title: Expression of dystrophin Dp71 during PC12 cell differentiation.
abstract: The expression of dystrophin-protein 71 (Dp71) was investigated during nerve growth factor (NGF) induced differentiation of PC12 cells. A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was designed to measure Dp71 mRNA, whereas the Dp71 protein amount was evaluated by immunoblot analysis using an anti-dystrophin monoclonal antibody. Comparison with control cultures showed that Dp71 mRNA and protein levels increased in parallel with NGF treatment peaking with increments of 60% and 1.4 times, respectively. The upregulation of Dp71 expression during PC12 cells differentiation point at PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells.
authors.affiliation: Departmento de Genetica y de Biología Molecular, Centro de Investigación y de Estudios Avanzados, Avenida Instituto Politecnico Nacional 2508, México, D.F., Mexico.
authors.forename: B
authors.initials: B
authors.lastname: Cisneros
authors.forename: A
authors.initials: A
authors.lastname: Rendon
authors.forename: V
authors.initials: V
authors.lastname: Genty
authors.forename: G
authors.initials: G
authors.lastname: Aranda
authors.forename: F
authors.initials: F
authors.lastname: Marquez
authors.forename: D
authors.initials: D
authors.lastname: Mornet
authors.forename: C
authors.initials: C
authors.lastname: Montañez
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013997:Time Factors
chemical_list: D016189:Dystrophin; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C085339:apo-dystrophin 1