Excimer formation by hybridization using two pyrene-labeled oligonucleotide probes.
Pyrene excimer formation was achieved in an aqueous solution by nucleic acid hybridization. We used two different 16-mer oligonucleotide probes which had a combined continuous-sequence run complimentary to a target 32-mer. Prior to hybridization, the adjacent terminal ends (i.e., the 3'-terminal of one probe and the 5'-terminal of the other probe) were each labeled with one pyrene residue. The simultaneous hybridization of the probes to the target induced a 495-nm broad fluorescence band characteristic for pyrene excimer. Temperature and molecular configurations of the hybrid influenced the excimer formation.
Ebata
K
K
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Masuko
M
M
Ohtani
H
H
Jibu
M
M
D001483:Base Sequence; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D012996:Solutions; D013053:Spectrophotometry; D013696:Temperature; D014867:Water
D015345:Oligonucleotide Probes; D011721:Pyrenes; D012996:Solutions; D014867:Water; C030984:pyrene