title: Isolation of the ATP-binding human homolog of the arsA component of the bacterial arsenite transporter.
abstract: Arsenite resistance in bacteria is mediated by an efflux pump composed of the arsA and arsB gene products. We have isolated the human homolog of the bacterial arsA (hARSA-I), a member of the ATPase superfamily with no transmembrane domain. Southern and Northern analyses indicated the presence of two cross-hybridizing genes in the human genome and expression of hARSA-I in many tissues. A rabbit antiserum raised against a glutathione-S-transferase (GST)/hARSA-I fusion protein identified two cross-reacting proteins of 37 and 42 kDa by Western analysis in two different human cell lines. Overexpression of hARSA-I in the embryonal human kidney 293 cell line was accompanied by overproduction of the 37-kDa protein Biochemical analysis using the GST/hARSA-I fusion protein indicated that hARSA-I is an ATPase analogous to the bacterial ArsA. Thus, hARSA-I is a new eukaryotic member of a highly conserved ATP-binding superfamily of proteins.
authors.affiliation: Department of Medicine, University of California San Diego, La Jolla 92093-0812, USA.
authors.forename: B
authors.initials: B
authors.lastname: Kurdi-Haidar
authors.forename: S
authors.initials: S
authors.lastname: Aebi
authors.forename: D
authors.initials: D
authors.lastname: Heath
authors.forename: R E
authors.initials: RE
authors.lastname: Enns
authors.forename: P
authors.initials: P
authors.lastname: Naredi
authors.forename: D K
authors.initials: DK
authors.lastname: Hom
authors.forename: S B
authors.initials: SB
authors.lastname: Howell
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D053501:Arsenite Transporting ATPases; D002460:Cell Line; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007106:Immune Sera; D016623:Ion Pumps; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009693:Nucleic Acid Hybridization; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D007106:Immune Sera; D016623:Ion Pumps; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases