Expression of truncated midkine in human colorectal cancers.
Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transcription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated from of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.
Miyashiro
I
I
Department of Oncogene Research, Osaka University, Japan.
Kaname
T
T
Nakayama
T
T
Nakamori
S
S
Yagyu
T
T
Monden
T
T
Kikkawa
N
N
Nishisho
I
I
Muramatsu
T
T
Monden
M
M
Akiyama
T
T
D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D016207:Cytokines; D015870:Gene Expression; D006801:Humans; D000078792:Midkine; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
D002352:Carrier Proteins; D016207:Cytokines; D012333:RNA, Messenger; D000078792:Midkine