title: SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae.
abstract: The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.
authors.affiliation: Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Cliften
authors.affiliation: Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Yeelan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Fermentation Technology, Hiroshima University, 1-4-1 Higashi-Hiroshima 739, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Mochizuki
authors.affiliation: Department of Fermentation Technology, Hiroshima University, 1-4-1 Higashi-Hiroshima 739, Japan.
authors.forename: Tokichi
authors.initials: T
authors.lastname: Miyakawa
authors.affiliation: Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Rungrach
authors.initials: R
authors.lastname: Wangspa
authors.affiliation: Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Joanne
authors.initials: J
authors.lastname: Hughes
authors.affiliation: Department of Biology and Program in Molecular Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Jon Y
authors.initials: JY
authors.lastname: Takemoto
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D009154:Mutation; D011549:Pseudomonas / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C032677:syringomycin; D006899:Mixed Function Oxygenases; C109313:SUR2 protein, S cerevisiae