Characterization by Western blotting of mouse intestinal glycoproteins bound by Escherichia coli heat-labile enterotoxin type I.
Escherichia coli heat-labile enterotoxin type I (LT-I)-binding galactoproteins, which were not recognized by cholera toxin, were detected in intestinal epithelial cells of BALB/c mouse by Western blotting. Inhibitory studies using lectins and modifications of sugar chain suggest that LT-I recognizes certain mucin-type sugar chains containing the terminal Galbeta1-3GalNAc sugar sequence in the galactoproteins. The terminal sugar sequence is identical to that of GM1 ganglioside, the well-documented functional receptor for LT-I.
Shida
K
K
Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan.
Takamizawa
K
K
Takeda
T
T
Osawa
T
T
D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology; D015153:Blotting, Western; D004768:Enterotoxins / Q000302:isolation & purification* / Q000502:physiology; D004926:Escherichia coli / Q000737:chemistry*; D005677:G(M1) Ganglioside / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification* / Q000502:physiology; D007422:Intestines / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding
D001426:Bacterial Proteins; D004768:Enterotoxins; D006023:Glycoproteins; D005677:G(M1) Ganglioside