Inhibitory effects of cocaine on NGF-induced neuronal differentiation: incomplete reversibility after a critical time period.
We extend our findings showing dose-dependent cocaine inhibition of differentiation in NGF-stimulated PC-12 cells without affecting cell viability by demonstrating that neurite extension is severely limited after 24 h, maximal effect is reached at 36 h and recovery is only partial. Cocaine metabolites lack these effects. A similar process may occur following human prenatal exposure, perhaps through cocaine-induced changes in gene expression or other intracellular signalling events.
Zachor
D A
DA
Department of Pediatrics, University of Alabama at Birmingham 35294-0017, USA.
Moore
J F
JF
Smoot
T M
TM
Percy
A K
AK
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D003042:Cocaine / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects*; D051381:Rats; D013997:Time Factors
D009414:Nerve Growth Factors; D003042:Cocaine