[Comparison of 4 microscopy techniques for the diagnosis of leptospirosis in wild rodents in a rural area of Valdivia, Chile].
Kidneys of six different species of 93 wild rodents captured in the rural area of Valdivia (Chile) were simultaneously examined for leptospira infection by means of Levaditi silver stain, dark ground microscopy in wet smears, and immunofluorescence and immunoperoxidase techniques using pooled antiserum against hardjo and pomona serovars. Leptospira was shown to be present in 40 (43.0%) rodents. They were detected in five of the six species: Akodon olivaceus, Akodon longipilis, Rattus rattus, Oryzomis longicaudatus and Mus musculus. Levaditi's technique detected the highest number of positive samples (67.5%) and the dark field microscopy the lowest (32.5%). The detection of leptospira in the kidneys of these wild rodents suggests that their urine may play an important role in the dissemination of leptospirosis in this area of Valdivia.
Zamora
J
J
Instituto de Microbiología, Universidad Austral de Chile, Valdivia.
Riedemann
S
S
Cabezas
X
X
Vega
S
S
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D002677:Chile / Q000453:epidemiology; D004197:Disease Reservoirs; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000382:microbiology; D007919:Leptospira / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D007922:Leptospirosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D051379:Mice / Q000382:microbiology; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence; D009115:Muridae / Q000382:microbiology*; D051381:Rats / Q000382:microbiology; D012376:Rodent Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D012423:Rural Health; D016205:Sigmodontinae / Q000382:microbiology; D016622:Silver Staining; D013045:Species Specificity