Generative cells of Lilium longiflorum possess translatable mRNA and functional protein synthesis machinery.
Metabolic labelling with [35S]-methionine demonstrated that generative cells of Lilium longiflorum possess their own set of mRNA and are capable of synthesising proteins independently from the vegetative cell. The isolated generative cells synthesised ten proteins, of which six were unique to these specialised cells. Isolation of generative cells from pollen grains after [35S]-methionine labelling resulted in an identical protein profile, therefore the synthesis of these proteins was not due to isolation shock. Addition of cycloheximide, abolished TCA-precipitable counts, whilst actinomycin D had no qualitative effect on the observed protein profile, indicating active translation of pre-existing mRNAs by the generative cells.
Blomstedt
C K
CK
School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Knox
R B
RB
Singh
M B
MB
D002469:Cell Separation; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D063245:Plant Development; D010940:Plant Proteins / Q000096:biosynthesis; D011058:Pollen / Q000378:metabolism; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism*; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*
D019384:Nucleic Acid Synthesis Inhibitors; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D018749:RNA, Plant; D003609:Dactinomycin; D003513:Cycloheximide