title: Anti-IRS-1 monoclonal antibody, 6G5, cross-reacts to IRS-2.
abstract: We previously reported the production of anti-IRS-1 monoclonal antibodies against human IRS-1 C-terminal portion. One of anti IRS-1 monoclonal antibodies, 6G5, was found to immunologically cross-react with IRS-2, which has been reported recently. The data presented here provide information arising from the use of the anti-IRS-1 MAb, 6G5; this MAb was found to be a useful reagent in studying the functional role of IRSs in the insulin-signaling system.
authors.affiliation: Department of Biochemistry II, Daisan Hospital, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamauchi
authors.forename: H
authors.initials: H
authors.lastname: Nishimaki
authors.forename: A
authors.initials: A
authors.lastname: Furusaka
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Tamemoto
authors.forename: S
authors.initials: S
authors.lastname: Taylar
authors.forename: K
authors.initials: K
authors.lastname: Tobe
authors.forename: T
authors.initials: T
authors.lastname: Kadowaki
authors.forename: M
authors.initials: M
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases; D010748:Phosphopeptides / Q000276:immunology; D010750:Phosphoproteins / Q000172:deficiency / Q000276:immunology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C526292:IRS1 protein, human; C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; C526295:Irs1 protein, mouse; C526298:Irs2 protein, mouse; D010748:Phosphopeptides; D010750:Phosphoproteins; D011994:Recombinant Proteins; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor)