title: In vitro activity of the trinem sanfetrinem (GV104326) against gram-positive organisms.
abstract: The in vitro activity of the trinem sanfetrinem (formerly GV104326) (GV) was compared with that of vancomycin, ampicillin, and/or nafcillin against 287 gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and multiresistant enterococci, by the agar and microbroth dilution methods. GV demonstrated 2 to 16 times more activity than ampicillin and nafcillin against the majority of these organisms. The MIC range of GV was 16 to 64 micrograms/ml for 19 Enterococcus faecium strains that were highly resistant to ampicillin (ampicillin MIC range, 64 to 512 micrograms/ml) and vancomycin resistant and 0.25 to 32 micrograms/ml for resistant Rhodococcus spp. Similar activities (+/-1 dilution) were observed by either the agar or the broth microdilution method. GV demonstrated bactericidal activity against a beta-lactamase-producing Enterococcus faecalis strain and against two methicillin-susceptible Staphylococcus aureus strains in 10(5)-CFU/ml inocula. Synergy between GV and gentamicin was observed against an E. faecalis strain that lacked high-level gentamicin resistance. The activity of GV suggests this compound warrants further study.
authors.affiliation: Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.
authors.forename: K V
authors.initials: KV
authors.lastname: Singh
authors.forename: T M
authors.initials: TM
authors.lastname: Coque
authors.forename: B E
authors.initials: BE
authors.lastname: Murray
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D004357:Drug Synergism; D013293:Enterococcus faecalis / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D007769:Lactams; D008826:Microbial Sensitivity Tests; D009254:Nafcillin / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; C086671:GV 104326; D005839:Gentamicins; D007769:Lactams; D010406:Penicillins; D009254:Nafcillin; D014640:Vancomycin; D000667:Ampicillin