Modelling cortical cataractogenesis. XVIII. In vitro diabetic cataract reduction by venoruton. A flavonoid which prevents lens opacification.
The effect of a novel flavonoid, venoruton (a mixture of mono-, di-, tri- and tetrahydroxyethylrutosides) has been investigated in healthy rat lenses and compared with diabetic cataract modelled in vitro. One mM venoruton was added to medium simulating healthy and diabetic conditions for the incubated lenses; damage was followed by either stereoscopic photography of the lenses under a Cooperative Cataract Research Group operating microscope or with our recently developed method: the leakage of lactate dehydrogenase (LDH) into the lens culture media. The increased LDH activity in the medium and observable development of the opacity were correlated with cell damage, which has been found to be associated with globular degeneration and cataract formation. The extent of opacification and LDH release is reduced if 1 mM venoruton is included in the medium. The protective effect may be related to antioxidant activity against reactive oxygen species: decreased luminol luminescence was shown after venoruton addition to either superoxide-generating hypoxanthine plus xanthine oxidase, or hydrogen peroxide.
Kilic
F
F
Department of Biochemistry, University of Western Ontario, London, Canada.
Bhardwaj
R
R
Trevithick
J R
JR
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002386:Cataract / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003470:Culture Media; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005260:Female; D005947:Glucose / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007904:Lens Cortex, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000494:pharmacology
D000975:Antioxidants; D003470:Culture Media; D006896:Hydroxyethylrutoside; D013481:Superoxides; C005865:troxerutin; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005947:Glucose