Quantification of serum proteins using capillary electrophoresis.
Capillary electrophoresis is a technique that can be automated for the separation of charged particles. By investigating suitable sample dilution and injection time and adhering to a strict washing procedure we have been able to quantify paraproteins in serum samples. This has enabled us to use the technique of capillary electrophoresis for the provision of serum protein electrophoresis in a routine clinical laboratory. We present our findings of 260 serum samples, which included 76 samples with paraproteins analysed by both capillary electrophoresis (EC) and high resolution agarose gel electrophoresis (HRAGE). CE was able to detect all the monoclonal bands detected by HRAGE, and in particular, better able to detect IgA monoclonal bands occurring in the beta region. The major advantages of CE over HRAGE relate to the automated nature of CE with the elimination of the need for a densitometer.
Jenkins
M A
MA
Department of Chemical Pathology, Heidelberg Repatriation Hospital, Victoria, Australia.
Guerin
M D
MD
D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D010265:Paraproteinemias / Q000097:blood*; D010266:Paraproteins / Q000032:analysis*
D001798:Blood Proteins; D010266:Paraproteins