title: Determination of hemoglobin adducts in workers exposed to 2,4, 6-trinitrotoluene.
abstract: 2,4,6-Trinitrotoluene (TNT) is an important occupational and environmental pollutant. TNT can be taken up through the skin and by inhalation. It is therefore essential to have fast and reliable methods to monitor human exposure. In rat experiments, it has been shown that TNT binds covalently to blood proteins and to tissue proteins. Hemoglobin (Hb) adducts of TNT are markers for the internal dose and possibly for the toxic effects of TNT, e.g. cataracts. In the present paper we introduce a new efficient method to quantify Hb adducts of TNT. Precipitated Hb was hydrolyzed with base in the presence of the surrogate internal standard 3,5-dinitroaniline (35DNA). The released 2-amino-4,6-dinitrotoluene (2ADNT) and 4-amino-2,6-dinitrotoluene (4ADNT) were quantified against 35DNA by gas chromatography-mass spectrometry with negative-ion chemical ionization. Hb of 50 workers and controls from a Chinese munition factory were investigated. The Hb adduct levels ranged from 3.7 to 522 ng for 4ADNT and from 0 to 14.7 ng for 2ADNT per gram of Hb. However, in control samples from Germany no Hb adducts of 4ADNT or 2ADNT could be found.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Würzburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Sabbioni
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: Y Y
authors.initials: YY
authors.lastname: Liu
mesh_terms: D004247:DNA / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000187:drug effects*; D006801:Humans; D016273:Occupational Exposure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014303:Trinitrotoluene / Q000633:toxicity*
chemical_list: D006454:Hemoglobins; D014303:Trinitrotoluene; D004247:DNA