Phylogenetic relationship of silkmoths inferred from sequence data of the arylphorin gene.
A portion of the arylphorin gene from 11 silkmoth species belonging to the families Saturniidae and Bombycidae was amplified by a polymerase chain reaction. Approximately 460 bp corresponding to the third exon, the third intron, and the fourth exon were successfully amplified, sequenced, and compared. Sequences corresponding to the third and fourth exons were the most conserved, whereas sequences corresponding to the third intron were conserved only between species belonging to the same genus. We also analyzed differences of substitution rates among codon positions and between synonymous and nonsynonymous sites. Phylogenetic trees were constructed using the entire nucleotide sequences, the first and second codon positions, nonsynonymous sites, transversions, and the inferred amino acid sequences in the exons by the neighbor-joining method. These trees supported the monophyly of the families Saturniidae and Bombycidae as well as the monophyly of the genera Actias, Bombyx, Antheraea, and Samia.
Shimada
T
T
Department of Agricultural and Environmental Biology, University of Tokyo, Japan.
Kurimoto
Y
Y
Kobayashi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics; D003062:Codon; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005091:Exons; D017344:Genes, Insect; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics*; D019476:Insect Proteins; D007438:Introns; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D003062:Codon; D017931:DNA Primers; D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; C041137:storage proteins, Insecta; D004247:DNA