High-performance liquid chromatography of alpha-amino acids and aztreonam on reversed phase columns with aqueous Cu2+ as eluent.
Parameters affecting the separation of amino acids on different RP-HPLC columns were studied. Six amino acids were separated on Zorbax TMS, Zorbax CN, Zorbax ODS and Zorbax C8, using 1.8 x 10(-3) M copper sulphate at pH 4.1 as aqueous mobile phase. The best separation was shown by Zorbax TMS followed by Zorbax CN. The column performance was maintained by serial washing after 6 h continuous work with aqueous 10(-4) M disodium edetate, 10(-4) N sulphuric acid, water and gradient elution with aqueous methanol. The separation mechanism was interpreted. The method was applied for separation and quantification of aztreonam and L-arginine in Azactam vials.
Khedr
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Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Assiut University, Egypt.
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D001120:Arginine / Q000032:analysis* / Q000737:chemistry; D001398:Aztreonam / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019327:Copper Sulfate / Q000737:chemistry*; D018698:Glutamic Acid / Q000032:analysis / Q000737:chemistry; D006639:Histidine / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008997:Monobactams / Q000032:analysis* / Q000737:chemistry; D009952:Ornithine / Q000032:analysis / Q000737:chemistry; D010649:Phenylalanine / Q000032:analysis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013912:Threonine / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D008997:Monobactams; D014867:Water; D013912:Threonine; D018698:Glutamic Acid; D010649:Phenylalanine; D006639:Histidine; D001120:Arginine; D009952:Ornithine; D001398:Aztreonam; D019327:Copper Sulfate