title: Immune reactivity of cow-asthmatic dairy farmers to the major allergen of cow (BDA20) and to other cow-derived proteins. The use of purified BDA20 increases the performance of diagnostic tests in respiratory cow allergy.
abstract: BACKGROUND
  Cow dust is one of the most important inducers of occupational allergic diseases in Finland. For example, in 1991 it accounted for almost 40% of the new occupational asthma cases.
  
  
  OBJECTIVE
  This study compares the performance of the purified major cow allergen (BDA20) and crude bovine epithelial extract (BEA) in diagnostic tests and examines the role of milk allergy-associated bovine proteins (bovine serum albumin, alpha-lactalbumin, beta-lactoglobulin, casein) in respiratory cow allergy.
  
  
  METHODS
  The humoral responses of cow-asthmatic and healthy farmers to the various components of BEA were analysed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The levels of specific IgE and IgG antibodies were quantificated with enzyme-linked immunosorbent assays (ELISAs). The cellular responses were analysed with antigen-specific lymphocyte proliferation tests.
  
  
  RESULTS
  The specific anti-BDA20 IgE measurement was found to be best in distinguishing between the asthmatic farmers and their healthy colleagues. It proved possible to determine a cut-off value that gave the analysis a specificity and sensitivity of 100%; the distinction between the two groups was highly significant (P < 0.0001). In the lymphocyte proliferation analysis, cow asthma was more closely associated with reactivity to BDA20 than to BEA. In the measurement of anti-BDA20 and anti-BEA IgG antibody levels, considerable overlap between the groups was observed, suggesting that these antibodies are not directly involved in cow allergy. When proteins associated with milk allergy were used as test reagents, no statistically significant differences could be observed between the groups, except for anti-casein IgE antibodies the level of which, however, overlapped considerably between the farmer groups.
  
  
  CONCLUSIONS
  These findings suggest that purified BDA20 is better than BEA for diagnosing cow asthma and that proteins associated with milk allergy are of only marginal significance in this disease.
authors.affiliation: Department of Clinical Microbiology, University of Kuopio, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Virtanen
authors.forename: T
authors.initials: T
authors.lastname: Zeiler
authors.forename: J
authors.initials: J
authors.lastname: Rautiainen
authors.forename: A
authors.initials: A
authors.lastname: Taivainen
authors.forename: J
authors.initials: J
authors.lastname: Pentikäinen
authors.forename: M
authors.initials: M
authors.lastname: Rytkönen
authors.forename: S
authors.initials: S
authors.lastname: Parkkinen
authors.forename: J
authors.initials: J
authors.lastname: Pelkonen
authors.forename: R
authors.initials: R
authors.lastname: Mäntyjärvi
mesh_terms: D000328:Adult; D000382:Agricultural Workers' Diseases / Q000276:immunology*; D000383:Agriculture; D000485:Allergens / Q000276:immunology* / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005387:Finland; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D016269:Milk Hypersensitivity / Q000276:immunology*
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D007073:Immunoglobulin E