title: Laser induced resonance energy transfer--a novel approach towards achieving high sensitivity in capillary electrophoresis. I. Clinical diagnostic application.
abstract: Most of the procedures currently performed by capillary zone electrophoresis (CZE) with laser induced fluorescence detection requires prior derivatization. This increases cost, the turn-around-time and chances of extraneous contaminations. CZE with laser induced resonance energy transfer is demonstrated as a viable alternative for detecting non-fluorescent compounds with no prior derivatization. The feasibility of this approach is demonstrated by separating and directly detecting salicylic acid (2,4-dihydroxybenzoic acid), gentisic acid (o-methoxybenzoic acid), salicyluric acid (o-hydroxyhippuric acid) and 4-aminosalicylic acid in urine. The detection of salicylate in serum is also shown. The method is highly sensitive with detection limits in the 1.10(-7) M range. Importantly it requires no prior preconcentration or sample preparation and can be used with complex sample matrices such as serum and urine.
authors.affiliation: Department of Pathology, University of Texas-Medical Branch, Galveston 77555, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Petersen
authors.forename: M G
authors.initials: MG
authors.lastname: Bissell
authors.forename: A A
authors.initials: AA
authors.lastname: Mohammad
mesh_terms: D010131:Aminosalicylic Acid / Q000652:urine; D001241:Aspirin / Q000097:blood / Q000378:metabolism* / Q000652:urine*; D001565:Benzoates / Q000097:blood / Q000652:urine*; D002624:Chemistry, Clinical / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004735:Energy Transfer / Q000528:radiation effects*; D005841:Gentisates; D006626:Hippurates / Q000652:urine; D006801:Humans; D062385:Hydroxybenzoates / Q000652:urine; D007834:Lasers; D016014:Linear Models; D012459:Salicylates / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity
chemical_list: D001565:Benzoates; D005841:Gentisates; D006626:Hippurates; D062385:Hydroxybenzoates; D012459:Salicylates; C000693:salicylurate; D010131:Aminosalicylic Acid; D001241:Aspirin; C010925:2,5-dihydroxybenzoic acid