title: Extracellular matrix remodelling within the normal human ovarian capsule.
abstract: Collagen tissue content and both interstitial (MMP-1) and type IV collagenases (also known as gelatinases) activity within the normal human ovarian capsule were investigated. The apical tunica albuginea (n = 10) displayed a lower mean total collagen concentration than the ovarian capsule areas (n = 9) with no follicles underneath them (137.8 +/- 36.1 vs. 176.6 +/- 23.1 micrograms/mg wet weight tissue (ww), P = 0.004). This was accompanied by higher net interstitial collagenase activity (12.96 +/- 2.26 vs. 5.97 +/- 1.9 U/g ww, P = 0.016) which was present within the ovarian capsule in active form only. Zymography revealed the dominance of the 72-kDa over the 92-kDa gelatinase form regardless of the capsule area investigated. Our results indicate that extracellular matrix remodelling within human tunica albuginea is more strongly pronounced in the apical region.
authors.affiliation: II Department of Gynaecological Surgery, University School of Medicine, Lublin, Jaczewskiego, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Postawski
authors.forename: T
authors.initials: T
authors.lastname: Rechberger
authors.forename: P
authors.initials: P
authors.lastname: Skorupski
authors.forename: J A
authors.initials: JA
authors.lastname: Jakowicki
mesh_terms: D003094:Collagen / Q000032:analysis; D017364:Collagenases / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D020781:Matrix Metalloproteinase 1; D010053:Ovary / Q000737:chemistry / Q000166:cytology* / Q000502:physiology
chemical_list: D003094:Collagen; D017364:Collagenases; D020781:Matrix Metalloproteinase 1