title: Breakpoint diversity illustrates distinct mechanisms for Robertsonian translocation formation.
abstract: Robertsonian translocations are the most common chromosomal rearrangements in humans. The vast majority of the ten possible nonhomologous types of Robertsonian translocations ascertained are rob(13q14q) and rob(14q21q). Recombination between homologous sequences on nonhomologous chromosomes has been proposed as a mechanism leading to the preferential formation of rob(13q14q) and rob(14q21q). However, little evidence exists to indicate whether the remaining less common Robertsonian translocations form through a similar mechanism. To better elucidate the mechanisms involved in Robertsonian translocation formation, we have used fluorescence in situ hybridization to localize the breakpoints in 56 nonhomologous Robertsonian translocations. This study revealed highly variable locations of breakpoints in seven types of the less common Robertsonians, while nearly all rob(13q14q) and rob(14q21q) analyzed displayed breakpoints in the same locations. Therefore, this study provides direct evidence that rob(13q14q) and rob(14q21q) form through a specific mechanism, possibly involving homologous recombination, which is distinct from the mechanism(s) that contributes to the formation of the remaining types of Robertsonian translocations.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Page
authors.forename: J C
authors.initials: JC
authors.lastname: Shin
authors.forename: J Y
authors.initials: JY
authors.lastname: Han
authors.forename: K H
authors.initials: KH
authors.lastname: Choo
authors.forename: L G
authors.initials: LG
authors.lastname: Shaffer
mesh_terms: D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite