Dacron stimulation of macrophage transforming growth factor-beta release.
This study evaluated the effect of Dacron on the release of macrophage transforming growth factor-beta (TGF-beta),an endothelial cell growth inhibitor. Rabbit peritoneal macrophages were grown in minimum essential medium (MEM) with 10% fetal bovine serum (FBS) in the presence or absence of Dacron (0.5 mm x 3 mm particles). Media were collected three times each week for 7 weeks. For the TGF-beta bioassay, mink lung epithelial cells (CCL64) were grown in MEM with 10% FBS. Test-conditioned media, 100 mu 1, were added (n = 4), and incubated 48 h. 3(H)-Thymidine (3(H)-TdR) uptake was determined and compared with 3(H)-TdR uptake using known pure TGF-beta standards. Media samples were additionally pre-incubated with a neutralizing anti-TGF-beta(1) antibody and the 3(H)-TdR uptake again quantitated. TGF-beta activity in the conditioned media of macrophages exposed to Dacron exceeded the control media groups in all weeks, reaching significance (P<0.05) in weeks 3, 4,5, 6 and 7. Pre-incubation of media samples with the anti-TGF-beta antibody inhibited this TGF-beta activity in all weeks with statistical significance in weeks 1, 2, 3, 5 and 7. The inhibitory effects of Dacron on endothelialization may be explained by the Dacron-induced release of TGF-beta from macrophages.
Greisler
H P
HP
Loyola University Medical Center, Department of Surgery, Maywood, Illinois, USA.
Petsikas
D
D
Cziperle
D J
DJ
Murchan
P M
PM
Henderson
S C
SC
Lam
T M
TM
D000818:Animals; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism*; D008907:Mink; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D017077:Culture Media, Conditioned; D011093:Polyethylene Terephthalates; D016212:Transforming Growth Factor beta