The protective effect of allopurinol on cholestatic liver injury induced by bile duct ligation.
To determine whether oxygen free radicals are responsible for the pathogenesis of the cholestasis induced by ligation of common bile duct (CBD) variables which reflect the hepatic function in the serum, the amount of superoxide radical production, and xanthine oxidase(XO) activity were studied. The activity of serum alanine aminotransferase, bilirubin level in the serum and the amount of superoxide radical production were lower in a CBD ligation with allopurinol treated group than in a CBD ligation without allopurinol treated group. Abnormalities of the microscopic structures were reduced in a CBD ligation with allopurinol treated group than in a CBD ligation without allopurinol treated group. Allopurinol, an inhibitor of XO, prevented the hepatic damage induced by CBD ligation through the inhibition of XO. These experiments demonstrate that oxygen free radicals are responsible for the pathogenesis of the cholestatic liver.
Mun
K C
KC
Department of Biochemistry and Pathology, Keimyung University School of Medicine, Taegu, Korea.
Kwak
C S
CS
Kwon
K Y
KY
D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D001652:Bile Ducts; D002779:Cholestasis / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005609:Free Radicals; D008026:Ligation; D008099:Liver / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000032:analysis / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D005609:Free Radicals; D013481:Superoxides; D000493:Allopurinol; D014969:Xanthine Oxidase