The influence of CD4+ T cells, HIV disease stage and zidovudine on HIV isolation in Bahia, Brazil.
HIV-1 isolation was attempted on 72 individuals, including persons with known HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ability to detect HIV-1 from culture supernatant by p24 antigen capture assay was evaluated by segregating patients by absolute CD4+ cell counts, clinical stage of disease, p24 antigenemia and zidovudine use. The likelihood of a p24 positive HIV culture was highest among patients with CD4+ T-cell counts below 200/ul and patients with advanced clinical disease. Use of zidovudine did not affect the rate of HIV positivity in cultures.
Brites
C
C
Laboratório de Retrovírus, Hospital Universitáriò Prof. Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brazil.
Pedroso
C
C
Silva
N
N
Johnson
W D
WD
Badaró
R
R
D000998:Antiviral Agents / Q000627:therapeutic use*; D001938:Brazil; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D018709:Statistics, Nonparametric; D014776:Virus Cultivation; D015215:Zidovudine / Q000627:therapeutic use*
D000998:Antiviral Agents; D016655:HIV Core Protein p24; D015215:Zidovudine