Cytophotometric DNA-analysis of bone-tumors. A new method for preparing formalin-fixed tissue and the regarding of DNA-malignancy grade and its prognostic value.
78 benign and malign, formalin-fixed bone or bone-associated tumors of different origin were prepared for image-cytophotometric measuring with a new method of preparing. The DNA-content of the tumors and the so resulting malignancy-grade were determined and compared with the corresponding measurements of unprepared fresh tumors respectively paraffin-embedded tissue sections. Regarding the comparison of the fresh material with the formalin-fixed material, no significant difference (p < or = 0.01) of the data could be found. There was also no significant difference (p < or = 0.01) concerning the comparison of the data obtained from the formalin-fixed material and the data from the tissue sections. The criterium aneuploidy showed a sensitivity of 86% and a specificity of 100% in regards to identifying a malignant tumor, irrespective of the preparation method. A strong correlation could be found (p < 0.001) between the DNA-malignancy grade and the survival rate of the patients.
Adler
C P
CP
Institute of Pathology, University of Freiburg, Germany.
Neuburger
M
M
Herget
G W
GW
Klemm
C E
CE
Saur
S
S
D000367:Age Factors; D000782:Aneuploidy; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D003592:Cytophotometry / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D008297:Male; D011379:Prognosis; D016707:Tissue Fixation / Q000379:methods
D004273:DNA, Neoplasm; D005557:Formaldehyde