Repetitive element sequence based polymerase chain reaction for typing of Brucella strains.
A collection of 40 Brucella strains, including 19 clinical B.canis isolates, was analyzed using ERIC-PCR and REP-PCR. PCR amplification using primers REP 1R-I and REP 2-I provided distinct patterns for Brucella spp. All Brucella strains could be discriminated at least to the species level. ERIC-PCR, employing ERIC 1R and ERIC 2 as primers was less discriminative for Brucella, only allowing a discrimination to the genus level with occasional discrimination between individual strains. It can be concluded that rep PCR is a promising fingerprinting method for the evaluation of an outbreak.
Tcherneva
E
E
Central Veterinary Research Institute, Sofia, Bulgaria.
Rijpens
N
N
Naydensky
C
C
Herman
L
L
D000818:Animals; D001483:Base Sequence; D002002:Brucella / Q000145:classification*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary*; D016172:DNA Fingerprinting / Q000662:veterinary; D004269:DNA, Bacterial / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012230:Rhizobiaceae / Q000145:classification
D004269:DNA, Bacterial