Regulation of acetyl coenzyme-A carboxylase gene in a transgenic animal model.
The gene for acetyl-CoA carboxylase, the rate-limiting enzyme in the biosynthesis of long-chain fatty acids, contains two promoters; promoter I (PI) and promoter II (PII) which are responsible for generation of class I and class II ACC mRNAs, respectively. Class I ACC mRNAs are present in adipose tissue, but only a trace was found in the liver under normal physiological conditions. However, class I mRNAs were induced under stimulated lipogenic conditions. To investigate how PI is regulated in vivo, we generated transgenic mice containing a reporter gene under the control of PI. In transgenic mice, PI is generally inactive and a small amount of PI activity was found only in the adipose tissues of female animals. Stimulated lipogenic conditions activated PI about 17-fold over normal conditions and again only in white adipose tissues of female animals.
Ekhterae
D
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Biochemistry Department, Purdue University, West Lafayette, Indiana 47907, USA.
Tae
H J
HJ
Daniel
S
S
Moller
D E
DE
Kim
K H
KH
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis* / Q000235:genetics*; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011555:Pseudopregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase; D000103:Acetyl-CoA Carboxylase