title: Retinoic acid induces cell proliferation and modulates gelatinases activity in human osteoclast-like cell lines.
abstract: The effect of Retinoic Acid (RA) on human osteoclast-like cell lines, obtained from Giant Cell tumors (GCT) of bone, has been investigated evaluating its action on bone resorption, cell proliferation, microtubular organization and gelatinases expression and activity. Increasing concentrations of RA significantly dose-dependently decreased GCTs bone resorption, while 10(-7) M RA promoted an increase of cell proliferation. By immunofluorescence we demonstrated that GCTs express A and B gelatinases and, by zymography, that their activity was enhanced in medium collected from GCTs cultured in the presence of 10(-7) M RA. These data indicate that RA increases cell proliferation and modulates metalloproteinases (MMPs) activity, crucial events during the migration of osteoclast precursors toward bone surfaces.
authors.affiliation: Institute of Human Anatomy, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Colucci
authors.forename: M
authors.initials: M
authors.lastname: Grano
authors.forename: G
authors.initials: G
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Scotlandi
authors.forename: M
authors.initials: M
authors.lastname: Mastrogiacomo
authors.forename: C
authors.initials: C
authors.lastname: Mori
authors.forename: A Z
authors.initials: AZ
authors.lastname: Zallone
mesh_terms: D001862:Bone Resorption; D002455:Cell Division / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D010010:Osteoclasts / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium; D014407:Tumor Cells, Cultured
chemical_list: D014316:Tritium; D014212:Tretinoin; D018093:Gelatinases; D013936:Thymidine