title: Behavioral effects of 3 alpha-androstanediol. I: Modulation of sexual receptivity and promotion of GABA-stimulated chloride flux.
abstract: Pregnane neurosteroids may initiate sexual receptivity not only via actions at intracellular receptors, but by affecting gamma-aminobutyric acid (GABA) receptor complexes (GBRs). To investigate whether GBR-mediated actions of an androgenic neurosteroid 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-androstanediol; 3 alpha-Diol) may influence the expression of sexual behavior, ovariectomized (ovx) rats received daily injections of 3 alpha-Diol (0.6, 3.0, 6.0 and 7.5 mg/kg) or vehicle (10% (v/v) ethanol in propylene glycol) at 10.00 h, and s.c. injections of estradiol-17 beta (E2: 1 microgram/0.2 ml in 10% ethanol) at 13.00 h and 19.00 h. Progesterone (P: 0.5, 1.0, 2.0 and 4.0 mg/kg) or sesame-oil vehicle was given at 12.30 h on the day following two days of 3 alpha-Diol and E2 treatment. In Expt. 1, levels of sexual receptivity were measured at 18.00-19.00 h, 56-57 h after the first injection of 3 alpha-Diol and 4 h after P or vehicle injection. 3 alpha-Androstanediol (6.0 mg/kg) attenuated sexual behavior (lordosis quotient, lordosis rating) and facilitated aggressive/rejection behaviors following 0.0, 1.0, 2.0 and 4.0 mg/kg P. The highest dosage of 3 alpha-Diol (7.5 mg/kg) facilitated sexual behavior and inhibited aggression behaviors following 0.0, 1.0, 2.0 and 4.0 mg/kg P. In Expt. 2, GABA-stimulated chloride flux was greater in cortical synaptoneurosomes of animals that received hormone treatments associated with inhibited receptivity (E2 + P + 3 alpha-Diol 3.0 mg/kg) than following treatments that facilitated receptivity (E2 + P and E2 + P + 3 alpha-Diol 7.5 mg/kg) or unreceptive ovx animals. In Expt. 3, circulating concentrations of 3 alpha-Diol resulting from the 0.0, 3.0 and 7.5 mg/kg s.c. doses administered to E2- and P-primed animals was measured by radioimmunoassay. Circulating levels of 3 alpha-Diol at the completion of behavioral testing were comparable to those previously ascertained across the estrous cycle. These data indicate that 3 alpha-Diol influences the expression of E2 and P-induced receptivity, and suggest that 3 alpha-Diol, like other neurosteroids, may exert its effects on sexual behavior by actions at GBRs.
authors.affiliation: Department of Biology, Boston University, MA 02215, USA. cafry@conncoll.edu
authors.forename: C A
authors.initials: CA
authors.lastname: Frye
authors.forename: K R
authors.initials: KR
authors.lastname: Van Keuren
authors.forename: M S
authors.initials: MS
authors.lastname: Erskine
mesh_terms: D000374:Aggression / Q000187:drug effects; D015113:Androstane-3,17-diol / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus / Q000187:drug effects; D005260:Female; D008297:Male; D011187:Posture / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D018118:Chloride Channels; D015113:Androstane-3,17-diol; D011374:Progesterone; D004958:Estradiol; D005680:gamma-Aminobutyric Acid