Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay.
Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method.
Simonsson
P
P
Department of Clinical Chemistry, Hospital of Engelholm, Sweden.
Lindberg
S
S
Alling
C
C
D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000097:blood; D000437:Alcoholism / Q000097:blood / Q000175:diagnosis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D012016:Reference Values; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
D014168:Transferrin; C056759:carbohydrate-deficient transferrin