Effects of calcium channel blockers on steroidogenesis stimulated by ACTH and cAMP in isolated rat adrenal cells.
The effects of the calcium channel blockers verapamil, diltiazem, prenylamine and nifedipine on the ACTH- and cAMP-mediated pathway for steroidogenesis in a suspension solution of isolated rat adrenal cells were studied. Steroidogenesis stimulated by ACTH (10(-10) M) or cAMP (10(-3) M) was not inhibited by nifedipine (10(-4) M), but was strongly inhibited in a concentration-dependent manner by verapamil, diltiazem and prenylamine (10(-5) to 10(-4) M).
Matsuki
M
M
Department of Medicine, Kawasaki Medical School, Okayama, Japan.
Watanabe
S
S
Ikeda
M
M
Nishida
S
S
Saito
T
T
D000311:Adrenal Glands / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D004110:Diltiazem / Q000494:pharmacology; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D000324:Adrenocorticotropic Hormone; D014700:Verapamil; D000242:Cyclic AMP; D004110:Diltiazem; D009543:Nifedipine; D003345:Corticosterone