title: Improved automated bone marrow processing and enrichment of CD34+ cells by a large-volume apheresis procedure.
abstract: We investigated the efficacy of bone marrow (bm) processing by a large-volume apheresis procedure using a self constructed sixfold collection bag system for sequential cell collection and analysis for 5 pediatric patients. Quantitation of leukocytes (WBC), CD34+ cells and colony-forming cells (CFU-GM) within the single bags showed a relative time-dependent decrease of all cell fractions during leukapheresis, whereas the relative amount of mononuclear cells (MNC) droped only slightly. At the same time the large volume apheresis (6 x original bm-volume) clearly enhanced the absolute yield of CD34+ cells compared to the standard procedure (3 x bm-volume) for more than 20%. We conclude that large-volume apheresis for bm processing is an efficient technique to improve the yields of progenitor cells.
authors.affiliation: Institut für Transfusionsmedizin, Westfälische Wilhelms-Universität Münster, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Cassens
authors.forename: H
authors.initials: H
authors.lastname: Garritsen
authors.forename: R
authors.initials: R
authors.lastname: Ernst
authors.forename: H
authors.initials: H
authors.lastname: Jürgens
authors.forename: W
authors.initials: W
authors.lastname: Sibrowski
mesh_terms: D000293:Adolescent; D018952:Antigens, CD34 / Q000032:analysis*; D001781:Blood Component Removal / Q000379:methods*; D016026:Bone Marrow Transplantation / Q000276:immunology; D001859:Bone Neoplasms / Q000628:therapy; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D012208:Rhabdomyosarcoma / Q000628:therapy; D012512:Sarcoma, Ewing / Q000628:therapy; D012983:Soft Tissue Neoplasms / Q000628:therapy
chemical_list: D018952:Antigens, CD34