Twelve-well culture plate for the efficient collection and culture of human oocytes and embryos.
OBJECTIVE
Our goal was to assess a 12-well oocyte collection and embryo culture plate for use in the IVF laboratory.


METHODS
This was a prospective nonrandomized study.


METHODS
The setting was a university in vitro fertilization program.


METHODS
Eighty-four consecutive infertility couples presenting for IVF were studied.


METHODS
The main outcome measure was pregnancy (delivered).


RESULTS
A 34% delivered pregnancy rate per retrieval was attained using the 12-well collection and culture plate without the use of expensive culture media and special serum supplementation.


CONCLUSIONS
The use of a 12-well plate for oocyte collection and embryo culture provides a simple, economical, efficient, and effective means of producing human embryos during in vitro fertilization. This system is capable of supporting high rates of ongoing and delivered pregnancies.
Drury
K C
KC
Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville 32610, USA.
Konicek
J R
JR
Kipersztok
S
S
Williams
R S
RS
D000328:Adult; D046508:Culture Techniques / Q000295:instrumentation*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D011446:Prospective Studies