Spaceflight effects on beta-adrenoceptor and metabolic properties in rat plantaris.
Effects of 14 days of spaceflight on beta-adrenoceptor (beta-AR), mitochondrial enzyme activities, and fiber type composition were studied in plantaris muscles of male adult Sprague-Dawley rats. The beta-AR was analyzed in cross sections by quantitative autoradiography. The maximum binding capacity (Bmax) of beta-AR was significantly lowered (approximately 29%) after flight, but the recovery was not completed within 9 days in 1-G environment. Because the dissociation constant remained unchanged, it is suggested that the changes in the Bmax were caused by the alteration of receptor number. The activities of succinate dehydrogenase (SDH) measured in whole homogenates were subnormal (approximately -24%) in muscles sampled approximately 5 h after flight but they were normalized during 9 days of recovery. The percent composition of fiber types and beta-hydroxyacyl CoA dehydrogenase (HAD) activity did not change significantly due to spaceflight. It is suggested that the spaceflight-induced decrease of the Bmax of beta-AR in plantaris was accompanied by a lowered activity of a mitochondrial inner-membrane enzyme SDH but not a matrix enzyme HAD.
Ohira
Y
Y
Department of Physiology and Biomechanics, National Institute of Fitness and Sports, Kanoya City, Japan.
Yasui
W
W
Kariya
F
F
Tanaka
T
T
Kitajima
I
I
Maruyama
I
I
Nagaoka
S
S
Sekiguchi
C
C
Hinds
W E
WE
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001345:Autoradiography; D001835:Body Weight / Q000502:physiology; D006651:Histocytochemistry; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009929:Organ Size / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013026:Space Flight; D013385:Succinate Dehydrogenase / Q000378:metabolism
D007457:Iodine Radioisotopes; D011943:Receptors, Adrenergic, beta; D015094:3-Hydroxyacyl CoA Dehydrogenases; D013385:Succinate Dehydrogenase