Transcytosis of lipid microspheres by human endothelial cells.
Human endothelial cells were cultivated on microporous membranes mimicking the luminal and basal spaces of blood vessels. When fluorescence-labeled lipid microspheres (LM) were added to the upper chambers of the model cultures, confluent monolayers of endothelial cells transported considerable levels of fluorescence to lower chambers. The transport was time dependent and was diminished by the addition of cytochalasin B. The uptake of LM into the endothelial cytoplasm was confirmed by electron microscopy and laser scanning confocal imaging. The amounts of fluorescence in the lower chamber were reduced when the endothelial cell layer was fixed with formaldehyde. These observations suggest that endothelial cells can transport LM by transcytosis. Endothelial cells seem to carry the LM without processing, since only minimal amounts of free fluorescence were detected even after longer cultivation periods. The fluorescence in the lower chambers of cell cultures treated with interleukin 1 beta was 3.7-fold higher than that of untreated cells; interleukin 2 and tumor necrosis factor alpha treatments had no discernible effect on LM transport. The interleukin 1 beta induced increase of transcytosis in endothelial cells would explain why LM preferentially accumulate in inflammatory tissues.
Takahashi
K
K
Department of Biochemistry, National Defence Medical College, Saitama, Japan.
Suzuki
K
K
Ichiki
Y
Y
Fukushima
T
T
Nakamura
H
H
Sawasaki
Y
Y
D001692:Biological Transport; D002478:Cells, Cultured; D003911:Dextrans / Q000737:chemistry; D016503:Drug Delivery Systems; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D050356:Lipid Metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008863:Microspheres; D016062:Porosity; D013050:Spectrometry, Fluorescence; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D003911:Dextrans; D005456:Fluorescent Dyes; D007375:Interleukin-1; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D016650:Fluorescein-5-isothiocyanate