title: Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells.
abstract: Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; there-after, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.
authors.affiliation: Department of Internal Medicine, University of Rome Tor Vergata, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Borboni
authors.forename: R
authors.initials: R
authors.lastname: Magnaterra
authors.forename: R A
authors.initials: RA
authors.lastname: Rabini
authors.forename: R
authors.initials: R
authors.lastname: Staffolani
authors.forename: O
authors.initials: O
authors.lastname: Porzio
authors.forename: G
authors.initials: G
authors.lastname: Sesti
authors.forename: A
authors.initials: A
authors.lastname: Fusco
authors.forename: L
authors.initials: L
authors.lastname: Mazzanti
authors.forename: R
authors.initials: R
authors.lastname: Lauro
authors.forename: L N
authors.initials: LN
authors.lastname: Marlier
mesh_terms: D000818:Animals; D001483:Base Sequence; D001710:Biotin / Q000494:pharmacology*; D002478:Cells, Cultured; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005941:Glucokinase / Q000235:genetics* / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013997:Time Factors
chemical_list: D017931:DNA Primers; D007328:Insulin; D012333:RNA, Messenger; D001710:Biotin; D005941:Glucokinase