Role of galectin-1 in the developing mouse olfactory system.
Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the present study, the mouse olfactory neuron cell line 4.4.2 was used to examine the role of galectin-l in neurite outgrowth in vitro. Recombinant galectin-l has neurite outgrowth-promoting activity when used as a substrate for 4.4.2 cells. When either galectin-1 or lactose was added to the culture media, the neurite outgrowth-promoting activity was abolished. These results demonstrate that galectin-1 can modulate neurite growth in vitro. The in vivo role of galectin-1 was investigated by examining the topographical organization of the olfactory pathway in mice carrying a null mutation for galectin-1. Using Dolichos biflorus agglutinin as a convenient histochemical marker of a subpopulation of primary sensory olfactory neurons which project topographically to the dorsomedial olfactory bulb, we show an aberrant topography of olfactory axons in the null mutants. A subset of primary sensory olfactory axons failed to project to their correct target sites in the caudal olfactory bulb. These data indicate that galectin-1 is involved in the growth and/or guidance of primary sensory olfactory axons between the nasal cavity and the olfactory bulb. This is the first demonstration that a lectin has neurite outgrowth-promoting activity and plays a role in neuronal pathfinding in the mammalian nervous system.
Puche
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Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
Poirier
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F
Hair
M
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Bartlett
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PF
Key
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B
D000818:Animals; D001369:Axons / Q000502:physiology*; D002999:Clone Cells; D037483:Galectin 1; D006388:Hemagglutinins / Q000502:physiology*; D037102:Lectins / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000502:physiology; D009830:Olfactory Bulb / Q000002:abnormalities / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009833:Olfactory Pathways / Q000002:abnormalities / Q000254:growth & development*; D011994:Recombinant Proteins
D037483:Galectin 1; D006388:Hemagglutinins; D037102:Lectins; D011994:Recombinant Proteins