A comparative evaluation of three detectors in the HPLC analysis of sodium fusidate.
A reversed phase liquid chromatographic (HPLC) method is proposed for the determination of sodium fusidate, a steroid antibiotic, in a commercial ointment using three different detectors: a) fluorescence detector, after a preliminary derivatization with 2-bromoacetyl-6-methoxynaphthalene (Br-AMN), b) UV detection and c) evaporative light scattering detector (ELSD). Fluorescent derivatization was applied directly to the diluted sample, without any purification, whilst UV and ELSD detectors involved a preliminary liquid-liquid extraction procedure to obtain accurate analysis data.
Gatti
R
R
Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Italy.
Gotti
R
R
Bonazzi
D
D
Cavrini
V
V
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D005672:Fusidic Acid / Q000032:analysis*; D008027:Light; D012542:Scattering, Radiation; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D005672:Fusidic Acid