title: Regulation of transcription of coronaviruses.
abstract: To study factors involved in regulation of transcription of coronaviruses, we constructed defective interfering (DI) RNAs containing sg RNA promoters at multiple positions. Analysis of the amounts of sg DI RNA produced by these DIs resulted in the following observations: (i) a downstream promoter downregulates an upstream promoter; (ii) an upstream promoter has little or no effect on the activity of a downstream promoter. Our data suggest that attenuation of upstream promoter activities by downstream promoter sequences plays an important role in regulating the amounts of sg RNAs produced by coronaviruses. Our observations are in accordance with the models proposed by Konings et al. and Sawicki and Sawicki.
authors.affiliation: Department of Virology, Faculty of Medicine, Leiden University, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: van Marle
authors.forename: R G
authors.initials: RG
authors.lastname: van der Most
authors.forename: T
authors.initials: T
authors.lastname: van der Straaten
authors.forename: W
authors.initials: W
authors.lastname: Luytjes
authors.forename: W J
authors.initials: WJ
authors.lastname: Spaan
mesh_terms: D000818:Animals; D001483:Base Sequence; D017934:Coronavirus / Q000235:genetics* / Q000378:metabolism; D003673:Defective Viruses / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral