Change in the expression of C-type natriuretic peptide and its receptor, B-type natriuretic peptide receptor, during dedifferentiation of chondrocytes into fibroblast-like cells.
Chondrocytes derived from rat xiphoid cartilage dedifferentiated into fibroblast-like cells as the number of passages of the cells in culture increased. During in vitro dedifferentiation the growth of the cells was markedly suppressed. We had proposed previously that C-type natriuretic peptide (CNP) might be a potent antimitogenic factor for chondrocytes, and TGF-beta 1 induced a marked increase in CNP secretion of chondrocytes. Therefore, we investigated the expression of CNP, B-type natriuretic peptide receptor (NPR-B or GC-B), and TGF-beta 1 in this process. Radioimmunoassay and RNase protection analyses revealed passage-associated increase in CNP-like immunoreactivity and in levels of NPR-B mRNA, respectively. Northern blot analyses showed that the level of TGF-beta 1 mRNA decreased with increasing passage number. These results suggest that the expression of CNP and NPR-B might be involved in in vitro dedifferentiation of chondrocytes and TGF-beta 1 does not affect the increasing level of CNP during in vitro dedifferentiation.
Hagiwara
H
H
Research Center for Experimental Biology, Tokyo Institute of Technology, Yokohama.
Inoue
A
A
Furuya
M
M
Tanaka
S
S
Hirose
S
S
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006162:Guanylate Cyclase / Q000235:genetics*; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000502:physiology
D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D020098:Natriuretic Peptide, C-Type; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C