title: Fingerprinting of HLA class I genes for improved selection of unrelated bone marrow donors.
abstract: The degree of matching of HLA genes between the selected donor and recipient is an important aspect of the selection of unrelated donors for allogeneic bone marrow transplantation (UBMT). The most sensitive methods currently used are serological typing of HLA class I genes, mixed lymphocyte culture (MLC), IEF and molecular genotyping of HLA class II genes by direct sequencing of PCR products. Serological typing of class I antigenes (A, B and C) fails to detect minor differences demonstrated by direct sequencing of DNA polymorphic regions. Molecular genotyping of HLA class I genes by DNA analysis is costly and work-intensive. To improve compatibility between donor and recipient, we have set up a new rapid and non-radioisotopic application of the 'fingerprinting PCR' technique for the analysis of the polymorphic second exon of the HLA class I A, B and C genes. This technique is based on the formation of specific patterns (PCR fingerprints) of homoduplexes and heteroduplexes between heterologous amplified DNA sequences. After an electrophoretic run on non-denaturing polyacrylamide gel, different HLA class I types give allele-specific banding patterns. HLA class I matching is performed, after the gel has been soaked in ethidium bromide or silver-stained, by visual comparison of patients' fingerprints with those of donors. Identity can be confirmed by mixing donor and recipient DNAs in an amplification cross-match. To assess the technique, 10 normal samples, 22 related allogeneic bone marrow transplanted pairs and 10 unrelated HLA-A and HLA-B serologically matched patient-donor pairs were analysed for HLA class I polymorphic regions. In all the related pairs and in 1/10 unrelated pairs, matched donor-recipient patterns were identified. This new application of PCR fingerprinting may confirm the HLA class I serological selection of unrelated marrow donors.
authors.affiliation: Istituto di Ematologia L. e A. Seràgnoli, Università di Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Martinelli
authors.forename: P
authors.initials: P
authors.lastname: Farabegoli
authors.forename: M
authors.initials: M
authors.lastname: Buzzi
authors.forename: G
authors.initials: G
authors.lastname: Panzica
authors.forename: A
authors.initials: A
authors.lastname: Zaccaria
authors.forename: G
authors.initials: G
authors.lastname: Bandini
authors.forename: E
authors.initials: E
authors.lastname: Calori
authors.forename: N
authors.initials: N
authors.lastname: Testoni
authors.forename: G
authors.initials: G
authors.lastname: Rosti
authors.forename: R
authors.initials: R
authors.lastname: Conte
authors.forename: C
authors.initials: C
authors.lastname: Remiddi
authors.forename: M
authors.initials: M
authors.lastname: Salvucci
authors.forename: A
authors.initials: A
authors.lastname: De Vivo
authors.forename: S
authors.initials: S
authors.lastname: Tura
mesh_terms: D001483:Base Sequence; D001782:Blood Donors; D001853:Bone Marrow; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D006680:HLA Antigens / Q000145:classification* / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I