Digestive lipases of the newborn ferret: compensatory role of milk bile salt-dependent lipase.
The amount of mRNA hybridizing to bile salt-dependent lipase and to colipase-dependent lipase probes as well as their translation into active proteins were quantified in the adult and newborn pancreas and lactating mammary gland from the ferret, a species whose milk, similar to that of the human, has bile salt-dependent lipase. The concentration of colipase-dependent lipase mRNA correlated with the amount of activity found in the adult and newborn pancreas, whereas neither mRNA nor activity of this enzyme was detected in the kit pancreas or in the lactating mammary gland. These data indicate that colipase-dependent lipase is actually expressed in adult pancreas and might represent the main lipolytic system in the adult. mRNA hybridizing to the bile salt-dependent lipase probe used in this study were detected in adult and in newborn ferret pancreas as well as in lactating mammary gland. However, the bile salt-dependent lipase activity expressed in the newborn pancreas was very low when compared with the activity expressed either in the mammary gland or in the adult pancreas. These data argue for a compensatory role of milk bile salt-dependent lipase in lipid digestion in the newborn. The hydrolysis of dietary fat might be initiated by preduodenal lipase, the activity of which is only two times lower in the gastric mucosa of the newborn than in the adult ferret. The high concentration of mRNA hybridizing to the bile salt-dependent lipase probe associated with a very poor bile salt-dependent lipase activity and protein suggests either that these mRNA are very unstable or that they are poorly translated into an active pancreatic bile salt-dependent lipase.
Sbarra
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V
INSERM U-260. Unité de Recherche Physiopathologie des Relations Hormono- Nutritionnelles, Faculté de Médecine, Timone, Marseille, France.
Mas
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E
Henderson
T R
TR
Hamosh
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M
Lombardo
D
D
Hamosh
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P
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001647:Bile Acids and Salts / Q000502:physiology*; D015152:Blotting, Northern; D005289:Ferrets; D006801:Humans; D008049:Lipase / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000201:enzymology* / Q000254:growth & development; D008892:Milk / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology* / Q000254:growth & development; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity
D001647:Bile Acids and Salts; D012333:RNA, Messenger; D012313:RNA; D008049:Lipase