Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola.
Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recA [corrected] from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa, P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coli recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.
Aminov
R I
RI
STAFF-Institute, Ibaraki, Japan. aminov@gene.staff.or.jp
Nagamine
T
T
Ogata
K
K
Sugiura
M
M
Tajima
K
K
Benno
Y
Y
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D018720:Prevotella / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011938:Rec A Recombinases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D009153:Mutagens; D016685:Mitomycin; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases