The effect of N-acetylcysteine on the in vitro growth of normal rabbit middle ear fibroblasts.
In the present study the effect of N-acetylcysteine on the growth pattern and collagen synthesis of cultures of rabbit middle ear fibroblasts was determined. The growth pattern was evaluated by cell counting, measurements of the total content of cell protein and mitotic activity by incorporation of 3H-thymidine. Collagen synthesis was estimated by incorporation of 3H-proline. The results demonstrate a dose-dependent reduction in both normal cell proliferation and collagen production. Thus, N-acetylcysteine seems to possess properties desirable and useful in the treatment of secretory otitis media.
Ovesen
T
T
ENT Department, Aarhus University Hospital, Denmark.
Gaihede
M
M
Ledet
T
T
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D018929:Cell Culture Techniques; D003094:Collagen / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004432:Ear, Middle / Q000187:drug effects*; D005100:Expectorants / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects; D008938:Mitosis; D010034:Otitis Media with Effusion; D011817:Rabbits
D005100:Expectorants; D008565:Membrane Proteins; D003094:Collagen; D000111:Acetylcysteine