title: Comparison of low potassium Euro-Collins solution and standard Euro-Collins solution in an extracorporeal rat heart-lung model.
abstract: OBJECTIVE
  Euro-Collins solution (EC) is routinely used in lung transplantation. The high potassium of EC, however, may damage the vascular endothelium, thereby contributing to postischemic reperfusion injury. To assess the influence of the potassium concentration on lung preservation, we evaluated the effect of a "low potassium Euro-Collins solution" (LPEC), in which the sodium and potassium concentrations were reversed.
  
  
  METHODS
  In an extracorporeal rat heart-lung model lungs were preserved with EC and LPEC. The heart-lung blocks (HLB) were perfused with Krebs-Henseleit solution containing washed bovine red blood cells and ventilated with room air. The lungs were perfused via the working right ventricle with deoxygenated perfusate. Oxygenation and pulmonary vascular resistance (PVR) were monitored. After baseline measurements, hearts were arrested with St. Thomas' solution and the lungs were perfused with EC or LPEC, or were not perfused (controls). The HLBs were stored for 5 min or 2 h ischemic time at 4 degrees C. Reperfusion and ventilation was performed for 40 min. At the end of the trial the wet/dry ratio of the lungs was calculated and light microscopic assessment of the degree of edema was performed.
  
  
  RESULTS
  After 5 min of ischemia oxygenation was significantly better in both preserved groups compared to the controls. Pulmonary vascular resistance was elevated in all three groups after 30 min reperfusion at both ischemic times. After 2 h of ischemia PVR of the group preserved with LPEC was significantly lower than those of the EC and controls (LPEC-5 min: 184 +/- 65 dynes * sec * cm-5, EC-5 min: 275 +/- 119 dynes * sec * cm * cm-5, LPEC-2 h: 324 +/- 47 dynes * sec * m-5, EC-2 h: 507 +/- 83 dynes * sec * cm-5). Oxygenation after 2 h of ischemia and 30 min reperfusion was significantly better in the LPEC group compared to EC and controls (LPEC: 70 +/- 17 mmHg, EC: 44 +/- 3 mmHg). The wet/dry ratio was significantly lower in the two preserved groups compared to controls (LPEC-5 min: 5.7 +/- 0.7, EC-5 min: 5.8 +/- 1.2, controls-5 min: 7.5 +/- 1.8, LPEC-2 h: 6.7 +/- 0.4, EC: 6.9 +/- 0.4, controls-2 h: 7.3 +/- 0.4).
  
  
  CONCLUSIONS
  We thus conclude that LPEC results in better oxygenation and lower PVR in this lung preservation model. A low potassium concentration in lung preservation solutions may help in reducing the incidence of early graft dysfunction following lung transplantation.
authors.affiliation: Department of Thoracic Surgery, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukuse
authors.forename: J M
authors.initials: JM
authors.lastname: Albes
authors.forename: H
authors.initials: H
authors.lastname: Brandes
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Demertzis
authors.forename: H J
authors.initials: HJ
authors.lastname: Schäfers
mesh_terms: D000818:Animals; D002417:Cattle; D004195:Disease Models, Animal; D005112:Extracorporeal Circulation; D006085:Graft Survival; D016041:Heart-Lung Transplantation / Q000379:methods; D008297:Male; D019188:Organ Preservation Solutions / Q000737:chemistry*; D010101:Oxygen Consumption; D011188:Potassium; D011652:Pulmonary Circulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D014655:Vascular Resistance
chemical_list: D019188:Organ Preservation Solutions; D011188:Potassium