title: Complete nucleotide sequence and biological properties of an infectious clone of prototype echovirus 9.
abstract: The prototype Hill of echovirus 9, a human enterovirus, exhibits no pathogenicity for newborn mice in contrast to some other echovirus 9 strains isolated subsequently during epidemics. In this communication we report the first complete nucleotide sequence and construction of an infectious clone of echovirus 9. Aside from the 3' poly(A)-tract, the RNA genome is 7420 nucleotides (nt) in length and encodes a single polyprotein of 2193 amino acids (aa). The open reading frame extends from position 740 to position 7318 of the genome. Sequence comparisons to other enteroviruses reveal a strong overall amino acid identity to echovirus types 11 and 12 (in each case 76%). The proteolytic cleavage sites of the three major capsid proteins were determined after purification by HPLC and protein sequencing. A full-length clone coding for an infectious RNA transcript was constructed, and recombinant echovirus 9 particles could be isolated from the supernatant of transfected cell culture. It is shown that the recombinant virus, like the original prototype, is non-pathogenic for newborn mice and does not multiply in skeletal muscles.
authors.affiliation: Institut fuer Virologie der Universitaet zu Koeln, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Zimmermann
authors.forename: H J
authors.initials: HJ
authors.lastname: Eggers
authors.forename: A
authors.initials: A
authors.lastname: Zimmermann
authors.forename: W
authors.initials: W
authors.lastname: Kraus
authors.forename: B
authors.initials: B
authors.lastname: Nelsen-Salz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004459:Echovirus 9 / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral
chemical_list: D012367:RNA, Viral