A new DNA marker, U15557, is linked to protease inhibitor and adenylate kinase-1 in the laboratory opossum, Monodelphis domestica.
A 323-bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. A HindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR, fragment as a hybridization probe. DNA samples exhibited either a 6.4 kb band, a 7.2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestica.
Perelygin
A A
AA
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78245-0549, USA.
Samollow
P B
PB
Perelygina
L M
LM
Cherry
L M
LM
Mahaney
S M
SM
VandeBerg
J L
JL
D000263:Adenylate Kinase / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009893:Opossums / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011480:Protease Inhibitors; D017422:Sequence Analysis, DNA; D012737:Sex Factors
D005819:Genetic Markers; D011480:Protease Inhibitors; D000263:Adenylate Kinase