Activation of Na+/H+ exchanger is not involved in the PDGF-BB-induced DNA-synthesis in vascular smooth muscle cells.
HOE 694 [(3-methylsulphonyl-4-piperidinobenzoyl)guanidine methanesulphonate] was characterized as a new, potent, non-amiloride inhibitor of the Na+/H+ exchanger. In order to elucidate whether the Na+/H+ exchanger isoform 1 (NHE-1) is involved in the PDGF-BB-induced DNA-synthesis in vascular smooth muscle cells, we investigated the effect of HOE 694 and EIPA on the PDGF-BB-induced DNA synthesis. EIPA (10 microM) inhibited the PDGF-BB-induced stimulation of the Na+/H+ exchanger and the PDGF-BB-induced DNA synthesis in a concentration-dependent manner. HOE 694 (1-50 microM) dose-dependently inhibited the PDGF-BB-induced stimulation of the Na+/H+ exchanger but had no significant effects on DNA-synthesis. Our findings suggest that activation of the Na+/H+ exchanger is not essential for PDGF-BB-induced vascular smooth muscle cell growth.
Sachinidis
A
A
Medizinische Universitäts-Poliklinik, Bonn, Germany.
Seul
C
C
Seewald
S
S
Ko
Y
Y
Vetter
H
H
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic; D000077214:Becaplermin; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006146:Guanidines / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D011921:Rats, Inbred WKY; D011994:Recombinant Proteins / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D013450:Sulfones / Q000494:pharmacology*
D006146:Guanidines; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D017923:Sodium-Hydrogen Exchangers; D013450:Sulfones; C069139:growth factor-activatable Na-H exchanger NHE-1; C074107:3-methylsulfonyl-4-piperidinobenzoyl guanidine; D000077214:Becaplermin; D000584:Amiloride; D004247:DNA; D012964:Sodium; C039614:ethylisopropylamiloride