Decreased in vitro chemosensitivity of tumour cells in patients suffering from malignant diseases with poor prognosis.
The aim of the study was to assess the predictive value of MTT in vitro assay for evaluation of tumour cell resistance/sensitivity to cytotoxic drugs. We analyzed 105 samples of malignant cells of different origin. The study included patients with a diagnosis of acute and chronic lymphatic leukaemia, acute and chronic myeloid leukaemia, non-Hodgkin lymphoma, carcinoma of the lung, stomach and liver, rhabdomyosarcoma and breast carcinoma. The results demonstrate outstanding chemosensitivity in the majority of childhood acute lymphoblastic leukaemias, medium chemosensitivity of adult haematopoietic malignant diseases and chemoresistance of solid tumour cells. Our preliminary data suggest a good correlation between in vitro MTT assay and clinical curability of individual malignant diseases.
Hajdúch
M
M
Institute of Immunology, Faculty of Medicine, Palack y University, Olomouc, Czech Republic.
Mihál
V
V
Minarík
J
J
Faber
E
E
Safárovă
M
M
Weigl
E
E
Antálek
P
P
D000328:Adult; D002648:Child; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D019337:Hematologic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000401:mortality; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000401:mortality; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D011237:Predictive Value of Tests; D011379:Prognosis; D016019:Survival Analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects