Adrenomedullin: changes in circulating and cardiac tissue concentration in Dahl salt-sensitive rats on a high-salt diet.
Adrenomedullin (AM), a novel hypotensive peptide, is suggested to be involved in defense mechanisms against hypertension, however, the detail mechanisms have not been clarified. To elucidate whether AM synthesis would be altered in a salt dependent hypertension, we have investigated the AM concentration and AM messenger RNA (mRNA) level in tissues of Dahl salt-sensitive rats on either low- or high-salt intake. The AM concentration in cardiac ventricle of the high-salt group was significantly higher than that of the low-salt group. The plasma AM concentration was also significantly higher in the high-salt group than in the low-salt group. Furthermore, the plasma AM concentration correlated well with the weight of left ventricle. RNA blot analysis revealed that the AM mRNA level in cardiac ventricle of the high-salt group was higher than that of the low-salt group. These results suggest that AM participates in the pathophysiology of salt dependent hypertension and plays a role in cardiac hypertrophy.
Shimokubo
T
T
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Sakata
J
J
Kitamura
K
K
Kangawa
K
K
Matsuo
H
H
Eto
T
T
D053607:Adrenomedullin; D000818:Animals; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage*
D010455:Peptides; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary; D053607:Adrenomedullin