Site-directed mutagenesis using a uracil-containing phagemid template.
Hagemeier
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Department of Pediatrics, Humboldt University, Berlin, Germany.
D001426:Bacterial Proteins / Q000378:metabolism; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011755:Pyrophosphatases / Q000172:deficiency; D012641:Selection, Genetic; D013698:Templates, Genetic; D014498:Uracil
D001426:Bacterial Proteins; D017931:DNA Primers; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D014498:Uracil; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase