Identification of thyrotropin-releasing hormone receptor mRNA in the Leydig cells of the mouse testis by in situ hybridization.
Thyrotropin-releasing hormone receptor (TRH-R) mRNA was detected in cryostat sections of the mouse testis using biotinylated oligonucleotides complementary to the cDNA encoding the mouse pituitary TRH-R by in situ hybridization. Hybridization signals were detected exclusively in the Leydig cells. The intensity of the signal was probe-concentration dependent. This result suggests that testicular TRH may serve as an autocrine regulator of reproductive function and development via TRH-R in a fashion that is similar or identical to that in the pituitary.
Zhang
S H
SH
Department of Anatomy, University of Hong Kong.
Zhang
Y Q
YQ
Vacca-Galloway
L L
LL
D000818:Animals; D001710:Biotin; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000737:chemistry*; D008297:Male; D051379:Mice; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis*; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D013973:Thyrotropin-Releasing Hormone; D001710:Biotin