Studies on the viability of the isolated vascularly perfused rat colon.
The colon contains large numbers of endocrine cells. Insight into their physiological function is limited. This is due to the fact that no sufficient model of isolated endocrine colon cells is available. In the present study we introduce an isolated vascularly perfused colon model for in vitro studies. This model offers the advantage that it keeps the endocrine cells in their physiological orientation and environment. The gut mucosa is highly sensitive to ischemia. Therefore, a careful validation of its viability is crucial in gut organ preparations. This study demonstrates that, by utilizing an oxygenated vascular medium supplemented with 25% washed bovine erythrocytes, a perfusion of the colon is achieved for at least 1 h without obvious tissue injuries. During this time parameters such as perfusion pressure, venous lactate dehydrogenase release, glucose consumption, lactate output, oxygen consumption, perfusate loss by the preparation and morphology were analyzed. Dependent on stimulation, the endocrine L cells of the colon released glucagon-like peptide-I upon arterial perfusion of methacholine or gastrin-releasing peptide. In conclusion, a model for the isolated perfusion of the colon is introduced which is suitable for studies of endocrine colon cells.
Herrmann-Rinke
C
C
Department of Internal Medicine, Philipps University of Marburg, Germany.
Eissele
R
R
Arnold
R
R
Göke
B
B
D000818:Animals; D002470:Cell Survival; D003106:Colon / Q000098:blood supply* / Q000166:cytology; D005934:Glucagon / Q000378:metabolism; D052216:Glucagon-Like Peptide 1; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000166:cytology; D007739:L Cells / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000378:metabolism; D008297:Male; D051379:Mice; D010101:Oxygen Consumption; D010446:Peptide Fragments / Q000378:metabolism; D010477:Perfusion; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D007773:Lactates; D010446:Peptide Fragments; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D007770:L-Lactate Dehydrogenase; D005947:Glucose