Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis.
Analyses of a series of allelic chlorina mutants of wheat (Triticum aestivum L.), which have partial blocks in chlorophyll (Chl) synthesis and, therefore, a limited Chl supply, reinforce the principle that Chl is required for the stable accumulation of Chl-binding proteins and that only reaction centers accumulate when the supply of Chl is severely limited. Depending on the rate of Chl accumulation (determined by the severity of the mutation) and on the rate of turnover of Chl and its precursors (determined by the environment in which the plant is grown), the mutants each reach an equilibrium of Chl synthesis and degradation. Together these mutants generate a spectrum of phenotypes. Under the harshest conditions (high illumination), plants with moderate blocks in Chl synthesis have membranes with very little Chl and Chl-proteins and membrane stacks resembling the thylakoids of the lethal xantha mutants of barely grown at low to medium light intensities (which have more severe blocks). In contrast, when grown under low-light conditions the same plants with moderate blocks have thylakoids resembling those of the wild type. The wide range of phenotypes of Chl b-deficient mutants has historically produced more confusion than enlightenment, but incomparable growth conditions can now explain the discrepancies reported in the literature.
Falbel
T G
TG
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309, USA. tgfalbel@facstaff.wisc.edu
Meehl
J B
JB
Staehelin
L A
LA
D002734:Chlorophyll / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics*; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000528:radiation effects / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D007425:Intracellular Membranes / Q000528:radiation effects / Q000648:ultrastructure; D008027:Light; D045342:Light-Harvesting Protein Complexes; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis; D011524:Protoporphyrins / Q000378:metabolism; D013045:Species Specificity; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D011524:Protoporphyrins; D002734:Chlorophyll; C037184:chlorophyll b; C028025:protoporphyrin IX; D000077194:Chlorophyll A