Effect of tripeptide-copper complexes on the process of skin wound healing and on cultured fibroblasts.
The effects of Gly-His-Lys-Cu and of three synthetic analogues (I, II and III) on wound healing of the guinea-pig dorsal skin, as well as on cultured fibroblasts, were examined. Gly-His-Lys-Cu and peptide I-Cu were tested in vivo. Hydroxyproline, proteins, DNA and semicarbazide-sensitive amine oxidase, with a high affinity for benzylamine, were measured, and the histology of the wounds was observed after staining with hematoxylin/eosin. Another set of wounds was treated in parallel with equivalent amounts of copper acetate. Gly-His-Lys-Cu and the analogues caused a decrease of the activity of semicarbazide-sensitive amine oxidase, with a high affinity for benzylamine, 4-8 days after surgery, followed by an increase on day 11 that was higher than in the control group. No significant difference was found between the two peptides. A slower reorganization of the skin and a delayed activation of fibroblasts are the main effects observed with these peptides-Cu complexes. Preliminary studies on cultured fibroblasts were monitored to see whether these peptides had a direct effect on fibroblasts. The products studied at a concentration of 10(-7) M, decreased cell reproduction and increased collagen expression.
Buffoni
F
F
Department of Pharmacology, University of Florence, Firenze, Italy.
Pino
R
R
Dal Pozzo
A
A
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002478:Cells, Cultured; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006133:Growth Substances / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D006168:Guinea Pigs; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009842:Oligopeptides / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D013194:Staining and Labeling; D014945:Wound Healing / Q000187:drug effects*
D002273:Carcinogens; D006133:Growth Substances; D009842:Oligopeptides; D009942:Organometallic Compounds; D011506:Proteins; C015092:cupric acetate; C020905:glycyl-histidyl-lysine; D003300:Copper; D004247:DNA; D006631:Amine Oxidase (Copper-Containing); D006909:Hydroxyproline