title: In vitro interaction of coronaviruses with primate and human brain microvascular endothelial cells.
abstract: Primary human and primate brain microvascular endothelial cells were tested for permissiveness to coronaviruses JHM and 229E. While sub-genomic viral RNAs could be detected up to 72 hours post-infection, primate cells were abortively infected and neither virus caused cytopathology. Human cells were non-permissive for JHM but permissive for 229E replication; peak production of progeny 229E and observable cytopathic effects occurred approximately 22 and 32 hour post-infection, respectively. Using the criterion of cytopathology induction in infected endothelial cells, 229E was compared to other human RNA and DNA viruses. In addition, virus induced modulation of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and HLA I was monitored by immunostaining of infected cells.
authors.affiliation: Rocky Mountain Multiple Sclerosis Center, Englewood, Colorado, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Cabirac
authors.forename: R S
authors.initials: RS
authors.lastname: Murray
authors.forename: L B
authors.initials: LB
authors.lastname: McLaughlin
authors.forename: D M
authors.initials: DM
authors.lastname: Skolnick
authors.forename: B
authors.initials: B
authors.lastname: Hogue
authors.forename: K
authors.initials: K
authors.lastname: Dorovini-Zis
authors.forename: P J
authors.initials: PJ
authors.lastname: Didier
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D001921:Brain / Q000098:blood supply*; D002460:Cell Line; D002522:Chlorocebus aethiops; D017934:Coronavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D028941:Coronavirus 229E, Human; D004730:Endothelium, Vascular / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D007700:Kinetics; D008833:Microcirculation; D011323:Primates; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013045:Species Specificity; D013997:Time Factors; D014158:Transcription, Genetic; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012367:RNA, Viral; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1