Stability of equine infectious anemia virus in Aedes aegypti (Diptera: Culicidae), Stomoxys calcitrans (Diptera:Muscidae), and Tabanus fuscicostatus (Diptera:Tabanidae) stored at -70 degrees C.
Equine infectious anemia virus (EIAV) was injected intrathoracically into Aedes aegypti, Stomoxys calcitrans, and Tabanus fuscicostatus, and fed to Ae. aegypti in suspensions of either artificial blood of Eagle's Minimum Essential Medium. Insects were stored at -70 degrees C for up to 9 months before testing for the presence of EIAV. The viral tissue culture titers detected from stored insects were similar to those from insects tested at time 0.
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Department of Entomology, Louisiana Agricultural Experiment Station, LSU Agricultural Center, Baton Rouge 70803, USA.
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D000330:Aedes / Q000821:virology*; D000818:Animals; D004175:Diptera / Q000821:virology*; D005615:Freezing; D004860:Infectious Anemia Virus, Equine / Q000302:isolation & purification*; D009117:Muscidae / Q000821:virology*