Isolation and partial characterization of cellulose-degrading strain of Streptomyces sp. LX from soil.
A new bacterium, Streptomyces sp. LX, was isolated from soil, which was aerobic Gram-positive and could decompose crystalline cellulose completely. Endo-cellulase with CMC-liquefying activity was detected when alpha-cellulose, Avicel, Whatman CF11 or CMC was used as carbon source, and its production varied with nature of the carbon source. Only traces of reducing sugar were found in cultures during incubation. This strain could produce FPase, beta-glucanase and short fibre generating activity. Exo- and endo-cellulase were detected in cultures by measuring formation of total sugar but were not detected by determining release of reducing sugar.
Li
X
X
Institute of Microbiology, Shandong University, Jinan, China.
Gao
P
P
D001673:Biodegradation, Environmental; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*
D002482:Cellulose; D002480:Cellulase