Analogs of cord red blood cell membrane components displayed on placenta, amnion and amniotic cells in culture.
Placenta and amnion were analyzed to ascertain the presence of antigens in common with red blood cells (RBC) from cord or fetuses. The expression of distinct antigens displayed on a subpopulation of cord RBC and detected by anticord RBC membrane antibodies was particularly investigated, concomitantly with the presence of transferrin receptors (TR) by employing immunohistochemistry. The placenta showed both cord antigen and TR; on the contrary, amnion--which was labelled by anti-cord RBC membrane antibodies--was not stained by the anti-TR antibody. The results of inhibition and double labelling assays further excluded TR as the relevant antigen in the labelling of both placenta and amnion. The staining of fetal membranes disappeared after absorption of antibodies with cord RBC membranes. These results suggest that the antigens externally expressed on a subpopulation of cord RBC are shared by amnion and placenta.
Lunghi
M
M
Istituto di Patologia Generale, Università di Ferrara, Italy.
Giuliani
A L
AL
Melandri
P
P
Ferron
P
P
Cavazzini
L
L
Stabellini
G
G
Vesce
F
F
Berti
G
G
D000650:Amnion / Q000737:chemistry* / Q000166:cytology; D000653:Amniotic Fluid / Q000737:chemistry* / Q000166:cytology; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000737:chemistry* / Q000166:cytology; D011247:Pregnancy
D000954:Antigens, Surface