An electrophysiological preparation of Ascaris suum pharyngeal muscle reveals a glutamate-gated chloride channel sensitive to the avermectin analogue, milbemycin D.
An electrophysiological preparation of Ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. The preparation is shown to contain a glutamate-gated Cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin D. The application of glutamate produces a dose-dependent increase in Cl conductance and the effect of glutamate is potentiated by milbemycin D. Milbemycin D also produced a dose-dependent increase in input conductance.
Martin
R J
RJ
Department of Preclinical Veterinary Sciences, University of Edinburgh.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000900:Anti-Bacterial Agents; D017165:Ascaris suum / Q000502:physiology*; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000494:pharmacology; D004357:Drug Synergism; D004553:Electric Conductivity; D018698:Glutamic Acid / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects*; D007559:Ivermectin / Q000031:analogs & derivatives; D018942:Macrolides / Q000494:pharmacology; D008564:Membrane Potentials; D010609:Pharyngeal Muscles / Q000502:physiology; D017470:Receptors, Glutamate / Q000502:physiology
D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D018118:Chloride Channels; D002712:Chlorides; D018942:Macrolides; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D007559:Ivermectin; C027837:moxidectin