Differential expression of the mRNAs of the axonal glycoproteins axonin-1 and NgCAM in the developing chick retina.
Cell adhesion molecules expressed on the axonal membrane have been thought to be involved in the guidance of axons to their target area. In the chick, axonin-1 and NgCAM have been shown to promote, through reciprocal interactions, neurite outgrowth in vitro. We have recently shown that chick retinal ganglion cells (RGC) express both proteins as early as the axonal elongation begins. Their expression continues throughout the development of the retinotectal system synchronously with the chronotopic spread of axons. To further investigate the spatiotemporal distribution of axonin-1 and NgCAM in the retina, we have analysed the expression of their mRNAs in the present study. From stage 36 (E10) until hatching photoreceptors express axonin-1 but not NgCAM. In the inner nuclear layer groups of amacrine cells were strongly labelled with both probes but they seemed to belong to different subgroups. These patterns of expression might indicate a differential influence of the two proteins on the development of the local neural circuits of the retina.
Morino
P
P
Institute of Anatomy, University of Fribourg, Switzerland.
Buchstaller
A
A
Giger
R
R
Sonderegger
P
P
Rager
G
G
D000818:Animals; D001369:Axons / Q000502:physiology*; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002642:Chick Embryo; D058969:Contactin 2; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017403:In Situ Hybridization; D007395:Interneurons / Q000502:physiology; D010786:Photoreceptor Cells / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000502:physiology*
C548674:CNTN2 protein, human; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D058969:Contactin 2; C491152:NRCAM protein, human; D012333:RNA, Messenger