title: Adaptation of cholesterol absorption after proximal resection of porcine small intestine.
abstract: Cholesterol absorption occurs primarily in the upper small intestine. Our aim was to assess absorption of cholesterol during ileal adaptation after proximal small intestinal resection. In vivo absorption and elimination of cholesterol, plasma cholesterol, cholesterol precursors, and plant sterols were related to intestinal morphology and transit 4 (n = 5), 8 (n = 5), and 14 (n = 5) weeks after a 75% proximal resection of porcine small intestine, and compared to preoperative (n = 5) and transected (n = 5) control animals. Fractional cholesterol absorption, the daily amount of cholesterol absorbed, plasma cholesterol, and plant sterol to cholesterol proportions were significantly (P < 0.05 or less) decreased, whereas fecal loss of cholesterol as neutral steroids, less so as bile acids, plasma cholesterol precursor proportions, and ileal mass and villus height were significantly increased (P < 0.05 or less) after 8 weeks of the resection. Cholesterol absorption efficiency, decreased by the resection, was gradually increased from 5.4 +/- 2.2 to 26.9 +/- 3.9% during the 14 postoperative weeks (P < 0.0001) simultaneously with a 46% increase in villus height compared with transection (P < 0.0001), but absorption remained still below control levels (80.4 +/- 2.5%, P < 0.0001). In resected and control animals, villus height correlated positively with cholesterol absorption efficiency (r = 0.85, P < 0.0001; r = 0.76, P = 0.01) and plasma plant sterol proportions (r = 0.94-0.95, P < 0.0001; r = 0.78-0.85, P < 0.008), respectively. In conclusion, after massive proximal small bowel resection, adaptation of intestinal cholesterol absorption efficiency occurs in the distal ileum closely parlleling villus hypertrophy.
authors.affiliation: Department of Gastrointestinal Surgery, Helsinki University Central Hospital, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Pakarinen
authors.forename: T A
authors.initials: TA
authors.lastname: Miettinen
authors.forename: J
authors.initials: J
authors.lastname: Lauronen
authors.forename: P
authors.initials: P
authors.lastname: Kuusanmäki
authors.forename: P
authors.initials: P
authors.lastname: Raivio
authors.forename: T
authors.initials: T
authors.lastname: Kivistö
authors.forename: J
authors.initials: J
authors.lastname: Halttunen
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry; D001835:Body Weight; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000032:analysis / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D007082:Ileum / Q000378:metabolism* / Q000601:surgery; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000378:metabolism* / Q000601:surgery; D007583:Jejunum / Q000601:surgery; D011897:Random Allocation; D013552:Swine
chemical_list: D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D002784:Cholesterol