Effect of water diuresis and water restriction on expression of HSPs-27, -60 and -70 in rat kidney.
Expression of HSP-27, HSP-60 and HSP-70 was estimated in the cortex, outer medulla and inner medulla (papilla) of rats undergoing water diuresis or water restriction for two days. The mRNAs for HSP-27 and HSP-60 in renal papilla were two- to threefold greater in rats during water restriction than in those excreting a dilute urine, but levels of mRNA for HSP-70 were not reduced by water diuresis and Western analysis for HSP-70 protein showed no difference between water-loaded and water-restricted animals.
Medina
R
R
Department of Medicine, Beth Israel Hospital, Boston, Massachusetts, USA.
Cantley
L
L
Spokes
K
K
Epstein
F H
FH
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D004231:Diuresis / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007672:Kidney Cortex / Q000502:physiology; D007679:Kidney Medulla / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014868:Water Deprivation / Q000502:physiology*
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger