In vitro model of parathyroid hormone-related protein secretion from mammary cells isolated from lactating cows.
Parathyroid hormone-related protein (PTHrP) is produced by the lactating mammary gland and is present in milk in a biologically active form. The goal of this investigation was to determine if cells cultured from the lactating mammary glands of cows would secrete PTHrP in vitro. Mammary acini were isolated from lactating cows at 1-6 wk after calving, and fresh or cryopreserved mammary acini were cultured for 14 d on Type I collagen. Cultures on thick layers of collagen (2.5 mm) were detached and allowed to contract on Day 6. PTHrP production was measured by N-terminal radioimmunoassay and bioassay (increased cAMP levels in ROS 17/2.8 osteoblast-like cells). The mammary cells reached confluence at Day 6. PTHrP production was low at Day 2 (< 0.5 ng/ml) but increased to peak production (2-4 ng/ml) at approximately Day 6 and remained constant until Day 14. Immunoreactive and bioactive PTHrP levels in the culture medium correlated well. The cultures produced lactoferrin (2,000-2,300 ng/ml and alpha s1-casein (14-19 ng/ml). Prolactin stimulated PTHrP production approximately 50% on Days 6-14. PTHrP production was increased approximately 100% by treatment with epidermal growth factor (10 ng/ml) for 2 d. Morphologic evaluation of cultures on thick, contracted collagen at Day 14 revealed an inner layer of mammary epithelial cells overlying myoepithelial cells and an outer layer of collagen containing stromal cells. Immunohistochemistry demonstrated positive staining for PTHrP and cytokeratin in both mammary epithelial and myoepithelial cells and alpha-smooth muscle actin in myoepithelial cells. These data demonstrated that cryopreserved mammary tissue from lactating cows could be cultured in vitro and secreted PTHrP in a regulated manner. This in vitro model will be useful to investigate the function and regulation of PTHrP in the lactating mammary gland.
Okada
H
H
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University; Columbus, USA.
Schanbacher
F L
FL
McCauley
L K
LK
Weckmann
M T
MT
Capen
C C
CC
Rosol
T J
TJ
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D015925:Cryopreservation; D000242:Cyclic AMP / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D007774:Lactation / Q000502:physiology*; D007781:Lactoferrin / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010006:Osteoblasts; D010281:Parathyroid Hormone / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein; D011388:Prolactin / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
D002364:Caseins; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D004815:Epidermal Growth Factor; D011388:Prolactin; D000242:Cyclic AMP; D007781:Lactoferrin; D000262:Adenylyl Cyclases