Acidic and basic fibroblast growth factors prolong the in-vitro survival of murine peritoneal macrophages.
To examine the role of fibroblast growth factors on the proliferation present during angiogenic processes, we analyzed the effects of in vitro cell culture with acidic (FGF-1) and basic (FGF-2) fibroblast growth factors on murine peritoneal exudate cell survival. FGF-1 or FGF-2 in the presence of heparin resulted in a significantly greater cell viability for exudate cells at 24 hours of culture relative to heparin alone or medium controls. Cultures supplemented with FGF-1 or FGF-2 plus heparin showed a slight increase in tritiated[3H] thymidine uptake over heparin or medium alone. No specific change in [3H] uridine incorporation or LDH release was detectable between FGF-1, FGF-2, heparin or medium alone cultures. Our results suggest that FGF-1 and FGF-2 may enhance survival both in vitro and in vivo of a subset of peritoneal exudate cells. This attribute may play a role during inflammatory or tumor-like states.
Hackshaw
K V
KV
Department of Internal Medicine, Ohio State University, Columbus 43210, USA.
Trout
A M
AM
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D015398:Signal Transduction
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D006493:Heparin