n-3 polyunsaturated fatty acid-induced changes in the molecular species composition of diradylglycerol in murine peritoneal macrophages remain stable during incubation ex vivo.
Three-to four-week-old C57BL/6 mice were maintained for four weeks on diets in which the 10% lipid component was the ethyl esters of either corn oil or n-3 polyunsaturated fatty acids (n-3 PUFA). Incubation of macrophages ex vivo for 14 h, a normal incubation time used for macrophage studies, in the absence of fetal calf serum, did not diminish the extent of incorporation of 20:5n-3 (eicosapentaenoic acid) and 22:6n-3 (docosahexaenoic acid) moieties into membrane phospholipids and into diradylglycerol (DG) relative to that after a very abbreviated incubation time. We conclude that studies examining the effects of dietary n-3 PUFA on DG formation and related physiological effects in macrophages can be performed after a normal ex vivo incubation of at least 14 h without experiencing a significant loss of incorporated n 3 PUFA.
Marignani
P A
PA
Department of Medicine, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada.
Sebaldt
R J
RJ
D000821:Animal Feed; D000818:Animals; D001798:Blood Proteins; D015331:Cohort Studies; D004075:Diglycerides / Q000096:biosynthesis*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D006358:Hot Temperature; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
D001798:Blood Proteins; D004075:Diglycerides; D015525:Fatty Acids, Omega-3; C035561:diarachidonyl diglyceride