title: Peptides derived from the lectin domain of selectin adhesion molecules inhibit leukocyte rolling in vivo.
abstract: OBJECTIVE
  The selectins are a family of adhesion molecules that mediate leukocyte rolling, a prerequisite for their later firm adhesion and migration to sites of inflammation. The N-terminal lectin domain of selectins is important for Ca(2+)-dependent binding to oligosaccharide ligands. We set out to study the effect of peptides corresponding to residues 11-20, 23-30, 36-50, 54-63, 70-79 and 109-118 (counting from the N-terminus of the mature proteins) of the lectin domain of human L-, P- and E-selectins on leukocyte rolling in vivo.
  
  
  METHODS
  Peptides were applied by local intravascular microinfusion via a glass micropipette into rat mesenteric venules. Visibly rolling cells were counted off-line and compared with rolling cells counted during control periods.
  
  
  RESULTS
  Peptides corresponding to residues 70-79 of P-selectin and 11-20 of L-selectin reduced leukocyte rolling flux in rat mesenteric venules to less than 30% of that measured during control infusion. Peptides corresponding to residues 109-118 of P-selectin, 54-63 of L-selectin and 23-30 of E-selectin also reduced leukocyte rolling flux, although to a lesser degree.
  
  
  CONCLUSIONS
  We have shown that small peptides based on the lectin domain of all three selectins can be effective inhibitors of leukocyte rolling in vivo.
authors.affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Norman
authors.forename: C
authors.initials: C
authors.lastname: Scheding
authors.forename: E J
authors.initials: EJ
authors.lastname: Kunkel
authors.forename: G A
authors.initials: GA
authors.lastname: Heavner
authors.forename: K
authors.initials: K
authors.lastname: Ley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019040:E-Selectin / Q000737:chemistry; D005260:Female; D019041:L-Selectin / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D007962:Leukocytes / Q000187:drug effects*; D008969:Molecular Sequence Data; D019007:P-Selectin / Q000737:chemistry; D010455:Peptides / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D019087:Selectins / Q000737:chemistry*
chemical_list: D019040:E-Selectin; D037102:Lectins; D019007:P-Selectin; D010455:Peptides; D019087:Selectins; D019041:L-Selectin