The effects of low concentrations of antibiotics on epithelial damage caused by non-typable Haemophilus influenzae and bacterial morphology.
Sterile culture filtrates from non-typable Haemophilus influenzae (NTHi) grown in medium containing no antibiotics or 0.25 MIC of amoxycillin, ciprofloxacin or loracarbef were examined for their effect on the ciliary beat frequency (CBF) and structure of human respiratory epithelium. CBF slowing was significantly (P < 0.05) less with 0.25 MIC of all three antibiotics. The epithelium was significantly (P < 0.05) less disrupted with ciprofloxacin. The morphology of NTHi infecting human adenoid organ cultures after 24 h with or without 0.25 MIC of the same antibiotics was measured by scanning electron microscopy. Only ciprofloxacin caused a significant (P < 0.05) change in morphology.
Tsang
K W
KW
Department of Thoracic Medicine, Royal Brompton National Heart and Lung Institute, London, UK.
Rutman
A
A
Kanthakumar
K
K
Palmer
A
A
Roberts
D
D
Tillotson
G
G
Cole
P J
PJ
Wilson
R
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002923:Cilia / Q000502:physiology; D004848:Epithelium / Q000473:pathology; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000472:pathogenicity / Q000648:ultrastructure; D006801:Humans; D009297:Nasal Mucosa / Q000473:pathology*
D000900:Anti-Bacterial Agents