title: PRL-1, a protein tyrosine phosphatase, is expressed in neurons and oligodendrocytes in the brain and induced in the cerebral cortex following transient forebrain ischemia.
abstract: Protein tyrosine phosphorylation is thought to play an important role in the regulation of neural function. We reported previously that CL100, a cytoplasmic type protein tyrosine phosphatase (PTP), was induced after transient forebrain ischemia. In the present study, changes in the mRNA levels after ischemia of PRL-1, a cytoplasmic type PTP and immediate-early gene similar to CL100, was examined. In situ hybridization histochemistry showed that PRL-1 mRNA was expressed in normal adult rats in neurons and oligodendrocytes in widespread regions including the cerebral cortex, hippocampus and cerebellum. PRL-1 mRNA was expressed in the developing brains on embryonic days 15 and 19 and postnatal day 1. Northern blot analysis showed that PRL-1 mRNA was induced from 6 h to 9 h after reperfusion in the cerebral cortex of postischemic rats. These findings suggest that PRL-1 plays a role in neurons and oliogodendrocytes, and that expression of PRL-1 mRNA is regulated by a mechanism different from those of other immediate-early genes such as c-fos and c-jun.
authors.affiliation: Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takano
authors.forename: H
authors.initials: H
authors.lastname: Fukuyama
authors.forename: M
authors.initials: M
authors.lastname: Fukumoto
authors.forename: J
authors.initials: J
authors.lastname: Kimura
authors.forename: J H
authors.initials: JH
authors.lastname: Xue
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: J
authors.initials: J
authors.lastname: Fujita
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D004790:Enzyme Induction; D017781:Genes, Immediate-Early; D017874:Immediate-Early Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D009928:Organ Specificity; D016548:Prosencephalon; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014158:Transcription, Genetic
chemical_list: D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D017027:Protein Tyrosine Phosphatases; C490199:Ptp4a1 protein, rat