title: Detection by PCR of human papillomavirus genotypes in cervical lesions of Senegalese women.
abstract: In order to analyse human papillomavirus (HPV) infection in the Senegalese population, HPV DNA was sought in 65 women with evidence of cervical cytological abnormality and in 72 pregnant women. Ninety-four percent of the patients were positive for HPV DNA as compared to 24% of pregnant women. HPV 16 was detected in cervical smears in 42% of cases, HPV 18 in 39%, HPV 6 in 26%, HPV 11 in 15%, HPV 45 in 10%, HPV 52 in 3%, and HPV 31, HPV 33 and HPV 68 in 1.5%. HPV 16 and HPV 18 were detected in 16% and 7% respectively of pregnant women. HPV DNA of unknown type was detected in 6% of cases, and multiple HPV infections were observed in 28% of cases. Low risk genital HPVs (6/11) were detected in smaller proportions (17%) among high grade squamous intraepithelial lesions (SILs) than the low grade SILs (43%). High risk HPVs (16/18) were detected in high proportions both in low and high grade SIL lesions, though the highest frequency (70%) was observed among patients with high grade lesions. In conclusion, the results confirm that HPV infections are frequent in Senegal and that HPV 18 and 45 are detected in a high proportion of patients in Africa.
authors.affiliation: Institut de Virologie de Tours, Faculté de Pharmacie, CHRU Bretonneau, Tours, France.
authors.forename: M
authors.initials: M
authors.lastname: Chabaud
authors.forename: P
authors.initials: P
authors.lastname: Le Cann
authors.forename: V
authors.initials: V
authors.lastname: Mayelo
authors.forename: D
authors.initials: D
authors.lastname: Leboulleux
authors.forename: A S
authors.initials: AS
authors.lastname: Diallo
authors.forename: N
authors.initials: N
authors.lastname: Enogat
authors.forename: J M
authors.initials: JM
authors.lastname: Afoutou
authors.forename: P
authors.initials: P
authors.lastname: Anthonioz
authors.forename: A M
authors.initials: AM
authors.lastname: Coll-Seck
authors.forename: P
authors.initials: P
authors.lastname: Coursaget
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D012307:Risk Factors; D012675:Senegal; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral