Effect of vitamin E on human aortic endothelial cell responses to oxidative injury.
Reactive oxygen species produced by the cells present in the arterial wall may cause oxidative damage to cellular components altering endothelial cell (EC) function. Changes in the EC function appear to play a key role in the pathogenesis of atherosclerosis. Human aortic endothelial cells (HAEC) were employed to investigate the protective role of vitamin E upon exposure of endothelial cells to oxidative stress in vitro. HAEC assimilate d-alpha-tocopherol from the media in a dose-dependent manner. Exposure of HAEC to 16.5 mM of the free radical generator 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH) for 16 h decreased cell viability (assessed by trypan blue exclusion) from 90 to 28%. HAEC preincubated with vitamin E at 15, 30, and 60 microM prior to the AAPH exposure resulted in a dose-dependent increase in resistance to oxidative stress and increased cell viability by 37, 66, and 85%, respectively. An increase in prostacyclin (PGI2) production by HAEC in response to AAPH exposure was correlated positively with cell damage and negatively with vitamin E concentration. Interleukin (IL)-1 production also increased in parallel with cell damage induced by AAPH. Vitamin E treatment significantly reduced IL-1 production after AAPH exposure. This modulatory role of vitamin E on HAEC function following exposure to an oxidative stress may reflect its antioxidant protection against lipid peroxidation.
Martin
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A
Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
Wu
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D
Baur
W
W
Meydani
S N
SN
Blumberg
J B
JB
Meydani
M
M
D000578:Amidines / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D011464:Epoprostenol / Q000378:metabolism; D005609:Free Radicals; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D007700:Kinetics; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D000578:Amidines; D000975:Antioxidants; D005609:Free Radicals; D007375:Interleukin-1; D017382:Reactive Oxygen Species; D014810:Vitamin E; C046728:2,2'-azobis(2-amidinopropane); D011464:Epoprostenol