Sequence analysis of PG10.2, a gene expressed in the pineal gland and the outer nuclear layer of the retina.
A cDNA (PG10.2) was cloned from rat using RNA fingerprinting by arbitrarily primed PCR (AP-PCR). Initially, a 145-bp DNA fragment was isolated from a pineal-specific band revealed on a sequencing gel. Riboprobes generated from the DNA fragment were used for hybridization histochemical and Northern analyses. Both techniques indicate a gene (8 kb mRNA) expressed only in the pineal gland and the outer nuclear layer of the retina. Two 4-kb cDNA fragments generated by 5'-RACE (rapid amplification of cDNA ends) and 3'-RACE were obtained using a long template PCR-based RACE technique. DNA sequencing revealed a single long open reading frame (ORF) encoding a predicted protein of 1239 amino acids, containing multiple motifs: a typical signal sequence of 20 amino acids at the NH2-terminus, a long extracellular domain with multiple potential glycosylation sites, a 31-amino-acid transmembrane domain near the COOH-terminus and a 109-amino-acid cytoplasmic tail. There are also two domains similar, but not identical, to EGF-like (epidermal growth factor-like) domains located just upstream of the transmembrane domain. These features suggest that this gene may encode a glycoprotein involved in cell adhesion, growth, and differentiation of pineal and retinal photoreceptor cells.
Wang
X
X
Laboratory of Cell Biology, NIMH, Bethesda, MD 20892-4068, USA.
Brownstein
M J
MJ
Young
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004815:Epidermal Growth Factor / Q000737:chemistry; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D015723:Gene Library; D005796:Genes; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010870:Pineal Gland / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011509:Proteoglycans; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D005136:Eye Proteins; C504416:Impg2 protein, rat; D011509:Proteoglycans; D004815:Epidermal Growth Factor