title: Improved yield of peripheral blood progenitor cells on the Cobe Spectra.
abstract: The efficiency of leucapheresis using the Cobe Spectra depends on the observation of the operator to monitor the haematocrit (Hct) in the collection line at the recommended Hct of 1%. Cells were harvested by processing 3 x total blood volume (TBV) over 3-4 h. During the harvest the manual Hct and the colony-forming units granulocyte-macrophage (CFU-GM) concentrations were analysed in the collection line in the observed Hct range of 1-7.5%. There was a correlation between the manual and observed Hct (r = 0.97). The CFU-GM concentration was normalised to allow comparison between patients. The increase was statistically significant between 1-2% (P = 0.04) and 1-3% Hct (P = 0.05). The increase in CFU-GM concentration remained at the termination of harvest, indicating that not all available CFU-GM were harvested. The optimum concentration of progenitor cells was found to be at 3% Hct. We postulate that this may permit the collection of sufficient cells on one occasion to allow PBPC autografting in the majority of patients who respond to mobilisation.
authors.affiliation: Department of Clinical Haematology, Addenbrooke's NHS Trust, Cambridge, UK.
authors.forename: H K
authors.initials: HK
authors.lastname: Jestice
authors.forename: P
authors.initials: P
authors.lastname: Boraks
authors.forename: M A
authors.initials: MA
authors.lastname: Scott
authors.forename: D
authors.initials: D
authors.lastname: Bloxham
authors.forename: J
authors.initials: J
authors.lastname: Price
authors.forename: R E
authors.initials: RE
authors.lastname: Marcus
mesh_terms: D000328:Adult; D005260:Female; D006400:Hematocrit; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis; D008297:Male