title: Mechanism of antisense effect studied by functionalized oligonucleotides.
abstract: In order to study the mechanism of antisense effects, various functional groups such as photocrosslinkers, fluorescent materials, and gold particles were introduced to antisense DNAs. Upon UV-irradiation, the hybrid between DNA and antisense DNA with a photocrosslinker was stabilized. The fluorescence microscopy and transmission electron microscopy (TEM) represented clear images of antisense DNA in the cultured cells. Fluorescent-labeled S-Oligos were recognized in the cytoplasm and especially on certain organs, which are not specified. TEM images of cultured cells treated with gold-conjugated S-Oligo suggested the mechanism of cellular uptake of antisense DNA.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: A
authors.initials: A
authors.lastname: Yoshimoto
authors.forename: A
authors.initials: A
authors.lastname: Furumoto
authors.forename: M
authors.initials: M
authors.lastname: Namba
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003432:Cross-Linking Reagents; D016650:Fluorescein-5-isothiocyanate; D006046:Gold; D015394:Molecular Structure; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D010777:Photochemistry; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense; D006046:Gold; D016650:Fluorescein-5-isothiocyanate