Effect of haloallylamines on polyamine oxidase activity and spermine levels in Ascaris suum.
In parasitic nematodes the rate-limiting step in the polyamine interconversion pathway is catalysed by polyamine oxidase. MDL 72527, the specific inhibitor of mammalian polyamine oxidase, had no effect on the Ascaris suum enzyme, whereas its activity was inhibited in a time-dependent manner by the haloallylamine MDL 72145, originally designed as a specific inhibitor of monoamine oxidase A and B. The dissociation constant (Ki) was found to be 0.9 microM and the enzyme half-life under saturation conditions (t50) was determined to be 0.8 min. Incubation of A. suum in vitro in the presence of 50 microM MDL 72145 for 6 h resulted in a decrease in polyamine oxidase activity to about 20% of the control value, and spermine concentrations simultaneously increased about 200%. Both results suggest that MDL 72145 might be a chemical lead compound for the design of new chemotherapeutic agents against nematode infections.
Müller
S
S
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Hunter
K J
KJ
Walter
R D
RD
D000499:Allylamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D017165:Ascaris suum / Q000201:enzymology*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011700:Putrescine / Q000031:analogs & derivatives / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism*; D000097667:Polyamine Oxidase
D000499:Allylamine; D000969:Antinematodal Agents; D008996:Monoamine Oxidase Inhibitors; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011700:Putrescine; D013096:Spermine; D000097667:Polyamine Oxidase; C044445:MDL 72527; C040051:2-(3,4-dimethoxyphenyl)-3-fluoroallylamine