title: katG mutations in isoniazid-resistant Mycobacterium tuberculosis isolates recovered from Finnish patients.
abstract: katG and inhA genes from isoniazid-resistant Mycobacterium tuberculosis strains isolated in Finland were examined by PCR or sequencing. By PCR, katG was not detected in 3 of 54 strains. Sequencing of katG from 13 strains showed small point mutations or insertions; a previously described mutation causing a Ser-to-Thr change at position 315 was found in 4 strains, and there were nine new missense mutations of katG. A 209-bp segment of inhA from 17 strains was sequenced, but no mutations were observed. This result indicates that different mutations prevail in different geographical areas.
authors.affiliation: National Public Health Institute, Mycobacterial Reference Laboratory, Turku, Finland. harmar@utu.fi
authors.forename: H J
authors.initials: HJ
authors.lastname: Marttila
authors.forename: H
authors.initials: H
authors.lastname: Soini
authors.forename: P
authors.initials: P
authors.lastname: Huovinen
authors.forename: M K
authors.initials: MK
authors.lastname: Viljanen
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005387:Finland; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid