The uptake of Li+ into human 1321 N1 astrocytomas using 7Li NMR spectroscopy.
The uptake of Li+ ions into human 1321 N1 astrocytomas cultured on the surface of microcarrier beads was followed by 7Li NMR spectroscopy. The intracellular and extracellular 7Li resonances were separated by the use of dysprosium tripolyphosphate as a shift reagent. Excellent spectra were obtained from which the uptake of Li+ was found to be approximately ten times faster than that into human erythrocytes using the same technique and a steady-state intracellular Li+ concentration was observed within 60 min. The low intracellular Li+ concentration attained, relative to the extracellular concentration, indicates the presence of an efflux mechanism in astrocytomas that actively transports Li+ against its concentration gradient. The intracellular volume was estimated by quantitative 23Na NMR spectroscopy and the viability of the cells was confirmed by 31P NMR spectroscopy.
Bramham
J
J
Department of Biochemistry, University, Dundee, Scotland.
Carter
A N
AN
Riddell
F G
FG
D001254:Astrocytoma / Q000378:metabolism*; D001692:Biological Transport; D006801:Humans; D008094:Lithium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D014407:Tumor Cells, Cultured
D008094:Lithium