title: Effects of elevated circulating IGF-1 on the extracellular matrix in "high-growth" C57BL/6J mice.
abstract: Collagen biosynthesis was analyzed in C57BL/6J mice homozygous for the high-growth locus. Plasma levels of insulin-like growth factor-1 (IGF-1) were significantly elevated in high-growth mice at all ages studied (3 wk-6 mo); IGF-binding proteins were also elevated. Skin biopsies were obtained from mice aged 3, 6, and 9 wk under halothane anesthesia. Mice were killed at 6 mo of age. Collagen, expressed per weight of tissue, was significantly increased in all tissues from high-growth mice, as was collagen cross-linking, expressed as moles of cross-link per mole of collagen. Expression of types I and III collagen, lysyl oxidase, and lysyl hydroxylase was increased in all tissues analyzed. There was a preferential increase in type III expression relative to type I expression. Rate and extent of accumulation of collagen in granulation tissue were measured in polyvinyl alcohol sponges implanted subcutaneously; collagen accumulation was significantly greater in the high-growth mice. These results suggest that 1) elevated circulating IGF-1 may increase collagen deposition both in normal tissue as well as in granulation tissue by increasing collagen gene expression, 2) IGF-1 may increase collagen cross-linking by stimulating expression of lysyl oxidase, and 3) the preferential increase in dihydroxylated cross-links observed in high-growth mice may be due to the stimulation of lysyl hydroxylase expression by IGF-1. In summary, elevated levels of IGF-1 appear to affect collagen both quantitatively and qualitatively, primarily through their effects on gene expression of collagen and of those enzymes responsible for posttranslational modifications of collagen.
authors.affiliation: Department of Internal Medicine, School of Medicine, University of California, Davis 95616, USA.
authors.forename: K
authors.initials: K
authors.lastname: Reiser
authors.forename: P
authors.initials: P
authors.lastname: Summers
authors.forename: J F
authors.initials: JF
authors.lastname: Medrano
authors.forename: R
authors.initials: R
authors.lastname: Rucker
authors.forename: J
authors.initials: J
authors.lastname: Last
authors.forename: R
authors.initials: R
authors.lastname: McDonald
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006097:Granulation Tissue / Q000378:metabolism; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000378:metabolism
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I; D003094:Collagen