Determination of thiocyanate anion by high-performance liquid chromatography with fluorimetric detection.
A simple and sensitive high-performance liquid chromatographic (HPLC) method was established for the trace determination of thiocyanate anion as a fluorogenic derivative. The method is based on the chemical derivatization of thiocyanate anion with 3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one. The resulting derivative was separated by a Nova-Pak C18 reversed-phase column. Optimization conditions for the derivatization of thiocyanate anion were investigated by HPLC with fluorimetric detection. The linear range for the quantitation of thiocyanate anion was 1-0.05 nmol in 0.1 mL of sample; the detection limit (with a signal-to-noise ratio of 5) of a 20-microL injected aliquot was approximately 3.3 +/- 1.2 fmol. Application of the method to the analysis of thiocyanate anion in saliva and plasma proved to be feasible.
Chen
S H
SH
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, Taiwan, Republic of China.
Yang
Z Y
ZY
Wu
H L
HL
Kou
H S
HS
Lin
S J
SJ
D000838:Anions / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry; D006801:Humans; D012463:Saliva / Q000737:chemistry; D013861:Thiocyanates / Q000032:analysis* / Q000097:blood; D013997:Time Factors
D000838:Anions; D013861:Thiocyanates