Serological analysis of canine distemper virus using an immunocapture ELISA.
As a rapid and sensitive method to detect canine distemper virus (CDV), an immunocapture enzyme-linked immunosorbent assay (ELISA) was performed. The sensitivity and specificity of the immunocapture ELISA were considered to be high enough. Virus neutralizing (VN) test was also established using the immunocapture ELISA. By using this test, the different cross VN titers between sera of dogs experimentally infected with the Onderstepoort strain and those with a field isolate of CDV were observed.
Gemma
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Iwatsuki
K
K
Shin
Y S
YS
Yoshida
E
E
Kai
C
C
Mikami
T
T
D000818:Animals; D004216:Distemper / Q000175:diagnosis*; D004217:Distemper Virus, Canine / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009500:Neutralization Tests; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013045:Species Specificity