An analysis of the normal ranges of lymphocyte subpopulations in children aged 5-13 years.
Peripheral blood lymphocytes subsets were examined in 233 healthy school children aged 4.9-13.7 years at the time of examination. Lymphocyte subsets were characterized according to the following cluster of differentiation (CD) numbers: CD2, CD4, CD8 and CD19 and quantified according to both their absolute number and as a percentage of total lymphocytes. For the purpose of analysis, the LMS (lambda, mu, sigma) method was utilized, with a 3%-97% confidence interval. Smoothing of the generated curves, was by multiple regression analysis, using the least squares method. The results of these analyses indicate distinct trends as a child ages, both in absolute numbers and in the percentage of each cell type.


CONCLUSIONS
We characterized lymphocyte subsets in children aged 4.8-13.7 years. These data should prove of considerable value to pediatricians dealing with patients with known or suspected immunological problems, and ought to be used in place of the commonly used, but inappropriate, adult lymphocyte subset ranges.
Robinson
M
M
Department of Immunology, Charing Cross and Westminster Medical School, London, UK.
O'Donohoe
J
J
Dadian
G
G
Wankowicz
A
A
Barltrop
D
D
Hobbs
J R
JR
D000293:Adolescent; D000367:Age Factors; D018941:Antigens, CD19 / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D016516:CD4-CD8 Ratio; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D006801:Humans; D018655:Lymphocyte Count; D012016:Reference Values; D016176:T-Lymphocyte Subsets
D018941:Antigens, CD19; D000945:Antigens, Differentiation, T-Lymphocyte