Expression of the 14 kDa galactose-binding protein, galectin-1, on human tubular epithelial cells.
By reverse phase PCR and Northern blotting, RNA of the 14 kDa galactose-binding protein (galectin-1) could be identified in primary cultures of human tubular epithelial cells. To assess protein synthesis and the possible function of galectin-1 on TEC, the cellular proteins were biosyntheticically labeled with [34S]-methionine and absorbed to immobilized laminin. Multiple radiolabeled proteins were eluted, a strong band in the area of 14 kDa was seen, coinciding with the galectin-1 band as identified by Western blotting. Surface expression of galectin-1 was seen by cytofluorometry with two different polyclonal antibodies to galectin-1. These data are in line with the finding that tubular epithelial cells adhere to laminin, partly in a Ca(2+)-independent manner.
Bürger
A
A
Institut für Immunologie, Universität Heidelberg, Germany.
Filsinger
S
S
Cooper
D N
DN
Hänsch
G M
GM
D000954:Antigens, Surface / Q000096:biosynthesis; D015152:Blotting, Northern; D015153:Blotting, Western; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D005690:Galactose / Q000378:metabolism*; D037483:Galectin 1; D015870:Gene Expression / Q000502:physiology; D018922:HL-60 Cells / Q000737:chemistry / Q000502:physiology; D006388:Hemagglutinins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011485:Protein Binding / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism
D000954:Antigens, Surface; D037483:Galectin 1; D006388:Hemagglutinins; D007797:Laminin; D012333:RNA, Messenger; D002118:Calcium; D005690:Galactose