Changes in lymphokine-activated killer activity in peripheral blood lymphocytes from canine transmissible venereal sarcoma models.
Time course changes in anti-tumor activity induced in peripheral blood lymphocytes (PBL) with recombinant human interleukin-2 (rhIL-2) and phytohemagglutinin-P (PHA) were studied in dogs implanted with canine transmissible venereal sarcoma (CTVS) as a tumor-bearing model. The rhIL-2-dependent and PHA-dependent cultures allowed selective proliferation of lymphocytes expressing Thy-1 antigens. The lymphocytes acquired a prolonged anti-tumor activity against the CTVS cells, starting from 2 weeks after the culture, indicating generation of lymphokine-activated killer (LAK) cells. The LAK cells showed serial growth in rhIL-2-containing culture medium for at least a further 2 weeks without loss of the anti-tumor activity.
Mizuno
S
S
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Fujinaga
T
T
Kurosawa
T
T
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000502:physiology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000276:immunology* / Q000473:pathology; D012749:Sexually Transmitted Diseases / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D011994:Recombinant Proteins