title: Cellular content of p53 protein in rat skin after exposure to the space environment.
abstract: The effects of stress in space, microgravity and space radiation, on living organisms are still unknown. We have examined the cellular content of p53 protein, a tumor-suppressor gene product, in skin from rats by the Western blot method using a blotting-amplification system. Three groups of rats were used. The first group was kept on Earth normally and showed hardly any detectable p53 protein. The second group made a 14-day flight into space on the second Spacelab Life Sciences-2 mission (F). The last group was subjected to the same kinds of stress as the rats in the second group except for spaceflight (SC). The F and the SC rats were killed on day zero (F-0 and SC-0 groups) and day nine (F-9 and SC-9 groups) after return. F-0 rats showed marked accumulation of p53 protein, whereas SC-0 rats showed a slight decrease. F-9 and SC-9 rats showed almost the same amount of p53 protein, but F-9 rats showed a slightly higher expression. From these results, it is suggested that the accumulation of cellular p53 protein is induced in rat skin cells by exposure to the space environment.
authors.affiliation: Department of Biology, Nara Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Omatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Ohira
authors.forename: S
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D000818:Animals; D015153:Blotting, Western; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013026:Space Flight; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014893:Weightlessness / Q000009:adverse effects
chemical_list: D016159:Tumor Suppressor Protein p53