HLA typing in patients with ocular manifestations of Stevens-Johnson syndrome.
BACKGROUND
Stevens-Johnson syndrome (SJS) is an acute, self-limited, inflammatory disorder of the skin and mucous membranes. With ocular involvement, SJS has been associated with the class I human leukocyte antigen (HLA)-Bw44. This study examined HLA class II associations in patients with SJS with ocular involvement to help identify possible molecular genetic mechanisms underlying disease susceptibility/resistance.


METHODS
Twenty-three white patients with ocular complications secondary to SJS had HLA class II typing performed using polymerase chain reaction-based molecular techniques. Genotype frequency was compared with results obtained from 175 control subjects.


RESULTS
HLA-DQB1*0601 was present in four (17%) patients with SJS and in five (3%) control subjects (P < 0.05; relative risk = 7.2). There was no association with HLA-DQB1*0301, which previously has been been strongly associated with recurrent erythema multiforme. None of the class II antigens tested appeared to offer a protective effect against the development of disease.


CONCLUSIONS
HLA-DQB1*0601 was found in a significantly disproportionate number of white patients with SJS and ocular complications. The presence of this allele may confer an increased risk for the development of SJS with ocular complications and provides further evidence for an underlying immunogenetic susceptibility to the development of this disease.
Power
W J
WJ
Department of Immunology and Uveitis, Massachusetts Eye and Ear Infirmary, Boston 02146, USA.
Saidman
S L
SL
Zhang
D S
DS
Vamvakas
E C
EC
Merayo-Lloves
J M
JM
Kaufman
A H
AH
Foster
C S
CS
D000328:Adult; D000483:Alleles; D004247:DNA / Q000032:analysis; D005128:Eye Diseases / Q000209:etiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II / Q000235:genetics; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D013262:Stevens-Johnson Syndrome / Q000150:complications / Q000235:genetics*
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D004247:DNA