Comparative genomic hybridization analysis of archival formalin-fixed paraffin-embedded uveal melanomas.
Comparative genomic hybridization (CGH) was used to analyze seven autologous uveal melanomas with both formalin-fixed, paraffin-embedded and fresh-frozen specimens. In addition, DNA from two archival formalin-fixed tumors more than 45 years old were also analyzed. The most frequent genetic changes were loss of chromosome 3; increase in copy number of 6p and loss of 6q; and increase in copy number of 8q. A comparison of CGH data from the fresh-frozen tumors and their autologous formalin-fixed tumors revealed a correlation coefficient of 0.83. Comparative genomic hybridization (CGH) analysis of 45-year-old specimens identified genetic changes similar to those found in more recent tumors including loss of chromosome 3 and increase in copy numbers of 6p and 8q. The results indicate that there is a good agreement between data obtained from formalin-fixed and fresh-frozen specimens using CGH. Furthermore, the results demonstrate the applicability of this technique in analyzing archival formalin-fixed tumors that were previously not accessible to cytogenetic analysis.
Ghazvini
S
S
Department of Ophthalmology, University of California, San Francisco 94143, USA.
Char
D H
DH
Kroll
S
S
Waldman
F M
FM
Pinkel
D
D
D000704:Analysis of Variance; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D002898:Chromosomes, Human, Pair 8; D004273:DNA, Neoplasm / Q000032:analysis; D005557:Formaldehyde; D005615:Freezing; D006652:Histological Techniques; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D014604:Uveal Neoplasms / Q000235:genetics* / Q000473:pathology*
D004273:DNA, Neoplasm; D005557:Formaldehyde