Isolation and characterization of microvascular endothelial cells from chicken fat pads.
Microvascular endothelial cells from abdominal fat pads of 6-wk-old broiler chickens were isolated to provide an in vitro system to study their physiological functions. The isolation procedure produced clumps of 10-30 cells, which attached to culture vessels in 4 h and attained confluency in 2 wk. At confluency, cells had a cobblestone appearance but were not contact inhibited and detached from the bottom of the culture vessel 2 wk after reaching confluency. The cells internalized acetylated low density lipoproteins, a characteristic of endothelial cells. This property was used to obtain pure endothelial cell cultures using the cell sorter. When cultured over Matrigel, a reconstituted matrix, the cells aligned themselves into chordlike structures and formed branching microvessels. Cells plated on type I collagen-coated culture flasks occasionally formed chordlike structures and proliferated at a faster rate than cells plated on Matrigel. Cells cultured on laminin-coated plates were slender and had long cytoplasmic extensions; however, cells cultured on uncoated plastic had fibroblastic morphology. These properties are similar to those described for microvessel endothelial cells isolated from tissues of other species.
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Pennsylvania State University, Department of Poultry Science, University Park 16802, USA.
Leach
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D016475:3T3 Cells; D000818:Animals; D002232:Carbocyanines / Q000493:pharmacokinetics; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D005223:Fats; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats
D002232:Carbocyanines; D004338:Drug Combinations; D005223:Fats; D005456:Fluorescent Dyes; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; C072024:carbocyanine dye DiIC12(3); D003094:Collagen