title: Sensitive high-performance thin-layer chromatography method for detection and determination of ranitidine in plasma samples.
abstract: A high-performance thin-layer chromatographic procedure has been developed for the determination of ranitidine, a H2-receptor antagonist, in plasma. The detection and quantification were performed without using internal standards. A single-stage extraction procedure was followed for extracting ranitidine from plasma, and a known amount of the extract was spotted on precoated silica gel F254 plates. Ranitidine was quantified using a Shimadzu CS930 dual-wavelength TLC scanner. The method provides a direct estimate of total ranitidine present in the plasma.
authors.affiliation: R&D Centre, Cadila Labs. Ltd, Ahmedabad, India.
authors.forename: V D
authors.initials: VD
authors.lastname: Mody
authors.forename: M C
authors.initials: MC
authors.lastname: Satia
authors.forename: T P
authors.initials: TP
authors.lastname: Gandhi
authors.forename: I A
authors.initials: IA
authors.lastname: Modi
authors.forename: R I
authors.initials: RI
authors.lastname: Modi
authors.forename: B K
authors.initials: BK
authors.lastname: Chakravarthy
mesh_terms: D000328:Adult; D001682:Biological Availability; D002138:Calibration; D002855:Chromatography, Thin Layer; D006207:Half-Life; D006635:Histamine H2 Antagonists / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011899:Ranitidine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards
chemical_list: D006635:Histamine H2 Antagonists; D007202:Indicators and Reagents; D011899:Ranitidine