title: DNA chips: analysing sequence by hybridization to oligonucleotides on a large scale.
abstract: Complete sequences of genomes and comprehensive sets of cDNA sequences open the way to a hugh range of biological problems. There is a need for analytical methods that can deal with the large number of sequences in the data banks and, ideally, we would like to analyse all sequences together. Gel-based sequencing is a serial process that analyses one sequence at a time. Oligonucleotide arrays, or 'DNA chips', are miniature, parallel analytical devices, which could bring to sequence analysis and molecular genetics many of the advantages that semiconductor devices brought to computing.
authors.affiliation: Department of Biochemistry, University of Oxford, UK. ems@bioch.ox.ac.uk
authors.forename: E M
authors.initials: EM
authors.lastname: Southern
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D009841:Oligonucleotides; D004247:DNA