title: Determination of benzo[a]pyrene in lipid-soluble liquid smoke (LSLS) by HPLC-FL.
abstract: We developed a method for the determination of low levels of the carcinogen benzo[a]pyrene (BaP) in lipid-soluble liquid smoke (LSLS). The method consists of partitioning the LSLS (2 g) between n-hexane and DMSO, purification of the DMSO extracts on Sep-Pak C18 Plus and then Silica Plus cartridges, and determination of the BaP in the isolated extract by HPLC with fluorescence detection. Detection and quantification limits were 0.049 microgram/l (0.024 microgram/kg of LSLS) and 0.089 microgram/l (0.045 mg/kg), respectively. Recovery (87%) and CV% (< or = 1.5) were satisfactory.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, Spain.
authors.forename: M S
authors.initials: MS
authors.lastname: García Falcón
authors.forename: S
authors.initials: S
authors.lastname: González Amigo
authors.forename: M A
authors.initials: MA
authors.lastname: Lage Yusty
authors.forename: M J
authors.initials: MJ
authors.lastname: López de Alda Villaizán
authors.forename: J
authors.initials: J
authors.lastname: Simal Lozano
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005421:Flavoring Agents / Q000032:analysis*; D012906:Smoke
chemical_list: D002273:Carcinogens; D005421:Flavoring Agents; D012906:Smoke; D001564:Benzo(a)pyrene