Vascular cell adhesion molecule-1 mRNA is expressed in immune-mediated and ischemic injury of the rat nervous system.
In this study we used nonradioactive in situ hybridization for the cellular localization of vascular cell adhesion molecule-1 (VCAM-1) mRNA in immune-mediated, ischemic and degenerative diseases of the rat nervous system. In the acute phase of experimental autoimmune encephalomyelitis and neuritis VCAM-1 mRNA was expressed not only on the luminal surface of inflamed vessels but also in perivascular cells suggesting a functional role of VCAM-1 in both endothelial adhesion and local restimulation of autoantigen-specific T cells. Accordingly, perivascular T cell accumulation was most pronounced at sites of local VCAM-1 mRNA expression. In addition, VCAM-1 mRNA was detected in the border zone around photochemically induced cerebral infarcts which is the predeliction site of T cell infiltration and expression of immune activation markers during the first week after ischemia. VCAM-1 mRNA was absent from the center of the infarcts as well as axotomized central and peripheral nerves undergoing Wallerian degeneration. These data indicate that VCAM-1-mediated adhesion processes are involved in immune-mediated and ischemic diseases of the nervous system but not in T cell-independent macrophage recruitment during Wallerian degeneration.
Jander
S
S
Department of Neurology, Heinrich-Heine-University, Düsseldorf, Germany.
Pohl
J
J
Gillen
C
C
Schroeter
M
M
Stoll
G
G
D000208:Acute Disease; D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D002417:Cattle; D002536:Cerebral Arteries / Q000378:metabolism* / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D007114:Immunization; D017403:In Situ Hybridization; D002542:Intracranial Embolism and Thrombosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation; D009409:Nerve Crush; D009444:Neuritis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009900:Optic Nerve / Q000378:metabolism; D020221:Optic Nerve Injuries; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics*; D014855:Wallerian Degeneration
D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1