Single-strand conformation polymorphism analysis of RT-PCR products of UK isolates of barley yellow mosaic virus.
Single-strand conformation polymorphism (SSCP) analysis of the bipartite genomes of several UK isolates of barley yellow mosaic virus (Ba YMV) was done using fragments of cDNA amplified by RT-PCR. Isolates differed in their SSCP patterns in several regions, but in no case was the pattern able to distinguish between common and resistance-breaking strains. In regions where the nucleotide sequences of UK isolates had been determined, there was no simple relationship between numbers of nucleotide differences and SSCP patterns: differences of only 2 or 3 nucleotides (nt) gave different SSCP patterns, whereas differences of as many as 29 nt did not. Although SSCP analysis has some potential as a rapid and sensitive tool for distinguishing virus isolates, differences detected do not necessarily relate to biological properties and the results are highly dependent on gel conditions.
Shi
N N
NN
Scottish Crop Research Institute, Imergowrie, Dundee, UK.
Chen
J
J
Wilson
T M
TM
Macfarlane
S A
SA
Antoniw
J F
JF
Adams
M J
MJ
D000180:Acrylic Resins; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005782:Gels; D001467:Hordeum / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D006113:United Kingdom
D000180:Acrylic Resins; D004279:DNA, Viral; D005782:Gels; C016680:polyacrylamide gels