Genotyping of hepatitis C virus isolates from Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis of 5' noncoding region.
We have genotyped the 5' noncoding region of hepatitis C virus in Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis. Of 50 patients, 15 had the expected 268-bp amplicon by reverse transcription-PCR. Specificity of the amplicons was confirmed by Southern hybridization. Restriction analysis of the amplicons showed the pattern for genotype 4 (common in the Middle East).
Matar
G M
GM
Department of Microbiology and Immunology, Faculty of Medicine, American University of Beirut, New York, New York 10022, USA. gmatar@layla.aub.ac.lb
Sharara
H M
HM
Abdelnour
G E
GE
Abdelnoor
A M
AM
D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007861:Lebanon; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004279:DNA, Viral