The PGK-PCR clonality assay (PPCA).
The PGK-PCR assay offers an accurate method for determination of clonal derivation of cells in females who are heterozygous for the PGK BstXI polymorphism. Techniques to screen for heterozygosity and determine clonality using polymerase chain reaction based techniques are provided.
Busque
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Haematology and Immunology Centre, Hôpital Maisonneuve-Rosemont, Canada.
Gilliland
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DG
D004247:DNA; D004303:Dosage Compensation, Genetic; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome
D004247:DNA; D010735:Phosphoglycerate Kinase