title: Functional expression of CD11a on CD8+ cells is suppressed in regional lymph nodes with cancer involvement in patients with gastrointestinal carcinoma.
abstract: BACKGROUND
  To evaluate the immunologic activity of regional lymph nodes, the phenotype of lymphocytes and the functional expression of cell adhesion molecules (CAMs) on lymph node lymphocytes (LNL-: uninvolved, LNL+: involved) were investigated in patients with gastrointestinal carcinoma.
  
  
  METHODS
  The lymphocyte subpopulation and the expression of CD11a, CD44, and CD29 on CD4+ and CD8+ cells in peripheral blood lymphocytes (PBL), LNL- and LNL+ derived from 37 patients with gastrointestinal carcinoma were studied. In addition, the adherence of CD8+ cells to ICAM-1 which reflects the adhesive function of CD11a, was examined, and changes in this adherence were studied by experimental coculture with cancer cells (DLD-1).
  
  
  RESULTS
  Although there were no differences in the overall proportion of T cells between the groups, CD8+ cells and CD16+ cells were considerably diminished in LNL+. The expression of CD11a and CD29 on CD4+ and CD8+ cells was significantly lower in LNL than in PBL, whereas the expression of CD44 showed no significant differences. The expression levels of these CAMs were almost the same in LNL- and LNL+. Only CD11a expression on CD8+ cells in LNL+ was significantly lower than that in LNL- (P < 0.005). The adherence of CD8+ cells in LNL+ to ICAM-1 was lower than that in PBL and LNL-, and was extremely enhanced by experimental coculture with cancer cells (DLD-1).
  
  
  CONCLUSIONS
  These data indicate that the functional expression of CD11a (LFA-1) on CD8+ T cells is suppressed in cancer-involved regional lymph nodes in patients with gastrointestinal carcinoma.
authors.affiliation: First Department of Surgery, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shinkal
authors.forename: J
authors.initials: J
authors.lastname: Kitayama
authors.forename: W
authors.initials: W
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Muto
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000276:immunology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005770:Gastrointestinal Neoplasms / Q000097:blood / Q000276:immunology*; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000302:isolation & purification*; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010641:Phenotype
chemical_list: D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1