Joint fluid carboxy-terminal type II procollagen peptide as a marker of cartilage collagen biosynthesis.
Joint fluid levels of carboxy-terminal type II procollagen peptide (pCOL II-C) were measured in osteoarthritis, rheumatoid arthritis and traumatic arthritis by a newly developed one-step enzyme immunoassay (EIA). The detection limit of the new method was as low as 0.2 ng/ml. The levels of pCOL II-C were significantly (P < 0.001) higher in osteoarthritis and traumatic arthritis than in rheumatoid arthritis. In osteoarthritis, pCOL II-C levels were higher in moderately afflicted patients. Since type II collagen is a unique component of cartilage, pCOL II-C levels in joint fluids could reflect the synthetic activity of type II collagen of chondrocytes in the diseased joint and therefore could be utilized as a simple marker of type II collagen synthesis in articular cartilage in joint diseases.
Shinmei
M
M
Department of Orthopaedic Surgery, National Defense Medical College, Tokorozawa, Japan.
Ito
K
K
Matsuyama
S
S
Yoshihara
Y
Y
Matsuzawa
K
K
D000328:Adult; D000368:Aged; D001168:Arthritis / Q000209:etiology / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D015415:Biomarkers; D002358:Cartilage, Articular / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007718:Knee Injuries / Q000150:complications; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism*; D011347:Procollagen / Q000378:metabolism*; D015203:Reproducibility of Results; D013582:Synovial Fluid / Q000378:metabolism*
D015415:Biomarkers; D010446:Peptide Fragments; D011347:Procollagen; C093212:procollagen type II carboxy-terminal peptide; D003094:Collagen