Tissue extract from dopamine-depleted striatum enhances differentiation of cultured striatal type-1 astrocytes.
The effects of tissue extract from dopamine (DA)-depleted striatum (lesion extract, L-ext) on morphological and electrophysiological natures of cultured striatal astrocytes were investigated. L-ext treatment suppressed the proliferation of type-1 astrocytes. They became fibrous in a concentration-dependent manner. These changes were not observed in type-2 astrocytes. By whole cell patch-clamp recording, two kinetically and pharmacologically distinct voltage-activated potassium currents, A current and delayed rectifier, were identified. L-ext treatment enhanced both currents in type-1 astrocytes, but only A current in type-2. Data suggest that in tissue extract from DA-depleted striatum, there are increased trophic activities that promote the differentiation of type-1 astrocytes.
Shimano
Y
Y
Department of Physiology, Nagoya City University Medical School, Japan.
Fukuda
A
A
Fujimoto
I
I
Hida
H
H
Nakajima
K
K
Okabe
A
A
Nishino
H
H
D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002457:Cell Extracts; D002478:Cells, Cultured / Q000502:physiology / Q000648:ultrastructure; D004298:Dopamine / Q000172:deficiency*; D007150:Immunohistochemistry; D017072:Neostriatum / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000378:metabolism
D002457:Cell Extracts; D015221:Potassium Channels; D015222:Sodium Channels; D004298:Dopamine