The B2 receptor on cultured human decidua cells: release of arachidonic acid by bradykinin.
In vitro studies on cultured human decidual cells present evidence for the expression of the bradykinin B2 receptor. This protein was localized by immunocytochemical methods at the cellular plasma membrane and the mRNA was found in cellular extracts by RT-PCR. As a biological effect the release of 14C-AA was observed. Cells that had been incubated for 24 h with 14C-AA showed a bradykinin (BK)-induced release of radioactivity into the culture medium. This response was prevented by the specific bradykinin B2 receptor antagonist HOE 140. Pretreatment with LPS resulted in an increase of the spontaneous release of radioactivity. Adding BK, a further release of about 40-50% was to be shown. We conclude that BK can play a role in the case of bacterial infection of the fetal membranes AND may stimulate labour by an augmented production of prostaglandins.
Rehbock
J
J
I. Frauenklinik der LMU, München, Germany.
Chondromatidou
A
A
Buchinger
P
P
Hermann
A
A
D016718:Arachidonic Acid / Q000378:metabolism*; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology*; D065168:Bradykinin Receptor Antagonists; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000235:genetics / Q000378:metabolism*
D065168:Bradykinin Receptor Antagonists; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D016718:Arachidonic Acid; C065679:icatibant; D001920:Bradykinin