[Role of TGF-beta and PDGF on the pathogenesis of pulmonary fibrosis--analysis by in vivo gene transfer].
To elucidate the relevance of transforming growth factor (TGF)-beta 1 and platelet-derived growth factor (PDGF)-B to the pathogenesis of pulmonary fibrosis, we introduced each of these expression vectors via trachea into Wistar rat to overexpress them locally in the lungs by hemagglutinating virus of Japan (HVJ)-liposome method. The TGF-beta 1 gene induced significant proliferatin of fibroblasts and deposition of collagen fibrils with mild cellular infiltration. The PDGF-B gene induced mild fibrotic changes with some cellular infiltration. These findings suggest that both factors may be very closely relevant to the pathogenesis of lung fibrosis.
Yoshida
M
M
Internal Medicine III, Osaka University Medical School.
Hayashi
S
S
D000818:Animals; D018014:Gene Transfer Techniques; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000502:physiology*; D011658:Pulmonary Fibrosis / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta