title: The cold-shock response in bacteria.
abstract: The 'cold shock' response that occurs when exponentially growing Escherichia coli at 37 degrees C are transferred to 10 degrees C leads to the cesation of most protein synthesis, however, about 14 'cold shock' proteins continue to be made. These 'cold shock' proteins facilitate growth at low temperatures. Central to the regulation of this switch in gene expression is the 200 fold induction in the relative rate of synthesis of a small cold-shock protein only 70 amino acids in length, known as CS7.4. There is a remarkable conservation through evolution of the primary sequence found in this small cold shock protein and that of a nucleic acid binding domain within the Y-box family of eukaryotic gene regulatory proteins. Parallel studies on CS7.4 and the Y-box proteins have elucidated both molecular mechanisms regulating the cold shock response and a novel site for the regulation of eukaryotic gene expression.
authors.affiliation: Section on Molecular Biology, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Wolffe
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D051840:Y-Box-Binding Protein 1
chemical_list: D001426:Bacterial Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C062260:cold shock protein CS7.4, Bacteria