title: HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC.
abstract: Aqueous alcoholic mallow flower extracts were analyzed both by HPTLC-densitometry in the reflectance mode at 530 nm and by reversed-phase HPLC with gradient elution. For the mallow flower anthocyanins the best chromatographic resolution was obtained by HPLC, which revealed only two main compounds, confirmed by FAB-MS: malvidin 3,5-O-diglucoside (malvin) and malvidin 3-O-(6"-O-malonylglucoside)-5-O-glucoside. The HPTLC densitometric method on cellulose plates provides accuracy, reproducibility and selectivity for the quantitative analysis of the anthocyanins and this method was shown to be much more sensitive than the HPLC-DAD system, at 530 nm. Both methods give comparable quantitative results for total anthocyanins when applied to mallow flowers from two different sources: Italy and Albania.
authors.affiliation: Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Farina
authors.forename: A
authors.initials: A
authors.lastname: Doldo
authors.forename: V
authors.initials: V
authors.lastname: Cotichini
authors.forename: M
authors.initials: M
authors.lastname: Rajevic
authors.forename: M G
authors.initials: MG
authors.lastname: Quaglia
authors.forename: N
authors.initials: N
authors.lastname: Mulinacci
authors.forename: F F
authors.initials: FF
authors.lastname: Vincieri
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D006868:Hydrolysis; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D012015:Reference Standards; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts