[Plasmid size determination using 3 methods].
Analysis of bacterial plasmid profiles has been shown to be very important in epidemiological studies, especially those involving outbreaks of nosocomial infections. The molecular weight size of unknown plasmids is determined by comparing their band pattern obtained in agarose gel electrophoresis with those obtained with plasmids that have been used as molecular weight or size standards. In this study, we determined the size of plasmids present in clinical samples of Enterobacter cloacae comparing their electrophoresis mobility with seven plasmids of known size, using three different mathematical methods. For plasmids with molecular weight ranging from 2 kb to 100 kb. The most accurate determinations were obtained by power-function. Analyses using the exponential variables obtained with these plasmids were accurate for two types of plasmids, those with size ranging from 50 kb to 100 kb and those with size ranging from 2 kb to 30 kb. We also observed discrepancies among the methodologies described, including one used by a computer software designed for calculating the size of plasmids DNA.
Darini
A L
AL
Departamento de Análises Clínicas, USP, Brasil.
Zucchi
O L
OL
Carbonell
G V
GV
Furlanetto
A
A
Levy
C E
CE
D000465:Algorithms; D004269:DNA, Bacterial / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D016972:Enterobacter cloacae / Q000648:ultrastructure*; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics / Q000648:ultrastructure*
D004269:DNA, Bacterial