title: The diabetic state increases the activity but not the number of peritoneal macrophages in the GK rat promoting the tube formation of cultured endothelial cells in rat aorta.
abstract: The influence of the diabetic state on the number and activity of intraperitoneal macrophages was investigated in the GK rat. This rat is a model of non-insulin-dependent diabetes mellitus and was established from the normal Wistar rat. The cell number of a macrophages preparation in diabetic GK rats (10-15 weeks of age) did not differ from that in normal Wistar rats (9-10 weeks of age). The co-cultured macrophages in diabetic GK rats (9-12 weeks of age) significantly increased the tube formation of aortic endothelial cells (EC) compared with that in age-matched Wistar rats. The conditioned medium obtained from a culture of macrophages from GK rats also increased tube formation more than that from a culture of macrophages from Wistar rats. The effect of macrophages in the GK rat was not influenced by interferon-gamma (160 and 460 pM), but macrophages in normal rats were activated by these concentrations of interferon-gamma. In conclusion, the diabetic state increased the activity but not the number of macrophages in the GK rat, to stimulate tube formation of EC. The tube-forming effect of macrophages in the diabetic state may depend on released factors which differ from that of normal macrophages.
authors.affiliation: Department of Chemical Pharmacology, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: B
authors.initials: B
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Okabe
authors.forename: I
authors.initials: I
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000502:physiology*; D008297:Male; D051379:Mice; D009389:Neovascularization, Pathologic / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007371:Interferon-gamma