In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours.
Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-PDT. Localisation of PpIX in the mitochondria was demonstrated by fluorescence microscopy. Subcellular damage following ALA-PDT was observed using transmission electron microscopy. This damage was localised initially to the mitochondria, with damage to membranes and the nucleus and complete loss of intracytoplasmic organisation being observed subsequently. There was no apparent difference in ALA-PDT response between a multidrug-resistant ovarian carcinoma cell line and its parent line. These results indicate that ALA-PDT has potential for application to therapy of gynaecological malignancies.
Rossi
F M
FM
Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.
Campbell
D L
DL
Pottier
R H
RH
Kennedy
J C
JC
Dickson
E F
EF
D000622:Aminolevulinic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D004351:Drug Resistance; D005260:Female; D005833:Genital Neoplasms, Female / Q000188:drug therapy* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D010778:Photochemotherapy; D011524:Protoporphyrins / Q000378:metabolism
D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX