Influence of dolichol on microsomal membrane functions.
Microsomal membranes from rat liver were extracted with n-pentane in order to remove the lipid products of the mevalonate pathway, dolichol, ubiquinone and cholesterol. Dolichol and cholesterol were subsequently reincorporated into these extracted membranes. Electron microscopic examination demonstrated that extraction did not alter the vesicular membrane structure of the microsomes. The extracted vesicles were permeable to uncharged molecules in the same manner as control microsomes but had an increased permeability for charged molecules. Enzyme denaturation was not observed. The contraction of extracted vesicles was greatly increased when the incubation medium was supplemented with non-penetrating compounds such as polyethylene glycol and was much greater than that of control microsomes. When extracted membranes were reconstituted with dolichol or cholesterol, the original lower degree of contraction was reestablished. The effects of dolichol reincorporation on a number of microsomal enzyme activities were investigated and some limited changes were observed. These results demonstrate that extraction of microsomes with n-pentane and subsequent reincorporation of dolichol is an effective approach for investigating the functions of this lipid. Dolichol and cholesterol both affect microsomal membrane fluidity, but only cholesterol modifies the activities of certain integral microsomal membrane enzymes to a larger extent.
Aberg
F
F
Department of Biochemistry, Stockholm University, Sweden.
Jakobsson-Borin
A
A
Olsson
M
M
Brunk
U
U
Dallner
G
G
D000818:Animals; D002784:Cholesterol / Q000302:isolation & purification / Q000378:metabolism; D004286:Dolichols / Q000302:isolation & purification / Q000378:metabolism*; D005612:Freeze Drying; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008027:Light; D008297:Male; D008560:Membrane Fluidity; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000378:metabolism* / Q000648:ultrastructure; D010316:Particle Size; D010420:Pentanes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation
D004286:Dolichols; D010420:Pentanes; C033353:pentane; D002784:Cholesterol