Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine.
An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5c,10;P1.12,13;P1.7h,4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes.
Claassen
I
I
Laboratory for the Control of Biological Products, National Institute for Public Health and Environmental Protection, Bilthoven, The Netherlands.
Meylis
J
J
van der Ley
P
P
Peeters
C
C
Brons
H
H
Robert
J
J
Borsboom
D
D
van der Ark
A
A
van Straaten
I
I
Roholl
P
P
Kuipers
B
B
Poolman
J
J
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D006801:Humans; D008585:Meningitis, Meningococcal / Q000517:prevention & control; D051379:Mice; D008854:Microscopy, Electron; D009345:Neisseria meningitidis / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines