RNA molecules lighting up under the microscope.
RNA in situ hybridization is a useful method for localizing specific mRNAs and studying the spatial and temporal organization of RNA transcription, processing and transport in cells. In this review, I describe methods of RNA in situ hybridization for tissue sections and cell preparations. Special emphasis is placed on the application of non-radioactive-labeled probes for multiparameter cell analysis. In addition, a summary of RNA in situ hybridization studies on RNA transport in the cytoplasm as well as in the nucleus of cells is given.
Dirks
R W
RW
Department of Cytochemistry and Cytometry, Sylvius Laboratories, Leiden University, Netherlands.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary; D016591:Histocytological Preparation Techniques; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008853:Microscopy; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism
D018076:DNA, Complementary; D015347:RNA Probes; D012333:RNA, Messenger; D012313:RNA