Polyamine analysis using N-hydroxysuccinimidyl-6-aminoquinoyl carbamate for pre-column derivatization.
N-Hydroxysuccinimidyl-6-aminoquinoyl carbamate (AccQ.Fluor) was used as a polyamine pre-column derivatization reagent prior to HPLC analysis using a 5-micron C8 reversed-phase column. The fluorescence detector excitation wavelength was set at 250 nm and emission at 395 nm. Quantitation, reproducibility, linearity, recovery and stability were demonstrated. The lower limit of detection was 660 fmol. This method is 45 and 61 times more sensitive than those using the pre-column derivatizing agents dansyl chloride and orthophthalaldehyde, respectively. Applicability to biological samples was demonstrated by analyses of polyamines in extracts of mouse erythrocytes and Trypanosoma brucei brucei.
Merali
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S
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10016, USA.
Clarkson
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AB
D000634:Aminoquinolines / Q000737:chemistry*; D000818:Animals; D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry; D051379:Mice; D011073:Polyamines / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014346:Trypanosoma brucei brucei / Q000737:chemistry
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D011073:Polyamines