The gene for human glutaredoxin (GLRX) is localized to human chromosome 5q14.
Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-dependent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH, and glutathione reductase. A cDNA encoding the human glutaredoxin gene (HGMW-approved symbol GLRX) has recently been isolated and cloned from a human fetal spleen cDNA library. The screening of a human genomic library in Charon 4A led to the identification of three genomic clones. Using fluorescence in situ hybridization to metaphase chromosomes with one genomic clone as a probe, the human glutaredoxin gene was localized to chromosomal region 5q14. This localization at chromosome 5 was in agreement with the somatic cell hybrid analysis, using DNA from a human-hamster and a human-mouse hybrid panel and using a human glutaredoxin cDNA as a probe.
Padilla
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CA
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden. bb1papec/lucano.uco.es
Bajalica
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S
Lagercrantz
J
J
Holmgren
A
A
D000818:Animals; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes / Q000235:genetics; D054477:Glutaredoxins; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics*
D018076:DNA, Complementary; C516005:GLRX protein, human; C516006:Glrx protein, mouse; D054477:Glutaredoxins; D011506:Proteins; D004247:DNA; D010088:Oxidoreductases