Nuclear proteins bind to poliovirus 5' untranslated region.
Poliovirus induces a shut-off of messenger RNA (mRNA) translation by the proteolysis of a 220 kDa protein from the eukaryotic initiation factor eIF-4F, and by the phosphorylation of eIF-2. The absence of eIF-4F inhibits the initiation of translation dependent on cap structure recognition. Poliovirus RNA lacks cap structure and translates by a cap-independent mechanism which requires internal ribosomal entry. The poliovirus 5' untranslated region (5'UTR) contains the structural elements for cap-independent translation called internal ribosomal entry site (IRES element). Several cellular proteins have been described interacting with different segments from poliovirus 5'UTR. We have studied the specific interaction between 57/60, 52, and 35 kDa cellular proteins with poliovirus nt 275 to 636 and full length 5'UTR. By Western blot assay the 57/60 protein was identified as a pyrimidine tract binding protein (PTB), and the 52 kDa protein as a La-autoantigen. La and PTB are nuclear proteins involved in RNA polymerase III transcription termination and splicing, respectively.
Gutiérrez-Escolano
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Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
del Angel
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RM
D001324:Autoantigens / Q000378:metabolism*; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D039562:Eukaryotic Initiation Factor-4F; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000037:antagonists & inhibitors; D017955:Poliovirus / Q000235:genetics* / Q000502:physiology; D038941:Polypyrimidine Tract-Binding Protein; D014176:Protein Biosynthesis; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012261:Ribonucleoproteins / Q000378:metabolism*; D000097585:SS-B Antigen
D001324:Autoantigens; D004268:DNA-Binding Proteins; D039562:Eukaryotic Initiation Factor-4F; D009687:Nuclear Proteins; D010448:Peptide Initiation Factors; D038941:Polypyrimidine Tract-Binding Protein; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen