surface / Q000502:physiology*; D014162:Transfection
D015415:Biomarkers; D011956:Receptors, Cell Surface; C516841:PTPRA protein, human; D017027:Protein Tyrosine Phosphatases; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4