Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter.
Following the identification of a M. tuberculosis phosphate transporter belonging to the superfamily of ABC transporters, we report on the cloning and sequencing of two additional genes, called pstS-3 and pstC-2, encoding proteins homologous to PstS and PstC of Escherichia coli, respectively. Together with the previously isolated M. tuberculosis gene similar to the E. coli pstA, these are included in a cluster encoding a second putative phosphate transport system. We demonstrate that pstS-3 encodes the previously described Ag 88, a 40 kDa M. bovis BCG culture filtrate antigen (immunodominant in H-2b haplotype type mice). Finally, a signature motif identifying integral transmembrane proteins of prokaryotic phosphate binding-dependent permeases is proposed.
Braibant
M
M
Institut Pasteur, Department of Virology, Brussels, Belgium.
Lefèvre
P
P
de Wit
L
L
Ooms
J
J
Peirs
P
P
Huygen
K
K
Wattiez
R
R
Content
J
J
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D015151:Immunoblotting; D008969:Molecular Sequence Data; D005810:Multigene Family; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics*; D009876:Operon; D010455:Peptides / Q000737:chemistry; D028044:Phosphate-Binding Proteins; D017422:Sequence Analysis, DNA
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D010455:Peptides; D028044:Phosphate-Binding Proteins; C102011:PstC-2 protein, Mycobacterium tuberculosis; C102010:PstS-3 protein, Mycobacterium tuberculosis; D003488:Cyanogen Bromide