Royal Society of Tropical Medicine and Hygiene meeting at Manson House, London, 14 December 1995. Enteropathogenic Escherichia coli--mucosal infection models.
The formation of attaching and effacing (A/E) lesions is central to the pathogenesis of enteropathogenic Escherichia coli (EPEC)-mediated disease in humans and Citrobacter rodentium-mediated transmissible colonic hyperplasia in mice. Closely related outer membrane proteins, known as intimins, are required for formation of the A/E lesion by both EPEC and C. rodentium. In this study we found similar ultrastructural damage in small intestinal biopsies from an EPEC-infected child and large bowel specimens from C. rodentium-infected mice. The C. rodentium-infected large bowel biopsies revealed massive hyperplastic reactions and the infected human small intestinal biopsies showed an increase in total crypt cell number and mitotic index. EPEC-infected small intestinal organ cultures revealed bacteria adhering in a localized pattern and evidence of A/E lesions. Covaspheres coated with a biologically active cell-binding domain of intimin also adhered to cells in a localized fashion but did not induce the characteristic A/E lesions.
Frankel
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Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
Phillips
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Hicks
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Dougan
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D018829:Adhesins, Bacterial; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology; D001706:Biopsy; D002352:Carrier Proteins; D002648:Child; D016971:Citrobacter freundii / Q000472:pathogenicity; D004195:Disease Models, Animal; D004756:Enterobacteriaceae Infections / Q000473:pathology; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000473:pathology*; D029968:Escherichia coli Proteins; D005260:Female; D006801:Humans; D007420:Intestine, Large / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D007421:Intestine, Small / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli