title: Structural and antifungal properties of a pathogenesis-related protein from wheat kernel.
abstract: We have purified and characterized a protein from the water-soluble fraction of wheat kernel (Triticum aestivum cv. S. Pastore) consisting of a single polypeptide chain blocked at its N-terminus by a pyroglutamate residue; the complete amino acid sequence has been determined by automated sequence analysis performed on peptide fragments obtained by enzymatic hydrolyses of the protein. Homology studies have shown that this protein is very similar (97% sequence identity) to the previously characterized wheatwin1 as well as to other members of the pathogenesis-related (PR) proteins of class 4; in analogy with wheatwin1, we have termed this protein wheatwin2. Both wheatwin1 and wheatwin2 have specific antifugal activity toward the wide-host-range pathogen Botrytis cinerea and the wheat-specific pathogenic fungi of wheat Fusarium culmorum and Fusarium graminearum of groups 1 and 2. On the basis of their structural and functional properties, wheatwin1 and wheatwin2 can be classified as members of the PR4 protein family; this represents the first report concerning the presence of this kind of protein in wheat.
authors.affiliation: Dipartimento di Agrobiologia e Agrochimica, Universitá della Tuscia, Viterbo, Italy. Caruso@tusmx1.utovrm.it
authors.forename: C
authors.initials: C
authors.lastname: Caruso
authors.forename: C
authors.initials: C
authors.lastname: Caporale
authors.forename: G
authors.initials: G
authors.lastname: Chilosi
authors.forename: F
authors.initials: F
authors.lastname: Vacca
authors.forename: L
authors.initials: L
authors.lastname: Bertini
authors.forename: P
authors.initials: P
authors.lastname: Magro
authors.forename: E
authors.initials: E
authors.lastname: Poerio
authors.forename: V
authors.initials: V
authors.lastname: Buonocore
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification; D002457:Cell Extracts / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D008666:Metalloendopeptidases / Q000378:metabolism; D003904:Mitosporic Fungi / Q000187:drug effects; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010935:Plant Diseases; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011761:Pyrrolidonecarboxylic Acid / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D002457:Cell Extracts; D010446:Peptide Fragments; D010455:Peptides; D010940:Plant Proteins; C062880:Win-1 proteins, plant; C053376:pathogenesis-related proteins, plant; D008666:Metalloendopeptidases; C039830:peptidyl-Lys metalloendopeptidase; D011761:Pyrrolidonecarboxylic Acid