title: Evaluation of an epoxy-fixed biological patch with ionically bound heparin as a pericardial substitute.
abstract: In an attempt to develop an improved pericardial substitute, we undertook the development of an epoxy-fixed biological patch with ionically bound heparin. The study was to evaluate the cross-linking characteristics of this newly developed biological patch using its glutaraldehyde-fixed counterpart as a control. In addition, the feasibility of using this newly developed biological patch as a pericardial substitute was assessed in a canine model. In the study, it was observed that the epoxy-fixed biological patch appeared more similar to the native pericardium in colour and was more pliable than its glutaraldehyde-fixed counterpart. Also, both the epoxy- and glutaraldehyde-fixed biological patches had significant increases in fixation index and denaturation temperature as compared to the fresh one (p < 0.05). In the canine study, the epoxy-fixed biological patch with ionically bound heparin was found to have significantly less adhesion formation than those currently used clinically (p < 0.05).
authors.affiliation: Department of Chemical Engineering, National Central University, Chung-Li, Taiwan.
authors.forename: H W
authors.initials: HW
authors.lastname: Sung
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
authors.forename: Y T
authors.initials: YT
authors.lastname: Chiu
authors.forename: H L
authors.initials: HL
authors.lastname: Hsu
authors.forename: C C
authors.initials: CC
authors.lastname: Shih
authors.forename: J H
authors.initials: JH
authors.lastname: Lu
authors.forename: P C
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004285:Dogs; D004852:Epoxy Compounds / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006493:Heparin / Q000378:metabolism*; D010496:Pericardium / Q000378:metabolism / Q000601:surgery*; D011489:Protein Denaturation; D013537:Sutures; D013552:Swine; D013696:Temperature; D016707:Tissue Fixation; D014183:Transplantation, Heterologous / Q000592:standards*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004852:Epoxy Compounds; D006493:Heparin; D005976:Glutaral