title: Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation.
abstract: Immunomagnetic separation (IMS) improved the detection of Coxiella burnetii in cow's milk by the polymerase chain reaction (PCR). IMS represents a novel approach to the isolation of microorganism from milk. The combination of IMS and PCR analysis has an adequate sensitivity for detection of C. burnetii.
authors.affiliation: Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: M
authors.initials: M
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Yanase
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.forename: C
authors.initials: C
authors.lastname: Morita
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003381:Coxiella / Q000302:isolation & purification*; D005260:Female; D018189:Immunomagnetic Separation / Q000662:veterinary; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012288:Rickettsiaceae Infections / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity