title: Acoustically activated c-fos expression in auditory nuclei of the anaesthetised guinea pig.
abstract: The spatial expression of the immediate-early gene c-fos in central auditory nuclei of the anaesthetised guinea pig was investigated following exposure of the animal to acoustic stimulation. Accurate control of both the spectra and the level of the stimulus was designed so that the presumed excitation of central auditory nuclei was similar across animals. For unstimulated anaesthetised control animals levels of labelling were significantly higher when compared with unanaesthetised controls. This appeared to a result of the combination of the experimental manipulations and also the use of the anaesthetic. A surprising finding was that unstimulated control animals placed in an anechoic chamber demonstrated the highest levels of fos-like immunoreactivity (Fos-LIR). When anaesthetised animals were exposed to acoustic stimuli the total number of cells showing Fos-LIR was elevated when compared to anaesthetised, but unstimulated animals. There was no evidence at any level of the auditory pathway that these animals demonstrated spatially restricted Fos-LIR which may have suggested place-frequency mapping. In contrast, spatially restricted labelling was found in awake animals exposed to an identical stimulus.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, Australia. a.cody@mailbox.uq.oz.au
authors.forename: A R
authors.initials: AR
authors.lastname: Cody
authors.forename: W
authors.initials: W
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Leah
mesh_terms: D000161:Acoustic Stimulation; D000777:Anesthetics; D000818:Animals; D001306:Auditory Pathways / Q000378:metabolism; D001931:Brain Mapping; D001933:Brain Stem / Q000378:metabolism*; D002452:Cell Count; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012016:Reference Values
chemical_list: D000777:Anesthetics; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos