title: Mouse immature oocytes irradiated in vivo at 14-days of age and evaluated for transmitted effects using the aggregation embryo chimera assay.
abstract: A previous study using the mouse-preimplantation-embryo-chimera assay demonstrated a reproducible transmitted effect (proliferation disadvantage observed in early embryos) from females irradiated as 49-day-old adults using 0.15 Gy of gamma rays and then mated seven weeks later, i.e., embryos were from oocytes that were immature at time of irradiation. Because mouse immature oocytes are known to be much more radiosensitive to cell killing in juveniles than in adults, a follow-on study was performed here using 14-day-old juvenile mice. In contrast to adults, the exposure of juveniles to 0.15 Gy of gamma rays did not result in a detectable transmitted proliferation disadvantage when animals were mated 7 or 12 weeks later. This observation is discussed in light of previous studies on mouse immature oocytes and embryo chimeras.
authors.affiliation: University of California Lawrence Livermore National Laboratory, Livermore 94551, USA. straume2@llnl.gov
authors.forename: T
authors.initials: T
authors.lastname: Straume
authors.forename: O G
authors.initials: OG
authors.lastname: Raabe
authors.forename: K J
authors.initials: KJ
authors.lastname: Walsh
authors.forename: L M
authors.initials: LM
authors.lastname: Wiley
mesh_terms: D000367:Age Factors; D000818:Animals; D002678:Chimera / Q000528:radiation effects; D005314:Embryonic and Fetal Development; D005260:Female; D051379:Mice; D009865:Oocytes / Q000528:radiation effects*