Monoamine oxidase gene transcription in human cell lines: treatment with psychoactive drugs and ethanol.
In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion.
Ekblom
J
J
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, USA.
Zhu
Q S
QS
Chen
K
K
Shih
J C
JC
D000428:Alcohol Drinking; D018692:Antimanic Agents / Q000494:pharmacology; D014150:Antipsychotic Agents / Q000494:pharmacology; D001254:Astrocytoma; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D018021:Lithium Chloride / Q000494:pharmacology; D008995:Monoamine Oxidase / Q000235:genetics*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009447:Neuroblastoma; D011401:Promoter Regions, Genetic / Q000502:physiology; D011619:Psychotropic Drugs / Q000494:pharmacology*; D020891:Raclopride; D012457:Salicylamides / Q000494:pharmacology; D012642:Selegiline / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects* / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D018692:Antimanic Agents; D014150:Antipsychotic Agents; D002492:Central Nervous System Depressants; D008996:Monoamine Oxidase Inhibitors; D011619:Psychotropic Drugs; D012457:Salicylamides; D012642:Selegiline; D000431:Ethanol; D020891:Raclopride; D008995:Monoamine Oxidase; D018021:Lithium Chloride