title: Immunohistochemical localization of Lyn (p56) protein in the adult rat brain.
abstract: Expression of a sarcoma proto-oncogene, c-lyn, was mapped in the adult rat brain using immunohistochemistry. Lyn protein was prevalent in restricted cell populations of the olfactory bulb and the basal forebrain which included nuclei of accumbens, fundal striatum, bed stria, ventral pallidum and central amygdala as well as deep entorhinal and pyriform cortices. Tightly packed Lyn-positive cells formed discrete multiple stripes crossing perpendicular to the rostral limb of the anterior commissure, and intense masses surrounding the caudal limb. In the thalamus, the habenula, anterodorsal nucleus and medial geniculate body, together with the paraventricular hypothalamic nuclei, had prominent reactive neuronal somata and dendrites in the neuropil. The lateral septal nucleus also had intense Lyn-positive neurons with overlapping dendritic fields. In addition, scattered neurons were evenly distributed throughout the striatum. The red, interpeduncular, auditory and trigeminal tract nuclei were intensely reactive. The cerebellar molecular layer was uniformly labeled except for a few isolated fiber bundles in the lowest part of this layer. The granule cells adjacent to the Purkinje cell layer appeared in reactive patches. In the spinal cord, the posteromarginal nucleus had intense labeling. The significance of this highly localized distribution pattern of Lyn protein may be related to connections forming functional compartments serving signal transduction within specific central nervous system circuitry.
authors.affiliation: Department of Otolaryngology and Physiology, New York University Medical Center, NY 10016, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Bing
authors.forename: N
authors.initials: N
authors.lastname: Rosenblum
authors.forename: D E
authors.initials: DE
authors.lastname: Hillman
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology / Q000201:enzymology; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D019061:src-Family Kinases / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases