title: DNA polymorphisms of lipogenesis genes and analysis of linkage with fatness in turkeys.
abstract: DNA variability of lipogenesis genes was evaluated by Restriction Fragment Length Polymorphism (RFLP) on four selected lines of turkeys. Thirteen polymorphisms were obtained, four on the acetyl-coenzyme A carboxylase gene, one on the apoprotein AI gene, two on the malic enzyme gene, four on the fatty acid synthase gene, and two on the stearoyl-coenzyme A mesaturase gene. Six of these RFLP were studied in more detail on 64 female turkeys sampled in two lines. Large differences in terms of number of DNA profiles and haplotype frequencies were observed between the two genes, in accordance with the variability of recorded fatness performances. An additive model including the effects of RFLP profiles was used to assess linkage between polymorphisms on lipogenesis genes and fatness measured either in vivo by an ultrasonic instrument or, after slaughter, by the ratio of skin plus subcutaneous fat weight to the whole leg weight. In each line, two polymorphisms suggested a statistical association with fat deposit, one on the malic enzyme gene detected by DNA digestion with HindIII and one on the fatty acid synthase gene detected by MspI. A multiple comparison of means associated with each RFLP profile enabled the identification of two lean profiles in one strain, and a fat profile in the other.
authors.affiliation: Laboratoire de Génétique, Institut National de la Recherche Agronomique, Ecole Natinale Supérieure Agronomique de Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Sourdioux
authors.forename: M
authors.initials: M
authors.lastname: Douaire
authors.forename: Y
authors.initials: Y
authors.lastname: Delabrosse
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics; D001345:Autoradiography / Q000662:veterinary; D001823:Body Composition / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D064429:Fatty Acid Synthases / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D006239:Haplotypes; D050356:Lipid Metabolism; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D014422:Turkeys / Q000235:genetics* / Q000254:growth & development
chemical_list: D016632:Apolipoprotein A-I; D004247:DNA; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase