Detection of measles virus nucleoprotein mRNA in autopsied brain tissues.
By means of RT-PCR, a portion of measles virus (MV) mRNA encoding nucleoprotein (NP) could be detected in 11 (18%) of 61 brain tissue samples obtained from administrative autopsy cases, who apparently had not suffered from subacute sclerosing panencephalitis (SSPE)-like central nervous system disorders. Most of the brain-derived NP sequences showed significant asynonymous nucleotide substitutions when compared with wild-type MV isolates and SSPE virus. Our present results suggest that MV commonly persists in the human brain without causing apparent clinical symptoms, probably due to decreased virus replication.
Katayama
Y
Y
Department of Microbiology, Kobe University School of Medicine, Hyogo, Japan.
Hotta
H
H
Nishimura
A
A
Tatsuno
Y
Y
Homma
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D005260:Female; D005625:Frontal Lobe / Q000821:virology*; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D008457:Measles / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus