The reduction of preprotachykinin mRNA in the methamphetamine-induced behavioral sensitization.
To study the role of the preprotachykinin A (PPT) mRNA in methamphetamine (MAP)-induced behavioral sensitization, rats were daily injected within MAP (3 or 6 mg/kg, i.p., once a day) or saline for 14 days. Progressive enhancement was observed in MAP-induced stereotyped behavior. After 7 or 14 days of discontinuation of MAP treatment, the rats were decapitated and the brains were prepared for Northern blot analysis using a 32P-labeled oligonucleotide probe. Northern blot analysis revealed that the level of PPT mRNA in the striatum of 3 or 6 mg/kg MAP-treated rats was significantly decreased, compared to that in the saline-treated controls. These findings indicate that PPT mRNA expression plays an important role in MAP-induced long-lasting behavioral sensitization and dopamine hyperactivity.
Fukamauchi
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Department of Molecular Medical Science, Tokyo Medical and Dental University, Japan.
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal / Q000187:drug effects*; D015152:Blotting, Northern; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004298:Dopamine / Q000502:physiology; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D015345:Oligonucleotide Probes; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D015320:Tachykinins / Q000096:biosynthesis*
D000199:Actins; D000697:Central Nervous System Stimulants; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; D008694:Methamphetamine; D004298:Dopamine