Isolation of toxin TsTX-VI from Tityus serrulatus scorpion venom. Effects on the release of neurotransmitters from synaptosomes.
A detailed procedure for the purification of Tityustoxin-VI, TsTX-VI, from Tityus serrulatus scorpion venom is described. For comparative purposes, a second toxin, CM-VI, obtained from the same fractionation procedure, was analyzed in parallel. Typical biochemical parameters, such as electrophoretic migration, mol.weight, amino acid composition and N-terminal sequence (first 42 amino acid residues out of a total of approx. 60) were determined for both. Our data showed that CM-VI is identical or extremely homologous to gamma-toxin (TsTX-I), the highly toxic major toxin from T. serrulatus venom. TsTX-VI was less toxic, although still effective at inducing an allergic reaction, lacrymation and contraction of the hind legs of mice. Both toxins produced a dose dependent release of the neurotransmitters glutamic acid and gamma aminobutyric acid from rat brain synaptosomes, this effect being blocked by tetrodotoxin.
Sampaio
S V
SV
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Coutinho-Netto
J
J
Arantes
E C
EC
Marangoni
S
S
Oliveira
B
B
Giglio
J R
JR
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000378:metabolism; D051379:Mice; D009498:Neurotoxins / Q000302:isolation & purification*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
C103371:CM-VI toxin; D009498:Neurotoxins; D018377:Neurotransmitter Agents; D012604:Scorpion Venoms; C068452:toxin TsTX-VI; D018698:Glutamic Acid; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid