Comparison of the effects of bradykinin and related compounds on isolated mouse and human uterus.
The purpose of this study was to investigate and compare the response of the isolated human myometrium (non-pregnant donors) and mouse uterus to bradykinin (BK), Lys-BK and des-Arg9-BK (+/-2.79 microM indomethacin). The uterine strips were set up for superfusion using Kerbs' solution. On the human myometrium the responses to BK and Lys-BK were biphasic and consisted of an increase in myometrial tension which was followed by a period of inhibition of myogenic activity. Des-Arg9-BK evoked a monophasic contractile response. On the mouse uterus the responses to BK, Lys-BK and des-Arg9-BK were monophasic and contractile only. On both of the tissues the contractile responses to BK and Lys-BK were bell shaped and indomethacin abolished the bell-shaped part of the dose response curves. The response to des-Arg9-BK and the inhibitory response to BK and Lys-BK, on the human tissue, was also significantly reduced in the presence of indomethacin. The results of this study suggest that the human and mouse uterus do posses kinin receptors of the B2 type but on human myometrium these are biphasic responses.
Abbas
F
F
Department of Pharmacology, University of Bradford, West Yorkshire, UK.
Clayton
J
J
Marshall
K
K
Scott
H
H
Senior
J
J
D000818:Animals; D001920:Bradykinin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007609:Kallidin / Q000494:pharmacology; D051379:Mice; D013045:Species Specificity; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects*
C024626:bradykinin, des-Arg(9)-; D007609:Kallidin; D001920:Bradykinin; D007213:Indomethacin