Cloning of a Plasmodium falciparum gene related to the human 60-kDa heat shock protein.
We have identified a gene encoding the 60 kDa heat shock protein (hsp60) from a Plasmodium falciparum blood stage cDNA library. The deduced protein sequence encodes for a polypeptide of 577 amino acids with a calculated molecular weight of 62158 Da. The primary structure of P. falciparum hsp60 contains a putative mitochondrial targeting peptide at its amino-terminus and a GGM motif at its carboxyl-terminus. The overall structure exhibits strong conservation (approximately 50%) to the hsp60 from human and other eukaryotes, but only low homology (< 30%) to a recently reported P. falciparum chaperonin 60 gene. The P. falciparum hsp60 gene is located on chromosome 10. During heat shock, the level of hsp60 transcript in blood stage parasites increases significantly and its accumulation correlates with the duration of the induction.
Syin
C
C
Laboratory of Parasitic Biology and Biochemistry, Food and Drug Administration, Rockville, MD 20852-1448, USA. syin@al.cber.fda.gov
Goldman
N D
ND
D000595:Amino Acid Sequence; D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005057:Eukaryotic Cells; D017125:Genes, Protozoan; D018869:Heat-Shock Response; D006801:Humans; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D011387:Prokaryotic Cells; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018834:Chaperonin 60; D018076:DNA, Complementary