Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering.
The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand, a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.
Jumel
K
K
Department of Applied Biochemistry and Food Science, University of Nottingham, UK.
Fiebrig
I
I
Harding
S E
SE
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005615:Freezing; D005753:Gastric Mucosa / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D007834:Lasers; D008027:Light; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry; D012542:Scattering, Radiation; D013552:Swine
D006023:Glycoproteins; D009077:Mucins