A polymerase chain reaction assay for the detection of Wuchereria bancrofti in blood samples from French Polynesia.
A polymerase chain reaction (PCR) assay based on a highly repeated deoxyribonucleic acid (DNA) sequence found in Wuchereria bancrofti (the SspI repeat) has been developed to address the shortcomings of traditional diagnostic methods. In this field study in a W. bancrofti endemic region of French Polynesia, 373 human blood samples were collected and 100 microL of blood were screened by the SspI PCR assay and 1 microL by membrane filtration. The SspI PCR assay detected 99 of 113 blood samples in which microfilariae had been detected by filtration (sensitivity of 88%) with a specificity of 100%. All the samples missed by the SspI PCR assay had less than 8 microfilariae per mL of blood. To evaluate the efficacy of screening larger blood samples by PCR, both 100 microL and 500 microL samples from 50 patients with very low-level microfilaraemia were screened by the SspI PCR assay; the sensitivity increased from 60% to 84% when using the larger volume of blood. Finally, an enzyme-linked immunosorbent assay-based version of the SspI PCR assay was used to screen blood from 12 patients following treatment with diethylcarbamazine, ivermectin, or both. These results showed that the PCR assay closely paralleled the presence or absence of microfilariae in the blood and that no increase in the DNA level was seen immediately following drug treatment.
Williams
S A
SA
Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA.
Nicolas
L
L
Lizotte-Waniewski
M
M
Plichart
C
C
Luquiaud
P
P
Nguyen
L N
LN
Moulia-Pelat
J P
JP
D000328:Adult; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004049:Diethylcarbamazine / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis* / Q000188:drug therapy; D005369:Filaricides / Q000627:therapeutic use; D006801:Humans; D007559:Ivermectin / Q000627:therapeutic use; D008297:Male; D008969:Molecular Sequence Data; D018512:Parasitemia / Q000175:diagnosis* / Q000188:drug therapy; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014958:Wuchereria bancrofti / Q000302:isolation & purification*
D005369:Filaricides; D007559:Ivermectin; D004247:DNA; D004049:Diethylcarbamazine