Cytokine expression in the liver of mice infected with a highly virulent strain of Francisella tularensis.
Cytokine mRNA expression was determined in the liver of mice subcutaneously inoculated with a lethal dose of the highly virulent strain FSC 041 of Francisella tularensis subvar, tularensis or a sublethal dose of the live vaccine strain of F. tularensis subvar. palaearctica. Expression of mRNA for TNF-alpha, IL-12, IFN-gamma, and IL-10 was demonstrated within 48 h of inoculation, the kinetics being similar irrespective of bacterial strain used. Thus, the expression of a cytokine response believed to be important in the early host defence against live vaccine strain seemed insufficient to prevent the lethality of a more virulent strain.
Golovliov
I
I
Department of Microbiology, National Defence Research Establishment, University of Umeå, Sweden.
Kuoppa
K
K
Sjöstedt
A
A
Tärnvik
A
A
Sandström
G
G
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005604:Francisella tularensis / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity*; D008099:Liver / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis; D013045:Species Specificity; D014406:Tularemia / Q000276:immunology*; D014774:Virulence / Q000276:immunology
D016207:Cytokines; D012333:RNA, Messenger