Antigenicity of human and horse myoglobins.
Rabbit antisera against human myoglobin and horse myoglobin cross-reacted with both myoglobins but only one of them recognized human hemoglobin. Two mouse monoclonal antibodies anti-human myoglobin were obtained, but only one of them (No. 49) cross-reacted with horse myoglobin. Antibody No. 49 and rabbit antibodies reacted also with apo-, FITC- and treated with hydrochloric acid or TPCK-trypsin horse myoglobin, but their binding to myoglobin pretreated with NaOH was reduced. Thirteen peptides overlapping sequence of human myoglobin were synthesized on polyethylene pins. Rabbit and mouse polyclonal antibodies reacted with some of these peptides but no reaction was noted with mouse monoclonal antibodies. Two monoclonal antibodies were applied for specific immunoassay of human myoglobin.
Kochanowska
I E
IE
Biochemical Laboratory, Polish Academy of Sciences, Czerska, Wroclaw, Poland.
Kuropatwa
M
M
Rapak
A
A
Szewczuk
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D003429:Cross Reactions; D018604:Epitope Mapping; D006736:Horses; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000276:immunology*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D009211:Myoglobin