Characterization of a new gene that encodes a functional MHV receptor and progress in the identification of the virus-binding site(s).
Several splice variants of the murine biliary glycoprotein 1 (Bgp 1) gene in the carcinoembryonic antigen gene superfamily serve as cellular receptors for mouse hepatitis virus. RNAPCR and immunoblot analysis of the receptor in inbred mouse strains showed that the glycoproteins expressed in SJL/J mice are encoded by an allelic variant of the Bgp 1 gene, named Bgp 1b. We recently cloned and characterized a second gene, Bgp 2, that encodes a functional MHV receptor glycoprotein which is not recognized by anti-MHVR MAb-CC1. A third gene related to Bgp 1 was cloned and expressed and shown to encode a soluble protein called Cea-10 that differs significantly in its N-terminal domain from Bgp 1 and Bgp 2. Chimeric proteins constructed between the different murine Bgps and point mutations in the prototype MHV receptor, Bgp 1a or MHVR, were analyzed to further characterize the MAb-CC1-binding and virus-binding domains within the N terminal domain of the receptor. Thus, the murine host for MHV expresses multiple splice variants of mRNAs encoded by several different Bgp-related genes which differ in their ability to serve as MHV receptors. The differential expression of these genes in different murine tissues may help to explain the tissue tropism of MHV strains.
Dveksler
G
G
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
Nedellec
P
P
Lu
J H
JH
Keck
U
U
Basile
A
A
Cardellichio
C
C
Zimmermann
W
W
Beauchemin
N
N
Holmes
K V
KV
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D014644:Genetic Variation; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000502:physiology*; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; C069847:CD66 antigens; C087701:Ceacam2 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins