Eliminant effects of calcitonin gene related peptide on the tubular epithelial cells in an isolated and perfused rat kidney.
The effect of the infusion of calcitonin gene related peptide (CGRP) on the renal structure and enzyme release from tubular epithelial cells was studied. This study was performed in the model of an isolated perfused kidney to avoid the systemic effects and complex hormonal and neuromediated interaction following the CGRP infusion in the intact experimental animal. After infusion of CGRP, the perfused kidney, studied by semiquantitative histology and electron microscopy, did not show any alteration at the glomerular level; however, important histological lesions were apparent at the proximal tubular level: the brush border was destroyed and the epithelial cells were markedly flattened. This structural damage to epithelial cells was confirmed by electron microscopy. The alanine amino peptidase (AAP) and N-acetyl-glucosaminidase (NAG) were significantly increased in the effluent of the perfusion system, in confirmation of the histological damage. The direct toxic effect of CGRP on the tubular epithelial cells may be related to the reabsorption and tubular transport of this substance.
Giovannini
L
L
Institute of Pharmacology, University of Pisa, Italy.
Palla
R
R
Bertelli
A A
AA
Torri Tarelli
L
L
Tazzari
S
S
Migliori
M
M
Scalori
V
V
Panichi
V
V
Parrini
M
M
D000118:Acetylglucosaminidase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D018826:CD13 Antigens / Q000187:drug effects / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D000118:Acetylglucosaminidase; D018826:CD13 Antigens; D015740:Calcitonin Gene-Related Peptide