In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method.
The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.
Ciapetti
G
G
Laboratorio di Biocompatibilità dei Materiali da Impianto, Istitutì Ortopedici Rizzoli, Bologna, Italy.
Verri
E
E
Granchi
D
D
Cenni
E
E
Gamberini
S
S
Benetti
D
D
Mian
M
M
Pizzoferrato
A
A
D000704:Analysis of Variance; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism* / Q000494:pharmacology; D004396:Coloring Agents; D006651:Histocytochemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010587:Phagocytosis / Q000502:physiology*; D010851:Picrates / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D015503:Surgical Sponges; D014945:Wound Healing / Q000187:drug effects
D001391:Azo Compounds; D004396:Coloring Agents; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D010851:Picrates; C009798:C.I. direct red 80; D003094:Collagen; C005858:picric acid