title: Cytochrome P-450 and free radical generation in rat liver microsomes under the influence of prostaglandin E1.
abstract: There is evidence to suggest that the mechanism of antioxidant effect of prostaglandin E1 (PGE1) is due to decrease of radical species generation by cytochrome P-450 in rat liver microsomes. Chronic alcohol intoxication increased NADPH oxidation, cytochrome P-450 content and NADPH-stimulated chemoluminiscence of microsomes. Ethanol also raised superoxide dismutase (SOD) activity in microsomes. PGE1 decreased cytochrome P-450 content, normalized NADPH oxidation, NADPH-induced chemoluminiscence and SOD activity in the liver of alcohol-treated rats. PGE developed the similar effect after microsomal induction by both acetone combined with starvation and phenobarbital normalizing all the above parameters. Therefore, PGE1 affects on both, ethanol-inducible IIE1 and phenobarbital-inducible IIB1 isoforms.
authors.affiliation: Institute of Biochemistry, Belarussian Academy of Sciences, Grodno, Belarus.
authors.forename: V U
authors.initials: VU
authors.lastname: Buko
authors.forename: V V
authors.initials: VV
authors.lastname: Sadovnichy
mesh_terms: D000096:Acetone / Q000008:administration & dosage; D000527:Alprostadil / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D015536:Down-Regulation; D000431:Ethanol / Q000008:administration & dosage; D005609:Free Radicals / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D010634:Phenobarbital / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005609:Free Radicals; D000096:Acetone; D000431:Ethanol; D009249:NADP; D006861:Hydrogen Peroxide; D019392:Cytochrome P-450 CYP2E1; D019362:Cytochrome P-450 CYP2B1; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C069506:NADH-cytochrome P-450 reductase; D019255:NADPH Oxidases; D000527:Alprostadil; D010634:Phenobarbital