Effects of Freund's complete adjuvant on the diurnal rhythms of neuroendocrine processes and ornithine decarboxylase activity in various tissues of male rats.
The aim of this study was to investigate the effects of Freund's complete adjuvant (FCA) on the diurnal rhythms of hormonal parameters in serum and ornithine decarboxylase (ODC) activity in various tissues of male rats. On days 1-2 after FCA, increase of ODC activity (used to evaluate the level of activation) was observed in the hypothalamus, pituitary gland, adrenal medulla, adrenal cortex, liver and lymphoid tissues, while the ODC activity in the kidney was reduced. This was accompanied by an increase in serum corticosterone. On days 3-4 after FCA, ODC activity remained elevated in the pituitary gland, liver and lymphoid tissues, while the ODC activity in the testes and pancreas was reduced; kidney ODC activity returned to baseline. This was associated with increased serum levels of prolactin (Prl) and luteinizing hormone, but decreased growth hormone, testosterone and insulin. The increase in ODC activity in the thymus, as well as the reduced ODC activity in the testes and kidney, can be obtained with paraffin. Furthermore, bromocryptine microcapsules (CBLA) reduced the FCA-induced increase of ODC activity in the pituitary gland, liver and lymphoid tissues (days 3-4) but did not affect the changes in other tissues. The increase in ODC activity in the pituitary gland, liver and lymphoid tissues is specific for FCA. A role for Prl in the induction of ODC in liver and lymphoid tissues is suggested by the fact that CBLA suppresses this enhancement.
Neidhart
M
M
Department of Rheumatology, University Hospital Zurich, Switzerland.
D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D005620:Freund's Adjuvant / Q000494:pharmacology*; D013006:Growth Hormone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000201:enzymology*; D007031:Hypothalamus / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008221:Lymphoid Tissue / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010902:Pituitary Gland / Q000201:enzymology; D010913:Pituitary-Adrenal System / Q000201:enzymology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000201:enzymology; D013739:Testosterone / Q000097:blood
D013739:Testosterone; D011388:Prolactin; D013006:Growth Hormone; D005620:Freund's Adjuvant; D009955:Ornithine Decarboxylase