Further metabolism of 2-formylaminofluorene, a metabolite of 2-aminofluorene, in rats.
The further in vivo metabolism of 2-formylaminofluorene (FAF), which is a metabolite of 2-aminofluorene, was examined in rats. The 48 h urine collected following oral administration of FAF to rats was treated with beta-glucuronidase/arylsulfatase and extracted with benzene. When the benzene extract was subjected to HPLC, ten peaks due to FAF and its metabolites were detected. Among these metabolites, major metabolites 1, 2, 4 and 5 were isolated and identified unequivocally as 7-hydroxy-2-formylaminofluorene, 7-hydroxy-2-acetylaminofluorene, 5-hydroxy-2-formylaminofluorene and 5-hydroxy-2-acetylaminofluorene, respectively, by comparing their MS and UV spectra, and HPLC and TLC behavior with that of authentic specimens. Minor metabolites, 7-hydroxy-2-aminofluorene, 5-hydroxy-2-aminofluorene, 2-acetylaminofluorene and 2-aminofluorene together with the parent compound were detected by HPLC. These metabolites of FAF were also detected in the feces of rats given FAF.
Ueda
O
O
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Kitamura
S
S
Tatsumi
K
K
D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D005449:Fluorenes; C012177:2-aminofluorene