title: Thin-layer chromatographic determination of catecholamines in rat plasma.
abstract: A TLC method for the quantitative determination of epinephrine, norepinephrine and dopamine in rat plasma is described. After deprotenization, catecholamines were adsorbed on acid-alumina and acetylated. The acetyl derivatives were extracted using a C18 minicolumn, resolved on HPTLC plates and quantitated by fluorescence densitometry at 415 nm, using isoprenaline as internal standard.
authors.affiliation: Dept. de Biología Fonamental i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Alemany
authors.forename: M
authors.initials: M
authors.lastname: Akaârir
authors.forename: C
authors.initials: C
authors.lastname: Rosselló
authors.forename: A
authors.initials: A
authors.lastname: Gamundi
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000097:blood*; D002855:Chromatography, Thin Layer / Q000379:methods*; D002940:Circadian Rhythm; D003720:Densitometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D002395:Catecholamines