Nicotine and cotinine in maternal and neonatal hair as markers of gestational smoking.
OBJECTIVE
To determine the extent of fetal exposure to cigarette smoke, which cannot be clearly extrapolated from maternal reports, the authors measured concentrations of nicotine and cotinine in maternal and neonatal hair and corroborated these measures with maternal history and measures of pregnancy outcome.


METHODS
Prospective study of 94 mother-infant pairs.


METHODS
Newborn nurseries of two hospitals in Toronto.


METHODS
93 mothers, including active smokers, passive smokers and nonsmokers, and their newborns (including one set of twins).


METHODS
Hair collected from mothers and neonates shortly after birth was analysed by radio-immunoassay.


METHODS
Maternal data on demographic variables, obstetric history, diseases, drugs taken and smoking; infant data on demographic variables and birth indicators (gestational age, method of delivery, weight, head circumference, length, presence of meconium, need for resuscitation, need for special care and congenital malformations); hair concentrations of nicotine and cotinine.


RESULTS
Neonates of active smokers had more adverse outcomes than other infants, including lower birth weight, smaller head circumference, shorter length and more perinatal complications. The difference was statistically significant. Amounts of cotinine extracted from mothers' and infants' hair showed significant differences among active smokers, passive smokers and non-smokers. When other people in the household and the mother smoked, neonatal hair concentrations of nicotine and cotinine in the infants were threefold higher than those in the infants of mothers who were the only smokers in their households.


CONCLUSIONS
Hair concentrations of nicotine and cotinine are a powerful biological marker of the extent of intrauterine exposure to tobacco smoke.
Eliopoulos
C
C
Division of Clinical Pharmacology/Toxicology, Hospital for Sick Children, Toronto, Ont.
Klein
J
J
Chitayat
D
D
Greenwald
M
M
Koren
G
G
D000293:Adolescent; D000328:Adult; D003367:Cotinine / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D009538:Nicotine / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011297:Prenatal Exposure Delayed Effects; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*
D009538:Nicotine; D003367:Cotinine