title: Osteolytic properties of the synovial-like tissue from aseptically failed joint prostheses.
abstract: Relationships were found between the bone-resorbing ability of conditioned media (CMs) from culture of peri-prosthetic tissues and their levels of bone-remodelling agents. Bone-resorbing activity was measured by 45Ca release from pre-labelled mouse calvaria and 23 of 40 CMs exhibited bone-resorbing activity. Cytokine and prostanoid levels in the CMs were measured by immunoassay, and the levels of interleukin (IL)-1 beta, IL-6, tumour necrosis factor alpha (TNF alpha) and prostaglandin E2 (PGE2) correlated with each other, except for the latter two. Significantly higher levels of IL-6 were present in those CMs with bone-resorbing activity than in those without, and a similar pattern was observed for PGE2 and IL-1 beta. However, some CMs with high levels of IL-1 beta, IL-6, TNF alpha and PGE2 failed to induce resorption, whereas a few CMs with low levels of these agents induced resorption. Moreover, neither dialysis of CMs nor addition of neutralizing antisera to IL-1 alpha and IL-1 beta to CMs, either alone or in combination, reduced the bone-resorbing activity of the CMs. It is considered that these agents may act synergistically to mediate osteolysis around failed joint implants, but that other unidentified bone-resorbing agent(s) must be involved.
authors.affiliation: Department of Pathology, University of Bristol.
authors.forename: M J
authors.initials: MJ
authors.lastname: Perry
authors.forename: F M
authors.initials: FM
authors.lastname: Ponsford
authors.forename: F Y
authors.initials: FY
authors.lastname: Mortuza
authors.forename: I D
authors.initials: ID
authors.lastname: Learmonth
authors.forename: R M
authors.initials: RM
authors.lastname: Atkins
authors.forename: C J
authors.initials: CJ
authors.lastname: Elson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001207:Asepsis; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003956:Dialysis; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology; D007595:Joint Prosthesis; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D009924:Organ Culture Techniques; D010014:Osteolysis / Q000139:chemically induced / Q000503:physiopathology*; D011475:Prosthesis Failure; D013583:Synovial Membrane / Q000503:physiopathology*
chemical_list: D015415:Biomarkers; D002132:Calcium Radioisotopes; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D015232:Dinoprostone; D002118:Calcium