title: Accelerated Biodegradation of High and Low Concentrations of p-Nitrophenol (PNP) by Bacterial Inoculation in Industrial Wastewater: The Role of Inoculum Size on Acclimation Period
abstract: The effect of inoculum size on the acclimation period and rate and extent of p-nitrophenol (PNP) degradation at high (1-10 mg/L) and low (26 &mgr;g/L) concentrations for two bacteria was determined in defined media as well as industrial wastewater. Increased inoculum size did not affect the acclimation period of either bacterium at high (1-10 mg/L) PNP concentrations. At low PNP concentrations (26 &mgr;g/L), the two bacteria behaved differently. The acclimation period was shortened and both the rate and extent of mineralization of PNP were enhanced by increasing the Corynebacterium sp. inoculum size from 3 x 10(5) to 3 x 10(6) cells/ml. Addition of phosphate or elimination of predators also reduced the acclimation period. Conversely, increasing the inoculum size from 3 x 10(5) to 5 x 10(6) cells/ml of Pseudomonas putida lengthened the acclimation period and reduced both the rate and extent of mineralization. It is suggested that, in a given environment, the success of an introduced species to enhance the degradation of a chemical depends upon (i) concentration of the chemical, (ii) selection of an appropriate microorganism, and (iii) utilization of a suitable inoculum size.
authors.affiliation: Department of Marine Sciences, University of Puerto Rico, Mayaguez, Puerto Rico 00681-5000
authors.initials: BR
authors.lastname: Zaidi
authors.initials: SH
authors.lastname: Imam
authors.initials: RV
authors.lastname: Greene