title: Histamine-and isoprenaline-evoked stimulation of cAMP formation in chick cerebral cortex.
abstract: Histamine (HA) and isoprenaline (ISO) strongly stimulated cAMP formation in chick cerebral cortical slices. The effects of HA and ISO were selectively antagonized by HA H2-receptor blockers (aminopotentidine, ranitidine) and beta-adrenoceptor blocker propranolol, respectively. The combination of HA (0.1-100 microM) and ISO (0.1-10 microM) produced some additive effects when the drugs were used at low concentrations, i.e. 0.1-1 microM but not when HA was applied at 10 or 100 microM doses (which evoked nearly maximal effect). It is suggested that HA H2-like receptors and beta-adrenoceptors (whose activation leads to stimulation of cAMP formation) are localized in the same subpopulation of cortical cells, in which they probably utilize a common and limited pool of cAMP-generating system.
authors.affiliation: Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Nowak
authors.forename: J B
authors.initials: JB
authors.lastname: Zawilska
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004347:Drug Interactions; D006632:Histamine / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D006635:Histamine H2 Antagonists; D006632:Histamine; D000242:Cyclic AMP; D007545:Isoproterenol