Detection of Marek's disease virus serotype 1 (MDV1) glycoprotein D in MDV1-infected chick embryo fibroblasts.
Chick embryo fibroblasts (CEFs) infected with three strains of Marek's disease virus serotype 1 (MDV1), GA, Md5 and JM, were subjected to indirect immunofluorescence assay with monoclonal antibodies (MAbs) against MDV1 homolog of glycoprotein D (MDV1 gD) of herpes simplex virus. By the MAbs, a number of MDV1 gD-positive cells were detected in CEFs infected with GA, whereas only a few and no positive cells were detected in CEFs infected with Md5 and JM, respectively. The MDV1 gD in GA-infected CEFs was recognized as the band of 64 kDa in immunoblot analysis using one of the MAbs. This is the first report that the MDV1 gD was detected in MDV1-infected cell cultures.
Ono
M
M
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Jang
H K
HK
Maeda
K
K
Kawaguchi
Y
Y
Tohya
Y
Y
Niikura
M
M
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002642:Chick Embryo; D005347:Fibroblasts; D019084:Fluorescent Antibody Technique, Indirect; D008381:Herpesvirus 2, Gallid / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012703:Serotyping; D018411:Spodoptera; D014162:Transfection; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C102727:glycoprotein D, Marek's disease virus