title: Histochemical detection of age- and injury-related changes in signal transduction in the superior cervical ganglion.
abstract: The superior cervical ganglion (SCG) is thought to be a good model for correlation studies of morphology, function and metabolism of neurons. The SCG has a relatively simple organization, it can be easily manipulated in situ, and it maintains synaptic transmission and a high metabolic rate during in vitro incubations. The histology and structure of SCG neurons have been characterized in detail, and physiologic stimuli, injury and aging have all been found to induce changes in the SCG morphology. During the last decade, research in the field of signal transduction has greatly expanded. Several signal transduction pathways have been identified that participate in the regulation of neurotransmitter synthesis, gene expression, neuronal excitability and growth factor responses of sympathetic neurons. We have been interested in using the SCG to study some of the second and third messengers involved in converting external stimuli received by sympathetic neurons into cellular short- and long-term events. Using immunohistochemistry, we have investigated protein kinase C-subtypes and the immediate early gene product Fos in the SCG, and characterized some of the changes induced by injury and aging in these messenger molecules. We will review the results and discuss the advantages and disadvantages of using histological methods in the study of signal transduction in sympathetic neurons.
authors.affiliation: A.I. Virtanen Institute, Kuopio University Hospital, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Roivainen
authors.forename: J
authors.initials: J
authors.lastname: Koistinaho
mesh_terms: D000375:Aging; D000818:Animals; D005786:Gene Expression Regulation; D016762:Genes, fos; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009538:Nicotine / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D017783:Superior Cervical Ganglion / Q000293:injuries* / Q000503:physiopathology*
chemical_list: D007527:Isoenzymes; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D009538:Nicotine; D011493:Protein Kinase C