Adhesive properties of P-like fimbriae in Klebsiella-species.
Clinical isolates of three encapsulated Klebsiella strains with type 1 (mannose-sensitive, MS+MR-), type 3 (mannose-resistant, MS-MR+), type 1.3 (MS+MR+) fimbriae and facultatively coexpressing P-like fimbria were investigated for their ability to adhere to uroepithelial cells (UECs) and tracheal epithelial cells (TECs). Irrespective of the type of epithelial cells, adhesion of the MS+MR+ (type 1.3) fimbriated Klebsiella strain was significantly stronger than adhesion of strains carrying only type 1 (MS+MR-) or type 3 (MS-MR+) fimbriae. The coexpression of P-like fimbriae increased the adhesive properties of Klebsiella strains to UECs but not to TECs. Adhesion of P-like fimbriated Klebsiella strains to UECs was significantly inhibited in the presence of the P+ fimbriae-specific Gal alpha-4-Gal beta (galabiose). Such adhesion was unrelated to the coexpression of type 1, type 3 or type 1.3 fimbriae. However, adhesion to TECs was only moderately inhibited.
Przondo-Mordarska
A
A
Department of Microbiology, University of Medicine, Wroclaw, Poland.
Smutnicka
D
D
Ko
H L
HL
Beuth
J
J
Pulverer
G
G
D001422:Bacterial Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004187:Disaccharides / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000378:metabolism*
D004187:Disaccharides; C032103:4-O-alpha-D-galactopyranosyl-D-galactose