title: Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction.
abstract: We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (PFGE). The patterns of gel electrophoresis of the products of AP-PCR differed significantly according to differences in the concentration of Mg2+ and in pH. AP-PCR and PFGE was identical in their resolving power, as the two methods generated four different profiles and identified the same group of strains. The AP-PCR method constitutes an easy alternative to the well-established PFGE method.
authors.affiliation: First Department of Internal Medicine, Kagawa Medical School, Yamaguchi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyawaki
authors.forename: J
authors.initials: J
authors.lastname: Fujita
authors.forename: K
authors.initials: K
authors.lastname: Takigawa
authors.forename: K
authors.initials: K
authors.lastname: Negayama
authors.forename: Y
authors.initials: Y
authors.lastname: Yamagishi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaji
authors.forename: K
authors.initials: K
authors.lastname: Ouchi
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: K
authors.initials: K
authors.lastname: Kawanishi
authors.forename: J
authors.initials: J
authors.lastname: Takahara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D001483:Base Sequence; D019121:Burkholderia Infections / Q000453:epidemiology*; D016956:Burkholderia cepacia / Q000235:genetics; D003428:Cross Infection; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology*