Determination of 1-O-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, platelet-activating factor and related phospholipids in biological samples by high-performance liquid chromatography--tandem mass spectrometry.
Combining normal-phase HPLC separation and tandem mass spectrometric detection, using an ion-spray HPLC-MS interface, a quantitative method for acyl-platelet activating factor (acyl-PAF), platelet-activating factor (PAF) and related phospholipids was developed. Mass spectra, positive ions, showed intense [M+H]+ ions; collision-induced dissociation of protonated molecular ions gave characteristic daughter ions corresponding to the polar head. Detection limits of 0.1-0.3 ng injected were obtained by multiple reaction monitoring. Samples of human endothelial cells treated with compounds modulating the levels of acyl-PAF and PAF have been analyzed by the present technique, proving that this approach is suitable for biochemical studies.
Savu
S R
SR
IBMP, Institut für Biomedizinische und Pharmazeutische Forschung, Nürnberg-Heroldsberg, Germany.
Silvestro
L
L
Sörgel
F
F
Montrucchio
G
G
Lupia
E
E
Camussi
G
G
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology; D006801:Humans; D016014:Linear Models; D008244:Lysophosphatidylcholines / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry; D010742:Phospholipid Ethers / Q000032:analysis / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D014471:Umbilical Veins
C069030:1-acyl-2-acetyl-sn-glycero-3-phosphocholine; D008244:Lysophosphatidylcholines; D010742:Phospholipid Ethers; D010743:Phospholipids; D010972:Platelet Activating Factor; C037913:CV 3988