Replication-defective adenovirus type 5 as an in vitro and in vivo gene transfer vector in chickens.
The capacity of E1A gene-deleted and thus replication-defective adenovirus type 5 (Ad5) to transduce foreign genes in chicken embryo fibroblasts (CEF) as well as in chickens was investigated. The lacZ and luciferase genes were successfully transduced in CEF by replication-defective Ad5, demonstrating that these cells possess receptor(s) for binding and penetration of Ad5. A single intramuscular inoculation of Ad-gD, a replication-defective Ad5 harbouring the gD gene of pseudorabies virus, in adult and 1-day-old chickens led to the production of a very high titres of specific antibodies. These gD-specific antibodies persisted for at least 56 days. These results demonstrate that replication-defective Ad5, despite its mammalian origin and the deletion of the E1A gene, is a good candidate for developing non-spreading vaccines in poultry.
Adam
M
M
Unité de Génétique Moléculaire, INRA, Ecole Nationale Vétérinaire d'Alfort, France.
Oualikene
W
W
Le Cocq
H
H
Guittet
M
M
Eloit
M
M
D017500:Adenovirus E1A Proteins / Q000235:genetics; D000260:Adenoviruses, Human / Q000235:genetics* / Q000502:physiology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D005347:Fibroblasts; D017353:Gene Deletion; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D008156:Luciferases / Q000235:genetics; D009500:Neutralization Tests; D014614:Vaccines, Synthetic / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics*; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics
D017500:Adenovirus E1A Proteins; D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins; D008156:Luciferases; D001616:beta-Galactosidase