Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography.
Amphoteric poly(Lys-[Glu1.0-DL-Ala4.1]), (EAK) and anionic poly(Lys-Ac-Glu0.98-DL-Ala3.98]), (AcEAK) branched chain polypeptides were analyzed by capillary electrophoresis (CE) and micellar elektrokinetic chromatography (MEKC) in the following buffers. A1: 0.25 N triethyl ammonium phosphate (TEAP) buffer (pH 2.25); A2: 100 mM sodium dodecyl sulfate (SDS) in buffer A1; B1: Na-borate buffer (pH 7.7); B2: 100 mM SDS in buffer B1; C1: Na-borate buffer (pH 11.0); C2: 100 mM SDS in buffer C1. Both EAK and AcEAK could be separated by a CE mechanism at pH 2.25 and by an MEKC mechanism at pH 11.0. Optimum results were achieved with CE in buffer A1 and with MEKC in buffer C2.
Idei
M
M
Semmelweis University of Medicine, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary. miki@puskin.sote.hu
Dibó
G
G
Bogdán
K
K
Mezö
G
G
Horváth
A
A
Erchegyi
J
J
Mészáros
G
G
Teplán
I
I
Kéri
G
G
Hudecz
F
F
D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010455:Peptides / Q000032:analysis*
D002021:Buffers; D008823:Micelles; D010455:Peptides