Collagen content in rat liver after experimentally induced cholestasis followed by choledochojejunostomy and X-irradiation.
The right part of the median lobe of the liver of female Wistar rats was irradiated, 12.5 or 25 Gy, at a field size of 15 x 20 mm. The central part of the irradiated liver lobe was fixed and used for the estimation of the collagen protein ratio by means of the Sirius Red-Fast Green extraction method, immediately, 8, 16 or 32 weeks after irradiation. No significant increase in collagen content could be demonstrated in this time range, both after irradiation at 12.5 Gy and at 25 Gy. Partial hepatectomy according to Higgins led to rapid regrowth of the remaining liver lobes. The right lobe grew out rapidly to replace the median lobe. Two days after partial hepatectomy the right lobe was irradiated at the same field size. Measurement of the collagen protein ratio in this experiment did not show a significant increase 8, 16 or 32 weeks after irradiation. However, the 25 Gy group did not survive long enough to obtain data at 16 or 32 weeks. The animals in this latter experiment suffered from ascites before dying. Experimentally induced cholestasis was obtained by ligation and partial resection of the common bile duct. After two weeks of cholestasis the bile flow was restored by Roux-en-Y choledochojejunostomy. The effect of irradiation 2 days after repair surgery was studied. Without irradiation the collagen protein ratio is increased. Irradiation of the right part of the median lobe led to a relatively enhanced collagen content in this lobe. Our results indicate that radiation itself does not lead to a significantly enhanced degree of fibrosis in the liver. However when an increase in collagen content was induced by cholestasis, the partial "dilution" of enhanced fibrosis as a result of proliferation of liver parenchyma cells following repair surgery was inhibited by irradiation.
Haveman
J
J
Department of Radiotherapy, University of Amsterdam, The Netherlands.
James
J
J
Geerdink
A
A
D000818:Animals; D001201:Ascites / Q000378:metabolism; D002768:Choledochostomy; D002779:Cholestasis / Q000378:metabolism* / Q000401:mortality; D003094:Collagen / Q000378:metabolism*; D005260:Female; D005355:Fibrosis / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000293:injuries / Q000378:metabolism* / Q000601:surgery; D008115:Liver Regeneration / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014965:X-Rays / Q000009:adverse effects
D003094:Collagen