title: Modulation of human papillomavirus type 16 mRNA in cervical invasive carcinoma patients by interferon-alpha therapy.
abstract: Mechanisms by which interferon produces papilloma regression remain largely unknown. We analyzed biopsies from three cervical invasive carcinoma patients treated with interferon-alpha (IFN-alpha) administered both topically and i.m. for 15 days. All specimens contained human papillomavirus (HPV-16) DNA as determined by polymerase chain reaction using specific HPV-16 E7 primers. Interestingly, in two patients. HPV-16 mRNA expression was reduced (44% and 67%, respectively) after IFN treatment. Upregulation of 2-5 A synthetase and PKR mRNA levels were indicative of the IFN effect. A larger study should be initiated to see whether IFN-alpha modulates the HPV-16 mRNA levels in tumor biopsies from cervical carcinoma patients.
authors.affiliation: Departamento de Biologia Celular, Centro de Investigaciones Biologicas, Ciudad Habana, Cuba.
authors.forename: R
authors.initials: R
authors.lastname: García-Milián
authors.forename: M A
authors.initials: MA
authors.lastname: Rios
authors.forename: M
authors.initials: M
authors.lastname: Amigó
authors.forename: D
authors.initials: D
authors.lastname: Díaz
authors.forename: O
authors.initials: O
authors.lastname: Guilar
authors.forename: M
authors.initials: M
authors.lastname: Silveira
authors.forename: M J
authors.initials: MJ
authors.lastname: Araña
authors.forename: S E
authors.initials: SE
authors.lastname: Perea
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use*; D009361:Neoplasm Invasiveness; D027383:Papillomaviridae / Q000235:genetics*; D010865:Pilot Projects; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000821:virology
chemical_list: D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012333:RNA, Messenger; D011994:Recombinant Proteins