title: A monoclonal antibody against human lipoprotein lipase inhibiting heparin binding without affecting catalytic activity.
abstract: A fragment of the human lipoprotein lipase (LPL) cDNA (405 bp, 5' terminal end) was cloned in an expression vector to produce a approximately 17 kDa fusion peptide and was used as antigen to produce a high titre anti-LPL monoclonal antibody (10C3 MAb). This antibody reacts with both native and denatured forms of LPL from different tissue and animal sources. Competition studies with heparin indicate that 10C3 MAb is specific for an epitope at a heparin binding site. The antibody does not inhibit LPL enzyme activity, indicating that the antigenic epitope is not situated within or in the proximity of the LPL catalytic region. With these characteristics, 10C3 MAb should prove to be a useful immunochemical tool in clinical as well as in fundamental investigations on the metabolism of triglyceride-rich lipoproteins and in studies on the functional anatomy of LPL.
authors.affiliation: Lipid Research Centre, CHUL Research Centre, Ste-Foy, QC, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Cadelis
authors.forename: P
authors.initials: P
authors.lastname: Julien
authors.forename: J P
authors.initials: JP
authors.lastname: Valet
authors.forename: Y
authors.initials: Y
authors.lastname: Deshaies
authors.forename: M R
authors.initials: MR
authors.lastname: Ven Murthy
authors.forename: P J
authors.initials: PJ
authors.lastname: Lupien
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D008071:Lipoprotein Lipase / Q000276:immunology*; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D006493:Heparin; D008071:Lipoprotein Lipase