title: Evaluation of cholecystokinin, gastrin, CCK-A receptor, and CCK-B/gastrin receptor gene expressions in gastric cancer.
abstract: The brain-gut hormones, cholecystokinin (CCK) and gastrin, regulate the growth of gastrointestinal mucosa and tumor cells. In this study, reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate messenger RNA expression for CCK, gastrin, CCK-A receptor, and CCK-B/gastrin receptor in surgical specimens of gastric cancers and in normal antrum and body mucosa of the stomach. The CCK mRNA expression was detectable in 4/14 (29%) samples of gastric cancer and in 3/12 (25%) samples of antral mucosa. However, the gastrin mRNA expression was not detectable in any gastric cancer samples, although it was detectable in all the samples of antral mucosa. The CCK-A receptor mRNA expression was detectable in 5/14 (36%) samples of gastric cancer and in 7/12 (58%) body mucosa. Three cases out of 14 (21%) of gastric cancer expressed both CCK gene and CCK-A receptor gene. The CCK-B receptor mRNA expression was detectable in only 1/14 (7%) samples of gastric cancer, although it was detectable in 10/12 (83%) body mucosa of the stomach. These findings may suggest a greater role for CCK and CCK-A receptor than for gastrin and CCK-B receptor in gastric cancers.
authors.affiliation: First Department of Surgery, Faculty of Medicine, Kyoto University, Japan. okada@metab.kuhp.kyoto-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Okada
authors.forename: A
authors.initials: A
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Imamura
authors.forename: H
authors.initials: H
authors.lastname: Suwa
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: G
authors.initials: G
authors.lastname: Ohshio
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
authors.forename: M
authors.initials: M
authors.lastname: Imamura
mesh_terms: D002766:Cholecystokinin / Q000032:analysis / Q000235:genetics*; D005755:Gastrins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D011863:Radioimmunoassay; D011949:Receptors, Cholecystokinin / Q000235:genetics*; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D005755:Gastrins; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin