title: Self-association of human RAD52 protein.
abstract: The yeast RAD52 protein is required for both homologous DNA recombination and repair of DNA double-strand breaks. RAD52 can bind to the yeast RAD51 protein, which shares a functional similarity with the bacterial RecA protein. The gene encoding the human homolog of the yeast RAD52 protein shares significant N-terminus amino acid homology with the yeast RAD52 protein. Using a yeast two hybrid system and purified GST-RAD52 fusion protein, we demonstrate that the human RAD52 protein self-associates both in vivo and in vitro. The region of RAD52 required for its self-interaction, mapped here as amino acid residues 65-165, has significant homology with the yeast RAD52 (52% identity, and 89% similarity), suggesting the importance of self-association for RAD52's function.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, NM 87545, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Shen
authors.forename: S R
authors.initials: SR
authors.lastname: Peterson
authors.forename: J C
authors.initials: JC
authors.lastname: Comeaux
authors.forename: D
authors.initials: D
authors.lastname: Zastrow
authors.forename: R K
authors.initials: RK
authors.lastname: Moyzis
authors.forename: E M
authors.initials: EM
authors.lastname: Bradbury
authors.forename: D J
authors.initials: DJ
authors.lastname: Chen
mesh_terms: D001665:Binding Sites; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D051135:Rad51 Recombinase; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C495153:RAD51 protein, human; D051135:Rad51 Recombinase