Restriction endonuclease analysis of group B streptococcal isolates from two distinct geographical regions.
This study examined isolates of group B streptococci (GBS) by latex seroagglutination and restriction endonuclease analysis (REA). REA analysis was performed with high- and low-frequency cutting enzymes, and fragments were resolved by linear and pulsed-field gel electrophoresis (PFGE), respectively. GBS isolates were derived from a regional acute-care hospital and a tertiary referral obstetric hospital. The most common serotype isolated at the general hospital was serotype V (42%). A high incidence of this serotype V was associated with wound infections, but there was no predominant REA profile. Serotype III was the most common from the obstetric hospital (41%). The majority of these isolates were associated with neonatal GBS infection and there was a predominant restriction pattern. Analysis of restriction patterns of GBS isolated from individual mother/ baby pairs revealed common patterns. These data extend the evidence for vertical transmission of GBS and clones of serotype III GBS with increased pathogenic potential.
Ellis
S
S
Department of Medicine, University of Melbourne, Geelong Hospital, Victoria, Australia.
Kotiw
M
M
Garland
S M
SM
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D000091402:Prohibitins; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics*; D014739:Victoria
D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins; D004262:DNA Restriction Enzymes