Determination of the pharmaceutical peptide TP9201 by post-column reaction with copper(II) followed by electrochemical detection.
An electrochemical detection method was applied to the determination of the synthetic peptide TP9201 (Telios Pharmaceuticals). The method utilizes reversed-phase HPLC, followed by post-column formation of Cu(II)-peptide complexes to render peptides electrochemically active via the Cu(III/II) couple. TP9201 is cyclic and N-amidated; the lack of a free amine precludes the use of typical fluorescent labeling reagents. Neither the cyclic structure nor the N-amidation prevented the copper complexation reaction, however. The detection limit in bovine serum was 20 nM, limited by interfering sample peaks, and the detector response was linear in a range 10-400 nM.
Woltman
S J
SJ
Chevron Science Center, University of Pittsburgh, PA 15260, USA.
Chen
J G
JG
Weber
S G
SG
Tolley
J O
JO
D000595:Amino Acid Sequence; D000818:Animals; D001737:Biuret; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D004563:Electrochemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010456:Peptides, Cyclic; D003300:Copper; C085362:TP 9201; D001737:Biuret