title: Goodpasture syndrome: selective removal of anti-alpha 3 (IV) collagen autoantibodies. A potential therapeutic alternative to plasmapheresis.
abstract: Anti-alpha 3(IV) collagen autoantibodies have been implicated in the pathogenesis of Goodpasture syndrome, an autoimmune disorder causing glomerulonephritis and pulmonary hemorrhage. Currently treatment involves removal of the entire IgG fraction of plasma by plasmapheresis or adsorption to protein A. The present study shows that the anti-alpha 3(IV)NC1 autoantibodies can be removed from plasma specifically and quantitatively by affinity chromatography utilizing either alpha 3 NC1 domain of bovine type IV collagen or recombinant alpha 3 NC1 domain of human type IV collagen immobilized to agarose beads. This study shows the feasibility of using affinity chromatography, as an alternative to plasmapheresis, to exclusively remove the pathogenic autoantibodies from the plasma of patients with Goodpasture syndrome.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Boutaud
authors.forename: R
authors.initials: R
authors.lastname: Kalluri
authors.forename: T Z
authors.initials: TZ
authors.lastname: Kahsai
authors.forename: M E
authors.initials: ME
authors.lastname: Noelken
authors.forename: B G
authors.initials: BG
authors.lastname: Hudson
mesh_terms: D000327:Adsorption; D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D001323:Autoantibodies / Q000097:blood* / Q000302:isolation & purification; D015153:Blotting, Western; D002417:Cattle; D002846:Chromatography, Affinity; D003094:Collagen / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D010956:Plasmapheresis; D011994:Recombinant Proteins / Q000276:immunology; D013205:Staphylococcal Protein A
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D003094:Collagen