Comparison of circulating phagocyte oxidative activity measured by chemiluminescence in whole blood and isolated polymorphonuclear leukocytes.
The generation of reactive oxygen species (ROS) was measured by chemiluminescence (CL) in whole blood and isolated polymorphonuclear leukocytes (PMNLs) in 113 subjects. There were no differences in ROS production in males and females, smokers and non-smokers and no age dependency. A significant correlation was found between whole blood CL parameters and between parameters derived from isolated cells, but there was no correlation when these parameters were compared using isolated PMNLs and whole blood. Variations in serum lipid levels did not account for the variability of CL parameters in blood or PMNLs. Both methodologies had the same validity in demonstrating priming of phagocytes. Zymosan-induced CL was more sensitive in detecting distinct functional states of phagocytes than FMLP-induced CL.
Kopprasch
S
S
Technical University of Dresden, Carl Gustav Carus Medical School, Department of Internal Medicine III, Dresden, Germany.
Graessler
J
J
Kohl
M
M
Bergmann
S
S
Schröder
H E
HE
D000328:Adult; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015203:Reproducibility of Results; D012737:Sex Factors; D013268:Stimulation, Chemical; D013312:Stress, Physiological / Q000097:blood; D015054:Zymosan / Q000494:pharmacology
D008055:Lipids; D017382:Reactive Oxygen Species; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan