title: Interference by gliclazide in the glucose oxidase/peroxidase method for glucose assay.
abstract: Gliclazide interferes with the glucose determination using the glucose oxidase/peroxidase (EC 1.1.3.4/1.11.1.7) (GOD-PERID) method utilizing 2,2-azino-di-(3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) as the oxygen acceptor chromogen. There was an essentially linear relationship between the concentrations of gliclazide and decreasing glucose readings. One mu mol/1 of gliclazide in samples leads to an apparent loss of about 2.5 mu mol/l of glucose. However, gliclazide did not interfere with the glucose determination using the hexokinase/glucose-6-phosphate dehydrogenase method. This interference in the GOD-PERID method for glucose assay can occur in the in vitro experimental samples and cause underestimation of the glucose values. It is suggested that careful attention should be paid to the limited applicability of the GOD-PERID method for glucose assay.
authors.affiliation: The Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Nakashima
authors.forename: J
authors.initials: J
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Hamada
authors.forename: N
authors.initials: N
authors.lastname: Koh
authors.forename: F
authors.initials: F
authors.lastname: Sakakibara
authors.forename: N
authors.initials: N
authors.lastname: Hotta
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D001786:Blood Glucose / Q000032:analysis*; D004347:Drug Interactions; D005907:Gliclazide / Q000737:chemistry*; D005949:Glucose Oxidase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007202:Indicators and Reagents / Q000737:chemistry*; D008297:Male; D009994:Osmolar Concentration; D010544:Peroxidases / Q000378:metabolism; D051381:Rats; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D001786:Blood Glucose; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D005954:Glucosephosphate Dehydrogenase; D005949:Glucose Oxidase; D010544:Peroxidases; C023057:glucose peroxidase; D006593:Hexokinase; D005907:Gliclazide