Synaptic organization of an organotypic slice culture of the mammalian retina.
Vertical slices of postnatal day 6 (P6) rat retina were cut and cultured using the roller-tube technique. The organotypic differentiation during a culture period of up to 30 days has been described in a previous study (Feigenspan et al., 1993a). Here we concentrated on the synaptic organization in the retinal slice culture. Electron microscopy revealed the presence of ribbon synapses in the outer plexiform layer and conventional and ribbon synapses in the inner plexiform layer. Immunofluorescence with antibodies that recognize specific subunits of GABAA or glycine receptors revealed a punctate distribution of the receptors. They were aggregated in "hot spots" that correspond to a concentration of receptors at postsynaptic sites. Different isoforms of GABAA and glycine receptors occurred in the slice cultures. The experiments show that there is a differentiation of synapses and a diversity of transmitter receptors in the slice cultures that is comparable to the in vivo retina.
Sassoè-Pognetto
M
M
Max-Planck-Institut für Hirnforschung, Neuroanatomische Abteilung, Frankfurt, Germany.
Feigenspan
A
A
Bormann
J
J
Wässle
H
H
D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000378:metabolism; D018009:Receptors, Glycine / Q000378:metabolism; D012160:Retina / Q000378:metabolism / Q000648:ultrastructure*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure*; D009435:Synaptic Transmission / Q000502:physiology*
D002352:Carrier Proteins; D008565:Membrane Proteins; D018079:Receptors, GABA; D018009:Receptors, Glycine; C073441:gephyrin