Ca2+ and Cl(-)-dependence of the turtle olfactory response to odorants and forskolin.
In the turtle olfactory system, large responses to odorants appeared after application of cAMP of forskolin at high concentrations to the isolated olfactory receptor neurons or the olfactory epithelium, suggesting that a cAMP-independent pathway greatly contributes to the generation of odor responses. We measured the effects of the mucosal Ca2+ and Cl- concentrations and a Ca(2+)-activated Cl(-)-channel blocker, 4-acetamide-4'-(isothiocyano) stilbene-2,2'-disulphonic acid (SITS), upon olfactory bulbar responses to explore the contribution of the Ca(2+)-activated Cl(-)-channels to cAMP-dependent and independent pathways. Elimination of mucosal Ca2+ by addition of 2 mM EGTA to the stimulating solution partially inhibited the cAMP-independent responses to 0.1 mM citralva but did not affect those to 0.1 mM lilial or 0.1 mM l-carvone. Substitution of mucosal Cl- with gluconate slightly enhanced the total response to 0.1 mM citralva but practically did not affect the responses to other odorants tested. SITS (2 mM) partially inhibited the responses to 50 microM forskolin and 0.1 mM citralva but did not affect the cAMP-independent response to 0.1 mM citralva in Ca(2+)-free Ringer's solution. These results suggested that the Ca(2+)-activated Cl- channels do not contribute to the generation of the cAMP-independent responses, but that they partially contribute to the generation of the cAMP-dependent responses in the turtle.
Kashiwayanagi
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. yanagi@ph.hines.hokudai.ac.jp
Kawahara
H
H
Kanaki
K
K
Nagasawa
F
F
Kurihara
K
K
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology; D009812:Odorants; D009830:Olfactory Bulb / Q000187:drug effects / Q000502:physiology; D009831:Olfactory Mucosa / Q000166:cytology / Q000187:drug effects; D009833:Olfactory Pathways / Q000187:drug effects / Q000502:physiology; D012903:Smell / Q000187:drug effects / Q000502:physiology*; D013268:Stimulation, Chemical; D014426:Turtles / Q000502:physiology*
D018118:Chloride Channels; D005576:Colforsin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D000242:Cyclic AMP; D002118:Calcium