title: Potentiometric alternating biosensing system for in continuum toxicity tests on cell population.
abstract: In this paper, we investigate the possibility to monitor, in continuum, changes of cellular metabolism. Examples of this feature are shown: metabolic changes due to drugs administration are evaluated as a decrease of extracellular acidification rate on CHO-K1 and HeLa cells. Parallel experiments with a standard test were performed to validate the results obtained with the biosensor.
authors.affiliation: Technobiochip, Società di Ricerca Industriale Avanzata, Marciana (LI), Italy.
authors.forename: M
authors.initials: M
authors.lastname: Adami
authors.forename: I
authors.initials: I
authors.lastname: Zolfino
authors.forename: S
authors.initials: S
authors.lastname: Fenu
authors.forename: D
authors.initials: D
authors.lastname: Nardelli
authors.forename: C
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D015374:Biosensing Techniques; D016466:CHO Cells / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D006224:Cricetinae; D004594:Electrophysiology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008727:Methotrexate / Q000494:pharmacology; D011199:Potentiometry / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C001943:5-fluorouridine; D002945:Cisplatin; D014529:Uridine; D008727:Methotrexate