Assay of free-radical toxicity and antioxidant effect on the Hep 3B cell line: a test survey using lindane.
The content of reduced glutathione and of glutathione disulfide as well as the activities of glutathione reductase, glutathione peroxidase, glutathione S-transferases, catalase and superoxide dismutases were determined in human hepatoma Hep 3B cells in relation to free-radical toxicity in order to appreciate the defense capacities of these cells compared to data on normal hepatocytes. When Hep 3B cells were exposed to lindane, a known inducer of free-radical production, superoxide dismutase activity appeared as the best-adapted cellular parameter for early detection of the resulting free-radical toxicity.
Descampiaux
B
B
Département de Toxicologie, Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France.
Leroux
J M
JM
Peucelle
C
C
Erb
F
F
D000975:Antioxidants / Q000633:toxicity*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D005609:Free Radicals / Q000633:toxicity; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000473:pathology; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000032:analysis / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000633:toxicity
D000975:Antioxidants; D005609:Free Radicals; D014810:Vitamin E; D001556:Hexachlorocyclohexane; D013482:Superoxide Dismutase