Photoimmobilization of insulin onto polystyrene dishes for protein-free cell culture.
Photoreactive insulin was synthesized by coupling with azidobenzoic acid. The insulin derivative was immobilized onto the wells of polystyrene culture plates by photoir-radiation. The photoimmobilized insulin enhanced the growth of anchorage-dependent cells such as Chinese hamster ovary CHO-K1 and mouse fibroblast STO cells by more than native or azidophenyl-derivatized insulin. A small amount of photoimmobilized insulin (1-10% of the amount of the native or derivatized insulin) enhanced the growth of CHO-K1 and STO cells. In addition, the maximal mitogenic effect of the immobilized insulin was greater than that of native or derivatized insulin. Photoimmobilization could be a universal means of immobilizing growth factors onto the surface of materials devoid of chemical functional groups scaffolding growth factors and providing a new protein-free cell culture system or tissue engineering materials.
Ito
Y
Y
Department of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
Chen
G
G
Imanishi
Y
Y
D000818:Animals; D001386:Azides / Q000737:chemistry; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003584:Cytological Techniques; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007328:Insulin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D010777:Photochemistry; D011137:Polystyrenes
D001386:Azides; D007328:Insulin; D011137:Polystyrenes; C087164:4-azidobenzoic acid