title: Transposon mutagenesis in Desulfovibrio desulfuricans: development of a random mutagenesis tool from Tn7.
abstract: The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10(-4) to 10(-3) into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10(-6) per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10.
authors.affiliation: Biochemistry Department, University of Missouri-Columbia 65211, USA. Judy_D._Wall@muccmail.missouri.edu
authors.forename: J D
authors.initials: JD
authors.lastname: Wall
authors.forename: T
authors.initials: T
authors.lastname: Murnan
authors.forename: J
authors.initials: J
authors.lastname: Argyle
authors.forename: R S
authors.initials: RS
authors.lastname: English
authors.forename: B J
authors.initials: BJ
authors.lastname: Rapp-Giles
mesh_terms: D001483:Base Sequence; D003227:Conjugation, Genetic / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D003901:Desulfovibrio / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial