title: Site-directed mutagenesis using overlap extension PCR.
authors.affiliation: Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Aiyar
authors.forename: Y
authors.initials: Y
authors.lastname: Xiang
authors.forename: J
authors.initials: J
authors.lastname: Leis
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes