title: Quantitative evaluation of passive cutaneous anaphylaxis (PCA) using a hand-held spectrophotometer.
abstract: A hand-held spectrophotometer was used for the quantitative evaluation of PCA caused by anti-TNP-IgE and TNP-BSA. A good relationship existed between the dilution of sera and the value of delta E*ab (a numerical expression of color) obtained by a hand-held spectrophotometer. In addition, the value of delta E*ab and the amount of Evans blue measured by the conventional colorimetric method correlated well. Because the method using a hand-held spectrophotometer provides a simple and objective analysis, it appears that the method is suitable as a substitute for the conventional method, which is time-consuming and requires killing animals cruelly.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: R
authors.initials: R
authors.lastname: Teshima
authors.forename: R
authors.initials: R
authors.lastname: Akasaka
authors.forename: K
authors.initials: K
authors.lastname: Fujimori
authors.forename: Y
authors.initials: Y
authors.lastname: Goda
authors.forename: J
authors.initials: J
authors.lastname: Sawada
authors.forename: M
authors.initials: M
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry