ATP-diphosphophydrolase activity in rat heart tissue.
Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart sarcolemma, under conditions where mitochondrial ATPase and adenylate kinase were blocked, supports our proposal that both activities correspond to a single enzyme, known as ATP-diphosphohydrolase or apyrase. The physiological function of this enzyme could be dephosphorylation of the nucleotides present in the interstitial heart compartment acting together with 5'-nucleotidase. Both hydrolytic activities have similarities in: sarcolemma localization, bivalent metal ion dependence, optimum pH, effect of several amino acid residue modifiers, competitive inhibition of nucleotide analogs, and broad nucleoside di-and triphosphate specificity. The ATPase activity could not be separated from the ADPase either through isoelectrofocusing or electrophoresis under acid conditions.
Espinosa
V
V
Departamento de Bíoquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
Galleguillos
M
M
Mancilla
M
M
Garrido
J
J
Kettlun
A M
AM
Collados
L
L
Chayet
L
L
García
L
L
Traverso-Cori
A
A
Valenzuela
M A
MA
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001081:Apyrase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D002412:Cations / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006321:Heart / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012508:Sarcolemma / Q000187:drug effects / Q000201:enzymology; D013379:Substrate Specificity
D000596:Amino Acids; D002412:Cations; D004791:Enzyme Inhibitors; D008670:Metals; D009840:Oligomycins; D001081:Apyrase