title: Arboviruses associated with mosquitoes from nine Florida counties during 1993.
abstract: Mosquitoes were collected for virus isolation tests from 36 sites in Bradford, Lake, Leon, Manatee, Orange, Osceola, Pasco, Putnam, and Sarasota counties, FL, from April 6 through October 11, 1993. A total of 158,129 adult specimens were collected in 726 trap nights using CDC light traps, usually baited with dry ice. At least 35 species were represented, although 60% of the collections was made up of 3 species (Aedes infirmatus, 6.5%; Anopheles crucians, 14.4%; and Culex nigripalpus, 39.4%). Four of the 36 collecting sites were located at waste-tire sites, where 254 trap nights yielded 27,455 specimens (17.4% of 9-county total). Forty-three virus strains were isolated from 2,812 mosquito pools consisting of 158,129 specimens. The viruses isolated include eastern equine encephalitis (EEE), 5 strains; Everglades (EVE), 2 strains; Keystone (KEY), 6 strains; Tensaw (TEN), 21 strains; trivittatus (TVT), one strain; Shark River (SR), one strain; and Flanders (FLA), one strain. In addition, 2 strains that are either KEY or Jamestown Canyon (JC) virus, and 4 ungrouped viruses remain to be identified. Twenty-one (48.8%) of the 43 virus strains were isolated from mosquitoes collected at waste-tire sites as follows: EEE (1), KEY (5), KEY/JC (1), TEN (13), and one ungrouped virus. The vector relations of the viruses are discussed and the potential importance of waste-tire sites as breeding habitats and harborages for vector and nuisance species is emphasized.
authors.affiliation: Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Mitchell
authors.forename: C D
authors.initials: CD
authors.lastname: Morris
authors.forename: G C
authors.initials: GC
authors.lastname: Smith
authors.forename: N
authors.initials: N
authors.lastname: Karabatsos
authors.forename: D
authors.initials: D
authors.lastname: Vanlandingham
authors.forename: E
authors.initials: E
authors.lastname: Cody
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D000852:Anopheles / Q000821:virology; D001103:Arboviruses / Q000302:isolation & purification*; D002522:Chlorocebus aethiops; D003465:Culex / Q000821:virology; D009033:Culicidae / Q000821:virology*; D005260:Female; D005431:Florida; D008297:Male; D014709:Vero Cells