title: Molecular cloning, structural analysis, and expression of carp ZP3 gene.
abstract: Two types of cDNAs coding for a major component of carp egg membrane were clones from a carp ovarian cDNA library. They encode polypeptides of 422-424 amino acid residues whose sequences are homologous to those of medaka and mammalian ZP3. Similar to the mammalian ZP3 genes, carp ZP3 gene also consists of eight exons and seven introns. Carp ZP3 genes are 2.9 kb in length and present in multiple forms. Carp ZP3 is a glycoprotein of 45 kDa. It was transcribed and translated exclusively in oocytes, in contrast with medaka ZP3, which was synthesized in liver. The transcription of carp ZP3 starts very early in oogenesis, but translation occurs during vitellogenesis, as it is present in vitellogenic but not in previtellogenic oocytes. ZP3 content in oocytes increases as vitellogenesis proceeds.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Y S
authors.initials: YS
authors.lastname: Chang
authors.forename: S C
authors.initials: SC
authors.lastname: Wang
authors.forename: C C
authors.initials: CC
authors.lastname: Tsao
authors.forename: F L
authors.initials: FL
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004527:Egg Proteins / Q000235:genetics*; D015870:Gene Expression; D006054:Goldfish / Q000235:genetics; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009865:Oocytes; D010063:Ovum; D012333:RNA, Messenger; D011817:Rabbits; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014818:Vitellogenesis; D015044:Zona Pellucida; D000072216:Zona Pellucida Glycoproteins
chemical_list: D018076:DNA, Complementary; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins