Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT-PCR). HCV RNA was detected in three HCC lines (JHH-1, JHH-4, and JHH-6) and negative-strand viral RNA was found in JHH-4, indicating that there is a putative replicative intermediate of HCV in JHH-4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV-specific PCR products of these three cell lines were determined. The clone from JHH-1 belonged to genotype 1 (1a or 1b), and the clones from JHH-4 and JHH-6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV.
Tsuboi
S
S
Department of Cell Biology, Okayama University Medical School, Japan.
Nagamori
S
S
Miyazaki
M
M
Mihara
K
K
Fukaya
K
K
Teruya
K
K
Kosaka
T
T
Tsuji
T
T
Namba
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology*; D002648:Child; D004279:DNA, Viral; D005260:Female; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D016662:Virus Integration; D017735:Virus Latency
D004279:DNA, Viral; D012367:RNA, Viral