title: Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1.
abstract: The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.
authors.affiliation: National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: R
authors.initials: R
authors.lastname: Ayyagari
authors.forename: A
authors.initials: A
authors.lastname: Nestorowicz
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Chandrasekharappa
authors.forename: C
authors.initials: C
authors.lastname: Chinault
authors.forename: P
authors.initials: P
authors.lastname: van Tuinen
authors.forename: R J
authors.initials: RJ
authors.lastname: Smith
authors.forename: J F
authors.initials: JF
authors.lastname: Hejtmancik
authors.forename: M A
authors.initials: MA
authors.lastname: Permutt
mesh_terms: D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006946:Hyperinsulinism / Q000235:genetics*; D016324:Sequence Tagged Sites
chemical_list: D017931:DNA Primers; D005819:Genetic Markers