Proton pump and auxin effect in Arabidopsis thaliana leaves during the development.
The plasma membrane from Arabidopsis thaliana leaves (wild-type and F mutant) has been purified by two-phase partitioning and the H(+)-transport activity was tested in vitro in the presence of different concentrations of IAA. While 1 microM of IAA had no effect, higher concentrations (10 microM and 100 microM) were inhibitory in 25 short days grown wild-type plants. However, the activity was increased by about 16% after 10 supplementary short days of growth (from 25 to 35 SD) in the represence of 10 microM of IAA. No significant sensitivity to the tested concentrations of auxin was observed during the development in short days (30, 38, 42 SD) or even the plants were induced by a 24 h of continuous light. The variability was not observed for a given developmental stage, from one experiment to another, but it was also observed after light induction of F mutant plants.
Bellamine
J
J
Laboratory of Plant Physiology and Biochemistry 3, place de l'Université CH, Geneva, Switzerland.
Greppin
H
H
D017360:Arabidopsis / Q000737:chemistry*; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D017494:Proton Pumps / Q000378:metabolism*
D007210:Indoleacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators; D017494:Proton Pumps; C030737:indoleacetic acid