Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium.
mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcript abundance over time in soil-grown P. chrysosporium varied among the nine lip mRNAs detected; comparison with lip gene expression under different liquid culture conditions suggested an early phase of carbon limitation for the cultures as a whole, which was followed by a transition to nitrogen starvation. Anthracene transformation occurred throughout the 25-day course of the experiment and, therefore, likely involves mechanisms distinct from those involved in oxidation of non-LiP substrate polycyclic aromatic hydrocarbons.
Bogan
B W
BW
Department of Cell and Molecular Biology, University of Wisconsin-Madison 53706, USA.
Schoenike
B
B
Lamar
R T
RT
Cullen
D
D
D000873:Anthracenes / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013997:Time Factors
D000873:Anthracenes; D000880:Anthraquinones; D012331:RNA, Fungal; D012333:RNA, Messenger; D012989:Soil Pollutants; C042834:9,10-anthraquinone; D010544:Peroxidases; C042858:lignin peroxidase; C034020:anthracene