title: Expression of c-fos, jun D and pp60c-src+ mRNAs in the developing and grafted rat striatum.
abstract: Expression of the mRNAs of the proto-oncogenes pp60c-src+, c-fos and jun D were studied using in-situ hybridisation histochemistry in the developing striatum and in striatal grafts. The temporal patterns of mRNA expression were monitored in the striatum of the normal developing rat from the 12th day of gestation (E12) to 10 days postnatally, and were compared to the changes in gene expression observed in E13-E14 primordial striatal tissue grafts 7, 15 and 30 days after implantation in the ibotenic acid-lesioned striatum of adult rats. During development, all three proto-oncogenes were most highly expressed just before birth, at E19. Striatal expression of all three proto-oncogenes was markedly reduced after birth and remained at a low level through to adulthood. A different mode of expression was observed in the transplanted striatum which was unique to each particular gene. jun D and pp60c-src+ were expressed for a longer time period in the grafted primordial cells than in normal development, whereas no c-fos expression could be detected in the grafts. These results suggest that transplantation of embryonic neural cells into the host brain may affect the normal developmental regulation of such cells and their expression of some proto-oncogenes.
authors.affiliation: MRC Cambridge Centre for Brain Repair, University of Cambridge, UK.
authors.forename: N S
authors.initials: NS
authors.lastname: Haque
authors.forename: J M
authors.initials: JM
authors.lastname: Fernandez
authors.forename: E
authors.initials: E
authors.lastname: Mayer
authors.forename: R P
authors.initials: RP
authors.lastname: Heavens
authors.forename: S B
authors.initials: SB
authors.lastname: Dunnett
authors.forename: D J
authors.initials: DJ
authors.lastname: Sirinathsinghji
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation; D017690:Cell Transplantation; D003342:Corpus Striatum / Q000187:drug effects / Q000196:embryology / Q000254:growth & development / Q000378:metabolism* / Q000637:transplantation; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D016332:Fetal Tissue Transplantation; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D016762:Genes, fos; D016758:Genes, jun; D005865:Gestational Age; D007051:Ibotenic Acid / Q000633:toxicity; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D007051:Ibotenic Acid; D016392:Proto-Oncogene Proteins pp60(c-src)