title: PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments.
authors.affiliation: National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.
authors.forename: D C
authors.initials: DC
authors.lastname: Tessier
authors.forename: D Y
authors.initials: DY
authors.lastname: Thomas
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion; D013698:Templates, Genetic; D014498:Uracil
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D014498:Uracil