title: Transcription of mutS and mutL-homologous genes in Saccharomyces cerevisiae during the cell cycle.
abstract: Transcription of the Saccharomyces cerevisiae DNA mismatch repair genes PMS1, MSH2, and MSH6, a recently discovered homolog of the Escherichia coli mutS gene, was shown to be cell cycle regulated. In contrast, transcription of the MSH1, MSH3 and MLH1 genes was not regulated during the cell cycle. The MSH1 gene, which is thought to be involved in DNA mismatch repair in mitochondria, was also not induced under aerobic growth conditions. Regulation of the PMS1 gene was dependent on intact MluI cell cycle boxes, as demonstrated by analysis of a promoter mutant. Both reduced and increased expression of PMS1 resulted in a mitotic mutator phenotype. Analysis of mRNA levels was performed with a newly developed reverse transcription-PCR (polymerase chain reaction) approach using fluorescently labeled primers and an automated DNA sequencer for detection of PCR products.
authors.affiliation: Institut für Molekulare Genetik, Georg-August-Universität, Göttingen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kramer
authors.forename: B
authors.initials: B
authors.lastname: Fartmann
authors.forename: E C
authors.initials: EC
authors.lastname: Ringbeck
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000251:Adenosine Triphosphatases; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins; D002453:Cell Cycle / Q000235:genetics; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D024101:Mitochondrial Proteins; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; D051718:MutS Homolog 2 Protein; D000074081:MutS Homolog 3 Protein; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; C088859:MLH1 protein, S cerevisiae; C078660:MSH1 protein, S cerevisiae; C081696:MSH3 protein, S cerevisiae; C098790:MSH6 protein, S cerevisiae; D024101:Mitochondrial Proteins; C074770:MutL protein, E coli; D000074081:MutS Homolog 3 Protein; C088858:PMS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C489751:MSH2 protein, S cerevisiae; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; D051718:MutS Homolog 2 Protein; C497175:MutS protein, E coli