title: 5-Fluorouracil metabolite patterns in viable and necrotic tumor areas of murine colon carcinoma determined by 19F NMR spectroscopy.
abstract: High-resolution 19F NMR spectroscopy at 9.4 T was used to study the difference in the metabolite pattern of 5-fluorouracil (5-FU) between viable and necrotic tissues of C38 murine colon tumors grown in C57BI/6 mice. Studies were performed on perchloric acid extracts of these tumor fractions after 5-FU treatment. The 19F nuclear magnetic resonance spectra exhibited resonances representing 5-FU, the catabolites alpha-fluoro-beta-ureidopropionic acid and alpha-fluoro-beta-alanine, as well as several fluoronucleotide anabolites. The absolute concentrations of anabolites and catabolites and the anabolite-to-catabolite ratio were significantly lower in the necrotic fraction than in the viable tumor fraction 50 min after administration of 5-FU, whereas the absolute concentration of 5-FU was the same. Therefore, in 5-FU metabolism studies with NMR spectroscopy, it is important to consider the necrotic contribution to the tumor volume.
authors.affiliation: Department of Medical Oncology, University Hospital, Nijmegen, The Netherlands.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kamm
authors.forename: A
authors.initials: A
authors.lastname: Heerschap
authors.forename: G
authors.initials: G
authors.lastname: Rosenbusch
authors.forename: I M
authors.initials: IM
authors.lastname: Rietjens
authors.forename: J
authors.initials: J
authors.lastname: Vervoort
authors.forename: D J
authors.initials: DJ
authors.lastname: Wagener
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000378:metabolism*; D005260:Female; D005461:Fluorine; D005472:Fluorouracil / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009336:Necrosis
chemical_list: D005461:Fluorine; D005472:Fluorouracil