Rapid in situ harvesting and cytogenetic analysis of perinatal tissue samples.
Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low O2 conditions, pre-harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8.7 days (range 2-17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis.
Fisher
A M
AM
Wessex Regional Genetics Laboratory, Salisbury District Hospital, Wiltshire, U.K.
Cockwell
A E
AE
Moore
K J
KJ
Gregson
N M
NM
Campbell
P L
PL
Campbell
C M
CM
Herbert
A
A
Barber
J C
JC
Crolla
J A
JA
D000650:Amnion / Q000473:pathology; D001706:Biopsy; D002871:Chromosome Banding / Q000379:methods*; D017364:Collagenases / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D016591:Histocytological Preparation Techniques; D006801:Humans; D009132:Muscles / Q000196:embryology / Q000473:pathology; D010053:Ovary / Q000196:embryology / Q000473:pathology; D010865:Pilot Projects; D010920:Placenta / Q000473:pathology; D011247:Pregnancy; D012867:Skin / Q000196:embryology / Q000473:pathology; D013997:Time Factors; D014470:Umbilical Cord / Q000473:pathology
D017364:Collagenases