Gene probe-based detection of type E botulinum neurotoxin binding protein using polymerase chain reaction.
Using primers based on the nucleotide sequence of a neurotoxin binding protein from Type E Clostridium botulinum cultures, an amplified DNA product was obtained through polymerase chain reaction. The 400 base pair amplified DNA fragment was detectable with as low as 0.1 pg template DNA from Type E C. botulinum, and its fidelity was confirmed by Southern blotting using a DNA probe designed to detect the expected amplified DNA fragment. On the other hand, no DNA amplification was observed with as high as 10 ng template DNA from related Types A and B C. botulinum or from C. tetani, indicating the specificity of the probe.
Singh
B R
BR
Department of Chemistry, University of Massachusetts, Dartmouth 02747, USA.
Barcomb-Caddle
L A
LA
Fu
F N
FN
Li
B
B
D001905:Botulinum Toxins / Q000032:analysis / Q000378:metabolism*; D002352:Carrier Proteins; D003014:Clostridium botulinum / Q000378:metabolism; D003470:Culture Media; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D009498:Neurotoxins / Q000032:analysis / Q000378:metabolism*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D002352:Carrier Proteins; D003470:Culture Media; D017931:DNA Primers; D009498:Neurotoxins; D015345:Oligonucleotide Probes; D004247:DNA; D001905:Botulinum Toxins