title: Somatostatin receptors in the developing rat brain.
abstract: The distribution of [125I]SRIF-28 ([Leu8,D-Trp22,125I-Tyr25]somatostatin-28), [125I]204-090 ([Tyr3]octreotide) and [125I]CGP 23996 (c[Asu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Tyr-Thr-Ser]) labelled recognition sites was studied by autoradiography in rat brain at embryonic day 18 (E 18) and postnatal day 5 (P 5). These results were compared with mRNA expression of somatostatin receptors SSTR1-5 (named sst1-5 now) as studied by in situ hybridization. [125I]SRIF-28, [125I]204-090 and [125I]CGP 23996 binding displayed different although partially overlapping distributions, and showed an increase between E 18 and P 5, which was less marked for [125I]204-090 binding. -125I-204-090 binding and sst2 receptor mRNA were similarly distributed, whereas [125I]CGP 23996 binding did not correlate with any single somatostatin receptor mRNA. The data suggest that most SRIF receptor subtypes in rat brain are present before birth, but evolve differently.
authors.affiliation: Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
authors.forename: V S
authors.initials: VS
authors.lastname: Thoss
authors.forename: D
authors.initials: D
authors.lastname: Duc
authors.forename: D
authors.initials: D
authors.lastname: Hoyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006728:Hormones / Q000494:pharmacology; D017403:In Situ Hybridization; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D015282:Octreotide / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000187:drug effects / Q000378:metabolism*; D013004:Somatostatin / Q000031:analogs & derivatives / Q000494:pharmacology; D053561:Somatostatin-28
chemical_list: D006728:Hormones; D007457:Iodine Radioisotopes; D017481:Receptors, Somatostatin; D013004:Somatostatin; D053561:Somatostatin-28; C038136:CGP 23996; D015282:Octreotide