Demonstration of an osteoblast defect in two cases of human malignant osteopetrosis. Correction of the phenotype after bone marrow transplant.
Osteopetrosis is an inherited disorder characterized by bone sclerosis due to reduced bone resorption. Here we report that human osteopetrotic osteoblast-like (Ob) cells express a defective phenotype in primary cultures in vitro, and that bone marrow transplant (BMT) corrects osteoblast function. DNA analysis at polymorphic short-tandem repeat loci from donor, recipient, and primary Ob-like cells pre-BMT and 2 yr post-BMT revealed that Ob were still of recipient origin post-BMT. Osteopetrotic Ob-like cells obtained pre-BMT showed normal and abnormal 1,25(OH)2D3-induced alkaline phosphatase (ALPase) and osteocalcin production, respectively, and failed to produce macrophage colony-stimulating factor (M-CSF) in response to IL-1a and TNF-alpha. These parameters were all normalized in primary Ob-like cells prepared 2 yr post-BMT. X-linked clonality analysis at the human androgen receptor (HUMARA) locus revealed that osteoblasts showed a polyclonal and an oligoclonal derivation pre- and post-BMT respectively, indicating that a limited number of progenitor reconstituted this population. Because osteoblasts were still of recipient origin post-BMT, this suggests that functional osteoclasts, due to the replacement of hematopoeitic cells, provided a local microenvironment in vivo triggering the differentiation and/or recruitment of a limited number of functional osteoblasts.
Lajeunesse
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D
Centre de Recherche Guy Bernier et Unité de Greffe de Moëlle Osseuse,Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada.
Busque
L
L
Ménard
P
P
Brunette
M G
MG
Bonny
Y
Y
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D016026:Bone Marrow Transplantation; D002117:Calcitriol / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D007223:Infant; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D010022:Osteopetrosis / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics; D014960:X Chromosome
D011944:Receptors, Androgen; D015675:Osteocalcin; D016173:Macrophage Colony-Stimulating Factor; D000469:Alkaline Phosphatase; D002117:Calcitriol