Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose.
Fluorescence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.
Schwinefus
J J
JJ
University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
Wang
S C
SC
Hammond
R W
RW
Morris
M D
MD
D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D004586:Electrophoresis / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D017900:Myoviridae / Q000235:genetics; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence
D004279:DNA, Viral; D002482:Cellulose; C002283:hydroxyethylcellulose