title: The influence of CD4+ T cells, HIV disease stage and zidovudine on HIV isolation in Bahia, Brazil.
abstract: HIV-1 isolation was attempted on 72 individuals, including persons with known HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ability to detect HIV-1 from culture supernatant by p24 antigen capture assay was evaluated by segregating patients by absolute CD4+ cell counts, clinical stage of disease, p24 antigenemia and zidovudine use. The likelihood of a p24 positive HIV culture was highest among patients with CD4+ T-cell counts below 200/ul and patients with advanced clinical disease. Use of zidovudine did not affect the rate of HIV positivity in cultures.
authors.affiliation: Laboratório de Retrovírus, Hospital Universitáriò Prof. Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Brites
authors.forename: C
authors.initials: C
authors.lastname: Pedroso
authors.forename: N
authors.initials: N
authors.lastname: Silva
authors.forename: W D
authors.initials: WD
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Badaró
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use*; D001938:Brazil; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D018709:Statistics, Nonparametric; D014776:Virus Cultivation; D015215:Zidovudine / Q000627:therapeutic use*
chemical_list: D000998:Antiviral Agents; D016655:HIV Core Protein p24; D015215:Zidovudine