A novel strategy for O6-protection of guanosine.
A "one-pot reaction" has been used for simultaneous protection of amino and O6-function of deoxyguanosine. N-(4-bromobutyl) phthalimide used for O6-protection generates an alkylamino function after mild base hydrolysis. Two sequences d(GGA) and d(GGATCC) have been synthesised with all protected guanosine units. Similarly, 2-(vinyl)pyridine has also been used for O6-protection following the same strategy.
Kumar
P
P
Department of Chemistry, University of Allahabad, India.
Dubey
K K
KK
Singh
R K
RK
Misra
K
K
D001483:Base Sequence; D003849:Deoxyguanosine / Q000138:chemical synthesis / Q000737:chemistry; D006151:Guanosine / Q000138:chemical synthesis* / Q000737:chemistry*; D007202:Indicators and Reagents; D008722:Methods; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010797:Phthalimides; D011725:Pyridines
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010797:Phthalimides; D011725:Pyridines; D006151:Guanosine; C030953:2-vinylpyridine; D003849:Deoxyguanosine