title: K-ras and p53 mutations in hamster pancreatic ductal adenocarcinomas and cell lines.
abstract: K-ras and p53 gene alterations are frequently found in human pancreatic carcinomas and cell lines. The aim of this study was to analyze for the presence of K-ras and p53 gene mutations in hamster pancreatic tumors and cell lines. Mutations at the first coding exon of the K-ras gene and in exons V to VIII of the hamster p53 gene were analyzed in six cell lines (H2T, PC1, PC1.2, PC1.0, WD, and PD) and in N-nitroso-bis(2-oxopropyl)amine-induced pancreatic (n = 9) and extra-pancreatic (n = 4) tumors. K-ras mutations were present in seven of the nine pancreatic tumors and in all extra-pancreatic tumors. No p53 mutations were detected in the tumors. All cell lines analyzed contained K-ras mutations. Moreover, four of the six cell lines contained single amino acid substitutions in the p53 gene. Cell lines derived from nitrosamine-induced pancreatic tumors in the hamster contained K-ras and p53 alterations similar to those found in cell lines derived from human pancreatic carcinomas.
authors.affiliation: Laboratory of Gastrointestinal Investigation, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Erill
authors.forename: M
authors.initials: M
authors.lastname: Cuatrecasas
authors.forename: F J
authors.initials: FJ
authors.lastname: Sancho
authors.forename: A
authors.initials: A
authors.lastname: Farré
authors.forename: P M
authors.initials: PM
authors.lastname: Pour
authors.forename: F
authors.initials: F
authors.lastname: Lluís
authors.forename: G
authors.initials: G
authors.lastname: Capellá
mesh_terms: D000818:Animals; D002273:Carcinogens; D018270:Carcinoma, Ductal, Breast / Q000139:chemically induced / Q000235:genetics*; D006224:Cricetinae; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine