title: Enzymatic synthesis of 4-O-beta-D-galactopyranosyl-moranoline and 3-O-beta-D-galactopyranosyl-moranoline by using beta-galactosidase from Bacillus circulans.
abstract: A transgalactosylation reaction from lactose to moranoline (1-deoxynojirimycin) was accomplished by using beta-galactosidase [EC 3.2.1.23] from Bacillus circulans. The enzyme formed 3-O-beta-D-galactopyranosyl-moranoline and 4-O-beta-D-galactopyranosyl-moranoline as major products, together with 2-O-beta-D-galactopyranosyl-moranoline and 6-O-beta-D-galactopyranosyl-moranoline as minor ones.
authors.affiliation: Discovery Research Lab. III, Nippon Shinyaku Co., Ltd., Kyoto Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Seto
authors.forename: Y
authors.initials: Y
authors.lastname: Kyotani
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Kitazawa
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Maruo
authors.forename: T
authors.initials: T
authors.lastname: Ohgi
authors.forename: Y
authors.initials: Y
authors.lastname: Ezure
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: C101238:3-O-galactopyranoylmoranoline; C101237:4-O-galactopyranosylmoranoline; D017485:1-Deoxynojirimycin; D001616:beta-Galactosidase