Isolation of a new epidermin variant from two strains of Staphylococcus epidermidis--frequency of lantibiotic production in coagulase-negative staphylococci.
A novel lantibiotic identified during the screening of 187 coagulase-negative staphylococci, has been structurally characterised. (Val1, Leu6)-epidermin was purified from culture supernatants of two Staphylococcus epidermidis strains, BN-V1 and BN-V301 following successive separation by adsorption, cation exchange and C18 reversed-phase chromatography. Separation of the purified peptides by SDS-polyacrylamide gel electrophoresis suggested a molecular mass of ca. 2000 Da and electrospray mass spectrometry subsequently demonstrated that both peptides had a mass of 2151 Da; a mass which is different from that of all previously described antimicrobial peptides. Amino acid analysis showed that both peptides contained lanthionine and were very similar to epidermin, while automated N-terminal sequencing by Edman degradation confirmed the identity of the two peptides as a natural variant of the antimicrobial lantibiotic epidermin, and demonstrated the conservative amino acid exchanges in positions one and six.
Israil
A M
AM
Institut Cantacuzino, Bucharest, Romania.
Jack
R W
RW
Jung
G
G
Sahl
H G
HG
D000409:Alanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001430:Bacteriocins; D003030:Coagulase; D014644:Genetic Variation; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000302:isolation & purification / Q000378:metabolism*; D013440:Sulfides
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D003030:Coagulase; D010455:Peptides; D013440:Sulfides; C050558:epidermin; C001520:lanthionine; D000409:Alanine