Sensitive high-performance thin-layer chromatography method for detection and determination of ranitidine in plasma samples.
A high-performance thin-layer chromatographic procedure has been developed for the determination of ranitidine, a H2-receptor antagonist, in plasma. The detection and quantification were performed without using internal standards. A single-stage extraction procedure was followed for extracting ranitidine from plasma, and a known amount of the extract was spotted on precoated silica gel F254 plates. Ranitidine was quantified using a Shimadzu CS930 dual-wavelength TLC scanner. The method provides a direct estimate of total ranitidine present in the plasma.
Mody
V D
VD
R&D Centre, Cadila Labs. Ltd, Ahmedabad, India.
Satia
M C
MC
Gandhi
T P
TP
Modi
I A
IA
Modi
R I
RI
Chakravarthy
B K
BK
D000328:Adult; D001682:Biological Availability; D002138:Calibration; D002855:Chromatography, Thin Layer; D006207:Half-Life; D006635:Histamine H2 Antagonists / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011899:Ranitidine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards
D006635:Histamine H2 Antagonists; D007202:Indicators and Reagents; D011899:Ranitidine