Low dose exposure of diethylnitrosamine affects mice liver thymidine kinase.
Swiss albino mice exposed to 5 and 10 mg diethylnitrosamine kg-1 body weight by intravenous route up to four weeks showed cyto- and genotoxic effects. Distortion of cell and nucleus shapes and extensive necrosis were observed. Thymidine kinase activity in the liver declined in diethylnitrosamine dose and duration dependent manners. The adult-form of thymidine kinase isozyme declined continuously during this period. Simultaneously, two isozymic forms of thymidine kinase, with small anodic migrations in an electrophoretic field, were gradually induced. Significance of theses changes in diethylnitrosamine induced precarcinogenic toxicity has been discussed.
Pariat
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Department of Biochemistry, School of Life Sciences, North-Eastern Hill University, Umshing, Shillong, India.
Sharan
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D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007275:Injections, Intravenous; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D013937:Thymidine Kinase / Q000187:drug effects / Q000378:metabolism*
D002273:Carcinogens; D007527:Isoenzymes; D004052:Diethylnitrosamine; D013937:Thymidine Kinase