title: Effect of bovine herpesvirus-1 on expression of interleukin-2 receptors and effect of interleukin-12 on lymphocyte proliferation.
abstract: Expression of the interleukin-2 receptor alpha chain (IL-2R alpha) by peripheral blood mononuclear cells (PBMC) from Holstein calves, both experimentally-infected with bovine herpesvirus-l (BHV-l) and controls, was measured by flow cytometry. Expression of IL-2R alpha was 35 percent and 23 percent higher in infected calves than controls, on days 2 and 3 postinfection (PI), respectively. Concurrent with this increase in IL-2R alpha expression, a significant decrease (P < 0.001) was observed in the PHA-induced proliferative responses of PBMC from infected compared with control calves. In vitro treatment with recombinant human (rhu) IL-12 enhanced PHA-induced proliferative responses of PBMC from both infected and control calves. This rhuIL-12 enhancement of mitogen-induced proliferative responses was significant (P < 0.001) in infected calves on day 2 PI and was sufficient to abrogate the decrease observed due to BHV-1 infection. Since the expression of the beta and gamma chains of IL-2R was not measured it is difficult to speculate as to the status of high affinity receptor expression during BHV-1 infection. However results of the present study suggest that the decrease in proliferative responses observed during infection may not be due to a decrease in IL-2R alpha expression but may possibly be due to a selective down-regulation of signal transduction through IL-2R and/or by modulation of the expression of other cytokines involved in lymphocyte activation and proliferation.
authors.affiliation: Department of Microbiology and Pathology, Tuskegee University, AL 36088, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Lan
authors.forename: M A
authors.initials: MA
authors.lastname: Chambers
authors.forename: J A
authors.initials: JA
authors.lastname: Ferguson
authors.forename: K K
authors.initials: KK
authors.lastname: Srivastava
authors.forename: P G
authors.initials: PG
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases; D002460:Cell Line; D002478:Cells, Cultured; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000821:virology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values
chemical_list: D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D018664:Interleukin-12