Potentiation of GABAA receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin.
Application of Cerebrolysin (0.1 microgram per 1 ml) by a fast microperfusion system induced an inward current of 0.2 to 1 nA in all neurones from newborn mouse hippocampi held at -30 mV membrane potential. Cerebrolysin-induced currents were reduced by the GABAA antagonist bicuculline (2 microM) by 65%, by the NMDA antagonist aminophosphovaleric acid (APV, 10 microM) by 27% and by the non-NMDA antagonist cyanonitriquinoxalinedione (CNQX, 10 microM) by 20%. Cerebrolysin dialyzed through a 3.6 kD gut did not induce any transmembrane current but potentiated the response induced by GABA (10 microM) to 135%. We conclude that, in addition to amino acids which activate GABAA, NMDA and non-NMDA receptors, Cerebrolysin also contains a peptide which potentiates the GABAA receptor response.
Zemková
H
H
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Krůĕk
J
J
Vyskocil
F
F
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003956:Dialysis; D004594:Electrophysiology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D000596:Amino Acids; D018691:Excitatory Amino Acid Antagonists; D018756:GABA Antagonists; D011963:Receptors, GABA-A; C006952:cerebrolysin; D005680:gamma-Aminobutyric Acid; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D001640:Bicuculline