The neutrophil oxidative burst in diarrhoea-associated haemolytic uraemic syndrome.
Neutrophil-mediated tissue damage has been implicated in the pathogenesis of diarrhoea-associated haemolytic uraemic syndrome (D+ HUS). This study evaluates priming and activation of the neutrophil oxidative burst in D+ HUS using chemiluminescent techniques. Peripheral blood neutrophils from 11 children with acute D+ HUS were examined. No difference was found in the oxidative burst of neutrophils from patients and controls. Serum elastase levels were measured in 8 patients and found to be significantly elevated. Although elastase results suggest neutrophil activation, chemiluminescence studies do not confirm this in the peripheral blood neutrophil. This does not support a significant role for circulating agents in priming and activating the peripheral blood neutrophil.
Hughes
D A
DA
Royal Hospital For Sick Children, Glasgow, Scotland, UK.
Smith
G C
GC
Davidson
J E
JE
Murphy
A V
AV
Beattie
T J
TJ
D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007223:Infant; D019272:Leukocyte Elastase / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D016897:Respiratory Burst / Q000502:physiology*
D019272:Leukocyte Elastase