A low-CO2-inducible gene encoding an alanine: alpha-ketoglutarate aminotransferase in Chlamydomonas reinhardtii.
At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One full-length clone is 2552 bp, with an open reading frame encoding 521 amino acids. The deduced amino acid sequence shows about 50% identity with alanine: alpha-ketogutarate aminotransferase (Ala AT, EC 2.6.1.2) from plants and animals, and the mRNA of this clone increased 4- to 5-fold 4 h after cells were switched from high-CO2 to low-CO2 growth conditions. The expression of the enzyme and its activity also increased accordingly at low-CO2 growth conditions. To study the physiological role of Ala AT, a pyridoxal phosphate inhibitor, aminooxyacetic acid, was added at 40 microM to the growth medium when cells were beginning to adapt to low CO2. This caused a 30% decrease in the maximum photosynthetic rate in air-adapting cells 8 h later. The addition of the inhibitor also caused the cells to excrete glycolate, a photorespiratory intermediate, but did not change the apparent affinity of the cell for external CO2. These physiological studies are consistent with the assumption that Ala AT is involved in the adaptation to low-CO2 conditions.
Chen
Z Y
ZY
Department of Plant Biology, Louisiana State University, Baton Rouge 70803, USA.
Burow
M D
MD
Mason
C B
CB
Moroney
J V
JV
D000220:Adaptation, Biological; D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002245:Carbon Dioxide / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005784:Gene Amplification; D015723:Gene Library; D008969:Molecular Sequence Data; D010788:Photosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D002245:Carbon Dioxide; D000625:Aminooxyacetic Acid; D000410:Alanine Transaminase