Inhibition of motility and invasion of human lung cancer cells by invasion inhibiting factor 2.
The motility and invasion of cancer cells are basic requirements for the establishment of distant metastases. In this study, we examined the effect of invasion inhibiting factor 2 (IIF2), a motility/invasion regulatory agent, on the motility, invasion, growth and basement membrane attachment of human lung cancer cells. IIF2 significantly reduced cell dissociation, colony scattering and invasion induced by the motogenic factor, HGF/SF. Western and Northern analyses showed these cells to be positive for the HGF/SF receptor c-met. These effects were blocked by an anti-IIF2 antibody. IIF2 did not affect the growth and attachment of lung cancer cells to the basement membrane. It is concluded therefore that invasion inhibiting factor 2 is an inhibitor of human lung cancer cell motility and invasion in vitro and this may bear some importance in the construction of anti-metastatic therapies.
Han
Z G
ZG
Department of Thoracic Surgery, Third Teaching Hospital, Norman Bethune University of Medical Sciences, Changchun Jilin, China.
Jiang
W G
WG
Hiscox
S
S
Hallett
M B
MB
Isoai
A
A
Mansel
R E
RE
D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors*; D020533:Angiogenesis Inhibitors; D001485:Basement Membrane / Q000473:pathology; D015152:Blotting, Northern; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D011506:Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors; D011506:Proteins