Effects of an upstream DNA curvature on the strength of HSV thymidine kinase promoter.
The effect of an upstream DNA curvature on the strength of the herpes simplex virus thymidine kinase (TK) promoter was investigated by employing luciferase assay. A set of double-stranded oligonucleotides, each with an unique DNA configuration, were synthesized and substituted into an upstream region of the promoter. It was revealed from the assay that both plane and space curves exerted a negative influence upon the strength of the promoter.
Ohyama
T
T
Department of Biology, Faculty of Science, Konan University, Kobe, Japan.
Kadokawa
Y
Y
Hirota
Y
Y
D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D013937:Thymidine Kinase