Influence of cholesterol feeding on bile acid metabolism in young and aged germ-free rats.
The effects of cholesterol feeding on serum and liver cholesterol levels, fecal and biliary bile acid levels, bile acid pool size and bile acid composition were examined in 2-, 12- and 24-month-old male germ-free rats. The major bile acids in these animals were cholic and beta-muricholic acids. Cholesterol feeding increased synthesis of bile acids by 3- to 4-fold, especially that of chenodeoxycholic acid (mainly beta-muricholic acid in the rat), decreasing the cholic acid/chenodeoxycholic acid (CA/CDCA) ratio in all rats regardless of age, even though the CA/CDCA ratio increased as a linear function of age in both diet groups. Cholesterol feeding increased the serum cholesterol level markedly in aged rats. This hypercholesterolemia may be produced by the increase in CA/CDCA ratio in aged rats.
Uchida
K
K
Strategic Information Unit, Shionogi & Co., Ltd., Tokyo, Japan.
Satoh
T
T
Chikai
T
T
Takase
H
H
Nomura
Y
Y
Nakao
H
H
Takeuchi
N
N
D000375:Aging; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism; D005243:Feces / Q000737:chemistry*; D005856:Germ-Free Life / Q000502:physiology*; D007420:Intestine, Large / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats
D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; D002784:Cholesterol; D019826:Cholic Acid