Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1.
From a murine B-cell cDNA-library we have cloned a cDNA encoding the murine B-cell specific coactivator mBob1. The protein is the murine homologue to the recently described human coactivator Bob1 (hBob1), also referred to as OBF-1 or OCA-B. We have also characterized the genomic mBob1 clone. Analysis of its intron-exon structure has allowed identification of a C-terminal splice variant. mBob1 is B-cell restricted, and is found in all B-cell lines representing different stages of B-cell differentiation. mBob1 interacts with the octamer transcription factors Oct-1 and Oct-2 and stimulates transcription mediated by these factors.
Knoepfel
L
L
Institut für Molekularbiologie II der Universität Zürich, Switzerland.
Georgiev
O
O
Nielsen
P
P
Schaffner
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D015723:Gene Library; D051863:Host Cell Factor C1; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D051381:Rats; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C091546:POU2AF1 protein, human; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; C490424:Pou2af1 protein, mouse; C494166:Pou2f1 protein, mouse; C494168:Pou2f1 protein, rat; C489206:Pou2f2 protein, mouse; C489205:Pou2f2 protein, rat; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA