Excitation and sensitization of the heat response induced by a phorbol ester in canine visceral polymodal receptors studied in vitro.
To clarify the possible involvement of protein kinase (PK) C activation in the excitation and sensitization of polymodal receptors (PMRs), the effects of phorbol 12,13-dibutyrate (PDBu) on PMRs were studied in canine testis-spermatic nerve preparations in vitro. Application of PDBu (10(-7), 10(-6), AND 10(-5) M) for 5 min evoked a significant increase in the ongoing activity of the PMRs within 15 min. PDBu (10(-8) to 10(-5) M) significantly augmented the subsequent heat responses of the PMRs. Staurosporine (10(-6) M), a PK inhibitor, attenuated the effect of PDBu on heat responses. These data suggest that activation of PKC contributes to the activities of PMRs.
Leng
S
S
Department of Neural Regulation, Nagoya University, Japan.
Mizumura
K
K
Koda
H
H
Kumazawa
T
T
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature / Q000009:adverse effects*; D066298:In Vitro Techniques; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology*; D013085:Spermatic Cord / Q000187:drug effects / Q000294:innervation / Q000502:physiology; D019311:Staurosporine; D013737:Testis / Q000187:drug effects / Q000294:innervation / Q000502:physiology
D000470:Alkaloids; D004791:Enzyme Inhibitors; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D019311:Staurosporine