Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants.
Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.
Rosengren
A
A
Department of Physiology and Neuroscience, Lund University, Sweden.
Johansson
B R
BR
Danielsen
N
N
Thomsen
P
P
Ericson
L E
LE
D000009:Abdominal Muscles; D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis*; D014025:Titanium
D001672:Biocompatible Materials; D005353:Fibronectins; D007074:Immunoglobulin G; D012709:Serum Albumin; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D003094:Collagen; D014025:Titanium