The role of the O-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by Aeromonas hydrophila serogroup O:34.
We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to colonize in vivo the germfree chicken gut. We found a good colonization when the strains were grown at 20 degrees C but not when they were grown at 37 degrees C. We previously described that these strains were able to form the O-antigen lipopolysaccharide (LPS) when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen LPS (rfb mutants), and showed that they were unable to colonize the germfree chicken gut. All these results prompted us to conclude that the O-antigen LPS, in these strains, is a main factor for colonization in this animal model system.
Merino
S
S
Departamento de Microbiología, Universidad de Barcelona, Spain.
Rubires
X
X
Aguillar
A
A
Guillot
J F
JF
Tomás
J M
JM
D016980:Aeromonas hydrophila / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D002645:Chickens; D015169:Colony Count, Microbial; D004251:DNA Transposable Elements / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D006041:Goats; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D016254:Mutagenesis, Insertional; D019081:O Antigens / Q000276:immunology*; D010957:Plasmids; D011817:Rabbits; D013696:Temperature; D013997:Time Factors
D004251:DNA Transposable Elements; D019081:O Antigens