title: Evaluation of cyclic AMP accumulation in EBV-transformed human B-lymphocytes: effects of dopamine agonists, isoproterenol, prostaglandin E1, cholera toxin, forskolin, and phorbol 12-myristate-13 acetate.
abstract: 1. Phorbol 12-myristate-13-acetate (PMA), a protein kinase C activator, elevated cyclic AMP accumulation in EBV-transformed human B-lymphocytes, and potentiated isoproterenol-, prostaglandin- (PGE1), cholera toxin-, and forskolin-stimulated cyclic AMP accumulation. 2. The dopamine D1 receptor agonist, SKF38393 (10(-7) to 10(-5) MH, had no effect on cyclic AMP accumulation in transformed human B-lymphocytes. 3. The dopamine D2 receptor agonist, quinpirole (10(-7) to 10(-5) MH did not inhibit cyclic AMP accumulation even when cyclic AMP accumulation was maximized by the addition of PMA and forskolin. 4. These data suggest that dopamine D1- and D2-receptor coupling to a cyclic AMP generating system is not present at detectable levels in transformed human B-lymphocytes.
authors.affiliation: National Institute of Mental Health, Neuroscience Center at Saint Elizabeth Hospital, Neuropsychiatry Branch, Washington, D.C., USA.
authors.forename: N
authors.initials: N
authors.lastname: Natsukari
authors.forename: H
authors.initials: H
authors.lastname: Kulaga
authors.forename: I
authors.initials: I
authors.lastname: Baker
authors.forename: R J
authors.initials: RJ
authors.lastname: Wyatt
authors.forename: J M
authors.initials: JM
authors.lastname: Masserano
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects*; D002772:Cholera Toxin / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology
chemical_list: D018491:Dopamine Agonists; D010703:Phorbol Esters; D005576:Colforsin; C008874:phorbol-12,13-diacetate; D002772:Cholera Toxin; D000242:Cyclic AMP; D000527:Alprostadil; D007545:Isoproterenol