title: Basal Fos immunoreactivity in myenteric neurons of guinea pig.
abstract: The enteric nervous system (ENS) is capable of mediating intestinal reflexes in the absence of connections to the CNS. Nuclear immunoreactivity for Fos, a protein encoded by the c-fos proto-oncogene, has previously been used to visualize activated cells. We have attempted to use a commercial antibody to Fos to investigate the proportion of activated neurons in the myenteric ganglia of guinea pigs. Neuronal nuclei which demonstrated Fos Immunoreactivity (F-IR) were counted in preparations fixed immediately after removal from the animals. Demonstration of all myenteric neurons in adjacent segments by staining with cuprolinic blue revealed that neurons with F-IR comprised a subset (65-75%) of all neurons. These results have led us to conclude that ENS has a basal activity for intestinal functions and this could be demonstrated easily by Fos immunoreactivity.
authors.affiliation: Hacettepe University, Faculty of Medicine, Dept. of Histology & Embryology, Ankara, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Karaosmanoğlu
authors.forename: M K
authors.initials: MK
authors.lastname: Irmak
authors.forename: A
authors.initials: A
authors.lastname: Seftalioğlu
mesh_terms: D000818:Animals; D004396:Coloring Agents; D006168:Guinea Pigs; D007211:Indoles; D007422:Intestines / Q000294:innervation; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology*; D009474:Neurons / Q000737:chemistry*; D009942:Organometallic Compounds; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000276:immunology*; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents; D007211:Indoles; D009942:Organometallic Compounds; D016760:Proto-Oncogene Proteins c-fos; C015445:copper phthalocyanine