ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor.
To identify the functional P2 purinoceptor subtype in glomerular mesangial cells (GMC), polymerase chain reaction coupled with reverse transcription (RT-PCR) were performed, and cytosolic free calcium concentration ([Ca2+]i) was determined. RT-PCR analysis revealed that the molecular identity of P2 purinoceptor localized to GMC was both P2U and P2Y. The rank order of potency in stimulating [Ca2+]i was ATP [symbol: see text] UTP > 2-methylthio-ATP > ADP [symbol: see text] adenosine [symbol: see text] AMP. In addition, cross-desensitization between ATP and UTP occurred. In conclusion, ATP induces increase in [Ca2+]i via P2U purinoceptor in GMC.
Takeda
M
M
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Kawamura
T
T
Kobayashi
M
M
Endou
H
H
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D005920:Glomerular Mesangium / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism*; D058473:Receptors, Purinergic P2Y2; D014158:Transcription, Genetic; D014544:Uridine Triphosphate / Q000494:pharmacology
D018076:DNA, Complementary; C546386:P2ry2 protein, mouse; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate