Evaluation of anti-human antibodies for immunohistochemistry on archival nonhuman primate tissues.
A panel of commercially available antibodies which recognize specific antigens on human tissues was developed for use in immunohistochemistry on tissues from eight species of nonhuman primates. Antibodies were selected for potential usefulness in diagnostic pathology, and for effectiveness in formalin-fixed, paraffin-embedded tissues. Tissues from four species of macaques and four New World monkeys were evaluated. Using these antibodies we were able to identify 17/21 antigens examined in all eight species, and 21/21 antigens in the four species of macaques. Detailed immunohistochemistry protocols are presented, along with a systematic approach to developing a protocol for a new antibody.
Wykrzykowska
J J
JJ
Division of Comparative Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA, USA.
Pauley
D R
DR
Lackner
A A
AA
Simon
M A
MA
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002520:Cercopithecidae; D006680:HLA Antigens / Q000032:analysis / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008251:Macaca; D011323:Primates / Q000276:immunology*; D013045:Species Specificity
D000906:Antibodies; D000941:Antigens; D006680:HLA Antigens