title: Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis.
abstract: Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.
authors.affiliation: School of Dentistry, Department of Pathology, São José dos Campos, UNESP, São Paulo, Brazil.
authors.forename: C S
authors.initials: CS
authors.lastname: Unterkircher
authors.forename: S C
authors.initials: SC
authors.lastname: Yazaki
authors.forename: M T
authors.initials: MT
authors.lastname: Shimizu
authors.forename: A O
authors.initials: AO
authors.lastname: Jorge
authors.forename: Z P
authors.initials: ZP
authors.lastname: Camargo
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000302:isolation & purification; D000946:Antigens, Fungal / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008875:Middle Aged; D010229:Paracoccidioidomycosis / Q000097:blood / Q000276:immunology*; D012016:Reference Values
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D007075:Immunoglobulin M