title: Development of chiral high performance liquid chromatographic assays for the enantiomers of 8-hydroxy-(di-n-propylamino)tetralin and three 8-keto-pyrrole-substituted analogues.
abstract: Two chiral HPLC methods using protein-based stationary phases for the analysis of 8-hydroxy-(di-n-propylamino)tetralin and three 8-keto-pyrrole analogues are presented. Efficient solid-phase extraction enabled quantification of 0.02 microgram ml-1 of the 8-keto-pyrrole analogues from 500 microliters rat hepatocyte suspensions, and 0.2 microgram ml-1 to be measured from 50 microliters of rat plasma using UV detection at 315 nm.
authors.affiliation: Pharmacia & Upjohn Inc., Crawley, West Sussex, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Wood
authors.forename: S N
authors.initials: SN
authors.lastname: Pegg
authors.forename: R J
authors.initials: RJ
authors.lastname: Simmonds
authors.forename: D
authors.initials: D
authors.lastname: Stevenson
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin