Polymorphism of erythrocyte acid phosphatase system (ACP, EC 3.1.3.2) in hemolysates and bloodstains. I. Evaluation of selected methods of ACP phenotyping.
A specific ampholine mixture resulted in appreciable enhancement of discriminating features in electrophoregrams of 6 common ACP phenotypes, thus allowing unequivocal interpretation of the enzyme types in fresh blood and in bloodstains. The employed procedure resulted in marked increase in the limit of ACP phenotype detectability in bloodstains and substantial enhancement of sensitivity and efficacy of the analysis. PhastIEF appeared useful in ACP phenotyping due to its reproducibility and duration of separation. In the case of some ACP phenotypes in ageing bloodstains this technique allows even longer detectability limits compared to PAGE IEF.
Skawrońska
M
M
Department of Forensic Medicine, Medical Academy of Białystok.
Janica
J
J
Pepiński
W
W
Nałecka
M
M
D000135:Acid Phosphatase / Q000032:analysis* / Q000235:genetics; D001801:Blood Stains; D004586:Electrophoresis / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D006461:Hemolysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
D007527:Isoenzymes; D000135:Acid Phosphatase