Human albumin solders for clinical application during laser tissue welding.
OBJECTIVE
Fifty percent human albumin solder significantly improves weld strength when compared to lower concentrations [Wright et al., ASLMS meeting, April, 1995]. We developed a method for preparing 50% human albumin that may be considered compatible for clinical applications.


METHODS
Fifty percent human albumin solder was prepared from 25% commercially available human albumin using a lyophilization technique. Assessment of sterility, viscosity, pH, and peak absorption wavelength were performed.


RESULTS
This report describes the methodology used to prepare a 50% human albumin solder that is compatible with clinical use. Maintenance of the structural integrity of the albumin was confirmed by polyacrylamide gel electrophoresis.


CONCLUSIONS
This solder preparation can be used alone or with the addition of exogenous chromophores. The final product is sterile, incorporates viral free protocols, maintains high viscosity, and can be applied easily during open or laparoscopic procedures.
Poppas
D P
DP
Children's Hospital, Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA.
Wright
E J
EJ
Guthrie
P D
PD
Shlahet
L T
LT
Retik
A B
AB
D000042:Absorption; D000418:Albumins / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003124:Colorimetry; D004396:Coloring Agents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019793:Fluorescein; D005452:Fluoresceins / Q000737:chemistry / Q000627:therapeutic use; D005456:Fluorescent Dyes / Q000737:chemistry / Q000627:therapeutic use; D005612:Freeze Drying; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007208:Indocyanine Green / Q000737:chemistry / Q000627:therapeutic use; D010535:Laparoscopy; D053685:Laser Therapy; D008751:Methylene Blue / Q000737:chemistry / Q000627:therapeutic use; D013053:Spectrophotometry; D013242:Sterilization; D014014:Tissue Adhesives / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D014783:Viscosity
D000418:Albumins; D004396:Coloring Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D014014:Tissue Adhesives; D007208:Indocyanine Green; D008751:Methylene Blue; D019793:Fluorescein