Effect of endurance physical training on rat liver adenylyl cyclase system.
The adaptive response to endurance exercise of the catecholamine- and glucagon-sensitive adenylyl cyclase system was studied in rat liver plasma membranes. Endurance exercise enhanced adenylyl cyclase system activation by cellular agonists (glucagon, isoproterenol), by stimulators of the enzyme catalytic subunit (forskolin, Mn2+), and by Gs-protein activators (GppNHp, fluoride). In addition, endurance exercise increased the levels of G50, Gi alpha, and G beta subunits. These results show that the adenylyl cyclase system becomes sensitized in response to physical training.
Nieto
J L
JL
Departamento de Bioquímica y Biología Molecular I, Facultad de CC. Químicas, Universidad Complutense, Madrid, Spain.
Díaz-Laviada
I
I
Guillén
A
A
Haro
A
A
D000222:Adaptation, Physiological; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D004789:Enzyme Activation; D005459:Fluorides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D010805:Physical Conditioning, Animal; D010807:Physical Endurance; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; C047975:cyanopindolol; D005934:Glucagon; D010869:Pindolol; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D005459:Fluorides