Effect of ligand orientation on hepatocyte attachment onto the poly(N-p-vinylbenzyl-o-beta-D-galactopyranosyl-D-gluconamide) as a model ligand of asialoglycoprotein.
The orientation effect of galactose ligand on hepatocyte attachment was investigated. Poly(N-p-vinylbenzyl-o-beta-D-galactopyranosyl-D-gluconamide )(PVLA), a beta-galactose-carrying styrene homopolymer, was used as a model ligand for the asialoglycoprotein receptors on hepatocytes. PVLA was transferred onto the poly(gamma-benzyl L-glutamate) (PBLG) or PBLG/poly(ethylene glycol) (PEG)PBLG Langmuir-Blodgett (LB) films as the monolayer level. The dichroic fluorescence values of the confocal microscope indicated that the PVLA transferred onto the LB films was located with a preferential orientation of its molecular axes with regard to the direction of the alpha-helix of polypeptide. Hepatocyte recognized well-oriented galactose moieties of the surface of PVLA through asialoglycoprotein receptors.
Cho
C S
CS
Department of Polymer Engineering, Chonnam National University, Kwangju, Korea.
Goto
M
M
Kobayashi
A
A
Kobayashi
K
K
Akaike
T
T
D000818:Animals; D016880:Anisotropy; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D003287:Contrast Media / Q000737:chemistry; D005260:Female; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D018613:Microscopy, Confocal; D011092:Polyethylene Glycols / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D003287:Contrast Media; D011137:Polystyrenes; D011956:Receptors, Cell Surface; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose