title: Partial purification and characterization of Salmonella cytotoxin.
abstract: The cytotoxins of two strains of Salmonella enterica subspecies enterica serovar Nchanga (MP2309) and Virchow (MP1871) were partially purified and characterized physicochemically. The toxin was salt-precipitable and non-dialysable. Upon filtration through Sephadex G-100 gel column, the toxicity was observed mainly in 2nd peak. The cytotoxic activity was lost completely when heated at 121 degrees C for 15 min. The toxin was found active between a narrow range of pH (i.e., 6.0 to 9.0). The cytotoxic activity was destroyed by treatment with trypsin and protease but the activity was not affected by papain.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, India.
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: V D
authors.initials: VD
authors.lastname: Sharma
authors.forename: D C
authors.initials: DC
authors.lastname: Thapliyal
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003470:Culture Media; D003603:Cytotoxins / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D006358:Hot Temperature; D012475:Salmonella / Q000737:chemistry / Q000254:growth & development; D014709:Vero Cells
chemical_list: D003470:Culture Media; D003603:Cytotoxins; D004768:Enterotoxins