Age-related increase in oxidized proteins in mouse synaptic mitochondria.
Since it has been proposed that oxidized protein accumulation plays a critical role in brain aging, we have investigated their contents in synaptic mitochondria from five age groups of mice. Protein carbonyl content in synaptic mitochondria showed a significant positive correlation with age (r = 0.95, P = 0.01). A linear inverse relationship was observed between protein carbonyl content and complex IV/complex I ratio (which was used as an index of imbalance between mitochondrial respiratory complexes) in synaptic mitochondria in the five age groups (r = -0.99, P < 0.001). We suggest that age-related accumulation of oxidized proteins in synaptic mitochondria may be the result of an age-dependent increase in reactive oxygen species generation because of a disarrangement of mitochondrial oxidative phosphorylation.
Martinez
M
M
Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad de Alicante, Spain.
Hernández
A I
AI
Martínez
N
N
Ferrándiz
M L
ML
D000375:Aging / Q000378:metabolism*; D000447:Aldehydes / Q000032:analysis; D000818:Animals; D003576:Electron Transport Complex IV / Q000032:analysis; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis; D010084:Oxidation-Reduction; D013569:Synapses / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure*
D000447:Aldehydes; D016660:NAD(P)H Dehydrogenase (Quinone); D003576:Electron Transport Complex IV