title: Evidence of induced non-tolerance in HLA-identical twins with hemoglobinopathy after in utero fetal transplantation.
abstract: Fetus-to-fetus transplantation has been suggested for the treatment of hemoglobinopathies in utero. However, dissimilar results have to date been obtained by different groups. We describe a case in which fetus-to-fetus transplantation in HLA-identical twins was performed at the 19th week of gestation by infusion of 0.8 ml of fetal blood from normal to beta-thalassemia affected fetus with the main aim of inducing tolerance. No evidence of engraftment, determined by KM19 polymorphism, was present after 2 years of the procedure. Moreover, an alloreactive cytotoxic T lymphocyte precursor (CTLp) study of affected fetus vs donor and other different stimulators showed that immunization vs tolerance was the real effect of the procedure.
authors.affiliation: Servizio Talassemia e Diagnosi Prenatale, Unita' di Ricerca Piera Cutino, Ospedale V Cervello, Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Orlandi
authors.forename: A
authors.initials: A
authors.lastname: Giambona
authors.forename: F
authors.initials: F
authors.lastname: Messana
authors.forename: M
authors.initials: M
authors.lastname: Marino
authors.forename: I
authors.initials: I
authors.lastname: Abate
authors.forename: R
authors.initials: R
authors.lastname: Calzolari
authors.forename: F
authors.initials: F
authors.lastname: Damiani
authors.forename: C
authors.initials: C
authors.lastname: Jakil
authors.forename: M
authors.initials: M
authors.lastname: Renda
authors.forename: F
authors.initials: F
authors.lastname: Dieli
authors.forename: F
authors.initials: F
authors.lastname: Buscemi
authors.forename: M
authors.initials: M
authors.lastname: Westgren
authors.forename: O
authors.initials: O
authors.lastname: Ringden
authors.forename: A
authors.initials: A
authors.lastname: Maggio
mesh_terms: D000293:Adolescent; D005260:Female; D005330:Fetofetal Transfusion; D006650:Histocompatibility Testing; D006801:Humans; D007108:Immune Tolerance; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D017086:beta-Thalassemia / Q000628:therapy*