Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse.
The present study was designed to catalogue and compare nicotinic receptor subunit messages in the mammalian cochlea. Fourteen nicotinic acetylcholine receptor subunit messages were examined by polymerase chain reaction (PCR) analysis and nucleotide sequencing. Total RNA was extracted from the auditory organs of 14- to 18-day-old CBAJ mice, and mRNA was purified using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with the use of primers specific for the nucleotide sequences representing the following nicotinic receptor subunits: muscle types alpha 1, beta 1, gamma, delta and epsilon and neuronal types alpha 2,3,4,5,6,7 and beta 2,3,4. cDNA from cochlear tissue corresponding to the muscle-type receptor subunit beta 1 and to neuronal-type receptor subunits alpha 2,4,5,6 and beta 2,3 was amplified, whereas cDNA for muscle types alpha 1, gamma, delta and epsilon and neuronal types alpha 3,7 and beta 4 was not. All PCR products were homologous in nucleotide sequence to the corresponding reference cDNAs from which the primers were designed. The current results indicate that nicotinic acetylcholine receptor (nAChR) subunits that are similar or identical to the stated muscle and neuronal types are expressed in the murine cochlea. The presence of messages corresponding to the muscle-type beta 1 and neuronal-type nAChR subunits may be correlated with the atypical cholinergic response of cochlear hair cells to agonists and antagonists.
Drescher
D G
DG
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Khan
K M
KM
Green
G E
GE
Morley
B J
BJ
Beisel
K W
KW
Kaul
H
H
Gordon
D
D
Gupta
A K
AK
Drescher
M J
MJ
Barretto
R L
RL
D000818:Animals; D001483:Base Sequence; D003051:Cochlea / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011978:Receptors, Nicotinic / Q000145:classification* / Q000235:genetics*
D018076:DNA, Complementary; D011978:Receptors, Nicotinic