title: Secretoneurin-induced in vitro chemotaxis of human monocytes is inhibited by pertussis toxin and an inhibitor of protein kinase C.
abstract: The sensory neuropeptide secretoneurin (SN) triggers chemotactic migration of monocytes. We have investigated the possibility that SN, like other chemoattractants such as formyl-Met-Leu-Phe and chemokines, might stimulate migration of monocytes by G protein and protein kinase C (PKC) activation and induce Ca2+i release. We report that preincubation of monocytes with pertussis toxin inhibited SN chemotaxis. Staurosporine, an inhibitor of PKC, significantly decreased SN-induced chemotaxis of monocytes, suggesting that PKC may be involved in the signaling. Tyrphostin-23, which inhibits tyrosin kinase, did not affect SN-induced chemotaxis of monocytes. This suggests that SN uses a signaling mechanism that is coupled to pertussis toxin-sensitive G proteins. Involvement of phospholipase C beta as a result of PKC activation is suggested by a SN-induced increase of intracellular Ca2+ concentration in monocytes.
authors.affiliation: Department of Internal Medicine, Medical Faculty, University of Innsbruck, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Schratzberger
authors.forename: E
authors.initials: E
authors.lastname: Wöll
authors.forename: N
authors.initials: N
authors.lastname: Reinisch
authors.forename: C M
authors.initials: CM
authors.lastname: Kähler
authors.forename: C J
authors.initials: CJ
authors.lastname: Wiedermann
mesh_terms: D000814:Aniline Compounds; D002118:Calcium / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology; D037342:Pertussis Toxin; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D053381:Secretogranin II; D020032:Tyrphostins; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D002396:Catechols; D004791:Enzyme Inhibitors; D009479:Neuropeptides; D009570:Nitriles; D053381:Secretogranin II; D020032:Tyrphostins; D010566:Virulence Factors, Bordetella; D014966:Xanthenes; C081159:secretoneurin; C059715:Fluo-3; D009240:N-Formylmethionine Leucyl-Phenylalanine; D037342:Pertussis Toxin; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; C060641:tyrphostin A23; D002118:Calcium