title: Kinamycin acetyltransferase I from Streptomyces murayamaensis, an apparently large, membrane-associated enzyme.
abstract: Identification and initial characterization of an apparently large, membrane-associated multifunctional enzyme, kinamycin acetyltransferase I (KAT I), is described. KAT I activity was enriched 29-fold over the level in cell-free extracts of Streptomyces murayamaensis. Two acetyltransferase activities catalyzing acetyl coenzyme A dependent conversion of kinamycin F and E to kinamycin E and D, respectively, were inseparable in the course of the partial purification. Partial purification involved separation of KAT I from cytosolic proteins by differential ultracentrifugation, solubilization with 0.5% CHAPS zwitterionic detergent followed by ultracentrifugation, and Sephacryl S400 gel filtration chromatography of the resulting supernatant.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis 97331-4003, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Gould
authors.forename: T
authors.initials: T
authors.lastname: O'Hare
authors.forename: P
authors.initials: P
authors.lastname: Seaton
authors.forename: J
authors.initials: J
authors.lastname: Soodsma
authors.forename: Z
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D002227:Carbazoles / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011809:Quinones / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D000095602:Angucyclines and Angucyclinones
chemical_list: D000123:Acetyltransferases; D002227:Carbazoles; D011809:Quinones; C059020:kinamycin E; D000095602:Angucyclines and Angucyclinones; C088442:kinamycin D; C059021:kinamycin F; C103393:kinamycin acetyltransferase I