Distinction of species in Aureobasidium and related genera by PCR-ribotyping.
Taxonomic markers for differentiation of the anamorph genera Aureobasidium, Hormonema and Kabatiella were developed using PCR-ribotyping with the primers 5.8S-R and LR7 for amplification and the restriction enzymes Alul, Ddel, Hhal, Mspl and Rsal for digestion. Aureobasidium and Hormonema are optimally differentiated with Mspl; Ddel is particularly useful to distinguish Aureobasidium, Kabatiella and Selenophoma. Relationships of the anamorph genera Aureobasidium, Hormonema and Kabatiella with the teleomorph genera Pringsheimia and Dothiora are discussed.
Yurlova
N A
NA
Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.
Uijthof
J M
JM
de Hoog
G S
GS
D002965:Classification; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004275:DNA, Ribosomal / Q000145:classification / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000235:genetics*; D005819:Genetic Markers; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D004275:DNA, Ribosomal; D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes