Effect of 3-deoxyglucosone on the activities of enzymes responsible for glucose metabolism in mouse liver.
Crude extracts containing the enzymes obtained from mouse liver were incubated with 3-deoxyglucosone (3-DG), and then subjected to assay of the activities of enzymes responsible for glucose metabolism. Hexokinase and glucose-6-phosphate dehydrogenase activities were decreased by 3-DG and hexokinase activity was strongly inhibited time and concentration dependently, while glucokinase, glucose-6-phosphatase, and phosphofructokinase activities were scarcely affected. These results suggest that 3-DG inhibits the intake of glucose in the liver and a connection with development of diabetes.
Kiho
T
T
Department of Hygienic Chemistry, Gifu Pharmaceutical University, Japan.
Asahi
T
T
Usui
S
S
Matsunaga
T
T
Ukai
S
S
D000818:Animals; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice
C060458:3-deoxyglucose; D003847:Deoxyglucose; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D005947:Glucose