Hypoosmolarity and glutamine increased the beta-actin gene transcription in isolated rat hepatocytes.
The mechanism of action of hydration state was studied on beta-actin gene expression in isolated hepatocytes. Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the beta-actin mRNA level through a corresponding modulation of the rate of the gene transcription. Glutamine, which is known to induce cell swelling, also increased the beta-actin mRNA level in a dose-dependent manner and induced a stimulation of the beta-actin gene transcription. Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.
Husson
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Institut Fédératif de Recherches Multidisciplinaires sur les PeptidesNo. 23 (IFRMP), U.F.R. Médecine-Pharmacie de Rouen, Saint-Etienne-du-Rouvray Cedex, France.
Quillard
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M
Fairand
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Chedeville
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Lavoinne
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D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D048429:Cell Size; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D005973:Glutamine / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D019384:Nucleic Acid Synthesis Inhibitors; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic; D014867:Water / Q000378:metabolism
D000199:Actins; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D014867:Water; D005973:Glutamine; D003609:Dactinomycin