Susceptibility of growth factors to degradation by Helicobacter pylori protease: effect of ebrotidine and sucralfate.
In this study, we investigated the susceptibility of the growth factors (EGF, bFGF, TGF beta and PDGF) to degradation by the protease elaborated by H. pylori and assessed the effect of antiulcer agents, ebrotidine and sucralfate, on this enzymatic action of the bacterium. The colonies of H. pylori were washed with saline, filtered to retain the bacteria, and the filtrate used as an enzyme source. The assays revealed that while EGF and beta FGF showed only marginal (5-7%) susceptibility to H. pylori protease, a 61.7% degradation occurred with PDGF and 62.3% with TGF beta. Introduction of ebrotidine or sucralfate to the assay system led to the reduction in the rate of growth factors degradation, which at 50 micrograms/ml ebrotidine reached the value of 85.1% for PDGF and 88.6% for TGF beta, while with sucralfate the optimal inhibition of PDGF (79.6%) and TGF beta (82.7%) degradation occurred at 200 micrograms/ml. The results demonstrate that PDGF and TGF beta are susceptible to H. pylori degradation and that antiulcer agents, ebrotidine and sucralfate are capable of counteracting this effect.
Slomiany
B L
BL
Research Center, University of Medicine and Dentistry of New Jersey Newark 07103-2400, USA.
Piotrowski
J
J
Slomiany
A
A
D001557:Benzenesulfonates / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D016480:Helicobacter pylori / Q000201:enzymology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D013392:Sucralfate / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D001557:Benzenesulfonates; D006635:Histamine H2 Antagonists; D010982:Platelet-Derived Growth Factor; D013844:Thiazoles; D016212:Transforming Growth Factor beta; D013392:Sucralfate; D010450:Endopeptidases; C075156:ebrotidine