Rapid separation of gonadotropin-releasing hormone molecular forms by isocratic high-performance liquid chromatography on an ion-exchange column.
The purpose of the present work was to develop a chromatographic system for the separation of five molecular forms of the gonadotropin-releasing hormone (GnRH); mammalian GnRH (mGnRH) (LHRH), salmon GnRH (sGnRH), chicken I GnRH (cIGnRH), chicken II GnRH (cIIGnRH) and lamprey GnRH I (IGnRH-I). By using an ion-exchange HPLC column and isocratic elution, it was possible to separate properly the five peptides in approximately 20 min. The utility of the system in determining the GnRHs forms present in the brain of two species of vertebrates was examined.
D'Eramo
J L
JL
Laboratorio de Endocrinología Comparada, Fundación Centro de Investigaciones Médicas Albert Einstein, Buenos Aires, Argentina.
Somoza
G M
GM
Stefano
A
A
Canosa
L F
LF
Fridman
O
O
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
D007987:Gonadotropin-Releasing Hormone