Polymorphism of erythrocyte acid phosphatase system (ACP, EC 3.1.3.2) in hemolysates and bloodstains. II. comparison of ACP stability in dried bloodstains in relation to the temperature of storage and the type and character of substrate.
The aim of the study was evaluation of the usefulness of selected electrophoretic methods for ACP phenotyping in simulated casework bloodstains stored at -20 degrees C, 4 degrees C, 22 degrees C and 45 degrees C. Results confirmed faster decrease of the enzyme total activity in the conditions of elevated temperature manifesting with relatively lower fluorescence and resolution of electrophoretic bands. Preliminary evaluation of 'serological history' prior to casework bloodstain analysis is suggested. Beneficial effect of lower temperature during storage until phenotyping is emphasized.
Skawrońska
M
M
Department of Forensic Medicine, Medical Academy of Białystok.
Janica
J
J
Pepiński
W
W
Nałecka
M
M
D000135:Acid Phosphatase / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D001793:Blood Preservation; D001801:Blood Stains; D004586:Electrophoresis / Q000379:methods; D004795:Enzyme Stability; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic; D013379:Substrate Specificity; D013696:Temperature
D007527:Isoenzymes; D000135:Acid Phosphatase