Effect of chromium salts on invertase immobilization onto carboxymethyl-cellulose-gelatine carrier system.
The carboxymethylcellulose-gelatine carrier system was investigated for invertase immobilization. Chromium (III) acetate, chromium (III) sulphate and potassium chromium (III) sulphate were used as cross-linking agents. Effect of carboxymethylcellulose-gelatine ratio and cross-linker concentration on immobilized enzyme activity were analysed. Reusability of immobilized enzyme was also investigated. Maximum immobilized enzyme activities were obtained with cross-linkers chromium (III) sulphate (0.004 mol dm-3) and potassium chromium (III) sulphate (0.001 mol dm-3) for a carrier composition of carboxymethylcellulose-gelatine ratio 0.111 (w/w) as 78%.
Emregül
E
E
Department of Chemistry, Ankara University, Turkey.
Sungur
S
S
Akbulut
U
U
D002266:Carboxymethylcellulose Sodium; D017608:Chromium Compounds / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005780:Gelatin; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D043324:beta-Fructofuranosidase
D017608:Chromium Compounds; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005780:Gelatin; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D002266:Carboxymethylcellulose Sodium