Cryopreservation of sheathed third-stage larvae of Nematodirus spathiger.
A technique for the cryopreservation of sheathed third-stage larvae Nematodirus spathiger is described. It consists of incubating larvae in 5% (v/v) ethylene glycol for 20 min at room temperature, followed by 1 min of incubation in 40% (v/v) ethylene glycol at 0 degrees C. The average recovery of adult worms was 28.5% for two adult sheep infected with these larvae.
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Helminthology Section, Onderstepoort Veterinary Institute, South Africa.
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D007814:Larva; D008297:Male; D009348:Nematoda / Q000472:pathogenicity*; D012756:Sheep / Q000469:parasitology