Spontaneous and mitomycin-C-induced micronuclei in lymphocytes from subjects affected by Turner's syndrome.
Peripheral blood lymphocytes from 15 subjects affected by Turner's syndrome (TS) and aged between 2 and 24 years (mean age 10.40 +/- 6.25) were tested to evaluate the spontaneous and Mitomycin-C-induced (MMC) micronucleus (MN) frequency. A group of 15 healthy subjects, in the same range of age (mean age 14.67 +/- 8.30), was also tested as control. As expected, statistically significant differences between spontaneous and MMC-induced MN were found either in TS and in healthy subjects. Unexpectedly, when the two groups of donors were compared, TS subjects showed a lower spontaneous and MMC-induced MN frequency, in comparison with healthy subjects. Cell proliferation kinetic and cytotoxicity were also measured applying the cytokinesis-block proliferation index (CBPI): the results show that MMC, at the employed concentration, does not induce cell cycle delay both in healthy and in TS donors. Whereas, when CBPI from TS and healthy donors were compared, a faster proliferation was found in TS patients in both untreated and MMC-treated cultures.
Scarfí
M R
MR
CNR-IRECE, Napoli, Italy.
Prisco
F
F
Bersani
F
F
Lioi
M B
MB
Zeni
O
O
Di Pietro
R
R
Franceschi
C
C
Motta
M
M
Iafusco
D
D
Stoppoloni
G
G
D000293:Adolescent; D000328:Adult; D000375:Aging; D002455:Cell Division / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D015162:Micronucleus Tests; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D014424:Turner Syndrome / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology
D009153:Mutagens; D016685:Mitomycin