mRNA for NGF and p75 in the central nervous system of rats affected by experimental allergic encephalomyelitis.
In this study we measured the concentration of nerve growth factor (NGF) and the expression of NGF and the low affinity NGF-receptor (NGF-r) mRNA in the central nervous system (CNS) of rats affected by experimental allergic encephalomyelitis (EAE) during the acute phase of the disease. Significant levels of NGF protein were found in thalamus and cortex on day 13 post-immunization. Molecular analysis of the NGF gene expression and of its NGF-r revealed that they were enhanced in several regions of the CNS of EAE rats when compared with untreated animals. These results suggest a functional link between local NGF synthesis and this autoimmune inflammatory disease.
De Simone
R
R
Institute of Neurobiology, CNR, Rome, Italy.
Micera
A
A
Tirassa
P
P
Aloe
L
L
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D009414:Nerve Growth Factors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D013788:Thalamus / Q000378:metabolism*; D014018:Tissue Distribution
D009414:Nerve Growth Factors; D012333:RNA, Messenger