Occurrence of sex steroid hormones and their binding proteins in Octopus vulgaris lam.
The present study reports the presence of progesterone, testosterone and 17 beta-estradiol and their corresponding binding proteins in the reproductive system of Octopus vulgaris Lam (phylum Mollusca, subphylum Cephalopoda). These sex hormones occur in testis, vas deferens, seminal vesicle, prostate and Needham's sac. The hemolymph also contains a small, but significant, amount of these hormones and their carrier proteins. Among various tissues of the reproductive system, the seminal vesicle possesses the highest concentration of progesterone (4.8 ng/g tissue). The testis is the organ which contains the highest amount of testosterone (5.2 ng/g) whereas the prostate is the organ which contains the highest amount of 17 beta-estradiol (0.92 ng/g). The presence of these hormones has been ascertained by a radioimmunoassay method, an immunoenzymatic method and by a chemical (HPLC) method. Seatchard studies indicated that vas deferens and seminal vesicle contain specific sex steroid binding molecules at affinity levels comparable to those of vertebrate steroid receptors (0.5-5.0 pmol/g protein). In addition to the presence of the hormones, the delta 5,3 beta hydroxysteroid dehydrogenase, the key enzyme of steroidogenesis, also is found in testis. From a phylogenetic point of view, these findings are very interesting because they indicate a common origin of a sex hormonal system between Mollusca Cephalopoda and Vertebrates.
D'Aniello
A
A
Department of Biochemistry and Molecular Biology, Zoological Station of Naples, Italy.
Di Cosmo
A
A
Di Cristo
C
C
Assisi
L
L
Botte
V
V
Di Fiore
M M
MM
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D005837:Genitalia, Male / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D008297:Male; D049831:Octopodiformes / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013737:Testis / Q000201:enzymology
D012739:Gonadal Steroid Hormones; D011956:Receptors, Cell Surface; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase