Simultaneous immunoselection in vitro for H-Y or H-2D antigen-loss variants of a mouse-derived B cell line.
Cytotoxic T lymphocytes (CTL) specifically reactive with the male transplantation antigen (H-Y) were used to immunoselect in vitro for antigen loss among cells from an Abelson murine leukemia virus (AbMuLV) transformed lymphoblastoid cell line. Numerous variant cell clones were recovered that had lost expression of either H-Y or the restricting major histocompatibility class I molecule, H-2D. In all experiments, low-level gamma-irradiation applied prior to immunoselection increased the frequency of antigen loss, but when different time intervals between mutagenesis and immunoselection were used, the proportion of H-Y to H-2D antigen loss was affected, suggesting that the antigens selected against remain on the surface of the cell for differing amounts of time following allele loss.
King
T R
TR
Biological Sciences, Central Connecticut State University, New Britain 06050, USA.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects; D002461:Cell Line, Transformed; D005260:Female; D006183:H-2 Antigens / Q000235:genetics* / Q000528:radiation effects; D006182:H-Y Antigen / Q000235:genetics* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009154:Mutation
D006183:H-2 Antigens; D006182:H-Y Antigen