Establishment and characterization of cell lines, MeMS and MeFS, derived from spontaneous rat mesotheliomas.
New cell lines, designated MeMS and MeFS, were established from the epithelial type of mesotheliomas occurring spontaneously in aged male and female Fischer 344 rats, respectively. Morphologically, MeMS and MeFS expressed the epithelial phenotype. Coexpression of keratin and vimentin in both cell lines was confirmed by the immunocytochemistry, one-dimensional gel electrophoresis and immunoblotting for intermediate filament proteins. Hyaluronic acid synthesis of both MeMS and MeFS was demonstrated by the analysis using high-performance liquid chromatography. MeMS and MeFS seem to be useful models for studying the biological nature of rat mesotheliomas.
Shibuya
K
K
Nippon Institute for Biological Science, Tokyo, Japan.
Tajima
M
M
Saitoh
T
T
Shibuya
N
N
Kizaki
H
H
Yamate
J
J
Nunoya
T
T
D000375:Aging; D000818:Animals; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D005260:Female; D008297:Male; D008654:Mesothelioma / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D009368:Neoplasm Transplantation; D015388:Organelles / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D012376:Rodent Diseases; D014185:Transplantation, Isogeneic