Photoreaction of 5'-halouracil-containing DNA. A novel photoproduct at 5'BrU-pyrimidine-3' sequence.
Photoreaction of various 5-bromouracil-containing oligonucleotides was investigated. It was found that a novel type of 5 cross-link products was formed at 5'-BrU-pyridimdine-3' sequence under UV irradiation. These cross-linked products were characterized by 1H NMR and mass spectra. It was found that this type of photo-cross-linking was actually proceeded in BrU-containing hexanucleotides.
Hatano
K
K
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
Sugiyama
H
H
Saito
I
I
D001483:Base Sequence; D001976:Bromouracil / Q000737:chemistry; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D001976:Bromouracil; D004247:DNA