In vitro selection analysis of trans-acting HDV ribozyme.
In order to identify the functional structure as well as new active variants of the trans-acting genomic ribozyme of human hepatitis delta virus (HDV), we applied an in vitro selection procedure. After 10 generations, a randomized pool of trans-acting ribozymes accumulated in which the secondary structure of each ribozyme confirmed to the pseudoknot model and important bases in single-stranded regions were all conserved. We were surprised that mutated ribozymes derived from genomic sequence were changed to anti-genomic-like sequences. Further investigations of the most active variant confirmed that each mutated base was the most appropriate nucleotide at every position of HDV ribozyme.
Nishikawa
F
F
National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba Science City, Japan.
Chiba
A
A
Shirai
M
M
Fauzi
H
H
Taira
K
K
Kumar
P K
PK
Nishikawa
S
S
D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D016337:RNA, Catalytic; D012367:RNA, Viral