Inclusion of oxidized vegetable oil in broiler diets. Its influence on nutrient balance and on the antioxidative status of broilers.
Over a period of 4 wk, 24 10-d-old broiler hens were fed diets containing 11% vegetable oil (9% rapeseed oil, 2% soybean oil), which was added either fresh (1 meq O2/kg oil) or oxidized (156 meq O2/kg oil). The effects of the dietary treatments on nutrient digestibility were examined in a balance experiment. The antioxidative status of the animals was evaluated using plasma concentrations of thiobarbituric acid-reactive substances (TBARS), erythrocyte hemolysis in vitro, selenium-dependent and selenium-independent activity of glutathione peroxidase in liver cell cytosolic fractions, and concentrations of tocopherols and other fat-soluble compounds with antioxidative properties (lutein, beta-carotene, and retinol) in plasma and various tissues (skeletal muscle, cardiac muscle, liver, and abdominal fat). Compared to the fresh oil, the concentrations of linoleic and linolenic acid were slightly lower in oxidized oil. The concentration of alpha-tocopherol in the diet with fresh oil was an average of 80.8 mg/kg diet, whereas the diet with oxidized oil only provided 44 mg/kg. The dietary selenium content averaged 0.48 mg/kg in both diets. During the experiment, none of the animals showed symptoms of diarrhea or vitamin E deficiency. The intake of oxidized oil caused a growth depression after 2 wk. The retention of fat (P = 0.07), energy (P = 0.09), and alpha-tocopherol (P < 0.01) was lower in the group fed oxidized fat. Furthermore, these animals showed significantly higher plasma concentrations of TBARS (P < 0.01), and lower concentrations of tocopherols, lutein, beta-carotene, and retinol in plasma and tissues.
Engberg
R M
RM
Department of Nutrition, Danish Institute of Animal Science, Research Centre Foulum, Tjele, Denmark.
Lauridsen
C
C
Jensen
S K
SK
Jakobsen
K
K
D000273:Adipose Tissue / Q000737:chemistry; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002645:Chickens / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D004912:Erythrocytes / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis / Q000494:pharmacology; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D014975:Lutein / Q000032:analysis / Q000097:blood; D018482:Muscle, Skeletal / Q000737:chemistry; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology; D000074262:Rapeseed Oil; D012643:Selenium / Q000032:analysis / Q000494:pharmacology / Q000502:physiology; D013024:Soybean Oil / Q000378:metabolism / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014675:Vegetables / Q000378:metabolism* / Q000592:standards*; D014801:Vitamin A / Q000032:analysis / Q000097:blood; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology; D017962:alpha-Linolenic Acid / Q000032:analysis / Q000494:pharmacology; D019207:beta Carotene / Q000032:analysis / Q000097:blood
D000975:Antioxidants; D005229:Fatty Acids, Monounsaturated; D008041:Linoleic Acids; D010938:Plant Oils; D000074262:Rapeseed Oil; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D017962:alpha-Linolenic Acid; D014801:Vitamin A; D014810:Vitamin E; D013024:Soybean Oil; D019787:Linoleic Acid; D005979:Glutathione Peroxidase; D012643:Selenium; D014975:Lutein