title: Genotypic characterization of sequential Aspergillus fumigatus isolates from patients with cystic fibrosis.
abstract: Twenty-three sequential Aspergillus fumigatus sputum isolates, which had been collected over a period of 2 years, from two patients with cystic fibrosis were genotyped by random amplified polymorphic DNA PCR and restriction fragment length polymorphism analysis. In patient B, one genotype was predominantly present in the sputum samples, while in the other patient up to nine different genotypes were identified. This study suggests that different patterns of colonization with A.fumigatus exist in patients with cystic fibrosis.
authors.affiliation: Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
authors.forename: P E
authors.initials: PE
authors.lastname: Verweij
authors.forename: J F
authors.initials: JF
authors.lastname: Meis
authors.forename: J
authors.initials: J
authors.lastname: Sarfati
authors.forename: J A
authors.initials: JA
authors.lastname: Hoogkamp-Korstanje
authors.forename: J P
authors.initials: JP
authors.lastname: Latgé
authors.forename: W J
authors.initials: WJ
authors.lastname: Melchers
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal