DNA adducts in environmental, occupational and life-style studies in human biomonitoring.
The importance of DNA adducts in carcinogenesis had been discussed. The 32P-postlabelling method was developed as a quantitative technique to measure the level of different DNA adducts including adducts in human DNA. The elevated level of DNA adducts was found in white blood cells in persons exposed environmentally and occupationally to high concentrations of PAHs (polycyclic aromatic hydrocarbons) in the ambient air. Tobacco also generated higher level of DNA adducts both in lymphocytes and laryngeal tissues of smokers. Exposure to styrene has been of interest world-wide because of the very high exposure and persistence of adducts in DNA of lamination workers.
Hemminki
K
K
Department of Bioscience at Novum, Karolinska Institute, Huddinge, Sweden.
Grzybowska
E
E
Widłak
P
P
Chorazy
M
M
D000393:Air Pollutants; D000395:Air Pollutants, Occupational; D018736:DNA Adducts / Q000097:blood*; D004784:Environmental Monitoring; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008019:Life Style; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D020058:Styrene; D013343:Styrenes / Q000009:adverse effects
D000393:Air Pollutants; D000395:Air Pollutants, Occupational; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D013343:Styrenes; D020058:Styrene