Comparative study on reused haemodialysis membranes.
Data have shown that on the internal surface of reused haemodialysis membranes complement elements of plasma proteins, cells and cellular debris from the patient's blood are deposited. The aim of our study was to identify the effect of reuse on the haemodialysis membrane surface. Original and reused haemodialysis membranes were studied by the methods of light, transmission and scanning electron microscopy, immuno-histochemistry and X-ray spectrum element analysis. With reuse the membrane surface becomes uneven, and its continuity may break. Plasma protein and cellular elements are deposited on the damaged surface following continued reuse. Presence of IgM deposists were confirmed by immunohistochemical observation. Moreover, following reuse the X-ray spectrum analysis verified structural changes in the haemodialysis tube compared to the control material; accumulated aluminium, silicon, phosphorus, sulphur and chlorine were detected on the wall of the dialysis membrane.
Sótonyi
P
P
Department of Transplantation and Surgery, Semmelweis University of Medicine, Budapest, Hungary.
Jàray
J
J
Pàdàr
Z
Z
Woller
J
J
Füredi
S
S
Gàl
T
T
D000535:Aluminum / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002713:Chlorine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017743:Equipment Reuse; D006801:Humans; D007150:Immunohistochemistry; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000378:metabolism; D006435:Renal Dialysis / Q000592:standards*; D012825:Silicon / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013455:Sulfur / Q000378:metabolism; D013499:Surface Properties
D008567:Membranes, Artificial; D010758:Phosphorus; D002713:Chlorine; D013455:Sulfur; D002482:Cellulose; C009865:cuprammonium cellulose; D000535:Aluminum; D012825:Silicon