title: Flow cytometric analysis of serum deprivation-induced apoptosis of PC12 cells, with special reference to role of bcl-2.
abstract: To investigate the mechanism of serum deprivation-induced apoptosis in PC12 cells, we performed flow cytometry with the viable cell-specific fluorogen, fluorescein diacetate (FDA). Intact PC12 cells were positive when stained with FDA, and those cells were identical with the major population which possess larger forward and side scattered light. Apoptotic PC12 cells decreased the forward and side scatter light associated with the loss of FDA-positive cells. Nerve growth factor (NGF) and the bcl-2 protein protected the cells from serum deprivation-induced apoptosis. These data suggested that the process of apoptosis in PC12 cells can be analyzed with flow cytometry.
authors.affiliation: Institute for Protein Research, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Kubo
authors.forename: Y
authors.initials: Y
authors.lastname: Enokido
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D016895:Culture Media, Serum-Free; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
chemical_list: D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors