title: A biochemical and immunological comparison of nuclear and cytoplasmic pore complexes.
abstract: Pore complexes are not confined to the nuclear envelope but can also be found in the cytoplasm of numerous cell types in the form of annulate lamellae (AL). We have induced formation of AL by exposure of rat cells (line RV) to sublethal doses of the antimitotic drug vinblastine sulfate, and compared the distribution of several nuclear pore complex proteins (nucleoporins) in the nuclear envelope and AL by immunocytochemistry, cytochemical lectin binding studies and immunoblot analyses of nuclear and AL-enriched fractions. All the antibodies used yielded punctate nuclear surface staining in immunofluorescence microscopy which is characteristic for nuclear pore complex components. When we applied antibodies against the nucleoporin p62, AL were visualized as numerous cytoplasmic dot-like structures. Immunogold electron microscopy confirmed the correspondence of the cytoplasmic bodies with stacks of AL. Antibodies to constituents of the cytoplasmic (nup180) and nucleoplasmic (nup153) filaments extending from both sides of nuclear pore complexes also stained the AL, indicating that pore complexes are intrinsically asymmetric assemblies independent of their specific intracellular topology. By contrast, AL were negative with five different antibodies against the transmembrane nuclear pore glycoprotein gp210 and the lectin concanavalin A (ConA) known to bind to the oligosaccharide side chains of gp210. Similarly, there was no staining of the AL with antibodies to the other nuclear pore membrane protein so far known in higher eukaryotes, POM121. Immunoblot analyses confirmed the presence of p62, nup180 and nup153 in both the nuclear and AL fractions and the absence of gp210 and POM121 from AL. Our results do not support the generally held view that gp210 and POM121 function in anchoring the pore complex scaffold to the pore membrane. Rather, they point to a role for these proteins in transport processes through the nuclear pore complexes. Since AL are not involved in nucleocytoplasmic transport processes they may lack components of the transport machinery.
authors.affiliation: Department of Cell and Developmental Biology, Theodor-Boveri-Institute, University of Würzburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ewald
authors.forename: U
authors.initials: U
authors.lastname: Kossner
authors.forename: U
authors.initials: U
authors.lastname: Scheer
authors.forename: M C
authors.initials: MC
authors.lastname: Dabauvalle
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure*; D018272:Porins / Q000032:analysis*; D051381:Rats
chemical_list: D018272:Porins