title: Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: I. thymus.
abstract: Thymi of adult chickens (White Leghorn strain), ages of 6, 18, 45, days and 1, 3, 6, months, were studied by histological and histochemical stainings, histoenzymatic reactions (LDH, SDH, alpha-GPDH, NADH, NADPH, Ca++ dependent ATPase, pH 8.5) and by anti-thymostimulin immunoreaction. Positive reactions for thymostimulin-like substance, mucopolysaccharides and enzymatic activities were located in different epithelial compartments of the cortical and medullary zones. In 3 and 6 month-old chickens, there was a progressive reduction of the cortical area and of the number of cortical and medullary epithelial cells, and, in the thymi of the 6 month-old chickens, a partial decline in the immunological reactivity to anti-TS, but the intensity of the histochemical and enzymatic reactivities, in comparison with the thymi of the youngest chickens, was not decreased. This may suggest that the cortical and medullary epithelial cells still present in these thymi are capable of secreting and expressing activities and, thus, may maintain their functions.
authors.affiliation: Institute of Human Physiology, University "La Sapienza", Roma, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Aita
authors.forename: A M
authors.initials: AM
authors.lastname: Mazzone
authors.forename: F
authors.initials: F
authors.lastname: Gabrielli
authors.forename: A
authors.initials: A
authors.lastname: Evangelista
authors.forename: S
authors.initials: S
authors.lastname: Brenna
mesh_terms: D000276:Adjuvants, Immunologic / Q000096:biosynthesis*; D000375:Aging / Q000502:physiology*; D000818:Animals; D002645:Chickens; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008208:Lymphatic System / Q000502:physiology*; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis; D013949:Thymus Extracts / Q000096:biosynthesis*; D013950:Thymus Gland / Q000166:cytology* / Q000201:enzymology / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D013949:Thymus Extracts; C031407:thymostimulin; D009243:NAD; D009249:NADP; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase