Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice.
Two randomly amplified polymorphic DNA (RAPD) markers, OPF6(2700) and OPH18(2400), tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international race IB46) of the blast fungus were identified. To derive sequence characterized amplified regions (SCARs) from OPF6(2700) and OPH18(2400), these amplified RAPD products were cloned and sequenced. Nucleotide sequence information, obtained for each end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCAR's alternate alleles were considered for the indirect selection of Pi-10. Such polymorphisms converted the linked dominant RAPD loci into codominant SCAR markers and also facilitated the indirect scoring of the blast-resistant and blast-susceptible genotypes. The development of length variant codominant SCAR markers linked to a major gene for blast resistance in rice is described. The codominant SCARs will facilitate marker-assisted selection of the Pi-10 locus in rice breeding programs and will also be useful as genetic markers for high resolution mapping of the Pi-10 region.
Naqvi
N I
NI
Department of Microbiology, Maharaja Sayajirao University, Baroda, India.
Chattoo
B B
BB
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D005658:Fungi / Q000254:growth & development; D005799:Genes, Dominant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005819:Genetic Markers