Association analysis of a polymorphism of the monoamine oxidase B gene with Parkinson's disease in a Japanese population.
The polymorphic allele of the monoamine oxidase B (MAO-B) gene detected by polymerase chain reaction (PCR) and single-stranded conformation polymorphism (SSCP) was associated with Parkinson's disease (PD) in Caucasians. We characterized this polymorphic allele, allele 1, of the MAO-B gene using direct sequencing of PCR products. A single DNA substitution (G-A), resulting gain of Mae III restriction site was detected in intron 13 of the MAO-B gene. The allele associated with PD in Caucasians was twice as frequent as in healthy Japanese, but the association of the allele of the MAO-B gene was not observed in Japanese patients with PD.
Morimoto
Y
Y
Department of Hygiene, Ehime University School of Medicine, Japan.
Murayama
N
N
Kuwano
A
A
Kondo
I
I
Yamashita
Y
Y
Mizuno
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007438:Introns; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000235:genetics*; D010300:Parkinson Disease / Q000201:enzymology* / Q000235:genetics*; D017354:Point Mutation; D011110:Polymorphism, Genetic
D017931:DNA Primers; D008995:Monoamine Oxidase