title: Cytotoxic T-lymphocytes from HIV-infected individuals recognize an activation-dependent, non-polymorphic molecule on uninfected CD4+ lymphocytes.
abstract: OBJECTIVE
  Correlation of lysis of autologous CD4+ target cells by cytotoxic lymphocytes from HIV-seropositive patients to target activation, viral replication, and major histocompatibility complex (MHC) restriction.
  
  
  METHODS
  Twenty-two HIV-infected patients were evaluated for lysis of activated CD4+ cells, concurrent with measurement of proliferation of the target cells, and with viral replication.
  
  
  METHODS
  Titrated standard 51Cr-release assays for specific effector-to-target cell recognition, blocking antibodies and cell depletion for cell characterization, incorporation of [3H]-thymidine for proliferation, and p24 antigen capture assays for viral replication.
  
  
  RESULTS
  HIV-infected patients had cytotoxic lymphocytes capable of recognizing activated CD4+ target cells in a non-MHC-restricted manner. The lysis depended on the degree of target activation, and was independent of viral replication.
  
  
  CONCLUSIONS
  This cytolytic activity is unique to HIV-infected patients, and is suggestive of activation-induced cell death that may contribute to the progressive depletion of CD4+ lymphocytes during HIV pathogenesis.
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Bienzle
authors.forename: F M
authors.initials: FM
authors.lastname: Smaill
authors.forename: K L
authors.initials: KL
authors.lastname: Rosenthal
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D016655:HIV Core Protein p24 / Q000276:immunology; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D009500:Neutralization Tests; D011110:Polymorphism, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014779:Virus Replication
chemical_list: D016655:HIV Core Protein p24; D008934:Mitogens