title: Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect.
abstract: To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug: repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.
authors.affiliation: Department of Clinical Microbiology and Antimicrobial Therapy, Erasmus University Rotterdam, Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: den Hollander
authors.forename: J W
authors.initials: JW
authors.lastname: Mouton
authors.forename: I A
authors.initials: IA
authors.lastname: Bakker-Woudenberg
authors.forename: F P
authors.initials: FP
authors.lastname: Vleggaar
authors.forename: M P
authors.initials: MP
authors.lastname: van Goor
authors.forename: H A
authors.initials: HA
authors.lastname: Verbrugh
mesh_terms: D000123:Acetyltransferases / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D005839:Gentamicins / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014031:Tobramycin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D014031:Tobramycin