Losses at 3p common deletion sites in subtypes of kidney tumours: histopathological correlations.
Deletions of the short arm of chromosome 3 (3p) have been recognized as characteristic features of clear cell renal cell carcinomas (clear cell RCC). We analysed 55 clear-cell RCCs and 30 non-clear-cell kidney tumours (10 papillary and 7 chromophobic RCCs, 11 oncocytomas and 2 collecting duct carcinomas) in loss of heterozygosity (LOH) studies using microsatellite markers for previously observed regions of common deletions on 3p in kidney tumours (3p25, 3p21.3, 3p14.2 and 3p12-13). Alterations were found in all 55 cases of clear-cell RCCs at two to four of the 3p regions. Extensive losses were not found in non-clear-cell tumours except for collecting duct carcinomas; 1 of 10 papillary RCCs showed interstitial deletion limited to a single 3p21.3 locus. LOH analyses using microsatellite markers for regions of common deletions at 3p may be of value in differential diagnosis of kidney tumours.
Hadaczek
P
P
Department of Genetics and Pathology, Pomeranian Medical Academy, Szczecin, Poland.
Podolski
J
J
Toloczko
A
A
Kurzawski
G
G
Sikorski
A
A
Rabbitts
P
P
Huebner
K
K
Lubinski
J
J
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000473:pathology; D000483:Alleles; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D002893:Chromosomes, Human, Pair 3; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D006579:Heterozygote; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004273:DNA, Neoplasm; D004276:DNA, Satellite