Localization of Alzheimer-associated presenilin 1 in transfected COS-7 cells.
Presenilin 1 (PS 1) is the recently identified gene, located on chromosome 14, of which missense mutations can cause early-onset familial Alzheimer's disease. To understand the normal biological function of presenilin 1, we examined the sub-cellular localization by using a monoclonal anti-presenilin 1 antibody. Immuno-electronmicroscopic and biochemical analysis indicated that presenilin 1 is localized on cellular membrane (plasma, endoplasmic reticulum, and perinuclear) in COS-7 cells overexpressing presenilin 1. Interestingly, the PS 1 immunoreactivity in the plasma membrane was concentrated in the regions with cell-cell contact. This observation suggests a possible role of PS 1 on the cell membrane as a cell adhesion molecule.
Takashima
A
A
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Sato
M
M
Mercken
M
M
Tanaka
S
S
Kondo
S
S
Honda
T
T
Sato
K
K
Murayama
M
M
Noguchi
K
K
Nakazato
Y
Y
Takahashi
H
H
D000544:Alzheimer Disease / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal; D019556:COS Cells; D015815:Cell Adhesion Molecules; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D002883:Chromosomes, Human, Pair 14; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D016253:Microscopy, Immunoelectron; D053764:Presenilin-1; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D014162:Transfection
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D011994:Recombinant Proteins