Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis.
The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtraction are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, 1993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
Ermolaeva
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Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Sverdlov
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D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D016678:Genome; D006801:Humans; D007700:Kinetics; D051379:Mice; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013382:Subtraction Technique; D014998:Y Chromosome
D004247:DNA