Intrastriatal taurine increases striatal extracellular dopamine in a tetrodotoxin-sensitive manner in rats.
In vivo effects of locally administered taurine on striatal dopamine release and metabolism were studied by microdialysis in freely moving rats. Concentrations of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in striatal dialysates were quantified by high pressure liquid chromatography (HPLC) using electrochemical detection. Infusion of 150 mM taurine into the striatum for 2 h induced a 2.5-fold increase in the extracellular dopamine concentration. Extracellular DOPAC concentration increased nearly 2-fold. Taurine infusion initially decreased HVA to 70% but afterwards increased it to 140% of the control. When taurine was infused simultaneously with 1 microM tetrodotoxin starting 60 min after tetrodotoxin, the output of dopamine did not differ from that in the presence of tetrodotoxin alone. Tetrodotoxin abolished the effects of taurine on dopamine metabolites as well. Tetrodotoxin-sensitivity of the effects of taurine on dopamine and its metabolites suggests that intrastriatal taurine elevates extracellular dopamine by releasing it from neuronal pool.
Ruotsalainen
M
M
Department of Pharmacy, University of Helsinki, Finland. minna.ruotsalainen@helsinki.fi
Ahtee
L
L
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects*; D004298:Dopamine / Q000378:metabolism*; D007278:Injections, Spinal; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology*
D015102:3,4-Dihydroxyphenylacetic Acid; D013654:Taurine; D013779:Tetrodotoxin; D004298:Dopamine