Effects of glutamate receptor agonists on presumed presynaptic Ca(2+)-signals in juvenile rat hippocampal area CA1.
The physiological role of presynaptic glutamate receptors in controlling presynaptic Ca(2+)-influx and thereby transmitter release is unknown. To test if presynaptic Ca(2+)-uptake in the hippocampus is controlled by glutamate autoreceptors, we created a hippocampal slice preparation for investigation of presumed presynaptic Ca(2+)-signals with ion-sensitive microelectrodes after lesioning of post-synaptic neurons by glucose deprivation. After prolonged glucose deprivation in slices from juvenile animals of postnatal days 13-15 and 20-22, stratum radiatum (SR) and alveus stimulation-induced postsynaptic field potential (fp) components were irreversibly abolished in area CA1, whereas SR stimulation still evoked afferent volleys. Repetitive stimulation of the SR still induced small decreases in the extracellular Ca2+ concentration ([Ca2+]o), but repetitive alveus stimulation no longer induced decreases in [Ca2+]o, suggesting a complete damage of pyramidal cells. In lesioned slices the remaining SR stimulation-induced small decreases in [Ca2+]o presumably reflect presynaptic Ca(2+)-influx. These small decreases in [Ca2+]o were reversibly reduced by kainate, RS-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), N-methyl-D-aspartate (NMDA) and L-glutamic acid (glutamate), without effects on afferent volleys.
Alici
K
K
Department of Neurophysiology, Medical School of the Humboldt University Berlin, Germany. alici@rz.charite.hu-berlin.de
Müller
W
W
Heinemann
U
U
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002118:Calcium / Q000502:physiology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000187:drug effects*; D013569:Synapses / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D017470:Receptors, Glutamate; D005947:Glucose; D007608:Kainic Acid; D002118:Calcium