Diazepam-insensitive GABAA receptors in rat cerebellum and thalamus.
Three major populations of GABAA receptor binding sites are present in cerebellar membranes: diazepam-sensitive [3H]Ro15-4513 binding sites, diazepam-insensitive [3H]Ro15-4513 binding sites and high-affinity [3H]muscimol binding sites. All three populations contain a beta subunit as shown by immunoprecipitation with antibodies that recognize all beta subunits. The beta 3 subtype of beta subunit is contained in all three populations, but only a similar low fraction (< 20%) in each. Thus, the majority contain beta subunits other than beta 3 (beta 2 and beta 1) and beta 3 subunits are not selectively associated with nor lacking in any of the three binding populations. Antibodies to the gamma 2 subunit precipitated similar fractions of [3H]Ro15-4513, [3H]flunitrazepam and [3H]muscimol binding sites, showing that gamma 2 subunits are present in high-affinity muscimol binding isoforms, as well as a significant fraction of the diazepam-insensitive [3H]Ro15-4513 binding sites. Under conditions that identify the 56 kDa alpha 6 subunit on SDS-PAGE as the diazepam insensitive site of [3H]Ro15-4513 binding in cerebellum, no polypeptide showing diazepam-insensitive binding of [3H]Ro15-4513 could be photoaffinity-labeled in rat thalamus. These results suggest that alpha 4 subunits in the thalamus participate primarily in subunit combinations which bind muscimol but not any benzodiazepine site ligands.
Huh
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KH
Department of Molecular and Medical Pharmacology, University of California at Los Angeles 90095, USA.
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Olsen
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D000345:Affinity Labels; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001665:Binding Sites; D002531:Cerebellum / Q000378:metabolism*; D003975:Diazepam / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013788:Thalamus / Q000378:metabolism*; D014316:Tritium
D000345:Affinity Labels; D000906:Antibodies; D011963:Receptors, GABA-A; D014316:Tritium; D003975:Diazepam