Effects of glucose on the activity of erythrocyte membrane Ca2+ATPase in subjects with normal and impaired glucose tolerance.
The effects of glucose on Ca2+ATPase activity of erythrocyte membranes were investigated under in vitro and in vivo conditions. Results of the study, in vitro, showed that Ca2+ATPase activity of erythrocyte membrane was decreased by increasing glucose concentrations (0-30 mM). When glucose-6-phosphate (0-100 mM) was used instead of glucose no significant change occurred in Ca2+ATPase activity. Tunicamycin (0.01 ng/ml) when added to the incubation media together with glucose prevented the decrease in Ca2+ATPase activity. The results of the studies in vivo showed that Ca2+ATPase activities of both male and female subjects with normal or impaired glucose tolerance were changed with time in a complex manner after an oral glucose loading. However, Ca2+ATPase activities of the subjects with impaired glucose tolerance were smaller than those of the corresponding normal controls even before glucose administration.
Bilgin
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R
University of Cukurova, Arts & Science Faculty, Department of Chemistry (Biochemistry Division), Balcah-Adana-TURKEY.
Tükel
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SS
D000328:Adult; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005951:Glucose Tolerance Test; D019298:Glucose-6-Phosphate / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014415:Tunicamycin / Q000494:pharmacology
D014415:Tunicamycin; D019298:Glucose-6-Phosphate; D000252:Calcium-Transporting ATPases; D005947:Glucose