title: Detection of Escherichia coli O157:H7 in water from coliform enrichment cultures.
abstract: Environmental water samples were seeded with Escherichia coli O157:H7 and the bacterium was recovered using a traditional coliform enrichment procedure followed by selective plating on sorbitol MacConkey agar and biochemical and serological characterization. Assays for beta-glucuronidase and glutamate decarboxylase were found to be useful procedures for screening suspected isolates. The organism was not recovered in a survey of various water samples.
authors.affiliation: US Environmental Protection Agency, Cincinnati, OH 45268, USA.
authors.forename: E W
authors.initials: EW
authors.lastname: Rice
authors.forename: C H
authors.initials: CH
authors.lastname: Johnson
authors.forename: D J
authors.initials: DJ
authors.lastname: Reasoner
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005966:Glucuronidase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D012703:Serotyping; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D005966:Glucuronidase; D005968:Glutamate Decarboxylase