In vitro study of a double infection by herpes simplex virus type 2 and Chlamydia trachomatis.
Sexually transmitted diseases (STDs) may recognize multiple etiological agents. Among them, Chlamydia trachomatis (Ct) and herpes simplex virus type 2 (HSV-2) cause symptomatic, subclinical and asymptomatic infections of the urogenital tract which can lead to serious sequelae. In the present study the coinfection and superinfection by Ct and HSV-2 in epithelial cultured cells from human cervix (HeLa 229) are described. A double infection, followed by the intracellular synthesis of chlamydial and viral antigens, was established. Both synergistic and interfering phenomena were recorded: viral antigen synthesis resulted increased whereas Ct inclusion bodies were produced to a lower extent.
Chiarini
F
F
Institute of Microbiology, University of Rome La Sapienza, Italy.
Mansi
A
A
Pisani
S
S
Seganti
L
L
Brunori
S
S
Gentile
V
V
Di Silverio
F
F
D000942:Antigens, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D002692:Chlamydia trachomatis / Q000254:growth & development* / Q000276:immunology; D006367:HeLa Cells / Q000382:microbiology* / Q000821:virology*; D018258:Herpesvirus 2, Human / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D002479:Inclusion Bodies / Q000382:microbiology; D015163:Superinfection / Q000382:microbiology* / Q000821:virology
D000942:Antigens, Bacterial; D000956:Antigens, Viral