Mammalian expression of single chain variable region fragments dimerized by Fc regions.
To promote application of a single chain variable region fragment (sFv) in immunoglobulins, a sFv gene was connected to an IgGi Fc gene, designated as a sFvc gene, and used for transfection of Sp2/0. As a result, the sFvc protein was found to be secreted in a dimeric form. It is thus felt that the sFvc protein, which mimicks the shape of a naturally occurring antibody, can be simple and useful to reproduce divalency and Fc-associated effecter functions as seen in a natural antibody.
Kato
T
T
Institute of Medical Science, St. Marianna University School of Medicine, Japan.
Sato
K
K
Suzuki
S
S
Sasakawa
H
H
Kurokawa
M
M
Nishioka
K
K
Yamamoto
K
K
D000818:Animals; D003001:Cloning, Molecular; D019281:Dimerization; D005803:Genes, Immunoglobulin / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D007141:Immunoglobulin Fc Fragments; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D011994:Recombinant Proteins; C055966:immunoglobulin Fv