Suppression of mitogen-induced lymphocyte proliferation by syringomycin-E.
Syringomycin E (SR-E) is a low molecular weight bacterial lipodepsipeptide with antifungal properties. Owing to immunosuppressive activities of such compounds as cyclosporine, FK506 and rapamycin, we studied the effect of SR-E on proliferation of human blood lymphocytes in vitro. SR-E, by itself, had no effect but the mitogen-induced lymphocyte proliferation was significantly suppressed. The suppressive effect was more pronounced with pokeweed mitogen (PWM) as compared to phytohemagglutinin (PHA) or monoclonal antibody to CD3 (anti-CD3). Since these mitogens induce cellular immunity (T cell-dependent), SR-E may potentially be a novel immunosuppressive compound.
Singh
V K
VK
Immunology Laboratory, MSRB-I, University of Michigan, Ann Arbor 48109-0656, USA.
Takemoto
J Y
JY
D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000494:pharmacology*; D017252:CD3 Complex / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D017252:CD3 Complex; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; C032677:syringomycin