title: Cytomegalovirus infection in human immunodeficiency virus type 1-infected children.
abstract: BACKGROUND
  Cytomegalovirus (CMV) is a frequent opportunistic infection in human immunodeficiency virus type 1 (HIV-1)-infected children. The interactions of CMV and HIV-1 in coinfected children are not well-characterized.
  
  
  OBJECTIVE
  To evaluate the prevalence of asymptomatic CMV infection and symptomatic CMV disease and to assess the influence of CMV on clinical and laboratory markers of HIV disease progression in CMV-coinfected children.
  
  
  METHODS
  Serial urine CMV cultures were performed on 500 children (131 HIV-1-infected (HIV+), 129 seroreverters born to HIV-infected mothers, and 240 HIV-uninfected (HIV-)). The clinical, immunologic and virologic data of 131 HIV+ children were analyzed.
  
  
  RESULTS
  CMV was recovered in 40 of 131 HIV+ (31%), 22 of 129 seroreverters (17%) and 30 of 240 HIV- (13%) children. Of the 40 HIV+ children with CMV coinfection, 7 developed symptomatic CMV disease (17.5%) including chorioretinitis (3), colitis (2) and pneumonitis (2). The HIV+ children with symptomatic CMV disease had significantly lower mean CD4+ T lymphocyte proportions (17% vs. 26%; age-adjusted P = 0.013) and greater HIV p24 antigen concentrations (329 pg/ml vs. 57 pg/ml; age-adjusted P = 0.13) than HIV+ children with asymptomatic CMV infection. In a subset of children coinfected with CMV before 6 months of age (n = 11), 5 (45%) developed symptomatic CMV disease, and 4 of these 5 children died within 10 months of diagnosis of CMV disease. At the time of the first positive CMV culture in these children, mean CD4+ T lymphocyte proportions did not differ according to the presence or absence of CMV-related symptoms (symptomatic CMV+, 21% vs. asymptomatic CMV = 38%; P = 0.14). In HIV+ children with symptomatic CMV disease, p24 antigen concentrations were greater than in those with asymptomatic CMV infection (461 vs. 190 pg/ml, P = 0.06).
  
  
  CONCLUSIONS
  Symptomatic CMV disease occurred in young CMV-coinfected children with low CD4+ lymphocytes and elevated HIV p24 antigen concentrations. Whether progressive immunodeficiency allows the emergence of CMV disease or CMV infection causes more rapidly progressive HIV-1 disease or whether there is a more complex relationship remains to be determined.
authors.affiliation: Department of Pediatrics, New York University Medical Center, NY 10016, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chandwani
authors.forename: A
authors.initials: A
authors.lastname: Kaul
authors.forename: D
authors.initials: D
authors.lastname: Bebenroth
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.forename: D D
authors.initials: DD
authors.lastname: John
authors.forename: A
authors.initials: A
authors.lastname: Fidelia
authors.forename: A
authors.initials: A
authors.lastname: Hassel
authors.forename: W
authors.initials: W
authors.lastname: Borkowsky
authors.forename: K
authors.initials: K
authors.lastname: Krasinski
mesh_terms: D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis / Q000652:urine; D018450:Disease Progression; D015658:HIV Infections / Q000150:complications* / Q000175:diagnosis; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn