Determination of glutathione and related aminothiols in mouse tissues by gas chromatography with flame photometric detection.
A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and gamma-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted into their N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.
Takagi
K
K
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Kataoka
H
H
Makita
M
M
D000818:Animals; D002849:Chromatography, Gas; D005978:Glutathione / Q000032:analysis*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010783:Photometry / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis*
D013438:Sulfhydryl Compounds; D005978:Glutathione