Studies on the transfer techniques of three maize genes.
Maize transformation has been carried out through microprojectile bombardment, ultrasonication in a DNA buffer, and ovary-injection with a self-made microinjector. The plasmid pB48.415, which carries a 3'-truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with a particle gun, embryogenic calli ultrasonicated under different conditions of ovaries injected 10-20 hours after pollination. The results of Dot blotting and Southern blotting analyses proved the integration of the Bt gene into maize genome.
Wang
G
G
College of Biology, Beijing Agricultural University, China.
Zhang
H
H
Ding
Q
Q
Xie
Y
Y
Dai
J
J
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D046508:Culture Techniques; D004731:Endotoxins / Q000235:genetics; D018014:Gene Transfer Techniques; D017343:Genes, Plant; D006460:Hemolysin Proteins; D007267:Injections; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic; D014465:Ultrasonics; D003313:Zea mays / Q000235:genetics*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase