Isolation of genes encoding tRNA binding proteins by probing an expression library with unmodified tRNA.
A rapid method for isolating Escherichia coli genes encoding proteins which bind unmodified, but not modified, tRNA is described. The method is generally applicable, and can be used to clone many RNA binding proteins where a structured RNA ligand is known.
Gustafsson
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Department of Microbiology, University of Umeå, Sweden. claes@calvin.ucsf.edu
D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism*; D012366:RNA, Transfer, Val / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
D012366:RNA, Transfer, Val; D016601:RNA-Binding Proteins; D012343:RNA, Transfer