AgNOR mark epithelial foci in malignant transformation in hamster cheek pouch carcinogenesis.
Hamster cheek pouch mucosa is an accepted model of oral carcinogenesis. We herein examined the value of morphometric evaluation of silver-stained nucleolar organizer regions (AgNOR) in the detection of epithelial foci in malignant transformation following dimethyl-1,2-benzanthracene-induced carcinogenesis of hamster cheek pouch. AgNOR-related parameters were analyzed at different stages of the process of carcinogenesis (control epithelium, epithelium with no unusual microscopic features, "dysplastic" epithelium, exophytic and endophytic carcinomas). Morphometric evaluation of AgNOR revealed incipient cellular alterations which were not evident in routine preparations and contributed to the characterization of different stages of carcinogenesis in this model.
Schwint
A E
AE
Department of Radiobiology, National Atomic Energy Commission, Buenos Aires, Argentina.
Folco
A
A
Morales
A
A
Cabrini
R L
RL
Itoiz
M E
ME
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects; D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D002277:Carcinoma / Q000139:chemically induced / Q000473:pathology; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D002610:Cheek; D004396:Coloring Agents; D006224:Cricetinae; D018450:Disease Progression; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000648:ultrastructure*; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000648:ultrastructure*; D012834:Silver
D002273:Carcinogens; D004396:Coloring Agents; D012834:Silver; D015127:9,10-Dimethyl-1,2-benzanthracene