title: Production of recombinant SERA proteins of Plasmodium falciparum in Escherichia coli by using synthetic genes.
abstract: We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.
authors.affiliation: Department of Molecular Protozoology, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Suzue
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: J
authors.initials: J
authors.lastname: Inselburg
authors.forename: K
authors.initials: K
authors.lastname: Tai
authors.forename: T
authors.initials: T
authors.lastname: Horii
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D017125:Genes, Protozoan / Q000276:immunology*; D005813:Genes, Synthetic / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology; D016778:Malaria, Falciparum / Q000517:prevention & control; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D011994:Recombinant Proteins; C057495:serine repeat antigen, Plasmodium