title: Characterization of a voltage-dependent potassium channel in squid Schwann cells reconstituted in planar lipid bilayers.
abstract: An affinity column prepared with noxiustoxin (NTx), a K+ channel blocker from the venom of the Mexican scorpion Centruroides noxius, was used to purify a functional channel from a detergent extract of Schwann cell membrane of the giant axon of the squid Loligo vulgaris. The purified protein was reconstituted as a functional unit in a planar lipid bilayer and tested with a sequence of potentials to obtain information about single-channel amplitude and kinetics. The reconstituted channel showed delayed rectifier behavior with a slope conductance of 10 pS under 5:1 asymmetric KCl concentrations and a clear tendency to open under negative potentials. The zero-current potential was +36mV, which fitted well with the Nernst equation for the CIS/TRANS K(+)-concentration ratio of 5:1. The channel also showed a strong sensitivity to tetraethylammonium and its activity was inhibited by NTx, as expected from the purification procedure. The behavior of this protein in the presence of 0.5 mM ATP (cis side) was also tested, significantly increasing current fluctuations across the membrane. In order to compare the modulation of the Schwann cell K+ channel with that of the axonal K+ channel, a purified protein from the squid axon membrane was also tested in the presence of ATP. This 10-11 pS, delayed rectifier channel from the squid giant axon (Prestipino et al., FEBS Lett. 250:570-574, 1989) was also tested in the presence of ATP and showed a similar rise in activity.
authors.affiliation: Istituto di Cibernetica e Biofisica, CNR, Genova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Noceti
authors.forename: A N
authors.initials: AN
authors.lastname: Ramírez
authors.forename: L D
authors.initials: LD
authors.lastname: Possani
authors.forename: G
authors.initials: G
authors.lastname: Prestipino
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry / Q000378:metabolism*; D049832:Decapodiformes; D004594:Electrophysiology; D015640:Ion Channel Gating / Q000502:physiology; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D010766:Phosphorylation; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012583:Schwann Cells / Q000378:metabolism*; D012604:Scorpion Venoms / Q000494:pharmacology
chemical_list: D008051:Lipid Bilayers; D015221:Potassium Channels; D012604:Scorpion Venoms; C052567:noxiustoxin