Structural studies of the O-specific chain of Hafnia alvei strain 32 lipopolysaccharide.
The structure of the O-specific side chain of the Hafnia alvei strain 32 lipopolysaccharide has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradations, NMR spectroscopy, MALDI-TOF and FAB mass spectrometry in combination with collision-induced decomposition MS/MS were the principal methods used. It is concluded that the polysaccharide is composed of pentasaccharide repeating units having the following structure which is partially O-acetylated in the 2- (20%) and 3- (50%) position of the-->4)-alpha-D-GalpA-(1-->residue. [sequence :see text] A MALDI-TOF mass spectrum of the O-specific chains indicated that they consisted of up to 16 repeating units.
Jachymek
W
W
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wroclaw, Poland.
Petersson
C
C
Helander
A
A
Kenne
L
L
Niedziela
T
T
Lugowski
C
C
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009005:Monosaccharides; D019081:O Antigens