Immunochemical methods for fumonisins.
Both monoclonal and polyclonal antibodies have been raised against fumonisins (Fm) and the hydrolyzed toxin in several laboratories. They have been used in several immunoassays and can detect 5 to 5000 ng of FmB1/mL in a "clean" standard solution. However, sample matrices still give severe interferences. Thus, without a cleanup treatment, most immunoassays can only be used as a screening tool for Fm in corn/feed in the 0.5 to 10 ppm range. The antibodies have also been used to generate anti-idiotype antibodies, to use as immunohistological reagents and to make affinity columns that are being used as a cleanup tool. In the present symposium, approaches used to generate antibodies, antibody specificity, sensitivity of various immunoassays and problems encountered in the immunoassays will be reviewed. Approaches used to overcome such problems and methods to improve the sensitivity and specificity of immunoassays of Fm are discussed.
Chu
F S
FS
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706, USA.
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D002274:Carcinogens, Environmental / Q000032:analysis*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins; D007118:Immunoassay / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1