Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein.
A full-length cDNA encoding an aspartic protease (LeAspP) has been cloned from a tomato leaf cDNA library. Using LeAspP cDNA as a probe in gel blots, LeAspP mRNA was shown to be systemically induced in tomato leaves by wounding. Application of methyl jasmonate to leaves of intact tomato plants, or supplying systemin to young tomato plants through their cut stems, induces synthesis of LeAspP mRNA. LeAspP message is regulated in tomato similar to several systemic wound response proteins (swrps) that are part of the defense response in tomato plants directed against herbivore attacks.
Schaller
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Institute of Biological Chemistry, Washington State University, Pullman 99163-6340, USA.
Ryan
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CA
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D018551:Solanum lycopersicum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D054883:Oxylipins; D010455:Peptides / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010455:Peptides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; C073704:systemin; C072239:methyl jasmonate; D016282:Aspartic Acid Endopeptidases