Evaluation of numerical analysis of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus plantarum and Lactobacillus pentosus.
Lactobacillus plantarum and Lactobacillus pentosus grouped into one protein profile cluster at r > or = 0.70, separate from Lactobacillus casei, Lactobacillus sake, and Lactobacillus curvatus. Similar sugar fermentation reactions were recorded for representative strains of L. plantarum and L. pentosus. Representative strains, including the type of each species, were selected from the different protein profile clusters and their genetic relatedness determined by using numerical analysis of random amplified polymorphic DNA (RAPD)-PCR. The type strains of L. plantarum (ATCC 14917T) and L. pentosus (NCFB 363T) displayed different RAPD profiles and grouped into two independent clusters, well separated from L. casei, L. curvatus, and L. sake. Numerical analysis of RAPD-PCR proved a reliable and accurate method to distinguish between strains of L. plantarum and L. pentosus.
Van Reenen
C A
CA
Department of Microbiology, University of Stellenbosch, South Africa.
Dicks
L M
LM
D001483:Base Sequence; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003627:Data Interpretation, Statistical; D005285:Fermentation; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000706:statistics & numerical data*; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial