title: The mathematical model of subtractive hybridization and its practical application.
abstract: A novel theory of subtractive hybridization including (or based on) the kinetic model of this process was proposed. A computer program modeling the process of subtraction was developed. Basing on the theory, a novel method of subtractive hybridization was proposed allowing routine comparison of genomes and products of genome expression. The method was applied to studies of the genetic mechanisms of embryogenesis, regeneration, cell differentiation and tumor transformation.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia. ermolaevaola@glas.apc.org
authors.forename: O D
authors.initials: OD
authors.lastname: Ermolaeva
authors.forename: S A
authors.initials: SA
authors.lastname: Lukyanov
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D004626:Embryology; D007700:Kinetics; D008962:Models, Theoretical; D009693:Nucleic Acid Hybridization; D012038:Regeneration; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software