Location of the alpha-amylase gene in rumen Streptococcus bovis strains distinguished by unstable amylase activity.
Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal Streptococcus bovis strains was investigated. Two strains Amy+ and Amy- were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pJK108 against chromosomal DNA of S. bovis and Bacillus subtilis after a complete restriction with EcoRI.
Mareková
M
M
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
Jonecová
Z
Z
Kmeĭ
V
V
D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004795:Enzyme Stability / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D012417:Rumen / Q000382:microbiology; D016985:Streptococcus bovis / Q000201:enzymology* / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D004269:DNA, Bacterial; D000516:alpha-Amylases