title: Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: production, characterization, and immunodiagnostic application.
abstract: A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the "gold standard," the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
authors.forename: F
authors.initials: F
authors.lastname: Qadri
authors.forename: M G
authors.initials: MG
authors.lastname: Mohi
authors.forename: A
authors.initials: A
authors.lastname: Chowdhury
authors.forename: K
authors.initials: K
authors.lastname: Alam
authors.forename: T
authors.initials: T
authors.lastname: Azim
authors.forename: C
authors.initials: C
authors.lastname: Sears
authors.forename: R B
authors.initials: RB
authors.lastname: Sack
authors.forename: M J
authors.initials: MJ
authors.lastname: Albert
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D001441:Bacteroides fragilis / Q000276:immunology*; D004768:Enterotoxins / Q000037:antagonists & inhibitors / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004768:Enterotoxins