title: Defective phorbol ester-stimulated secretion of beta-amyloid precursor protein from Alzheimer's disease fibroblasts.
abstract: The present study shows that cultured fibroblasts from sporadic Alzheimer's disease patients are deficient in protein kinase C-regulated secretion of amyloid precursor protein. In particular, Alzheimer fibroblasts show a reduced basal secretion and a reduced response at low concentrations of phorbol-12,13-dibutyrate, with an EC50 twofold higher than control fibroblasts. Furthermore, we observed that such defective regulation of the amyloid precursor secretion can possibly be correlated to a specific defect in protein kinase C alpha in fibroblasts from Alzheimer patients.
authors.affiliation: Institute of Pharmacological Sciences, Universita' di Milano, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bergamaschi
authors.forename: G
authors.initials: G
authors.lastname: Binetti
authors.forename: S
authors.initials: S
authors.lastname: Govoni
authors.forename: W C
authors.initials: WC
authors.lastname: Wetsel
authors.forename: F
authors.initials: F
authors.lastname: Battaini
authors.forename: M
authors.initials: M
authors.lastname: Trabucchi
authors.forename: A
authors.initials: A
authors.lastname: Bianchetti
authors.forename: M
authors.initials: M
authors.lastname: Racchi
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism* / Q000494:pharmacology*; D015153:Blotting, Western; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D016564:Amyloid beta-Protein Precursor; D007527:Isoenzymes; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C