Antibody responses against the G and F proteins of bovine respiratory syncytial virus after experimental and natural infections.
Antibodies against the two major surface glycoproteins of bovine respiratory syncytial virus (BRSV), G and F, play a role in protection against BRSV-associated disease, but only the antibody response against the F protein has been well described. Therefore, we used a novel peptide-based enzyme-linked immunosorbent assay (G peptide-ELISA) to compare immunoglobulin G (IgG) and IgG subclass antibody responses against the G protein with the antibody response against the F protein, as measured by a conventional BRSV ELISA (F-ELISA). Experimental infection of cattle induced significantly lower antibody titers than did natural infection. After natural primary infection, G peptide-specific antibodies declined more rapidly and to lower levels than the F protein-specific antibodies. As a consequence, the G peptide-ELISA detected more reinfections than did the F-ELISA. Ratios of G- and F-specific IgG1/IgG2 antibody titers did not differ markedly after infection or vaccination. Interestingly, after natural infection calves did not develop an IgG2 response to the complete G protein. In contrast, adult cattle had high IgG2 titers against this protein. Vaccination with a live vaccine induced low antibody titers, similar to the titers after experimental infection, whereas vaccination with an inactivated vaccine induced high titers. The results indicate that the kinetics of the G- and F-specific antibody responses differ. Furthermore, the IgG subclass response against the unglycosylated central region of the G protein is similar to the IgG subclass response to the F protein, but the IgG subclass response differs from the response to the complete G protein.
Schrijver
R S
RS
Department of Bovine Virology, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
Langedijk
J P
JP
van der Poel
W H
WH
Middel
W G
WG
Kramps
J A
JA
van Oirschot
J T
JT
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D015707:HN Protein; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D018357:Respiratory Syncytial Virus Infections / Q000209:etiology / Q000276:immunology* / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D015707:HN Protein; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G