Changes in activity of spinal cells with muscular input in rats with referred muscular hyperalgesia from ureteral calculosis.
In rats with hyperalgesia of the obliquus externus muscle (OE) from artificial calculosis of the ipsilateral upper ureter, changes in cell activity were studied in the ipsilateral spinal cord (T11-T12) versus control animals. In cases of hyperalgesia of high degree, in the dorsal horn (0-900 microns) the following were found: significantly higher percentages of cells driven by OE stimulation (P < 0.03) and of spontaneously active cells with OE input (P < 0.02); significantly higher frequency of background discharge of cells with OE input (P < 0.002); among cells driven by OE stimulation, significantly higher percentages of neurons with exclusively deep input (P < 0.0006) and of neurons with low mechanical threshold of activation (P < 0.03). In the intermediate region of the cord (900-1600 microns), a significantly higher percentage was found of spontaneously active cells with OE input (P < 0.009) while in the ventral horn (1600-2300 microns) no changes were detected. The results indicate that referred muscle hyperalgesia of high degree is accompanied by a state of central sensitization probably triggered by the abnormal afferent input from the visceral focus.
Giamberardino
M A
MA
Pathophysiology of Pain Laboratory, G. D. Annunzio University of Chieti, Italy.
Dalal
A
A
Valente
R
R
Vecchiet
L
L
D000200:Action Potentials; D000344:Afferent Pathways / Q000503:physiopathology; D000818:Animals; D004558:Electric Stimulation; D005260:Female; D006930:Hyperalgesia / Q000209:etiology / Q000503:physiopathology*; D018482:Muscle, Skeletal / Q000294:innervation*; D009431:Neural Conduction; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000503:physiopathology*; D014514:Ureteral Calculi / Q000503:physiopathology*; D014828:Vocalization, Animal