title: Stimulation of microtubule dynamic turnover in living cells treated with okadaic acid.
abstract: We have examined the effects of okadaic acid, an inhibitor of protein phosphatases type 1 and 2A, on the dynamic instability behavior of individual microtubules in living cells. Addition of 1 microM okadaic acid to PtK1 epithelial cells induced ruffling of lamellar regions; after 50 min in okadaic acid, many cells were observed to round up. Confocal microscopy of okadaic acid-treated cells stained with an antibody to tubulin showed that microtubules were more densely packed near the periphery of the rounded cells, and in many cells, a reduction in the density of microtubules near the microtubule-organizing center was observed. The dynamic behavior of individual microtubules in cells previously injected with rhodamine-labeled tubulin was quantified by tracking individual microtubules from image sequences. Microtubule dynamic turnover was markedly stimulated in cells treated with 1 microM okadaic acid for 50-60 min: The average rates of both microtubule growing and shortening increased, and the average duration of pause, or attenuation, a phase in which neither growth nor shortening could be detected, was significantly decreased. Further, okadaic acid induced an approximately twofold increase in the frequency of catastrophe transitions and a threefold decrease in the frequency of rescue transitions. Dynamicity, a measure of the net gain and loss of polymer at microtubule plus ends, increased nearly threefold in okadaic acid-treated cells. These results demonstrate that microtubule turnover is stimulated in okadaic acid-treated cells and suggest that phosphorylation of molecules which interact with microtubules may result in increased microtubule dynamic turnover in vivo.
authors.affiliation: Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, USA.
authors.forename: E
authors.initials: E
authors.lastname: Shelden
authors.forename: P
authors.initials: P
authors.lastname: Wadsworth
mesh_terms: D000818:Animals; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium; D007614:Macropodidae; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D019319:Okadaic Acid / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D014404:Tubulin / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; D014404:Tubulin; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases