title: Effects of combinations of stem cell factor and hemin on erythropoiesis after azidothymidine treatment of immunosuppressed mice.
abstract: Recombinant cytokines such as stem cell factor (SCF) are currently being tested for the ability to ameliorate 3'azido-3'deoxythymidine (AZT) induced anemia in AIDS patients. Recently, we demonstrated that SCF and hemin in vitro greatly increased the resistance of burst-forming units erythroid (BFU-E) to AZT. We therefore attempted to ameliorate AZT-induced anemia in vivo using SCF and hemin in immunodeficient LP-BM5 infected (MAIDS) mice. SCF and hemin were administered with oral AZT for 3 wk and the effects on erythropoiesis examined. Hemin significantly increased hematocrits of AZT-treated mice and control mice. However, SCF and SCF-hemin combinations failed to raise hematocrits. Reticulocyte numbers were significantly consistently increased only in hemin-treated mice receiving AZT. The numbers of CFU-E were increased in bone marrow of AZT-treated mice receiving hemin. Therefore, SCF did not enhance the erythropoietic effect of hemin in AZT-treated immunodeficient mice.
authors.affiliation: University of Maryland Cancer Center, Baltimore 21201, USA.
authors.forename: A W
authors.initials: AW
authors.lastname: Hamburger
mesh_terms: D000740:Anemia / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D004920:Erythropoiesis / Q000187:drug effects*; D006400:Hematocrit; D006427:Hemin / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000503:physiopathology*; D019089:Stem Cell Factor / Q000494:pharmacology* / Q000627:therapeutic use; D015215:Zidovudine / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity
chemical_list: D019089:Stem Cell Factor; D015215:Zidovudine; D006427:Hemin