Immunohistochemical analysis of extracellular matrix components and cytoskeletal products of bone marrow stromal cells.
We analysed the cytoskeletal proteins and extracellular matrix components of in vitro cultured BMSC both in resting state and after activation with IFN gamma and TNF alpha, using an immunoperoxidase procedure. BMSC expressed fibronectin, alpha-actin, beta-tubulin, vimentin and vinculin while cytokeratinpan, GFAP, neurofilament, desmin and laminin were not expressed. This pattern of expression was not affected by addition of TNF alpha and IFN gamma, but differs from human tonsil stromal cells for laminin expression and alpha-actin localization.
Lisignoli
G
G
Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, IOR, Bologna.
Toneguzzi
S
S
Monaco
M C
MC
Bertollini
V
V
Facchini
A
A
D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003238:Connective Tissue / Q000737:chemistry / Q000187:drug effects; D003239:Connective Tissue Cells; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D003599:Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007797:Laminin / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D014066:Palatine Tonsil / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D007797:Laminin; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma