title: Acetyl-CoA hydrolase involved in acetate utilization in Saccharomyces cerevisiae.
abstract: Acetyl-CoA hydrolase, catalyzing the hydrolysis of acetyl-CoA, is presumably involved in regulating the intracellular acetyl-CoA or CoASH pools. The yeast enzyme is encoded by ACHl (acetyl-CoA hydrolase) and the expression of ACH1 is repressed by glucose (Lee, F.-J.S., Lin, L.-W. and Smith, J.A. (1990) J. Biol. Chem. 265, 7413-7418). In order to study the biological function of the acetyl-CoA hydrolase, a null mutation (achl-1) was created by gene replacement. The mutation, while not lethal, slows down acetate utilization. In comparison to wild-type, homozygote achl-l diploids, the onset of sporulation was delayed. When measuring the levels of ACH1 mRNA and acetyl-CoA hydrolase activity, we demonstrated that ACHl was highly expressed during sporulation process. These results indicated that acetyl-CoA hydrolase in yeast cells involved in acetate utilization and subsequently affected the sporulation process.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, USA. fangjen@ntumcl.mc.ntu.edu.tw
authors.forename: F J
authors.initials: FJ
authors.lastname: Lee
authors.forename: L W
authors.initials: LW
authors.lastname: Lin
authors.forename: J A
authors.initials: JA
authors.lastname: Smith
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000104:Acetyl-CoA Hydrolase / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal / Q000235:genetics; D006868:Hydrolysis; D009154:Mutation; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D013172:Spores, Fungal
chemical_list: D000085:Acetates; D012331:RNA, Fungal; D012333:RNA, Messenger; D000104:Acetyl-CoA Hydrolase