title: Increased macromolecular resonances in the rat cerebral cortex during severe energy failure as detected by 1H nuclear magnetic resonance spectroscopy.
abstract: Changes in cerebral macromolecular 1H nuclear magnetic resonance (NMR) spectrum were studied in cortical brain slices in vitro. Aglycaemic hypoxia irreversibly increased various short T2 spectral components at 1.8-0.8 ppm in concordance with energy loss and independent of T1 and T2 relaxation effects. Removal of external calcium (Ca2+e) slightly attenuated the effect. The results suggest NMR-visible reorganisation of intracellular proteins due to hypoxic insult, and show that it may be possible to monitor early cytoplasmic changes due to brain energy depletion by NMR spectroscopy.
authors.affiliation: A.I. Virtanen Institute, University of Kuopio, Finland.
authors.forename: J M
authors.initials: JM
authors.lastname: Hakumäki
authors.forename: O H
authors.initials: OH
authors.lastname: Gröhn
authors.forename: T R
authors.initials: TR
authors.lastname: Pirttilä
authors.forename: R A
authors.initials: RA
authors.lastname: Kauppinen
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004734:Energy Metabolism; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051381:Rats