Ex vivo study of skin phototypes.
We studied skin phototypes ex vivo to validate a model of epidermal reconstruction with melanocytes. We made autologous epidermal reconstructs with keratinocytes and melanocytes of healthy donors of skin phototypes I to VI. Keratinocytes and melanocytes were seeded on a dead de-epidermized dermis (Pruniéras type) at a 1:20 melanocyte/keratinocyte ratio. Reconstructed epidermis was grown for 15 d at the air-liquid interface with or without ultraviolet B irradiation. A macroscopic, chromometric. histologic, and ultrastructural evaluation was performed. Reconstructs reproduced the initial phototype with few modifications. The intensity of melanin transfer correlated with the in vivo situation and was stimulated after ultraviolet B irradiation in reconstructs of all categories of skin phototypes.
Bessou
S
S
Laboratoire de Dermatologie, Université Victor Ségalen Bordeaux II, France.
Surlève-Bazeille
J E
JE
Pain
C
C
Donatien
P
P
Taïeb
A
A
D000328:Adult; D004295:Dihydroxyphenylalanine / Q000032:analysis; D006801:Humans; D008544:Melanocytes / Q000648:ultrastructure; D012867:Skin / Q000166:cytology* / Q000528:radiation effects / Q000648:ultrastructure; D012880:Skin Pigmentation; D014466:Ultraviolet Rays
D004295:Dihydroxyphenylalanine