The effect of mefloquine and quinine on the relaxation times of water protons in human erythrocytes, as revealed by NMR.
Effects of mefloquine and quinine on human erythrocyte membranes were investigated by 1H-NMR spectroscopy. The observed spin-spin relaxation time (T2) of intracellular water protons was found to reflect water permeability of the membrane. The effects of these drug treatments on the erythrocytes were determined by the ratio of the T2 of control and experimental samples. Comparing the results of quinine and mefloquine treatments, quinine decreased the T2 ratio to 0.79 at 5 mM and mefloquine increased the ratio to 1.14 at 0.5 mM. These results demonstrated that quinine increases water permeability through the membranes whereas mefloquine decreases it.
Nishina
M
M
Department of Medical Zoology, Saitama Medical School, Saitama, Japan.
Matsushita
K
K
Kato
K
K
D000328:Adult; D000962:Antimalarials / Q000494:pharmacology*; D001834:Body Water / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry* / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015767:Mefloquine / Q000494:pharmacology*; D011522:Protons; D011803:Quinine / Q000494:pharmacology*
D000962:Antimalarials; D011522:Protons; D011803:Quinine; D015767:Mefloquine