HPr: a model protein.
Histidine-containing protein (HPr) is a central component of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS). This brief review covers recent structure-function studies on the active center of this protein: the role of the active center residues in phosphotransferase; the residues contributing to the phosphohydrolysis properties of HPr; and the contribution residues in HPr make to the pKa of the transiently phosphorylated active-site residue, His 15. As well, the potential for HPr to be used as a model protein for studying problems not directly associated with its function in the PTS is discussed.
Anderson
J W
JW
Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Canada.
D001426:Bacterial Proteins / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D008958:Models, Molecular; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D006639:Histidine; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr