[Analysis of human DNA fingerprints by using fluorescein labelled probe JH12.6].
A method of analysis of human DNA fingerprints has been established by using fluorescein (Fl-11-dUTP) labelled probe JH12.6. The clear and high distinctive patterns of human DNA fingerprints were obtained with this method. The DNA fingerprints of 78 unrelated individuals living in Yunnan province of China were detected, and their probability of chance association between random individuals was calculated as 7.4 x 10(-11), and their mean allelic frequency as 0.09. Comparing of DNA fingerprints with labelling probe JH12.6 by using between radioactive 32P and fluorescein showed that the method by using fluorescein labelled probe JH12.6 was simple, fast, safe and economical. It can totally replace the method by using radioactive 32P labelled probe JH12.6, and it can be broadly applied in forensic medicine and other fields.
Shen
B
B
Faculty of Forensic Medicine, Kunming Medical College.
Zou
L
L
Chu
J
J
Yang
Y
Y
Zou
P
P
D016172:DNA Fingerprinting / Q000379:methods*; D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans
D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D005456:Fluorescent Dyes; C027078:deoxyuridine triphosphate