Expression of c-fos precedes MDR3 in vincristine and adriamycin selected multidrug resistant murine erythroleukemia cells.
Expression of murine P-glycoprotein (P-gp), encoded by mdrl or mdri3, confers a multidrug resistance phenotype. Higher expression of c-fos and c-jun has also been demonstrated in multidrug resistant human and murine cells. We detected increased expression of c-fos early in the derivation of two series of murine erythroleukemia sublines selected for resistance to vincristine or adriamycin which eventually overexpress mdr3. We speculate that early expression of c-fos prepares cells for overexpression of other genes, such as mdr3, that contribute to the multidrug resistance phenotype.
Bhushan
A
A
Vermont Cancer Center, University of Vermont College of Medicine, Burlington 05405, USA.
Slapak
C A
CA
Levy
S B
SB
Tritton
T R
TR
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis*; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004317:Doxorubicin / Q000633:toxicity*; D018432:Drug Resistance, Multiple; D015870:Gene Expression; D016762:Genes, fos; D016758:Genes, jun; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000633:toxicity*
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014750:Vincristine; D004317:Doxorubicin; C095122:multidrug resistance protein 3