A physical and genetic map of Neisseria meningitidis B1940.
A physical map of the chromosome of Neisseria meningitidis B1940 has been constructed by one- and two-dimensional pulsed-field gel electrophoresis techniques. Complete macrorestriction maps for the enzymes Nhel (16 sites), Sgfl (13 sites), Sfil (11 sites) and l-Ceul (4 sites), as well as a partial restriction map for the restriction enzyme Spel (15 of c. 28 sites) could be established. Altogether, 59 restriction sites were mapped on a single circular chromosome of 2.3 Mbp. By restriction endonuclease digestion and Southern hybridization of cloned genetic markers, 39 genetic loci were assigned to this map. Comparison with the metabolic maps of Neisseria gonorrhoeae MS11-N198 and FA1090 revealed a high degree of conservation in the arrangement of gene loci among these two species, although four out of 24 genetic loci are located at different chromosomal positions, indicating several genomic rearrangements.
Gäher
M
M
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
Einsiedler
K
K
Crass
T
T
Bautsch
W
W
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016680:Genome, Bacterial; D009345:Neisseria meningitidis / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping
D004262:DNA Restriction Enzymes