title: Reduction of intestinal apo A-IV mRNA levels in the cirrhotic rat.
abstract: In the present study, intestinal apo A-IV synthesis was investigated using a carbon tetrachloride (CCl4)-induced cirrhosis rat model. Triglyceride (TG) content in rat cirrhotic liver was increased markedly by 170% (P < 0.001) and apo B was increased by 20% (P < 0.05) compared with control levels. These results reflected the steatotic change in the liver. In contrast, TG levels in the small intestine of cirrhotic rats decreased significantly (P < 0.01). In addition, intestinal apo A-IV (jejunum P < 0.001; ileum P < 0.01) and its mRNA levels (jejunum P < 0.01; ileum P < 0.05) were also reduced. The decreased apo A-IV content in the jejunum was confirmed by immunohistochemical analysis. These results indicate that intestinal apo A-IV synthesis in cirrhosis is suppressed, at least under the condition of an overnight fast. Therefore, decreased intestinal apo A-IV synthesis may relate to the decreased ability to absorb fat in cirrhosis, but a fat-loading study will be necessary to confirm this hypothesis. It is unknown from the present study why serum apo A-IV level is not significantly decreased, despite a reduction in apo A-IV synthesis. The clearance of apo A-IV by the liver may be delayed or apo A-IV synthesis may be rather markedly enhanced during fat absorption in liver cirrhosis.
authors.affiliation: Department of Laboratory Medicine, Gifu University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Seishima
authors.forename: T
authors.initials: T
authors.lastname: Usui
authors.forename: S
authors.initials: S
authors.lastname: Naganawa
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
authors.forename: H
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: A
authors.initials: A
authors.lastname: Noma
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000378:metabolism; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D002251:Carbon Tetrachloride; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001053:Apolipoproteins; D001054:Apolipoproteins A; D008055:Lipids; D012333:RNA, Messenger; C022205:apolipoprotein A-IV; D002251:Carbon Tetrachloride