title: Corticotropin-releasing factor (CRF) receptors in infant rhesus monkey brain and pituitary gland: biochemical characterization and autoradiographic localization.
abstract: A large body of data suggests that the corticotropin-releasing factor (CRF) system serves to coordinate the autonomic, endocrine, immune and behavioral aspects of the stress response. In rats, the distribution of CRF receptors in brain and pituitary has been well characterized, however, little information is available in primates. In this study, CRF receptors were characterized by radioligand binding and localized using autoradiography with [125I]-oCRF in the pituitary gland and in discrete brain regions of 2-week-old, 12-week-old and adult rhesus monkeys. Autoradiographic localization studies in slide-mounted tissue sections in the 2- and 12-week-old monkeys demonstrated high CRF receptor densities in both anterior and intermediate lobes of the pituitary as well as in discrete regions of the brain. The distribution of CRF receptors in the anterior pituitary demonstrated a 'cluster-like' appearance reminiscent of corticotrope distribution. In contrast, receptors in the intermediate lobe were more uniformly distributed. No significant differences were evident in the pattern of localization or the number of CRF receptors in the pituitaries of 2- compared to 12-week-old animals. However, marked differences were observed in the dentate gyrus of the hippocampus. Receptors in this region were absent in 1- to 2-week old animals but quite dense by 11-12 weeks of age. Conversely, in the lateral and medial geniculate nuclei, high levels of CRF receptors were identified early in life that virtually disappeared by 11-12 weeks of age. Thus, there is considerable correspondence in the development of the CRF system between the rat and rhesus monkey and this presents further evidence for the functional role of this peptide in brain development.
authors.affiliation: Neurocrine Biosciences Inc., San Diego, CA 92121, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Grigoriadis
authors.forename: G W
authors.initials: GW
authors.lastname: Dent
authors.forename: J G
authors.initials: JG
authors.lastname: Turner
authors.forename: H
authors.initials: H
authors.lastname: Uno
authors.forename: S E
authors.initials: SE
authors.lastname: Shelton
authors.forename: E B
authors.initials: EB
authors.lastname: De Souza
authors.forename: N H
authors.initials: NH
authors.lastname: Kalin
mesh_terms: D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D005260:Female; D007457:Iodine Radioisotopes; D008253:Macaca mulatta; D008566:Membranes / Q000378:metabolism; D010902:Pituitary Gland / Q000033:anatomy & histology / Q000378:metabolism*; D011869:Radioligand Assay; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone