title: Matrix metalloproteinase expression during mouse peri-implantation development.
abstract: OBJECTIVE
  The purpose of this study was to define the temporal expression and to quantitate the mRNA levels of collagenase, 72 kDa, 92 kDa, and membrane-type matrix metalloproteinase during the peri-implantation period of pregnancy in the mouse uterus. Embryonic expression of 72 kDa and 92 kDa matrix metalloproteinases, as well as interleukin 1 alpha, was also investigated.
  
  
  METHODS
  Uterine matrix metalloproteinases were detected using gelatin substrate gel electrophoresis (zymography) and reverse-transcription polymerase chain reaction methodology was used to detect and quantitate different mRNA species in the mouse uterus and blastocyst.
  
  
  RESULTS
  Collagenase, 72 kDa, and 92 kDa matrix metalloproteinases are developmentally regulated during the peri-implantation period of pregnancy, but membrane-type matrix metalloproteinase appears to be expressed constitutively. Matrix metalloproteinase mRNA levels have been quantitated and confirm the observed developmental expression patterns. Prominent expression of bot 92 kDa matrix metalloproteinase and interleukin 1 alpha was observed in blastocysts during outgrowth while weak expression of the 72 kDa matrix metalloproteinase was detected.
  
  
  CONCLUSIONS
  The date provide evidence of matrix metalloproteinase expression in vivo and substantiate their potential role in tissue remodeling prior to and during blastocyst implantation. Expression of interleukin 1 alpha, 92 kDa, and 72 kDa matrix metalloproteinases suggests that these proteins are important for trophoblast invasion associated with implantation of the early embryo.
authors.affiliation: Department of Biology, Clark University, Worcester, Massachusetts, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Sharkey
authors.forename: R R
authors.initials: RR
authors.lastname: Adler
authors.forename: G L
authors.initials: GL
authors.lastname: Nieder
authors.forename: C A
authors.initials: CA
authors.lastname: Brenner
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001755:Blastocyst / Q000201:enzymology / Q000378:metabolism; D017364:Collagenases / Q000096:biosynthesis* / Q000235:genetics; D010064:Embryo Implantation / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D018093:Gelatinases / Q000096:biosynthesis / Q000235:genetics; D007375:Interleukin-1 / Q000096:biosynthesis; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D017364:Collagenases; D018093:Gelatinases; D053509:Matrix Metalloproteinase 13; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; C507081:Mmp13 protein, mouse; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9