title: Inhibition of chemiluminescence response of human mononuclear cells and suppression of mitogen-induced proliferation of spleen lymphocytes of mice by hispolon and hispidin.
abstract: The effects of two phenolic compounds, hispolon and hispidin isolated from fruit bodies of the basidiomycete Inonotus hispidus (Bull. ex Fr.) Karst, were investigated on the chemiluminescence response by LPS- or zymosan-activated human mononuclear cells (MNC) and on the concanavalin A-induced proliferation of spleen lymphocytes of mouse in vitro. Both compounds showed inhibitory activity in the chemiluminescence-test with an IC50 (the concentration of test compound causing 50% effect) ranging from 4.4 to 4.6 micrograms/ml (20.3 to 21.2 microM) for hispolon and < 0.1 to 1.5 micrograms/ml (from < 0.4 microM to 6.0 microM) for hispidin. Antiproliferative effects have been achieved in the lymphocyte transformation test (LTT) by hispolon with an IC50 of 3.4 micrograms/ml (15.5 microM).
authors.affiliation: Institute of Pharmaceutical Biology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: N A
authors.initials: NA
authors.lastname: Ali
authors.forename: J
authors.initials: J
authors.lastname: Lüdtke
authors.forename: H
authors.initials: H
authors.lastname: Pilgrim
authors.forename: U
authors.initials: U
authors.lastname: Lindequist
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D008934:Mitogens / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects*; D011753:Pyrones / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
chemical_list: D002396:Catechols; D008070:Lipopolysaccharides; D008934:Mitogens; D011753:Pyrones; C103442:hispolon; D003208:Concanavalin A; D015054:Zymosan; C007946:hispidin; D013936:Thymidine