title: Effect of antibodies to staphylococcal alpha and beta toxins and Staphylococcus aureus on the cytotoxicity for and adherence of the organism to bovine mammary epithelial cells.
abstract: OBJECTIVE
  To determine the effect of antibodies to staphylococcal alpha and beta toxins and Staphylococcus aureus on the toxicity for and adherence of S aureus to bovine mammary epithelial cells.
  
  
  METHODS
  Cultured bovine mammary epithelial cells and Staphylococcus aureus obtained from a cow with mastitis.
  
  
  METHODS
  Cultured bovine epithelial cells were incubated with antisera to alpha and beta toxins of S aureus and culture supernatant; cell damage and S aureus adherence to cells were measured.
  
  
  RESULTS
  Antisera to alpha, beta, and alpha + beta toxins inhibited cytotoxicity of S aureus culture supernatant. Antiserum to alpha + beta toxin was the most effective inhibitor of cytotoxicity and antiserum to beta toxin was the least effective. All 3 antisera decreased the percentage of S aureus adhered to the mammary epithelial cell monolayers and numbers of organisms per cluster of adhered bacteria. In this study, antisera to alpha and alpha + beta toxins decreased the number of S aureus clusters per dish, but antiserum to beta toxin had no significant effect. Antiserum to alpha + beta toxin decreased the percentage of epithelial cells with adhered S aureus, but neither antiserum to alpha nor beta toxin had significant effect. Antiserum to S aureus decreased the percentage of S aureus adhered, number of clusters perdish, number of organisms per cluster, and percentage of epithelial cells with S aureus adhered.
  
  
  CONCLUSIONS
  Antibodies to staphylococcal alpha and beta toxins inhibit adherence to and cytotoxicity of S aureus for bovine mammary epithelial cells, and antibodies to S aureus inhibit adherence of S aureus to bovine mammary epithelial cells.
authors.affiliation: Department of Animal Sciences, University of Maryland, College Park 20705, USA.
authors.forename: E
authors.initials: E
authors.lastname: Cifrian
authors.forename: A J
authors.initials: AJ
authors.lastname: Guidry
authors.forename: C N
authors.initials: CN
authors.lastname: O'Brien
authors.forename: W W
authors.initials: WW
authors.lastname: Marquardi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D006460:Hemolysin Proteins / Q000276:immunology*; D006461:Hemolysis; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000166:cytology; D011817:Rabbits; D013108:Sphingomyelin Phosphodiesterase; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D000906:Antibodies; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; D013108:Sphingomyelin Phosphodiesterase; C029878:hlb protein, Staphylococcus aureus