Production of recombinant SERA proteins of Plasmodium falciparum in Escherichia coli by using synthetic genes.
We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.
Sugiyama
T
T
Department of Molecular Protozoology, Osaka University, Japan.
Suzue
K
K
Okamoto
M
M
Inselburg
J
J
Tai
K
K
Horii
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D017125:Genes, Protozoan / Q000276:immunology*; D005813:Genes, Synthetic / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology; D016778:Malaria, Falciparum / Q000517:prevention & control; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D011994:Recombinant Proteins; C057495:serine repeat antigen, Plasmodium