Fat emulsions based on structured lipids (1,3-specific triglycerides): an investigation of the in vivo fate.
OBJECTIVE
Structured lipids (1,3-specific triglycerides) are new chemical entities made by enzymatic transesterification of the fatty acids in the 1,3 positions of the triglyceride. The purpose of this study is to characterize structured lipids with either short chain fatty acids or medium chain fatty acids in the 1,3 positions with regard to their hydrophobicity, and investigate the in vivo fate in order to evaluate the potential of structured lipids as core material in fat emulsions used as parenteral drug delivery system.


METHODS
The lipids were characterized by employing reversed phase high performance liquid chromatography. The biodistribution of radioactively labeled emulsions was studied in rats.


RESULTS
By employing high performance liquid chromatography a rank order of the hydrophobicities of the lipids could be given, with the triglycerides containing long chain fatty acids being the most hydrophobic and the structured lipid with short chain fatty acids in the 1,3 positions the least. When formulated as fat emulsions, the emulsion based on structured lipids with short fatty acids in the 1,3 positions was removed slower from the general blood circulation compared to emulsions based on lipids with long chain fatty acids in the 1,3 positions.


CONCLUSIONS
The type of core material influences the in vivo circulation time of fat emulsions.
Hedeman
H
H
Department of Pharmaceutics, Royal Danish School of Pharmacy, Universitetsparken, Copenhagen, Denmark.
Brøndsted
H
H
Müllertz
A
A
Frokjaer
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004655:Emulsions / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000737:chemistry / Q000493:pharmacokinetics*
D004655:Emulsions; D014280:Triglycerides