The determination of the extracellular concentration of brain glutamate using quantitative microdialysis.
Quantitative microdialysis with two enzyme-based assays was used to determine the extracellular concentration of glutamate in the striatum of freely moving rats. From the difference between infused and dialysate glutamate a value of 3.0 +/- 0.6 microM for the extracellular glutamate concentration was computed by regression analysis. The in vivo recovery, derived from the slope of the regression line, was 50%.
Miele
M
M
University Laboratory of Physiology, Oxford, UK.
Berners
M
M
Boutelle
M G
MG
Kusakabe
H
H
Fillenz
M
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D018698:Glutamic Acid