Site-directed mutagenesis using overlap extension PCR.
Aiyar
A
A
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Xiang
Y
Y
Leis
J
J
D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D017931:DNA Primers; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes