CD69 expression on primitive progenitor cells and hematopoietic malignancies.
CD69 is an early activation antigen of peripheral blood lymphocytes and is constitutively expressed on a wide variety of bone marrow-derived cells. To further characterize the distribution and understand the potential biological role of the molecule in normal and malignant hematopoiesis, we used a novel high affinity anti-CD69 mAb (UN6) and analyzed hematopoietic progenitor cells together with a panel of myeloid and lymphoid malignancies. We report that mobilized peripheral blood CD34+ cells display detectable levels of CD69 and that the density of membrane expression correlates with the immature phenotype CD34bright Thy-1bright cells. Furthermore, during cytokine-induced differentiation, the expression of CD69 is moderately down-regulated. Analysis of hematopoietic malignancies revealed that CD69 expression correlates with the immature myeloid phenotype. Taken together these data suggest a role of CD69 during the early phase of hematopoiesis and in the leukemic transformation.
Tassone
P
P
Department of Experimental and Clinical Medicine, Facoltà di Medicina e Chirurgia, Catanzaro, Italy.
Turco
M C
MC
Tuccillo
F
F
Bonelli
P
P
Morrone
G
G
Cecco
L
L
Cerra
M
M
Bond
H
H
Di Nicola
M
M
Gianni
A M
AM
Venuta
S
S
D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D018952:Antigens, CD34 / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis*; D002648:Child; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D037181:Lectins, C-Type; D007938:Leukemia / Q000276:immunology*; D008214:Lymphocytes / Q000378:metabolism; D008875:Middle Aged; D063268:Sialic Acid Binding Ig-like Lectin 3; D018800:Thy-1 Antigens / Q000096:biosynthesis; D000099192:CD69 Antigens
D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D063268:Sialic Acid Binding Ig-like Lectin 3; D018800:Thy-1 Antigens; D000099192:CD69 Antigens; C571163:CD33 protein, human