Intracellular distribution of polyamines in human lymphoblastoid cell line during phorbol ester-induced differentiation.
Polyamines such as putrescine, spermidine and spermine play an important role in nucleic acid metabolism. These aliphatic amines display a key role in cell-induced transformation by carcinogen substances. In particular, one of these, the phorbol myristate acetate, provokes cell differentiation and gives an increase of ornithindecarboxylase activity; enzyme regulating the pathways of polyamines. In this study we analyse the trend of the polyamines at cytoplasmic and nuclear level during phorbol treatment. Our results show a correlation between nuclear and cytoplasmic spermine, 3H-Thymidine, 3H-Leucine incorporation and cell cycle phases. These data remark that the polyamines are differently distributed into the cell during the phorbol myristate acetate-mediated differentiation process and that the spermine is down-regulated for to supply the increased protein biosynthesis.
Stabellini
G
G
Istituto di Istologia ed Embriologia Generale, Università di Ferrara, Italy.
Creati
B
B
Di Primio
R
R
Trubiani
O
O
D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007930:Leucine / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000473:pathology; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
D013096:Spermine; D007930:Leucine; D013755:Tetradecanoylphorbol Acetate; D013095:Spermidine; D011700:Putrescine; D013936:Thymidine