In vitro proliferative advantage of bone marrow cells with tetrasomy 8 in Ewing sarcoma.
We describe a case of a 14.5-year-old boy with a clinically aggressive pelvic Ewing sarcoma. The tumor cells showed the presence of a typical t(11;22)(q24;q12) aberration and gains of chromosomes 8, 10, 14, and 21. To determine the size of the trisomy and tetrasomy 8 clones an interphase analysis by fluorescence in situ hybridization with a centromere-specific chromosome 8 probe was performed. Significant quantitative differences between metaphase and interphase data were obtained. It was shown that culturing of bone marrow cells leads to enrichment of tetrasomy 8 population that may be explained by the proliferative advantage of the tetrasomy 8 cells.
Trakhtenbrot
L
L
Institute of Hematology, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Neumann
Y
Y
Mandel
M
M
Toren
A
A
Gipsh
N
N
Rosner
E
E
Rechavi
G
G
Brok-Simoni
F
F
D000293:Adolescent; D000782:Aneuploidy; D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008297:Male; D008677:Metaphase; D012512:Sarcoma, Ewing / Q000235:genetics* / Q000473:pathology*; D013125:Spinal Neoplasms / Q000235:genetics* / Q000473:pathology*