title: Neutrophil activation and chemotaxis after in vitro treatment with perfluorocarbon.
abstract: The in vitro effects of perfluorocarbon (PFC) on human neutrophil activation and chemotaxis were examined. Neutrophils were incubated, with and without PFC, and were analyzed for chemotaxis through 5-microns and 8-microns pore filters. Neutrophil activation was quantitated by flow cytometry. Activation studies showed that PFC-treated neutrophil samples (n = 6) produced only 39.83% +/- 25.9% (mean +/- SD) of matched control (n = 6) fluorescence. Chemotaxis studies showed that PFC-treated neutrophil samples (n = 8) had migration of only 18.63% +/- 6.5% (of control values) of neutrophils to the outer boundary of the 5-microns filters (n = 8 controls). The 8-microns pore filter migration (n = 8) was similarly low, with a mean outer boundary migration count of only 26% +/- 19.8% of the control (n = 8) value. Thus, neutrophils exposed to perfluorocarbon produce significantly less detectable H2O2 (P < .001) and have a significantly lower chemotactic response (P < .001).
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, NY, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Rossman
authors.forename: M G
authors.initials: MG
authors.lastname: Caty
authors.forename: G A
authors.initials: GA
authors.lastname: Rich
authors.forename: H L
authors.initials: HL
authors.lastname: Karamanoukian
authors.forename: R G
authors.initials: RG
authors.lastname: Azizkhan
mesh_terms: D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005434:Flow Cytometry; D005466:Fluorocarbons / Q000009:adverse effects*; D006801:Humans; D006842:Hydrocarbons, Brominated; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D012121:Respiration, Artificial; D012128:Respiratory Distress Syndrome / Q000188:drug therapy
chemical_list: D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron