title: The nanomelic mutation in the aggrecan gene is expressed in chick chondrocytes and neurons.
abstract: We have established the presence of at least two large chondroitin sulfate proteoglycans in the developing chick brain, one that reacts exclusively with HNK-1, a carbohydrate epitope found on several neural specific molecules, and one that reacts with S103L, a defined peptide epitope in the CS-2 domain of the cartilage-specific chondroitin sulfate proteoglycan (CSPG), aggrecan. In order to determine the relationships between the two distinct S103L-reactive CSPGs from cartilage (chondrocytes) and brain (neurons), as well as among the three large CSPGs expressed in brain, S103L, HNK-1 and versican, we studied the expression of these multiple proteoglycan species in the brain of nanomelic chicks. We have previously shown that homozygous embryos expressing the nanomelic phenotype exhibit a single point mutation in the aggrecan gene. In the present study, the S103L CSPG is not accumulated or synthesized by embryonic chick CNS tissue or E8CH neuronal cultures derived from nanomelic chick embryo cerebral hemispheres. In contrast, expression of both versican and the HNK-1 CSPG was normal in the mutant embryo CNS. Pulse chase experiments demonstrated the presence of the 380 kDa precursor in normal neurons and the 300 kDa truncated precursor in nanomelic neurons. Northern blot analysis revealed normal-sized mRNA but reduced levels of expression of the S103L CSPG message in nanomelic neurons, while expression of the versican message was comparable in normal and nanomelic neurons. Most conclusively, the point mutation previously identified in nanomelic cartilage mRNA was also identified in nanomelic brain mRNA. Together these results provide evidence that a single aggrecan gene is expressed in both cartilage and CNS tissue leading to the production of identical core proteins which then undergo differential and tissue-specific post-translation processing, resulting in the characteristic tissue-specific proteoglycans. Furthermore, versican and the HNK-1 CSPG, although structurally and chemically similar to the S103L CSPG, are the products of separate genes.
authors.affiliation: Department of Pediatrics, University of Chicago, IL 60637, USA.
authors.forename: M
authors.initials: M
authors.lastname: Domowicz
authors.forename: R C
authors.initials: RC
authors.lastname: Krueger
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Mangoura
authors.forename: B M
authors.initials: BM
authors.lastname: Vertel
authors.forename: N B
authors.initials: NB
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D018959:CD57 Antigens / Q000096:biosynthesis / Q000235:genetics; D002356:Cartilage / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D018959:CD57 Antigens; D012333:RNA, Messenger; D002809:Chondroitin Sulfates