title: Characterization of the transmissible gastroenteritis virus (TGEV) transcription initiation sequence. Characterization of TGEV TIS.
abstract: The ability of the TGEV transcription initiation sequence (TIS) to produce subgenomic RNAs was investigated by placing a reporter gene, chloramphenicol acetyltransferase (CAT) under the control of either the mRNA 6 or the mRNA 7 TISs. Both constructs only produced CAT in TGEV infected cells and the amount of CAT produced from the mRNA 7 TIS was less than from the mRNA 6 TIS. Mutations were made within and around the TISs and the effect on CAT production assayed. THe results showed that the TGEV TIS acted as a initiator of transcription for CAT, though the degree of base pairing between the TIS and leader RNA was not the only factor implicated in the control transcription.
authors.affiliation: Division of Molecular Biology, Institute for Animal Health, Newbury, Berkshire, United Kingdom.
authors.forename: J A
authors.initials: JA
authors.lastname: Hiscox
authors.forename: K L
authors.initials: KL
authors.lastname: Mawditt
authors.forename: D
authors.initials: D
authors.lastname: Cavanagh
authors.forename: P
authors.initials: P
authors.lastname: Britton
mesh_terms: D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D017931:DNA Primers; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine; D014158:Transcription, Genetic; D005760:Transmissible gastroenteritis virus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase