RT-PCR analysis of MMP-9 expression in human articular cartilage chondrocytes and synovial fluid cells.
This report presents a method for analyzing matrix metalloproteinase-9 (92 kD gelatinase) MMP-9 mRNA expression using reverse transcription polymerase chain reaction (RT-PCR). With this method, chondrocytes isolated from small samples of osteoarthritic cartilage showed significantly elevated signal for MMP-9 mRNA compared to normal cartilage. In addition, cells of synovial fluid samples aspirated from osteoarthritic joints also exhibited MMP-9 expression using this technique. RT-PCR proved to be a sensitive method for assessing MMP-9 regulation in osteoarthritic and normal cartilage, and may provide a useful index of arthritic cartilage and synovial fluid cell metabolism when limited tissue is available.
Tsuchiya
K
K
Orthopaedic Research Laboratory, Stanford University School of Medicine, California 94305-5341, USA.
Maloney
W J
WJ
Vu
T
T
Hoffman
A R
AR
Schurman
D J
DJ
Smith
R L
RL
D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9; D010003:Osteoarthritis / Q000201:enzymology / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013582:Synovial Fluid / Q000166:cytology / Q000201:enzymology*
D012333:RNA, Messenger; D017364:Collagenases; D020780:Matrix Metalloproteinase 9