title: Distribution of guanylate cyclase within photoreceptor outer segments.
abstract: Guanylate cyclases play an essential role in the recovery of vertebrate photoreceptor cells after light activation. Here, we have investigated how one such guanylate cyclase, RetGC-1, is distributed within light- and dark-adapted rod photoreceptor cells. Guanylate cyclase activity partitioned with the photoreceptor outer segment (OS) cytoskeleton in a light-sensitive manner. RetGC-1 was found to bind actin filaments in actin blot overlays, suggesting a mechanism for its association with the OS cytoskeleton. In retinal sections, this enzyme was immunodetected only in the OSs, where it appeared to be distributed throughout the disk membranes.
authors.affiliation: School of Optometry, Indiana University, Bloomington 47405, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Hallett
authors.forename: J L
authors.initials: JL
authors.lastname: Delaat
authors.forename: K
authors.initials: K
authors.lastname: Arikawa
authors.forename: C L
authors.initials: CL
authors.lastname: Schlamp
authors.forename: F
authors.initials: F
authors.lastname: Kong
authors.forename: D S
authors.initials: DS
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000201:enzymology; D006162:Guanylate Cyclase / Q000032:analysis* / Q000235:genetics; D015151:Immunoblotting; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D012160:Retina / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D006162:Guanylate Cyclase