Protozoal colonization of the intestinal tract in institutionalized Romanian children.
To determine the prevalence of intestinal parasitic infections in 92 Romanian children institutionalized at Colentina Hospital (Bucharest, Romania) and at the Dystrophic Center (Vidra, Romania), medical charts were reviewed and complete physical examinations were performed. The nutritional status of each child was evaluated, and their sera were tested for the presence of antibodies to human immunodeficiency virus (HIV) and Cryptosporidium. Fecal samples were collected in 10% formalin and examined by an immunofluorescent assay and by trichrome staining for intestinal parasites. At least one protozoan was identified in 77% of the fecal specimens examined. Giardia lamblia (72% of cases), Cryptosporidium parvum (12%), and Entamoeba coli (4%) were the only parasites identified. Stepwise logistic regression revealed that the only factors predictive of giardia colonization were normal nutritional status (P < .01) and HIV seropositivity (P < .02), while cryptosporidium colonization was only associated with where the children lived (P < .01). Seventy-three percent of the children had IgA and/or IgG antibodies to Cryptosporidium in their sera. The presence of these antibodies was strongly associated with the severity of symptoms present in the HIV-infected children (P < .01). Protozoal colonization of the intestinal tract is common in institutionalized Romanian children and may play a role in causing morbidity and mortality in this high-risk group of children.
Brannan
D K
DK
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
Greenfield
R A
RA
Owen
W L
WL
Welch
D F
DF
Kuhls
T L
TL
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000276:immunology / Q000469:parasitology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000150:complications / Q000276:immunology / Q000469:parasitology; D003458:Cryptosporidium / Q000276:immunology / Q000302:isolation & purification; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D003967:Diarrhea / Q000150:complications / Q000276:immunology / Q000469:parasitology*; D004404:Dysentery, Amebic / Q000150:complications / Q000276:immunology / Q000469:parasitology; D004747:Entamoeba / Q000302:isolation & purification* / Q000378:metabolism; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000302:isolation & purification* / Q000378:metabolism; D005873:Giardiasis / Q000150:complications / Q000276:immunology / Q000469:parasitology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007411:Intestinal Diseases, Parasitic / Q000150:complications / Q000276:immunology / Q000469:parasitology*; D008297:Male; D009748:Nutrition Disorders / Q000150:complications; D012383:Romania
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G