Determination of the anticancer drug prospidin in human tissue by high-performance capillary electrophoresis using derivatization.
A high-performance capillary electrophoresis (HPCE) method is described for the quantitative determination of the anticancer drug prospidin in human tissue after its derivatization with diethyldithiocarbamic acid sodium salt (DDTC). It was found that absorption of prospidin and its derivatives on the capillary wall due to the strong positive charge in the drug molecules could be eliminated by increasing the methanol content in the run buffer up to 50% and increasing the pH value up to 11.2. While studying the conditions of the interaction between prospidin and DDTC, a molar excess of the latter of 1:9 and 1.5 h of reaction time were found to be enough for complete derivatization. Sample preparation included homogenization of freshly cut papilloma species and deproteinization by methanol addition. Detection was by ultraviolet (UV) absorption at 254 nm. Due to its speed and high performance in separation, HPCE was found to be well suited for the fast checking of drug therapy in clinical practice.
Okun
V M
VM
Laboratory of Immunochemistry, All-Russian Scientific-Research Institute of Antibiotics and Enzymes, St.-Petersburg, Russia.
Aak
O V
OV
Kozlov VYu
D000970:Antineoplastic Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D010212:Papilloma / Q000737:chemistry*; D011447:Prospidium / Q000032:analysis*; D015203:Reproducibility of Results
D000970:Antineoplastic Agents; D011447:Prospidium