title: Age- and strain-related changes in tissue transglutaminase activity in murine macrophages: the effects of inflammation and induction by retinol.
abstract: We describe differences in the activity of tissue transglutaminase (TGase) between resident and inflammatory mouse peritoneal macrophages as a function of age. Our results established the following observations: (a) resident macrophages from senescent mice expressed higher basal TGase activity than those from young mice; (b) Maximal TGase activity on day 3 of thioglycollate injection was lower by 24% in inflammatory macrophages from senescent as compared to young animals; (c) in contrast, as the inflammatory response abated (days 4-6), the incremental decrease in TGase activity in old was lower than in young animals; (d) in vitro activation of resident macrophages by retinol and mouse serum was more effective in inducing TGase activity from outbred CD-1 young mice than from inbred C57BL/6J young mice (age differences were also more prominent in the CD-1 mouse strain); and (e) Retinol and mouse serum effectively inhibited the production of superoxide in young mice, thereby demonstrating an inverse correlation between TGase activity and superoxide production. In old animals, however, the production of superoxide was not decreased, nor was TGase increased. Although, paradoxically, resident macrophages from senescent mice were a priori more activated than those from young ones, it is concluded that macrophages from young mice respond better than those from old ones to stimuli they encounter, either during inflammation or under physiological stimulation.
authors.affiliation: Department of Morphological Sciences, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Lavie
authors.forename: O
authors.initials: O
authors.lastname: Weinreb
mesh_terms: D000375:Aging / Q000097:blood*; D000818:Animals; D002478:Cells, Cultured; D004790:Enzyme Induction; D005260:Female; D007249:Inflammation / Q000201:enzymology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D016897:Respiratory Burst; D013268:Stimulation, Chemical; D013312:Stress, Physiological / Q000097:blood / Q000201:enzymology*; D013481:Superoxides / Q000378:metabolism; D011503:Transglutaminases / Q000096:biosynthesis / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology*; D015054:Zymosan
chemical_list: D013481:Superoxides; D014801:Vitamin A; D015054:Zymosan; D011503:Transglutaminases