CD30 expression on human CD8+ T cells isolated from peripheral blood lymphocytes of normal donors.
By direct or indirect immunofluorescence using FITC as a fluorochrome, 0 to 2% of the T cells isolated from peripheral blood of normal healthy individuals was found to be CD30+. Using indirect immunofluorescence and phycoerythrin-labeled Ab, higher percentages of CD30+ T cells (3-31%) were repeatedly found in the peripheral blood of normal healthy donors. The majority (85-90%) of CD30+ T cells obtained from PBLs of normal healthy donors were found in the CD8+ cell population. Following FACS sorting of the T cell populations into CD30+ and CD30- subpopulations, approximately 85 to 90% CD30+ T cells were CD8+, whereas the CD30- T cells were CD4+. Upon activation, the sorted CD30+ T cells produced both IFN-gamma and IL-4. In contrast to previous reports, these results demonstrate that CD30+CD8+ T cells are present in significant numbers in the PBLs of normal healthy individuals.
Agrawal
B
B
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
Reddish
M
M
Longenecker
B M
BM
D000906:Antibodies / Q000494:pharmacology; D017252:CD3 Complex; D018414:CD8-Positive T-Lymphocytes / Q000145:classification / Q000276:immunology*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis; D017730:Ki-1 Antigen / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology; D010799:Phycoerythrin; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000906:Antibodies; D017252:CD3 Complex; D005456:Fluorescent Dyes; D007376:Interleukin-2; D017730:Ki-1 Antigen; D010799:Phycoerythrin; D015847:Interleukin-4; D007371:Interferon-gamma; D016650:Fluorescein-5-isothiocyanate