Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method: an approach to human chromosome 12 and spinocerebellar ataxia 2 (SCA2).
We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia (SCA2), which is thought to be due to a (CAG/GTC)n expansion. We have isolated several clones containing (CAG/GTC)n sequences, which include previously identified sequences that map to chromosome 12. This method could be a new PCR approach for the cloning of repeats based on their proximity to Alu sequences.
Pujana
M A
MA
Molecular Genetics Department, Cancer Research Institute (IRO), L'Hospitalet de Llobregat, Barcelona, Spain.
Volpini
V
V
Estivill
X
X
D001483:Base Sequence; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D013132:Spinocerebellar Degenerations / Q000235:genetics*