title: Chromosome analysis by fluorescence in situ hybridization: further indications for a non-DNA-proportional involvement of single chromosomes in radiation-induced structural aberrations.
abstract: The frequencies of symmetrical complete and incomplete translocations and dicentrics induced in human lymphocytes after in vitro irradiation with 3Gy X-rays were analysed by the use of fluorescence in situ hybridization (FISH). Single whole chromosome painting (WCP) probes, specific for chromosomes 1-4, 6-10, 12, 14 and X were hybridized separately. A human pancentromeric DNA-probe was used simultaneously for unequivocal centromere detection. For both aberration types, symmetrical translocations and dicentrics, a significant deviation from a DNA-proportional distribution was found. In general, chromosomes with a higher DNA content (chromosomes 1-3, 6 and 7) were less frequently involved in the formation of symmetrical translocations and dicentrics than expected according to their DNA-content, whereas smaller chromosomes were more frequently involved. The only exception was chromosome 4, exhibiting the highest translocation frequency of all chromosomes analysed. Ratios of the yields of symmetrical translocations to the yields of dicentrics varied between 0.9 and 1.8 for the single chromosomes. The present results substantiate our previous data obtained with identical chromosomes but examined in four different triple combinations.
authors.affiliation: GSF-Forschungszenstrum für Umwelt und Gesundheit, Institut für Strahlenbiologie, Neuherberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Knehr
authors.forename: H
authors.initials: H
authors.lastname: Zitzelsberger
authors.forename: H
authors.initials: H
authors.lastname: Braselmann
authors.forename: U
authors.initials: U
authors.lastname: Nahrstedt
authors.forename: M
authors.initials: M
authors.lastname: Bauchinger
mesh_terms: D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects*; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D014178:Translocation, Genetic / Q000528:radiation effects
chemical_list: D004247:DNA