Comparison of different electrophoretic methods for digital typing of the MS32 (D1S8) locus.
Minisatellite variant repeat mapping by polymerase chain reaction (MVR-PCR) is a new and powerful approach for individual identification from human DNA. This method has been successfully applied to the hypervariable human minisatellite MS32 (locus D1S8). In this work, we further investigate this locus using different electrophoretic techniques which include: agarose gels and nonradioactive hybridization, miniaturized polyacrylamide gels, followed by silver staining, and automatic detection of fluorescent labeled alleles. The advantages and disadvantages of each method are described. Also, MVR codes from a Galician population (NW Spain) are provided.
Rodriguez-Calvo
M S
MS
Institute of Legal Medicine, University of Santiago de Compostela, Spain.
Bellas
S
S
Soto
J L
JL
Barros
F
F
Carracedo
A
A
D002874:Chromosome Mapping; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes