Molecular characterization of the segment 2 gene of epizootic hemorrhagic disease virus serotype 2: gene sequence and genetic diversity.
The complete nucleotide sequence of the gene encoding the major outer capsid protein VP2 from the Alberta isolate of epizootic hemorrhagic disease virus serotype 2 (EHDV-2) was determined. Complementary DNA (cDNA) corresponding to segment 2 was 3002 nucleotides in length with a single open reading frame that encoded a VP2 of 982 amino acids. Although the VP2 from EHDV-2 was only 34% homologous to the cognate protein from EHDV-1, their predicted hydropathic profiles were similar, suggesting that conservation of structure is important biologically to these capsid proteins. Sequence analysis of six North American EHDV-2 field isolates showed a high degree of comparative genetic identity (> 97%). Phylogenetic profiles constructed suggest that regionalization of the viruses within the North American continent has contributed to the genetic diversity.
Cheney
I W
IW
Department of Molecular Biology, University of Wyoming, Laramie 82071, USA.
Yamakawa
M
M
Roy
P
P
Mecham
J O
JO
Wilson
W C
WC
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D014644:Genetic Variation; D018164:Hemorrhagic Disease Virus, Epizootic / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
D036022:Capsid Proteins; D012367:RNA, Viral