Detection and measurement of Staphylococcus epidermidis slime using an ELISA technique.
A polyclonal rabbit anti-serum against the strong slime-producing Staphylococcus epidermidis strain RP62A was absorbed with the slime-negative phase variant of this strain PV1 in order to remove not slime-specific antibodies. Using this antiserum we established an ELISA which enables detection of slime production in S. epidermidis extracts. The ELISA showed high absorbance when extracts from slime-positive strains (confirmed in the tissue culture tube test) were used as antigens. The high absorbance of slime-positive strains was greatly reduced by periodate oxidation of the extracts and was resistant to proteinase digestion suggesting that the detected antigen is composed of polysaccharides. In contrast to other rapid and simple laboratory detection methods for S. epidermidis slime, the slime-specific ELISA gave positive results in the presence of human serum.
Mempel
M
M
Institut für Medizinische Mikrobiologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Günther
B
B
Müller
E
E
Grüter
L
L
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D010450:Endopeptidases / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000378:metabolism; D011817:Rabbits; D015203:Reproducibility of Results; D013212:Staphylococcus epidermidis / Q000737:chemistry / Q000302:isolation & purification*
D001426:Bacterial Proteins; D010504:Periodic Acid; C009288:metaperiodate; D010450:Endopeptidases