Gene transfer mediated by alpha2-macroglobulin.
alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in vitro. The alpha2-macroglobulin receptor is a very large and multifunctional cell surface receptor, whose rapid and efficient internalization rate makes it attractive for gene therapy, e.g. for hepatic gene targeting via injection into the portal vein.
Schneider
H
H
Department of Internal Medicine III, University of Leipzig, Germany.
Huse
K
K
Birkenmeier
G
G
Otto
A
A
Scholz
G H
GH
D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008156:Luciferases / Q000235:genetics*; D011107:Polylysine / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins / Q000378:metabolism*
D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins; D011107:Polylysine; D008156:Luciferases