Efficient gene transfer to EGF receptor overexpressing cancer cells by means of EGF-labeled cationic liposomes.
Epidermal growth factor (EGF)-labeled cationic liposomes (EGF-liposomes) were prepared for efficient gene transfer vector to EGF receptor expressing cells. Transfection activity of EGF-liposomes associating plasmid PGV-C which encodes luciferase showed a 2-fold increase in EGF receptor expressing cells, HEC-1-A, compared to that of EGF-non-labeled liposomes (N-liposomes). In EGF receptor deficient HRA cells, however, both EGF- and N-liposomes exhibited low transfection efficiency and no difference was observed between them. Furthermore, by the addition of anti EGF receptor antibody, transfection efficiency of EGF-liposomes was suppressed, suggesting EGF receptor-mediated endocytosis of EGF-liposomes. Transfection activity of EGF-liposomes was strongly dependent on the concentrations of fusogenic lipid, dioleoylphospha-tidylethanolamine in liposomes. By X-gal staining 6-8% of GCH-1(m) cells which also had EGF receptor expressed beta-galactosidase activity following the transfection by EGF-liposomes associating pSV-beta-galactosidase. These findings indicate that EGF-liposomes could be a preferable vector for EGF-receptor expressing cells.
Kikuchi
A
A
Department of Obstetrics and Gynecology, Niiugata University School of Medicine, Japan.
Sugaya
S
S
Ueda
H
H
Tanaka
K
K
Aramaki
Y
Y
Hara
T
T
Arima
H
H
Tsuchiya
S
S
Fuwa
T
T
D015060:1,2-Dipalmitoylphosphatidylcholine; D002412:Cations; D002784:Cholesterol / Q000031:analogs & derivatives; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics*; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D010714:Phosphatidylethanolamines; D014407:Tumor Cells, Cultured
D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; D015060:1,2-Dipalmitoylphosphatidylcholine; D004815:Epidermal Growth Factor; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol; D066246:ErbB Receptors