The effects of temperature on the filtration of diluted blood through 3 mu m and 5 mu m filters.
The effect of temperature on the flow of diluted blood [Hct = 0.21], through 5 mu m Nuclepore filters, is described by the Arrhenius equation with an energy of activation of 27.7 kJ/mol. Plasma, diluted with PBS, is almost three times less sensitive to temperature, with an energy of activation of 9.8 kJ/mol, while red cells are of intermediate sensitivity, with an energy of activation of 14.7 kJ/mol. The most sensitive elements to changes in temperature are leukocytes, with energies of activation of 31 kJ/mol and 35 kJ/mol for fast-flowing leukocytes (granulocytes and lymphocytes) and slow-flowing leukocytes (monocytes) respectively. Hence, the major determinants of the decline in filterability of blood through micropore filters are the leukocytes. This effect is compounded when blood is kept for 10 minutes or more at 10 degrees C due to activation of granulocytes, which leads to permanent pore blocking when the affected blood is filtered at room temperature. The combination of increased passage time of leukocytes through peripheral areas at abnormally low temperatures and subsequent activation might influence the flow of blood in non-affected tissues. The effect of temperature on the filterability of red blood cells through 3 mu m filters is not described by the Arrhenius equation and the deviations are seen as a gradual change of slope rather than a sharp break between two straight lines. The data are consistent with a gradual shift in rate limiting step away from the entry event into pores, which dominates at low temperature but becomes progressively less important at elevated temperatures. The changing parameter is probably the volume of the red cell, which is less important when flow is measured through 5 mu m pores.
Adams
R A
RA
School of Molecular and Medical Biosciences, Cardiff, UK.
Evans
S A
SA
Kooshesh
F
F
Jones
J G
JG
D004912:Erythrocytes / Q000502:physiology; D005374:Filtration; D018056:Hemorheology; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D010949:Plasma / Q000502:physiology; D013696:Temperature