[On the origin of extra bands at MCT118 typing].
In order to probe the origin of extra bands that appear frequently in MCT118 typing, we examined the extra bands by Dual PCR, Asymmetric PCR and PCR-SSCP method. It is conceivable that two extra bands of 16-18 type are DNA heteroduplexes that result from 16 and 18 allele bands at mutual cross-annealing. The extra bands can easily take the higher-order structure due to a non-complimentary sequence of bases. Consequently, the distances that extra bands travel in electrophoresis depend on a gel composition. Because the extra bands can be distinguished from the original allele bands by electrophoresis using different gel composition, it will not become an obstacle at the typing.
Watanabe
G
G
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Umetsu
K
K
Suzuki
T
T
D002878:Chromosomes, Human, Pair 1; D004587:Electrophoresis, Agar Gel; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational