Cytotoxic action of tri-n-butyltin on dissociated rat cerebellar neurones: a flow-cytometric study.
The effects of tri-n-butyltin on cell viability and intracellular Ca2+ concentration ([CA2+]i) were examined by a flow cytometer in rat cerebellar neurones to reveal the contribution of tri-n-butyltin-induced increased in the [Ca2+]i to its cytotoxicity. Tri-n-Butyltin decreased the cell viability in association with increased [Ca2+]i at concentrations of 0.3 microM or more. Decrease or increase of the extracellular Ca2+ concentration ([Ca2+]o) respectively decreased or increased the cell viability. However, cell viability in the presence of ionomycin which increased [Ca2+]i more significantly than tri-n-butyltin was higher than that in the presence of tri-n-butyltin. Tri-n-butyltin also decreased cell viability under nominally [Ca2+]o-free conditions although the [Ca2+]i increase by tri-n-butyltin was still lower than the control [Ca2+]i under normal [Ca2+]o (2 mM). Therefore, it is unlikely that neuronal death induced by tri-n-butyltin is entirely dependent on the tri-n-butyltin-induced increase in [Ca2+]i.
Ueha
T
T
Laboratory of Cell Signaling (Pharmacology), Faculty of Integrated Arts and Sciences, Graduate School of Human and Natural Environments, University of Tokushima, Japan.
Oyama
Y
Y
Tomiyoshi
F
F
D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004996:Ethidium; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D007476:Ionophores; D014220:Trialkyltin Compounds; D014966:Xanthenes; C059715:Fluo-3; C011559:tributyltin; D015759:Ionomycin; D004996:Ethidium; D002118:Calcium