title: Mode of interaction of loop diuretics with human serum albumin and characterization of binding site.
abstract: OBJECTIVE
  The purpose of this study was to investigate the binding mechanism of loop diuretics with HSA and to characterize the binding site on HSA.
  
  
  METHODS
  Quantitative analysis of potential interaction between ligands bound to HSA was performed by equilibrium dialysis and data for binding of the two ligands to HSA were analyzed on the basis of a theoretical model of simultaneous binding of two ligands.
  
  
  RESULTS
  The binding of loop diuretics is dependent upon the N-B transition, conformational change of albumin. Furthermore, from the results of binding of the drugs to modified HSA, the lysine residue seems to be involved in the binding of loop diuretics to HSA.
  
  
  CONCLUSIONS
  Analysis using models describing independent, competitive, cooperative and anti-cooperative binding led to the conclusion that loop diuretics bind to site I, particularly to the warfarin region on HSA.
authors.affiliation: Department of Pharmacy, Miyazaki Medical College Hospital, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takamura
authors.forename: A
authors.initials: A
authors.lastname: Haruta
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.forename: M
authors.initials: M
authors.lastname: Tsuruoka
authors.forename: K
authors.initials: K
authors.lastname: Yamasaki
authors.forename: A
authors.initials: A
authors.lastname: Suenaga
authors.forename: M
authors.initials: M
authors.lastname: Otagiri
mesh_terms: D001665:Binding Sites; D004232:Diuretics / Q000097:blood* / Q000737:chemistry*; D005456:Fluorescent Dyes; D005665:Furosemide / Q000097:blood / Q000737:chemistry; D006801:Humans; D007052:Ibuprofen / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013449:Sulfonamides / Q000097:blood / Q000737:chemistry; D014859:Warfarin / Q000737:chemistry
chemical_list: D004232:Diuretics; D005456:Fluorescent Dyes; D012709:Serum Albumin; D013449:Sulfonamides; D014859:Warfarin; D005665:Furosemide; C015451:piretanide; D008239:Lysine; D007052:Ibuprofen