Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies.
p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the flow cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.
Landberg
G
G
Department of Pathology, University of Umeå, Sweden. Goran.Landberg@pathol.umu.se
Erlanson
M
M
Roos
G
G
Tan
E M
EM
Casiano
C A
CA
D000951:Antigens, Neoplasm / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology; D002455:Cell Division; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D019337:Hematologic Neoplasms / Q000276:immunology; D006801:Humans; D007399:Interphase; D008297:Male; D008840:Microfilament Proteins; D015530:Nuclear Matrix / Q000737:chemistry*; D016196:S Phase; D016707:Tissue Fixation; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D001324:Autoantigens; D014408:Biomarkers, Tumor; D002868:Chromosomal Proteins, Non-Histone; D004273:DNA, Neoplasm; D008840:Microfilament Proteins; C094772:centromere protein F