Myocardial reactive hyperaemia in perfused guinea pig heart.
Reactive hyperaemia (RH) was induced by occlusion of coronary flow (1-60 s) in perfused guinea pig heart. It was inhibited by 100-60%, by NG-nitro-L-arginine (100 microM) and to a lesser extent (up to 35%) by 8-phenyltheophylline (10 microM). Indomethacin (5 microM), did not affect RH. During RH the heart generated prostacyclin, nitric oxide and adenosine as indicated by the appearance of 6-keto-PGF1 alpha, cyclic GMP, inosine, hypoxanthine, xanthine and urate in the perfusate. Only nitric oxide (NO) and adenosine but not prostacyclin were responsible for RH. NO mediated RH which followed short-term (1-10 s) coronary occlusion whereas a combined effort of NO and adenosine was required to maintain RH that followed longer (20-60 s) periods of interruption of coronary inflow. Prostacyclin did not participate in RH at any circumstances.
Gryglewski
R J
RJ
Department of Pharmacology, Medical College of Jagiellonian University, Kraków, Poland.
Chłopicki
S
S
Niezabitowski
P
P
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D006152:Cyclic GMP / Q000378:metabolism; D004562:Electrocardiography / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D011464:Epoprostenol / Q000378:metabolism / Q000502:physiology; D006168:Guinea Pigs; D006940:Hyperemia / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D058915:Purinergic P1 Receptor Antagonists; D011687:Purines / Q000378:metabolism; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D058915:Purinergic P1 Receptor Antagonists; D011687:Purines; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D015121:6-Ketoprostaglandin F1 alpha; D013806:Theophylline; D011464:Epoprostenol; C028322:8-phenyltheophylline; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D000241:Adenosine