title: A low molecular weight urokinase derivative with enhanced fibrin affinity.
abstract: A Gly-Pro-Arg-Pro tetrapeptide, homologous to amino-terminal segment of the human fibrin alpha chain after the release of the fibrinopeptide A, was covalently coupled to peptide A of low molecular weight urokinase. The resulting derivative gained increased affinity for fibrin. In caseinolytic assay, fibrin can stimulate the derivative to activate plasminogen. The derivative had two-fold greater fibrinolytic potency than native low molecular weight urokinase and its affinity for fibrin clot was 3.9-fold higher than that of low molecular weight urokinase.
authors.affiliation: Department of Biochemistry, Nanjing University, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Chen
authors.forename: Z C
authors.initials: ZC
authors.lastname: Hua
authors.forename: D X
authors.initials: DX
authors.lastname: Zhu
mesh_terms: D002846:Chromatography, Affinity; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D008970:Molecular Weight; D009842:Oligopeptides / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009842:Oligopeptides; C034585:glycyl-prolyl-arginyl-proline; D005337:Fibrin; D014568:Urokinase-Type Plasminogen Activator