Use of the two-hybrid system and random sonicated DNA to identify the interaction domain of a protein.
Stagljar
I
I
Institut Für Molekularbiologie, Universität Zürich, Switzerland.
Bourquin
J P
JP
Schaffner
W
W
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012641:Selection, Genetic; D013010:Sonication; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; D004271:DNA, Fungal; D005656:Fungal Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53