Enhancement of in vitro renaturation of recombinant human pro-urokinase by ampicillin.
Ampicillin was used in in vitro renaturation process of recombinant human prourokinase and it can obviously improve the yield of the renaturation, although not as well as arginine. When ampicillin was used together with arginine or lysine, it decreased the yield of the renaturation comparing with the yield when arginine or lysine used alone.
Hua
Z C
ZC
Department of Biochemistry, Nanjing University, P. R. China.
Dong
C
C
Zhu
D X
DX
D000667:Ampicillin / Q000494:pharmacology*; D001120:Arginine; D004792:Enzyme Precursors / Q000737:chemistry*; D006801:Humans; D008239:Lysine; D011489:Protein Denaturation / Q000187:drug effects; D011994:Recombinant Proteins / Q000737:chemistry; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry*
D004792:Enzyme Precursors; D011994:Recombinant Proteins; D000667:Ampicillin; D001120:Arginine; D014568:Urokinase-Type Plasminogen Activator; D008239:Lysine