Nafamostat enhances IBMX and secretin-stimulated water and bicarbonate secretion in the isolated, blood-perfused dog pancreas.
1. The effect of nafamostat mesilate, a synthetic protease inhibitor, on pancreatic exocrine secretion was investigated in isolated, blood-perfused dog pancreas. 2. Intra-arterial injections of nafamostat (2-10 mumol) dose-dependently enhanced water and bicarbonate secretion stimulated by 3-isobutyl-1-methylxanthine (IBMX) or secretin, although nafamostat alone did not affect the basal secretion. 3. In the nafamostat-enhanced pancreatic juice, bicarbonate concentrations were increased dose-dependently as compared with the control, while protein concentrations were not significantly changed. 4. These results indicate that nafamostat enhances IBMX and secretin-stimulated water and bicarbonate secretion, which may be mediated through cAMP activation.
Horiuchi
A
A
Second Department of Internal Medicine, Shinshu University, School of Medicine, Matsumoto, Japan.
Iwatsuki
K
K
Chiba
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000818:Animals; D001550:Benzamidines; D001639:Bicarbonates / Q000378:metabolism; D004285:Dogs; D005260:Female; D006146:Guanidines / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000187:drug effects*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012633:Secretin / Q000494:pharmacology*
D001550:Benzamidines; D001639:Bicarbonates; D006146:Guanidines; D011480:Protease Inhibitors; D012633:Secretin; D015056:1-Methyl-3-isobutylxanthine; C032855:nafamostat