Activation of neutrophil-like HL-60 cells by prostaglandin E2.
The effect of prostaglandins on neutrophil activation has been studied using the human promyelocytic leukemic cell line HL-60, differentiated with dimethyl sulfoxide (DMSO). Prostaglandin E(2) (PGE(2)) directly stimulated HL-60 cell aggregation with an EC(50) value of 30 nM. Studies with prostanoid receptor-selective agonists suggest that the activation of HL-60 cells by PGE(2) was mediated via the EP(2) receptor. Human neutrophils did not aggregate in response to PGE(2), but PGE(2) inhibited the N-formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated increase in intracellular calcium ([Ca(2+)i]) in both neutrophils and HL-60 cells. In contrast with the aggregation response to FMLP the aggregation response of HL-60 cells to PGE(2) was independent of extracellular calcium and did not involve mobilization of intracellular calcium.
Wise
H
H
Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT Hong Kong.
Xie
Z H
ZH
D000527:Alprostadil / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002449:Cell Aggregation / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000494:pharmacology; D018078:Receptors, Prostaglandin E / Q000819:agonists / Q000378:metabolism
D011453:Prostaglandins; D018078:Receptors, Prostaglandin E; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000527:Alprostadil; D015232:Dinoprostone; D002118:Calcium