Direct determination of selenium in human blood serum and plasma by electrothermal atomic absorption spectrometry.
A method is described for the direct determination of selenium in serum or plasma by electrothermal atomic absorption spectrometry with deuterium-arc background correction. Samples are diluted (1 + 2) with a modifier containing palladium nitrate and Triton X-100. Samples are atomised from a L'vov platform in a pyrolytically-coated electrographite tube and peak area signals are measured. Direct determination is possible by using selenium standards matched to the physiological concentrations of sodium chloride, calcium and phosphate. The detection limit is 6 micrograms/L in the original sample. Precision at a selenium concentration of 97 micrograms/L was 2.2% RSD within batch and 3.0% RSD between batch. Accuracy is shown by (i) analysis of a Seronorm reference serum (value obtained 97 +/- 3 micrograms/L; recommended value 96 micrograms/L); (ii) recovery of added selenium (93.3 +/- 6.7% and 98.2 +/- 3.3% at additions of 30 and 60 micrograms/L, respectively) and (iii) comparison of results with mean of all laboratories in an external quality assessment scheme.
Gardiner
P H
PH
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Littlejohn
D
D
Halls
D J
DJ
Fell
G S
GS
D002138:Calibration; D003903:Deuterium / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012643:Selenium / Q000097:blood*; D013054:Spectrophotometry, Atomic / Q000379:methods*
D010710:Phosphates; D010165:Palladium; D003903:Deuterium; D007501:Iron; D012643:Selenium