title: Analysis of human papillomavirus type 16 E5 oncogene expression in vitro and from bicistronic messenger RNAs.
abstract: During a human papillomavirus infection (HPV), a set of spliced messenger RNAs (mRNA) are produced from the coding strand of the 8-kilobase virus DNA genome. These mRNAs have been isolated from cervical intraepithelial neoplasia biopsies, tumor biopsies and HPV type 16 (HPV-16)-containing cell lines, and subsequent analyses have shown that the different mRNAs are obtained by often complex splicing events. In the present study we have investigated the coding capacity of the HPV-16 E5 open reading frame (ORF) in an in vitro translation system and by the expression of a HPV-16 E5-CAT fusion gene from bicistronic messenger RNAs derived from polycistronic mRNAs which have previously been identified by others. Our results show that the HPV-16 E5 protein synthesis can only be initiated from the authentic ATG codon in the presence of E2 ORF translation. The E5 gene is found to be expressed only from a bicistronic mRNA with an E2 ORF upstream of the E5 gene indicating that the E5 protein is synthesized coordinated with the E2 protein. The supposition that the E5 protein is synthesized early in infection is discussed.
authors.affiliation: Protein Laboratory, Panum Institute, Copenhagen, Denmark.
authors.forename: C K
authors.initials: CK
authors.lastname: Johnsen
authors.forename: M
authors.initials: M
authors.lastname: Stanley
authors.forename: B
authors.initials: B
authors.lastname: Norrild
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005796:Genes; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D000086082:Viroporin Proteins
chemical_list: D015500:Chloramphenicol O-Acetyltransferase; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins