title: Characterization of rpsL and rrs mutations in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities.
abstract: Two genes (rpsL and rrs) with mutations associated with streptomycin resistance in Mycobacterium tuberculosis were characterized in 78 streptomycin-resistant and 61 streptomycin-susceptible isolates recovered from patients living in the United States, South America, Europe, Africa, and Asia. Fifty-four percent of the 78 resistant organisms had missense mutations in codon 43 of rpsL resulting in a K-43-->R substitution. Mutations in codon 88 of rpsL were also identified in four Asian isolates.
authors.affiliation: Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sreevatsan
authors.forename: X
authors.initials: X
authors.lastname: Pan
authors.forename: K E
authors.initials: KE
authors.lastname: Stockbauer
authors.forename: D L
authors.initials: DL
authors.lastname: Williams
authors.forename: B N
authors.initials: BN
authors.lastname: Kreiswirth
authors.forename: J M
authors.initials: JM
authors.lastname: Musser
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017385:Sequence Homology; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000904:Antibiotics, Antitubercular; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin