Cytochrome P-450 and free radical generation in rat liver microsomes under the influence of prostaglandin E1.
There is evidence to suggest that the mechanism of antioxidant effect of prostaglandin E1 (PGE1) is due to decrease of radical species generation by cytochrome P-450 in rat liver microsomes. Chronic alcohol intoxication increased NADPH oxidation, cytochrome P-450 content and NADPH-stimulated chemoluminiscence of microsomes. Ethanol also raised superoxide dismutase (SOD) activity in microsomes. PGE1 decreased cytochrome P-450 content, normalized NADPH oxidation, NADPH-induced chemoluminiscence and SOD activity in the liver of alcohol-treated rats. PGE developed the similar effect after microsomal induction by both acetone combined with starvation and phenobarbital normalizing all the above parameters. Therefore, PGE1 affects on both, ethanol-inducible IIE1 and phenobarbital-inducible IIB1 isoforms.
Buko
V U
VU
Institute of Biochemistry, Belarussian Academy of Sciences, Grodno, Belarus.
Sadovnichy
V V
VV
D000096:Acetone / Q000008:administration & dosage; D000527:Alprostadil / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D015536:Down-Regulation; D000431:Ethanol / Q000008:administration & dosage; D005609:Free Radicals / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D010634:Phenobarbital / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D000096:Acetone; D000431:Ethanol; D009249:NADP; D006861:Hydrogen Peroxide; D019392:Cytochrome P-450 CYP2E1; D019362:Cytochrome P-450 CYP2B1; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C069506:NADH-cytochrome P-450 reductase; D019255:NADPH Oxidases; D000527:Alprostadil; D010634:Phenobarbital