title: Regulation of NAD+ glycohydrolase activity by NAD(+)-dependent auto-ADP-ribosylation.
abstract: NAD+ glycohydrolase (NADase; EC 3.2.2.5) is an enzyme that catalyses hydrolysis of NAD+ to produce ADP-ribose and nicotinamide. Its physiological role and the regulation of its enzymic activity have not been fully elucidated. In the present study, the mechanism of self-inactivation of NADase by its substrate, NAD+, was investigated by using intact rabbit erythrocytes and purified NADase. Our results suggest that inactivation of NADase was due an auto-ADP-ribosylation reaction. ADP-ribosylated NADase of rabbit erythrocytes was deADP-ribosylated when incubated without NAD+, and thus enzyme activity was simultaneously restored. These findings suggest that reversible auto-ADP-ribosylation of NADase might regulate the enzyme's activity in vivo.
authors.affiliation: Department of Biochemistry, Chonbuk National University Medical School, Chonju, Korea.
authors.forename: M K
authors.initials: MK
authors.lastname: Han
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Cho
authors.forename: Y M
authors.initials: YM
authors.lastname: Song
authors.forename: N H
authors.initials: NH
authors.lastname: An
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.forename: U H
authors.initials: UH
authors.lastname: Kim
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000737:chemistry; D004912:Erythrocytes / Q000201:enzymology; D066298:In Vitro Techniques; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism*; D009244:NAD+ Nucleosidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits
chemical_list: D009243:NAD; D000246:Adenosine Diphosphate Ribose; D009244:NAD+ Nucleosidase; D003545:Cysteine