title: Comparison of the hydrophobic grid-membrane filter DNA probe method and the Health Protection Branch standard method for the detection of Listeria monocytogenes in foods.
abstract: The standard Health Protection Branch (HPB) method for the detection of L. monocytogenes in foods involves lengthy enrichment, selection and biochemical testing, requiring up to 8 days to complete. A hydrophobic grid-membrane filter (HGMF) method employing a digoxigenin-labelled listeriolysin O probe required 5 days to complete, and included an image-analysis system for electronic data acquisition. A total of 200 food samples encompassing 8 high-risk food groups (soft and semi-soft cheeses, packaged raw vegetables, frozen cooked shrimp, ground poultry, ground pork, ground beef, jellied meats, and pâté) were screened for the presence of L. monocytogenes by the two methods. Overall, 32 (16%) and 30 (15%) of the naturally-contaminated food samples tested positive for L. monocytogenes by the HPB and DNA methods, respectively. The DNA probe method was highly specific in discriminating L. monocytogenes from other Listeria spp. present in 50 of the samples tested. Results showed 94% sensitivity and 100% specificity between the two methods. The HGMF DNA probe method is an efficient and reliable alternative to the HPB standard method for detecting L. monocytogenes in foods.
authors.affiliation: Bureau of Microbial Hazards, Health Protection Branch Health Canada, Ottawa, ON, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Yan
authors.forename: M N
authors.initials: MN
authors.lastname: Malik
authors.forename: P I
authors.initials: PI
authors.lastname: Peterkin
authors.forename: A N
authors.initials: AN
authors.lastname: Sharpe
mesh_terms: D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005374:Filtration; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial