title: In vivo monitoring of .OH generation on jejunal ischemic injury by dialysis technique.
abstract: We applied an in vivo microdialysis technique to examine free radical generation following ischemic injury in the rat jejunum. For this purpose, we used a microdialysis probe holding system. The salicylate reacts with hydroxyl free radical (.OH) and generates 2,3-dihydroxybenzoic acid (DHBA), which can be measured electrochemically in picomole amounts by high-performance liquid chromatography (HPLC). Therefore, the former was used for trapping the .OH formation. Reperfusion of the jejunum produced progressive elevations in the levels of 2,3-DHBA achieving the maximums at 20 min, after which they decreased progressively to the baseline. These elevations were significant only after 10 min- and 20 min-ischemia. However, this change was not significant in the case of 30 min-ischemia. Ischemia-reperfusion of rat intestine caused morphological changes. These results suggest that .OH production is significantly reduced after 30 min-ischemia providing thus the possibility for more severe intestinal damage. In conclusion our dialysis probe can be useful for the measurement of .OH generation following ischemia-reperfusion injury.
authors.affiliation: Department of Surgery II, Oita Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirata
authors.forename: T
authors.initials: T
authors.lastname: Obata
authors.forename: Y
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Uchida
mesh_terms: D000818:Animals; D062385:Hydroxybenzoates / Q000032:analysis; D017665:Hydroxyl Radical / Q000032:analysis*; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D007583:Jejunum / Q000098:blood supply*; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D062385:Hydroxybenzoates; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid