Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis.
In the present work we show that a high percentage of the sera from dogs naturally affected with viscero-cutaneous leishmaniasis contain antibodies reacting with the Leishmania infantum P0 ribosomal protein. In order to map the antigenic determinants of the LiP0 protein during Leishmania-infection, the complete amino acid sequence of the protein was synthesized as overlapping 20-mer peptides. We have identified the sequence AAKEEPEESDEDDFGMG, located adjacent to the C-terminal end of the protein, as the major antigenic determinant. The anti-LiP0 antibodies present in the sera of the infected dogs do not cross-react with a relatively similar antigenic determinant of the LiP2 acidic proteins as an indication that the Leishmania P0 protein is an independent immunogenically functional antigen in the canine form of the infection.
Soto
M
M
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Spain.
Requena
J M
JM
Quijada
L
L
Guzman
F
F
Patarroyo
M E
ME
Alonso
C
C
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification; D015153:Blotting, Western; D018076:DNA, Complementary; D004283:Dog Diseases; D004285:Dogs; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D018314:Leishmania infantum / Q000737:chemistry* / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D015800:Protozoan Proteins / Q000276:immunology*; D012269:Ribosomal Proteins / Q000276:immunology*; D016415:Sequence Alignment
D000913:Antibodies, Protozoan; D018076:DNA, Complementary; D000939:Epitopes; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C084989:ribosomal PO protein, Trypanosoma cruzi