title: Sub-typing of animal and human Campylobacter spp. using RAPD.
abstract: Based on a 10-mer primer (5'-CCTGTTAGCC-3'), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. The method was also successfully applied to typing Campylobacter jejuni from a similar range of sources.
authors.affiliation: Food Science Department, Department of Agriculture for Northern Ireland, Belfast, UK. R.Madden@QUB.AC.UK
authors.forename: R H
authors.initials: RH
authors.lastname: Madden
authors.forename: L
authors.initials: L
authors.lastname: Moran
authors.forename: P
authors.initials: P
authors.lastname: Scates
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000145:classification* / Q000235:genetics; D002169:Campylobacter Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013552:Swine / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial