Construction of cDNA library from liver RNA of heat shocked rats and DNA sequence analysis of the clone containing the 3'-end untranslated region (3'UTR) of the heat inducible gene hsp70.2.
cDNA library constructed from liver RNA of rats subjected to hyperthermia was used to isolate divergent 3' end untranslated regions (3'UTR) of heat inducible hsp70.1 and hsp70.2 genes. As a result of a double selection procedure with the use of DNA-DNA hybridization and PCR analysis 9 clones containing cDNA sequences derived from the 3'UTR of the hsp70.2 gene were selected. Nucleotide sequence of the cloned inserts was established and the Northern blot analysis was performed to identify the heat inducible transcript encoded by the hsp70.2 gene.
Lisowska
K
K
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Scieglińska
D
D
Krawczyk
Z
Z
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018840:HSP70 Heat-Shock Proteins; C490435:Hspa2 protein, rat; D012313:RNA