Use of synthetic peptides to confirm that the Pseudomonas aeruginosa PAK pilus adhesin and the Candida albicans fimbrial adhesin possess a homologous receptor-binding domain.
Pseudomonas aeruginosa PAK pili and Candida albicans fimbriae are adhesins present on the microbial cell surfaces which mediate binding to epithelial cell-surface receptors. The receptor-binding domain (adhesintope) of the PAK pilus adhesin has been shown previously to reside in the carboxy-terminal disulphide-bonded region of P. aeruginosa pilin (PAK128-144). The delineation of the C. albicans fimbrial adhesintope was investigated in these studies using synthetic peptides which correspond to the whole (PAK128-144) or part of (PAK134-140) adhesintope of the PAK pilus and their respective anti-peptide antisera and biotinylated PAK pili (Bt-PAK pili), fimbriae (Bt-fimbriae), P. aeruginosa whole cells (Bt-P. aeruginosa) and C. albicans whole cells (Bt-C. albicans). The results from these studies confirmed that a structurally conserved motif akin to the PAK(128-144) peptide sequence is present in C. albicans fimbrial adhesin and that the seven-amino-acid residue PAK(134-140) sequence plays an important role in forming the adhesintope for both P. aeruginosa PAK pilus and C. albicans fimbrial adhesins.
Yu
L
L
Department of Medical Microbiology, University of Alberta, Edmonton, Canada.
Lee
K K
KK
Paranchych
W
W
Hodges
R S
RS
Irvin
R T
RT
D018829:Adhesins, Bacterial / Q000378:metabolism*; D000595:Amino Acid Sequence; D000906:Antibodies; D001665:Binding Sites; D002176:Candida albicans / Q000378:metabolism*; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D017124:Conserved Sequence; D004187:Disaccharides / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000378:metabolism; D006801:Humans; D037102:Lectins; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
C109455:ALA1 protein, Candida albicans; D018829:Adhesins, Bacterial; D000906:Antibodies; D015815:Cell Adhesion Molecules; D004187:Disaccharides; D005656:Fungal Proteins; D037102:Lectins; C038692:adhesin, Pseudomonas; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside