[CAL 54, a new cell line derived from a human renal carcinoma: characterization and radiosensitivity].
A new cell line (CAL 54) was isolated from a malignant pleural effusion of a patient with renal carcinoma. CAL 54 is a continuous and stable cell line. Immunochemical staining showed simultaneous expression of cytokeratin, epithelial membrane antigen and vimentin. Cytometric flow analysis of DNA content reveals one major hyperdiploid population. Histological aspect of the tumor induced in the nude mouse showed well differentiated adenocarcinoma with papillary structure. Radiation response of these cells was evaluated by the colony-forming method and the data were fitted with the linear-quadratic model. Survival at 2 Gy (SF2) was 0.28 and the mean inactivation dose (D) = 1.50 Gy, ranking this cell line among the radiation sensitive cells. CAL 54 may be an informative cell line to investigate radiation effects in the management of renal tumours.
Gioanni
J
J
Centre Antoine-Lacassagne, Nice, France.
Zanghellini
E
E
Mazeau
C
C
Amiel
J
J
Poustis-Delpont
C
C
Lagrange
J L
JL
Duplay
H
H
Caldani
C
C
Courdi
A
A
D000368:Aged; D000818:Animals; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010997:Pleural Neoplasms / Q000473:pathology / Q000556:secondary; D014407:Tumor Cells, Cultured