Active immunization with complementary peptide PBM 9-1: preliminary evidence that it modulates experimental allergic encephalomyelitis in PL/J mice and Lewis rats.
The idiotype (Id) of T cells and possibly antibodies are involved in an Id network that may immunoregulate experimental allergic encephalomyelitis (EAE). Thus, the adoptive EAE in PL/J mice responding to myelin basic protein (MBP) peptide acetyl 1-9 can be modulated by monoclonal antibody (mAb) anti-Id generated by immunization with a peptide of inverted hydropathy to MBP peptide 1-9, designated as PBM 9-1. A cross-reactive Id between species can be recognized on the T cell receptor (TCR) of Vb8.2 restricted T cells in either PL/J mice or Lewis rats. The present study was undertaken to examine the vaccine effect of PBM 9-1 presented in the form of a multiple antigen peptide (MAP) to induce active immunity against active EAE in Lewis rats and active or adoptive EAE in PL/J mice. MAP-PBM 9-1 induced an antibody response in both Lewis rats and PL/J mice, but more in the former. A low level of anti-Id antibody, including a low level of reactivity with specific but not control T cells, was also detected in the sera collected before induction of or after recovery from EAE. Active immunization with MAP-PBM 9-1 had a protective effect on relapses of adoptive EAE in PL/J mice and could prevent active EAE in Lewis rats. A relationship was noted between the titer of serum anti-PBM 9-1 Ab and the protective effect of active immunization in Lewis rats. Although the mechanism of effect remains to be clarified, these results suggest that MAP-PBM 9-1 is a nonencephalitogenic candidate for protection against inflammatory demyelination.
Zhou
S R
SR
Department of Neurology, University of Alabama, Birmingham 35294-0007, USA.
Whitaker
J N
JN
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line / Q000276:immunology / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000517:prevention & control*; D005260:Female; D005434:Flow Cytometry; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology* / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology; D011485:Protein Binding / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D012008:Recurrence; D013601:T-Lymphocytes / Q000166:cytology; D014611:Vaccination
D004676:Myelin Basic Protein; D010446:Peptide Fragments