title: Renal effects of metabolic acidosis in the normal rat.
abstract: Metabolic acidosis causes renal growth and proteinuria, and may contribute to the progression of CRF. This study assessed the effects of HCI-induced acidosis on the structure and function of normal kidneys. Acidosis was induced in 12 rats by dietary HCl. After 2 weeks, acidotic animals had higher kidney/body weight ratios (0.47 +/- 0.10 vs. 0.35 +/- 0.10 g%, p < 0.001) and higher kidney protein content (123 +/- 3 vs. 111 +/- 4 mg/kidney, p < 0.05) than controls, but tubular nuclear densities were lower, suggesting tubular hypertrophy. Acidotic animals developed tubular proteinuria (16.4 +/- 2.6 mg/day after 2 weeks of acidosis vs. 2.9 +/- 0.3 mg/day at baseline; p < 0.001), and the pattern of immunohistochemical staining for Tamm-Horsfall protein suggested tubular injury. These data suggest that a tubulotoxic effect of metabolic acidosis may contribute to the progression of CRF.
authors.affiliation: Department of Nephrology, Leicester General Hospital, UK.
authors.forename: D
authors.initials: D
authors.lastname: Throssell
authors.forename: K P
authors.initials: KP
authors.lastname: Harris
authors.forename: A
authors.initials: A
authors.lastname: Bevington
authors.forename: P N
authors.initials: PN
authors.lastname: Furness
authors.forename: A J
authors.initials: AJ
authors.lastname: Howie
authors.forename: J
authors.initials: J
authors.lastname: Walls
mesh_terms: D000138:Acidosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001784:Blood Gas Analysis; D001835:Body Weight / Q000502:physiology; D005260:Female; D006851:Hydrochloric Acid; D006984:Hypertrophy; D007074:Immunoglobulin G / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism; D009088:Mucoproteins / Q000378:metabolism / Q000652:urine; D009929:Organ Size / Q000502:physiology; D011507:Proteinuria / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D058949:Uromodulin
chemical_list: D007074:Immunoglobulin G; D009088:Mucoproteins; C548614:Umod protein, rat; D058949:Uromodulin; D006851:Hydrochloric Acid