title: Improved procedure for the purification of selenoprotein Ph from human plasma.
abstract: Selenoprotein Ph, the human analogue of selenoprotein P from rat plasma, was purified from human plasma using Heparin Sepharose chromatography, PEG precipitation, DEAE ion exchange chromatography. RP chromatography, SDS-PAGE and electroelution. SDS-PAGE of the purified protein revealed one broad-selenium containing protein band from 56 to 67 kDa with a selenium maximum at 62 kDa. Using a 7.5% T gel this band was separated into two distinct selenium-containing bands with molecular weights of 61 and 64 kDa.
authors.affiliation: Fachrichtung Medizinische Biochemie der Universität des Saarlandes, Homburg/Saar, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Eberle
authors.forename: H J
authors.initials: HJ
authors.lastname: Haas
mesh_terms: D001798:Blood Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D012643:Selenium; D051149:Selenoprotein P; D051140:Selenoproteins
chemical_list: D001798:Blood Proteins; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins; D011092:Polyethylene Glycols; D012643:Selenium