A physical map of the Q region of B1O.P.
The murine class I MHC Q region is part of a large complex multigene family whose members have various peptide binding functions. The structure of the Q region is complex, varying extensively in the b, d, k, and q haplotypes so far examined. To better understand the structural heterogeneity, we examined the Q region of B 10.P, a strain whose immunological characteristics are distinct from other haplotypes. A total of 89 cosmids were isolated from genomic DNA. The B 10.P Q region was found to contain seven genes in a 190-kb cluster linked to DP and two additional Q genes in a separate 55-kb cluster. The gene arrangement in this haplotype was unique and did not correspond to any other haplotype; this underscores the complexity of chromosomal structure in this region. In addition to the Q region clusters, Tla region was tentatively aligned in five clusters spanning approximately 300 kb. One 37-kb M region cosmid was also identified.
Litaker
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Program in Molecular Biology & Biotechnology, University of North Carolina, Chapel Hill 27599, USA.
Peace-Brewer
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Frelinger
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JA
D000818:Animals; D002874:Chromosome Mapping; D005805:Genes, MHC Class I / Q000235:genetics*; D006239:Haplotypes; D051379:Mice / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics