title: Characterization of structural proteins of Lelystad virus.
abstract: The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames that encode putative viral proteins. Synthetic polypeptides of 15 to 17 amino acids were selected from the amino acid sequences of ORFs 2 to 7 and anti-peptide sera were raised in rabbits. Using these anti-peptide sera and porcine anti-LV serum, we identified three structural proteins and assigned their corresponding genes. Virions were found to contain a nucleocapsid protein of 15 kDa (N), an unglycosylated membrane protein of 18 kDa (M), and a glycosylated membrane protein of 25 kDa (E). The N protein is encoded by ORF7, the M protein is encoded by ORF6, and the E protein is encoded by ORF5.
authors.affiliation: Institute for Animal Science and Health (ID-DLO), Department of Virology, Lelystad, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: Meulenberg
authors.forename: A
authors.initials: A
authors.lastname: Petersen-den Besten
authors.forename: E P
authors.initials: EP
authors.lastname: de Kluyver
authors.forename: R J
authors.initials: RJ
authors.lastname: Moormann
authors.forename: W M
authors.initials: WM
authors.lastname: Schaaper
authors.forename: G
authors.initials: G
authors.lastname: Wensvoort
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D002478:Cells, Cultured; D016676:Macrophages, Alveolar / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012328:RNA Viruses / Q000235:genetics* / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D013552:Swine; D014758:Viral Core Proteins / Q000096:biosynthesis; D014763:Viral Matrix Proteins / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D014771:Virion / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins