Thermococcus fumicolans sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent in the north Fiji Basin.
An extremely thermophilic archaeon, strain ST557T (T = type strain), was isolated from a deep-sea hydrothermal vent in the North Fiji Basin. This strain is a strictly anaerbic coccus whose cells are about 0.8 to 2 microns in diameter. The optimum temperature, pH and sea salt concentration for growth are 85 degrees C, 8.5, and 20 to 40 g/liter, respectively. Strain ST557T grows preferentially in the presence of elemental sulfur on proteinaceous substrates and on a mixture of 20 amino acids. It grows slowly on pyruvate and maltose. Growth is inhibited by rifampin. The DNA G + C content is 54 to 55 mol%. Sequencing of the 16S rRNA gene revealed that strain ST557T belongs to the genus Thermococcus. We propose that this organism should be placed in a new species, Thermococcus fumicolans.
Godfroy
A
A
Département Environnement Profond IFREMER, Centre de Brest, Plouzané, France.
Meunier
J R
JR
Guezennec
J
J
Lesongeur
F
F
Raguénès
G
G
Rimbault
A
A
Barbier
G
G
D001105:Archaea / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D013696:Temperature; D014871:Water Microbiology
D004269:DNA, Bacterial; D008055:Lipids; D012336:RNA, Ribosomal, 16S