Cosmids and transcribed sequences from chromosome 11q23.
To obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome 11 was prepared from microcell hybrid A9 (neo11) cells containing a normal human chromosome 11 tagged with pSV2neo at 11p11.2. Among 15 radiation hybrid clones isolated, RH(11)-9 which contains a q23 fragment in addition to the neo integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 11q23 and 6 cosmids to 11p11.2. Exon amplification proceeded with 23 of the 59 cosmids and 16 putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH(11)-9 cells and putative exons also facilitate the positional cloning of genes in the 11q23 region.
Katoh
M
M
Department of Molecular and Cell Genetics, School of Life Sciences, Tottori University, Japan.
Nakagawa
Y
Y
Yawata
T
T
Kumano
S
S
Kobayashi
E
E
Kurimasa
A
A
Kugoh
H
H
Oshimura
M
M
D000818:Animals; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D003360:Cosmids; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger