title: Isolation and pure culture of microvascular endothelial cells from the fetal skin.
abstract: Microvascular endothelial cells were purely isolated from human fetal skin using magnetic particles. The principle of this technique is based on the selective binding of the lectin Ulex europaeus I (UEA I) to the endothelial cell surface via fucose residues. Initially UEA I was covalently bound to tosyl-activated magnetic polydisperse polymer particles (Dynabeads) and then the UEA I-coated beads were collected using a magnetic particle concentrator (MPC). Endothelial cells were isolated by extracting microvascular segments from trypsin-treated fetal skin tissue and were purified by sieving with nylon mesh and by 35% Percoll gradient centrifugation. For further purification, the obtained cells were incubated with UEA I-coated Dynabeads. The endothelial cells bound to the Dynabeads were collected using MPC. This is a simple and reproducible technique for isolating a pure population of microvascular endothelium from the fetal skin.
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: M S
authors.initials: MS
authors.lastname: Cha
authors.forename: D K
authors.initials: DK
authors.lastname: Rah
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005169:Factor VIII / Q000032:analysis; D005260:Female; D005333:Fetus; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D011247:Pregnancy; D012867:Skin / Q000098:blood supply; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D005169:Factor VIII