Chemiluminescence enzyme immunoassay using bacterial magnetic particles.
A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio) propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-10(5) fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration in the range of 10-1000 ng/mL.
Matsunaga
T
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Kawasaki
M
M
Yu
X
X
Tsujimura
N
N
Nakamura
N
N
D000469:Alkaline Phosphatase / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D003432:Cross-Linking Reagents; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D008163:Luminescent Measurements; D008280:Magnetics; D008301:Maleimides; D051379:Mice; D013139:Spirillum / Q000737:chemistry; D013388:Succinimides
D000906:Antibodies; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D008301:Maleimides; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; C025285:N-(4-carboxycyclohexylmethyl)maleimide N-hydroxysuccinimide ester; D000469:Alkaline Phosphatase