Determination of ester-type local anesthetic drugs (procaine, tetracaine, and T-caine) in human serum by wide-bore capillary gas chromatography with nitrogen-phosphorus detection.
A sensitive method for simultaneous determination of ester-type local anesthetic drugs (procaine, tetracaine, and T-caine) has been developed using wide-bore capillary gas chromatography with nitrogen-phosphorus detection (GC-NPD). The extraction procedure, the experimental conditions for heptafluorobutyryl (HFB) derivative formation, and the percentage of the ester-type local anesthetic drugs from the human serum are described. The HFB derivatives of ester-type local anesthetic drugs showed sensitivity of approximately 2-3 fold higher than that without derivatization. The detection limits of HFB derivatives of the ester-type local anesthetic drugs were approximately 60-70 pg on column. Recoveries from the human serum were 85-94%. This method could be used to determine concentrations as low as 24-28 ng/mliters of the ester-type local anesthetic drugs.
Terada
M
M
Department of Legal Medicine, Osaka University Medical School, Japan.
Islam
M N
MN
Tun
Z
Z
Honda
K
K
Wakasugi
C
C
Shinozuka
T
T
Yanagida
J
J
Yamamoto
T
T
Kuroiwa
Y
Y
D010129:4-Aminobenzoic Acid / Q000032:analysis / Q000097:blood; D000779:Anesthetics, Local / Q000032:analysis* / Q000097:blood; D006801:Humans; D009584:Nitrogen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D011343:Procaine / Q000032:analysis* / Q000097:blood; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013748:Tetracaine / Q000032:analysis* / Q000097:blood; D062366:para-Aminobenzoates
D000779:Anesthetics, Local; D012997:Solvents; D062366:para-Aminobenzoates; D013748:Tetracaine; C104401:p-(butylamino)benzoic acid-2-(diethylamino)ethyl ester; D010758:Phosphorus; D011343:Procaine; D009584:Nitrogen; D010129:4-Aminobenzoic Acid