The effect of activated alveolar macrophages on experimental lung emphysema development. II. The study of fibroblast and alveolar macrophage co-culture.
The cell-cell interaction between fibroblasts and alveolar macrophages was examined using a co-culture system. Alveolar macrophages (AM) were harvested from the bronchoalveolar lavages (BAL) of rats with papain induced lung emphysema. The BCG-vaccine was applied as a macrophage mobilizing and activating agent. The morphological examinations carried out in scanning electron microscope (SEM) as well as the evaluation of the uptake of 3H-thymidine did not show any significant differences between respective co-cultures of fibroblasts and AM isolated both from the lungs of control and experimental animals (treated with BCG or papain, and BCG+papain). However, significant growth were noted in 3H-thymidine uptake between fibroblast cultures done with or without cells isolated from the lungs. The results obtained suggest that AM can promote fibroblast proliferation during the progression of experimental lung emphysema.
Sulkowska
M
M
Department of Pathological Anatomy, Medical Academy of Białystok.
Wołczyński
S
S
Sulkowski
S
S
Sobaniec-Lotowska
M
M
Chyczewski
L
L
Sulik
M
M
Kulikowski
M
M
Dziecioł
J
J
Berger
W
W
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018920:Coculture Techniques; D004646:Emphysema / Q000139:chemically induced / Q000503:physiopathology*; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure; D016676:Macrophages, Alveolar / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D010206:Papain; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine / Q000493:pharmacokinetics
D010206:Papain; D013936:Thymidine