Inhibition of chemiluminescence response of human mononuclear cells and suppression of mitogen-induced proliferation of spleen lymphocytes of mice by hispolon and hispidin.
The effects of two phenolic compounds, hispolon and hispidin isolated from fruit bodies of the basidiomycete Inonotus hispidus (Bull. ex Fr.) Karst, were investigated on the chemiluminescence response by LPS- or zymosan-activated human mononuclear cells (MNC) and on the concanavalin A-induced proliferation of spleen lymphocytes of mouse in vitro. Both compounds showed inhibitory activity in the chemiluminescence-test with an IC50 (the concentration of test compound causing 50% effect) ranging from 4.4 to 4.6 micrograms/ml (20.3 to 21.2 microM) for hispolon and < 0.1 to 1.5 micrograms/ml (from < 0.4 microM to 6.0 microM) for hispidin. Antiproliferative effects have been achieved in the lymphocyte transformation test (LTT) by hispolon with an IC50 of 3.4 micrograms/ml (15.5 microM).
Ali
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Institute of Pharmaceutical Biology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Lüdtke
J
J
Pilgrim
H
H
Lindequist
U
U
D000818:Animals; D001487:Basidiomycota / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D008934:Mitogens / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects*; D011753:Pyrones / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
D002396:Catechols; D008070:Lipopolysaccharides; D008934:Mitogens; D011753:Pyrones; C103442:hispolon; D003208:Concanavalin A; D015054:Zymosan; C007946:hispidin; D013936:Thymidine