Identification of cyclic cystathionine sulfoxide and N-acetylcyclic cystathionine in the urine of a patient with cystathioninuria using liquid chromatography-mass spectrometry with an atmospheric pressure chemical ionization interface system.
Perhydro-1,4-thiazepine-4,5-dicarboxylic acid sulfoxide (cyclic cystathionine sulfoxide [cyclic cystaSO]) and N-acetylperhydro-1,4-thiazepine-3,5-dicarboxylic acid (NAc-cyclic cysta) have been identified in the urine of a patient with cystathioninuria as new metabolites of cystathionine for the first time using liquid chromatography-mass spectrometry with an atmospheric pressure chemical ionization interface system (LC/APCI-MS). The concentrations of cyclic cystaSO and NAc-cyclic cysta in the urine of a patient with cystathioninuria have also been determined for the first time using this method: 18.24 +/- 0.79 and 25.23 +/- 0.83 mg/g creatinine, respectively.
Zhang
J
J
Department of Chemistry, Kochi Medical School, Japan.
Sugahara
K
K
Sagara
Y
Y
Hashimoto
K
K
Masuoka
N
N
Kodama
H
H
D001274:Atmospheric Pressure; D002621:Chemistry / Q000379:methods; D002853:Chromatography, Liquid; D003540:Cystathionine / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D009994:Osmolar Concentration
D003540:Cystathionine; C010094:cystathionine sulfoxide