title: Free alanine, aspartic acid, or glutamic acid reduce the glycation of human lens proteins.
abstract: The amino acids lysine and glycine are reported to react with glucose at physiological pH and temperature and undergo non-enzymic glycation. Three other amino acids present in relatively larger amounts in the lens i.e. alanine, aspartic acid and glutamic acid were also found to undergo non-enzymic glycation as found by incorporation of uniformly labelled (U-[14C]) glucose into the amino acids. The glucose incorporation was 1.6 to 2.5% for alanine, 35 to 50% for aspartic acid and 2.3 to 3.3% for glutamic acid. Each amino acid of varying concentrations lowered the extent of in vitro glycation of lens proteins significantly in glucose-treated homogenates of normal lens from humans. The decrease in glycation for alanine was between 32 and 69%, that for aspartate was between 18 and 74%, and for glutamate was between 52 to 74%. Decreased glycation was greater for higher concentrations of glucose. Scavenging of intracellular glucose and decreasing the extent of glycation of lens proteins could be the mechanism of action by which the amino acids alanine, aspartic acid and glutamic acid could exercise a beneficial effect on cataract and diabetic retinopathy.
authors.affiliation: Department of Biochemistry, Vision Research Foundation, Madras, India.
authors.forename: S
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: K N
authors.initials: KN
authors.lastname: Sulochana
authors.forename: R
authors.initials: R
authors.lastname: Punitham
authors.forename: K
authors.initials: K
authors.lastname: Arunagiri
mesh_terms: D000409:Alanine / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans
chemical_list: D003459:Crystallins; D001224:Aspartic Acid; D018698:Glutamic Acid; D000409:Alanine