Multiparameter monitoring and analysis of in vivo ischemic and hypoxic heart.
We describe a unique in vivo technique which addresses the multifactorial function of the heart, i.e., simultaneous measurement of myocardial ion transport (two mini-electrode systems to measure K+e and Ca2+e), energy metabolism (NADH fluorescence to measure NADH redox state), and coronary flow (laser-Doppler perfusion) using a multiprobe assembly (MPA) which contains transducers for all measurements. The MPA (which is 6 mm in diameter) was applied to the external surface of the heart in an open chest dog model. To test MPA function, myocardial ischemia was produced by application of a balloon occluder to the left anterior descending coronary (LAD) artery, and hypoxia was produced by changing the inspired O2-N2 ratio until the PaO2 was 20-30 torr. The MPA simultaneously monitored changes in ion flux, heart metabolism, and tissue perfusion during pathophysiological intervention.
Osbakken
M
M
Department of Biochemistry/Biophysics, University of Pennsylvania, Philadelphia, USA.
Mayevsky
A
A
D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism / Q000503:physiopathology; D003326:Coronary Circulation; D004195:Disease Models, Animal; D004285:Dogs; D004566:Electrodes; D005260:Female; D005336:Fiber Optic Technology; D005470:Fluorometry / Q000379:methods; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion
D009243:NAD