title: Development of a two-site immunoassay of recombinant urate oxidase (SR 29142) and its use for determination of pharmacokinetic parameters in rats and baboons.
abstract: Two monoclonal antibodies (Mabs), 12C7 and 11G11, both directed against recombinant urate oxidase (SR 29142), were selected for their epitope specificity to develop a two-site immunoassay of urate oxidase in plasma. A quantitative recovery of urate oxidase in plasma was obtained at all the concentrations tested, and the limit of quantification was found to be 0.5 ng/mL. Intra-and interassay coefficients of variation ranged from 1.2 to 6.7% and from 3.5 to 10.8%, respectively. The specificity of the two antibodies was studied in Western-blot experiments. This assay was used successfully to determine urate oxidase pharmacokinetic parameters after intravenous injection in rats and baboons. In these two species, urate oxidase pharmacokinetics was characterized by a low clearance and a low volume of distribution without gender difference.
authors.affiliation: Sanofi Recherche, Montpellier, France.
authors.forename: D
authors.initials: D
authors.lastname: Dussossoy
authors.forename: M L
authors.initials: ML
authors.lastname: Py
authors.forename: G
authors.initials: G
authors.lastname: Pastor
authors.forename: X
authors.initials: X
authors.lastname: Boulenc
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D007118:Immunoassay; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010215:Papio; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014503:Urate Oxidase / Q000032:analysis / Q000276:immunology / Q000493:pharmacokinetics*
chemical_list: D000911:Antibodies, Monoclonal; D014503:Urate Oxidase