title: Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope.
abstract: Classical swine fever virus (CSFV)-specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4(-)CD6+CD8+ MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Pauly
authors.forename: K
authors.initials: K
authors.lastname: Elbers
authors.forename: M
authors.initials: M
authors.lastname: König
authors.forename: T
authors.initials: T
authors.lastname: Lengsfeld
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000502:physiology; D002461:Cell Line, Transformed; D006691:Classical Swine Fever / Q000276:immunology; D006692:Classical Swine Fever Virus / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D013552:Swine; D013556:Swine, Miniature; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; C062279:CD6 antigen; D016827:CD8 Antigens; D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D017361:Viral Nonstructural Proteins