A screening procedure for the determination of 13 oral anticoagulants and rodenticides.
A technique for the simultaneous identification and quantitation of 13 hydroxycoumarin and indandione anticoagulant drugs and rodenticides from human serum by reversed-phase liquid chromatography with diode-array detection has been developed. High-performance liquid chromatography was performed using gradient elution with an acetonitrile and phosphate buffer on a Nucleosil ODS column. Ultraviolet spectra from 200 to 400 nm were recorded on-line during the analysis and compared with spectra stored in a library. For the spiked 2 mL of serum, acidic and alkaline liquid-liquid double extraction with diethylether-ether acetate (50:50, v/v) was conducted, and recoveries greater than 60% for most compounds were found. The detection limit was approximately 25 or 50 ng/mL for all components except for difethialone and fluindione, for which it was approximately 100 ng/mL. The standard calibration curves were linear from the detection limit to 5000 ng/mL. The within-run precision coefficient of variation (CV) was less than 10%, and the between-run precision CV was less than 20%.
Lotfi
H
H
Laboratoire de Toxicologié, Faculté de Pharmacie, Limoges, France.
Dreyfuss
M F
MF
Marquet
P
P
Debord
J
J
Merle
L
L
Lachâtre
G
G
D000284:Administration, Oral; D000925:Anticoagulants / Q000097:blood*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007189:Indans / Q000097:blood; D015203:Reproducibility of Results; D012378:Rodenticides / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000925:Anticoagulants; D003374:Coumarins; D007189:Indans; D012378:Rodenticides