Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells.
Glycophosphatidylinositol (GPI)-linked Thy-1 molecules, well known cell surface markers of murine T cells, are present on the glomerular mesangial cells of the rat kidney. The administration of anti-Thy-1.1 MoAbs 1-22-3 and OX-7 to rats induces severe and mild complement-dependent mesangial proliferative glomerulonephritis, respectively. In order to determine whether protein-tyrosine kinase (PTK) activity is associated with Thy-1 molecules on rat mesangial cell surface, we performed an immune complex kinase assay, using anti-Thy-1 MoAbs 1-22-3 and OX-7, followed by reimmunoprecipitation with anti-phosphotyrosine, anti-fyn, anti-lck and anti-lyn antibodies. Physical association of PTK, p59fyn and p56/53lyn with Thy-1 molecules was demonstrated in cultured rat mesangial cells. The activities of these kinases detected in MoAb 1-22-3 precipitates were higher than those in MoAb OX-7 precipitates. These results suggest that Thy-1 molecule transduces some signals also in rat mesangial cells.
Narisawa-Saito
M
M
Department of Immunology, Niigata University School of Medicine, Japan.
Yamanashi
Y
Y
Morioka
T
T
Oite
T
T
Shimizu
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000276:immunology; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology* / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000276:immunology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000276:immunology* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D018800:Thy-1 Antigens; D011505:Protein-Tyrosine Kinases