Inhibitory effects of sterols isolated from Chlorella vulgaris on 12-0-tetradecanoylphorbol-13-acetate-induced inflammation and tumor promotion in mouse skin.
Inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice was observed in the methanol extract of Chlorella vulgaris, a green alga. The hexane soluble fraction obtained from the methanol extract exhibited marked inhibitory activity from which were isolated two delta(5,7)-sterols (ergosterol and 7-dehydroporiferasterol), two delta(5,7,9(11))-sterols [7,9(11)-dehydroergosterol and 7,9(11)-bisdehydroporiferasterol], two 5 alpha, 8 alpha-epidioxy-delta(6)-sterols (ergosterol peroxide and 7-dehydroporiferasterol peroxide), and a 7-oxo-delta(5)-sterol (7-oxocholesterol), among others. The delta 5'7-sterols, 5 alpha, 8 alpha-epidioxy-delta(6)-sterols and 7-oxo-delta 5-sterol inhibited TPA-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 0.2-0.7 mg/ear. Furthermore, ergosterol peroxide markedly inhibited the tumor-promoting effect of TPA in 7,12-dimethylbenz[a]anthracene-initiated mice.
Yasukawa
K
K
College of Pharmacy, Nihon University, Japan.
Akihisa
T
T
Kanno
H
H
Kaminaga
T
T
Izumida
M
M
Sakoh
T
T
Tamura
T
T
Takido
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D002708:Chlorella / Q000737:chemistry*; D002845:Chromatography; D004875:Ergosterol / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D012867:Skin / Q000187:drug effects*; D012871:Skin Diseases / Q000139:chemically induced / Q000517:prevention & control; D013261:Sterols / Q000737:chemistry / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D002457:Cell Extracts; D013261:Sterols; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol