Direct application of Etest to gram-positive cocci from blood cultures: quick and reliable minimum inhibitory concentration data.
The Etest was applied directly to 100 Gram-positive bacterial strains from blood cultures to measure their minimum inhibitory concentration (MIC). Results showed 100% concordance of MIC data between a direct Etest method and the standard Etest method for Streptococcus pneumoniae, beta-hemolytic streptococci, and viridans group streptococci. In addition, direct Etest for Staphylococcus aureus, coagulase negative staphylococci, Enterococcus faecium, and Enterococcus faecalis showed 83 to 100% correlation with standard Etests. These data indicate that the Etest is useful to obtain MIC data on Gram-positive cocci (especially streptococci) directly from positive blood cultures. The advantages of a direct Etest are two fold: MIC results can be obtained 24 hours earlier than standard methods and a more representative population of the bacterial isolate is tested.
Hong
T
T
Department of Pathology, Bronx-Lebanon Hospital Center, New York, USA.
Ndamukong
J
J
Millett
W
W
Kish
A
A
Win
K K
KK
Choi
Y J
YJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001769:Blood / Q000382:microbiology*; D002439:Cefotaxime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D003470:Culture Media; D004917:Erythromycin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006095:Gram-Positive Cocci / Q000187:drug effects*; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010400:Penicillin G / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D013845:Thienamycins / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D003470:Culture Media; D005839:Gentamicins; D010406:Penicillins; D013845:Thienamycins; D004917:Erythromycin; D014640:Vancomycin; D015378:Imipenem; D002439:Cefotaxime; D010400:Penicillin G