title: A discrete-time, multi-type generational inheritance branching process model of cell proliferation.
abstract: Mammalian cell populations, such as tumors, may contain subpopulations differing in parameters such as cell lifetimes, even if the populations are derived from single cells. The mode of inheritance of cell lifetimes has previously been the subject of experimental and mathematical investigation. To obtain data on cell lifetimes over more cell generations then previously available, Axelrod et al. [Cell Prolif. 26:235-249(1988)] measured the number of cells in primary colonies and secondary colonies derived form the primary colonies. The experimental results indicated large variance of cells per colony and highly significant correlations between the numbers of cells in primary and secondary colonies. To mathematically model these results we derive, for previously uninvestigated multi-type Galton-Watson branching process models, the covariance of the cell counts in the primary and secondary colonies. As a result, we are able to successfully model the data with two subpopulations having differing proliferation rates, in which the proliferation rate of a daughter cell is primarily determined by the proliferation rate of its mother. Interestingly, simulations display a trade-off between high values of variances and correlation coefficients. The values obtained from experiment are located on the boundary of the region attainable by simulation.
authors.affiliation: School of Public Health, University of Texas-Houston 77225-0334, USA. stiv@hgc9.sph.uth.tmc.edu
authors.forename: D N
authors.initials: DN
authors.lastname: Stivers
authors.forename: M
authors.initials: M
authors.lastname: Kimmel
authors.forename: D E
authors.initials: DE
authors.lastname: Axelrod
mesh_terms: D000818:Animals; D002455:Cell Division; D008322:Mammals; D008954:Models, Biological; D008957:Models, Genetic; D009369:Neoplasms / Q000473:pathology; D013995:Time