Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody.
Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus, Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 15 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.
Iacono-Connors
L C
LC
Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
Smith
J F
JF
Ksiazek
T G
TG
Kelley
C L
CL
Schmaljohn
C S
CS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D004669:Encephalitis Viruses, Tick-Borne; D000939:Epitopes; D005260:Female; D005416:Flavivirus / Q000276:immunology; D007116:Immunization, Passive; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D014759:Viral Envelope Proteins / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; C100315:NS1 protein, Flavivirus; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C074932:prM protein, Flavivirus; C065696:glycoprotein E, Flavivirus