Immunolocalization of human gelatinase (type IV collagenase, MMP-9) and tissue inhibitor of metalloproteinase 1 in Hailey-Hailey and Darier's diseases.
BACKGROUND
The formation of lacunae and acantholysis as well as dyskeratosis are characteristic features of Hailey-Hailey disease (HHD) and Darier's disease (DD). Matrix metalloproteinases (MMPs) and their inhibitors like tissue inhibitors of metalloproteinases (TIMPs) have been thought to play major roles in the tissue metabolism.


OBJECTIVE
The aim of this study was to investigate the role of MMP-9 and TIMP-1 in HHD and DD.


METHODS
We examined localizations of these two molecules by immunostaining using specific monoclonal antibodies.


RESULTS
MMP-9 was positively stained in dyskeratotic or detaching cells around lacunae in HHD and DD. TIMP-1 showed a positive staining pattern throughout the epidermis.


CONCLUSIONS
MMP-9 might be involved in the pathophysiological process of HHD and DD in the presence of TIMP-1.
Kobayashi
T
T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Sakuraoka
K
K
Hattori
S
S
Hori
H
H
Nagai
Y
Y
Tajima
S
S
Nishikawa
T
T
D000051:Acantholysis / Q000201:enzymology / Q000473:pathology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002448:Cell Adhesion; D002478:Cells, Cultured; D017364:Collagenases / Q000032:analysis*; D004396:Coloring Agents; D007644:Darier Disease / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000201:enzymology / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000473:pathology; D007642:Keratosis / Q000201:enzymology / Q000473:pathology; D020780:Matrix Metalloproteinase 9; D061965:Matrix Metalloproteinase Inhibitors; D016506:Pemphigus, Benign Familial / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D011480:Protease Inhibitors / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D019714:Tissue Inhibitor of Metalloproteinases
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D006023:Glycoproteins; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D012967:Sodium Dodecyl Sulfate; D017364:Collagenases; D020780:Matrix Metalloproteinase 9