Techniques for investigation of an apparent outbreak of infections with Candida glabrata.
A cluster of Candida glabrata isolates recovered from seven patients in an intensive care unit over a 10-week period were compared with a collection of isolates from six epidemiologically distinct outpatients and a reference strain by several DNA typing methods. Restriction enzyme analysis with HinII distinguished 13 strains from the 14 sources and was the method of choice. Pulsed-field gel electrophoresis and random amplification of polymorphic DNA both detected nine types from the 14 sources; however, the results of these two methods did not always correlate. These methods demonstrated that five of the seven patients had distinguishable strains and that cross-infection was unlikely.
Arif
S
S
Department of Microbial Diseases, St. John's Institute of Dermatology, London, United Kingdom.
Barkham
T
T
Power
E G
EG
Howell
S A
SA
D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology*; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D004196:Disease Outbreaks; D006801:Humans; D007362:Intensive Care Units; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004271:DNA, Fungal