title: Identification of a cAMP-dependent protein-DNA interaction at a sequence near the yeast mitochondrial rRNA genes.
abstract: We have shown that expression of yeast mitochondrial (mt) rRNA genes (S. cerevisiae) is controlled in a cAMP-dependent manner via PKA, suggesting a trans-activation process involving phosphorylation-dependent protein-mt DNA interaction. We used filter-binding assays, mt protein extracts, and mt DNA from a rho-mutant strain retaining the 21S rRNA gene to demonstrate such an interaction. Competition assays with the cloned 21S-related mt DNA fragment undergoing interaction showed that a sequence in that fragment is present in mt DNA from a rho-strain retaining the 16S mt rRNA gene, but not in a VAR1-retaining rho-strain that lacks cAMP-mediated mt transcription. The sequence of the 21S-related mt DNA fragment undergoing protein interaction includes a GC cluster; that GC cluster sequence is also present near the 16S gene but not near VAR1. These and other data are consistent with a role for the GC cluster in cAMP-mediated expression of mt rRNA genes.
authors.affiliation: Dept. Microbiology and Immunology, Medical College of Pennsylvania-Hahnemann University, PA 19104, USA.
authors.forename: M U
authors.initials: MU
authors.lastname: Rahman
authors.forename: J
authors.initials: J
authors.lastname: Iqbal
authors.forename: W A
authors.initials: WA
authors.lastname: Salameh
authors.forename: C A
authors.initials: CA
authors.lastname: Nevel-McGarvey
authors.forename: A P
authors.initials: AP
authors.lastname: Hudson
mesh_terms: D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D012335:RNA, Ribosomal; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases