title: Anergy induction in human CD4+ T-cell clones by stimulation with soluble peptides does not require cell proliferation and is accompanied by elevated IL4 production.
abstract: The stimulation of activated T cells with soluble peptides or peptide-pulsed T-APC in the absence of professional APC can anergize peptide-specific T cells. Here, we studied human T cell clones (TCCs) that either proliferate (T-responder) or do not proliferate (T-nonresponder) to activated T cells as antigen-presenting cells (APC) and investigated the efficacy of anergy induction in these two types of TCCs. The TCCs were specific to the p30 peptide from tetanus toxoid and secreted either a Th0- or a Th1-like cytokine pattern. To induce anergy, the TCCs were first stimulated by addition of the peptides directly to the cell cultures without additional APC (T-APC). Anergy was detected by restimulating these TCCs on professional B-APC. The proliferation, production of cytokines (IL2, IFN-gamma, IL4, IL5, IL10), and the cytotoxicity were measured after the first and second stimulation and compared with nonanergized control cells. Priming of TCCs by T-APC (anergy induction) resulted in an elevated production of IL4. This cytokine shift was also seen in the T-nonresponder TCC despite no induced proliferation. Th1-like TCCs retained their cytotoxicity after anergy induction. In contrast to cells first activated by B-APC, the restimulation of TCCs primed by T-APC lead to a drastic reduction of proliferation and cytokine production for both T-responder and T-nonresponder TCCs. The functional down-regulation of TCCs mediated by soluble peptides could be overcome by addition of IL2, but not by IL1 or IL4. We concluded that the induction of T-cell anergy does not require cell proliferation.
authors.affiliation: Institute of Immunology and Allergology, Inselspital, Bern, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Grunow
authors.forename: K
authors.initials: K
authors.lastname: Frutig
authors.forename: W J
authors.initials: WJ
authors.lastname: Pichler
mesh_terms: D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division; D017635:Clonal Anergy / Q000276:immunology*; D002999:Clone Cells; D016207:Cytokines / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D013744:Tetanus Toxin / Q000276:immunology*
chemical_list: D000941:Antigens; D016207:Cytokines; C091828:P30 tetanus toxin peptide; D010446:Peptide Fragments; D013744:Tetanus Toxin; D015847:Interleukin-4