Intracellular distribution of radio-labelled enterotoxigenic Escherichia coli heat-stable toxin (STa) in the intestine of suckling rat.
The chloramine T-procedure was employed to radio-iodinate Escherichia coli heat-stable toxin (STa). The percentage iodination yield was 53.14% with a specific activity of 25.9 microCi/micrograms. Intragastric injections of both labelled and unlabelled toxin administered to suckling rats produced significant increase of fluid accumulation indicating that the biological activity of STa was not lost during radio-iodination. The uptake of the labelled toxin by subcellular particles of the intestinal cells in the suckling rats indicated that the toxin uptake was maximum in mitochondrial-lysosomal fraction. This confirms our previous observation on lysosomal involvement in digestion of the toxin. Results of the study also seem to be consistent with the short-lived hyper-secretory action of the toxin.
Aye-Kyaw
Department of Medical Research, Yangon, Myanmar.
Moe-Khine-Pyi-Soe
Tin-Oo
Myo-Khin
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D010450:Endopeptidases / Q000378:metabolism; D004768:Enterotoxins / Q000032:analysis / Q000378:metabolism*; D004926:Escherichia coli; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000378:metabolism; D007457:Iodine Radioisotopes; D051381:Rats; D017208:Rats, Wistar
D004768:Enterotoxins; D007457:Iodine Radioisotopes; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D010450:Endopeptidases