Cytotoxicity and characteristics of mitomycin C.
In order to evaluate the cytotoxicity of mitomycin C (MMC), the effects of MMC on cell viability, cell cycle and cell surface structure were studied using Chang's cultured human conjunctival cells. A 4- or 2-min exposure to MMC at concentrations ranging from 0.002 to 0.04% decreased the cell viability. The cell number was remarkably reduced by exposure to MMC over 0.004%. MMC at a concentration higher than 0.001% arrested the DNA synthetic phase of the cell cycle. MMC even at a high concentration (0.04%) did not cause any direct alteration to the cell surface.
Murayama
T
T
Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan.
Takahashi
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N
Ikoma
N
N
D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016685:Mitomycin / Q000633:toxicity*; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity*
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D004247:DNA