Co-expression of nociceptor properties in dorsal root ganglion neurons from the adult rat in vitro.
The cell body of sensory neurons in vitro has been used as a model to study the electrophysiological properties of afferent terminals. A limitation of this approach has been the ability to identify the function of the neuron studied. In the present study, we have tested the hypothesis that a putative nociceptor can be identified in vitro based on the expression of properties associated with nociceptors in vivo. A combination of patch-clamp electrophysiological and immunohistochemical techniques were used to describe the expression of nociceptor properties in acutely cultured dorsal root ganglion neurons from the adult rat. These properties include: a small cell body diameter; the presence of the neuropeptides substance P and calcitonin-gene related peptide; a shoulder (inflection) on the falling phase of the somal action potential, a response to the algogenic agent capsaicin, and sensitization in response to prostaglandin E2. Our results indicate that the frequency of expression of each of these properties varies in a manner consistent with that predicted from observations made in vivo, and that when one property is present in any given neuron, the other properties are also likely to be present. These data support the suggestion that the cell body of adult rat dorsal root ganglion neurons in vitro can be used to study the electrophysiological properties of nociceptors.
Gold
M S
MS
Department of Medicine, University of California, San Francisco 94143-0452, USA.
Dastmalchi
S
S
Levine
J D
JD
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000096:biosynthesis
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D015232:Dinoprostone; D002211:Capsaicin