title: Treatment with recombinant bovine interferon-tau in utero attenuates secretion of prostaglandin F from cultured endometrial epithelial cells.
abstract: Endometrial oxytocin receptors and total production of PGF by endometrial epithelial cells were measured in 10 cyclic cows after intrauterine injections of recombinant bovine interferon-tau plus BSA or BSA alone. Cows received twice daily injections (via intrauterine catheters) of 200 micrograms of recombinant bovine interferon-tau plus 1.3 mg of BSA (n = 5) or 1.5 mg of BSA (n = 5) from d 14 to 17 after estrus. On d 17, the reproductive tracts of each cow was removed at slaughter, and endometrial epithelial cells were cultured with 0, 2, or 50 ng/ml of recombinant bovine interferon-tau. After 24 h, oxytocin (2 x 10(-7) M) was added to one-half of the culture wells, and the medium was sampled at 0, 30, and 90 min for analysis of total PGF (PGF plus 13, 14-dihydro-15-keto-PGF2 alpha). In vivo treatment with recombinant bovine interferon-tau + BSA reduced total secretion of PGF in culture (1.49 +/- 0.06 vs. 2.80 +/- 0.07 ng/micrograms of DNA), but did not block the oxytocin-induced stimulation in total secretion of PGF. In vitro treatment of cells with recombinant-bovine interferon-tau did not decrease basal secretion of total PGF. Oxytocin receptor binding at d 17 was low in both treatments but slightly attenuated in the group treated with recombinant bovine interferon-tau.
authors.affiliation: Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611-0920, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Meyer
authors.forename: G D
authors.initials: GD
authors.lastname: Desnoyers
authors.forename: B
authors.initials: B
authors.lastname: Oldick
authors.forename: W W
authors.initials: WW
authors.lastname: Thatcher
authors.forename: M
authors.initials: M
authors.lastname: Drost
authors.forename: T K
authors.initials: TK
authors.lastname: Schalue
authors.forename: R M
authors.initials: RM
authors.lastname: Roberts
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D010121:Oxytocin / Q000378:metabolism / Q000494:pharmacology; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011460:Prostaglandins F / Q000378:metabolism*; D018045:Receptors, Oxytocin / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014599:Uterus / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; D011460:Prostaglandins F; D018045:Receptors, Oxytocin; D011994:Recombinant Proteins; C020947:interferon tau; D012710:Serum Albumin, Bovine; D010121:Oxytocin