Induction and activation of procollagenase in rabbit synovial fibroblasts after treatment with active oxygen released by xanthine/xanthine oxidase.
Treatment of rabbit synovial fibroblasts with active oxygen (AO) released by xanthine/xanthine oxidase resulted in an induction of procollagenase in these cells in concentrations ranging from 12.5 micrograms/ml xanthine plus 0.0025 U/ml xanthine oxidase to 50 micrograms/ml xanthine plus 0.01 U/ml xanthine oxidase. Preceding this there was an accumulation of poly(ADP-ribose) for the same concentration range of xanthine/xanthine oxidase. Furthermore, it was found that AO caused activation of the latent procollagenase to the active enzyme in concentrations ranging from 0.1 micrograms/ml xanthine plus 0.00002 U/ml xanthine oxidase to 1 microgram/ml xanthine plus 0.0002 U/ml xanthine oxidase. It is suggested that poly(ADP-ribosyl)ation participates in the induction of procollagenase by relaxing chromatin. Furthermore, it is proposed that AO activates latent procollagenase under physiological conditions.
Ehrlich
W
W
Robert Koch-Institut, Berlin, Germany.
Huser
H
H
Kröger
H
H
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D007375:Interleukin-1 / Q000494:pharmacology; D008954:Models, Biological; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000502:physiology*; D013583:Synovial Membrane / Q000166:cytology*; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism*; D014970:Xanthines / Q000378:metabolism*
D004792:Enzyme Precursors; D007375:Interleukin-1; D017382:Reactive Oxygen Species; D014970:Xanthines; D019820:Xanthine; D011064:Poly Adenosine Diphosphate Ribose; D014969:Xanthine Oxidase; D017364:Collagenases; C022440:procollagenase