Viscosity of bovine serum albumin aqueous solutions as a function of temperature and concentration.
This paper presents the results of viscosity determinations on aqueous solutions of bovine serum albumin (BSA) at a wide range of concentrations and at temperatures ranging from 5 degrees C to 45 degrees C. On the basis of these measurements a general formula connecting the relative viscosity eta r with temperature T and concentration c of the dissolved proteins was established: [formula: see text] The quantities alpha, beta, B, D and delta E are described in the text below. A simple substantiation of the formula was also given. This relation gives immediately the Mooney approximation and allows the prediction of the values of the parameter S and a self-crowding factor K in this approximation. By applying an asymptotic form of the formula such rheological quantities as the intrinsic viscosity and Huggins coefficient were calculated.
Monkos
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K
Department of Biophysics, Silesian Medical Academy, Zabrze, Poland.
D000818:Animals; D002417:Cattle; D008433:Mathematics; D008956:Models, Chemical; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity; D014867:Water
D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine