Interconverting receptor states at 4 degrees C for the neutrophil N-formyl peptide receptor.
With the aid of high time resolution kinetic data extracted from a flow cytometer, we determined that there are two N-formyl peptide receptor states for human neutrophils at 4 degrees C: a low affinity and a high affinity state. Competitive binding of FMLP, FNLP, and t-BOC with FNLPNTL-FL revealed different kinetic rate constants for two distinct reactions that control the lifetime of the low affinity ligand-receptor complex. For these ligands, the rate constant for dissociation of ligand from the low affinity receptor state (the first reaction) ranges in order of magnitude from 10(-2) to 1 s-1, and the conversion rate constant from the low affinity receptor state to the high affinity receptor state (the second reaction) ranges from 10(-4) to 10(-2) s-1. The antagonist t-BOC differed most significantly from the three agonists by having an association rate constant for the low affinity receptor on the order of 10(5) M-1 s-1; the value for all three agonists was on the order of 10(7) M-1 s-1. Characterization of the receptor conversion at 4 degrees C revealed that it is irreversible (or very slow) and independent of Gi protein and that neither receptor state is a form of receptor precoupled to Gi protein. The affinity conversion and the dissociation characteristics of each receptor state determine the duration of the signaling complex and may contribute to differences in ligand efficacy.
Hoffman
J F
JF
Department of Chemical Engineering, University of Michigan, Ann Arbor, USA.
Keil
M L
ML
Riccobene
T A
TA
Omann
G M
GM
Linderman
J J
JJ
D001667:Binding, Competitive; D004151:Dipeptides / Q000378:metabolism; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D018000:Receptors, Peptide / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D004151:Dipeptides; D005452:Fluoresceins; D008024:Ligands; C097577:N-formyl-norleucyl-phenylalanine; C078594:N-formylnorleucyl-leucyl-phenylalanyl-norleucyl-tyrosyl-lysine receptor; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C061989:F-chemotactic peptide-fluorescein; C081709:tert-butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanyl-OH; D009240:N-Formylmethionine Leucyl-Phenylalanine; C021642:F-chemotactic peptide; D019206:GTP-Binding Protein alpha Subunits, Gi-Go