Sequence-specific binding and cleavage of duplex DNA by a radioiodinated, intercalator-linked, triplex-forming oligonucleotide.
Applications of oligodeoxynucleotides to modulate gene expression have been the subject of much recent research. We have sought to develop a method to permanently inactivate a gene, or potentially kill cells containing abnormal genes. In this report, we show that a DNA intercalator conjugated to a triplex-forming oligonucleotide can be labeled with an Auger electron emitting radioisotope, can cleave its duplex DNA target, and can specifically bind the target sequence contained in a total of 10 kilobases of irrelevant DNA.
Orson
F M
FM
Veterans Affairs Medical Center Research Center on AIDS and HIV Infections, Houston, TX, USA.
McShan
W M
WM
Kinsey
B M
BM
D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D013379:Substrate Specificity
D007364:Intercalating Agents; D007457:Iodine Radioisotopes; D009841:Oligonucleotides; D004247:DNA