Bradykinin release from high molecular weight kininogen in surgical ICU patients.
The purpose of this prospective study was to characterize kininogens in plasma from surgical ICU patients. Thirty-five patients, ages 19-79 years, were divided into 2 groups: sepsis (defined by standard criteria) and nonsepsis. Studies of proteolytic degradation of H-kininogen showed degradation in both patient groups compared to healthy controls. Functional quantification of prekallikrein showed a reduction of prekallikrein in plasma from both patient groups. Functional quantification of kininogens by a CPI (cysteine proteinase inhibitor) assay showed no significant differences between the patients and the controls. Immunological levels of H-kininogen and total kininogen were not significantly different from normal plasma. No differences could be detected between the two patient groups in any of the parameters studied. In conclusion, this study supported contact activation taking place in surgical ICU patients, a partial kinin release and a consumption of prekallikrein has taken place in vivo.
Karlsrud
T S
TS
Department of Pharmacology, School of Pharmacy, University of Oslo, Norway. t.s.karlsrud@farmasi.uio.no
Buø
L
L
Aasen
A O
AO
Johansen
H T
HT
D000328:Adult; D000368:Aged; D001920:Bradykinin / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D003422:Critical Care; D005260:Female; D006801:Humans; D007704:Kininogens / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011446:Prospective Studies
D007704:Kininogens; D001920:Bradykinin