Conformational dynamics of DNA during biased sinusoidal field gel electrophoresis.
DNA motion during biased sinusoidal field gel electrophoresis around the antiresonance condition was investigated by direct observation using fluorescent microscopy and Brownian dynamics simulation. Time development of the center of mass velocity, vx, and the principal value of the gyration tensor, R1, was measured at this antiresonance condition. The typical stretch-contract motion, which is observed in steady field and at high frequency field, is severely suppressed, and there are two or more dominant kinks that compete with each other. Analysis of the kink motion from the simulation results supports this picture.
Masubuchi
Y
Y
School of Engineering, Nagoya University, Japan.
Oana
H
H
Matsumoto
M
M
Doi
M
M
Yoshikawa
K
K
D017122:Bacteriophage T4 / Q000235:genetics; D003198:Computer Simulation; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013997:Time Factors
D004279:DNA, Viral; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI