title: Simultaneous quantitation of buspirone and its major metabolite 1-(2-pyrimidinyl)piperazine in human plasma by high-performance liquid chromatography with coulometric detection.
abstract: Buspirone is a member of the azapirone group of anxiolytic drugs and has one major metabolite, 1-(2-pyrimidinyl)piperazine (1-PP). The analyte, its metabolite and the internal standard were extracted from plasma utilizing solid-phase extraction columns. Chromatography was performed using isocratic reversed-phase high-performance liquid chromatography with coulometric end-point detection. The calibration graph was linear over the range 0-50 ng ml-1 of plasma. The lower limits of quantitation for buspirone and 1-PP were 0.5 and 2 ng ml-1, respectively, when 1 ml of plasma was extracted. The intra-assay relative standard deviations (RSD) over the range of the calibration graph varied from 4 to 12.5% for buspirone and 1-PP. The inter-assay RSD was 6.9% for 1-PP and 9.6% for buspirone. The recovery averaged 96% for buspirone and 66% for 1-PP. Plasma profiles of buspirone and 1-PP following oral dosing are presented.
authors.affiliation: Department of Psychiatry, University of Oxford, UK.
authors.forename: J
authors.initials: J
authors.lastname: Odontiadis
authors.forename: M
authors.initials: M
authors.lastname: Franklin
mesh_terms: D000328:Adult; D001711:Biotransformation; D002065:Buspirone / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012015:Reference Standards; D017366:Serotonin Receptor Agonists / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D007202:Indicators and Reagents; D017366:Serotonin Receptor Agonists; C037921:1-(2-pyrimidinyl)piperazine; D002065:Buspirone