title: Effects of a nitric oxide synthase inhibitor on prostaglandin-induced aqueous flare elevation in pigmented rabbits.
abstract: To evaluate the possible role of nitric oxide in the pathogenesis of exogenous prostaglandin (PG) induced aqueous flare elevation, we examined the effect of NG-nitro-L-arginine methyl ester (L-NAME), an L-arginine analogue acting as a specific inhibitor of nitric oxide synthase, on the elevation of aqueous flare in pigmented rabbits. L-NAME was injected into the ear vein of the animals. PGE1, PGE2, and PGF2 alpha were administered topically to the cornea using a glass cylinder. Aqueous flare was measured using a laser flare cell meter. PGE1, PGE2, and PGF2 alpha increased the aqueous flare dose dependently (5-100 micrograms/ml). L-NAME inhibited PGE1-, PGE2-, or PGF2 alpha-induced aqueous flare elevation. L-NAME, injected 30-90 min before PG application, inhibited the aqueous flare elevation maximally. We believe that nitric oxide may be involved in the pathogenesis of PG-induced aqueous flare elevation in rabbits.
authors.affiliation: Department of Ophthalmology, Medical and Pharmaceutical University, Toyama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hiraki
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Hayasaka
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D018916:Blood-Aqueous Barrier / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D003315:Cornea / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005136:Eye Proteins / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009883:Ophthalmic Solutions; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D004791:Enzyme Inhibitors; D005136:Eye Proteins; D009883:Ophthalmic Solutions; D011448:Prostaglandin Antagonists; D011453:Prostaglandins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester