Effects of four trihalomethanes on DNA strand breaks, renal hyaline droplet formation and serum testosterone in male F-344 rats.
All four possible trihalomethanes (THMs) containing bromine and chlorine, as well as perchloroethylene (PCE), were evaluated for their ability to produce DNA strand breaks, alpha 2u-globulin rich renal deposits, and testosterone changes in male F-344 rats. Rats received daily equimolar doses (0.75 or 1.5 mmol/kg) of THMs or PCE (1000 mg/kg) in 4% Emulphor vehicle by oral gavage for 7 days. No significant DNA strand breaks were produced by any THM or PCE treatment. PCE treatment produced increased hyaline droplet formation in renal tubules. However, all THM treatments reduced or eliminated the appearance of renal hyaline droplets. All four THM treatments also produced a decrease in serum testosterone concentrations on day 7, which might account for decreased hyaline droplet formation. No significant increase in cell proliferation, measured by [3H]thymidine incorporation in vivo, appeared in this 1-week study.
Potter
C L
CL
National Risk Management Research Laboratory, US Environmental Protection Agency, Cincinnati, OH 45268, USA. potter.carl@epamail.epa.gov
Chang
L W
LW
DeAngelo
A B
AB
Daniel
F B
FB
D000818:Animals; D001345:Autoradiography; D001835:Body Weight / Q000187:drug effects; D005617:Chlorofluorocarbons, Methane / Q000633:toxicity*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013739:Testosterone / Q000097:blood*; D000511:alpha-Macroglobulins / Q000096:biosynthesis
D005617:Chlorofluorocarbons, Methane; D000511:alpha-Macroglobulins; D013739:Testosterone; D004247:DNA