title: Growth enhancement in transgenic tilapia by ectopic expression of tilapia growth hormone.
abstract: The generation of transgenic fish with the transfer of growth hormone (GH) genes has opened new possibilities for the manipulation of growth in economically important fish species. The tilapia growth hormone (tiGH) cDNA was linked to the human cytomegalovirus (CMV) enhancer-promoter and used to generate transgenic tilapia by microinjection into one-cell embryos. Five transgenic tilapia were obtained from 40 injected embryos. A transgenic animal containing one copy of the transgene per cell was selected to establish a transgenic line. The transgene was stably transmitted to F1 and F2 generations in a Mendelian fashion. Ectopic, low-level expression of tiGH was detected in gonad and muscle cells of F1 transgenic tilapia by immunohystochemical analysis of tissue sections. Nine-month-old transgenic F1 progeny were 82% larger than nontransgenic fish at p = .001. These results showed that low-level ectopic expression of tiGH resulted in a growth acceleration in transgenic tilapia. Tilapia GH gene transfer is an alternative for growth acceleration in tilapia.
authors.affiliation: Mammalian Cell Genetics Division, Centro de Ingeniería Genética y Biotechnologia, Havana, Cuba.
authors.forename: R
authors.initials: R
authors.lastname: Martínez
authors.forename: M P
authors.initials: MP
authors.lastname: Estrada
authors.forename: J
authors.initials: J
authors.lastname: Berlanga
authors.forename: I
authors.initials: I
authors.lastname: Guillén
authors.forename: O
authors.initials: O
authors.lastname: Hernández
authors.forename: E
authors.initials: E
authors.lastname: Cabrera
authors.forename: R
authors.initials: R
authors.lastname: Pimentel
authors.forename: R
authors.initials: R
authors.lastname: Morales
authors.forename: F
authors.initials: F
authors.lastname: Herrera
authors.forename: A
authors.initials: A
authors.lastname: Morales
authors.forename: J C
authors.initials: JC
authors.lastname: Piña
authors.forename: Z
authors.initials: Z
authors.lastname: Abad
authors.forename: V
authors.initials: V
authors.lastname: Sánchez
authors.forename: P
authors.initials: P
authors.lastname: Melamed
authors.forename: R
authors.initials: R
authors.lastname: Lleonart
authors.forename: J
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001824:Body Constitution; D001835:Body Weight; D003587:Cytomegalovirus / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006066:Gonads / Q000737:chemistry; D013006:Growth Hormone / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008297:Male; D009132:Muscles / Q000737:chemistry; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D017210:Tilapia / Q000235:genetics* / Q000254:growth & development*
chemical_list: D013006:Growth Hormone