Cloning and characterization of the rat neurotensin receptor gene promoter.
The 5'-terminal region of the rat neurotensin receptor (NTR) gene was isolated and characterized. Genomic Southern analysis revealed that the NTR gene occurs as a single copy in the rat haploid genome. The upstream putative promoter region did not contain canonical TATA or CAAT boxes but has a consensus sequence for the transcription factor Sp1. This promoter is embedded in a large G + C-rich domain with characteristics of an CpG island. Transfection experiments using neurotensin receptor-luciferase fusion genes demonstrated that the 5'-flanking sequence functions as a strong promoter in the NG-108-15 cell. Deletion analysis suggested the presence of a core promoter (-470 to -662) that drives the minimal expression of the NTR gene.
Maeno
H
H
Department of Neuroanatomy, Biochemical Research Center, Osaka University Medical School, Japan. maeno@ncnpja.ncnp.go.jp
Yoshimura
R
R
Fujita
S
S
Su
Q
Q
Tanaka
K
K
Wada
K
K
Kiyama
H
H
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016384:Consensus Sequence; D005910:Glioma; D006238:Haploidy; D006822:Hybrid Cells; D008156:Luciferases / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D018028:Receptors, Neurotensin / Q000096:biosynthesis* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014162:Transfection
D018028:Receptors, Neurotensin; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D008156:Luciferases