Effect of feeding raw soybeans on polyamine metabolism in chicks and the therapeutic effect of exogenous putrescine.
The inclusion of isolated soy protein in milk replacer diets for calves and neonatal pigs inhibits development of intestinal mucosal cells. Simultaneous administration of putrescine partially overcomes this effect. We therefore conducted experiments to determine the potential for dietary putrescine to overcome the toxicity of raw soybeans in chicks. In the first experiment, week-old chicks were fed either an isolated soy protein-based control diet or an isoenergetic and isonitrogenous diet containing 52% raw, ground soybeans for 14 d. The feeding of raw soybeans depressed (P < .001) growth and feed consumption, caused enlargement (P < .001) of the duodenum and pancreas, depressed (P < .001) activities of polyamine synthetic enzymes in the duodenum, and reduced (P < .01) duodenal tissue concentrations of putrescine. In the second experiment, the diet containing raw soybeans was fed with and without .2, .3, and .4% supplemental putrescine. The feeding of supplemental putrescine largely overcame the inhibition of growth due to the feeding of raw soybeans and increased intestinal putrescine concentrations. Putrescine supplementation had no effect, however, on pancreatic and intestinal enlargement in birds fed raw soybeans and tended to depress the activity of polyamine synthetic enzymes. The beneficial effects of putrescine supplementation were confirmed in the third experiment when up to 1.0% supplemental putrescine was fed. We conclude that the toxicity of raw soybeans to chicks can be overcome by feeding putrescine. These effects are likely due to improved nutrient uptake by overcoming the adverse effects of lectins in the intestinal tract and are not likely due to alleviation of the pancreatic enlargement caused by protease inhibitors.
Mogridge
J L
JL
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada.
Smith
T K
TK
Sousadias
M G
MG
D012437:Adenosylmethionine Decarboxylase / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D004032:Diet / Q000662:veterinary; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D004435:Eating / Q000502:physiology; D005527:Food, Fortified; D007082:Ileum / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D008297:Male; D009955:Ornithine Decarboxylase / Q000032:analysis; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000008:administration & dosage / Q000494:pharmacology*; D013025:Glycine max / Q000592:standards*
D011073:Polyamines; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D011700:Putrescine