A new method for removal of the 2'-TBDMS group under acidic conditions in chemical synthesis of RNA.
A new and convenient method for removal of the TBDMS group as a protecting group for the 2' hydroxyl function in chemical synthesis of RNA under mild acidic conditions by use of diluted acetic acid solutions and 0.01 M hydrochloric acid (pH 2.0) was developed.
Kawahara
S
S
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Wada
T
T
Sekine
M
M
D019342:Acetic Acid; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D000644:Quaternary Ammonium Compounds; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D012313:RNA; C009405:tetrabutylammonium; D006152:Cyclic GMP; D019342:Acetic Acid