Human T lymphocyte cell line (Mo) and its subclone (J) produce colony stimulating activity on normal and malignant T cell precursors.
Conditioned medium from a T-lymphoblastic cell line (Mo) is known to produce factors promoting CFU-GM, BFU-E and CFU-MK. In our study we investigated the potential CSA of conditioned media obtained from Mo and its subclone J on normal and malignant lymphoid progenitors of both T and B lineage. Both cell lines release factors inducing a significant increase in number and size of T-lymphoid colonies when compared to standard source of factors (PHA-LCM). On the contrary, they presented a low CSA on B cell precursors confirming the difficulties in identifying a source of growth and differentiation factors for human B cell ontogeny. This study contributes to the knowledege of biological properties of these tumor cell lines, suggesting the possibility to employ Mo- and J-derived supernatants in vitro for improving growth potential of normal and malignant T cell progenitors.
Legitimo
A
A
Clinical Pediatrical Institute, University of Pisa, Italy.
Consolini
R
R
Bason
C
C
Balleari
E
E
Macchia
P
P
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D014411:Neoplastic Stem Cells; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000473:pathology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D016207:Cytokines