MHV-3 induced prothrombinase is encoded by musfiblp.
Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. Musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
Parr
R L
RL
Department of Pathology and Laboratory Medicine, University of Texas Health Sciences Center, Houston, USA.
Fung
L
L
Reneker
J
J
Myers-Mason
N
N
Leibowitz
J L
JL
Levy
G
G
D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis / Q000096:biosynthesis; D018076:DNA, Complementary; D004790:Enzyme Induction; D005260:Female; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D013925:Thromboplastin / Q000096:biosynthesis*
D001779:Blood Coagulation Factors; D018076:DNA, Complementary; C045072:macrophage procoagulant activity; D013925:Thromboplastin