title: Transcription of c-mos protooncogene in the pig involves both tissue-specific promoters and alternative polyadenylation sites.
abstract: The function of the c-mos gene has been intensively studied, but its role in the mammal is still a subject for debate. For this reason, and because the gene is regulated posttranscriptionally, further study of the gene from other mammalian species is timely. The pig c-mos gene has been cloned, and the genomic sequence is presented here. The gene has no introns and shows close similarity to human and monkey genes, with striking sequence similarities in both the 5' and 3' flanking regions. The significance of this similarity in the context of gene regulation is discussed. c-mos expression was found to be restricted to gonadal tissues in the pig. The major start sites for transcription initiation in ovary and testis were identified by primer extension and found to be distinct, as in the mouse. Within the ovary, expression is confined to oocytes. Messenger RNA is synthesized in growing oocytes, and remains stable during oocyte maturation, but begins to be degraded in electrically stimulated eggs. Unexpectedly, RNase protection assays revealed that the 3' ends of transcripts in the pig ovary are heterogeneous, and this, together with the identification of three distinct cDNA clones, shows that multiple polyadenylation sites are used. The significance of these transcripts in terms of translational control is discussed.
authors.affiliation: Department of Development and Signalling, Babraham Institute, Cambridge, UK.
authors.forename: B
authors.initials: B
authors.lastname: Newman
authors.forename: Y
authors.initials: Y
authors.lastname: Dai
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000254:growth & development; D010053:Ovary; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics*; D012333:RNA, Messenger; D013552:Swine; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D017231:Proto-Oncogene Proteins c-mos