title: Rapid polymerase chain reaction method for detection of Kanagawa positive Vibrio parahaemolyticus in seafoods.
abstract: Detection of Kanagawa positive strains of Vibrio parahaemolyticus by polymerase chain reaction (PCR) was tested. Primer pairs for specific amplification of tdh gene fragment is described. The assay could detect contamination of seafood homogenate when PCR was performed using lysate prepared directly from fish homogenates. The sensitivity of the assay could be improved to detect less than 10 cells of V. parahaemolyticus by performing PCR after 8 h enrichment in alkaline peptone water.
authors.affiliation: Department of Fishery Microbiology, College of Fisheries, Mangalore, India.
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
authors.forename: G
authors.initials: G
authors.lastname: Sugumar
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
authors.forename: P J
authors.initials: PJ
authors.lastname: Reilly
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006460:Hemolysin Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D017747:Seafood / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D006460:Hemolysin Proteins