title: Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose.
abstract: Fluorescence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.
authors.affiliation: University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Schwinefus
authors.forename: S C
authors.initials: SC
authors.lastname: Wang
authors.forename: R W
authors.initials: RW
authors.lastname: Hammond
authors.forename: M D
authors.initials: MD
authors.lastname: Morris
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D004586:Electrophoresis / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D017900:Myoviridae / Q000235:genetics; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence
chemical_list: D004279:DNA, Viral; D002482:Cellulose; C002283:hydroxyethylcellulose