Suppressive effects of chlorophyllin on mutagen-induced umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) and tumor promoter-dependent ornithine decarboxylase induction in BALB/c 3T3 fibroblast cells.
The potentially protective role of chlorophyllin, the sodium and copper salt of chlorophyll a against the initiation and promotion stages in carcinogenesis was studied by in vitro short-term assays. Chlorophyllin showed a dose-dependent suppressive effect on 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol (Trp-P-1)-induced umu C gene expression of Salmonella typhimurium (TA 1535/pSK 1002) in the presence of metabolizing enzyme mixture. The similar inhibitory effect of chlorophyllin was detected in mitomycin C (MMC)-dependent umu C gene expression in the absence of metabolizing enzyme mixture. Furthermore chlorophyllin also exhibited a dose-dependent inhibition on 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity of 3T3 fibroblast cells at the same concentrations. However, when chlorophyll a isolated from Japanese tea leaves was applied on the same assay systems as a comparative experiment, chlorophyll a showed much weaker activity compared with that of chlorophyllin. The significance of this finding is discussed from the viewpoint of the protective role of chlorophyllin against carcinogenesis.
Okai
Y
Y
Division of Food and Nutrition, Osaka Kun-ei Women's College, Japan.
Higashi-Okai
K
K
Yano
Y
Y
Otani
S
S
D016475:3T3 Cells; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D002735:Chlorophyllides / Q000008:administration & dosage*; D004259:DNA-Directed DNA Polymerase; D004790:Enzyme Induction / Q000187:drug effects; D029968:Escherichia coli Proteins; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D065108:Ornithine Decarboxylase Inhibitors; D012486:Salmonella typhimurium / Q000235:genetics*
D016587:Antimutagenic Agents; D001426:Bacterial Proteins; D002735:Chlorophyllides; D029968:Escherichia coli Proteins; D065108:Ornithine Decarboxylase Inhibitors; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D009955:Ornithine Decarboxylase; C007020:chlorophyllin