title: The early response of pineal N-acetyltransferase activity, melatonin and catecholamine levels in rats irradiated with gamma rays.
abstract: Male Wistar rats adapted to an artificial light-dark regimen (12 h light: 12 h darkness) were whole-body irradiated with a dose of 14.35 Gy of gamma rays. Irradiation, sham-irradiation and decapitation 30, 60 and 120 min after the exposure were performed between 2000 h and 0100 h in the darkness. The serotonin N-acetyltransferase activity (NAT), the concentration of melatonin, dopamine, norepinephrine and epinephrine were measured in the pineal gland. The serum levels of melatonin and corticosterone were also determined. Ionizing radiation did not change the activity of the key enzyme of melatonin synthesis, NAT, but decreased the concentration of pineal melatonin. The concentration of pineal dopamine and norepinephrine decreased 30 and 120 min after exposure, while the concentration of epinephrine was elevated 30 min after irradiation, though later it was markedly decreased. The serum melatonin level was not changed, but an increase in corticosterone level was observed. In the early period after the exposure, a decrease in pineal melatonin occurred, accompanied by a decrease in pineal catecholamines. On the contrary, in the phase of developed radiation injury the signs of increased melatonin synthesis were observed on days 3 and 4 after the exposure (Kassayová et al. 1993a). The underlying mechanisms require further research.
authors.affiliation: Institute of Animal Physiology, Safárik University, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kassayová
authors.forename: E
authors.initials: E
authors.lastname: Ahlersová
authors.forename: B
authors.initials: B
authors.lastname: Pástorová
authors.forename: I
authors.initials: I
authors.lastname: Ahlers
mesh_terms: D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005720:Gamma Rays; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D010870:Pineal Gland / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001191:Arylamine N-Acetyltransferase; D008550:Melatonin; D004298:Dopamine; D009638:Norepinephrine