title: Cellular localization and regulation of CHIF in kidney and colon.
abstract: Channel inducing factor (CHIF) is a novel cDNA recently cloned from a rat distal colon cDNA library of dexamethasone-treated animals. While its expression in Xenopus oocytes evokes a potassium channel activity similar to that induced by Isk (minK), its cellular role is not clear. CHIF exhibits significant homologies with proteins that are putatively regulatory (phospholemman, gamma-subunit of Na(+)-K(+)-ATPase, Mat-8) while it differs from the small-conductance potassium channel Isk. We have studied the tissue specificity of CHIF expression in rat by in situ hybridization. CHIF is selectively present in the distal parts of the nephron (medullary and papillary collecting ducts and end portions of cortical collecting tubule) and in the epithelial cells of the distal colon. No expression of CHIF was found in renal proximal tubule, loop of Henle and distal tubule, proximal colon, small intestine, lung, choroid plexus, salivary glands, or brain. To gain some insight into CHIF function, we have investigated, using in situ hybridization and ribonuclease protection assay, whether CHIF mRNA expression could be altered in some situations. In the distal colon, corticosteroid hormones, sodium restriction, low-potassium diet, and metabolic acidosis significantly increased CHIF mRNA expression. In the kidney, metabolic acidosis was the only condition that showed an increase in CHIF mRNA expression. Some of these treatments also altered the expression of the colonic H(+)-K(+)-ATPase mRNA. In summary, CHIF mRNA is selectively expressed in the medullary collecting duct of the kidney and in the epithelium of the distal colon; its expression varies differently in these two target tissues after alterations in corticosteroid status, potassium depletion, and metabolic acidosis. The precise cell-specific functions of CHIF remain to be established.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U. 246, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Capurro
authors.forename: N
authors.initials: N
authors.lastname: Coutry
authors.forename: J P
authors.initials: JP
authors.lastname: Bonvalet
authors.forename: B
authors.initials: B
authors.lastname: Escoubet
authors.forename: H
authors.initials: H
authors.lastname: Garty
authors.forename: N
authors.initials: N
authors.lastname: Farman
mesh_terms: D000818:Animals; D003106:Colon / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D015221:Potassium Channels / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C094337:Fxyd4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D015221:Potassium Channels; D012333:RNA, Messenger; D017506:H(+)-K(+)-Exchanging ATPase