title: Determination of phase II drug metabolites in equine urine by micellar electrokinetic capillary chromatography.
abstract: Micellar electrokinetic capillary chromatography (MECC) using diode array detection has been investigated for the determination of phase I and phase II metabolites of drugs in biofluids. Methods were optimised for the determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide, normorphine, meclofenamic acid and its metabolites in equine urine. Solid-phase extraction procedure were developed to concentrate and purify the analytes from spiked and post administration urines for MECC analysis. A simple on-line procedure for monitoring the kinetics of hydrolysis of morphine-glucuronide conjugates by beta-glucuronidase was demonstrated.
authors.affiliation: Horseracing Forensic Laboratory, Newmarket, Suffolk, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: Taylor
authors.forename: S A
authors.initials: SA
authors.lastname: Westwood
authors.forename: D
authors.initials: D
authors.lastname: Perrett
mesh_terms: D000701:Analgesics, Opioid / Q000378:metabolism / Q000652:urine*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D006372:Helix, Snails / Q000201:enzymology; D006736:Horses / Q000378:metabolism / Q000652:urine*; D006868:Hydrolysis; D008469:Meclofenamic Acid / Q000378:metabolism / Q000652:urine*; D008823:Micelles; D009020:Morphine / Q000378:metabolism / Q000652:urine*; D009022:Morphine Derivatives / Q000378:metabolism / Q000652:urine; D009862:Online Systems; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000701:Analgesics, Opioid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D008823:Micelles; D009022:Morphine Derivatives; D008469:Meclofenamic Acid; D009020:Morphine; D005966:Glucuronidase