title: Ultrastructural localization of the intercellular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes.
abstract: BACKGROUND
  The high endothelial venules (HEV) in the lymph nodes are essential for lymphocyte recirculation. As a first step, the HEV surface interacts with lymphocytes through adhesion molecules. It is important to know where adhesion molecules are expressed on the surface ultrastructure and how these structures interact with lymphocytes.
  
  
  METHODS
  To demonstrate the ultrastructural mechanism of interaction between the HEV surface and lymphocytes through the intercellular adhesion molecule (ICAM-1), rat mesenteric lymph nodes were perfused through the superior mesenteric artery with the primary antibody (antirat ICAM-1 antibody) and secondary antibody (antimouse IgG coupled to 15 nm gold particles), which were diluted with hypothermic University of Wisconsin (UW) solution. After the immunoreaction, we analyzed the HEV three-dimensionally and quantitatively using immunoscanning electron microscopy (ISEM) combined with transmission electron microscopy (TEM).
  
  
  RESULTS
  HEV expressed ICAM-1 in a 5-30-fold higher concentration than other vessels. Its distribution was extensive over the luminal surface of the cell down to the junctional area. The endothelial surface of HEV undulated to form branched microfolds, along which ICAM-1 was expressed. Cytoplasmic processes of lymphocytes were seen in microfurrows between microfolds and adhered to the sides of the folds.
  
  
  CONCLUSIONS
  These observations imply that the microfolds expressing ICAM-1 and microfurrows are specific ultrastructural features for trapping lymphocytes, thus initiating lymphocyte emigration into the lymph node parenchyma.
authors.affiliation: Department of Anatomy, Yamagata University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Okouchi
authors.forename: H J
authors.initials: HJ
authors.lastname: Rothkötter
authors.forename: R
authors.initials: R
authors.lastname: Pabst
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure*; D017612:Gold Compounds; D018799:Intercellular Adhesion Molecule-1 / Q000648:ultrastructure*; D008198:Lymph Nodes / Q000098:blood supply* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013194:Staining and Labeling; D014699:Venules / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D017612:Gold Compounds; D018799:Intercellular Adhesion Molecule-1