title: Platelet adhesion to novel phospholipid materials: modified phosphatidylcholine covalently immobilized to silica, polypropylene, and PTFE materials.
abstract: Based on the premise of achieving blood compatibility through mimicking the chemical constitutents of the biologically insert surface of the unactivated platelet membrane, a process was developed that entails the covalent grafting of modified phosphatidylcholine molecules to materials including silica, polypropylene, and polytetrafluoroethylene (PTFE) polymer films. These materials were characterized using x-ray photoelectron spectroscopy (XPS) and contactangle measurements. The phosphatidylcholine-containing materials (PC materials) were used as substrates in the plateletadhesion assays and were subjected to enzymatic degradation evaluation. Phosphatidylcholine-grafted silica materials do not support platelet adhesion. In addition the number of adherent platelets correlate with the amount of grafted phospholipid present, as indicated by the phosphorus/ carbon ratio obtained by XPS analysis. Platelet adhesion to phosphatidylcholine-grafted polypropylene and PTFE was inhibited 80% and 90%, respectively, when compared with platelet adhesion to unmodified polypropylene and PTFE.
authors.affiliation: Avecor Cardiovascular, Inc. Plymouth, Minnesota 55441, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Köhler
authors.forename: P J
authors.initials: PJ
authors.lastname: Parks
authors.forename: D L
authors.initials: DL
authors.lastname: Mooradian
authors.forename: G H
authors.initials: GH
authors.lastname: Rao
authors.forename: L T
authors.initials: LT
authors.lastname: Furcht
mesh_terms: D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D010713:Phosphatidylcholines; D010973:Platelet Adhesiveness; D011126:Polypropylenes; D011138:Polytetrafluoroethylene; D012822:Silicon Dioxide; D013052:Spectrometry, X-Ray Emission; D010738:Type C Phospholipases
chemical_list: D001672:Biocompatible Materials; D010713:Phosphatidylcholines; D011126:Polypropylenes; D012822:Silicon Dioxide; D011138:Polytetrafluoroethylene; D010738:Type C Phospholipases