Infrequent alterations of the APC and MCC genes in gastric cancers from British patients.
We examined 26 gastric carcinomas from British patients for mutations of the APC gene using a single-strand conformation polymorphism (SSCP) and heteroduplex assay in conjunction with the protein truncation test (PTT). In addition, we performed loss of heterozygosity (LOH) analysis of the APC and MCC genes. We detected an inactivating somatic mutation in one gastric tumour. LOH of APC was observed in one of 12 informative cases (8%) and of MCC in two of 20 cases (10%). We thus find that alteration of the APC and MCC genes are infrequent in gastric cancers from the British population. Tumour-suppressor genes on other chromosomes must play a more significant role in the development of these tumours.
Sud
R
R
Human Genetics Group, Galton Laboratory, University College London, UK.
Talbot
I C
IC
Delhanty
J D
JD
D025601:Adenomatous Polyposis Coli Protein; D000483:Alleles; D001483:Base Sequence; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000737:chemistry; D004739:England / Q000208:ethnology; D017353:Gene Deletion; D017491:Genes, APC / Q000235:genetics*; D017431:Genes, MCC / Q000235:genetics*; D006801:Humans; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017384:Sequence Deletion; D013274:Stomach Neoplasms / Q000737:chemistry / Q000208:ethnology / Q000235:genetics*
D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins