GM-CSF induces the tyrosine phosphorylation of three isoforms of Shc and its association with Grb2 in TF-1 cell.
This report demonstrates that GM-CSF induces the tyrosine phosphorylation of Shc protein which is implicated in Ras activation. Three isoforms of Shc are ubiquitously phosphorylated induced by GM-CSF in TF-1, a cell line of erythroid origin. It is also shown that Shc is associated with the adaptor protein Grb2. The formation of Shc-Grb2 complex may directly link tyrosine phosphorylation events to Ras activation in TF-1 cells.
Shi
X
X
Department of Biochemistry, Nanjing University, People's Republic of China.
Qin
J
J
Zhu
D
D
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051380:GRB2 Adaptor Protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D011233:Precipitin Tests; D011506:Proteins / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D011506:Proteins; C528024:SHC1 protein, human; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D014443:Tyrosine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D018631:ras Proteins