title: Induction of intercellular adhesion molecule-1 in human nasal epithelial cells during respiratory syncytial virus infection.
abstract: The effects of infection with respiratory syncytial virus (RSV) on expression of intercellular adhesion molecule (ICAM)-1 was determined in vitro in nasal epithelial cell cultures. Functional consequences of changes in ICAM-1 expression were assessed by measuring adhesion of a human leukaemic T-cell line to RSV-infected epithelial cells. Also, adhesion of phytohaemagglutinin-activated tonsillar lymphocytes (TL) to RSV-infected epithelial cells caused a significant increase in interleukin (IL)-4 or IL-5 production. Release of these cytokines was adhesion dependent as non-adherent TL produced significantly less IL-4 or IL-5. However, no significant difference was observed for IL-2 or interferon-gamma (IFN-gamma) production. These observations suggest that RSV-infected epithelial cells may induce T-helper type-2 (Th2)-like cytokines by mucosal lymphocytes during mucosal infection in vivo.
authors.affiliation: Department of Otolaryngology, Akita University School of Medicine, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Matsuzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: N
authors.initials: N
authors.lastname: Sarashina
authors.forename: E
authors.initials: E
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Togawa
authors.forename: I
authors.initials: I
authors.lastname: Saito
mesh_terms: D000328:Adult; D001483:Base Sequence; D018929:Cell Culture Techniques; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D018928:Immunity, Mucosal; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D010835:Phytohemagglutinins / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*
chemical_list: D016207:Cytokines; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1