Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA.
We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP analysis alone with DNA probe hMFl 87% of 244 donor/recipient pairs revealed different specific bands. By examination of the identical DNA probes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations can be monitored using a combination of RFLP and PCR-AFLP analyses at one locus.
Schreiner
T
T
Red Cross Blood Center, University of Ulm, Germany.
Prochnow-Calzia
H
H
Maccari
B
B
Erne
E
E
Kinzler
I
I
Wölpl
A
A
Wiesneth
M
M
D016026:Bone Marrow Transplantation / Q000276:immunology*; D002678:Chimera / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014184:Transplantation, Homologous
D004247:DNA