title: Intracellular pH change does not accompany egg activation in the mouse.
abstract: In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean pH, was 6.96 +/- 0.004 (+/- SEM) in GV-intact oocytes, 7.00 +/- 0.01 in ovulated, unfertilized eggs, and 7.02 +/- 0.01 in fertilized zygotes, indicating no sustained changes in pHi after germinal vesicle breakdown (GVBD) or fertilization. To examine whether transient changes in pHi occur shortly after egg activation, mouse eggs were parthenogenetically activated by 7% ethanol in phosphate buffered saline (PBS); no significant change in pHi followed ethanol activation. Since increased Na+/H+ antiporter activity is responsible for pHi increase in the sea urchin, pHi was measured in the absence of added bicarbonate or CO2 (a condition under which the antiporter would be the only major pHi regulatory mechanism able to operate, since the others were bicarbonate-dependent) in GV-intact oocytes, ovulated eggs, and in vivo fertilized zygotes to determine whether a Na+/H+ antiporter was activated. There was no physiologically significant difference in pHi after GVBD or fertilization, when pHi was measured in bicarbonate-free medium, nor any change upon parthenogenetic activation. Thus, a change in pHi is not a feature of egg activation in the mouse.
authors.affiliation: Loeb Medical Research Institute, Ottawa Civic Hospital, Ontario, Canada.
authors.forename: K P
authors.initials: KP
authors.lastname: Phillips
authors.forename: J M
authors.initials: JM
authors.lastname: Baltz
mesh_terms: D000818:Animals; D001578:Benzopyrans; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D005306:Fertilization / Q000502:physiology; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008815:Mice, Inbred Strains; D009284:Naphthols / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010312:Parthenogenesis / Q000502:physiology; D012235:Rhodamines / Q000378:metabolism; D015053:Zygote / Q000378:metabolism*
chemical_list: D001578:Benzopyrans; D003470:Culture Media; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009284:Naphthols; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D000431:Ethanol; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein