Effects of glycerol of beta-lactamase production during high cell density cultivation of recombinant Escherichia coli.
Recombinant Escherichia coli was cultured up to OD600 90 with glycerol fed by the DO-STAT method to produce recombinant beta-lactamase. Optimum feeding and induction conditions were determined. When glycerol was maintained at a high concentration (40-50 g/L) after isopropyl thiogalactoside (IPTG) induction at OD600 75, a 2-fold increase in the amount of soluble beta-lactamase was obtained. Similarly, a 3-fold increase was obtained by the addition of NaCl (0.4 M) at low glycerol concentration (10 g/L).
Kwon
S
S
Department of Biological Engineering, Inha University, Inchon, Namgu, Korea.
Kim
S
S
Kim
E
E
D004926:Escherichia coli / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D001618:beta-Lactamases / Q000096:biosynthesis*
D011994:Recombinant Proteins; D001618:beta-Lactamases; D005990:Glycerol