Separation of anti-shark type I collagen antibody from anti-pig type I collagen antiserum and its partial characterization.
Anti-shark type I collagen antibody (PAb-S) was successfully separated from anti-pig type I collagen antiserum. PAb-S recognized a chymotryptic peptide (29 kDa) of alpha 1 (I) chain prepared from shark skin. This result suggested that the epitope contained in this chymotryptic peptide may be well conserved during the molecular evolution of type I collagen.
Nomura
Y
Y
Laboratory of Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Techology, Japan.
Sasaki
Y
Y
Arai
K
K
Ishii
Y
Y
Shirai
K
K
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D003094:Collagen / Q000276:immunology*; D018604:Epitope Mapping; D012754:Sharks / Q000276:immunology*; D013552:Swine / Q000276:immunology*
D000906:Antibodies; D003094:Collagen