The effect of tilsuprost on the liver mitochondria in taurocholate pancreatitis in rats with antecedent acute ethanol abuse.
The damage to the liver appears to be an important aspect of multisystem organ failure in acute pancreatitis with poor prognosis. The objective of this study was to evaluate the protective effect of stable prostacyclin analogue--tilsuprost on the liver energy metabolism in taurocholate pancreatitis in rats preceded by acute ethanol intake. The respiratory control ratio (RCR) and ADP/O ratio of liver mitochondria with glutamate+malate as substrates and mitochondrial DNP (uncoupler)-dependent ATPase activity were significantly depressed after 12 h of taurocholate pancreatitis-the effects that were not significantly aggravated by antecedent acute ethanol intake. Tilsuprost (0.3 mg/kg i.g.) given just before induction of pancreatitis partly prevented the impairment of mitochondrial oxidative and phosphorylative functions, however these positive effects were limited in acute pancreatitis preceded by acute ethanol intake. These results suggest that prostacyclin analogues could be effective in the treatment of hepatic complications in acute pancreatitis, however their effectiveness could be limited in the case of acute ethanol antecedent abuse.
Dlugosz
J W
JW
Gastroenterology Department, Medical Academy, Bialystok, Poland.
Poplawski
C
C
Pawlicka
E
E
Wroblewski
E
E
Gabryelewicz
A
A
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000437:Alcoholism / Q000150:complications*; D000818:Animals; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010100:Oxygen / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced / Q000150:complications / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid / Q000633:toxicity*
D008293:Malates; D018698:Glutamic Acid; D013656:Taurocholic Acid; C030298:malic acid; C101919:tilsuprost; D011464:Epoprostenol; D000251:Adenosine Triphosphatases; D010100:Oxygen