The SCID but not the RAG-2 gene product is required for S mu-S epsilon heavy chain class switching.
We have investigated the capacity of precursor B cells from normal (BDF1) and V(D)J recombinase-deficient (RAG-27) or defective (SCID) mice to be induced by a CD40-specific monoclonal antibody and IL-4 to epsilon H chain gene transcription and to S mu-S epsilon switch recombination. In differentiating precursor B cells from all three strains of mice, the development of similar numbers of CD19+, CD23+, CD40+, and MHC class II+ expressing B lineage cells and similar levels of epsilon H chain gene transcription were induced. Efficient S mu-S epsilon switching occurred in normal and RAG-2-deficient, but not in SCID, precursor B cells. Thus, the transcription of the epsilon H chain is independent of the RAG-2 and the SCID gene product, while the S mu-S epsilon switch recombination requires the SCID gene-encoded DNA-dependent protein kinase, but not the RAG-2 protein.
Rolink
A
A
Basel Institute for Immunology, Switzerland.
Melchers
F
F
Andersson
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology*; D019013:CD40 Antigens / Q000502:physiology; D002454:Cell Differentiation; D004268:DNA-Binding Proteins; D018507:Gene Expression Regulation, Developmental; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000494:pharmacology; D015851:Interleukin-7 / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016513:Mice, SCID / Q000276:immunology*; D011506:Proteins / Q000502:physiology*; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D004268:DNA-Binding Proteins; D007139:Immunoglobulin epsilon-Chains; D007148:Immunoglobulin mu-Chains; D015851:Interleukin-7; D011506:Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; D015847:Interleukin-4