title: Determination of a new antimalarial drug, benflumetol, in blood plasma by high-performance liquid chromatography.
abstract: A rapid and selective high-performance liquid chromatographic assay for determination of a new antimalarial drug (benflumetol, BFL) is described. After extraction with hexane-diethyl ether (70:30, v/v) from plasma, BFL was analysed using a C18 Partisil 10 ODS-3 reversed-phase stainless steel column and a mobile phase of acetonitrile-0.1 M ammonium acetate (90:10, v/v) adjusted to pH 4.9 with ultraviolet detection at 335 nm. The mean recovery of BFL over a concentration range of 50-400 ng/ml was 96.8 +/- 5.2%. The within-day and day-to-day coefficients of variation were 1.8-4.0 and 1.8-4.2%, respectively. The minimum detectable concentration in plasma for BFL was 5 ng/ml with a C.V. of less than 10%. This method was found to be suitable for clinical pharmacokinetic studies.
authors.affiliation: Universiti Sains Malaysia, Minden, Penang, Malaysia.
authors.forename: S M
authors.initials: SM
authors.lastname: Mansor
authors.forename: V
authors.initials: V
authors.lastname: Navaratnam
authors.forename: N
authors.initials: N
authors.lastname: Yahaya
authors.forename: N K
authors.initials: NK
authors.lastname: Nair
authors.forename: W H
authors.initials: WH
authors.lastname: Wernsdorfer
authors.forename: P H
authors.initials: PH
authors.lastname: Degen
mesh_terms: D000328:Adult; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004983:Ethanolamines / Q000097:blood* / Q000493:pharmacokinetics; D005449:Fluorenes / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D000078102:Lumefantrine; D008297:Male; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D000078102:Lumefantrine