title: The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae.
abstract: To better understand the process of protein sorting to the yeast vacuole, the VPS8 gene was identified and characterized. VPS8 encodes a membrane-associated hydrophilic protein of 135 kDa (Vps8p), which is required for the accurate sorting of the vacuolar hydrolase, carboxypeptidase Y (CPY). vps8 mutant cells missort and secrete CPY as well as a second soluble vacuolar hydrolase, proteinase A. In vps8 mutants, several late-Golgi membrane proteins fail to be retained in the Golgi apparatus. The Golgi-localized CPY sorting receptor, Vps10p, is mislocalized to and aberrantly proteolyzed in the vacuole. Based on our findings, we propose that Vps8p is part of a protein complex that associates with Golgi and post-Golgi membranes and functions in the retrieval of Golgi membrane proteins from the prevacuolar compartment.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chen
authors.forename: T H
authors.initials: TH
authors.lastname: Stevens
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000502:physiology*; D003001:Cloning, Molecular; D019084:Fluorescent Antibody Technique, Indirect; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000502:physiology*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014617:Vacuoles / Q000235:genetics*; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; C087335:PEP1 protein, S cerevisiae; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C103153:VPS8 protein, S cerevisiae; D033921:Vesicular Transport Proteins