title: Polysomies of chromosomes 7 and 17 in head and neck squamous cell carcinomas.
abstract: OBJECTIVE
  To determine the polysomies (ie, cells with > or = 3 chromosome copies) of chromosomes 7 and 17 in tumor tissue, histologically normal epithelia adjacent to the tumor (tumor-adjacent epithelia), and buccal epithelia distant from the tumor (tumor-distant epithelia) of head and neck squamous cell carcinomas.
  
  
  METHODS
  Nonfluorescent, nonisotopic, in situ hybridization using chromosome-specific centromeric DNA probes for chromosomes 7 and 17 was performed on the formalin-fixed, paraffin-embedded specimens from tumor and tumor-adjacent and tumor-distant epithelia of 19 patients with head and neck squamous cell carcinomas and from buccal epithelia of cancer-free control subjects who smoked and did not smoke cigarettes.
  
  
  METHODS
  Four hundred nuclei in tumor tissue and 200 nuclei in histologically normal epithelia were scored for hybridized signals in each sample.
  
  
  RESULTS
  Buccal epithelia of cancer-free control subjects who smoked and did not smoke cigarettes showed no difference in the polysomies of chromosomes 7 and 17, respectively. The polysomies of chromosomes 7 and 17 in the tumor cells were higher than those in the tumor-adjacent epithelia (P < .001 for both), tumor-distant epithelia (P < .001 for both), and buccal epithelia of cancer-free control subjects who smoked and did not smoke cigarettes (P < .001 for both). The polysomies of chromosomes 7 and 17 in the tumor-adjacent epithelia were higher than those in the tumor-distant epithelia (P < .001 for both) and the control buccal epithelia (P = .002 and P < .001, respectively). The tumor-distant epithelia and the control buccal epithelia for the polysomies of chromosomes 7 and 17 had no differences.
  
  
  CONCLUSIONS
  The finding of genotypic abnormalities in the tumor-adjacent epithelia supports the concept of field cancerization. Such genotypic parameters may provide a genetic basis for the development of an early recurrence or second primary tumors after therapeutic treatment of head and neck squamous cell carcinomas.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul.
authors.forename: G
authors.initials: G
authors.lastname: Choi
authors.forename: K
authors.initials: K
authors.lastname: Chung
mesh_terms: D000368:Aged; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D015342:DNA Probes; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000648:ultrastructure; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D010610:Pharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D012907:Smoking
chemical_list: D015342:DNA Probes