Thin-layer chromatographic determination of catecholamines in rat plasma.
A TLC method for the quantitative determination of epinephrine, norepinephrine and dopamine in rat plasma is described. After deprotenization, catecholamines were adsorbed on acid-alumina and acetylated. The acetyl derivatives were extracted using a C18 minicolumn, resolved on HPTLC plates and quantitated by fluorescence densitometry at 415 nm, using isoprenaline as internal standard.
Alemany
G
G
Dept. de Biología Fonamental i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Akaârir
M
M
Rosselló
C
C
Gamundi
A
A
D000818:Animals; D002395:Catecholamines / Q000097:blood*; D002855:Chromatography, Thin Layer / Q000379:methods*; D002940:Circadian Rhythm; D003720:Densitometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D002395:Catecholamines