Fumonisin B1 toxicity in male Sprague-Dawley rats.
Male rats were gavaged with fumonisin B1 (FB1) once daily for 11 consecutive days at doses of 0, 1, 5, 15, 35, and 75 mg FB1/kg body weight. Urine osmolality (at 5-75 mg FB1/kg) and organic ion transport in kidney slices (at 5-75 mg FB1/kg) were reduced. Urinary excretion of protein (at 15-75 mg FB1/kg) and of the enzymes LDH (at 5-75 mg FB1/kg), NAG (at 5-75 mg FB1/kg) and GGT (at 15-75 mg FB1/kg) were increased. These findings were indicative of glomerular and tubular toxicity. Histopathologic changes in the kidney consisted of necrosis of tubular epithelia of variable extent accentuated in the inner cortex. These changes were present at 1 and 5 mg FB1/kg and were more pronounced at 15-75 mg FB1/kg. Serum enzymes indicative of hepatotoxicity (ALT, GGT) were elevated compared to controls at 75 mg FB1/kg only. There were noticeable increases in mitotic figures in hepatocytes at 35-75 mg FB1/kg, while single cell necroses were increasingly numerous from 15-75 mg FB1/kg. The kidneys were considered to be the primary target organs in this study.
Bondy
G
G
Toxicology Research Division, Food Directorate, Health Canada, Ottawa.
Barker
M
M
Mueller
R
R
Fernie
S
S
Miller
J D
JD
Armstrong
C
C
Hierlihy
S L
SL
Rowsell
P
P
Suzuki
C
C
D000118:Acetylglucosaminidase / Q000652:urine; D000818:Animals; D003404:Creatinine / Q000097:blood / Q000652:urine; D037341:Fumonisins; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000652:urine; D007958:Leukocyte Count; D008099:Liver / Q000473:pathology; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D009994:Osmolar Concentration; D011507:Proteinuria; D051381:Rats; D017207:Rats, Sprague-Dawley; D000637:Transaminases / Q000097:blood; D014556:Urine; D015430:Weight Gain; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000652:urine
D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000637:Transaminases; D000118:Acetylglucosaminidase