Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides.
Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SgII in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is approximately 4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.
Anouar
Y
Y
European Institute for Peptide Research (IFRMP No. 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, France.
Jégou
S
S
Alexandre
D
D
Lihrmann
I
I
Conlon
J M
JM
Vaudry
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D002864:Chromogranins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D010902:Pituitary Gland / Q000737:chemistry; D011498:Protein Precursors / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification; D011895:Rana ridibunda / Q000235:genetics*; D053381:Secretogranin II; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002864:Chromogranins; D018076:DNA, Complementary; D009479:Neuropeptides; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D053381:Secretogranin II; C081159:secretoneurin