Presence of a hypervariable region within the hr2 domain of the host range determining sequences of the envelope protein gp85 (SU) of subgroup-A avian sarcoma-leukosis viruses.
The nucleotide sequence of the gene for the envelope protein gp85 (SU) of the Schmidt-Ruppin subgroup A (NY) strain of Rous sarcoma virus [SRA(NY)] was determined, and the deduced amino acid sequence was compared with those of other avian sarcoma-leukosis viruses. Among the five host-range determinant sequences (vr1, vr2, hr1, hr2, and vr3), the host-range determinant sequence hr2 of SRA(NY) showed a significant deviation from the hr2 sequences of other subgroup A viruses namely, RAV-1 and SRA(SF). A phylogenetic analysis of the amino acid sequence of this region indicated that this intra-subgroup diversity was as great as or even greater than the inter-subgroup diversity found among other subgroups of ASLV. Within the hr2 region, we found a short hypervariable segment that differs in length and sequence from hr2 of other subgroup-A viruses. The difference in the hr2 amino acid sequence between SRA(NY) and SRA(SF) is reflected in the predicted protein secondary structure of this region.
Hara
H
H
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA. kaji@cbil.humgen.upenn.edu
Tanaka
A
A
Kaji
A
A
D000595:Amino Acid Sequence; D001354:Avian Leukosis Virus / Q000235:genetics*; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D014759:Viral Envelope Proteins / Q000235:genetics*
C062389:Rous sarcoma virus envelope protein gp85; D014759:Viral Envelope Proteins