title: Inhibition of the multiplication of Listeria monocytogenes in a murine hepatocyte cell line (ATCC TIB73) by IFN-gamma and TNF-alpha.
abstract: The effects of IFN-gamma and TNF-alpha on intracellular multiplication of Listeria monocytogenes in a murine enbryonic hepatocyte cell line (ATCC TIB 73) were investigated. Neither IFN-gamma nor TNF-alpha alone (10, 25, or 100 ng/ml) significantly inhibited intracellular multiplication of L. monocytogenes. In contrast, addition of both IFN-gamma and TNF-alpha (10, 25, or 100 ng/ml) significantly inhibited intracellular listerial multiplication. The anti-listerial effects of IFN-gamma and TNF-alpha were not blocked by adding aminoguanidine or catalase. Nor did IFN-gamma and TNF-alpha treated hepatocyte monolayers produce detectable amounts of nitric oxide or hydrogen peroxide. These findings suggest that the effects of IFN-gamma and TNF-alpha may be independent of reactive oxygen (ROI) and nitrogen (RNI) intermediates.
authors.affiliation: Department of Bacteriology, College of Agriculture and Life Sciences, University of Wisconsin-Madison, USA.
authors.forename: R
authors.initials: R
authors.lastname: Haponsaph
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D002460:Cell Line; D007371:Interferon-gamma / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008088:Listeriosis; D008099:Liver / Q000382:microbiology*; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma