Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.
Streptomyces ambofaciens DSM40697 possess six rRNA gene clusters which can be distinguished by probing BfrI-digested total DNA with the PCR-amplified l6S-23S ribosomal genet internal transcribed spacer. The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA. For five of the six ribosomal gene sets, the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus.
Berger
F
F
Laboratoire de Génétique et Microbiologie, Unité associée INRA 952, Faculté des Sciences, Université Henri Poincaré-Nancy 1, Vandouvre-lès-Nancy, France.
Fischer
G
G
Kyriacou
A
A
Decaris
B
B
Leblond
P
P
D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family; D009876:Operon; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S