title: High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor.
abstract: High levels of loss of distal markers on 17p13.3 in breast cancer suggested the presence within the region of at least one tumour-suppressor gene. Here we describe the derivation of two biallelic polymorphisms from the 17p telomeric yeast artificial chromosome (YAC) TYAC98. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR analysis demonstrated that the high level of allelic imbalance observed in breast tumours represented loss of constitutional heterozygosity (LOH) and that this LOH extended to the telomere. Lung carcinoma (but not Wilms' tumour)-derived DNA again revealed a high level of loss of subtelomeric 17p sequences. Telomeric microsatellite polymorphisms from other chromosome arms did not show such elevated loss in either tumour type. This suggested that the 17p loss observed did not reflect a general telomeric instability and provided further evidence for the presence of a breast cancer tumour-suppressor gene in the distal region of 17p13.3.
authors.affiliation: CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
authors.forename: G R
authors.initials: GR
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Stack
authors.forename: M
authors.initials: M
authors.lastname: Santibáñez-Koref
authors.forename: D S
authors.initials: DS
authors.lastname: Liscia
authors.forename: T
authors.initials: T
authors.lastname: Venesio
authors.forename: J C
authors.initials: JC
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Helms
authors.forename: H
authors.initials: H
authors.lastname: Donis-Keller
authors.forename: D C
authors.initials: DC
authors.lastname: Betticher
authors.forename: H J
authors.initials: HJ
authors.lastname: Altermatt
authors.forename: P R
authors.initials: PR
authors.lastname: Hoban
authors.forename: J
authors.initials: J
authors.lastname: Heighway
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D016615:Telomere