Combined histochemical and immunohistochemical determination of three muscle fibre types in a single section of porcine skeletal muscle.
The method of successive staining for SDH and immunohistochemical detection of fast contracting muscle fibres in pig muscle tissue has been used for demonstration of three muscle fibre types (slow oxidative, SO; fast metabolic intermediate, FI; fast glycolytic, FG). It gives basic information on contractile and metabolic muscle fibre properties in a single frozen section and can be well applied to the pale, soft and exudative (PSE) changes affecting pig muscles.
Fazarinc
G
G
Veterinary Faculty, University of Ljubljana, Slovenia.
Majdic
G
G
Lorger
J
J
Pogacnik
A
A
Bavdek
S V
SV
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D005629:Frozen Sections; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D009218:Myosins / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis; D013552:Swine
D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases; D009218:Myosins