Evaluation of cyclic AMP accumulation in EBV-transformed human B-lymphocytes: effects of dopamine agonists, isoproterenol, prostaglandin E1, cholera toxin, forskolin, and phorbol 12-myristate-13 acetate.
1. Phorbol 12-myristate-13-acetate (PMA), a protein kinase C activator, elevated cyclic AMP accumulation in EBV-transformed human B-lymphocytes, and potentiated isoproterenol-, prostaglandin- (PGE1), cholera toxin-, and forskolin-stimulated cyclic AMP accumulation. 2. The dopamine D1 receptor agonist, SKF38393 (10(-7) to 10(-5) MH, had no effect on cyclic AMP accumulation in transformed human B-lymphocytes. 3. The dopamine D2 receptor agonist, quinpirole (10(-7) to 10(-5) MH did not inhibit cyclic AMP accumulation even when cyclic AMP accumulation was maximized by the addition of PMA and forskolin. 4. These data suggest that dopamine D1- and D2-receptor coupling to a cyclic AMP generating system is not present at detectable levels in transformed human B-lymphocytes.
Natsukari
N
N
National Institute of Mental Health, Neuroscience Center at Saint Elizabeth Hospital, Neuropsychiatry Branch, Washington, D.C., USA.
Kulaga
H
H
Baker
I
I
Wyatt
R J
RJ
Masserano
J M
JM
D000527:Alprostadil / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects*; D002772:Cholera Toxin / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology
D018491:Dopamine Agonists; D010703:Phorbol Esters; D005576:Colforsin; C008874:phorbol-12,13-diacetate; D002772:Cholera Toxin; D000242:Cyclic AMP; D000527:Alprostadil; D007545:Isoproterenol