Differential effect of recombinant human and mouse interferons on replication of herpes simplex virus type 1 in mouse cells.
Pretreatment of murine (BALB/3T3) cells with either murine or recombinant hybrid human B/D interferon (IFN) blocked the release of infectious herpes simplex virus type 1 (HSV-1) from treated cells. The block in replication was not due to an effect on attachment of HSV-1 to the target cells or to toxic effects of IFN. Immunoblot analyses showed that murine IFN significantly reduced the expression of virus-specific proteins in IFN-treated cells. In contrast, B/D IFN had no major effect on the expression of viral proteins in treated cells. In support of the above observation, electron microscopy of virus-infected cells displayed formation of nucleocapsids within the nucleus of IFN-treated cells. However, the expression of glycoproteins B and D was reduced in B/D IFN-treated cells. These results suggested that murine IFN blocked HSV-1 replication at an early stage whereas B/D IFN inhibited HSV-1 replication at a late stage in virus morphogenesis.
Wintergerst
U
U
Department of Pediatrics, University of Alaboma at Birmingham 35294, USA.
Gangemi
J D
JD
Kern
E R
ER
Whitley
R J
RJ
Chatterjee
S
S
D016475:3T3 Cells; D000818:Animals; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D016898:Interferon-alpha / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D019251:Nucleocapsid / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011994:Recombinant Proteins; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; D014764:Viral Proteins