title: Influence of different dietary vitamin B6 supply during gravidity and lactation on total vitamin B6 concentration (pyridoxine, pyridoxal and pyridoxamine) in blood and milk.
abstract: In a two factorial trial with 80 (5 x 2 x 8) Sprague-Dawley rats weighing 257 g the influence of different dietary vitamin B6 supply on concentration in blood and milk was examined. The two factors were 5 doses of alimentary vitamin B6 supply during pregnancy (0.6, 3, 6, 18, and 180 mg vitamin B6 per kg diet) and 2 doses in lactation (3 and 6 mg vitamin B6 per kg diet). The rats were fed a semisynthetic diet. The daily food intake was 14 g during pregnancy and ad libitum during lactation. At day 7 and 13 of lactation the dams were milked and at day 14 the animals were killed by decapitation. Vitamin B6 concentration of milk at 7th and 13th day of lactation and vitamin B6 concentration of blood at 14th day of lactation were examined. The mean total vitamin B6 concentration in blood was 0.18 microgram/ml and consisted of 94.8% pyridoxal and 5.2% pyridoxamine. An elevation in vitamin B6 supply during pregnancy led to an increase in blood vitamin B6 concentration of 40% and the vitamin B6 treatment during lactation caused an increase of 50%. The mean total vitamin B6 concentration in milk at the 7th day of lactation was 0.35 microgram/ml and consisted of 79.5% pyridoxal, 15.6% pyridoxamine and 4.9% pyridoxine. An elevation in dietary vitamin B6 supply during pregnancy led to an increase in milk vitamin B6 concentration of 79% and vitamin B6 treatment during lactation led to an increase of 38%. The mean total vitamin B6 concentration in milk at the 13th day of lactation was 0.44 microgram/ml and consisted of 79.8% pyridoxal, 13.9% pyridoxamine and 6.3% pyridoxine. Milk vitamin B6 concentration was increased by 53% through the dietary supply during pregnancy and by 32% through the higher lactational supply. The experiments indicate that vitamin B6 concentration in blood and milk is a reflection of dietary supply, whereby in lactation milk concentration is less influenced than blood.
authors.affiliation: Institut für Ernährungsphysiologie, Technischen Universität München, Freising-Weihenstephan, Fed. Rep. of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Benedikt
authors.forename: D A
authors.initials: DA
authors.lastname: Roth-Maier
authors.forename: M
authors.initials: M
authors.lastname: Kirchgessner
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D005260:Female; D005527:Food, Fortified; D007774:Lactation / Q000097:blood / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011736:Pyridoxine / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011736:Pyridoxine