Growth inhibition of K-ras-expressing tumours by a new vinca alkaloid, conophylline, in nude mice.
Conophylline, a new vinca alkaloid isolated from the plant Ervatamia microphylla induced normal flat morphology in K-ras-NRK and K-ras-NIH cell lines, and lowered the increased uptake of 2-deoxyglucose in K-ras-NRK cells. Conophylline inhibited the growth of K-ras-NRK cells, but this inhibition was reversible. The alkaloid also inhibited the growth of K-ras-NRK and K-ras-NIH3T3 tumours transplanted into nude mice. On the other hand, it showed no effect on survival of the mice loaded with L1210 leukaemia. Thus, conophylline is a new antitumour vinca alkaloid that induced normal phenotypes in ras-expressing cells.
Umezawa
K
K
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Taniguchi
T
T
Toi
M
M
Ohse
T
T
Tsutsumi
N
N
Yamamoto
T
T
Koyano
T
T
Ishizuka
M
M
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002417:Cattle; D004354:Drug Screening Assays, Antitumor; D005260:Female; D011905:Genes, ras / Q000187:drug effects*; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014748:Vinca Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D014748:Vinca Alkaloids; C099167:conophylline