title: Characterization of beta-lactamase gene blaPER-2, which encodes an extended-spectrum class A beta-lactamase.
abstract: Plasmidic extended-spectrum beta-lactamases of Ambler class A are mostly inactive against ceftibuten. Salmonella typhimurium JMC isolated in Argentina harbors a bla gene located on a plasmid (pMVP-5) which confers transferable resistance to oxyiminocephalosporins, aztreonam, and ceftibuten. The beta-lactamase PER-2 (formerly ceftibutenase-1; CTI-1) is highly susceptible to inhibition by clavulanate and is located at a pI of 5.4 after isoelectric focusing. The blaPER-2 gene was cloned and sequenced. The nucleotide sequence of a 2.2-kb insert in vector pBluescript includes an open reading frame of 927 bp. Comparison of the deduced amino acid sequence of PER-2 with those of other beta-lactamases indicates that PER-2 is not closely related to TEM or SHV enzymes (25 to 26% homology). PER-2 is most closely related to PER-1 (86.4% homology), an Ambler class A enzyme first detected in Pseudomonas aeruginosa. An enzyme with an amino acid sequence identical to that of PER-1, meanwhile, was found in various members of the family Enterobacteriaceae isolated from patients in Turkey. Our data indicate that PER-2 and PER-1 represent a new group of Ambler class A extended-spectrum beta-lactamases. PER-2 so far has been detected only in pathogens (S. typhimurium, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis) isolated from patients in South America, while the incidence of PER-1-producing strains so far has been restricted to Turkey, where it occurs both in members of the family Enterobacteriaceae and in P. aeruginosa.
authors.affiliation: Max von Pettenkofer-Institut, Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bauernfeind
authors.forename: I
authors.initials: I
authors.lastname: Stemplinger
authors.forename: R
authors.initials: R
authors.lastname: Jungwirth
authors.forename: P
authors.initials: P
authors.lastname: Mangold
authors.forename: S
authors.initials: S
authors.lastname: Amann
authors.forename: E
authors.initials: E
authors.lastname: Akalin
authors.forename: O
authors.initials: O
authors.lastname: Anğ
authors.forename: C
authors.initials: C
authors.lastname: Bal
authors.forename: J M
authors.initials: JM
authors.lastname: Casellas
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D000077722:Ceftibuten; D002511:Cephalosporins / Q000378:metabolism; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004269:DNA, Bacterial; D001618:beta-Lactamases; D000077722:Ceftibuten