The mouse thymosin beta 4 gene: structure, promoter identification, and chromosome localization.
Thymosin beta 4 (T beta 4) is an actin monomer sequestering protein that may have a critical role in modulating the dynamics of actin polymerization and depolymerization in nonmuscle cells. Its regulatory role is consistent with the many examples of transcriptional regulation of T beta 4 and of tissue-specific expression. Furthermore, lymphocytes have a unique T beta 4 transcript relative to the ubiquitous transcript found in many other tissues and cells. To determine how T beta 4 gene expression is regulated and how the alternative transcripts are derived, we cloned the mouse T beta 4 gene. We established that there is a single mouse T beta 4 gene and found that the lymphoid-specific transcript is generated by extending the ubiquitous exon 1 with an alternate downstream splice site. The transcription start site is defined by primer extension analysis, and the 5'-flanking region has many of the characteristics of a promoter. It is pyrimidine-rich and contains typical promoter elements, including a GC box, an initiator site, and consensus transcription factor binding sites. The mouse T beta 4 gene locus (Ptmb4) is located by interspecific backcross mapping to the distal region of the mouse X chromosome, linked to Btk and Gja6.
Li
X
X
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040, USA.
Zimmerman
A
A
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Yin
H L
HL
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005796:Genes / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013947:Thymosin / Q000235:genetics*; D014960:X Chromosome; D000099201:Thymosin beta(4)
D012333:RNA, Messenger; D013947:Thymosin; D000099201:Thymosin beta(4)