title: Transcriptional regulatory elements in the rat bradykinin B2 receptor gene.
abstract: In an attempt to elucidate the mechanisms underlying the regulation of bradykinin B2 receptor gene expression, the molecular structure of the rat gene including the 5'-flanking region was characterized (J. Biol. Chem. 269: 26920-26925, 1994). In this study we show that the gene spans about 32 kb, including a long first intron of 25 kb. The promoter region drives reporter gene expression in NG108-15 neuroglioma cells, and its expression is upregulated by cAMP, bradykinin, phorbol esters and by coexpression of an activated ras oncogene but not by dexamethasone.
authors.affiliation: Department of Biophysics, Escola Paulista de Medicina, Säo Paulo, Brazil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pesquero
authors.forename: C J
authors.initials: CJ
authors.lastname: Lindsey
authors.forename: A C
authors.initials: AC
authors.lastname: Paiva
authors.forename: D
authors.initials: D
authors.lastname: Ganten
authors.forename: M
authors.initials: M
authors.lastname: Bader
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001920:Bradykinin / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D011905:Genes, ras; D006801:Humans; D007438:Introns; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D017931:DNA Primers; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013755:Tetradecanoylphorbol Acetate; D001920:Bradykinin