title: An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region.
abstract: The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high-resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites, etc. Furthermore, our results have exemplified a strategy for the completion of the chromosome 21 map to sequencing.
authors.affiliation: Institute of Physical and Chemical Research, RIKEN Gene Bank, Tsukuba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Osoegawa
authors.forename: R
authors.initials: R
authors.lastname: Susukida
authors.forename: S
authors.initials: S
authors.lastname: Okano
authors.forename: J
authors.initials: J
authors.lastname: Kudoh
authors.forename: S
authors.initials: S
authors.lastname: Minoshima
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: J
authors.initials: J
authors.lastname: Groet
authors.forename: J
authors.initials: J
authors.lastname: Ives
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: E
authors.initials: E
authors.lastname: Soeda
mesh_terms: D017101:Bacteriophage P1 / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D016386:Chromosome Walking; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004314:Down Syndrome / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008297:Male; D015183:Restriction Mapping