Loss of heterozygosity of microsatellite loci on chromosome 9p in astrocytic tumors and its prognostic implications.
We analyzed 19 samples of various astrocytic tumors (3 astrocytomas, 5 anaplastic astrocytomas, and 11 glioblastomas) for loss of heterozygosity (LOH) on chromosome 9p at 6 microsatellite loci (D9S54, IFNA, D9S171, D9S104, D9S165, and D9S166). Polymerase chain reaction was performed and the products were electrophoresed on polyacrylamide gel. As many as 16 of the 19 samples (84%) exhibited LOH. Three of the 7 informative loci (43%) showed LOH at D9S54, 7 of 17 (41%) at IFNA, 8 of 14 (57%) at D9S171, 7 of 14 (50%) at D9S104, 4 of 8 (50%) at D9S165, and 2 of 7 (29%) at D9S166. LOH was recognized in 57% of the informative loci in anaplastic astrocytomas and 54% in glioblastomas, while it was seen in only 8% of the astrocytomas. Accumulation of LOH with progression or recurrence of tumor was seen in 2 patients. Although, the survival period of the patients correlated well to the histological types of astrocytic tumors, we could not find any obvious correlations between the presence/absence of LOH and the survival period in these patients. In conclusion, we speculate that LOH on chromosome 9p is involved in malignant progression of astrocytomas, but has no significance in predicting survival period in these patients.
Maruno
M
M
Department of Neurosurgery, Osaka University Medical School, Japan.
Yoshimine
T
T
Muhammad
A K
AK
Tokiyoshi
K
K
Hayakawa
T
T
D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002648:Child; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics; D018450:Disease Progression; D005069:Evaluation Studies as Topic; D005260:Female; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006579:Heterozygote; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011379:Prognosis; D018709:Statistics, Nonparametric; D015996:Survival Rate
D004273:DNA, Neoplasm