Cloning, sequencing, and characterization of LDH-C4 from a fox testis cDNA library.
A full-length cDNA encoding the sperm-specific enzyme lactate dehydrogenase-C4 was isolated from a fox testis cDNA expression library and sequenced. The deduced translated protein sequence was shown to be 86% identical to that of human LDH-C4. In the fox testis, mRNA encoding LDH-C4 was first detected in pachytene spermatocytes. The LDH-C4 protein monomer was identified in Western blots of sperm membrane extracts as having a molecular weight of approximately 35,000, consistent with the monomeric size of this subunit previously identified in sperm from other species. The LDH-C4 protein is localized on the sperm plasma membrane overlying the principal piece of the tail. Based on the available sequence data, we were able to identify an epitope within the N-terminal region of the LDH-C4 amino-acid sequence which when administered to female foxes is antigenic and produces antibodies capable of recognizing the native protein.
Bradley
M P
MP
Perth Zoological Gardens, South Perth, Western Australia, Australia.
Geelan
A
A
Leitch
V
V
Goldberg
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D005589:Foxes; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012621:Seasons; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology*
D018076:DNA, Complementary; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase