Immunodetection of beet necrotic yellow vein virus RNA3-encoded protein in different host plants and tissues.
The protein p25 open reading frame (ORF) of beet necrotic yellow vein virus-BNYVV RNA3 was cloned into bacterial expression vector downstream of the 5-'terminus part of beta-galactosidase ORF and the expressed p25 fusion protein was used to produce an antiserum. The latter was employed to detect the subcellular location of p25 in mechanically inoculated Tetragonia expansa, Chenopodium quinoa and sugarbeet leaves by Western blot assay. The results showed that p25 was present as a soluble protein only in the S30 fraction of T. expansa, C. quinoa and sugarbeet leaves infected with BNYVV.
Li
Y
Y
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R. China.
Wei
C
C
Tien
P
P
Pan
N
N
Chen
Z
Z
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000276:immunology / Q000302:isolation & purification*; D010944:Plants / Q000821:virology; D012328:RNA Viruses / Q000276:immunology / Q000302:isolation & purification*; D012367:RNA, Viral; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D000956:Antigens, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D001616:beta-Galactosidase