title: Accelerated myelinogenesis by dietary lipids in rat brain.
abstract: Our previous work showed an early development of behavioral reflexes in rats whose mothers had been fed, during pregnancy and lactation, a lipid fraction extracted from yeast grown on n-alkanes (which contain 50% odd-chain fatty acids) in comparison with controls fed a margarine diet. To clarify whether the observed changes might be linked to an early myelination, we have investigated mRNAs involved in myelin synthesis in the brains of offspring at 5 days of age by northern blot and in situ hybridization. Northern blot analysis showed that proteolipid protein (PLP) and myelin oligodendrocyte glycoprotein (MOG) mRNAs were higher in animals on the lipid diet compared with controls. In situ hybridization with probes specific for PLP, myelin basic protein, and MOG mRNA showed significantly higher numbers of positive cells in test animals compared with controls in all brain regions. This study shows an acceleration of myelinogenesis induced by dietary lipids. These data can give a new insight in the therapeutical approaches involved to promote repair in demyelinating diseases.
authors.affiliation: Cellular Biology-Istituto Superiore di Sanità, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Salvati
authors.forename: M
authors.initials: M
authors.lastname: Sanchez
authors.forename: L M
authors.initials: LM
authors.lastname: Campeggi
authors.forename: G
authors.initials: G
authors.lastname: Suchanek
authors.forename: H
authors.initials: H
authors.lastname: Breitschop
authors.forename: H
authors.initials: H
authors.lastname: Lassmann
mesh_terms: D000473:Alkanes; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002175:Candida; D002540:Cerebral Cortex / Q000254:growth & development; D003314:Corn Oil; D015342:DNA Probes; D004041:Dietary Fats; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007774:Lactation; D008383:Margarine; D008526:Medulla Oblongata / Q000254:growth & development; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis*; D009186:Myelin Sheath / Q000502:physiology*; D018992:Myelin-Associated Glycoprotein / Q000096:biosynthesis*; D063308:Myelin-Oligodendrocyte Glycoprotein; D009836:Oligodendroglia / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D000473:Alkanes; D015342:DNA Probes; D004041:Dietary Fats; C571328:Mog protein, rat; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D012333:RNA, Messenger; D003314:Corn Oil; D008383:Margarine