title: Metabolic and histologic effects of recombinant canine somatotropin on bone healing in dogs, using an unstable ostectomy gap model.
abstract: OBJECTIVE
  To investigate the effect of recombinant canine somatotropin (STH) on the metabolic and histologic aspects of bone healing in dogs, using an unstable ostectomy gap model.
  
  
  METHODS
  8 mature dogs.
  
  
  METHODS
  A 3-mm ostectomy of the mid portion of the radius was performed in all dogs. Implants designed to release STH at a rate of 4 mg/d were placed SC in 4 dogs (treated group [STHG]), and another 4 dogs received no implants (control group [CG]). Serum concentrations of STH, insulin-like growth factor I, and osteocalcin were determined before surgery, and weekly for 8 weeks. Scintigraphic evaluation of the ostectomy sites was performed before surgery, and at weeks 2, 4, 6, and 8 after surgery. Histologic evaluation ofthe ostectomy sites was performed at the conclusion of the study at week 8.
  
  
  RESULTS
  Significant (P < 0.05) increases in serum STH, insulin-like growth factor I, and osteocalcin concentrations were observed in dogs of the STHG during the 8-week study period. Scintigraphic activity of the ostectomy sites was increased in dogs of both groups, but dogs of the STHG had significantly (P < 0.05) greater activity, compared with dogs of the CG. Coalescence of nuclear activity across the ostectomy site was observed in dogs of the STHG, whereas dogs of the CG maintained 2 distinct areas of metabolic activity. Histologically, dogs of the STHG had bridging calluses with areas of endochondral ossification and ongoing osteogenic activity, whereas dogs of the CG had nonossified fibrocartilage typical of nonunion fractures.
  
  
  CONCLUSIONS
  Using the ostectomy gap model, recombinant canine STH enhanced the metabolic and histologic aspects of bone healing in dogs.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Wilkens
authors.forename: D L
authors.initials: DL
authors.lastname: Millis
authors.forename: G B
authors.initials: GB
authors.lastname: Daniel
authors.forename: L
authors.initials: L
authors.lastname: Munson
authors.forename: K R
authors.initials: KR
authors.lastname: Patel
authors.forename: F C
authors.initials: FC
authors.lastname: Buonomo
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D017102:Fracture Healing / Q000187:drug effects*; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D015675:Osteocalcin / Q000097:blood; D011884:Radius / Q000473:pathology / Q000601:surgery; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone