Inhibin alpha and beta A subunit messenger ribonucleic acids expression in human pituitary adenomas: studies by quantitative reverse transcription-polymerase chain reaction.
Inhibin alpha and beta A subunit messenger ribonucleic acid (mRNA) levels were measured quantitatively by reverse transcription-polymerase chain reaction (RT-PCR) in human pituitary adenomas. The inhibin alpha subunit mRNA levels were undetectably low in cultured adenoma tissues, but beta A mRNA were 0.383 +/- 0.074 in 3 GH adenomas, 0.672 +/- 0.140 in 3 prolactinomas and 0.957 +/- 0.414 molecules/cell in 3 non-functioning adenomas. The addition of 10(-8) M activin A decreased the beta A mRNA levels within 4 h in 1 of 3 GH adenomas, 2 of 3 prolactinomas and 2 of 3 non-functioning adenomas, though the decreases were not statistically significant. The results showed an abundance of beta A subunit mRNA compared with alpha subunit mRNA in all human pituitary adenomas and a local role for activin in its own production through inhibin beta A mRNA subunit expression.
Suzuki
T
T
Department of Neurosurgery, Tokyo Women's Medical College, Japan.
Demura
R
R
Tajima
S
S
Yajima
R
R
Kubo
O
O
Takakura
K
K
Demura
H
H
D000236:Adenoma / Q000235:genetics*; D006801:Humans; D007265:Inhibins / Q000235:genetics*; D010911:Pituitary Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015175:Prolactinoma / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D013048:Specimen Handling
D012333:RNA, Messenger; D007265:Inhibins