The effect of alpha-linolenic acid-rich emulsion on fatty acid metabolism and leukotriene generation of the colon in a rat model with inflammatory bowel disease.
The purpose of this study was to investigate the efficacy of alpha-linolenic acid-rich perilla oil emulsion (POE) in a rat model with trinitrobenzenesulfonic acid (TNB)-induced inflammatory bowel disease. Three different isocaloric solutions, which are glucose solution (FF), soybean oil emulsion (SOE) and POE, were infused for 14 days after instillation of TNB. After infusion, total cholesterol and phospholipid concentration in the plasma in the POE group were significantly decreased compared with the FF and SOE groups. Arachidonic acid level in the colonic phospholipids was significantly decreased and eicosapentaenoic acid level was significantly increased in the POE group compared with the FF and SOE groups. Thickness, damage score and leukotriene B4 content in the colon in the POE group were the lowest among the infusion groups. These results suggest that alpha-linolenic acid suppresses the synthesis of leukotriene B4 in the colon by changing the fatty acid composition in the colonic phospholipids and that POE may be effective in the improvement of inflammation in the colon.
Inui
K
K
Research Laboratories, Roussel Morishita Co., Ltd, Shiga, Japan.
Fukuta
Y
Y
Ikeda
A
A
Kameda
H
H
Kokuba
Y
Y
Sato
M
M
D000818:Animals; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D003106:Colon / Q000378:metabolism*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D007975:Leukotriene B4 / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects; D017962:alpha-Linolenic Acid / Q000494:pharmacology*
D005227:Fatty Acids; D010743:Phospholipids; D030262:Soybean Proteins; D017962:alpha-Linolenic Acid; D007975:Leukotriene B4; D014302:Trinitrobenzenesulfonic Acid; D002784:Cholesterol; D005947:Glucose