Inhibition of Raf/MAPK signaling in Xenopus oocyte extracts by Raf-1-specific peptides.
Raf-1 is an upstream element of the mitogen-activated protein kinase (MAPK) pathway which leads to cell proliferation and differentiation. In this study Raf-1 derived peptides comprising the conserved amino acid residues Arg89 and Ser259, involved in binding of activated Ras and 14-3-3 proteins, respectively, were shown to interfere with MAPK activation in extracts from immature Xenopus oocytes. Lipids prepared from oocyte extracts can stimulate MAPK in a Ras- and protein kinase C-independent manner. This lipid-induced MAPK activation is blocked by a Raf-1 derived peptide comprising Ser259.
Radziwill
G
G
Institut für Medizinische Virologìe, Universität Zürich, Gloriastrasse, Switzerland.
Steinhusen
U
U
Aitken
A
A
Moelling
K
K
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D002457:Cell Extracts; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf; D015398:Signal Transduction; D014446:Tyrosine 3-Monooxygenase; D014981:Xenopus; D018631:ras Proteins / Q000378:metabolism
D048948:14-3-3 Proteins; D002457:Cell Extracts; D010455:Peptides; D011506:Proteins; D011518:Proto-Oncogene Proteins; D014446:Tyrosine 3-Monooxygenase; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins