Negative regulation of dorsal patterning in early embryos by overexpression of XrelA, a Xenopus homologue of NF-kappa B.
Recent results by Richardson et al. (Mech. Dev., 52 (1995) 165-177) suggest that the Xenopus Rel gene XrelA may be involved in the formation of the head and tail of the early embryo. We present evidence to suggest that wild-type XrelA also has a role in dorsoventral development. XrelA overexpression in the dorsal side of embryos reduces dorsal development and attenuates in vitro dorsal morphogenetic movements. XrelA also strongly reduces axis duplication caused by overexpression of a dominant negative mutant of Xenopus glycogen synthase kinase-3 beta. Our results indicate that XrelA may have a role in dorsoventral patterning in early embryos.
Kao
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KR
Terry Fox Cancer Research Laboratories, Division of Basic Medical Sciences, Memorial University of Newfoundland, St. John's, Canada. kkao@plato.ucs.mun.ca
Lockwood
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A
D000367:Age Factors; D000818:Animals; D019521:Body Patterning / Q000187:drug effects / Q000502:physiology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D017403:In Situ Hybridization; D016328:NF-kappa B / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D012313:RNA / Q000008:administration & dosage; D014157:Transcription Factors; D014982:Xenopus laevis
D016328:NF-kappa B; D014157:Transcription Factors; D012313:RNA; D038341:Glycogen Synthase Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3