Localization and expression of phospholipases A2 in Trimeresurus flavoviridis (habu snake) venom gland.
The localization and expression profiles of phospholipases A2 (PLA2s) in Trimeresurus flavoviridis venom gland were studied by means of in situ hybridization and immunohistochemical techniques. Venom gland cells are tightly arrayed in a single layer along the inlet-like lumens in which venom proteins are stored. mRNAs for PLA2s were detected at the high level in cytoplasm. Using the immunohistochemical technique with polyclonal anti-Asp-49-PLA2 antibody, Asp-49-PLA2 and, possibly, its isozymes were detected in intracellular granules and in venom lumens. The intracellular granules containing PLA2 proteins appear to be transferred from the nucleus towards the outer membrane facing the lumen, and then to be secreted.
Ogawa
T
T
Department of Chemistry, Kyushu University, Fukuoka, Japan.
Onoue
H
H
Nakagawa
K
K
Nomura
S
S
Sueishi
K
K
Hattori
S
S
Kihara
H
H
Ohno
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005088:Exocrine Glands / Q000201:enzymology*; D006416:Hematoxylin / Q000737:chemistry; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000378:metabolism; D012910:Snake Venoms / Q000201:enzymology* / Q000235:genetics; D013194:Staining and Labeling; D017841:Trimeresurus / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D012910:Snake Venoms; D010741:Phospholipases A; D054467:Phospholipases A2; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin