Differential changes in the concentrations of cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in exudate during rat lipopolysaccharide-induced inflammation.
Recently we have purified four neutrophil chemotactic factors from conditioned medium of rat granulation tissue. Two chemokines besides cytokine-induced neutrophil chemoattractant-1 (CINC-1, formerly called CINC) and CNC-3/macrophage inflammatory protein-2 (MIP-2) were novel chemoattractants, designated as CINC-2 alpha and CINC-2 beta. In the present report, we developed an enzyme-linked immunosorbent assay (ELISA) specific for CINC-2. The biotin-streptavidin sandwich ELISA for CINC-2 beta detected CINC-2 alpha and CINC- 2 beta equally well between 1 ug/mI and 300 ng/ml, but did not show cross-reactivity with CINC-1 and CINC-3. The concentrations of CINC-1 and CINC-2 in the exudate during rat lipopolysaccharide (LPS)-induced inflammation were determined. The CINC-1 concentration in the exudate reached a maximum at 4 h after LPS injection, whereas the CINC-2 level steadily increased up to 8 h. The number of infiltrated cells in the exudate increased linearly until 6 h and gradually up to 8 h. Increase in the cell number was correlated with total concentrations of CINC-1 and CINC-2. The results suggest that CINC-2 as well as CINC-1 plays an important role in accumulation of neutrophils into the inflammatory lesion of LPS- induced inflammation in rats.
Shibata
F
F
Department of Physiological Chemistry, Toyama Medical and Pharmaceutical University, Sugitani, Toyana, Japan.
Kato
H
H
Konishi
K
K
Okumora
A
A
Ochiai
H
H
Nakajima
K
K
Al-Mokdad
M
M
Nakagawa
H
H
D000818:Animals; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D018925:Chemokines / Q000032:analysis / Q000096:biosynthesis*; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006099:Granuloma; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis*; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009504:Neutrophils / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification
D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; C513296:Cxcl2 protein, rat; C513294:Cxcl3 protein, rat; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins