Standardized AgNOR analysis: its usefulness in surgical oncology.
Recent improvements both in the staining quality and computer-aided quantitation of silver-stained nucleolar organizer region (AgNOR)-associated proteins offer the possibility to reliably investigate these proteins on routinely processed archival material. This article deals with the historical background, the recent introduction of a standardized quantitation, the clinical relevance, and future perspective for AgNOR evaluation. It is specifically emphasized that AgNOR analysis after both standardized staining and computer-aided quantitation (as recommended by the Committee on AgNOR Quantitation of the European Society of Pathology) is now regarded as the gold standard whenever routinely formalin-fixed and paraffin-embedded material is investigated.
Ofner
D
D
Department of Surgery 1, University of Innsbruck, Austria.
Schmid
K W
KW
D002455:Cell Division; D006801:Humans; D009369:Neoplasms / Q000737:chemistry / Q000601:surgery / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000737:chemistry / Q000648:ultrastructure*; D011379:Prognosis; D016622:Silver Staining / Q000379:methods / Q000592:standards
D009687:Nuclear Proteins