title: Decreased aqueous-flare reaction to repeated applications of prostaglandin E2 to the cornea in pigmented rabbits.
abstract: To evaluate the effect of single or repeated topical applications of prostaglandin E2 (PGE2) to the cornea on the blood-aqueous barrier, we have measured the extent of flare in the anterior chamber of rabbit eyes at various time intervals. PGE2 was applied to the cornea for 4 min with the use of a glass cylinder in pigmented rabbits. Aqueous flare was measured by a laser flare cell meter. The flare intensity following the second application of 25 or 50 micrograms/ml of PGE2 was smaller than that following the first application. When 50 micrograms/ml of PGE2 was applied every day for 6 days, the flare intensity decreased significantly day by day. After consecutive applications of 10 micrograms/ml of PGE2 at hourly intervals, flare intensity increased up to 3 h, remained elevated from 3 to 5 h, and decreased thereafter. Repeated applications of 10 micrograms/ml of PGE2, every hour for 14 h every Monday, significantly decreased flare intensity week by week. Weekly applications of 50 micrograms/ml of PGE2 did not change flare intensity. This study indicates that the extent of the breakdown of the blood-aqueous barrier in the rabbit eye, as measured by the development of flare in the anterior chamber, is reduced with repeated PGE2 application.
authors.affiliation: Department of Ophthalmology, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Numata-Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Hiraki
authors.forename: S
authors.initials: S
authors.lastname: Hayasaka
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D018916:Blood-Aqueous Barrier / Q000187:drug effects; D002199:Capillary Permeability / Q000502:physiology; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism; D015232:Dinoprostone / Q000008:administration & dosage*; D008297:Male; D010120:Oxytocics / Q000008:administration & dosage*; D011817:Rabbits; D013618:Tachyphylaxis / Q000502:physiology*
chemical_list: D010120:Oxytocics; D015232:Dinoprostone