Reduced platelet glutathione peroxidase activity and serum selenium concentration in atopic asthmatic patients.
BACKGROUND
Asthmatic inflammation results in increased oxygen free radical generation and assessment of the activity of the selenium (Se) dependent anti-oxidant enzyme, glutathione peroxidase (GSH-Px) in asthma may therefore be important.


OBJECTIVE
To test the hypothesis that reduced GSH-Px activity and Se intake contribute to asthmatic inflammation, platelet and whole blood GSH-Px activities and serum and whole blood Se concentrations were measured and compared in atopic and non-atopic asthmatic patients and non-asthmatic control subjects.


METHODS
GSH-Px activities of whole blood and isolated platelets were assessed in 41 asthmatic patients (33 atopic) and 41 age- and sex-matched non-asthmatic subjects (15 atopic) by spectrophotometric assay based on the oxidation of NADPH. Se concentrations were determined by semi-automated fluorimetric assay.


RESULTS
Mean (+/-SD) platelet GSH-Px activity was lower in asthmatic (89.5 +/- 45.7 mumol NADPH oxidized min-1 g-1 of protein) than in non-asthmatic subjects (109.9 +/- 41.9; P = 0.038) and in atopic (89.7 +/- 45.1, n = 48) compared with non-atopic subjects (113.7 +/- 40.9, n = 34; P = 0.016). Mean whole blood GSH-Px activity was also lower in atopic (12.2 +/- 5.2 mumol NADPH oxidized min-1 g-1 of Hb) than in non-atopic subjects (14.5 +/- 4.2; P = 0.038). In non-asthmatic subjects, the mean whole blood GSH-Px activity was lower in men (9.9 +/- 3.5) than in women (14.5 +/- 3.7; P = 0.0004) and was positively correlated with age (r = 0.51; P = 0.0006). Mean serum Se was lower in asthmatic (1.07 +/- 0.12 mumol/L) than in non-asthmatic subjects (1.16 +/- 0.31; P = 0.036). Using multiple linear regression, asthma was an independent predictor of decreased platelet GSH-Px after gender, age and serum Se were taken into account (P = 0.048) while atopy was a significant predictor of low whole blood GSH-Px independent of asthma, gender, age and whole blood Se (P = 0.033).


CONCLUSIONS
In addition to Se status, atopy, gender and age all appear to influence GSH-Px activity, although the relative importance of these factors may differ in asthmatic and non-asthmatic populations. It seems likely that the reduced activity of this enzyme in platelets and blood may reflect mechanisms associated with the pathogenesis and severity of asthma.
Misso
N L
NL
Department of Medicine, University of Western Australia, Queen Elizabeth II Medical Centre, Perth, Australia.
Powers
K A
KA
Gillon
R L
RL
Stewart
G A
GA
Thompson
P J
PJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D001249:Asthma / Q000097:blood*; D001792:Blood Platelets / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D012044:Regression Analysis; D012643:Selenium / Q000097:blood*; D012727:Sex Characteristics
D005979:Glutathione Peroxidase; D012643:Selenium