[The perspective on chromosomal painting (FISH)].
With the recent advances in molecular biology, many sophisticated technologies have been developed. The technique of fluorescence in situ hybridization (FISH) is one of them, and is being widely used not only in research fields of molecular biology but also for diagnosis in clinical laboratories. In this mini review, I outline the representative techniques of FISH and its application to laboratory medicine such as diagnosis of genitic and neoplastic diseases. Standard FISH consists of two according to DNA probes used, namely whole chromosome painting and centromere staining. Standard FISH is very useful for analysis of genetic abnormalities. Whole chromosomal painting covering the whole chromosome facilitates the rapid analysis of chromosome structural abnormalities, and centromere staining using alphoid DNA probes allows rapid analysis of chromosome numerical aberrations. Comparative genomic hybridization which has been recently developed is a very attractive method to detect amplification and deletion of chromosomes and genes in malignant tumors, and now is considered a powerful strategy for search of new genes implicated in the development and progression of neoplasm.
Sasaki
K
K
Department of Pathology, Iwate Medical University, Morioka.
D002877:Chromosomes, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence