Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro.
The effects of exogenous and endogenous granulocyte colony-stimulating factor (G-CSF) on invasion by cancer cells were studied, using lung cancer cell lines that produce G-CSF (NCI-H157) and lines that do not (PC-9 and NCI-H23). The invasive capacity of NCI-H157 cells was 26- to 27-fold higher than that of PC-9 and NCI-H23 cells. The invasiveness of PC-9 cells was stimulated by exogenous G-CSF, while that of NCI-H157 cells was not. Antibodies against G-CSF blocked the stimulation of PC-9 cell invasiveness by exogenous G-CSF. Anti G-CSF antibodies also inhibited invasion by NCI-H157 cells in the absence of exogenous G-CSF. These results suggest that endogenous and exogenous G-CSF both stimulate invasion by lung cancer cells.
Pei
X H
XH
Research Institute for Diseases of the Chest, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nakanishi
Y
Y
Takayama
K
K
Yatsunami
J
J
Bai
F
F
Kawasaki
M
M
Wakamatsu
K
K
Tsuruta
N
N
Mizuno
K
K
Hara
N
N
D000906:Antibodies / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D000906:Antibodies; D016179:Granulocyte Colony-Stimulating Factor