Determination of a new antimalarial drug, benflumetol, in blood plasma by high-performance liquid chromatography.
A rapid and selective high-performance liquid chromatographic assay for determination of a new antimalarial drug (benflumetol, BFL) is described. After extraction with hexane-diethyl ether (70:30, v/v) from plasma, BFL was analysed using a C18 Partisil 10 ODS-3 reversed-phase stainless steel column and a mobile phase of acetonitrile-0.1 M ammonium acetate (90:10, v/v) adjusted to pH 4.9 with ultraviolet detection at 335 nm. The mean recovery of BFL over a concentration range of 50-400 ng/ml was 96.8 +/- 5.2%. The within-day and day-to-day coefficients of variation were 1.8-4.0 and 1.8-4.2%, respectively. The minimum detectable concentration in plasma for BFL was 5 ng/ml with a C.V. of less than 10%. This method was found to be suitable for clinical pharmacokinetic studies.
Mansor
S M
SM
Universiti Sains Malaysia, Minden, Penang, Malaysia.
Navaratnam
V
V
Yahaya
N
N
Nair
N K
NK
Wernsdorfer
W H
WH
Degen
P H
PH
D000328:Adult; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004983:Ethanolamines / Q000097:blood* / Q000493:pharmacokinetics; D005449:Fluorenes / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D000078102:Lumefantrine; D008297:Male; D013056:Spectrophotometry, Ultraviolet
D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D000078102:Lumefantrine