Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide of Porphyromonas gingivalis.
Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced by P. gingivalis LPS was incompletely inhibited by polymyxin B. P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared with Escherichia coli LPS. Furthermore, P. gingivalis LPS, but not E. coli LPS, induced definite IL-6 production in C3H/HeJ mice. P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinated P. gingivalis LPS, similarly to E. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. Thus P. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells.
Shimauchi
H
H
Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, Japan.
Ogawa
T
T
Uchida
H
H
Yoshida
J
J
Ogoh
H
H
Nozaki
T
T
Okada
H
H
D000209:Acute-Phase Proteins; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins / Q000378:metabolism; D004926:Escherichia coli; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008562:Membrane Glycoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D010750:Phosphoproteins / Q000378:metabolism; D019000:Phosphotyrosine / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D019000:Phosphotyrosine; D011112:Polymyxin B; D000099231:Lipopolysaccharide-Binding Protein