Transient compartmental expression of a family of protein tyrosine phosphatases in the developing striatum.
The expression of a family of intracellular protein tyrosine phosphatases (STEP) was studied in the striatum of rats during ontogeny. Links between the formation of dopamine islands and STEP immunoreactive patches in the striatum were examined since previous work had suggested that STEP isoforms were selectively expressed in dopaminoceptive brain regions. STEP protein and mRNAs were distributed in a patchy manner during the first postnatal week. By 2 weeks, STEP immunoreactivity was homogeneous, indicating that both patch and matrix neurons express STEP by maturity. Two-color immunofluorescent staining was also performed to compare STEP with specific markers for patch and matrix. Tyrosine hydroxylase immunoreactive fibers from the substantia nigra form distinctive dopamine islands in the striatum during late embryonic development, and occupy the sites of future patches [23,37,38,54]. These fiber islands align with STEP immunoreactive neuronal patches during the first two postnatal weeks, suggesting that STEP is a marker for patch neurons in early postnatal development. When STEP's distribution was compared with other markers for patch (substance P) or matrix (calbindin), STEP co-localized with substance P in most striatal neurons on postnatal days 1 through 7. However, STEP was also expressed within a subset of calbindin-positive neurons in the lateral striatum, but not with these neurons elsewhere in the striatum. By adulthood, STEP colocalized with both markers. These results suggest that STEP is expressed first within patch neurons but not matrix, and subsequently within both. The expression of STEP may be triggered by the arrival of striatal afferents or other regulatory factors.
Raghunathan
A
A
Department of Biology, Wesleyan University, Middletown, CT 06459, USA.
Matthews
G A
GA
Lombroso
P J
PJ
Naegele
J R
JR
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D064026:Calbindins; D002452:Cell Count; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics; D017072:Neostriatum / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D010750:Phosphoproteins; D049590:Postpartum Period / Q000502:physiology; D011247:Pregnancy; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000235:genetics; D013373:Substance P / Q000235:genetics; D013378:Substantia Nigra / Q000502:physiology; D013997:Time Factors; D014158:Transcription, Genetic / Q000502:physiology
D064026:Calbindins; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D064030:S100 Calcium Binding Protein G; D013373:Substance P; D017027:Protein Tyrosine Phosphatases