A defect in inducible beta-galactosidase of B lymphocytes in the osteopetrotic (mi/mi) mouse.
Macrophages were activated by administration of an inflammatory lipid metabolite, lysophosphatidylcholine (lyso-Pc), to wild type mice but not murine (microphthalmic) osteopetrotic (mi/mi) mutant mice. In vitro treatment of wild type mouse peritoneal cells with lyso-Pc efficiently activated macrophages whereas lyso-Pc-treatment of mi mutant mouse peritoneal cells resulted in no activation of macrophages. Generation of macrophage activating factor requires a precursor protein, serum vitamin D binding protein (DBP), and participation of lyso-Pc-inducible beta-galactosidase of B lymphocytes. Lyso-Pc-inducible beta-galactosidase of B lymphocytes was found to be defective in mi mutant mice.
Yamamoto
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Laboratory of Cancer Immunology and Molecular Biology, Albert Einstein Cancer Center, Philadelphia, PA 19141, USA.
Naraparaju
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VR
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D018929:Cell Culture Techniques; D008244:Lysophosphatidylcholines / Q000276:immunology; D008262:Macrophage Activation / Q000276:immunology*; D016215:Macrophage-Activating Factors / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D010022:Osteopetrosis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D008244:Lysophosphatidylcholines; D016215:Macrophage-Activating Factors; D001616:beta-Galactosidase