A low molecular weight urokinase derivative with enhanced fibrin affinity.
A Gly-Pro-Arg-Pro tetrapeptide, homologous to amino-terminal segment of the human fibrin alpha chain after the release of the fibrinopeptide A, was covalently coupled to peptide A of low molecular weight urokinase. The resulting derivative gained increased affinity for fibrin. In caseinolytic assay, fibrin can stimulate the derivative to activate plasminogen. The derivative had two-fold greater fibrinolytic potency than native low molecular weight urokinase and its affinity for fibrin clot was 3.9-fold higher than that of low molecular weight urokinase.
Chen
X C
XC
Department of Biochemistry, Nanjing University, China.
Hua
Z C
ZC
Zhu
D X
DX
D002846:Chromatography, Affinity; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D008970:Molecular Weight; D009842:Oligopeptides / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry* / Q000378:metabolism
D009842:Oligopeptides; C034585:glycyl-prolyl-arginyl-proline; D005337:Fibrin; D014568:Urokinase-Type Plasminogen Activator