title: Fluorescent labelling of pyruvate carboxylase with 4-acetamido-4'-(iodacetyl)-aminostilbene-2,2'-disulfonic acid (AIDA) provides a means of monitoring substrate binding.
authors.affiliation: Department of Biochemistry, University of Western Australia, Nedlands, Australia.
authors.forename: P V
authors.initials: PV
authors.lastname: Attwood
authors.forename: M
authors.initials: M
authors.lastname: Powell
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D011766:Pyruvate Carboxylase / Q000737:chemistry* / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013267:Stilbenes; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents
chemical_list: C090885:4-acetamido-4'-((iodoacetyl)amino)stilbene-2,2'-disulfonate; D005456:Fluorescent Dyes; D013267:Stilbenes; D013439:Sulfhydryl Reagents; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D011766:Pyruvate Carboxylase