Distribution of concanavalin A and wheat germ agglutinin binding sites in the rat peripheral nerve fibres revealed by lectin/glycoprotein-gold histochemistry.
The affinity of rat peripheral nerve fibres for concanavalin A (Con A) and wheat germ agglutinin (WGA) was tested in semi-thin sections of Epon-embedded material. A two-step post-embedding technique was used. As a first step, Con A and WGA were used in pure form. As a second step, peroxidase-gold (for Con A) and ovomucoid-gold (for WGA) complexes were applied. The lectin-binding sites, visualized by means of signal amplification with the photochemical silver reaction, were associated mainly with the myelin sheaths and the surfaces of Schwann cells.
Dolapchieva
S
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Department of Anatomy and Histology, Medical University, Sofia, Bulgaria.
D000818:Animals; D001665:Binding Sites; D003208:Concanavalin A / Q000378:metabolism*; D004853:Epoxy Resins / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D037102:Lectins / Q000737:chemistry; D009186:Myelin Sheath / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism*; D010059:Ovomucin / Q000737:chemistry / Q000378:metabolism; D017933:Peripheral Nervous System / Q000378:metabolism; D016613:Plastic Embedding; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D016622:Silver Staining; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
D004853:Epoxy Resins; D006023:Glycoproteins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D010059:Ovomucin; C004875:EPON