title: Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.
abstract: We describe the synthesis of two new substrates for the detection of beta-galactosidase and evaluate their performance in comparison with that of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Of 171 Enterobacteriaceae strains that were able to hydrolyze X-Gal, 166 (97.1%) also hydrolyzed cyclohexenoesculetin-beta-D-galactoside whereas only 96 (56.1%) showed evidence of hydrolysis of 8-hydroxyquinoline-beta-D-galactoside. No false-positive results were observed with either substrate.
authors.affiliation: Department of Chemical and Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom.
authors.forename: A L
authors.initials: AL
authors.lastname: James
authors.forename: J D
authors.initials: JD
authors.lastname: Perry
authors.forename: M
authors.initials: M
authors.lastname: Ford
authors.forename: L
authors.initials: L
authors.lastname: Armstrong
authors.forename: F K
authors.initials: FK
authors.lastname: Gould
mesh_terms: D004755:Enterobacteriaceae / Q000201:enzymology; D005697:Galactosides / Q000138:chemical synthesis / Q000378:metabolism; D006868:Hydrolysis; D006912:Hydroxyquinolines / Q000138:chemical synthesis / Q000378:metabolism; D007202:Indicators and Reagents; D007211:Indoles / Q000378:metabolism; D013379:Substrate Specificity; D014468:Umbelliferones / Q000138:chemical synthesis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis*
chemical_list: C101994:8-hydroxyquinoline-beta-galactoside; D005697:Galactosides; D006912:Hydroxyquinolines; D007202:Indicators and Reagents; D007211:Indoles; D014468:Umbelliferones; C101993:cyclohexenoesculetin-beta-galactoside; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside