Effect of mobile phase composition on selectivity in preparative hydrophobic interaction chromatographic purification of glucagon.
A weakly hydrophobic acrylester copolymer, Amberlite XAD-7, has been used in the purification process of glucagon production for elimination of protein impurities in the crude glucagon fractions, obtained from the industrial waste from insulin production. Varying the mobile phases composition, it was shown that suitable selectivity for the separation of proteins under non-denaturing conditions can be achieved. The purified glucagon, obtained at the proposed chromatographic conditions has a biological activity of above 1 IU/mg.
Angelova
M
M
Chemical Pharmaceutical Research Institute, NIHFI, Sofia, Bulgaria.
Dimov
N
N
D000180:Acrylic Resins / Q000737:chemistry*; D000837:Anion Exchange Resins / Q000737:chemistry*; D001681:Biological Assay; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005934:Glucagon / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties
D000180:Acrylic Resins; D000837:Anion Exchange Resins; D011137:Polystyrenes; C015661:amberlite XAD 7; D005934:Glucagon