Inhibitory effect of atractylon on tert-butyl hydroperoxide induced DNA damage and hepatic toxicity in rat hepatocytes.
Atractylon, a main sesquiterpenic constituent of Atractylodes rhizomes, was studied for the mechanism of its inhibitory effects on the tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity and lipid peroxidation in primary culture of rat hepatocytes. In the preliminary study, atractylon showed an effective antioxidant property tested by its capacity for quenching 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Further investigations showed that atractylon at the concentrations of 0.01, 0.1 and 1.0 mg/ml decreased the formation of malondialdehyde (MDA), leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) and repair synthesis of DNA induced by 30-min treatment of t-BHP (1.5 mM) in primary cultured rat hepatocytes. Addition of atractylon also attenuated the genotoxicity of t-BHP evaluated by unscheduled DNA synthesis. The sum of the results suggested that the protective effect of atractylon against oxidative stress induced by t-BHP is via its ability to quench free radicals.
Hwang
J M
JM
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
Tseng
T H
TH
Hsieh
Y S
YS
Chou
F P
FP
Wang
C J
CJ
Chu
C Y
CY
D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010545:Peroxides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000627:therapeutic use*; D013778:Tetrazolium Salts / Q000032:analysis; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; C046196:atractylon; D020122:tert-Butylhydroperoxide