An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells.
Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.
Regner
M
M
Department of Medical Anatomy, Panum Institute, University of Copenhagen, Denmark.
Claësson
M H
MH
Bregenholt
S
S
Röpke
M
M
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence / Q000235:genetics; D002460:Cell Line; D015789:HLA-A2 Antigen / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008285:Major Histocompatibility Complex / Q000235:genetics; D009975:Orthomyxoviridae / Q000378:metabolism; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects*; D014763:Viral Matrix Proteins / Q000378:metabolism / Q000494:pharmacology
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D015789:HLA-A2 Antigen; D010455:Peptides; C073119:TAP1 protein, human; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins; C071692:TAP2 protein, human