title: Cloning single-stranded DNA.
abstract: DNA sequence and expression analyses have greatly benefited from using M13 and pUC derived cloning vectors and their polycloning sites. A chronology of the original concepts and experiments is reviewed.
authors.affiliation: Waksman Institute, State University of NJ, Piscataway 08855-0759, USA.
authors.forename: J
authors.initials: J
authors.lastname: Messing
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D017931:DNA Primers; D004277:DNA, Single-Stranded; D004279:DNA, Viral