Allelic frequencies of the HLA-B17 antigen group: comparative analysis by serology, IEF and PCR-SSOP typing.
Current typing technology for class I HLA antigens uses serological and/or isoelectric focusing gel electrophoresis. DNA typing for the HLA class I antigens can accurately identify the class I genotype of individuals and cell lines. Here, we report correlation of DNA typing results with serological and IEF results for the B17 group. The B17 antigens are relatively common, being carried by almost 9% of Caucasians and 28% of blacks. In this study, five 10th International Histocompatibility Workshop cell lines carrying B17 and 106 individuals in 61 families carrying B17 were DNA typed for B17 using B17-allele-specific amplification and sequence specific oligonucleotide probe hybridization pattern analysis. 38 (55.07%) out of 69 unrelated haplotypes had B*5701, 23 (33.33%) had B*5801, 6 (8.70%) had B*5702, and 2 (2.90%) had B*5802. DNA typing results correlated well with serological and isoelectric focusing results. In general, there was high degree of agreement between all three methods, although heterozygosity for B17 poses a particular problem for serological and IEF methodology. Both B*5701 and B*5801 have the same electrophoretic mobility on IEF gel, corresponding to B17.2, B*5702 corresponds to B17.1, while B*5802 corresponds to B17.3.
Levine
J E
JE
Immunology Program, Sloan-Kettering Institute, New York, New York, USA.
Yang
S Y
SY
D000595:Amino Acid Sequence; D001483:Base Sequence; D005787:Gene Frequency / Q000235:genetics*; D015235:HLA-B Antigens / Q000032:analysis*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007519:Isoantigens / Q000235:genetics; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012698:Serologic Tests / Q000379:methods*
D015235:HLA-B Antigens; D007519:Isoantigens; D015345:Oligonucleotide Probes