title: The sxa2-dependent inactivation of the P-factor mating pheromone in the fission yeast Schizosaccharomyces pombe.
abstract: Haploid cells of the fission yeast Schizosaccharomyces pombe exist in one of two mating types, referred to as M and P. Conjugation occurs between cells of opposite mating type and is controlled by the reciprocal action of diffusible pheromones. Loss of function of the sxa2 gene in M cells causes hypersensitivity to the P-factor mating pheromone and a reduction in mating efficiency. Here we demonstrate the secretion of an sxa2-dependent carboxypeptidase that inactivates P-factor by removal of the C-terminal leucine residue.
authors.affiliation: School of Biochemistry, University of Birmingham, UK.
authors.forename: G
authors.initials: G
authors.lastname: Ladds
authors.forename: E M
authors.initials: EM
authors.lastname: Rasmussen
authors.forename: T
authors.initials: T
authors.lastname: Young
authors.forename: O
authors.initials: O
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Davey
mesh_terms: D002268:Carboxypeptidases / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006863:Hydrogen-Ion Concentration; D010675:Pheromones / Q000378:metabolism* / Q000494:pharmacology; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C085846:P-factor, S pombe; D010675:Pheromones; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D002268:Carboxypeptidases