title: Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA.
abstract: We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP analysis alone with DNA probe hMFl 87% of 244 donor/recipient pairs revealed different specific bands. By examination of the identical DNA probes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations can be monitored using a combination of RFLP and PCR-AFLP analyses at one locus.
authors.affiliation: Red Cross Blood Center, University of Ulm, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Schreiner
authors.forename: H
authors.initials: H
authors.lastname: Prochnow-Calzia
authors.forename: B
authors.initials: B
authors.lastname: Maccari
authors.forename: E
authors.initials: E
authors.lastname: Erne
authors.forename: I
authors.initials: I
authors.lastname: Kinzler
authors.forename: A
authors.initials: A
authors.lastname: Wölpl
authors.forename: M
authors.initials: M
authors.lastname: Wiesneth
mesh_terms: D016026:Bone Marrow Transplantation / Q000276:immunology*; D002678:Chimera / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014184:Transplantation, Homologous
chemical_list: D004247:DNA