Fluorescent labelling of pyruvate carboxylase with 4-acetamido-4'-(iodacetyl)-aminostilbene-2,2'-disulfonic acid (AIDA) provides a means of monitoring substrate binding.
Attwood
P V
PV
Department of Biochemistry, University of Western Australia, Nedlands, Australia.
Powell
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D011766:Pyruvate Carboxylase / Q000737:chemistry* / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013267:Stilbenes; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents
C090885:4-acetamido-4'-((iodoacetyl)amino)stilbene-2,2'-disulfonate; D005456:Fluorescent Dyes; D013267:Stilbenes; D013439:Sulfhydryl Reagents; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D011766:Pyruvate Carboxylase