title: Gene structure of murine Gna11 and Gna15: tandemly duplicated Gq class G protein alpha subunit genes.
abstract: G protein alpha subunits are encoded by a multigene family of 16 genes that can be grouped into four classes, Gq, Gs, Gi, and G12. The Gq class is composed of four genes in mouse and human, and two of these genes, Gna11 and Gna15, cosegregate on mouse chromosome 10. We have characterized the gene structures of murine Gna11 and Gna15. The two genes are tandemly duplicated in a head-to-tail array. The upstream gene, Gna11, is ubiquitously expressed, whereas expression of the downstream gene, Gna15, is restricted to hematopoietic cells. The coding sequence of each gene is contained within seven exons, and the two genes together span 43 kb, separated by 6 kb of intergenic region. We have found no evidence for alternative splicing within the coding sequence of either gene. Sequence alignments show that the positions of the six intervening sequences are conserved in the two genes, consistent with Gna11 and Gna15 arising by tandem duplication from a common progenitor gene in vertebrates. Phylogenetic trees reveal unequal evolutionary rates among alpha subunits of the Gq class. The rate of change is approximately six fold higher in Gna15 than in Gna11.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas 75235-9041, USA.
authors.forename: I
authors.initials: I
authors.lastname: Davignon
authors.forename: M
authors.initials: M
authors.lastname: Barnard
authors.forename: O
authors.initials: O
authors.lastname: Gavrilova
authors.forename: K
authors.initials: K
authors.lastname: Sweet
authors.forename: T M
authors.initials: TM
authors.lastname: Wilkie
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D019204:GTP-Binding Proteins / Q000235:genetics*; D007438:Introns; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017421:Sequence Analysis
chemical_list: D017931:DNA Primers; D019204:GTP-Binding Proteins