Trophic effects of interleukin-11 in rats with experimental short bowel syndrome.
Interleukin-11 (IL-11) is a multifunctional cytokine, derived from bone marrow stromal cells, that stimulates proliferation of stem/progenitor precursor cells in the small intestinal crypts and accelerates recovery of intestinal mucosa after cytoablative therapy. This study evaluates whether IL-11 can improve the function and structure of the small intestine and enhance adaptation in an experimental model of short bowel syndrome. After 90% small bowel resection, 32 Sprague-Dawley rats were divided randomly into eight experimental groups of four animals each. Four groups were treated with IL-11 (125 micrograms/kg twice daily, subcutaneously), and the four control groups were treated with a similar volume (0.1%) of bovine serum albumin (BSA). The animals were weighed daily and were killed on day 2, 4, 6, or 8; remnant small bowel was evaluated for villus height and crypt cell mitosis. The body weight of the animals that received IL-11 was significantly greater at the beginning of postoperative day 4 in comparison to that of the BSA groups (P < .01 during days 5 to 7). The rats that had IL-11 also had significantly greater villus height and crypt cell mitotic rates (P < .05). These observations suggest that IL-11 has a trophic effect on the small bowel during the adaptive phase that follows massive bowel resection and may be useful in the treatment of short bowel syndrome.
Liu
Q
Q
Department of Surgery, Indiana University School of Medicine, Herman B. Wells Center for Pediatric Research, USA.
Du
X X
XX
Schindel
D T
DT
Yang
Z X
ZX
Rescorla
F J
FJ
Williams
D A
DA
Grosfeld
J L
JL
D000222:Adaptation, Physiological / Q000276:immunology*; D000818:Animals; D001835:Body Weight; D002417:Cattle; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D017370:Interleukin-11 / Q000276:immunology* / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000166:cytology / Q000254:growth & development / Q000276:immunology; D008297:Male; D008938:Mitosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000627:therapeutic use; D012778:Short Bowel Syndrome / Q000188:drug therapy* / Q000276:immunology*
D017370:Interleukin-11; D012710:Serum Albumin, Bovine