title: Presence of elevated carcinoembryonic antigen on absorbent disks applied to nipple area of breast carcinoma patients.
abstract: BACKGROUND
  Carcinoembryonic antigen (CEA) is used as a serum marker to detect and monitor the status of various kinds of malignant tumors. To determine whether CEA might be detected in secretions collected topically from around the nipple area, and whether its secretion might differ in a cancerous versus a noncancerous breast, we developed a simple method for collecting and measuring CEA, using a small cellulose membrane disk and an enzyme immunoassay.
  
  
  METHODS
  We measured the amount of CEA excreted from the nipple area of 22 healthy control women and 32 women with unilateral breast carcinoma confirmed histologically. Secretions were collected from the nipple area by affixing a small (20 mm diameter) absorbent disk made of nitrocellulose membrane backed with filter paper to that area for 24 hours. Substances absorbed by the membrane were then subjected to an immunoassay for CEA using anti-CEA antibodies.
  
  
  RESULTS
  In the 22 healthy subjects, a small amount of CEA (0.6 +/- 0.9 units) was secreted from each nipple, which was equally low regardless of the phase of the menstrual cycle. In contrast, 30 of the 32 women with breast carcinoma secreted significantly greater amounts of CEA from the cancerous (16.1 +/- 8.2) than the noncancerous (2.0 +/- 2.2) breast. Such a difference (14.1 +/- 8.0) in CEA excretion was not observed in the healthy controls (0 +/- 0).
  
  
  CONCLUSIONS
  These findings suggest that such disks may provide a simple and noninvasive method of collecting trace molecules, including CEA, in skin secretions around the nipple to evaluate functional disorders of the mammary glands, particularly breast carcinoma. Additional studies are indicated in larger groups of women with various stages of breast carcinoma as well as with benign breast diseases.
authors.affiliation: Department of Dermatology, Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Imayama
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Ueo
authors.forename: S
authors.initials: S
authors.lastname: Nanbara
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Mimori
authors.forename: Y
authors.initials: Y
authors.lastname: Shimozono
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000042:Absorption; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D003101:Collodion; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007124:Immunoenzyme Techniques; D008567:Membranes, Artificial; D008597:Menstrual Cycle; D008875:Middle Aged; D009558:Nipples / Q000378:metabolism* / Q000473:pathology; D010209:Paper
chemical_list: D002272:Carcinoembryonic Antigen; D008567:Membranes, Artificial; D003101:Collodion