Characterization of natriuretic peptide receptors in the rat parotid.
Competition studies between 125I-ANP and ANP (atrial natriuretic peptide) or the ring-deleted analog C-ANF4-23 (C-ANF) revealed the presence of the A-type natriuretic peptide receptor (GC-A receptor) and the ANP-clearance (ANP-C) receptor in the rat parotid membrane. The ratio of the GC-A and ANP-C receptors was about 3 to 1. Enhancement of the cGMP level by the C-type natriuretic peptide (CNP) revealed the presence of the B-type natriuretic peptide receptor (GC-B receptor) in the rat parotid gland. Based on the binding studies with ANP and C-ANF and cGMP analysis by ANP and CNP, the natriuretic peptide receptors in the rat parotid gland were demonstrated to have a rank order on the amount of GC-A receptor > GC-B receptor > ANP-C receptor.
Nashida
T
T
Department of Oral Biochemistry, Nippon Dental University School of Dentistry at Niigata, Japan.
Imai
A
A
Shimomura
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D007700:Kinetics; D008297:Male; D020098:Natriuretic Peptide, C-Type; D010306:Parotid Gland / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000737:chemistry* / Q000378:metabolism
D011506:Proteins; D012333:RNA, Messenger; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP