Respiratory burst is decreased by human hyperlipemic serum in rat peritoneal macrophages.
The effect of hyperlipemic human serum on superoxide anion (O2-) production by rat peritoneal macrophages was investigated. Phorbol myristate acetate (PMA)-stimulated O2- production was inhibited when cells were preincubated with hyperlipemic human serum. This inhibition was specifically carried out by a lipid fraction and was dependent on both cholesterol and triglyceride serum levels. This inhibitory effect was not exerted by a direct effect on NADPH-oxidase activity, nor by a putative superoxide dismutase activity present in the serum. With human neutrophils, we observed a decreased mobility of the cytosolic factor p47-phox to the membrane during the activation process, caused by hyperlipemic serum. We did not find any effect of hyperlipemic serum on NO2- production by cultured rat macrophages. These results suggest that a pathological increase of circulating plasma lipids may be associated with an impaired inflamatory capacity of macrophages.
Conde
M
M
Dpto. Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Spain.
Chiara
M D
MD
Gracia Marquez
M
M
Andrade
J
J
Santa Maria
C
C
Bedoya
F
F
Sobrino
F
F
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D009249:NADP / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood / Q000494:pharmacology
D008055:Lipids; D014280:Triglycerides; D013481:Superoxides; D009569:Nitric Oxide; D009249:NADP; D002784:Cholesterol; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen