title: Uptake and release of dopamine through the rat dopamine transporter expressed in Xenopus laevis oocyte: evaluation by voltammetric measurement of intracellular dopamine concentration.
abstract: An attempt was made to evaluate the uptake and release of dopamine (DA) through the rat dopamine transporter (DAT) expressed in Xenopus laevis oocytes. A carbon electrode was placed intracellularly into the oocyte to measure directly the intracellular concentration of DA ([DA]i) by voltammetric analyzer. Oocytes injected with the rat DAT cRNA showed a rapid rise and fall of [DA]i in response to the perfusion solution in the presence and absence of DA. In the control experiment, oocytes injected with water showed no response to external DA. An increase in [DA]i which was induced in control oocytes injected with DA into cytosol was not affected by DA-free perfusion solution, suggesting that in the absence of the transporter the accumulated DA never leaked out from the cell. Thus, the bi-directional DA fluxes through the transporter were demonstrated electrochemically in real time on-line mode.
authors.affiliation: Department of Pharmacology, Hiroshima University School of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kitayama
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: T
authors.initials: T
authors.lastname: Dohi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D004594:Electrophysiology; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins; D008564:Membrane Potentials / Q000502:physiology; D026901:Membrane Transport Proteins; D008839:Microelectrodes; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493216:Slc6a3 protein, rat; D004298:Dopamine