A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene.
The gene XYL1, encoding a xylose reductase, from Pichia stipitis was transformed into a mutant of Saccharomyces cerevisiae incapable of glycerol production because of deletion of the genes GPD1 and GPD2. The transformed strain was capable of anaerobic glucose conversion in the presence of added xylose, indicating that the xylose reductase reaction can fulfill the role of the glycerol-3-phosphate dehydrogenase reaction as a redox sink. The specific xylitol production rate obtained was 0.38 g g-1 h-1.
Lidén
G
G
Department of Chemical Reaction Engineering, Chalmers University of Technology, Göteborg, Sweden. liden@cre.chalmers.se
Walfridsson
M
M
Ansell
R
R
Anderlund
M
M
Adler
L
L
Hahn-Hägerdal
B
B
D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D000693:Anaerobiosis; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000502:physiology*; D010084:Oxidation-Reduction; D010843:Pichia / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic; D014993:Xylitol / Q000096:biosynthesis; D014994:Xylose / Q000378:metabolism*
D014994:Xylose; D005993:Glycerolphosphate Dehydrogenase; D000449:Aldehyde Reductase; D005947:Glucose; D014993:Xylitol