title: Stimulation of fibronectin synthesis through the protein kinase C signalling pathway in normal and transformed human lung fibroblasts.
abstract: We examined the role of the protein kinase C (PKC) signaling pathway in the stimulation of fibronectin synthesis in both normal and transformed human lung fibroblasts. Phorbol myristate acetate (PMA), a potent PKC activator, stimulated fibronectin synthesis in both normal and transformed fibroblasts in a time and dose dependent fashion. Down-regulation of PKC by prior exposure of cells to a high concentration of PMA blocked the increase in fibronectin synthesis and mRNA levels induced by PMA. Bisindolylmaleimide, a specific inhibitor of PKC, also abolished the PMA-induced fibronectin synthesis. 4 alpha-phorbol didecanoate, an inactive phorbol ester, failed to affect fibronectin synthesis. These data suggest that PMA stimulates fibronectin synthesis and gene expression through the PKC signaling pathway in both normal and transformed human lung fibroblasts.
authors.affiliation: Department of Biochemistry, School of Medicine, Kyungpook National University, Taegu, Korea.
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
authors.forename: R W
authors.initials: RW
authors.lastname: Park
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: H M
authors.initials: HM
authors.lastname: Ryoo
authors.forename: K Y
authors.initials: KY
authors.lastname: Sohn
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
mesh_terms: D002273:Carcinogens / Q000494:pharmacology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D008301:Maleimides / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D005353:Fibronectins; D007211:Indoles; D008301:Maleimides; D010703:Phorbol Esters; D012333:RNA, Messenger; C008876:phorbol-12,13-didecanoate; D011493:Protein Kinase C; C088060:bisindolylmaleimide; D013755:Tetradecanoylphorbol Acetate