Cloning and characterization of transcriptional promoters from Bacillus subtilis phage 2C.
Phage 2C is a Bacillus subtilis lytic phage, whose genome contains hydroxymethyluracil in place of thymine. To isolate promoters of early phage genes involved in the take-over of cellular metabolism, 2C DNA libraries were constructed in promoter-probe plasmids replicating in Escherichia coli and B. subtilis. Four different 2C DNA fragments strongly expressed reporter genes in E. coli but not in B. subtilis. All fragments originated from unique sequences of the genome and not from its terminal redundancies. One fragment was sequenced. Despite the presence of an sigma-A-RNA polymerase binding site upstream of the transcriptional initiation site of a 2C early gene, this fragment did not promote transcription in B. subtilis.
Daxhelet
G
G
Microbial Pathogenesis Unit, Medical School, University of Louvain, Brussels, Belgium.
Gilot
P
P
Hoet
P
P
D017103:Bacillus Phages / Q000235:genetics*; D001412:Bacillus subtilis / Q000821:virology*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017781:Genes, Immediate-Early / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases