The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer.
At the IVth and Vth Workshop on Human Leukocyte Differentiation Antigens a group of monoclonal antibodies recognizing myeloid cells was found to bind to the ganglioside X3-NeuAcVII3FucnLc10Cer (VIM-2 dodecasaccharide). These antibodies were given the provisional cluster of differentiation designation CDw65. Three antibodies of this cluster (VIM-2, VIM-8, and VIM-11) have now been studied in detail at the molecular and the cellular level. Binding of VIM-2 is abolished after treatment of cells with Vibrio cholerae neuraminidase, whereas VIM-8 and VIM-11 show enhanced binding to neuraminidase-treated cells. We investigated binding of the three mAbs to glycolipid antigens with shorter carbohydrate chains. Distinct differences were observed in the binding of CDw65 antibodies to VIII3-NeuAcV3FucnLc8Cer (VIM-2 decasaccharide). VIM-2 strongly bound to this antigen, whereas no binding was observed with the other two mAbs. Conversely, the asialoganglioside of the VIM-2 decasaccharide, V3FucnLc8Cer, was not recognized by VIM-2, but this antigen bound strongly VIM-8 and VIM-11. Thus, VIM-2 and the other CDw65 antibodies represented two different antigen specificities.
Kniep
B
B
Abteilung Zellbiologie und Immunologie, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, FRG.
Peter-Katalinic
J
J
Müthing
J
J
Majdic
O
O
Pickl
W F
WF
Knapp
W
W
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005732:Gangliosides / Q000378:metabolism; D006028:Glycosphingolipids / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology; D009439:Neuraminidase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C096764:CDw65 antigen, human; D005732:Gangliosides; D006028:Glycosphingolipids; C101242:fucosylneolactonorhexaosylceramide; C025447:sialogangliosides; D009439:Neuraminidase