Abnormal B cell response of protein kinase C in some common variable immunodeficiency.
The localization of protein kinase C (PKC) was investigated in B cells or B cell lines derived from patients with common variable immunodeficiency (CVI) and healthy controls. Stimulation of the healthy control B cells by phorbol ester or anti-mu induced PKC activation and translocation to the plasma membrane after 10 min. In contrast, in the CVI-B cells, no apparent PKC translocation was induced by either phorbol ester or anti-mu stimulation. These results suggest that the pathogenesis in some CVI patients might involve defects in the pathway of PKC activation.
Kaneko
H
H
Department of Pediatrics, Gifu University School of Medicine, Japan.
Katagiri-Kawade
M
M
Motoyoshi
F
F
Tashita
H
H
Teramoto
T
T
Kondo
N
N
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D017074:Common Variable Immunodeficiency / Q000201:enzymology*; D005260:Female; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D011493:Protein Kinase C / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007148:Immunoglobulin mu-Chains; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate