Simultaneous determination of 5-aminosalicylic acid, acetyl-5-aminosalicylic acid and 2,5-dihydroxybenzoic acid in endoscopic intestinal biopsy samples in humans by high-performance liquid chromatography with electrochemical detection.
A high-performance liquid chromatographic method for the simultaneous determination of 5-aminosalicylic acid (5-ASA), acetyl-5-aminosalicylic acid (Ac-5-ASA) and 2,5-dihydroxybenzoic acid (5-HSA) in human endoscopic intestinal biopsy with electrochemical detection has been developed and validated. A liquid-liquid extraction procedure was used to isolate these drugs from the biological material prior to analysis. The compounds were separated on an Erbasil S reversed-phase column using methanol-critic acid-sodium hydrogenphosphate-heptane-sulfonic acid-disodium ethylenediaminetetraacetate (pH 3) as mobile phase. The method was linear from 1.0 to 300 ng ml-1 for 5-ASA, from 10 to 1000 ng ml-1 for Ac-5ASA and from 0.1 to 10 ng m-1 for 5-HSA. The limit of detection for 5-ASA and for Ac-5-ASA was 1 ng ml-1 and that for 5-HSA was 0.1 ng ml-1. This procedure is suitable for pharmacological and clinical studies of 5-ASA.
Palumbo
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G
Department of Internal Medicine, University of L'Aquila, Italy.
Carlucci
G
G
Mazzeo
P
P
Frieri
G
G
Pimpo
M T
MT
Fanini
D
D
D000636:Aminosalicylic Acids / Q000032:analysis*; D001706:Biopsy; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005841:Gentisates; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis; D007422:Intestines / Q000737:chemistry*; D019804:Mesalamine
D000636:Aminosalicylic Acids; D005841:Gentisates; D062385:Hydroxybenzoates; D019804:Mesalamine; C028836:N-acetyl-5-aminosalicylic acid; C010925:2,5-dihydroxybenzoic acid