Hepatic microsomal triglyceride transfer protein messenger RNA concentrations are increased by dietary cholesterol in hamsters.
In hamsters fed high fat diets enriched in trimyristin, tripalmitin or tristearin, increased dietary cholesterol content was associated with increased plasma concentrations of very low density lipoprotein (VLDL) cholesterol and triacylglycerol (p < 0.0001 and p = 0.0017, respectively). Hepatic microsomal triglyceride transfer protein (MTP) mRNA concentration also increased (p < 0.0001), independent of the nature of dietary fat, and was significantly correlated with the plasma VLDL lipid concentrations (p = 0.0002 and p = 0.0106 for cholesterol and triacylglycerol, respectively) and hepatic cholesterol concentrations. Increased expression of the MTP gene may be part of a coordinated response to hepatic cholesterol accumulation leading to increased VLDL lipid secretion.
Bennett
A J
AJ
Department of Biochemistry, University of Nottingham Medical School, UK.
Bruce
J S
JS
Salter
A M
AM
White
D A
DA
Billett
M A
MA
D000704:Analysis of Variance; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D015243:Cholesterol, VLDL / Q000097:blood; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins; D008297:Male; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D014280:Triglycerides / Q000097:blood
D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary; D015243:Cholesterol, VLDL; D006023:Glycoproteins; D012333:RNA, Messenger; D014280:Triglycerides