title: Site-directed mutagenesis using positive antibiotic selection.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Bohnsack
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004260:DNA Repair; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006378:Helper Viruses / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D012641:Selection, Genetic; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded