title: Proteins binding to the promoter region of the operon encoding the major outer surface proteins OspA and OspB of Borrelia burgdorferi.
abstract: The synthesis of the major outer surface proteins OspA and OspB in Borrelia burgdorferi varies among strains and during in vitro cultivation. We examined B. burgdorferi CA-11.2A for the presence of proteins that bind to the ospAB operon promoter region. Three major DNA-protein complexes were detected using a mobility shift assay; one of these complexes was due to sequence-specific binding. These proteins may be involved in the regulation of ospAB transcription and the pathogenesis of Lyme disease.
authors.affiliation: Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA.
authors.forename: N
authors.initials: N
authors.lastname: Margolis
authors.forename: D S
authors.initials: DS
authors.lastname: Samuels
mesh_terms: D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001428:Bacterial Vaccines; D001667:Binding, Competitive; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008074:Lipoproteins; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D008074:Lipoproteins; C072045:OspA protein; C077030:OspB protein, Borrelia burgdorferi