Purification and partial characterization of a fatty acid-binding protein from the yeast, Yarrowia lipolytica.
A low-molecular-mass fatty acid-binding protein was isolated from the cytosol of the yeast Yarrowia lipolytica. Purification was achieved by a two-step procedure involving size-exclusion and cation-exchange chromatography. The isolated protein exists as a monomer of 15 kDa, is basic and has a blocked N-terminus. Internal amino acid sequencing suggests that this protein may belong to a novel class of fatty acid-binding proteins.
Dell'Angelica
E C
EC
Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Ermácora
M R
MR
Santomé
J A
JA
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018996:Myelin P2 Protein / Q000737:chemistry* / Q000302:isolation & purification; D009363:Neoplasm Proteins; D019308:Palmitic Acid / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D004718:Saccharomycetales / Q000737:chemistry*; D017421:Sequence Analysis; D014357:Trypsin / Q000378:metabolism
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005656:Fungal Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D019308:Palmitic Acid; D014357:Trypsin; D003488:Cyanogen Bromide