An attempt on using the method of R-Q double-factor analysis to identify and group fusants.
A subsequent numeric taxonomy method for identifying and grouping the fusants was explored on the basis of characterization of the protein profiles of the fusants. By these two means, several typical excellent candidates of recombinants could be searched out quickly. Among the fusants from a definite fusion-cross, the different sister-strains were regarded as the samples of observation (n = N), the positions of the all bands of protein profiles as the objects (p = P), and the photometer-scanning area of the specific band as the experimental value (X) (zero was taken when the specific band of a certain strain was absent). The genetic multirelationships among the inter- and intra-sister-strains in terms of the positions and contents of the protein bands after fusion recombination occurred from this definite fusion-cross could therefore be determined on the same orientational factor-plate by using the computer program of the R-Q double-factor method to analyze this data matrix (Xnxp). These sister-strains could then be identified and grouped from the deduced heredity relationship between the fusants and parents.
Ai
Y
Y
Institute of Microbiology, Shandong University, Jinan, China.
Meng
F
F
Gao
P
P
Wang
Z
Z
D001234:Aspergillus niger / Q000145:classification / Q000235:genetics*; D001947:Breeding / Q000379:methods; D002459:Cell Fusion; D002965:Classification / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis; D008962:Models, Theoretical; D014242:Trichoderma / Q000145:classification / Q000235:genetics*
D005656:Fungal Proteins