CFTR expression in C127 cells is associated with enhanced cell shrinkage and ATP extrusion in Cl(-)-free medium.
In this study we have employed three lines of C127 murine cells. C127 CFTR w/t, C127 CFTR delta F508 and C127 mock, transfected with, respectively, wild type, delta F508 mutant human CFTR cDNA or the vector only. In the first 10 minutes of a Cl(-)-free incubation the three cell lines exhibit a significant shrinkage due to a loss of K+ and Cl-. However, C127 CFTR w/t cells shrink more than C127 CFTR delta F508 and the mock cells. The supplementation of Cl(-)-free medium with ATP causes a marked decrease in the cell volume of C127 CFTR delta F508 and of the mock cells but not of C127 CFTR w/t cells. ATP effect is mimicked by adenosine 5'-O-(3-thiotriphosphate), but neither by adenosine nor by UTP. Measurements of extracellular ATP indicate that during the Cl(-)-free incubation C127 CFTR w/t cells extrude more ATP than the other two cell lines. The results are consistent with the hypothesis that CFTR enhances K+ and Cl- permeabilities by promoting the extrusion of ATP.
Rotoli
B M
BM
Istituto di Patologia Generale, Università degli Studi di Parma, Italy.
Bussolati
O
O
Dall' Asta
V
V
Hoffmann
E K
EK
Cabrini
G
G
Gazzola
G C
GC
D000255:Adenosine Triphosphate / Q000378:metabolism*; D048429:Cell Size; D002712:Chlorides / Q000378:metabolism*; D003001:Cloning, Molecular; D003470:Culture Media; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D018076:DNA, Complementary; D006801:Humans; D009154:Mutation; D009994:Osmolar Concentration; D014407:Tumor Cells, Cultured
C505032:CFTR protein, human; D002712:Chlorides; D003470:Culture Media; D018076:DNA, Complementary; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000255:Adenosine Triphosphate