title: Porcine adenoviruses types 1, 2 and 3 have short and simple early E-3 regions.
abstract: The nucleotide sequence of the E-3 region genes, the hexon associated protein pVIII genes, and part of the fiber genes coding for the N-terminal tail regions, of porcine adenovirus (PAV) types 1 and 2 were determined. The sizes of the E-3 regions were found to be 1162 and 1222 bp, respectively. The five open reading frames (ORF) encoded within the sequenced regions of PAV types 1 and 2 shared a high degree of homology with the published sequences of the corresponding ORFs of PAV-3. The E-3 regions of PAV types 1, 2 and 3 were further characterized by Northern blot analysis and 5' and 3' end mapping of the transcripts by S1 nuclease analysis. The results of these experiments indicated that the E-3 regions in these three viruses are shorter and simpler in organization than the E-3 regions of human adenoviruses. A potential promoter for the E-3 regions of these PAVs was identified.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
authors.forename: P S
authors.initials: PS
authors.lastname: Reddy
authors.forename: N
authors.initials: N
authors.lastname: Idamakanti
authors.forename: J B
authors.initials: JB
authors.lastname: Derbyshire
authors.forename: E
authors.initials: E
authors.lastname: Nagy
mesh_terms: D017503:Adenovirus E3 Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D017921:Mastadenovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013552:Swine
chemical_list: D017503:Adenovirus E3 Proteins; D004279:DNA, Viral; D015719:Single-Strand Specific DNA and RNA Endonucleases