Reversed-phase HPLC separation of proteins on chemically bonded silica gel columns.
Reversed-phase high-performance liquid chromatographic (RP-HPLC) separation of proteins on chemically bonded silica gel columns is described. Efficiency of nonporous alkylsilyl bonded silica gel is compared with that of a macroporous gel that has been widely used for the purpose. A comparative study of the separation under conventional and fast separation conditions is also given. The fast separation technique on the nonporous reversed-phase column has the advantage of improving the recovery of late-eluting hydrophobic and large proteins, such as ovalbumin and apoferritin.
Nimura
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Laboratory of Analytical Chemistry, Kitasato University, Tokyo, Japan.
Itoh
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H
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins; D058428:Silica Gel; D012822:Silicon Dioxide