title: Effects of transient prepubertal 6-N-propyl-2-thiouracil treatment on testis development and function in the domestic fowl.
abstract: It has been well established that thyroid hormones play an important role in regulating the onset of puberty and reproductive function in birds. In mammals it has been shown that transient hypothyroidism induced with the reversible goitrogen 6-N-propyl-2-thiouracil (PTU) can result in tremendous increases in testis size and sperm production and that the timing of hypothyroidism must correspond to the period of Sertoli cell proliferation. As the period of Sertoli cell proliferation is not precisely known in the fowl, an experiment was conducted to determine whether chicken testes have a similar window of sensitivity to PTU treatment. Broiler breeder male chicks (Peterson) were placed in floor pens at one day of age and reared according to the breeder's management guide for the entire 28-wk duration (controls) or up to the point of dietary treatment with PTU (0.1% w:w) for 6 wk that began at 2-wk intervals (2-8, 4-10, 6-12, 8-14, and 10-16 wk of age); after treatment, birds were returned to feed restriction and photostimulated at 20 wk of age. Birds were bled and killed, and testes were collected at 4-wk intervals. At 28 wk, one testis was fixed for histological examination and one was immediately placed in liquid N2 for sperm counts. Treatment with PTU from 6 to 12 wk of age resulted in a 96% increase in mean testis weight at 28 wk of age (treated 39.3 +/- 4.1 g per testis vs. control 20.0 +/- 1.6 g per testis). These testes exhibited normal morphology and increased relative sperm production. Treatment with PTU from either 8 to 14 or 10 to 16 wk of age resulted in approximately a 35% increase in testis mass at 28 wk of age relative to the control value (27.2 +/- 2.0 g and 27.7 +/- 3.6 g vs. 20.0 +/- 1.6 g per testis, respectively). However, both of these groups clearly demonstrated precocious puberty and abnormal spermatogenesis. These results suggest that appropriately timed PTU treatment may result in permanent increases in testis size and sperm production in the domestic fowl.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Kirby
authors.forename: M V
authors.initials: MV
authors.lastname: Mankar
authors.forename: D
authors.initials: D
authors.lastname: Hardesty
authors.forename: D L
authors.initials: DL
authors.lastname: Kreider
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology*; D002645:Chickens; D015331:Cohort Studies; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D012671:Seminiferous Tubules / Q000187:drug effects / Q000502:physiology; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D013739:Testosterone / Q000097:blood / Q000378:metabolism; D013889:Thiouracil / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013956:Antithyroid Agents; D013739:Testosterone; D013889:Thiouracil