title: Evaluation of recent techniques for detection of red blood cell antibodies in sera of reference samples, patients, pregnant women, and blood donors.
abstract: The sensitivity of the low ionic strength solution antiglobulin test (LISS-AGT), polyethylene glycol antiglobulin test (PEG-AGT), low ionic strength solution solid-phase antiglobulin test (LISS-SPAT), gel low ionic strength solution antiglobulin test (GEL-LISS), and gel papain test (GEL-PAP) was compared in titration studies of 460 sera containing identified IgG alloantibodies. The GEL-PAP was 100% sensitive to detect Rh antibodies, whereas the PEG-AGT was the most sensitive to detect Kell, Duffy, Kidd, Ss, and rare blood group antibodies. The better performance of PEG-AGT was especially obvious with Kell, Duffy, and Ss antibodies (S = 100%). When the sensitivity of the LISS-AGT, PEG-AGT, GEL-LISS, and GEL-PAP was evaluated in different routines, the GEL-LISS showed to be more sensitive than PEG-AGT in the detection of clinically significant antibodies. These discrepant results showed that the performance of a technique may change when it is applied as a routine.
authors.affiliation: Centro de Hematologia e Hemoterapia, UNICAMP, Campinas, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: de Castilho
authors.forename: J
authors.initials: J
authors.lastname: Pellegrino
authors.forename: A P
authors.initials: AP
authors.lastname: Bechelli
authors.forename: P Y
authors.initials: PY
authors.lastname: Le Pennec
authors.forename: N F
authors.initials: NF
authors.lastname: Mendes
mesh_terms: D001782:Blood Donors; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D011247:Pregnancy / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D007518:Isoantibodies