Prolonged subordination stress increases Calbindin-D28k immunoreactivity in the rat hippocampal CA1 area.
Previously we observed that corticosteroids alter Calbindin-D28k immunoreactivity in the rat hippocampus. In the present study we investigated whether prolonged subordination stress, presumably producing elevated plasma corticosterone levels (1) altered the immunocytochemical distribution of the Ca(2+)-binding proteins Calbindin-D28k (CBir) and Parvalbumin (PVir) in the rat hippocampus, and (2) induced ongoing neurodegenerative changes using a silver impregnation method. Eight days of subordination stress reduced body weight, increased adrenal weight corrected for body weight and reduced thymus weight, indicating its effectiveness to produce a stressful situation. Stress increased CBir selectively in the CA1 pyramidal cell layer whereas PVir was not altered. Silver-impregnation revealed no ongoing neurodegenerative changes in any of the hippocampal subfields.
Krugers
H J
HJ
Department of Biological Psychiatry, University of Groningen, The Netherlands.
Koolhaas
J M
JM
Medema
R M
RM
Korf
J
J
D000818:Animals; D001835:Body Weight / Q000502:physiology; D064092:Calbindin 1; D064026:Calbindins; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G