A simple procedure for large-scale purification of 9-cis beta-carotene from Dunaliella bardawil.
For experiments designed to obtain reliable data on the metabolic pathway and biological function of 9-cis beta-carotene, sufficient quantity of this substance in pure form is needed. For this purpose, we decided to purify 9-cis beta-carotene from the dry powder of the alga Dunaliella bardawil. By use of both silica gel and ODS open column chromatography and high-performance liquid chromatography (HPLC) on an ODS column and by treatment of the sample with ethanol, we could obtain this carotenoid as fine needle-shaped orange crystals. They were found to be highly pure as judged by analytical HPLC, absorption spectrum, and nuclear magnetic resonance analysis.
Suzuki
T
T
Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Japan.
Nakashima
M
M
Ohishi
N
N
Yagi
K
K
D000460:Chlorophyta / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D019207:beta Carotene / Q000737:chemistry / Q000302:isolation & purification*
D019207:beta Carotene