[Comparative study of chromatographic spectra of polypeptides extracted from pig spleen, liver, kidneys, thymus and periodontium].
Polypeptide extracts from the pig spleen, liver, kidney, thymus, pancreas and periodont were obtained by the acid extraction in presence of Zn and MG cations. This extracts were analyzed by HPLC with determining amino acid sequence using Edman's method. According to the analysis results several fragments of haemoglobin are present in the group of parenchymal organs (epsylon-chain pig haemoglobin 56-66 and 55-66, alpha-chain 48-61), the main mass of substances is localized in more hydrophilic part of chromatogram with 20 aminoacid N-terminal ubiquitin fragments; hydrophylic polypeptides ubiquitin and 2 hydrophobic polypeptides were also found in the group, but haemoglobin peaks were absent. The main mass of polypeptide substances in periodont extract is localized in the hydrophobic region, the peaks of haemoglobin and ubiquitin are absent. We suppose that each group of similar morpho-functional tissues has similar chromatographic picture of polypeptide extracts. It can be connected with the processing of protein structures specific for each organ or tissue. This characteristic for each tissue peptide can take part in the signal transduction at the level of single population cells or between physiologically correlated organs.
Kaĭdashev
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D000595:Amino Acid Sequence; D000818:Animals; D001704:Biopolymers; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000502:physiology; D010179:Pancreas / Q000737:chemistry; D010446:Peptide Fragments; D010455:Peptides / Q000302:isolation & purification*; D010519:Periodontium / Q000737:chemistry*; D013154:Spleen / Q000737:chemistry*; D013552:Swine; D013950:Thymus Gland / Q000737:chemistry*
D001704:Biopolymers; D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides