Isolation and amino acid sequence of a cystatin-type cysteine proteinase inhibitor from bovine hoof.
A low molecular mass cysteine proteinase inhibitor was found and isolated from bovine hoof. Purification of the inhibitor to homogeneity was performed by carboxymethyl-papain-sepharose affinity and DE-52 ion exchange chromatographies. From 15 g of bovine hoof, 0.25 mg of cysteine proteinase inhibitor was isolated. It had 100 amino acid residues and molecular mass of 11 406 Da, and its N-terminus was blocked. The amino acid sequence of the inhibitor demonstrated high similarity with that of the family 1 group, especially bovine thymus-derived cystatin. Thus, in summary, bovine hoof contains a cystatin-type cysteine proteinase inhibitor, presumably a family 1 cystatin.
Tsushima
H
H
Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.
Higashiyama
K
K
Mine
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015891:Cystatins / Q000737:chemistry / Q000302:isolation & purification*; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D006724:Hoof and Claw / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors