Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map.
Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.
Nechiporuk
T
T
Division of Neurology, University of California at Los Angeles 90048, USA.
Nechiporuk
A
A
Guan
X
X
Frederick
R
R
Figueroa
K
K
Chumakov
I
I
Korenberg
J R
JR
de Jong
P J
PJ
Pulst
S M
SM
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D013132:Spinocerebellar Degenerations / Q000235:genetics*
D004276:DNA, Satellite