Detection and localization of the EaeA protein of attaching and effacing Escherichia coli O45 from pigs using a monoclonal antibody.
The eaeA-positive, attaching and effacing (A/E) O45 E. coli isolates from pigs express an EaeA protein with an estimated molecular weight of 97 kDa. In the present study, a monoclonal antibody was raised against the EaeA protein of an A/E O45 isolate. Cross reaction of the monoclonal antibody with the EaeA protein of A/E strain of the rabbit (RDEC-1), but not with those of A/E strains of the human (E2348/69) and dog (89-4221), was observed. Reactions of the monoclonal antibody to A/E isolates in the O45 serogroup on the ELISA varied among isolates and appeared to be correlated with in vivo A/E capacity of these isolates. The EaeA protein of A/E O45 E. coli has an apparent isoelectric point of 8.4 and is exposed on the bacterial surface. The monoclonal antibody provides a useful tool for characterization of the EaeA protein of E. coli isolates from pigs.
Zhu
C
C
Groupe de Recherche sur les Maladies Infectieuses du Porc, Université de Montréal, Faculté de Médecine Vétérinaire, Québec, Canada.
Ménard
S
S
Dubreuil
J D
JD
Fairbrother
J M
JM
D018829:Adhesins, Bacterial; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D002352:Carrier Proteins; D002451:Cell Compartmentation; D003429:Cross Reactions; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology; D029968:Escherichia coli Proteins; D013045:Species Specificity; D013552:Swine
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli