title: Noncytopathogenic and cytopathogenic bovine viral diarrhea-mucosal disease viruses do not affect in vitro embryonic development into the blastocyst stage.
abstract: Bovine oocytes enclosed within follicular epithelial (FE) cells were exposed to noncytopathogenic or cytopathogenic strains of bovine viral diarrhea-mucosal disease virus (BVD-MDV). After culture at 39 degrees C in humidified air with 5 percent CO2 for 24 h, the oocytes matured and then in vitro fertilization was performed. Some of the fertilized oocytes developed into blastocysts from day 8 to day 10 (day 1: date of insemination), and the rate of development to blastocysts was the same as for the unexposed control oocytes. In the developmental medium, each strain of BVD-MDV was present at 10(3) to 10(6) TCID (50)/0.25 ml from day 3 to day 10, respectively and the virus was isolated from the FE cells at high titers and from the embryos at low titers on day 10. BVD-MDV antigen was detected in the cytoplasm of the FE cells by indirect immunofluorescence. These findings indicate that BVD-MDV replicates well in FE cells. The results of this study suggest that BVD-MDV replication in cells around embryos has no effect on bovine embryo development.
authors.affiliation: Tohoku Branch Laboratory, National Institute of Animal Health, 31, Uminai, Shichinohe, Kamikita, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuboi
authors.forename: T
authors.initials: T
authors.lastname: Imada
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000472:pathogenicity*; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D009865:Oocytes / Q000166:cytology / Q000502:physiology* / Q000821:virology; D013997:Time Factors