Synteny mapping in river buffalo.
The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP-YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology between cattle and river buffalo, comparative mapping predicts the localization of these syntenic groups on river buffalo Chromosomes (Chrs) :BBU7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been previously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.
El Nahas
S M
SM
Department of Cell Biology, National Research Center, Dokki, Cairo, Egypt.
Oraby
H A
HA
de Hondt
H A
HA
Medhat
A M
AM
Zahran
M M
MM
Mahfouz
E R
ER
Karim
A M
AM
D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D008214:Lymphocytes; D015183:Restriction Mapping