title: The relevance of chondroitin and keratan sulphate markers in normal and arthritic synovial fluid.
abstract: This study investigated the synovial fluid concentrations of glycosaminoglycan (GAG), keratan sulphate (KS) epitope 5D4 and chondroitin sulphate (CS) sulphation patterns in healthy volunteers and patients with osteoarthritis (OA) and rheumatoid arthritis (RA). Synovial fluids were collected from knee joints of healthy volunteers (n = 24), and patients with OA (n = 28) and RA (n = 29). Concentrations of GAG and the keratan sulphate epitope 5D4 were measured in 15 of the healthy volunteers, and all of the OA and RA synovial fluids. Total GAG was measured using a dye-binding method and 5D4 by an ELISA. The unsaturated CS disaccharides delta C4 and delta C6 were measured by capillary electrophoresis in all synovial fluids. The concentrations of GAG, 5D4 and delta C6 in the normal synovial fluid were higher but that of delta C4 lower than those of the disease groups. The delta C6:delta C4 ratios correlated with age (r = -0.437, P < 0.001) and the mean value was lower in females than males (2.92 compared with 5.22, P < 0.001). After allowing for age and sex, the delta C6:delta C4 ratio in the control group was significantly elevated (P < 0.001) compared to both OA and RA. The ratio was also related to proteoglycan markers (r = 0.383 for 5D4 and r = 0.357 for GAG). The finding that 5D4 and delta C6:delta C4 ratios are higher in synovial fluid from healthy volunteers compared to OA and RA suggests that they may be markers of the susceptibility of articular cartilage to early damage in arthritis.
authors.affiliation: University of Bristol Rheumatology Unit, Bristol Royal Infirmary.
authors.forename: M
authors.initials: M
authors.lastname: Sharif
authors.forename: D J
authors.initials: DJ
authors.lastname: Osborne
authors.forename: K
authors.initials: K
authors.lastname: Meadows
authors.forename: S M
authors.initials: SM
authors.lastname: Woodhouse
authors.forename: E M
authors.initials: EM
authors.lastname: Colvin
authors.forename: L
authors.initials: L
authors.lastname: Shepstone
authors.forename: P A
authors.initials: PA
authors.lastname: Dieppe
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000704:Analysis of Variance; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002356:Cartilage / Q000737:chemistry / Q000378:metabolism / Q000503:physiopathology; D002807:Chondroitin / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D018604:Epitope Mapping / Q000592:standards; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007632:Keratan Sulfate / Q000032:analysis*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D012737:Sex Factors; D013455:Sulfur / Q000378:metabolism; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D004187:Disaccharides; D006025:Glycosaminoglycans; D013455:Sulfur; D002807:Chondroitin; D007632:Keratan Sulfate