Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15.
The cysteine synthase gene (cysK) from Flavobacterium K3-15 was cloned and sequenced. The gene exhibits 30-50% identity to known cysteine synthases on both the DNA and the amino acid levels. The pyridoxal phosphate binding site of the enzyme is part of a conserved motif comprising seven amino acids (SIKDRIA). The lys31 residue of the flavobacterial enzyme is conserved in all known cysteine synthases. The cysK gene from Flavobacterium K3-15 was heterologously expressed and the gene product identified by immunoblotting and determination of the enzyme activity.
Müller
R
R
Institut für Pharmazeutische Biologie, Universität Bonn, Germany. 100724.3020@compuserve.com
Kuttler
E
E
Lanz
C
C
Drewke
C
C
Schmidt
K
K
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003547:Cysteine Synthase / Q000235:genetics* / Q000378:metabolism; D005417:Flavobacterium / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D003547:Cysteine Synthase