title: Repetitive hypoxic exposure of brain slices and electrophysiological responses as an experimental model for investigation of cerebroprotective measurements.
abstract: An in vitro hippocampal (CA 1 region, guinea pig) slice technique using repeated hypoxia was employed to model electrophysiological changes (DC-potentials and evoked potentials (EP) by stimulation of Schaffer-collaterals) occurring in the hypoxic CA1 pyramidal layer. A standardized neuronal response under repeated hypoxic conditions was observed in this model, consisting of disappearance of EP and a trend towards partially reversible, but progressive synaptic failure subsequent anoxic depolarisation (AD). Slices treated with the calcium antagonist nimodipine showed a prolongation of AD latency between the first and following hypoxias. So it seems possible to simulate hypoxic lesions of the brain tissue by using this in vitro slice model.
authors.affiliation: Klinik and Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Wassmann
authors.forename: D
authors.initials: D
authors.lastname: Moskopp
authors.forename: B
authors.initials: B
authors.lastname: Woesler
authors.forename: A
authors.initials: A
authors.lastname: Lücke
authors.forename: E J
authors.initials: EJ
authors.lastname: Speckmann
authors.forename: R
authors.initials: R
authors.lastname: Köhling
authors.forename: H
authors.initials: H
authors.lastname: Straub
authors.forename: S
authors.initials: S
authors.lastname: Hülsmann
authors.forename: C
authors.initials: C
authors.lastname: Greiner
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000503:physiopathology; D002121:Calcium Channel Blockers / Q000494:pharmacology / Q000627:therapeutic use; D015687:Cell Hypoxia; D004558:Electric Stimulation; D004734:Energy Metabolism; D005071:Evoked Potentials / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology*; D002534:Hypoxia, Brain / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology*; D008564:Membrane Potentials / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D009553:Nimodipine / Q000494:pharmacology / Q000627:therapeutic use; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission
chemical_list: D002121:Calcium Channel Blockers; D018696:Neuroprotective Agents; D009553:Nimodipine