Aging and polyamine acetylation in rat kidney.
The acetylation of polyamines was investigated in rat kidney as a function of age. The activity of cytosolic spermidine/spermine N1-acetyltransferase, the rate-limiting enzyme in polyamine interconversion, increased from 3 to 36 months of age. The activity of cytosolic spermidine N8-acetyltransferase, an enzyme probably related to polyamine excretion, also increased. The activity of polyamine oxidase, which catalyzes the oxidative cleavage of polyamine N1-acetyl derivatives into putrescine, decreased until 24 months, when an accumulation of N1-acetylspermidine occurred. Subsequently, at 36 months, polyamine oxidase activity returned toward high values, in concomitance with the disappearance of N1-acetylspermidine, an increase in spermidine and putrescine, and a decline in spermine was observed. Our results show that in rat kidney during aging there is an activation of the acetylation and interconversion of higher polyamines into putrescine, which is considered an alternative pathway of spermidine and putrescine formation.
Ferioli
M E
ME
Centro di Studio sulla Patologia Cellulare, C.N.R., Università di Milano, Milan, Italy.
Sessa
A
A
Tunici
P
P
Pinotti
O
O
Perin
A
A
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D006657:Histones / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000031:analogs & derivatives / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013379:Substrate Specificity; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D006657:Histones; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D011700:Putrescine; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase; C017988:N(1)-acetylspermidine