Photoaffinity labeling of peripheral benzodiazepine receptors in R-3327 Dunning prostatic tumors.
Photoaffinity labeling of peripheral benzodiazepine receptors (PBZr) in mitochondrial and microsomal fractions from AT-1, H, and G Dunning R-3327 tumor sublines was performed using the photoaffinity ligand 3H-PK 14105. Subsequent sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed specific labeling of a 17 kDa protein in the microsomal fractions from these tumors. In the mitochondrial fractions, however, two bands with the apparent molecular mass of 17 and 18 kDa in AT-1 and H tumors were identified, whereas only the 17 kDa band was present in G tumors. Using another ligand, 3H-flunitrazepam, to photoaffinity label PBZr, proteins with molecular masses of 17, 30, 42, and 48 kDa were identified. These data suggest the possibility of different subclasses of PBZrs in Dunning prostatic tumors.
Alenfall
J
J
Department of Pharmacology, University of Lund, Sweden.
Batra
S
S
D000345:Affinity Labels; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005445:Flunitrazepam; D007546:Isoquinolines; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis*; D013347:Subcellular Fractions / Q000378:metabolism
D000345:Affinity Labels; D007546:Isoquinolines; D011506:Proteins; D011963:Receptors, GABA-A; D005445:Flunitrazepam; C051238:PK 14105