Compartmentalization of calbindin and parvalbumin in different parts of rat rubrospinal neurons.
The distribution of calbindin-immunoreactive neurons in the red nucleus and the subcellular distribution of the calbindin and parvalbumin in tracer-identified rubrospinal neurons of the rat were studied. Only a fraction of the retrogradely labelled rubrospinal neurons was found to contain calbindin. These neurons filled the caudal part of the red nucleus and also appeared sporadically along the ventromedial border of the middle segment of the red nucleus. In addition to the somata, calbindin was found in the dendritic arbors of tracer-identified rubrospinal neurons, revealed by injecting the fluorescent dye Lucifer Yellow into their cell bodies. The axons of rubrospinal neurons located in the caudal red nucleus were marked by performing anterograde tracing with fluorescent dextran tracer in freshly prepared brainstem slices. Parvalbumin was found to locate in swellings along these tracer-identified axons as well as at their cut ends. The results indicate that calbindin and parvalbumin are segregated to the somadendritic and axonal compartments of the rat rubrospinal neurons, respectively. This anatomical segregation suggests that they may have different functions in neurons.
Wang
Y J
YJ
Department of Anatomy, College of Medicine, National Taiwan University, Taipei, R.O.C.
Liu
C L
CL
Tseng
G F
GF
D000818:Animals; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012012:Red Nucleus / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
D064026:Calbindins; D002135:Calcium-Binding Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G