Autoradiographic mapping of serotonin 5-HT1A, 5-HT1D, 5-HT2A and 5-HT3 receptors in the aged human spinal cord.
Quantitative autoradiography with selective radioligands was used to establish the respective distribution of serotonin 5-HT1A, 5-HT1D, 5-HT2A and 5-HT3 receptors at the cervical, thoracic and lumbar levels of the spinal cord from subjects who died at 81-94 years. A high density of 5-HT1A receptors, labeled by [3H]8-hydroxy-2-(di-n-propylamino)tetralin ([3H]8-OH-DPAT), was found in the superficial layers of the dorsal horn, with a significant enrichment ( approximately 20%) in the lumbar vs. the thoracic and cervical segments. In contrast, only very low specific labeling by [3H]8-OH-DPAT (i.e. less than 10% of that measured in the dorsal horn), was detected in the ventral horn. 5-HT1D sites labeled by [125I]serotonin-O-carboxymethyl-glycyl-iodo-tyrosinamide ([125I]GTI) were also mainly located within the superficial layers of the dorsal horn, but no difference in their relative density was noted at the three levels of the spinal cord examined. 5-HT2A sites labeled by [3H]ketanserin were found in the dorsal horn of the cervical segments but no specific binding of this radioligand could be detected at any other level of the spinal cord of such aged subjects. Finally, a high density of [3H]S-zacopride-labeled 5-HT3 receptors was noted especially in the most superficial layer (lamina I) of the dorsal horn at all segments examined. These data provide anatomical support for a role of spinal serotonin especially in nociception processing.
Laporte
A M
AM
INSERM U288, Neurobiologie Cellulaire et Fonctionnelle, Paris, France.
Doyen
C
C
Nevo
I T
IT
Chauveau
J
J
Hauw
J J
JJ
Hamon
M
M
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001345:Autoradiography; D006801:Humans; D007650:Ketanserin; D008024:Ligands; D010146:Pain / Q000503:physiopathology; D011180:Postmortem Changes; D011985:Receptors, Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism*
D008024:Ligands; D011985:Receptors, Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D007650:Ketanserin