title: A simple procedure for large-scale purification of 9-cis beta-carotene from Dunaliella bardawil.
abstract: For experiments designed to obtain reliable data on the metabolic pathway and biological function of 9-cis beta-carotene, sufficient quantity of this substance in pure form is needed. For this purpose, we decided to purify 9-cis beta-carotene from the dry powder of the alga Dunaliella bardawil. By use of both silica gel and ODS open column chromatography and high-performance liquid chromatography (HPLC) on an ODS column and by treatment of the sample with ethanol, we could obtain this carotenoid as fine needle-shaped orange crystals. They were found to be highly pure as judged by analytical HPLC, absorption spectrum, and nuclear magnetic resonance analysis.
authors.affiliation: Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
authors.forename: N
authors.initials: N
authors.lastname: Ohishi
authors.forename: K
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000460:Chlorophyta / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D019207:beta Carotene / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D019207:beta Carotene