Phorbol ester or cAMP enhance thyrotropin-releasing hormone mRNA in primary cultures of hypothalamic cells.
Thyrotropin releasing hormone (TRH) biosynthesis is subject to a multifactorial control. TRH mRNA levels are negatively regulated by thyroid hormones in the paraventricular hypothalamic nucleus, and positively in cold exposure or suckling. Effect of second messenger pathways stimulation, a known response to membrane receptors, was studied in vitro; cultures of rat embryonic hypothalami (18 day gestation) were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA, 100 nM) or dibutiryl cAMP (dBcAMP, 1 mM) for various times. Levels of TRH mRNA were raised after the first hour of dBcAMP or 2 h of TPA treatment and were still increased at 24 h. These results suggest a neural regulation of TRH biosynthesis.
Uribe
R M
RM
Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México.
Pérez-Martínez
L
L
de Lourdes Covarrubias
M
M
Gómez
O
O
Covarrubias
L
L
Charli
J L
JL
Joseph-Bravo
P
P
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013973:Thyrotropin-Releasing Hormone / Q000187:drug effects / Q000235:genetics*
D012333:RNA, Messenger; D013973:Thyrotropin-Releasing Hormone; D003994:Bucladesine; D013755:Tetradecanoylphorbol Acetate