title: Aldosterone-induced proteins in primary cultures of rabbit renal cortical collecting system.
abstract: Primary cultures of immunodissected cells from rabbit kidney connecting tubule and cortical collecting duct were used to study aldosterone's action on transcellular Na+ flux. Incubation with 10(-7) M aldosterone stimulated transcellular Na+ transport which was detected as an increase in benzamil-sensitive short-circuit current. The stimulatory response was consistently noted after 2 h of incubation and stabilized after 6 h. 2D-PAGE was used to identify proteins which were induced concurrently with the increase in transcellular Na+ flux after an aldosterone incubation of 15 h. Three aldosterone-induced proteins (AIPs; M(r) = 100, 70-77 and 46-50 kDa) were found in the membrane and microsomal fractions. Two of these appeared to have more than one isoform. A single heterogeneous AIP (M(r) = 77 kDa) was detected in the soluble fraction.
authors.affiliation: Department of Cell Physiology (162), University of Nijmegen, The Netherlands. reneb@sci.kun.nl
authors.forename: R J
authors.initials: RJ
authors.lastname: Bindels
authors.forename: A M
authors.initials: AM
authors.lastname: Engbersen
authors.forename: A
authors.initials: A
authors.lastname: Hartog
authors.forename: B L
authors.initials: BL
authors.lastname: Blazer-Yost
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D017136:Ion Transport / Q000187:drug effects; D007672:Kidney Cortex / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism
chemical_list: D011506:Proteins; D015222:Sodium Channels; D000450:Aldosterone; D012964:Sodium