title: Comparison of HPLC and GC-MS for measurement cocaine and metabolites in human urine.
abstract: High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GC-MS) for quantitation of cocaine, benzoylecgonine, norcocaine, and cocaethylene in urine. Calibration standards were prepared in human urine, and bupivacaine was added as the internal standard for quantitation. After solid-phase extraction, the reconstituted samples were divided into aliquots for analysis by HPLC and GC-MS. The analytical performance of the two methods were compared with regard to sensitivity, precision, and dynamic range. Results of GC-MS and HPLC analyses of nine urine specimens previously confirmed as positive for benzoylecgonine were compared. Analytical results by HPLC were comparable to GC-MS. Therefore, for many laboratories, HPLC is a useful alternative to GC-MS for measuring cocaine and metabolites in urine.
authors.affiliation: Department of Pharmaceutics, University of Florida, Gainesville 32610, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Phillips
authors.forename: I R
authors.initials: IR
authors.lastname: Tebbett
authors.forename: R L
authors.initials: RL
authors.lastname: Bertholf
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*
chemical_list: C008671:norcocaine; C005618:benzoylecgonine; C066444:cocaethylene; D003042:Cocaine