title: Dacron stimulation of macrophage transforming growth factor-beta release.
abstract: This study evaluated the effect of Dacron on the release of macrophage transforming growth factor-beta (TGF-beta),an endothelial cell growth inhibitor. Rabbit peritoneal macrophages were grown in minimum essential medium (MEM) with 10% fetal bovine serum (FBS) in the presence or absence of Dacron (0.5 mm x 3 mm particles). Media were collected three times each week for 7 weeks. For the TGF-beta bioassay, mink lung epithelial cells (CCL64) were grown in MEM with 10% FBS. Test-conditioned media, 100 mu 1, were added (n = 4), and incubated 48 h. 3(H)-Thymidine (3(H)-TdR) uptake was determined and compared with 3(H)-TdR uptake using known pure TGF-beta standards. Media samples were additionally pre-incubated with a neutralizing anti-TGF-beta(1) antibody and the 3(H)-TdR uptake again quantitated. TGF-beta activity in the conditioned media of macrophages exposed to Dacron exceeded the control media groups in all weeks, reaching significance (P<0.05) in weeks 3, 4,5, 6 and 7. Pre-incubation of media samples with the anti-TGF-beta antibody inhibited this TGF-beta activity in all weeks with statistical significance in weeks 1, 2, 3, 5 and 7. The inhibitory effects of Dacron on endothelialization may be explained by the Dacron-induced release of TGF-beta from macrophages.
authors.affiliation: Loyola University Medical Center, Department of Surgery, Maywood, Illinois, USA.
authors.forename: H P
authors.initials: HP
authors.lastname: Greisler
authors.forename: D
authors.initials: D
authors.lastname: Petsikas
authors.forename: D J
authors.initials: DJ
authors.lastname: Cziperle
authors.forename: P M
authors.initials: PM
authors.lastname: Murchan
authors.forename: S C
authors.initials: SC
authors.lastname: Henderson
authors.forename: T M
authors.initials: TM
authors.lastname: Lam
mesh_terms: D000818:Animals; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism*; D008907:Mink; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D011093:Polyethylene Terephthalates; D016212:Transforming Growth Factor beta