Application of capillary electrophoresis of characterizing interactions between drugs and bile salts. Part I.
Capillary electrophoresis offers a new way of characterizing interactions between different bile salts and drugs. The observed interactions were characterized with modified model functions known from affinity capillary electrophoresis (ACE) an micellar electrokinetic capillary electrophoresis (MECC). The methodical background of both methods is the change of the ionic mobility of the drug caused by partition between phases and aggregation with the bile salt molecules, respectively. This phenomenon is described by two different physicochemical models. A parameter estimation was carried out in order to obtain the partition coefficients KP as well as constants for the aggregate formation KA. Furthermore, an expression about the specific molar volume of the micelles and stoichiometric coefficients can be given.
Schwarz
M A
MA
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther University, Halle/Saale, Germany.
Neubert
R H
RH
Rüttinger
H H
HH
D001262:Atenolol / Q000737:chemistry; D001647:Bile Acids and Salts / Q000737:chemistry*; D002701:Chloramphenicol / Q000737:chemistry; D004008:Diclofenac / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005999:Glycochenodeoxycholic Acid / Q000737:chemistry; D006000:Glycocholic Acid / Q000737:chemistry; D006002:Glycodeoxycholic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D008956:Models, Chemical; D009994:Osmolar Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011433:Propranolol / Q000737:chemistry; D011803:Quinine / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry; D013656:Taurocholic Acid / Q000737:chemistry; D013752:Tetracycline / Q000737:chemistry
D001647:Bile Acids and Salts; D008823:Micelles; D004364:Pharmaceutical Preparations; D012459:Salicylates; D004008:Diclofenac; D006002:Glycodeoxycholic Acid; D001262:Atenolol; D013656:Taurocholic Acid; D005999:Glycochenodeoxycholic Acid; D002701:Chloramphenicol; D011433:Propranolol; D011803:Quinine; D013752:Tetracycline; D006000:Glycocholic Acid