title: Poor correlation between clonal immunoglobulin gene rearrangement and immunoglobulin gene transcription in Hodgkin's disease.
abstract: It recently has been suggested that some cases of Hodgkin's disease (HD) are derived from B lymphocytes. We therefore examined immunoglobulin heavy chain gene rearrangement and transcription by polymerase chain reaction in 30 cases of HD and compared them with 25 cases of non-Hodgkin's B-cell lymphoma. Clonal VDJ gene rearrangements were observed in 6 cases of HD. Of these 6 cases, clonal VDJ gene transcription was found by RNA-dependent polymerase chain reaction in only 2 cases. One of these 2 cases histologically showed composite lymphoma of HD and diffuse large B cell lymphoma. We performed in situ hybridization with a clonal VDJ gene probe in this case to determine the localization of the genetic clonality. The large lymphoma cells reacted positively, but Hodgkin's and Reed-Sternberg cells showed only nonspecific staining. No discrepancy between immunoglobulin gene rearrangement and transcription was observed in 24 of the 25 cases of non-Hodgkin's B-cell lymphoma. The discordance between clonal gene rearrangement and transcription in HD contrasts sharply with the high correlation of those processes in non-Hodgkin's B-cell lymphoma.
authors.affiliation: First Department of Pathology, Nagoya University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yatabe
authors.forename: K
authors.initials: K
authors.lastname: Oka
authors.forename: J
authors.initials: J
authors.lastname: Asai
authors.forename: N
authors.initials: N
authors.lastname: Mori
mesh_terms: D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D014158:Transcription, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains