title: Primary structure of bovine adenosine deaminase.
abstract: Derivatized bovine adenosine deaminase is used in enzyme replacement therapy and as an adjunct to gene therapy against severe combined immunodeficiency syndrome. Although a gene sequence is known for human adenosine deaminase, the structure of the bovine enzyme has not been characterized. Structure studies using mass spectrometry are reported here that evaluate sequence, processing, post-translational modifications and the extent of homology between the human protein and its therapeutic surrogate.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Kelly
authors.forename: M M
authors.initials: MM
authors.lastname: Vestling
authors.forename: C M
authors.initials: CM
authors.lastname: Murphy
authors.forename: S
authors.initials: S
authors.lastname: Hua
authors.forename: T
authors.initials: T
authors.lastname: Sumpter
authors.forename: C
authors.initials: C
authors.lastname: Fenselau
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D000243:Adenosine Deaminase