title: In vitro selection analysis of trans-acting HDV ribozyme.
abstract: In order to identify the functional structure as well as new active variants of the trans-acting genomic ribozyme of human hepatitis delta virus (HDV), we applied an in vitro selection procedure. After 10 generations, a randomized pool of trans-acting ribozymes accumulated in which the secondary structure of each ribozyme confirmed to the pseudoknot model and important bases in single-stranded regions were all conserved. We were surprised that mutated ribozymes derived from genomic sequence were changed to anti-genomic-like sequences. Further investigations of the most active variant confirmed that each mutated base was the most appropriate nucleotide at every position of HDV ribozyme.
authors.affiliation: National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba Science City, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Nishikawa
authors.forename: A
authors.initials: A
authors.lastname: Chiba
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: H
authors.initials: H
authors.lastname: Fauzi
authors.forename: K
authors.initials: K
authors.lastname: Taira
authors.forename: P K
authors.initials: PK
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
mesh_terms: D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D016337:RNA, Catalytic; D012367:RNA, Viral