Three-dimensional confocal microscopy of the lens.
A confocal microscopic technique is described to obtain optical sections from the in situ ocular lens of the rabbit and mouse. Optical sections were obtained with blue light through the full thickness of the lens epithelium, and of the anterior lenticular fibers. An image showing the structure of an anterior suture was obtained from the murine lens. A three-dimensional volume visualization of lenticular fibers from the anterior region of the rabbit lens was reconstructed from a stack of optical sections. These in situ microscopic observations of unstained, unfixed specimens correspond to previous studies using electron microscopy.
Masters
B R
BR
Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA.
D000818:Animals; D004847:Epithelial Cells; D007091:Image Processing, Computer-Assisted; D007908:Lens, Crystalline / Q000166:cytology*; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D011817:Rabbits