Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test.
A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS-PAGE and Western blotting. A total of 205 A. pleuropneumoniae, strains including all 12 serotype reference strains and 13 strains representing 8 common bacterial species associated with swine or related to A. pleuropneumoniae, were tested by mixing 25 microL of polystyrene reagent with the same volume of a dense suspension of bacterial cells grown for 18 h. All A. pleuropneumoniae strains had been previously serotyped using standard procedures. The polystyrene agglutination test was rapid (less than 3 min) and easy to perform. Overall a very good correlation (97.3%) with the standard techniques was found. The sensitized polystyrene particles were stable for at least 6 mo.
Dubreuil
J D
JD
Groupe de recherche sur les maladies infectieuses du porc, Faculty of Veterinary Medicine, Montreal University, Saint-Hyacinthe, Quebec, Canada.
Letellier
A
A
Stenbaek
E
E
Gottschalk
M
M
D000189:Actinobacillus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000276:immunology; D000372:Agglutination Tests / Q000379:methods / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D011137:Polystyrenes; D012703:Serotyping / Q000379:methods / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D011137:Polystyrenes