title: The function of type IV collagen during Drosophila muscle development.
abstract: Type IV collagen forms a network that provides the major structural support for basement membranes. Basement membranes are specialized forms of extracellular matrix with important functions in development. One collagen gene (Dcg1) was characterized in Drosophila melanogaster and shown to encode a collagen chain related to vertebrate basement membrane type IV collagen chains. Therefore, to access the functional importance of type IV collagen during Drosophila myogenesis, we adopted two different approaches to decrease the Dcg1 gene expression in Drosophila embryos. We describe, here, that the decrease in Dcg1 gene expression causes, in particular, defective muscle attachments. These mutant phenotypes suggest that type IV collagen acts to stabilize cell-matrix interactions.
authors.affiliation: Institut de Biologie du Développement de Marseille, CNRS-INSERM-Université Aix-Marseille II, France.
authors.forename: C
authors.initials: C
authors.lastname: Borchiellini
authors.forename: J
authors.initials: J
authors.lastname: Coulon
authors.forename: Y
authors.initials: Y
authors.lastname: Le Parco
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003094:Collagen / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000737:chemistry; D017344:Genes, Insect; D005804:Genes, Lethal; D005821:Genetic Techniques; D007814:Larva / Q000737:chemistry / Q000254:growth & development; D008854:Microscopy, Electron; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000196:embryology* / Q000254:growth & development; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D003094:Collagen