title: Modulation of autotransplanted adrenal gland by endothelin-1: a morphological and biochemical study.
abstract: BACKGROUND
  Adrenal gland autotransplantation, a model of cortical tissue regeneration, provides the reconstruction of distinct functional and morphological zonae. A morphological and biochemical study of the adrenal gland of adult male rats after autotransplantation and endothelin-1 (ET-1) administration was made.
  
  
  METHODS
  The technique involved bilateral adrenalectomy and placement of pieces of the adrenal gland in a dorsal plane between the skin and muscle. The animals were killed 90 days after the autotransplantation and 1 hr after intravenous ET-1 administration (0.5 microgram/kg body weight). The autotransplanted pieces were removed, fixed, and processed for light and electron microscopic morphologic studies. Trunk blood was collected for steroid assay.
  
  
  RESULTS
  Saline-treated control autotransplanted animals showed no remarkable differences in adrenal organization; grafts exhibiting a mass of regenerated cortical tissue were arranged in nests of glandular cells surrounded by a fibrous capsule and intersected by layers of connective tissue. The adrenal medulla was systematically absent. Ultrastructure of ET-1-treated animals revealed an inner area in the graft, consisting mainly of fasciculatalike cells. Cytoplasmic changes were evident, with high variations in mitochondrial size and arrangement. Profiles of smooth endoplasmic reticulum sometimes exhibited evidence of hypertrophy. Glandular cells in the graft outer area (subcapsular) were almost invariably like glomerulosa; however, some of them showed mitochondria with a peculiar arrangement of the cristae. "Hybrid" cells with mitochondria resembling those of the zona reticularis were also observed in the subcapsular environment. ET-1-stimulated animals showed significant increases in plasma corticosterone and aldosterone concentrations.
  
  
  CONCLUSIONS
  Endothelin-1, previously reported to stimulate acutely the aldosterone secretion by the adrenal zona glomerulosa in the rat, seems to exert a modulator role on the physiology of adrenal autotransplants, their regeneration and secretion.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Oporto, Porto, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Vendeira
authors.forename: D
authors.initials: D
authors.lastname: Neves
authors.forename: M M
authors.initials: MM
authors.lastname: Magalhães
authors.forename: M C
authors.initials: MC
authors.lastname: Magalhães
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects* / Q000637:transplantation* / Q000648:ultrastructure; D000818:Animals; D019332:Endothelin-1 / Q000494:pharmacology*; D006728:Hormones / Q000097:blood; D007275:Injections, Intravenous; D008297:Male; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic
chemical_list: D019332:Endothelin-1; D006728:Hormones