Distribution of thromboxane A2 receptor mRNA along the mouse nephron segments.
Thromboxane A2 (TXA2) receptor mRNA was semi-quantitated in microdissected mouse nephron segments by reverse transcription-polymerase chain reaction (RT-PCR). TXA2 receptor mRNA was most abundantly expressed in the glomerulus, followed by the distal convoluted tubule > proximal tubule, thick ascending limb of Henle, and outer medullary collecting duct. These results suggest the potential physiological roles of TXA2 in the entire renal tubule as well as in the glomerulus.
Asano
K
K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Taniguchi
S
S
Nakao
A
A
Maruyama
T
T
Watanabe
T
T
Kurokawa
K
K
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D015139:Blotting, Southern; D017931:DNA Primers; D004210:Dissection / Q000379:methods; D007678:Kidney Glomerulus / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D007686:Kidney Tubules, Distal / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008138:Loop of Henle / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009399:Nephrons / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D017482:Receptors, Thromboxane / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
D000199:Actins; D017931:DNA Primers; D012333:RNA, Messenger; D017482:Receptors, Thromboxane