Diagnostic molecular pathology in the twenty-first century.
Diagnostic molecular pathology is expanding rapidly with the aid of the Human Genome Project and the development of potentially user-friendly molecular diagnostic methods. The diagnostic molecular pathology laboratory of the future must be prepared to purify DNA or RNA from a variety of sources and to investigate the sequence of the target genome of interest using automated amplification and hybridization detection systems. There will be a shift in the emphasis from phenotypic to genotypic diagnosis, and the diagnostic molecular pathology laboratory of the early twenty-first century will perform 5% to 10% of the volume of all laboratory testing.
Kiechle
F L
FL
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan, 48073, USA.
D001331:Automation; D002256:Carbonic Anhydrases / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D005544:Forecasting; D005784:Gene Amplification; D005821:Genetic Techniques / Q000639:trends*; D005820:Genetic Testing / Q000592:standards / Q000639:trends*; D015316:Genetic Therapy / Q000592:standards; D006801:Humans; D009693:Nucleic Acid Hybridization; D010338:Pathology, Clinical / Q000639:trends*; D012313:RNA / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014481:United States
D012313:RNA; D004247:DNA; D002256:Carbonic Anhydrases