title: A comparative study of whole-blood platelet aggregation in laboratory animals: its species differences and comparison with turbidimetric method.
abstract: We undertook a systematic comparison of whole-blood platelet aggregation concerning species difference of commonly used laboratory animals (rabbits, guinea pigs, rats and dogs), agonist difference (collagen, ADP and platelet activating factor [PAF] and technical difference against a traditional method of turbidimetry. Collagen-induced aggregation with whole-blood aggregometry was less variable among species tested and was similar to the results obtained by turbidimetry, which measures platelet aggregation in citrated plasma. In contrast, whole-blood aggregometry in responses to ADP and PAF showed marked species difference, being different from turbidimetry. Our results provide species difference among laboratory animals and suggest that the modulations of platelet aggregation by other blood elements probably differ in each species and with stimulating agonists.
authors.affiliation: Toxicology Laboratory, Chugai Pharmaceutical Co., Ltd., Nagano, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kurata
authors.forename: N
authors.initials: N
authors.lastname: Ishizuka
authors.forename: M
authors.initials: M
authors.lastname: Matsuzawa
authors.forename: K
authors.initials: K
authors.lastname: Haruta
authors.forename: K
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D003094:Collagen / Q000494:pharmacology; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D006403:Hematologic Tests; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
chemical_list: D010972:Platelet Activating Factor; D003094:Collagen