The isolation of peste des petits ruminants virus from northern India.
The aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern India was confirmed as peste des petits ruminants virus. To differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture ELISA, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. The virulence profile of one isolate in cattle sheep and goats was established. Infected animals developed specific antibody responses and excreted specific antigen in their lachrymal secretions.
Nanda
Y P
YP
National Project for Rinderpest Eradication, Department of Animal Husbandry and Dairying, New Delhi, India.
Chatterjee
A
A
Purohit
A K
AK
Diallo
A
A
Innui
K
K
Sharma
R N
RN
Libeau
G
G
Thevasagayam
J A
JA
Brüning
A
A
Kitching
R P
RP
Anderson
J
J
Barrett
T
T
Taylor
W P
WP
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005455:Fluorescent Antibody Technique; D015511:Goat Diseases; D006041:Goats; D007194:India; D008969:Molecular Sequence Data; D018185:Morbillivirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D018107:Peste-des-petits-ruminants virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012756:Sheep; D012757:Sheep Diseases
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017931:DNA Primers