title: A rapid method for purification of monoclonal human IgM from mass culture.
abstract: We describe in this paper the rapid and cheap purification of human immunoglobulin M from hybridoma supernatant of mass culture. The method consists of two steps: 1. concentration of supernatant by ultrafiltration and 2. dialysis against distilled water, pH 6.4. To produce the supernatant, the hybridomas are grown in RPMI media with fetal calf serum (10% FCS) in 250 ml flasks under normal tissue culture conditions. More than 14 mg IgM can be nearly selectively purified within 6 h from 5 l of antibody containing hybridoma supernatant. Most of the IgM molecules stay in a penta- and monomeric form and are positive in physiological and immunohistochemical studies.
authors.affiliation: Institut für Pathologie, Universität Würzburg, Germany.
authors.forename: H P
authors.initials: HP
authors.lastname: Vollmers
authors.forename: E
authors.initials: E
authors.lastname: Wozniak
authors.forename: E
authors.initials: E
authors.lastname: Stepien-Bötsch
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.forename: H K
authors.initials: HK
authors.lastname: Müller-Hermelink
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D003956:Dialysis / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000302:isolation & purification*; D009101:Multiple Myeloma / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology; D014867:Water
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D014867:Water