SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae.
The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.
Cliften
Paul
P
Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
Wang
Yeelan
Y
Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
Mochizuki
Daisuke
D
Department of Fermentation Technology, Hiroshima University, 1-4-1 Higashi-Hiroshima 739, Japan.
Miyakawa
Tokichi
T
Department of Fermentation Technology, Hiroshima University, 1-4-1 Higashi-Hiroshima 739, Japan.
Wangspa
Rungrach
R
Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
Hughes
Joanne
J
Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
Takemoto
Jon Y
JY
Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D009154:Mutation; D011549:Pseudomonas / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C032677:syringomycin; D006899:Mixed Function Oxygenases; C109313:SUR2 protein, S cerevisiae