title: Cryopreservation of rooster semen in thirteen and sixteen percent glycerol.
abstract: Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 mu L; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb white Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4th d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period.
authors.affiliation: BioPore, Inc., State College, Pennsylvania 16805, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Gill
authors.forename: E G
authors.initials: EG
authors.lastname: Buss
authors.forename: R J
authors.initials: RJ
authors.lastname: Mallis
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005260:Female; D005298:Fertility / Q000502:physiology; D005990:Glycerol / Q000032:analysis / Q000494:pharmacology; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D012661:Semen / Q000187:drug effects / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology
chemical_list: D005990:Glycerol