title: Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15.
abstract: The cysteine synthase gene (cysK) from Flavobacterium K3-15 was cloned and sequenced. The gene exhibits 30-50% identity to known cysteine synthases on both the DNA and the amino acid levels. The pyridoxal phosphate binding site of the enzyme is part of a conserved motif comprising seven amino acids (SIKDRIA). The lys31 residue of the flavobacterial enzyme is conserved in all known cysteine synthases. The cysK gene from Flavobacterium K3-15 was heterologously expressed and the gene product identified by immunoblotting and determination of the enzyme activity.
authors.affiliation: Institut für Pharmazeutische Biologie, Universität Bonn, Germany. 100724.3020@compuserve.com
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: E
authors.initials: E
authors.lastname: Kuttler
authors.forename: C
authors.initials: C
authors.lastname: Lanz
authors.forename: C
authors.initials: C
authors.lastname: Drewke
authors.forename: K
authors.initials: K
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003547:Cysteine Synthase / Q000235:genetics* / Q000378:metabolism; D005417:Flavobacterium / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D003547:Cysteine Synthase