title: Characterization of matrix metalloproteinase (MMP-8 and -9) activities in the saliva and in gingival crevicular fluid of children with Down's syndrome.
abstract: Previous studies have shown increased susceptibility to periodontal diseases in children with Down's syndrome (DS). The mechanisms involved in the periodontal inflammatory processes in DS are not fully understood. The present study characterized the periodontal status of 9 non-institutionalized DS children 9 to 17 years old (mean 13.6 years) relative to their age-matched systemically and periodontally healthy controls. The periodontal status was assessed by visible plaque index (VPI), gingival bleeding index (GBI), and probing depth. We also assessed, by sodium dodecyl sulphate polyacrylamide gel electrophoresis/laser densitometry and by zymography, the collagenase and gelatinase activities in the gingival crevicular fluid (GCF) and saliva samples collected from DS patients and from the controls. Eight of the nine DS children showed a periodontium comparable to that seen in healthy controls; beginning alveolar bone loss was radiographically seen in the DS patient with deep periodontal pockets. The endogenously active collagenase and total collagenase activities were slightly higher in GCF of DS children compared to healthy controls. Western blot demonstrated that GCF collagenase of DS patients was human neutrophil collagenase (MMP-8 or collagenase-2), which occurred in 75 kDa proMMP-8 and in DS patients, but not in controls, also in 65 kDa active MMP-8 form and occasionally lower 40-50 kDa MMP-8 species. Zymographic analysis revealed the presence of 120 kDa (MMP-9 complexed with neutrophil gelatinase associated lipocalin or NGAL), 92 kDa (MMP-9) and 72 kDa (MMP-2) gelatinases in DS and control GCF. Especially in DS GCF MMP-9 occurred in part in 82-85 kDa activated form. Salivary collagenase in DS was high when compared to controls but of the same MMP-8 type as in control saliva. Our findings suggest that in vivo activated MMP-8 in GCF derived from triggered PMNs and/or cytokine-induced periodontal fibroblasts may reflect periodontal tissue and alveolar bone destruction seen in the early stages of gingivitis/periodontitis associated with Down's syndrome.
authors.affiliation: Department of Periodontology, University of Helsinki, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Halinen
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: T
authors.initials: T
authors.lastname: Ingman
authors.forename: T
authors.initials: T
authors.lastname: Salo
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
authors.forename: H
authors.initials: H
authors.lastname: Saari
mesh_terms: D000293:Adolescent; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000201:enzymology; D015153:Blotting, Western; D016022:Case-Control Studies; D002648:Child; D017364:Collagenases / Q000032:analysis*; D003720:Densitometry; D003774:Dental Plaque Index; D004198:Disease Susceptibility; D004314:Down Syndrome / Q000150:complications / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology / Q000209:etiology; D006801:Humans; D007834:Lasers; D008297:Male; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9; D009504:Neutrophils / Q000201:enzymology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000209:etiology; D010518:Periodontitis / Q000201:enzymology / Q000209:etiology; D011859:Radiography; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
chemical_list: D012471:Salivary Proteins and Peptides; D012967:Sodium Dodecyl Sulfate; D017364:Collagenases; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9