The production of superoxide anion and nitric oxide by cultured murine leukocytes and the accumulation of TNF-alpha in the conditioned media is inhibited by taurine chloramine.
Taurine chloramine (Tau-Cl) inhibits production of nitric oxide (NO) by activated peritoneal macrophages and attenuates accumulation of tumor necrosis factor-alpha (TNF-alpha) in the culture media, similar to that previously reported for activated RAW 264.7 cells. In addition, the effect of Tau-Cl and taurine on superoxide anion (O2-) production in murine peritoneal exudate polymorphonuclear leukocytes (PMN) was examined. Tau-Cl inhibited O2- production in a manner that was dose-dependent and reversible. Taurine also inhibited O2- production by stimulated PMN, but at higher concentrations and to a lesser extent than Tau-Cl. The effects of taurine on O2- production was attributed to the in vitro formation of Tau-Cl catalyzed by PMN associated halide-dependent myeloperoxidase. In contrast, production of NO by activated peritoneal macrophages and accumulation of TNF-alpha in the media was inhibited by Tau-Cl while taurine was without effect. These data lend support to the notion that Tau-Cl may participate in the inflammatory response by modulating production of inflammatory mediators.
Kim
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C
Department of Immunology, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314, USA.
Park
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Quinn
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MR
Schuller-Levis
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G
D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D013654:Taurine; D009569:Nitric Oxide; C043410:N-chlorotaurine