Lateral diffusion in planar lipid bilayers: a fluorescence recovery after photobleaching investigation of its modulation by lipid composition, cholesterol, or alamethicin content and divalent cations.
In spite of the fact that planar lipid bilayers are still the best-suited artificial membrane system for the study of reconstituted ion channels and receptors, data dealing with their physical characterization, especially as regards dynamics, are scanty. A combined electrical and optical chamber was designed and allowed fluorescence recovery after photobleaching recovery curves to be recorded from stable virtually solvent-free bilayers. D, the lateral diffusion coefficient of N-(7-nitrobenzoyl-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn- glycero-3-phosphoethanolamine, was found to be relatively insensitive to the phospholipid composition (headgroup, chain unsaturation, etc.), whereas inclusion of 33-50% cholesterol in the membrane reduced D by a factor of 2. Divalent cations significantly reduced D of negatively charged bilayers. These results compare well with data gathered on other model and natural systems. In addition, the incorporation of the voltage-dependent pore-former alamethicin did slightly reduce lipid lateral mobility. This study demonstrates the feasibility of such experiments with planar bilayers, which are amenable to physical constraints, and thus offers new opportunities for systematic studies of structure-function relationships in membrane-associating molecules.
Ladha
S
S
Institute of Food Research, Norwich, England. ladhas@bbsrc.ac.uk
Mackie
A R
AR
Harvey
L J
LJ
Clark
D C
DC
Lea
E J
EJ
Brullemans
M
M
Duclohier
H
H
D000408:Alamethicin / Q000737:chemistry; D055592:Biophysical Phenomena; D001703:Biophysics; D002118:Calcium; D002413:Cations, Divalent; D002784:Cholesterol / Q000737:chemistry; D004058:Diffusion; D004553:Electric Conductivity; D005453:Fluorescence; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007476:Ionophores / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D010714:Phosphatidylethanolamines; D010743:Phospholipids / Q000737:chemistry
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D007476:Ionophores; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D000408:Alamethicin; C042077:N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine; D002784:Cholesterol; D002118:Calcium