Effects of mutating leucine to threonine in the M2 segment of alpha1 and beta1 subunits of GABAA alpha1beta1 receptors.
The conserved leucine residues at the 9' positions in the M2 segments of alpha1 (L264) and beta1 (L259) subunits of the human GABAA receptor were replaced with threonine. Normal or mutant alpha1 subunits were co-expressed with normal or mutant beta1 subunits in Sf9 cells using the baculovirus/Sf9 expression system. Cells in which one or both subunits were mutated had a higher "resting" chloride conductance than cells expressing wild-type alpha1beta1 receptors. This chloride conductance was blocked by 10 mM penicillin, a recognized blocker of GABAA channels, but not by bicuculline (100 microm) or picrotoxin (100 microm) which normally inhibit the chloride current activated by GABA: nor was it potentiated by pentobarbitone (100 microM). In cells expressing wild-type beta1 with mutated alpha1 subunits, an additional chloride current could be elicited by GABA but the rise time and decay were slower than for wild-type alpha1beta1 receptors. In cells expressing mutated beta1 subunits with wild-type or mutated alpha1 subunits (alphabeta(L9'T) and alpha(L9'T)beta(L9'T)), no response to GABA could be elicited: this was not due to an absence of GABAA receptors in the plasmalemma because the cells bound [3H]-muscimol. It was concluded that in GABAA channels containing the L9'T mutation in the beta1 subunit, GABA-binding does not cause opening of channels, and that the L9'T mutation in either or both subunits gives an open-channel state of the GABAA receptor in the absence of ligand.
Tierney
M L
ML
John Curtin School of Medical Research, Australian National University, Canberra, Australia.
Birnir
B
B
Pillai
N P
NP
Clements
J D
JD
Howitt
S M
SM
Cox
G B
GB
Gage
P W
PW
D016367:Baculoviridae; D002460:Cell Line; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D004594:Electrophysiology; D006801:Humans; D017136:Ion Transport; D007930:Leucine / Q000235:genetics; D017354:Point Mutation; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013912:Threonine / Q000235:genetics
D018118:Chloride Channels; D011963:Receptors, GABA-A; D013912:Threonine; D007930:Leucine