Automated DNA typing of ACTBP2: a comparison of allelic ladder with GS 2500 as internal marker.
A comparison was made between the size values of the alleles measured by the internal size marker of Genescan (GS 2500) and the size values measured by an allelic ladder constructed by our laboratory in the application of Genescan software for the typing of the ACTBP2 system. The measurement by GS 2500 showed a greater than 2 bp divergence (SD = 0.893) while the measurement by the allelic ladder showed only 0.1 bp divergence (SD = 0.008). Thus in genotyping of ACTBP2 by Genescan software, the method presented here of using the allelic ladder as the size marker instead of GS 2500 is easier and more reliable.
Harashima
N
N
Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Liu
C
C
Katsuyama
Y
Y
Ota
M
M
Hama
H
H
Fukushima
H
H
D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012984:Software
D005819:Genetic Markers; D004247:DNA