Properties of nicked and circular dumbbell RNA-DNA chimeric oligonucleotides.
We have designed a new type of antisense oligodeoxyribonucleotide. These oligonucleotides form two hairpin loop structures with RNA-DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem. The nicked and circular dumbbell RNA/DNA chimeric oligonucleotides are molecules, with or without free ends, that are more resistant to exonuclease attack. Of particular interest, antisense DNA is liberated by RNase H treatment of the dumbbell RNA/DNA chimeric oligonucleotides.
Yamakawa
H
H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Hosono
K
K
Ishibashi
T
T
Takai
K
K
Takaku
H
H
D001483:Base Sequence; D001665:Binding Sites; D002678:Chimera; D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D000079962:RNA, Circular; D016914:Ribonuclease H
D004270:DNA, Circular; D016376:Oligonucleotides, Antisense; D000079962:RNA, Circular; D012313:RNA; D004247:DNA; D016914:Ribonuclease H