Inhibition of glycolysis in boar spermatozoa by alpha-chlorohydrin phosphate appears to be mediated by phosphatase activity.
(R,S)-alpha-chlorohydrin-1-phosphate, previously shown to have no anti-glycolytic activity on mature boar sperm in vitro, is a substrate for acid and/or neutral phosphatase(s) that are associated with washed sperm. The high phosphatase activity hydrolyses the ester to alpha-chlorohydrin which undergoes oxidation to (S)-3-chlorolactaldehyde, a specific inhibitor of sperm glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, thereby exhibiting an anti-glycolytic action.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Porter
L M
LM
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D006019:Glycolysis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D010084:Oxidation-Reduction; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
D004791:Enzyme Inhibitors; C064190:alpha-chlorohydrin-1-phosphate; D005632:Fructose; D000517:alpha-Chlorohydrin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010744:Phosphoric Monoester Hydrolases; D014305:Triose-Phosphate Isomerase