The morphometric study of the 19-day chicken embryo skeleton (gallus domesticus).
A series of 40 chicken embryos were processed after 19 d of incubation by dissection, diaphanisation, skeleton stained with alizarin red and examined by image analysis. Six bones (femur, tibiotarsus, tarsometatarsus, humerus, ulna and carpometacarpus) and 4 parameters (perimeter, area, the largest and the smallest Feret diameter) were evaluated. The smallest Feret diameter proved unusable. The coefficients of variation for the other 3 parameters were comparable for the 6 bones. All the bones were valuable for comparing morphometric values between a control population and a population treated with a xenobiotic in order to quantify the chemical's influence on ossification.
Pageze
L
L
Departement des Sciences Biologiques et Fonctionnelles, Ecole Nationale Veterinaire, Toulouse, France.
Manesse
M
M
Navarro
M
M
Carretero
A
A
Arboix
M
M
Ruberte
J
J
Sautet
J
J
D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001846:Bone Development / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000502:physiology; D002642:Chick Embryo; D004396:Coloring Agents; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005659:Fungicides, Industrial / Q000633:toxicity*; D007091:Image Processing, Computer-Assisted; D015262:Xenobiotics / Q000633:toxicity*
D000880:Anthraquinones; D004396:Coloring Agents; D005659:Fungicides, Industrial; D015262:Xenobiotics; C010078:alizarin