Formation of O6-ethylguanine in spermatogonial DNA of adult Syrian golden hamster by intraperitoneal injection of diethylnitrosamine.
17-Week-old Syrian golden hamsters received a single intraperitoneal injection of diethylnitrosamine (DEN) at a dose of 100 mg/kg body weight. The DNA alkylation product O6-ethylguanine was formed in the spermatogonia. The data demonstrate that DEN can pass the blood-testis barrier and be metabolized in the spermatogonia to yield an alkylating derivative or that externally activated metabolites themselves can pass the barrier.
Kamino
K
K
Institute of Experimental Pathology, Hannover Medical School, Germany.
Seiler
F
F
Emura
M
M
Thomale
J
J
Rajewsky
M F
MF
Mohr
U
U
D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism*; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D008297:Male; D008647:Mesocricetus; D013093:Spermatogonia / Q000187:drug effects* / Q000378:metabolism*
D018736:DNA Adducts; D004052:Diethylnitrosamine; C007673:6-ethylguanine; D006147:Guanine; D004247:DNA