Racemization of lens crystalline constituents in UV-induced cataract evaluated by chiral GC/MS spectroscopy.
The racemization of amino acids is hypothesized to cause cataract by disrupting the crystalline's tertiary structure, which, in turn, alters the optical characteristics of the lens. To better understand the role of these modifications in cataractogenesis, the changes in stereoisomer ratio of amino acids from lens crystalline by UV-B-induced cataract in animal models were studied using chiral separation gas chromatography-single quadrupole mass spectroscopy. The anticataract action of a compound, ([1-(phenylmethyl)-1H-indazol-3-yl]oxy)acetic acid lysinate, was also evaluated by this method.
Jung
W T
WT
Department of Instrumental Analysis, Il Yang Central Research Institute, Kyungki, Korea.
Rho
S H
SH
Park
W C
WC
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002386:Cataract / Q000209:etiology* / Q000517:prevention & control; D003459:Crystallins / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007191:Indazoles / Q000494:pharmacology; D007908:Lens, Crystalline / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D011833:Radiation Injuries, Experimental / Q000209:etiology* / Q000517:prevention & control; D051381:Rats; D013237:Stereoisomerism; D013256:Steroids; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000596:Amino Acids; D000893:Anti-Inflammatory Agents; D003459:Crystallins; D007191:Indazoles; D013256:Steroids; C036067:bendazac lysine