title: Brain and pituitary melatonin receptors in male rat during post-natal and pubertal development and the effect of pinealectomy and testosterone manipulation.
abstract: Using quantitative autoradiography, melatonin receptors have been studied during post-natal and pubertal development of the rat in 2 brain and 2 pituitary structures. In the pars distalis of anterior pituitary, melatonin receptors decrease gradually in density after birth and disappear in 30 day-old animals. In contrast melatonin binding is only expressed in the paraventricular nuclei of the thalamus at the age of 21-23 days and is always present in adult animals. In the suprachiasmatic nuclei and in the pars tuberalis of the pituitary, melatonin receptor density decreases after birth, remains stable for approximately 1 month and increases again at puberty to reach the birth values in the adult. This increase was absent in pinealectomized and in castrated animals but present in castrated animals receiving testosterone suggesting that it depends upon circulating testosterone and melatonin levels. These results show that melatonin receptors are differentially regulated during post-natal development in each of the 4 structures studied, and that melatonin and testosterone are 2 factors which could be involved in the regulation of melatonin receptor density in the suprachiasmatic nuclei and pars tuberalis.
authors.affiliation: URA CNRS 1332, Université Louis Pasteur, Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Zitouni
authors.forename: P
authors.initials: P
authors.lastname: Pévet
authors.forename: M
authors.initials: M
authors.lastname: Masson-Pévet
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005069:Evaluation Studies as Topic; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects; D010870:Pineal Gland / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000032:analysis / Q000187:drug effects*; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis / Q000187:drug effects*; D044094:Receptors, Melatonin; D012741:Sexual Maturation; D013493:Suprachiasmatic Nucleus / Q000187:drug effects; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin; D013739:Testosterone