Effect of induced hypertension with angiotensin II infusion on biodistribution of 111in-labeled monoclonal antibody.
We investigated whether induced hypertension could enhance tumor uptake of monoclonal antibody. 111In-DTPA-A7 (IgG1 against 40kD tumor associated glycoprotein) was injected into colon carcinoma xenografted mice which were subcutaneously implanted with micro-osmotic pump containing angiotensin II (AT-II). Biodistribution was observed in groups of mice infused with AT-II at rate of 0.5 micrograms/kg/min (L) or 1 microgram/kg/min (H) and compared with a group of mice infused with saline (S). Tumor uptake of 111In-A7 in L and H was 1.32 and 1.57 times greater than S at 48 h after intravenous injection of A7. Normal organ uptakes also tended to be increased by AT-II infusion. Further study is needed to get optimum effect of hypertensive treatment on biodistribution of radiolabeled MoAb.
Kinuya
S
S
Department of Nuclear Medicine, Kanazawa University School of Medicine, Ishikawa, Japan.
Yokoyama
K
K
Konishi
S
S
Tonami
N
N
Hisada
K
K
D000804:Angiotensin II / Q000008:administration & dosage; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism*; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D007262:Infusions, Intravenous; D007553:Isotope Labeling; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D012709:Serum Albumin; D013667:Technetium; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D012709:Serum Albumin; D000804:Angiotensin II; D013667:Technetium