Apolipoprotein B inhibits the hemolytic activity of asp-hemolysin from Aspergillus fumigatus.
We investigated the effect of human blood plasma lipoproteins on the hemolytic activity of Asp-hemolysin. Apolipoprotein B (apoB)-containing lipoproteins, such as low density lipoprotein (LDL) and very low density lipoprotein (VLDL), inhibit the activity of this hemolytic toxin. When Asp-hemolysin (15 mu g) was incubated with 100 mu g LDL (as protein), the hemolytic activity was inhibited by 90%. When 20 mu g apoB was added, the hemolytic activity was almost completely inhibited. Furthermore, similar inhibition was obtained in the filtrates which were separated from the incubation mixture of Asp-hemolysin with LDL or apoB following ultrafiltration through a membrane with a molecular cutoff = 100000. The current findings suggest that the inhibition by LDL is due to apoB binding to Asp-hemolysin.
Fukuchi
Y
Y
Pharmacy, Japanese Red Cross Sendai Hospital, Japan.
Kumagai
T
T
Ebina
K
K
Yokota
K
K
D001055:Apolipoproteins B / Q000378:metabolism / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology*; D006460:Hemolysin Proteins / Q000378:metabolism / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism; D011485:Protein Binding; D014462:Ultrafiltration
D001055:Apolipoproteins B; C089497:Asp-HS protein, Aspergillus fumigatus; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL