Synthesis and inhibitory properties of a thiomethylmercuric sialic acid with application to the X-ray structure determination of 9-O-acetylsialic acid esterase from influenza C virus.
2-alpha-Thiomethylmercuryl 9-acetamido-9-deoxy-sialoside was prepared and found to inhibit the 9-O-acetylsialic acid esterase from influenza C virus in a competitive manner with a Ki of 4.2 +/- 0.5 mM. The inhibitor is being used in the X-ray determination of the crystal structure of the esterase.
Fitz
W
W
Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA.
Rosenthal
P B
PB
Wong
C H
CH
D000115:Acetylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D018360:Crystallography, X-Ray; D009982:Gammainfluenzavirus / Q000201:enzymology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D012794:Sialic Acids / Q000138:chemical synthesis / Q000494:pharmacology*
D012794:Sialic Acids; D002265:Carboxylic Ester Hydrolases; D000115:Acetylesterase; C046298:sialate O-acetylesterase