Enzymatic synthesis of 4-O-beta-D-galactopyranosyl-moranoline and 3-O-beta-D-galactopyranosyl-moranoline by using beta-galactosidase from Bacillus circulans.
A transgalactosylation reaction from lactose to moranoline (1-deoxynojirimycin) was accomplished by using beta-galactosidase [EC 3.2.1.23] from Bacillus circulans. The enzyme formed 3-O-beta-D-galactopyranosyl-moranoline and 4-O-beta-D-galactopyranosyl-moranoline as major products, together with 2-O-beta-D-galactopyranosyl-moranoline and 6-O-beta-D-galactopyranosyl-moranoline as minor ones.
Kojima
M
M
Discovery Research Lab. III, Nippon Shinyaku Co., Ltd., Kyoto Japan.
Seto
T
T
Kyotani
Y
Y
Ogawa
H
H
Kitazawa
S
S
Mori
K
K
Maruo
S
S
Ohgi
T
T
Ezure
Y
Y
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
C101238:3-O-galactopyranoylmoranoline; C101237:4-O-galactopyranosylmoranoline; D017485:1-Deoxynojirimycin; D001616:beta-Galactosidase