Neutrophil activation and chemotaxis after in vitro treatment with perfluorocarbon.
The in vitro effects of perfluorocarbon (PFC) on human neutrophil activation and chemotaxis were examined. Neutrophils were incubated, with and without PFC, and were analyzed for chemotaxis through 5-microns and 8-microns pore filters. Neutrophil activation was quantitated by flow cytometry. Activation studies showed that PFC-treated neutrophil samples (n = 6) produced only 39.83% +/- 25.9% (mean +/- SD) of matched control (n = 6) fluorescence. Chemotaxis studies showed that PFC-treated neutrophil samples (n = 8) had migration of only 18.63% +/- 6.5% (of control values) of neutrophils to the outer boundary of the 5-microns filters (n = 8 controls). The 8-microns pore filter migration (n = 8) was similarly low, with a mean outer boundary migration count of only 26% +/- 19.8% of the control (n = 8) value. Thus, neutrophils exposed to perfluorocarbon produce significantly less detectable H2O2 (P < .001) and have a significantly lower chemotactic response (P < .001).
Rossman
J E
JE
Department of Pediatric Surgery, Children's Hospital, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, NY, USA.
Caty
M G
MG
Rich
G A
GA
Karamanoukian
H L
HL
Azizkhan
R G
RG
D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005434:Flow Cytometry; D005466:Fluorocarbons / Q000009:adverse effects*; D006801:Humans; D006842:Hydrocarbons, Brominated; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D012121:Respiration, Artificial; D012128:Respiratory Distress Syndrome / Q000188:drug therapy
D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron