Mechanism of Bradykinin-induced histamine release from rat peritoneal mast cells.
Bradykinin at concentrations higher than 2 microM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a histamine release of the same potency as bradykinin when applied alone. These results indicate that bradykinin-induced histamine release is not attributable to a bradykinin receptor.
Zhao
Q E
QE
Department of Pharmacology, Okayama University, Japan.
Mihara
T
T
Sugimoto
Y
Y
Kamei
C
C
D000818:Animals; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006636:Histamine Release / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D008274:Magnesium; D001920:Bradykinin; D002118:Calcium