Spontaneous degranulation of cultured bone-marrow-derived immature mast cells from X-linked immunodeficient (Xid) mice.
IgE-mediated, antigen-dependent stimulation of immature mouse mast cells cultured in IL-3-containing media produces secretion by granule exocytosis. Similar cultured mast cells were derived from X-linked immunodeficient (Xid) mice and examined by electron microscopy. In these cultures, Xid mast cells were also immature. In contrast to cultures obtained from control mice, 10-20% of the immature mast cells of Xid origin were undergoing secretion by granule extrusion in the absence of any secretogogue. Spontaneous secretion may be related to discordered tyrosine kinase function and/or signal transduction pathways in the Xid mouse.
Dvorak
A M
AM
Department of Pathology, Beth Israel Hospital, Boston, MA 02215, USA.
Miura
T
T
Letourneau
L
L
Ishizaka
T
T
Kawakami
T
T
D000818:Animals; D001854:Bone Marrow Cells; D015550:Cell Degranulation / Q000276:immunology*; D048429:Cell Size; D002478:Cells, Cultured; D016867:Immunocompromised Host / Q000235:genetics*; D007377:Interleukin-3 / Q000494:pharmacology; D008407:Mast Cells / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000494:pharmacology
D007377:Interleukin-3; D011994:Recombinant Proteins