Solid-phase extraction and HPLC analysis of kebuzone and its metabolites in blood.
A HPLC method for quantification of kebuzone and its metabolites in whole blood was developed. The compounds and the internal standard were isolated from blood by solid-phase extraction on a C-18 cartridge. A blood sample was to be hemolyzed before extraction. HPLC was performed on a C-18 column with the mobile phase composed of methanol/water acidified to pH 2.7 and UV absorbance detection at 247 nm. This method has been successfully applied to a pharmacokinetic study of kebuzone and its metabolites in rabbits.
Klimes
J
J
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Repulic.
Sochor
J
J
Sedlacek
J
J
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002492:Central Nervous System Depressants / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D008702:Methaqualone / Q000097:blood / Q000493:pharmacokinetics; D010653:Phenylbutazone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002492:Central Nervous System Depressants; C100280:kebuzone; D008702:Methaqualone; D010653:Phenylbutazone