Changes in the organelle arrangement in primary cultured hepatocytes following the formation of cytoskeleton.
The relationship between the arrangement of organelles and cytoskeleton in primary cultured hepatocytes was studied morphologically by using fluorescent dyes. Double staining with DiOC6(3) and rhodamine-conjugated phalloidin revealed that the distribution of endoplasmic reticulum was identical with the distribution of F-actin in the marginal areas of well-spread hepatocytes. As the cells spread further, the mitochondria which were positively stained with rhodamine 123 began to show a radial distribution, extending from the centre of the cell towards its margins. This pattern was disturbed after the microtubules were depolymerized with colchicine. Simultaneous double staining using rhodamine-conjugated phalloidin and anti-tubulin antibody revealed that the distribution of stress fibres resembles that of microtubules in hepatocytes. These results suggest that the arrangement of organelles in hepatocytes is determined by the cytoskeleton.
Sawamoto
K
K
Division of Agricultural Chemistry, School of Agriculture, Meiji University, Kanagawa, Japan.
Takahashi
N
N
D000199:Actins / Q000378:metabolism; D000818:Animals; D001704:Biopolymers; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008870:Microtubules / Q000187:drug effects; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure*; D051381:Rats
D000199:Actins; D001704:Biopolymers; D005456:Fluorescent Dyes