Altered glycosylation of alpha(s)beta 1 integrins from rat colon carcinoma cells decreases their interaction with fibronectin.
Malignant cell transformation is generally accompanied by changes in their interactions with environing matrix proteins in a way to facilitate their migration and generate invasion. Our results show the binding of rat colon adenocarcinoma PROb cells to fibronectin strongly reduced when compared to normal rat intestine epithelial cells. This decrease was not due to the level of alpha(s)beta 1 integrins expressed at the surface of the cell line. However, beta 1- and alpha(s)-associated subunits appeared to be structurally altered as shown by immunoprecipitation followed by electrophoresis. Pulse chase experiments using 35S methionine evidenced differences in the biosynthesis of beta 1- and alpha(s)associated integrins: normal epithelial IEC18 cells required 16 h for maximal biosynthesis of the completely mature beta 1 subunit, while PROb cells did it within 4-6 h. Studies using endoglycosidases O, H, D, and N glycanase confirmed that the molecular weight alterations were due to abnormal glycosylation and suggested that alpha(s)beta 1 integrins of PROb cells could bear both mature complex and immature high mannose types while IEC18 cells borne only mature complex type oligosaccharidic chains. Treatment of both cell types with castanospermine, an inhibitor of N-glycosylation, reduced the differences observed in their adhesion to the fibronectin without significantly affecting beta 1 receptors expression at the cell surface. These results strongly suggest a role of the glycosylation of beta 1 receptors in the adhesion of rat colon adenocarcinoma PROb cells to fibronectin substrata.
Ringeard
S
S
Unité INSERM 419, Institut de Biologie, Nantes, France.
Harb
J
J
Gautier
F
F
Menanteau
J
J
Meflah
K
K
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006031:Glycosylation; D007212:Indolizines / Q000494:pharmacology; D019012:Integrin beta1 / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D014407:Tumor Cells, Cultured; D054836:Indolizidines
D005353:Fibronectins; D007212:Indolizines; D019012:Integrin beta1; D011956:Receptors, Cell Surface; C037806:castanospermine; D054836:Indolizidines