Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect.
To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug: repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.
den Hollander
J G
JG
Department of Clinical Microbiology and Antimicrobial Therapy, Erasmus University Rotterdam, Netherlands.
Mouton
J W
JW
Bakker-Woudenberg
I A
IA
Vleggaar
F P
FP
van Goor
M P
MP
Verbrugh
H A
HA
D000123:Acetyltransferases / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D005839:Gentamicins / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014031:Tobramycin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D014031:Tobramycin