A light- and electron-microscopic study of GluR4-positive cells in cerebral cortex, subcortical white matter and corpus callosum of neonatal, immature and adult rats.
The distribution of the [3H]alpha-amino-3-hydroxy-5-methylisoxzalepropionic acid (AMPA) receptor subunit GluR4 was studied in frontal, parietal and temporal cerebral cortex, subcortical white matter and corpus callosum of neonatal, immature and mature rats. In 1- to 2-day-old rats, a few oligodendrocyte progenitors and amoeboid microglia in the supraventricular part of the corpus callosum were immunolabelled for GluR4. At 7 to 10 days, the number of amoeboid microglia and oligodendrocyte progenitors in white matter increased; many neurons in cortex, including pyramidal neurons, were also moderately labelled for GluR4. The pattern of GluR4 immunostaining in 14-day-old rats was different from that in 7- to 10-day-old rats, but similar to the adult, in that there was no immunoreactivity in microglia and oligodendrocyte progenitors in subcortical white matter. A proportion of non-pyramidal neurons in cortex were moderately labelled, while some pyramidal neurons were lightly labelled. A population of small glial cells with features of oligodendrocyte progenitors were densely labelled in cortex.
Ong
W Y
WY
Department of Anatomy, National University of Singapore, Singapore. antongwy@leonis.nus.sg
Leong
S K
SK
Garey
L J
LJ
Reynolds
R
R
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002540:Cerebral Cortex / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D003337:Corpus Callosum / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis*
C088229:2-amino-4-phosphonobutyrate receptor; D000911:Antibodies, Monoclonal; D018094:Receptors, Metabotropic Glutamate