Implementation of internal laboratory quality control procedures for the monitoring of ELISA performance at a regional veterinary laboratory.
Quality control (QC) procedures for antigen detection enzyme-linked immunosorbent assays (ELISAs) for hog cholera (HC) virus, foot and mouth disease (FMD) virus, and an antibody detection ELISA for FMD virus were established at a regional veterinary laboratory in northern Thailand. A recently developed computer software package, QCEL, was used to facilitate management and analysis of QC data. The program was used to assess test performance by producing Shewhart-CUSUM control charts which monitored control data for unacceptable fluctuations or trends. QCEL-generated control charts and analyses are presented and discussed. The use of a simple integrated computerised system for storage and analysis of QC control data provided the laboratory with the opportunity to achieve increased confidence in the results of tests performed.
Blacksell
S D
SD
CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia. stuart@aahl.dah.csiro.au
Cameron
A R
AR
Chamnanpood
C
C
Chamnanpood
P
P
Tatong
D
D
Monpolsiri
M
M
Westbury
H A
HA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000032:analysis*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D006691:Classical Swine Fever / Q000276:immunology*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D007753:Laboratories / Q000592:standards*; D011786:Quality Control; D012984:Software; D013552:Swine; D014730:Veterinary Medicine
D000914:Antibodies, Viral; D000956:Antigens, Viral