title: Characterization and sequence of the Escherichia coli panBCD gene cluster.
abstract: A 4589 bp DNA segment containing the Escherichia coli panBCD gene cluster was sequenced, and found to contain 6 complete open reading frames. panB, panC, and panD were identified by subcloning and insertional mutagenesis. The orientation of panD was also confirmed by orientation-specific expression of asparate-1-decarboxylase. panB and panC lie adjacent to one another, but are separated from panD by orf3, which is oriented in the opposite direction. Interruptions in the remaining open reading frames did not affect growth on glucose-minimal medium. No significant similarity to sequences in databases was found for orf1 and orf2. Orf3 contained extensive similarity to reading frames defined by E. coli yjiP, yjiQ, yhgA, and yafD. The function of these amino acid sequences is as yet undefined.
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago 60607, USA.
authors.forename: W K
authors.initials: WK
authors.lastname: Merkel
authors.forename: B P
authors.initials: BP
authors.lastname: Nichols
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005968:Glutamate Decarboxylase / Q000235:genetics; D019877:Hydroxymethyl and Formyl Transferases; D005810:Multigene Family; D016366:Open Reading Frames; D010205:Pantothenic Acid / Q000096:biosynthesis*; D010453:Peptide Synthases / Q000235:genetics; D014166:Transferases / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D010205:Pantothenic Acid; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; C021477:3-methyl-2-oxobutanoate hydroxymethyltransferase; C019104:aspartate-alpha-decarboxylase; D005968:Glutamate Decarboxylase; D010453:Peptide Synthases; C022318:pantothenate synthetase