title: Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine.
abstract: An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5c,10;P1.12,13;P1.7h,4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes.
authors.affiliation: Laboratory for the Control of Biological Products, National Institute for Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Claassen
authors.forename: J
authors.initials: J
authors.lastname: Meylis
authors.forename: P
authors.initials: P
authors.lastname: van der Ley
authors.forename: C
authors.initials: C
authors.lastname: Peeters
authors.forename: H
authors.initials: H
authors.lastname: Brons
authors.forename: J
authors.initials: J
authors.lastname: Robert
authors.forename: D
authors.initials: D
authors.lastname: Borsboom
authors.forename: A
authors.initials: A
authors.lastname: van der Ark
authors.forename: I
authors.initials: I
authors.lastname: van Straaten
authors.forename: P
authors.initials: P
authors.lastname: Roholl
authors.forename: B
authors.initials: B
authors.lastname: Kuipers
authors.forename: J
authors.initials: J
authors.lastname: Poolman
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D006801:Humans; D008585:Meningitis, Meningococcal / Q000517:prevention & control; D051379:Mice; D008854:Microscopy, Electron; D009345:Neisseria meningitidis / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines