title: Mycoplasma orale infection affects K+ and Cl- currents in the HSG salivary gland cell line.
abstract: The relations between K+ channel and Cl- channel currents and mycoplasma infection status were studied longitudinally in HSG cells, a human submandibular gland cell line. The K+ channel currents were disrupted by the occurrence of mycoplasma infection: muscarinic activation of K+ channels and K+ channel expression as estimated by ionomycin- or hypotonically induced K+ current responses were all decreased. Similar decreases in ionomycin- and hypotonically induced responses were observed for Cl- channels, but only the latter decrease was statistically significant. Also, Cl- currents could be elicited more frequently than K+ currents (63% of cases versus 0%) in infected cells when tested by exposure to hypotonic media, indicating that mycoplasma infection affects K+ channels relatively more than Cl- channels. These changes occurred in the originally infected cells, were ameliorated when the infection was cleared with sparfloxacin, and recurred when the cells were reinfected. Such changes would be expected to result in hyposecretion of salivary fluid if they occurred in vivo.
authors.affiliation: Department of Oral Biology, School of Dentistry, University of Washington, Seattle 98195, USA.
authors.forename: K T
authors.initials: KT
authors.lastname: Izutsu
authors.forename: S
authors.initials: S
authors.lastname: Fatherazi
authors.forename: C M
authors.initials: CM
authors.lastname: Belton
authors.forename: D
authors.initials: D
authors.lastname: Oda
authors.forename: F D
authors.initials: FD
authors.lastname: Cartwright
authors.forename: G E
authors.initials: GE
authors.lastname: Kenny
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D018118:Chloride Channels / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D004553:Electric Conductivity; D024841:Fluoroquinolones; D006801:Humans; D007038:Hypotonic Solutions; D015759:Ionomycin / Q000494:pharmacology; D009175:Mycoplasma Infections / Q000503:physiopathology*; D015221:Potassium Channels / Q000502:physiology*; D015363:Quinolones / Q000494:pharmacology; D013363:Submandibular Gland / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D018118:Chloride Channels; D024841:Fluoroquinolones; D007038:Hypotonic Solutions; D015221:Potassium Channels; D015363:Quinolones; D015759:Ionomycin; C061363:sparfloxacin; D002118:Calcium