Expression and regulation of prostaglandin F receptor mRNA in rodent osteoblastic cells.
The ability of prostaglandins to inhibit collagen synthesis and induce prostaglandin G/H synthase in bone cells appears to be mediated by the prostaglandin F2 alpha receptor (FPR). We have identified FPR mRNA in the osteoblastic cell lines, Py1a from rats and MC3T3-E1 from mice, as well as in the stem cell cultures, MN-7 and mouse marrow, using reverse transcriptase-polymerase chain reaction technology (RT-PCR). Expression of FPR mRNA was increased in Pyla, MN-7 and marrow cells with prolonged culture or dexamethasone treatment and decreased after treatment with fluprostenol, a selective FPR agonist.
Nemoto
K
K
Department of Medicine, University of Connecticut Health Center, Farmington, USA.
Bernecker
P M
PM
Pilbeam
C C
CC
Raisz
L G
LG
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001842:Bone and Bones / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000235:genetics*; D008186:Luteolytic Agents / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000819:agonists / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D008186:Luteolytic Agents; D011461:Prostaglandins F, Synthetic; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; C006326:fluprostenol; D003907:Dexamethasone; D015237:Dinoprost