title: Urinary immunoreactive brain natriuretic peptide in patients with renal disease.
abstract: Urinary immunoreactive brain natriuretic peptide (BNP) was studied by radioimmunoassay in patients with renal disease. Urinary immunoreactive human BNP excretion measured in 11 normal subjects was 3.82 +/- 0.62 pmol/day (mean +/- SEM). Significantly increased 24-h urinary secretion of immunoreactive human BNP was noted in patients with chronic renal failure (11.07 +/- 1.73 pmol/day, n = 9, P < 0.05 to normal subjects). A significant correlation was noted between 24-h urinary excretion of immunoreactive human BNP and creatinine clearance in patients with various renal diseases (r = -0.43, P < 0.01, n = 45). Gel chromatography of the urine extracts obtained from normal subjects and patients with chronic renal failure showed multiple immunoreactive peaks; two eluting earlier, one in the position of human BNP-32 and others eluting later. Reverse-phase high-performance liquid chromatography of the urine extracts showed a peak in the position of human BNP-32 and a peak eluting earlier. These findings indicate that: (1) immunoreactive human BNP is present in human urine; (2) urinary immunoreactive human BNP consists of multiple components, i.e., human BNP-32 itself or a substance very similar to it, smaller molecular forms which are probably metabolic products of human BNP-32, and larger molecular forms; and (3) 24-h urinary excretion of immunoreactive human BNP is increased in patients with renal dysfunction.
authors.affiliation: Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai 980-77, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Totsune
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: F
authors.initials: F
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Sone
authors.forename: M
authors.initials: M
authors.lastname: Ohneda
authors.forename: C
authors.initials: C
authors.lastname: Satoh
authors.forename: O
authors.initials: O
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Mouri
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003240:Connective Tissue Diseases / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D003920:Diabetes Mellitus / Q000378:metabolism; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate / Q000502:physiology; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000378:metabolism / Q000652:urine*; D011863:Radioimmunoassay
chemical_list: D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; C076910:brain natriuretic peptide, porcine; D003404:Creatinine