Inhibition of the induction of collagenase by interleukin-1 beta in cultured rabbit synovial fibroblasts after treatment with the poly(ADP-ribose)-polymerase inhibitor 3-aminobenzamide.
3-Aminobenzamide is an inhibitor of poly-(ADP-ribosyl)ation. In concentrations from 3 to 10 mM it reduced the collagenase activity in culture supernatants of interleukin-1 beta-stimulated rabbit synovial fibroblasts. 3-Aminobenzoate, not an inhibiter of poly(ADP-ribosyl)ation, had no effect on collagenase activity at a concentration of 10 mM. We concluded that poly(ADP-ribosyl)ation plays a role in the induction of the expression of collagenase and that 3-aminobenzamide can inhibit this process.
Ehrlich
W
W
Abteilung Biochemie, Robert-Koch-Institut, Berlin, Germany.
Huser
H
H
Kröger
H
H
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism
D001549:Benzamides; D004791:Enzyme Inhibitors; D007375:Interleukin-1; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; D017364:Collagenases