title: Effect of antiprogestin RU 486 on the proliferation and metabolic activity of stromal cells separated from human endometrium.
abstract: The effects of RU 486 on the proliferation and metabolic activity of human endometrial stromal cells in culture were studied. RU 486 at 10(-7) M/well significantly stimulated (P < 0.001) the growth as well as metabolic activity in the culture system. Interestingly, RU 486 at 10(-6) M/well did not stimulate metabolic activity in the culture. Progesterone, in combination with RU 486 at 10(-7) M/well, caused a significant increase in proliferation (assessed by thymidine incorporation) (P < 0.001) over control and P4 alone, but not significantly different from RU 486 at 10(-7) M/well alone. The same pattern was observed for metabolic functions (assessed by uridine incorporation) when RU 486 at 10(-7) M/well, along with P4, was added to the culture. Interestingly, RU 486 at 10(-6) M/well with P4 had no effect on RNA synthesis in the culture. The relevance of these findings is discussed.
authors.affiliation: Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi.
authors.forename: S
authors.initials: S
authors.lastname: Bhargava-Periwal
authors.forename: A
authors.initials: A
authors.lastname: Farooq
authors.forename: V L
authors.initials: VL
authors.lastname: Bhargava
authors.forename: N
authors.initials: N
authors.lastname: Bhatla
authors.forename: U
authors.initials: U
authors.lastname: Vij
authors.forename: K
authors.initials: K
authors.lastname: Murugesan
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D011374:Progesterone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006727:Hormone Antagonists; D015735:Mifepristone; D011374:Progesterone