title: Immunochemical studies on human, bovine and pig brain regulatory subunits of cAMP-dependent protein kinase type II.
abstract: Regulatory subunits type II (RII) purified from human, pig and bovine brains were compared using II monoclonal antibodies (MoAb) against bovine brain RII. Bovine RII has at least 5 antigenic sites located in the N-terminal 1-110 residues. Immunochemical difference detected between human and animal RII was more pronounced than between pig and bovine RII. Certain MoAb influenced R-cAMP binding and holoenzyme formation. RII of the three species responded to MoAb attachment in a similar fashion. The results suggest that anchoring of neural protein kinase via the N-terminal part of RII may influence the enzyme activity.
authors.affiliation: Research Center of Molecular Diagnostics & Therapy, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Sveshnikova
authors.forename: N A
authors.initials: NA
authors.lastname: Alexandrova
authors.forename: I D
authors.initials: ID
authors.lastname: Grozdova
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D003429:Cross Reactions; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes; D006801:Humans; D015151:Immunoblotting; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D000242:Cyclic AMP; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases