Simultaneous determination of phenol, cresol, xylenol isomers and naphthols in urine by capillary gas chromatography.
An attempt was made to establish a method for the simultaneous determination of urinary concentrations of phenol, o-, p- and m-cresols, 1- and 2-naphthol and xylenol isomers by capillary gas chromatography. Urine samples were extracted after acid hydrolysis of glucuronides and sulfates by solid-phase extraction. The ten substances were separated gas chromatographically using a capillary column (Ultra 2) of cross-linked 5% phenylmethyl silicone. Calibration graphs were linear for 5-100 micrograms/ml of all the phenols determined. The corresponding detection limits for phenolic compounds varied from 0.1 to 0.2 microgram/ml. The relative standard deviations for samples in urine were in the range 2.6 - 16.6% and the accuracy was in the range 1.4-25%. Recoveries were generally over 80%.
Bieniek
G
G
Department of Biochemistry and Biophysics, Silesian Medical Academy, Sosnowiec, Poland.
D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods; D003408:Cresols / Q000737:chemistry / Q000652:urine*; D006801:Humans; D016014:Linear Models; D009284:Naphthols / Q000737:chemistry / Q000652:urine*; D009784:Occupational Diseases / Q000652:urine; D016273:Occupational Exposure; D019800:Phenol; D010636:Phenols / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013565:Sympatholytics / Q000737:chemistry / Q000652:urine*; D014992:Xylenes / Q000737:chemistry / Q000652:urine*
D003408:Cresols; D009284:Naphthols; D010636:Phenols; D013565:Sympatholytics; D014992:Xylenes; D019800:Phenol; C016834:3,5-xylenol