Covalent binding of carbon tetrachloride reactive metabolites to liver microsomal and nuclear lipid and phospholipid classes from Sprague Dawley and osborne Mendel male rats.
The interaction between carbon tetrachloride (CCl4)-reactive metabolites and liver microsomal or nuclear lipids (covalent binding = CB) was studied in male rats from Osborne Mendel (OM) and Sprague Dawley (SD), strains with different cancer susceptibility. The in vitro or in vivo CB was more intense in OM than in SD. Most of the CB was with phospholipid (PL; SD > OM). The CB to cholesterol (CH) and cholesterol esters (CHE) was OM > SD. We also observed the presence of specific adducts only present in lipids from either OM or SD strains. The results were related to the well-known role of PL and CH derivatives in gene function control and cell growth.
Fanelli
S L
SL
Centro de Investigaciones Toxicológicas (CEITOX) CITEFA/CONICET, Buenos Aires, Argentina.
Castro
J A
JA
D000818:Animals; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D010743:Phospholipids; D002251:Carbon Tetrachloride