title: Motor dysfunction in a photothrombotic focal ischaemia model.
abstract: The study of behavioural deficits resulting from cerebral infarction in animal models of stroke has in the past taken second place to histological assessment. This is particularly true of the photothrombotic lesion model. Most tests currently used to measure motor deficits use a scoring system to quantify parameters such as beam walking. The present study set out to characterise a simple and objective assessment for motor impairment in the photothrombotic cortical lesion model. Rats were assessed on a number of motor function tests, i.e. gross locomotor activity, rotarod, and grip strength. After the establishment of stable baselines, cortical photothrombotic lesions were induced, after which the animals were re-tested for a further 18 days. The presence of cortical photothrombotic lesions significantly imparied the rats' performance on the rotarod and grip-strength tests. The deficit observed with the grip-strength task appeared 24 h postsurgery, but was much reduced by day 18 postsurgery. The rotarod test revealed an effect that took longer to establish, but which was more persistent. Gross locomotor activity was not affected. These data suggest that bilateral photothrombotic lesions of the prefrontal cortex produce deficits that can be detected by rotarod and grip-strength tasks.
authors.affiliation: Department of Neurology Research, SmithKline Beecham Pharmaceuticals, Harlow, UK.
authors.forename: N I
authors.initials: NI
authors.lastname: Wood
authors.forename: B V
authors.initials: BV
authors.lastname: Sopesen
authors.forename: J C
authors.initials: JC
authors.lastname: Roberts
authors.forename: P
authors.initials: P
authors.lastname: Pambakian
authors.forename: A L
authors.initials: AL
authors.lastname: Rothaul
authors.forename: A J
authors.initials: AJ
authors.lastname: Hunter
authors.forename: T C
authors.initials: TC
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology / Q000523:psychology*; D005336:Fiber Optic Technology; D018737:Hand Strength / Q000502:physiology; D008027:Light; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009069:Movement Disorders / Q000209:etiology / Q000523:psychology*; D004856:Postural Balance / Q000502:physiology; D011597:Psychomotor Performance / Q000502:physiology; D051381:Rats; D013927:Thrombosis / Q000473:pathology / Q000523:psychology*; D013997:Time Factors