title: The nuclear hormone receptor coactivator SRC-1 is a specific target of p300.
abstract: p300 and its family member, CREB-binding protein (CBP), function as key transcriptional coactivators by virtue of their interaction with the activated forms of certain transcription factors. In a search for additional cellular targets of p300/CBP, a protein-protein cloning strategy, surprisingly identified SRC-1, a coactivator involved in nuclear hormone receptor transcriptional activity, as a p300/CBP interactive protein. p300 and SRC-1 interact, specifically, in vitro and they also form complexes in vivo. Moreover, we show that SRC-1 encodes a new member of the basic helix-loop-helix-PAS domain family and that it physically interacts with the retinoic acid receptor in response to hormone binding. Together, these results implicate p300 as a component of the retinoic acid signaling pathway, operating, in part, through specific interaction with a nuclear hormone receptor coactivator, SRC-1.
authors.affiliation: Dana-Farber Cancer Institute, Boston, MA, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: Yao
authors.forename: G
authors.initials: G
authors.lastname: Ku
authors.forename: N
authors.initials: N
authors.lastname: Zhou
authors.forename: R
authors.initials: R
authors.lastname: Scully
authors.forename: D M
authors.initials: DM
authors.lastname: Livingston
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D050881:E1A-Associated p300 Protein; D019084:Fluorescent Antibody Technique, Indirect; D051548:Histone Acetyltransferases; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D056920:Nuclear Receptor Coactivator 1; D011485:Protein Binding; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D018168:Receptors, Retinoic Acid; D015534:Trans-Activators; D014157:Transcription Factors; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; D051548:Histone Acetyltransferases; C538861:Ncoa1 protein, mouse; D056920:Nuclear Receptor Coactivator 1