title: Comparison of 2 centrifugation procedures in the formalin-ethyl acetate stool concentration technique for the detection of Cryptosporidium oocysts.
abstract: A comparison between the standard formalin-ethyl acetate (FEA) stool concentration procedure (centrifugation at 400 g for 2 min) and a modification proposed to increase the recovery of Cryptosporidium oocysts (500 g for 10 min) was performed on 73 formalin-preserved stool specimens known to be positive for Cryptosporidium. False-negative results were obtained with both methods, but the increase in centrifugation time and force resulted in the detection of a higher number of oocysts and positive samples. The modified procedure had a sensitivity significantly higher than the standard method (99% compared to 86%, P = 0.0045).
authors.affiliation: Servicio de Microbiología y Parasitología, Hospital Clínico Universitario, Zaragoza, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Clavel
authors.forename: A
authors.initials: A
authors.lastname: Arnal
authors.forename: E
authors.initials: E
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Varea
authors.forename: J
authors.initials: J
authors.lastname: Quílez
authors.forename: I
authors.initials: I
authors.lastname: Ramírez
authors.forename: F J
authors.initials: FJ
authors.lastname: Castillo
mesh_terms: D000085:Acetates; D000818:Animals; D002498:Centrifugation; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005557:Formaldehyde; D006801:Humans; D010274:Parasitology / Q000379:methods*; D015053:Zygote
chemical_list: D000085:Acetates; D005557:Formaldehyde; C007650:ethyl acetate