Factors of Ehrlich Ascites Tumor resistance to oxidative stress.
Ehrlich Ascites Tumor (EAT) cells, compared to liver, were less susceptible to peroxidation (measured by the TBARS method) induced by Fe2+/ascorbate, ADP/Fe2+ and H2O2/Fe2+/ascorbate. Higher thiobarbituric acid reagent products TBARS levels were found with Fe2+/ascorbate or after ultrasonication induced peroxidation in lipids obtained from Ehrlich cells compared to EAT cell homogenate with the same lipid content. This suggests that non-lipid and structurally membrane antioxidants factors are of less importance for induction by Fe2+/ascorbate than by ultrasonication. The resistance to induced lipid peroxidation (LPO) in EAT cells is associated with the lower level of lipids, the lower double-bond index, the lower level of fatty acids reacting with TBA, as well as with the relatively higher level of alpha-tocopherol per mg lipid in the EAT cells as compared to the liver.
Hristov
P
P
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Tyurin
V
V
Petrov
L
L
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000502:physiology; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014465:Ultrasonics; D014810:Vitamin E / Q000378:metabolism
D005227:Fatty Acids; D005290:Ferric Compounds; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D000244:Adenosine Diphosphate; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid