A sensitive assay for the aminoimidazole-containing drug GP531 in plasma using liquid chromatography with amperometric electrochemical detection: a new class of electroactive compounds.
Aminoimidazole-containing compounds have been found to be electroactive and can be detected by amperometric electrochemical detection (ECD) with a high degree of sensitivity. A liquid chromatography (LC) method using ECD was developed for measuring plasma concentrations of the aminoimidazole-containing drug GP531, a potent adenosine-regulating agent. Plasma samples were extracted with 2-propanol and analyzed by LC under isocratic conditions using a mobile phase of methanol-sodium phosphate (pH 6.3; 3.3 mM) (32:68, v/v). The potential of the glassy carbon working electrode was set at +800 mV. The limit of quantitation was 12.5 ng ml-1 of GP531 using 100 microliters of plasma. The method was used to define the pharmacokinetics of GP531 in monkey following i.v. administration.
Chen
L S
LS
Department of Pharmacokinetics and Drug Metabolism, Gensia, Inc., San Diego, California 92121, USA.
Fujitaki
J M
JM
Dixon
R
R
D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid; D003853:Deoxyribonucleosides / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D004563:Electrochemistry; D000882:Haplorhini; D051381:Rats; D012680:Sensitivity and Specificity
D003853:Deoxyribonucleosides; C103214:GP 531; D000620:Aminoimidazole Carboxamide