Ontogeny of immunoreactive steroidogenic proteins (adrenodoxin and cytochrome p-450(21)) in the interrenals of the teleost Lates calcarifer.
Antisera against bovine adrenodoxin and cytochrome P-450(21) (steroid 21-hydroxylase) cross-reacted with the interrenal cells of the adult Asian seabass (Lates calcarifer); the cells were arranged as cords, two cells thick, in the headkidney. During larval development, cells immunoreactive for adrenodoxin were first observed 1 day posthatching (dph); immunoreactivity for cytochrome P-450(21) was first detected at 1.5 dph. Initially, the interrenal cells occurred as a mass in each headkidney, which only became identifiable histologically at 5 dph. The number of interrenal cells increased with age, becoming associated with the cardinal veins at 14 dph. The present study thus indicates that the posthatching rise in cortisol may originate from the nascent interrenal tissue.
Sampath-Kumar
R
R
Department of Zoology, National University of Singapore.
Munro
A D
AD
Lam
T J
TJ
D000325:Adrenodoxin / Q000032:analysis*; D000818:Animals; D001492:Bass / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D007401:Interrenal Gland / Q000201:enzymology / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000254:growth & development; D013255:Steroid 21-Hydroxylase / Q000032:analysis*
D000325:Adrenodoxin; D013255:Steroid 21-Hydroxylase