Detection and determination of the major metabolites of [3H]cytosine arabinoside by high-performance liquid chromatography.
An ion-pair high-performance liquid chromatographic assay involving solid-phase scintillation detection was established for the rapid identification and determination of all major metabolites of tritium-labelled cytosine arabinoside (Ara-C) in an in vitro system. In a single run of 50 min, Ara-C, Ara-CMP, Ara-CDP-choline, Ara-CDP, Ara-U, Ara-UMP, Ara-CTP, Ara-UDP and Ara-UTP can be measured. The method is fast, sensitive, with limits of detection ranging from 40 to 200 pg (absolute), and highly reproducible.
Braess
J
J
Department of Hematology and Oncology, University of Göttingen, Germany.
Pförtner
J
J
Kaufmann
C C
CC
Ramsauer
B
B
Unterhalt
M
M
Hiddemann
W
W
Schleyer
E
E
D002138:Calibration; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003561:Cytarabine / Q000032:analysis* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D003561:Cytarabine