Rearrangements involving repeated sequences within a P element preferentially occur between units close to the transposon extremities.
In a previous report we described rearrangements occurring at a high rate (30% of the progeny of dysgenic flies) within a cluster of 5S genes internal to a P element. These events were characterized as precise amplifications and deletions of 5S units. Here we analyze recombination events within P elements containing two repeated arrays of 5S genes flanking a central white gene. Deletions (50%) and duplications (3%) of the white gene together with various amounts of flanking 5S genes were observed. These recombinations occur preferentially between the most external 5S units of P transposons. Such rearrangements could be favored by interactions between the proteins bound to the P terminal sequences.
Pâques
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F
Laboratoire d'Embryologie Moléculaire et Expérimentale, Université Paris XI, Orsay, France.
Bucheton
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B
Wegnez
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M
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005127:Eye Color / Q000235:genetics*; D005136:Eye Proteins; D005260:Female; D005787:Gene Frequency; D015321:Gene Rearrangement; D007301:Insect Hormones / Q000235:genetics*; D008297:Male; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010641:Phenotype; D012091:Repetitive Sequences, Nucleic Acid; D014170:Transformation, Genetic
D018528:ATP-Binding Cassette Transporters; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D005136:Eye Proteins; D007301:Insect Hormones; C059254:w protein, Drosophila