title: CD69 expression on primitive progenitor cells and hematopoietic malignancies.
abstract: CD69 is an early activation antigen of peripheral blood lymphocytes and is constitutively expressed on a wide variety of bone marrow-derived cells. To further characterize the distribution and understand the potential biological role of the molecule in normal and malignant hematopoiesis, we used a novel high affinity anti-CD69 mAb (UN6) and analyzed hematopoietic progenitor cells together with a panel of myeloid and lymphoid malignancies. We report that mobilized peripheral blood CD34+ cells display detectable levels of CD69 and that the density of membrane expression correlates with the immature phenotype CD34bright Thy-1bright cells. Furthermore, during cytokine-induced differentiation, the expression of CD69 is moderately down-regulated. Analysis of hematopoietic malignancies revealed that CD69 expression correlates with the immature myeloid phenotype. Taken together these data suggest a role of CD69 during the early phase of hematopoiesis and in the leukemic transformation.
authors.affiliation: Department of Experimental and Clinical Medicine, Facoltà di Medicina e Chirurgia, Catanzaro, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Tassone
authors.forename: M C
authors.initials: MC
authors.lastname: Turco
authors.forename: F
authors.initials: F
authors.lastname: Tuccillo
authors.forename: P
authors.initials: P
authors.lastname: Bonelli
authors.forename: G
authors.initials: G
authors.lastname: Morrone
authors.forename: L
authors.initials: L
authors.lastname: Cecco
authors.forename: M
authors.initials: M
authors.lastname: Cerra
authors.forename: H
authors.initials: H
authors.lastname: Bond
authors.forename: M
authors.initials: M
authors.lastname: Di Nicola
authors.forename: A M
authors.initials: AM
authors.lastname: Gianni
authors.forename: S
authors.initials: S
authors.lastname: Venuta
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D018952:Antigens, CD34 / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis*; D002648:Child; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D037181:Lectins, C-Type; D007938:Leukemia / Q000276:immunology*; D008214:Lymphocytes / Q000378:metabolism; D008875:Middle Aged; D063268:Sialic Acid Binding Ig-like Lectin 3; D018800:Thy-1 Antigens / Q000096:biosynthesis; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D063268:Sialic Acid Binding Ig-like Lectin 3; D018800:Thy-1 Antigens; D000099192:CD69 Antigens; C571163:CD33 protein, human