title: Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids.
abstract: Degenerate primers based on human and mouse obesity gene (OBS) sequencing data were used in the reverse transcriptase-polymerase chain reaction (RT-PCR) of total RNA from pig white adipose tissue. Both strands of the resultant pig- specific 325 bp DNA fragment were sequenced. Comparison of the obtained sequence with known sequences revealed an 86% identity with the human and 84% identity with the mouse OBS cDNA. The OBS gene was physically mapped to pig chromosome 18 by PCR analysis of somatic cell hybrids, using pig-specific primers. This result is consistent with the recent assignment of the human OBS gene to chromosome 7 and the observation made by comparative mapping that by using a human chromosome 7 specific library two segments of conserved synteny were detected on porcine chromosomes 9 and 18. We conclude the border of conserved synteny to be in the 7q31-7q32 region of the human chromosome.
authors.affiliation: Department of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum, Zürich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Neuenschwander
authors.forename: G
authors.initials: G
authors.lastname: Rettenberger
authors.forename: E
authors.initials: E
authors.lastname: Meijerink
authors.forename: H
authors.initials: H
authors.lastname: Jörg
authors.forename: G
authors.initials: G
authors.lastname: Stranzinger
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D020738:Leptin; D051379:Mice; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D020738:Leptin; D011506:Proteins