Analysis of binary mixtures of cephalothin and cefoxitin by using first-derivative spectrophotometry.
A method for the analysis of two-component mixtures of cephalothin and cefoxitin using zero-crossing first-derivative spectrophotometry is described. This technique permits the quantification of these drugs with closely overlapping spectral bands without any separation step. Linear calibration graphs of first-derivative values at 235.00 and 236.75 nm for cephalothin and cefoxitin, respectively, with negligible intercepts were obtained versus concentration in the range 4.0-32.0 micrograms ml-1 for both antibiotics. This paper presents a systematic examination of the experimental data by applying an exhaustive statistical analysis to demonstrate the validity of the method. The results of the determination of these antibiotics in mixtures of injectable dosage forms are also presented, together with their determinations in physiological serum and glucosed physiological serum.
Murillo
J A
JA
Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain.
Lemus
J M
JM
García
L F
LF
D002138:Calibration; D002440:Cefoxitin / Q000032:analysis / Q000097:blood / Q000737:chemistry; D002511:Cephalosporins / Q000032:analysis / Q000097:blood / Q000737:chemistry; D002512:Cephalothin / Q000032:analysis / Q000097:blood / Q000737:chemistry; D002513:Cephamycins / Q000032:analysis / Q000097:blood / Q000737:chemistry; D004359:Drug Therapy, Combination / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002511:Cephalosporins; D002513:Cephamycins; D007202:Indicators and Reagents; D012996:Solutions; D002440:Cefoxitin; D002512:Cephalothin