Expression of transforming growth factor-beta 1 in rat ventral prostate and Dunning R3327 PAP prostate tumor after castration and estrogen treatment.
BACKGROUND
In normal prostate, TGF-beta 1 is associated to castration induced apoptosis. Combined castration and estrogen treatment, but not castration alone, induces apoptosis in the Dunning R3327 PAP adenocarcinoma.


METHODS
TGF-beta 1 expression in rat ventral prostate (VP) and Dunning R3327 PAP tumor was studied after castration and estrogen treatment, using competitive RT-PCR, in situ hybridization and immunohistochemistry.


RESULTS
TGF-beta 1 mRNA level was 6 times higher in the tumor than in the VP. Combined castration and estrogen treatment increased TGF-beta 1 mRNA levels in the tumor from day 3, while castration did not. The TGF-beta 1 expression was located in the epithelial cells.


CONCLUSIONS
The Dunning R3327 PAP tumor contains high levels of TGF-beta 1, which are further increased by combined castration and estrogen treatment. However, since this increase is not apparent until day 3, TGF-beta 1 probably does not contribute to the known induction of apoptosis in the tumor at day 1 after combined castration and estrogen treatment.
Lindström
P
P
Department of Urology, Umeå university, Sweden.
Bergh
A
A
Holm
I
I
Damber
J E
JE
D000230:Adenocarcinoma / Q000378:metabolism* / Q000601:surgery*; D000818:Animals; D015152:Blotting, Northern; D004967:Estrogens / Q000627:therapeutic use*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000601:surgery; D009919:Orchiectomy; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000601:surgery*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
D004967:Estrogens; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta