Molecular characterization of Chlorella chromosomes: screening of bent DNAs.
As a model for plant-type chromosomes, we have been characterizing molecular organization of Chlorella vulgaris (C-169) chromosome I. To locate structurally important chromosome regions, bent DNA fragments of this chromosome were systematically obtained and analyzed. By two-dimensional polyacrylamide gel electrophoresis, 150 clones containing bent DNA fragments were screened from C. vulgaris genomic libraries. These clones were classified into 3 groups by Southern hybridization. A group that hybridized with all Chlorella chromosomes contained fragments carrying a retroposon-like element. A clone (p2DS-66A) derived from chromosome VI strongly hybridized with chromosome I as well as the others. The corresponding chromosome I sequences (pI-21 and pI-31) were analyzed in detail.
Akiba
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Noutoshi
Y
Y
Maki
S
S
Higashiyama
T
T
Yamada
T
T
D001483:Base Sequence; D002708:Chlorella / Q000737:chemistry* / Q000235:genetics*; D002875:Chromosomes / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018626:Retroelements
D018626:Retroelements; D004247:DNA