Effect of cyclo-oxygenase inhibition on in vitro B-cell function after burn injury.
The role of PGE2 in suppression of B-cell function after burn injury was investigated. Splenocytes from burned or sham-burned mice were isolated 8 days after burn injury and cultured with lipopolysaccharide with or without the addition of prostaglandin E2 (PGE2) or indomethacin (Indo). Anti-peptidoglycan polysaccharide immunoglobulin (Ig)M (specific antibody to a bacterial antigen), total IgM, and total IgG levels in culture supernatant and lymphocyte proliferation were measured. All B-cell functions were significantly suppressed by burn injury. PGE2 suppressed all B-cell functions except for IgG synthesis. Indo restored anti-peptidoglycan polysaccharide IgM to normal levels, but did not have a significant effect on suppressed proliferation and total IgM synthesis. IgG synthesis was increased by PGE2 and inhibited by Indo. Although not all B-cell suppression was accounted for by PGE2, this prostaglandin appeared to be a mechanism responsible for impaired antigen specific antibody response and isotype switching. Successful restoration of specific antibody synthesis to bacterial antigen suggests a potential therapeutic role for a cyclo-oxygenase blocking agent after burn injury.
Yamamoto
H
H
Department of Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7210, USA.
Siltharm
S
S
deSerres
S
S
Hultman
C S
CS
Meyer
A A
AA
D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D002056:Burns / Q000276:immunology*; D002455:Cell Division; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000502:physiology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000276:immunology / Q000502:physiology; D007075:Immunoglobulin M / Q000276:immunology / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000942:Antigens, Bacterial; D016861:Cyclooxygenase Inhibitors; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015232:Dinoprostone; D007213:Indomethacin