Expression of receptor protein-tyrosine phosphatase alpha mRNA and protein during mouse embryogenesis.
Receptor protein-tyrosine phosphatase alpha (RPTP alpha) is a transmembrane member of the family of protein-tyrosine phosphatases (PTPs) that has been implicated in neuronal differentiation in vitro. Here we demonstrate that RPTP alpha is differentially expressed during mouse embryogenesis in a spatio-temporal manner. RPTP alpha expression was detectable in 6 days post coitum (dpc) embryos, but not in 7.5 dpc embryos. From 10.5 dpc onwards a striking RPTP alpha expression pattern was observed with elevated levels in the dorsal root ganglia, cranial ganglia and adrenal gland, suggesting that RPTP alpha levels are specifically enhanced in neural crest derivatives. Marked differences between RPTP alpha mRNA and protein levels indicated that RPTP alpha expression is regulated by transcriptional and (post-) translational mechanisms. The expression pattern of RPTP alpha suggests that RPTP alpha may play a role in neural crest cell differentiation in vivo.
den Hertog
J
J
Hubrecht Laboratory, Netherlands Institute for Development Biology, Utrecht, The Netherlands. hertog@hubrecht.nl
Overvoorde
J
J
de Laat
S W
SW
D000367:Age Factors; D000818:Animals; D004622:Embryo, Mammalian / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D009024:Morphogenesis; D009432:Neural Crest / Q000737:chemistry; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017027:Protein Tyrosine Phosphatases