Rhesus D genotyping in amniotic fluid.
Rhesus D genotyping by PCR of amniotic fluid offers a very valuable tool for prenatal care of Rhesus D-alloimmunized women. Misleading results have been reported for the method of Bennett et al. analysing the 3'-terminal end of the RhD gene. We have observed also problems with Rhesus D genotyping of DVI-variant samples in the assay of Simsek et al. utilizing size differences of intron 4 between the Rhesus D and the Rhesus CcEe gene. We, thus, prefer to amplify two different regions including the 3'-terminal region of the Rhesus D gene to further minimize the very low risk of false-negative results. This rapid Rhesus D genotyping usually does not require more than 2 ml of amniotic fluid.
Müller
T H
TH
DRK-Blutspendedienst Niedersachsen, Sachsen, Institut Oldenburg, Germany.
Gran
S
S
Lammers
I
I
Schunter
F
F
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000378:metabolism; D004899:Erythroblastosis, Fetal / Q000235:genetics* / Q000517:prevention & control; D005260:Female; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D018029:Rho(D) Immune Globulin; D012307:Risk Factors
D007518:Isoantibodies; C061961:RHO(D) antibody; D018029:Rho(D) Immune Globulin