Relaxation response to the rat stomach fundus to methylene blue.
When methylene blue (10(-4) M) was applied to the rat stomach fundus precontracted with 10(-6) M serotonin in the presence of 10(-6)M atropine and 10(-6) M guanethidine, slowly developing relaxation was induced. Methylene blue (10(-4) M) increased cyclic AMP but not cyclic GMP levels in the stomach fundus. These results suggest that methylene blue may relax the rat stomach fundus through cyclic AMP formation.
Kamata
K
K
Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Tokyo, Japan.
Kohzuki
M
M
Kasuya
Y
Y
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004558:Electric Stimulation; D005748:Gastric Fundus / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006145:Guanethidine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects*; D013565:Sympatholytics / Q000494:pharmacology
D018727:Muscarinic Antagonists; D013565:Sympatholytics; D012701:Serotonin; D001285:Atropine; D000242:Cyclic AMP; D006152:Cyclic GMP; D008751:Methylene Blue; D006145:Guanethidine