title: Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver.
abstract: The present study was performed to investigate processes involved in circumvention of the immune system by advanced stages of tumor growth in the liver. The efficacy of Kupffer cells and pit cells against cancer cells was tested in vivo in an experimental model of colon carcinoma metastasis in rat liver. Liver tumors were induced by administration of CC531 colon cancer cells into the vena portae. After 3 weeks, livers were obtained and partly fixed for electron microscopic procedures or frozen in liquid nitrogen for enzyme and immunohistochemistry at the light microscope level. The activation status of Kupffer cells was studied by expression of Ia-antigen (MHC class II) and by measurement of glucose-6-phosphate dehydrogenase (G6PDH) activity in the cells in situ as a measure of production of reactive oxygen species. Large numbers of Kupffer cells were found in liver parenchyma surrounding colon carcinomas when compared with levels in control livers, but these cells were not activated. Large numbers of activated monocytes and macrophages, cytotoxic T cells but only a few pit cells were found to be recruited to the boundary between liver parenchyma and tumors or their stroma. In those areas where cancer cells invaded liver parenchyma, only newly recruited macrophages and some Kupffer cells were present but few cytotoxic T cells or pit cells were found. The low activation status of Kupffer cells both in terms of production of reactive oxygen species and Ia-antigen expression and the absence of significant numbers of pit cells at tumor sites suggest that Kupffer cells and pit cells do not play a significant role in advanced stages of tumor growth. High levels of prostaglandin E2 were detected in the parenchyma of livers containing tumors and transforming growth factor beta was detected in the stroma of the tumors, therefore suggest that cytotoxicity of newly recruited monocytes, macrophages and cytotoxic T cells may be limited in these stages because of local production of these immunosuppressive factors.
authors.affiliation: Department of Animal Biology and CNR Center for Histochemistry, University of Pavia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Griffini
authors.forename: S M
authors.initials: SM
authors.lastname: Smorenburg
authors.forename: I M
authors.initials: IM
authors.lastname: Vogels
authors.forename: W
authors.initials: W
authors.lastname: Tigchelaar
authors.forename: C J
authors.initials: CJ
authors.lastname: Van Noorden
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology / Q000556:secondary*; D003110:Colonic Neoplasms / Q000473:pathology*; D015232:Dinoprostone / Q000032:analysis; D004195:Disease Models, Animal; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D007728:Kupffer Cells / Q000276:immunology / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000556:secondary*; D008264:Macrophages / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D009000:Monocytes / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D016212:Transforming Growth Factor beta; D015232:Dinoprostone