title: In situ nick end-labeling detects necrosis of hippocampal pyramidal cells induced by kainic acid.
abstract: It is believed that in situ nick end-labeling (ISNEL) is an easy and selective method for detecting apoptosis in situ. To test whether ISNEL selectively detects apoptosis but not necrosis, we investigated the kainic acid (KA)-induced neuronal death with ISNEL, comparing with the results of gel electrophoresis and electron microscopy. Many degenerating neurons (ca. 50%) in the hippocampal CA1 area and amygdaloid complex were intensely stained with ISNEL 1-3 days after intraperitoneal injection of KA. Although most of the ISNEL-positive neurons displayed a pathological feature of necrosis, a small number of them displayed apoptosis-like changes when examined by electron microscopic observation. It should be noteworthy that ISNEL recognizes at least a certain form of necrosis and is not selective for apoptosis.
authors.affiliation: Department of Neurology, School of Medicine, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nishiyama
authors.forename: S
authors.initials: S
authors.lastname: Kwak
authors.forename: S
authors.initials: S
authors.lastname: Takekoshi
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Kanazawa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007608:Kainic Acid