title: Interactions between glycoconjugates from human respiratory airways and Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa binds to different glycoconjugates in vitro. As six other bacteria, it binds to several glycolipids, mainly asialo GM1 and asialo GM2. Asialo GM1 has been reported to exist at the surface of cystic fibrosis cells. The binding of P. aeruginosa to asialo GM1 involves the pili, especially the C-terminal part of pilin that recognizes the GaINAc(beta 1,4) Gal sequence of asialo GM1.P. aeruginosa may also bind to sialylated membrane-bound glycoproteins. Human salivary and respiratory mucins are also recognized by P. aeruginosa. Mucins represent the main components of mucus. The peptide part (apomucin) of this broad family of secreted glycoproteins is encoded by several mucin genes. The apomucins are covered by a large number of carbohydrate chains that can be remarkably different and represent a mosaic of sites for attachment of microorganisms. The binding of P. aeruginosa to mucins involves outer membrane proteins and mucin carbohydrate chains that are structurally different from the carbohydrate recognized by pillin. Airway and salivary mucins secreted by patients suffering from cystic fibrosis (CF) show alterations in their carbohydrate moiety. The increased sulfation of airway mucins seems to correspond to a primary defect. Other abnormalities such as increased sialylation or fucosylation have also been detected. The binding of P. aeruginosa to airway or salivary mucins is increased in CF. However, the precise link between the carbohydrate alterations and the increased binding of P. aeruginosa to CF mucins remains to be elucidated.
authors.affiliation: Unité INSERM No. 377, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Scharfman
authors.forename: E
authors.initials: E
authors.lastname: Van Brussel
authors.forename: N
authors.initials: N
authors.lastname: Houdret
authors.forename: G
authors.initials: G
authors.lastname: Lamblin
authors.forename: P
authors.initials: P
authors.lastname: Roussel
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D003550:Cystic Fibrosis / Q000382:microbiology; D006001:Glycoconjugates / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012137:Respiratory System / Q000378:metabolism / Q000382:microbiology*
chemical_list: D006001:Glycoconjugates; D006017:Glycolipids; D008562:Membrane Glycoproteins; D009077:Mucins