title: Cytokeratin, lectin, and acidic mucin modulation in differentiating colonic epithelial cells of mice after feeding Western-style diets.
abstract: Several studies have recently reported the development of colonic epithelial cell hyperproliferation in rodents following the ingestion of Western-style diets. In this study, additional measurements related to differentiation and maturation of the colonic epithelial cells were made after feeding this type of diet. Two Western-style diets high in fat and phosphate content and low in calcium and vitamin D were fed to C57BL/6J mice for 12, 24, and 52 weeks. Diet A contained American Blend fat as a source of lipids, diet B contained corn oil, and control diet C was a standard AIN-76A semisynthetic diet which is lower in fat content and higher in calcium and vitamin D. Colonic epithelial cells were studied for three biomarkers: cytokeratin catalogue no. 18 (clone LE64) expression, soybean agglutinin carbohydrate lectin binding, and acidic mucins including sialo- and sulfomucins. Feeding of diets A and B revealed that colonic epithelial cells had increased expression of cytokeratin catalogue 18 and SBA carbohydrate lectin binding compared to controls (P = 0.0001 for diet A versus C and diet B versus C). Significant differences were found between diets B and C (P = 0.0001) and diets A and C (P = 0.0001) in total acidic mucins and in the ratio of sialomucin:sulfomucin (P = 0.0001). These findings demonstrate that both functional and structural modifications occurred in colonic epithelial cells under these dietary conditions, and further defined this rodent model for preclinical evaluation of nutritional and chemopreventive interventions.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Fan
authors.forename: H
authors.initials: H
authors.lastname: Newmark
authors.forename: D
authors.initials: D
authors.lastname: Leung
authors.forename: M
authors.initials: M
authors.lastname: Lipkin
authors.forename: V E
authors.initials: VE
authors.lastname: Steele
authors.forename: G J
authors.initials: GJ
authors.lastname: Kelloff
mesh_terms: D000818:Animals; D002136:Calcium, Dietary / Q000008:administration & dosage*; D002454:Cell Differentiation; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D004041:Dietary Fats / Q000009:adverse effects*; D007633:Keratins / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009077:Mucins / Q000378:metabolism*; D014807:Vitamin D / Q000008:administration & dosage*
chemical_list: D002136:Calcium, Dietary; D004041:Dietary Fats; D037102:Lectins; D009077:Mucins; D014807:Vitamin D; D007633:Keratins