title: Synovial fluid cell analysis.
abstract: Arthrocentesis has to be considered as a part of the clinical examination. A reasonable amount of aspirated synovial fluid is the best argument in favour of an objective articular disorder. Moreover some very simple evaluations are very helpful to make a diagnosis and to distinguish some particularities of rheumatic diseases. Such evaluations have to include both bacterial and synovial fluid analysis. Moreover, when performing synovial fluid analysis, a search for microcrystals is also performed. Haemarthrosis can easily be distinguished from a traumatic tap if the investigator is observing carefully the synovial fluid entering in the syringe. The diseases responsible for haemarthrosis differ with the age of patients: chondrocalcinosis, together with osteoarthrosis, is the most frequent aetiology in the elderly; disorders of haemostasis and synovial tumours are mostly observed in children and young adults. Paucicellular (< 1000 cells/mm3) synovial fluid is observed in different 'mechanical' disorders. In the case of purulent synovial fluid the primary diagnosis is septic arthritis. However, the most common aetiology is probably crystal-induced acute arthritis. Differential cell count analysis performed in case of 'inflammatory' (> 1000 or 2000 cells/mm3) synovial fluid usually shows a predominance of polymorphonuclear cells. However, high cellularity may sometimes be associated with a predominance of other cells, i.e. lymphocytes, monocytes, eosinophils. In this situation, such a simple evaluation (differential cell count analysis) is very helpful in making a diagnosis, e.g. eosinophilic arthritis, or to distinguish some particularities of rheumatic diseases, e.g. absence of cartilage breakdown in case of lymphocytic arthritis.
authors.affiliation: Department of Rheumatology, Hôpital Cochin, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Dougados
mesh_terms: D003460:Crystallization; D003937:Diagnosis, Differential; D006395:Hemarthrosis / Q000175:diagnosis / Q000209:etiology; D006801:Humans; D007592:Joint Diseases / Q000175:diagnosis*; D007958:Leukocyte Count; D010003:Osteoarthritis / Q000175:diagnosis; D010007:Osteochondritis / Q000175:diagnosis; D010020:Osteonecrosis / Q000175:diagnosis; D011677:Punctures / Q000295:instrumentation* / Q000379:methods; D012019:Reflex Sympathetic Dystrophy / Q000175:diagnosis; D013582:Synovial Fluid / Q000166:cytology* / Q000382:microbiology; D014947:Wounds and Injuries / Q000175:diagnosis