title: The determination of the extracellular concentration of brain glutamate using quantitative microdialysis.
abstract: Quantitative microdialysis with two enzyme-based assays was used to determine the extracellular concentration of glutamate in the striatum of freely moving rats. From the difference between infused and dialysate glutamate a value of 3.0 +/- 0.6 microM for the extracellular glutamate concentration was computed by regression analysis. The in vivo recovery, derived from the slope of the regression line, was 50%.
authors.affiliation: University Laboratory of Physiology, Oxford, UK.
authors.forename: M
authors.initials: M
authors.lastname: Miele
authors.forename: M
authors.initials: M
authors.lastname: Berners
authors.forename: M G
authors.initials: MG
authors.lastname: Boutelle
authors.forename: H
authors.initials: H
authors.lastname: Kusakabe
authors.forename: M
authors.initials: M
authors.lastname: Fillenz
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018698:Glutamic Acid