title: Cosmids and transcribed sequences from chromosome 11q23.
abstract: To obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome 11 was prepared from microcell hybrid A9 (neo11) cells containing a normal human chromosome 11 tagged with pSV2neo at 11p11.2. Among 15 radiation hybrid clones isolated, RH(11)-9 which contains a q23 fragment in addition to the neo integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 11q23 and 6 cosmids to 11p11.2. Exon amplification proceeded with 23 of the 59 cosmids and 16 putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH(11)-9 cells and putative exons also facilitate the positional cloning of genes in the 11q23 region.
authors.affiliation: Department of Molecular and Cell Genetics, School of Life Sciences, Tottori University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Yawata
authors.forename: S
authors.initials: S
authors.lastname: Kumano
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
authors.forename: A
authors.initials: A
authors.lastname: Kurimasa
authors.forename: H
authors.initials: H
authors.lastname: Kugoh
authors.forename: M
authors.initials: M
authors.lastname: Oshimura
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D003360:Cosmids; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger