title: Otoconial alterations after embryonic development in hypergravity.
abstract: The relation between prolonged hypergravity and structural adaptation of otoconia was studied in hamsters (n = 56). Three groups of hamsters (n = 27), were conceived and born in a centrifuge: group 1 (n = 10) 1 month under 2.5 G, group 2 (n = 9) 5 months under 2.5 G and 4 months under 1 G, group 3 (n = 8) 1 month under 2.5 G and 8 months under 1 G. Control hamsters (n = 29) were conceived and born under 1 G (1 month old, n = 7; 9 months old, n = 22). Histological study of the otoconial layers (energy dispersive x-ray element analysis and scanning electron microscopy) showed similar calcium content, size, and shape in utricular and saccular otoconia in all groups. Different were the utricular otoconial size classes, large, medium-sized, and small. The area with small otoconia increased in group 1 (p = 0.002). In group 2, the large otoconial area decreased (p = 0.001) and the medium-sized one increased (p < 0.001). In group 3, the large otoconial area decreased (p = 0.003) and the medium-sized one increased (p = 0.007). For age-related effects we found group 1 with an increased area of large otoconia (p = 0.001) and a decreased medium-sized one compared to groups 2 (p < 0.001) and 3 (p = 0.02). Hypergravity during formation of otoconia does not affect calcium content, size, or shape, but changes relative size of the areas with large, medium-sized, or small otoconia and the development of these areas. This resulted in a structural adaptation to hypergravity.
authors.affiliation: Vestibular Department, University of Amsterdam, The Netherlands.
authors.forename: H N
authors.initials: HN
authors.lastname: Sondag
authors.forename: H A
authors.initials: HA
authors.lastname: De Jong
authors.forename: J
authors.initials: J
authors.lastname: Van Marle
authors.forename: B
authors.initials: B
authors.lastname: Willekens
authors.forename: W J
authors.initials: WJ
authors.lastname: Oosterveld
mesh_terms: D000818:Animals; D006224:Cricetinae; D005314:Embryonic and Fetal Development; D018471:Hypergravity; D008297:Male; D010037:Otolithic Membrane / Q000502:physiology*