Expression and localization of a baculovirus protein phosphatase.
We have characterized the expression of a baculoviral gene, ptp, and determined the location of its gene product, a protein tyrosine/serine phosphatase (BV-PTP), during virus infection. Using an antibody raised to a BV-PTP fusion to glutathione S-transferase, we found that ptp was expressed as a 19 kDa polypeptide at late times during virus infection. However, we also found that BV-PTP was present in the virions of both the budded and occluded forms so that a low level of BV-PTP is also present at the beginning of the infection process. Biochemical fractionation also showed that BV-PTP was primarily localized to the cytoplasm in transfected cells but that BV-PTP was present in both the cytoplasm and the nucleus of baculovirus-infected cells. Immunoelectron microscopy revealed that BV-PTP was associated with fibrillar structures which form in both the cytoplasm and the nucleus of baculovirus-infected cells.
Li
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Department of Genetics, University of Georgia, Athens 30602-2603, USA.
Miller
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LK
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000201:enzymology* / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D018411:Spodoptera; D015678:Viral Structural Proteins / Q000235:genetics; D014771:Virion / Q000201:enzymology
D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases