Interleukin 10 modulates ion transport in rat small intestine.
OBJECTIVE
It has been proposed that the anti-inflammatory cytokine interleukin (IL)-10 might be an effective therapeutic agent in the treatment of inflammatory bowel disease. This study examined the effects of human recombinant IL-10 on ileal sodium and chloride transport in Sprague-Dawley rats.


METHODS
Unidirectional fluxes of sodium and chloride and tissue electrical parameters were measured under voltage-clamped conditions in ussing chambers. Intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP) were measured in isolated enterocytes.


RESULTS
Jejunal and ileal tissue responded to serosal addition of IL-10 with a transient decrease in short-circuit current reflecting an IL-10-induced increase in net sodium and chloride absorption because of an increase in mucosal to serosal ion movement. The IL-10-induced absorption was not prevented by tetrodotoxin but did show tachyphylaxis. IL-10 reversed, or markedly attenuated, forskolin- and carbachol-induced net chloride secretion. The effects of IL-10 on net secretion were accompanied by a reduction in forskolin-stimulated cAMP levels and a decrease in basal cAMP levels. An additional effect of IL-10 was its induction of bicarbonate secretion only in the presence of secretagogues.


CONCLUSIONS
This study shows that IL-10 enhances intestinal electroneutral sodium and chloride absorption, inhibits stimulated chloride secretion, and under some secretory conditions stimulates bicarbonate secretion.
Madsen
K L
KL
Division of Gastroenterology, University of Alberta, Edmonton, Canada.
Tavernini
M M
MM
Mosmann
T R
TR
Fedorak
R N
RN
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism*; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism
D002712:Chlorides; D016753:Interleukin-10; D005576:Colforsin; D002217:Carbachol; D012964:Sodium; D000242:Cyclic AMP