Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum.
Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty-eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.
Ang
H H
HH
School of Pharmaceutical Sciences, University of Science Malaysia, Minden, Filaria, Malaysia.
Chan
K L
KL
Mak
J W
JW
D000818:Animals; D004586:Electrophoresis; D005838:Genotype; D018086:Glutamate Dehydrogenase (NADP+) / Q000145:classification / Q000302:isolation & purification*; D007527:Isoenzymes / Q000145:classification / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics
D007527:Isoenzymes; D018086:Glutamate Dehydrogenase (NADP+)