Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45.
We studied the effect of incubating murine lymphocytes with cis-unsaturated fatty acids on expression and capping of CD44 and CD45. Lymphocytes were incubated with stearic (18:0) or oleic (18:1 omega-9) acid bound to bovine serum albumin (BSA). After incubation with rat anti-CD44 or anti-CD45 monoclonal antibodies and then with fluorescent-labeled anti-rat antibody, mean fluorescence intensity (FI) was measured by using flow cytometry. Capping was measured after warning and fixation in paraformaldehyde. Steady-state fluorescence anisotropy (rs) was measured after the cells had been incubated with trimethylammoniumdiphenylhexatriene. Incubation with oleic acid, but not stearic acid or BSA alone, was associated with an increase in FI of CD44. Expression of CD45, however, was increased by both stearic and oleic acids to the same degree over BSA controls. CD44 and CD45 capping were both increased by incubation with oleic acid. Rs was decreased in cells incubated with oleic acid, suggesting an increase in membrane fluidity. We conclude that incubation with oleic acid increases expression of CD44 and increases capping of both CD44 and CD45. These findings were confirmed in feeding experiments, in which rs was reduced and CD44 capping increased by polyunsaturated fatty acid diets.
Peck
M D
MD
Department of Surgery, University of Miami School of Medicine, Florida, USA.
Li
Z
Z
Han
T
T
Wang
W
W
Jy
W
W
Ahn
Y S
YS
Ziboh
V A
VA
Chu
A J
AJ
Bourguignon
L Y
LY
D000818:Animals; D000911:Antibodies, Monoclonal; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D018960:Hyaluronan Receptors / Q000032:analysis* / Q000276:immunology; D007152:Immunologic Capping / Q000187:drug effects*; D017493:Leukocyte Common Antigens / Q000032:analysis* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid / Q000494:pharmacology; D012710:Serum Albumin, Bovine; D013229:Stearic Acids / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D018960:Hyaluronan Receptors; D013229:Stearic Acids; D012710:Serum Albumin, Bovine; D019301:Oleic Acid; C031183:stearic acid; D017493:Leukocyte Common Antigens