Characterization of an inhibitor-resistant enzyme IRT-2 derived from TEM-2 beta-lactamase produced by Proteus mirabilis strains.
Ten clinical isolates of Proteus mirabilis were found to produce an inhibitor-resistant TEM beta-lactamase (IRT) in association with a TEM-1 enzyme. The IRT enzyme was derived from TEM-2. The blaIRT gene differs from blaTEM-2 gene by one point mutation which leads to the amino-acid substitution Arg-->Ser at position 244, as observed for the original IRT-2 enzyme derived from TEM-1 reported in Escherichia coli. This is the first report of an IRT beta-lactamase derived from TEM-2.
Bret
L
L
Laboratoire de Bactériologie, Faculté de Médecine, Université d'Auvergne, Clermont-Ferrand, France.
Chanal
C
C
Sirot
D
D
Labia
R
R
Sirot
J
J
D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D010641:Phenotype; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology*; D001618:beta-Lactamases / Q000302:isolation & purification* / Q000378:metabolism*
D001618:beta-Lactamases; C056342:beta-lactamase TEM-2