Resuscitation with increasing doses of diaspirin crosslinked hemoglobin in swine.
This study examined the effects of administering 0.5, 4, 10, and 30 mL/kg of Diaspirin Crosslinked Hemoglobin (DCLHb) in a swine model of non-lethal hemorrhagic shock. Thirty unanesthetized animals were bled (30 mL/kg, 1 mL/kg/min) and either recovered without treatment (Untreated Control, UC) or infused with 10 g/dL DCLHb (0.5, 4.0, 10 or 30 mL/kg at 1 mL/kg/min) or Lactated Ringer (LR, 90 mL/kg at 3 mL/kg/min). DCLHb caused dose-related increases in MAP. Both the 10 and 30 mL/kg doses of DCLHb increased MAP more than UC or LR. Lower doses of DCLHb and LR had effects on MAP similar to UC. After hemorrhage, CO increased in all groups. The effect of DCLHb on CO was dose-related. Only LR and 30 mL/kg of DCLHb transiently (through 90 min) increased CO more than UC. CO in animals given lower doses of DCLHb was comparable to UC. DCLHb (10 and 30 mL/kg) improved base excess and lactate concentrations, two indices of global perfusion, more rapidly and to a greater extent than either UC or LR. In this swine model of hemorrhage, even small doses of DCLHb exerted measurable beneficial effects on blood pressure and perfusion.
Marchand
G
G
Corporate Research and Technical Services, Baxter Healthcare Corporation Round Lake, Illinois 60073, USA.
Dunlap
E
E
Farrell
L
L
Nigro
C
C
Burhop
K
K
D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001774:Blood Chemical Analysis; D001802:Blood Substitutes / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006439:Hemodynamics; D006454:Hemoglobins / Q000627:therapeutic use*; D008297:Male; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000188:drug therapy*; D013552:Swine
D001802:Blood Substitutes; D006454:Hemoglobins; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin