title: Comparison of immunosuppressive properties of hydatidiform mole decidua and trophoblast extracts.
abstract: OBJECTIVE
  The immunologic privilege afforded the fetus relies upon immunoregulation within the maternal-fetal interface. Trophoblast and decidua-derived immunoregulatory factors enforce this privilege by locally suppressing maternal responses to trophoblast antigens. The relative contribution of trophoblast or decidua immunosuppressive factors to pregnancy immunotolerance are not well characterized. The purpose of this study was to compare the suppressive effects of hydatidiform mole trophoblast and decidua extracts on interleukin-2-dependent proliferation.
  
  
  METHODS
  Tissue extracts were prepared from hydatidiform mole trophoblast and decidua following uterine evacuation. Samples were submitted to interleukin-2-dependent and -independent cell proliferation assays.
  
  
  RESULTS
  Hydatidiform mole trophoblast extract significantly (P < 0.05) suppressed interleukin-2-dependent proliferation but did not affect interleukin-2-dependent cell proliferation. In contrast, molar decidua extract suppressed both cell lines.
  
  
  CONCLUSIONS
  Human hydatidiform mole trophoblast contains factor(s) that specifically abrogate interleukin-2-dependent clonal expansion of murine cytotoxic T-cells. In contrast, extracts of molar decidua suppressed both interleukin-2-dependent and -independent responses. This indicates that the trophoblast antagonizes critical interleukin-2-mediated immunologic responses, but that the decidua uses nonspecific antiproliferative mechanisms for immunoregulation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson 39216-4505, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Bennett
authors.forename: M N
authors.initials: MN
authors.lastname: Brackin
authors.forename: C A
authors.initials: CA
authors.lastname: Long
authors.forename: B D
authors.initials: BD
authors.lastname: Cowan
mesh_terms: D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000032:analysis*; D007376:Interleukin-2 / Q000037:antagonists & inhibitors; D011247:Pregnancy; D014327:Trophoblasts / Q000276:immunology*; D014594:Uterine Neoplasms / Q000276:immunology*
chemical_list: D007166:Immunosuppressive Agents; D007376:Interleukin-2