Analysis of MyoD, myogenin, and muscle-specific gene mRNAs in regenerating Xenopus skeletal muscle.
We have analyzed in adult Xenopus laevis, using in situ hybridization, the spatial and temporal expression patterns of MyoD, myogenin, and alpha-skeletal actin and fast myosin heavy chain mRNAs during muscle regeneration following cardiotoxin injury. MyoD transcripts could be detected in the satellite cells as early as the first stage of regeneration and were expressed persistently throughout the regeneration process. Myogenin mRNAs were transiently expressed in forming myotubes. alpha-Skeletal actin and fast myosin heavy chain mRNAs were detected precociously, before the young myotube stage. This work has shown, for the first time, the presence of myogenin transcripts during Xenopus myogenesis.
Nicolas
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Laboratoire de Biologie du Développement, Centre Universitaire des Saints-Pères, Université René Descartes, Paris, France.
Gallien
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CL
Chanoine
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C
D000199:Actins / Q000235:genetics; D000818:Animals; D017403:In Situ Hybridization; D018482:Muscle, Skeletal / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics*; D018008:Myogenin / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics; D015347:RNA Probes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012038:Regeneration / Q000235:genetics*; D014982:Xenopus laevis
D000199:Actins; D017570:MyoD Protein; D018008:Myogenin; D015347:RNA Probes; D012333:RNA, Messenger; D018995:Myosin Heavy Chains