Testicular gamma-aminobutyric acid and circulating androgens in Syrian and Djungarian hamsters during sexual development.
Several factors, besides luteinizing hormone (LH), participate in the modulation of testicular function. A number of neurotransmitters are reported to be involved in this process, including a stimulatory action of gamma-aminobutyric acid (GABA) on steroidogenesis in the rat testis. The purpose of this study was to investigate the testicular pattern of GABA and glutamic acid, one of its main precursors, during sexual maturation in two seasonally breeding species: Syrian (golden) and Djungarian hamsters. Plasma androgen levels were also measured. The animals were maintained under long-day photoperiod (16:8, L:D) and were killed at 23, 30, 36, 46, 60, and 90 days of age. A different pattern of developmental changes in body and testicular weight was observed in these two species. GABA was present in the testes at all ages studied. GABA concentration and content showed a sharp elevation in the prepubertal period in golden as well as Djungarian hamsters. However, glutamic acid concentrations remained nearly constant during development in both species. Glutamic acid content increased gradually with age in the golden hamster, while a marked peak at 36 days of age was detected in the Djungarian hamster. Plasma testosterone and dihydrotestosterone levels were maximal at pubertal age in both species. The plasma levels of 5 alpha-androstane-3 alpha, 17 beta-diol increased significantly at 30 days of age in the golden hamster while in Djungarian hamsters this steroid remained unchanged. These results suggest that glutamic acid may serve as a precursor for GABA biosynthesis in the testis. In addition, changes in testicular GABA and plasma androgen levels might reflect a modulatory effect of this neurotransmitter in the acquisition of steroidogenic capability during development.
Frungieri
M B
MB
Instituto de Biologia y Medicina Experimental, CONICET, Buenos Aires, Argentina.
Gonzalez-Calvar
S I
SI
Chandrashekar
V
V
Rao
J N
JN
Bartke
A
A
Calandra
R S
RS
D000728:Androgens / Q000097:blood*; D000818:Animals; D001835:Body Weight; D006224:Cricetinae; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D009929:Organ Size; D017111:Phodopus; D012741:Sexual Maturation; D013045:Species Specificity; D013737:Testis / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000728:Androgens; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid