Determination of Phenmetrazine in urine by gas chromatography-mass spectrometry.
A sensitive and simple gas chromatographic--mass spectrometric method is described for the determination of the central nervous system (CNS) stimulant phenmetrazine in urine. The extraction and derivatization were combined into a single step with isooctane and methyl chloroformate. The limit of quantitation was 0.05 micrograms/mliters urine, and the method was linear up to 100 micrograms/mliters. The coefficients of variation (CV) for within-day runs were 1.2% and 2.4% (n = 5) for two controls containing 1.0 micrograms/mliters and 50 micrograms/mliters, respectively. During a six-month period, the same controls showed CVs of 9.1% and 8.7%, respectively (n = 40), indicating a somewhat lower between-run precision. Phenmetrazine was present in 83 out of 3000 urine samples that were screened for CNS stimulants during this period, and the concentrations ranged from 0.5-370 micrograms/mliters.
Kronstrand
R
R
Department of Forensic Chemistry, University Hospital, Linköping, Sweden.
Hatanpää
M
M
Jonsson
J A
JA
D000697:Central Nervous System Stimulants / Q000032:analysis* / Q000652:urine; D005561:Formates / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010633:Phenmetrazine / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*
D000697:Central Nervous System Stimulants; D005561:Formates; C014667:methyl chloroformate; D010633:Phenmetrazine