DNA ploidy as a prognostic factor for patients with an adenocarcinoma in Barrett's esophagus.
OBJECTIVE
The role of DNA ploidy of tumor cells as a prognostic factor for survival after resection was evaluated in 40 patients with an adenocarcinoma in Barrett's esophagus (28 men and 12 women; mean age, 61 years).


METHODS
After resection of the esophagus, staging was performed according to the TNM-classification (UICC 1987). Tumor tissue specimens were reviewed for the histological differentiation grade and flow cytometry was performed on paraffin embedded tissue according to the method described by Hedley.


RESULTS
There was no significant correlation between DNA-ploidy and TNM-stage or histological differentiation grade of the tumor. DNA ploidy, stage and grade as prognostic factors for the survival were evaluated by multivariate analysis. DNA ploidy significantly (p= 0.04) correlated with survival, also univariately. Patients with diploid tumors had 3- and 5-year survival rates of 51% and 25% respectively, as compared to 10% and 0% respectively for patients with aneuploid or tetraploid/increased G2 tumors.


CONCLUSIONS
DNA ploidy is an independent prognostic factor for the survival of patients with an adenocarcinoma in Barrett's esophagus.
Menke-Pluymers
M B
MB
Rotterdam Esophageal Tumor Study Group Department of Surgery, University Hospital Rotterdam-Dijkzigt, The Netherlands.
Hop
W C
WC
Mulder
A H
AH
Tilanus
H W
HW
D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D001471:Barrett Esophagus / Q000150:complications*; D004273:DNA, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000150:complications / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011003:Ploidies; D011379:Prognosis; D016019:Survival Analysis
D004273:DNA, Neoplasm