Alkali cation transport through liposomes by the antimicrobial fusafungine and its constitutive enniatins.
Fusafungine is a peptide antibiotic mixture composed of several enniatins and active against Gram-positive bacteria. Ionophoric properties of fusafungine have been studied in liposomes by measuring protoncation exchange by both fluorescence and 31P-nuclear magnetic resonance (NMR) and have been compared to those of its constituent enniatin peptides. Fusafungine, as well as enniatins, transport cations through a mobile carrier mechanism selective for K+ vs. Na+ and involving two antibiotic molecules. The transport efficiencies of the various enniatins appear to be related to their hydrophobicity, in agreement with a previously proposed "sandwich" transport model. The ionophoric properties of crude fusafungine may be involved in its antibiotic action and its local therapeutic properties.
Levy
D
D
Laboratoire de Biophysique Cellulaire, CNRS-URA 526, Université Paris 7, France.
Bluzat
A
A
Seigneuret
M
M
Rigaud
J L
JL
D000336:Aerosols / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001190:Arylsulfonates; D001692:Biological Transport / Q000187:drug effects; D002412:Cations / Q000737:chemistry*; D047630:Depsipeptides; D005456:Fluorescent Dyes; D005670:Fusarium; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides; D010710:Phosphates / Q000032:analysis
D000336:Aerosols; D000900:Anti-Bacterial Agents; D001190:Arylsulfonates; D002412:Cations; D047630:Depsipeptides; D005456:Fluorescent Dyes; D008081:Liposomes; D010455:Peptides; D010710:Phosphates; C100264:enniatins; C014001:fusafungin; C005047:pyranine