title: Postabsorptive factors are important for satiation in rats after a lipid meal.
abstract: We examined satiation after intraduodenal infusion of triglyceride with or without Pluronic L-81 (L-81) in rats. L-81 suppresses lipid transport into mesenteric lymph by preventing the formation of chylomicrons. Under unrestrained conditions, a lipid emulsion was infused into 24-h fasted rats intraduodenally for 8 h. After the infusion, food intake during the first 30 min and blood samples were measured. Food intake after the 8-h lipid infusion decreased significantly (-75%) compared with that of rats infused with vehicle only, and the effect of the lipid meal was time dependent. The satiation effect of the lipid was lost as a result of L-81 treatment, but food intake was still significantly decreased (-22%). The loss of inhibition of food intake by the feeding of lipid plus L-81 was totally reversed by the cessation of L-81 infusion. In rats infused with the lipid emulsion, plasma levels of triglyceride and apolipoprotein A-IV increased significantly. This increase was abolished by the presence of L-81 in the lipid infusate. The present study indicates that postabsorptive factors are important for anorexia observed after a lipid meal in addition to preabsorptive factors. This study further supports our previous conclusion that apolipoprotein A-IV is an important postabsorptive factor for satiation after a lipid meal.
authors.affiliation: Department of Internal Medicine, Saga Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakata
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: S
authors.initials: S
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: K
authors.initials: K
authors.lastname: Fukagawa
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: P
authors.initials: P
authors.lastname: Tso
mesh_terms: D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001054:Apolipoproteins A; D004041:Dietary Fats; C022205:apolipoprotein A-IV