title: Pretreatment with pertussis toxin spinally, but not supraspinally, blocks the cold water swimming-induced antinociception in the mouse.
abstract: Mice exposed to cold water swimming (4 degrees C) for 3 min produced a marked antinociception. Experiments were designed to determine whether pretreatment with pertussis toxin given intrathecally (i.t.) or intracerebroventricularly (i.c.v.) attenuates cold water swimming-induced antinociception in male ICR mice. Antinociception was measured by the tail-flick test 7 min after cold water swimming. I.t. pretreatment with pertussis toxin at a dose of 0.5 microgram for 24-96 h caused a time-dependent attenuation of cold water swimming-induced antinociception. Moreover, i.t. pretreatment with pertussis toxin at doses from 0.125 to 0.5 microgram for 96 h attenuated cold water swimming-induced antinociception in a dose-dependent manner. However, i.c.v. pretreatment with pertussis toxin at doses from 0.125 to 0.5 microgram for 24-96 h did not affect the cold water swimming-induced antinociception. The present results suggest that pertussis toxin-sensitive Gi/G(o) proteins in spinal cord, but not at the supraspinal sites, are involved in cold water swimming-induced antinociception.
authors.affiliation: Department of Anesthesiology, MEB-462c, Medical College of Wisconsin, Milwaukee 53226, USA.
authors.forename: H
authors.initials: H
authors.lastname: Mizoguchi
authors.forename: M
authors.initials: M
authors.lastname: Narita
authors.forename: J P
authors.initials: JP
authors.lastname: Kampine
authors.forename: L F
authors.initials: LF
authors.lastname: Tseng
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009619:Nociceptors / Q000187:drug effects*; D037342:Pertussis Toxin; D013116:Spinal Cord / Q000187:drug effects*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin