title: Ultrastructural studies on the replication and morphogenesis of Nairobi sheep disease virus, a Nairovirus.
abstract: The Nairovirus Nairobi sheep disease virus (NSDV) affects sheep and goats causing severe hemorrhagic gastroenteritis and high mortality. Replication and morphogenesis of NSDV was determined by electron microscopic examination of ultra-thin sections of 143B and BHK-21 cells at varying times after infection. By 4 h post-infection (p.i.) of 143B cells, virions budding from the luminal side of the bilayer membrane of smooth membrane vesicles were observed. Morphologically mature virus particles were electron-dense, spherical and of uniform size (100 nm diameter) and accumulated in smooth membrane vesicles associated with the Golgi complex. In BHK-21 clone 13 cells, mature virus particles in smooth membrane vesicles were present by 8 h p.i. The morphogenesis of NSDV was restricted to the smooth membrane vesicles of Golgi complex, and budding of virus from other sites was not detected. Extracellular virus particles were observed by 10 h p.i., before expression of cytopathic effects. The cytopathic effects were observed at 24 h p.i. in 143B cells and at 36 h p.i. in BHK-21 cells. The morphology and morphogenesis of NSDV in BHK-21 cells and in 143B cells resembles that of other members of the family Bunyaviridae.
authors.affiliation: Small Ruminant Collaborative Research Support Program (SR-CRSP), Kenya Agricultural Research Institute.
authors.forename: P M
authors.initials: PM
authors.lastname: Rwambo
authors.forename: M K
authors.initials: MK
authors.lastname: Shaw
authors.forename: F R
authors.initials: FR
authors.lastname: Rurangirwa
authors.forename: J C
authors.initials: JC
authors.lastname: DeMartini
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D009024:Morphogenesis; D016855:Nairovirus / Q000502:physiology* / Q000648:ultrastructure; D012756:Sheep; D014779:Virus Replication