Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat.
A simple and efficient procedure for the dispersion and culture of magnocellular neurons of the adult rat hypothalamus was developed. The enzymatically and mechanically dispersed cells were highly viable and showed neurite outgrowth after 3 days of culture. The neurons could be maintained for more than 4 weeks without any sign of deterioration. Light-optic immunocytochemistry of the cultured cells revealed the presence of many oxytocin- and vasopressin-immunoreactive neurons. The results indicate that the method may be suitable for many experimental purposes.
Bilinski
M
M
Instituto de Neurobiología, Buenos Aires, Argentina.
Sánchez
A
A
González Nicolini
V
V
Villar
M J
MJ
Tramezzani
J H
JH
D000818:Animals; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010121:Oxytocin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013495:Supraoptic Nucleus / Q000166:cytology*; D014667:Vasopressins / Q000378:metabolism
D014667:Vasopressins; D010121:Oxytocin