title: Interleukin 1 beta, interleukin 6, tumor necrosis factor alpha, and interleukin 10 responses in peripheral blood mononuclear cells of cynomolgus macaques during acute infection with SIVmac251.
abstract: HIV infection ultimately leads to AIDS, despite the immune responses elicited soon after infection. In addition to the observed changes in lymphoid cell subsets, alteration of the cytokine network most likely accompanies and/or contributes to the lack of protective immune responses. In an attempt to delineate the early events in the immune response to lentivirus infection, we have sequentially monitored levels of proinflammatory (IL-1 beta, IL-6, and TNF-alpha) and antiinflammatory (IL-10) cytokine mRNAs in PBMCs of cynomolgus macaques during primary SIVmac infection. Eight monkeys were infected i.v. with 4 AID50 of cell-free SIVmac251. All monkeys seroconverted between days 16 and 21 postinfection (p.i.), and had detectable peripheral viremia. The viral load peaked between days 12 and 16 p.i., and fell sharply thereafter. A marked increase in the expression of IL-6 mRNA was observed in all macaques during the first weeks following infection. An increase in the levels of expression of IL-1 beta, TNF-alpha, and IL-10 mRNA was also determined in six, six, and five of the eight monkeys, respectively. While IL-6, TNF-alpha, and IL-10 increased transiently, increased levels of IL-1 beta mRNA expression were sustained over 44 days in most monkeys.
authors.affiliation: Commissariat à l'Energie Atomique, Institut Paris-Sud sur les Cytokines, Fontenay aux Roses, France.
authors.forename: O
authors.initials: O
authors.lastname: Benveniste
authors.forename: B
authors.initials: B
authors.lastname: Vaslin
authors.forename: R
authors.initials: R
authors.lastname: Le Grand
authors.forename: P
authors.initials: P
authors.lastname: Fouchet
authors.forename: V
authors.initials: V
authors.lastname: Omessa
authors.forename: F
authors.initials: F
authors.lastname: Theodoro
authors.forename: P
authors.initials: P
authors.lastname: Fretier
authors.forename: P
authors.initials: P
authors.lastname: Clayette
authors.forename: F
authors.initials: F
authors.lastname: Boussin
authors.forename: D
authors.initials: D
authors.lastname: Dormont
mesh_terms: D000208:Acute Disease; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D017931:DNA Primers; D005500:Follow-Up Studies; D015683:Gene Products, gag / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D007074:Immunoglobulin G; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10