title: Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus.
abstract: The sequences of ORFs 2 to 5 of five United States (US) porcine reproductive and respiratory syndrome virus (PRRSV) isolates with differing virulence were determined. The nucleotide and deduced amino acid sequences of these isolates were compared with those of other known PRRSV isolates. The amino acid sequence identity between seven US PRRSV isolates was 91-99% in ORF 2, 86-98% in ORF 3, 92-99% in ORF 4 and 88-97% in ORF 5. The low virulence US isolate had highest sequence variation in ORFs 2 to 4 compared to the other US isolates. A hypervariable region with antigenic potential was identified within the major envelope glycoprotein. Phylogenetic analysis of ORFs 2 to 7 indicated the existence of at least three minor genotypes within the major US genotype. The low virulence US isolate formed a branch distinct from the other US isolates. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.
authors.affiliation: Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: X J
authors.initials: XJ
authors.lastname: Meng
authors.forename: P S
authors.initials: PS
authors.lastname: Paul
authors.forename: P G
authors.initials: PG
authors.lastname: Halbur
authors.forename: I
authors.initials: I
authors.lastname: Morozov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018018:Arterivirus / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000821:virology*; D014481:United States; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins