title: The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism.
abstract: We have isolated the predominant cytoplasmic histone acetyltransferase activity from Saccharomyces cerevisiae. This enzyme acetylates the lysine at residue 12 of free histone H4 but does not modify histone H4 when packaged in chromatin. The activity contains two proteins, Hat1p and Hat2p. Hat1p is the catalytic subunit of the histone acetyltransferase and has an intrinsic substrate specificity that modifies lysine in the recognition sequence GXGKXG. The specificity of the enzyme in the yeast cytoplasm is restricted relative to recombinant Hat1p suggesting that it is negatively regulated in vivo. Hat2p, which is required for high affinity binding of the acetyltransferase to histone H4, is highly related to Rbap48, which is a subunit of the chromatin assembly factor, CAF-1, and copurifies with the human histone deacetylase HD1. We propose that the Hat2p/Rbap48 family serve as escorts of histone metabolism enzymes to facilitate their interaction with histone H4.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Parthun
authors.forename: J
authors.initials: J
authors.lastname: Widom
authors.forename: D E
authors.initials: DE
authors.lastname: Gottschling
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology*; D004261:DNA Replication; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000302:isolation & purification; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins; D012260:Ribonucleases; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis; D013379:Substrate Specificity; D014157:Transcription Factors / Q000737:chemistry
chemical_list: C489780:CNOT8 protein, human; D002843:Chromatin; D006657:Histones; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D012260:Ribonucleases; C079009:POP2 protein, S cerevisiae; D006655:Histone Deacetylases; D008239:Lysine