[Pharmacologic modification of intestinal reperfusion injury in the animal experiment].
We evaluated experimentally (80 Lewis-rats) possible pharmacological strategies in the treatment of intestinal reperfusion injury in hypo- and normothermia. We used a specific perfusion solution containing PGI2 or radical scavengers (superoxide dismutase, oxypurinol, tocopherol, ascorbate). Decreased malondialdehyde (MDA) plasma release after reperfusion proved the antioxidative efficiency of the administered radical scavengers (normothermia-control group: MDA increase after 15 min of reperfusion to 160 +/- 30% compared to level at the end of ischemia, oxypurinol: 110 +/- 23%, tocopherol: 112 +/- 12%, ascorbate: 104 +/- 20%; p < 0.05). The ATP/ADP-ratio of the therapy groups was stable in contrast to the control group. Alkaline phosphatase release was significantly diminished under radical scavenger administration (normothermia/15 min reperfusion-control group: 7.7 +/- 0.9 mumol/ls, oxypurinol: 4.4 +/- 0.4 mumol/ls, tocopherol: 3.5 +/- 0.1 mumol/ls, ascorbate: 5.9 +/- 0.3 mumol/ls; p < 0.05). Histologically we observed a mucosa protective effect particularly in the ascorbate group. Other pharmacological strategies are discussed.
Lehmann
C
C
Klinik für Anaesthesiologie und Intensivtherapie, Humboldt-Universität zu Berlin.
Luther
B
B
Kox
W J
WJ
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage; D004734:Energy Metabolism / Q000187:drug effects; D011464:Epoprostenol / Q000008:administration & dosage*; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D007036:Hypothermia, Induced; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010117:Oxypurinol / Q000008:administration & dosage; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D013482:Superoxide Dismutase / Q000008:administration & dosage; D024505:Tocopherols; D014665:Vasodilator Agents / Q000008:administration & dosage*; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D016166:Free Radical Scavengers; D014665:Vasodilator Agents; D014810:Vitamin E; D008315:Malondialdehyde; D011464:Epoprostenol; D013482:Superoxide Dismutase; D010117:Oxypurinol; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D024505:Tocopherols