Use of arbitrarily primed polymerase chain reaction to differentiate Trichophyton dermatophytes.
Dermatophytes such as Trichophyton species are common human pathogens, the infection of which results in dermatophytosis (also known as ringworm). Several laboratory tests are used routinely for the diagnosis of dermatophytosis, but they are either slow or lacking specificity. Through examination of genomic DNA from Trichophyton dermatophytes and other fungi in arbitrarily primed PCR, it was shown that a random primer 5'-ACCCGACCTG-3' produced bands of 4.3 kb, 1.9 kb, 1.7 kb and 0.7 kb in T. rubrum DNA, bands of 2.5 kb, 1.9 kb and 0.8 kb in T. mentagrophytes var. interdigitale and T. mentagrophytes var. mentagrophytes DNA, and bands of 2.5 kb, 1.9 kb, 1.5 kb and 0.9 kb in T. tonsurans DNA. This primer amplified bands of different sizes in other fungal DNA. Therefore, based on the distinct band patterns observed in arbitrarily primed PCR using this primer, T. rubrum, T. mentagrophytes and T. tonsurans dermatophytes could be rapidly differentiated.
Liu
D
D
Melbourne Pathology, Collingwood, Vic, Australia.
Coloe
S
S
Pedersen
J
J
Baird
R
R
D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D003881:Dermatomycoses / Q000175:diagnosis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014249:Trichophyton / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D004271:DNA, Fungal