Hemoglobin interference with urinary Bence Jones protein analysis on electrophoresis.
Two cases are described which show that on urine protein electrophoresis a paraprotein owing to hemoglobin cannot be distinguished from a Bence Jones protein. A simple method is described for confirming the presence of hemoglobin: a densitometric scan of the electrophoretogram at 415 nm, in which hemoglobin absorbs in a band coincident with the restriction. Furthermore, it is shown that other serum proteins, elevated levels of bilirubin, and Bence Jones protein do not interfere with this detection system.
Hobbs
G A
GA
Department of Pathology, University of Louisville, KY 40206, USA.
Levinson
S S
SS
D001536:Bence Jones Protein / Q000652:urine*; D004586:Electrophoresis; D006456:Hemoglobinuria / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged
D001536:Bence Jones Protein