10Sa RNA is associated with 70S ribosome particles in Escherichia coli.
The intracellular distribution of 10Sa RNA in Escherichia coli was investigated in cell extracts. Northern hybridization revealed that a large fraction of 10Sa RNA cosediments with 70S ribosomes. When 70S ribosomes were dissociated into 50S and 30S subunits in the presence of low levels of Mg2+ ions, almost all of the 10Sa RNA disappeared from both subunits. The extent of the association of the 10Sa RNA with ribosomes was much enhanced during the growth phase of the cells. These results suggest the possibility that 10Sa RNA might function on the ribosomes in E. coli cells.
Komine
Y
Y
Department of Biophysics, Faculty of Science, Kyoto University.
Kitabatake
M
M
Inokuchi
H
H
D001345:Autoradiography; D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle; D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000235:genetics*; D008274:Magnesium; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000528:radiation effects; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism*; D014466:Ultraviolet Rays
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; C096222:tmRNA; D008274:Magnesium