Expression of heat shock proteins in the developing rat retina.
Expression of three heat shock proteins (HSPs), HSP70, HSP90, and immunoglobulin heavy chain binding protein (Bip) was examined in the developing rat retina using Northern blot analysis. The expression of the inducible form of HSP70 remained uniformly low throughout the perinatal period until P5 and increased rapidly at P7. On the other hand, the constitutive form of HSP70, HSP90, and Bip were expressed constitutively in the rat retina throughout the developmental stage except P3-P5, at which a transient decrease of the expression was observed. The increase of inducible HSP70 mRNA at P7 may correspond to the functional maturation of photoreception in the visual nervous system and may be one of the stress responses to photostimulation. The potential roles of each HSP during development of the rat visual system are discussed.
Kojima
M
M
Department of Neurosurgery, Kyoto University, Japan.
Hoshimaru
M
M
Aoki
T
T
Takahashi
J B
JB
Ohtsuka
T
T
Asahi
M
M
Matsuura
N
N
Kikuchi
H
H
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics; D000091342:Endoplasmic Reticulum Chaperone BiP; D019922:GAP-43 Protein; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D016900:Neurofilament Proteins / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000196:embryology / Q000502:physiology*
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D019922:GAP-43 Protein; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins