title: Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum.
abstract: Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty-eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.
authors.affiliation: School of Pharmaceutical Sciences, University of Science Malaysia, Minden, Filaria, Malaysia.
authors.forename: H H
authors.initials: HH
authors.lastname: Ang
authors.forename: K L
authors.initials: KL
authors.lastname: Chan
authors.forename: J W
authors.initials: JW
authors.lastname: Mak
mesh_terms: D000818:Animals; D004586:Electrophoresis; D005838:Genotype; D018086:Glutamate Dehydrogenase (NADP+) / Q000145:classification / Q000302:isolation & purification*; D007527:Isoenzymes / Q000145:classification / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D018086:Glutamate Dehydrogenase (NADP+)