Anti-IRS-1 monoclonal antibody, 6G5, cross-reacts to IRS-2.
We previously reported the production of anti-IRS-1 monoclonal antibodies against human IRS-1 C-terminal portion. One of anti IRS-1 monoclonal antibodies, 6G5, was found to immunologically cross-react with IRS-2, which has been reported recently. The data presented here provide information arising from the use of the anti-IRS-1 MAb, 6G5; this MAb was found to be a useful reagent in studying the functional role of IRSs in the insulin-signaling system.
Yamauchi
T
T
Department of Biochemistry II, Daisan Hospital, Jikei University School of Medicine, Tokyo, Japan.
Nishimaki
H
H
Furusaka
A
A
Tanaka
T
T
Hayashi
S
S
Tamemoto
H
H
Taylar
S
S
Tobe
K
K
Kadowaki
T
T
Nishiyama
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases; D010748:Phosphopeptides / Q000276:immunology; D010750:Phosphoproteins / Q000172:deficiency / Q000276:immunology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; C526292:IRS1 protein, human; C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; C526295:Irs1 protein, mouse; C526298:Irs2 protein, mouse; D010748:Phosphopeptides; D010750:Phosphoproteins; D011994:Recombinant Proteins; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor)