title: [Adaptation of the measurement of plasma and erythrocyte glutathione peroxidase activity on Hitachi 911].
abstract: The aim was to automate the assay of plasma and erythrocyte glutathione peroxidase (GSH-Px) activity using the Hitachi 911 (Boehringer Mannheim). There is a need for a reliable and rapid technique for the determination of GSH-Px activity to evaluate 'the oxidative stress'. The technique used was that proposed by the Randox laboratories (Randox Laboratories Ltd, Ransel test, reference: RS-505). The Hitachi 911 was programmed by adapting the manual Randox technique. Repeatability, reproducibility and linearity were satisfactory, and usual values for plasma and erythrocyte GSH-Px activity were determined. Automation of the Ransel test for determination of GSH-Px activity was very useful for serial assays. In addition, the technique is practical and has good interlaboratory reproducibility.
authors.affiliation: Laboratoire de biochimie, hôpital de la Salpêtrière, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Dever
authors.forename: C
authors.initials: C
authors.lastname: Vegas
authors.forename: F
authors.initials: F
authors.lastname: Imbert-Bismut
authors.forename: J
authors.initials: J
authors.lastname: Delattre
authors.forename: D
authors.initials: D
authors.lastname: Bonnefont-Rousselot
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002624:Chemistry, Clinical / Q000295:instrumentation*; D004912:Erythrocytes / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D005979:Glutathione Peroxidase