Influence of Marek's disease virus strain AC-1 on cellular immunity in birds carrying endogenous viral genes.
The effects of Marek's disease virus (MDV) strain AC-1 on humoral and cellular immune responses were investigated in two lines of chickens segregating for the endogenous viral (ev) genes ev-6 and ev-12. All birds were vaccinated at 14 days of age against Newcastle disease virus (NDV). At 3 wk of age, 48 birds per line received an intraperitoneal injection of MDV (AC-1 isolate), and 24 were injected with saline. Birds of each group were killed on days 5, 7, 12, and 14 postinfection. Data were first analyzed for each day of testing. Results showed that, for variable measured, treatment effects were similar on days 5 and 7, and on days 12 and 14. Therefore, day 5 and day 7 data, and day 12 and day 14 data were pooled and analyzed. In MDV-infected chickens, proliferative lymphocyte responses to mitogens were suppressed (P < 0.001) after the first and second week of infection, whereas responses to NDV antigen were enhanced (P < 0.001) after the first week and then reduced (P < 0.01) by the end of the second week when compared to uninfected birds. The percentage of CD4+ T cells was higher (P < 0.01), and the percentage of cells expressing major histocompatibility complex (MHC) class II antigens was lower (P < 0.001), in MDV-infected chickens than in uninfected birds. The cytotoxic activity of natural killer (NK) cells was also enhanced (P < 0.001) in MDV-infected birds when compared to uninfected birds. Antibody responses to NDV were not different among groups, and the presence of ev-6 or ev-12 genes did not influence the immune response parameters measured in both infected and uninfected chickens. In conclusion, a marked increase in the CD4+ T lymphocyte population occurred in the early stage of MDV infection in all chickens regardless of the presence of ev genes, whereas the number of cells expressing MHC class II antigen was severely reduced.
Lessard
M
M
Centre for Food and Animal Research, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
Hutchings
D L
DL
Spencer
J L
JL
Lillehoj
H S
HS
Gavora
J S
JS
D000818:Animals; D000917:Antibody Formation; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005814:Genes, Viral / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology; D008380:Marek Disease / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D014765:Viral Vaccines / Q000276:immunology
D014765:Viral Vaccines