Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.
We describe the synthesis of two new substrates for the detection of beta-galactosidase and evaluate their performance in comparison with that of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Of 171 Enterobacteriaceae strains that were able to hydrolyze X-Gal, 166 (97.1%) also hydrolyzed cyclohexenoesculetin-beta-D-galactoside whereas only 96 (56.1%) showed evidence of hydrolysis of 8-hydroxyquinoline-beta-D-galactoside. No false-positive results were observed with either substrate.
James
A L
AL
Department of Chemical and Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom.
Perry
J D
JD
Ford
M
M
Armstrong
L
L
Gould
F K
FK
D004755:Enterobacteriaceae / Q000201:enzymology; D005697:Galactosides / Q000138:chemical synthesis / Q000378:metabolism; D006868:Hydrolysis; D006912:Hydroxyquinolines / Q000138:chemical synthesis / Q000378:metabolism; D007202:Indicators and Reagents; D007211:Indoles / Q000378:metabolism; D013379:Substrate Specificity; D014468:Umbelliferones / Q000138:chemical synthesis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis*
C101994:8-hydroxyquinoline-beta-galactoside; D005697:Galactosides; D006912:Hydroxyquinolines; D007202:Indicators and Reagents; D007211:Indoles; D014468:Umbelliferones; C101993:cyclohexenoesculetin-beta-galactoside; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside