Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals.
The quinolone resistance-determining regions (QRDRs) of the gyrA genes of quinolone-resistant clinical and veterinary salmonella isolates were sequenced. Substitutions analogous to a substitution of a Ser to a Phe at position 83 (Ser83-->Phe) and Asp87-->Gly or Tyr in Escherichia coli were found, as was a single novel mutation outside of the QRDR resulting in Ala119-->Glu. The data suggest that gyrA mutations are associated with quinolone resistance in veterinary and clinical salmonella isolates and that the limits of the QRDR may require revision.
Griggs
D J
DJ
Department of Infection, University of Birmingham, United Kingdom.
Gensberg
K
K
Piddock
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D001483:Base Sequence; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D024841:Fluoroquinolones; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D012475:Salmonella / Q000235:genetics*; D017385:Sequence Homology
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II