Contribution of the glycolytic pathway to enzymatic metmyoglobin reduction in myocytes.
The metmyoglobin (oxidized form of myoglobin) reducing enzyme system was examined in rat myocytes. An inhibitor of the glycolytic pathway (2-deoxy-D-glucose) inhibited the reduction of metmyoglobin in myocytes. An inhibitor of the citric acid cycle (malonic acid), did not inhibit this reaction. From the results of this study along with those of our previous work, we proposed that the glycolytic pathway may have a significant role in the enzymatic metmyoglobin reduction, presumably by its supply of cytoplasmic NADH for the NADH-cytochrome b5 reductase system, in muscle.
Arihara
K
K
School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada-shi, Japan.
Itoh
M
M
Kondo
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003579:Cytochrome Reductases / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase; D003847:Deoxyglucose / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D008314:Malonates / Q000494:pharmacology; D008786:Metmyoglobin / Q000378:metabolism*; D008956:Models, Chemical; D009206:Myocardium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012977:Sodium Nitrite / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D008314:Malonates; D008786:Metmyoglobin; D003847:Deoxyglucose; C030290:malonic acid; D013385:Succinate Dehydrogenase; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D012977:Sodium Nitrite