N-acetyl-L-cysteine abolishes the bromoethylamine-induced choline incorporation into renal papillary tissue.
The role of regenerative processes in the protective effect of N-acetyl-L-cysteine (NAC) against bromoethylamine-induced renal papillary necrosis was assessed in rats given bromoethylamine (BEA)(1.2 mmol/kg), N-acetylcysteine (6 mmol/kg), or N-acetyl-cysteine plus BEA. Renal papillary slices were dissected after 15 hours of treatment, and 14C-choline incorporation into total phospholipid, lysophosphatidylcholine, sphingomyelin, and phosphatidylcholine was measured. Bromoethylamine elicited an increase in the incorporation of 14C-choline into choline-containing phospholipid, an effect that was abolished when N-acetylcysteine was administered prior to bromoethylamine. These studies indicate that the defensive mechanism of N-acetylcysteine against bromoethylamine-induced renal papillary necrosis is not related to regenerative processes and that N-acetylcysteine abolishes the bromoethylamine-induced choline incorporation into papillary phospholipid.
Thielemann
L E
LE
Departamento de Medicina Experimental, Universidad de Chile, Santiago.
Rodrigo
R A
RA
Oberhauser
E W
EW
Rosenblut
G
G
Videla
L A
LA
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002794:Choline / Q000378:metabolism*; D005021:Ethylamines / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009336:Necrosis; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D013109:Sphingomyelins / Q000378:metabolism
D005021:Ethylamines; D016166:Free Radical Scavengers; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D013109:Sphingomyelins; C004504:2-bromoethylamine; D002794:Choline; D000111:Acetylcysteine