Modulating factors of individual sensitivity to diepoxybutane: sister chromatid exchanges induced in vitro in human lymphocytes.
Spontaneous and diepoxybutane (DEB)-induced sister chromatid exchanges (SCEs) were examined in cultured peripheral lymphocytes (PBL) from 122 healthy donors. SCE-inducing activity under defined experimental conditions and individual sensitivity to genotoxic stress were assessed. SCE means distribution appeared asymmetrical, identifying about 22% of subjects characterized by a 'high-respondent' phenotype with more than 111 SCEs/cell. Confounding factors, such as smoking habit, wine and coffee consumption, work activity and hematological factors, showed a limited capacity to affect individual SCE responsiveness, however hemoglobin and uric acid seemed to antagonize DEB genotoxicity.
Landi
S
S
Frenzilli
G
G
Sbrana
I
I
Barale
R
R
D000328:Adult; D000367:Age Factors; D000368:Aged; D000428:Alcohol Drinking; D002455:Cell Division; D002478:Cells, Cultured; D003069:Coffee; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology*; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000494:pharmacology*; D012044:Regression Analysis; D012737:Sex Factors; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012907:Smoking; D012959:Socioeconomic Factors; D014527:Uric Acid / Q000032:analysis
D003069:Coffee; D004852:Epoxy Compounds; D006454:Hemoglobins; D009153:Mutagens; D014527:Uric Acid; C007366:diepoxybutane