Human immunodeficiency virus type 1 activation after blood transfusion.
BACKGROUND
Anemia and transfusion are predictors of disease progression in AIDS patients. This study was designed to examine the effects of blood transfusion on human immunodeficiency virus type 1 (HIV-1) expression.


METHODS
Assays of plasma viral load were performed before and after transfusion in nine HIV-1-infected patients who required blood transfusion for refractory anemia.


RESULTS
There was a modest rise in plasma HIV-1 p24 antigen and plasma HIV-1 RNA beginning 1 to 2 weeks after the blood transfusion. The mean change in plasma p24 antigen for all patients was 9.3 +/- 5.1 (mean +/- SE) pg per mL at Week 2 after transfusion and 18 +/- 11.1 pg per mL at Week 4. Plasma HIV-1 RNA levels were unchanged immediately after transfusion and exceeded pretransfusion levels with a mean rise of 84 +/- 40 percent (SE) at Week 1, 70 +/- 27 percent at Week 2, and 67 +/- 38 percent at Week 4 (p = 0.006, exact permutation test). There was no increase in spontaneous or interleukin 2-induced lymphocyte proliferation or p24 antigen production by patients' lymphocytes that were examined immediately after blood transfusion.


CONCLUSIONS
The transfusion of blood to persons with advanced HIV-1 infection modestly increases plasma levels of HIV-1. The activation of HIV-1 expression by transfusion may help to explain the accelerated course of HIV-1 disease in recipients of blood transfusion.
Mudido
P M
PM
Department of Medicine, Case Western Reserve University, University Hospitals of Cleveland, Ohio, USA.
Georges
D
D
Dorazio
D
D
Yen-Lieberman
B
B
Bae
S
S
O'Brien
W A
WA
Spritzler
J
J
Lederman
M M
MM
D000740:Anemia / Q000209:etiology / Q000628:therapy*; D015658:HIV Infections / Q000150:complications / Q000628:therapy* / Q000821:virology; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D065227:Transfusion Reaction; D019562:Viral Load; D014775:Virus Activation