title: phi 20, a temperate bacteriophage isolated from Bacillus anthracis exists as a plasmidial prophage.
abstract: This study describes the isolation of temperate B. anthracis phages, from 4 out of 20 B. anthracis strains screened, by use of the inducing agents mitomycin C and UV light. Phage phi 20 isolated from B. anthracis Sterne 34F2 (pXO1+ pXO2-) was shown to have double-stranded DNA of size 48756 bp and a restriction site map showing nine sites for enzymes BamHI, BglII, and SstI is included. The phi 20 genome was found to exist as a plasmidial prophage and the phage itself to have a polyhedral head of diameter 65 nm and tail 217 nm long and 15 nm wide.
authors.affiliation: Public Health Laboratory Service, Centre for Applied Microbiology and Research, Salisbury, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Inal
authors.forename: K V
authors.initials: KV
authors.lastname: Karunakaran
mesh_terms: D017103:Bacillus Phages / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D001408:Bacillus anthracis / Q000821:virology*; D016679:Genome, Viral; D008854:Microscopy, Electron; D016685:Mitomycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification*; D015183:Restriction Mapping; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects / Q000528:radiation effects
chemical_list: D016685:Mitomycin