title: Selective deuteration of RNA for NMR signal assignment.
abstract: For site-selective deuterium labeling of RNA, [5-2H]uridine phosphoramidite was prepared. The uridine at position 10 of a 25-mer RNA, GGACAGACUUCGGUCGGAGUACUCG, was labeled in two different manners for "positive" (U = [5-1H]U, U = [5-2H] U) and "negative"(U = [5-2H]U, U = [5-1H]U) observations. By comparison of NOESY spectra of the two labeled samples with that of the unlabeled RNA, we could unambiguously assign the H5-H6 signals of U10, and measure their NOE connectivities.
authors.affiliation: Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: K
authors.initials: K
authors.lastname: Hosono
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: H
authors.initials: H
authors.lastname: Takaku
authors.forename: G
authors.initials: G
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003903:Deuterium / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011131:Polyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D011131:Polyribonucleotides; D012313:RNA; D006859:Hydrogen; D003903:Deuterium