A new primer pair for detection of Chlamydia pneumoniae by polymerase chain reaction.
In order to improve the detection and identification of Chlamydia pneumoniae, new primers for polymerase chain reaction (PCR) were designed based on the DNA base sequence within the 53-kDa protein gene, which is specific for C. pneumoniae. The primers permitted the identification of 24 C. pneumoniae strains collected from different geographical locations, but no reaction was observed with C. trachomatis, C. psittaci nor C. pecorum. The primers were unable to amplify the DNA of bacteria commonly related to respiratory tract infections. The positive amplification was achieved with only 9 EBs/assay. Therefore, the new primers seem to be useful in the diagnosis of C. pneumoniae infections.
Kubota
Y
Y
Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan.
D001483:Base Sequence; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000138:chemical synthesis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005798:Genes, Bacterial; D016158:Genes, p53; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004262:DNA Restriction Enzymes