title: Porphyromonas gingivalis surface components induce interleukin-1 release and tyrosine phosphorylation in macrophages.
abstract: The aim of the present study was to characterize the responses of macrophages to surface antigens of Porphyromonas gingivalis. Native fimbriae, full-length recombinant fimbrillin, and a lectin-like 12-kDa antigen all stimulated BALB/c peritoneal macrophages to secrete interleukin (IL)-1 beta. The antigens induced similar patterns of tyrosine phosphorylation; proteins in approximately 35-46 kDa range of undetermined identities were phosphorylated in the macrophages. The abilities of the surface antigens to induce IL-1 beta were markedly attenuated by tyrosine kinase inhibitors. This inhibition correlated with inhibition of the induced phosphorylation of specific macrophage proteins at tyrosine. The data suggest that tyrosine kinase(s) plays an important role in the regulatory intracellular signaling mechanisms by which P. gingivalis surface antigens can mediate certain responses in macrophages.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214-3092, USA.
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: H T
authors.initials: HT
authors.lastname: Sojar
authors.forename: R J
authors.initials: RJ
authors.lastname: Genco
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000633:toxicity*; D001426:Bacterial Proteins / Q000633:toxicity; D016227:Benzoquinones; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D036781:Fimbriae Proteins; D019833:Genistein; D007375:Interleukin-1 / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010766:Phosphorylation / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D014443:Tyrosine / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D016227:Benzoquinones; D004791:Enzyme Inhibitors; D007375:Interleukin-1; D007529:Isoflavones; D047029:Lactams, Macrocyclic; D011809:Quinones; C063055:fimbrillin; D036781:Fimbriae Proteins; D017828:Rifabutin; D014443:Tyrosine; C020754:herbimycin; D019833:Genistein; D011505:Protein-Tyrosine Kinases