Native DNA fragments photocrosslinked to psoralen binds to anti-B and anti-Z DNA antibodies.
Calf thymus DNA fragments of varying size (100-600 bp) were covalently linked to psoralen in the presence of 365 nm UV light. The crosslinked species were separated from the monoadducts by hydroxyapatite column chromatography. The DNA-psoralen crosslink showed binding with naturally occurring anti-DNA autoantibodies. This was substantiated by the recognition of affinity purified IgG by DNA-psoralen crosslinks of varying DNA size. The photo-crosslinks exhibited considerable recognition of both monoclonal and polyclonal anti-Z-DNA antibodies in inhibition ELISA and band shift assay.
Arjumand
S
S
Department of Biochemistry, Faculty of Medicine, Aligarh, India.
Arif
Z
Z
Ali
A
A
Ali
R
R
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D005363:Ficusin / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D013950:Thymus Gland / Q000502:physiology
D000906:Antibodies; D017319:Photosensitizing Agents; D004247:DNA; D005363:Ficusin