Glycine activation of human homomeric alpha 1 glycine receptors is sensitive to pressure in the range of the high pressure nervous syndrome.
The effect of hyperbaric pressure on the inhibitory glycine receptor has been investigated in voltage-clamped Xenopus oocytes microinjected with cRNA encoding the human alpha-1 glycine receptor subunit. Heterologous expression of the human alpha-1 subunit generated functional glycine-gated channels with properties typical of native receptors. Glycine elicited a concentration-dependent inward current which reversed polarity at -25 mV and was antagonised by nanomolar concentrations of strychnine. Concentration-response curves established for the homomeric alpha-1 glycine receptor at 5, 10 and 15 MPa were progressively shifted to the right with respect to the concentration response curve established at atmospheric pressure (0.1 MPa). Pressure had no effect on the maximal response. The EC(50) values at 0.1, 5, 10 and 15 MPa were 190 mu M, 222 mu M, 338 mu M and 482 mu M, respectively. The results demonstrate that a receptor comprised solely of the human alpha-subunit is sensitive to pressure in the range that affects divers and at which the native rat spinal cord receptor is affected. This finding is discussed in the context of the postulated binding sites for glycine and the implications for the design of drugs to protect divers from the effects of pressure.
Roberts
R J
RJ
The Hyperbaric Group, Welsh School of Pharmacy, University of Wales Cardiff, UK.
Shelton
C J
CJ
Daniels
S
S
Smith
E B
EB
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005998:Glycine / Q000494:pharmacology*; D006610:High Pressure Neurological Syndrome / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D018009:Receptors, Glycine / Q000819:agonists*; D012680:Sensitivity and Specificity; D014982:Xenopus laevis
D018009:Receptors, Glycine; D005998:Glycine