title: GeneFisher--software support for the detection of postulated genes.
abstract: When a family of genes from closely related organisms is known, there is a certain change to extract the corresponding gene from the genome of another related organism. This can be done by polymerase chain reaction, provided that a pair of suitable primers can be designed. In contrast to primer design for a single, known target sequence, systematic primer design for an unknown target given a group of homologues can by no means be done manually. GeneFisher is a software tool which automates this task, and takes special care to make the impact of the manifold design parameters transparent to the user.
authors.affiliation: robert@techfak.uni-bielefeld.de
authors.forename: R
authors.initials: R
authors.lastname: Giegerich
authors.forename: F
authors.initials: F
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Schleiermacher
mesh_terms: D000465:Algorithms; D017931:DNA Primers / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers