title: Alveolar epithelial lining fluid cellularity, protein and endothelin-1 in children with congenital heart disease.
abstract: This study applied bronchoalveolar lavage (BAL) to children with congenital heart disease (CHD) prior to elective cardiac catheterization (n = 48), to determine the influence of pulmonary blood flow and viral infection on the alveolar epithelial lining fluid (ELF) concentration of leucocytes, protein and endothelin-1 (ET-1). Lower respiratory tract (LRT) viral infection was defined as either a positive immunofluorescence for virus, or a virus cultured from the bronchoalveolar lavage fluid (BALF). Haemodynamic status was determined at cardiac catheterization. Normative data for BALF, but not ELF parameters, were obtained from 26 asymptomatic, noninfected normal children undergoing elective surgery. In the absence of LRT infection, the BALF macrophage, lymphocyte and neutrophil differential in CHD was not significantly different from the normal controls. In CHD, both increased pulmonary-to-systemic flow ratio (Q'p/Q's) and increased pulmonary artery-to-left ventricular pressure ratio PAP/LVP were associated with a decrease in ELF protein (rs = -0.59; p < 0.0001; and rs = -0.50; p < 0.0001 respectively). A respiratory virus was isolated from the BALF in 8 (17%) of CHD children. Virus isolation was associated with an increased ELF total protein (p < 0.05 vs no infection), a decreased alveolar macrophage differential count (p < 0.01), and an increased neutrophil differential count (p < 0.05). ET-1 was detected in the BALF of 83% of the noninfected CHD children compared to only 23% of the controls (p < 0.001). ELF ET-1 concentrations did not correlate with haemodynamic status in CHD, but were up to 100 times higher than paired plasma levels. We conclude that, in congenital heart disease, both lower respiratory tract viral infection and increased pulmonary blood flow and/or pulmonary vascular pressure influence the alveolar milieu. High alveolar epithelial lining fluid concentrations of endothelin-1 occur in congenital heart disease, but the stimulus for pulmonary endothelin-1 production is unclear.
authors.affiliation: Dept of Thoracic Medicine, Royal Children's Hospital, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Grigg
authors.forename: S
authors.initials: S
authors.lastname: Kleinert
authors.forename: R L
authors.initials: RL
authors.lastname: Woods
authors.forename: C J
authors.initials: CJ
authors.lastname: Thomas
authors.forename: P
authors.initials: P
authors.lastname: Vervaart
authors.forename: J L
authors.initials: JL
authors.lastname: Wilkinson
authors.forename: C F
authors.initials: CF
authors.lastname: Robertson
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000821:virology; D006328:Cardiac Catheterization; D016022:Case-Control Studies; D002452:Cell Count; D019332:Endothelin-1 / Q000032:analysis*; D006330:Heart Defects, Congenital / Q000150:complications / Q000503:physiopathology*; D006801:Humans; D007223:Infant; D007962:Leukocytes; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011652:Pulmonary Circulation / Q000502:physiology; D012141:Respiratory Tract Infections / Q000150:complications / Q000175:diagnosis; D014777:Virus Diseases / Q000150:complications / Q000175:diagnosis
chemical_list: D019332:Endothelin-1