Use of salivary caffeine tests to assess the inducer effect of a drug on hepatic metabolism.
OBJECTIVE
To validate the use of successive salivary caffeine tests in evaluating how long inducer drugs affect hepatic metabolism. The time course of the inducer effect of rifampin found in other studies using different methodologies was chosen as the time course of reference.


METHODS
Open-label, prospective, longitudinal study.


METHODS
A university hospital.


METHODS
Five healthy volunteers.


METHODS
Rifampin 600 mg/d was administered for 21 days. Anhydrous caffeine 300 mg was concurrently administered on each study day. Salivary caffeine tests were carried out on the following days: predose (baseline), and days 1, 5, 9, 13, and 17. Salivary tests were performed for up to 13 days after the last dose of rifampin (study days 21, 25, 29, and 33).


RESULTS
The mean systemic caffeine clearance was increased for up to 17 days after the intake of rifampin, reaching the maximum inducer effect between days 5 and 9, and returning to previous values progressively during several days after rifampin was discontinued.


CONCLUSIONS
Our results suggest that successive salivary caffeine measurements could be a safe, reliable, noninvasive, and suitable test for exploring the time course of the inducer effect of drugs on hepatic metabolism activity.
Soto
J
J
Clinical Pharmacology Unit, Hospital Santa Cruz, (Cantabria), Spain.
Sacristan
J A
JA
Alsar
M J
MJ
D000328:Adult; D002110:Caffeine / Q000032:analysis / Q000493:pharmacokinetics*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010865:Pilot Projects; D011446:Prospective Studies; D012293:Rifampin / Q000494:pharmacology*; D012463:Saliva / Q000737:chemistry*
D002110:Caffeine; D012293:Rifampin