title: Serum levels of endothelial injury markers creatine kinase-BB and soluble thrombomodulin during human liver transplantation.
abstract: Endothelial damage within the sinusoids of the liver probably plays a key role in primary liver dysfunction following transplantation. The aim of this work was to study the serum levels of two potential markers of endothelial damage, creatine kinase-BB and soluble thrombomodulin, during human graft revascularization. Thirteen human liver grafts were preserved in UW solution (mean time: 13.8 h). Creatine kinase-BB and transaminase activities and soluble thrombomodulin levels were measured: 1) in effluent and 2) in serum samples sequentially collected before revascularization, then during the first 120 min of revascularization and first post-operative week. No correlation was observed between serum values (peak) and effluent values. In serum, pre-operative creatine kinase-BB activities were correlated with soluble thrombomodulin levels (p = 0.01). Both increased significantly during the first minutes of the revascularization, then decreased markedly. In contrast, AST activity was maximal at day 1. This detectable and early release of creatine kinase-BB and soluble thrombomodulin in blood is in keeping with the early occurence of endothelial damage. Together with previous data, these findings suggest that serum determination of these two markers may be a useful tool in the assessment of endothelial injury in liver transplantation.
authors.affiliation: Unité d'Hépatologie, Hôpital Saint Antoine, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Chazouilleres
authors.forename: M
authors.initials: M
authors.lastname: Vaubourdolle
authors.forename: A
authors.initials: A
authors.lastname: Robert
authors.forename: V
authors.initials: V
authors.lastname: Fourel
authors.forename: P
authors.initials: P
authors.lastname: Balladur
authors.forename: A
authors.initials: A
authors.lastname: Laribi
authors.forename: J
authors.initials: J
authors.lastname: Giboudeau
authors.forename: R
authors.initials: R
authors.lastname: Parc
authors.forename: R
authors.initials: R
authors.lastname: Poupon
authors.forename: L
authors.initials: L
authors.lastname: Hannoun
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D003402:Creatine Kinase / Q000097:blood* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004730:Endothelium, Vascular / Q000737:chemistry / Q000293:injuries*; D006801:Humans; D007527:Isoenzymes; D016031:Liver Transplantation; D018709:Statistics, Nonparametric; D018180:Thrombomodulin / Q000097:blood* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D018180:Thrombomodulin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase