title: Elongation factor TFIIS contains three structural domains: solution structure of domain II.
abstract: Transcription elongation by RNA polymerase II is regulated by the general elongation factor TFIIS. This factor stimulates RNA polymerase II to transcribe through regions of DNA that promote the formation of stalled ternary complexes. Limited proteolytic digestion showed that yeast TFIIS is composed of three structural domains, termed I, II, and III. The two C-terminal domains (II and III) are required for transcription activity. The structure of domain III has been solved previously by using NMR spectroscopy. Here, we report the NMR-derived structure of domain II: a three-helix bundle built around a hydrophobic core composed largely of three tyrosines protruding from one face of the C-terminal helix. The arrangement of known inactivating mutations of TFIIS suggests that two surfaces of domain II are critical for transcription activity.
authors.affiliation: Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada.
authors.forename: P E
authors.initials: PE
authors.lastname: Morin
authors.forename: D E
authors.initials: DE
authors.lastname: Awrey
authors.forename: A M
authors.initials: AM
authors.lastname: Edwards
authors.forename: C H
authors.initials: CH
authors.lastname: Arrowsmith
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins / Q000648:ultrastructure*; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae; D012996:Solutions; D014157:Transcription Factors / Q000648:ultrastructure*; D035165:Transcription Factors, General; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors; D015032:Zinc
chemical_list: D005656:Fungal Proteins; D008667:Metalloproteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012996:Solutions; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D015032:Zinc