title: Localization of GABAA alpha 1 mRNA subunit in the brainstem nuclei controlling esophageal peristalsis.
abstract: The nucleus of the solitary tract, the site of esophageal premotor neurons (PMN), is tonically inhibited by GABAergic neurons via the GABAA receptor. We investigated the expression of GABAA alpha 1 subunit mRNA within esophageal PMNs of the NTS utilizing transynaptic tracing with pseudorabies virus and nonisotopic in-situ hybridization. Double-labeling studies revealed that the majority of PRV-immunoreactive cells also expressed GABAA alpha 1 mRNA. The expression of GABAA subunits supports a role for GABA in the brainstem circuit controlling esophageal peristalsis.
authors.affiliation: Division of Gastrenterology and Nutrition, Children's Hospital of Philadelphia, PA 19104, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Broussard
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S M
authors.initials: SM
authors.lastname: Altschuler
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism / Q000502:physiology*; D004947:Esophagus / Q000502:physiology*; D046911:Macromolecular Substances; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010528:Peristalsis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000096:biosynthesis*; D017552:Solitary Nucleus / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D011963:Receptors, GABA-A