Principles and practice of peptide analysis with capillary zone electrophoresis.
Capillary electrophoresis provides a new analytical tool that complements reversed-phase high-performance liquid chromatography separations of peptides. The principles of this technique and their application to developing peptide analyses are described. Detailed recipes for useful buffers and for preparation of the instrument are provided. A sequence of generally useful experiments is suggested. Basic troubleshooting guidelines are provided.
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Thermo Capillary Electrophoresis, Franklin, MA 02038, USA.
D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides