Platelet function and platelet-leukocyte adhesion in symptomatic coronary heart disease. Effects of intravenous magnesium.
Spontaneous P-selectin surface expression on platelets and platelet-leukocyte adhesion was increased in patients with symptomatic coronary heart disease (n = 12) compared to normal controls (n = 10) (p < 0.05). ADP-induced P-selectin expression and platelet-leukocyte adhesion was also enhanced in the patient group (p < 0.05). Administration of intravenous Mg2+ significantly reduced both platelet surface expression of P-selectin and platelet-leukocyte adhesion ex vivo (p < 0.02). The effect of extracellular Mg2+ was evaluated in in vitro experiments. Both in whole blood and in isolated neutrophil suspension Mg2+ inhibited platelet adhesion to neutrophils dose dependently with half maximal effects at 4 mM. Moreover, Mg2+ inhibited adhesion of isolated platelet membranes to neutrophils. We conclude that platelet function is altered in symptomatic coronary heart disease and can be modulated by administration of intravenous Mg2+.
Gawaz
M
M
1. Medizinische Klinik, Technischen Universität München, Germany.
Reininger
A
A
Neumann
F J
FJ
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000787:Angina Pectoris / Q000097:blood; D001241:Aspirin / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D003327:Coronary Disease / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D007962:Leukocytes / Q000502:physiology*; D008274:Magnesium / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013927:Thrombosis / Q000517:prevention & control
D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D000244:Adenosine Diphosphate; D008274:Magnesium; D001241:Aspirin