Immunohistochemical detection of hepatitis C virus related C100-3 and core antigens in formalin-fixed liver tissue.
HCV C100-3 non-structural and core proteins have been detected by immunohistochemical methods on paraffin-embedded tissue sections using monoclonal antibodies in 22 cases of chronic hepatitis C. C100-3 protein was detected in cytoplasm and nuclei of hepatocytes whereas core protein was only detected in nuclei. The specificity of the nuclear localization of HCV antigens was discussed in relation to cross-reactivity of the anti core antibody with host-derived GOR antigen.
Chamlian
A
A
Laboratoire de Pathologie Cellulaire et Métabolique du Foie, Faculté de Médecine, Marseille, France.
Benkoël
L
L
Sahel
J
J
Cherid
A
A
Brisse
J
J
Ikoli
J F
JF
Lambert
R
R
De Lamballerie
X
X
D000956:Antigens, Viral; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000276:immunology / Q000473:pathology / Q000821:virology*; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology / Q000821:virology*; D014758:Viral Core Proteins / Q000032:analysis*; D017361:Viral Nonstructural Proteins / Q000032:analysis*
D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D005404:Fixatives; D018936:Hepatitis C Antigens; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D005557:Formaldehyde