False-positive serologic test resulting from a probable yeast infection in a chimpanzee.
Sera used to identify putative hepatitis E viral proteins expressed in Pischia pastoris produced a false-positive reaction because of antibodies to a yeast protein. This report illustrates a potential problem when serological reagents are used in combination with recombinant proteins expressed in yeast.
Heller
T
T
Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0740, USA. Theller@atlas.niaid.nih.gov
Yin
S
S
Tsarev
S A
SA
Purcell
R H
RH
Emerson
S U
SU
D000818:Animals; D005189:False Positive Reactions; D016751:Hepatitis E / Q000175:diagnosis; D016752:Hepatitis E virus / Q000276:immunology; D009181:Mycoses / Q000150:complications / Q000276:immunology*; D002679:Pan troglodytes; D010843:Pichia / Q000276:immunology* / Q000472:pathogenicity