title: The rate of adaptive mutagenesis in Escherichia coli is enhanced by oxygen (superoxide).
abstract: Adaptive mutagenesis is that which occurs in non-dividing cells and which allows growth under the selective conditions imposed. We now report that reversion of amino acid auxotrophies in E. coli fits that definition and is enhanced under conditions conducive to oxidative damage to DNA. Thus adaptive mutagenesis was approximately 4-fold more frequent in a sodA sodB strain than in the superoxide dismutase-replete parental strain and this mutagenesis was suppressed under anaerobic conditions. Moreover, a cell permeant manganic porphyrin, capable of catalyzing the dismutation of O2-, diminished the rate of occurrence of these mutations. Repair of oxidative damage to DNA, in the non-dividing cells, appears to provide the opportunity for adaptive mutagenesis.
authors.affiliation: Department of Biochemistry Duke UniveRsity Medical Center, Durham, NC 27710, USA.
authors.forename: L
authors.initials: L
authors.lastname: Benov
authors.forename: I
authors.initials: I
authors.lastname: Fridovich
mesh_terms: D000581:Amidohydrolases / Q000235:genetics; D001120:Arginine / Q000378:metabolism; D004249:DNA Damage; D004926:Escherichia coli / Q000235:genetics*; D016296:Mutagenesis; D010100:Oxygen / Q000737:chemistry*; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000502:physiology; D013481:Superoxides / Q000737:chemistry
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D001120:Arginine; D013482:Superoxide Dismutase; D000581:Amidohydrolases; C018732:N-acetylornithine deacetylase; D010100:Oxygen