Identification of 120 kD and 30 kD receptors for human coronavirus OC43 in cell membrane preparations from newborn mouse brain.
A biotinylated virus overlay was used to identify a 120kD virus-binding molecule in dissociated newborn mouse brain (nmb) cell suspensions after separation of the proteins by polyacrylamide gel electrophoresis, electroblotting, and blockage of non-specific binding sites. The virus-binding molecule was not detected in adult mouse brain cell suspensions. Mannose- and glucose-rich glycoproteins from nmb cell membranes were selected by ConA-Sepharose (Pharmacia) chromatography. A 30kD virus-binding molecule was eluted by 0.2 M alpha-methyl-D-mannoside. O-linked sialic acid, a receptor component, was identified in the eluate.
Collins
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AR
Department of Microbiology, State University of New York at Buffalo, USA.
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D017934:Coronavirus / Q000502:physiology*; D028962:Coronavirus OC43, Human; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011991:Receptors, Virus / Q000302:isolation & purification* / Q000378:metabolism
D008562:Membrane Glycoproteins; D011991:Receptors, Virus