title: Human T-lymphotropic virus type I (HTLV-I)-specific antibodies and cell-free RNA in crevicular fluid-rich saliva from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy.
abstract: Despite the likely role of mucosae in human T cell leukemia virus type I (HTLV-I) transmission, little is known about the mucosal immune response to HTLV-I. The present study evaluated the antibody response to HTLV-I in oral mucosa and the value of crevicular fluid rich saliva (CFRS) for diagnosing HTLV-I infection. CFRS and sera from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM), asymptomatic carriers, and HTLV-I seronegative individuals from Tumaco, Colombia, were analyzed for HTLV-I specific IgG, IgA, and secretory IgA (sIgA). Detection of IgG in CFRS by enzyme-linked immunosorbent assay correlated with its presence in sera for TSP/HAM patients and asymptomatic carriers. IgA and sIgA were more frequently detected in CFRS and sera from TSP/HAM patients than in those from asymptomatic carriers. An HTLV-I pol fragment could be amplified from CFRS by reverse transcriptase-PCR in 3 TSP/HAM patients and one asymptomatic carrier, all of whom had an IgA response in CFRS but not in sera. The more frequent detection of IgA and sIgA in sera and CFRS of TSP/HAM patients suggests increased viral replication. Further, the association of viral RNA in CFRS with a local IgA response may signify rounds of viral replication in the oral cavity.
authors.affiliation: Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
authors.forename: L E
authors.initials: LE
authors.lastname: Soto-Ramirez
authors.forename: F
authors.initials: F
authors.lastname: Garcia-Vallejo
authors.forename: B
authors.initials: B
authors.lastname: Renjifo
authors.forename: A
authors.initials: A
authors.lastname: Vergara
authors.forename: I
authors.initials: I
authors.lastname: Borrero
authors.forename: R
authors.initials: R
authors.lastname: Marlink
authors.forename: M
authors.initials: M
authors.lastname: Essex
mesh_terms: D000918:Antibody Specificity / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000276:immunology* / Q000821:virology; D015481:HTLV-I Antibodies / Q000032:analysis* / Q000097:blood; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D015493:Paraparesis, Tropical Spastic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D012463:Saliva / Q000276:immunology* / Q000821:virology; D013295:Streptococcus mutans / Q000276:immunology
chemical_list: D015481:HTLV-I Antibodies; D012367:RNA, Viral