Determination of the calcium antagonist SIM6080 and its N- and O-demethylated metabolites in plasma, urine and tissues by high-performance liquid chromatography.
A sensitive and versatile high-performance liquid chromatographic assay for the determination of the calcium antagonist SIM6080 and its four N- and O-demethylated metabolites in plasma, urine and tissues has been developed and validated. A two-step extraction procedure is employed followed by reversed-phase liquid chromatographic analysis using ultraviolet detection. An isomer of SIM6080 was used as the internal standard. The analysis of spiked plasma, urine and tissues demonstrated the accuracy and precision of the assay with quantitation limits of 5 ng/ml (plasma and urine) or 100 ng/g (tissues). This assay has been used for urinary recovery and tissue distribution studies, as well as for toxicokinetic protocols.
Castelnovo
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Medicinal Chemistry Analytical Laboratory, Zambon Group, Milan, Italy.
D002121:Calcium Channel Blockers / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D005029:Ethylenediamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006868:Hydrolysis; D013056:Spectrophotometry, Ultraviolet
D002121:Calcium Channel Blockers; D005029:Ethylenediamines; C063868:SIM 6080