title: A model for acute carbon monoxide poisoning in conscious rats.
abstract: These experiments were designed to establish an animal model of acute carbon monoxide (CO) poisoning in awake habituated rats. On the day before exposure, under a brief anesthesia, a Levine preparation (unilateral common carotid artery occlusion) was performed on group 1 (n = 8) and 2 (n = 28), but not on group 3 rats (n = 8). Group 1 rats were exposed to air as control. Groups 2 and 3 rats were exposed to 0.27% CO in air for 60 min [carboxyhemoglobin (HbCO) = 70%] followed by a 2-day recovery in air. The Levine preparation per se did not induce any detectable physiologic effects on group 1 rats. Identical cardiovascular and metabolic responses to CO occurred in groups 2 and 3. After the CO exposure, all group 3 rats lived for 2 days with normal neurologic index (NI). In group 2 (n = 25 post-CO), 84% of the rats showed increased NI and edema of the ipsilateral cerebral hemisphere, and 76% of the rats died 8.7 +/- 1.7 h after the CO exposure. NI correlated with the brain edema (rs = 0.748, P < 0.001) and inversely correlated with the survival time after the CO exposure (rs = -0.777, P < 0.001). We therefore may conclude that exposure of the Levine-prepared rats to 0.27% CO in air for 60 min will provide a valuable model for testing of different treatments for CO poisoning.
authors.affiliation: Department of Physiology, University of Bergen, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.forename: I
authors.initials: I
authors.lastname: Tyssebotn
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001919:Bradycardia / Q000503:physiopathology; D001929:Brain Edema; D002245:Carbon Dioxide / Q000097:blood; D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000503:physiopathology; D002423:Cause of Death; D004195:Disease Models, Animal; D006339:Heart Rate / Q000502:physiology; D008297:Male; D010100:Oxygen / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015996:Survival Rate
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen