Both the H13 gene product and 4F2 antigen are involved in the induction of system y+ cationic amino-acid transport following activation of human peripheral blood mononuclear cells (PBM).
Prior transfection with antisense oligonucleotides to the H13 and 4F2 hc genes, singly or in combination, was found to inhibit phytohaemagglutinin-induced activation of cationic amino-acid transport system y+ in human peripheral blood mononuclear cells (mostly circulating lymphocytes). These effects on system y+ function or expression mean that 4F2 hc cannot only be the molecular basis of system y+L (Fei, Y.-J., Prasad, P.D., Leibach, F.H. and Ganapathy, V. (1995) Biochemistry 34, 8744-8751).
Chen
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Department of Human Anatomy, University of Oxford, UK.
Meredith
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Boyd
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CA
D000596:Amino Acids / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D027261:Fusion Regulatory Protein-1; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000235:genetics*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011991:Receptors, Virus
D000596:Amino Acids; D015703:Antigens, CD; D002352:Carrier Proteins; D027261:Fusion Regulatory Protein-1; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011991:Receptors, Virus; C070506:ecotropic murine leukemia virus receptor