Use of INAA to study the interaction between Se and Te in cells of Saccharomyces cerevisiae.
The differences in the effects of inorganic Se (IV and VI) compounds and seleno-cystine [(CySe)2] on the Te (as Na2TeO3) uptake by the yeast, Saccharomyces cerevisiae, has been studied. Se, Te, Ag, Zn, Fe and Co contents of the cells were measured by instrumental neutron activation analysis. For the determination of the Ag content, the monostandard method was applied as the analytical method. The contents of other elements were determined by comparison with standards having similar amounts of the determined element as the sample. Results obtained show that an antagonist interaction occurs between SeO2 and Te. There was a significant increase in the concentrations of Se and Te when the yeast was incubated in the medium containing (CySe)2 and Te. (CySe)2 markedly increased the Ag content of cells, especially within the first 8 h of incubation. The low level of SeO2 in the medium are the exterior factor which produce an observable increase of the Ag concentration in the cells. The higher level of SeO2 in the medium causes a long-term marked increase in the Ag content of the cells. The uptake yield of Ag also increased in the presence of (NH4)2SeO4 in the medium. The Te supply produced a significant enhancement in the Ag content of the cells during the initial 8 h of incubation. The presence of Se and/or Te in the medium causes change in the intracellular Zn, Fe and Co levels.
Czauderna
M
M
Kielanowski Institute of Animal Physiology, Warsaw, Poland.
Turska
M
M
Sierakowska
S
S
Smoliński
S
S
D003553:Cystine / Q000031:analogs & derivatives / Q000494:pharmacology; D008670:Metals / Q000378:metabolism; D009501:Neutron Activation Analysis; D016566:Organoselenium Compounds / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism*; D018036:Selenium Compounds / Q000494:pharmacology*; D012834:Silver / Q000032:analysis / Q000378:metabolism; D013691:Tellurium / Q000493:pharmacokinetics*
D008670:Metals; D016566:Organoselenium Compounds; D018036:Selenium Compounds; C009226:selenocystine; D012834:Silver; D003553:Cystine; C026660:tellurous acid; D013691:Tellurium