title: Using PCR for rapid site-specific mutagenesis in large plasmids.
authors.affiliation: Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Watkins
authors.forename: M S
authors.initials: MS
authors.lastname: Reitz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic; D014169:Transformation, Bacterial
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant