Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization.
Cardiotrophin-1 (CT-1) is a new member of the interleukin-6 cytokine family that was identified from a mouse embryoid body cDNA library by expression cloning. Mouse CT-1 induces features of hypertrophy in neonatal rat cardiac myocytes and binds to and activates the leukaemia inhibitory factor/gp130 receptor complex. In this work we report the isolation and characterization of cDNA and genomic clones encoding human CT-1. These clones encode a 201 amino acid protein that is 80% identical to the mouse protein. Human CT-1 produced by transfection of the cDNA clones into mammalian cells induces the hypertrophy of neonatal rat cardiac myocytes. Human and mouse CT-1 bind to the leukaemia inhibitory factor receptor on both human and mouse cell lines indicating a lack of species specificity. No binding to the human oncostatin M specific receptor was detected. A 1.7 kb CT-1 mRNA is expressed in adult human heart, skeletal muscle, ovary, colon, prostate and testis and in fetal kidney and lung. The coding region of CT-1 is contained on three exons and is located on human chromosome 16p11.1-16p11.2.
Pennica
D
D
Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080, USA.
Swanson
T A
TA
Shaw
K J
KJ
Kuang
W J
WJ
Gray
C L
CL
Beatty
B G
BG
Wood
W I
WI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D016384:Consensus Sequence; D016207:Cytokines / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005091:Exons; D005260:Female; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018121:Receptors, Cytokine / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D016207:Cytokines; D018076:DNA, Complementary; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D011994:Recombinant Proteins; C092073:cardiotrophin 1