Development of a diagnostic DNA probe to detect Setaria digitata: the causative parasite of cerebrospinal nematodiasis in goats, sheep and horses.
Two repetitive sequences (IpSdM and IpSdS) have been cloned and sequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the S. digitata genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both S. digitata and S. labiato-papillosa. It can also detect a single microfilaria and a L3 larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.
Wijesundera
W S
WS
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka.
Chandrasekharan
N V
NV
Karunanayake
E H
EH
Dharmasena
S P
SP
D000818:Animals; D015342:DNA Probes; D015511:Goat Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*; D006041:Goats; D006734:Horse Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*; D006736:Horses; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D017182:Setaria Nematode / Q000235:genetics*; D012719:Setariasis / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D012756:Sheep; D012757:Sheep Diseases / Q000134:cerebrospinal fluid / Q000469:parasitology*
D015342:DNA Probes