title: Conditioned taste aversion and protein kinase C in the parabrachial nucleus of rats.
abstract: The enzymatic activity of protein kinase C (PKC) was measured in the cytosol and particulate fraction of parabrachial nucleus, the presumed site of conditioned taste aversion (CTA) engrams. At various time intervals after acquisition of the task (pairing saccharin consumption with subsequent LiCl poisoning) the nucleus was dissected from the frozen coronal sections. An increase (+40%) in the cytosol PKC activity was found 48 h after that pairing in comparison with controls (saline injection instead of LiCl). Particulate enzyme activity virtual did not change (-5%). Thus the total PKC activity increased significantly (21%). Qualitatively similar but less markedly expressed PKC shifts (+18% in cytosol) ere found 24 h following CTA. Twelve hours and 5 days after CTA acquisition the activity and distribution of PKC was similar to that seen in normal rats. The control experiments revealed that 6 h after LiCl injection alone (without previous saccharin consumption) translocation of PKC from the cytosol to the membrane fraction (found previously 1 h after LiCl injection alone) still persisted but did not differ from that found 6 h after its pairing with saccharin drinking (CTA). It is concluded that acquisition of conditioned taste aversion may be followed by synthesis of PKC rather than by its translocation or downregulation.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Krivanek
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000201:enzymology*; D003213:Conditioning, Psychological; D003600:Cytosol / Q000378:metabolism*; D004327:Drinking Behavior / Q000187:drug effects; D018021:Lithium Chloride / Q000494:pharmacology; D008297:Male; D008568:Memory / Q000187:drug effects*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D012439:Saccharin / Q000494:pharmacology; D013649:Taste
chemical_list: D011493:Protein Kinase C; D012439:Saccharin; D018021:Lithium Chloride