Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization.
We have isolated a 3.3-kb DNA containing the two exons and a 2.6-kb intron of the human CPX2 gene. This gene encodes the intestinal isoenzyme of glutathione peroxidase, GSHPx-GI. Consistent signals were detected at 14q24.1 when this DNA was used as a probe for fluorescence in situ hybridization on metaphase chromosomes. Based on either single-stranded conformation polymorphism or sequencing analysis, two sites containing DNA polymorphism were found in the intron: the 5' end had a single A/T alteration, and the 3' end had a microsatellite of TC repeats.
Chu
F F
FF
Department of Medical Oncology & Therapeutics Research, City of Hope Medical Center, Duarte, CA 91010, USA. fchu@smtplink.coh.org
Rohan de Silva
H A
HA
Esworthy
R S
RS
Boteva
K K
KK
Walters
C E
CE
Roses
A
A
Rao
P N
PN
Pettenati
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004247:DNA; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational
D007527:Isoenzymes; D004247:DNA; D005979:Glutathione Peroxidase