Systematic analytic procedure for identification of unknown medicaments in biological material.
The presented method has been used in toxicological practice for identification of unknown medicaments in biological material longer than fifteen years with good results. This method described as TLC/CR-screening is a combination of thin layer chromatography (TLC) with a system of colour reactions (CR-screening), the principle being evaluation of characteristic features (qualities) of each detected unknown substance (parent medicament form or its metabolite) from three points of view: the type of ionization during the extraction, the mobility in basic TLC-system in comparison with five reference standards, the reactivity with system reagents for detection. The properties of each detected substance expressed by adequate symbols are put together into three component codes, under which corresponding standards of medicaments and/or their metabolites can be found in the library of code system.
Vecerková
J
J
Institute for Toxicology and Forensic Chemistry, Charles University, Praha, Czech Republic.
D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity*; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D004340:Drug Contamination; D006801:Humans; D014116:Toxicology / Q000379:methods
D001672:Biocompatible Materials