Development and validation of a capillary zone electrophoretic method for the determination of atenolol in presence of its related substances in bulk and tablet dosage form.
The development of a capillary zone electrophoresis method for determination of the drug atenolol in the presence of its related substances in bulk and in a tablet dosage form is described. The method was fully validated in terms of repeatability (RSDs for migration time and peak area of atenolol at 0.05 mg ml-1 were 0.25% and 0.52%, n = 10, respectively), reproducibility (RSD of peak area 0.84%, n = 5), linearity at two ranges of atenolol concentration, limits of detection and quantitation, ruggedness and robustness. The method was applied to the determination of the drug in a commercial tablet preparation (recovery 99.4%, m/m). The method proved to be fast and reliable for the quantitative analysis of atenolol in the presence of its related substances in bulk and pharmaceutical forms.
Shafaati
A
A
Pharmaceutical Chemistry, School of Pharmacy, University of Bradford, UK.
Clark
B J
BJ
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D001262:Atenolol / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D015203:Reproducibility of Results; D013607:Tablets / Q000032:analysis
D000319:Adrenergic beta-Antagonists; D013607:Tablets; D001262:Atenolol