Identification of basic fibroblast growth factor-like immunoreactivity in panax ginseng extract: investigation of its molecular properties.
Basic fibroblast growth factor (bFGF)-like immunoreactivity was detected in extracts of Panax ginseng root by using a sensitive two-site enzyme immunoassay specific for human bFGF (hbFGF). In an investigation of the molecular properties of this bFGF-like molecule (bFGF-LI), the bFGF-LI and hbFGF were found to be equivalent with respect to antigenicity, molecular weight, isoelectric point, affinity for binding to heparin, and mitogenic activity toward BALB/c3T3 fibroblasts. The identification of this bFGF-LI molecule in Panax ginseng root helps to explain various activities of the traditional Chinese medicine ginseng.
Takei
Y
Y
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
Yamamoto
T
T
Higashira
H
H
Hayashi
K
K
D016475:3T3 Cells; D000818:Animals; D000941:Antigens / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D006493:Heparin / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007526:Isoelectric Point; D051379:Mice; D008934:Mitogens / Q000032:analysis; D008970:Molecular Weight; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal; D012680:Sensitivity and Specificity
D000941:Antigens; D008934:Mitogens; D010936:Plant Extracts; D016222:Fibroblast Growth Factor 2; D006493:Heparin