Detection of immunoglobulins on bacterial surface by laser flow cytometry: analysis between Haemophilus influenzae type b and Vibrio cholerae O1 of healthy mother-full term newborn sera.
The identification of human IgG immunoglobulins on the surface of Vibrio cholerae O1, and Haemophilus influenzae type b microorganisms was assessed via a flow cytometric technique. A group of 31 healthy mother-full term newborn duo sera from a non-endemic cholera area was assayed. The sera of mothers and full-term newborns against both microorganisms were compared. The mean fluorescent intensity of the samples was not different at the 0.05 significance level by paired t-test. On the other hand, the immunoglobulins of newborn and mothers for V. cholerae O1 was notably lower when compared with H. influenzae type b microorganisms (p < 0.05 by paired t-test, t = -5.570 for mothers' sera, and t = -7.496 for the sera of the newborns). These data provide circumstantial evidence that LFC technique would be useful on bacteria-related serology.
Cano-Morales
S
S
Depto, de Pediatría del Hospital General de Durango SSA, México.
Luna-Guerrero
R
R
Mendez-Cuevas
G
G
Alvarado-Aleman
F J
FJ
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood*; D007231:Infant, Newborn / Q000097:blood*; D017879:Isothiocyanates; D007834:Lasers; D009035:Mothers; D014734:Vibrio cholerae / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D017879:Isothiocyanates; C048844:anti-IgG; C037152:isothiocyanic acid