Temperature modulation of calcium channel function in GH3 cells.
The effect of temperature on calcium channel function was studied in GH3 cells, using the whole cell patch-clamp methodology. Specific parameters examined were peak current amplitude, activation time, and inactivation time. Over the temperature range studied (22-37 degree C), the peak current amplitude was found to be a non-linear function of temperature, with low-threshold currents more temperature dependent than high-threshold currents. Both activation time and inactivation time were faster at higher temperatures, but, whereas inactivation was a continuous function of temperature, activation was not, with a distinct discontinuity between 27 and 32 degrees C. These findings suggest that caution must be exercised in extrapolating data on channel function obtained at room temperature to physiological temperatures.
Rosen
A D
AD
Department of Neurology, School of Medicine, State University of New York, Stony Brook 11794-8121, USA.
D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D002460:Cell Line; D018408:Patch-Clamp Techniques; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D013696:Temperature
D015220:Calcium Channels