title: Povidone-iodine (betadine) in the treatment of experimental Pseudomonas aeruginosa keratitis.
abstract: Topical 5% povidone-iodine for the treatment of corneal ulcers was observed in Sierra Leone, West Africa by one of us (D.J.D.). To test the efficacy of topical 5% povidone-iodine for infectious keratitis, experimental Pseudomonas aeruginosa keratitis was induced in 12 rabbits by first abrading the central 3 mm of corneal epithelium. Thirty milliliters of broth of P. aeruginosa strain ATCC 27835 (1.8 x 10(7) viable bacteria) was dropped twice on the wounded cornea. After 22 h, all corneas were clinically infected. Eight rabbits were treated with 5% povidone-iodine solution and four with 0.9% NaCl solution. All were given hourly drops. Twenty-four hours after treatment began, the central 8-mm button of the infected cornea was excised, homogenized, and serial dilutions plated onto MacConkey agar. The total number of viable Pseudomonas organisms was calculated. The treatment group had 5.2 +/- 0.4 CFUs (colony-forming units) per cornea. The control group had 4.8 +/- 0.4 CFUs per cornea (p = 0.11). The clinical scores (Hobden grading system) were 6.9 +/- 1.5 for the treated group and 7.3 +/- 2.5 for the control group (p = 0.74). There was no statistical difference between the treated and control groups. Povidone-iodine (5%) is not effective in the acute treatment of P. aeruginosa keratitis in this rabbit model.
authors.affiliation: Department of Ophthalmology, University of Minnesota Hospital and Clinic, Minneapolis 55455-0501, USA.
authors.forename: K
authors.initials: K
authors.lastname: Michalová
authors.forename: A L
authors.initials: AL
authors.lastname: Moyes
authors.forename: S
authors.initials: S
authors.lastname: Cameron
authors.forename: B A
authors.initials: BA
authors.lastname: Juni
authors.forename: W F
authors.initials: WF
authors.lastname: Obritsch
authors.forename: J A
authors.initials: JA
authors.lastname: Dvorak
authors.forename: D J
authors.initials: DJ
authors.lastname: Doughman
authors.forename: F S
authors.initials: FS
authors.lastname: Rhame
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D003315:Cornea / Q000187:drug effects / Q000382:microbiology; D003320:Corneal Ulcer / Q000188:drug therapy* / Q000382:microbiology; D004195:Disease Models, Animal; D015818:Eye Infections, Bacterial / Q000188:drug therapy* / Q000209:etiology; D009883:Ophthalmic Solutions; D011206:Povidone-Iodine / Q000008:administration & dosage*; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000209:etiology; D011550:Pseudomonas aeruginosa / Q000502:physiology; D011817:Rabbits
chemical_list: D000891:Anti-Infective Agents, Local; D009883:Ophthalmic Solutions; D011206:Povidone-Iodine