Reaction of deoxyguanosine with nitrous acid.
We reinvestigated the reaction of dGuo in the presence of nitrous acid by reversed phase HPLC (RPLC) analysis. When 10 mM deoxyguanosine (dGuo) was treated with 100 mM NaNO2 in 3.0 M acetate buffer (pH 3.7) at 37 degrees C, unknown peak appeared in large amount (yield 19.7% at 2 h, 28.7% at 6 h). The yield of this newly found compound, denoted as 1, arose with increasing H+ concentration and was maximal at 50 degrees C. 1 has small depurination rate constant. If no enzymatic repair system for 1 exists, 1 would have strong mutagenic ability.
Suzuki
T
T
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Makino
K
K
D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009153:Mutagens / Q000737:chemistry; D009608:Nitrous Acid / Q000737:chemistry*
D009153:Mutagens; D003849:Deoxyguanosine; D009608:Nitrous Acid