Structure and stability of DNA containing alpha-deoxyadenosine.
The alpha-anomer of 2'-deoxyadenosine (alpha) is a major adenine lesion produced by hydroxyl radicals. This lesion was specifically incorporated in duplexes d(TGAGXGTAC).d(GTACNCTCA), where X = alpha and N = A, G, C, or T. The thermodynamic and structural perturbations introduced into DNA by alpha were assessed by UV-melting measurements and molecular mechanics calculations.
Ide
H
H
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Ikeda
H
H
Makino
K
K
Sasaki
M
M
Sugimoto
N
N
D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D003839:Deoxyadenosines / Q000737:chemistry*; D004355:Drug Stability; D017665:Hydroxyl Radical; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D013816:Thermodynamics; D014466:Ultraviolet Rays
D003839:Deoxyadenosines; D009838:Oligodeoxyribonucleotides; D017665:Hydroxyl Radical; D004247:DNA