title: In vitro attenuation of highly virulent infectious bursal disease virus: some characteristics of attenuated strains.
abstract: Some strains of highly virulent infectious bursal disease virus (HV-IBDV) were adapted through serial passage in embryonated eggs. The embryonated egg-adapted HV-IBDV was successfully adapted to grow in chicken embryo fibroblast (CEF) cell cultures showing a cytopathic effect by preparing the CEF cells from the virus-infected embryos. The embryonated egg- and cell culture-adapted strains significantly reduced their pathogenicity to, and did not kill any, young chickens in experimental infection. The bursal lesions of the adapted strain-infected chickens were similar to those observed in classical strain-infected chickens. Cross-virus neutralization analysis showed antigenic diversity between the cell culture-adapted HV-IBDV strains and classical strains. In immunization tests, the adapted strain-immunized chickens showed good protection against the fatal infection of HV-IBDV. Especially, in case of challenge at 3 days postimmunization, the adapted strains showed effective immunogenicity. The adapted strains appear to provide a new and effective live vaccine against HV-IBDV infection.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Inoshima
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: M
authors.initials: M
authors.lastname: Miyoshi
authors.forename: T
authors.initials: T
authors.lastname: Yanai
authors.forename: T
authors.initials: T
authors.lastname: Masegi
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary; D002642:Chick Embryo / Q000821:virology; D002645:Chickens / Q000821:virology; D007243:Infectious bursal disease virus / Q000276:immunology / Q000502:physiology*; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000517:prevention & control / Q000821:virology; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines