Cystatin C and apolipoprotein E immunoreactivities in CA1 neurons in ischemic gerbil hippocampus.
The distribution patterns of cystatin C and apolipoprotein E (apo E) were studied immunocytochemically in the gerbil hippocampus before and after 5 min ischemia. In the controls, cystatin C was distributed mainly in astrocytes. In addition, a large number of dots positive for cystatin C were observed around the outlines of neuronal perikarya in the CA1 subfields. One day after ischemia, cystatin C-positive stainings outlining neuronal cell bodies disappeared. On the fourth day, intense stainings for cystatin C appeared in atrophied pyramidal neurons and these stainings in neurons disappeared by the 14th day. A remarkable increase in the number of cystatin C-positive astrocytes occurred on the fourth day and thereafter these spread over the whole of the CA1 subfield. Apo E was also distributed in astrocytes in the control specimens. From the fourth day, extra- and/or intracellular distribution of apo E-immunoreactivities was noted in the stratum pyramidale. Apo E-positive astrocytes disappeared transiently on the fourth day and then reappeared and increased remarkably by the 14th day. These findings indicate that cystatin C and apo E are involved in the degeneration process of brain neuronal cells.
Ishimaru
H
H
Department of Neuropsychopharmacology (Tsumura), Gunma University School of Medicine, Japan.
Ishikawa
K
K
Ohe
Y
Y
Takahashi
A
A
Maruyama
Y
Y
D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D055316:Cystatin C; D015891:Cystatins / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism*; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D017966:Pyramidal Cells / Q000473:pathology; D013997:Time Factors
D001057:Apolipoproteins E; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D005904:Glial Fibrillary Acidic Protein