Interactions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis.
Using the Southwestern blot analysis we have studied the interactions between rat repetitive sequence MspI8 and the nuclear matrix proteins of rat testis cells. Starting from 2 weeks the young to adult animals showed differences in type of testis nuclear matrix proteins recognizing the MspI8 sequence. The same sets of nuclear matrix proteins were detected in some fractions enriched in spermatocytes and spermatides and obtained after fractionation of testis cells of adult animals by the velocity sedimentation technique.
Rogoliński
J
J
Institute of Oncology, Department of Tumor Biology, Gliwice, Poland.
Widłak
P
P
Rzeszowska-Wolny
J
J
D000818:Animals; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000378:metabolism*; D015415:Biomarkers; D015139:Blotting, Southern; D015153:Blotting, Western; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism
D034961:Antigens, Nuclear; D001324:Autoantigens; D015415:Biomarkers; D009687:Nuclear Proteins