The effect of a synthetic analogue of prostaglandin E2 on wound healing in rats.
This was a prospective, randomized, double-blind experimental study to assess the effect of a long-acting prostaglandin (PG) analogue on wound healing in rats. Eight-cm long, dorsal midline skin incisions were made on rats (n = 44) and immediately resutured. The treatment group (n = 22) received daily intraperitoneal injections of 16,16-dimethyl prostaglandin E2-methyl ester (di-MPGE2) for 7 days, whereas the controls (n = 22) received normal saline. The rats were sacrificed in groups of 11 at 7 and 14 days, respectively. The wounds were excised and analyzed. Histology showed that there was increased fibrosis (p < 0.03) and a decreased number of macrophages (p < 0.02) in the PG group at 7 days. Tensile strength and hydroxyproline content also increased but did not attain significant levels. The differences between the PG and control groups at 14 days similarly did not attain statistical significance. The results suggest that in wound healing, the administration of di-MPGE2 may be beneficial during the early stages of inflammation, rather than during the later stages of remodelling.
Talwar
M
M
Department of Surgery, Royal University Hospital, Saskatoon, Saskatchewan, Canada.
Moyana
T N
TN
Bharadwaj
B
B
Tan
L K
LK
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D015232:Dinoprostone / Q000031:analogs & derivatives*; D005355:Fibrosis; D006097:Granulation Tissue / Q000473:pathology; D006909:Hydroxyproline / Q000378:metabolism; D008264:Macrophages / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000473:pathology / Q000503:physiopathology
D015232:Dinoprostone; D015064:16,16-Dimethylprostaglandin E2; D006909:Hydroxyproline