title: Characterization of genes from Thermoanaerobacterium thermosulfurigenes EM1 that encode two glycosyl hydrolases with conserved S-layer-like domains.
abstract: Two genes from Thermoanaerobacterium thermosulfurigenes EM1 were identified which are predicted to encode a xylanase (XynA) and a polygalacturonate hydrolase (Pg1A). The xynA gene has the potential to encode a 1234-amino acid product consisting of a signal peptide followed by a repeated domain, a xylanase family F domain, two cellulose-binding domains and a triplicated sequence at its C-terminus. The gene pglA is predicted to encode a product of 1148 amino acids consisting of a signal sequence followed by a fibronectin type III-like domain (Fn3 domain), the catalytic domain, a Gly/Thr/Ser/Asn-rich segment and a triplicated domain. The triplicated segments at the C-termini of deduced XynA and Pg1A are about 95% identical to each other and to the S-layer-like domains of the previously characterized pullulanase (AmyB) from the same organism. In contrast, sequence comparisons revealed only distant amino acid sequence similarities between the fibronectin type III-like domains of Pg1A and AmyB from T. thermosulfurigenes EM1.
authors.affiliation: Institut für Mikrobiologie, Georg-August-Universität Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Matuschek
authors.forename: K
authors.initials: K
authors.lastname: Sahm
authors.forename: A
authors.initials: A
authors.lastname: Zibat
authors.forename: H
authors.initials: H
authors.lastname: Bahl
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence; D043364:Endo-1,4-beta Xylanases; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014995:Xylosidases / Q000235:genetics*
chemical_list: D006026:Glycoside Hydrolases; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases; C065616:exo-poly-alpha-galacturonosidase