Lung epithelial cell (A549) interaction with unopsonized environmental particulates: quantitation of particle-specific binding and IL-8 production.
The A549 cell line was used to model in vitro the interaction of alveolar epithelium with environmental particulates. Confocal and electron microscopy demonstrated A549 binding and internalization of titanium dioxide (TiO2), iron oxide (Fe2O3), concentrated ambient air particulates (CAPs), and the fibrogenic particle alpha-quartz. Flow cytometry allowed quantitation of particle binding by measuring increased right angle light scatter (RAS) (TiO2) [40 micrograms/mL], Fe2O3 [100 micrograms/mL], alpha-quartz [200 micrograms/mL], or CAPs [40 micrograms/mL] fold increase RAS: 8.1 +/- 0.9, 4.3 +/- 0.4, 2 +/- 0.1, 1.6 +/- 0.1, respectively). With this quantitative assay, binding of particle was found to be calcium-dependent for TiO2 and Fe2O3 (% inhibition, 61.0 +/- 1.9, 40.0 +/- 5.6, respectively), while alpha-quartz binding was calcium-independent. A panel of polyanionic ligands known to inhibit scavenger-type receptors was used to identify binding mechanisms for environmental particulates. Both heparin and polyinosinic acid (polyI), but not the control polyanion chondroitin sulfate, caused marked inhibition of particulate binding by A549 cells (e.g., TiO2 [40 micrograms/mL] binding; polyI, heparin, and chondroitin sulfate: 73.8 +/- 3.5, 75.5 +/- 6.0, 7.5 +/- 6.7% inhibition, respectively; mean +/- SE, n > or = 4), indicating that scavenger receptor(s), albeit those distinct from the heparin-insensitive acetylated-LDL receptor, mediate particulate binding. The particulates ability to stimulate interleukin (IL-8) production in A549 cells was also tested. alpha-quartz, but not TiO2 or CAPs, caused a dose-dependent production of IL-8 (range 1-6 ng/mL), demonstrating a particle-specific spectrum of epithelial cell cytokine (IL-8) response. The results suggest that lung epithelial cell interaction with environmental particles is mediated by distinct receptors and can lead to particle dependent cytokine responses.
Stringer
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Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Imrich
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A
Kobzik
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D000393:Air Pollutants / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000502:physiology; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005290:Ferric Compounds / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D008565:Membrane Proteins; D009895:Opsonin Proteins / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011791:Quartz / Q000378:metabolism; D011971:Receptors, Immunologic / Q000502:physiology; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014025:Titanium / Q000378:metabolism
D000393:Air Pollutants; D005290:Ferric Compounds; D016209:Interleukin-8; D008565:Membrane Proteins; D009895:Opsonin Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D011791:Quartz; C009495:titanium dioxide; C000499:ferric oxide; D014025:Titanium; D002118:Calcium