title: Nuclear magnetic resonance studies of sugar metabolism in the human infant lens.
abstract: We investigated sugar metabolism in lenses isolated from infants with stage-5 retinopathy of prematurity (ROP). The lenses were incubated in 13C-labeled glucose (35.5 mM) or galactose (30 mM) for 24 h at 37.5 degrees C. Six to 8 lenses were pooled for each experiment. 13C-nuclear magnetic resonance spectroscopy was then performed on perchloric acid extracts of these lenses. Active aldose metabolism was indicated by the production of lactate. However, neither sorbitol nor dulcitol was detected. On the other hand, an experimental aldose reductase inhibitor, sorbinil, caused a decrease in the hexose monophosphate shunt activity suggesting an active polyol pathway. Both polyols therefore appeared to have been metabolized further. In addition, galactose metabolism was unique in that it not only involved a galactose-->glucose epimerization, but also a direct galactose-->ribose conversion, and a production of galactose-3-phosphate. Although it is possible that the ROP lenses have adapted to oxidative stress during the postnatal high-oxygen incubation for premature infants, we believe the observed sugar metabolism is representative of the developing human lens.
authors.affiliation: Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: H M
authors.initials: HM
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Xiong
authors.forename: S
authors.initials: S
authors.lastname: Hirose
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Hirose
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D002247:Carbon Isotopes; D002675:Child, Preschool; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D007223:Infant; D007231:Infant, Newborn; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010427:Pentose Phosphate Pathway / Q000502:physiology; D011108:Polymers / Q000378:metabolism; D012178:Retinopathy of Prematurity / Q000150:complications / Q000601:surgery; D014821:Vitrectomy
chemical_list: D002247:Carbon Isotopes; D004791:Enzyme Inhibitors; D007093:Imidazoles; D048289:Imidazolidines; D011108:Polymers; C024617:polyol; D000449:Aldehyde Reductase; C026411:sorbinil; D005947:Glucose; D005690:Galactose