Comparison of peptides eluted from the groove of HLA-B27 from Salmonella infected and non-infected cells.
Reactive arthritis (ReA) is associated with the MHC class-I molecule HLA-B27 and caused by certain Gram-negative bacteria. The mechanism by which HLA-B27 confers a higher susceptibility for this disease compared to other MHC Class-I alleles is still not known. We investigated whether infection of human HLA-B27+ cells is able to change the peptide repertoire presented by these HLA-B27 molecules. To this end large quantities of a B-cell line (C1R-B27) transfected with HLA-B2705 were infected with S. typhimurium. Peptides were eluted from the B27 molecules and separated by Reversed Phase Chromatography (RPC). We then compared the peptide profiles obtained from S. typhimurium infected CIR B-cells with that obtained from non infected cells. Apart from a few additional peaks present in the profile derived from the infected batch the peptide profiles were almost identical. A few fractions were subjected to sequencing by Edman degradation. All peptides found were nonameres with arginine (Arg) at position 2 which is in agreement with the previously described HLA-B27 peptide binding motif. The majority of peaks expressed a mixture of at least four different peptides. The analysis of differences between HLA-B27 bound peptides from Salmonella infected and non infected cells might lead to the identification of T-cell epitopes shared by Salmonella and autoantigens.
Ringrose
J H
JH
Netherlands Ophthalmic Research Institute, Amsterdam.
Yard
B A
BA
Muijsers
A
A
Boog
C J
CJ
Feltkamp
T E
TE
D016918:Arthritis, Reactive / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000145:classification / Q000276:immunology*; D015796:HLA-B27 Antigen / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000145:classification* / Q000378:metabolism; D000091402:Prohibitins; D012480:Salmonella Infections / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D010455:Peptides; D000091402:Prohibitins