Preparation and specificity of antibodies to an anti-tumor beta-glucan, lentinan.
Antibodies against beta-glucan, lentinan from "Shiitake" (Lentinus edodes), were raised in the rabbit by subcutaneous immunization. Our antibodies did not recognize the other polysaccharides such as amylose, dextran, laminarin and galactan. It was proved that lentinan contents in mushroom could be measured by ELISA with the anti-lentinan antisera. Its contents were 3.5 mg/g fresh weight in Lentinus edodes. However, lentinan was not contained in Agaricus brazei, Agaricus bisporus and Ramaria bitrytis.
Mizono
M
M
Graduate School of Science and Technology, Kobe University, Japan.
Minato
K
K
Tsuchida
H
H
D000818:Animals; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000276:immunology*; D001487:Basidiomycota; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007912:Lentinan / Q000276:immunology*; D011817:Rabbits
D000970:Antineoplastic Agents; D007912:Lentinan