Enterotoxin-producing strains of Bacillus thuringiensis isolated from food.
Strains of Bacillus thuringiensis were isolated from various food items (pasta, pitta bread and milk) and were found to belong to either H-serotype kurstaki or neoleonensis. The strains were bioassayed against Pieris brassicae and insecticidal activity of strains was found to correspond to the presence of the cry1A-gene. All strains, except one, were found to express cytotoxic effects on Vero cells as an indicator of enterotoxin activity. Further, the B. thuringiensis strains HD-1 (serotype kurstaki), NB-125 (serotype tenebrionis) and HD-567 (serotype israelensis ) which are used commercially for insect pest management, were also found to have cytotoxic effects on Vero cells.
Damgaard
P H
PH
Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Larsen
H D
HD
Hansen
B M
BM
Bresciani
J
J
Jørgensen
K
K
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D002522:Chlorocebus aethiops; D004731:Endotoxins / Q000235:genetics; D004768:Enterotoxins / Q000378:metabolism*; D005516:Food Microbiology; D006460:Hemolysin Proteins; D007915:Lepidoptera / Q000382:microbiology; D012703:Serotyping; D014709:Vero Cells / Q000382:microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis