Effects of reactive oxygen species (ROS) modulators, TEMPOL and catalase, on methoxyacetaldehyde (MALD) -induced chromosome aberrations in Chinese hamster ovary (CHO)-AS52 cells.
Methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, has been shown to be clastogenic and mutagenic in CHO-AS52 cells. PCR-based-deletion screening of MALD induced CHO-AS52 mutants indicates that MALD induces large deletion mutation. Since MALD has an aldehyde as its reactive functional group, it can react with aldehyde oxidase to produce superoxide. The generation of these reactive oxygen species (superoxide, hydrogen peroxide and hydroxyl radical) may be the mechanism for genotoxicity of MALD. In the present study, TEMPOL and catalase which are ROS modulators were used to study the effects on MALD-induced chromosome damage in CHO-AS52 cells. The results showed that neither TEMPOL nor catalase can protect cells from MALD-induced chromosome aberrations. Therefore, the generation of reactive oxygen species may not be the primary mechanism of action of MALD.
Ratanavalachai
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TC
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand. mdbci010.chiangmai.ac.th
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D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D016466:CHO Cells; D002374:Catalase / Q000494:pharmacology*; D002869:Chromosome Aberrations; D006224:Cricetinae; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013723:Teratogens / Q000494:pharmacology*; D013997:Time Factors
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013723:Teratogens; C049826:2-methoxyacetaldehyde; D002374:Catalase; D000079:Acetaldehyde; C001803:tempol