title: Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study.
abstract: Spectroscopic study on the interactions of trace elements Co., Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These studies also showed lower concentrations of Mn displaced EtBr, whereas Al could displace it at higher ionic strength. Mg and Co displaced EtBr fluorescence at moderate concentrations. The binding constant values and CD spectra clearly indicated strong binding of these elements to DNA.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
authors.forename: M T
authors.initials: MT
authors.lastname: Rajan
authors.forename: D C
authors.initials: DC
authors.lastname: Kumar
authors.forename: D
authors.initials: D
authors.lastname: Vishnuvardhan
authors.forename: K S
authors.initials: KS
authors.lastname: Rao
authors.forename: M A
authors.initials: MA
authors.lastname: Viswamitra
mesh_terms: D000535:Aluminum / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D003035:Cobalt / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D008670:Metals; D009838:Oligodeoxyribonucleotides; D003035:Cobalt; D008345:Manganese; D004247:DNA; D000535:Aluminum; D008274:Magnesium