Tracing the auditory pathways to electrophysiologically characterized neurons with HRP and Fos double-labeling technique.
By combining HRP histochemistry with Fos immunocytochemistry, we demonstrate in this study that electrophysiologically characterized auditory neurons can be double-labeled with HRP and Fos after iontophoretic injection of HRP into the recording site. Neurons which projected fibers to the recording site were labeled with HRP and were Fos-like immunoreactive. This double-labeling technique in combination with electrophysiological recording offers the possibility to determine the fiber projections between sound-activated neurons which are identified either electrophysiologically and/or immunocytochemically.
Qian
Y
Y
Division of Biological Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
Wu
M
M
Jen
P H
PH
D000818:Animals; D001306:Auditory Pathways / Q000502:physiology; D002685:Chiroptera / Q000502:physiology*; D004594:Electrophysiology; D006309:Hearing / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D009475:Neurons, Afferent / Q000737:chemistry / Q000502:physiology*; D011149:Pons / Q000737:chemistry / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D016760:Proto-Oncogene Proteins c-fos; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate