Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.
Neurobasal is a bicarbonate-buffered medium optimized for the growth of embryonic rat hippocampal neurons at pH 7.3 in 5% CO2. Neurons die within hours in this or in other 26 mM bicarbonate buffers when transferred to ambient CO2 (0.2%). Death is associated with a rapid rise in medium pH to 8.1. A new CO2-independent modification of Neurobasal (Hibernate E), when supplemented with B27, can maintain neuron viability for at least 2 days in ambient CO2. This same medium can also be used to store viable brain tissue for up to a month with refrigeration. These advances should facilitate studies of neuron physiology outside the incubator as well as storing and transporting neuronal tissue.
Brewer
G J
GJ
Southern Illinois University School of Medicine, Springfield 62794-1220, USA.
Price
P J
PJ
D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D051381:Rats
D002245:Carbon Dioxide