A sensitive RNase protection assay using 33P labeled antisense riboprobes.
This article presents for the first time a modified protocol for RNase protection analysis that allows the substitution of 32P with 33P without loss of the high sensitivity of this method achieved with 32P. With this protocol, we were able to detect at least 1 pg of specific mRNA. In the RNase protection analysis 33P labeled riboprobes are more advantageous with regard to an easier handling and better resolution.
Meisinger
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Institute of Anatomy, University of Freiburg, PO Box 111, Germany.
Grothe
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D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D016716:PC12 Cells; D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D012260:Ribonucleases