The isolation and some properties of the membrane-bound lactate dehydrogenase of Paracoccus denitrificans.
Membrane-bound lactate dehydrogenase was isolated from the cells of Paracoccus denitrificans by a combination of phase separation and chromatographic methods. It was NAD(+)-independent; phenazine methosulphate and 2,6-dichlorophenolindophenol could act as electron acceptors. It preferred D-lactate over the L-form (K(m) = 0.81 and 4.40 mM, respectively). Relative molecular weight estimations were 54000 +/- 3000 (electrophoresis) and 50000 +/- 5000 (gel chromatography) It was inhibited by thenoyltrifluoroacetone and diethyl pyrocarbonate, SH-reagents were without effect. No other lactate dehydrogenase activity was found in any subcellular fraction of cells grown on D,L-lactate, L-lactate, glucose or succinate.
Zboril
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Department of Biochemistry, Masaryk University, Brno, Czech Republic.
Wernerová
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D000096:Acetone / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D007525:Isoelectric Focusing; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010231:Paracoccus denitrificans / Q000201:enzymology*; D012402:Rotenone / Q000494:pharmacology; D012966:Sodium Cyanide / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013804:Thenoyltrifluoroacetone / Q000494:pharmacology
D001426:Bacterial Proteins; D002614:Chelating Agents; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D013439:Sulfhydryl Reagents; D012402:Rotenone; D000096:Acetone; D013804:Thenoyltrifluoroacetone; D000968:Antimycin A; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D004047:Diethyl Pyrocarbonate; D012966:Sodium Cyanide