Use of polymorphic short and clustered coding-region microsatellites to distinguish strains of Candida albicans.
We describe the identification of polymorphic microsatellite loci in the pathogenic yeast, Candida albicans. A search for all coding-region microsatellites with more than four repeats that can be found in Candida sequences in GenBank was conducted. Nine such microsatellite sequences consisting of trinucleotide motifs were found. Three of these were perfect microsatellites while the remaining six sequences were found in one imperfect microsatellite and two compound microsatellites. Because of the close proximity of some of these repeats, all could be assayed with six PCR primer pairs. All of these microsatellite sequences were found in five nuclear genes, ZNF1, CCN1, CPH1, EFG1, and MNT2. Except for a single (CTT)5 serine tract, all coded for polyglutamine tracts. Another locus with seven alleles, a region of the ERK1 protein kinase gene, was also examined, and may be a representative of a new class of highly polymorphic "clustered' microsatellites. Such loci, in which several non-contiguous but closely linked microsatellites are clustered together, may be a useful source of DNA polymorphisms in microorganisms in which long microsatellite sequences are unavailable. All seven regions amplified were polymorphic, having between two and seven variable length alleles in the 11 strains of Candida albicans examined. The results of this and similar searches will facilitate epidemiological and evolutionary studies of Candida and other microorganisms.
Field
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D
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
Eggert
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L
Metzgar
D
D
Rose
R
R
Wills
C
C
D000483:Alleles; D005075:Biological Evolution; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D002176:Candida albicans / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D018895:Microsatellite Repeats; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004271:DNA, Fungal; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases