[Cloning of the diapause hormone gene of the silkworm (Bombyx mori)].
A silkworm genomic library was screened with radiolabelled cDNA of Bombyx mori diapause hormone (Bom DH). Three positive clones were isolated from approximately 4 x 10(5) plaques, and subjected to further analyses. The DNA inserts excised from positive clones with SalI were ligated into pBluescript KS(+) vector and grown in E. coli XL1 cells. The DNA fragments obtained by EcoRI digest form the insert were subcloned into the same vectors. The nucleotide sequence analysis showed that these clones involved the Bom DH gene. Genomic Southern analysis suggested that the Bom DH is encoded by a single gene. The present study which is the first one on cloning of the Bom DH gene.
Xu
W
W
Sericultural Research Institute, Chinese Academy of Agricultural Sciences Zhenjian.
Sato
Y
Y
Yamashita
O
O
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*
D009479:Neuropeptides; C075027:diapause hormone