title: Haptoglobin concentration in serum and other body fluids measured by comparison of its reactivity with hemoglobin and concanavalin A.
abstract: Haptoglobin (Hp) concentration in normal human sera was determined either by means of the reaction with hemoglobin (Hb) or by reaction with the lectin concanavalin A (Con A). The ratio of Hp-Con A (1.02 +/- 0.68 g/l) to Hp-Hb (1.10 +/- 0.58 g/l) equal 0.96 +/- 0.32 was found to be characteristic for sera from healthy subjects. Measurements were also carried out for some pathological body fluids. Values of the ratio Hp-Con A/Hp-Hb in ascitic fluid, pleural effusion and cerebrospinal fluid were 1.72 +/- 1.13, 0.42 +/- 0.23 and 0.15 +/- 0.18. respectively. Haptoglobin present in urine and saliva was not recognized by Con A, whereas could form a complex with hemoglobin. Hp concentration determined by this method reached 2 mg/l.
authors.affiliation: Department of Chemistry, Medical Academy, Wrocław, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Katnik
authors.forename: J
authors.initials: J
authors.lastname: Jadach
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D003208:Concanavalin A / Q000378:metabolism; D006242:Haptoglobins / Q000032:analysis* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D011485:Protein Binding
chemical_list: D006242:Haptoglobins; D006454:Hemoglobins; D003208:Concanavalin A