title: DNA ploidy on cytologic and tissue sections of breast lumps. A comparison using image analysis.
abstract: OBJECTIVE
  Ploidy correlation between cytologic and histologic breast specimens would permit preoperative measurement of ploidy and may assist in patient management.
  
  
  METHODS
  DNA indices were measured on fine needle aspirates (FNAs) of 50 breast lumps and the results compared with ploidy of histologic specimens from the same lump.
  
  
  RESULTS
  Thirty-six of 50 were histologically malignant, and 14/50 were benign. The mean DNA index of malignant tissue sections was 1.23 (range, 0.61-2.62) and on corresponding cytology was 1.49 (range, 0.79-3.18). Fourteen benign tissue sections had a mean DNA index of 0.94 (range, 0.51-1.37) and a mean DNA index of 1.06 (range, 0.74-2.13) on cytology. DNA indices of FNAs and corresponding tissue sections revealed a correlation between the two (Pearson's correlation coefficient = .386, P value = .006).
  
  
  CONCLUSIONS
  Our results show that the DNA indices are comparable using image analysis on FNAs and tissue sections on the same surgical breast specimens. This demonstrates that ploidy can be achieved on cytologic breast material and may have a role in the preoperative assessment of breast cancers.
authors.affiliation: Cytopathology and Academic Surgical Units, St. Mary's Hospital Medical School, London, UK.
authors.forename: S K
authors.initials: SK
authors.lastname: Sarker
authors.forename: R
authors.initials: R
authors.lastname: Mendiola
authors.forename: A
authors.initials: A
authors.lastname: Spigelman
authors.forename: M
authors.initials: M
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Coleman
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008867:Microtomy; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm