title: In vitro site-directed mutagenesis using the unique restriction site elimination (USE) method.
authors.affiliation: Clontech Laboratories, Palo Alto, CA, USA.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D017384:Sequence Deletion; D014169:Transformation, Bacterial
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes