title: Comparative effects of platelet-derived growth factor-BB and insulin-like growth factor-I, individually and in combination, on periodontal regeneration in Macaca fascicularis.
abstract: Platelet-derived growth factor (PDGF) and insulin-like growth factor I (IGF-I) in combination have previously been shown to enhance periodontal regeneration. The objective of this study was to further characterize the biological effects of this combination of growth factors in non-human primates and compare the effects to those of each growth factor individually. Ligature-induced periodontitis was initiated in 10 cynomolgus monkeys. After periodontal lesions were established, surgery was performed, and either a methylcellulose gel vehicle or vehicle containing 10 micrograms each of either PDGF-BB, IGF-I or both PDGF-BB and IGF-I was applied to exposed root surfaces. Biopsies were taken 4 and 12 wk after treatment and the extent of periodontal regeneration was assessed by histomorphometry. At both 4 and 12 wk vehicle-treated lesions generally revealed minimal osseous defect fill (ODF) (8.5 +/- 2.1% and 14.5 +/- 5.7%, respectively) and new attachment (NA) (34.1 +/- 5.2% and 26.6 +/- 10.5%, respectively). IGF-I treatment did not significantly alter healing compared to vehicle in any parameter at both 4 and 12 wk. PDGF-BB-treated sites exhibited significant (p < 0.05) regeneration of NA (69.6 + 12.0%) at 12 wk; trends for PDGF-BB treatment effect were also observed in other parameters at 4 and 12 wk, although these increases were not statistically significant. Treatment with PDGF-BB/IGF-I resulted in 21.6 +/- 5.1% and 42.5 +/- 8.3% ODF at 4 and 12 wk, respectively, and 64.1 +/- 7.7% and 74.6 +/- 7.4% NA at 4 and 12 wk, respectively (all significantly greater than vehicle, p < 0.05). The results from this study demonstrated that: 1) IGF-I alone at the dose tested did not significantly alter periodontal wound healing; 2) PDGF-BB alone significantly stimulated NA, with trends of effect on other parameters; and 3) the PDGF-BB/IGF-I combination resulted in significant increases in NA and ODF above vehicle at both 4 and 12 wk.
authors.affiliation: Department of Periodontology, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: W V
authors.initials: WV
authors.lastname: Giannobile
authors.forename: R A
authors.initials: RA
authors.lastname: Hernandez
authors.forename: R D
authors.initials: RD
authors.lastname: Finkelman
authors.forename: S
authors.initials: S
authors.lastname: Ryan
authors.forename: C P
authors.initials: CP
authors.lastname: Kiritsy
authors.forename: M
authors.initials: M
authors.lastname: D'Andrea
authors.forename: S E
authors.initials: SE
authors.lastname: Lynch
mesh_terms: D000539:Alveolar Process / Q000187:drug effects* / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D000077214:Becaplermin; D001861:Bone Regeneration / Q000187:drug effects; D004338:Drug Combinations; D004357:Drug Synergism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008252:Macaca fascicularis; D010510:Periodontal Diseases / Q000601:surgery; D010513:Periodontal Ligament / Q000187:drug effects* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects
chemical_list: D004338:Drug Combinations; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I