Immunocytochemical localization of the Pit-1 protein in the pituitary of the rainbow trout (Oncorhynchus mykiss).
Pit-1 is a pituitary-specific transcription factor responsible for activating the growth hormone (GH) gene family. The localization of Pit-1 protein in the pituitary of rainbow trout was studied by immunocytochemical analysis. Fish Pit-1 was found to be extensively localized in the nuclei of cells in the proximal pars distalis (PPD), rostral pars distalis (RPD), and pars intermedia (PI). Sagittal sections were immunocytochemically double stained with antibodies against Pit-1, GH, prolactin (PRL), and somatolactin (SL). The results showed that Pit-1 protein was present in the GH-containing cells of the PPD, the PRL-containing cells of the RPD, and the SL-containing cells of the PI.
Takeo
J
J
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Kitanomachi, Hachioji, Tokyo, Japan.
Yamada
S
S
Hata
J
J
Yamashita
S
S
D000818:Animals; D029941:Fish Proteins; D006023:Glycoproteins / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis; D007150:Immunohistochemistry; D017685:Oncorhynchus keta / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry*; D010907:Pituitary Hormones / Q000032:analysis*; D011388:Prolactin / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis*
D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; D014157:Transcription Factors; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone