[A basic study on gastric emptying test using barium grains and effect of drugs on gastric emptying in rats].
We reported an experimental study on a new non-invasive method for evaluation of gastric emptying by abdominal X-ray after administration of radiopaque barium grains. Adult male Wistar rats weighing around 200 g were used. After they were fasted for 24 hours, 1 ml of gruel mixed with 10 barium grains (1 mm diameter) was introduced into the rat stomach with a catheter. The rats were sacrificed at 30, 60, 90, 120 and 150 minutes after the introduction of the gruel. X-rays were taken at each time point and grains in the stomach were counted in the X-ray photographs. All barium grains were emptied from the stomach in 150 minutes. After incision of the abdomen, the residual gastric contents were weighed. A positive correlation was found between the grains in the stomach and the weight of the contents. We studied the effects of cisapride, scopolamine buthylbromide and enprostil on the gastric emptying time by this method. Cisapride accelerated gastric emptying, whereas scopolamine buthylbromide delayed it. A prostaglandin E2 analog, enprostil delayed the gastric emptying. This method was found to be a simple procedure which is outstanding for quantitative determination and useful in evaluating gastric emptying functions.
Sakurai
T
T
First Department of Internal Medicine, Tokyo Jikei Medical University.
Torii
A
A
Tanaka
F
F
Asakawa
H
H
Matsuoka
M
M
Negishi
M
M
Kato
S
S
Hino
I
I
Inadama
E
E
Ariizumi
M
M
Toda
G
G
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001466:Barium Sulfate; D020117:Cisapride; D002523:Edible Grain; D016620:Enprostil / Q000494:pharmacology*; D005746:Gastric Emptying / Q000187:drug effects* / Q000502:physiology*; D005766:Gastrointestinal Contents; D008297:Male; D010276:Parasympatholytics / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology*; D011860:Radiography, Abdominal; D051381:Rats; D017208:Rats, Wistar
D000897:Anti-Ulcer Agents; D010276:Parasympatholytics; D010880:Piperidines; D001466:Barium Sulfate; D016620:Enprostil; D020117:Cisapride