Immunosuppressive effect of uterine fluid on spermatozoa-stimulated mixed lymphocyte cultures.
Spermatozoa, obtained from 20 healthy individuals, were incubated with crude uterine fluid or with a purified IgG fraction obtained from uterine fluid before adding them to cultures with allogeneic lymphocytes. Untreated spermatozoa induced a proliferative 1.5- to 6-fold increase, while the spermatozoa treated with crude uterine fluid or with purified IgG fraction caused 84-100% suppression of lymphocyte blastogenesis. These results suggest the existence of an endogenous uterine fluid factor, apparently IgG, which causes inhibition of lymphocyte proliferation induced by spermatozoa in the uterine lumen.
Halperin
R
R
Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin, Israel.
Peller
S
S
Schneider
D
D
Hadas
E
E
Bukovsky
I
I
Caspi
E
E
Soffer
Y
Y
D000328:Adult; D001826:Body Fluids / Q000276:immunology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000494:pharmacology; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D013094:Spermatozoa / Q000276:immunology*; D014599:Uterus / Q000276:immunology*
D007074:Immunoglobulin G