Regulation of mouse pancreatitis-associated protein-I gene expression during caerulein-induced acute pancreatitis.
The pancreatitis-associated proteins (PAPs) are major pancreatic secretory proteins during acute pancreatitis. However, mechanisms of regulation of PAP gene expression are poorly understood, and there is a lack of information regarding mouse PAP gene expression. Herein, we employed Northern blotting and RNase protection assays to measure mouse PAP-I mRNA levels in the normal pancreas and intestine, and in the pancreas during caerulein-induced acute pancreatitis. Unexpectedly, we found that mouse PAP-I mRNA levels are constitutively high in the adult pancreas, as well as in the small intestine. Furthermore, mouse pancreatic PAP-I mRNA levels are rapidly and dramatically down-regulated (3 h) after the initiation of caerulein injections, but slowly return to high levels by 72 h. Interestingly, we found that pancreatic PAP-I mRNA levels are also transiently and dramatically down-regulated after L-buthionine-[S,R]-sulfoximine administration. Thus, a correlation between PAP-I mRNA levels and glutathione levels in the mouse pancreas was demonstrated.
Fu
K
K
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160-7421, USA.
Sarras
M P
MP
De Lisle
R C
RC
Andrews
G K
GK
D000208:Acute Disease; D000209:Acute-Phase Proteins / Q000235:genetics*; D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002108:Ceruletide; D015536:Down-Regulation; D005786:Gene Expression Regulation; D005721:Glutamate-Cysteine Ligase / Q000037:antagonists & inhibitors; D005978:Glutathione / Q000032:analysis; D007421:Intestine, Small / Q000737:chemistry; D037181:Lectins, C-Type; D008297:Male; D051379:Mice; D010179:Pancreas / Q000737:chemistry*; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis
D000209:Acute-Phase Proteins; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; D012333:RNA, Messenger; C490253:Reg3b protein, mouse; D019328:Buthionine Sulfoximine; D002108:Ceruletide; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione