Effects of indole-3-acetic acid on croton oil- and arachidonic acid-induced mouse ear edema.
The indole-3-acetic acid (IAA) is a plant growth hormone (auxin) being considered as a tryptophan metabolite in animals. The main purpose of this work was to verify IAA's topical anti-inflammatory action using croton oil- or arachidonic acid-induced mouse ear edema, in comparison to known anti-inflammatory agents. IAA antioxidant activity was also verified by measuring the inhibition of brain homogenate lipid peroxidation with the thiobarbituric acid reactive substances (TBARS) test. IAA inhibited the action of both croton oil-and arachidonic acid-induced edema in a dose-dependent manner (4.0 mumoles IAA inhibited 75.8% in croton oil and 82.5% in arachidonic acid induced ear edema). Both IAA (5.3 mM) and indomethacin (8.0 mM) inhibited TBARS formation. Data suggest that IAA exhibits antiinflammatory effect possibly by its anti-oxidant activity.
Jones
L H
LH
Department of Physiology, University of São Paulo, Brazil.
Abdalla
D S
DS
Freitas
J C
JC
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000494:pharmacology; D003436:Croton Oil / Q000494:pharmacology; D004487:Edema / Q000517:prevention & control; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D007210:Indoleacetic Acids; D016718:Arachidonic Acid; C030737:indoleacetic acid; D003436:Croton Oil; D007213:Indomethacin