The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence.
An experimental system which assesses the antioxidant potential of ascorbic acid, glutathione, uric acid, and taurine was developed. The system comprised hemoglobin, luminol, t-butyl hydroperoxide, and different concentrations of antioxidants in TRIS-HCl buffer (pH 7.4). Control assays were performed by excluding antioxidants. Chemiluminescence was detected using a liquid scintillation counter in single photon mode. All antioxidants, when applied in the appropriate concentrations, decreased the maximum chemiluminescence values. The minimum concentrations which decreased the chemiluminescence values were defined for each of the antioxidants.
Gümüşlü
S
S
Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey.
Yücel
G
G
Aydin
M
M
Yeşilkaya
A
A
Demir
A Y
AY
Aksu
T A
TA
D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000502:physiology; D005978:Glutathione / Q000502:physiology; D008163:Luminescent Measurements; D010545:Peroxides / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species; D013654:Taurine / Q000502:physiology; D014527:Uric Acid / Q000378:metabolism; D020122:tert-Butylhydroperoxide
D000975:Antioxidants; D010545:Peroxides; D017382:Reactive Oxygen Species; D013654:Taurine; D014527:Uric Acid; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D001205:Ascorbic Acid