title: Distribution of acidic fibroblast growth factor-like immunoreactivity in rat skeletal muscle fibers.
abstract: Acidic fibroblast growth factor (aFGF) is a mitogenic, angiogenic and neurotrophic growth factor which promotes proliferation, but delays differentiation of cultured myoblasts. Its mRNA is expressed in the skeletal muscle, however, the distribution of aFGF in the postnatal skeletal muscle is poorly characterized. In the present study, the distribution of aFGF-like immunoreactivity (LI) was examined in developing and adult rat skeletal muscle fibers. In addition, the effect of the transection of the sciatic nerve on aFGF-LI in calf muscle fibers was examined. From the first postnatal day on, aFGF-immunoreactive (IR) muscle fibers were observed in different calf muscles. From the 7th postnatal day on a large number of muscle fibers exhibited aFGF-LI in the soleus muscle, some in plantaris and only few in gastrocnemius and extraocular muscles. Double-labelling with fast-myosin antibody showed that aFGF-LI was restricted to the slow oxidative muscle fibers. aFGF-IR intrafusal muscle fibers were seen in developing and mature muscle spindles. In addition, aFGF-IR nerve fibers and myoneural junctions were observed in different muscles. Transection of the sciatic nerve did not noticeably alter the expression pattern of aFGF-LI in calf muscles during two-week period. The present study demonstrates aFGF-LI in the rat slow oxidative muscle fibers where it may have fiber-type specific functions in addition to its known trophic effects.
authors.affiliation: Department of Anatomy, University of Tampere, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Junttila
authors.forename: L
authors.initials: L
authors.lastname: Rechardt
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
authors.forename: M
authors.initials: M
authors.lastname: Pelto-Huikko
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016220:Fibroblast Growth Factor 1