title: Beta 4 integrin and other Schwann cell markers in axonal neuropathy.
abstract: Schwann cell gene expression is dynamically regulated after peripheral nerve injury and during regeneration. We hypothesized that the changes in protein expression described after rat peripheral nerve injury could be used to identify single Schwann cell-axon units in human axonal neuropathy. Therefore, we performed immuno-fluorescence staining on sections of injured rat sciatic nerves compared with sections of neuropathic human sural nerves. We chose the markers beta 4 integrin, P0 glycoprotein, and glial fibrillary acidic protein (GFAP) to characterize Schwann cells, and neurofilament-heavy (NF-H) to recognize axons. Normal rat or human myelin-forming units demonstrated a sharp ring of beta 4 staining at their outer surface, P0 staining in the myelin sheath, and NF-H staining in the axon. Acutely denervated rat units transited from broken rings of beta 4 and P0 staining, to diffuse beta 4 and absent P0 and NF-H staining. Chronically denervated rat Schwann cells re-expressed beta 4 more highly, but in a diffuse, non-polarized pattern. In contrast, regenerating units re-expressed beta 4, P0, and NF-H; beta 4 staining was polarized to the outer surface of Schwann cells. Finally, GFAP staining increased progressively after injury and decreased during regeneration in the distal nerve stump. In neuropathic human sural nerves, we identified units exhibiting each of these beta 4, P0, and NF-H staining patterns; the proportion of each pattern correlated best with the extent and chronicity of axonal injury. Thus, synchronous injury of rat sciatic nerve predicts patterns of Schwann cell marker expression in human axonal neuropathy. In addition, the unique changes in the polarity of beta 4 integrin expression, in combination with changes in P0 and NF-H expression, may distinguish normal from denervated or reinnervated myelin-forming Schwann cells in human sural nerve biopsies.
authors.affiliation: Department of Neurology, San Raffaele Scientific Institute, Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Quattrini
authors.forename: S
authors.initials: S
authors.lastname: Previtali
authors.forename: M L
authors.initials: ML
authors.lastname: Feltri
authors.forename: N
authors.initials: N
authors.lastname: Canal
authors.forename: R
authors.initials: R
authors.lastname: Nemni
authors.forename: L
authors.initials: L
authors.lastname: Wrabetz
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001369:Axons / Q000378:metabolism / Q000502:physiology; D015415:Biomarkers; D019084:Fluorescent Antibody Technique, Indirect; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D039663:Integrin beta4; D008875:Middle Aged; D018993:Myelin P0 Protein / Q000378:metabolism; D009409:Nerve Crush; D016900:Neurofilament Proteins / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries; D013497:Sural Nerve / Q000378:metabolism / Q000473:pathology; D014949:Wounds, Nonpenetrating / Q000378:metabolism / Q000473:pathology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D039663:Integrin beta4; D018993:Myelin P0 Protein; D016900:Neurofilament Proteins; C051774:neurofilament protein H