Pharmacological modulations of adrenergic phenotype in medullary C2 and C3 cell groups of adult rat.
The adrenergic phenotype was analysed in the rat's rostral dorsomedial medulla under normal conditions and 3 days after a single intraperitoneal injection of an eburnamine derivative, RU 24722, which increases tyrosine hydroxylase protein expression in the rostral portion of the nucleus tractus solitarius. This approach was investigated by a double immunofluorescence labelling of tyrosine hydroxylase and phenylethanolamine N-methyltransferase proteins. Under normal conditions, most adrenergic cell bodies are anatomically distributed in the dorsal and rostral medulla oblongata between the rostral part of the dorsal motor nucleus of the vagus nerve and the medial longitudinal fasciculus. Adrenergic neurons detected in this medullar region were distributed between both cell groups. Three days after the pharmacological RU 24722 treatment, an upregulation in tyrosine hydroxylase and phenylethanolamine N-methyltransferase protein expression was detected in both cell groups characterized by a highly increased number of tyrosine hydroxylase- and phenylethanolamine N-methyltransferase-containing cell bodies. The number of TH-mRNA containing neurons was also increased, indicating the transcriptional level of this regulation. These results demonstrated a particular neuronal plasticity of adrenergic phenotype in the medullary cell groups of adult rat.
Garcia
C
C
Laboratoire de Neuropharmacologie Moléculaire, C.N.R.S.-U.M.R. 5542, Faculté de Médecine, Lyon, France.
Denoroy
L
L
Le Cavorsin
M
M
Pujol
J F
JF
Weissmann
D
D
D000704:Analysis of Variance; D000818:Animals; D004353:Drug Evaluation, Preclinical; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000737:chemistry*; D010641:Phenotype; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013564:Sympathetic Nervous System / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D014749:Vincamine / Q000031:analogs & derivatives* / Q000494:pharmacology
D014749:Vincamine; C044616:vindeburnol; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase