title: Spontaneously hypertensive rats are resistant to the development of hypercholesterolemia.
abstract: The arterioles of young spontaneously hypertensive rats (SHR) are purported to have an enhanced sensitivity to nitric oxide (NO)-dependent vasodilators, relative to normotensive animals, while NO-related arteriolar responses are diminished in both mature SHR as well as hypercholesterolemic normotensive rats. Because endothelial production of NO relaxes vascular smooth muscle and inhibits platelet adhesion and aggregation, hypercholesterolemia may synergistically affect the development of genetic hypertension. The NO-mediated baseline vascular tone, acetylcholine-induced dilation, and inhibition of platelet thrombus formation were studied over time (10 weeks) in SHR and hypercholesterolemic SHR (HC-SHR). The in vivo microcirculation of the cremaster muscle was used to quantitate all observations. The HC-SHR became significantly hypercholesterolemic after 1 week on the cholesterol-supplemented diet, with serum cholesterol concentrations remaining elevated for the 10 weeks studied. However, the serum cholesterol concentrations of HC-SHR were significantly less than those of Sprague-Dawley and Wistar-Kyoto rats fed the same diet. Dietary hypercholesterolemia did not exacerbate the development of genetic hypertension. Second- and third-order arterioles of SHR and age-matched HC-SHR constricted to the same extent when the NO synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME) was applied. The third order arterioles of both groups also dilated the same amount to acetylcholine and sodium nitroprusside. Platelet thrombus formation induced by light/dye photochemistry was not different between the SHR and HC-SHR groups either at 1 or 10 weeks of diet, and L-NAME decreased the time to thrombus occlusion of blood flow equally in both groups. This is in marked contrast to the previously reported hypercholesterolemia-induced decreases in vascular reactivity in Sprague-Dawley rats. These current findings demonstrate that SHR are resistant to the development of hypercholesterolemia and that NO-mediated vascular responses in SHR are not attenuated by hypercholesterolemia.
authors.affiliation: Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Lindberg
authors.forename: D A
authors.initials: DA
authors.lastname: Schuschke
authors.forename: F N
authors.initials: FN
authors.lastname: Miller
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects / Q000528:radiation effects; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004198:Disease Susceptibility; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D006339:Heart Rate / Q000187:drug effects; D006937:Hypercholesterolemia / Q000209:etiology*; D006973:Hypertension / Q000150:complications* / Q000235:genetics; D008027:Light; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine; D014664:Vasodilation
chemical_list: D002791:Cholesterol, Dietary; C039217:fluorescein isothiocyanate bovine serum albumin; D009599:Nitroprusside; D012710:Serum Albumin, Bovine; D001120:Arginine; D019001:Nitric Oxide Synthase; D016650:Fluorescein-5-isothiocyanate; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester