title: An asymmetric model for the nucleosome: a binding site for linker histones inside the DNA gyres.
abstract: Histone-DNA contacts within a nucleosome influence the function of trans-acting factors and the molecular machines required to activate the transcription process. The internal architecture of a positioned nucleosome has now been probed with the use of photoactivatable cross-linking reagents to determine the placement of histones along the DNA molecule. A model for the nucleosome is proposed in which the winged-helix domain of the linker histone is asymmetrically located inside the gyres of DNA that also wrap around the core histones. This domain extends the path of the protein superhelix to one side of the core particle.
authors.affiliation: National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2710, USA. awlme@helix.nih.gov
authors.forename: D
authors.initials: D
authors.lastname: Pruss
authors.forename: B
authors.initials: B
authors.lastname: Bartholomew
authors.forename: J
authors.initials: J
authors.lastname: Persinger
authors.forename: J
authors.initials: J
authors.lastname: Hayes
authors.forename: G
authors.initials: G
authors.lastname: Arents
authors.forename: E N
authors.initials: EN
authors.lastname: Moudrianakis
authors.forename: A P
authors.initials: AP
authors.lastname: Wolffe
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012335:RNA, Ribosomal / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D014981:Xenopus
chemical_list: D003432:Cross-Linking Reagents; D006657:Histones; D009707:Nucleosomes; D012335:RNA, Ribosomal; D011994:Recombinant Proteins; D004247:DNA