Effect of aluminium hydroxide administration on normal mice: tissue distribution and ultrastructural localization of aluminium in liver.
In order to assess the risk of parenteral aluminium (Al) exposure, we evaluated the effects of intraperitoneal administration of aluminium hydroxide, a compound widely used in medicine. Mice (strain Pzh:SFIS) received intraperitoneally, every two weeks 1 mg Al or 0.1 mg Al for five days a week. Controls received injections of saline. Al concentrations in liver, bone and brain were evaluated by electrothermal atomic absorption spectrometry after exposure to 2 mg, 4 mg, and 6 mg Al. The concentration was the highest in liver and occurred after exposure to only 2 mg Al (265.1 +/- 27.7 mg/kg, 233.5 +/- 28.0 mg/kg). Generally further accumulation was not dose- and treatment-dependent. The only exception was a significant Al increase in the liver after exposure to 6 mg Al, injected 0.1 mg Al five days/week. Development of resorption granulomas was observed in the liver, Al being revealed by Morin fluorescence in constituent macrophages and giant cells. By electron probe X-ray microanalysis, Al was identified predominantly in lysosomes of macrophages and Kupffer cells. In tibia of mice, a dose-dependent Al accumulation was observed. The highest level of Al concentration after the 6 mg treatment was 23.5 +/- 3.82 mg/kg and 25.06 +/- 2.3 mg/kg. The Al concentration in the brain of mice had not changed significantly during Al treatment.
Fiejka
M
M
Department of Serum and Vaccine Control, National Institute of Hygiene, Warsaw, Poland.
Fiejka
E
E
Døugaszek
M
M
D000535:Aluminum / Q000032:analysis*; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology*; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure; D001923:Brain Chemistry / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008099:Liver / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008815:Mice, Inbred Strains
D000536:Aluminum Hydroxide; D000535:Aluminum