title: Rejoining of DNA double-strand breaks induced by accelerated nitrogen ions.
abstract: Rejoining of radiation-induced DNA double-strand breaks (dsb) was measured in cultured cells with pulsed-field gel electrophoresis after radiation doses in the range of 5-30 Gy. Human glioma, U-343MG and Chinese hamster, V79, cells were irradiated with either accelerated nitrogen ions of high linear energy transfer, LET approximately 125 keV/ microns, or photons from 60Co. The induction frequencies of dsb were similar for the two radiation qualities with a relative biological effectiveness, RBE, of 0.90 and 0.89 for the human and hamster cell lines respectively. The biphasic rejoining kinetics differed significantly between the two radiation qualities when studied in the human glioma cells. The difference was seen within the first hour after irradiation and after 6 h there were considerable differences in both the total amount of unrejoined dsb and the fraction of dsb rejoined during the slow phase. When rejoining was analysed 20-22 h after irradiation, the nitrogen ions gave 2.5-2.9 times more residual dsb than the gamma photons. The results for the hamster V79 cells were, up to 2h after irradiation, similar, but the difference between the two radiation qualities was less accentuated. In summary, similar initial yields of dsb after exposure of cells to high or low LET resulted in both radiation quality and cell type-dependent differences when the rejoining of these breaks were compared.
authors.affiliation: Division of Biomedical Radiation Sciences, Uppsala University, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Stenerlöw
authors.forename: E
authors.initials: E
authors.lastname: Blomquist
authors.forename: E
authors.initials: E
authors.lastname: Grusell
authors.forename: T
authors.initials: T
authors.lastname: Hartman
authors.forename: J
authors.initials: J
authors.lastname: Carlsson
mesh_terms: D000818:Animals; D003037:Cobalt Radioisotopes; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D016521:Electrophoresis, Gel, Pulsed-Field; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005910:Glioma / Q000378:metabolism; D006801:Humans; D007477:Ions; D007700:Kinetics; D018499:Linear Energy Transfer; D009584:Nitrogen; D010315:Particle Accelerators; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000528:radiation effects
chemical_list: D003037:Cobalt Radioisotopes; D007477:Ions; D004247:DNA; D009584:Nitrogen