Xrcc-1 expression during male meiosis in the mouse.
XRCC1 is involved in DNA strand-break repair, homologous recombination, and sister chromatid exchange and is expressed as a low-abundance mRNA with elevated expression in testis. The purpose of this study was to determine whether specific spermatogenic cell types have elevated Xrcc-1 expression and whether expression levels change in the testis with increased age. Northern blot analysis of mRNA prepared from testes of 15-, 25-, and 60-day-old mice revealed a single hybridizing band of 2.2 kb. Quantitative RNase protection assays revealed no changes in the level of Xrcc-1 expression in testis relative to DNA content among 6-, 12-, 18-, 24-, or 28-mo-old mice. Finally, reverse transcription coupled polymerase chain reaction amplification results demonstrated that Xrcc-1 expression is most abundant in pachytene spermatocytes and round spermatids with low expression in Sertoli cells, types A and B spermatogonia, preleptotene spermatocytes, and leptotene plus zygotene spermatocytes. The relatively abundant Xrcc-1 expression in pachytene spermatocytes and round spermatids suggests that Xrcc-1 is involved in DNA strand-break repair associated with meiotic recombination in addition to its previously implicated role in strand-break repair associated with base excision repair.
Walter
C A
CA
Department of Cellular and Structural Biology, University of Texas Health Science, Center at San Antonio 78284-7762, USA. walter@uthscsa.edu
Trolian
D A
DA
McFarland
M B
MB
Street
K A
KA
Gurram
G R
GR
McCarrey
J R
JR
D000375:Aging / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000032:analysis; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism; D000076105:X-ray Repair Cross Complementing Protein 1
D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619352:Xrcc1 protein, mouse; D012313:RNA; D004247:DNA; D012260:Ribonucleases