title: Theory of gel electrophoresis of DNA: orientational effects.
abstract: Some aspects of the reptation model of gel electrophoresis of DNA, with emphasis on the orientational effects, are considered. A master equation for the reptational dynamics with the end boundary conditions is proposed. Based on this equation, the orientation and dynamics of the persistent-chain are investigated. In particular, the role of trap conformations for this chain, which essentially reduce the chain mobility, was analyzed. A transient orientation of the chain during pulsed field gel electrophoresis is also discussed.
authors.affiliation: Institute of Petrochemical Synthesis, Russian Academy of Science, Moscow, Russia. tips@tips.rc.ac.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Subbotin
authors.forename: A N
authors.initials: AN
authors.lastname: Semenov
mesh_terms: D004247:DNA / Q000032:analysis*; D004586:Electrophoresis; D016521:Electrophoresis, Gel, Pulsed-Field; D008432:Mathematical Computing; D008958:Models, Molecular
chemical_list: D004247:DNA