title: Methods and reagents. preparing ultra-competent Escherichia coli.
abstract: Methods and Reagents is a unique monthly column that highlights current discussions in the newsgroup blonet.molblo.methds-reagnts, available on the internet. This month's column discusses how to get the most out of your transformation procedure and how to prepare your own ultra-competent Escherichia coli cells. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D003195:Computer Communication Networks; D003470:Culture Media; D018274:Electroporation / Q000379:methods; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013696:Temperature; D014169:Transformation, Bacterial
chemical_list: D003470:Culture Media; D013438:Sulfhydryl Compounds