title: Vanadium catalysis in bromoperoxidation reaction.
abstract: Peroxidative bromination of phenol red to its tetrabromo derivative, bromophenol blue, required vanadate in addition to H202 when carried out in the pH range of 5-7. Excess H202, with ratio of H202:vanadate of 2:1 and above, prevented the reaction. Diperoxovanadate, known to be formed in such reaction mixtures, was ineffective by itself and needed uncomplexed vanadate (V(v)) or vanadyl (V(iv)) to support bromination. Bromide-assisted reduction of the excess vanadate to vanadyl appeared to be an essential secondary reaction. In the absence of phenol red oxygen was released, and concomitantly bromide was oxidized to a form competent to brominate phenol red added after termination of oxygen release. These findings indicated participation of reactions leading to an intermediate derived from vanadyl and diperoxovanadate, previously described from this laboratory (Arch. Biochem. Biophys. 316, 319-326, 1995). Continuous bromination of phenol red occurred when glucose oxidase-glucose system was used as a source of continuous flow of H202. A scheme of reactions involving peroxovanadates (mono-, di-, mu-, and bromo-) is proposed for the formation and utilization of an active brominating species and for the recycling of the product, mono-peroxovanadate, by H202, which explains the catalytic role of vanadium in the bromoperoxidation reaction.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore, India.
authors.forename: A V
authors.initials: AV
authors.lastname: Rao
authors.forename: H N
authors.initials: HN
authors.lastname: Ravishankar
authors.forename: T
authors.initials: T
authors.lastname: Ramasarma
mesh_terms: D001965:Bromides / Q000737:chemistry* / Q000378:metabolism*; D001978:Bromphenol Blue / Q000378:metabolism; D002384:Catalysis; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D010637:Phenolsulfonphthalein / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D014638:Vanadates / Q000378:metabolism; D014639:Vanadium / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001965:Bromides; D010710:Phosphates; D014639:Vanadium; D001978:Bromphenol Blue; D014638:Vanadates; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D010544:Peroxidases; C026195:bromide peroxidase; D010637:Phenolsulfonphthalein