title: Protein p60 participates in intestinal host invasion by Listeria monocytogenes.
abstract: The role of p60 in intestinal invasion by Listeria monocytogenes was assessed after oral infection of mice with the p60 low-expressing mutant RIII, or with anti-p60 antibody coated wild-type EGD. Invasion by L. monocytogenes RIII bacteria has been unimpaired suggesting that a low density of p60 suffices for entry. Up to 24 h post infection (p.i.), intestinal penetration by L. monocytogenes EGD bacteria was markedly reduced by coating with anti-p60 antibodies. In histological sections, anti-p60 antibody-treated L. monocytogenes EGD, but not uncoated listeriae were still detectable 24 h p.i. at the apical surface of enterocytes in the intestine. We conclude that p60 contributes to host invasion through the natural port of listerial entry, the intestinal epithelium.
authors.affiliation: Department of Immunology, University of Ulm, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hess
authors.forename: A
authors.initials: A
authors.lastname: Dreher
authors.forename: I
authors.initials: I
authors.lastname: Gentschev
authors.forename: W
authors.initials: W
authors.lastname: Goebel
authors.forename: C
authors.initials: C
authors.lastname: Ladel
authors.forename: D
authors.initials: D
authors.lastname: Miko
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D005260:Female; D007421:Intestine, Small / Q000382:microbiology* / Q000473:pathology; D007700:Kinetics; D008089:Listeria monocytogenes / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D008088:Listeriosis / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits
chemical_list: C078516:60 kDa protein, Listeria monocytogenes; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins