title: Interleukin 10 modulates ion transport in rat small intestine.
abstract: OBJECTIVE
  It has been proposed that the anti-inflammatory cytokine interleukin (IL)-10 might be an effective therapeutic agent in the treatment of inflammatory bowel disease. This study examined the effects of human recombinant IL-10 on ileal sodium and chloride transport in Sprague-Dawley rats.
  
  
  METHODS
  Unidirectional fluxes of sodium and chloride and tissue electrical parameters were measured under voltage-clamped conditions in ussing chambers. Intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP) were measured in isolated enterocytes.
  
  
  RESULTS
  Jejunal and ileal tissue responded to serosal addition of IL-10 with a transient decrease in short-circuit current reflecting an IL-10-induced increase in net sodium and chloride absorption because of an increase in mucosal to serosal ion movement. The IL-10-induced absorption was not prevented by tetrodotoxin but did show tachyphylaxis. IL-10 reversed, or markedly attenuated, forskolin- and carbachol-induced net chloride secretion. The effects of IL-10 on net secretion were accompanied by a reduction in forskolin-stimulated cAMP levels and a decrease in basal cAMP levels. An additional effect of IL-10 was its induction of bicarbonate secretion only in the presence of secretagogues.
  
  
  CONCLUSIONS
  This study shows that IL-10 enhances intestinal electroneutral sodium and chloride absorption, inhibits stimulated chloride secretion, and under some secretory conditions stimulates bicarbonate secretion.
authors.affiliation: Division of Gastroenterology, University of Alberta, Edmonton, Canada.
authors.forename: K L
authors.initials: KL
authors.lastname: Madsen
authors.forename: M M
authors.initials: MM
authors.lastname: Tavernini
authors.forename: T R
authors.initials: TR
authors.lastname: Mosmann
authors.forename: R N
authors.initials: RN
authors.lastname: Fedorak
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism*; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism
chemical_list: D002712:Chlorides; D016753:Interleukin-10; D005576:Colforsin; D002217:Carbachol; D012964:Sodium; D000242:Cyclic AMP