Targeted infection of a retrovirus bearing a CD4-Env chimera into human cells expressing human immunodeficiency virus type 1.
We constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficiently as the wild-type MoMLV Env. The hybrid MoMLV could infect human HeLa cells (although not with high efficiency) only if the cells were expressing human immunodeficiency virus type 1 genome. This method of ligand incorporation into a virion may lead to a development of a cell-specific retroviral vector for targeting gene therapy.
Matano
T
T
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Odawara
T
T
Iwamoto
A
A
Yoshikura
H
H
D016475:3T3 Cells; D000818:Animals; D015704:CD4 Antigens / Q000235:genetics*; D002462:Cell Membrane / Q000821:virology; D005622:Friend murine leukemia virus / Q000235:genetics; D015870:Gene Expression; D015686:Gene Products, env / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D014771:Virion / Q000737:chemistry
D015704:CD4 Antigens; D015686:Gene Products, env; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins