Overexpression of Bcl-2 in a murine dopaminergic neuronal cell line leads to neurite outgrowth.
A dopaminergic neuronal cell line was established which stably expressed human Bcl-2 (MN9D/Bcl-2) or a control vector (MN9D/Neo). Overexpression of Bcl-2 cells led to robust neurite formation without cessation of cell division. Most markers of neuronal and/or dopaminergic maturation were unchanged although the synaptosomal associated protein, SNAP-25, was increased three-fold in MN9D/Bcl-2 cell lines. These data support a role for Bcl-2 in developmental processes at least during a certain stage(s) of neurogenesis.
Oh
Y J
YJ
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Swarzenski
B C
BC
O'Malley
K L
KL
D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology / Q000378:metabolism; D002460:Cell Line; D004298:Dopamine / Q000502:physiology*; D006801:Humans; D051379:Mice; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure; D009447:Neuroblastoma / Q000201:enzymology / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D014162:Transfection; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine