title: Cautionary note on the use of dUMP-containing PCR primers with Pfu and VentR DNA polymerases.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284, USA. sakaguchi@uthscsa.edu
authors.forename: A Y
authors.initials: AY
authors.lastname: Sakaguchi
authors.forename: M
authors.initials: M
authors.lastname: Sedlak
authors.forename: J M
authors.initials: JM
authors.lastname: Harris
authors.forename: M F
authors.initials: MF
authors.lastname: Sarosdy
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular / Q000379:methods*; D045647:DNA Glycosylases; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D013379:Substrate Specificity; D051981:Uracil-DNA Glycosidase
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D003856:Deoxyuracil Nucleotides; D005656:Fungal Proteins; D009687:Nuclear Proteins; C007267:2'-deoxyuridylic acid; C072146:Pfu DNA polymerase; C070331:Tli polymerase; D004259:DNA-Directed DNA Polymerase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase