Elicitors triggering the simultaneous gene expression of antibacterial proteins of the silkworm, Bombyx mori.
Various elicitors were examined by Northern blot analysis to investigate the simultaneous induction of gene expression of antibacterial proteins such as cecropin B, attacin and lebocin from the silkworm, Bombyx mori. Lipopolysaccharide (LPS), lipid A, 2-keto-3-deoxyoctonate (KDO) and peptidoglycan (PG) triggered efficiently and simultaneously the gene expression of antibacterial proteins. Effects of inhibitors for signal transduction on the gene expression of Bombyx mori (Bm) cecropin B triggered by lipid A were observed using isolated adherent hemocytes consisting of granular cells and plasma cells. H-7, H-89 but not W-7 inhibited gene expression, suggesting that protein kinase C and A but not myosine light chain kinase may participate in signal transduction.
Taniai
K
K
Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
Furukawa
S
S
Shono
T
T
Yamakawa
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents; D012831:Bombyx / Q000378:metabolism*; D002448:Cell Adhesion; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D017344:Genes, Insect; D006434:Hemocytes / Q000187:drug effects / Q000502:physiology; D007301:Insect Hormones / Q000096:biosynthesis*; D019476:Insect Proteins; D007546:Isoquinolines / Q000494:pharmacology; D007814:Larva; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology; D014176:Protein Biosynthesis; D015398:Signal Transduction; D013400:Sugar Acids / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology
D000890:Anti-Infective Agents; D004791:Enzyme Inhibitors; D007301:Insect Hormones; D019476:Insect Proteins; D007546:Isoquinolines; D008050:Lipid A; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D013400:Sugar Acids; D013449:Sulfonamides; C039711:attacin antibacterial protein, insect; C095582:lebocin protein, Bombyx mori; C002532:2-keto-3-deoxyoctonate; C017967:W 7; C031162:cecropin B protein, Insecta; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide