title: Expression of jumper ant (Myrmecia pilosula) venom allergens: post-translational processing of allergen gene products.
abstract: N-terminal analyses of electrophoretically-separated allergenic polypeptides of the venom of the jumper ant M. pilosula showed that five out of the six allergenic polypeptides identified are homologous with the cloned major allergen Myr p I and may be derived from a single precursor polypeptide. The sixth polypeptide is homologous with a second cloned major allergen, Myr p II which is expressed as a single precursor polypeptide but exists in its native form as a disulphide bond-linked complex.
authors.affiliation: Molecular Immunology Unit, Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards NSW, Australia.
authors.forename: G R
authors.initials: GR
authors.lastname: Donovan
authors.forename: M D
authors.initials: MD
authors.lastname: Street
authors.forename: T
authors.initials: T
authors.lastname: Tetaz
authors.forename: A I
authors.initials: AI
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Alewood
authors.forename: P
authors.initials: P
authors.lastname: Alewood
authors.forename: S K
authors.initials: SK
authors.lastname: Sutherland
authors.forename: B A
authors.initials: BA
authors.lastname: Baldo
mesh_terms: D000478:Alkylation; D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D000862:Ant Venoms / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D001000:Ants / Q000235:genetics*; D018076:DNA, Complementary / Q000276:immunology; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015151:Immunoblotting; D019476:Insect Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid
chemical_list: D000485:Allergens; D000862:Ant Venoms; D018076:DNA, Complementary; D004220:Disulfides; D019476:Insect Proteins; C079435:Myr p I; C096132:Myr p II protein, Myrmecia pilosula; D011498:Protein Precursors