Fluorescence emission and anisotropy from rhodamine dimers.
The absorption, fluorescence emission, and excitation fluorescence anisotropy spectra of a rhodamine dye in a water-glycerol solution at high concentration were investigated to determine spectroscopic properties of the ground state dimer. The combination of data from these spectra measured at several dye concentrations contained sufficient constraints on the model for dimer association to permit an estimate of: the association constant, the extinction coefficients, the relative fluorescence quantum yield, and the emission spectra of the monomeric and dimeric species. The rhodamine dimer is an efficient fluorescence emitter with fluorescence anisotropy equivalent to that of the pure monomeric species over the range of excitation wavelengths covering its two lowest energy transitions.
Burghardt
T P
TP
Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN 55905, USA. burghardt@mayo.edu
Lyke
J E
JE
Ajtai
K
K
D016880:Anisotropy; D004563:Electrochemistry; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence
D012235:Rhodamines; D012996:Solutions