In vivo association of protein fragments giving active AraC.
Frameshift mutations in a restricted portion of the arabinose operon regulatory gene araC from Escherichia coli give rise to active AraC protein, likely from the in vivo synthesis of two incomplete fragments that are active together. Synthesis of corresponding fragments, each separately inactive, from two plasmids within cells also resulted in complementation.
Eustance
R J
RJ
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Schleif
R F
RF
D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D016368:Frameshift Mutation; D005816:Genetic Complementation Test; D010957:Plasmids; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors