Synthesis of elastin by pleomorphic adenomas.
Previous morphologic and histochemical studies have documented extracellular elastin and elastic fibers within the matrix of pleomorphic adenomas of the salivary glands. By morphologic criteria, the elastin appeared to be synthesized by the tumor rather than being a consequence of stroma produced in response to the tumor cells. To examine this issue, nine pleomorphic adenomas were studied by immunohistochemistry and in situ hybridization with tropoelastin-specific antibodies and complementary DNAs. Corroborating the previous morphologic studies, immunohistochemistry demonstrated abundant extracellular elastin within the matrix of pleomorphic adenomas. Additionally, both immunohistochemistry and in situ hybridization demonstrated continued synthesis of tropoelastin by the neoplastic cells. Tropoelastin production was seen in neoplastic cells with all morphologies.
Grosso
L E
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Department of Pathology, St. Louis University Medical Center, Missouri, USA.
D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D018076:DNA, Complementary; D004549:Elastin / Q000032:analysis / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007633:Keratins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000378:metabolism* / Q000473:pathology; D009418:S100 Proteins / Q000032:analysis; D014333:Tropoelastin / Q000032:analysis / Q000096:biosynthesis
D000906:Antibodies; D018076:DNA, Complementary; D009418:S100 Proteins; D014333:Tropoelastin; D007633:Keratins; D004549:Elastin