title: A linkage map of the ovine X chromosome.
abstract: A genetic linkage map of the ovine X chromosome containing type I and type II markers has been constructed. The map contains 7 known gene markers and 14 microsatellite markers with a recombination length of 141.9 cM. Segregation of polymorphic markers was observed in a three-generation pedigree containing 480 animals. The maximum number of informative meioses was 912. Additional information was obtained for some markers by following segregation in the AgResearch International Mapping Flock, consisting of nine three-generation full-sib pedigrees. A pseudoautosomal region containing two markers has been identified at one end of the linkage map. Comparisons with mouse and human X chromosomes confirms the observation of Ohno (1973) that the gene content of the mammalian X chromosome is retained. In particular, the conserved grouping of the genes PHKA1, ATP7A, and XIST observed in both the human and the mouse X chromosome appears to be conserved in the sheep X chromosome, and XIST has been mapped to near the center of the chromosome. This study provides the first reported genetic linkage map combining both type I and type II markers for any ruminant X chromosome.
authors.affiliation: AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago, Dunedin, New Zealand. galloways@agresearch.cri.nz
authors.forename: S M
authors.initials: SM
authors.lastname: Galloway
authors.forename: V
authors.initials: V
authors.lastname: Hanrahan
authors.forename: K G
authors.initials: KG
authors.lastname: Dodds
authors.forename: M D
authors.initials: MD
authors.lastname: Potts
authors.forename: A M
authors.initials: AM
authors.lastname: Crawford
authors.forename: D F
authors.initials: DF
authors.lastname: Hill
mesh_terms: D000818:Animals; D015415:Biomarkers; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012756:Sheep / Q000235:genetics*; D014960:X Chromosome
chemical_list: D015415:Biomarkers