Immunohistochemical localization and quantitative analysis of cellular glutathione peroxidase in foetal and neonatal rat tissues: fluorescence microscopy image analysis.
To quantitate the developmental changes in selenium-dependent cellular glutathione peroxidase during the perinatal period, tissue sections from foetal (day 12 to day 22) and neonatal (day 6) rats were stained immunohistochemically using specific polyclonal antiserum. The intensity of the staining was quantified by fluorescence microscopy image analysis. There was a general trend of enriched glutathione peroxidase in the epithelial linings and metabolically active sites. Significant fluorescence was detected in cardiomyocytes, hepatocytes, renal tubular epithelium, bronchiolar epithelium and intestinal epithelium at day 15. The intensity increased in a stepwise manner thereafter. The overall increase in the intensity of staining in the heart, liver, kidneys, lungs and intestine was 1.5-, 2.3-, 1.6-, 1.7- and 3.0-fold, respectively. The phase of most rapid increase occurred during the foetal period in the liver, intestine and heart. In the kidneys and lungs, glutathione peroxidase increased significantly during foetal life, and to a similar extent postnatally. These results suggest that the intracellular H2O2-scavenging system develops during the foetal period as an essential mechanism for living under atmospheric oxygen conditions. The late development observed in the kidneys and lungs is consistent with the relative biological immaturity of these organs in full-term neonates.
Asayama
K
K
Department of Pediatrics, Yamanashi Medical University, Japan.
Dobashi
K
K
Kawada
Y
Y
Nakane
T
T
Kawaoi
A
A
Nakazawa
S
S
D000818:Animals; D000831:Animals, Newborn; D001980:Bronchi / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D004847:Epithelial Cells; D004848:Epithelium / Q000196:embryology / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006321:Heart / Q000196:embryology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007422:Intestines / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D007684:Kidney Tubules / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D008297:Male; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase