Study of JA300 as agent having high transglycosylation activity.
Escherichia coli JA300 as the strain catalyzing transglycosylation of nucleic acids has so strong activity. We scrutinized where the high faculty of this strain come from. Using PCR methods with our primer pair MI-1 (up; GTT GAA TTC GCC TTT GTT ATG TCA C) and MI-2 (down; GTT CCG CTA ACG GAA CAC CTA GGC CT), we isolated deoD (the coding region of PNPase) of JA300. Nucleotide sequence analysis indicated that deoD of JA300 is definitely same as that of parent strain K12. The fact that the transcription of deoD of JA300 is more active than that of K12 was also revealed after the analysis by Northrn blot hybridization. These data may suggest that the varieties of gene concerning regulation contribute to the difference of PNPase activity between them.
Izumi
M
M
Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd., Yamaguchi, Japan.
Kojima
E
E
Ishida
S
S
Kaneko
R
R
Miyawaki
S
S
Kawaoka
A
A
Machida
M
M
Murakami
K
K
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D006031:Glycosylation; D011683:Purine-Nucleoside Phosphorylase / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D012333:RNA, Messenger; D011683:Purine-Nucleoside Phosphorylase