title: Properties of 26S proteasome purified from rat skeletal muscles: comparison with those of 26S proteasome from the rat brain.
abstract: A ubiquitin/ATP-dependent proteolytic complex (26S proteasome) was highly purified from rat skeletal muscles and its enzymatic properties were compared with those of the brain 26S proteasome. The purified 26S proteasome comprises 22-110 kDa subunits characteristic of the typical 26S proteasome on the basis of SDS-PAGE. The two-dimensional PAGE (NEPHGE and SDS-PAGE) pattern revealed that the pI values and molecular masses of the muscle 26S proteasome subunits were similar but not identical to those of the subunits of 26S proteasome purified from the rat brain. The enzymatic properties of the muscle 26S proteasome were very similar to those of the brain enzyme in substrate specificity and inhibitor susceptibility. The specific activities of the muscle 26S proteasome toward three fluorogenic peptide substrates were indistinguishable from those of the brain enzyme.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akaishi
authors.forename: H
authors.initials: H
authors.lastname: Sawada
authors.forename: H
authors.initials: H
authors.lastname: Yokosawa
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease