A role for the bulged nucleotide 47 in the facilitation of tertiary interactions in the tRNA structure.
Based on computer modeling and with the use of energy minimisation procedure, we show that the bulged nucleotide 47 in the yeast tRNA(Phe) structure plays an important steric role, allowing the formation of canonical tertiary interactions 15-48 and 22-46 within the D-domain. The absence of nucleotide 47 can be compensated by the presence of a wobble pair U13-G22, whose unusual stereochemistry permits as well the formation of the canonical tertiary interactions. The tRNA database show that the vast majority of the cytosolic tRNAs have either a nucleotide at position 47 or a wobble pair U13-G22. On the contrary, many mitochondrial tRNAs, having a Watson-Crick pair 13-22, do not have nucleotide in position 47, which suggests that their tertiary interactions within the D-domain must differ from those in cytosolic tRNAs.
Steinberg
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Departement de Biochimie, Université de Montréal, Quebéc, Canada.
Ioudovitch
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A
D001483:Base Sequence; D003198:Computer Simulation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry*
D009711:Nucleotides; D012343:RNA, Transfer