The proliferation of human T lymphocytes stimulated by Helicobacter pylori antigens.
Fractionated mononuclear cells (MNCs) were obtained from peripheral blood of healthy human volunteers, seronegative for H. pylori antibodies. The MNCs were stimulated in culture with whole live or heat-killed H. pylori cells or with bacterial cell surface (SA) or cytoplasmic (CA) antigens. There was a marked proliferative response of T cells in cultures stimulated with 10(5) cells/well of live H. pylori, 5 micrograms/well of CA or 5-20 micrograms/well of SA. However, no proliferation was observed in MNC cultures containing higher "doses" of live H. pylori organisms (10(7)/well) or CA (20 micrograms/well). Moreover, higher "doses" of the bacteria or CA entirely inhibited the response of T cells to PHA.
Chmiela
M
M
Department of Medical Microbiology, Lund University, Sweden.
Paziak-Domanska
B
B
Ljungh
A
A
Wadström
T
T
Rudnicka
W
W
D018829:Adhesins, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000494:pharmacology*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000276:immunology; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D013601:T-Lymphocytes / Q000276:immunology*
D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial