Pharmacology of Ca2+ release from red beet microsomes suggests the presence of ryanodine receptor homologs in higher plants.
Cyclic ADP-ribose (cADPR) is known to release Ca2+ from plant vacuoles, implying that this NAD+ metabolite may possess a second messenger role in plants. The degree to which the plant cADPR-gated Ca2+ release mechanism resembles cADPR action in animals has been evaluated. cADPR-elicited Ca2+ release from red beet microsomes was inhibited by 1 mM procaine but insensitive to heparin. Furthermore, pre-release of Ca2+ from red beet vesicles by either 5 mM caffeine or micromolar levels of ryanodine precluded further Ca2+ mobilisation by cADPR. Thus, this study argues strongly for conservation between the plant and animal cADPR-elicited Ca2+ release mechanisms.
Muir
S R
SR
The Plant Laboratory, University of York, UK. srm3@unix.york.ac.uk
Sanders
D
D
D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000737:chemistry; D036563:Cyclic ADP-Ribose; D006493:Heparin / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D008861:Microsomes / Q000378:metabolism*; D009124:Muscle Proteins / Q000737:chemistry; D011343:Procaine / Q000494:pharmacology; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel; D014675:Vegetables / Q000502:physiology*
D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009124:Muscle Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D036563:Cyclic ADP-Ribose; D012433:Ryanodine; D000246:Adenosine Diphosphate Ribose; D002110:Caffeine; D011343:Procaine; D015544:Inositol 1,4,5-Trisphosphate; D006493:Heparin; D002118:Calcium