Endogenous gamma interferon and interleukin-10 in Brucella abortus 2308 infection in mice.
CD-1 mice intravenously infected with the virulent Brucella abortus 2308 strain simultaneously produce significant levels of gamma interferon (IFN-gamma) and interleukin-10 (IL-10) in their spleens between the second and eighth day post-infection with no production of interleukin-4 (IL-4). Endogenous synthesis of IL-10 does not affect the production of IFN-gamma in this organ, while the production of both cytokines during this period of time is accompanied by a statistically significant increase (P < 0.001) in the number of colony forming units (cfu) of B. abortus 2308 present in the organ. These findings suggest that although the endogenous synthesis of IL-10 apparently does not affect IFN-gamma production, it may affect the effector functions of macrophages to control intracellular brucellae. Production of the Th1 cytokine IFN-gamma during B. abortus 2308 infection is also associated with a specific IgG3 and IgG2a response against the B. abortus 2308 lipopolysaccharide (S-LPS) antigen.
Fernández-Lago
L
L
Departamento de Microbiología y Genética, Facultad de Farmacia, Universidad de Salamanca, Spain.
Monte
M
M
Chordi
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D002003:Brucella abortus / Q000254:growth & development / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000382:microbiology; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma