Impairment of vitamin A uptake by rat hepatocytes and fat storing cells determined by Monensin--morphological observations.
The action of the Na+/H+ antiport monensin on vitamin A uptake by rat liver has been studied in vivo. The quickfading autofluorescence of vitamin A has been used for the determination of vitamin A uptake by the liver. Pretreatment of rats intraperitoneally with monensin decreases the uptake of vitamin A by hepatocytes and its transfer for storage to fat storing cells. Pretreatment of rats intraperitoneally with vitamin A for a short time, then with monensin, shows that the hepatocytes no longer transfer vitamin A to fat storing cells for storage. These results might indicate that monensin impairs the uptake of vitamin A by the hepatocytes and might also impair the transport of vitamin A from parenchymal to perisinusoidal cells.
Novelli
A
A
Institute of General Pathology, University of Genoa, Italy.
Gambella
G
G
Casu
A
A
Paltrinieri
F
F
Persi
A
A
Canepa
M
M
Carta
L
L
Nanni
G
G
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008985:Monensin / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014801:Vitamin A / Q000037:antagonists & inhibitors* / Q000378:metabolism*
D014801:Vitamin A; D008985:Monensin