Alternate functions of the single-minded and rhomboid genes in development of the Drosophila ventral neuroectoderm.
We have investigated the roles of the single-minded (sim) and rhomboid (rho) genes in generating distinct cell fates in the Drosophila embryonic neuroectoderm. We show that sim functions to repress ventral ectodermal cell fates, as in sim mutants mesectodermal cells adopt the fates of neighboring ventral ectodermal cells and targeted sim expression in P[paired.Gal4]/P[UAS-sim] embryos results in loss of epidermal cells. We also find that rho is not required for early expression of sim or ventral nervous system defective in mesectodermal or ventral ectodermal cells; targeted rho expression in P[paired-Gal4]/P[UAS-rho] embryos results in lateral-to-ventral cell fate shifts in the developing neuroectoderm; and midline-targeted rho expression can rescue the medial denticle fusions in rho mutant cuticles.
Xiao
H
H
Program for Molecular and Cellular Biology, University of Massachusetts at Amherst 01003, USA.
Hrdlicka
L A
LA
Nambu
J R
JR
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins; D004475:Ectoderm / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D018257:Helix-Loop-Helix Motifs / Q000235:genetics / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000235:genetics / Q000502:physiology; D019476:Insect Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D051155:Wnt1 Protein
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D051155:Wnt1 Protein; C081481:Rho protein, Drosophila; C472498:Stet protein, Drosophila; C089449:sim protein, Drosophila; C074622:wg protein, Drosophila; C077245:hh protein, Drosophila