Isolation of high-molecular-length DNA from human skin.
An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.
Bennett
P V
PV
Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Gange
R W
RW
Hacham
H
H
Hejmadi
V S
VS
Moran
M
M
Ray
S
S
Sutherland
B M
BM
D000328:Adult; D001706:Biopsy / Q000379:methods; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D019286:Endopeptidase K / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D012685:Sepharose; D012867:Skin / Q000737:chemistry*; D013048:Specimen Handling
D004247:DNA; D012685:Sepharose; D019286:Endopeptidase K