A quantitative test (HCMV-hybrid-capture(TM)) to detect human cytomegalovirus DNA in the blood of immunocompromised patients compared with antigenemia and polymerase chain reaction.
A quantitative assay is necessary to differentiate between low and high Cytomegalovirus load in the blood, a high load frequently being correlated with clinical disease. A new method for the quantitative determination of viral DNA (HCMV-hybrid-capture) was compared with antigenemia and PCR. Hybrid-capture proved to be a simple and rapid method for the quantitative determination of viral load in the blood. It is less sensitive than PCR and antigenemia, but seems to correlate with clinical symptoms as well as the antigenemia test and better than non quantitative PCR.
Lazzarotto
T
T
Institute of Microbiology, University of Bologna, Italy.
Campisi
T
T
Dal Monte
P
P
Galli
S
S
Spezzacatena
P
P
Guglielmi
P
P
Landini
M P
MP
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D016867:Immunocompromised Host; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D019562:Viral Load
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic