title: On the difficulties of discriminating between major and minor hybrid male sterility factors in Drosophila by examining the segregation ratio of sterile and fertile sons in backcrossing experiments.
abstract: The observation of segregation ratios of sterile and fertile males in offspring samples from backcrossed hybrid females is, in principle, a valid method to unveil the genetic basis of hybrid male sterility in Drosophila. When the female parent is heterozygous (hybrid) for a sterility factor with major effects, equal proportions of fertile and sterile sons are expected in her offspring. However, intact (not recombined) chromosome segments of considerable length are expected to give segregation ratios that can not be easily differentiated from the 1:1 ratio expected from a single factor. When the phenotypic character under analysis can be determined by combinations of minor factors from the donor species spanning a certain chromosome length, very large offspring samples may be needed to test this alternative hypothesis against the null hypothesis of a single major factor. This is particularly the case of hybrid male sterility determinants in Drosophila.
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Spain.
authors.forename: X R
authors.initials: XR
authors.lastname: Maside
authors.forename: H F
authors.initials: HF
authors.lastname: Naveira
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000235:genetics; D017344:Genes, Insect; D006579:Heterozygote; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008957:Models, Genetic; D010641:Phenotype