Effect of combined lysozyme and lipase treatment on the survival of Listeria monocytogenes.
The effectiveness of polyphosphates or lipases to increase the lytic activity of lysozyme was evaluated both on Listeria monocytogenes suspended in buffer and on growing cultures incubated at different temperatures. At 5 degrees C and 37 degrees C polyphosphates combined with lysozyme did not result in the decrease of the number of non-growing L. monocytogenes cells. At the same incubation conditions, the addition of lipase to lysozyme significantly enhanced the bactericidal activity of lysozyme to an extent determined by pH, NaCl concentration and temperature.
Liberti
R
R
Food Department, Istituto Superiore di Sanitá, Rome, Italy.
Franciosa
G
G
Gianfranceschi
M
M
Aureli
P
P
D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D009113:Muramidase / Q000494:pharmacology*; D012965:Sodium Chloride; D013696:Temperature
D003470:Culture Media; D012965:Sodium Chloride; D008049:Lipase; D009113:Muramidase