title: Formation of O6-ethylguanine in spermatogonial DNA of adult Syrian golden hamster by intraperitoneal injection of diethylnitrosamine.
abstract: 17-Week-old Syrian golden hamsters received a single intraperitoneal injection of diethylnitrosamine (DEN) at a dose of 100 mg/kg body weight. The DNA alkylation product O6-ethylguanine was formed in the spermatogonia. The data demonstrate that DEN can pass the blood-testis barrier and be metabolized in the spermatogonia to yield an alkylating derivative or that externally activated metabolites themselves can pass the barrier.
authors.affiliation: Institute of Experimental Pathology, Hannover Medical School, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Kamino
authors.forename: F
authors.initials: F
authors.lastname: Seiler
authors.forename: M
authors.initials: M
authors.lastname: Emura
authors.forename: J
authors.initials: J
authors.lastname: Thomale
authors.forename: M F
authors.initials: MF
authors.lastname: Rajewsky
authors.forename: U
authors.initials: U
authors.lastname: Mohr
mesh_terms: D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism*; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D008297:Male; D008647:Mesocricetus; D013093:Spermatogonia / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D018736:DNA Adducts; D004052:Diethylnitrosamine; C007673:6-ethylguanine; D006147:Guanine; D004247:DNA