Development of gene transfer methods for Rubrivivax gelatinosus S1: construction, characterization and complementation of a puf operon deletion strain.
Gene transfer systems were developed in Rubrivivax (Rx.) gelatinosus S1. First, a system for conjugative transfer of mobilizable plasmids from Escherichia coli to Rx. gelatinosus S1 was established. Secondly, optimal conditions for the transformation of Rx. gelatinosus S1 by electroporation were determined. A delta puf strain was constructed. Complementation with the puf operon from a wild-type strain cloned in a replicative plasmid restored photosynthetic growth. Two insertion strains were also selected. All the strains constructed were green, due to a change in carotenoid content. Characterization of these strains provides genetic evidence for a "superoperon" organization in this bacterium.
Ouchane
S
S
CNRS, Centre de Génétique Moléculaire, Gif sur Yvette, France.
Picaud
M
M
Reiss-Husson
F
F
Vernotte
C
C
Astier
C
C
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D045342:Light-Harvesting Protein Complexes; D010641:Phenotype; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012244:Rhodospirillaceae / Q000235:genetics*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; C094744:light-harvesting complex 1, Rhodospirillum