Identification of the Fas antigen in human gingiva.
The Fas antigen is a cell-surface glycoprotein that mediates apoptosis from the cell surface into the cytoplasm. Polyclonal antibody (Fas D) was raised against a synthetic polypeptide selected from the extracellular part of the human Fas antigen (amino acid residues 104-114) and was used to detect the Fas antigen in human gingiva. Biopsy specimens of human gingiva were prepared, and the paraffin sections were reacted with the Fas D antibody by an immunohistochemical method. The antibody localized to the prickle-cell layer and to granular layer keratinocytes of human gingiva. Proteins were also prepared from human gingiva and subjected to SDS-PAGE, followed by Western-blotting analysis with the Fas D antibody. The antibody interacted with a band corresponding to an estimated molecular weight of 35 kDa. The incidence of the immunoreactive 35-kDa protein was detected in the gingiva of 90% of the 20 individuals examined. The Fas antigen detected in human gingiva may be related to the physiological turnover of oral mucosa.
Yoshioka
C
C
First Department of Oral Surgery, Kyushu Dental College, Kitakyusyu, Japan.
Muraki
Y
Y
Fukuda
J
J
Haneji
T
T
Kobayashi
N
N
D000595:Amino Acid Sequence; D017209:Apoptosis; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005881:Gingiva / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009061:Mouth Mucosa / Q000276:immunology*; D019014:fas Receptor / Q000032:analysis*
D019014:fas Receptor