Comparison of polymerase chain reaction and monoclonal antibodies for G-typing of group A bovine rotavirus directly from fecal material.
A reverse transcriptase and polymerase chain reaction (RT-PCR)-based assay for G-typing of bovine rotaviruses (BRV) was compared with a monoclonal antibody-based immunoassay (MAbs-ELISA) in the characterization of BRV field strains obtained from calves in different regions of Quebec between 1992 and 1994. The strains were analysed for two G types (G6 and G10) which are the most predominate BRV field strains. Fecal samples positive for BRV by polyacrylamide gel electrophoresis (n = 74) were typed by both methods revealing 77% correlation. RT-PCR detected 10 more G6 and 2 more G10 serotypes than MAbs-ELISA. Nine of the 12 discrepant samples could be cultivated and were confirmed as G6 (8) or G10 (1) by both methods. RT-PCR was able to efficiently detect artificial mixes of G6 and G10 and detected two mixed field infections. Four additional infections considered as mixed by MAbs-ELISA and as only G6 by RT-PCR were possibly MAbs-ELISA cross-reactions. RT-PCR provided a very sensitive method for typing BRV field isolates.
Hussein
H A
HA
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
Frost
E
E
Talbot
B
B
Shalaby
M
M
Cornaglia
E
E
el-Azhary
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002213:Capsid / Q000145:classification*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D012703:Serotyping / Q000379:methods / Q000662:veterinary*
D000911:Antibodies, Monoclonal