Nuclear LIM interactor, a rhombotin and LIM homeodomain interacting protein, is expressed early in neuronal development.
LIM domain-containing transcription factors, including the LIM-only rhombotins and LIM-homeodomain proteins, are crucial for cell fate determination of erythroid and neuronal lineages. The zinc-binding LIM domains mediate protein-protein interactions, and interactions between nuclear LIM proteins and transcription factors with restricted expression patterns have been demonstrated. We have isolated a novel protein, nuclear LIM interactor (NLI), that specifically associates with a single LIM domain in all nuclear LIM proteins tested. NLI is expressed in the nuclei of diverse neuronal cell types and is coexpressed with a target interactor islet-1 (Isl1) during the initial stages of motor neuron differentiation, suggesting the mutual involvement of these proteins in the differentiation process. The broad range of interactions between NLI and LIM-containing transcription factors suggests the utilization of a common mechanism to impart unique cell fate instructions.
Jurata
L W
LW
Biomedical Sciences Graduate Program, University of California at San Diego, La Jolla 92093-0650, USA.
Kenny
D A
DA
Gill
G N
GN
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005982:Glutathione Transferase; D006801:Humans; D060588:LIM Domain Proteins; D008667:Metalloproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D013937:Thymidine Kinase; D014157:Transcription Factors; D014162:Transfection; D015032:Zinc / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C065072:LMO1 protein, human; C085117:LMO2 protein, human; C513044:Lmo1 protein, mouse; C513188:Lmo2 protein, mouse; D008667:Metalloproteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D005982:Glutathione Transferase; D013937:Thymidine Kinase; D015032:Zinc