title: Glucose uptake in Kluyveromyces lactis: role of the HGT1 gene in glucose transport.
abstract: A gene for high-affinity glucose transport, HGT1, has been isolated from the lactose-assimilating yeast Kluyveromyces lactis. Disruption strains showed much-reduced uptake of glucose at low concentrations and growth was particularly affected in low-glucose medium. The HGT1 nucleotide sequence implies that it encodes a typical transmembrane protein with 12 hydrophobic domains and with 26 to 31% amino acid identity with the Hxtp family of glucose transport elements in Saccharomyces cerevisiae. Expression is constitutive (in contrast to RAG1, the major gene for low-affinity glucose uptake in K. lactis) and is controlled by several genes also known to affect expression of RAG1. These include RAG5 (which codes for the single hexokinase of K. lactis), which is required for HGT1 transcription, and RAG4, which has a negative effect. The double mutant deltahgt1deltarag1 showed further reduced glucose uptake but still grew quite well on 2% glucose and was not completely impaired even on 0.1% glucose.
authors.affiliation: Institut de Génétique et Microbiologie, Institut Curie, Centre Universitaire, Orsay, France.
authors.forename: P
authors.initials: P
authors.lastname: Billard
authors.forename: S
authors.initials: S
authors.lastname: Ménart
authors.forename: J
authors.initials: J
authors.lastname: Blaisonneau
authors.forename: M
authors.initials: M
authors.lastname: Bolotin-Fukuhara
authors.forename: H
authors.initials: H
authors.lastname: Fukuhara
authors.forename: M
authors.initials: M
authors.lastname: Wésolowski-Louvel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism*; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; C491110:OPT1 protein, S cerevisiae; D012329:RNA, Bacterial; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose