Occurrence of immunoreactive activin/inhibin beta(B) in gonadotrophs, thyrotrophs, and somatotrophs of the Xenopus pituitary.
An antibody against the Xenopus activin/inhibin beta(B) subunit (94-107) was raised in a rabbit. Using this antibody, the distribution of activin/inhibin beta(B) immunoreactivity in the pituitary of adult X. laevis was studied. Beta(B) immunoreactivity was detected in gonadotrophs, thyrotrophs, and somatotrophs under light microscopy. Electron microscopy revealed that a beta(B)-immunoreactive substance exists in LH, TSH, and GH granules, in contrast to findings in the rat and goldfish. These results indicate that the expression of activin/inhibin beta(B) in pituitary cells is not consistent among vertebrate species.
Uchiyama
H
H
Department of Biology, Faculty of Science, Yokohama City University, Yokohama, Japan.
Komazaki
S
S
Asashima
M
M
Kikuyama
S
S
D028341:Activins; D000818:Animals; D000918:Antibody Specificity; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D007265:Inhibins / Q000032:analysis*; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism*; D014982:Xenopus laevis / Q000502:physiology*
D006065:Gonadotropins, Pituitary; D028341:Activins; D007265:Inhibins; D013972:Thyrotropin; D013006:Growth Hormone