Pre- and postsynaptic localization of a metabotropic glutamate receptor, mGluR2, in the rat brain: an immunohistochemical study with a monoclonal antibody.
A monoclonal antibody against a metabotropic glutamate receptor, mGluR2, was produced by using a glutathione S-transferase (GST) fusion protein containing an N-terminal sequence of rat mGluR2. Intense mGluR2-like immunoreactivity (mGluR2-LI) was seen mainly in neuropil of the cerebral cortical regions, hippocampus, olfactory bulb, some diencephalic nuclei, dorsal cochlear nucleus and cerebellar cortex. In the cerebellar cortex, mGluR2-LI was seen only in Golgi cells. In Ammon's horn, mGluR2-LI was marked in the stratum lucidum of CA3 and the stratum lacunosum-moleculare of CA1-CA3, but not detected in the stratum pyramidale. The results indicate that mGluR2 is located not only presynaptically but also postsynaptically.
Neki
A
A
Department of Biological Sciences, Kyoto University, Japan.
Ohishi
H
H
Kaneko
T
T
Shigemoto
R
R
Nakanishi
S
S
Mizuno
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000276:immunology / Q000378:metabolism*; D017661:Receptors, Presynaptic / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013569:Synapses / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D018094:Receptors, Metabotropic Glutamate; D017661:Receptors, Presynaptic; D011993:Recombinant Fusion Proteins