Transient increase of tenascin-C in immature hippocampus: astroglial and neuronal expression.
In the present report we describe the anatomical localization of cells expressing tenascin-C, an extracellular matrix glycoprotein, in the hippocampal complex of developing rats. We report a development-dependent down regulation of both tenascin-C protein and mRNA. The highest levels of expression of tenascin-C was observed in rat pups from embryonic day 18 to postnatal day 7. Double labelling experiments performed with a tenascin-C antibody or tenascin-C probes combined with specific markers of astrocytes (GFAP) or neurons (MAP2 and Tau) allowed us to demonstrate that tenascin-C is expressed by both immature astrocytes and neurons in immature hippocampus. The temporal and topographic distribution of cells expressing tenascin-C (in the hilus and the stratum oriens of CA3) correlate with the localization and period of migration and maturation of post-mitotic cells. In view of these data we discuss the hypothesis that tenascin-C, as a mediator of neuron-glia interactions, may contribute to the development of hippocampal cells.
Ferhat
L
L
Université René Descartes (Paris V), INSERM U29, France.
Chevassus au Louis
N
N
Jorquera
I
I
Niquet
J
J
Khrestchatisky
M
M
Ben-Ari
Y
Y
Represa
A
A
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D016922:Cellular Senescence / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D019063:Tenascin / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D019063:Tenascin