title: Isolation of prostate-derived single cells and cell clusters from human peripheral blood.
abstract: The detection of blood-borne prostate cancer (PCA) cells may help with clinical staging and the further understanding of PCA metastases. We discovered prostate-specific antigen (PSA)-positive stained but not PSA mRNA-expressing blood cells by means of cell sorting and PSA reverse transcription-PCR in patients. Therefore, we developed a cytokeratin immunomagnetic method to isolate PSA-positive epithelial cells from the circulating blood of PCA patients. We obtained blood-borne single cells from 6 of 10 PCA patients and clustered cells from 8 of 10 PCA patients. Patients with benign prostate hyperplasia tested negative for cell clusters. The reported isolation method yielded prostate-derived cells or clusters of them from PCA-diagnosed patients.
authors.affiliation: Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Brandt
authors.forename: R
authors.initials: R
authors.lastname: Junker
authors.forename: C
authors.initials: C
authors.lastname: Griwatz
authors.forename: S
authors.initials: S
authors.lastname: Heidl
authors.forename: O
authors.initials: O
authors.lastname: Brinkmann
authors.forename: A
authors.initials: A
authors.lastname: Semjonow
authors.forename: G
authors.initials: G
authors.lastname: Assmann
authors.forename: K S
authors.initials: KS
authors.lastname: Zänker
mesh_terms: D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000235:genetics; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000235:genetics; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood* / Q000276:immunology / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D017493:Leukocyte Common Antigens; D017430:Prostate-Specific Antigen