title: Reducing the risk of Chlamydia trachomatis genital tract infection by evaluating the prophylactic potential of vaginally applied chemicals.
abstract: The need to provide women with a partner-independent method of prophylaxis against sexually transmitted disease (STD) against, including Chlamydia trachomatis, has led to a direct effort to develop a vaginally applied, broad-spectrum antimicrobial preparation that is both safe and effective. Using a murine model of C. trachomatis lower genital tract infection, we tested the ability of various vaginally applied chemicals to alter the course of infection in female mice challenged with infectious doses ranging from 10(3) to 10(6) inclusion forming units of an oculogenital serovar of C. trachomatis. When administered in a methylcellulose/propylene glycol-base gel 15 minutes prior to challenge, four widely used topical antimicrobials (benzalkonium chloride, chlorhexidine, nonoxynol-9, and polymyxin B) were shown to protect against infection. These results support the possibility that this type of interventional method may be a clinically relevant means of prophylaxis against sexually transmitted infection with C. trachomatis, the most common bacterial STD agent.
authors.affiliation: Department of Infectious Diseases, City of Hope National Medical Center, Duarte, California 91010, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Lyons
authors.forename: J I
authors.initials: JI
authors.lastname: Ito
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage* / Q000494:pharmacology; D001548:Benzalkonium Compounds / Q000008:administration & dosage / Q000494:pharmacology; D002690:Chlamydia Infections / Q000517:prevention & control*; D002692:Chlamydia trachomatis / Q000187:drug effects*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005831:Genital Diseases, Female / Q000517:prevention & control*; D006801:Humans; D051379:Mice; D017137:Nonoxynol / Q000008:administration & dosage / Q000494:pharmacology; D011112:Polymyxin B / Q000008:administration & dosage / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D017137:Nonoxynol; D011112:Polymyxin B; C010882:chlorhexidine gluconate; D002710:Chlorhexidine