Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames.
The nucleotide sequence of a fragment from the left arm of Saccharomyces cerevisiae chromosome VII has been determined. Analysis of the 14,607 bp DNA segment reveals nine open reading frames (ORFs) longer than 300 bp. G2827 is the SEC 7 gene, an essential coatomer complex subunit. G2834 encodes SSM1b, a ribosomal protein. The G2838 product shows homology to hypothetical yeast proteins, YIF0 and YE09, of unknown function. The G2830 product shows homology with the cell division protein FtsJ from Escherichia coli, with two hypothetical proteins from yeast, YCF4 and YBR1, and with R74.7, a hypothetical protein from Caenorhabditis elegans. Two of the ORFs are completely internal to longer ones and a third is partially embedded in G2850. The remaining ORFs give no significant homology with proteins in the databases.
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Instituto de Investigaciones Biomédicas del C.S.I.C., Departmento de Bioquimica, Facultad de Medicina de la U.A.M., Madrid, Spain.
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D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000235:genetics; D015825:Chromosomes, Fungal; D020756:Coatomer Protein; D003360:Cosmids; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008780:Methyltransferases; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins / Q000235:genetics; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D020756:Coatomer Protein; D004271:DNA, Fungal; D005656:Fungal Proteins; D016601:RNA-Binding Proteins; C063003:RPL1A protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C089602:Sec27 protein, S cerevisiae; D012436:S-Adenosylmethionine; D008780:Methyltransferases; C102247:rlmE protein, E coli