Characterization of arachidonic acid metabolites through the 12-lipoxygenase pathway in human epidermis by high-performance liquid chromatography and gas chromatography/mass spectrometry.
Fragments of human epidermis were incubated in the presence of exogenous arachidonic acid. The incubates were subjected to reversed-phase and straight-phase high-performance liquid chromatography and finally the identity of the arachidonic acid metabolites of the 12-lipoxygenase pathway was determined by means of gas chromatography/mass spectrometry. It was found that human epidermis produces 8-hydroxy-11,12-epoxy- and 10-hydroxy-11,12-epoxyeicosatrienoic acid, 8,9,12-, 8,11,12- and 10,11,12-trihydroxyeicosatrienoic acid, 12-keto-eicosatetraenoic acid and 12-hydroxy-eicosatetraenoic acid derived from arachidonic acid via the 12-lipoxygenase pathway. As far as is known, this is the first time that a systematic and complete characterization of arachidonic acid metabolism through the 12-lipoxygenase pathway in human epidermis has been reported. Some of the eicosanoids characterized have potent biological activities that suggest their role in the inflammatory response on human skin.
Antón
R
R
Inflammation Mediators Laboratory, Institute of Research of Santa Creu i Sant Pau Hospital, Barcelona, Spain.
Abián
J
J
Vila
L
L
D000328:Adult; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001095:Arachidonic Acids / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004817:Epidermis / Q000737:chemistry* / Q000201:enzymology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D001095:Arachidonic Acids; D007202:Indicators and Reagents; D001092:Arachidonate 12-Lipoxygenase