title: PCR-based site-directed mutagenesis.
authors.affiliation: Department of Molecular Pathology, Takara Shuzo Co., Shiga, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shimada
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant