title: Isolation and amino acid sequence of a cystatin-type cysteine proteinase inhibitor from bovine hoof.
abstract: A low molecular mass cysteine proteinase inhibitor was found and isolated from bovine hoof. Purification of the inhibitor to homogeneity was performed by carboxymethyl-papain-sepharose affinity and DE-52 ion exchange chromatographies. From 15 g of bovine hoof, 0.25 mg of cysteine proteinase inhibitor was isolated. It had 100 amino acid residues and molecular mass of 11 406 Da, and its N-terminus was blocked. The amino acid sequence of the inhibitor demonstrated high similarity with that of the family 1 group, especially bovine thymus-derived cystatin. Thus, in summary, bovine hoof contains a cystatin-type cysteine proteinase inhibitor, presumably a family 1 cystatin.
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsushima
authors.forename: K
authors.initials: K
authors.lastname: Higashiyama
authors.forename: H
authors.initials: H
authors.lastname: Mine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015891:Cystatins / Q000737:chemistry / Q000302:isolation & purification*; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D006724:Hoof and Claw / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors