The effect of total saponins from Panax Ginseng C.A. Meyer on the intracellular signalling system in Ehrlich ascites tumor cells.
The mechanisms of action of total saponins from Panax Ginseng C.A. Meyer on the elements of intracellular signalling system in Ehrlich ascites tumor cells were studied. The action of total saponins was compared with the effect of ATP, a classical activator of these cells. Saponins at concentrations of 10(-6)-10(-3)% increased [Ca2+]i, mobilized Ca2+ ions from the endoplasmic reticulum (ER) and activated the influx of Ca2+ to cells. Like ATP, saponins activated the Na+/H+ exchange and Ca(2+)-dependent K+ channels. Of all the parameters, only the activation of Ca2+ influx in cell is directly affected by saponins. The changes in other parameters are connected with nonspecific activation of purinoreceptors. The analysis of the kinetic data suggests that, as distinct from ATP-dependent activation of purinoreceptor, saponins first activate the Ca2+ influx to cells and only then induce the mobilization of Ca2+ from ER.
Abdrasilov
B S
BS
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino Moscow region, Russia.
Kim YuA
Nurieva
R I
RI
Dedkova
E N
EN
Leonteva
G A
GA
Park
H J
HJ
Zinchenko
V P
VP
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D004721:Endoplasmic Reticulum / Q000187:drug effects; D051379:Mice; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D058913:Purinergic Agonists; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002120:Calcium Channel Agonists; D058913:Purinergic Agonists; D012503:Saponins; D017923:Sodium-Hydrogen Exchangers; D002118:Calcium