Structures and contribution to the antigenicity of oligosaccharides of Japanese cedar (Cryptomeria japonica) pollen allergen Cry j I: relationship between the structures and antigenic epitopes of plant N-linked complex-type glycans.
The oligosaccharide structures of Cry j I, a major allergenic glycoprotein of Cryptomeria japonica (Japanese cedar, sugi), were analysed by 400 MHz 1H-NMR and two-dimensional sugar mapping analyses. The four major fractions comprised a series of biantennary complex type N-linked oligosaccharides that share a fucose/xylose-containing core and glucosamine branches including a novel structure with a nongalactosylated fucosylglucosamine branch. Rabbit polyclonal anti-Cry j I IgG antibodies cross-reacted with three different plant glycoproteins having the same or shorter N-linked oligosaccharides as Cry j I. ELISA and ELISA inhibition studies with intact glycoproteins, glycopeptides and peptides indicated that both anti-Cry j I IgGs and anti-Sophora japonica bark lectin II (B-SJA-II) IgGs included oligosaccharide-specific antibodies with different specificities, and that the epitopic structures against anti-Cry j I IgGs include a branch containing alpha 1-6 linked fucose and a core containing fucose/xylose, while those against anti-B-SJA-II IgGs include nonreducing terminal mannose residues. The cross-reactivities of human allergic sera to miraculin and Clerodendron Trichotomum lectin (CTA) were low, and inhibition studies suggested that the oligosaccharides on Cry j I contribute little or only conformationally to the reactivity of specific IgE antibodies.
Ogawa
H
H
Department of Chemistry, Faculty of Science, Ochanomizu University, Tokyo, Japan.
Hijikata
A
A
Amano
M
M
Kojima
K
K
Fukushima
H
H
Ishizuka
I
I
Kurihara
Y
Y
Matsumoto
I
I
D000485:Allergens / Q000737:chemistry; D052179:Antigens, Plant; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology; D011058:Pollen / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D014197:Trees / Q000737:chemistry*
D000485:Allergens; D052179:Antigens, Plant; D002241:Carbohydrates; C065290:Cry j I protein, Cryptomeria japonica; D000939:Epitopes; D009844:Oligosaccharides; D010940:Plant Proteins; D011134:Polysaccharides