title: Growth hormone upregulates gastrin and peptide YY gene expression.
abstract: The purpose of these studies was to examine the effects of excess growth hormone (GH) on gastrin and peptide YY (PYY) gene expression. Transgenic mice with the bovine GH gene linked to a mouse metallothionein I promoter were used as a model of chronic GH excess. Antral gastrin mRNA and peptide levels were elevated significantly (P < 0.05) in GH transgenic mice compared with wild type littermates. Ileal PYY mRNA and ileal and colonic PYY levels were significantly elevated in GH transgenic mice compared with wild type littermates. The elevations in gastrin and PYY gene expression in GH transgenic mice were independent of food intake. Serum concentrations of gastrin and PYY were also elevated in GH transgenic mice. Immunohistochemical analysis showed that the density of PYY-containing cells in the colon of GH transgenic mice and wild type littermates did not differ. In addition, the mRNA and protein levels of chromogranin A, a marker of endocrine cells, were not increased in the colon of GH transgenic mice. Together, these data indicate that GH, insulin-like growth factor I, or both can upregulate gastrointestinal gastrin and PYY gene expression directly.
authors.affiliation: Department of Surgery, University of Texas Medical Branch, Galveston 77555, USA.
authors.forename: G
authors.initials: G
authors.lastname: Gomez
authors.forename: V
authors.initials: V
authors.lastname: Udupi
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.forename: F
authors.initials: F
authors.lastname: Lluis
authors.forename: S
authors.initials: S
authors.lastname: Rajaraman
authors.forename: J C
authors.initials: JC
authors.lastname: Thompson
authors.forename: G H
authors.initials: GH
authors.lastname: Greeley
mesh_terms: D000818:Animals; D002417:Cattle; D005755:Gastrins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D010452:Peptide Biosynthesis; D019894:Peptide YY; D010455:Peptides / Q000235:genetics
chemical_list: D005755:Gastrins; D010455:Peptides; D019894:Peptide YY; D013006:Growth Hormone