Identification of an intragenic interferon-stimulated response element sequence of the mouse class I major histocompatibility complex H-2Kb gene.
A 5'-enhancer-lacking construct of the mouse MHC class I H-2Kb gene is stimulated by interferons in a similar manner as a 5'-enhancer-containing H-2Kb gene. This ability is markedly reduced upon introducing a second deletion removing the first and second intron of the H-2Kb gene. Computational analysis of the deleted stretch reveals the presence of the interferon-stimulated response element (ISRE) in the middle of the second intron. The identified sequence exhibits typical ISRE characteristics in gel retardation experiments. A fragment containing the 5'-enhancer interferon responsive sequence competes effectively in the binding reactions. These results strongly suggest that the second intron of the H-2Kb gene participates in interferon stimulation via the identified ISRE sequence.
Hatina
V J
VJ
Laboratory of Mammalian Molecular Genetics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Králová
J
J
Jansa
P
P
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D006183:H-2 Antigens / Q000187:drug effects*; D007370:Interferon Type I / Q000494:pharmacology*; D007739:L Cells; D051379:Mice; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000235:genetics*
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D007370:Interferon Type I