Thapsigargin enhances carotid body chemosensory discharge in response to hypoxia in zero [Ca2+]e: evidence for intracellular Ca2+ release.
To test the hypothesis that Ca2+ is released from intracellular store in the carotid body glomus cells during hypoxia, we stimultaneously measured chemosensory discharge and tissue PO2 of perfused-superfused cat carotid body before and during flow interruption in the presence and absence of extracellular [Ca2+] with and without thapsigargin (1-10 microM). Ca(2+)-free solution increased the latency of sensory response, and decreased the rate of rise and peak activity but thapsigargin significantly influenced these responses, without affecting oxygen consumption. Since thapsigargin depletes the intracellular Ca2+ store, and since Ca2+ is needed for the sensory discharge, these results suggest that intracellular release and influx of Ca2+ occur during hypoxia.
Lahiri
S
S
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6085, USA. LAHIRI@A1.MSCF.UPENN.EDU
Osanai
S
S
Buerk
D G
DG
Mokashi
A
A
Chugh
D K
DK
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002344:Carotid Body / Q000187:drug effects* / Q000503:physiopathology*; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects* / Q000503:physiopathology*; D005110:Extracellular Space / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology*; D007425:Intracellular Membranes / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D019284:Thapsigargin / Q000494:pharmacology*
D019284:Thapsigargin; D000252:Calcium-Transporting ATPases; D002118:Calcium