title: Genotyping of hepatitis C virus isolates from Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis of 5' noncoding region.
abstract: We have genotyped the 5' noncoding region of hepatitis C virus in Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis. Of 50 patients, 15 had the expected 268-bp amplicon by reverse transcription-PCR. Specificity of the amplicons was confirmed by Southern hybridization. Restriction analysis of the amplicons showed the pattern for genotype 4 (common in the Middle East).
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, American University of Beirut, New York, New York 10022, USA. gmatar@layla.aub.ac.lb
authors.forename: G M
authors.initials: GM
authors.lastname: Matar
authors.forename: H M
authors.initials: HM
authors.lastname: Sharara
authors.forename: G E
authors.initials: GE
authors.lastname: Abdelnour
authors.forename: A M
authors.initials: AM
authors.lastname: Abdelnoor
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007861:Lebanon; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral