A novel gene codes for a putative G protein-coupled receptor with an abundant expression in brain.
Following the cloning of the dopamine receptors we continued a search of the human genome for related genes. We searched an EST data base and discovered cDNA fragments encoding novel G protein-coupled receptor genes. The available GenBank sequence of one of these EST fragments showed that it encoded a receptor with closest similarity to the D2 dopamine and adrenergic receptors. This cDNA was used to isolate the gene (GPR19), and the encoded receptor also demonstrated similarity with the neuropeptide Y receptor. The gene was mapped to chromosome 12, in region p13.2-12.3. Northern blot analysis revealed expression of GPR19 in peripheral regions, and brain regions significantly overlapping with the D2 receptor gene expression. A sequence of the rat orthologue of GPR19 was obtained and in situ hybridization analysis demonstrated a very abundant expression in rat brain.
O'Dowd
B F
BF
Addiction Research Foundation and Department of Pharmacology, University of Toronto, Ont., Canada. bodowd@hookup.net
Nguyen
T
T
Lynch
K R
KR
Kolakowski
L F
LF
Thompson
M
M
Cheng
R
R
Marchese
A
A
Ng
G
G
Heng
H H
HH
George
S R
SR
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D018807:Polymorphism, Single-Stranded Conformational; D051381:Rats; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; C509756:GPR19 protein, human; C509757:GPR19 protein, rat; D009419:Nerve Tissue Proteins; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter; D019204:GTP-Binding Proteins