title: Modified India ink preparation for Cryptococcus neoformans in cerebrospinal fluid specimens.
abstract: A novel modified India ink technique for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid specimens is described. It employs 2% chromium mercury and India ink. This technique allows a clear identification of some external and internal structures of the organism. Three layers from the outer capsule that have previously been discerned only by electron microscopy are distinguished.
authors.affiliation: Universidad Nacional Mayor de San Marcos, Lima, Peru.
authors.forename: R
authors.initials: R
authors.lastname: Zerpa
authors.forename: L
authors.initials: L
authors.lastname: Huicho
authors.forename: A
authors.initials: A
authors.lastname: Guillén
mesh_terms: D002244:Carbon; D004396:Coloring Agents; D003453:Cryptococcosis / Q000134:cerebrospinal fluid*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D006801:Humans
chemical_list: D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon