Biochemical studies on the secretion of glycoproteins by isolated ciliary body of rabbits.
The contribution of the ciliary body to the origin of vitreous proteins was investigated in rabbits by incubating explants of this eye component under novel conditions. At the end of incubations for up to 21 h, the tissues were processed histologically and were shown to be in an excellent state of morphological preservation. When radioactive amino acids and fucose were added to the culture medium, protein and glycoprotein synthesis and secretion were detected using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) plus fluorography. The origin of these secretory products was traced by autoradiography to the ciliary epithelium. When samples of vitreous bodies - injected intravitreally with the same radioactive precursors - were run beside samples of the tissue culture media, comigration of at least 8 radioactively labelled bands including the one previously identified as transferrin was detected. This indicates that some vitreous proteins may be secreted by the ciliary body and that cultures of explants of ciliary body-iris are useful tools for studies on vitreous protein secretion.
Bertazolli Filho
R
R
Departamento de Morfologia, Faculdade de Medicina de Ribeirão Preto-USP, Brasil.
Laicine
E M
EM
Haddad
A
A
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D007498:Iris / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits
D000596:Amino Acids; D003470:Culture Media; D005136:Eye Proteins; D006023:Glycoproteins; D011500:Protein Synthesis Inhibitors; D005643:Fucose; D003513:Cycloheximide