title: T-cell subsets and T-cell receptor V beta utilization by Igh-1-congenic mice in herpetic retinal necrosis.
abstract: BACKGROUND
  After unilateral anterior chamber (AC) inoculation with herpes simplex virus type 1 (HSV-1), C.B-17 and BALB/c congenic mice, which differ only in a limited region around the lgh-1 locus on chromosome 12, show a striking difference in susceptibility to development of encephalitis and contralateral necrotizing chorioretinitis.
  
  
  METHODS
  After AC inoculation with HSV-1 (KOS), C.B-17 and BALB/c mice were followed up for the clinical signs of encephalitis and chorioretinitis. At different time points following inoculation, lymphocytes isolated from the spleen were triple-stained with antibodies directed against CD4 or CD8, IL-2R, and various V beta T-cell receptor (TCR) subsets, and were analyzed by flow cytometry.
  
  
  RESULTS
  These lgh-1-disparate congenic mice showed differences in the time course of splenic V beta T-cell receptor (TCR) usage in both CD4+, IL-2R+ and CD8+, IL-2R+ T cells. By day 1 post infection (p.i.), C.B-17 mice showed an increase of V beta 8 and V beta 9 TCR by both CD4+, IL-2R+ and CD8+, IL-2R+ splenic T cells. Susceptible BALB/c mice delayed the increase of splenic V beta 8 and V beta 9 TCR by CD4+, IL-2R+ T cells, which was noted by day 4 p.i. Furthermore, in BALB/c mice the usage of V beta 9 by CD8+ cells was increased by day 6 p.i.
  
  
  CONCLUSIONS
  Our findings indicate that early preferential splenic usage of a restricted repertoire of TCR occurs after ocular inoculation with HSV-1 in resistant C.B-17 mice. Such preferential TCR usage by activated T cells may prevent viral replication in the brain and contralateral eye and may be linked to protection from development of encephalitis and destructive herpesmediated ocular inflammation.
authors.affiliation: Hilles Immunology Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, USA.
authors.forename: A
authors.initials: A
authors.lastname: Berra
authors.forename: A
authors.initials: A
authors.lastname: Heiligenhaus
authors.forename: C S
authors.initials: CS
authors.lastname: Foster
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002825:Chorioretinitis / Q000276:immunology / Q000473:pathology; D018792:Encephalitis, Viral / Q000276:immunology / Q000473:pathology; D015828:Eye Infections, Viral / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D006561:Herpes Simplex / Q000209:etiology / Q000276:immunology / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D015882:Retinal Necrosis Syndrome, Acute / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D007143:Immunoglobulin Heavy Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2