Mitogenic activity of peptides related to the sequence of human fibroblast growth factor-1.
Linear synthetic peptides related to the human Fibroblast Growth Factor-1 (hFGF-1) segment [112-147] were tested for their capacity of mimicking FGF mitogenic activity, binding to heparin-Sepharose columns, stimulating DNA synthesis and competing with hFGF-1 for the cellular receptors. The results obtained indicated that the activity of these compounds is dependent on the presence of the sequence WFVGLK in their structures. The affinity for the cellular receptors increased when this sequence was elongated in order to incorporate amino acid residues that are important for FGF-heparin binding.
Oyama
S
S
Departamento de Bioquímica, Universidade de São Paulo, Brasil.
Miranda
M T
MT
Toma
I N
IN
Viviani
W
W
Gambarini
A G
AG
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002847:Chromatography, Agarose; D004247:DNA / Q000096:biosynthesis; D016220:Fibroblast Growth Factor 1 / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006493:Heparin; D006801:Humans; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism
D010446:Peptide Fragments; D010455:Peptides; D011956:Receptors, Cell Surface; C103063:fibroblast growth factor-1 (112-147); D016220:Fibroblast Growth Factor 1; D006493:Heparin; D004247:DNA