Crystallization and preliminary x-ray crystallographic studies of L-2-haloacid dehalogenase from Pseudomonas sp. YL.
The dimeric L-2-haloacid dehalogenase from Pseudomonas sp. YL, (subunit mass, 26179 Da), has been crystallized by vapor diffusion, supplemented by repetitive seeding, against a 50 mM potassium dihydrogenphosphate solution (pH 4.5) containing 15% (w/v) polyethylene glycol 8,000 and 1% (v/v) n-propanol. The crystals belong to the monoclinic space group C2 with unit cell dimensions of a = 92.21 angstrom, b = 62.78 angstrom, c = 50.84 angstrom, and beta = 122.4 degrees, and contain two dehalogenase dimers in the unit cell. They are of good quality and diffract up to 1.5 angstrom resolution.
Hisano
T
T
Institute for Chemical Research, Kyoto University, Japan.
Hata
Y
Y
Fujii
T
T
Liu
J Q
JQ
Kurihara
T
T
Esaki
N
N
Soda
K
K
D018360:Crystallography, X-Ray; D006867:Hydrolases / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology*
D006867:Hydrolases; C034507:2-haloacid dehalogenase