Expression of Shigella sonnei lipopolysaccharide in Vibrio cholerae.
Making use of a newly designed mobilizable suicide vector, the genetic determinants encoding Shigella sonnei lipopolysaccharide (LPS) were stably integrated into the chromosome of the live attenuated Vibrio cholerae vaccine strain CVD103-HgR. Expression studies showed that the production of complete S. sonnei O-polysaccharide (O-PS)-bearing LPS was limited in bivalent recombinant strains that were also proficient in the synthesis of the host-encoded Inaba O-PS. Conversely, high amounts of LPS carrying S. sonnei O-PS are produced in monovalent Inaba-deficient derivatives, even in those strains which do not co-express the compatible R1 LPS core. Thus, the non-enterobacterial V. cholerae LPS core efficiently acts as a receptor for covalent binding of S. sonnei O-PS provided that competition with the host O-PS is avoided. Expression of the R1 core interferes with cell division in recombinant V. cholerae without affecting other physiological properties of vaccine strain CVD103-HgR. Both monovalent and bivalent strains stimulated high serum-antibody titres specific for their respective O-serotype(s) when administered to rabbits. The potential of V. cholerae as an expression carrier for heterologous O-serotypes is discussed.
Viret
J F
JF
Swiss Serum and Vaccine Institute, Berne, Switzerland.
Cryz
S J
SJ
Favre
D
D
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens / Q000276:immunology; D018933:Pili, Sex; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012764:Shigella sonnei / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014734:Vibrio cholerae / Q000235:genetics* / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D019081:O Antigens; D011994:Recombinant Proteins; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins