Immunomodulative effects of aflatoxins and selenium on human peripheral blood lymphocytes.
We investigated the antagonist immunomodulative properties of aflatoxin B1 and selenium on human peripheral blood lymphocytes by 2 colorimetric in vitro cytotoxicity assays, the neutral red uptake and the thiazolyl blue tetrazolium bromide assay. Immunotoxic properties of these substances were determined by a (3H)-thymidine DNA incorporation assay on phytohemagglutinin (PHA) stimulated and non-stimulated lymphocytes. Aflatoxin B1 and selenium, when studied separately, had an immunosuppressive effect on PHA stimulated and non-stimulated human peripheral blood lymphocytes. When they were studied together, they had an antagonistic effect on this immunological parameter.
Methenitou
G
G
Department of Forensic Medicine and Toxicology, School of Medicine, Athens, Greece.
Maravelias
C
C
Koutsogeorgopoulou
L
L
Athanaselis
S
S
Koutselinis
A
A
D000276:Adjuvants, Immunologic / Q000633:toxicity*; D016604:Aflatoxin B1 / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D003124:Colorimetry; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004347:Drug Interactions; D006801:Humans; D007166:Immunosuppressive Agents / Q000633:toxicity; D007553:Isotope Labeling; D009499:Neutral Red / Q000737:chemistry / Q000378:metabolism; D010835:Phytohemagglutinins / Q000633:toxicity; D012643:Selenium / Q000633:toxicity*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000276:Adjuvants, Immunologic; D002273:Carcinogens; D004396:Coloring Agents; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; D009499:Neutral Red; D004247:DNA; D016604:Aflatoxin B1; C022616:thiazolyl blue; D012643:Selenium; D013936:Thymidine