Cautionary note on the use of dUMP-containing PCR primers with Pfu and VentR DNA polymerases.
Sakaguchi
A Y
AY
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284, USA. sakaguchi@uthscsa.edu
Sedlak
M
M
Harris
J M
JM
Sarosdy
M F
MF
D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular / Q000379:methods*; D045647:DNA Glycosylases; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D013379:Substrate Specificity; D051981:Uracil-DNA Glycosidase
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D003856:Deoxyuracil Nucleotides; D005656:Fungal Proteins; D009687:Nuclear Proteins; C007267:2'-deoxyuridylic acid; C072146:Pfu DNA polymerase; C070331:Tli polymerase; D004259:DNA-Directed DNA Polymerase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase