title: Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization.
abstract: We have isolated a 3.3-kb DNA containing the two exons and a 2.6-kb intron of the human CPX2 gene. This gene encodes the intestinal isoenzyme of glutathione peroxidase, GSHPx-GI. Consistent signals were detected at 14q24.1 when this DNA was used as a probe for fluorescence in situ hybridization on metaphase chromosomes. Based on either single-stranded conformation polymorphism or sequencing analysis, two sites containing DNA polymorphism were found in the intron: the 5' end had a single A/T alteration, and the 3' end had a microsatellite of TC repeats.
authors.affiliation: Department of Medical Oncology & Therapeutics Research, City of Hope Medical Center, Duarte, CA 91010, USA. fchu@smtplink.coh.org
authors.forename: F F
authors.initials: FF
authors.lastname: Chu
authors.forename: H A
authors.initials: HA
authors.lastname: Rohan de Silva
authors.forename: R S
authors.initials: RS
authors.lastname: Esworthy
authors.forename: K K
authors.initials: KK
authors.lastname: Boteva
authors.forename: C E
authors.initials: CE
authors.lastname: Walters
authors.forename: A
authors.initials: A
authors.lastname: Roses
authors.forename: P N
authors.initials: PN
authors.lastname: Rao
authors.forename: M J
authors.initials: MJ
authors.lastname: Pettenati
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004247:DNA; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D007527:Isoenzymes; D004247:DNA; D005979:Glutathione Peroxidase