title: Impact of adhesion molecules of the selectin family on liver microcirculation at reperfusion following cold ischemia.
abstract: We investigated the role of adhesion molecules in the early phase of reperfusion following cold ischemia. Livers of male Lewis rats were preserved for 0 h (group A) or 24 h in University of Wisconsin (UW) solution without additives (group B) or in UW solution with anti-ICAM-1 antibody (group C) or anti-E-selectin-1, SLe(x) and SLe(a) antibodies (group D). The livers were then reperfused with diluted rat whole blood (DWB; groups A and B). DWB containing anti-ICAM-1 and LFA-1 antibodies (group C) or DWB containing anti-L-selectin, SLe(x) and SLe(a) antibodies (group D). The reperfusion was performed at 37 degrees C for 1 h at 5 cm H2O of perfusion pressure. During reperfusion, hepatic microcirculation was assessed by monitoring portal and peripheral tissue blood flow. Bile production was significantly reduced in group B livers compared with those in group A. Anti-ICAM-1 and LFA-1 antibodies failed to improve hepatic microcirculation, whereas anti-LECAM-1, SLe(x) and SLe(a) antibodies significantly improved the microcirculation. Bile production in group C and D livers was comparable to that in group B livers. Preservation for 24 h significantly increased the release of TNF-alpha from 0.207 to 43.7 pg/g per hour during reperfusion. Monoclonal antibodies to the adhesion molecules did not suppress the release of TNF-alpha in groups C and D. Histological examination demonstrated a lack of leukocyte infiltration or thrombus in hetapic microvessels. The extent of hepatocyte necrosis did not differ among groups B, C, and D. We conclude that the microcirculatory disturbance in the early phase of reperfusion occurs as a result of the tethering of leukocytes through the interaction of the selectin family and their ligands, and that the ICAM-1-LFA-1 pathway is not involved in this step. The lack of improvement in bile production with antibodies to the selectin family and their ligands strongly suggests that other mechanisms participate in the deterioration of hepatic function.
authors.affiliation: First Department of Surgery, Kagawa Medical School, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hamamoto
authors.forename: M A
authors.initials: MA
authors.lastname: Hossain
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Maeba
authors.forename: H
authors.initials: H
authors.lastname: Maeta
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D001646:Bile / Q000378:metabolism; D018395:CA-19-9 Antigen; D002448:Cell Adhesion; D003080:Cold Temperature; D019040:E-Selectin / Q000276:immunology / Q000502:physiology*; D005978:Glutathione; D007328:Insulin; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000502:physiology*; D007511:Ischemia; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019041:L-Selectin / Q000276:immunology / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000502:physiology; D008297:Male; D008833:Microcirculation; D009336:Necrosis; D009844:Oligosaccharides / Q000276:immunology / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D015424:Reperfusion / Q000295:instrumentation; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D000080506:Sialyl Lewis X Antigen; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D018395:CA-19-9 Antigen; D019040:E-Selectin; D007328:Insulin; D016169:Lymphocyte Function-Associated Antigen-1; D009844:Oligosaccharides; D019188:Organ Preservation Solutions; D000080506:Sialyl Lewis X Antigen; D014409:Tumor Necrosis Factor-alpha; C055206:University of Wisconsin-lactobionate solution; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D000493:Allopurinol; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose