Role of prostaglandins and nitric oxide on halothane-induced arteriolar dilatation in rat diaphragm.
The effects of anaesthetics on the microcirculation of the diaphragm are incompletely understood. Therefore, we assessed by in vivo intravital microscopy in rats the action of halothane on diaphragmatic arteriolar diameter and the role of nitric oxide and prostaglandins on halothane-induced diaphragmatic arteriolar diameter. We studied 54 rats anaesthetized with thiopentone. Dose-response curves to topically applied Krebs' solution saturated with halothane at increasing concentrations of 0%, 1%, 3% and 5% were carried out in the presence of an inhibitor of nitric oxide synthesis (N omega-nitro-L-arginine (LNA), 300 mumol litre-1) or inhibitors of prostaglandin synthesis (mefenamic acid 20 mumol litre-1 or indomethacin 20 mol litre-1) or in the absence of any inhibitor. We found dose-dependent arteriolar dilatation which was abolished by mefenamic acid and indomethacin. In contrast, the effect of halothane was not modified by LNA. These data demonstrated that halothane-induced arteriolar dilatation in the diaphragm of the rat was mediated by prostaglandins but not by nitric oxide.
de Larminat
V
V
Faculté de Médecine X, Bichat, Paris, France.
Boczkowski
J
J
Dureuil
B
B
Vicaut
E
E
Farinotti
R
R
Aubier
M
M
Desmonts
J M
JM
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000502:physiology; D003964:Diaphragm / Q000098:blood supply*; D006221:Halothane / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008528:Mefenamic Acid / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
D018685:Anesthetics, Inhalation; D011453:Prostaglandins; D019335:Nitroarginine; D009569:Nitric Oxide; D008528:Mefenamic Acid; D019001:Nitric Oxide Synthase; D006221:Halothane; D007213:Indomethacin