Cytokine production in human and rat macrophages and dicatechol rooperol and esters.
The ability of dicatechol rooperol and esters to inhibit the production of cytokines in endotoxin-stimulated human alveolar macrophages, human blood monocyte/macrophages, histiocytic cell line U937, and rat alveolar macrophages was examined in vitro. Rooperol derivatives inhibited the production of tumour necrosis factor-alpha, interleukin-1 beta and interleukin-6. Of the esters tested on human cells, rooperol diacetate and tetraacetate were more potent inhibitors of cytokine production (IC50 in the range of 10-20 microM) than rooperol disulphate (IC50 in the range of 25-75 microM). The acetate esters also inhibited cytokine production in rat alveolar macrophages, whereas the sulphate had little effect. Rooperol and acetate esters, in the same concentration range, decreased the production of nitric oxide by rat alveolar macrophages stimulated by endotoxin. These concentrations of rooperol had no effect on cell viability, as indicated by incorporation of 14C-labelled leucine into macrophage proteins and their content of lactate dehydrogenase. The results obtained suggest that rooperol esters are potentially useful antiinflammatory agents.
Guzdek
A
A
Institute of Molecular Biology, Collegium Medicum, Jagiellonian University, Cracow, Poland.
Nizankowska
E
E
Allison
A C
AC
Kruger
P B
PB
Koj
A
A
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002396:Catechols / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004952:Esters / Q000494:pharmacology*; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002396:Catechols; D016207:Cytokines; D004952:Esters; D016859:Lipoxygenase Inhibitors; C079659:rooperol