Identification and characterization of the porcine reproductive and respiratory virus ORFs 7, 5 and 4 products.
Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortions and respiratory diseases in pigs. The PRRSV genome is a positive-sense polyadenylated RNA molecule of about 15 kb. Along with the genes for structural proteins (envelope, matrix and nucleocapsid proteins) PRRSV genome contains a number of ORFs potentially encoding nonstructural proteins (ns). To investigate the nature of the PRRSV ORFs 7, 5 and 4 products, we have cloned the envelope (ORF5) nucleocapsid (ORF7) and ns4 (ORF4) protein genes in the bacterial expression vector pMAL(TM)-c2 under control of the "tac" promoter and expressed the proteins in E. coli. The recombinant proteins were recognized by porcine and rabbit hyperimmune serums to PRRSV, suggesting their structural nature.
Mounir
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Centre De Recherche En Virologie, Institut Armand-Frappier, Université du Québec, Laval, Canada.
Mardassi
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Dea
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D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D015153:Blotting, Western; D002139:Caliciviridae / Q000235:genetics* / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005260:Female; D008970:Molecular Weight; D016366:Open Reading Frames; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012140:Respiratory Tract Diseases / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000096:biosynthesis
D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins