Kinetic of in vitro generation of some hemorphins: early release of LVV-hemorphin-7, precursor of VV-hemorphin-7.
Bovine globin has been hydrolysed by pepsin to different degrees of hydrolysis. Analysis of the hydrolysates, by reversed-phase high-performance liquid chromatography (RP-HPLC), shows the release of LVV- and VV-hemorphin-7. LVV-hemorphin-7 was the first generated, at a degree of hydrolysis (DH), as low as 4%. In contrast, VV-hemorphin-7 was produced later. Our study clearly shows that VV-hemorphin-7 is issued directly from LVV-hemorphin-7, since this later completely disappeared during hydrolysis. This work allows us to suggest a possible pathway for in vivo hemorphins appearance.
Dagouassat
N
N
Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences et Technologies, La Rochelle, France.
Garreau
I
I
Zhao
Q
Q
Sannier
F
F
Piot
J M
JM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005914:Globins / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D006454:Hemoglobins; D010446:Peptide Fragments; C074309:LVV-hemorphin-7; C094394:VV-hemorphin-7; D005914:Globins