Liver and intestinal fatty acid binding proteins in control and TGF beta 1 gene targeted deficient mice.
The effect of transforming growth factor beta-1 (TGF beta 1) expression on fatty acid binding proteins was examined in control and two strains of gene targeted TGF beta 1-deficient mice. Homozygous TGF beta 1-deficient 129 x CF-1, expressing multifocal inflammatory syndrome, had 25% less liver fatty acid binding protein (L-FABP) when compared to control mice. The decrease in L-FABP expression was not due to multifocal inflammatory syndrome since homozygous TGF beta 1-deficient/immunodeficient C3H mice on a SCID background had 36% lower liver L-FABP than controls. This effect was developmentally related and specific to liver, but not the proximal intestine, where L-FABP is also expressed. Finally, the proximal intestine also expresses intestinal-FABP (I-FABP) which decreased 3-fold in the TGF beta 1-deficient/immunodeficient C3H mice only. Thus, TGF beta 1 appears to regulate the expression of L-FABP and I-FABP in the liver and the proximal intestine, respectively.
Fontaine
R N
RN
Department of Veterinary Pathobiology, Texas A & M University, College Station 77843-4467, USA.
Gossett
R E
RE
Schroeder
F
F
O'Toole
B A
BA
Doetschman
T
T
Kier
A B
AB
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism; D017353:Gene Deletion; D018390:Gene Targeting; D007249:Inflammation / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D016513:Mice, SCID; D018996:Myelin P2 Protein / Q000096:biosynthesis*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016511:Severe Combined Immunodeficiency / Q000378:metabolism; D013577:Syndrome; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000502:physiology*; D019282:Wasting Syndrome / Q000378:metabolism
D002352:Carrier Proteins; C493320:Fabp1 protein, mouse; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016212:Transforming Growth Factor beta