Dual CD38 and DNA staining of plasma cells in monoclonal gammopathies attained by a rapid, non-wash and automated method.
Dual antigenic and DNA analysis are a prerequisite in order to study DNA content and cell cycle distribution accurately and identify, while studying them separately, neoplastic cells in the mixture of tumour samples with a high proportion of normal cells or when residual normal cells are highly proliferative. We describe a method for the simultaneous detection of surface CD38 antigen and study, by flow cytometry, of DNA content in the bone marrow of patients with a monoclonal gammopathy. This standardized, easy-to-perform, and automated flow cytometric non-wash technique allows dual antigen and DNA staining in less than 30 min. The method identifies bone marrow plasma cells and separately calculates their cell cycle distribution by means of a double staining technique for CD38 surface antigen and DNA content.
Gómez-Arbonés
X
X
Haematology Department, Arnau de Vilanova University Hospital, University of Lleida, Spain.
Gallart
M A
MA
Macià
J
J
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis*; D001331:Automation; D001856:Bone Marrow Examination / Q000295:instrumentation / Q000379:methods*; D002453:Cell Cycle; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008562:Membrane Glycoproteins; D009101:Multiple Myeloma / Q000473:pathology; D009699:N-Glycosyl Hydrolases / Q000032:analysis*; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000473:pathology; D010265:Paraproteinemias / Q000473:pathology*; D010950:Plasma Cells / Q000737:chemistry* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D004247:DNA; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1