title: GABAA receptor-mediated responses in the ventromedial nucleus of the hypothalamus of female and male neonatal rats.
abstract: Perinatal exposure of the developing brain to gonadal steroids during a limited critical period induces permanent, organizational differences in neural structures between male and female animals. These differences are believed to underlie the manifestation of sexually dimorphic behaviors. Gamma-aminobutyric acid type A (GABAA) receptors expressed in the ventromedial nucleus (VMN) of the hypothalamus appear to be a key component underlying the expression of sexually dimorphic, and GABAA-mediated transmission within the VMN is critical for the expression of sexual behavior in female, but not male, rats. Here we report that analysis of VMN neurons from neonatal rats revealed significant sex-specific differences in GABAA receptor channel properties. Specifically, GABAA-mediated currents elicited by direct agonist application decayed more rapidly in VMN neurons from females than from males. Kinetic differences became more pronounced during the first 2 weeks of postnatal development. Analysis of small, spontaneous inhibitory postsynaptic currents recorded in intact slices indicated a trend towards slower responses in neurons from males than females, but the differences in decay kinetics were not significant. Sex-specific differences in GABAA receptor kinetics may arise from activation of receptors not receiving synaptic contacts in the slice preparation or may become apparent at intact synapses under conditions of increased activity and evoked release.
authors.affiliation: Department of Physiology, Dartmouth Medical School, Hanover, NH 03755 USA.
authors.forename: S T
authors.initials: ST
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Brennan
authors.forename: A S
authors.initials: AS
authors.lastname: Clark
authors.forename: L P
authors.initials: LP
authors.lastname: Henderson
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D004553:Electric Conductivity; D005260:Female; D008297:Male; D051381:Rats; D018079:Receptors, GABA / Q000502:physiology*; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D000255:Adenosine Triphosphate