title: A new method for removal of the 2'-TBDMS group under acidic conditions in chemical synthesis of RNA.
abstract: A new and convenient method for removal of the TBDMS group as a protecting group for the 2' hydroxyl function in chemical synthesis of RNA under mild acidic conditions by use of diluted acetic acid solutions and 0.01 M hydrochloric acid (pH 2.0) was developed.
authors.affiliation: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawahara
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Sekine
mesh_terms: D019342:Acetic Acid; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D000644:Quaternary Ammonium Compounds; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D012313:RNA; C009405:tetrabutylammonium; D006152:Cyclic GMP; D019342:Acetic Acid