title: Production of rotenone-inactivating substance(s) by rotenone-resistant insect cell line.
abstract: In a previous paper, we showed that a cell line derived from hemocytes of the cabbage armyworm, Mamestra brassicae (R-cell) was a thousand times as resistant to rotenone as that from ovaries of the same species (S-cell). The S-cells were killed by rotenone at concentrations higher than 10(-9) M, while R-cells at higher than 10(-6) M. When the R-cells were cultured in the medium containing 10(-9) M rotenone, the ability of rotenone to kill the S-cells was lost in the used medium. Also, when rotenone was incubated in the medium conditioned with R-cells, it lost its cell killing activity. It became evident that rotenone-inactivating substance(s) were produced in cells and stored in water-soluble form or liberated into the medium. The substance(s) were inactivated by heat treatment.
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagimoto
authors.forename: J
authors.initials: J
authors.lastname: Mitsuhashi
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D017077:Culture Media, Conditioned; D006361:Heating; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009036:Moths / Q000166:cytology*; D012402:Rotenone / Q000494:pharmacology*; D014867:Water
chemical_list: D017077:Culture Media, Conditioned; D012402:Rotenone; D014867:Water