Selective labeling of [3H]2-deoxy-D-glucose in the snake trigeminal system: basal and infrared-stimulated conditions.
[3H]2-Deoxy-D-glucose (2-DG) and high-resolution autoradiography were employed to investigate labeling patterns of the trigeminal and infrared sensory system in a crotaline snake, the pit viper (Trimeresurus flavoviridis). Following intracardiac injection of 9.25 MBq [3H]2-DG, neurons in the nucleus of the lateral descending trigeminal tract (LTTD), nucleus reticularis caloris (RC), nucleus trigemini mesencephalicus, nucleus trigemini motorius, and trigeminal ganglia were labeled in various degrees after the pit organ had been removed (basal condition). This revealed that a higher rate of glucose utilization occurred in these nuclei than in the common sensory trigeminal nuclei, which lacked labeling entirely. When a pit was stimulated periodically with an infrared stimulus for 45 min, the difference in percentage of labeled cells was ipsilaterally increased by 12.84% in large cells of the LTTD and by 7.55% in the RC, as compared with the contralateral, basal-condition side. These slight changes indicate a small increase of glucose consumption during infrared reception. On the other hand, the small cells in the LTTD showed labeling that did not change with stimulation, suggesting that 2-DG uptake in inhibitory interneurons is relatively constant.
Jiang
P J
PJ
Department of Physiology, University of the Ryukyus School of Medicine, Okinawa, Japan.
Terashima
S
S
D000344:Afferent Pathways / Q000033:anatomy & histology / Q000502:physiology; D000818:Animals; D001345:Autoradiography; D001786:Blood Glucose / Q000378:metabolism*; D001931:Brain Mapping; D003847:Deoxyglucose / Q000378:metabolism; D004292:Dominance, Cerebral / Q000502:physiology; D007259:Infrared Rays; D007395:Interneurons / Q000502:physiology / Q000648:ultrastructure; D009433:Neural Inhibition / Q000502:physiology; D011984:Sensory Receptor Cells / Q000033:anatomy & histology / Q000502:physiology*; D014276:Trigeminal Nerve / Q000033:anatomy & histology / Q000502:physiology*; D014278:Trigeminal Nuclei / Q000033:anatomy & histology / Q000502:physiology*; D017819:Viperidae / Q000033:anatomy & histology / Q000502:physiology*
D001786:Blood Glucose; D003847:Deoxyglucose