Imipenem resistance among Acinetobacter baumannii: association with reduced expression of a 33-36 kDa outer membrane protein.
The mechanism of imipenem resistance of two Acinetobacter baumannii isolates (A-1 and A-24) was characterized in this study. A spontaneous revertant (A-1 (rev)) derived from isolate A-1 showed susceptibility to imipenem. beta-Lactamase hydrolysis studies showed no evidence of an imipenem hydrolyzing enzyme among A-1, A-24, A-1 (rev), or two other imipenem susceptible A. baumannii isolates. Outer membrane protein (OMP) analysis indicated decreased expression of a 33-36 kDa protein by isolates A-1 and A-24 when compared with A-1 (rev) and the other A. baumannii isolates. In conclusion, decreased expression of a 33-36 kDa OMP is associated with imipenem resistance among A. baumannii.
Clark
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RB
Department of Microbiology, American Medical Laboratories, Inc., Chantilly, VA 22021, USA.
D000150:Acinetobacter / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D015378:Imipenem / Q000494:pharmacology*; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D013845:Thienamycins / Q000494:pharmacology*; D018440:beta-Lactam Resistance
D001425:Bacterial Outer Membrane Proteins; D013845:Thienamycins; D015378:Imipenem