title: Human blood-mobilized hematopoietic precursors differentiate into osteoclasts in the absence of stromal cells.
abstract: Osteoclastogenesis is a complex process that is facilitated by bone marrow stromal cells (SCs). To determine if SCs are an absolute requirement for the differentiation of human hematopoietic precursors into fully mature, osteoclasts (OCs), CD34+ cells were mobilized into the peripheral circulation with granulocyte colony-stimulating factor, harvested by leukapheresis, and purified by magnetic-activated cell sorting. This procedure yields a population of CD34+ cells that does not contain SC precursors, as assessed by the lack of expression of the SC antigen Stro-1, and that differentiates only into hematopoietic cells. We found that CD34+, Stro-1- cells cultured with a combination of granulocyte/macrophage colony-stimulating factor, interleukin 1, and interleukin 3 generated cells that fulfill current criteria for the characterization of OCs, including multinucleation, presence of tartrate-resistant acid phosphatase, and expression of the calcitonin and vitronectin receptors and of pp60c-src tyrosine kinase. These OCs also expressed mRNA for the noninserted isoform of the calcitonin receptor and excavated characteristic resorption pits in devitalized bone slices. These data demonstrate that accessory SCs are not essential for human osteoclastogenesis and that granulocyte colony-stimulating factor treatment mobilizes OC precursors into the peripheral circulation.
authors.affiliation: Division of Bone and Mineral Disease, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: A
authors.initials: A
authors.lastname: Matayoshi
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: J F
authors.initials: JF
authors.lastname: DiPersio
authors.forename: J
authors.initials: J
authors.lastname: Haug
authors.forename: Y
authors.initials: Y
authors.lastname: Abu-Amer
authors.forename: H
authors.initials: H
authors.lastname: Liapis
authors.forename: R
authors.initials: R
authors.lastname: Kuestner
authors.forename: R
authors.initials: R
authors.lastname: Pacifici
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D018952:Antigens, CD34; D001862:Bone Resorption; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D015870:Gene Expression; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007937:Leukapheresis; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D018003:Receptors, Calcitonin / Q000235:genetics; D019030:Receptors, Vitronectin / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D018952:Antigens, CD34; D016207:Cytokines; D007527:Isoenzymes; D018003:Receptors, Calcitonin; D019030:Receptors, Vitronectin; D016179:Granulocyte Colony-Stimulating Factor; D016392:Proto-Oncogene Proteins pp60(c-src); D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase