A pilot study of blood antioxidant and free radical marker profiles in patients awaiting coronary artery bypass grafting.
Coronary artery bypass grafting (CABG) carries a high risk of acute pancreatitis. We report a pilot study to investigate whether pre-existing oxidative stress might underlie this susceptibility, in that a burst of free radical activity not only accompanies the reperfusion stage of CABG but seems to be a pivotal step in the pathogenesis of pancreatitis. Samples of peripheral venous blood were obtained on the morning of surgery from 8 consecutive patients (age, median and range, 62, 35-70 years) with > 75% stenosis in at least three coronary vessels and a further 8 (64, 49-70 years) who had received 1200 mg allopurinol in divided doses in the previous 48 h: the results were compared with profiles of 8 healthy controls (56, 50-60 years) with normal exercise ECG. None of the patients or controls currently smoked cigarettes and the majority drank alcohol on a social basis. Compared with controls, untreated patients had lower levels of glutathione (P < 0.001) and ascorbate (P < 0.05) in plasma, alpha-tocopherol (vitamin E as molar ratio of cholesterol, P < 0.025 and beta-carotene (P < 0.05) in serum. There was no difference in serum selenium levels, but values in patients and controls were lower than in younger controls from this area (P < 0.02). Samples from the patients contained higher concentrations of lipid peroxides than control samples (P < 0.25) but there was no evidence of excessive isomerisation of linoleic acid or oxidation of ascorbate and erythrocytes showed normal ATP and energy charge with no increase in membrane lipid peroxidisability. Treatment with allopurinol did not alter this pattern, such that the ratio of oxidised to total glutathione in plasma was higher among the 16 patients than 8 controls (P < 0.025). Habitually inadequate intakes are the best explanation for the patients' deficits in aqueous phase antioxidants; prescribed low cholesterol diets would exacerbate any prior insufficiency of lipid-phase antioxidants. Correction of these deficits during the months leading up to surgery should reduce the risk of CABG-induced acute pancreatitis.
Gu
M
M
Department of Medicine, Royal Infirmary, Manchester, UK.
Love
H
H
Schofield
D
D
Turkie
W
W
Odom
N
N
Braganza
J M
JM
D000328:Adult; D000368:Aged; D000493:Allopurinol / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D001026:Coronary Artery Bypass; D005609:Free Radicals; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D005609:Free Radicals; D014527:Uric Acid; D000493:Allopurinol