[Analysis of dot-blot technique for the detection of three autoantibodies (anti-Jo-1, anti-M2, anti-ribosome). Comparison with reference techniques].
A recently commercialized dot-blot (Cyto-Dot, BMD) offered a new method for the detection of three autoantibodies (Ab) anti-Jo-1, anti-M2, and anti-ribosomal protein although their only common point is the cytoplasmic localisation of their respective antigen. These Ab are detected by indirect immunofluorescence (IF) (anti-M2, anti-ribosomal protein), double immunodiffusion (ID) (anti-Jo-1) and western blotting (WB) (anti-M2). The aim of the study was to compare results obtained by the Cyto-Dot with those obtained by our reference technique. One hundred and seventy-seven sera were analysed, divided into four groups: group I (n = 15) with anti-Jo-1 Ab detected by ID, group II (n = 70) with anti-M2 Ab by WB, group III (n = 33) with anti-ribosomal protein Ab by IF (of which, 19 are precipitating by ID), group IV (control group, n = 59) with 31 sera of healthy individuals, six sera of patients with liver diseases resembling primary biliary cirrhosis and 22 with a particular serological profile. Cyto-Dot is very sensitive and specific for the detection of anti-Jo-1 Ab. Also, it represents a reliable method (sensitivity 0.99) for the screening of anti-M2 Ab and for the confirmation of an atypic immunofluorescence pattern. Equivalent to ID for the detection of anti-ribosomal protein Ab, the Cyto-Dot represents a good alternative technique. However, although this new diagnostic method represents a sensitive technique for the detection of the three auto-Ab, unfortunately, it can not be applied for large series.
Roussel
B
B
Laboratoire d'immunologie, hôpital Saint-Antoine, Paris, France.
Dubel
L
L
Kantara-Doukoure
M
M
Bernard
M
M
Johanet
C
C
D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D019084:Fluorescent Antibody Technique, Indirect; D006643:Histidine-tRNA Ligase / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D005779:Immunodiffusion; D008928:Mitochondria / Q000276:immunology*; D012015:Reference Standards; D012269:Ribosomal Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D012269:Ribosomal Proteins; D006643:Histidine-tRNA Ligase