AR4-2J cell line coexpresses dihydropyridine and omega-conotoxin sensitive Ca2+ channels.
For the first time, we have demonstrated in AR4-2J cells, an experimental model of azaserine-induced carcinoma in the rat exocrine pancreas, the co-expression of alpha 1 subunit of dihydropyridine-sensitive Ca2+ channel and the alpha 1 sub-unit of omega-conotoxin-sensitive Ca2+ channel RNA messengers which share homologous sequences with, respectively, rbC II and rbB I sub-types described in the rat brain. These two types of voltage-dependent Ca2+ channels which are functionally expressed, emphasize the acquisition during carcinogenesis of neuroendocrine features of AR4-2J cells. Additionally, using antisense phosphorothioate oligodeoxynucleotide, we demonstrated clearly the involvement of dihydropyridine-sensitive Ca2+ channels in the control of AR4-2J cell proliferation.
Bertrand
V
V
INSERM U 151, Institut Fédératif de Recherches Louis Bugnard, CHU Rangueil, Toulouse, France.
Bastié
M J
MJ
Bouisson
M
M
Vaysse
N
N
Pradayrol
L
L
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D016375:Antisense Elements (Genetics) / Q000235:genetics; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism*; D002277:Carcinoma / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D004095:Dihydropyridines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D008978:Mollusk Venoms / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D051381:Rats; D014407:Tumor Cells, Cultured
D016375:Antisense Elements (Genetics); D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D017931:DNA Primers; D004095:Dihydropyridines; D008978:Mollusk Venoms; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium