Disparity between tidal and static volumes of immature lungs treated with reconstituted surfactants.
We biologically assessed functions of several reconstituted surfactants with the same minimum surface tension (2-3 mN/m) as "complete" porcine pulmonary surfactant (natural surfactant) but with longer surface adsorption times. Administration of natural surfactant (adsorption time 0.29 s) into the lungs of surfactant-deficient immature rabbits brought a tidal volume of 16.1 +/- 4.4 (SD) ml/kg during mechanical ventilation with 40 breaths/min and 20 cmH2O insufflation pressure. In static pressure-volume recordings, these animals showed a lung volume of 62.4 +/- 9.7 ml/kg at 30 cmH2O airway pressure and maintained 55% of this volume when the pressure decreased to 5 cmH2O. With two reconstituted surfactants consisting of synthetic lipids or isolated lipids from porcine lungs plus surfactant-associated hydrophobic proteins (adsorption times 0.57 and 0.78 s, respectively), tidal volumes were < 38% of that with natural surfactant (P < 0.05), but static pressure-volume recordings were not different. Care is therefore needed in estimating the in vivo function of surfactant preparations from minimum surface tension or static pressure-volume measurements.
Kobayashi
T
T
Department of Anesthesiology and Intensive Care Medicine, School of Medicine, Kanazawa University, Japan.
Li
W Z
WZ
Tashiro
K
K
Takahashi
R
R
Waseda
Y
Y
Yamamoto
K
K
Suzuki
Y
Y
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D050356:Lipid Metabolism; D008168:Lung / Q000187:drug effects* / Q000254:growth & development; D008176:Lung Volume Measurements; D009929:Organ Size / Q000187:drug effects; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D013500:Surface Tension; D013552:Swine; D007507:Therapeutic Irrigation; D013990:Tidal Volume
D011663:Pulmonary Surfactants