Cobalt but not hypoxia stimulates PDGF gene expression in rats.
This study was done to investigate the influence of different forms of acute tissue hypoxia on the expression of platelet-derived growth factor (PDGF) A chain (PDGF-A) and PDGF B chain (PDGF-B) genes in different rat organs. We found that acute normobaric hypoxia (8% O2), carbon monoxide inhalation (0.1% CO), or lowering the hematocrit to 12% for 6 h had no effect on PDGF-A or PDGF-B gene expression in lung, heart, kidney, and liver of Sprague-Dawley rats. Subcutaneous administration of cobaltous chloride dose dependently increased PDGF-B mRNA by 125% in lungs, by 60% in kidneys, but not in heart and liver. These findings suggest that acute tissue hypoxygenation is not a significant stimulus for PDGF-A and PDGF-B gene expression in these major rat organs. Cobalt appears to cause a tissue-specific increase of PDGF-B gene expression.
Bucher
M
M
Institut für Physiologie, Universität Regensburg, Germany.
Sandner
P
P
Wolf
K
K
Kurtz
A
A
D000279:Administration, Cutaneous; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage*; D003035:Cobalt / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000187:drug effects*; D000860:Hypoxia; D008297:Male; D009928:Organ Specificity; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016587:Antimutagenic Agents; D010982:Platelet-Derived Growth Factor; D003035:Cobalt; C018021:cobaltous chloride