title: Biofilms associated with poultry processing equipment.
abstract: Aerobic and Gram-negative bacteria were enumerated on non-metallic surfaces and stainless steel test pieces attached to equipment surfaces by swabbing and a mechanical dislodging procedure, respectively, in a South African grade B poultry processing plant. Changes in bacterial numbers were also monitored over time on metal test pieces. The highest bacterial counts were obtained from non-metallic surfaces such as rubber fingered pluckers and plastic defeathering curtains which exceeded the highest counts found on the metal surfaces by at least 1 log CFU cm-2. Gram-negative bacterial counts on all non-metallic surface types were at least 2 log CFU cm-2 lower than corresponding aerobic plate counts. On metal surfaces, the highest microbial numbers were obtained after 14 days exposure, with aerobic plate counts ranging from 3.57 log CFU cm-2 to 5.13 log CFU cm-2, and Gram-negative counts from 0.70 log CFU cm-2 to 3.31 log CFU cm-2. Scanning electron microscopy confirmed the presence of bacterial cells on non-metallic and metallic surfaces associated with poultry processing. Rubber 'fingers', plastic curtains, conveyor belt material and stainless steel test surfaces placed on the scald tank overflow and several chutes revealed extensive and often confluent bacterial biofilms. Extracellular polymeric substances, but few bacterial cells were visible on test pieces placed on evisceration equipment, spinchiller blades and the spinchiller outlet.
authors.affiliation: Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: D
authors.initials: D
authors.lastname: Lindsay
authors.forename: I
authors.initials: I
authors.lastname: Geornaras
authors.forename: A
authors.initials: A
authors.lastname: von Holy
mesh_terms: D000818:Animals; D001420:Bacteria, Aerobic; D018441:Biofilms; D015169:Colony Count, Microbial; D004866:Equipment Contamination; D005511:Food Handling; D006090:Gram-Negative Bacteria; D008855:Microscopy, Electron, Scanning; D011200:Poultry; D013997:Time Factors