Over-expression of a C-terminal region of phytochrome B.
In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein of predicted size at levels higher than that of the endogenous phyB by immunoblot analysis. Although these transgenic plants did not show any phenotype in the dark, enhancement of the phyB-dependent inhibition of hypocotyl elongation and reduction of the phytochrome A (phyA)-dependent inhibition were observed.
Sakamoto
K
K
Laboratory for Photoperception and Signal Transduction, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Nagatani
A
A
D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins; D003624:Darkness; D018546:Hypocotyl / Q000528:radiation effects; D008027:Light; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D010786:Photoreceptor Cells; D010834:Phytochrome / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051942:Phytochrome A; D051939:Phytochrome B; D030821:Plants, Genetically Modified / Q000528:radiation effects; D011994:Recombinant Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D014157:Transcription Factors
D029681:Arabidopsis Proteins; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D010446:Peptide Fragments; D051942:Phytochrome A; D011994:Recombinant Proteins; D014157:Transcription Factors; D010834:Phytochrome; D051939:Phytochrome B