The gene encoding the catalytic subunit C alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1.
We have determined the chromosomal localization of the gene for the catalytic subunit C alpha of cAMP-dependent protein kinase (locus PRKACA) to human chromosome 19 using polymerase chain reaction (PCR) and Southern blot analysis of two different somatic cell hybrid mapping panels. In addition, PCR analysis of a chromosome 19 mapping panel revealed the presence of a human C alpha-specific amplification product only in cell lines containing the region 19p13.1 to 19q12. Finally, two-color fluorescence in situ hybridization to metaphase chromosomes using the human C alpha cDNA and human chromosome 19 inter-Alu-PCR product as probes localized the human C alpha gene to chromosome region 19p13.1.
Taskén
K
K
Institute of Medical Biochemistry, University of Oslo, Norway. kjetil.tasken@basalmed.uio.no
Solberg
R
R
Zhao
Y
Y
Hansson
V
V
Jahnsen
T
T
Siciliano
M J
MJ
D000818:Animals; D015139:Blotting, Southern; D002384:Catalysis; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D006224:Cricetinae; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
D017868:Cyclic AMP-Dependent Protein Kinases