title: Biomaterial supports for colonic wall defect healing. An experimental study in the rat.
abstract: New artificial biomaterials were tested for support of gastro-intestinal tract wound healing in the rat. Double layered collagenic matrices were prepared with purified collagens extracted from human placental tissues. Two types of patches were tested, the first constituted from a collagen type I + III layer covered by collagen IV in a liquid phase (patch I + III/IV) and the second from a collagen IV layer covered by liquid collagen IV (patch IV/IV). The matrices were applied with fibrin sealant to the edges of a 1 cm diameter colonic wall defect in the rat. Healing evolution was determined by macroscopic, microscopic and immunostaining studies. The reconstitution of the three colonic wall layers was achieved within 45 days without retraction or inflammatory reaction, while the biomaterial was resorbed. Human collagen I and III antibodies failed to stain extracellular matrix. This failure may be a consequence of outdated antibodies or more likely epitope alteration during extraction and preparation of the collagens. A human collagen type IV antibody staining of the scar zone showed the basement membranes of newly developed vessels within 10 days, and newly formed colonic mucosa within 20 days. The collagen reconstituted matrix was able to assist healing of normal digestive tract defects as shown by the labelling of the new synthesized extracellular matrix by collagen type IV antibody. These findings support the use of collagen biomaterial in gastro-intestinal anastomosis. This new surgical approach allowing healing of colonic wall defects could reduce occurrence of anastomotic leakage in human.
authors.affiliation: IRCAD, Hôpital Civil, Strasbourg, France.
authors.forename: D
authors.initials: D
authors.lastname: Mutter
authors.forename: M
authors.initials: M
authors.lastname: Aprahamian
authors.forename: C
authors.initials: C
authors.lastname: Damge
authors.forename: P
authors.initials: P
authors.lastname: Sonzini
authors.forename: J
authors.initials: J
authors.lastname: Marescaux
mesh_terms: D000818:Animals; D000906:Antibodies; D001672:Biocompatible Materials; D003094:Collagen; D003106:Colon / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D006801:Humans; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D014945:Wound Healing
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials; D003094:Collagen