title: Ca(2+)-induced Ca2+ release activates K+ currents by a cyclic GMP-dependent mechanism in single gastric smooth muscle cells.
abstract: The participation of sarcoplasmic reticulum Ca2+ release channels in the activation of Ca(2+)-sensitive K+ currents (IK(Ca)) by cyclic dibutyryl GMP was investigated in smooth muscle cells from the circular layer of guinea-pig gastric fundus. All experiments were performed in the presence of 3 microM nicardipine into the bath and low Ca2+ buffering capacity of the pipette-filling solution (pCa 7.4). Ruthenium red (10 microM) as well as its combination with 10 microM heparin abolished the cyclic GMP-induced activation of IK(Ca), while 10 microM heparin remained ineffective. Ryanodine (10 microM) and the subsequently added 1 microM thapsigargin induced a relatively small increase in IK(Ca) amplitudes. The addition of 10 microM ryanodine to 1 microM thapsigargin-containing bath solution caused a vast increase in IK(Ca). It is hypothesyzed that protein kinase G-induced vectorial Ca2+ flux from the cell bulk and sarcoplasmic reticulum Ca2+ stores toward the plasma membrane is realized by a spontaneous Ca(2+)-induced Ca2+ release from a superficially situated Ca2+ store.
authors.affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia. DESSYBD@IPH.BIO.ACAD.BG
authors.forename: D B
authors.initials: DB
authors.lastname: Duridanova
authors.forename: H S
authors.initials: HS
authors.lastname: Gagov
authors.forename: K K
authors.initials: KK
authors.lastname: Boev
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006152:Cyclic GMP / Q000494:pharmacology*; D006168:Guinea Pigs; D009130:Muscle, Smooth / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects*; D012433:Ryanodine / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects*
chemical_list: D015221:Potassium Channels; D012433:Ryanodine; D006152:Cyclic GMP; D002118:Calcium