UVA-induced oxidative damage in fibroblasts cultured in a 3-dimensional collagen matrix.
The exposure of dermal equivalent, i.e., fibroblasts cultured in a 3-dimensional collagen matrix to realistic doses of UVA (< or = 200 kJ/m2), results in a lipid peroxidation process as evidenced by the release of thiobarbituric acid-reactive substances in the supernatant. This peroxidative process is shown to be associated with the presence of fibroblasts and is inhibited by preincubation with vitamin E, the well-known chain-breaking antioxidant. Moreover, the UVA irradiation triggers cytotoxic effects which can also be reversed by preincubation with vitamin E. While the peroxidation extent is similar for fibroblasts cultured in monolayers or in dermal equivalent, the cytotoxic response to UVA is more pronounced in dermal equivalent.
Coulomb
B
B
INSERM U.312, Laboratoire de Dermatologie, Hôpital Saint Louis, Paris, France.
Lebreton
C
C
Mathieu
N
N
Morlière
P
P
D000328:Adult; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D003094:Collagen; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D008875:Middle Aged; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology*
D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D003094:Collagen