Role of extended culture time on synergy of fluconazole and human monocyte-derived macrophages in clearing Candida albicans.
Possible enhanced candidacidal synergy of fluconazole and human monocyte-derived macrophages by extended culture time was studied. Synergistic candidacidal activity increased from 1 day to 3 days and was maximal at 6 days. Enhanced synergistic candidacidal activity at 144 h was observed over a 10-fold challenge dose range. Enhanced synergy with culture time was seen with fluconazole-susceptible and -resistant isolates of Candida albicans. These findings emphasize the importance of dosage and an adequate period of therapy.
Gujral
S
S
Department of Medicine, Santa Clara Valley Medical Center 95128, USA.
Brummer
E
E
Stevens
D A
DA
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D013997:Time Factors
D000935:Antifungal Agents; D015725:Fluconazole