Determination of glibenclamide in human plasma by solid-phase extraction and high-performance liquid chromatography.
A sensitive high-performance liquid chromatographic method for determination of intact glibenclamide in human plasma has been developed. Sample clean-up prior to chromatographic analysis was accomplished by extraction of the drug using a solid-phase RP-8 or RP-18 cartridge instead of the conventional liquid-liquid extraction methods described. For the separation of the drug from the endogenous components a reversed-phase column (LiChrosorb RP-8) of 5 microns particle size and 250 x 4 mm I.D., together with a mobile phase consisting of aceronitrile-12 mM perchloric acid (47:53) was selected. The method employs progesterone as an internal standard, and a reversed-phase column combined with UV detection of the drug at 230 nm. The detector response was linear up to the concentration of 400 ng/ml and the average recovery was 100.36%. The sensitivity of the method was 5 ng/ml.
Valdes Santurio
J R
JR
Dpto. de Estabilidad, Centro de Investigation y Desarrollo de los Medicamentos, MINSAP, Ciudad Habana, Cuba.
González Porto
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005905:Glyburide / Q000097:blood*; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity
D007004:Hypoglycemic Agents; D005905:Glyburide