Use of continuous human lung cell culture for adenovirus isolation.
Adenovirus sensitivity, TCD50 value and mean time to cytopathic effect (CPE) were compared in Madin-Darby canine kidney (MDCK), rhesus monkey kidney (RhMK), human lung carcinoma (A549) and in newly identified continuous human lung (CHL) cell cultures. High-titer stock and positive original specimens were inoculated in culture. Patients specimens were also placed in shell-vial cultures. A549 and CHL cells demonstrated comparable sensitivity, however the former generally produced CPE earlier. Sensitivity was respectively reduced and poor in RhMK and MDCK cultures, as expected in cells of nonhuman origin. The performance of CHL for adenoviruses and other pathogens makes them a useful addition in diagnostic virology laboratories.
Leonardi
G P
GP
Department of Pathology, Nassau County Medical Center, East Meadow, N.Y. 11554, USA.
Costello
P
P
Harris
P
P
D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D006801:Humans; D008168:Lung / Q000821:virology*; D012680:Sensitivity and Specificity