title: Heterogeneity of rearranged T cell receptor V alpha and V beta gene transcripts in synovial fluid T cells of HLA-B27 positive reactive arthritis patients.
abstract: To analyze the T cell receptor (TCR) V-alpha/beta gene usage by synovial fluid (SF) and peripheral blood (PB) T cells of HLA-B27+ reactive arthritis (ReA) patients. The TCR V-alpha/beta gene usage was determined by the polymerase chain reaction on freshly isolated SF and PB mononuclear cells (MNC) of five HLA-B27+ ReA patients. A total of 30 TCR V alpha and 23 V beta (sub)family specific primers in combination with a C alpha or C beta specific primer, respectively, were used. In five patients most of the TCR V alpha and V beta gene segments expressed by PB T cells were also detected in the paired SF samples. Although one patient showed an increased expression of TCR V alpha2 in SF when compared to PB, the SF samples showed a heterogeneous TCR V-gene repertoire similar to PB. Although this study was limited to a small group of patients, the apparent lack of a restricted TCR V-gene repertoire in SF does not support the involvement of a single or limited number of T cell subsets in the disease process of HLA-B27+ ReA patients.
authors.affiliation: Department of Virology, Erasmus University of Rotterdam, Netherlands.
authors.forename: G M
authors.initials: GM
authors.lastname: Verjans
authors.forename: V N
authors.initials: VN
authors.lastname: Klaren
authors.forename: M
authors.initials: M
authors.lastname: Leirisalo-Repo
authors.forename: J H
authors.initials: JH
authors.lastname: Ringrose
authors.forename: H
authors.initials: H
authors.lastname: Repo
authors.forename: A
authors.initials: A
authors.lastname: Steinle
authors.forename: C E
authors.initials: CE
authors.lastname: Van Doornik
authors.forename: T E
authors.initials: TE
authors.lastname: Feltkamp
mesh_terms: D000328:Adult; D016918:Arthritis, Reactive / Q000276:immunology*; D015139:Blotting, Southern; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D015796:HLA-B27 Antigen / Q000276:immunology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D000091402:Prohibitins; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000096:biosynthesis*; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology
chemical_list: D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D000091402:Prohibitins; D011948:Receptors, Antigen, T-Cell