title: Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis.
abstract: OBJECTIVE
  To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen.
  
  
  METHODS
  The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a beta-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against beta-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm.
  
  
  RESULTS
  A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C trachomatis hsp60.
  
  
  CONCLUSIONS
  This assay will permit the future investigation of the immunopathogenesis of persistent inflammation following C trachomatis infection.
authors.affiliation: Department of Genito-urinary Medicine and Communicable Diseases, Imperial College School of Medicine at St Mary's, London.
authors.forename: P J
authors.initials: PJ
authors.lastname: Horner
authors.forename: M
authors.initials: M
authors.lastname: Ali
authors.forename: D
authors.initials: D
authors.lastname: Parker
authors.forename: J N
authors.initials: JN
authors.lastname: Weber
authors.forename: D
authors.initials: D
authors.lastname: Taylor-Robinson
authors.forename: M O
authors.initials: MO
authors.lastname: McClure
mesh_terms: D015153:Blotting, Western; D018834:Chaperonin 60 / Q000097:blood*; D002690:Chlamydia Infections / Q000097:blood*; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase
chemical_list: D018834:Chaperonin 60; D001616:beta-Galactosidase