Feeding corn oil to vitamin E-deficient pigs increases lipid peroxidation and decreases tissue glutathione concentrations.
Dietetic microangiopathy ("mulberry heart disease") is a common disease of weaned pigs in several countries. It is characterised by sudden death and has been associated with vitamin E deficiency. We investigated whether it could be induced by depleting pigs of vitamin E with or without a mild peroxidative challenge. In a 2 x 2 experiment, the effect on pigs of depletion of alpha-tocopherol and supplementation with alpha-tocopherol-stripped corn oil were investigated. Although dietetic microangiopathy was not induced, there was evidence of lipid peroxidation, as judged by increased concentrations of Fe++(-)induced 4-hydroxynonenal (4-HNE) and decreased amounts of linolenic acid (C18:3, omega-3) in tissue. Reduced glutathione (GSH) can conjugate to 4-HNE in an attempt to detoxify this highly toxic compound. GSH concentrations were decreased in skeletal muscle, but not in heart, of pigs that were depleted of alpha-tocopherol with or without supplementation with corn oil. The activity of glucose-6-phosphate dehydrogenase (G6PDH) was higher in heart than in skeletal muscles. It is postulated that sufficient NADPH may be produced in heart to maintain GSH concentrations at a level sufficient to conjugate the excess 4-HNE produced by alpha-tocopherol deficiency and/or oil supplementation.
Nolan
M R
MR
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, United Kingdom.
Kennedy
D G
DG
Blanchflower
W J
WJ
Kennedy
S
S
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D003314:Corn Oil / Q000008:administration & dosage*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D015227:Lipid Peroxidation; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism / Q000662:veterinary*
D000447:Aldehydes; D004042:Dietary Fats, Unsaturated; D009249:NADP; D003314:Corn Oil; D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione; C027576:4-hydroxy-2-nonenal