Unilateral renal ischemia reperfusion in the rat: effect of blood volume trapped in the kidney, sucrose infusion, and antioxidant treatments.
This study was carried out in order to examine whether the severity of acute renal failure observed during the four hours following a 45 min period of unilateral occlusion of the renal pedicle could be reduced by various treatments. These include intrarenal flush with saline immediately before the occlusion, by sucrose infusion immediately before reperfusion, or by injection of NAO (natural antioxidant) and vitamin E before the occlusion. After renal pedicle occlusion, creatinine levels increased to 165% of their pre-ischemic values. Urine flow, GFR, renal cortex blood flow and NADH decreased by 99%, 99%, 50% and 36%, respectively. A decrease in the Na and K reabsorption (15% and 32%, respectively) was also observed. Partial protection of renal function against ischemic damage was observed when kidney tissue remained blood-free, by exposing it to saline throughout the period of ischemia. Significant protection was observed after treatment with sucrose, vitamin E and NAO. This study demonstrates that it is possible to attenuate the injury to the ischemic kidney by inducing ischemia in a bloodless kidney, by inducing diuresis in the first phase of reperfusion, or by antioxidant treatment, such as vitamin E or NAO.
Zurovsky
Y
Y
Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
Eligal
Z
Z
Grossman
S
S
D058186:Acute Kidney Injury / Q000517:prevention & control; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001810:Blood Volume / Q000187:drug effects*; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology*; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000009:adverse effects*; D015427:Reperfusion Injury / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology*; D013395:Sucrose / Q000627:therapeutic use*; D014810:Vitamin E / Q000627:therapeutic use
D000975:Antioxidants; D010936:Plant Extracts; D014810:Vitamin E; D013395:Sucrose