title: Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth.
abstract: We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth.
authors.affiliation: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Duina
authors.forename: J A
authors.initials: JA
authors.lastname: Marsh
authors.forename: R F
authors.initials: RF
authors.lastname: Gaber
mesh_terms: D000591:Amino Acid Isomerases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003001:Cloning, Molecular / Q000379:methods; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins; D005656:Fungal Proteins / Q000378:metabolism; D006655:Histone Deacetylases; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism; D000097322:Peptidyl-Prolyl Isomerase D
chemical_list: D000591:Amino Acid Isomerases; D002352:Carrier Proteins; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins; D005656:Fungal Proteins; D006655:Histone Deacetylases; D019696:Peptidylprolyl Isomerase; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C000631444:CPR6 protein, S cerevisiae; D000097322:Peptidyl-Prolyl Isomerase D; C000631445:CPR7 protein, S cerevisiae; C071665:RPD3 protein, S cerevisiae