title: Isolation of high-molecular-length DNA from human skin.
abstract: An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
authors.forename: P V
authors.initials: PV
authors.lastname: Bennett
authors.forename: R W
authors.initials: RW
authors.lastname: Gange
authors.forename: H
authors.initials: H
authors.lastname: Hacham
authors.forename: V S
authors.initials: VS
authors.lastname: Hejmadi
authors.forename: M
authors.initials: M
authors.lastname: Moran
authors.forename: S
authors.initials: S
authors.lastname: Ray
authors.forename: B M
authors.initials: BM
authors.lastname: Sutherland
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D019286:Endopeptidase K / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D012685:Sepharose; D012867:Skin / Q000737:chemistry*; D013048:Specimen Handling
chemical_list: D004247:DNA; D012685:Sepharose; D019286:Endopeptidase K