title: Disturbance of cellular calcium homeostasis by in vitro application of shock waves.
abstract: Extracorporeally generated shock waves used in lithotripsy of urinary and biliary stones exhibit tissue lesions in vivo and destroy or damage cells in vitro. The involvement of cavitation-generated free radicals in these harmful effects is discussed controversially. We investigated changes in cytoplasmic calcium concentration and intracellular calcium localization after shock-wave treatment of suspended cell cultures using flow cytometry and electron microscopy and present evidence for the disturbance of mitochondrial Ca2+ a sequestration and, therefore, for a chemically induced cell injury.
authors.affiliation: Abteilung Biophysik, Universität Stuttgart, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Suhr
authors.forename: F
authors.initials: F
authors.lastname: Brümmer
authors.forename: U
authors.initials: U
authors.lastname: Irmer
authors.forename: D F
authors.initials: DF
authors.lastname: Hülser
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D005434:Flow Cytometry; D019220:High-Energy Shock Waves; D006706:Homeostasis; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism* / Q000528:radiation effects; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: D002118:Calcium