title: Glycerol is not an inhibitor of mitochondrial citrate oxidation by Aspergillus niger.
abstract: We have re-assessed the hypothesis that an accumulation of intracellular glycerol triggers the accumulation of citric acid by Aspergillus niger by inhibiting the activity of the mitochondrial NADP-dependent isocitrate dehydrogenase isoenzyme. To this end, we have incubated mycelia of A. niger with 0.5 M glycerol, which resulted in a maximal intracellular glycerol pool level of 0.92 M, comparable to that determined during the early phase of citrate accumulation. This addition affected neither the uptake of [1,5-14C] citrate from the medium nor the rate of the subsequent release of 14CO2 by the mycelia, indicating no effect on citrate oxidation. Mitochondria isolated from mycelia previously loaded with 0.5 M glycerol contained 8% of the total mycelial glycerol. They released 14CO2 from exogenously added [1,5-14C]citrate at the same rate as mitochondria isolated from mycelia not loaded with 0.5 M glycerol. The addition of glycerol had no effect on the activity of the purified NADP-specific isocitrate dehydrogenase, but appeared to inhibit the activity in crude cell-free extracts of A. niger. We conclude that the intracellular accumulation of glycerol does not affect the rate of mitochondrial citrate oxidation and is therefore, in contrast to previous claims, not a trigger of citrate accumulation.
authors.affiliation: Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU, Wien, Austria.
authors.forename: I
authors.initials: I
authors.lastname: Arisan-Atac
authors.forename: C P
authors.initials: CP
authors.lastname: Kubicek
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism; D002458:Cell Fractionation; D019343:Citric Acid / Q000378:metabolism*; D005990:Glycerol / Q000032:analysis / Q000494:pharmacology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D002245:Carbon Dioxide; D019343:Citric Acid; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D005990:Glycerol