title: Clinical relevance of the breakpoint sites within the M-BCR in 50 patients from Argentina with chronic myeloid leukemia.
abstract: Fifty patients from Argentina with chronic myeloid leukemia (CML) were studied in order to characterize the breakpoint site within the major breakpoint cluster region (M-BCR) and its relationship with the duration of the chronic phase (CP). The DNA digestion with the restriction enzymes: Bgl II, BAM HI and Hind III and hybridization with the 1.2Kb Hind III-Bgl II bcr probe showed that 56% of cases had the breakpoint in 5'M-bcr region and the remaining 44% in 3'M-bcr region. The duration of chronic phase from diagnosis to the onset of the blast crisis (BC) was correlated with the location of the breakpoint within the M-bcr and no statistical differences were observed between the 5' and the 3' groups. These data indicate that the breakpoint site within the bcr gene is not a prognostic indicator of the duration of CP of the disease.
authors.affiliation: Departamento de Genetica, Academia Nacional de Medicina de Buenos Aires, Argentina.
authors.forename: I A
authors.initials: IA
authors.lastname: Giere
authors.forename: I B
authors.initials: IB
authors.lastname: Larripa
mesh_terms: D000293:Adolescent; D000328:Adult; D001118:Argentina; D001752:Blast Crisis; D001853:Bone Marrow / Q000473:pathology; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007223:Infant; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome