[Microtubules: functional polymorphisms of tubulin and associated proteins (structural and motor MAP's)].
In neuronal cells, microtubules are built from a very large number of alpha- and beta-tubulin variants. This diversity is due to the expression of a multigene family and to a combination of several original posttranslational modifications. Similarly, structural and motor microtubule-associated proteins, which regulate the assembly of microtubules, the modeling of their network and the mediation of their functions, are also very heterogeneous. As a consequence, mixing of these two protein polymorphisms leads to the formation of functionally-distinct microtubules. We have shown that polyglutamylation, the major posttranslational modification of neuronal tubulin, was used as a progressive regulator in the binding of structural and motor microtubule-associated proteins, in modulating gradually the conformation of the tubulin carboxy-terminal domain, playing thus a crucial role in microtubule dynamics.
Regnard
C
C
Biochimie Cellulaire, Collège de France, Paris, France.
Audebert
S
S
Boucher
D
D
Larcher
J C
JC
Eddé
B
B
Denoulet
P
P
D000818:Animals; D004347:Drug Interactions; D066298:In Vitro Techniques; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D008870:Microtubules / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011099:Polyglutamic Acid / Q000378:metabolism; D011110:Polymorphism, Genetic; D011499:Protein Processing, Post-Translational; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D014404:Tubulin; D011099:Polyglutamic Acid