Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells.
Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; there-after, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.
Borboni
P
P
Department of Internal Medicine, University of Rome Tor Vergata, Italy.
Magnaterra
R
R
Rabini
R A
RA
Staffolani
R
R
Porzio
O
O
Sesti
G
G
Fusco
A
A
Mazzanti
L
L
Lauro
R
R
Marlier
L N
LN
D000818:Animals; D001483:Base Sequence; D001710:Biotin / Q000494:pharmacology*; D002478:Cells, Cultured; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005941:Glucokinase / Q000235:genetics* / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013997:Time Factors
D017931:DNA Primers; D007328:Insulin; D012333:RNA, Messenger; D001710:Biotin; D005941:Glucokinase