Quantitative gene expression of two types of glycine transporter in the rat central nervous system.
Nuclease protection assays were performed to determine the levels of gene expression for the glycine transporters (GLY(T)), GLY(T)-1a, GLY(T)-1b and GLY(T)-2 in select regions of rat CNS. Results showed regional differences in GLY(T)-1a and GLY(T)-1b gene expression throughout the central nervous system (CNS) whereas GLY(T)-2 was predominantly expressed in the caudal brain. Although the distribution of GLY(T) correlates with the regional distribution of the glycine and N-methyl-D-aspartate receptor, areas shown to be highly susceptible to acute ischemia consistently showed low levels of GLY(T) mRNAs.
Goebel
D J
DJ
Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D017931:DNA Primers; D015870:Gene Expression; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins; D016376:Oligonucleotides, Antisense; D009928:Organ Specificity; D051381:Rats; D012160:Retina / Q000378:metabolism
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D017931:DNA Primers; D050487:Glycine Plasma Membrane Transport Proteins; D016376:Oligonucleotides, Antisense; D005998:Glycine