Evaluation of a benchtop ion trap gas chromatographic-tandem mass spectrometric instrument for the analysis of a model drug, tebufelone, in plasma using a stable-isotope internal standard.
The performance of a benchtop GC-ion trap MS-MS instrument, the Varian Saturn 4D, was evaluated for the analysis of a model drug, tebufelone, in plasma. The sample preparation scheme was designed to provide a highly complex extract with matrix-derived interferences eluting near and at the retention time of tebufelone and its stable-isotope-labeled analog. The performance of the ion trap in the selected-reaction-monitoring mode was evaluated and also compared with results obtained on a benchtop GC-MS linear quadrupole instrument operated in the selected-ion-monitoring mode. The ion trap, operated in the selected-reaction-monitoring mode, was found to provide a higher degree of selectivity for the analysis of tebufelone. The increased selectivity obtained on the ion trap operated in the selected-reaction-monitoring mode resulted in superior accuracy and precision, as well as a lower limit of quantitation relative to that obtained by the GC-MS analysis. A linear standard curve was obtained over three orders of magnitude and the limit of quantitation for tebufelone in plasma was 100 pg/ml using the GC-ion trap MS-MS instrument.
Wehmeyer
K R
KR
Miami Valley Laboratories, Procter and Gamble Pharmaceuticals, Cincinnati, OH 45253-8707, USA.
Knight
P M
PM
Parry
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RC
D000480:Alkynes / Q000097:blood*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002247:Carbon Isotopes; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation*; D007202:Indicators and Reagents; D010103:Oxygen Isotopes; D010636:Phenols / Q000097:blood*; D011817:Rabbits; D012015:Reference Standards
D000480:Alkynes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002247:Carbon Isotopes; D007202:Indicators and Reagents; D010103:Oxygen Isotopes; D010636:Phenols; C064268:tebufelone