Molecular cloning and expression of a mouse alpha-1,3 fucosyltransferase gene that shows homology with the human alpha-1,3 fucosyltransferase IV gene.
The polymerase chain reaction was used to amplify and clone a fragment of a novel fucosyltransferase cDNA from mouse embryonal carcinoma F9 cells. Southern blot analysis of mouse genomic DNA with the cloned cDNA fragment revealed that there is a single copy of the fucosyltransferase gene per haploid genome. A 5 kb EcoRI restriction fragment of the genomic DNA hybridized with the cDNA has been cloned and a 1,697 bp fragment containing the sequence of the cDNA was sequenced. The nucleotide sequence and the deduced amino acid sequence of the clone showed a highest degree of homology with those of human alpha-1,3 fucosyltransferase IV, i.e. 77.1 and 75.5% identity, respectively. Consistent with the sequence similarity, the transfection of a 1,544 bp fragment under the control of the cytomegarovirus enhancer and beta actin promoter into COS-1 cells confers alpha-1,3 fucosyltransferase activity on the cells, resulting in cell surface expression of SSEA-1.
Ozawa
M
M
Department of Biochemistry, Kagoshima University.
Muramatsu
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005647:Fucosyltransferases / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase