Cycle sequencing through GC-rich regions.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a number of methods used to sequence through GC-rich sections of DNA, and why it is preferable to expose X-ray film to 32P at -70 degrees C. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
D000818:Animals; D003195:Computer Communication Networks; D006801:Humans; D017422:Sequence Analysis, DNA