Synergism of Ce (IV) and Pr(III) for the fastest non-enzymatic DNA hydrolysis ever.
By the synergistic catalysis of Ce4+ and Pr3+, DNA is efficiently hydrolyzed at so far the highest rate. When [Ce4+] = 10 mM and [Pr3+] = 5 mM, the half-life of dinucleotide hydrolysis at pH 7 and 50 degrees C is only 24 min. This catalytic activity is 9 times as large as that of Ce4+, which is the only prominent catalyst hitherto reported. The Ce(4+)-Nd3+ combination is also effective. These bimetallic catalytic systems are eminent tools for the future molecular biology and biotechnology.
Takeda
N
N
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Irisawa
M
M
Imai
T
T
Yashiro
M
M
Komiyama
M
M
D002384:Catalysis; D002563:Cerium; D004247:DNA / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry; D004357:Drug Synergism; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D009354:Neodymium; D011221:Praseodymium
D015226:Dinucleoside Phosphates; D007202:Indicators and Reagents; C017758:thymidylyl-(3'-5')-thymidine; D009354:Neodymium; D002563:Cerium; D004247:DNA; D011221:Praseodymium