A spectrophotometric method for the quantitative determination of purity of diethylenetriaminepentaacetic anhydride.
A spectrophotometric method has been developed for the quantitation of purity of diethylenetriaminepentaacetic anhydride (DTPA-A). This spectrophotometric method is conducted by measuring the absorbance at 275 nm of a 1.5 mg/ml solution of DTPA-A in dry dimethylformamide (DMF). The extinction coefficient at 275 nm for DTPA-A in DMF has been determined to be 0.437 ml/cm mg. Quantitation is accomplished by calculation using Beer's law. The assay response is linear for DTPA-A concentrations ranging from 0.05 to 2.0 mg/ml DTPA-A. The assay is specific, precise, and sensitive with a detection limit of 0.015 mg/ml DTPA-A and a quantitation limit of 0.06 mg/ml DTPA-A. The mean recovery from the sample filters employed for sample preparation in this assay is 97.8% (n = 6).
Hekman
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Analytical Research and Development, R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 08869, USA.
D000812:Anhydrides / Q000032:analysis* / Q000302:isolation & purification; D004369:Pentetic Acid / Q000031:analogs & derivatives*; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000812:Anhydrides; D012996:Solutions; D004369:Pentetic Acid