Secretion of type-1-fimbriae binding proteins from human neutrophil granulocytes.
Granule matrix proteins secreted from human neutrophils after ionomycin stimulation were separated by SDS-PAGE, blotted onto a polyvinylidene diflouride (PVDF) membrane and overlaid with the mannose-binding lectin concanavalin A (Con A) or Escherichia coli bacteria exposing type-I-fimbriae. Four proteins of approximately 30, 40, 70 and 80 kD, respectively, derived from both the azurophil and the specific granules were shown to expose mannose-containing structures by binding of Con A. Such reactivity was also shown for a 90-kD protein from the light membrane fraction enriched in plasma membrane and secretory vesicles. When blots of granule matrix proteins were exposed to type-I-fimbriated bacteria, a total of seven proteins was recognized; four of the five Con A-binding proteins (40, 70, 80 and 90 kD) was detected also by the bacteria in addition to three proteins not detected by Con A (50, 60 and 100 kD). The role of the secreted type-1-fimbriae binding proteins as anti-adhesin candidates is discussed.
Karlsson
A
A
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Dahlgren
C
C
D000328:Adult; D001422:Bacterial Adhesion; D002118:Calcium / Q000502:physiology; D003208:Concanavalin A / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000737:chemistry; D003599:Cytoskeleton / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D010587:Phagocytosis
D006023:Glycoproteins; D007476:Ionophores; D008562:Membrane Glycoproteins; D003208:Concanavalin A; D003571:Cytochalasin B; D015759:Ionomycin; D008358:Mannose; D002118:Calcium