Effect of TJ-960 (a Japanese herbal medicine) on free radical changes within an iron-induced focal epileptogenic region in rat brain measured by in vivo L-band electron spin resonance.
In vivo L-band electron spin resonance (ESR) system was used to measure the decay of nitroxide radicals in the living rat cerebrum containing a cortical area with an iron-induced epileptogenic region. Carbamoyl-PROXYL (3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolinyloxy) was injected intraperitoneally. The decay of nitroxide radicals in the iron-injected regions at the subacute stage of epileptogenesis was prolonged. The Japanese herbal medicine, sho-saiko-to-go-keishi-ka- shakuyakuto (TJ-960), a compound known to have free radical scavenging activity, inhibited the delay of nitroxide radical decay in the rat cerebrum with an iron-induced epileptic focus following injection of carbamoyl-PROXYL. TJ-960 did not affect the decay of nitroxide radicals in the control rats' brain. These data show that TJ-960 has the scavenging activity for free radicals generated within an iron-induced epileptogenic regions of rat brain.
Komatsu
M
M
Division of Medical Science, Institute for Life Support Technology, Yamagata Technopolis Foundation, Japan.
Hiramatsu
M
M
Yokoyama
H
H
Willmore
L J
LJ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003497:Cyclic N-Oxides; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D007501:Iron / Q000009:adverse effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D003497:Cyclic N-Oxides; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl; C018142:saiko-keishi-to; D007501:Iron