Distribution of intercellular adhesion molecule-1 on leukocytes and corneal endothelium after endotoxin stimulation in rats.
After stimulation with Salmonella typhimurium endotoxin, the intercellular adhesion molecule-1 (ICAM-1) was studied on the corneal endothelium and associated leukocytes in rats using immunoscanning electron microscopy. Two hundred micrograms of the endotoxin was injected in Lewis rats. The corneae were excised at 0-h and 16-h-postinjection time (n = 5, respectively). The corneae were prepared in hypothermic University of Wisconsin (UW) solution for immunoscanning electron microscopy. Histotopographical examination visualized ICAM-1 antigen on cytoplasmic processes of the corneal endothelium, arranged along microfolds, especially at the peaks. In the leukocytes, ICAM-1 was located primarily in morphologically non-specialized domains of the cell body surface, and only rarely scattered on the surface of microvillar projections. We concluded that the endotoxin stimulation can increase ICAM-1 in both corneal endothelium and associated leukocytes. Increased ICAM-1 may be an important factor for the leukocytes to form clustering and adhering to the corneal endothelium.
Yamaguchi
K
K
Department of Ophthalmology, Yamagata University School of Medicine, Japan.
Takahashi
Y
Y
Takahashi
S
S
Shoji
T
T
Yuki
Y
Y
Sasaki
K
K
Tonosaki
A
A
D000818:Animals; D004728:Endothelium, Corneal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004731:Endotoxins / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D051381:Rats; D011917:Rats, Inbred Lew; D012486:Salmonella typhimurium
D004731:Endotoxins; D018799:Intercellular Adhesion Molecule-1