Determination of pregastric lipase specificity in young ruminants.
The combined hydrolysis of a chiral triglyceride clearly defined on these three positions and of its racemic equivalent reveals all sorts of lipase specificities: regioselectivity, typoselectivity and stereospecificity. This article describes a study of the pregastric lipases of young ruminants (kid, calf, lamb), whose specificity is still somewhat ambiguous. The use of a chiral triglyceride in which position sn 1 was occupied by a short-chain fatty acid showed that these lipases had dual specificity, i.e. a combination of typoselectivity with respect to short-chain fatty acids and sn3 stereospecificity. A comparison between the performance of the lipase of young animals and the fatty acid composition and distribution in the mother's milk suggests that the degree of dual specificity in these enzymes is related to the characteristics of the mother's milk.
Villeneuve
P
P
Laboratoire de Lipotechnie, CIRAD-CP, Montpellier, France.
Pina
M
M
Graille
J
J
D000818:Animals; D002417:Cattle; D004064:Digestive System / Q000201:enzymology; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006041:Goats; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008892:Milk / Q000378:metabolism; D012756:Sheep; D013237:Stereoisomerism; D013379:Substrate Specificity; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism
D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase