title: Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides.
abstract: Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SgII in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is approximately 4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.
authors.affiliation: European Institute for Peptide Research (IFRMP No. 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Anouar
authors.forename: S
authors.initials: S
authors.lastname: Jégou
authors.forename: D
authors.initials: D
authors.lastname: Alexandre
authors.forename: I
authors.initials: I
authors.lastname: Lihrmann
authors.forename: J M
authors.initials: JM
authors.lastname: Conlon
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D002864:Chromogranins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D010902:Pituitary Gland / Q000737:chemistry; D011498:Protein Precursors / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification; D011895:Rana ridibunda / Q000235:genetics*; D053381:Secretogranin II; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D002864:Chromogranins; D018076:DNA, Complementary; D009479:Neuropeptides; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D053381:Secretogranin II; C081159:secretoneurin