The effect of pretreatment with endotoxin and lactobacillus on bacterial translocation in acute liver injury.
OBJECTIVE
To evaluate the effect of pretreatment with endotoxin or Lactobacillus reuteri pretreatment on bacterial translocation after acute liver injury.


METHODS
Experimental study.


METHODS
University department, Sweden.


METHODS
96 Sprague-Dawley rats were divided into four groups of 24 each. Three of them received intraperitoneal D-galactosamine, the fourth received pyrogen free water and was used as the normal control. Twenty-four hours later the study was terminated and samples collected.


METHODS
Endotoxin and L reuteri R2LC were injected intraperitoneally three days, one week, and two weeks before induction of liver injury.


METHODS
Extent of liver injury and bacterial translocation to mesenteric lymph nodes, liver, and systemic blood.


RESULTS
The extent of liver injury and rate of bacterial translocation were lower three days after pretreatment with endotoxin, than after pretreatment with L reuteri. There was no other difference among the other groups. High concentrations of serum endotoxin were detected three days after pretreatment with endotoxin. There were no significant changes in small intestinal and caecal bacterial counts.


CONCLUSIONS
Pretreatment with endotoxin effectively prevented liver injury by D-galactosamine and subsequent bacterial translocation. Pretreatment with L reuteri had no beneficial effect.
Kasravi
F B
FB
Department of Surgery, Lund University, Sweden.
Adawi
D
D
Hägerstrand
I
I
Molin
G
G
Bengmark
S
S
Jeppsson
B
B
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D056486:Chemical and Drug Induced Liver Injury; D004731:Endotoxins / Q000097:blood / Q000494:pharmacology*; D005688:Galactosamine; D007778:Lactobacillus; D008107:Liver Diseases / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D004731:Endotoxins; D005688:Galactosamine