title: Bacteriocin release proteins: mode of action, structure, and biotechnological application.
abstract: The mechanism by which Gram-negative bacteria like Escherichia coli secrete bacteriocins into the culture medium is unique and quite different from the mechanism by which other proteins are translocated across the two bacterial membranes, namely through the known branches of the general secretory pathway. The release of bacteriocins requires the expression and activity of a so-called bacteriocin release protein and the presence of the detergent-resistant phospholipase A in the outer membrane. The bacteriocin release proteins are highly expressed small lipoproteins which are synthesized with a signal peptide that remains stable and which accumulates in the cytoplasmic membrane after cleavage. The combined action of these stable, accumulated signal peptides, the lipid-modified mature bacteriocin release proteins (BRPs) and phospholipase A cause the release of bacteriocins. The structure and mode of action of these BRPs as well as their application in the release of heterologous proteins by E. coli is described in this review.
authors.affiliation: Department of Molecular Microbiology, IMBW, BioCentrum Amsterdam, Faculty of Biology, The Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: van der Wal
authors.forename: J
authors.initials: J
authors.lastname: Luirink
authors.forename: B
authors.initials: B
authors.lastname: Oudega
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001430:Bacteriocins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000502:physiology; D021382:Protein Sorting Signals / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; D021382:Protein Sorting Signals; C052368:cloacin DF13-encoded bacteriocin release protein, E coli; D010741:Phospholipases A