title: Evidence for the involvement of complement proteins in platelet aggregation by Streptococcus sanguis NCTC 7863.
abstract: We investigated the mechanisms of platelet aggregation by the type strain of Streptococcus sanguis (NCTC 7863). This species is one of the major aetiological agents of infective endocarditis. S. sanguis NCTC 7863 caused aggregation of normal human platelets in vitro following a lag period that varied between donors (7-19 min). Platelet aggregation was dependent on one or more plasma constituents and all the necessary factors gradually became bound to the bacterial surface during the lag period. The length of the lag period was determined by the plasma of the donor and not by a feature of their platelets. Platelet aggregation by S. sanguis NCTC 7863 could be inhibited by heating plasma at 56 degrees C, by treating plasma with cobra venom factor, or by incubating with soluble Complement Receptor 1, all of which inhibit or deplete complement. Complement activation required Mg2+, but not Ca2+ ions and the the cleavage fragment, Ba, of factor B was produced, indicating that the alternative pathway was operative. Zymosan- and S. sanguis-induced aggregation showed similarities, including the same variability in lag times among donors, and absorption of plasma with zymosan prevented the plasma from supporting platelet aggregation by S. sanguis, C3, C9 and vitronectin were found to bind to S. sanguis NCTC 7863, but the latter two were present at very low levels on a non-aggregating strain of S. sanguis, SK96. The rate of assembly of the C5b-9 complex on the NCTC 7863 bacterial surface correlated with the lag time. These data suggest a role for the complement pathway in platelet aggregation by the type strain of S. sanguis.
authors.affiliation: Department of Haematology, Royal Hallamshire Hospital, Sheffield.
authors.forename: I
authors.initials: I
authors.lastname: Ford
authors.forename: C W
authors.initials: CW
authors.lastname: Douglas
authors.forename: J
authors.initials: J
authors.lastname: Heath
authors.forename: C
authors.initials: C
authors.lastname: Rees
authors.forename: F E
authors.initials: FE
authors.lastname: Preston
mesh_terms: D003172:Complement C1 / Q000378:metabolism; D015932:Complement C3c / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D010974:Platelet Aggregation / Q000502:physiology*; D013298:Streptococcus sanguis / Q000502:physiology*; D013997:Time Factors
chemical_list: D003172:Complement C1; D003186:Complement C9; D015938:Complement Membrane Attack Complex; D015932:Complement C3c; D005340:Fibrinogen; D003165:Complement System Proteins