Targeting nucleic acid secondary structures by antisense oligonucleotides designed through in vitro selection.
Using an in vitro selection approach, we have isolated oligonucleotides that can bind to a DNA hairpin structure. Complex formation of these oligonucleotides with the target hairpin involves some type of triple-stranded structure with noncanonical interaction, as indicated by bandshift assays and footprinting studies. The selected oligomers can block restriction endonuclease cleavage of the target hairpin in a sequence-specific manner. We demonstrate that in vitro selection can extend the antisense approach to functional targeting of secondary structure motifs. This could provide a basis for interfering with regulatory processes mediated by a variety of nucleic acid structures.
Mishra
R K
RK
Institut National de la Santé et de la Recherche Médicale, Université Bordeaux II, France.
Le Tinévez
R
R
Toulmé
J J
JJ
D001665:Binding Sites; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000737:chemistry*
D016376:Oligonucleotides, Antisense