Molecular cloning and RNA expression of two new human chemokine receptor-like genes.
Two new human genes encoding putative G protein-coupled receptors were cloned from genomic DNA following a degenerate PCR strategy. The predicted amino acid sequences of the proteins encoded by these genes have the characteristic motifs of chemokine receptors. This prompted us to name these genes CKR-L1 and CKR-L3. Nevertheless, the identification of their ligands has not been possible using 16 human chemokines in three different assays. The RNA expression pattern of CKR-L1 and CKR-L3 is also similar to that generally found for chemokine receptors. To a different extent, these genes are expressed in spleen, lymph nodes, and CD4+, CD8+ and CD19+ lymphocytes. CKR-L1 expression was also detected in monocyte/macrophages. The homology to chemokine receptors suggests that CKR-L1 belongs to the family of beta chemokine receptors, while CKR-L3 is more similar to alpha chemokine receptors.
Zaballos
A
A
Research Department, Pharmacia & Upjohn, Madrid, Spain.
Varona
R
R
Gutiérrez
J
J
Lind
P
P
Márquez
G
G
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D054399:Receptors, CCR6; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
C106311:CCR6 protein, human; C103330:CCR8 protein, human; D008024:Ligands; D054399:Receptors, CCR6; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D012313:RNA