Geranylgeraniol causes a decrease in levels of calreticulin and tyrosine phosphorylation of a 36-kDa protein prior to the appearance of apoptotic features in HL-60 cells.
It was demonstrated recently that geranylgeraniol (GGO) has potent apoptosis-inducing activity in various lines of tumor cells, including HL-60 cells. In the present study, we found that GGO markedly inhibited the expression of a calcium-binding protein, calreticulin, prior to the induction of apoptosis in HL-60 cells. Furthermore, we also observed a significant decrease in the tyrosine phosphorylation of a 36-kDa protein that is a major tyrosine-phosphorylated protein in HL-60 cells. These findings suggest that decreases in levels of calreticulin and in the tyrosine phosphorylation of the 36-kDa protein might be associated with the induction of apoptosis by GGO in HL-60 cells.
Nakajo
S
S
Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Okamoto
M
M
Masuda
Y
Y
Sakai
I
I
Ohsawa
S
S
Nakaya
K
K
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D037282:Calreticulin; D004224:Diterpenes / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D018832:Molecular Chaperones / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D004224:Diterpenes; D018832:Molecular Chaperones; D010446:Peptide Fragments; D010750:Phosphoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D019000:Phosphotyrosine; C017338:geranylgeraniol