Interaction of a new depolymerized holothurian glycosaminoglycan with proteins in human plasma.
In a rat template bleeding model, depolymerized holothurian glycosaminoglycan (DHG) prolonged bleeding time at 30 mg/kg i.v. but unfractionated heparin (UFH) had the same effect at 1 mg/kg i.v., indicating that DHG is much less bleeding than UFH. To characterize this difference, we examined the affinity of DHG for plasma proteins by means of a glycosaminoglycan-conjugated cellulofine column in comparison with that of UFH. The DHG column strongly bound factor V, factor IX, protein S, histidine-rich glycoprotein, platelet factor 4 (PF4), beta-thromboglobulin, von Willebrand factor, fibronectin, and heparin cofactor II, but did not bind fibrinogen, prothrombin, factor VII, protein C, antithrombin III (ATIII), plasminogen or alpha 2-plasmin inhibitor. The profile of protein binding to the UFH column was almost the same as that of the DHG column except that ATIII showed affinity for UFH. One of the reasons why DHG caused much less bleeding than UFH is thus suggested to be the differences in their affinity for ATIII in plasma.
Minamiguchi
K
K
Pharmacology Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
Nagase
H
H
Kitazato
K T
KT
Kitazato
K
K
D000818:Animals; D001760:Bleeding Time; D001798:Blood Proteins / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism* / Q000494:pharmacology; D006470:Hemorrhage / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D051381:Rats
D001798:Blood Proteins; D006025:Glycosaminoglycans