Sensitivity and specificity of anti-Jo-1 antibodies in autoimmune diseases with myositis.
OBJECTIVE
To determine the sensitivity and specificity of anti-Jo-1 in systemic sclerosis (SSc) patients with and without myositis.


METHODS
Immunoblots on HeLa nuclei were used to screen sera from 554 consecutive connective tissue disease patients. Those who had 45-55-kd bands, all patients with polymyositis/dermatomyositis (PM/DM), and a random selection of SSc, Raynaud's disease, systemic lupus erythematosus, and rheumatoid arthritis patients were also studied by anti-Jo-1 enzyme-linked immunosorbent assay and by immunoblots on rabbit pooled aminoacyl-transfer RNA synthetase.


RESULTS
Anti-Jo-1 was present only in 8 of the 40 PM/DM patients.


CONCLUSIONS
Anti-Jo-1 is specific for PM/DM.
Vázquez-Abad
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Division of Rheumatic Diseases, University of Connecticut School of Medicine, Farmington 06030-1310, USA.
Rothfield
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NF
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000150:complications / Q000276:immunology*; D003882:Dermatomyositis / Q000150:complications / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006643:Histidine-tRNA Ligase / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D017285:Polymyositis / Q000150:complications / Q000276:immunology*; D011817:Rabbits; D012595:Scleroderma, Systemic; D012680:Sensitivity and Specificity
D000906:Antibodies; D006643:Histidine-tRNA Ligase