Modified India ink preparation for Cryptococcus neoformans in cerebrospinal fluid specimens.
A novel modified India ink technique for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid specimens is described. It employs 2% chromium mercury and India ink. This technique allows a clear identification of some external and internal structures of the organism. Three layers from the outer capsule that have previously been discerned only by electron microscopy are distinguished.
Zerpa
R
R
Universidad Nacional Mayor de San Marcos, Lima, Peru.
Huicho
L
L
Guillén
A
A
D002244:Carbon; D004396:Coloring Agents; D003453:Cryptococcosis / Q000134:cerebrospinal fluid*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D006801:Humans
D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon