Suppression of enterotoxin-induced intestinal fluid secretion by wood creosote.
Wood creosote suppresses intestinal fluid secretion induced by heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC). When rabbit jejunum is ligated into a 5-cm segment and LT is administered locally, it actively induces intestinal fluid secretion in a dose-dependent manner. Local administration of wood creosote together with a fixed dose of LT suppressed the LT-induced fluid secretion in a dose-dependent manner. At a 50-ng/segment dose of LT, 7.4 +/- 1.1 ml (n = 5) of fluid is secreted into an intestinal segment; coadministration of wood creosote (150 micrograms/segment) significantly (p < 0.01) suppressed the fluid secretion to 2.4 +/- 2.3 ml. Based on these results, we conclude that the antidiarrheal activity of wood creosote is attributable to its antisecretory or proabsorptive effect (or both) on the intestine.
Ataka
K
K
Research Institute, Taiko Pharmaceutical Co., Ltd., Osaka, Japan.
Ogata
N
N
Kuge
T
T
Shibata
T
T
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D001834:Body Water / Q000378:metabolism; D010856:Columbidae; D003407:Creosote / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000633:toxicity*; D029968:Escherichia coli Proteins; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D003407:Creosote; C027538:heat-labile enterotoxin, E coli