Mediator of transcriptional regulation.
A multi-protein complex, termed mediator, has been isolated from yeast, based on its requirement for transcriptional activation in a system reconstituted from pure RNA polymerase II and general transcription factors. Mediator polypeptides include the products of many genes previously recovered from screens for mutations affecting transcription. This connection between biochemical and genetic studies reveals that mediator is important for both activation and repression of transcription, and that mediator plays a role in transcriptional regulation in vivo as well as in vitro. Mediator binds the carboxy-terminal domain of RNA polymerase II, forming a polymerase holoenzyme, whose possible association with additional proteins is a subject of some controversy.
Björklund
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Department of Medical Biochemistry and Biophysics, Umeå University, Sweden. Stefan.Bjorklund@medchem.umu.se
Kim
Y J
YJ
D001665:Binding Sites; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D056892:Mediator Complex; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics
D005656:Fungal Proteins; C058337:GAL11 protein, S cerevisiae; D056892:Mediator Complex; C095061:RGR1 protein, S cerevisiae; D012097:Repressor Proteins; C076878:SIN4 protein, S cerevisiae; C093965:SRB7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012319:RNA Polymerase II