Lung cancer-reacting human recombinant antibody AE6F4: potential usefulness in the sputum cytodiagnosis.
Human monoclonal antibody (hMAb) AE6F4 has been shown to be potentially useful for immunocytological detection of lung cancer cells in sputum. By recombinant DNA technology, IgM type hMAb AE6F4 was switched to lgG. The IgG mimic recombinant AE6F4 antibody expression plasmid was assembled using the antibody heavy chain gene, which ligated the gene encoding VH and CH1(mu) domains of hMAb AE6F4 heavy chain to the gene encoding CH2(gamma 1) and CH3(gamma 1) domains of human IgG heavy chain, and the antibody light chain gene of hMAb AE6F4. The recombinant antibody expressed by baby hamster kidney (BHK)-21 cells showed molecular size equivalence to IgG, and consisted of human mu-gamma hybrid heavy and kappa light chains. The immunological specificity of the recombinant antibody was the same as that of hMAb AE6F4 by immunoblotting analysis to the 14-3-3 protein, the putative antigen of hMAb AE6F4, and by immunohistochemical and immunocytological analyses using tissue sections and sputa of lung cancer patients. The transfected BHK-21 cells produced the recombinant antibody persistently and the productivity was greater than 20 times that by human-human hybridoma producing hMAb AE6F4.
Shoji
M
M
Morinaga Institute of Biological Science, Yokohama, Japan.
Kawamoto
S
S
Seki
K
K
Teruya
K
K
Setoguchi
Y
Y
Mochizuki
K
K
Kato
M
M
Hashizume
S
S
Hanagiri
T
T
Yoshimatsu
T
T
Nakanishi
K
K
Yasumoto
K
K
Nagashima
A
A
Nakahashi
H
H
Suzuki
T
T
Imai
T
T
Shirahata
S
S
Nomoto
K
K
Murakami
H
H
D048948:14-3-3 Proteins; D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000276:immunology; D010957:Plasmids; D011506:Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D013183:Sputum / Q000276:immunology*; D014162:Transfection; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D007074:Immunoglobulin G; D011506:Proteins; D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase