Kinetics of generation and persistence on membrane class II molecules of a viral peptide expressed on foreign and self proteins.
We had previously shown that a genetically engineered Ig expressing an immunodominant CD4+ T epitope corresponding to the 110-120 amino acids of influenza virus hemagglutinin (HA), activated T cells more efficiently than the synthetic peptide. Taking advantage of a T cell hybridoma specific for HA 110-120 and transfected with a reporter gene under the IL-2 promoter, we studied the kinetics of generation and persistence on surface MHC-II of the HA 110-120 peptide derived from various carriers. Our results show that the generation rate of immunogenic MHC II-peptide complex is dependent on the nature of the carrier. Abs specific for HA 110-120 peptide or for other epitopes on HA affect through various mechanisms the presentation of HA 110-120 peptide to T cells. The persistence of immunogenic MHC II-peptide complex on the surface of APC is not dependent on the nature of the carrier and correlates with the half-life of class II molecules, suggesting an irreversible binding of peptides to MHC-II molecules in 2PK3 murine B lymphoma cells.
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Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA. bot@msvax.mssm.edu
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Antohi
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Karjalainen
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D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D006825:Hybridomas; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015202:Protein Engineering; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D000906:Antibodies; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments