Detection of Escherichia coli O157:H7 in water from coliform enrichment cultures.
Environmental water samples were seeded with Escherichia coli O157:H7 and the bacterium was recovered using a traditional coliform enrichment procedure followed by selective plating on sorbitol MacConkey agar and biochemical and serological characterization. Assays for beta-glucuronidase and glutamate decarboxylase were found to be useful procedures for screening suspected isolates. The organism was not recovered in a survey of various water samples.
Rice
E W
EW
US Environmental Protection Agency, Cincinnati, OH 45268, USA.
Johnson
C H
CH
Reasoner
D J
DJ
D001431:Bacteriological Techniques; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005966:Glucuronidase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D012703:Serotyping; D014871:Water Microbiology
D003470:Culture Media; D005966:Glucuronidase; D005968:Glutamate Decarboxylase