Defect in export and synthesis of the periplasmic galactose receptor MglB in dnaK mutants of Escherichia coli, and decreased stability of the mglB mRNA.
The high-affinity galactose permease, which comprises the periplasmic galactose receptor MglB, the membrane translocator MglC and the membrane-associated ATPase MglA, displayed a reduced activity in a dnaK temperature-sensitive mutant of Escherichia coli. This reduced transport activity correlated with a reduction in the quantity of MglB. At 42 degrees C, an accumulation of pre-MglB in the dnaK temperature-sensitive mutant reflected a defect in MglB export. In addition, an accumulation of pre-MglB in secB, secA and secY mutants suggested that SecB and the Sec translocase are also involved in export of the periplasmic galactose receptor. At 30 degrees C, there was no accumulation of pre-MglB in the dnaK mutant, but there was still a decreased amount of MglB in the periplasm. The reduction in MglB expression was not the result of a decrease in its stability, nor was it the result of a general defect in translation or transcription, since the MglA protein (which is expressed from the same operon as MglB) was synthesized in normal amounts. Two mRNAs are implicated in the expression of the mgl genes, a polycistronic mglBAC mRNA, and a more stable and more abundant mglB mRNA, produced by 3'-5' degradation of the mglBAC mRNA (R. W. Hogg, C. Voelker & I. von Carlowitz, 1991, Mol Gen Genet 229, 453-459). The mglB mRNA is protected against exonucleases by a REP (Repetitive Extragenic Palindrome) sequence located at its 3' extremity, which is responsible for the higher expression of MglB compared to MglA and MglC. The decreased MglB expression in the dnaK mutant at 30 degrees C in the present work correlated with a reduced stability of the mglB mRNA, which may have resulted from a defective stabilization by the REP sequence, or from a defect in translation of the mglB gene.
el Yaagoubi
A
A
Génétique et Biochimie, Institut Jacques Monod, Université Paris, France.
Kohiyama
M
M
Richarme
G
G
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001692:Biological Transport; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005690:Galactose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013696:Temperature; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C489579:MglC protein, E coli; C075401:MglC protein, bacteria; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000069816:SEC Translocation Channels; C039707:galactose receptor; C023550:galactose-binding protein; C071728:MglA protein, Myxococcus xanthus; D000251:Adenosine Triphosphatases; C063842:dnaK protein, E coli; D000081416:SecA Proteins; D005690:Galactose