Sodium lauryl sulfate effect on the density of epidermal Langerhans cells. Evaluation of different test models.
The effect of different test models for sodium lauryl sulfate (SLS)-induced irritant contact dermatitis on epidermal Langerhans cells (LC) numbers was examined. Finn Chambers, 8 and 12 mm, containing 15 and 34 or 50 microliters, respectively, of 1% aq. solution of SLS were applied to human forearm skin for 48 h as single or repeated application. The results showed a clear difference between the effects with the 2 chamber sizes. The effect of the 8-mm chambers could result in increased, unchanged or decreased LC numbers, while 12-mm chambers always produced a decrease. These results seem to explain, at least partly, the discrepant results reported from various laboratories.
Mikulowska
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Dept. of Medical Cell Research, University of Lund, Sweden.
Andersson
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D000328:Adult; D001706:Biopsy; D002452:Cell Count / Q000187:drug effects; D004396:Coloring Agents; D017453:Dermatitis, Irritant / Q000175:diagnosis; D015194:Diffusion Chambers, Culture; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects*; D005069:Evaluation Studies as Topic; D005542:Forearm; D006801:Humans; D007150:Immunohistochemistry; D007509:Irritants / Q000008:administration & dosage / Q000494:pharmacology*; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D012867:Skin; D012882:Skin Tests / Q000295:instrumentation / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D004396:Coloring Agents; D007509:Irritants; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate