title: Circularly permuted dihydrofolate reductase possesses all the properties of the molten globule state, but can resume functional tertiary structure by interaction with its ligands.
abstract: It is obvious that functional activity of a protein molecule is closely related to its structure. On the other hand, the understanding of structure-function relationship still remains one of the intriguing problems of molecular biology. There is widespread belief that mutagenesis presents a real way to solve this problem. Following this assumption, we have investigated the effect of circular permutation in dihydrofolate reductase from E. coli on protein structure and functioning. It has been shown that in the absence of ligands two circularly permuted variants of dihydrofolate reductase possess all the properties of the molten globule state. However, after addition of ligands they gain the native-like structural properties and specific activity. This means that the in vitro folding of permuted dihydrofolate reductase is terminated at the stage of the molten globule formation. Interaction of permuted protein with ligands leads to the structural adjustment and formation of active protein molecules.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Moscow Region, Russia. uversky@sun.ipr.serpukhov.su
authors.forename: V N
authors.initials: VN
authors.lastname: Uversky
authors.forename: V P
authors.initials: VP
authors.lastname: Kutyshenko
authors.lastname: Protasova NYu
authors.forename: V V
authors.initials: VV
authors.lastname: Rogov
authors.forename: K S
authors.initials: KS
authors.lastname: Vassilenko
authors.forename: A T
authors.initials: AT
authors.lastname: Gudkov
mesh_terms: D000818:Animals; D002151:Calorimetry; D018834:Chaperonin 60 / Q000378:metabolism; D002942:Circular Dichroism; D004926:Escherichia coli / Q000201:enzymology; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D016296:Mutagenesis; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology
chemical_list: D018834:Chaperonin 60; D008024:Ligands; D014508:Urea; D013762:Tetrahydrofolate Dehydrogenase