In-vitro cell culture models of the nasal epithelium: a comparative histochemical investigation of their suitability for drug transport studies.
OBJECTIVE
To evaluate different in-vitro cell culture models for their suitability to study drug transport through cell monolayers.


METHODS
Bovine turbinate cells (BT; ATCC CRL 1390), human nasal septum tumor cells (RPMI, 2650; ATCC CCL 30), and primary cell cultures of human nasal epithelium were characterized morphologically and histochemically by their lectin binding properties. The development of tight junctions in culture was monitored by actin staining and transepithelial electrical resistance measurements.


RESULTS
The binding pattern of thin-sections of excised human nasal respiratory epithelium was characterized using a pannel of fluorescently-labelled lectins. Mucus in goblet cells was stained by PNA, WGA and SBA, demonstrating the presence of terminal N-acetylglucosamine, N-acetylgalactosamine and galactose residues respectively in the mucus of human nasal cells. Ciliated cells revealed binding sites for N-acetylglucosamine, stained by WGA, whereas Con A, characteristic for mannose moieties, labelled the apical cytoplasm of epithelial cells. Binding sites for DBA were not present in this tissue. Comparing three different cell culture models: BT, RPMI 2650, and human nasal cells in primary culture using three lectins (PNA, WGA, Con A) as well as intracellular actin staining and transepithelial electrical resistance measurements we found, that only human nasal epithelial cells in primary culture showed differentiated epithelial cells, ciliated nasal cells and mucus producing goblet cells, which developed confluent cell monolayers with tight junctions.


CONCLUSIONS
Of the in-vitro cell culture models studied, only human nasal cells in primary culture appears to be suitable for drug transport studies.
Werner
U
U
Department of Pharmaceutics and Biopharmacy, University of Marburg, Germany.
Kissel
T
T
D000199:Actins; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003208:Concanavalin A; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D006801:Humans; D008954:Models, Biological; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D000199:Actins; D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A