Peroxidation of brain lipids in vitro: nitric oxide versus hydroxyl radicals.
The pro-oxidant effects of hydroxyl radical (.OH, ferrous ammonium sulfate/Fe2+) or nitric oxide (NO., S-nitroso-N-acetylpenicillamine/SNAP) generating compounds were studied in rat brain homogenate preparations. Submicromolar concentrations of Fe2+, but not SNAP (up to 100 microM), increased the formation of fluorescent products of malondialdehyde in cortical homogenates. In fact, iron-catalyzed brain lipid peroxidation was inhibited by SNAP (100 microM), but not by light-exposed SNAP or its degradation product penicillamine (100 microM). This study provides relevant evidence to suggest that submicromolar concentrations of Fe2+ can potentiate lipid peroxidation in disrupted brain tissue. NO. released from SNAP did not stimulate, but rather inhibited brain lipid peroxidation. These results support the hypothesis that NO., as opposed to .OH radicals, is not a pro-oxidant but rather an antioxidant.
Rauhala
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P
Unit on Neurotoxicology and Neuroprotection, Laboratory of Clinical Science, National Institute of Mental Health, NIH Clinical Center, Bethesda, MD 20892-1264, USA.
Sziraki
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I
Chiueh
C C
CC
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005296:Ferrous Compounds / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism* / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D026423:S-Nitroso-N-Acetylpenicillamine
D005296:Ferrous Compounds; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D026423:S-Nitroso-N-Acetylpenicillamine; D010396:Penicillamine