title: Efficient gene transfer to EGF receptor overexpressing cancer cells by means of EGF-labeled cationic liposomes.
abstract: Epidermal growth factor (EGF)-labeled cationic liposomes (EGF-liposomes) were prepared for efficient gene transfer vector to EGF receptor expressing cells. Transfection activity of EGF-liposomes associating plasmid PGV-C which encodes luciferase showed a 2-fold increase in EGF receptor expressing cells, HEC-1-A, compared to that of EGF-non-labeled liposomes (N-liposomes). In EGF receptor deficient HRA cells, however, both EGF- and N-liposomes exhibited low transfection efficiency and no difference was observed between them. Furthermore, by the addition of anti EGF receptor antibody, transfection efficiency of EGF-liposomes was suppressed, suggesting EGF receptor-mediated endocytosis of EGF-liposomes. Transfection activity of EGF-liposomes was strongly dependent on the concentrations of fusogenic lipid, dioleoylphospha-tidylethanolamine in liposomes. By X-gal staining 6-8% of GCH-1(m) cells which also had EGF receptor expressed beta-galactosidase activity following the transfection by EGF-liposomes associating pSV-beta-galactosidase. These findings indicate that EGF-liposomes could be a preferable vector for EGF-receptor expressing cells.
authors.affiliation: Department of Obstetrics and Gynecology, Niiugata University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: S
authors.initials: S
authors.lastname: Sugaya
authors.forename: H
authors.initials: H
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Aramaki
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: H
authors.initials: H
authors.lastname: Arima
authors.forename: S
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: T
authors.initials: T
authors.lastname: Fuwa
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D002412:Cations; D002784:Cholesterol / Q000031:analogs & derivatives; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics*; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D010714:Phosphatidylethanolamines; D014407:Tumor Cells, Cultured
chemical_list: D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; D015060:1,2-Dipalmitoylphosphatidylcholine; D004815:Epidermal Growth Factor; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol; D066246:ErbB Receptors