Cloning and high expression of rabbit FKBP25 in cornea.
Rabbit 25 kDa FK-506/rapamycin binding protein (FKBP25) cDNA was isolated during a screening of rabbit corneal endothelial cDNA library. The cDNA clone was a 955 bp fragment with a 670 bp open reading frame and a 285 bp 3' non-coding region. The cDNA and deduced amino acid sequences were 91.7 and 96.4% identical, respectively, to previously reported human FKBP25B sequences. The lysine-rich region KK(X)7KK(X)26KKKK, which plays an important role in nuclear translocation in rabbit FKBP25, was recognized. Reverse transcription-polymerase chain reaction (RT-PCR) amplification of rabbit FKBP25 and FKBP12 in selected rabbit organs, including total cornea, showed higher expressions in cornea, retina, cerebrum and cerebellum than other compared tissues. RT-PCR amplification in rabbit cultured corneal cells showed expressions in all cultured corneal epithelial, stromal, endothelial, and especially in SV40-adenovirus vector-immortalized epithelial cells. The higher expressions of FKBP25 and FKBP12 suggest significant roles for FKBPs in those tissues.
Kitagawa
H
H
Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
Hotta
Y
Y
Fujiki
K
K
Kanai
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D003315:Cornea / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004848:Epithelium / Q000378:metabolism / Q000821:virology; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D012160:Retina / Q000378:metabolism; D016559:Tacrolimus / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins; D014158:Transcription, Genetic
D002352:Carrier Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004247:DNA; D022021:Tacrolimus Binding Proteins; D016559:Tacrolimus