title: Monoclonal antibodies against fimbrial subunits of colonization factor antigen I (CFA/I) inhibit binding to human enterocytes and protect against enterotoxigenic Escherichia coli expressing heterologous colonization factors.
abstract: Enterotoxigenic Escherichia coli (ETEC) bind to enterocytes in the small intestine by means of antigenically distinct colonization factors (CFs). By immunizing with isolated subunits of CFA/I fimbriae we have previously produced monoclonal antibodies (MAbs) that cross-react immunologically in vitro with several CFs. Two of these MAbs [S(subunit)-CFA/ I 17:8 and S-CFA/I 5:6] were found to significantly inhibit the binding of ETEC strains expressing either homologous or heterologous CFs, i.e. CFA/I and CS4, to isolated human jejunal enterocytes. The two MAbs also conferred passive protection against fluid accumulation in rabbit ileal loops caused by CFA/I-as well as CS4-expressing ETEC strains. Immunoelectron microscopy studies showed that both MAbs bound specifically to CFA/I as well as to CS4 fimbriae expressed on bacteria. These results indicate the possibility to induce anti-CF antibodies that can protect against ETEC infection caused by bacteria expressing not only homologous but also heterologous CFs, by immunizing with fimbrial subunits.
authors.affiliation: Department of Medical Microbiology and Immunology, Göteborg University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Rudin
authors.forename: L
authors.initials: L
authors.lastname: Olbe
authors.forename: A M
authors.initials: AM
authors.lastname: Svennerholm
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001422:Bacterial Adhesion / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control*; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D006801:Humans; D007082:Ileum / Q000382:microbiology; D007116:Immunization, Passive; D007583:Jejunum / Q000166:cytology; D016253:Microscopy, Immunoelectron; D011817:Rabbits
chemical_list: D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal