Repetitive hypoxic exposure of brain slices and electrophysiological responses as an experimental model for investigation of cerebroprotective measurements.
An in vitro hippocampal (CA 1 region, guinea pig) slice technique using repeated hypoxia was employed to model electrophysiological changes (DC-potentials and evoked potentials (EP) by stimulation of Schaffer-collaterals) occurring in the hypoxic CA1 pyramidal layer. A standardized neuronal response under repeated hypoxic conditions was observed in this model, consisting of disappearance of EP and a trend towards partially reversible, but progressive synaptic failure subsequent anoxic depolarisation (AD). Slices treated with the calcium antagonist nimodipine showed a prolongation of AD latency between the first and following hypoxias. So it seems possible to simulate hypoxic lesions of the brain tissue by using this in vitro slice model.
Wassmann
H
H
Klinik and Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Germany.
Moskopp
D
D
Woesler
B
B
Lücke
A
A
Speckmann
E J
EJ
Köhling
R
R
Straub
H
H
Hülsmann
S
S
Greiner
C
C
D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000503:physiopathology; D002121:Calcium Channel Blockers / Q000494:pharmacology / Q000627:therapeutic use; D015687:Cell Hypoxia; D004558:Electric Stimulation; D004734:Energy Metabolism; D005071:Evoked Potentials / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology*; D002534:Hypoxia, Brain / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology*; D008564:Membrane Potentials / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D009553:Nimodipine / Q000494:pharmacology / Q000627:therapeutic use; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission
D002121:Calcium Channel Blockers; D018696:Neuroprotective Agents; D009553:Nimodipine