Inhibition of gamma-aminobutyric acid aminotransferase from rat brain mitochondria by chloride and acetate.
10-100 mmol/l of acetate inhibits gamma-aminobutyric acid aminotransferase from rat brain mitochondria by 35-90%. Similarly, 50-100 mmol/l of chloride inhibits this enzyme by 10-30%. In either case, the inhibition is competitive with GABA and noncompetitive with alpha-ketoglutarate. The effect of sodium and potassium is negligible. gamma-Aminobutyric acid aminotransferase from rat liver mitochondria is also inhibited by acetate and chloride. The degree of inhibition of both hepatic and cerebral enzyme is nearly the same.
Kraus
P
P
Institut für Pharmakologie und Toxikologie, Philipps Universität, Marburg, Germany. krausp@mailer.uni-marburg.de
Noack
G
G
D000612:4-Aminobutyrate Transaminase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000085:Acetates / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology; D002021:Buffers; D002712:Chlorides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051381:Rats
D000085:Acetates; D002021:Buffers; D002712:Chlorides; D004791:Enzyme Inhibitors; D000644:Quaternary Ammonium Compounds; D000612:4-Aminobutyrate Transaminase