Long-term regulation of synapsin I gene expression and neuronal morphology by cyclic AMP and low-dose staurosporine.
We examined the phenotypic changes of neuroblastoma cells chronically treated with cAMP and nanomolar concentrations of staurosporine. These agents, given together, produced cells with a neuronal morphology and a delayed increase (approximately 10 days) in synapsin I mRNA levels. Dopamine-beta hydroxylase mRNA was upregulated within 24 h. We provide evidence that low-dose staurosporine acts cooperatively with cyclic AMP in the acquisition of mature neuronal phenotypes.
Sasaki
K
K
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan. ksasaki@ncc.go.jp
Tsukada
T
T
Maruyama
K
K
Yamaguchi
K
K
D000262:Adenylyl Cyclases / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D017931:DNA Primers; D004299:Dopamine beta-Hydroxylase / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D019311:Staurosporine / Q000494:pharmacology*; D016704:Synapsins / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D016704:Synapsins; D005576:Colforsin; D000242:Cyclic AMP; D004299:Dopamine beta-Hydroxylase; D000262:Adenylyl Cyclases; D019311:Staurosporine