Growth retardation--an unexpected outcome from growth hormone gene therapy in normal mice with microencapsulated myoblasts.
Recently, we have succeeded in using nonautologous myoblasts engineered to secrete mouse growth hormone (GH) to correct partially the growth retardation of the Snell dwarf mice, which suffer from pituitary GH deficiency. The allogeneic myoblasts were protected from immune rejection by enclosure in permselective microcapsules fabricated from alginate, thus validating the clinical efficacy of using universal nonautologous cells for somatic gene therapy. Because GH therapy is considered also for treating patients with normal pituitary function, we now apply this protocol to treat normal mice to evaluate the potential consequences of using GH gene therapy in subjects with no demonstrated GH deficiency. When microencapsulated allogeneic myoblasts engineered to secrete mouse GH were implanted into normal male and female mice, contrary to expectation, the treated animals became significantly shorter and lost weight; their internal organs became smaller and their tibial growth plates were less differentiated, indicating reduced skeletal growth. Females were more severely affected than males and 2 animals died by day 13 of unknown cause. By day 70, most of the abnormalities were restored to normal except for body weights, which remained below normal. In conclusion, although somatic gene therapy for GH delivery is beneficial for pituitary dwarfism, it may have adverse metabolic consequences in those with normal hypothalamic-pituitary functions, and the female mice were more severely affected than males.
Al-Hendy
A
A
Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada.
Hortelano
G
G
Tannenbaum
G S
GS
Chang
P L
PL
D000818:Animals; D002460:Cell Line; D017690:Cell Transplantation; D004339:Drug Compounding; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy / Q000009:adverse effects; D006128:Growth; D006130:Growth Disorders / Q000628:therapy; D013006:Growth Hormone / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016896:Treatment Outcome
D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone