Transformation of Pleurotus sapidus protoplasts by electroporation.
The total DNA of Pleurotus ostreatus was successfully transferred into the protoplasts of monokaryotic mycelia of Pleurotus sapidus by electroporation. The transformants were selected by their clamp connection of dikaryocyte, and the transformation frequency and transformation rate were 8.2 x 10(-5) and 3.6%, respectively. Analyzing the esterase isozyme, it was found that the transformants showed some isozyme bands of P. ostreatus and P. sapidus, and new non-parental bands. This proved that the recombination occurred between the DNAs of P. sapidus and P. ostreatus. The fruit bodies of the transformants showed morphological changes.
Yan
K
K
Department of Biology, Hebei University, Baoding, China.
Zhu
B
B
Cheng
Y
Y
Li
Q
Q
Zhao
H
H
D018274:Electroporation; D004950:Esterases / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D009024:Morphogenesis; D011124:Polyporaceae / Q000235:genetics* / Q000254:growth & development; D011523:Protoplasts; D012641:Selection, Genetic; D013045:Species Specificity; D014170:Transformation, Genetic
D007527:Isoenzymes; D004950:Esterases