title: Cytokine priming of human basophils: description of allergen 'nonreleasers'.
abstract: Interleukins 3 and 5 and GM-CSF enhance histamine release from basophils triggered by various stimuli. In this report, we describe a subset of allergic patients whose basophils release histamine in response to allergen only when primed with cytokine. In the absence of cytokine, there is no detectable response to allergen. These patients, who represent 4-13% of the allergic population, cannot be distinguished by skin test reactivity or severity of allergic symptoms. Allergen nonreleasers tend to have lower titers of allergen-specific IgE than the majority of atopic subjects, but this difference is not significant (average titer of 29.8 for nonreleasers vs. 188 for typical allergies; p = 0.15). They release histamine normally with anti-IgE and with fMLP, indicating that basophils are responsive to signalling through the IgE receptor, and there is no intrinsic defect in degranulation. Thus, in these patients, the IgE-mediated release of inflammatory mediators from basophils is dependent on, rather than merely enhanced by, T cell cytokines. The relationship between these patients and the previously described anti-IgE 'nonreleasers' is discussed.
authors.affiliation: ImmuLogic Pharmaceutical Corporation, Waltham, Mass 02154, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Black
authors.forename: A M
authors.initials: AM
authors.lastname: Lussier
authors.forename: W R
authors.initials: WR
authors.lastname: Gion
authors.forename: M T
authors.initials: MT
authors.lastname: Kasaian
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D007377:Interleukin-3 / Q000235:genetics / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology; D017455:Receptors, IgE / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007377:Interleukin-3; D015848:Interleukin-5; D017455:Receptors, IgE; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D009240:N-Formylmethionine Leucyl-Phenylalanine