title: Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity.
abstract: The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013, causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP binding activity remained unaffected. A truncated MukB protein that is short of 109 amino acids from the C-terminus failed to bind DNA.
authors.affiliation: Department of Molecular Cell Biology, Kumamoto University School of Medicine, Japan.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Saleh
authors.forename: K
authors.initials: K
authors.lastname: Yamanaka
authors.forename: H
authors.initials: H
authors.lastname: Niki
authors.forename: T
authors.initials: T
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Yamazoe
authors.forename: S
authors.initials: S
authors.lastname: Hiraga
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation; D017384:Sequence Deletion; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C067035:MukB protein, E coli; D000255:Adenosine Triphosphate