title: Sensitive detection of near-infrared fluorescent dyes using high-performance liquid chromatography with peroxyoxalate chemiluminescence detection system.
abstract: The highly sensitive detection of four near-infrared (near-IR) fluorescent dyes, methylene blue, pyridine 1, oxazine 1 and 3,3'-diethylthiadicarbocyanine iodide (DTDCI) by high-performance liquid chromatography (HPLC) with post-column peroxyoxalate chemiluminescence (PO-CL) detection utilizing bis(4-nitro-2-(3, 6,9-trioxadecyloxycarbonyl)phenyl) oxalate (TDPO) and hydrogen peroxide was examined. The detection limits for methylene blue, pyridine 1, oxazine 1 and DTDCI were 120, 27, 31, 0.19 fmol on-column at a signal-to-noise ratio of 2, respectively. The sensitivity for DTDCI was 250 times that obtained by HPLC with the conventional fluorescent detection.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kimoto
authors.forename: R
authors.initials: R
authors.lastname: Gohda
authors.forename: K
authors.initials: K
authors.lastname: Murayama
authors.forename: T
authors.initials: T
authors.lastname: Santa
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: H
authors.initials: H
authors.lastname: Homma
authors.forename: K
authors.initials: K
authors.lastname: Imai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004225:Dithiazanine / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D007259:Infrared Rays; D008163:Luminescent Measurements; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D010070:Oxalates / Q000737:chemistry; D011726:Pyridinium Compounds / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: C103429:3-(4-(4-(dimethylamino)phenyl)-1,3-butadienyl)-N-ethylpyridinium; D005456:Fluorescent Dyes; D010070:Oxalates; D011726:Pyridinium Compounds; C044622:peroxyoxalate; C103428:3,7-bis(diethylamino)phenothiazin-5-ium; D004225:Dithiazanine; D008751:Methylene Blue