A radiation hybrid map spanning the entire human X chromosome integrating YACs, genes, and STS markers.
We present a radiation hybrid (RH) map of human Chromosome (Chr) X, using 50 markers on 72 radiation hybrids. The markers, obtained from the consensus map, form a grid spanning the entire chromosome. To check the RH map, the marker order was determined by analysis of presence or absence of retained human DNA fragments in the RHs; the comparison with the consensus showed a similar order. Any STSs, microsatellites, genes, and clones can be positioned and ordered relative to the marker grid. This approach integrates genetic, physical, and large-scale clone mapping and is used to link YAC contigs containing data from various experimental sources.
Kumlien
J
J
Department of Genome Analysis, Imperial Cancer Research Fund, P.O. Box 123, 44 Lincoln's Inn Fields, London WC2A 3 PX, UK.
Grigoriev
A
A
Roest Crollius
H
H
Ross
M
M
Goodfellow
P N
PN
Lehrach
H
H
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D016324:Sequence Tagged Sites; D014960:X Chromosome
D005819:Genetic Markers