Different toxic effects of hydrogen peroxide, nitric oxide, and superoxide on human, pig, and rat islets of Langerhans.
Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37 degrees C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.
Wacker
T
T
Third Medical Department, Justus-Liebig-University, Giessen, Germany.
Jahr
H
H
Weinand
S
S
Brandhorst
H
H
Brandhorst
D
D
Lau
D
D
Hering
B J
BJ
Federlin
K
K
Bretzel
R G
RG
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects*; D009569:Nitric Oxide / Q000633:toxicity*; D009599:Nitroprusside / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity*; D051381:Rats; D013045:Species Specificity; D013439:Sulfhydryl Reagents / Q000633:toxicity; D013481:Superoxides / Q000633:toxicity*; D013552:Swine; D014969:Xanthine Oxidase / Q000633:toxicity
D016877:Oxidants; D013439:Sulfhydryl Reagents; D013481:Superoxides; D009599:Nitroprusside; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase