title: Immunolocalization of vinculin in the heart of the early developing rat embryo.
abstract: BACKGROUND
  The interaction of cells and extracellular matrix (ECM) components is important in the morphogenesis of the developing heart and is thought be mediated in part by adhesion plaques associated with vinculin, paxillin, talin, integrin, and other proteins. We investigated the patterns of expression of vinculin in the early embryonic rat heart to evaluate the role of vinculin in cardiac morphogenesis.
  
  
  METHODS
  Vinculin expression was studied immunohistochemically in developing Sprague-Dawley embryonic rat hearts between days 11.5 and 15.5.
  
  
  RESULTS
  Vinculin expression was transient and specific in the aorticopulmonary septum on day 13.5 and in the conal septum on day 14.5, when the respective septations were complete. Less vinculin immunoreactivity was detected in the atrioventricular cushion or ventricular septum, where obvious morphological alteration takes place.
  
  
  CONCLUSIONS
  Sites that were immunoreactive for vinculin in the present study are reportedly associated with the distribution of neural cell adhesion molecules (N-CAM) or of soluble tropoelastin. Thus vinculin appears to play a key role in aorticopulmonary septation, where neural crest cells are transformed into ectomesenchyme. Vinculin appears to be involved in elastogenesis and is contributed by ectomesenchyme derived from the neural crest cells.
authors.affiliation: Department of Pediatrics, Shiga University of Medical Science, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okagawa
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Shimada
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D006321:Heart / Q000196:embryology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D016596:Vinculin / Q000378:metabolism*
chemical_list: D016596:Vinculin