title: Serum trypsinogen-2 in the prediction of outcome in acute necrotizing pancreatitis.
abstract: BACKGROUND
  The accuracy of serum trypsinogen-2 in predicting the severity of acute necrotizing pancreatitis (ANP) was prospectively evaluated in 52 consecutive patients.
  
  
  METHODS
  A new sensitive immunofluorometric assay was used for serum trypsinogen-2,
  
  
  RESULTS
  Mean values during the first 24 h were 42.1 micrograms/l in control patients, 1435 micrograms/l in uncomplicated cases, and 4090 micrograms/l in complicated or fatal cases. There was a significant difference in serum trypsinogen-2 values between patients with uncomplicated and complicated disease (p = 0.002) already on admission. When a cutoff level of 1000 micrograms/l was used, patients with uncomplicated ANP were differentiated from patients with complicated ANP with a sensitivity of 91% and with a specificity of 71%.
  
  
  CONCLUSIONS
  The immunofluorometric assay of serum trypsinogen-2 is a sensitive and specific method for prediction of the severity of the disease in necrotizing pancreatitis.
authors.affiliation: Dept. II of Surgery, Helsinki University Central Hospital, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Sainio
authors.forename: P
authors.initials: P
authors.lastname: Puolakkainen
authors.forename: E
authors.initials: E
authors.lastname: Kemppainen
authors.forename: J
authors.initials: J
authors.lastname: Hedström
authors.forename: R
authors.initials: R
authors.lastname: Haapiainen
authors.forename: L
authors.initials: L
authors.lastname: Kivisaari
authors.forename: U H
authors.initials: UH
authors.lastname: Stenman
authors.forename: T
authors.initials: T
authors.lastname: Schröder
authors.forename: E
authors.initials: E
authors.lastname: Kivilaakso
mesh_terms: D000328:Adult; D000368:Aged; D000681:Amylases / Q000097:blood / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002097:C-Reactive Protein / Q000032:analysis; D005260:Female; D015200:Fluoroimmunoassay; D006801:Humans; D008297:Male; D008875:Middle Aged; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood* / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed; D014357:Trypsin; D014362:Trypsinogen / Q000097:blood* / Q000652:urine
chemical_list: D015415:Biomarkers; C064831:PRSS2 protein, human; D014362:Trypsinogen; D002097:C-Reactive Protein; D000681:Amylases; D014357:Trypsin