title: Targeting nucleic acid secondary structures by antisense oligonucleotides designed through in vitro selection.
abstract: Using an in vitro selection approach, we have isolated oligonucleotides that can bind to a DNA hairpin structure. Complex formation of these oligonucleotides with the target hairpin involves some type of triple-stranded structure with noncanonical interaction, as indicated by bandshift assays and footprinting studies. The selected oligomers can block restriction endonuclease cleavage of the target hairpin in a sequence-specific manner. We demonstrate that in vitro selection can extend the antisense approach to functional targeting of secondary structure motifs. This could provide a basis for interfering with regulatory processes mediated by a variety of nucleic acid structures.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Université Bordeaux II, France.
authors.forename: R K
authors.initials: RK
authors.lastname: Mishra
authors.forename: R
authors.initials: R
authors.lastname: Le Tinévez
authors.forename: J J
authors.initials: JJ
authors.lastname: Toulmé
mesh_terms: D001665:Binding Sites; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000737:chemistry*
chemical_list: D016376:Oligonucleotides, Antisense