Isolation and partial characterization of polymyxin B-resistant isolates of Vibrio parahaemolyticus.
Two polymyxin-resistant isolates V2PXR and P1R4PXR of Vibrio parahaemolyticus were spontaneously isolated and compared with the polymyxin-sensitive strains as to their chemical compositions of cell envelopes and their outer membrane protein compositions. When grown in the presence or absence of polymyxin, the cell envelopes of polymyxin-resistant isolates showed a significantly increased content of protein as compared with those of respective polymyxin-sensitive strains. Both polymyxin-resistant isolates produced the major outer membrane protein b' after growth in the presence or absence of polymyxin. A new major outer membrane protein with an apparent molecular weight of 26,000 (26 K protein) was produced by polymyxin-resistant isolates grown in the presence of polymyxin. The production of 26 K protein was also observed in the polymyxin-resistant isolates grown in the medium containing low concentrations of NaCl (0.2% and 0.5%).
Koga
T
T
Department of Food Microbiology, Tokushima University School of Medicine, Japan.
Takumi
K
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004352:Drug Resistance, Microbial; D011112:Polymyxin B / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D012965:Sodium Chloride; D011112:Polymyxin B