Role of amino thiols in luminol chemiluminescence coupled with copper(II)-catalysed oxidation of cysteine and glutathione.
The Cu(II)-catalysed oxidation of the amino thiols cysteine and glutathione with oxygen was examined in the presence of luminol. Light emission was markedly suppressed when the concentration of amino thiols was greater than that of Cu(II). Cysteine caused a delay of chemiluminescence (CL) and no CL emission was observed at high concentrations of GSH. The suppression in CL could be explained in terms of the complexation of the Cu(II) catalyst by formation of a Cu(II)-dicysteine complex in case of cysteine, and of a Cu(II)-GSSG complex in case of GSH.
Kamidate
T
T
Faculty of Engineering, Hokkaido University, Sapporo, Japan.
Kinkou
T
T
Watanabe
H
H
D000588:Amines; D003300:Copper; D003545:Cysteine / Q000737:chemistry*; D005978:Glutathione / Q000031:analogs & derivatives / Q000737:chemistry*; D019803:Glutathione Disulfide; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds
D000588:Amines; D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; D008165:Luminol; D003300:Copper; D005978:Glutathione; D003545:Cysteine; D019803:Glutathione Disulfide