Rapid determination of chloroprocaine and its major metabolite, 2-chloroaminobenzoic acid, in plasma by high-performance liquid chromatography.
A sensitive and specific high-performance liquid chromatographic method for determination of the 2-chloroprocaine, local anesthetic of ester type, and its major metabolite 2-chloroaminobenzoic acid, has been developed and validated. A single-step extraction procedure is employed followed by high-performance liquid chromatographic separation using reversed-phase column and analysis using variable length UV detection. Lidocaine was used as internal standard for 2-chloroprocaine measurement and p-aminobenzoic acid was used as internal standard for 2-chloroaminobenzoic acid analysis. The analysis of spiked plasma demonstrated good accuracy and precision of the method with limit of detection 0.1 microgram/ml for 2-chloroprocaine and 0.5 microgram/ml for 2-chloroaminobenzoic acid. The method has been used for pharmacokinetic studies in laboratory animals.
Janicki
P K
PK
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, TN 37232-2125, USA.
Johnson
R
R
Kambam
J R
JR
D000779:Anesthetics, Local / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D011343:Procaine / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000097:blood
D000779:Anesthetics, Local; D011343:Procaine; D005680:gamma-Aminobutyric Acid; C004616:chloroprocaine; C029210:2-chloro-4-aminobenzoic acid