Immunodominant Entamoeba histolytica antigens recognized by serum and intestinal antibodies after local or systemic immunization of mice with glutaraldehyde fixed trophozoites.
We performed an immunoblot analysis of the main E. histolytica proteins recognized by immune sera and intestinal fluids of Balb/c mice immunized with glutaraldehyde fixed trophozoites (GFT) by intragastric, rectal and intraperitoneal routes, to determine if there were differences in the amebic antigens immunodominantly recognized at mucosal and systemic levels. The antigen patterns recognized by mice immunized via intraperitoneal and rectal routes were complex and similar suggesting that the immunization route (systemic or local), does not influence the recognition pattern elicited at mucosal or systemic levels. However, the number of amebic bands recognized after intragastric immunization was very low. The molecular weights of the principal amebic proteins recognized by serum antibodies were 150-130, 116, 104, 84, 56, 42, 18, and 16 kDa. The intestinal fluids of mice immunized via intraperitoneal and rectal routes contained antibodies that recognized five bands of 220-200, 150-134, 93-84, 43-41, and 16-14 kDa. These results suggest that there are differences in the number of immunodominant amebic antigens recognized at mucosal and systemic levels. Moreover we found that the bands of 150, 39 and 19 kDa. were mainly recognized by IgG, whereas the bands of 116, 93, and 16 were mainly recognized by IgM, indicating differences between the antigens immunodominantly recognized by serum antibodies from different isotypes.
Moreno-Fierros
L
L
Unidad de Morfología y Functión, ENEP-Iztacala, Universidad Nacional Autónoma de México, Estado de México, México, D.F., México.
Domínguez-Robles
M C
MC
Enríquez-Rincón
F
F
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D005976:Glutaral; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology
D000906:Antibodies; D016056:Immunodominant Epitopes; D015800:Protozoan Proteins; D005976:Glutaral