title: Amino acid sequence of catechol 1,2-dioxygenase (pyrocatechase) isozyme alpha alpha from Pseudomonas arvilla C-1.
abstract: Catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of catechol to form cis,cis-muconic acid, and contains a ferric form of iron as its sole cofactor. Pseudomonas arvilla C-1 contains three isozymes of the enzyme, alpha alpha, alpha beta, and beta beta [C. Nakai et al. (1990) J. Biol. Chem. 265, 660-665]. We have determined the amino acid sequence of the alpha alpha isozyme by direct analysis. The sequence shared 77% homology with isozyme beta beta, and had conserved tyrosyl and histidyl residues which are thought to be involved in the binding of ferric ion.
authors.affiliation: Department of Biochemistry, Shiga University of Medical Science, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Nakai
authors.forename: G
authors.initials: G
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Kagamiyama
authors.forename: M
authors.initials: M
authors.lastname: Nozaki
mesh_terms: D000595:Amino Acid Sequence; D050548:Catechol 1,2-Dioxygenase; D049308:Dioxygenases; D007527:Isoenzymes / Q000737:chemistry*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology; D016415:Sequence Alignment
chemical_list: D007527:Isoenzymes; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase