Neurotransmitter positron emission tomographic-studies in adults with phenylketonuria, a pilot study.
Patients with phenylketonuria (PKU) may suffer from cognitive and neurological deficits which are related to reduced intracerebral concentrations of catecholamines. The function of phenylalanine (Phe) as an inhibitor of the uptake of the precursor amino acid tyrosine (Tyr) through the blood-brain barrier as well as an inhibitor of the expression of dopamine receptors in the brain is under investigation. Positron emission tomography (PET) is a method for quantitatively determining biochemical and physiological processes in vivo. In the current pilot study, L-[1-11C]-Tyr and 18F-fluoro-ethyl-spiperone (FESP) have been used. The metabolic pathway of carboxylic labelled Tyr is mainly incorporation into protein. From the measured tissue and plasma activity as a function of time in combination with a compartimental model the Protein Synthesis Rate (PSR) for Tyr can be calculated. FESP is a ligand which binds irreversibly to the dopamine D2-receptor and has also a low non specific binding, although affinity to the serotonin receptor has been described. The ratio of FESP concentration in striatum and in cerebellum is a measure of the receptor status in vivo. In patients with plasma Phe levels above the maximum therapeutic concentration (> 700 mumol/l) the PSR for Tyr was decreased as compared to controls and patients with plasma Phe concentrations within the therapeutic range, indicating a decreased availability of Tyr for neurotransmitter synthesis, and hence explaining the reduced cerebral concentration of catecholamines.
Paans
A M
AM
PET Centre, Groningen University Hospital, The Netherlands.
Pruim
J
J
Smit
G P
GP
Visser
G
G
Willemsen
A T
AT
Ullrich
K
K
D000293:Adolescent; D000328:Adult; D001921:Brain / Q000000981:diagnostic imaging*; D002250:Carbon Radioisotopes; D005260:Female; D006801:Humans; D008297:Male; D010649:Phenylalanine / Q000097:blood; D010661:Phenylketonurias / Q000097:blood / Q000000981:diagnostic imaging*; D010865:Pilot Projects; D013134:Spiperone / Q000031:analogs & derivatives; D014055:Tomography, Emission-Computed; D014443:Tyrosine / Q000378:metabolism
D002250:Carbon Radioisotopes; C051175:3-N-(2-fluoroethyl)spiperone; D014443:Tyrosine; D010649:Phenylalanine; D013134:Spiperone