A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution.
We report the cloning of a 2.2 kb cDNA encoding a Na(+)-and Cl- dependent betaine/GABA (gamma-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe. Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.
Burnham
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CE
Division of Nephrology and Hypertension, University of Cincinnati College of Medicine, OH 45267-0585, USA. charles.burnham@ucbeh.san.uc.edu
Buerk
B
B
Schmidt
C
C
Bucuvalas
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001622:Betaine / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D050485:GABA Plasma Membrane Transport Proteins; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D017422:Sequence Analysis, DNA; D014981:Xenopus
D002352:Carrier Proteins; D018076:DNA, Complementary; D050485:GABA Plasma Membrane Transport Proteins; C072207:betaine plasma membrane transport proteins; D001622:Betaine