Cytogenetic effects in mice of divinylbenzene-55 inhalation.
Male B6C3F1 mice (8 weeks of age) were exposed by inhalation to divinylbenzene-55 (DVB-55), at target concentrations of 0, 25, 50 and 75 ppm for 6 h per day for 3 days. Following exposure the animals were killed blood smears were prepared for micronucleus (MN) analysis, and the spleens were removed and cultured for sister chromatid exchange (SCE) and chromosome aberration (CA) analyses. DVB-55 induced a dose dependent increase in SCE with the two highest doses reaching statistical significance. Similarly, there was a statistically significant although less pronounced increase in the frequency of CAs in splenocytes and MN in polychromatic erythrocytes. There was no indication of toxicity as measured by cell cycle kinetics in the splenocytes or the percentage of polychromatic erythrocytes in the peripheral blood smears. Thus, DVB-55 appears to be a weak genotoxicant in vivo.
Kligerman
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Environmental Carcinogenesis Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA. kligerman@her145.Her1.Epa.Gov
Morgan
D L
DL
Doerr
C L
CL
Milholland
V
V
Tennant
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AH
D000280:Administration, Inhalation; D000818:Animals; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D012854:Sister Chromatid Exchange / Q000187:drug effects; D020058:Styrene; D013343:Styrenes / Q000633:toxicity; D014753:Vinyl Compounds / Q000008:administration & dosage / Q000633:toxicity*
D013343:Styrenes; D014753:Vinyl Compounds; D020058:Styrene; C004985:divinyl benzene