title: Effects of interleukin-1 beta on scanning electron microscopic appearance and thyroid peroxidase content of human thyrocytes in monolayer culture.
abstract: Interleukin (IL-1), an inflammatory cytokine that is detected in the thyroid tissues of patients with autoimmune thyroiditis, is believed to be involved in the disease process. To clarify the role of IL-1 in the development of autoimmune thyroiditis, we investigated the effects of interleukin-1 beta (IL-1 beta) on the morphology of human thyrocytes in monolayer culture as well as the effect on thyroid peroxidase (TPO) content of these cells. Human normal thyrocytes were cultured with IL-1 beta for 4 days in the presence and absence of TSH. In morphologic studies, cultured cells were fixed for examination by scanning electron microscopy and for immunofluorescent staining of acting filaments. IL-1 produced striking morphologic changes in the cultured thyrocytes, including the cytoplasmic retraction and dissociation and/or depolymerization of actin filaments. These changes were unrelated to TSH stimulation. For detection of TPO, cultured cells were stained by an immunofluorescent technique and analyzed by fluorescence photometry. IL-1 reduced the TPO content and inhibited the TSH-induced increase in TPO in a concentration-dependent manner. These morphological changes and the reduction in TPO content of cultured thyrocytes suggest that IL-1 modulates the pathophysiology of autoimmune thyroiditis.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Asakawa
authors.forename: J
authors.initials: J
authors.lastname: Miyagawa
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Hanafusa
authors.forename: M
authors.initials: M
authors.lastname: Kuwajima
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007453:Iodide Peroxidase / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D013961:Thyroid Gland / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D013972:Thyrotropin; D007453:Iodide Peroxidase