title: Lethality, hemagglutination and adhesion of Escherichia coli strains (serotype 01) isolated in Morocco from chickens with colibacillosis.
abstract: Six strains of Escherichia coli, isolated from chickens with colibacillosis and belonging to serotype 01, were studied. All of them were lethal for 1-day-old chicks and were strongly adherent in vitro. Hemagglutination tests showed a strong reaction to horse and chicken erythrocytes. This reaction was inhibited by the addition of 5% D-mannose and also by overnight incubation at 18 C. When the strains were heated at 65 C or at 85 C for 1 hr, agglutination of horse and chicken erythrocytes was conserved only at 65 C. Purification of the subunit of fimbriae by several cycles of solubilization-crystallization using MgCl2 gave good results. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the molecular weight for one strain was about 18.4 kD.
authors.affiliation: Département de Pathologie Aviaire, Institut Agronomique et Vétérinaire Hassan II, Rabat-Instituts, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Amara
authors.forename: S E
authors.initials: SE
authors.lastname: Had
authors.forename: T
authors.initials: T
authors.lastname: Jirrari
authors.forename: K
authors.initials: K
authors.lastname: Bouzoubaa
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002645:Chickens / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D006386:Hemagglutination Tests / Q000662:veterinary; D009018:Morocco; D011201:Poultry Diseases / Q000382:microbiology*