Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus.
Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein interact with the V proteins. We examined the interaction of the NP proteins and the P, V proteins or deletion mutants of V protein using immunofluorescence and co-immunoprecipitation plus Western blotting analyses, and showed that the V proteins of HPIV-2 bind to the NP proteins and that the N-terminal domain of V protein interacts directly with the NP proteins. When the NP proteins were co-expressed with the V proteins or the N-terminal fragments (aa 1-46), the NP proteins were detected diffusely in the nuclei of the transfected cells, and were also detected in cytoplasmic inclusions. The NP and V proteins were co-localized in the nuclei or cytoplasm. Furthermore, the NP proteins were co-precipitated with the P, V, and V (1-164) proteins by a specific antibody. The P proteins interact more closely with the NP proteins than do the V proteins. These findings indicate that the V proteins have the ability to bind the NP proteins.
Watanabe
N
N
Department of Microbiology, Mie University School of Medicine, Japan.
Kawano
M
M
Tsurudome
M
M
Nishio
M
M
Ito
M
M
Ohgimoto
S
S
Suga
S
S
Komada
H
H
Ito
Y
Y
D001665:Binding Sites; D001692:Biological Transport; D002451:Cell Compartmentation; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007181:Inclusion Bodies, Viral; D009154:Mutation; D010223:Parainfluenza Virus 2, Human / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019213:Rubulavirus / Q000235:genetics; D017384:Sequence Deletion; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins