Quantitative determination and visualization of herpes simplex virus type 1 antigen, free and cell-bound by ELISA.
A method of quantifying and visualizing herpes simplex virus type 1 antigen by indirect enzyme-linked immunosorbent assay (ELISA) is described. This assay is simplified by the use of polyclonal serum and can be applied to the quantification of free antigen as well cell-bound. Moreover, cell viral antigen can be visualized. Antigen sources were viral suspensions, infected cells and proteins extracted from infected cells. The assay was specific and its sensitivity was dependent on the antigen source. The technique was regarded as specific within a range showing a direct correlation (r > 0.8) between the concentration of the antigen and the net absorbance value (the difference of the absorbance obtained with the viral antigen minus the control antigen). The technique has advantages over other ELISA procedures: does not require monoclonal antibodies, or labelled antiviral immunoglobulins or antiviral serum from two different species. In addition total free antigen can be measured.
Tirado
R
R
Universidad Nacional Autónoma de México, Departamento de Microbiología y Parasitología, Facultad de Medicina, México, D.F.
Sarmiento
R E
RE
Gómez
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D002448:Cell Adhesion / Q000276:immunology; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism; D011817:Rabbits; D014407:Tumor Cells, Cultured; D014709:Vero Cells / Q000378:metabolism
D000914:Antibodies, Viral; D000956:Antigens, Viral