Sequence analysis of the fiber genomic region of a porcine adenovirus predicts a novel fiber protein.
The complete nucleotide sequence of the putative fiber protein of a porcine adenovirus isolate, NADC-1, was determined. The coding sequence for the fiber protein was found to be 2112 nucleotides, predicting a 703 amino acid protein with a calculated molecular mass of 76,681 Da. The coding sequence is located between 86 and 92.5 map units. A polyadenylation signal was found 44 bases downstream of the stop codon. Northern hybridization analysis identified a band at 2.4 kb, which likely includes the primary transcript plus a tripartite leader (typically found with adenovirus transcripts) and a polyA(+) tail. The predicted NADC-1 fiber protein was found to have a tail domain comparable in size and sequence to most adenovirus fiber proteins, a comparatively short shaft region, and a head region that was more than twice as large as that of other adenovirus fiber proteins. Each of the three structural domains (tail, shaft, and head) of the predicted NADC-1 fiber protein was found to be most similar to the corresponding domain of a different mammalian adenovirus. The NADC-1 fiber head contained an RGD sequence, a motif that is found in the penton protein of other adenoviruses. Furthermore, the predicted amino acid sequence of the C-terminal half of the NADC-1 fiber head has significant homology to S-lectin proteins, a characteristic not shared with other adenovirus fiber proteins. Thus the predicted amino acid sequence of the NADC-1 fiber head is unique among adenoviruses for which the sequence of the fiber protein is known.
Kleiboeker
S B
SB
National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA. skleiboeker@asrr.arsusda.gov
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D002460:Cell Line; D017931:DNA Primers; D017921:Mastadenovirus / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000821:virology; D015678:Viral Structural Proteins
D036022:Capsid Proteins; D017931:DNA Primers; D012367:RNA, Viral; D015678:Viral Structural Proteins; C099240:fiber protein NADC-1, Porcine adenovirus; C018315:hexon capsid protein, Adenovirus