Characterization of forced suspension culture of spontaneously transformed mouse fibroblasts (B-6).
Mouse fibroblasts (B-6) were cultured on agar-coated dishes. After cells grew for 2-3 generations relatively rapidly in suspension, they began to grow very slowly (stationary phase). Electron microscopic studies showed that cells in a stationary phase developed intracellular organella: membranous structures (endoplasmic reticulum and Golgi apparatus) became manifest and the number of mitochondria increased. The specific activities of succiniccytochrome c reductase and 5'-nucleotidase were three and five times higher, respectively, than those of cells on the dish.
Matsuhisa
T
T
Department of Cell Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
Takemura
K
K
D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002461:Cell Line, Transformed; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
D013384:Succinate Cytochrome c Oxidoreductase; D015720:5'-Nucleotidase