title: Cycle sequencing through GC-rich regions.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a number of methods used to sequence through GC-rich sections of DNA, and why it is preferable to expose X-ray film to 32P at -70 degrees C. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D000818:Animals; D003195:Computer Communication Networks; D006801:Humans; D017422:Sequence Analysis, DNA