title: Neutrophil-related cytokines and neutrophil products in bronchoalveolar lavage fluid of a patient with ANCA negative Wegener's granulomatosis.
abstract: We report a case of Wegener's granulomatosis (WG), with neutrophil accumulation in bronchoalveolar lavage fluid (BALF). Peripheral blood neutrophilia was present but the anti-neutrophil cytoplasmic antibody (ANCA) was negative. The serum and BALF levels of neutrophil-related cytokines, including interleukin (IL)-8, granulocyte colony-stimulating factor (G-CSF) and IL-1 beta, were increased, particularly in BALF. Plasma and BALF levels of neutrophil elastase and defensins, which are released by neutrophils and are potentially toxic to cells, were also elevated. Our findings suggest that neutrophils and neutrophil-related cytokines may play an important role in the pathogenesis of anti-neutrophil cytoplasmic antibody negative as well as anti-neutrophil cytoplasmic antibody positive Wegener's granulomatosis.
authors.affiliation: Third Dept of Internal Medicine, Miyazaki Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mukae
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.forename: J
authors.initials: J
authors.lastname: Ashitani
authors.forename: H
authors.initials: H
authors.lastname: Mashimoto
authors.forename: J
authors.initials: J
authors.lastname: Kadota
authors.forename: M
authors.initials: M
authors.lastname: Nakazato
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: S
authors.initials: S
authors.lastname: Matsukura
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic / Q000032:analysis*; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D023082:Defensins; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis / Q000097:blood; D014890:Granulomatosis with Polyangiitis / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000097:blood; D016209:Interleukin-8 / Q000032:analysis / Q000097:blood; D019272:Leukocyte Elastase / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology*
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001798:Blood Proteins; D016207:Cytokines; D023082:Defensins; D007375:Interleukin-1; D016209:Interleukin-8; D016179:Granulocyte Colony-Stimulating Factor; D019272:Leukocyte Elastase