Detailed characterization of antisense DNA oligonucleotides.
We have developed methods for verification of the structures of novel, chemically synthesized oligonucleotides having alternating methylphosphonate/phosphodiester internucleotide linkages. Matrix-assisted laser desorption ionization mass spectrometry was used to measure the molecular masses of full-length oligonucleotides, failure synthesis products, and degradation products formed by enzymatic and chemical means. These measurements provide detailed structural information, including molecular mass, length, base composition, complete nucleotide sequence, and confirmation of the sugar moieties and internucleotide linkages.
Keough
T
T
Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45253-8707, USA.
Shaffer
J D
JD
Lacey
M P
MP
Riley
T A
TA
Marvin
W B
WB
Scurria
M A
MA
Hasselfield
J A
JA
Hesselberth
E P
EP
D000818:Animals; D001482:Base Composition; D002241:Carbohydrates / Q000737:chemistry; D002417:Cattle; D016373:DNA, Antisense / Q000737:chemistry*; D007887:Fabaceae / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010946:Plants, Medicinal; D017422:Sequence Analysis, DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013154:Spleen / Q000201:enzymology
D002241:Carbohydrates; D016373:DNA, Antisense; D009708:Nucleotidases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010727:Phosphoric Diester Hydrolases