Niflumic acid-induced increase in potassium currents in frog motor nerve terminals: effects on transmitter release.
The actions of the nonsteroidal antiinflammatory drug niflumic acid were studied on frog neuromuscular preparations by conventional electrophysiological techniques. Niflumic acid reduced the amplitude and increased the latency of endplate potentials in a concentration-dependent manner. Neuromuscular junctions pretreated with niflumic acid (0.05-0.5 mM) showed much less depression than control when they were stimulated with trains of impulses. Inhibition of acetylcholine release was reverted by raising the extracellular Ca(2+) concentration but not by simply washing out the preparations with niflumic acid-free solutions. Pretreatment with indomethacin (0.1 mM), another nonsteroidal antiinflammatory drug, did not affect the niflumic acid-induced inhibition of evoked responses. Niflumic acid (0.1 mM) did not change the amplitude of miniature endplate potentials and had a dual action on the frequency of miniatures: it decreased their frequency at 0.1 mM whereas it produced an enormous increase in the rate of spontaneous discharge at 0.5 mM. Niflumic acid (0.1 - 1 mM) reversibly increased the amplitude and affected the kinetics of presynaptic voltage-activated K+ current and Ca(2+)-activated K(+) current in a concentration-dependent manner. Niflumic acid (0.1 - 1 mM) irreversibly decreased the amplitude and reversibly affected the kinetics of the nodal Na(+) current. Indomethacin (0.1 mM) had no effect on presynaptic currents. In conclusion, niflumic acid reduces acetylcholine release by increasing presynaptic K+ currents. This may shorten the depolarizing phase of the presynaptic action potential and may reduce the entry of Ca(2+) with each impulse.
Miralles
F
F
Departament de Biologia Cellular i Anatomia Patològica, Hospital de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
Marsal
J
J
Peres
J
J
Solsona
C
C
D000818:Animals; D004305:Dose-Response Relationship, Drug; D008564:Membrane Potentials / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects*; D009412:Nerve Fibers / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009544:Niflumic Acid / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects*; D011893:Rana esculenta
D018377:Neurotransmitter Agents; D015221:Potassium Channels; D009544:Niflumic Acid