Measurement of cell adhesion and migration using phosphor-screen autoradiography.
This study demonstrates the use of phosphor-screen autoradiography as a means of measuring cell adhesion and cell expansion on polymer surfaces. The method has particular merit in cases where a specific substrate may be opaque or biochemically incompatible with colorimetric assay methodologies. With the phosphor-screen autoradiography method, there was a linear relationship between cell number and quantitated radioactivity. The technique has also been validated by comparison with a colorimetric assay of adhesion conducted for attachment to conventional culture substrata. The data supported the view that the use of phosphor-screen autoradiography was a valid method for detecting cell attachment, and it gave equivalent results to the colorimetric assay. Furthermore, a comparison between phosphor-screen autoradiography and a dye-staining method showed that this technique can be used as a means of quantifying cellular expansion over surfaces.
Dalton
B A
BA
CSIRO Division of Biomolecular Engineering, North Ryde, NSW, Australia. dalton@pelican.dbe.csiro.au
Dziegielewski
M
M
Johnson
G
G
Underwood
P A
PA
Steele
J G
JG
D001345:Autoradiography / Q000295:instrumentation* / Q000379:methods; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002465:Cell Movement; D003124:Colorimetry; D004396:Coloring Agents; D007091:Image Processing, Computer-Assisted; D008163:Luminescent Measurements; D008751:Methylene Blue; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013499:Surface Properties
D001672:Biocompatible Materials; D004396:Coloring Agents; D008751:Methylene Blue