A model for experimentally induced temperomandibular joint arthritis in rats: effects of carrageenan on neuropeptide-like immunoreactivity.
Substance P(SP)-, neurokinin A (NKA)-, calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-like immunoreactivity (-LI) was studied in rats' cerebrospinal fluid (CSF), plasma and perfusates (PF) from the temporomandibular joints (TMJs) at 2, 6 and 24 h following 0.01 ml injection of 2% carrageenan (CAR) into the right TMJ. SP-, NKA-, CGRP- and NPY-LI were significantly increased in both TMJ perfusates of the treated groups compared to controls. Generally an injection with CAR into the right TMJ induced a similar influence of the concentration of SP-, NKA-, CGRP- and NPY-LI in the CSF, plasma and PF at 2, 6 and 24 h following injection. However, the most pronounced changes in neuropeptide-LI occurred intra-articularly in the joint fluid, which indicates that both the sensory and sympathetic nervous system are activated in this joint following osteoarthritis induction by carrageenan.
Lundeberg
T
T
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Alstergren
P
P
Appelgren
A
A
Appelgren
B
B
Carleson
J
J
Kopp
S
S
Theodorsson
E
E
D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology; D002351:Carrageenan; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013582:Synovial Fluid / Q000378:metabolism; D013704:Temporomandibular Joint / Q000378:metabolism* / Q000473:pathology
D009479:Neuropeptides; D002351:Carrageenan