Apoptosis induced by selenium in human glioma cell lines.
Several studies have shown that selenium can inhibit tumorigenesis in tissues. However, little is known about the mechanism and the effect of selenium on DNA, especially in brain tumor cells. In this study we examined the biological effect of selenium on human glioma cell lines (A172 and T98G). Selenium exhibited an antiproliferative effect on these cell lines (and induced the typical ladder pattern of DNA fragmentation commonly found in apoptosis), which were prevented by catalase. Few effects of selenium on NT14 fibroblasts were found. These findings demonstrate that selenium may induce, by apoptosis, cell death of human glioma cell lines, which are resulting from free radical oxygen forming.
Zhu
Z
Z
Kyoto University, Graduate School of Medicine, Department of Social Medicine, Japan.
Kimura
M
M
Itokawa
Y
Y
Aoki
T
T
Takahashi
J A
JA
Nakatsu
S
S
Oda
Y
Y
Kikuchi
H
H
D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D002374:Catalase / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D004586:Electrophoresis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008856:Microscopy, Fluorescence; D012643:Selenium / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D002374:Catalase; D012643:Selenium