title: C-terminal deletions can suppress temperature-sensitive mutations and change dominance in the phage Mu repressor.
abstract: Mutations in an N-terminal 70-amino acid domain of bacteriophage Mu's repressor cause temperature-sensitive DNA-binding activity. Surprisingly, amber mutations can conditionally correct the heat-sensitive defect in three mutant forms of the repressor gene, cts25 (D43-G), cts62 (R47-Q) and cts71 (M28-I), and in the appropriate bacterial host produce a heat-stable Sts phenotype (for survival of temperature shifts). Sts repressor mutants are heat sensitive when in supE or supF hosts and heat resistant when in Sup degrees hosts. Mutants with an Sts phenotype have amber mutations at one of three codons, Q179, Q187, or Q190. The Sts phenotype relates to the repressor size: in Sup degrees hosts sts repressors are shorter by seven, 10, or 18 amino acids compared to repressors in supE or supF hosts. The truncated form of the sts62-1 repressor, which lacks 18 residues (Q179-V196), binds Mu operator DNA more stably at 42 degrees in vitro compared to its full-length counterpart (cts62 repressor). In addition to influencing temperature sensitivity, the C-terminus appears to control the susceptibility to in vivo Clp proteolysis by influencing the multimeric structure of repressor.
authors.affiliation: Department of Biochemistry, University of Alabama, Birmingham 35294, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Vogel
authors.forename: V
authors.initials: V
authors.lastname: Geuskens
authors.forename: L
authors.initials: L
authors.lastname: Desmet
authors.forename: N P
authors.initials: NP
authors.lastname: Higgins
authors.forename: A
authors.initials: A
authors.lastname: Toussaint
mesh_terms: D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D010583:Bacteriophage mu / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004279:DNA, Viral; D049071:Endopeptidase Clp; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D013697:Thermosensing; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D004279:DNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases