Methylmercury transport across the placenta via neutral amino acid carrier.
Methylmercury (MeHg) penetrates the placental barrier to affect developing fetuses in the uterus. However, the mechanism of placental MeHg transport is not well defined. To clarify the MeHg transport system that functions in the placenta, pregnant rats were intravenously administered MeHg on day 18 of gestation. The fetal blood was collected from the umbilical cord at 30 and 60 min after the administration, and its mercury concentration was measured. MeHg was found to be rapidly transported to the fetal blood in a time- and dose-dependent manner, and predominantly distributed in the blood cells there. MeHg transport was effectively suppressed by the co-injection of neutral amino acids, i.e., L-methionine and L-phenylalanine, suggesting that MeHg is actively transported as its cysteine conjugate via the neutral amino acid carrier system. The suppression by methionine was not so marked as by phenylalanine. Since methionine administration caused a rapid increase of the cysteine, which functioned as a predominant carrier in MeHg transport, in the maternal plasma, newly synthesized cysteine seemed to accelerate the mercury uptake. Accordingly, the acceleration by the extra cysteine would compensate partly the competitive effect of methionine as a neutral amino acid.
Kajiwara
Y
Y
Pathology Section, National Institute for Minamata Disease, Minamata City, Japan.
Yasutake
A
A
Adachi
T
T
Hirayama
K
K
D026905:Amino Acid Transport Systems; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D008431:Maternal-Fetal Exchange; D008628:Mercury / Q000097:blood; D008767:Methylmercury Compounds / Q000493:pharmacokinetics*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000097:blood; D014528:Uricosuric Agents / Q000494:pharmacology
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D008767:Methylmercury Compounds; D013438:Sulfhydryl Compounds; D014528:Uricosuric Agents; D008628:Mercury; D011339:Probenecid