Effects of Fe(2+), Zn(2+), Cu(2+) and Se(4+) on the synthesis and catabolism of prostaglandins in rabbit gastric antral mucosa.
Effects of Fe(2+), Zn(2+), Cu(2+) and Se(4+) on the synthesis and catabolism of prostaglandins (PGs) in rabbit gastric antral mucosa were examined. Fe(2+) inhibited the cyclooxygenase activity in the microsomal fraction. Zn(2+) suppressed the endoperoxide E(2) isomerase activity in the microsomal fraction and the 15-hydroxy PG dehydrogenase (PGDH) activity in the cytosolic fraction. Cu(2+) stimulated the cyclooxygenase activity, inhibited the PGDH activity and possibly induced non-enzymatic reduction of PGG(2) or PGH(2) to PGF(2 alpha) Se(4+) possibly induced the non-enzymatic reduction of PGG(2) or PGH(2) to PGF(2 alpha), as well as Cu(2+). These results suggest that Fe(2+), Zn(2+), Cu(2+) and Se(4+) can be modulators of the gastric antral mucosal PG levels by affecting the PG synthesizing enzymes, PG catabolizing enzyme and/or non-enzymatic reduction of PGG or PGH to PGF.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Kawai, Matsubara, Osaka, Japan.
Fujimoto
Y
Y
Miyata
Y
Y
Ohno
M
M
Nishida
H
H
Fujita
T
T
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019746:Intramolecular Oxidoreductases; D007501:Iron / Q000494:pharmacology; D007535:Isomerases / Q000037:antagonists & inhibitors; D019216:Metals, Heavy / Q000494:pharmacology*; D044262:Prostaglandin H2; D000071876:Prostaglandin-E Synthases; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D011462:Prostaglandins G / Q000378:metabolism; D011463:Prostaglandins H / Q000378:metabolism; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D019216:Metals, Heavy; D011453:Prostaglandins; D011462:Prostaglandins G; D011463:Prostaglandins H; D016718:Arachidonic Acid; D044262:Prostaglandin H2; C038291:prostaglandin G2; D003300:Copper; D015237:Dinoprost; D007501:Iron; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D011451:Prostaglandin-Endoperoxide Synthases; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D012643:Selenium; D015032:Zinc; D015232:Dinoprostone