title: Dopamine inhibits in vitro release of VIP and proliferation of VIP-immunoreactive pituitary cells.
abstract: A double immunohistochemical study for VIP and proliferating cell nuclear antigen (PCNA) was carried out on monolayer cultures from adult male rats pituitary glands treated with dopamine (ranging from 10(-9) to 10(-5) M), in order to establish whether or not dopamine is involved in the regulation of the proliferation rate of pituitary VIP-immunoreactive cells. For all doses of dopamine assayed, the release of VIP to the culture medium, the numerical density of VIP-immunoreactive cells and the percentages of VIP- and PCNA-immunoreactive cells decreased significantly after dopamine treatment. These results suggest that dopamine could be a physiological inhibitor involved in the modulation of pituitary VIP proliferation rate.
authors.affiliation: Department of Human Anatomy and Histology, Universidad de Salamanca, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Carretero
authors.forename: R J
authors.initials: RJ
authors.lastname: Vazquez
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: L
authors.initials: L
authors.lastname: Cacicedo
authors.forename: M
authors.initials: M
authors.lastname: Rubio
authors.forename: F
authors.initials: F
authors.lastname: Sanchez-Franco
authors.forename: R
authors.initials: R
authors.lastname: Vazquez
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004298:Dopamine / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D014660:Vasoactive Intestinal Peptide; D004298:Dopamine