Viral isolates derived from simian varicella epizootics are genetically related but are distinct from other primate herpesviruses.
Epizootics of a natural varicella-like disease occur in populations of nonhuman primates. Several primate herpesviruses have been isolated from these epizootics, but the relatedness of these isolates to each other is not well-defined. In this study, we demonstrated that the restriction endonuclease (REn) profiles of four epidemiologically distinct isolates were similar, although not identical, indicating that simian varicella epizootics are caused by various strains of simian varicella virus (SVV). The genetic variation among the isolates did not map to a specific region of the SVV genome and REn differences were detected within the SVV DNA long component and the inverted repeat region. Southern blot hybridization demonstrated that SVV is more closely related to varicella-zoster virus than to other primate herpesviruses. The study indicates that the current herpesvirus classification scheme should be changed to include SVV as a single taxonomic group within the Varicellovirus genus of alphaherpesviruses. In addition, REn profiles of SVV isolates, derived from primary and secondary episodes of simian varicella in the same monkey, were identical, providing evidence for SVV reactivation in a latently infected monkey.
Gray
W L
WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA. wgray@biomed.uams.edu
Gusick
N J
NJ
D000818:Animals; D001426:Bacterial Proteins; D002522:Chlorocebus aethiops; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006564:Herpesviridae / Q000145:classification; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D018419:Primate Diseases / Q000821:virology; D015183:Restriction Mapping; D014709:Vero Cells
D001426:Bacterial Proteins; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific