title: Clustered organization and conservation of the Xiphophorus maculatus D locus, which includes two distinct gene sequences.
abstract: Genomic organization and chromosomal localization of a previously uncharacterized D (Donor) locus in Xiphophorus and Poecilia species was investigated using fluorescence in situ hybridization (FISH) and Southern blot analysis. Part of this region is thought to be involved in the recombination event leading to formation of the Xmrk oncogene and it has recently been shown that this locus includes two different genes, one with high homology to a zinc finger protein of the Krüppel type, and the other an unknown gene with high similarity to a Caenorhabditis elegans gene. FISH to Xiphophorus chromosomes revealed that these two unrelated genes are closely linked and clustered at a unique chromosomal site. Southern blot hybridization patterns suggest that these genes exist in the genome as multiple copies. Furthermore, similar genomic organization profiles seem to prevail among other related fish. In particular, our FISH experiments reveal the existence of a conserved homologous chromosomal segment harboring the zinc finger protein sequence in several poeciliid fish.
authors.affiliation: Department of Human Genetics, University of Würzburg, Biocenter, Am Hubland, D-97074 Würzburg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Nanda
authors.forename: S
authors.initials: S
authors.lastname: Weis
authors.forename: D
authors.initials: D
authors.lastname: Förnzler
authors.forename: J
authors.initials: J
authors.lastname: Altschmied
authors.forename: M
authors.initials: M
authors.lastname: Schartl
authors.forename: M
authors.initials: M
authors.lastname: Schmid
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D019143:Evolution, Molecular; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny; D011036:Poecilia / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics*