title: Detection of herpesvirus-like DNA in the bronchoalveolar lavage fluid of patients with pulmonary Kaposi's sarcoma.
abstract: Pulmonary involvement is a clinically important form of visceral Kaposi's sarcoma in immunocompromised patients. Recently, herpesvirus-like deoxyribonucleic acid (DNA) sequences, defining a new herpesvirus termed "human herpesvirus 8" (HHV8) or "Kaposi's sarcoma-associated herpesvirus" (KSHV), were detected in Kaposi's sarcoma of acquired immune deficiency syndrome (AIDS) and non-AIDS patients. We describe the successful detection of HHV8 DNA in the bronchoalveolar lavage (BAL) fluid of patients with pulmonary Kaposi's sarcoma. Three immunocompromised patients, two HIV seropositive and one after kidney transplantation, suffered from respiratory symptoms and showed pulmonary infiltrates on chest radiography after development of biopsy proven Kaposi's sarcoma of the skin. Bronchoscopy revealed the typical Kaposi like livid endobronchial lesions. BAL fluid was analysed for the presence of HHV8 DNA using a nested polymerase chain reaction (PCR) assay. HHV8 DNA was detected in the BAL fluid of all three patients. In addition, HHV8 DNA could be detected in the skin biopsy tissue, lymph node, and peripheral blood mononuclear cells of these patients. Our data show that human herpesvirus 8 deoxyribonucleic acid can be detected in the bronchoalveolar lavage fluid of patients with pulmonary Kaposi's sarcoma. If further studies reveal a high specificity for human herpesvirus 8 deoxyribonucleic acid detection, this test will improve the tools for the diagnosis of pulmonary Kaposi's sarcoma without further need of biopsies.
authors.affiliation: Institute for Pathology, University of Basel, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Cathomas
authors.forename: M
authors.initials: M
authors.lastname: Tamm
authors.forename: C E
authors.initials: CE
authors.lastname: McGandy
authors.forename: A P
authors.initials: AP
authors.lastname: Perruchoud
authors.forename: M J
authors.initials: MJ
authors.lastname: Mihatsch
authors.forename: P
authors.initials: P
authors.lastname: Dalquen
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral