title: Phylogenetic analysis of tnpR genes in mercury resistant soil bacteria: the relationship between DNA sequencing and RFLP typing approaches.
abstract: The diversity of resolvase (tnpR) genes carried by a number of mercury resistant soil bacteria has been investigated by DNA sequencing. The resulting DNA sequence information was compared to previously published tnpR DNA sequences and to previously published restriction fragment length polymorphism (RFLP) data, permitting the relationships between DNA sequencing and RFLP approaches to be studied by the use of phylogenetic trees. DNA maximum likelihood and DNA parsimony were used to construct a variety of phylogenetic trees. DNA sequencing confirmed the validity of RFLP analysis and highlighted the importance of restriction endonuclease choice upon the resulting RFLP patterns and dendrogram topology. The tnpR genes of two previously uncharacterised mercury resistant bacteria, T2-7 and T2-12 were also studied. DNA sequence data placed T2-7 in a previously described gene class, tnpR-D and T2-12 in a new gene class, tnpR-F. The significance of this data with respect to the recombination and evolution events occurring within bacterial populations are discussed.
authors.affiliation: Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, UK. R.J.Holt@liverpool.ac.uk
authors.forename: R J
authors.initials: RJ
authors.lastname: Holt
authors.forename: P
authors.initials: P
authors.lastname: Strike
authors.forename: K D
authors.initials: KD
authors.lastname: Bruce
mesh_terms: D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000145:classification* / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D008628:Mercury / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D019895:Transposases
chemical_list: D004254:DNA Nucleotidyltransferases; D019895:Transposases; D008628:Mercury