title: Study the presence of fibronectin binding protein (FnBp) in tuberculosis (TB) patients by elisa.
abstract: Fibronectin-binding protein (FnBp) antigens are a prominent secretory protein of short term culture supernatants of M. tuberculosis and M. bovis (BCG) and is conserved within the genus Mycobacterium. The 30/31 kDa antigen of M. tuberculosis is one of the major secretory molecules and is probably routinely recognised by the host immune system in the early stage of tuberculosis infection. Serum immune complexes, prepared from TB patients and normals, were analysed for the presence of FnBp by ELISA using an anti-30/31 kDa (FnBp) monoclonal antibody (CF8) and by western blotting using Fibronectin-HRP. A significant difference was seen between normals and TB patients (p < 0.05). This test was found to have a specificity of 80% and a positive predictive value of 73%. This is a preliminary finding and the test needs to be evaluated further for its performance on a larger number of confirmed TB patients and controls.
authors.affiliation: Department of Microbiology, University of Karachi, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Ahmad
authors.forename: S
authors.initials: S
authors.lastname: Afghan
authors.forename: C
authors.initials: C
authors.lastname: Raykundalia
authors.forename: D
authors.initials: D
authors.lastname: Catty
mesh_terms: D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins; D015153:Blotting, Western; D002352:Carrier Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C078635:fibronectin-binding proteins, bacterial