title: Tissue distribution of adenosine receptor mRNAs in the rat.
abstract: 1. A degree of ambiguity and uncertainty exists concerning the distribution of mRNAs encoding the four cloned adenosine receptors. In order to consolidate and extent current understanding in this area, the expression of the adenosine receptors has been examined in the rat by use of in situ hybridisation and the reverse transcription-polymerase chain reaction (RT-PCR). 2. In accordance with earlier studies, in situ hybridisation revealed that the adenosine A1 receptor was widely expressed in the brain, whereas A2A receptor mRNA was restricted to the striatum, nucleus accumbens and olfactory tubercle. In addition, A1 receptor mRNA was detected in large striatal cholinergic interneurones, 26% of these neurones were also found to express the A2A receptor gene. Central levels of mRNAs encoding adenosine A2B and A3 receptors were, however, below the detection limits of in situ hybridisation. 3. The more sensitive technique of RT-PCR was then employed to investigate the distribution of adenosine receptor mRNAs in the central nervous system (CNS) and a wide range of peripheral tissues. As a result, many novel sites of adenosine receptor gene expression were identified. A1 receptor expression has now been found in the heart, aorta, liver, kidney, eye and bladder. These observations are largely consistent with previous functional data. A2A receptor mRNA was detected in all brain regions tested, demonstrating that expression of this receptor is not restricted to the basal ganglia. In the periphery A2A receptor mRNA was also found to be more widely distributed than generally recognised. The ubiquitous distribution of the A2B receptor is shown for the first time, A2B mRNA was detected at various levels in all rat tissues studied. Expression of the gene encoding the adenosine A3 receptor was also found to be widespread in the rat, message detected throughout the CNS and in many peripheral tissues. This pattern of expression is similar to that observed in man and sheep, which had previously been perceived to possess distinct patterns of A3 receptor gene expression in comparison to the rat. 4. In summary, this work has comprehensively studied the expression of all the cloned adenosine receptors in the rat, and in so doing, resolves some of the uncertainty over where these receptors might act to control physiological processes mediated by adenosine.
authors.affiliation: Department of Pharmacology, University of Cambridge.
authors.forename: A K
authors.initials: AK
authors.lastname: Dixon
authors.forename: A K
authors.initials: AK
authors.lastname: Gubitz
authors.forename: D J
authors.initials: DJ
authors.lastname: Sirinathsinghji
authors.forename: P J
authors.initials: PJ
authors.lastname: Richardson
authors.forename: T C
authors.initials: TC
authors.lastname: Freeman
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018047:Receptors, Purinergic P1 / Q000096:biosynthesis* / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D018047:Receptors, Purinergic P1