Improved extraction procedure for the assay of isosorbide-5-mononitrate in serum.
A procedure is described for the extraction of isosorbide-5-mononitrate from serum with diethyl ether-n-butyl acetate (15:1, v/v). After extraction, the diethyl ether is evaporated at room temperature and the nitrate remains dissolved in the relatively non-volatile n-butyl acetate phase. Glassware must be used throughout as the recovery of isosorbide-5-mononitrate is not reproducible when plastic tubes or plastic pipettes are used. The extraction recovery of isosorbide-5-mononitrate from serum is 67 +/- 7%, measured relative to external standards.
Pennings
J M
JM
Toxicology Laboratory, University Hospital of Leiden, Netherlands.
de Haas
J M
JM
D002849:Chromatography, Gas; D006801:Humans; D007548:Isosorbide Dinitrate / Q000031:analogs & derivatives* / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D014665:Vasodilator Agents / Q000097:blood*
D014665:Vasodilator Agents; D007548:Isosorbide Dinitrate; C030397:isosorbide-5-mononitrate