NMR analysis of the backbone structure of DNA oligomers containing (5R)-/(5S)-[5'-2H]-2'-deoxynucleosides.
In order to distinguish between NMR signals for pro-R and pro-S protons of the 5'-methylene group, DNA oligomers containing [5'-2H]-2'-deoxynucleosides were synthesized. As the deuterium labeled nucleosides were mixtures of [(5'S)-2H]- and [(5'R)-2H]-isotopomers approximately in a ratio of 2:1, the signals du to pro-R and pro-S protons were assigned undoubt-edly.
Ono
A
A
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Kubo
Y
Y
Makita
T
T
Tate
S
S
Kawashima
E
E
Ishido
Y
Y
Kainosho
M
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003853:Deoxyribonucleosides / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism; D013936:Thymidine / Q000737:chemistry
D003853:Deoxyribonucleosides; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003903:Deuterium; D013936:Thymidine