Purification of a lipase from Acinetobacter calcoaceticus AAC323-1 by hydrophobic-interaction methods.
The performance of hydrophobic-interaction chromatography (HIC) for the purification of Acinetobacter calcoaceticus AAC323-1 lipase was compared with that of various aqueous two-phase systems. While a 42% lipase yield with a purification factor of 140 could be recovered by HIC, higher yields were achieved by using aqueous two-phase systems, either those formed by poly(ethylene glycol) and dextran or those based upon the use of a detergent. Triton X-114-based aqueous two-phase partition showed the best performance, with a yield of 81% and a purification factor of 68. Further detergent removal was easily achieved with an adsorbent, with no significant decrease in yields. Owing to its simplicity, the method should be easy to scale-up.
Bompensieri
S
S
Cátedra de Microbiología Industrial y Biotecnología, Universidad de Buenos Aires, Argentina.
Gonzalez
R
R
Kok
R
R
Miranda
M V
MV
Nutgeren-Roodzant
I
I
Hellingwerf
K J
KJ
Cascone
O
O
Nudel
B C
BC
D016954:Acinetobacter calcoaceticus / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008049:Lipase / Q000302:isolation & purification*; D012995:Solubility; D014867:Water / Q000737:chemistry
D014867:Water; D008049:Lipase