Analysis of diltiazem and desacetyldiltiazem in serum by high-performance liquid chromatography.
This paper describes a simple high-performance liquid chromatographic (HPLC) method for the determination of diltiazem and desacetyldiltiazem in human serum. After basic methyl-tert-butyl ether extraction and back-extraction with hydrochloric acid, the drug and its metabolite was injected into a Supelcosil LC-CN column and the absorbance of the eluate was measured at 240 nm. The sensitivity was 5 ng/ml and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with diltiazem.
Paczkowski
D
D
Department of Ischaemic Heart Disease, National Institute of Cardiology, Warszawa, Poland.
Dabrowski
R
R
Sitkiewicz
D
D
Sadowski
Z
Z
D002121:Calcium Channel Blockers / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans; D011786:Quality Control
D002121:Calcium Channel Blockers; C037769:deacetyldiltiazem; D004110:Diltiazem