title: Quantitative gene expression of two types of glycine transporter in the rat central nervous system.
abstract: Nuclease protection assays were performed to determine the levels of gene expression for the glycine transporters (GLY(T)), GLY(T)-1a, GLY(T)-1b and GLY(T)-2 in select regions of rat CNS. Results showed regional differences in GLY(T)-1a and GLY(T)-1b gene expression throughout the central nervous system (CNS) whereas GLY(T)-2 was predominantly expressed in the caudal brain. Although the distribution of GLY(T) correlates with the regional distribution of the glycine and N-methyl-D-aspartate receptor, areas shown to be highly susceptible to acute ischemia consistently showed low levels of GLY(T) mRNAs.
authors.affiliation: Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Goebel
mesh_terms: D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D017931:DNA Primers; D015870:Gene Expression; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins; D016376:Oligonucleotides, Antisense; D009928:Organ Specificity; D051381:Rats; D012160:Retina / Q000378:metabolism
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D017931:DNA Primers; D050487:Glycine Plasma Membrane Transport Proteins; D016376:Oligonucleotides, Antisense; D005998:Glycine