The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily.
We have cloned proteins that interact with the nuclear orphan receptor RZR beta using the yeast two-hybrid system. We identified, amongst a number of other genes, the nucleoside diphosphate kinase (NDPK)-2 also known as Nm23-2, c-myc regulatory factor PuF and differentiation inhibitory factor, RZR beta specifically interacts with Nm23-2 but not with the closely related tumor metastasis suppressor candidate gene product Nm23-1. In contrast ROR alpha interacts with both Nm23 proteins. These findings were corroborated by in vitro interaction assays based on GST-pulldown experiments. With-n-myc we propose a candidate gene regulated by ROR alpha/RZR beta and Nm23, based on the finding that the respective DNA binding sites in the first intron are conserved in several mammalian species.
Paravicini
G
G
Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S.A, Plan-les-Ouates, Switzerland.
Steinmayr
M
M
André
E
E
Becker-André
M
M
D000818:Animals; D001483:Base Sequence; D016259:Genes, myc; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011485:Protein Binding; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D054778:NM23 Nucleoside Diphosphate Kinases; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins