Effects of tamoxifen and ICI 164384 on protein synthesis and vectorial secretion in polarized rat uterine epithelial cells.
A polarized, primary cell, bicameral culture system was utilized to test the effects of two types of antiestrogens, tamoxifen (class I) and ICI 164384 (class II) on post-mitotic, uterine epithelial cells. The results demonstrate that in addition to blocking estrogen action in a dose-dependent manner, each of these compounds has independent effects on protein synthesis and secretion in these cells. The effects of both tamoxifen and ICI 164384 on vectorial protein secretion were completely opposite to those of estradiol. Both antiestrogens given alone repressed apical secretion and stimulated basal secretion, whereas estradiol stimulated apical and repressed basal secretion. Furthermore, specific protein bands in both the apical and basal secretory compartments responded differently according to dose to each compound. These experiments using polarized uterine epithelial cell cultures have identified apical and basal protein secretion as two cellular response with increased sensitivity to steroids and antisteroids.
Wang
G
G
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Mulholland
J
J
Glasser
S R
SR
D000818:Animals; D016764:Cell Polarity; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D053284:Polyunsaturated Alkamides; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013629:Tamoxifen / Q000494:pharmacology*; D014599:Uterus / Q000378:metabolism*
D004965:Estrogen Antagonists; D053284:Polyunsaturated Alkamides; D011506:Proteins; D013629:Tamoxifen; D004958:Estradiol; C051781:ICI 164384