title: Effects of glycerol of beta-lactamase production during high cell density cultivation of recombinant Escherichia coli.
abstract: Recombinant Escherichia coli was cultured up to OD600 90 with glycerol fed by the DO-STAT method to produce recombinant beta-lactamase. Optimum feeding and induction conditions were determined. When glycerol was maintained at a high concentration (40-50 g/L) after isopropyl thiogalactoside (IPTG) induction at OD600 75, a 2-fold increase in the amount of soluble beta-lactamase was obtained. Similarly, a 3-fold increase was obtained by the addition of NaCl (0.4 M) at low glycerol concentration (10 g/L).
authors.affiliation: Department of Biological Engineering, Inha University, Inchon, Namgu, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kwon
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: E
authors.initials: E
authors.lastname: Kim
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins; D001618:beta-Lactamases; D005990:Glycerol