title: Involvement of carnitine acyltransferases in peroxisomal fatty acid metabolism by the yeast Pichia guilliermondii.
abstract: This article provides information about peroxisomal fatty acid metabolism in the yeast Pichia guilliermondii. The existence of inducible mitochondrial carnitine palmitoyltransferase and peroxisomal carnitine octanoyl-transferase activities was demonstrated after culture of this yeast in a medium containing methyl oleate. The subcellular sites and induction patterns were studied. The inhibition of carnitine octanoyl- and palmitoyl-transferases by chlorpromazine to a large extent prevented the otherwise observed metabolism-dependent inactivation of thiolase by 2-bromofatty acids in vivo. We concluded that the metabolism of long- and medium-chain fatty acids in the peroxisome of this yeast involved carnitine intermediates.
authors.affiliation: Laboratoire de Biotechnologie, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Campus Universitaire Montmuzard, Dijon, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Pagot
authors.forename: J M
authors.initials: JM
authors.lastname: Belin
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000037:antagonists & inhibitors; D018533:Biomass; D002333:Carnitine Acyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D009829:Oleic Acids / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000378:metabolism; D013104:Spheroplasts
chemical_list: D004791:Enzyme Inhibitors; D005227:Fatty Acids; D009829:Oleic Acids; C005576:methyl oleate; D002333:Carnitine Acyltransferases; D000101:Acetyl-CoA C-Acetyltransferase; D002746:Chlorpromazine