title: 1 alpha,25-dihydroxyvitamin D3 stimulated alkaline phosphatase activity in cultured pig kidney epithelial LLC-PK1 cells.
abstract: The effects of 1 alpha,25-dihydroxyvitamin D3 (1,25 (OH)2D3) and human growth hormone (hGH) on the activity of alkaline phosphatase (ALP) were evaluated in pig kidney LLC-PK1 cultured cells. 1,25(OH)2D3 (10(-9)-10(-6) mol L-1) and hGH (10(-7) mol L-1) increased ALP activity in these cells while no hormonal effect was detected on two other enzyme activities: gamma-glutamyl transferase and acid phosphatase. ALP activity was maximally increased after 4.5-6 h incubation with both hormones. The hormonal induction of ALP activity was prevented by a pretreatment of cells with actinomycin D. Thus, 1,25 (OH)2D3 and may be hGH could stimulate ALP activity via a transcription of some gene.
authors.affiliation: Laboratoire de Biochimie/Physiologie, UFR de Médecine/Pharmacie, Université d'Angers, France.
authors.forename: X
authors.initials: X
authors.lastname: Do Thanh
authors.forename: F
authors.initials: F
authors.lastname: Massicot
authors.forename: B
authors.initials: B
authors.lastname: Do
authors.forename: R
authors.initials: R
authors.lastname: Breget
authors.forename: V
authors.initials: V
authors.lastname: Nivet
authors.forename: D
authors.initials: D
authors.lastname: Durand
authors.forename: J M
authors.initials: JM
authors.lastname: Warnet
authors.forename: J R
authors.initials: JR
authors.lastname: Claude
authors.forename: J P
authors.initials: JP
authors.lastname: Clot
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002117:Calcitriol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D013006:Growth Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D018374:LLC-PK1 Cells; D012313:RNA / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013552:Swine; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003609:Dactinomycin; D012313:RNA; D013006:Growth Hormone; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002117:Calcitriol