Characterisation of leucocytic somatic cells in bovine milk.
The mammary gland is of cutaneous origin with stromal leucocytic infiltrates and it excretes leucocytes in the milk. Milk T lymphocytes are highly enriched by sedimenting milk somatic cells in a 43 per cent preformed Percoll gradient. Thirty to 54 per cent of leucocytes, harvested from the upper Percoll band, belong to CD2+ CD3+ T lymphocytes. These milk T lymphocytes are mature CD4+, or CD8+ single positive T cells, expressing CD44, CD26, and beta 1 integrin, but not Lam-1. Milk T lymphocyte subsets may play an important role in regional immunosurveillance of the mammary epithelia and stroma. Selective mechanisms may account for the trafficking of this activated, memory T lymphocyte subset in the gland.
Schmaltz
R
R
Department of Veterinary Biomedical Sciences, IANR, University of Nebraska-Lincoln 68583-0905, USA.
Bhogal
B
B
Wang
J
J
Wang
Y Y
YY
Mackay
C R
CR
Chen
S S
SS
Larson
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D018801:CD2 Antigens / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002417:Cattle; D002469:Cell Separation; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000032:analysis; D016130:Immunophenotyping; D019012:Integrin beta1 / Q000032:analysis; D007962:Leukocytes / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000276:immunology; D008892:Milk / Q000166:cytology* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D018819:Dipeptidyl Peptidase 4