Fluid flow rapidly activates G proteins in human endothelial cells. Involvement of G proteins in mechanochemical signal transduction.
Fluid shear stimulates endothelial cells, with the external hemodynamic forces transduced across the plasma membrane to modulate intracellular events. We report the first direct evidence that identifies specific GTP binding proteins (G proteins) activated within 1 second of flow onset, representing one of the earliest mechanochemical signal transduction events reported to date in shear-stimulated endothelium. A nonhydrolyzable GTP photoreactive analogue, azidoanilido [alpha-32P]GTP (AAGTP), allowed irreversible labeling of flow-stimulated G proteins, with two protein bands (42 kD and 31 kD) identified in human umbilical vein endothelial cells (HUVECs) subjected to laminar flow (10 dyne/cm2) in a parallel-plate flow chamber. Immunoprecipitation of labeled whole-cell lysates identified the specific G-protein subunits G q zero/alpha 11 and G alpha i3/alpha 0) as being activated by flow. Endothelial cell membrane vesicles were sheared in a cone-and-plate viscometer, with the 42-kD protein band labeled by AAGTP, but the 31-kD protein absent, indicating that the 42-kD G protein is membrane associated and activated independently of intact cytoskeletal or cytosolic components. Our results describe one of the earliest flow-induced signaling events reported in HUVECs, providing insight into the primary mechanosensing and signal transduction mechanisms.
Gudi
S R
SR
Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.
Clark
C B
CB
Frangos
J A
JA
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D015398:Signal Transduction; D013314:Stress, Mechanical
D019204:GTP-Binding Proteins