Laminin stimulates expression of two mitochondrial proteins during neurite outgrowth.
Differential hybridization was utilized with mRNA from NG108-15 cells cultured on either tissue culture plastic or laminin for 4 hr to identify genes whose mRNA was increased by laminin, a potent stimulator of neurite outgrowth. Two of the 16 laminin-induced clones, cytochrome b and chargerin II, are mitochondrial proteins. Northern blotting confirmed that laminin increased the mRNA levels of cytochrome b and chargerin II several fold. Antibody to chargerin II stained both processes and cell bodies of the cerebellar Purkinje cells and localized in the mitochondria of NG108-15 cells, which also showed increased protein levels in the presence of laminin. In addition, higher levels of chargerin II protein were detected in the newborn brain compared to the adult. However, inhibitors of mitochondrial protein synthesis did not affect laminin-mediated neurite outgrowth. These data suggest that the increased synthesis of mitochondrial enzymes observed with laminin treatment may not be necessary for the formation of neurites.
Weeks
B S
BS
University of Pennsylvania, Department of Medicine, Philadelphia, PA 19104, USA.
Burbelo
P
P
Jucker
M
M
Weiner
M A
MA
Roque
E
E
Kleinman
H K
HK
D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D019085:Fluorescent Antibody Technique, Direct; D007150:Immunohistochemistry; D007797:Laminin / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D007797:Laminin; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D012333:RNA, Messenger; C044593:chargerins; D004247:DNA