Involvement of antipain-sensitive protease activity in the interferon-beta-induced UV-refractoriness of Cockayne syndrome fibroblasts.
Fibroblast cells obtained from two siblings and a female patient with Cockayne syndrome (CS), when pretreated with human interferon (HuIFN)-beta prior to irradiation with UV light (254 nm wavelength), exhibited transiently induced fibrinolytic protease activity immediately after the irradiation in association with increased refractoriness to UV cell-killing. A protease inhibitor, antipain, inhibited the induction of protease activity in lysates of the CS fibroblasts from these 3 cases after the combination of HuIFN-beta pretreatment and UV irradiation, whereas elastatinal and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) inhibited the activity less than antipain did. Antipain also suppressed the increase in UV-refractoriness of HuIFN-beta-pretreated CS fibroblasts, as revealed by culturing cells for 24 h in medium containing the inhibitor immediately after UV exposure and thereafter evaluating the ability of colony formation by the cells. Thus, an antipain-sensitive protease may be involved in the UV-refractoriness induced by HuIFN-beta in CS fibroblast strains.
Sugita
K
K
Department of Pediatrics, Faculty of Medicine, University of Chiba, Japan.
Suzuki
N
N
Niimi
H
H
D000976:Antipain / Q000494:pharmacology; D002470:Cell Survival / Q000528:radiation effects; D003057:Cockayne Syndrome / Q000235:genetics*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000502:physiology*; D008297:Male; D010375:Pedigree; D011480:Protease Inhibitors / Q000494:pharmacology; D014466:Ultraviolet Rays
D011480:Protease Inhibitors; D000976:Antipain; D016899:Interferon-beta; D010450:Endopeptidases