The rat T-cell surface protein RT6 is associated with src family tyrosine kinases and generates an activation signal.
RT6 is a glycosyl-phosphatidylinositol-linked surface molecule present on most mature rat T-cells. RT6+ T-cells can prevent the expression of autoimmune diabetes in the BB rat, but the mechanism is unknown. Because cross-linking of other glycosyl-phosphatidylinositol-linked T-cell proteins is known to activate T-cells, we investigated the signaling properties of RT6. Antibody cross-linking of RT6 enhanced expression of the alpha subunit of the interleukin-2 (IL-2) receptor and potentiated the proliferation of rat T-cells cultured in the presence of phorbol ester plus recombinant IL-2 (rIL-2) and/or rIL-4. RT6 was found to coimmunoprecipitate with five tyrosine phosphorylated proteins including p60fyn and p56lck, members of the src tyrosine kinase family. Pretreatment of T-cells with phorbol ester increased the phosphorylation of proteins that coimmunoprecipitated with RT6, altered the electrophoretic mobility of several of these coimmunoprecipitated phosphoproteins, and increased the amount of p60fyn and p56lck coimmunoprecipitated with RT6. These data indicate that RT6-mediated signaling events may prime T-cells to respond to exogenous cytokines, suggesting a possible mechanism by which surface RT6 may influence T-cell function.
Rigby
M R
MR
Department of Medicine, University of Massachusetts Medical Center, Worcester, USA.
Bortell
R
R
Greiner
D L
DL
Czech
M P
MP
Klarlund
J K
JK
Mordes
J P
JP
Rossini
A A
AA
D036002:ADP Ribose Transferases; D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015153:Blotting, Western; D004247:DNA / Q000096:biosynthesis; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000503:physiopathology*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis / Q000502:physiology*; D010766:Phosphorylation; D051381:Rats; D011913:Rats, Inbred BB; D011920:Rats, Inbred WF; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D019061:src-Family Kinases / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D008562:Membrane Glycoproteins; D015375:Receptors, Interleukin-2; D004247:DNA; D036002:ADP Ribose Transferases; C090163:Art2b protein, rat; D019061:src-Family Kinases; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine