Alternative splicing of the FMR1 gene in human fetal brain neurons.
The alternative splicing expression of the FMR1 gene was reported in several human and mouse tissues. Five regions of FMR1 gene can be alternatively spliced, but the combination of them has not been investigated fully. We reported here the analysis of alternative splicing pattern of the FMR1 gene in cultured fetal human neurons, using a RT-PCR and cloning strategy. Eleven splicing types were cloned and different isoforms were not equally represented. The dominant isoform represents nearly 40%, and the other isoforms were relatively rare. One isoform has a different carboxylterminus. Most of the alternative spliced regions appear hydrophilic; thus, they may locate on the surface of the FMR1 protein.
Huang
T
T
National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
Li
L Y
LY
Shen
Y
Y
Qin
X B
XB
Pang
Z L
ZL
Wu
G Y
GY
D017398:Alternative Splicing; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D005333:Fetus; D051860:Fragile X Messenger Ribonucleoprotein 1; D005865:Gestational Age; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D015183:Restriction Mapping
D017931:DNA Primers; D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C497683:FMR1 protein, human; C497684:Fmr1 protein, mouse