Characterization of rpsL and rrs mutations in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities.
Two genes (rpsL and rrs) with mutations associated with streptomycin resistance in Mycobacterium tuberculosis were characterized in 78 streptomycin-resistant and 61 streptomycin-susceptible isolates recovered from patients living in the United States, South America, Europe, Africa, and Asia. Fifty-four percent of the 78 resistant organisms had missense mutations in codon 43 of rpsL resulting in a K-43-->R substitution. Mutations in codon 88 of rpsL were also identified in four Asian isolates.
Sreevatsan
S
S
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
Pan
X
X
Stockbauer
K E
KE
Williams
D L
DL
Kreiswirth
B N
BN
Musser
J M
JM
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017385:Sequence Homology; D013307:Streptomycin / Q000494:pharmacology*
D000904:Antibiotics, Antitubercular; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin