title: Does root surface conditioning with citric acid delay healing?
abstract: Effects of topical citric acid application on tissue maturation was studied in standardized periodontal defects in 6 beagle dogs. Following elevation of facial mucoperiosteal flaps, fenestration defects, 3 mm in diameter, were made through the cortical bone and recessed 0.5 mm into the dentin of maxillary canines. 1 defect in each dog was conditioned with a saturated solution of citric acid for 3 min and then rinsed with saline. Control defects in contralateral teeth were treated with saline only for the same length of time. The defects were covered with an expanded polytetrafluoroethylene membrane and the flaps repositioned and sutured. 14 days postsurgery, healing appeared more advanced along the defect walls and floor than in the center of the defect in all instances. Histometrically, citric acid-conditioned defects exhibited a higher density of collagen fibers along the defect walls and floor and adjacent to the barrier membrane as well as more advanced resolution of the residual blood clot than the surgical controls. Differences in fibroblast density within specimen pairs were non-significant. All control defects but none of the acid-conditioned defects showed an artifactual split between the dentin walls and the granulation tissue. This study failed to support the contention that topical application of citric acid to root surfaces may delay healing following periodontal surgery.
authors.affiliation: Advanced Education Program in Periodontics, Loma Linda University School of Dentistry, CA, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Selvig
authors.forename: G C
authors.initials: GC
authors.lastname: Bogle
authors.forename: T J
authors.initials: TJ
authors.lastname: Sigurdsson
authors.forename: U M
authors.initials: UM
authors.lastname: Wikesjö
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001777:Blood Coagulation; D002452:Cell Count; D019343:Citric Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D003481:Cuspid; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D005347:Fibroblasts / Q000473:pathology; D017823:Furcation Defects / Q000473:pathology / Q000601:surgery; D006097:Granulation Tissue / Q000473:pathology; D016557:Guided Tissue Regeneration, Periodontal; D008297:Male; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; D012965:Sodium Chloride; D013524:Surgical Flaps; D013997:Time Factors; D014092:Tooth Root / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D008567:Membranes, Artificial; D019343:Citric Acid; D012965:Sodium Chloride; D011138:Polytetrafluoroethylene; D003094:Collagen