Sequence analysis and distribution of two new human cathepsin L splice variants.
Despite elevated cathepsin L mRNA levels in kidney tumors, cathepsin L protein/activity was scarcely detectable in these tumors. As a possible reason, we detected two new splice variants of human cathepsin L mRNAs not identical to those previously reported. Besides the normal 'full-length' mRNA (hCATL-A) there is one form lacking 27 nucleotides (hCATL-A I) and another form lacking 90 nucleotides (hCATL-A II) in exon I. The splice variants do not influence the amino acid sequence of the translational product. hCATL-A and hCATL-A I probably form a secondary structure at the 5' non-coding sequence not present in hCATL-A II.
Rescheleit
D K
DK
Friedrich-Schiller-Universität Jena, Klinikum, Institut für Biochemie, Germany.
Rommerskirch
W J
WJ
Wiederanders
B
B
D017398:Alternative Splicing; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics*; D002460:Cell Line; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D012333:RNA, Messenger; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L