A multiwell plate procedure for immunohistochemical and histochemical staining of constituents of articular cartilage.
A multiwell plate procedure was tested for its applicability to determine immunohistochemically the noncollagenous matrix protein COMP (cartilage oligomeric matrix protein) and histochemically the proteoglycans of the matrix of articular cartilage. Fixed and decalcified cartilage-bone sections were treated with buffer, antisera, substrate or staining solutions in the wells of an assay plate under shaking on a rocking table. This floating procedure results in a reproducible histochemical or immunohistochemical staining and might therefore be valuable to determine or to detect other constituents of the matrix of these connective tissues under comparable conditions.
Müller
G
G
Institute of Hygiene and Environmental Medicine, University of Greifswald, Germany.
Altenburg
E
E
D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry*; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D064235:Matrilin Proteins; D014048:Tolonium Chloride
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D014048:Tolonium Chloride