Comparison of three power supplies used for the single-cell gel assay.
The alkaline single-cell gel (SCG) assay, which determines DNA damage in mice treated with 100 mg/kg methyl methanesulfonate (MMS), was run by using three power supplies from Bio-Rad (models 3000Xi, 200/2.0, and Power Pac 300). Comparisons of the results obtained from the use of these power supplies showed differences in mean DNA tail length to width ratios and profiles of these ratios in individual cells. Model 200/2.0 power supply appeared to enhance significantly the sensitivity of the assay. In purchasing power supplies, there is a need to evaluate their effect on the sensitivity of the test.
Vrzoc
M
M
Department of Biological Sciences, University of Windsor, Ontario, Canada.
Petras
M
M
D000818:Animals; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D005821:Genetic Techniques / Q000295:instrumentation*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results
D004247:DNA