Inositol phosphate turnover in human airways: effect of arachidonic acid metabolism.
The aim of this study was to examine the role of the phospholipase A2-arachidonic acid (PLA2-AA) pathway in response to histamine and the possible effect of AA metabolites on the generation of inositol-phosphates (IPs) in airway smooth muscle cells (SMC). Radiolabelled IPs-were analyzed by high-performance liquid chromatography (HPLC). Indomethacin, a cyclooxygenase inhibitor, potentiated the IP production evoked by a 5-sec application of histamine, while it decreased the effect of a 5-min incubation with the agonist, suggesting a time-dependent modulation of phospholipase C (PLC) activity by prostaglandins (PGs). Exogenous AA elicited the generation of IPs; this effect was suppressed by cyclooxygenase inhibition. Therefore, several steps in the AA metabolic pathways appear to modulate the production of IPs in human airway SMC.
Marmy
N
N
Institut de Physiologie, Fribourg, Switzerland.
Durand
J
J
D016718:Arachidonic Acid / Q000378:metabolism*; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*
D007295:Inositol Phosphates; D016718:Arachidonic Acid; D009637:Masoprocol; D006632:Histamine; D007213:Indomethacin