Study on the production of human interferon alpha-2b expressed in Escherichia coli.
The production process of human interferon alpha-2b was established in our institute. The human interferon alpha-2b was expressed in E. coli by using PL promotor. The production process consists of high cell density fermentation, refolding of the inclusion bodies, chromatography with Sephadex G-50 and DEAE, without using monocloning antibody affinity chromatography. After purification, about 500 mg of the human interferon alpha-2b was obtained from 1 kg wet cell harvested from 10 L fermentation broth. The biological activity shows 1-2 x 10(8) IU/mg protein. This production process is very simple and easy to scale-up. If this process adapted, it will save a lot of facility investment, space, and labor. Therefore, the cost will be reduced.
Yan
Y
Y
Harbin Pharmaceutical Institute of China, Harbin, China.
Wang
X
X
Wu
A
A
Sun
Y
Y
D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D006801:Humans; D007218:Industrial Microbiology / Q000191:economics / Q000379:methods; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins