Specific guanylation of Lupinus luteus 5S rRNA at its 3' end in HeLa cell extract.
We have found that Lupinus luteus 5S ribosomal RNA is post-transcriptionally modified at its 3' end by addition of pG catalysed by HeLa cells extract. This extra nucleotide is not encoded within Lupinus luteus 5S rRNA gene. A similar reaction has been previously observed for eukaryotic histidine tRNAs. However an additional guanosine residue was ligated post-transcriptionally at the 5' end of that tRNA.
Wyszko
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Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznañ, Poland.
Szweykowska-Kulinska
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Barciszewska
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D001483:Base Sequence; D006151:Guanosine / Q000235:genetics*; D006160:Guanosine Triphosphate / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012639:Seeds / Q000235:genetics
D012341:RNA, Ribosomal, 5S; D006151:Guanosine; D006160:Guanosine Triphosphate