title: Effects of a strain of Saccharomyces cerevisiae (Levucell SC1), a microbial additive for ruminants, on lactate metabolism in vitro.
abstract: The effect of Levucell SC, a strain of Saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria Streptococcus bovis and Megasphaera elsdenii. The coculture between 10(7) live cells x mL(-1) of SC and a Streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. Live yeast cells were able to compete with Streptococcus bovis for glucose utilization in strictly anaerobic conditions, so less glucose was available for the bacterium. SC also stimulated L-lactate utilization by a strain of M. elsdenii. The effect depended on the concentration of yeast cells added. Bacterial growth and fermentation end-product concentrations were also increased in the presence of SC. Some amino acids and vitamins, but not dicarboxylic acids, stimulated the bacterial specific activity of L-lactate uptake. SC was able to provide amino acids to M. elsdenii. In a coculture of Streptococcus bovis and M. elsdenii on glucose, the reduction of lactate concentration was improved by SC, the same trend being observed when maltose or soluble starch were used as carbon and energy source. These results indicate that SC can be a very useful tool to reduce lactate accumulation in vitro during fermentation of soluble sugars.
authors.affiliation: Laboratoire de microbiologie, Institut national de la recherche agronomique, Saint-Genès-Champanelle, France, fchauc@clermont.inra.fr
authors.forename: F
authors.initials: F
authors.lastname: Chaucheyras
authors.forename: G
authors.initials: G
authors.lastname: Fonty
authors.forename: G
authors.initials: G
authors.lastname: Bertin
authors.forename: J M
authors.initials: JM
authors.lastname: Salmon
authors.forename: P
authors.initials: P
authors.lastname: Gouet
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000693:Anaerobiosis; D000821:Animal Feed; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002264:Carboxylic Acids / Q000494:pharmacology; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D016985:Streptococcus bovis / Q000254:growth & development / Q000378:metabolism*; D045854:Veillonellaceae / Q000254:growth & development / Q000378:metabolism*; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000085:Acetates; D000596:Amino Acids; D002264:Carboxylic Acids; D014815:Vitamins; D019344:Lactic Acid; D005947:Glucose