title: Thogoto and Dhori virus replication is blocked by inhibitors of cellular polymerase II activity but does not cause shutoff of host cell protein synthesis.
abstract: Tick-transmitted Thogoto and Dhori viruses share structural and genetic properties with the influenza viruses. Here, we compare different steps of their replication cycle in mammalian cells in comparison with influenza A virus. Viral antigens of both viruses accumulated in the nuclei of infected cells, suggesting a nuclear phase of viral replication. Furthermore, as observed with influenza viruses, transcription of Thogoto and Dhori viruses was inhibited by alpha-amanitin and actinomycin D, suggesting a dependence of viral transcription on cellular RNA polymerase II activity. In contrast to influenza viruses, Thogoto and Dhori virus infection did not lead to down-regulation of cellular protein synthesis indicating marked differences regarding the fate of infected cells.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Siebler
authors.forename: O
authors.initials: O
authors.lastname: Haller
authors.forename: G
authors.initials: G
authors.lastname: Kochs
mesh_terms: D016475:3T3 Cells; D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D003609:Dactinomycin / Q000494:pharmacology*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009698:Nucleoproteins / Q000378:metabolism; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014176:Protein Biosynthesis; D018117:Thogotovirus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000546:Amanitins; D000956:Antigens, Viral; D009698:Nucleoproteins; D003609:Dactinomycin; D004257:DNA Polymerase II