Electrophoretic and immunoblot analysis of Campylobacter fetus lipopolysaccharides.
Proteinase K-digested cell lysates from 25 Campylobacter fetus subspecies fetus and C. fetus subsp. venerealis strains were examined by SDS-PAGE and immunoblotting. Three SDS-PAGE lipopolysaccharide (LPS) profiles were observed. Two profiles were consistent with those previously reported for serogroup A and serogroup B and AB isolates and were distinguished by the relative mobility of bands in the O-chain region and by a strong reaction on immunoblots with homologous antisera. The third profile was similar but had faster migrating O-chain bands. Immunoblot reactions using homologous and heterologous adsorbed antisera showed that the O-antigen of the C. fetus subsp. fetus reference strain and other profile 2-type LPS strains was distinct from the O-antigens of strains with profile 1- or profile 3-type LPS. O-antigens of strains with profile 1- and profile 3-type LPS had shared epitopes. One strain had core components but no detectable O-antigens. Common core LPS antigens appear to be present in all strains and antibodies to common core LPS epitopes may be useful reagents for rapid detection of C. fetus.
Brooks
B W
BW
Agriculture and Agri-Food Canada, Animal Diseases Research Institute, NEPEAN, Ontario, Canada. brooksb@em.agr.ca
Garcia
M M
MM
Robertson
R H
RH
Lior
H
H
D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000737:chemistry*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D008070:Lipopolysaccharides