title: Interleukin-10 inhibition of interleukin-6 in human amniochorionic membrane: transcriptional regulation.
abstract: OBJECTIVE
  Our purpose was to study the regulatory effects of recombinant interleukin-10 on interleukin-6 messenger ribonucleic acid and protein production by human fetal membranes.
  
  
  METHODS
  Amniochorionic membranes were collected from women undergoing elective cesarean section. Membranes were maintained in an organ explant system and stimulated with media containing lipopolysaccharide (50 ng/ml) and various amounts of recombinant interleukin-10 (10, 50, 100 ng/ml). Experiments were conducted in a dose- and time-dependent manner. Transcription and translation of interleukin-6 were monitored with quantitative reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Interleukin-10 stimulation of amniochorionic membranes in culture produced a dose-dependent decrease in the production of interleukin-6 messenger ribonucleic acid and protein. Quantitative polymerase chain reaction was used to document a decrease in interleukin-6 messenger ribonucleic acid, which paralleled the decrease in peptide levels as detected with enzyme-linked immunosorbent assay. The interleukin-10 effect was present only when tissue was concurrently stimulated with lipopolysaccharide. Interleukin-10 inhibition could not be produced in the absence of lipopolysaccharide stimulation.
  
  
  CONCLUSIONS
  Addition of interleukin-10 to culture media leads to transcriptional regulation of interleukin-6, which results in decreased production of both messenger ribonucleic acid and protein by human amniochorionic membranes. The decrease in interleukin-6 is a dose-dependent effect of interleukin-10. This finding may have important implications with respect to a possible role for interleukin-10 or an interleukin-10 stimulatory factor in the management of preterm labor associated with the presence of inflammatory cytokines.
authors.affiliation: Maternal-Fetal Group, Women's Hospital, Centennial Medical Center, Nashville, TN 37203, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Fortunato
authors.forename: R
authors.initials: R
authors.lastname: Menon
authors.forename: K F
authors.initials: KF
authors.lastname: Swan
authors.forename: S J
authors.initials: SJ
authors.lastname: Lombardi
mesh_terms: D000650:Amnion / Q000187:drug effects / Q000378:metabolism*; D002823:Chorion / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D013997:Time Factors; D014158:Transcription, Genetic; D000093242:Interleukin-6 Inhibitors
chemical_list: D016753:Interleukin-10; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000093242:Interleukin-6 Inhibitors