title: Molecular epidemiologic analysis of Japanese patients with molluscum contagiosum.
abstract: BACKGROUND
  Molluscum contagiosum virus (MCV) causes molluscum contagiosum (MC) in both children and adults. Recent studies have revealed that the DNA of MCV can be classified into two major types by restriction enzyme cleavage patterns; however, the relationship between MCV types and the clinical features has not been fully understood. Our study was conducted to examine whether there are geographic differences in the incidence of MCV types and whether a correlation exists between MCV types and the age, sex, and clinical status of the patients.
  
  
  METHODS
  Specimens were obtained from 171 Japanese patients. The total DNA was extracted and digested with the restriction enzymes, BamH I, Hind III, and Cla I, respectively. Specimens were then electrophoresed in agarose gels. The gels were stained with ethidium bromide and photographs were taken under transillumination.
  
  
  RESULTS
  Six different cleavage patterns were observed; they were classified into two major types, MCV 1 and MCV 2, consisting of two MCV 1-variants, and MCV 2 prototype, and three MCV 2-variants. The ratio of MCV 1 to MCV 2 was 13:1. MCV 1 was commonly detected in children (98%) and adult women (92%). MCV 2 was more frequently isolated from adult men (44%) and from patients with human immunodeficiency virus (HIV) infection (75%).
  
  
  CONCLUSIONS
  MCV types found in Japanese children and adult women were predominantly MCV 1 and less frequently MCV 2. This pattern is similar to that observed in European countries and Australia, suggesting a high frequency and world-wide distribution of MCV 1. The higher incidence of MCV 2 among adult men and HIV-positive patients may indicate that transmission routes of MCV 1 and MCV 2 is somewhat different, of which the latter may be in part by sexual contact.
authors.affiliation: Department of Dermatology, Tokyo Women's Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Uemura
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002648:Child; D004279:DNA, Viral / Q000032:analysis / Q000145:classification*; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005260:Female; D005456:Fluorescent Dyes; D015658:HIV Infections / Q000235:genetics / Q000821:virology; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008976:Molluscum Contagiosum / Q000235:genetics* / Q000635:transmission / Q000821:virology; D008977:Molluscum contagiosum virus / Q000235:genetics*; D012737:Sex Factors; D015229:Sexually Transmitted Diseases, Viral / Q000235:genetics / Q000821:virology; D014172:Transillumination
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004996:Ethidium