SC1: a marker for astrocytes in the adult rodent brain is upregulated during reactive astrocytosis.
Astrocytes are the most abundant cell type in the mammalian central nervous system (CNS), and are involved in many processes critical for normal CNS maintenance and function. We have used double-label immunocytochemistry and in situ analysis to show that the SPARC (secreted protein acidic and rich in cysteine)-related protein SC1, co-localizes with the astrocyte marker glial fibrillary acidic protein (GFAP) in the adult rodent brain. Thus, SC1 is an astrocyte marker that may be used to investigate astrocyte heterogeneity and analyze glial cell lineages during neural development. Consistent with the presence of SC1 and GFAP in astrocytes, both proteins were markedly upregulated following reactive astrocytosis induced by focal mechanical trauma. Therefore, SC1 may play an important role in reactive astrocytosis subsequent to a wide variety of neural trauma, including neurodegenerative diseases and acute neural damage.
McKinnon
P J
PJ
Roche Institute of Molecular Biology, Hoffman-LaRoche, Nutley, NJ 07110, USA.
Margolskee
R F
RF
D020410:Activated-Leukocyte Cell Adhesion Molecule; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D014951:Wounds, Stab / Q000378:metabolism / Q000473:pathology
D020410:Activated-Leukocyte Cell Adhesion Molecule; D016326:Extracellular Matrix Proteins; D005904:Glial Fibrillary Acidic Protein; D015335:Molecular Probes; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger