Lack of transforming activity of fumonisin B1 in BALB/3T3 A31-1-1 mouse embryo cells.
The capacity of fumonisin B1 (FB1) to induce morphological transformation of cultured mammalian cells was assessed using BALB/3T3 A31-1-1 mouse embryo cells. FB1 with 90% purity was prepared from Fusarium proliferatum grown on whole corn. Cell growth was not inhibited by 48 hr of exposure at concentrations up to 1000 micrograms/ml. Moderate inhibition was induced by 6 days of exposure. In transformation assays with a 48-hr exposure, increases in transformed foci were observed at some concentrations; however, the responses were not reproducible. Prolonged exposure for up to 4 wk at 10, 100 and 500 micrograms/ml failed to induce increases in transformed foci. Analysis of combined results showed that only the increase induced by a 48-hr exposure at 500 micrograms/ml was significant. A trend test indicated the lack of a dose response for concentrations of 10-1000 micrograms/ml. FB1 seems to lack in vitro transforming activity.
Sheu
C W
CW
Genetic Toxicology Branch, Food and Drug Administration, Washington DC 20204, USA.
Rodriguez
I
I
Eppley
R M
RM
Lee
J K
JK
D000818:Animals; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D037341:Fumonisins; D005670:Fusarium / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D009183:Mycotoxins / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D013723:Teratogens / Q000633:toxicity*; D003313:Zea mays / Q000382:microbiology
D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; D013723:Teratogens; C056933:fumonisin B1