High-performance separation methods in the analysis of a new peptide family: the galanins.
An analytical investigation of a new peptide family, the human galanins and their fragments, was carried out by reversed-phase HPLC, capillary zone electrophoresis (CZE) at different pH values and micellar electrokinetic capillary chromatography (MECC) in phosphate-borate-sodium dodecyl sulphate buffer. None of the methods seems to be superior to the others. The complementary nature of the electrophoretic methods is obvious when the profiles of peptides are compared; impurities not separated by HPLC are separated by CZE or MECC and vice versa. With these three different separation methods, a more complex analytical control of the synthetic work can be achieved.
Janáky
T
T
Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Szabó
E
E
Baláspiri
L
L
Adi
B
B
Penke
B
B
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D019004:Galanin / Q000138:chemical synthesis / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data
D019004:Galanin