Different PrlA proteins increase the efficiency of periplasmic production of human interleukin-6 in Escherichia coli.
The export efficiency of a fusion of the Escherichia coli preOmpA signal peptide to human interleukin-6 can be significantly raised by coexpressing three different prlA alleles of sec Y along with wild type secE. The effect seems prlA-specific, as prlG1 (a prl allele of secE) does not affect the export of preOmpA-hIL-6. Coexpression of secD and secF also stimulates the export of the fusion protein.
Pérez-Pérez
J
J
Departamento de Investigación, Pharmacia and Upjohn, Antonio López, Madrid, Spain.
Barbero
J L
JL
Márquez
G
G
Gutiérrez
J
J
D000483:Alleles; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D000069816:SEC Translocation Channels
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015850:Interleukin-6; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D000069816:SEC Translocation Channels; C499394:SecD protein, E coli; C065493:SecE protein, E coli; C489583:SecF protein, E coli; C057841:SecY protein, E coli; C075767:secD protein, Bacteria; C075768:secF protein, Bacteria