Morphological analysis of an isolated fraction containing secretory granules from the submandibular gland of Trypanosoma cruzi infected and normal rats.
Normal and Trypanosoma cruzi infected submandibular glands from 42 male Holtzman rats were homogenized in 340 mM sucrose and 0.5 mM EDTA in 10 mM HEPES buffer at pH 7.4. The extract was centrifuged and filtered to prepare a granule rich fraction. Isolated secretory granules are similar morphologically to granules observed in whole submandibular gland preparations. Our results suggest that our isolation technic can be used for morphological and biochemical studies.
Borges-Silva
G A
GA
Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Bento-Alves
J
J
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology / Q000473:pathology*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000648:ultrastructure*; D014349:Trypanosoma cruzi / Q000302:isolation & purification*