Screening of potential transport systems for methyl mercury uptake in rat erythrocytes at 5 degrees by use of inhibitors and substrates.
The current study was designed to screen the potential transport systems for methyl mercury (MeHg) uptake by isolated erythrocytes from rats at 5 degrees. Several inhibitors and substrates were used to test which potential transport system might be involved in MeHg uptake. Probenecid was used to test the organic anion transport system, valinomycin was used to test the effect of the membrane potential, D-glucose and cytochalasin B were used to test the facilitated diffusive D-glucose transport system and colchicine and vinblastine were used to test the microtubule system. The effects of Ca++, Mg++ and Na+ on MeHg uptake have been examined. Ouabain, ATP and glucose were used to test the active transport system, cysteine for the cysteine-facilitated transport system, glycine for system Gly, DL-methionine for system L, and MeHgCl and 4',4-diisothiocyano-2',2-stilbenedisulfonic acid (DIDS) for the Cl- ion transport system. The results showed that MeHg uptake might be involved in the following transport systems at 5 degrees: 1) organic anion transport system; 2) facilitated diffusive D-glucose transport system; 3) cysteine-facilitated transport system; 4) Cl- ion transport system. Moreover, the transport systems for MeHg uptake were sensitive to the membrane potential. Although the mechanisms of interaction of transport systems have not been fully clarified, evidence has been presented which support the existence of several simultaneous transport systems for MeHg uptake.
Wu
G
G
Biochemistry Section, National Institute for Minamata Disease, Kumamoto, Japan.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000097:blood / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002712:Chlorides / Q000097:blood; D003078:Colchicine / Q000378:metabolism / Q000494:pharmacology; D003080:Cold Temperature; D003571:Cytochalasin B / Q000097:blood / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000097:blood / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007476:Ionophores / Q000097:blood / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008767:Methylmercury Compounds / Q000097:blood* / Q000493:pharmacokinetics; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D010042:Ouabain / Q000097:blood / Q000494:pharmacology; D011339:Probenecid / Q000097:blood / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012076:Renal Agents / Q000097:blood / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D014634:Valinomycin / Q000097:blood / Q000494:pharmacology; D014747:Vinblastine / Q000378:metabolism / Q000494:pharmacology
D002712:Chlorides; D004791:Enzyme Inhibitors; D007476:Ionophores; D008767:Methylmercury Compounds; D009004:Monosaccharide Transport Proteins; D012076:Renal Agents; D014634:Valinomycin; D003571:Cytochalasin B; D010042:Ouabain; D014747:Vinblastine; D012964:Sodium; D008274:Magnesium; D005947:Glucose; D011339:Probenecid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D003078:Colchicine; D002118:Calcium