title: Activities of glycogen phosphorylase, alanine aminotransferase and aspartate aminotransferase in adult worms of Litomosoides carinii recovered from pyridoxine deficient cotton rats (Sigmodon hispidus).
abstract: This paper demonstrates that the activities of glycogen phosphorylase (GP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are reduced in adult worms of the filarial nematode Litomosoides carinii recovered from pyridoxine-deficient cotton rats when compared to worms recovered from pyridoxine-sufficient controls. GP, ALT and AST activities were determined in adult worms L. carinii recovered from cotton rat hosts over a 20-week experimental period. Activities of GP, ALT and AST in the parasite showed a direct correlation with the dietary pyridoxine intake of their host. Throughout the experiment, enzyme activities were significantly lower (P < 0.001) in worms from rats fed a pyridoxine-free diet ad libitum that in worms from rats fed either a stock colony diet, a pyridoxine-free diet ad libitum with daily supplementation of 100 micrograms pyridoxine or limited amounts of pyridoxine-free diet with daily supplementation of 100 micrograms pyridoxine. The lower than normal activity of GP, ALT, AST and other enzymes dependent on the biologically active derivative of pyridoxine, the coenzyme pyridoxal-5-phosphate (PLP), interferes with the protein, carbohydrate and lipid metabolism of L. carinii and may in part cause the reduced establishment, development and growth of the parasite in pyridoxine-deficient hosts.
authors.affiliation: Department of Biological Sciences, University of Salford.
authors.forename: M A
authors.initials: MA
authors.lastname: Beg
authors.forename: J L
authors.initials: JL
authors.lastname: Fistein
authors.forename: G A
authors.initials: GA
authors.lastname: Ingram
authors.forename: D M
authors.initials: DM
authors.lastname: Storey
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005368:Filariasis / Q000469:parasitology / Q000662:veterinary*; D005370:Filarioidea / Q000201:enzymology*; D006005:Phosphorylases / Q000378:metabolism; D012376:Rodent Diseases / Q000469:parasitology*; D016205:Sigmodontinae / Q000469:parasitology*; D026681:Vitamin B 6 Deficiency / Q000469:parasitology / Q000662:veterinary*
chemical_list: D006005:Phosphorylases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase