title: Inhibition of acid production in Streptococcus mutans R9 by formic acid.
abstract: Both lactic and acetic acids cause mixed inhibition of acid production in mutans streptococci. This inhibition is partly irreversible due to cell death, an important factor when considering acidogenicity and aciduricity of these organisms, and their role in the caries process. Other monocarboxylic end-products may be present and may also be important inhibitors of acid production in dental plaque. This study considered the effects of varying concentrations of the end-product formic acid on acid production rates in Streptococcus mutans R9, measured using the pH-stat. Undissociated formic acid caused mixed inhibition with constants of Kiu (uncompetitive) of 6.07 +/- 1.27 mmol-1 and Kic (competitive) of 0.2 +/- 0.11 mmol l-1. Inhibition was found to be fully reversible, with no loss of cell viability. It is concluded that at those concentrations found in vivo, formate is not a significant inhibitor of acid production by S. mutans in dental plaque at any time, and is not important in determining the acidogenicity or aciduricity of this organism.
authors.affiliation: MRC Dental Group, Dental School, Bristol, UK. s.j.assinder@bristol.uk.ac
authors.forename: S J
authors.initials: SJ
authors.lastname: Assinder
authors.forename: H A
authors.initials: HA
authors.lastname: Popiel
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D003731:Dental Caries / Q000209:etiology; D003773:Dental Plaque / Q000378:metabolism / Q000382:microbiology; D005561:Formates / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D005561:Formates; C030544:formic acid; D019344:Lactic Acid; D019342:Acetic Acid