Aptameric inhibition of in vitro DNA polymerization by phosphorothioate oligonucleotides.
DNA polymerization in the presence of phosphorothioate oligonucleotides (S-oligomers) was investigated. Oligonucleotides consisting of three or four consecutive guanosine residues (G4 and G3, respectively) inhibited chain elongation by AMV reverse transcriptase or Sequenase (polymerase function of T7 DNA polymerase). S-oligomers bearing G4 caused greater inhibition of chain elongation by both enzymes than those with G3. AMV reverse transcriptase was more susceptible to S-oligomers bearing consecutive guanosines than was Sequenase. Electrophoresis on a polyacryl amide gel revealed that G4 and G3-oligomers formed high-order polymeric complexes, and that S-oligomers with G4 formed much more complex than those with G3.
Tamura
N
N
Central Research laboratories, Kaken Pharmaceutical Co. Ltd, Kyoto, Japan.
Iwatani
W
W
Shoji
Y
Y
Shimada
J
J
Mizushima
Y
Y
D001483:Base Sequence; D001704:Biopolymers; D004247:DNA / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013873:Thionucleotides / Q000737:chemistry*
D001704:Biopolymers; D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides; D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase