Expression of different forms of the heat-shock factor during the life cycle of the parasitic helminth Schistosoma mansoni.
HSE-HSF complexes from different stages of the life cycle of schistosome were analyzed by pore exclusion electrophoresis (estimates the size of the native DNA-protein complex), and by U.V.-cross-linking followed by SDS-PAGE (estimates the size of the monomeric heat shock factor, HSF). The apparent M(r) values of the native and monomeric HSE-HSF complexes from schistosomula were 80,000 and 60,000, respectively. In adult worms M(r) values were 70,000 for the native HSE-HSF complex, 60,000 and 80,000 for the monomeric form. These findings indicate that: (1) schistosome express two (maybe three) forms of HSF, (2) different forms of HSF are expressed at different developmental stages. The findings that the native HSE-HSF complex and the monomeric HSF are of a similar size indicates that the complex contains a single HSF, or that the complex is a labile oligomer of HSF that decomposes into monomers during electrophoresis in a nondenaturing gel.
Levy-Holtzman
R
R
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Schechter
I
I
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D015342:DNA Probes; D018509:DNA, Helminth / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012550:Schistosoma mansoni / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature; D014466:Ultraviolet Rays
D015342:DNA Probes; D018509:DNA, Helminth; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D001973:Bromodeoxyuridine