katG mutations in isoniazid-resistant Mycobacterium tuberculosis isolates recovered from Finnish patients.
katG and inhA genes from isoniazid-resistant Mycobacterium tuberculosis strains isolated in Finland were examined by PCR or sequencing. By PCR, katG was not detected in 3 of 54 strains. Sequencing of katG from 13 strains showed small point mutations or insertions; a previously described mutation causing a Ser-to-Thr change at position 315 was found in 4 strains, and there were nine new missense mutations of katG. A 209-bp segment of inhA from 17 strains was sequenced, but no mutations were observed. This result indicates that different mutations prevail in different geographical areas.
Marttila
H J
HJ
National Public Health Institute, Mycobacterial Reference Laboratory, Turku, Finland. harmar@utu.fi
Soini
H
H
Huovinen
P
P
Viljanen
M K
MK
D000995:Antitubercular Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005387:Finland; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D000995:Antitubercular Agents; D007538:Isoniazid