title: Detection and determination of the major metabolites of [3H]cytosine arabinoside by high-performance liquid chromatography.
abstract: An ion-pair high-performance liquid chromatographic assay involving solid-phase scintillation detection was established for the rapid identification and determination of all major metabolites of tritium-labelled cytosine arabinoside (Ara-C) in an in vitro system. In a single run of 50 min, Ara-C, Ara-CMP, Ara-CDP-choline, Ara-CDP, Ara-U, Ara-UMP, Ara-CTP, Ara-UDP and Ara-UTP can be measured. The method is fast, sensitive, with limits of detection ranging from 40 to 200 pg (absolute), and highly reproducible.
authors.affiliation: Department of Hematology and Oncology, University of Göttingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Braess
authors.forename: J
authors.initials: J
authors.lastname: Pförtner
authors.forename: C C
authors.initials: CC
authors.lastname: Kaufmann
authors.forename: B
authors.initials: B
authors.lastname: Ramsauer
authors.forename: M
authors.initials: M
authors.lastname: Unterhalt
authors.forename: W
authors.initials: W
authors.lastname: Hiddemann
authors.forename: E
authors.initials: E
authors.lastname: Schleyer
mesh_terms: D002138:Calibration; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003561:Cytarabine / Q000032:analysis* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003561:Cytarabine