Lectin-histochemical study of O-linked glycoconjugates in dysplastic retina of Norrie disease.
The carbohydrate chains of O-linked glycoconjugates in dysplastic retina from a Japanese female infant with Norrie disease were examined by lectin histochemistry. The avidin-biotinylated peroxidase method was used. The retina was highly dysplastic and composed of undifferentiated embryonic tissues containing a number of rosettes of varying sizes. The lumina of the rosettes were stained by peanut agglutinin, which recognizes the Gal beta 1,3GalNAc sequence of O-linked glycans. However, the lumina were not labeled by wheat germ agglutinin, which reacts with sialic acid and/or N-acetylglucosamine. These observations suggest that the O-linked glycoconjugates in the lumina of rosettes were not sialylated in the present case. Their lack of terminal sialic acids may be related to the rosette formation.
Uehara
F
F
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Sameshima
M
M
Yanagita
T
T
Iwakiri
N
N
Ohba
N
N
D000117:Acetylglucosamine / Q000378:metabolism; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D005124:Eye Abnormalities / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008607:Intellectual Disability / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D037102:Lectins; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D019887:Peanut Agglutinin; D011134:Polysaccharides / Q000378:metabolism; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D015792:Retinal Dysplasia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014909:Wheat Germ Agglutinins
D015295:Antigens, Tumor-Associated, Carbohydrate; D006001:Glycoconjugates; D037102:Lectins; D019887:Peanut Agglutinin; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins; C016467:Thomsen-Friedenreich antigen; D019158:N-Acetylneuraminic Acid; D000117:Acetylglucosamine