Liposomal ATP protects the liver from injury during shock.
Liposomes are useful drug carriers. In this study, adenosine triphosphate (ATP) was entrapped in liposomes (Lip-ATP). Anesthetized rats were given Lip-ATP (5 mg/kg as ATP), free ATP (5 mg/kg) or saline (control group) during stabilization. Then, the rats were exposed to 30 min of hypovolemic shock and 30 min of reperfusion. Administration of Lip-ATP significantly improved hepatic blood flow during shock (13.2 +/- 1.7 ml/min/100 g tissue in the Lip-ATP group vs. 9.4 +/- 2.2, in the control group, and 8.9 +/- 2.9 in the free ATP group) and during reperfusion (20.9 +/- 2.3, 16.3 +/- 2.2, and 16.2 +/- 1.8 ml/min/100 g tissue, respectively). The serum levels of hepatic enzymes (ALT, AST and LDH) were significantly lower in the Lip-ATP group after reperfusion when compared with the control and free ATP groups. Administration of Lip-ATP also produced the best recovery of hepatic ATP level. These findings indicate that Lip-ATP increased hepatic blood flow during shock and reperfusion, presumably by improving the energy charge and metabolism of the hepatocytes. Thus, Lip-ATP appears to be a useful agent for liver injury induced by hypovolemic shock.
Konno
H
H
Second Department of Surgery, Hamamatsu University School of Medicine, Japan.
Matin
A F
AF
Maruo
Y
Y
Nakamura
S
S
Baba
S
S
D000255:Adenosine Triphosphate / Q000008:administration & dosage*; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001783:Blood Flow Velocity; D007262:Infusions, Intravenous; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D012769:Shock / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D008081:Liposomes; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase