Development-dependent localization of nuclear antigens in growing mouse oocytes.
We have analyzed the distribution of nuclear and nucleolar proteins during the period of oocyte's growth. Oocytes were isolated mechanically or enzymatically from ovaries of juvenile mice of various ages (from 1 to 28 days after birth). Small nuclear ribonucleoproteins (snRNPs), the splicing factor SC-35, and a protein linked to cell proliferation (p-120) were detected by indirect immunofluorescence. snRNP distribution is consistent with the prophase state of oocyte's nuclei, while SC-35 (and p-120) exhibit a "speckled" distribution throughout the entire period of growth. The number of speckles (or foci) appears maximal around 10 days after birth, i.e., in the period of maximal transcriptional activity, and is sensitive to alpha-amanitin treatment. On the other hand, the immunofluorescent distribution of of nucleolin and p-103 (a nucleolar marker of the granular component) is compared to the ultrastructural distribution of the granular component analyzed by electron microscopy on oocytes of the same age.
Borsuk
E
E
Department of Embryology, University of Warsaw, Poland.
Vautier
D
D
Szöllösi
M S
MS
Besombes
D
D
Debey
P
P
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D034961:Antigens, Nuclear; D051379:Mice; D009687:Nuclear Proteins / Q000276:immunology*; D009865:Oocytes / Q000276:immunology*; D010449:Peptide Mapping; D011496:Protein Methyltransferases; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear / Q000276:immunology*; D000068103:Serine-Arginine Splicing Factors; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D034961:Antigens, Nuclear; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; C499372:SRSF2 protein, mouse; D000068103:Serine-Arginine Splicing Factors; C512901:Nop2 protein, mouse; D011496:Protein Methyltransferases