Tracking of dye-labeled lymphocytes in rhesus macaques.
Lymphocytes were isolated from rhesus monkeys and marked with a fluorescent lipophilic dye to monitor their distribution in vivo. Dye-labeled cells were either monitored by blood draws over a three-month period, or identified within peripheral organs upon autopsy. Lymphocyte labeling conditions were optimized. Dye-labeled lymphocytes could be detected in the circulation for at least 100 days by flow cytometry and fluorescence microscopy. Activated lymphocytes were removed from the circulation more rapidly than lymphocytes that had not been activated.
Salvato
M S
MS
Dept. of Pathology and Laboratory Medicine, University of Wisconsin, Madison, USA.
Rater
M
M
Pauza
C D
CD
D000818:Animals; D018951:Antigens, CD20 / Q000032:analysis; D001344:Autopsy; D018801:CD2 Antigens / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D016130:Immunophenotyping; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008253:Macaca mulatta; D008297:Male; D008856:Microscopy, Fluorescence; D015375:Receptors, Interleukin-2 / Q000032:analysis
D018951:Antigens, CD20; D018801:CD2 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D005456:Fluorescent Dyes; D018950:Lipopolysaccharide Receptors; D015375:Receptors, Interleukin-2