title: Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis.
abstract: The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtraction are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, 1993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: O D
authors.initials: OD
authors.lastname: Ermolaeva
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D016678:Genome; D006801:Humans; D007700:Kinetics; D051379:Mice; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013382:Subtraction Technique; D014998:Y Chromosome
chemical_list: D004247:DNA