[Adaptation of the measurement of plasma and erythrocyte glutathione peroxidase activity on Hitachi 911].
The aim was to automate the assay of plasma and erythrocyte glutathione peroxidase (GSH-Px) activity using the Hitachi 911 (Boehringer Mannheim). There is a need for a reliable and rapid technique for the determination of GSH-Px activity to evaluate 'the oxidative stress'. The technique used was that proposed by the Randox laboratories (Randox Laboratories Ltd, Ransel test, reference: RS-505). The Hitachi 911 was programmed by adapting the manual Randox technique. Repeatability, reproducibility and linearity were satisfactory, and usual values for plasma and erythrocyte GSH-Px activity were determined. Automation of the Ransel test for determination of GSH-Px activity was very useful for serial assays. In addition, the technique is practical and has good interlaboratory reproducibility.
Dever
S
S
Laboratoire de biochimie, hôpital de la Salpêtrière, Paris, France.
Vegas
C
C
Imbert-Bismut
F
F
Delattre
J
J
Bonnefont-Rousselot
D
D
D001774:Blood Chemical Analysis / Q000379:methods*; D002624:Chemistry, Clinical / Q000295:instrumentation*; D004912:Erythrocytes / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
D005979:Glutathione Peroxidase