Mitochondrial DNA restriction site map of Cochliomyia macellaria (Diptera:Calliphoridae).
The mitochondrial DNA of the secondary screwworm, Cochliomyia macellaria (F.), was cleaved with 15 restriction endonucleases to produce 54 restriction fragments. Forty-three restriction sites recognized by 12 enzymes were assigned positions on a restriction site map. Both the restriction fragments and the restriction site map were compared with published data from the screwworm, Cochliomyia hominivorax (Coquerel). The restriction site maps were aligned using a combination of highly conserved restriction sites. Estimated nucleotide divergence between the 2 species based on the fragments was 0.052. The divergence based on the restriction site map was 0.093. The data provide a baseline for future population or phylogenetic investigations.
Roehrdanz
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Biosciences Research Laboratory, USDA-ARS, Fargo, ND 58105, USA.
Johnson
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D000818:Animals; D004272:DNA, Mitochondrial / Q000032:analysis*; D004175:Diptera / Q000235:genetics*; D015183:Restriction Mapping
D004272:DNA, Mitochondrial