Regulation of leadzyme (lead-dependent ribozyme) reaction by amino acids and rare earth ions.
Effects of rare earth ions and amino acids on a novel cleavage reaction of 10mer leadzyme (lead-dependent ribozyme) CUGGGAGUCC have been investigated. Cleavage yield of RNA substrate GGACC decreases GAGCCAG by the leadzyme was 7.4% for 20 min at 25 degrees C. The cleavage reaction was performed at 5 microM leadzyme, 250 nM substrate, 25 microM Pb2+, pH 7.5. Under the same reaction condition, the addition of 25 microM Nd3+ led to the increase of the yield from 7.4% to 39.8 %, although other divalent metal ions such as Mn2+ and Co2+ showed no effect on the yield. At 75 microM Pb2+, the yield decreased from 48.0 % to 15.0 % by the addition of 10 mM Arg, although the addition of 10 mM Lys did not affect the yield. These results indicate that the cleavage reaction by the leadzyme is regulated by the combination of metal ions and amino acids.
Ohmichi
T
T
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Sasaki
M
M
Sugimoto
N
N
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D007700:Kinetics; D007854:Lead / Q000378:metabolism* / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism; D008674:Metals, Rare Earth / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D013768:Tetrahymena / Q000201:enzymology
D000596:Amino Acids; D008674:Metals, Rare Earth; D016337:RNA, Catalytic; D007854:Lead; D008274:Magnesium