[Partial purification of endogenous growth factor(s) of retinal pigment epithelial cells from neural retina].
We attempted to purify endogenous growth factors of chick embryonal retinal pigment epithelial cells from bovine neural retina. Ammonium sulfate precipitation, gel filtration, and anion exchange column chromatography were used. Though only partially purification, bovine retinal extract had two effects, growth and transformation, on chick embryonal pigment epithelial cells. We think that these purified factors may be novel from the estimation of their molecular weight by gel filtration.
Katoh
K
K
Department of Ophthalmology, Tohoku University School of Medicine.
Ishiguro
S
S
Tamai
M
M
D000818:Animals; D002417:Cattle; D002642:Chick Embryo; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D012160:Retina / Q000737:chemistry*
D006133:Growth Substances