Isolation of prostate-derived single cells and cell clusters from human peripheral blood.
The detection of blood-borne prostate cancer (PCA) cells may help with clinical staging and the further understanding of PCA metastases. We discovered prostate-specific antigen (PSA)-positive stained but not PSA mRNA-expressing blood cells by means of cell sorting and PSA reverse transcription-PCR in patients. Therefore, we developed a cytokeratin immunomagnetic method to isolate PSA-positive epithelial cells from the circulating blood of PCA patients. We obtained blood-borne single cells from 6 of 10 PCA patients and clustered cells from 8 of 10 PCA patients. Patients with benign prostate hyperplasia tested negative for cell clusters. The reported isolation method yielded prostate-derived cells or clusters of them from PCA-diagnosed patients.
Brandt
B
B
Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelms-Universität, Münster, Germany.
Junker
R
R
Griwatz
C
C
Heidl
S
S
Brinkmann
O
O
Semjonow
A
A
Assmann
G
G
Zänker
K S
KS
D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000235:genetics; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000235:genetics; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood* / Q000276:immunology / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis
D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D017493:Leukocyte Common Antigens; D017430:Prostate-Specific Antigen