title: Overexpression of calcium/calmodulin-dependent protein kinase II in insulinoma cells by use of a retroviral vector.
abstract: The manner in which increasing intracellular calcium levels lead to insulin exocytosis is not known. Possibly the signal is mediated by activation of calcium/calmodulin-dependent protein kinase II (CaM Kin II). In this work the establishment of an insulinoma cell line stably overexpressing CaM Kinase II subtype delta 2 by an ecotropic retroviral transduction system is described.
authors.affiliation: Medizinische Klinik und Poliklinik, Berufsgenossenschaftliche Krankenanstalten Bergmannsheil, Ruhr-Universität, Bochum, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Möhlig
authors.forename: P
authors.initials: P
authors.lastname: Mayer
authors.forename: P A
authors.initials: PA
authors.lastname: Horn
authors.forename: H
authors.initials: H
authors.lastname: Schatz
authors.forename: A
authors.initials: A
authors.lastname: Pfeiffer
mesh_terms: D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis*; D002460:Cell Line; D005089:Exocytosis / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005822:Genetic Vectors / Q000502:physiology*; D015151:Immunoblotting; D007340:Insulinoma / Q000201:enzymology* / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases