Identification and characteristic of LDL-subfractions in human plasma.
Density gradient ultracentrifugation and high resolution polyacrylamide gel electrophoresis (Lipoprint LDL-system) were used to examine the discrete LDL subfraction patterns in normal and hyperlipidemic patients. Two new programs for LDL and VLDL subfractions were elaborated and a new method of evaluation for cholesterol content in LDL-subfractions, based on area distribution ratio of densitograms without density gradient ultracentrifugation, was created. The predominance of cholesterol and triglycerides in dense LDL3-subclass (d-1,048-1,063 g/ml) under hypercholesterolemia and hypertriglyceridemia was found. Lipoprint LDL-electrophoresis method was developed for the characteristic of lipemic state of organism and detection and quantitation of plasma LDL subfractions.
Kholodova
Y D
YD
University of Kansas Medical Center, Kansas City, USA.
Harris
W S
WS
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood* / Q000145:classification; D008079:Lipoproteins, VLDL / Q000097:blood; D014461:Ultracentrifugation
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL