Perinatal lung carcinogenesis in mice: in vivo and in vitro correlation.
Perinatal lung carcinogenesis in susceptible (A) and resistant (C57B1/6J) to lung carcinogenesis mice was studied in (1) experiment in vivo and (2) in organ cultures of embryonic lung exposed to carcinogens directly (in vitro) or transplacentally (in utero): females were treated with carcinogens at 17-19 days of pregnancy, killed 1-2 days after the treatment and embryonic lungs were explanted in organ cultures. Transplacental and direct (in vitro) treatment with urethane and benz(a)pyrene (BP) induced in the explants: (1) stimulation of epithelial and mesenchymal cell proliferation; (2) increase of viability and survival as compared to the non-treated explants (growth stimulating effect); (3) morphological lesions (hyperplasia and squamous-cell metaplasia of epithelium, adenomatous growth and adenoma-like structures). All the alterations developed in treated lung explants depended both on host factors and carcinogenic treatment (type of carcinogen, dose, method of exposure, and duration of cultivation).
Turusov
V S
VS
Cancer Research Centre, Russian Academy of Medical Sciences, Moscow.
Kolesnichenko
T S
TS
Gor'kova
T G
TG
D000818:Animals; D000831:Animals, Newborn; D015197:Carcinogenicity Tests; D002455:Cell Division / Q000187:drug effects; D004198:Disease Susceptibility; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000196:embryology / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000196:embryology / Q000473:pathology; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D011247:Pregnancy; D014022:Tissue Survival / Q000187:drug effects