Teaching light scattering spectroscopy: the dimension and shape of tobacco mosaic virus.
The tobacco mosaic virus is used as a model molecular assembly to illustrate the basic potentialities of light scattering techniques (both static and dynamic) to undergraduates. The work has two objectives: a pedagogic one (introducing light scattering to undergraduate students) and a scientific one (stabilization of the virus molecular assembly structure by the nucleic acid). Students are first challenged to confirm the stabilization of the cylindrical shape of the virus by the nucleic acid, at pH and ionic strength conditions where the coat proteins alone do not self-assemble. The experimental intramolecular scattering factor is compared with the theoretical ones for several model geometries. The data clearly suggest that the geometry is, in fact, a rod. Comparing the experimental values of gyration radius and hydrodynamic radius with the theoretical expectations further confirms this conclusion. Moreover, the rod structure is maintained over a wider range of pH and ionic strength than that valid for the coat proteins alone. The experimental values of the diffusion coefficient and radius of gyration are compared with the theoretical expectations assuming the dimensions detected by electron microscopy techniques. In fact, both values are in agreement (length approximately 300 nm, radius approximately 20 nm).
Santos
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NC
Departamento de Química e Bioquímica, Faculdade de Ciências de Universidade de Lisboa, Portugal.
Castanho
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MA
D001703:Biophysics / Q000193:education*; D008027:Light; D008962:Models, Theoretical; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods*; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure*