Fab fragments of the monoclonal antibody BCF2 are capable of neutralizing the whole soluble venom from the scorpion Centruroides noxius Hoffmann.
BCF2 is a murine hybridoma cell line that produces a neutralizing antibody against toxin 2 (Cn2) from the scorpion Centruroides noxius Hoffmann of Mexico. In this communication we report the preparation and use of the BCF2 antibody and its antigen binding fragments (Fab) in experiments aiming at obtaining protection of experimental albino mice (strain CD1) challenged with purified toxin Cn2, as well as, with whole soluble venom from C. noxius. The monoclonal antibody BCF2 in amounts of 1 mg neutralizes 28 LD50 of soluble venom of C. noxius, whereas the Fab fragments of BCF2 (1 mg) are capable of neutralizing 43 LD50 dose of the same venom. To reach the same level of neutralization, with the commercially available horse antiserum [F(ab')2], we need to use about ninefold more material.
Licea
A F
AF
Department of Molecular Recognition and Structural Biology, National Autonomous University of Mexico, Cuernavaca, Mexico.
Becerril
B
B
Possani
L D
LD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007928:Lethal Dose 50; D051379:Mice; D009500:Neutralization Tests; D012604:Scorpion Venoms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D012604:Scorpion Venoms