Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in women with or without gynecologic cancer.
OBJECTIVE
To detect the level of 8-hydroxy-2'-deoxyguanosine (8-OHdG) which is an oxygen-radical-forming agent, in the urine of patients with (n = 18) or without (n = 10) carcinoma of the female genitalia. None of the patients had been receiving any treatment before their urinary 8-OHdG levels were measured.


METHODS
Urinary 8-OHdG was extracted by a solid-phase technique, and its level was determined by high-performance liquid chromatography (HPLC) with an electric chemical detector (ECD).


RESULTS
We determined that the urinary 8-OHdG level decreased with as the age of the patient increased, and was extremely high in advanced cancer and recurrent cancer in a considerable number of patients. The urinary 8-OHdG level (1,827 +/- 1,500 pmol/kg/day, mean +/- SEM) in 18 patients with carcinoma was significantly higher (p < or = 0.05) than that (747 +/- 425 pmol/kg/day) in 10 patients without carcinoma.


CONCLUSIONS
These results suggest that it might be possible to determine the spread of cancer to some extent by determining a patient's urinary 8-OHdG level.
Yamamoto
T
T
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
Hosokawa
K
K
Tamura
T
T
Kanno
H
H
Urabe
M
M
Honjo
H
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000375:Aging / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D005833:Genital Neoplasms, Female / Q000652:urine*; D006801:Humans; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000652:urine; D010051:Ovarian Neoplasms / Q000652:urine; D002583:Uterine Cervical Neoplasms / Q000652:urine; D014625:Vaginal Neoplasms / Q000652:urine; D014846:Vulvar Neoplasms / Q000652:urine
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine