Effect of cisplatin on the negative charge barrier in strial vessels of the guinea pig. A transmission electron microscopic study using polyethyleneimine molecules.
The anionic sites on the basement membrane in the cochlea are believed to act as a charge barrier. Using polyethyleneimine (PEI) as a cationic tracer, we examined the effects of cisplatin (CDDP) on anionic sites in the basement membrane in the cochlea. Eight Hartley-strain guinea pigs were separated into control (buffer administration) and CDDP groups. Ultrathin sections of the basal and third turns of the cochlear ducts were examined using a transmission electron microscope. A marked decrease of PEI distribution was noted in the strial vessels of the CDDP group compared to the control group. However, the two groups showed no significant differences in PEI particles on the basement membranes in the basilar and Reissner's membranes, or in the basal and third turns. These findings suggest that the charge barrier in the stria vascularis may be easily injured by the administration of CDDP.
Suzuki
M
M
Department of Otolaryngology, Tohsei National Hospital, Shizuoka, Japan.
Kaga
K
K
D000818:Animals; D000838:Anions; D001485:Basement Membrane / Q000098:blood supply / Q000648:ultrastructure; D001808:Blood Vessels / Q000187:drug effects* / Q000648:ultrastructure; D002945:Cisplatin / Q000009:adverse effects / Q000494:pharmacology*; D003051:Cochlea / Q000098:blood supply* / Q000187:drug effects; D006168:Guinea Pigs; D008833:Microcirculation / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D011094:Polyethyleneimine
D000838:Anions; D011094:Polyethyleneimine; D002945:Cisplatin