A mass spectrometric investigation of the reaction between 4,4'-vinylenedipyridine bis[2,2':6',2"-terpyridine platinum(II)] and the self-complementary oligonucleotide d(CpGpTpApCpG).
4,4'-Vinylenedipyridine bis[2,2':6',2"-terpyridine platinum(II)], a potential intermolecular bis-intercalator of DNA, reacts slowly at PtII, the linker being displaced by nucleobases. Crystallization of 4,4'-vinylenedipyridine bis[2,2':6',2"-terpyridine platinum(II)] in the presence of the self-complementary oligonucleotide d(CpGpTpApCpG) gave a product which was analyzed by electrospray ionization mass spectrometry. Molecular mass measurements demonstrated ligation of one terpyridine PtII, which was shown by liquid chromatography-mass spectrometry to reside exclusively on deoxyguanosine. The terpyridine PtII was shown by tandem mass spectrometry to be attached to the 3'-terminal deoxyguanosine, consistent with N-7 substitution.
Lowe
G
G
Dyson Perrins Laboratory, Oxford University, U.K.
McCloskey
J A
JA
Ni
J
J
Vilaivan
T
T
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D003849:Deoxyguanosine; D009841:Oligonucleotides; D009944:Organoplatinum Compounds; D016339:Spectrometry, Mass, Fast Atom Bombardment
C103394:4,4'-vinylenedipyridine bis(2,2'-6,2''-terpyridineplatinum(II)); D009841:Oligonucleotides; D009944:Organoplatinum Compounds; D015082:2,2'-Dipyridyl; D003849:Deoxyguanosine