Electrochemical fixation techniques. I. Electrochemical fixation of human brain.
An electrochemical brain fixation procedure (EBFP) to treat brains excised from human cadavers is described thoroughly. It is as precise as any other similar method currently available. However, it takes only as much as 36 h to completion instead of the much longer lapses required by immersion in formaldehyde. Actions were taken to secure that it is not a source of artifacts of any kind, neither neurons nor glia or blood vessels. It is, therefore, amenable to be used as a valuable research and teaching tool. Other advantages are that it does not pose any health hazard, is money- and time-saving, and cuts down on equipment and facilities.
Garzon-De La Mora
P
P
División de Bioquímica-Farmacológica, Instituto Mexicano del Seguro Social, Guadalajara, México.
Garcia-Estrada
J
J
Ballesteros-Guadarrama
A
A
Navarro-Ruiz
A
A
De Jesus Macias-Comparan
J
J
Murillo-Leaño
M
M
Casillas-Ochoa
J
J
Peña-Moreno
P
P
D000328:Adult; D001923:Brain Chemistry; D002102:Cadaver; D004563:Electrochemistry / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016707:Tissue Fixation / Q000379:methods*