Inhibition of duck hepatitis B virus replication by 2',3'-dideoxy-3'-fluoroguanosine in vitro and in vivo.
The antiviral activity of 2',3'-dideoxy-3'-fluoroguanosine (FdG) or its triphosphate was evaluated in the duck hepatitis B virus (DHBV) system in vitro and in vivo. In primary DHBV-infected hepatocytes FdG results in a dose-dependent inhibition of viral replication with a nearly complete inhibition at a concentration of 1 microM. Also in vivo, FdG treatment of DHBV-infected ducklings reduces DHBV DNA replication by more than 90%. These data demonstrate that FdG is a strong inhibitor of DHBV replication in vitro and in vivo.
Hafkemeyer
P
P
Department of Medicine, University of Freiburg, Germany.
Keppler-Hafkemeyer
A
A
al Haya
M A
MA
von Janta-Lipinski
M
M
Matthes
E
E
Lehmann
C
C
Offensperger
W B
WB
Offensperger
S
S
Gerok
W
W
Blum
H E
HE
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015139:Blotting, Southern; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015224:Dideoxynucleosides / Q000494:pharmacology* / Q000627:therapeutic use; D004372:Ducks / Q000821:virology*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000821:virology; D017403:In Situ Hybridization; D008099:Liver / Q000166:cytology / Q000821:virology; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004279:DNA, Viral; D015224:Dideoxynucleosides; C055315:3'-fluoro-2',3'-dideoxyguanosine