Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation.
Immunomagnetic separation (IMS) improved the detection of Coxiella burnetii in cow's milk by the polymerase chain reaction (PCR). IMS represents a novel approach to the isolation of microorganism from milk. The combination of IMS and PCR analysis has an adequate sensitivity for detection of C. burnetii.
Muramatsu
Y
Y
Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Maruyama
M
M
Yanase
T
T
Ueno
H
H
Morita
C
C
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003381:Coxiella / Q000302:isolation & purification*; D005260:Female; D018189:Immunomagnetic Separation / Q000662:veterinary; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012288:Rickettsiaceae Infections / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity