title: RT-PCR analysis of MMP-9 expression in human articular cartilage chondrocytes and synovial fluid cells.
abstract: This report presents a method for analyzing matrix metalloproteinase-9 (92 kD gelatinase) MMP-9 mRNA expression using reverse transcription polymerase chain reaction (RT-PCR). With this method, chondrocytes isolated from small samples of osteoarthritic cartilage showed significantly elevated signal for MMP-9 mRNA compared to normal cartilage. In addition, cells of synovial fluid samples aspirated from osteoarthritic joints also exhibited MMP-9 expression using this technique. RT-PCR proved to be a sensitive method for assessing MMP-9 regulation in osteoarthritic and normal cartilage, and may provide a useful index of arthritic cartilage and synovial fluid cell metabolism when limited tissue is available.
authors.affiliation: Orthopaedic Research Laboratory, Stanford University School of Medicine, California 94305-5341, USA.
authors.forename: K
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: W J
authors.initials: WJ
authors.lastname: Maloney
authors.forename: T
authors.initials: T
authors.lastname: Vu
authors.forename: A R
authors.initials: AR
authors.lastname: Hoffman
authors.forename: D J
authors.initials: DJ
authors.lastname: Schurman
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
mesh_terms: D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9; D010003:Osteoarthritis / Q000201:enzymology / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013582:Synovial Fluid / Q000166:cytology / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D017364:Collagenases; D020780:Matrix Metalloproteinase 9