Model systems for flavoenzyme activity. The effects of specific hydrogen bonds on the 13C and 1H NMR of flavins.
We have developed a family of receptors designed to bind flavin derivatives using specific hydrogen bond interactions. These synthetic host molecules provide a model for specific flavoenzyme-cofactor interactions, allowing isolation and observation of the effects of hydrogen bonding on flavin NMR. We describe here the use of one of these receptors to study the effects of hydrogen bonding to O(2), N(3), and O(4) on flavin 1H and 13C NMR.
Niemz
A
A
Department of Chemistry, University of Massachusetts, Amherst 01003, USA.
Rotello
V M
VM
D001665:Binding Sites; D002247:Carbon Isotopes; D005415:Flavins / Q000737:chemistry* / Q000378:metabolism*; D005420:Flavoproteins / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008958:Models, Molecular; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D014316:Tritium
D002247:Carbon Isotopes; D005415:Flavins; D005420:Flavoproteins; D014316:Tritium; D009247:NADH, NADPH Oxidoreductases