title: Effects of basophil-priming and stimulating cytokines on histamine release from isolated human skin mast cells.
abstract: Cell priming and stimulation of different cytokines (which include chemokines and growth factors) are typical features of human basophils. Recently, it has been shown that the macrophage chemotactic protein-1 (MCP-1), RANTES and macrophage inflammatory protein-1 alpha (MIP-1 alpha) are potent direct secretagogues for human basophils and that interleukin-3 (IL-3), IL-5 and granulocyte/macrophage colony-stimulating factor (GM-CSF) are priming factors for subsequent potentiation of mediator release from basophils induced by different stimuli. This observation may be clinically important for the activation and recruitment of inflammatory cells in different immune responses of the skin (e.g. late-phase reactions). The aim of the present study was to investigate whether cytokines and chemokines are also capable of priming or stimulating isolated human skin mast cells (SMC). SMC were either stimulated directly with the cytokines alone or preincubated with these factors for 10 min before being activated with suboptimal concentrations of anti-IgE, A23187 or substance P. IL-3, IL-5, GM-CSF, platelet factor-4 (PF-4), IL-8, MCP-1 and MIP-1 alpha (each at concentrations of 1 ng/ml to 1 microgram/ml, log steps) did not significantly modulate histamine release from SMC induced by the three different secretagogues. RANTES exhibited a weak but significant potentiating effect on IgE-mediated activation. Stem cell factor (SCF) as a positive control was able to prime mast cell histamine release strongly. In addition, PF-4, MCP-1, RANTES and MIP-1 alpha were incapable of inducing direct histamine release from SMC. In experiments with isolated human peripheral basophils, however, we observed potent Fc epsilon RI-mediated priming effects evoked through IL-3, IL-5 and GM-CSF. We conclude that SMC derived from healthy donors are not targets of (immuno)modulatory factors that prime or stimulate basophils.
authors.affiliation: Department of Dermatology, Medical University of Lübeck, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Nitschke
authors.forename: K
authors.initials: K
authors.lastname: Sohn
authors.forename: D
authors.initials: D
authors.lastname: Dieckmann
authors.forename: B F
authors.initials: BF
authors.lastname: Gibbs
authors.forename: H H
authors.initials: HH
authors.lastname: Wolff
authors.forename: U
authors.initials: U
authors.lastname: Amon
mesh_terms: D001491:Basophils / Q000502:physiology*; D018932:Chemokine CCL2 / Q000494:pharmacology; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D019402:Macrophage Inflammatory Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor