title: Upstream sequence of the parahydroxyphenyl-pyruvate dioxygenase (melA) coding region leading to enhanced expression in Shewanella colwelliana.
abstract: Hyperexpression of the enzyme, parahydroxyphenylpyruvate dioxygenase (pHPPH; melA), in the tyrosine degradative pathway yields excess homogentisic acid which oxidatively polymerizes to pyomelanin. Depression of melA in Shewanella colwelliana strain D was found to result from a single base pair transition upstream of the promoter. This was the sole lesion detected in pHPPH hyperexpressing strains. It is suggested that a T to C transition alters the mRNA structure, exposing the ribosome binding site, thereby enhancing translational efficiency.
authors.affiliation: Department of Microbiology, University of Maryland, College Park 20742, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Eylem
authors.forename: E J
authors.initials: EJ
authors.lastname: Quintero
authors.forename: R M
authors.initials: RM
authors.lastname: Weiner
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000201:enzymology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D015111:4-Hydroxyphenylpyruvate Dioxygenase