Involvement of carnitine acyltransferases in peroxisomal fatty acid metabolism by the yeast Pichia guilliermondii.
This article provides information about peroxisomal fatty acid metabolism in the yeast Pichia guilliermondii. The existence of inducible mitochondrial carnitine palmitoyltransferase and peroxisomal carnitine octanoyl-transferase activities was demonstrated after culture of this yeast in a medium containing methyl oleate. The subcellular sites and induction patterns were studied. The inhibition of carnitine octanoyl- and palmitoyl-transferases by chlorpromazine to a large extent prevented the otherwise observed metabolism-dependent inactivation of thiolase by 2-bromofatty acids in vivo. We concluded that the metabolism of long- and medium-chain fatty acids in the peroxisome of this yeast involved carnitine intermediates.
Pagot
Y
Y
Laboratoire de Biotechnologie, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Campus Universitaire Montmuzard, Dijon, France.
Belin
J M
JM
D000101:Acetyl-CoA C-Acetyltransferase / Q000037:antagonists & inhibitors; D018533:Biomass; D002333:Carnitine Acyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D009829:Oleic Acids / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000378:metabolism; D013104:Spheroplasts
D004791:Enzyme Inhibitors; D005227:Fatty Acids; D009829:Oleic Acids; C005576:methyl oleate; D002333:Carnitine Acyltransferases; D000101:Acetyl-CoA C-Acetyltransferase; D002746:Chlorpromazine