Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43.
Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the gaIETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a gaIU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase.
Chang
H Y
HY
Department of Molecular and Cellular Biology, Chang Gung College of Medicine and Technology, Kwei-San, Taiwan.
Lee
J H
JH
Deng
W L
WL
Fu
T F
TF
Peng
H L
HL
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D016296:Mutagenesis; D009154:Mutation; D014774:Virulence / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins