Lytic activity of IL-2 and IL-12 stimulated NK cells against HOS osteosarcoma cell line.
NK cells are CD16, CD56 positive lymphocytes that spontaneously lyse tumor or virus infected cells. In this study we investigated whether IL-2 and/or IL-12 stimulated NK cells increased their lytic efficiency against HOS osteosarcoma cell line. Our results demonstrate that both 18 hour and 5 day incubation times enhanced the lytic activity of human PBL against HOS and K562 target cells and that IL-12 appears to be more efficient than IL-2 in augmenting NK cytotoxicity.
Mariani
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Laboratorio di Immunologia e Genetica, Università di Bologna.
Tarozzi
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Meneghetti
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Tadolini
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Facchini
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D000328:Adult; D001859:Bone Neoplasms / Q000473:pathology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D018664:Interleukin-12