title: Implementation of internal laboratory quality control procedures for the monitoring of ELISA performance at a regional veterinary laboratory.
abstract: Quality control (QC) procedures for antigen detection enzyme-linked immunosorbent assays (ELISAs) for hog cholera (HC) virus, foot and mouth disease (FMD) virus, and an antibody detection ELISA for FMD virus were established at a regional veterinary laboratory in northern Thailand. A recently developed computer software package, QCEL, was used to facilitate management and analysis of QC data. The program was used to assess test performance by producing Shewhart-CUSUM control charts which monitored control data for unacceptable fluctuations or trends. QCEL-generated control charts and analyses are presented and discussed. The use of a simple integrated computerised system for storage and analysis of QC control data provided the laboratory with the opportunity to achieve increased confidence in the results of tests performed.
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia. stuart@aahl.dah.csiro.au
authors.forename: S D
authors.initials: SD
authors.lastname: Blacksell
authors.forename: A R
authors.initials: AR
authors.lastname: Cameron
authors.forename: C
authors.initials: C
authors.lastname: Chamnanpood
authors.forename: P
authors.initials: P
authors.lastname: Chamnanpood
authors.forename: D
authors.initials: D
authors.lastname: Tatong
authors.forename: M
authors.initials: M
authors.lastname: Monpolsiri
authors.forename: H A
authors.initials: HA
authors.lastname: Westbury
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000032:analysis*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D006691:Classical Swine Fever / Q000276:immunology*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D007753:Laboratories / Q000592:standards*; D011786:Quality Control; D012984:Software; D013552:Swine; D014730:Veterinary Medicine
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral