title: Use of the in vitro induced antibody production test (IVIAP) to elucidate inconclusive status of HIV-1 infection.
abstract: Studies have demonstrated the diagnostic usefulness of in vitro production of virus-specific antibodies by peripheral blood mononuclear cells (PMBC) from HIV-infected subjects. We evaluated the IVIAP (in vitro induced antibody production) by lympocytes from peripheral blood of 30 adult patients. Samples included 10 patients with inconclusive status of HIV-1 infection on previous serologic screening (2 blood donors, 8 with high risk behavior), 10 AIDS patients (CDC II-IV), and 10 known seronegative subjects. Positive IVIAP results were obtained on 2 of 10 inconclusive cases, earlier than seroconversion would demonstrate. The 8 remaining cases resulted in a negative outcome on IVIAP test, even in 2 seronegative homosexuals presenting Kaposi's sarcoma. Results showed 100% concordance either with a clinical/serological follow-up done after IVIAP tests or PCR performed for Kaposi's cases. Also, no false-positive or false-negative was detected on control groups. We concluded that IVIAP could identify the real status of HIV infection in a shorter time and with low cost compared to conventional serological methods currently available. The IVIAP test is easily performed so that can be considered as a routine supplemental test.
authors.affiliation: Laboratory of Virology, School Paulista of Medicine, Federal University of Sao Paulo, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Bellei
authors.forename: C
authors.initials: C
authors.lastname: Granato
authors.forename: H
authors.initials: H
authors.lastname: Tomiyama
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000328:Adult; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012698:Serologic Tests
chemical_list: D015483:HIV Antibodies