Purification of the monoclonal heparin antibody H-1.18.
An antibody of the (immunoglobulin) IgG1 subclass against heparin was purified. Here we report on the purification of the heparin antibody. Ammonium sulfate precipitation was performed and showed a high purity of the precipitate. In the heparin radioimmunoassay it showed a high heparin binding. Capillary electrophoresis showed that albumin and other proteins were separated from the heparin antibody. The purification method allowed a large scale production of the heparin antibody.
Malsch
R
R
First Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Germany.
Mrotzek
T
T
Huhle
G
G
Harenberg
J
J
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D019075:Electrophoresis, Capillary; D006493:Heparin / Q000276:immunology*; D006495:Heparin, Low-Molecular-Weight / Q000032:analysis; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007457:Iodine Radioisotopes; D051379:Mice; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000276:immunology; D014439:Tyramine / Q000032:analysis
D000911:Antibodies, Monoclonal; D006495:Heparin, Low-Molecular-Weight; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A; D006493:Heparin; D000645:Ammonium Sulfate; D014439:Tyramine