Isolation and characterization of hemolytic genes from Actinobacillus actinomycetemcomitans.
Periodontopathic Actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. In the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic DNA libraries of A. actinomycetemcomitans ATCC 43718 on blood agar plates. DNA hybridization analysis indicates that there were two distinct hemolytic genes present. Sonicated extracts from both Escherichia coli clones possessed hemolytic activities on horse, sheep and human erythrocytes, but not those of rabbit. Rabbit antiserum to A. actinomycetemcomitans ATCC 43718 whole cells inhibited the hemolytic activities of these clones.
Kato
T
T
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Kimizuka
R
R
Okuda
K
K
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics* / Q000472:pathogenicity*; D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D006461:Hemolysis / Q000235:genetics*; D006736:Horses; D006801:Humans; D066298:In Vitro Techniques; D011817:Rabbits; D015183:Restriction Mapping; D012756:Sheep
D004269:DNA, Bacterial; D006460:Hemolysin Proteins