Sorting by COP I-coated vesicles under interphase and mitotic conditions.
COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N-acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when the vesicles were prepared under interphase or mitotic conditions showing that sorting was unaffected. The average density relative to starting membranes for resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%) provides clues to the function of COP I vesicles in transport through the Golgi apparatus.
Sönnichsen
B
B
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Watson
R
R
Clausen
H
H
Misteli
T
T
Warren
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology; D006056:Golgi Apparatus / Q000737:chemistry / Q000201:enzymology; D007150:Immunohistochemistry; D007399:Interphase / Q000502:physiology*; D007425:Intracellular Membranes / Q000737:chemistry* / Q000201:enzymology; D008099:Liver / Q000166:cytology; D008361:Mannosidases / Q000032:analysis; D016253:Microscopy, Immunoelectron; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism; D051381:Rats
D001426:Bacterial Proteins; D011506:Proteins; C065826:cop protein, Bacteria; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase