Antigenic analysis of type O foot-and-mouth disease virus in the persistently infected bovine.
The antigenic profiles of serotype O strains of FMDV collected from the oropharynx of persistently infected cattle were defined with a panel of monoclonal antibodies (mAb's) in an indirect antigen-trapping ELISA. The mAb profiling showed no significant loss of reactivity in two neutralising antigenic sites of persistent FMDV isolates collected over a period of eight months. Early and late serum taken from a carrier animal showed similar neutralising activity against early and late carrier isolates. The antigenic stability of serotype O strains of FMDV collected during the carrier state suggests that antigenic variation facilitated by pressure from the host humoral immune response is not critical to the establishment or maintenance of a persistent infection with FMDV.
Salt
J S
JS
Institute for Animal Health, Pirbright Laboratory, Pirbright, U.K.
Samuel
A R
AR
Kitching
R P
RP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology* / Q000302:isolation & purification; D002353:Carrier State / Q000276:immunology; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000821:virology*; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral