Different sensitivity to streptolysin-O of cells in macrophage lineage.
We have surveyed the sensitivity of cells in macrophage lineage to Streptolysin-O (SLO). SLO had cytotoxic activity on immature myeloid cell lines such as M1 and WEHI-3BD+. SLO was toxic to the cells after a 2-hr incubation. However, mature macrophage cell lines such as A640-BB-2, J774, and P388D1 were not sensitive to the same dose of SLO. After M1 cells were treated with leukemia inhibitory factor (LIF), a differentiation-inducer to macrophage, these cells became insensitive to SLO in one day. This cytotoxic action of SLO was inhibited by pretreatment with anti-Streptolysin-O antibody or cholesterol. These results indicate that SLO has different effects on macrophage lineage.
Tanigawa
T
T
Department of Bacteriology, Tottori University, Yonago, Japan.
Suzuki
J
J
Ueta
T
T
Katsumoto
T
T
Tanaka
Y
Y
D019138:Antibodies, Blocking / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D002784:Cholesterol / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006131:Growth Inhibitors / Q000494:pharmacology; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D013301:Streptolysins / Q000633:toxicity*
D019138:Antibodies, Blocking; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D013301:Streptolysins; D002784:Cholesterol