Bacterial expression and immunological detection of human papillomavirus type 16 E7 protein.
We have expressed an unfused E7 protein from human papillomavirus 16 into Escherichia coli by using a T7-RNA polymerase system. E7 mRNA was detected one hour after promoter induction. Western blot analysis using either a murine monoclonal antibody elicited against E7 or sera from cervical carcinoma patients demonstrated that recombinant E7 expressed in E. coli reacted to both of them, and a 21 kD band is observed as a positive signal. This protein provides a suitable material for further protein structure and immunological studies and offers a screening tool for identification of circulating antibodies in human sera.
Moro
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Center for Genetic Engineering and Biotechnology, La Habana, Cuba. farma@ingen.cigb.edu.cu
Hernández
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D000595:Amino Acid Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000096:biosynthesis* / Q000276:immunology; D027383:Papillomaviridae / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D014158:Transcription, Genetic
D017931:DNA Primers; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C059731:oncogene protein E7, Human papillomavirus type 16