title: The porcine gene TBP10 encodes a protein homologous to the human tat-binding protein/26S protease subunit family.
abstract: We have cloned a porcine gene, designated TBP1O, that belongs to the Tat-binding protein/26S protease subunit family. The genomic structure of the porcine TBP1O gene was analyzed after isolation of three overlapping genomic phage lambda clones. The TBP10 gene harbors 12 exons spanning 4.5 kb of chromosomal DNA. The TBP1O gene was assigned to Chromosome (Chr) 12 by fluorescence in situ hybridization (FISH) on metaphase chromosomes. The chromosomal location was confirmed by PCR analysis of a porcine-rodent hybrid cell panel. The TBP1O protein is encoded by a 1221 nucleotide cDNA and has a molecular mass of 45.6 kDa. The predicted amino acid sequence has highest similarity to the human and bovine p45 subunit of the 26S protease and the human transcription factor TRIP1. Further similarities were detected to the slime mold protein DdTBP1O and the Schizosaccharomyces pombe and Saccharomyces cerevisiae protein SUG1. Like DdTBP1O and other members of the protein family, the porcine TBP1O harbors a leucine zipper motif in the N-terminal region and a domain characteristics of ATP-dependent proteases in the C-terminal region.
authors.affiliation: Institute of Veterinary Medicine, University of Goettingen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Leeb
authors.forename: G
authors.initials: G
authors.lastname: Rettenberger
authors.forename: J
authors.initials: J
authors.lastname: Breech
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: B
authors.initials: B
authors.lastname: Brenig
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes / Q000235:genetics; D006801:Humans; D060588:LIM Domain Proteins; D016350:Leucine Zippers; D008099:Liver; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D029867:Xenopus Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C092282:PSMC5 protein, human; C105733:TBP-10 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000074183:ATPases Associated with Diverse Cellular Activities