Transcriptional regulatory elements in the rat bradykinin B2 receptor gene.
In an attempt to elucidate the mechanisms underlying the regulation of bradykinin B2 receptor gene expression, the molecular structure of the rat gene including the 5'-flanking region was characterized (J. Biol. Chem. 269: 26920-26925, 1994). In this study we show that the gene spans about 32 kb, including a long first intron of 25 kb. The promoter region drives reporter gene expression in NG108-15 neuroglioma cells, and its expression is upregulated by cAMP, bradykinin, phorbol esters and by coexpression of an activated ras oncogene but not by dexamethasone.
Pesquero
J B
JB
Department of Biophysics, Escola Paulista de Medicina, Säo Paulo, Brazil.
Lindsey
C J
CJ
Paiva
A C
AC
Ganten
D
D
Bader
M
M
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001920:Bradykinin / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D011905:Genes, ras; D006801:Humans; D007438:Introns; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D017931:DNA Primers; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013755:Tetradecanoylphorbol Acetate; D001920:Bradykinin