title: Comparative CD and thermodynamic studies between sheared A:G and Watson-Crick A:U(T) base pairs in RNA and DNA.
abstract: Thermodynamic parameters for duplex formation were determined from CD melting curves for r(GGACGAGUCC)2 and d(GGACGAGTCC)2, both of which have been demonstrated to form two consecutive 'sheared' A:G base pairs at the center by our previous studies. The parameters were determined also for r(GGACUAGUCC)2 and d(GGACTAGTCC)2, where the A:G mismatches are replaced by Watson-Crick A:U(T) base pairs. Thermodynamic properties for duplex formation are compared between the sheared and the Watson-Crick base pairs, and between RNA and DNA. Difference in the thermodynamic stability is analyzed and discussed in terms of enthalpy and entropy changes.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Yokohama National University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katahira
authors.forename: J
authors.initials: J
authors.lastname: Saeki
authors.forename: M
authors.initials: M
authors.lastname: Kanagawa
authors.forename: M
authors.initials: M
authors.lastname: Nagaoka
authors.forename: S
authors.initials: S
authors.lastname: Uesugi
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D019277:Entropy; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA