[Tyrosine tRNA(Q*psiA) from bovine liver. Identification of its sites of interaction with homologous aminoacyl-trna synthetase using chemical modification].
Interaction of the bovine liver tyrosine tRNA Q*psi A having short variable loop, with the homologous aminoacyl-tRNA synthetase has been studied by alkylation with ethylnitrosourea. It is shown that tyrosyl-tRNA synthetase (form M.r. 2x39000) protects 3'-phosphates of nucleotides 21 in D-loop, 31 and 44 in anticodon stem and 59-64 T psi C-stem of tRNA Tyr from alkylation. An anticodon of tRNA Tyr did not interact with this proteolytically modified form of tyrosyl-tRNA synthetase (M.r. 2x39000).
Kalachniuk
L H
LH
Korneliuk
O I
OI
Matsuka
H Kh
HKh
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases