Immunomodulatory effect of some amphiphilic detergents on the human promyelocytic HL-60 cells.
The effect of two anionic (sodium dodecyl sulfate (SDS) and sodium laurate), two cationic (didodecyldimethylammonium bromide (DDAB) and [(1-methyldodecyl)-trimethylammonium bromide (2-ATDBr)] and a nonionic [(1-methyldodecyl)-dimethyl-amine N-oxide (2-ATDNO)] amphiphilic detergents on the metabolic activation of phagocytes derived from human promyelocytic leukemia cell line HL-60 was investigated. SDS in the concentrations of 10(-4) M significantly stimulated the INT-reduction and superoxide production by HL-60 cells which were preincubated with phorbol 12-myristate 13-acetate (PMA) for a macrophage-like differentiation. This effect was not significant in the case of cells differentiated along monocyte-like and granulocyte-like pathways. A similar influence was observed by sodium laurate, but this effect was significant (P < 0.01) by INT-reduction only. Concentrations higher than 5 x 10(-5) M DDAB, 2-ATDBr and 2-ATDNO inhibited INT-reduction and superoxide generation in HL-60 cells differentiated along all three ways. Our results indicated that differentiated HL-60 cells may be used for in vitro testing of immunotoxic effects on respiratory burst in phagocytes.
Kacáni
L
L
Institute of Immunology, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Ferencík
M
M
Devínský
F
F
Dierich
M P
MP
D003902:Detergents / Q000494:pharmacology*; D004123:Dimethylamines / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007850:Lauric Acids / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013936:Thymidine / Q000493:pharmacokinetics; D014316:Tritium
D003902:Detergents; D004123:Dimethylamines; D007850:Lauric Acids; D000644:Quaternary Ammonium Compounds; D013778:Tetrazolium Salts; D014316:Tritium; D013481:Superoxides; C030358:lauric acid; C046112:didodecyldimethylammonium; C013574:iodonitrotetrazolium; D012967:Sodium Dodecyl Sulfate; C060937:N-(1-methyldodecyl)-N,N,N-trimethylammonium; C019353:N,N-dimethyl-1-methyldodecylamine oxide; D013936:Thymidine