The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts.
MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10(-12)-10(-6) M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD10/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stromal osteoblast cells.
Shamay
A
A
Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel.
Knopov
V
V
Benayahu
D
D
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism*; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology*; D017403:In Situ Hybridization; D051379:Mice; D015260:Neprilysin / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D004958:Estradiol; D000469:Alkaline Phosphatase; D015260:Neprilysin