Central V1 AVP receptors are involved in cardiovascular adaptation to hypovolemia in WKY but not in SHR.
The present study was designed to determine the role of centrally released arginine vasopressin (AVP) in cardiovascular adaptation to hypotensive hypovolemia in conscious normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). Three groups of experiments were performed on WKY and SHR chronically implanted with lateral cerebral ventricle (LCV) cannulas and with femoral artery catheters. Mean arterial pressure (MAP) and heart rate (HR) were monitored before and after arterial bleeding (1.3% body weight) performed during LCV infusion 1) artificial cerebrospinal fluid (control), 2) V1 AVP-receptor antagonists {[d(Et2)Tyr(Me)]DAVP, 5 ng/min}, and 3) V2 AVP-receptor antagonists {[d(CH2)5-D-Ile2, Ile4, AlaNH2]AVP, 5 ng/min}. In control experiments hemorrhage caused similar significant decreases of MAP in both strains and bradycardia in WKY. Blockade of central V1 AVP receptors abolished hemorrhagic bradycardia and significantly reduced hypotension in WKY, with no effect on HR and MAP responses to hypovolemia in SHR. Neither in WKY nor in SHR were the cardiovascular responses to hemorrhage altered by blockade of central V2 receptors. The results suggest that the central V1 AVP system plays a significant role in eliciting hypovolemic bradycardia and hypotension in WKY and that this function is significantly impaired in SHR.
Budzikowski
A S
AS
Department of Clinical and Applied Physiology, Warsaw Medical Academy, Poland.
Paczwa
P
P
Szczepańska-Sadowska
E
E
D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001794:Blood Pressure / Q000187:drug effects; D001810:Blood Volume; D001921:Brain / Q000378:metabolism*; D002319:Cardiovascular System / Q000503:physiopathology*; D002555:Cerebrospinal Fluid; D006339:Heart Rate / Q000187:drug effects; D007276:Injections, Intraventricular; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*; D011921:Rats, Inbred WKY / Q000502:physiology*; D017483:Receptors, Vasopressin / Q000502:physiology*
D065092:Antidiuretic Hormone Receptor Antagonists; D017483:Receptors, Vasopressin