Plasticity of cholinoreceptors of neurons of the common snail after effects on inositol-1,4,5-triphosphate- and CA(2+)-dependant mobilization of stored CA2+ and the level of phosphatidic acid.
The influences on the depth of extinction of the inward current induced by acetylcholine (the ACh-current) of a number of compounds affecting the mobilization of stored Ca2+ and the intracellular level of Ca(2+)-mobilizing second messengers, namely, inositol-1,4,5-trisphosphate (IP3), inositol hexakisphosphate, TMB-8 (an inhibitor of (IP3)-dependent Ca2+ mobilization), tetracaine (an inhibitor of Ca(2+)-dependant mobilization of Ca2+), as well as phospholipase D, which leads to the formation of phosphatidic acid through the hydrolysis of phosphatidylcholine, were investigated in identified RPa3 and LPa3 neurons of the common snail using the two-electrode voltage clamp technique for the recording of the potential on the membrane. The participation of IP3, of IP3-dependant, and Ca(2+)-dependant mobilized intracellular Ca2+, as well as phosphatidic acid in the regulation of the plasticity of the cholinoreceptors of the neurons was demonstrated.
Pivovarov
A S
AS
M. V. Lomonosov Moscow State University, Russia.
D000109:Acetylcholine / Q000502:physiology; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003214:Conditioning, Classical / Q000187:drug effects; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006372:Helix, Snails; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010712:Phosphatidic Acids / Q000378:metabolism*; D010739:Phospholipase D / Q000494:pharmacology; D010833:Phytic Acid / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects*; D013748:Tetracaine / Q000494:pharmacology
D000779:Anesthetics, Local; D002121:Calcium Channel Blockers; D010712:Phosphatidic Acids; D011950:Receptors, Cholinergic; D013748:Tetracaine; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D010833:Phytic Acid; D015544:Inositol 1,4,5-Trisphosphate; D010739:Phospholipase D; D000109:Acetylcholine; D002118:Calcium