title: Axonal growth-related cell surface molecule, neurin-1, involved in neuron-glia interaction.
abstract: We purified and characterized a novel axonal growth-related molecule, neurin-1, which is anchored to the surface membrane via a phosphatidylinositol (PI) linkage. This molecule was detected by a combination of phosphatidylinositol-specific phospholipase C (PI-PLC) treatment from detergent-soluble mouse brain membranes and subsequent Western blot analysis with monoclonal antibody (MAb 2A). Neurin-1 is immunologically distinct from other known axonal growth associated surface glycoproteins. In immunoblots of embryonic mouse brain membrane, the MAb 2A recognized a single band at approximately 68 kDa, and showed that neurin-1 is mainly associated with fiber-containing regions of developing embryonic mouse brain. Expression is immunohistochemically similar to that of cell adhesion molecule L1, but in comparison, neurin-1 appears somewhat later. Late in embryonic development, neurin-1 appeared to be more stage- and region-specific. Its precise localization at the neural cell surface membranes was confirmed by immuno-electron microscopy using labeled and cultured live nerve cells. Neurin-1 was found only on the surface of the axon and growth cone. Neurin-1, otherwise termed PI anchor protein, corresponds closely in function to the other PI-anchored cell adhesion molecules. Anti-neurin-1 antibody (MAb 2A), however, perturbs the axonal growth and neural cell migration from the astrocyte feeder layer cultures. These results suggest that neurin-1 is one of the important cell surface molecules mediated in the neuron and glial cell interaction.
authors.affiliation: Department of Physiology Keio University, School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Asou
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: I
authors.initials: I
authors.lastname: Uemura
authors.forename: M
authors.initials: M
authors.lastname: Sugawa
authors.forename: K
authors.initials: K
authors.lastname: Uyemura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000502:physiology*; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D019062:Cell Adhesion Molecules, Neuron-Glia / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008566:Membranes / Q000201:enzymology; D051379:Mice; D016253:Microscopy, Immunoelectron; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D019062:Cell Adhesion Molecules, Neuron-Glia; C103810:neurin-1