Efficacy of the chitosan posttreatment in calcification prevention of the glutaraldehyde-treated porcine aortic noncoronary cusp implanted in the right ventricular outflow tract in dogs.
Calcific tissue failure results in poor performance of the bioprosthetic heart valve. Chitosan post-treatment has been shown to be effective in calcification prevention of the glutaraldehyde-treated bovine pericardium when implanted subdermally in rats for 12 weeks. The present study investigated the effectiveness of the chitosan posttreatment in prevention of calcification of the glutaraldehyde-treated porcine aortic noncoronary cusp 5 months after implantation in the right ventricular outflow tract (RVOT) in mongrel dogs. Either 0.625% glutaraldehyde-treated (Group 1, n = 6) or glutaraldehyde-chitosan-treated (Group 2, n = 6) porcine aortic noncoronary cusp with the aortic wall was sewn to the RVOT. Gross histological observations showed moderate calcification of the glutaraldehyde-treated cusps, but no calcification was noticed in the glutaraldehyde-chitosan-treated grafts at 5 months. This was confirmed by results of quantitative analyses for calcium in half of each explanted cusp with aortic wall. The calcium content of the 0.625% glutaraldehyde-treated cusps (Ca, 40.6 +/- 24.9 mg/g dry wt) was significantly (p < 0.01) higher than that of glutaraldehyde-chitosan-treated cusps (Ca, 1.3 +/- 0.29 mg/g dry wt). These findings suggest that chitosan post-treatment is effective in complete calcium mitigation of the glutaraldehyde-treated porcine aortic noncoronary cusps implanted in the RVOT in dogs.
Kuribayashi
R
R
Department of Cardiovascular Surgery, Akita University School of Medicine, Japan.
Chanda
J
J
Abe
T
T
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000473:pathology; D001672:Biocompatible Materials; D002114:Calcinosis / Q000517:prevention & control*; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D004285:Dogs; D005976:Glutaral / Q000633:toxicity*; D006350:Heart Valve Prosthesis; D006490:Hemostatics / Q000494:pharmacology*; D008970:Molecular Weight; D013054:Spectrophotometry, Atomic; D013552:Swine
D001672:Biocompatible Materials; D006490:Hemostatics; D002686:Chitin; D048271:Chitosan; D005976:Glutaral