title: Localization of bcl-2, bax, and bcl-x mRNAs in the developing inner ear of the mouse.
abstract: In situ hybridization was employed to study the expression of bcl-2 mRNA and its family members, bax and bcl-x mRNAs, in the developing inner ear. We found that in the cochlear structure, sensory epithelial cells, the spiral ganglion and stria vascularis expressed these mRNAs in postnatal period in a temporally similar manner, but in embryos, neither bax nor bcl-x mRNA were expressed in the sensory epithelium from embryonic day (E) 13 to 19. In contrast to these patterns, bcl-2 mRNA was expressed by E15 to E19, and the expression at E13 was below the lower limit of detection. Non-neuronal tissue (stria vascularis) also expressed these three transcripts during development. These results suggest that bcl-2 family members may be differentially involved in the differentiation of sensory epithelial cells, spiral ganglia, and stria vascularis. In particular, the differential expression patterns in the cochleovestibular neurons suggest that proliferating and differentiating neurons utilize distinct members of the bcl-2 family.
authors.affiliation: Department of Anatomy and Neuroscience, Osaka University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishii
authors.forename: A
authors.initials: A
authors.lastname: Wanaka
authors.forename: K
authors.initials: K
authors.lastname: Ohno
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Eguchi
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D007758:Ear, Inner / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D005314:Embryonic and Fetal Development / Q000502:physiology; D017403:In Situ Hybridization; D051379:Mice; D008813:Mice, Inbred ICR; D009925:Organ of Corti / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D013136:Spiral Ganglion / Q000737:chemistry; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: C494827:Bax protein, mouse; C494812:Bcl2l1 protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein