Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes.
Streptomyces roseosporus mutants that express enhanced recombination between partially homologous (homeologous) sequences were isolated by selection for recombination between the bacteriophage phi C31 derivative KC570 containing the Streptomyces coelicolor glucose kinase (glk) gene and the S. roseosporus chromosome. The frequencies of homeologous recombination in the ehr mutants were determined by measuring the chromosomal insertion frequencies of plasmids containing S. coelicolor glnA or whiG genes. S. roseosporus ehr mutants showed 10(2)- to 10(4)-fold increases in homeologous recombination relative to Ehr+ strains, but no increase in homologous recombination. Southern hybridization analysis revealed single unique sites for the insertion of each of the plasmids, and the crossovers occurred in frame and in proper translational register, yielding functional chimeric glnA and whiG genes.
Hosted
T J
TJ
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, IN 46285, USA.
Baltz
R H
RH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D003434:Crossing Over, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D005941:Glucokinase / Q000235:genetics; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; C061314:whiG protein, Streptomyces coelicolor; D005941:Glucokinase; D005974:Glutamate-Ammonia Ligase