title: Differential effects of Th1 and Th2 derived cytokines on NGF synthesis by mouse astrocytes.
abstract: During the course of brain injury and inflammation there is an increased secretion of neurotrophic substances by astrocytes. We have examined the effect of the Th2-derived cytokine IL-10 and the Th1-derived cytokines Il-2 and IFN-gamma on the secretion of NGF by mouse astrocytes. IL-10 induced a dose-dependent increase in NGF secretion which was blocked by anti-IL-10 antibody. In contrast, the Th1-derived cytokines IFN-gamma and IL-2 did not induce NGF synthesis. Moreover, INF-gamma completely inhibited the increase in NGF secretion induced by IL-10 whereas it had no effect on the induction of NGF by TNF-alpha. These results indicate that IL-10 similarly to other Th2-derived cytokines may provide a neurotrophic support to injured neurons via the induction of NGF synthesis, whereas the Th1-derived cytokine IFN-gamma antagonizes this effect.
authors.affiliation: Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. chaya@brosh.cc.biu.ac.il
authors.forename: C
authors.initials: C
authors.lastname: Brodie
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007376:Interleukin-2; D009414:Nerve Growth Factors; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma