title: A 2-year longitudinal study of elastase in human gingival crevicular fluid and periodontal attachment loss.
abstract: The purpose of this study is to determine whether gingival crevicular fluid (GCF) elastase total activity (TA) and concentration (EC) correlate with and predict progressive attachment loss (AL). 75 previously untreated patients with moderate periodontitis were recruited. GCF was collected from 16 molar and premolar mesiobuccal sites and probing attachment loss (PAL), probing depth (PPD), gingival index (GI), gingival bleeding index (GBI) and plaque index (Pl.I) were measured. PAL and PPD were measured with an electronic, constant pressure probe. Patients were given basic periodontal treatment prior to baseline when the above procedures were repeated. In addition, carefully localised radiographs were taken of the test teeth and repeated annually. Patients were seen at 3 months intervals to 2 years and the procedures were repeated. 119 AL sites were detected and 89 of these were rapid AL sites (RAL) and 30 were gradual AL sites (GAL). Elastase levels (TA & EC) at RAL sites were significantly higher (p < or = 0.0001) than paired control sites in the same patient at both the attachment loss time (ALT) and the prediction time (PT). The mean levels (TA & EC) over the study period at GAL sites were significantly higher (p < or = 0.0001) than paired control sites in the same patient. Using a critical value (CV) of 125 micronsU/30 s (TA) and 400 micronsU/micronsL (EC) in 2 x 2 contingency tables showed a sensitivity of 100% and specificity of 99.95% (TA) and a sensitivity of 100% and specificity of 99.91% (EC) at the PT with very similar values at the ALT. Patient level comparisons showed that the mean elastase levels (TA & EC) were significantly higher (p < or = 0.0001) at RAL and GAL sites than non-attachment loss (NAL) sites in AL patients and that the mean levels were significantly higher (p < or = 0.0001) in AL patients than NAL patients. All these results indicate that these CVs for GCF elastase activity may serve as a predictors of future attachment loss.
authors.affiliation: Periodontal Department, King's College School of Medicine & Dentistry, Denmark Hill, London, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Eley
authors.forename: S W
authors.initials: SW
authors.lastname: Cox
mesh_terms: D000328:Adult; D004796:Clinical Enzyme Tests; D018450:Disease Progression; D005260:Female; D005455:Fluorescent Antibody Technique; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D010196:Pancreatic Elastase / Q000032:analysis*; D017622:Periodontal Attachment Loss / Q000175:diagnosis* / Q000201:enzymology; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D010196:Pancreatic Elastase; D019272:Leukocyte Elastase