title: Protection against amebic liver abscess formation in the severe combined immunodeficient mouse by human anti-amebic antibodies.
abstract: We have used serum from patients with amebic liver abscess to investigate the role of antibody in the prevention of invasive amebiasis using the severe combined immunodeficient (SCID) mouse model of Entamoeba histolytica infection. The SCID mice were passively immunized with serum or purified antibody from patients with amebic liver abscess 24 hr prior to the direct intrahepatic challenge with 10(6) virulent E. histolytica trophozoites. This treatment reduced the mean abscess size in these animals from 24.5% to 3.5% (P < 0.0001). These results demonstrate that human anti-amebic antibodies are capable of exerting a protective effect in an animal model of amebic liver abscess formation.
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: K B
authors.initials: KB
authors.lastname: Seydel
authors.forename: K L
authors.initials: KL
authors.lastname: Braun
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: T F
authors.initials: TF
authors.lastname: Jackson
authors.forename: S L
authors.initials: SL
authors.lastname: Stanley
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D008101:Liver Abscess, Amebic / Q000517:prevention & control*; D051379:Mice; D016513:Mice, SCID
chemical_list: D000913:Antibodies, Protozoan