title: Identification of a novel paternally expressed transcript adjacent to snRPN in the Prader-Willi syndrome critical region.
abstract: Small nuclear ribonucleoprotein-associated polypeptide N (snRPN) and an anonymous transcript, PAR-5, are two of the paternally expressed transcripts mapped to the Prader-Willi syndrome critical region. Using long-range PCR, we have isolated the genomic interval between snRPN and PAR-5, identified a novel transcript in this region, and termed it PAR-SN. Northern analysis demonstrates that PAR-SN is expressed in brain, skeletal muscle, and heart. Like snRPN and PAR-5, PAR-SN is expressed exclusively from the paternal homolog in cultured lymphoblasts. Sequence analysis of the transcript revealed no significant open reading frame but did include a polymorphic dinucleotide repeat (CA)17.
authors.affiliation: Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ning
authors.forename: A
authors.initials: A
authors.lastname: Roschke
authors.forename: S L
authors.initials: SL
authors.lastname: Christian
authors.forename: J
authors.initials: J
authors.lastname: Lesser
authors.forename: J S
authors.initials: JS
authors.lastname: Sutcliffe
authors.forename: D H
authors.initials: DH
authors.lastname: Ledbetter
mesh_terms: D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D011218:Prader-Willi Syndrome / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D017411:Ribonucleoproteins, Small Nuclear