title: Prolonged final extension time increases cloning efficiency of PCR products.
authors.affiliation: University of Florida, Gainesville, USA.
authors.forename: Q B
authors.initials: QB
authors.lastname: Li
authors.forename: C L
authors.initials: CL
authors.lastname: Guy
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007763:Lac Operon; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D013997:Time Factors
chemical_list: D018744:DNA, Plant; D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D012273:Ribulose-Bisphosphate Carboxylase