Characteristics of shed snake skin permeability to indomethacin and fatty alcohols.
To investigate the utilities of a shed snake skin as a model membrane for preclinical studies of transdermal drug delivery, the flux of indomethacin was determined under various conditions by using a diffusion cell. The flux of fatty alcohols was determined and compared with that in human skin reported in references. The esterase activity of shed snake skin was also determined. It was found that the flux of indomethacin decreased with an increase of pH and the amount of ethanol in a vehicle. The flux of indomethacin increased by the addition of Azone, N-methyl-2-pyrrolidone and N,N-dimethyl-m-toluamide in the cream. The flux of fatty alcohols in shed snake skin was greater than that reported in human skin, and shed snake skin had similar esterase activity to human skin.
Kuramoto
M
M
Pharmaceutical Research Laboratories, Dainippon Pharmaceutical Co. Ltd, Osaka, Japan.
Tanaka
T
T
Makita
H
H
Nakamura
Y
Y
Yata
N
N
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017815:Elapidae; D004950:Esterases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005233:Fatty Alcohols / Q000378:metabolism*; D007213:Indomethacin / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D012911:Snakes
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D005233:Fatty Alcohols; D000431:Ethanol; D004950:Esterases; D007213:Indomethacin