title: Ultrastructure of the ankylotic area in the osteopetrotic op/op mouse.
abstract: The op/op mouse, which carries an osteopetrotic mutation, suffers from complete failure of tooth eruption. Commonly the teeth are ankylosed to the bone. Previous reports of ankylosis in the op/op mouse have been based on light microscopy. The aim of this study was to clarify the ultrastructural features of the ankylotic area using the oolong tea extract (OTE) staining technique. The ultrastructural features of ankylosis did not parallel the findings of light microscopy. OTE staining clearly stained the collagen fibers of bone and tooth, and the space dividing the tooth and bone was revealed by transmission electron microscopy. In comparison, light microscopy failed to reveal this space and the ankylosis was unclear. The true ankylotic area was smooth and the tooth was tightly ankylosed to the bone.
authors.affiliation: Department of Pathology, Nihon University School of Dentistry at Matsudo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Sakae
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000844:Ankylosis / Q000473:pathology*; D003094:Collagen / Q000648:ultrastructure; D004396:Coloring Agents; D005419:Flavonoids; D008297:Male; D008334:Mandible / Q000648:ultrastructure; D008336:Mandibular Diseases / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009154:Mutation / Q000235:genetics; D010022:Osteopetrosis / Q000235:genetics / Q000473:pathology*; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D014070:Tooth / Q000648:ultrastructure; D014076:Tooth Diseases / Q000473:pathology*; D014078:Tooth Eruption / Q000235:genetics
chemical_list: D004396:Coloring Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D003094:Collagen