High-performance liquid chromatography of fullerence (C60) in plasma using ultraviolet and mass spectrometric detection.
Fullerence (C60) was determined by high-performance liquid chromatography using both ultraviolet and mass spectrometric detection. The detection limit for each method was 0.05 and 2.0 ng (signal-to-noise ratio (S/N = 2)) per injection, respectively. Rat plasma spiked with C60 (10 micrograms/ml) was extracted using solid phase extraction with a recovery of 62.1% and the coefficient of variation (c.v., n = 5) between intra-day assays was 4.0%. The calibration curve for peak area and plasma C60 concentration with ultraviolet detection showed good linearity (r = 0.996) over the range 0.5-60 micrograms/ml. This newly developed method was applied to rat plasma samples after intravenous administration of C60 solubilized with polyvinylpyrrolidone.
Santa
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Yoshioka
D
D
Homma
H
H
Imai
K
K
Satoh
M
M
Takayanagi
I
I
D000818:Animals; D000998:Antiviral Agents / Q000097:blood*; D002138:Calibration; D002244:Carbon / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D037741:Fullerenes; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
D000998:Antiviral Agents; D037741:Fullerenes; D002244:Carbon; C069837:fullerene C60