title: Fast purification and kinetic studies of the glycerol-3-phosphate dehydrogenase from the yeast Saccharomyces cerevisiae.
abstract: The glycerol-3-phosphate dehydrogenase has been purified from Saccharomyces cerevisiae 140-fold to electrophoretic homogeneity by a simple procedure involving affinity and ion exchange chromatography. The purified enzyme was most active at pH 6.8 and 51 degrees C. Its molecular mass was determined to be 45000 +/- 2000 Da by SDS-polyacrylamide gel electrophoresis. At physiological pH values the thermodynamic equilibrium constant was determined to be 3.5 x 10(-3) (M-1). Product inhibition as well as competitive inhibition patterns were found which clearly indicate that the kinetic mechanism of the glycerol-3-phosphate dehydrogenase is random bi-bi with the formation of dead-end complexes. In vivo concentrations of selected metabolites and kinetic expression for G3P-DH were used to explain regulatory properties of this enzyme under conditions of short-term glucose effect in Saccharomyces cerevisiae.
authors.affiliation: Institut für Bioverfahrenstechnik, Universität Stuttgart, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.forename: M
authors.initials: M
authors.lastname: Pietzsch
authors.forename: U
authors.initials: U
authors.lastname: Theobald
authors.forename: M
authors.initials: M
authors.lastname: Rizzi
mesh_terms: D001667:Binding, Competitive; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D009243:NAD; D005993:Glycerolphosphate Dehydrogenase; D005947:Glucose; D005990:Glycerol