title: Degradation of pyrimidine ribonucleosides by Pseudomonas aeruginosa.
abstract: Pyrimidine ribonucleoside degradation in the human pathogen Pseudomonas aeruginosa ATCC 15692 was investigated. Either uracil, cytosine, 5-methylcytosine, thymine, uridine or cytidine supported P. aeruginosa growth as a nitrogen source when glucose served as the carbon source. Using thin-layer chromatographic analysis, the enzymes nucleoside hydrolase and cytosine deaminase were shown to be active in ATCC 15692. Compared to (NH4)2SO4-grown cells, nucleoside hydrolase activity in ATCC 15692 approximately doubled after growth on 5-methylcytosine as a nitrogen source while its cytosine deaminase activity increased several-fold after growth on the pyrimidine bases and ribonucleosides examined as nitrogen sources. Regulation at the level of protein synthesis by 5-methylcytosine was indicated for nucleoside hydrolase and cytosine deaminase in P. aeruginosa.
authors.affiliation: Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings 57007, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: West
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism / Q000494:pharmacology; D002455:Cell Division; D002855:Chromatography, Thin Layer; D043525:Cytosine Deaminase; D005947:Glucose / Q000378:metabolism; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012263:Ribonucleosides / Q000032:analysis / Q000378:metabolism*
chemical_list: D011743:Pyrimidines; D012263:Ribonucleosides; D009699:N-Glycosyl Hydrolases; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase; D005947:Glucose; D000645:Ammonium Sulfate