title: Typing of Listeria monocytogenes by monocin and phage receptors.
abstract: One hundred strains of Listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. The monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. The results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and RAPD (random amplification of polymorphic DNA). The monocin/phage types were comparable in terms of discrimination with other methods for epidemiological investigations. The index of discrimination of using the monocin typing and phage receptor/reverse phage receptor method combination (0.99) for both the 87 epidemiologically unrelated strains and the epidemiologically important serogroup 4 strains was the highest of the seven different methods analysed. This combination of methods was simple, highly discriminatory and reproducible and can be carried out in a non-specialized laboratory. However, like most of the other Listeria typing methods, both the method and the indicator test strains need to be standardized.
authors.affiliation: Centre National de Référence des Listérias, WHO Collaborating Center for Foodborne Listeriosis, CHUV, Lausanne, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Bannerman
authors.forename: P
authors.initials: P
authors.lastname: Boerlin
authors.forename: J
authors.initials: J
authors.lastname: Bille
mesh_terms: D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000145:classification*; D001434:Bacteriophage Typing; D008089:Listeria monocytogenes / Q000145:classification*; D011991:Receptors, Virus / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D001430:Bacteriocins; D011991:Receptors, Virus