title: Polymerase chain reaction and other amplification techniques in mycobacteriology.
abstract: Amplification methods for detection of Mycobacterium tuberculosis, such as polymerase chain reaction (PCR), have undergone much research and development in the last several years. The most common methods for extraction, amplification, and detection of mycobacterial nucleic acid sequences used in "in-house" PCR assays are discussed. A list of commercially prepared PCR and non-PCR amplification assays that should be available soon is included. The pros and cons of "in-house" versus commercial technology and issues of implementation of molecular technology in the clinical laboratory are reviewed.
authors.affiliation: Clinical Microbiology, Laboratory, H. Lee Moffitt Cancer Center, Tampa, Florida, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Sandin
mesh_terms: D001431:Bacteriological Techniques; D001432:Bacteriology; D007753:Laboratories; D009161:Mycobacterium / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015347:RNA Probes; D012680:Sensitivity and Specificity
chemical_list: D015347:RNA Probes