PSK protects macrophages from lipoperoxide accumulation and foam cell formation caused by oxidatively modified low-density lipoprotein.
In previous works, it has been evidenced that lipoperoxidative injury to macrophages caused by oxidatively modified low-density lipoprotein (O-LDL) plays an important role in foam cell formation, and that PSK, a protein bound polysaccharide extracted from the class Basidiomycetes Coriolus Versicolor, can protect macrophages from lipoperoxidative injury induced by tert-butyl hydroperoxide (tbOOH). In this paper PSK protection of macrophages from lipoperoxide (LPO) accumulation and foam cell formation caused by O-LDL and its action mechanism were further studied. The LPO accumulation was determined by using ACAS 570. Dynamic assay of the LPO level in eight single cells after adding O-LDL or determination of the average LPO content in a lot of cells incubated in advance with O-LDL for 12 h, both indicated that O-LDL might induce LPO accumulation in macrophages and the effects of O-LDL could be prevented by PSK. O-LDL might cause the changes of morphological structure in macrophages and the transformation of macrophages into foam cells, and the effects could also be prevented by PSK. The determination of selenium-dependent glutathione peroxidase (SeGSHPx) activities and mRNA contents of macrophages and changes of SeGSHPx activity and mRNA content after incubation with tbOOH showed that PSK might increase the SeGSHPx activity of macrophage and the enhanced SeGSHPx activity may occur at the level of gene transcription.
Yuan
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C
Research Laboratory of Free Radical Medicine, First Military Medical University, Guangzhou, China.
Mei
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Z
Liu
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S
Yi
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L
D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D005487:Foam Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D010545:Peroxides / Q000009:adverse effects; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D014461:Ultracentrifugation; D020122:tert-Butylhydroperoxide
D007155:Immunologic Factors; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D010545:Peroxides; D011509:Proteoglycans; D012333:RNA, Messenger; C010770:polysaccharide-K; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase