Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.
Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.
Schena
M
M
Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
Shalon
D
D
Heller
R
R
Chai
A
A
Brown
P O
PO
Davis
R W
RW
D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D018869:Heat-Shock Response; D006801:Humans; D008832:Microchemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate