title: GT3 synthesis in the proximal Golgi occurs in a compartment different from those for GD3 and GM3 synthesis.
abstract: Previous studies from this laboratory have shown that synthesis of GT3, the precursor of c series gangliosides, occurs in proximal Golgi compartments, as has been shown for the synthesis of GM3 and GD3, the precursors of a and b series gangliosides, respectively. In this work we studied whether the synthesis of GM3, GD3, and GT3 occurs in the same or in different compartments of the proximal Golgi. For this, we examined in retina cells (a) the effect of monensin, a sodium ionophore that affects mostly the trans Golgi and the trans Golgi network function, on the metabolic labeling of glycolipids from [3H]Gal by cultured cells from 7- and 10-day chick embryos and (b) the labeling in vitro of endogenous glycolipids of Golgi membrane preparations from 7-day embryos incubated with UDP-[3H]Gal. In (a), 1 microM monensin produced a twofold accumulation of radioactive glucosylceramide and a decrease to approximately 50 and 20% of total ganglioside labeling in 7- and 10-day cells, respectively. At both ages, monensin produced a threefold accumulation of radioactive GM3 and an inhibition of > 90% of GT3, GM1, GD1a, and GT1b synthesis. GD3 synthesis was inhibited approximately 30 and 70%, respectively, in 7- and 10-day cells. In (b), > 80% of the [3H]Gal was incorporated into endogenous glucosylceramide to form radioactive lactosylceramide. About 90% of [3H]Gal-labeled lactosylceramide was converted into GM3, and most of this in turn into GD3 when unlabeled CMP-NeuAc was also present in the incubation system. Under the same conditions, however, < 5% of labeled GD3 was converted into GT3. Golgi membranes incubated with CMP-[3H]NeuAc incorporated approximately 20% of [3H]NeuAc into endogenous GT3, and this percentage was not affected by 1 microM monensin. These results indicate that synthesis of GT3 is carried out in a compartment of the proximal Golgi different from those for lactosylceramide, GM3, and GD3 synthesis. Results from the experiments with monensin point to the cis/medial Golgi as the main compartment for coupled synthesis of lactosylceramide, GM3, and GD3 and to the trans Golgi as the main compartment for synthesis of GT3.
authors.affiliation: CIQUIBIC (UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
authors.forename: V M
authors.initials: VM
authors.lastname: Rosales Fritz
authors.forename: M K
authors.initials: MK
authors.lastname: Maxzúd
authors.forename: H J
authors.initials: HJ
authors.lastname: Maccioni
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D005679:G(M3) Ganglioside / Q000096:biosynthesis*; D005732:Gangliosides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007790:Lactosylceramides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D008985:Monensin / Q000494:pharmacology; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
chemical_list: D005679:G(M3) Ganglioside; D005732:Gangliosides; D007790:Lactosylceramides; D014531:Uridine Diphosphate Galactose; C026226:ganglioside, GD3; C047989:trisialosyllactosylceramide; D008985:Monensin