Expression pattern of c-sis, c-fos and c-jun in human placenta and embryofetal organs.
The expression pattern of c-sis, c-fos and c-jun was investigated in placenta and embryofetal organ specimens from the first trimester. Northern analysis of the placentae showed c-sis transcripts and c-fos expression. Northern analysis of the same genes in embryofetal organs pointed to the brain as the only organ where consistent transcriptional activity could be observed. RT-PCR analysis of c-fos and c-jun in placentae, staged at four different time periods in pregnancy, allowed to detect the expected fragments in all cases. The same was true when c-fos and c-jun were analyzed at the 13th week of gestation in all the embryofetal organs.
Rossi
D
D
Department of Biology and Genetics, Medical Faculty, University of Milan, Italy.
Del Giacco
L
L
Doneda
L
L
Nicolini
U
U
Acaia
B
B
Brioschi
D
D
Larizza
L
L
D015152:Blotting, Northern; D017931:DNA Primers; D005260:Female; D005333:Fetus / Q000378:metabolism*; D015870:Gene Expression; D016762:Genes, fos / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics*; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase
D017931:DNA Primers; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase