Characterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing alpha(2-3)-linked sialic acid and alpha(1-3)-linked fucose. A sulphated variant of the VIM-2 epitope.
Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals) and digested with keratanase II, an endo- beta-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both alpha(2-3)-linked sialic acid and alpha(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Le(+) structure [NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-] was identified in this study. [equation: see text]
Brown
G M
GM
Division of Biological Sciences, University of Lancaster, U.K.
Huckerby
T N
TN
Abram
B L
BL
Nieduszynski
I A
IA
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D002358:Cartilage, Articular / Q000737:chemistry*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D000939:Epitopes / Q000737:chemistry*; D005643:Fucose; D007632:Keratan Sulfate / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000737:chemistry*
D000939:Epitopes; D009844:Oligosaccharides; C070946:VIM-2 determinant; D005643:Fucose; D007632:Keratan Sulfate; C086950:keratanase II; D000118:Acetylglucosaminidase; D019158:N-Acetylneuraminic Acid