title: Characteristics of a rat cortical collecting duct cell line that maintains high transepithelial resistance.
abstract: This study describes the establishment of a rat kidney cortical collecting duct (CCD) clonal cell line (RCCD1 cells) that maintains high transepithelial resistance and specific hormonal sensitivities. Immortalized cells were obtained by infection of primary cultured CCD cells with the wild-type simian virus 40. Grown on Petri dishes, RCCD1 cells are organized as monolayers of cuboid cells separated by tight junctions and form domes. Grown on permeable filters, confluent RCCD1 cells exhibit high transepithelial resistance (Rt: 2390 +/- 140 omega. cm2), transepithelial potential difference (PD) of -10.5 +/- 1.2 mV lumen negative, an associated short-circuit current (Isc) of 4.3 +/- 0.5 microA/cm2, and generated significant Na+, K+, H+ and HCO3- gradients, reflecting Na+ and H+ reabsorption and K+ and HCO3- secretion. RCCD1 cells exhibit features of both principal (PC) and intercalated (IC) cells. Consistent with PC phenotype, about 50% of the cells were positively stained by a PC-specific agglutinin. In situ hybridization studies revealed the presence of alpha, beta and gamma subunit mRNAs of the amiloride-sensitive epithelial Na+ channel and alpha 1 and beta 1 subunits of Na(+)-K(+)-ATPase. Moreover, Na(+)-K(+)-ATPase was immunolocalized at the basolateral side of the cells. Arginine vasopressin (AVP) induced a significant increase in both cellular cAMP content and Isc. Amiloride decreased in a dose-dependent manner Isc from untreated and AVP-treated RCCD1 cells. In addition, a barium-sensitive K+ conductance was evidenced in the apical side of the cells. Consistent with IC phenotype, isoproterenol (ISO) provoked a large increase in cellular cAMP and stimulated Isc. The effect of ISO on Isc was blocked by 5 x 10(-3) M DPC, a chloride channel blocker. Finally, AVP plus ISO had additive effect on Isc. Taken together, these results provide evidence that the RCCD1 cell line has maintained many of the original properties of rat CCD from which they were derived.
authors.affiliation: INSERM U246, Institut Fédératif de Recherches Cellules Epithéliales, Faculté de Médecine Xavier Bichat, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Blot-Chabaud
authors.forename: M
authors.initials: M
authors.lastname: Laplace
authors.forename: F
authors.initials: F
authors.lastname: Cluzeaud
authors.forename: C
authors.initials: C
authors.lastname: Capurro
authors.forename: R
authors.initials: R
authors.lastname: Cassingéna
authors.forename: A
authors.initials: A
authors.lastname: Vandewalle
authors.forename: N
authors.initials: N
authors.lastname: Farman
authors.forename: J P
authors.initials: JP
authors.lastname: Bonvalet
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002460:Cell Line; D016764:Cell Polarity; D000242:Cyclic AMP / Q000378:metabolism; D017097:Electric Impedance; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D017403:In Situ Hybridization; D017136:Ion Transport; D007545:Isoproterenol / Q000494:pharmacology; D007672:Kidney Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D001127:Arginine Vasopressin; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D007545:Isoproterenol