title: The glomerular filtration rate during pregnancy: saline infusion enhances the glomerular filtration rate in the pregnant rat.
abstract: The glomerular filtration rate (GFR) of pregnant rats is generally believed to exceed non-pregnant values. This notion is primarily based upon standard insulin clearances. However, the insulin clearance requires continuous infusion of insulin usually dissolved in saline. Since saline infusion per se in pregnancy may influence the GFR, in the present study the effect of saline infusion upon the GFR in pregnant as compared with cyclic rats was investigated using various methods. The standard insulin clearance was compared using the standard 51Cr-EDTA method which does not require saline infusion. Clearance of insulin dissolved in glucose (5% in distilled water) instead of saline was also tested, while the 51Cr-EDTA method was employed using additional fluid infusion with either saline or 5% glucose in distilled water in an identical manner as compared with the insulin method. The GFR was also studied in conscious rats using 51Cr-EDTA clearance with and without fluid infusion. The distribution volume of 51Cr-EDTA was measured in nephrectomized rats (pregnant and cyclic) with and without saline or glucose infusions. The results show a significant increase of the GFR in pregnant rats as compared with cyclic rats only when saline was infused during the measurement; thus, GFR measurements without fluid infusion or replacement of saline by glucose during the measurements did not show a significant increase of GFR in pregnant rats. The volume of distribution per gram body weight of 51Cr-EDTA after saline infusion, but not after glucose infusion, was significantly increased as compared with the values obtained without additional infusion. It is concluded that the increase of the GFR seen in pregnant rats when either the 51Cr-EDTA method or the insulin method is accompanied by saline loading is rather due to infusion of saline in the pregnant animal and not a result of the pregnant condition per se.
authors.affiliation: Department of Pathology, University of Groningen, The Netherlands.
authors.forename: M M
authors.initials: MM
authors.lastname: Faas
authors.forename: G A
authors.initials: GA
authors.lastname: Schuiling
authors.forename: P A
authors.initials: PA
authors.lastname: Klok
authors.forename: N
authors.initials: N
authors.lastname: Valkhof
authors.forename: W W
authors.initials: WW
authors.lastname: Bakker
mesh_terms: D000818:Animals; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000493:pharmacokinetics; D004971:Estrus / Q000502:physiology*; D005260:Female; D005919:Glomerular Filtration Rate; D007263:Infusions, Parenteral; D007444:Inulin / Q000493:pharmacokinetics; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008657:Metabolic Clearance Rate; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D002860:Chromium Radioisotopes; D012965:Sodium Chloride; D007444:Inulin; D004492:Edetic Acid