Interleukin-1 beta activates PI 3-kinase in renal mesangial cells.
Interleukin-1 beta rapidly stimulates the activation of phosphatidylinositol 3-hydroxyl kinase in renal mesangial cells. This interleukin-1 beta dependent activation is associated with a rapid increase in phosphatidyl inositol 3,4,5 phosphate in renal mesangial cells. The increase in PI 3-kinase activity is inhibited by wortmannin. In addition wortmannin partially inhibited IL-1 beta induced PGE2 production and potentiated IL-1 beta induced nitric oxide production. These experiments suggest that IL-1 beta can activate PI 3-kinase in renal mesangial cells and that the enzyme plays a role in IL-1 beta induced PGE2 and NO formation in the renal mesangial cell.
Donaldson
A
A
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Daphna-Iken
D
D
Tetsuka
T
T
Morrison
A R
AR
D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077191:Wortmannin
D000730:Androstadienes; D004791:Enzyme Inhibitors; D007375:Interleukin-1; D009569:Nitric Oxide; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D015232:Dinoprostone; D000077191:Wortmannin