A human kidney cDNA which induces a cell surface protein epitope recognized by a monoclonal antibody against galactosylceramide.
Antibodies against the myelin glycolipid galactosylceramide are widely used to study the distribution and function of this molecule. However, anti-galactosylceramide antibodies are not monospecific and have been shown to recognize epitopes carried not only by other glycolipids, but also by proteins. Using expression cloning we have identified a human kidney cDNA which induces a cell-surface protein recognized by the anti-galactosylceramide monoclonal antibody R-mab. These findings further support the idea that cross-reactive proteins may mediate some of the biological effects of the anti galactosylceramide antibodies.
Sosa
M A
MA
Department of Neurology, New York University School of Medicine, New York 10016, USA.
De Gasperi
R
R
Battistini
S
S
Gorman
M P
MP
Kolodny
R
R
Kolodny
E H
EH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D018076:DNA, Complementary; D000939:Epitopes / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D005699:Galactosylceramides / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D000939:Epitopes; D005699:Galactosylceramides; D008565:Membrane Proteins; D011994:Recombinant Proteins