title: Influence of 17- alpha-estradiol on catecholamine secretion from the perfused rat adrenal gland.
abstract: OBJECTIVE
  It has been known that adrenal corticosteroids influence the expression of adrenomedullary catecholamine-synthetizing enzymes and also suppress the emission of axonal-like processes in cultured chromaffin cells. In the present study, it was attempted to investigate the effect of 17-alpha-estradiol on catecholamine (CA) secretion evoked by acetylcholine (ACh). DMPP. McN-A-343, excess K+ and Bay-K-8644 from the isolated perfused rat adrenal gland.
  
  
  METHODS
  Mature male Sprague-Dawley rats were anesthetized with ether. The adrenal gland was isolated by the method of WaKade. A cannula used for perfusion of the adrenal gland was inserted into the distal end of the renal vein. The adrenal gland, along with ligated blood vessels and the cannula, was carefully removed from the animal and placed on a platform of a leucite chamber.
  
  
  RESULTS
  The perfusion of 17-alpha-estradiol (1-100 uM) into an adrenal vein for 20 min produced relatively dose-dependent inhibition in CA secretion evoked by ACh (5.32 mM). DMPP (100 uM for 2 min). McN-A-343 (100 uM for 2 min) and Bay-K-8644 (10 uM for 4 min), while it did not affect the CA secretory effect of high K+ (56 mM). Also, in the presence of 17-beta-estradiol. CA secretion of ACh. DMPP and McN-A-343, without any effect on excess K(+)-evoked CA sectretion was depressed. However, in adrenal glands pre-loaded with 17-alpha-estradiol (10 uM) plus tamoxifen (2 uM), which is known to be a selective antagonist of estrogen receptors (for 20 min). CA secretory responses evoked by ACh. DMPP and McN-A-343 were condiderably recovered as compared to that of 17-alpha-estradiol only, but excess K(+)-induced CA secretion was not affected. However, pre-treatment with 17-alpha-estradiol in the presence of meclopramide (dopaminergic antagonist) did not affect the secretory effect of CA evoked by ACh. DMPP, McN-A-343 and high potassium.
  
  
  CONCLUSIONS
  These results suggest that 17-alpha-estradiol causes the marked inhibition of CA secretion evoked by cholinergic receptor stimulation, but not that by excess K+, indicating strongly that this effect may be mediated by inhibiting the influx of extracellular calcium into the rat adrenomedullary chromaffin cells through the activation of inhibitory estrogen receptors, and it also plays a modulatory role in regulating CA secretion.
authors.affiliation: Department of Internal Medicine and Pharmacology, Chosun University, Kwang Ju, Korea.
authors.forename: Y H
authors.initials: YH
authors.lastname: Park
authors.forename: G S
authors.initials: GS
authors.lastname: Cho
authors.forename: E T
authors.initials: ET
authors.lastname: Cho
authors.forename: Y K
authors.initials: YK
authors.lastname: Park
authors.forename: M J
authors.initials: MJ
authors.lastname: Lee
authors.forename: J Y
authors.initials: JY
authors.lastname: Chung
authors.forename: S P
authors.initials: SP
authors.lastname: Hong
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Jang
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoo
authors.forename: C H
authors.initials: CH
authors.lastname: Choi
authors.forename: D Y
authors.initials: DY
authors.lastname: Lim
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D046508:Culture Techniques; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D010477:Perfusion / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002395:Catecholamines; D004958:Estradiol