A light and electron microscopic radioautographic study on RNA synthesis in the retina of chick embryo.
The incorporation of 3H-uridine into RNA of chick embryo retina was studied by light and electron microscopic radioautography. The numbers of silver grains were counted over the nucleus, nucleolus and cytoplasm of the cells in three different regions of the same retina of 2, 3, 4, and 7 day chick embryos. The results showed an increase of 3H-uridine incorporation from embryonic day 2 to 7. In every stage of development of the chick embryo retina, the number of silver grains was higher in the anterior than in the other two regions of the retina. In the three cell compartments of every embryo group, the number of silver grains was higher in the nucleus than in the nucleolus and cytoplasm. The results show further that the grains were less in the cytoplasm of the retinal cells of the day 2 embryo group and higher in the other groups especially in the day 7 embryos. Ultrastructural changes were also observed during the studied period of retina development.
Gunarso
W
W
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Gao
F
F
Cui
H
H
Ma
H
H
Nagata
T
T
D000818:Animals; D001345:Autoradiography; D002451:Cell Compartmentation; D002466:Cell Nucleolus / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002642:Chick Embryo; D003593:Cytoplasm / Q000737:chemistry; D008854:Microscopy, Electron; D012313:RNA / Q000096:biosynthesis*; D012160:Retina / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D012313:RNA; D014529:Uridine