Determination of paclitaxel in biological fluids by micellar electrokinetic chromatography.
A method has been developed for the determination of paclitaxel (Taxol) in plasma and urine using capillary electrophoresis with sodium dodecyl sulfate as additive in the run buffer. The samples are extracted and preconcentrated with tert.-butyl methyl ether. Taxotere has been used as the internal standard. The limit of detection of paclitaxel is 20 ng/ml. In comparison to high-performance liquid chromatography, the capillary electrophoresis method is simple and needs less organic solvents.
Hempel
G
G
Universitäts-Kinderklinik, Abt. Hämatologie/Onkologie, Münster, Germany.
Lehmkuhl
D
D
Krümpelmann
S
S
Blaschke
G
G
Boos
J
J
D000097:Acetonitriles / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000737:chemistry; D000077143:Docetaxel; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008823:Micelles; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D043823:Taxoids
D000097:Acetonitriles; D000972:Antineoplastic Agents, Phytogenic; D008823:Micelles; D043823:Taxoids; D000077143:Docetaxel; D012967:Sodium Dodecyl Sulfate; D017239:Paclitaxel; C032159:acetonitrile