title: Nutrient uptake and culture of Sminthopsis macroura (stripe-faced dunnart) embryos.
abstract: Glucose and pyruvate uptake by individual embryos were measured in a marsupial species (stripe-faced dunnart) and a eutherian species (mouse). At each stage of development, nutrient uptake by the dunnart embryo was around an order of magnitude greater than that of the mouse embryo. The pattern of glucose uptake by the dunnart embryo was not like that for any eutherian embryo, all of which have a low glucose uptake before the blastocyst stage. Rather, in the dunnart embryo there was a significant increase in glucose uptake after the third cleavage division, increasing from 13.6 pmol embryo h-1 at the 4-cell stage to 34.9 pmol embryo h-1 by the 8-cell stage. This increase in glucose uptake before blastocyst formation may be attributed to an increased energy demand associated with the movement of cells within the dunnart embryo. Using a new culture system, it was possible to culture 66% of dunnart embryos at the 2-4-cell stage and 80% of those at the 8-16-cell stage to the unilaminar blastocyst stage. Embryos cultured from the 2-cell to the 4-cell stage were retarded by around 12 h when they reached the blastocyst stage. Developmental retardation was also reflected in the pattern of nutrient uptake, which lagged behind that of embryos developed in vivo. The present study has shown that it is possible to culture the early marsupial embryo to the blastocyst stage in a serum-free culture system, while concomitantly quantifying embryonic nutrient requirements. Such an approach is essential for species where there is a paucity of material for study.
authors.affiliation: Human and Animal Reproductive Biology Group, Monash University, Clayton, Vic, Australia.
authors.forename: D K
authors.initials: DK
authors.lastname: Gardner
authors.forename: L
authors.initials: L
authors.lastname: Selwood
authors.forename: M
authors.initials: M
authors.lastname: Lane
mesh_terms: D000818:Animals; D001692:Biological Transport; D001755:Blastocyst / Q000378:metabolism; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008394:Marsupialia / Q000196:embryology*; D051379:Mice; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D019289:Pyruvic Acid; D005947:Glucose