Changes of expressions of phosphotyrosine phosphatases in rat hepatocellular carcinoma induced by 3'-methyl-4-dimethylamino-azobenzene.
Using rats with hepatocellular carcinoma induced by 3'-methyl-4-dimethylamino-azobenzene (3'-MeDAB), we evaluated the expression of a protein tyrosine phosphatase (PTPase) in the tumor region, non-tumorous region and control rat liver. The expression of SHPTP2 increased 4.1 fold (p < 0.05) in mRNA, 2.1 fold (p < 0.01) in cytosol fraction, and 5.1 fold (p < 0.05) in membrane fraction, respectively, at a protein level in the tumor region compared with control liver. The expression of other phosphatases, LAR, LRP, and PTPase1B, did not change significantly. SHPTP2 phosphatase activity in the tumor region from rats also increased compared with control, suggesting that an increase of this activity may parallel the expression of SHPTP2. This increase of expression of SHPTP2 may contribute to the progression of hepatocellular carcinoma in this rat model.
Saito
S
S
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Hashimoto
N
N
Tokita
K
K
Toyoda
M
M
Suzuki
Y
Y
Goldstein
B J
BJ
Suzuki
H
H
Makino
H
H
Saito
Y
Y
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D002273:Carcinogens; D008749:Methyldimethylaminoazobenzene; D017027:Protein Tyrosine Phosphatases