Screening of the macromolecular component of CSF from various pathologies for its interference with GABAA receptor function.
Cerebrospinal fluid (CSF) samples from patients undergoing lumbar puncture for suspected neurological diseases were fractioned into macromolecular (MW > 3000 Daltons) and small molecule components (MW < 3000 Daltons). The macromolecular component was tested for its interference with GABA stimulation of 36Cl- accumulation in rat cerebral cortex microsacs. For many pathologies, no interference was apparent; an exception was two patients with Parkinson's disease in whom there were clear signs of stimulated GABA activity. This last result seems worthy of further investigation.
Rapallino
M V
MV
Centro di Neurofisiologia Cerebrale, CNR, Genova, Italy.
Cupello
A
A
Leonardi
A
A
D000328:Adult; D000368:Aged; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002713:Chlorine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid*; D011868:Radioisotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000134:cerebrospinal fluid
D011868:Radioisotopes; D011963:Receptors, GABA-A; D002713:Chlorine; D005680:gamma-Aminobutyric Acid