Involvement of two types of TNF receptor in TNF-alpha induced neutrophil apoptosis.
We previously demonstrated that tumor necrosis factor-alpha (TNF-alpha) induces rapid human neutrophil apoptosis. In this paper, we examined which of the TNF receptors, p55 kDa TNF receptor (55-R) or p75 kDa TNF receptor (75-R), or both are involved in this process using specific rabbit antisera. Antibodies to 55-R (anti55-R) or 75-R (anti75-R) alone did not induce neutrophil apoptosis. Further addition of cycloheximide and anti-rabbit immunoglobulin to anti55-R but not to anti75-R accelerated apoptosis, although anti75-R augmented the capacity of anti55-R to do so. These results suggest that 55-R is a prerequisite for TNF-alpha induced neutrophil apoptosis.
Gon
S
S
Department of Immunology and Parasitology, Yamagata University School of Medicine, Japan.
Gatanaga
T
T
Sendo
F
F
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology*; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000494:pharmacology
D015703:Antigens, CD; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha