title: Microdialysis of a non-NMDA receptor antagonist into the L7 dorsal horn attenuates the pressor response to static muscle contraction but not passive stretch in cats.
abstract: Mean arterial pressure (MAP) and heart rate (HR) were measured during static contraction or passive stretch of the triceps surae muscle of chloralose-anaesthetized cats. MAP and HR increased by 46 +/- 5 mmHg and 17 +/- 3 beats min-1, respectively, during a 1 min contraction. Passive stretch of the same muscle for 1 min reflexly increased MAP and HR by 40 +/- 7 mmHg and 14 +/- 3 beats min-1, respectively. Microdialysis of 2 mM 6-cyano-7-nitroquinoxaline-2,3-dione (CNX), a non-NMDA receptor antagonist, into the dorsal horn at the L7 spinal level attenuated the reflex pressor response to static contraction (2 h of dialysis: delta MAP = 23 +/- 5 mmHg, delta HR = 8 +/- 2 beats min-1). By contrast, there was no attenuation of the pressor response to passive stretch at 2 h of CNQX perfusion. However, the simultaneous microdialysis of 2 mM CNQX into the L6 and S1 levels blunted the pressor and tachycardic responses to contraction and stretch. These data show that the reflex pressor response to static muscle contraction is partly mediated by activation of non-NMDA receptors at the level of afferent fibre entry into the dorsal horn and through collateral pathways. Further, it appears that the afferent pathways within the dorsal horn for the signal transduction arising from static muscle contraction and passive stretch of the hindlimb are dissimilar.
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Hand
authors.forename: G A
authors.initials: GA
authors.lastname: Ordway
authors.forename: L B
authors.initials: LB
authors.lastname: Wilson
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000008:administration & dosage* / Q000494:pharmacology; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000008:administration & dosage* / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects; D008012:Lidocaine / Q000494:pharmacology; D008161:Lumbosacral Region; D017551:Microdialysis; D009119:Muscle Contraction / Q000187:drug effects*; D009470:Muscle Spindles / Q000378:metabolism / Q000502:physiology; D016202:N-Methylaspartate / Q000494:pharmacology; D012018:Reflex / Q000502:physiology; D013116:Spinal Cord; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D016202:N-Methylaspartate; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D008012:Lidocaine