Sensitive enzyme-linked immunosorbent assay for adult T-cell leukemia-derived factor and normal value measurement.
Four different monoclonal antibodies against recombinant adult T-cell leukemia-derived factor (ADF), identical to thioredoxin, were established and used for the determination of ADF concentration in serum. Using two of the monoclonal antibodies, we developed a two-step enzyme-linked immunosorbent assay (ELISA) for ADF. This ELISA showed a highly specific reactivity on ADF with no cross-reactivity to several proteins with homologue sequence on the active center. The detection limit of the assay was 2.0 ng/ml (mean +/- 2 SD). The intra- and interassay coefficients of variation (CV) were 0.81-3.74% (n = 8) and 4.78-6.97% (n = 7), respectively. The normal value of ADF mean concentration from 145 healthy donors was 40.8 ng/ml.
Kogaki
H
H
D2 Department, Fujirebio, Inc., Tokyo, Japan.
Fujiwara
Y
Y
Yoshiki
A
A
Kitajima
S
S
Tanimoto
T
T
Mitsui
A
A
Shimamura
T
T
Hamuro
J
J
Ashihara
Y
Y
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D012016:Reference Values
D000911:Antibodies, Monoclonal; D016207:Cytokines; D009363:Neoplasm Proteins; C055533:adult T cell leukemia-derived factor