Sub-typing of animal and human Campylobacter spp. using RAPD.
Based on a 10-mer primer (5'-CCTGTTAGCC-3'), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. The method was also successfully applied to typing Campylobacter jejuni from a similar range of sources.
Madden
R H
RH
Food Science Department, Department of Agriculture for Northern Ireland, Belfast, UK. R.Madden@QUB.AC.UK
Moran
L
L
Scates
P
P
D000818:Animals; D002167:Campylobacter / Q000145:classification* / Q000235:genetics; D002169:Campylobacter Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013552:Swine / Q000382:microbiology
D004269:DNA, Bacterial