title: Urinary excretion of thiobarbituric acid reactive substances of healthy subjects supplemented with a high dose of d-alpha-tocopherol.
abstract: The level of urinary thiobarbituric acid reactive substances (TBARS) consisting of bound forms of malonaldehyde and, to a lesser extent, other aldehydes is one of the indices of lipid peroxidation status. Levels of urinary TBARS in healthy subjects before and after supplementation with a high dose of d-alpha-tocopherol were measured using high performance liquid chromatography. Four healthy Japanese were given a supplement of 300 mg d-alpha-tocopherol/d, about 40-fold higher than the normal intake recommended, for a period of 50 d. Levels of urinary TBARS (nmol/kg body weight.h) within-day, before and after supplementation with d-alpha-tocopherol, exhibited similar behavior and levels of daily urinary TBARS (nmol/kg body weight.d) were unchanged by d-alpha-tocopherol supplementation. These results indicate that supplementation with a high dose of d-alpha-tocopherol does not affect the level of urinary TBARS.
authors.affiliation: Ferris University, Yokohama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kosugi
authors.forename: Y
authors.initials: Y
authors.lastname: Asano
authors.forename: T
authors.initials: T
authors.lastname: Nagayama
authors.forename: M
authors.initials: M
authors.lastname: Beppu
authors.forename: K
authors.initials: K
authors.lastname: Kikugawa
mesh_terms: D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D013858:Thiobarbiturates / Q000652:urine*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D013858:Thiobarbiturates; D014810:Vitamin E; C029684:thiobarbituric acid