A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus.
The cDNA corresponding to a fourth species of diacylglycerol (DG) kinase (EC 2.7.1.107) was isolated from cDNA libraries of rat retina and brain. This cDNA encoded a 929-aa, 104-kDa polypeptide termed DGK-IV. DGK-IV was different from previously identified mammalian DG kinase species, DGK-I, DGK-II, and DGK-III, in that it contained no EF-hand motifs but did contain four ankyrin-like repeats at the carboxyl terminus. These structural features of DGK-IV closely resemble the recently cloned, eye-specific DG kinase of Drosophila that is encoded by the retinal degeneration A (rdgA) gene. However, DGK-IV was expressed primarily in the thymus and brain with relatively low expression in the eye and intestine. Furthermore, the primary structure of the DGK-IV included a nuclear targeting motif, and immunocytochemical analysis revealed DGK-IV to localize in the nucleus of COS-7 cells transfected with the epitope-tagged cDNA, suggesting an involvement of DGK-IV in intranuclear processes.
Goto
K
K
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Kondo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology*; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D019852:Diacylglycerol Kinase; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012160:Retina / Q000378:metabolism
D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase