title: Production of monoclonal antibodies against classical swine fever virus and their use for antigenic characterization of Japanese isolates.
abstract: Twenty monoclonal antibodies (MAbs) were produced against the virulent ALD or vaccinal GPE- strains of classical swine fever (CSF) virus. The MAbs were divided into 7 groups on the basis of the difference in the reaction spectrum to various CSF and bovine viral diarrhea-mucosal disease (BVD-MD) viruses. The panel of the MAbs could classify 20 CSF viruses into 6 types, and could discriminate between not only CSF and BVD-MD viruses but also the vaccinal GPE- strain and the field isolates of CSF virus. The results indicated that the MAbs were useful for diagnosis and investigation of CSF viruses.
authors.affiliation: Hokkaido Branch Laboratory, National Institute of Animal Health, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishimori
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis; D002417:Cattle; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000276:immunology; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification; D007564:Japan; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal