Myosin heavy chain mRNA and protein expression in single fibers of the rat soleus following reinnervation.
Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA in single fibers of the soleus. A midthigh sciatic nerve lesion resulted in reinnervation of the soleus by motoneurons from both original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements. At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.
Bodine
S C
SC
Biomedical Sciences Program, VA Medical Center, La Jolla 92093-9151, USA.
Pierotti
D J
DJ
D000818:Animals; D005260:Female; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000294:innervation* / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology
D009124:Muscle Proteins; D012333:RNA, Messenger; D018995:Myosin Heavy Chains