Rapid capillary electrophoretic analysis of human serum proteins: qualitative comparison with high-throughput agarose gel electrophoresis.
This study details the qualitative results from a comparative evaluation of agarose gel electrophoresis (AGE) and (CZE) as a screening procedure for monoclonal proteins in serum. Three hundred and five serum samples were analyzed by the two techniques; samples suspected of containing monoclonal proteins based on abnormalities observed with AGE or CZE were confirmed by immunoelectrophoresis and/or immunofixation. CZE separation conditions were simple (requiring only a bare silica capillary and 150 mM borate buffer, pH 10.0) and separation was complete in under 120 s. The results obtained by CZE were comparable or better than those obtained with AGE. Samples displaying "point-of-application" artifacts on AGE were not problematic by CZE analysis; an abnormal profile, due to the presence of a monoclonal protein, or a normal profile were clearly observable. The rapid analysis, excellent reproducibility, automation and relatively high throughput (approximately 90 samples per 8 h on a single instrument) sets the stage for CZE analysis of serum proteins to be used routinely in a clinical setting.
Clark
R
R
Department of Laboratory Medicine and Pathology, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Katzmann
J A
JA
Wiegert
E
E
Namyst-Goldberg
C
C
Sanders
L
L
Oda
R P
RP
Kyle
R A
RA
Landers
J P
JP
D000328:Adult; D000368:Aged; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D002624:Chemistry, Clinical / Q000191:economics / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods; D019075:Electrophoresis, Capillary / Q000191:economics / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009862:Online Systems / Q000295:instrumentation*; D015203:Reproducibility of Results; D018401:Sample Size; D013997:Time Factors
D001798:Blood Proteins