title: The proliferation of human T lymphocytes stimulated by Helicobacter pylori antigens.
abstract: Fractionated mononuclear cells (MNCs) were obtained from peripheral blood of healthy human volunteers, seronegative for H. pylori antibodies. The MNCs were stimulated in culture with whole live or heat-killed H. pylori cells or with bacterial cell surface (SA) or cytoplasmic (CA) antigens. There was a marked proliferative response of T cells in cultures stimulated with 10(5) cells/well of live H. pylori, 5 micrograms/well of CA or 5-20 micrograms/well of SA. However, no proliferation was observed in MNC cultures containing higher "doses" of live H. pylori organisms (10(7)/well) or CA (20 micrograms/well). Moreover, higher "doses" of the bacteria or CA entirely inhibited the response of T cells to PHA.
authors.affiliation: Department of Medical Microbiology, Lund University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Chmiela
authors.forename: B
authors.initials: B
authors.lastname: Paziak-Domanska
authors.forename: A
authors.initials: A
authors.lastname: Ljungh
authors.forename: T
authors.initials: T
authors.lastname: Wadström
authors.forename: W
authors.initials: W
authors.lastname: Rudnicka
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000494:pharmacology*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000276:immunology; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial