Epitope mapping of capsid proteins VP2 and VP3 of infectious bursal disease virus.
Twenty hybridoma cell lines producing monoclonal antibodies (MAbs) against serotype 1 infectious bursal disease virus (IBDV) of GBF-1 and the attenuated GBF-1E strains were produced. The MAbs recognized major structural proteins VP2 and VP3. MAb recognition sites were mapped using recombinant Escherichia coli clones which expressed N-terminal and (or) C-terminal truncated virus antigens, and competitive-binding assays. At least 3 conformation-dependent serotype 1 specific virus neutralizing antigenic sites and a linear antigenic site were defined on VP2 and VP3, respectively. Two of the conformational virus neutralizing antigenic sites were localized in the central area of VP2 consisting of 156 amino acid residues, and the linear epitope was localized in C-terminal 105 amino acid residues of VP3. Another conformational virus neutralizing antigenic site recognized with the virus neutralizing MAb GK-5 was not defined because GK-5 did not react with virus antigen expressed in recombinant E. coli. The conformational antigenic site was supposed to be composed of tertiary or quaternary protein structure, which may not be constructed in recombinant E. coli.
Yamaguchi
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Iwata
K
K
Kobayashi
M
M
Ogawa
M
M
Fukushi
H
H
Hirai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte; D015151:Immunoblotting; D007243:Infectious bursal disease virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015678:Viral Structural Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins