title: Chromosomal localization of homeobox genes and associated markers on porcine chromosomes 3, 5, 12, 15, 16 and 18: comparative mapping study with human and mouse.
abstract: Four homeobox genes that belong to the four homeobox gene clusters known in mammals have been regionally assigned to four distinct porcine chromosomes in conserved regions between human and pig. HOXA11, HOXB6, HOXC8, and HOXD4 genes were mapped by radioactive in situ hybridization to porcine Chromosomes (Chrs) 18q21-24 (with a secondary signal in 16q14-21), 12p11-12, 5p11-12, and 15q22-23 respectively. Besides, we have also revealed the presence of a porcine homeobox (pig Hbx24) which, although showing DNA sequence homology with a mouse gene of HOXB cluster, was located on porcine Chr 3 (3p14-13) outside the Hox clusters. To support the identity of the homeobox gene clusters analyzed and in the light of the high sequence similarity among homeobox genes, we also localized markers known to be mapped near each Hox cluster in human. In this way, four genes were also mapped in pig: GAPD (5q12-21), GAD1 (15q21-22), INHBA (18q24), and IGFBP3 (18q24). Mapping of HOXA11, INHBA, and IGFBP3 on pig Chr 18 constitutes the first assignments of genes on this small chromosome. These new localizations extend the information on the conservation of four human chromosomal regions in the pig genome.
authors.affiliation: Laboratoire de Genetique Cellulaire, Centre INRA Toulouse, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Lahbib-Mansais
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: P
authors.initials: P
authors.lastname: Pinton
authors.forename: J
authors.initials: J
authors.lastname: Gellin
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005801:Genes, Homeobox / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D013552:Swine / Q000235:genetics*
chemical_list: D005819:Genetic Markers