Comparison of 2 centrifugation procedures in the formalin-ethyl acetate stool concentration technique for the detection of Cryptosporidium oocysts.
A comparison between the standard formalin-ethyl acetate (FEA) stool concentration procedure (centrifugation at 400 g for 2 min) and a modification proposed to increase the recovery of Cryptosporidium oocysts (500 g for 10 min) was performed on 73 formalin-preserved stool specimens known to be positive for Cryptosporidium. False-negative results were obtained with both methods, but the increase in centrifugation time and force resulted in the detection of a higher number of oocysts and positive samples. The modified procedure had a sensitivity significantly higher than the standard method (99% compared to 86%, P = 0.0045).
Clavel
A
A
Servicio de Microbiología y Parasitología, Hospital Clínico Universitario, Zaragoza, Spain.
Arnal
A
A
Sánchez
E
E
Varea
M
M
Quílez
J
J
Ramírez
I
I
Castillo
F J
FJ
D000085:Acetates; D000818:Animals; D002498:Centrifugation; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005557:Formaldehyde; D006801:Humans; D010274:Parasitology / Q000379:methods*; D015053:Zygote
D000085:Acetates; D005557:Formaldehyde; C007650:ethyl acetate