Infection of the upper extremity by Mycobacterium marinum in a 3-year-old boy--diagnosis by 16S-rDNA analysis.
A 3-year-old boy developed several subcutaneous nodular lesions on his right arm. Based on the histological examination of one of these nodules furunculosis was suspected and cefuroxime was tentatively given. However, acid-fast bacilli were then detected in the tissue specimen and a few colonies of acid fast, gram-positive rods grew on blood agar. Definitive species diagnosis (Mycobacterium marinum) was rapidly achieved by automated sequencing of amplified 16S-rDNA and antimicrobial therapy was adjusted according to the available literature. After 3 weeks of treatment with clarithromycin, rifampicin and protionamid regression of the nodular lesions was evident.
Feddersen
A
A
Institut für Medizinische Mikrobiologie und Hygiene, Hochhaus am Augustusplatz, Mainz, Germany.
Kunkel
J
J
Jonas
D
D
Engel
V
V
Bhakdi
S
S
Husmann
M
M
D001132:Arm / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D006801:Humans; D008297:Male; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S