title: Development of a diagnostic DNA probe to detect Setaria digitata: the causative parasite of cerebrospinal nematodiasis in goats, sheep and horses.
abstract: Two repetitive sequences (IpSdM and IpSdS) have been cloned and sequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the S. digitata genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both S. digitata and S. labiato-papillosa. It can also detect a single microfilaria and a L3 larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka.
authors.forename: W S
authors.initials: WS
authors.lastname: Wijesundera
authors.forename: N V
authors.initials: NV
authors.lastname: Chandrasekharan
authors.forename: E H
authors.initials: EH
authors.lastname: Karunanayake
authors.forename: S P
authors.initials: SP
authors.lastname: Dharmasena
mesh_terms: D000818:Animals; D015342:DNA Probes; D015511:Goat Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*; D006041:Goats; D006734:Horse Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*; D006736:Horses; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D017182:Setaria Nematode / Q000235:genetics*; D012719:Setariasis / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D012756:Sheep; D012757:Sheep Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*
chemical_list: D015342:DNA Probes