Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis.
A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.
Matushek
M G
MG
Department of Internal Medicine, Rush Medical College, Chicago, Illinois 60612, USA.
Bonten
M J
MJ
Hayden
M K
MK
D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*
D004269:DNA, Bacterial