title: Properties of nicked and circular dumbbell RNA-DNA chimeric oligonucleotides.
abstract: We have designed a new type of antisense oligodeoxyribonucleotide. These oligonucleotides form two hairpin loop structures with RNA-DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem. The nicked and circular dumbbell RNA/DNA chimeric oligonucleotides are molecules, with or without free ends, that are more resistant to exonuclease attack. Of particular interest, antisense DNA is liberated by RNase H treatment of the dumbbell RNA/DNA chimeric oligonucleotides.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamakawa
authors.forename: K
authors.initials: K
authors.lastname: Hosono
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002678:Chimera; D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D000079962:RNA, Circular; D016914:Ribonuclease H
chemical_list: D004270:DNA, Circular; D016376:Oligonucleotides, Antisense; D000079962:RNA, Circular; D012313:RNA; D004247:DNA; D016914:Ribonuclease H