title: Oxidation of D(-)3-hydroxybutyrate administered to rats with extensive burns.
abstract: Although the suppressive effect of 3-hydroxybutyrate (3-OHB) on post-traumatic protein catabolism in traumatized patients is well documented, the oxidation of exogenously administered 3-OHB during catabolic stress has not been investigated. The present study was designed to evaluate, using radioactive isotopes, total body oxidation in rats with and without burn stress to which 3-OHB had been exogenously administered, in comparison with total body oxidation in such rats that had received glucose. The rats were divided into four groups, based on whether or not a 30% full-thickness burn was inflicted, and the type of infusate they received after the burn, namely, 3-OHB or glucose. The total exhaled CO2 was collected for 6h after the infusion was commenced, and 14CO2 was assayed in a liquid scintillation spectrometer. Oxidation of the infusate was calculated from the percentage of exhaled 14CO2 derived from the infused substrates. The plasma concentration of 3-OHB was significantly increased after the infusion in both the burned and non-burned rats. The total exhaled 14CO2 from the rats infused with glucose decreased from 48.2 +/- 2.4% to 40.8 +/- 3.7% (means +/- SD, P < 0.001) after thermal injury. However, the total exhaled 14CO2 from the rats infused with 3-OHB appeared sooner, and there was no difference in the total expired 14CO2 derived from 3-OHB between the burned and non-burned rats, at 68.1 +/- 2.7% vs 66.4 +/- 3.4%, respectively. These findings suggest that even under conditions of burn stress, 3-OHB can be oxidized normally if the plasma concentration of 3-OHB is elevated by exogenous administration.
authors.affiliation: Department of Traumatology, Osaka University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizobata
authors.forename: A
authors.initials: A
authors.lastname: Hiraide
authors.forename: M
authors.initials: M
authors.lastname: Katayama
authors.forename: H
authors.initials: H
authors.lastname: Sugimoto
authors.forename: T
authors.initials: T
authors.lastname: Yoshioka
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000628:therapy; D002245:Carbon Dioxide / Q000502:physiology; D002250:Carbon Radioisotopes; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D006885:Hydroxybutyrates / Q000008:administration & dosage / Q000378:metabolism*; D007262:Infusions, Intravenous; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002250:Carbon Radioisotopes; D006885:Hydroxybutyrates; D002245:Carbon Dioxide; D005947:Glucose; D020155:3-Hydroxybutyric Acid