Phospholipase A2 and phospholipase D are involved in macrophage NADPH oxidase-mediated oxidation of low density lipoprotein.
Macrophage-mediated oxidation of low density lipoprotein (LDL) under oxidative stress induces activation of reduced nicotinamide adenine dinuleotide phosphate (NADPH) oxidase. Using J-774 A.1 macrophages, the present study demonstrates that phospholipase A2 (PLase A2) as well as phospholipase D (PLase D) are involved in macrophage NADPH oxidase-mediated oxidation of LDL. Furthermore, the products of these phospholipases, arachidonic acid and phosphatidic acid, can induce NADPH oxidase activation, followed by cell-mediated oxidation of LDL. This LDL oxidation was shown to be dependent on extracellular calcium ions. We conclude that PLase A2 and PLase D can induce macrophage NADPH oxidase-dependent oxidation of LDL and thus can contribute to the formation of atherogenic oxidized lipoprotein.
Aviram
M
M
Lipid Research Laboratory, Rambam Medical Center, Haifa, Israel.
Rosenblat
M
M
D016718:Arachidonic Acid / Q000276:immunology; D001161:Arteriosclerosis / Q000209:etiology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D015227:Lipid Peroxidation; D008066:Lipolysis; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008262:Macrophage Activation; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress; D010712:Phosphatidic Acids / Q000276:immunology; D010739:Phospholipase D / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2
D008077:Lipoproteins, LDL; D010712:Phosphatidic Acids; D016718:Arachidonic Acid; D019255:NADPH Oxidases; D010741:Phospholipases A; D054467:Phospholipases A2; D010739:Phospholipase D; D002118:Calcium