beta-agonists in animal feed. III: Optimization of the clean-up and the end-determination step.
This study represents the second part of an interlaboratory study intended to develop an official modular Community confirmatory method for the detection of beta-agonists in animal feed. Homogeneous pools of primary extracts were prepared by means of an extraction module based on the conclusions of a previous part of this work. The primary extracts were further processed by four laboratories each using a different clean-up scheme. The final extracts thus obtained were cross-distributed between the same laboratories and measured either by GCMS or HPLC. Two laboratories (B and D) applied separate clean-up schemes for clenbuterol and salbutamol. All clean-up schemes for clenbuterol were found to be compatible with all end-determination steps. In contrast, for salbutamol clean-up method D was found not to be compatible with the end-determination steps applied by laboratories B and C. The results of this study have clearly demonstrated that the clean-up methods for both clenbuterol and salbutamol applied by laboratory B yielded superior recoveries with an acceptable standard deviation. Therefore, in conclusion to this study, the participating laboratories recommend the clean-up schemes applied by laboratory B to serve as part of the official Community confirmatory method.
Leyssens
L
L
Dr L. Willems-Instituut, Diepenbeek, Belgium.
Courtheyn
D
D
Schilt
R
R
Berthoz
Y
Y
Moermans
R
R
Boenke
A
A
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000420:Albuterol / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002976:Clenbuterol / Q000032:analysis*; D005525:Food-Processing Industry / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D012015:Reference Standards
D000318:Adrenergic beta-Agonists; D000420:Albuterol; D002976:Clenbuterol