title: Transferrin and its receptor in the development of genetically determined neural tube defects in the mouse embryo.
abstract: The iron-binding growth factor transferrin is taken up and localised in the hindgut of midgestation mouse embryos. We investigated whether the distribution of transferrin may be disturbed in mutant curly tail embryos, a proportion of which exhibit a cell proliferation defect affecting the hindgut endoderm, as part of the pathogenetic sequence leading to development of neural tube defects. Immunostaining revealed a reduction in the binding and/or uptake of transferrin by hindgut epithelial cells in affected curly tail embryos compared with their unaffected littermates. There was no apparent difference between the two embryo types, however, in the distribution or level of expression of the transferrin receptor. The receptor is expressed specifically in the hindgut endoderm of the 10.5-day embryo, although its mRNA is present in all tissues of the posterior neuropore region, suggesting posttranscriptional control of gene expression. These findings may indicate a role for transferrin binding and/or uptake in the regulation of cell proliferation in the hindgut endoderm, with a defect in this process in the curly tail mutant. However, an alternative explanation is suggested by our finding that transferrin immunostaining is more intense in the hindgut of unaffected curly tail embryos than in nonmutant CBA/Ca and CD-1 embryos. Thus, mutant embryos may increase their uptake of transferrin in an attempt to compensate for defective cell proliferation in the hindgut resulting from a defect in another pathway. Only a proportion of embryos are able to mount this compensatory response leading to the observed partial penetrance of developmental defects in the curly tail mutant mouse.
authors.affiliation: Neural Development Unit, University of London, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Hoyle
authors.forename: D J
authors.initials: DJ
authors.lastname: Henderson
authors.forename: D J
authors.initials: DJ
authors.lastname: Matthews
authors.forename: A J
authors.initials: AJ
authors.lastname: Copp
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology; D005260:Female; D005434:Flow Cytometry; D017403:In Situ Hybridization; D051379:Mice; D008817:Mice, Mutant Strains; D009436:Neural Tube Defects / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D014168:Transferrin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D011990:Receptors, Transferrin; D014168:Transferrin