Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice.
Transgenic mice expressing HLA-DQA1*0301 and HLA-DQB1*0302 genes (DQ8) were produced. The transgenes were then transferred into mouse (Ab degrees) class II negative mice: the only class II molecules expressed in these animals were therefore coded by the HLA-DQ8 genes. Good expression of HLA-DQ molecules was found. Both CD4+ T cells and DQ8-specific T-cell receptor V beta expressing cells were positively selected in these mice. The HLA-DQ8 molecules expressed in these animals can present various foreign and self antigens and induce T-cell proliferation in vitro. These mice will be invaluable in future studies of the structure and function of HLA-DQ8 genes.
Cheng
S
S
Department of Immunology, Mayo Clinic/Mayo Medical School, Rochester, MN 55905, USA.
Baisch
J
J
Krco
C
C
Savarirayan
S
S
Hanson
J
J
Hodgson
K
K
Smart
M
M
David
C
C
D000818:Animals; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D000949:Histocompatibility Antigens Class II