DNA sequence analysis by matrix-assisted laser desorption ionization MS.
The major advantage of this procedure is that, as detailed in Figure 5, the entire sequence of an oligonucleotide is determined in less than 1 h using inexpensive reagents. DE MS, because of its significantly higher resolving power, permits analysis of oligonucleotides as long as 50 bases, about twice as long as that which is possible without DE. Phosphorothioates can be sequenced after oxidation to the phosphodiester state. We have also found that the enzymes will digest through a number of modifying and protecting groups on DNA and RNA, including 2'-amino- and 2'-alkyl-substituted hydroxy groups. The resultant spectra can confirm not only the presence, but also the position, of modifying groups on both DNA and RNA molecules.
Haff
L A
LA
PerSeptive Biosystems, Framingham, MA 01701, USA.
Smirnov
I P
IP
D001331:Automation; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009838:Oligodeoxyribonucleotides; D004247:DNA