Detection of Legionella pneumophila serogroup 1 antigen in nonconcentrated urine and urine concentrated by selective ultrafiltration.
We evaluated a commercially available radioimmunoassay technique for detecting the soluble antigen of Legionella pneumophila serogroup 1 using concentrated and nonconcentrated urine. Concentration was achieved by selective ultrafiltration. The sensitivity of the technique was 60.9% for nonconcentrated urine and 80.4% for concentrated samples, with 100% specificity in both cases.
Domínguez
J A
JA
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Facultad de Medicina de la Universidad Autónoma de Barcelona, Badalona, Spain.
Manterola
J M
JM
Blavia
R
R
Sopena
N
N
Belda
F J
FJ
Padilla
E
E
Giménez
M
M
Sabrià
M
M
Morera
J
J
Ausina
V
V
D000942:Antigens, Bacterial / Q000652:urine*; D006801:Humans; D016952:Legionella pneumophila / Q000276:immunology* / Q000302:isolation & purification; D014462:Ultrafiltration
D000942:Antigens, Bacterial