The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons.
Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares a similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression.
Baxi
M K
MK
Centre for Veterinary Science, Cambridge University Veterinary School, UK.
Efstathiou
S
S
Lawrence
G
G
Whalley
J M
JM
Slater
J D
JD
Field
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000502:physiology*; D018259:Herpesvirus 1, Human / Q000235:genetics; D006736:Horses; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D015347:RNA Probes; D016372:RNA, Antisense / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D013047:Specific Pathogen-Free Organisms; D012668:Trigeminal Ganglion / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics; D017735:Virus Latency / Q000235:genetics*; D017201:Virus Shedding
D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C095533:gene 63 protein, Equine herpesvirus 1