title: Nuclear microanalysis of the monovalent ion distribution in the human amnion. I. Effect of magnesium.
abstract: The effect of the addition of MgCl2 on the Na+, K+, and Cl- concentration and distribution in epithelial and compact layers of the human amniotic membrane was investigated using the Bordeaux nuclear microprobe. Particle-induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological medium (Hanks' solution), the monovalent ion concentrations were identical in both layers. The addition of Mg2+ ions in Hanks' solution induced a decrease of, K+, and Cl- concentration in both layers and, Na+ concentration in the compact layer. The results obtained from nuclear microanalysis might be explained from electrophysiological data which indicate that the addition of Mg2+ ions results in an increase in the cellular, paracellular and exchanger ion pathways.
authors.affiliation: Laboratoire de Physiopathologie du Développement, Université P.M. Curie, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Bara
authors.forename: A
authors.initials: A
authors.lastname: Guiet-Bara
authors.forename: P
authors.initials: P
authors.lastname: Moretto
authors.forename: L
authors.initials: L
authors.lastname: Razafindrabe
authors.forename: Y
authors.initials: Y
authors.lastname: Llabador
authors.forename: M
authors.initials: M
authors.lastname: Simonoff
authors.forename: J
authors.initials: J
authors.lastname: Durlach
mesh_terms: D000650:Amnion / Q000737:chemistry* / Q000187:drug effects; D002712:Chlorides / Q000032:analysis; D004577:Electron Probe Microanalysis; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008274:Magnesium / Q000737:chemistry*; D008832:Microchemistry; D011188:Potassium / Q000032:analysis; D011247:Pregnancy; D012542:Scattering, Radiation; D012964:Sodium / Q000032:analysis; D014965:X-Rays
chemical_list: D002712:Chlorides; D012964:Sodium; D008274:Magnesium; D011188:Potassium