Hermes, a functional non-Drosophilid insect gene vector from Musca domestica.
Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster while (w+) gene and created D. melanogaster germline transformants. Transgenic G1 insects were recovered from 34.6% of the fertile G0 adults developing from microinjected w- embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster, however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance.
O'Brochta
D A
DA
Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park 20742, USA. dol4@umail.umd.edu
Warren
W D
WD
Saville
K J
KJ
Atkinson
P W
PW
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D017344:Genes, Insect; D005822:Genetic Vectors / Q000235:genetics*; D006793:Houseflies / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004251:DNA Transposable Elements