title: Molecular cloning, expression and chromosomal localization of a human gene encoding a 33 kDa putative metallopeptidase (PRSM1).
abstract: The zincins are a superfamily of structurally-related Zn(2+)-binding metallopeptidases which play a major role in a wide range of biological processes including pattern formation, growth factor activation and extracellular matrix synthesis and degradation. In this paper we report the identification and complete primary structure of a novel 33 kDa protein which contains the zinc-binding HEXXH motif found in the zincin superfamily. We have named this novel protein PRSM1 (PRoteaSe, Metallo, number 1). The gene was identified by the immunoscreening of a human placental cDNA library using polyclonal antibodies raised to the 70 kDa human matrix metalloendopeptidase, type III procollagen N-proteinase [Halila, R. and Peltonen, L. (1986) Purification of human procollagen type III N-proteinase from placenta and preparation of antiserum. Biochem. J. 239, 47-52]. The protein is found in placenta and cultured osteosarcoma cells. PRSM1 could share sequence homology with the type III procollagen N-proteinase. The prsm1 gene is represented once in the human genome and is localized on chromosome 16 (q24.3).
authors.affiliation: Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, School of Biological Sciences, UK.
authors.forename: I C
authors.initials: IC
authors.lastname: Scott
authors.forename: R
authors.initials: R
authors.lastname: Halila
authors.forename: J M
authors.initials: JM
authors.lastname: Jenkins
authors.forename: S
authors.initials: S
authors.lastname: Mehan
authors.forename: S
authors.initials: S
authors.lastname: Apostolou
authors.forename: R
authors.initials: R
authors.lastname: Winqvist
authors.forename: D F
authors.initials: DF
authors.lastname: Callen
authors.forename: D J
authors.initials: DJ
authors.lastname: Prockop
authors.forename: L
authors.initials: L
authors.lastname: Peltonen
authors.forename: K E
authors.initials: KE
authors.lastname: Kadler
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D008666:Metalloendopeptidases