Monoclonal antibody affinities of structurally related modified nucleosides.
A scale of relative affinities of a series of 2'-deoxycytidine and cytidine (CD) derivatives was established based on the data of cross-reactivities of these compounds as well as the displacements obtained from a competitive ELISA. No correlation could be established between the nucleosides modifying structures and the affinities. This can be explained by the possibilities of the modifying structures of intra- and intermolecular nonimmunospecific interactions owing to their degree of functionalization.
Higginson-Clarke
D
D
Molecular Biology Department, National Center for Scientific Research, Havana, Cuba.
Guevara García
R
R
Rodríguez Tanty
C
C
López Brauet
A
A
López-Canovas
L
L
Riverón Rojas
A M
AM
Macías Cabrera
A
A
D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D003562:Cytidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D003841:Deoxycytidine; D003562:Cytidine; D004247:DNA