Sensitive detection of near-infrared fluorescent dyes using high-performance liquid chromatography with peroxyoxalate chemiluminescence detection system.
The highly sensitive detection of four near-infrared (near-IR) fluorescent dyes, methylene blue, pyridine 1, oxazine 1 and 3,3'-diethylthiadicarbocyanine iodide (DTDCI) by high-performance liquid chromatography (HPLC) with post-column peroxyoxalate chemiluminescence (PO-CL) detection utilizing bis(4-nitro-2-(3, 6,9-trioxadecyloxycarbonyl)phenyl) oxalate (TDPO) and hydrogen peroxide was examined. The detection limits for methylene blue, pyridine 1, oxazine 1 and DTDCI were 120, 27, 31, 0.19 fmol on-column at a signal-to-noise ratio of 2, respectively. The sensitivity for DTDCI was 250 times that obtained by HPLC with the conventional fluorescent detection.
Kimoto
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Gohda
R
R
Murayama
K
K
Santa
T
T
Fukushima
T
T
Homma
H
H
Imai
K
K
D002851:Chromatography, High Pressure Liquid; D004225:Dithiazanine / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D007259:Infrared Rays; D008163:Luminescent Measurements; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D010070:Oxalates / Q000737:chemistry; D011726:Pyridinium Compounds / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity
C103429:3-(4-(4-(dimethylamino)phenyl)-1,3-butadienyl)-N-ethylpyridinium; D005456:Fluorescent Dyes; D010070:Oxalates; D011726:Pyridinium Compounds; C044622:peroxyoxalate; C103428:3,7-bis(diethylamino)phenothiazin-5-ium; D004225:Dithiazanine; D008751:Methylene Blue