Micronuclei induction by carboplatin in human lymphocyte subpopulations.
Micronuclei (MN) induction by carboplatin, cis-diammine-1,1-cyclobutane decarboxylate platinum (II) (CBDCA), in B and T lymphocytes was studied by the MAC (morphology/antibody/chromosome) method which allows the immunologic identification of different cell lineages. An increased frequency of MN in B and T lymphocytes in CBDCA-treated cultures compared with controls was observed (p < 0.001). CBD cells were found to be more sensitive to CBDCA damage. CBDCA-treated cultures showed a decrease, albeit statistically non-significant, in the proportion of CBD interphasic and mitotic cells. Furthermore, higher MN frequencies in isolated lymphocytes than in whole blood in both control and CBDCA-treated cultures were observed.
Slavutsky
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Departamento de Genética, Academia Nacional de Medicina, Buenos Aires, Argentina.
Campos
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Cid
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Larripa
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D000328:Adult; D000970:Antineoplastic Agents / Q000633:toxicity*; D001402:B-Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D016190:Carboplatin / Q000633:toxicity*; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000648:ultrastructure; D015162:Micronucleus Tests; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000648:ultrastructure
D000970:Antineoplastic Agents; D016190:Carboplatin