Isolation of two strains (S-R type) of Bacillus circulans and purification of a cyclomaltodextrin-glucanotransferase.
From wild type inoculum of Bacillus circulans DF 9 which produce cyclomaltodextrin-glucanotransferase (EC 2.4.1.19, CGTase), two different kinds of colonies were isolated, which correspond to the classical S-R variation. From the culture medium of both colonies grown together a CGTase was purified about 50 fold with a yield of 54% in two steps. From pure R-cell culture the enzyme was purified by about 38 folds with a yield of 79% in only one step, showing a complete homogeneity as judged by a native PAGE analysis.
Ferrarotti
S A
SA
Universidad Nacional de Luján, Departamento de Ciencias Básicas, Argentina.
Rosso
A M
AM
Maréchal
M A
MA
Krymkiewicz
N
N
Maréchal
L R
LR
D001407:Bacillus / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000302:isolation & purification*; D013045:Species Specificity
D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase