Oxidative modifications of blood serum in humans with coronary artery disease.
Oxidative modifications of blood serum in humans with and without coronary artery disease were investigated. Four parameters were analyzed: the intensity of serum fluorescence, which is indicative of the content of lipofuscine-like lipid peroxidation products; the content of thiobarbituric acid-reactive substances; the lag-phase of serum oxidation by azo-compounds; and the content of lipophilic natural antioxidants--alpha-tocopherol, beta-carotene and ubiquinol-9(10). It was found that coronary artery disease resulted in a significant increase of serum fluorescence and the content of TBARS. The atherogenic disorders in humans with coronary artery disease drastically decreased the lag-phase of serum oxidation in the presence of 2,2'-azo-bis-(2-amidinopropane) dihydrochloride. The oxidative modifications of serum were in close correlation with the balance of natural lipophilic antioxidants in blood serum, i.e. alpha-tocopherol, ubiquinols and beta-carotene. The contents of all antioxidants tested in serum were significantly decreased in patients with coronary artery disease.
Bakalova
R
R
Department of Medical Physics and Biophysics, High University School of Medicine, Sofia, Bulgaria.
Goudev
A
A
Zhelev
Z
Z
Nachev
C
C
D000368:Aged; D000578:Amidines / Q000097:blood; D000704:Analysis of Variance; D015415:Biomarkers; D003327:Coronary Disease / Q000097:blood*; D005260:Female; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood; D014810:Vitamin E / Q000097:blood*; D019207:beta Carotene / Q000097:blood*
D000578:Amidines; D015415:Biomarkers; D005609:Free Radicals; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D014451:Ubiquinone; D014810:Vitamin E; C026663:ubiquinol-10; C046728:2,2'-azobis(2-amidinopropane)