A novel method for glycoconjugate synthesis.
2-Chloroethyl 1-thio-beta-D-galactopyranoside and the corresponding 1-thio-beta-D-glucopyranoside have been found to be suitable glycosylating agents for the preparation of different types of glycoconjugates. Glycosylation of bovine serum albumin and chymotrypsin were chosen as examples of an application of the described compounds. The glycosylating agents can modify not only amino groups, but also alkyl hydroxyl and aryl hydroxyl groups, as was shown in experiments with model water soluble and water insoluble polyacrylamide copolymers.
Tichá
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M
Department of Biochemistry, Charles University, Praha, Czech Republic.
Cerný
M
M
Trnka
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T
D002918:Chymotrypsin / Q000737:chemistry; D006001:Glycoconjugates / Q000138:chemical synthesis*; D006027:Glycosides / Q000737:chemistry; D006031:Glycosylation; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine / Q000737:chemistry
D006001:Glycoconjugates; D006027:Glycosides; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D002918:Chymotrypsin