title: Differential expression of extracellular matrix components during the morphogenesis of human gastric mucosa.
abstract: BACKGROUND
  Components of extracellular matrix play a crucial role in morphogenesis and epithelial cell differentiation. In this study, we examined the spatiotemporal expression and distribution of several major extracellular macromolecules of developing and adult human gastric mucosa, with particular emphasis on the pit-gland axis.
  
  
  METHODS
  Indirect immunofluorescence was performed on cryosections of developing and adult gastric tissues by using specific antibodies.
  
  
  RESULTS
  From 8 weeks of gestation onward, including adults, heparan sulfate proteoglycan, type IV collagen, and laminin alpha 1, beta 1, and gamma 1 chains were systematically and uniformly located at the basement membrane of the stratified epithelium (8-11 weeks) and of surface and pit-gland epithelia. Between 8 and 20 weeks of gestation, fibronectin and tenascin were colocalized throughout the entire mesenchyme. In adult mucosa, fibronectin was distributed at all levels of surface and glandular epithelium, whereas tenascin expression was restricted to surface and pit epithelial cells. The expression of the alpha 2 laminin chain was first detected at 12 weeks at the base of forming gastric glands. In the adult, the alpha 2 as opposed to the alpha 1 chain of laminin was confined to the basement membrane of the glandular and lower part of gastric pit epithelia.
  
  
  CONCLUSIONS
  This analysis of the composition of the extracellular matrix in human gastric mucosa suggests an important role for some of its components in morphogenesis and maintenance of gastric glands.
authors.affiliation: Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Tremblay
authors.forename: D
authors.initials: D
authors.lastname: Ménard
mesh_terms: D003094:Collagen / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005109:Extracellular Matrix / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005753:Gastric Mucosa / Q000196:embryology*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism
chemical_list: D003094:Collagen; D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007797:Laminin; D011509:Proteoglycans; D019063:Tenascin