Effects of perfluorochemical-based artificial blood compounds in discordant xenotransplantation.
Fluosol-DA and FC43 emulsion were developed as red blood cell substitutes. However, these perfluorochemical (PFC)-based compounds are not very promising because of their immunosuppressive effects. Therefore we used these compounds as immunosuppressive agents in guinea pig-to-rat discordant heart xenotransplantations. In rats treated with these compounds (5 microliters/g body weight), rhythmic beating of the hearts was maintained for 265.7 +/- 50.3 min (Fluosol-DA-treated group) and 424.3 +/- 40.1 min (FC43 emulsion-treated group), respectively whereas the untreated heartbeat lasted for only 15.5 +/- 6.6 min. In the xenografted heart treated with PFC compounds, no pathologic changes, such as multiple coronary thromboses due to hyperacute rejection, had occurred by 3-4 h after reperfusion, and the endothelial cells were well preserved. We conclude that these PFC compounds can prolong the survival time of guinea pig heart xenografts by inhibiting thrombus formation in the xenografted heart.
Wada
S
S
First Department of Surgery, Hiroshima University, School of Medicine, Japan.
Kajihara
H
H
Murakami
H
H
Sueda
T
T
Matsuura
Y
Y
D000704:Analysis of Variance; D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage / Q000633:toxicity*; D004338:Drug Combinations; D004655:Emulsions; D005466:Fluorocarbons / Q000008:administration & dosage / Q000633:toxicity*; D006084:Graft Rejection / Q000139:chemically induced / Q000276:immunology*; D006168:Guinea Pigs; D016027:Heart Transplantation / Q000276:immunology / Q000401:mortality; D006895:Hydroxyethyl Starch Derivatives; D007165:Immunosuppression Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D014183:Transplantation, Heterologous / Q000009:adverse effects / Q000276:immunology*
D001802:Blood Substitutes; D004338:Drug Combinations; D004655:Emulsions; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C008807:perfluorotributylamine; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination