title: Immunobiological diagnosis of tropical ocular diseases: Toxocara, Pythium insidiosum, Pseudomonas (Burkholderia) pseudomallei, Mycobacterium chelonei and Toxoplasma gondii.
abstract: Of the sera obtained from 18 patients with ocular diseases comprising ocular larva migrans (OLM), Toxoplasma gondii, Mycobacterium chelonei, Pythium insidiosum bacteria and tumour, 3 sera were positive for Toxocara antibody at the titre over 1/1600 ELISA. All 3 of these sera came from males with unilateral grayish fundi. We demonstrated the value of the direct immunofluorescent assay (DIFA) on vitreous specimens from 7 cases of Toxoplasma retinitis. One vitreous specimen under electronmicroscopic examination revealed bradyzoites of T. gondii. In addition, a few cases of keratoconjunctivitis/ corneal ulcer with endophthalmitis, due to P. insidiosum, Ps. pseudomallei and Mycobacterium chelonei, were also diagnosed.
authors.affiliation: Mahidol University, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Srimuang
authors.forename: K
authors.initials: K
authors.lastname: Roongruangchai
authors.forename: T
authors.initials: T
authors.lastname: Lawtiantong
authors.forename: A D
authors.initials: AD
authors.lastname: Chusattayanond
authors.forename: S
authors.initials: S
authors.lastname: Warrasak
authors.forename: S
authors.initials: S
authors.lastname: Sahaphong
authors.forename: D
authors.initials: D
authors.lastname: Tanphaichitra
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D019121:Burkholderia Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005128:Eye Diseases / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis*; D016926:Mycobacterium chelonae; D011775:Pythium; D014120:Toxocariasis / Q000175:diagnosis*; D014123:Toxoplasmosis / Q000175:diagnosis*; D014330:Tropical Medicine