title: ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor.
abstract: To identify the functional P2 purinoceptor subtype in glomerular mesangial cells (GMC), polymerase chain reaction coupled with reverse transcription (RT-PCR) were performed, and cytosolic free calcium concentration ([Ca2+]i) was determined. RT-PCR analysis revealed that the molecular identity of P2 purinoceptor localized to GMC was both P2U and P2Y. The rank order of potency in stimulating [Ca2+]i was ATP [symbol: see text] UTP > 2-methylthio-ATP > ADP [symbol: see text] adenosine [symbol: see text] AMP. In addition, cross-desensitization between ATP and UTP occurred. In conclusion, ATP induces increase in [Ca2+]i via P2U purinoceptor in GMC.
authors.affiliation: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Kawamura
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Endou
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D005920:Glomerular Mesangium / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism*; D058473:Receptors, Purinergic P2Y2; D014158:Transcription, Genetic; D014544:Uridine Triphosphate / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; C546386:P2ry2 protein, mouse; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate