title: Evaluation of an antigen detection-ELISA test for the diagnosis of trypanosomiasis in naturally infected cattle.
abstract: The sensitivity and the specificity of the antigen detection ELISA proposed by Nantulya and Lindqvist (1989) for the diagnosis of African Animal Trypanosomiasis have been assessed in naturally-occurring infections. 1633 cattle were sampled in trypanosomiasis endemic area and examined for trypanosomes by darkground/phase contrast buffy-coat method described by Murray et al. (1977) and for circulating antigen by ELISA. Fifty sera from Markoye, a tsetse free area in north of Burkina Faso, and 49 sera from Germany were also tested. In trypanosomiasis infested area, BCT detected 144 (8.8%) positive animals while Ag-ELISA revealed 65.8% of positive. Out of the 144 BCT-parasite-positive, Ag-ELISA enable to detect 75% of positive. The predominant trypanosomes identified by BCT was Trypanosoma vivax followed by T. congolense while Ag-ELISA indicated T. congolense followed by T. brucei. Ag-ELISA detected 76.5% out of the 51 T. congolense-BCT-positive and only 17% of all T. vivax BCT-positive. Cattle carring mixed infection involving two or three trypanosomes, particularly those with T. brucei and T. congolense are the most frequent. In tsetse free area, Ag-ELISA detected one positive cattle carring T. brucei and T. congolense and showed an apparent specificity of 98%. No serum from Germany was detected positive. This study suggests the joint use of Ag-ELISA and BCT for the diagnosis of trypanosomiasis particularly in epidemiological study in endemic area.
authors.affiliation: Centre International de Recherches-Dévéloppement sur l'Elevage en zone Sub-humide, Bobo-Dioulasso, Burkina Faso.
authors.forename: Z
authors.initials: Z
authors.lastname: Bengaly
authors.forename: A B
authors.initials: AB
authors.lastname: Kanwe
authors.forename: G
authors.initials: G
authors.lastname: Duvallet
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D051379:Mice; D012680:Sensitivity and Specificity; D014346:Trypanosoma brucei brucei / Q000276:immunology* / Q000302:isolation & purification; D014348:Trypanosoma congolense / Q000276:immunology* / Q000302:isolation & purification; D016689:Trypanosoma vivax / Q000276:immunology* / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000097:blood / Q000469:parasitology*; D014354:Trypanosomiasis, Bovine / Q000097:blood / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan