Expression of CD26 does not correlate with the replication of macrophage-tropic strains of HIV-1 in T-cell lines.
Strains of human immunodeficiency virus type 1 (HIV-1) differ significantly in both genetic content and biological properties. One of the earliest discovered differences between HIV-1 strains was divergence in the relative ability of different strains to replicate in either T-cell lines or monocytes/macrophages. This observation has led to the suggestion that molecules present on the surface of HIV-susceptible cells other than CD4 may interact with gp120 in facilitating the entry of HIV-1 into host cell populations. Several reports have suggested that CD26, a cell surface protease expressed on many cells of the immune system including some CD4+ T-cells and macrophage, may be an accessory molecule for HIV-1 entry. Recently, it has also been reported that the expression of high levels of CD26 correlates with the entry and replication of macrophage-tropic strains of HIV-1 in a T-cell line. In this report, we demonstrate that replication of macrophage-tropic strains of HIV-1 in T-cell lines is independent of CD26 expression. From this observation, we conclude that CD26 plays no role in the entry of HIV-1 into these cells.
Watkins
B A
BA
Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA.
Crowley
R W
RW
Davis
A E
AE
Louie
A T
AT
Reitz
M S
MS
D000818:Animals; D019556:COS Cells; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis / Q000276:immunology*; D005786:Gene Expression Regulation; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000821:virology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D016655:HIV Core Protein p24; D018819:Dipeptidyl Peptidase 4