The product of the v-src-inducible gene nr-13 is a potent anti-apoptotic factor.
Tumorigenesis can be induced either by activating cell proliferation or by inhibiting metabolic pathways regulating programmed cell death (apoptosis). There is evidence suggesting that p60(v-src) and other tyrosine kinases protect cells against apoptosis. This effect could contribute to cell transformation by the Rous sarcoma virus. Mechanism of cell death inhibition by p60(v-src) remains largely unknown. We have recently reported that in avian cells p60(v-src) activates the expression of nr-13, a bcl-2-related gene. In this paper, we demonstrate, using the bone marrow derived cell line Baf-3 as an experimental model, that the product of this avian gene (nr-13) is a potent anti-apoptotic factor. In addition, we report that, in quail neuroretinal cells, nr-13 expression is activated upon infection by the Rous sarcoma virus (RSV) but not by other oncogenic retroviruses like FSV or MH2, suggesting that nr-13 is a specific target of v-src. Activation of nr-13 expression may be a key step in cellular transformation by v-src.
Mangeney
M
M
Immunologie Cellulaine, Ecole normale supérieure de Lyon, France.
Schmitt
J R
JR
Leverrier
Y
Y
Thomas
J
J
Marvel
J
J
Brun
G
G
Gillet
G
G
D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D030161:Avian Proteins; D001358:Avian Sarcoma Viruses; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D003370:Coturnix; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007377:Interleukin-3 / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D015688:Oncogene Protein pp60(v-src) / Q000502:physiology
D030161:Avian Proteins; D004273:DNA, Neoplasm; D007377:Interleukin-3; D008565:Membrane Proteins; C093264:NR13 protein, avian; D015688:Oncogene Protein pp60(v-src)