Altered glycine transport by cerebral tissue and decreased Na+ and Ca++ pump activities during organophosphorus-ester-induced delayed neurotoxicity development period.
Uptake of [U-14C] glycine during the organophosphorus-ester-induced delayed neurotoxicity (OPIDN) development period was studied. Diisopropyl fluorophosphate (DFP), a delayed neurotoxic organophosphorus ester was administered to adult rats and hens. Results showed a decreased accumulation of glycine in hen cerebral cortex slices during the delayed neurotoxicity development period. An altered sensitivity toward transport inhibitors 2,4-dinitrophenol and ouabain was observed in DFP-treated hens. An altered neuronal membrane function during the OPIDN development period is reported in the present work. Brain Na+, K(+)-ATPase and Ca(++)-ATPase activities decreased during the neurotoxicity development period. The decrease in Ca(++)-ATPase activity persisted in hens until the complete development of neurotoxic symptoms. Decreased Ca++ pump activity is correlated with altered membrane function during OPIDN.
Sharma
S K
SK
Biochemistry Division, Defence Research and Development Establishment, Gwalior, India.
Bhattacharya
B K
BK
D019297:2,4-Dinitrophenol; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D004140:Dinitrophenols / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005998:Glycine / Q000378:metabolism*; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D009422:Nervous System Diseases / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
D004140:Dinitrophenols; D004791:Enzyme Inhibitors; D009943:Organophosphorus Compounds; D011480:Protease Inhibitors; D014475:Uncoupling Agents; D007531:Isoflurophate; D010042:Ouabain; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D019297:2,4-Dinitrophenol; D005998:Glycine