Effect of diabetic sera on the conversion of eicosapentaenoic acid (EPA) to prostaglandin I3 by cultured bovine aortic endothelial cells.
The effects of sera from diabetic patients and healthy donors on the synthesis of prostaglandin I2 (PGI2) and PGI3 in vitro were studied in confluent bovine aortic endothelial cells cultured with EPA. The products 6-keto-PGF1 alpha and delta 17-6-keto-PGF1 alpha were measured by GC/SIM as markers of PGI2 and PGI3 formation in growth medium after 60 min of incubation. PGI2 and PGI3 synthesis with 10% diabetic sera were less than with sera from healthy donors (p < 0.05). However, the total prostacyclin production (PGI2 and PGI3) in the cell cultures incubated with 10 microM EPA and 10% diabetic sera approximated that of the cultures incubated with the sera of healthy donors without EPA. These results suggest that the diabetic sera inhibits PGI2 and PGI3 synthesis in the cultured endothelial cells, and that EPA intake may reduce the complications of diabetes mellitus, such as microangiopathy and vaso-occlusive diseases, and enhances the production of PGI3 which seems to exert a strong anti-aggregatory effect.
Mizugaki
M
M
Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan.
Nishikawa
M
M
Hishinuma
T
T
Uyama
T
T
Suzuki
K
K
Toyoda
T
T
D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D005227:Fatty Acids; D015121:6-Ketoprostaglandin F1 alpha; C034240:prostaglandin I3; D015118:Eicosapentaenoic Acid; D011464:Epoprostenol