Novel mouse embryonic renal marker gene products differentially expressed during kidney development.
Investigators approaching the problem of renal organogenesis have been hampered by a paucity of suitable molecular markers that specify distinct developmental phenotypes. To identify such markers, differential display-polymerase chain reaction (DD-PCR) was used to survey the temporal pattern of gene expression in mouse kidney at 11.5, 13.5, 15.5, and 17.5 days after conception and in the adult kidney. Twenty-two differentially expressed amplification products were identified, isolated, and sequenced. Seventeen clones showed no significant similarity with previously reported nucleotide sequences: two were similar to two housekeeping gene products, and three were similar to human or rat expressed sequence tags. To confirm the differential expression patterns observed by DD-PCR, semiquantitative reverse transcription-PCR was performed using sequence-specific oligonucleotide primers. Nineteen of 22 clones were differentially expressed during kidney development [mouse embryonic renal marker (MERM) sequences 1-19]. The value of MERMs as developmental markers was further assessed in mouse metanephric organ culture, where the pattern of MERM transcript expression mimicked that observed in vivo. Therefore, the DD-PCR method permitted development of a panel of marker sequences that can be used to characterize renal developmental processes and that may allow the identification of novel, functionally relevant gene products.
Kretzler
M
M
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0676, USA.
Fan
G
G
Rose
D
D
Arend
L J
LJ
Briggs
J P
JP
Holzman
L B
LB
D000818:Animals; D015415:Biomarkers; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000196:embryology* / Q000378:metabolism; D051379:Mice / Q000196:embryology; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
D015415:Biomarkers; D011506:Proteins