title: The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.
abstract: The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The results indicate that adenovirus infection induces the disassembly of coiled bodies and that this effect is probably secondary to the block of host protein synthesis induced by the virus. Furthermore, coiled bodies are shown to be very labile structures, with a half-life of approximately 2 h after treatment of HeLa cells with protein synthesis inhibitors. After blocking of protein synthesis, p80 coilin was detected in numerous microfoci that do not concentrate snRNP. These structures may represent precursor forms of the coiled body, which goes through a rapid cycle of assembly/disassembly in the nucleus and requires ongoing protein synthesis to reassemble.
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine, University of Lisbon, Lisboa, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Rebelo
authors.forename: F
authors.initials: F
authors.lastname: Almeida
authors.forename: C
authors.initials: C
authors.lastname: Ramos
authors.forename: K
authors.initials: K
authors.lastname: Bohmann
authors.forename: A I
authors.initials: AI
authors.lastname: Lamond
authors.forename: M
authors.initials: M
authors.lastname: Carmo-Fonseca
mesh_terms: D000258:Adenovirus Infections, Human / Q000378:metabolism / Q000473:pathology*; D000260:Adenoviruses, Human / Q000378:metabolism / Q000472:pathogenicity*; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure* / Q000821:virology; D006207:Half-Life; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D016253:Microscopy, Immunoelectron; D015335:Molecular Probes / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism
chemical_list: D015335:Molecular Probes; D009687:Nuclear Proteins; D011500:Protein Synthesis Inhibitors; D012367:RNA, Viral; D017411:Ribonucleoproteins, Small Nuclear; C068921:p80-coilin