title: Mitochondrial DNA restriction site map of Cochliomyia macellaria (Diptera:Calliphoridae).
abstract: The mitochondrial DNA of the secondary screwworm, Cochliomyia macellaria (F.), was cleaved with 15 restriction endonucleases to produce 54 restriction fragments. Forty-three restriction sites recognized by 12 enzymes were assigned positions on a restriction site map. Both the restriction fragments and the restriction site map were compared with published data from the screwworm, Cochliomyia hominivorax (Coquerel). The restriction site maps were aligned using a combination of highly conserved restriction sites. Estimated nucleotide divergence between the 2 species based on the fragments was 0.052. The divergence based on the restriction site map was 0.093. The data provide a baseline for future population or phylogenetic investigations.
authors.affiliation: Biosciences Research Laboratory, USDA-ARS, Fargo, ND 58105, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Roehrdanz
authors.forename: D A
authors.initials: DA
authors.lastname: Johnson
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000032:analysis*; D004175:Diptera / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004272:DNA, Mitochondrial