Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.
Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infected cell suspensions and culture supernatants were compared among seven assays sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log10 virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistently low titers.
Scott
W A
WA
Department of Biochemistry & Molecular Biology, University of Miami, Florida 33101, USA. wscott@mednet.med.miami.edu
Brambilla
D
D
Siwak
E
E
Beatty
C
C
Bremer
J
J
Coombs
R W
RW
Farzadegan
H
H
Fiscus
S A
SA
Hammer
S M
SM
Hollinger
F B
FB
Khan
N
N
Rasheed
S
S
Reichelderfer
P S
PS
D018929:Cell Culture Techniques; D015925:Cryopreservation; D015658:HIV Infections; D015497:HIV-1; D006801:Humans; D013997:Time Factors