title: Bronchoalveolar and systemic cytokine profiles in patients with ARDS, severe pneumonia and cardiogenic pulmonary oedema.
abstract: The aim of this study was to investigate whether bronchoalveolar lavage (BAL) and serum levels of proinflammatory cytokines discriminate between different entities of patients with acute respiratory failure. BAL and circulating concentrations of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) were measured in 74 mechanically-ventilated patients and 17 healthy controls. Patients were classified as cardiogenic pulmonary oedema (CPO), acute respiratory distress syndrome (ARDS), primary severe pneumonia (PN) and a combined group (PN+ARDS). In all patients with ARDS and/or PN, markedly elevated BAL levels of IL-6 and IL-8 were detected, which were significantly greater than levels in CPO and healthy controls. Absolute quantities and time-course of these cytokines did not differentiate between the absence and presence of lung infection, or different categories of PN. Similarly, circulating IL-6 levels were comparably elevated in patients with ARDS and/or PN, whereas circulating IL-8 concentrations were inconsistently increased. TNF-alpha was rarely detected in BAL samples, but increased serum concentrations were measured in ARDS and/or PN patients. Bronchoalveolar lavage levels of interleukin-6 and interleukin-8, but not tumour necrosis factor-alpha, and serum concentrations of interleukin-6 are consistently elevated in acute respiratory distress syndrome and/or severe pneumonia, discriminating these entities from cardiogenic pulmonary oedema. Alveolar and systemic cytokine profiles do not differentiate between acute respiratory distress syndrome in the absence of lung infection and states of severe primary or secondary pneumonia, which evidently present with comparable local and systemic inflammatory sequelae.
authors.affiliation: Dept of Internal Medicine, Justus-Liebig University, Giessen, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Schütte
authors.forename: J
authors.initials: J
authors.lastname: Lohmeyer
authors.forename: S
authors.initials: S
authors.lastname: Rosseau
authors.forename: S
authors.initials: S
authors.lastname: Ziegler
authors.forename: C
authors.initials: C
authors.lastname: Siebert
authors.forename: H
authors.initials: H
authors.lastname: Kielisch
authors.forename: H
authors.initials: H
authors.lastname: Pralle
authors.forename: F
authors.initials: F
authors.lastname: Grimminger
authors.forename: H
authors.initials: H
authors.lastname: Morr
authors.forename: W
authors.initials: W
authors.lastname: Seeger
mesh_terms: D000208:Acute Disease; D001424:Bacterial Infections / Q000097:blood / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000276:immunology; D002303:Cardiac Output, Low / Q000150:complications; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000097:blood; D016209:Interleukin-8 / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000097:blood / Q000378:metabolism*; D018410:Pneumonia, Bacterial / Q000097:blood / Q000378:metabolism; D011175:Positive-Pressure Respiration; D011654:Pulmonary Edema / Q000097:blood / Q000378:metabolism*; D011660:Pulmonary Heart Disease / Q000097:blood / Q000378:metabolism; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D012131:Respiratory Insufficiency / Q000097:blood / Q000378:metabolism; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha