title: Evaluation of the concentration and enantiomeric purity of selected free amino acids in fermented malt beverages (beers).
abstract: Even though amino acids are important trace components in the brewing of beers, they have not been extensively evaluated in these beverages. Studies involving the enantiomeric composition of these amino acids are even less prevalent. A brief summary of the brewing process for malt beverages is given. The total concentration and enantiomeric composition of three amino acids (leucine, phenylalanine, and proline) were determined in 25 different beers. Proline tended to have the highest average absolute concentration and the lowest percentage of the D-enantiomer in most samples. In some cases the relative amounts of D-phenylalanine and D-leucine exceeded 10% of the individual amino acids. The enantiomeric composition of the amino acids in different beer samples did not vary as extensively as the absolute concentrations. The reason for the concentration differences between proline and the other amino acids is discussed. A knowledge of amino acid concentrations and enantiomeric compositions appears to be useful in characterizing specific beers and brewing processes.
authors.affiliation: Department of Chemistry, University of Missouri-Rolla 65401, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Ekborg-Ott
authors.forename: D W
authors.initials: DW
authors.lastname: Armstrong
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005449:Fluorenes; D007202:Indicators and Reagents; D007930:Leucine / Q000032:analysis; D010649:Phenylalanine / Q000032:analysis; D011392:Proline / Q000032:analysis; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; D010649:Phenylalanine; D011392:Proline; C054007:1-(9-fluorenyl)methyl chloroformate; D007930:Leucine