Quantitative assessment of murine retrovirus LP-BM5 infection in MAIDS by PCR and anion exchange HPLC.
In vitro amplification of DNA by PCR is a powerful tool to detect small amounts of DNA. It is now widely used for detection of pathogenic agents from extracellular fluids and organs. The use of anion exchange HPLC to quantify the PCR product resulting from the specific amplification of the DNA from the replicative-defective viral DNA responsible for MAIDS is described. This technique allows precise quantification of MAIDS virus DNA in different organs and circumvents the use of radioactivity and gel electrophoresis.
Hulier
E
E
INSERM U313, Department of Parasitology, CHU Pitié-Salpètrière, Paris, France.
Pétour
P
P
Marussig
M
M
Nivez
M P
MP
Mazier
D
D
Rénia
L
L
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification*; D008198:Lymph Nodes / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004279:DNA, Viral