Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast.
The bacterial gene, encoding alpha-acetolactate decarboxylase (alpha-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of alpha-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. alpha-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.
Onnela
M L
ML
VTT Biotechnology and Food Research, Espoo, Finland.
Suihko
M L
ML
Penttilä
M
M
Keränen
S
S
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D001515:Beer / Q000592:standards; D015152:Blotting, Northern; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
D011994:Recombinant Proteins; D000426:Alcohol Dehydrogenase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase