Comparison of camelpox viruses isolated in Dubai.
Between October 1993 and March 1994, outbreaks of pox-like exanthemas were observed in several camel raising farms in Dubai. Scabs from twenty camels with either local or generalized lesions were examined, seven of them had previously been vaccinated with a modified live camelpox virus vaccine. Inspection of scabs by electron microscopy confirmed an infection with orthopox viruses (OPV) in 10 animals and with parapox virus in one camel. Investigation of the scabs by polymerase chain reaction and dot blot assay revealed the presence of OPV in 15 or 13 samples, respectively. OPV could be isolated in cell culture in 14 cases. Restriction enzyme profiles characterized all isolates as camelpox virus. Their DNA patterns were virtually identical displaying only slight variations in the terminal fragments. In contrast, the vaccine strain showed a distinct restriction enzyme profile, indicating that it was not involved in the infections.
Pfeffer
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Institute for Medical Microbiology, infectious and Epidemic Diseases, Ludwig-Maximilians University, Veterinaerstr, Germany.
Meyer
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H
Wernery
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U
Kaaden
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OR
D000818:Animals; D002162:Camelus / Q000821:virology*; D018147:Chordopoxvirinae / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction / Q000379:methods*; D011213:Poxviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D015183:Restriction Mapping; D014479:United Arab Emirates
D015342:DNA Probes; D004279:DNA, Viral