title: Contrasting migratory response of astrocytoma cells to tenascin mediated by different integrins.
abstract: Tenascin, an extracellular matrix protein, is expressed in human gliomas in vitro and in vivo. The distribution of tenascin at the invasive edge of these tumors, even surrounding solitary invading cells, suggests a role for this protein as a regulator of glioma cell migration. We tested whether purified tenascin, passively deposited on surfaces, influenced the adhesion or migration of a human gliomaderived cell line, SF-767. Adhesion of glioma cells to tenascin increased in a dose-dependent fashion up to a coating concentration of 10 micrograms/ml. Higher coating concentrations resulted in progressively fewer cells attaching. Cell adhesion could be blocked to basal levels using anti-beta 1 integrin antibodies. In contrast, when anti-alpha v antibodies were added to the medium of cells on tenascin, cell adhesion was enhanced slightly. Using a microliter scale migration assay, we found that cell motility on tenascin was dose dependently stimulated at coating concentrations of 1 and 3 micrograms/ml, but migration was inhibited below levels of non-specific motility when tested at coating concentrations of 30 and 100 micrograms/ml. Migration on permissive concentrations of tenascin could be reversibly inhibited with anti-beta 1, while treatment with anti-alpha v antibodies increased migration rates. We conclude that SF-767 glioma cells express two separate integrin receptors that mediate contrasting adhesive and migratory responses to tenascin.
authors.affiliation: Department of Neurosurgery, University Hospital Eppendorf, Hamburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Giese
authors.forename: M A
authors.initials: MA
authors.lastname: Loo
authors.forename: S A
authors.initials: SA
authors.lastname: Norman
authors.forename: S
authors.initials: S
authors.lastname: Treasurywala
authors.forename: M E
authors.initials: ME
authors.lastname: Berens
mesh_terms: D001254:Astrocytoma / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D002448:Cell Adhesion; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D007797:Laminin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D019063:Tenascin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D019096:Vitronectin / Q000494:pharmacology
chemical_list: D016023:Integrins; D007797:Laminin; D019063:Tenascin; D019096:Vitronectin