[Selected properties of Bacteroides fragilis enterotoxigenic strains].
Seven B. fragilis strains were examined. One strain was reference, non-enterotoxigenic, representing serotype E2 according to Beerens et al. (1971). Six strains produced enterotoxin (ETBF). Four of them were isolated from human feces and two of them from swine feces. All strains were investigated morphologically, biochemically (ATB Expression, France) and by means of direct immunofluorescence (Bacteroides--IF test, Poland). Their resistance to chemotherapeutics was tested (ATB Expression, France). The MIC values of clindamycin and metronidazole were determined using E test strips (AB Biodisk, Sweden). Each strain formed capsules. The percentage of encapsulated cells was high (80-100%). Some strains produced thick capsules. Biochemical patterns were similar and typical for B. fragilis rods. One enterotoxigenic strain produced gelatinase and three ETBF strains fermented trehalose. All strains reacted in direct immunofluorescence exclusively with conjugate against serotype E of BFG. Thus, each strain showed antigenic pattern E. Drug susceptibility of all strains was similar. One enterotoxigenic strain was resistant to clindamycin. All strains were susceptible to metronidazole. These studies indicate that on the basis of morphological, biochemical and serological features (IF), enterotoxigenic B. fragilis strains cannot be distinguished from the nonenterotoxigenic one. Also, the correlation between toxigenicity and drug sensitivity of the examined strains is not observed.
Rokosz
A
A
Zakład Bakteriologii Klinicznej AM w Warszawie.
Meisel-Mikołajczyk
F
F
D000818:Animals; D001441:Bacteroides fragilis / Q000145:classification* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002981:Clindamycin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004768:Enterotoxins / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D013045:Species Specificity; D013552:Swine
D004768:Enterotoxins; D008795:Metronidazole; D002981:Clindamycin