title: Quantification analysis of translation initiation signal sequences in vertebrate mRNAs.
abstract: The signal for directing the position of translation initiation in vertebrate mRNAs requires not only ATG initiation codon but also sequences flanking the initiation codon. A consensus sequence, (GCC) GCC (A or G) CCATGG has been proposed for the signal, but actual initiation sequences differ from it in a greater or lesser degree. In the present report, the translation initiation signal sequences of rat preproinsulin mRNA and its mutant mRNAs were analyzed using a quantification method, which demonstrates that strength of the signal plays important role in determining efficiency of initiation reaction.
authors.affiliation: Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Lida
authors.forename: T
authors.initials: T
authors.lastname: Masuda
mesh_terms: D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics*; D007328:Insulin; D016297:Mutagenesis, Site-Directed; D011384:Proinsulin / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
chemical_list: D018387:Codon, Initiator; D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; C013737:preproinsulin; D011384:Proinsulin