ICAM-1 expression on keratinocytes in mechanically-injured skin of a patient with atopic dermatitis.
The expression of intercellular adhesion molecule-1 (ICAM-1) on keratinocytes was studied in skin specimens of patch- or scratch-test using Dermatophagoides farinae (DF) antigen in atopic dermatitis (AD). Positive patch test reaction showed exocytosis of lymphocytes and focal expression of ICAM-1 on keratinocytes. Similar lymphocyte infiltration and keratinocyte expression of ICAM-1 were observed in tape-stripped skin which was subjected as the control. In addition, focal ICAM-1 expression on keratinocytes was also observed without exocytosis of lymphocytes. By scratch-test with DF antigen, ICAM-1 expression on keratinocytes was observed in eight out of eleven patients. One specimen showed ICAM-1 expression in spite of the absence of exocytosis of lymphocytes. Two out of three specimens from negative scratch-test sites with control liquid (mixture of equal amount of saline and glycerine) showed ICAM-1 expression in a similar manner to that of the positive scratch tests. In normal control skin, patch-test sites showed focal and weak expression of ICAM-1. Normal appearing skin of AD also showed no ICAM-1 expression. Therefore, in patients with atopic dermatitis, keratinocytes may express ICAM-1 prior to infiltration of lymphocytes into the epidermis and this expression might be induced not only by cytokines produced by lymphocytes but also by mechanically injured (tape-stripped or scratched) epidermal cells.
Matsunaga
T
T
Department of Dermatology, Tokyo Medical and Dental University, Japan.
Katayama
I
I
Yokozeki
H
H
Nishioka
K
K
D000328:Adult; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D039741:Antigens, Dermatophagoides; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008925:Mites / Q000276:immunology; D010812:Physical Stimulation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000473:pathology; D012882:Skin Tests
D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1