Quantitative evaluation of passive cutaneous anaphylaxis (PCA) using a hand-held spectrophotometer.
A hand-held spectrophotometer was used for the quantitative evaluation of PCA caused by anti-TNP-IgE and TNP-BSA. A good relationship existed between the dilution of sera and the value of delta E*ab (a numerical expression of color) obtained by a hand-held spectrophotometer. In addition, the value of delta E*ab and the amount of Evans blue measured by the conventional colorimetric method correlated well. Because the method using a hand-held spectrophotometer provides a simple and objective analysis, it appears that the method is suitable as a substitute for the conventional method, which is time-consuming and requires killing animals cruelly.
Akiyama
H
H
National Institute of Health Sciences, Tokyo, Japan.
Teshima
R
R
Akasaka
R
R
Fujimori
K
K
Goda
Y
Y
Sawada
J
J
Toyoda
M
M
D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry