Molecular cloning of the novel human G protein-coupled receptor (GPCR) gene mapped on chromosome 9.
A novel human GPCR gene was cloned by PCR with degenerate primers designed from cannabinoid type 1 receptor (CB1) sequences and a full-length clone was isolated by screening of a human genomic DNA library. This gene, termed EDG-3, is highly homologous (51.9 % overall and 69.2 % in seven transmembrane regions) to human EDG-1, 47.9 % to rat AGR16 but only 28.0 % to human CB1 receptor. The Northern hybridization analysis showed that a 2.8 kb transcript of EDG-3 is abundantly expressed in the heart followed by placenta, kidney and liver. The EDG-3 gene was mapped on the human chromosome 9q22.1-q22.2 by fluorescence in situ hybridization analysis. Although the ligand and physiological role of this receptor is unknown, this gene may be a new member of the EDG family.
Yamaguchi
F
F
Department of Physiology, Faculty of Medicine, Kagawa Medical University, Japan.
Tokuda
M
M
Hatase
O
O
Brenner
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D005260:Female; D015698:Genomic Library; D006801:Humans; D020672:I-kappa B Proteins; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D051381:Rats; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000235:genetics; D049312:Receptors, Lysophospholipid; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D014158:Transcription, Genetic
D017931:DNA Primers; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; C000606206:Nfkbia protein, rat; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D049312:Receptors, Lysophospholipid; D011994:Recombinant Proteins; D000072000:NF-KappaB Inhibitor alpha