The prepubertal ontogeny of luteinizing hormone releasing hormone-like immunoreactivity in the diencephalon and telencephalon of the male Meishan pig brain.
Luteinizing hormone releasing hormone (LHRH) is a decapeptide that regulates reproductive function and behaviors in mammalian species. Because of the importance of proper reproductive function and efficiency in agricultural species, we have investigated the prepubertal ontogeny of LHRH-like immunoreactivity (IR) in the male Meishan pig. The Meishan is a Chinese breed known for reproductive traits including increased litter size and precocious puberty, but slow growth and obesity. Brains of animals from gestational day (g) 30, 50, 70, 90 and 110 and postnatal day (pn) 1, 10, 20 and 50 (duration of pregnancy averaged 114 days) were processed using a standard immunohistochemical technique utilizing a commercially available rabbit anti-LHRH antibody. LHRH-IR in cell bodies and fibers was detected at g30 entering the brain via the terminal nerve and in the septal region of the basal telencephalon. The number of immunoreactive cells increased at g50 and cells were localized primarily to the septum, organum vasculosum of the lamina terminalis, preoptic area and lateral hypothalamus, whereas immunoreactive fibers were present throughout the septum and hypothalamus and had reached the median eminence. The density and distribution of immunoreactive fibers increased by g70 and g90, but did not change dramatically from g90 to pn50. These results indicate that LHRH may be present in the Meishan brain earlier during development and fibers containing LHRH-IR appear to reach the median eminence earlier than previously reported for the domestic pig. These results suggest a breed difference in the ontogeny of reproductive control systems in the pig. Future studies utilizing direct comparisons between domestic and Chinese breeds will be required to investigate the apparent differences in the ontogeny of LHRH-containing systems in the pig.
Pearson
P L
PL
Department of Veterinary Anatomy, Iowa State University, Ames 50011, USA.
Anderson
L L
LL
Jacobson
C D
CD
D000818:Animals; D004027:Diencephalon / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D005865:Gestational Age; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D012741:Sexual Maturation / Q000502:physiology*; D013552:Swine; D013687:Telencephalon / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development
D007987:Gonadotropin-Releasing Hormone