Analytical and clinical relationships between human IgG autoantibodies to beta 2 glycoprotein I and anticardiolipin antibodies.
OBJECTIVE
To examine IgG anti-beta 2 glycoprotein I (anti-beta 2 GPI) binding in 82 sera referred for anticardiolipin antibody (aCL) testing and to develop preliminary clinical correlations with antiphospholipid syndrome (APS).


METHODS
Immunoassay of IgG cofactor dependent aCL and IgG anti-beta 2 GPI antibodies and retrospective chart review.


RESULTS
Forty-four sera exhibited normal (< or = 22 GPL units) aCL activity, 18 had moderate binding activity (23-45 GPL units), and 20 had high (> or = 46 GPL units) binding activity to cardiolipin. Among these groups, 6 of the 20 sera in the high GPL group had elevated anti-beta 2 GPI. This correlated strongly with 2 or more clinical manifestations of APS.


CONCLUSIONS
Anti-beta 2 GPI activity may be a more valuable indicator of APS than aCL.
El-Kadi
H S
HS
Department of Medicine, Seton Hall University School of Graduate Medical Education, Paterson, New Jersey, USA.
Keil
L B
LB
DeBari
V A
VA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D017153:Antibodies, Anticardiolipin / Q000032:analysis*; D016736:Antiphospholipid Syndrome / Q000175:diagnosis / Q000276:immunology*; D001053:Apolipoproteins / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology*; D016009:Chi-Square Distribution; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011485:Protein Binding; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I
D017153:Antibodies, Anticardiolipin; D001053:Apolipoproteins; D006023:Glycoproteins; D007074:Immunoglobulin G; D053482:beta 2-Glycoprotein I