Elevated levels of heat-shock protein 70 (HSP70) in the mononuclear cells of patients with non-insulin-dependent diabetes mellitus.
Increased free radicals and reduced levels of antioxidants have been reported in diabetes mellitus. Since heat-shock protein 70 (HSP70) is a stress-induced protein and is suggested to play a protective role against oxidative stress, we have investigated whether HSP7O acts as one of the defense systems against this stress. We separated mononuclear cells from diabetic patients (N=12) and age- and sex-matched healthy controls (N =12), and detected HSP70 by western blot analysis. The results were expressed by the ratio of the density determined by laser densitometry, to that of 10 mu g of purified HSP70. HSP70 levels in the mononuclear cells of diabetic patients (0.78 + or- 0.56) were significantly high, compared with healthy subjects (0.43 + or - 0.23) (P < 0.05). No correlation was found between HSP7O and hemoglobin A1c, fasting plasma glucose, duration of diabetes or diabetic complications except age. A negative correlation was found between HSP70 and age (r = -0.658, P < 0.05). These results suggest that HSP70 levels in the mononuclear cells are elevated reflecting increased oxidative stress in patients with diabetes.
Yabunaka
N
N
Department of Internal Medicine, Noboribetsu Branch Hospital, Japan.
Ohtsuka
Y
Y
Watanabe
I
I
Noro
H
H
Fujisawa
H
H
Agishi
Y
Y
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D003720:Densitometry; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000276:immunology; D008297:Male; D051379:Mice; D008875:Middle Aged; D018384:Oxidative Stress / Q000276:immunology / Q000502:physiology*; D012016:Reference Values
D000911:Antibodies, Monoclonal; D018840:HSP70 Heat-Shock Proteins