title: Molecular analysis of allergenic proteins in bovine dander.
abstract: An analytic procedure was established to characterize bovine dander proteins with allergenic properties. The proteins from dander extract were separated by size-exclusion gel filtration, and the fractions were studied with SDS-PAGE followed by immunoblotting. An 11-kDa allergen was found in the same gel filtration fractions as 20- and 22-kDa allergens, and this suggests that the 11-kDa allergen is a dimer in its native form. Our method also detected two separate 22-kDa allergens. The primary structure of the major bovine dander allergen (BDA20) was also studied. A protein sequencer was used to determine the amino acid sequences of enzymatically cleaved peptides. The homology searches revealed that BDA20 is not a previously known bovine protein.
authors.affiliation: Department of Clinical Microbiology, University of Kuopio, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Rautiainen
authors.forename: J
authors.initials: J
authors.lastname: Pentikäinen
authors.forename: M
authors.initials: M
authors.lastname: Rytkönen
authors.forename: A
authors.initials: A
authors.lastname: Linnala-Kankkunen
authors.forename: J
authors.initials: J
authors.lastname: Pelkonen
authors.forename: T
authors.initials: T
authors.lastname: Virtanen
authors.forename: R
authors.initials: R
authors.lastname: Mäntyjärvi
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D012867:Skin / Q000276:immunology*
chemical_list: D000485:Allergens; D011506:Proteins