Disordered expression of glycolytic and gluconeogenic liver enzymes of juvenile visceral steatosis mice with systemic carnitine deficiency.
A quantitative study of the effect of carnitine deficiency on expression of glycolytic and gluconeogenic enzymes was performed using juvenile visceral steatosis mice which are systemically deficient in carnitine. The amounts of glucokinase and L-type pyruvate kinase mRNA were reduced in homozygotes, compared to heterozygotes and normal controls at 2 and 8 weeks. Liver-type phosphofructokinase, however, did not differ significantly. The abundance of fructose 1,6-bisphosphatase mRNA was unchanged at 2 and 8 weeks. The level of phosphoenolpyruvate carboxykinase mRNA was increased slightly at 2 weeks, but not at 8 weeks. A part of these changes could not be explained by the plasma glucose or insulin level. Carnitine administration restored the mRNA of these enzymes to normal levels. These results suggest that carnitine deficiency affects the expression of these liver enzymes.
Hotta
K
K
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Kuwajima
M
M
Ono
A
A
Nakajima
H
H
Horikawa
Y
Y
Miyagawa
J
J
Namba
M
M
Hanafusa
T
T
Horiuchi
M
M
Nikaido
H
H
Hayakawa
J
J
Saheki
T
T
Kono
N
N
Noguchi
T
T
Matsuzawa
Y
Y
D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D002331:Carnitine / Q000172:deficiency*; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000201:enzymology; D006597:Fructose-Bisphosphatase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics*; D005941:Glucokinase / Q000235:genetics / Q000378:metabolism; D005943:Gluconeogenesis; D006019:Glycolysis; D007003:Hypoglycemia / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008817:Mice, Mutant Strains; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D010732:Phosphofructokinase-1; D005941:Glucokinase; D011770:Pyruvate Kinase; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D002331:Carnitine