Comparison of toxicity induced by HT-2 toxin on human and rat granulo-monocytic progenitors with an in vitro model.
HT-2 toxin is a trichothecene mycotoxin occurring naturally in various agricultural products. Many in vitro studies have shown that HT-2 toxin is a major metabolite of the parent compound T-2 toxin. In man as well as in animals both toxins have been shown to cause alimentary intoxications and haematological disorders. Granulomonocytic progenitors (CFU-GM) from human umbilical cord blood and from rat bone marrow were cultured in the presence of HT-2 toxin (10(-7) M to 10(-10) M) for 14 days. The concentration-effect relationship was studied and the IC50 were determined on Days 7, 10 and 14. The IC50 was 1.8 x 10(-9) M, 3.5 x 10(-9) M and 1.8 x 10(-9) M for human cells, and 2 x 10(-9) M, 2.3 x 10(-9) M and 2.2 x 10(-9) M for rat cells. The results have been compared with previous findings for T-2 toxin. Although T-2 and HT-2 toxins had a similar IC50 on human and rat CFU-GM, the expression of their cytotoxicities was different. These findings are relevant to investigation of the cellular targets of T-2 and HT-2 in elucidating the mechanism of trichothecene haematotoxicity.
Lautraite
S
S
Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, Plouzané, France.
Parent-Massin
D
D
Rio
B
B
Hoellinger
H
H
D000818:Animals; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000633:toxicity
D013605:T-2 Toxin; C012351:HT-2 toxin