title: Analysis of cellular receptors for human coronavirus OC43.
abstract: Bovine coronavirus (BCV), human coronavirus OC43 (HCV-OC43) and hemagglutinating encephalomyelitis virus (HEV) are serologically related viruses that all have hemagglutinating activity. The receptor determinant for attachment to erythrocytes has been shown to be N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2). We compared the ability of the three coronaviruses to recognize 9-O-acetylated sialic acid and found that they all bind to Neu5,9Ac2 attached to galactose in either A2,3 or A2,6-linkage. There are, however, some differences in the minimum amount of sialic acid that is required on the cell surface for agglutination by these viruses. Evidence is presented that HCV-OC43 uses Neu5,9Ac2 as a receptor determinant not only for agglutination of erythrocytes but also for attachment to and infection of a cultured cell line, MDCK I cells.
authors.affiliation: Institut für Virologie, Philipps-Universität Marburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Krempl
authors.forename: B
authors.initials: B
authors.lastname: Schultze
authors.forename: G
authors.initials: G
authors.lastname: Herrler
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D002460:Cell Line; D017934:Coronavirus / Q000502:physiology*; D028962:Coronavirus OC43, Human; D017938:Coronavirus, Bovine / Q000502:physiology; D004912:Erythrocytes / Q000276:immunology; D006384:Hemagglutination; D006801:Humans; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000737:chemistry / Q000502:physiology*; D012794:Sialic Acids / Q000097:blood*
chemical_list: D011991:Receptors, Virus; D012794:Sialic Acids; C025676:9-O-acetyl-N-acetylneuraminic acid