The affinity of allergen specific IgE and the competition between IgE and IgG for the allergen in Amb a V sensitive individuals.
We have calibrated a solid state RAST assay with affinity purified allergen-specific IgE. We then utilized the calibrated assay to measure the average affinity of individual IgE-containing sera in terms of the average association constant < K > for purified allergen Amb a V. The binding data yielded linear reciprocal plots indicating that the range of affinities of the responding clones was narrow. The range of the average association constant for the IgE-Amb a V complex was 0.9-26 x 10(10) M-1. The average affinity of the corresponding IgG response in the same individual, estimated by inhibition studies of IgE binding, was 10(7) M-1 in one case and lower than 10(6) M-1 in all the other cases.
Kim
K E
KE
Yonsei University, Seoul, Korea.
Rosenberg
A
A
Roberts
S
S
Blumenthal
M N
MN
D000485:Allergens / Q000276:immunology*; D000918:Antibody Specificity; D052179:Antigens, Plant; D001667:Binding, Competitive; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007700:Kinetics; D008433:Mathematics; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D012016:Reference Values
D000485:Allergens; D052179:Antigens, Plant; D007074:Immunoglobulin G; D010940:Plant Proteins; C069543:group 5 allergens, Ambrosia; D007073:Immunoglobulin E