[Diagnostic microbiology of DNA in septicemia].
Bacterial species encode rRNAs that are functionally and evolutionarily conserved. The nucleotide sequences of bacterial 16S ribosomal RNAs (16S rRNAs) diverge in regions of around 1,400 bases. Bacterial 16S rRNAs possess nine or more conserved "universal" sequences. Between each conserved region there are species specific sequences. These regions provide targets for clinical detection and diagnoses based on molecular hybridization for the polymerase chain reaction. We designed one base discriminating primer in 16S rRNA for the specific amplification of these regions and the primers in 16S-23S spacer regions of the rrn operon. These DNA-amplification based diagnosis of the septicemia showed multiple bacterial infection and indicated uncommon species of bacteria in the blood.
Hoshina
S
S
Department of Laboratory Medicine, Jikei University School of Medicine, Tokyo.
Machida
K
K
D016470:Bacteremia / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S