title: Responses of oral epithelial cells to dental resin components.
abstract: The light-polymerized resins used in dentistry and their various constituents have been shown to produce significant levels of cytotoxicity, depending upon the material and the cell type exposed to it. These responses include altered cell growth and macromolecule synthesis. The current study examined the effects of several resin components on growth and lipid metabolism of oral epithelial cells. Resin discs were fabricated from triethyleneglycol dimethacrylate (TEGDMA) as received from the manufacturer and after removal of the stabilizer methyl ether hydroquinone (MEHQ). Some discs also contained the initiators benzoyl peroxide (BPO) and camphoroquinone (CQ), and/or an activator, dimethylaminoethyl methacrylate (DMAEMA). After polymerization, the ability of components to elute from the discs and alter cell growth and lipid synthesis were assayed by a colorimetric method and thin layer chromatography respectively. Purified TEGDMA had little effect on the cells' growth or lipid metabolism, while TEGDMA containing MEHQ did inhibit growth as well as total polar lipid synthesis. Eluates from discs containing DMAEMA inhibited cell growth as well as decreasing polar lipid formation. However, this same material produced increased synthesis of diglycerides and cholesterol esters. Eluates from BPO-containing discs, as well as those with CQ, with or without DMAEMA resulted in increased levels of diglycerides. These results demonstrate that even after polymerization, components used in dental resins may elute into the immediate environment and alter various cell metabolic processes.
authors.affiliation: Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1260, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Lefebvre
authors.forename: G S
authors.initials: GS
authors.lastname: Schuster
authors.forename: F A
authors.initials: FA
authors.lastname: Rueggeberg
authors.forename: K
authors.initials: K
authors.lastname: Tamareselvy
authors.forename: K L
authors.initials: KL
authors.lastname: Knoernschild
mesh_terms: D000818:Animals; D001585:Benzoyl Peroxide / Q000302:isolation & purification; D001672:Biocompatible Materials / Q000009:adverse effects / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003188:Composite Resins / Q000009:adverse effects*; D006224:Cricetinae; D003764:Dental Materials / Q000009:adverse effects* / Q000627:therapeutic use; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D006873:Hydroquinones / Q000302:isolation & purification; D008055:Lipids / Q000096:biosynthesis; D008689:Methacrylates / Q000737:chemistry / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000009:adverse effects*; D011109:Polymethacrylic Acids / Q000009:adverse effects*
chemical_list: D001672:Biocompatible Materials; D003188:Composite Resins; D003764:Dental Materials; D006873:Hydroquinones; D008055:Lipids; D008689:Methacrylates; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D001585:Benzoyl Peroxide; C031927:hydroquinone