Potentiometric alternating biosensing system for in continuum toxicity tests on cell population.
In this paper, we investigate the possibility to monitor, in continuum, changes of cellular metabolism. Examples of this feature are shown: metabolic changes due to drugs administration are evaluated as a decrease of extracellular acidification rate on CHO-K1 and HeLa cells. Parallel experiments with a standard test were performed to validate the results obtained with the biosensor.
Adami
M
M
Technobiochip, Società di Ricerca Industriale Avanzata, Marciana (LI), Italy.
Zolfino
I
I
Fenu
S
S
Nardelli
D
D
Nicolini
C
C
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D015374:Biosensing Techniques; D016466:CHO Cells / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D006224:Cricetinae; D004594:Electrophysiology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008727:Methotrexate / Q000494:pharmacology; D011199:Potentiometry / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives / Q000494:pharmacology
D000970:Antineoplastic Agents; C001943:5-fluorouridine; D002945:Cisplatin; D014529:Uridine; D008727:Methotrexate