Induction of muscarinic receptor subtypes in monocytic/macrophagic cells differentiated from EoL-1 cells.
We demonstrated that eosinophilic leukemia cell line-1 (EoL-1 cells) differentiated into monocytic/macrophagic cells by a treatment with interferon-gamma. Muscarinic receptor mRNA was not detected in untreated EoL-1 cells, and the cytosolic concentration of Ca2+ ([Ca2+]i) did not rise either in these cells. Interestingly, when EoL-1 cells were treated with interferon-gamma, mRNAs for muscarinic M3 and M5 receptors could be detected in these cells, along with an increase in [Ca2+]i and chemotaxis induced by carbachol that could be blocked with 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. These data support the functional importance of muscarinic M3 and M5 receptors in monocytic/macrophagic cells differentiated from EoL-1 cells. This model also provides evidence of a significant functional interaction between muscarinic M3 and M5 receptors.
Mita
Y
Y
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Dobashi
K
K
Suzuki
K
K
Mori
M
M
Nakazawa
T
T
D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002454:Cell Differentiation; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D018721:Muscarinic Agonists / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011976:Receptors, Muscarinic / Q000096:biosynthesis / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D018721:Muscarinic Agonists; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002217:Carbachol; D002118:Calcium