Comparison of the survival of fresh or cultured pancreatic islets, pseudoislets and single cells following allotransplantation beneath the kidney capsule in non-immunosuppressed diabetic rats.
The purpose of the present study was to examine the effect of culture pretreatment and islet structure on transplantation survival time. Donors for islet isolation were highly inbred male Lewis rats (RT 1(1)). Production of single cells was performed by using EDTA and Trypsin. Pseudo-islets were produced by culturing single cell suspensions at 37 degrees C for 12-14 days. Recipients were BDE rats (RT 1u), made diabetic by streptozotocin injection. 1000-1200 islets (or corresponding amount of single cells or pseudoislets) were transplanted to the subcapsular renal space. Five groups were transplanted. Group 1 (n = 5) received freshly isolated islets of Langerhans. Group 2 (n = 7) received single cells, produced from freshly isolated islets. In group III (n = 7) pseudoislets were transplanted. The animals of group IV received 37 degrees C cultured islets (12-14 days), while group V received single cells consisting of 12-14 day cultured islets at 37 degrees C. The median survival times were: gr. I 7 d.; gr. II 5 d.; gr. III 120 d.; gr. IV 11 d.; gr. V 9 d.. Group III showed a prolongation of allograft survival that was statistically significant compared to all other groups. 4 from 7 animals showed a long-term acceptance. It can be concluded that neither culturing islets at 37 degrees C nor producing single cells achieves long-term acceptance. Transplanting pseudoislets resulted in a long-term acceptance of allograft, without immunosuppression of the host. Three factors may be responsible for this success: Firstly, a reduced number of class-II-antigen positive cells, secondly, metabolic state of rest, and thirdly, the transplantation site.
Wolf-Jochim
M
M
Medical Clinic III and Policlinic, Justus Liebig University, Giessen, Germany.
Wöhrle
M
M
Federlin
K
K
Bretzel
R G
RG
D000818:Animals; D017690:Cell Transplantation / Q000502:physiology*; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D006085:Graft Survival / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000648:ultrastructure; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000502:physiology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew
D000949:Histocompatibility Antigens Class II