Molecular cloning of a novel mRNA (harderin) specifically and highly expressed in the Harderian gland of the frog, Rana esculenta.
A cDNA clone encoding a novel mRNA (harderin) has been isolated from the cDNA library of frog Rana esculenta Harderian gland. The cDNA is 875 bp long and encodes a novel protein of 218 amino acid residues. The deduced proteins reveals no significant homology to other proteins. The mRNA is a single 0.9 kb transcript detected only in the RNA of frog Harderian gland, highly expressed throughout the year. The constant and exclusive presence of harderin in the frog Harderian gland, suggests a constitutive tissue specific expression for this protein.
Aniello
F
F
Laboratorio di Biochimica e Biologia Molecolare, Stazione Zoologica A. Dohrn, Napoli, Italy.
Branno
M
M
Marra
P
P
Baccari
G C
GC
Di Matteo
L
L
Minucci
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics; D006243:Harderian Gland / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D011893:Rana esculenta; D012621:Seasons; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D011506:Proteins; D012333:RNA, Messenger