Enzymatic synthesis of code regions for encoded combinatorial chemistry (ECC).
A synthesis method of oligonucleotides is proposed for generating the code regions for ECC (Encoded Combinatorial Chemistry). It exploits T4 RNA ligase with the use of ribonucleotide-containing-DNAs (RCDs). Preliminary success to ligate four fragments by this method is reported.
Kinoshita
Y
Y
Department of Functional Materials Science, Saitama University, Urawa, Japan.
Nishigaki
K
K
D001483:Base Sequence; D004253:DNA Nucleotidylexotransferase; D008722:Methods; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D011130:RNA Ligase (ATP); D012265:Ribonucleotides / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D012265:Ribonucleotides; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004253:DNA Nucleotidylexotransferase; D011130:RNA Ligase (ATP)