NASBA: a novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements.
Although immunoassays have long served as the standard in the field of diagnostics, the advent of nucleic acid amplification technologies allows for a new array of diagnostic applications. NASBA, nucleic acid sequence-based amplification, is one such technology that is highly suited for the amplification of RNA. As such, NASBA is applied readily as a diagnostic tool for infectious diseases, particularly for RNA viruses, such as retroviruses. The development and application of NASBA technology as a qualitative and quantitative diagnostic system for HIV-1 are described in this article.
Romano
J W
JW
Department of Cell Biology, Advanced BioScience Laboratories, Inc., Kensington, Maryland, 20855, USA.
van Gemen
B
B
Kievits
T
T
D015658:HIV Infections / Q000175:diagnosis / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*
D012367:RNA, Viral