Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction.
We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (PFGE). The patterns of gel electrophoresis of the products of AP-PCR differed significantly according to differences in the concentration of Mg2+ and in pH. AP-PCR and PFGE was identical in their resolving power, as the two methods generated four different profiles and identified the same group of strains. The AP-PCR method constitutes an easy alternative to the well-established PFGE method.
Miyawaki
H
H
First Department of Internal Medicine, Kagawa Medical School, Yamaguchi, Japan.
Fujita
J
J
Takigawa
K
K
Negayama
K
K
Yamagishi
Y
Y
Yamaji
Y
Y
Ouchi
K
K
Nakazawa
T
T
Kawanishi
K
K
Takahara
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D001483:Base Sequence; D019121:Burkholderia Infections / Q000453:epidemiology*; D016956:Burkholderia cepacia / Q000235:genetics; D003428:Cross Infection; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology*