The effect of activated alveolar macrophages on experimental lung emphysema development. I. Protease and antiprotease activities in the culture medium of alveolar macrophages.
Aim of the present study was to evaluate cathepsin D, base protease, antiplasmin, antitrypsin and antichymotrypsin activities and protein content in the 24h culture medium of the alveolar macrophages (AM) deriving from the rats treated BCG-vaccine and from rats with papain-induced emphysema. In the culture medium of cells isolated from the rats which were given BCG or papain and BCG+papain we observed an increase of base protease activity and a decrease of cathepsin D activity comparing with control group. Increased antitrypsin activity in BCG and BCG+papain-treated rats and decreased antitrypsin activity in papain-treated rats were observed. There were not significant differences in antiplasmin and antichymotrypsin activities between examined groups. The obtained results indicate that activated pulmonary macrophages are one of the sources of the protease-antiprotease intraalveolar imbalance. However, increased production of proteolytic enzymes may not be the only factor responsible for the progression of lung emphysema in BCG-treated rats.
Sulkowski
S
S
Department of Pathological Anatomy, Medical Academy of Białystok.
Skrzydlewska
E
E
Sulkowska
M
M
Worowski
K
K
Chyczewski
L
L
Dziecioł
J
J
Famulski
W
W
Kozielec
Z
Z
D000818:Animals; D001500:BCG Vaccine / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002402:Cathepsin D / Q000378:metabolism; D002478:Cells, Cultured; D002918:Chymotrypsin / Q000378:metabolism; D004646:Emphysema / Q000139:chemically induced / Q000201:enzymology* / Q000628:therapy; D010450:Endopeptidases / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000201:enzymology* / Q000473:pathology; D008297:Male; D010206:Papain; D011480:Protease Inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014357:Trypsin / Q000378:metabolism
D001500:BCG Vaccine; D011480:Protease Inhibitors; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D010206:Papain; D002402:Cathepsin D