Effects of dNTPs on the sister-chromatid exchange (SCE) frequency in human lymphocytes.
The effects of deoxynucloside triphosphates (dNTPs) on the frequency of sister-chromatid exchange (SCE) was studied in human peripheral lymphocytes. Treatment with each dCTP and dTTP did not change the SCE frequency; however, dGTP caused a significant dose-dependent increase in SCE frequency, whereas dATP caused a significantly decreased in SCE frequency. The SCE-increasing effect of the treatment with dGTP could be totally reversed by treatment with equal concentrations of dATP or dCTP. Treatment with a mixture of four equal concentrations of dATP, dGTP, dCTP, dTTP did not alter the SCE frequency. These results suggest that the dNTP pool imbalance was a SCE-effecting factor in human lymphocytes, and that dGTP may be mainly responsible for this effect.
Xing
W
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Department of Biology, Inner Mongolia Medical College, Hohhote, China.
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D003838:Deoxyadenine Nucleotides / Q000633:toxicity*; D003845:Deoxycytosine Nucleotides / Q000633:toxicity*; D003848:Deoxyguanine Nucleotides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects; D013942:Thymine Nucleotides / Q000633:toxicity*
D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D013942:Thymine Nucleotides; C024107:2'-deoxycytidine 5'-triphosphate; C029603:deoxyguanosine triphosphate; D001973:Bromodeoxyuridine; C024157:thymidine 5'-triphosphate