Expression of dystrophin Dp71 during PC12 cell differentiation.
The expression of dystrophin-protein 71 (Dp71) was investigated during nerve growth factor (NGF) induced differentiation of PC12 cells. A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was designed to measure Dp71 mRNA, whereas the Dp71 protein amount was evaluated by immunoblot analysis using an anti-dystrophin monoclonal antibody. Comparison with control cultures showed that Dp71 mRNA and protein levels increased in parallel with NGF treatment peaking with increments of 60% and 1.4 times, respectively. The upregulation of Dp71 expression during PC12 cells differentiation point at PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells.
Cisneros
B
B
Departmento de Genetica y de Biología Molecular, Centro de Investigación y de Estudios Avanzados, Avenida Instituto Politecnico Nacional 2508, México, D.F., Mexico.
Rendon
A
A
Genty
V
V
Aranda
G
G
Marquez
F
F
Mornet
D
D
Montañez
C
C
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013997:Time Factors
D016189:Dystrophin; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C085339:apo-dystrophin 1