Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.
Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.
Versalovic
J
J
Division of Digestive Diseases, Baylor College of Medicine, Houston, Texas, USA. jamesv@bcm.tmc.edu
Shortridge
D
D
Kibler
K
K
Griffy
M V
MV
Beyer
J
J
Flamm
R K
RK
Tanaka
S K
SK
Graham
D Y
DY
Go
M F
MF
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D017291:Clarithromycin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012338:RNA, Ribosomal, 23S / Q000187:drug effects* / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000900:Anti-Bacterial Agents; D011500:Protein Synthesis Inhibitors; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin