High-speed analysis of catecholamines in plasma by medium-pressure mu-LC with an amperometric detector.
A high-speed highly sensitive and reproducible method for the determination of catecholamines in small plasma volumes has been developed. Catecholamines were separated on a short capillary fused-silica column (0.32 mm i.d.) packed with a reverse-phase sorbent with a particle size of 10 microns. The time of a single chromatographic analysis was less than 6 min, and the operating pressure in the system was not higher than 5.0 MPa. The required volume of blood plasma was 0.5 mL. When a 10 microL sample previously concentrated on alumina was injected into the column, the limits of amperometric detection for noradrenaline (NA), adrenaline (A), 3,4-dihydroxy-benzylamine (DHBA), and dopamine (DA) were 3.9, 2.6, 1.8 and 5.6 ng/L, respectively. The average variation coefficient was 17.4%.
Ruban
V F
VF
Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg, Russia.
Petunin
S N
SN
Merkulova
N C
NC
D002395:Catecholamines / Q000097:blood*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D015203:Reproducibility of Results; D018401:Sample Size; D012680:Sensitivity and Specificity
D002395:Catecholamines