A rapid procedure for elimination of non-polar lipids hampering the usual polar lipid extraction and TLC separation.
A new and rapid method is proposed for extraction of non-polar lipids from tissues where they are present as abundant components which can interfere with the usual procedures of lipid extraction and TLC separation, and hamper, in particular, sulphatide visualization. A solvent more hydrophobic than chloroform, i.e. n-hexane, was utilized to remove the neutral lipids from samples of female rabbit parotid gland, and the n-hexane phase was used for TLC which showed considerable amounts of cholesterol esters, in addition to triglycerides, diglycerides and monoglycerides. The methanol phase, now devoid of non-polar lipids, was utilized to prepare TLC plates in order to separate and visualize the polar lipid fractions, in particular the sulphatides.
Baldoni
E
E
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
Bolognani
L
L
Vitaioli
L
L
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D004971:Estrus / Q000502:physiology; D005260:Female; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000302:isolation & purification*; D010306:Parotid Gland / Q000737:chemistry; D011817:Rabbits; D013433:Sulfoglycosphingolipids / Q000032:analysis
D008055:Lipids; D013433:Sulfoglycosphingolipids