Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.
Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.
Stockelberg
D
D
Department of Medicine, Sahlgrenska University Hospital, University of Göteborg, Sweden.
Hou
M
M
Rydberg
L
L
Kutti
J
J
Wadenvik
H
H
D000017:ABO Blood-Group System / Q000032:analysis*; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016824:Antigens, Human Platelet / Q000737:chemistry* / Q000276:immunology; D001792:Blood Platelets / Q000737:chemistry*; D018955:CD36 Antigens / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006031:Glycosylation; D006801:Humans; D007231:Infant, Newborn; D039661:Integrin beta3; D051379:Mice; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000737:chemistry* / Q000276:immunology; D011499:Protein Processing, Post-Translational; D013921:Thrombocytopenia / Q000151:congenital / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D018955:CD36 Antigens; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019038:Platelet Glycoprotein GPIb-IX Complex