title: Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.
abstract: Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.
authors.affiliation: Department of Medicine, Sahlgrenska University Hospital, University of Göteborg, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Stockelberg
authors.forename: M
authors.initials: M
authors.lastname: Hou
authors.forename: L
authors.initials: L
authors.lastname: Rydberg
authors.forename: J
authors.initials: J
authors.lastname: Kutti
authors.forename: H
authors.initials: H
authors.lastname: Wadenvik
mesh_terms: D000017:ABO Blood-Group System / Q000032:analysis*; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016824:Antigens, Human Platelet / Q000737:chemistry* / Q000276:immunology; D001792:Blood Platelets / Q000737:chemistry*; D018955:CD36 Antigens / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006031:Glycosylation; D006801:Humans; D007231:Infant, Newborn; D039661:Integrin beta3; D051379:Mice; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000737:chemistry* / Q000276:immunology; D011499:Protein Processing, Post-Translational; D013921:Thrombocytopenia / Q000151:congenital / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D018955:CD36 Antigens; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019038:Platelet Glycoprotein GPIb-IX Complex