Induction of cytochrome P-450-linked monooxygenase system in rat liver microsomes by xiao-chaihu-tang.
The administration of Xiao-Chaihu-Tang (TJ-9) for 2 weeks induced a 25% increase in the content of cytochrome P-450 in female rat liver microsomes, while the content in male rats remained unchanged. The enzymatic activities toward various xenobiotics were stimulated in female rats, the levels being in the range of 125-250% of those in the control rats. Among these xenobiotics, the metabolic rates for substrates of cytochrome P-450 2E1 were significantly enhanced in female rats. On the other hand, the activities toward various xenobiotics in male rats were unchanged. When 3-methylcholanthrene was given to rats for a week, the augmentation of cytochrome P-450 content and the stimulation of xenobiotic metabolism were observed. However, co-administration of TJ-9 did not alter the effect of 3-methylcholanthrene described above.
Obnishi
T
T
Department of Biochemistry, Kagawa Medical School, Japan.
Yoneyama
H
H
Hamamoto
T
T
Ishida
T
T
Takahara
J
J
Ichikawa
Y
Y
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008297:Male; D008748:Methylcholanthrene / Q000008:administration & dosage / Q000633:toxicity; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D013379:Substrate Specificity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000970:Antineoplastic Agents; D002273:Carcinogens; D004365:Drugs, Chinese Herbal; D008748:Methylcholanthrene; C018142:saiko-keishi-to; D003577:Cytochrome P-450 Enzyme System; D019392:Cytochrome P-450 CYP2E1