Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex.
p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p40-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH-oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p40-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40-phox is expressed in megakaryocytic cells, but not in erythroid cells.
Zhan
S
S
Pediatric Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Vazquez
N
N
Zhan
S
S
Wientjes
F B
FB
Budarf
M L
ML
Schrock
E
E
Ried
T
T
Green
E D
ED
Chanock
S J
SJ
D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D019070:Cell Lineage; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005796:Genes; D006105:Granulomatous Disease, Chronic / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry*; D019255:NADPH Oxidases / Q000737:chemistry*; D009928:Organ Specificity; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D009097:Multienzyme Complexes; D010750:Phosphoproteins; D012333:RNA, Messenger; C085072:neutrophil cytosol factor 40K; D019255:NADPH Oxidases