Polymerase chain reaction for the identification of Bordetella pertussis and Bordetella parapertussis.
The polymerase chain reaction (PCR) for the detection of Bordetella pertussis and Bordetella parapertussis DNA in clinical samples was well documented by recent studies. Different regions in Bordetella pertussis DNA have been successfully used as targets for this method by various authors. In this work we report the usefulness of the PCR assay also for speciating Bordetellae isolates in those cases where the biochemical and serological tests gave inconclusive results.
Stefanelli
P
P
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Roma, Italy.
Giuliano
M
M
Bottone
M
M
Spigaglia
P
P
Mastrantonio
P
P
D000371:Agglutination; D000907:Antibodies, Bacterial / Q000032:analysis; D001884:Bordetella / Q000276:immunology / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000175:diagnosis*; D001886:Bordetella pertussis / Q000276:immunology / Q000302:isolation & purification*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D017931:DNA Primers