Determination of benzo[a]pyrene in lipid-soluble liquid smoke (LSLS) by HPLC-FL.
We developed a method for the determination of low levels of the carcinogen benzo[a]pyrene (BaP) in lipid-soluble liquid smoke (LSLS). The method consists of partitioning the LSLS (2 g) between n-hexane and DMSO, purification of the DMSO extracts on Sep-Pak C18 Plus and then Silica Plus cartridges, and determination of the BaP in the isolated extract by HPLC with fluorescence detection. Detection and quantification limits were 0.049 microgram/l (0.024 microgram/kg of LSLS) and 0.089 microgram/l (0.045 mg/kg), respectively. Recovery (87%) and CV% (< or = 1.5) were satisfactory.
García Falcón
M S
MS
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, Spain.
González Amigo
S
S
Lage Yusty
M A
MA
López de Alda Villaizán
M J
MJ
Simal Lozano
J
J
D001564:Benzo(a)pyrene / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005421:Flavoring Agents / Q000032:analysis*; D012906:Smoke
D002273:Carcinogens; D005421:Flavoring Agents; D012906:Smoke; D001564:Benzo(a)pyrene