Theory of gel electrophoresis of DNA: orientational effects.
Some aspects of the reptation model of gel electrophoresis of DNA, with emphasis on the orientational effects, are considered. A master equation for the reptational dynamics with the end boundary conditions is proposed. Based on this equation, the orientation and dynamics of the persistent-chain are investigated. In particular, the role of trap conformations for this chain, which essentially reduce the chain mobility, was analyzed. A transient orientation of the chain during pulsed field gel electrophoresis is also discussed.
Subbotin
A V
AV
Institute of Petrochemical Synthesis, Russian Academy of Science, Moscow, Russia. tips@tips.rc.ac.ru
Semenov
A N
AN
D004247:DNA / Q000032:analysis*; D004586:Electrophoresis; D016521:Electrophoresis, Gel, Pulsed-Field; D008432:Mathematical Computing; D008958:Models, Molecular
D004247:DNA