title: Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.
abstract: Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infected cell suspensions and culture supernatants were compared among seven assays sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log10 virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistently low titers.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Miami, Florida 33101, USA. wscott@mednet.med.miami.edu
authors.forename: W A
authors.initials: WA
authors.lastname: Scott
authors.forename: D
authors.initials: D
authors.lastname: Brambilla
authors.forename: E
authors.initials: E
authors.lastname: Siwak
authors.forename: C
authors.initials: C
authors.lastname: Beatty
authors.forename: J
authors.initials: J
authors.lastname: Bremer
authors.forename: R W
authors.initials: RW
authors.lastname: Coombs
authors.forename: H
authors.initials: H
authors.lastname: Farzadegan
authors.forename: S A
authors.initials: SA
authors.lastname: Fiscus
authors.forename: S M
authors.initials: SM
authors.lastname: Hammer
authors.forename: F B
authors.initials: FB
authors.lastname: Hollinger
authors.forename: N
authors.initials: N
authors.lastname: Khan
authors.forename: S
authors.initials: S
authors.lastname: Rasheed
authors.forename: P S
authors.initials: PS
authors.lastname: Reichelderfer
mesh_terms: D018929:Cell Culture Techniques; D015925:Cryopreservation; D015658:HIV Infections; D015497:HIV-1; D006801:Humans; D013997:Time Factors