Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons.
Multilocus enzyme electrophoresis (MEE) analysis and comparisons of nearly complete 16S rRNA gene sequences (1416 nucleotide positions) were used to evaluate phylogenetic relationships among Serpulina hyodysenteriae strain B78T, S. innocens strain B256T, Brachyspira aalborgi strain 513AT, and eight uncharacterised strains of swine, avian, and human intestinal spirochaetes. From MEE analysis, nine strains could be assigned to five groups containing other intestinal spirochaetes (genetic distances between groups = 0.6-0.9). Chicken spirochaete strain C1 and B. aalborgi 513AT represented unique electrophoretic types and formed their own MEE groups. Despite MEE differences, the 11 strains had highly similar (96.3-99.9%) 16S rRNA sequences. These findings point out limitations of both MEE analysis and 16S rRNA sequence comparisons when used as solitary techniques for classifying intestinal spirochaetes related to Brachyspira/Serpulina species.
Stanton
T B
TB
National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010, USA. tstanton@iastate.edu
Trott
D J
DJ
Lee
J I
JI
McLaren
A J
AJ
Hampson
D J
DJ
Paster
B J
BJ
Jensen
N S
NS
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017401:Brachyspira hyodysenteriae / Q000201:enzymology / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods*; D004798:Enzymes / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013145:Spirochaetales Infections / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology
D004798:Enzymes; D012336:RNA, Ribosomal, 16S