title: Redox state of pyridine nucleotides, but not glutathione, regulate Ca2+ release by H2O2 from mitochondria of pulmonary smooth muscle.
abstract: Treatment of bovine pulmonary artery smooth muscle mitochondria with H2O2 stimulated oxidation of GSH and NAD(P)H along with an increase in Ca2+ release. Addition of oxaloacetate to mitochondrial suspension stimulated Ca2+ release and oxidation of NAD(P)H while GSH level remained unchanged. Subsequently, addition of beta-hydroxybutyrate which reduced mitochondrial pyridine nucleotides caused reuptake of the released Ca2+ without causing appreciable alteration of GSH level. Treatment of the mitochondria with 1,3-bis(2-dichloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase, significantly decreased GSH level without producing discernible change in Ca2+ release and NAD(P)H oxidation.
authors.affiliation: Department of Biochemistry and Biophysics, University of Kalyani, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: Roychoudhury
authors.forename: T
authors.initials: T
authors.lastname: Chakraborti
authors.forename: J J
authors.initials: JJ
authors.lastname: Ghosh
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.forename: S
authors.initials: S
authors.lastname: Chakraborti
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002330:Carmustine / Q000494:pharmacology; D002417:Cattle; D003576:Electron Transport Complex IV / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010071:Oxaloacetates / Q000494:pharmacology; D010084:Oxidation-Reduction; D011651:Pulmonary Artery / Q000378:metabolism*
chemical_list: D010071:Oxaloacetates; D009243:NAD; D009249:NADP; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D005978:Glutathione; D002118:Calcium; D002330:Carmustine