Monoamine oxidase released into plasma treated with the hepatotoxin allyl formate.
Monoamine oxidase (MAO) released from the rat liver into plasma and the activity levels of lipid peroxide (LPO) and superoxide dismutase (SOD) in liver tissue were investigated after pretreatment of rats with the perilobular hepatotoxin allyl formate (AF). When 3H-pargyline was given to AF-pretreated rats, the levels of 3H-pargyline labelled MAO in rat plasma were increased to 38% (p < 0.01 vs control), but the radioactivity was decreased to 35% (p < 0.05 vs control). The molecular weight of MAO subunits in plasma was similar to that of MAO subunits in liver (about 60,000) as determined by SDS electrophoresis. Liver tissue LPO levels in these rats were increased, whereas SOD activity was decreased. These results suggest that MAO in liver mitochondria was released into plasma as a consequence of membrane disorder.
Obata
T
T
Department of Pharmacology, Oita Medical University, Japan.
Yamanaka
Y
Y
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D005563:Formic Acid Esters / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D008970:Molecular Weight; D008995:Monoamine Oxidase / Q000097:blood* / Q000378:metabolism; D010293:Pargyline / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors*
D005563:Formic Acid Esters; D010293:Pargyline; C034499:allyl formate; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase