Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole.
In order to develop a stain with increased sensitivity and selectivity for cadmium (Cd), we synthesized and characterized 2-(8-quinolylazo)-4,5-diphenylimidazole (QAI). This chelating agent was more than twice as sensitive for Cd than the best conventional staining agents, including benzothiazolylazo-beta-naphthol. Differentiation between Cd and zinc (Zn) was achieved by immersing tissue sections in TRIS(2-aminoethyl)amine before they were stained with QAI. This pretreatment made it possible to selectively stain for Cd by blocking Zn.
Sumi
Y
Y
Department of Chemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Itoh
M T
MT
Muraki
T
T
Suzuki
T
T
D000818:Animals; D001391:Azo Compounds; D002104:Cadmium / Q000032:analysis*; D002614:Chelating Agents; D005029:Ethylenediamines; D006651:Histocytochemistry / Q000379:methods*; D007093:Imidazoles; D008099:Liver / Q000737:chemistry*; D051381:Rats; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D015032:Zinc / Q000032:analysis
D001391:Azo Compounds; D002614:Chelating Agents; D005029:Ethylenediamines; D007093:Imidazoles; D002104:Cadmium; C099539:tris(2-aminoethyl)amine; C103928:2-(8-quinolylazo)-4,5-diphenylimidazole; D015032:Zinc