Biallelic expression of the IGF2 gene in human breast disease.
We examined the imprinting status of the insulin-like growth factor II gene (IGF2) in a series of 20 human breast disease samples to determine if disrupted imprinting (as evidenced by biallelic expression), was a demonstrable mechanism of altered gene expression. These samples included benign (n = 7) and malignant breast lesions (n = 13). Biallelic expression of IGF2 was detectable in 67% of benign and 60% of malignant informative breast lesions. Three informative reduction mastectomies displayed normal IGF2 imprinting. The presence of this alteration in human breast tissue is a novel finding, and may contribute to tumorigenesis, possibly by favouring an enhanced proliferative milieu, during which additional mutations could occur.
McCann
A H
AH
Department of Pathology, University College Dublin (UCD), Belfield, Ireland.
Miller
N
N
O'Meara
A
A
Pedersen
I
I
Keogh
K
K
Gorey
T
T
Dervan
P A
PA
D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D001941:Breast Diseases / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000235:genetics; D002648:Child; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005348:Fibrocystic Breast Disease / Q000235:genetics; D015870:Gene Expression; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007680:Kidney Neoplasms / Q000235:genetics; D008875:Middle Aged; D012208:Rhabdomyosarcoma / Q000235:genetics; D009396:Wilms Tumor / Q000235:genetics
D017931:DNA Primers; D007335:Insulin-Like Growth Factor II