Molecular cloning of two novel rab genes from human melanocytes.
We isolated the genes of two small GTP-binding proteins of the rab family from a human melanocyte cDNA library and from melanoma cells. One gene, rab30 codes for a novel rab protein of 203 amino acids with minimal homology to previously documented GTPases. The other, rab22b, appears to be an isoform of the human homologue of canine rab22. Both rab mRNAs displayed a nearly ubiquitous pattern of expression in the various tissues examined. Rab22b and rab30 were mapped to chromosomes 18 and 11, respectively.
Chen
D
D
Section on Human Biochemical Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1830, USA.
Guo
J
J
Miki
T
T
Tachibana
M
M
Gahl
W A
WA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004285:Dogs; D020558:GTP Phosphohydrolases / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D008544:Melanocytes / Q000737:chemistry*; D008545:Melanoma / Q000235:genetics*; D008969:Molecular Sequence Data; D017385:Sequence Homology; D014407:Tumor Cells, Cultured; D020691:rab GTP-Binding Proteins
C484191:RAB22A protein, human; C484190:RAB31 protein, human; C086223:rab22 protein, Canis familiaris; C101860:RAB30 protein, human; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins