Interaction of oligonucleotides with a single stranded DNA binding site of RecA protein.
Model peptide Ile-Arg-Met-Lys-Ile-Gly-Val-Met-Phe-Gly-Asn-Pro-Glu-Thr- Thr-Thr-Gly-Gly-Asn-Ala-Leu-Lys-Phe-Tyr for a single stranded DNA (ssDNA) binding site of RecA protein has been synthesized and its interaction with some ssDNAs and ssRNAs has been investigated. Circular dichroism spectra showed that conformational change of the peptide by the addition of a ssDNA dACCTAGTC was much faster than that by the addition of a ssRNA rACCUAGUC. The addition of a neutral DNA or a 2'-5' linked oligonucleotide did not lead to the conformational change of the peptide.
Nakano
S
S
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Sasaki
M
M
Sugimoto
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D010446:Peptide Fragments; D012313:RNA; D011938:Rec A Recombinases