Use of targeted cationic liposomes in enhanced DNA delivery to cancer cells.
Cationic liposomes are considered to be safe vectors for gene transfer, but they are less efficient at delivering DNA to cells when compared with retroviral vectors. Cationic liposomes complexed with DNA were targeted to specific cells in vitro by means of monoclonal antibodies (mAbs) or ligands associated with the liposomes. Significant increases in expression of a beta-galactosidase reporter gene were observed in vitro in mAb-targeted liposomes, compared with non-targeted liposomes, in both an adherent tumor cell line (human adenocarcinoma) and a suspension cell line (human T-lymphoma). Also, use of asialofetuin as a targeting ligand significantly increased expression of the reporter gene in human hepatoma cells. Our results suggest that site-specific targeting of cationic liposomes is a good strategy for increasing both the selectivity and the efficiency of DNA delivery to cells and with further development may lead to targeted DNA delivery in vivo.
Kao
G Y
GY
Department of Pharmacology, University of Alberta, Edmonton, Canada.
Change
L J
LJ
Allen
T M
TM
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000906:Antibodies; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D002412:Cations; D004247:DNA / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D002412:Cations; D008081:Liposomes; D004247:DNA