Localization of sites of enhanced expression of endothelin-1 in the kidney of DOCA-salt hypertensive rats.
Although the role of endothelin-1, a potent vasoconstrictor peptide, in hypertension remains unclear, there is evidence of its involvement in deoxycorticosterone acetate (DOCA)-salt hypertensive rats, in which enhanced vascular production of endothelin-1 has been documented. The study presented here examined endothelin-1 gene expression in the kidney in DOCA-salt hypertensive rats by in situ hybridization histochemistry. A high specific activity 35S-labeled complementary RNA probe was used. Significant increases in abundance of endothelin-1 mRNA transcripts were found in the endothelium of renal vessels, and in capillary endothelial and mesangial cells of glomeruli of the remaining kidney of DOCA-salt hypertensive rats, in comparison with unilaterally nephrectomized control rats. Enhanced expression of the endothelin-1 gene in the kidney of DOCA-salt hypertensive rats may participate in abnormalities of renal function in this model of hypertension, and thus contribute to the development and maintenance of elevated blood pressure.
Deng
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Medical Research Council Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montréat, University of Montréal, Québec, Canada.
Day
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Schiffrin
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D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D003900:Desoxycorticosterone; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D005920:Glomerular Mesangium / Q000378:metabolism; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism*; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009392:Nephrectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000633:toxicity
D019332:Endothelin-1; D012333:RNA, Messenger; D003900:Desoxycorticosterone; D012965:Sodium Chloride