Quantitative urinary excretion of unmetabolised N tau-[Me-14C] methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia.
Six common ringtail possums (Pseudocheirus peregrinus) were intravenously injected with a standard dose of radioactive 3-Methylhistidine (N tau-[Me-14C]MeH). The dose was rapidly and quantitatively excreted by the possums. More than 90% of radioactivity was recovered within 3 days. Thin layer chromatography and mass spectroscopy showed that 97% of recovered radioactivity was associated with unmetabolised N tau-[Me-14C]MeH. These data satisfy two key requirements for the validity of urinary 3-Methylhistidine (N tau-3MeH) excretion as an index of muscle protein catabolism, in P. peregrinus.
Watson
S
S
Department of Zoology, James Cook University, Townsville, QLD Australia.
Foley
W J
WJ
McLean
S
S
Brandon
S
S
Davies
N W
NW
D000818:Animals; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D005260:Female; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D008762:Methylhistidines / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D009124:Muscle Proteins / Q000378:metabolism; D009893:Opossums / Q000378:metabolism*
D008762:Methylhistidines; D009124:Muscle Proteins; C028118:3-methylhistidine