title: Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide.
abstract: Recently, it has been shown that the lipopolysaccharide (LPS) O antigen of Helicobacter pylori contains Lewis x (Lex), Lewis y (Ley), or both Lex and Ley antigens. We applied a serotyping method for H. pylori by an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) specific for these antigens and the related fucosylated H type 1 (H1) antigen. The selected MAbs recognized the Lex and/or Ley structures in the LPS of H. pylori. The agreement between the results of biochemical compositional analysis and the serological data validated our serotyping system. A total of 152 strains from different geographic origins (The Netherlands, Canada, Poland, Italy, and People's Republic of China) were examined for typeability based on the presence of Lewis antigens. One hundred twenty-nine (84.9%) strains were typeable, and 12 different serotyping patterns were observed; 80.9% of the strains contained Lex and/or Le(y) antigens, and 18.4% reacted with the MAb against the related H1 antigen either alone or in combination with the Lex and/or Ley antigen. Our results show that the Lex and Ley antigens are frequently encountered in the LPS of H. pylori strains from various geographic origins. This typing method is an easy-to-perform technique, which can be used for strain differentiation in epidemiological studies of H. pylori infections.
authors.affiliation: Department of Medical Microbiology, School of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: I M
authors.initials: IM
authors.lastname: Simoons-Smit
authors.forename: B J
authors.initials: BJ
authors.lastname: Appelmelk
authors.forename: T
authors.initials: T
authors.lastname: Verboom
authors.forename: R
authors.initials: R
authors.lastname: Negrini
authors.forename: J L
authors.initials: JL
authors.lastname: Penner
authors.forename: G O
authors.initials: GO
authors.lastname: Aspinall
authors.forename: A P
authors.initials: AP
authors.lastname: Moran
authors.forename: S F
authors.initials: SF
authors.lastname: Fei
authors.forename: B S
authors.initials: BS
authors.lastname: Shi
authors.forename: W
authors.initials: W
authors.lastname: Rudnica
authors.forename: A
authors.initials: A
authors.lastname: Savio
authors.forename: J
authors.initials: J
authors.lastname: de Graaff
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D015373:Bacterial Typing Techniques; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens; D008070:Lipopolysaccharides