title: Relationship between outer membrane protein and lipopolysaccharide profiles and serotypes of enterotoxigenic Escherichia coli isolated in Brazil.
abstract: The electrophoretic profiles of outer membrane proteins and lipopolysaccharide of sixty-five enterotoxigenic Escherichia coli of different serotypes and virulence-associated factors, toxin and colonization factors were determined. A close relationship between serotype and outer membrane protein and lipopolysaccharide patterns could be observed. No correlation could be found between the electrophoretic profiles and the expression of virulence-associated factors. The observed homogeneity of outer membrane protein and lipopolysaccharide profiles suggested the presence of only a few clones in the samples studied, and supported the use of outer membrane protein and lipopolysaccharide analysis as a useful epidemiological tool in the characterization of enterotoxigenic Escherichia coli isolates.
authors.affiliation: Departamento de Microbiologia, Imunologia y Parasitologia, Universidade Federal de São Paulo Escola Paulista de Medicina, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Nishimura
authors.forename: L C
authors.initials: LC
authors.lastname: Ferreira
authors.forename: A B
authors.initials: AB
authors.lastname: Pacheco
authors.forename: B E
authors.initials: BE
authors.lastname: Guth
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001427:Bacterial Toxins / Q000096:biosynthesis; D001938:Brazil / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis; D017720:Molecular Epidemiology; D010641:Phenotype; D012703:Serotyping; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D008070:Lipopolysaccharides