title: Expression of truncated midkine in human colorectal cancers.
abstract: Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transcription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated from of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.
authors.affiliation: Department of Oncogene Research, Osaka University, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Miyashiro
authors.forename: T
authors.initials: T
authors.lastname: Kaname
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: S
authors.initials: S
authors.lastname: Nakamori
authors.forename: T
authors.initials: T
authors.lastname: Yagyu
authors.forename: T
authors.initials: T
authors.lastname: Monden
authors.forename: N
authors.initials: N
authors.lastname: Kikkawa
authors.forename: I
authors.initials: I
authors.lastname: Nishisho
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: M
authors.initials: M
authors.lastname: Monden
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D016207:Cytokines; D015870:Gene Expression; D006801:Humans; D000078792:Midkine; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; D012333:RNA, Messenger; D000078792:Midkine