title: A recombinant antigen from the heartwater agent (Cowdria ruminatium) reactive with antibodies in some southeastern United States white-tailed deer (Odocoileus virginianus), but not cattle, sera.
abstract: Recombinant baculovirus techniques were used to express the 260 amino acid carboxyterminal portion of the 32 kilodalton (kDa) major antigenic protein (MAP 1) of Cowdria ruminantium, the heartwater agent, as a fusion protein. The recombinant MAP 1 was fused to an aminoterminal independently antigenic octapeptide sequence (FLAG peptide). Recombinant MAP 1 was used as an immunoblotting antigen to evaluate numerous reference antisera against organisms of the tribe Ehrlichieae. Monoclonal and polyclonal C. ruminantium antibodies, monoclonal anti-FLAG ascites, and antisera to Ehrlichia canis and Ehrlichia chaffeensis reacted with this antigen. Twelve of 79 sera collected 1980 to 1992 from southeastern U.S. white-tailed deer (Odocoileus virginianus) were also unexpectedly immunoblot-positive to MAP 1. These 12 deer sera had, as a group, significantly (P < 0.01) greater anti-E. chaffeensis titers (previously determined) than the sera from MAP 1 immunoblot-negative deer living in the same areas. None of the 262 sera from cattle living in the same areas were immunoblot-positive to MAP 1. All of an additional 50 cervine sera from Michigan (USA), 72 bovine sera from northern U.S. cattle, and 72 sera from Puerto Rican cattle were also immunoblot-negative to MAP 1. Sera from African sheep which were falsely seropositive to authentic MAP 1 were also immunoblot-positive to the recombinant MAP 1. Unidentified Ehrlichia spp. capable of serologic crossreactivity with the heartwater agent appear to be present in some southeastern U.S. white-tailed deer but not cattle. These or related Ehrlichia spp. may also be found elsewhere in the world in non-cervine species.
authors.affiliation: Diagnostic Virology Laboratory, United States Department of Agriculture, Ames, Iowa 50010, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Katz
authors.forename: A F
authors.initials: AF
authors.lastname: Barbet
authors.forename: S M
authors.initials: SM
authors.lastname: Mahan
authors.forename: D
authors.initials: D
authors.lastname: Kumbula
authors.forename: J M
authors.initials: JM
authors.lastname: Lockhart
authors.forename: M K
authors.initials: MK
authors.lastname: Keel
authors.forename: J E
authors.initials: JE
authors.lastname: Dawson
authors.forename: J G
authors.initials: JG
authors.lastname: Olson
authors.forename: S A
authors.initials: SA
authors.lastname: Ewing
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D003670:Deer; D016995:Ehrlichia ruminantium / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology; D015144:Southeastern United States
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D011994:Recombinant Proteins