In vitro and in vivo effects of activated macrophage supernatant on distal limb wounds of ponies.
OBJECTIVE
To determine whether monokines produced by activated rabbit peritoneal macrophages can inhibit development of exuberant granulation tissue formation in distal limb wounds in ponies.


METHODS
Randomized block.


METHODS
5 castrated male ponies, 2 to 6 years old and weighing 140 to 190 kg.


METHODS
In vitro activity of cell-free rabbit peritoneal macrophage supernatant was determined after incubation of fibroblasts from the flank and the distal portion of limbs of horses and ponies. Tritiated thymidine was then added, and after reincubation, radioactivity was measured. After creation of a 4-cm2, full-thickness wound on the mid dorsal aspect of each metacarpus and metatarsus of each pony, in vivo activity of the macrophage supernatant was evaluated. Biopsy specimens were collected at random sites near a border of each wound at 4, 6, and 10 weeks after creation of the wounds. Treatment effects were evaluated on the basis of presence of exuberant granulation tissue requiring excision, number of times that excision was required, total area of the wound, epithelialized area, area of granulation bed, and histologic evaluation of the biopsy specimens.


RESULTS
The macrophage supernatant effectively inhibited proliferation of equine fibroblasts in vitro. No significant in vivo treatment effects were found among the 4 treatment groups.


CONCLUSIONS
Monokines from stimulated rabbit peritoneal macrophages may have potential for improving wound healing in horses and ponies because of their effective inhibition in vitro of equine fibroblast proliferation.
Wilson
D A
DA
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
Adelstein
E H
EH
Keegan
K G
KG
Barrett
B A
BA
Kutz
R R
RR
D000704:Analysis of Variance; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005121:Extremities; D005347:Fibroblasts; D006734:Horse Diseases; D006736:Horses; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D015846:Monokines / Q000378:metabolism* / Q000627:therapeutic use*; D009919:Orchiectomy; D011817:Rabbits; D011897:Random Allocation; D014945:Wound Healing; D014947:Wounds and Injuries / Q000503:physiopathology* / Q000628:therapy / Q000662:veterinary*
D015846:Monokines