title: [Damage of intestinal mucosal barrier function after fluid resuscitation during hemorrhagic shock].
abstract: The aim of this study is to evaluate the effects of fluid resuscitation during hemorrhagic shock on bacterial overgrowth, bacterial translocation and intestinal pathology. Thirty male Sprague-Dawley rats were divided into three groups as follows: sham shock (anesthesia and femoral artery catheterization, control) was performed on group C, on group B, blood was withdrawn and maintained at a mean blood pressure of 25 mmHg for 30 min, followed by resuscitation with whole shed blood; and on group L, shock was induced by the same method, followed by resuscitation with the same shed blood volume of lactated Ringer's solution. Rats were sacrificed at 24h postshock, and bacterial cultures of blood, liver, mesenteric lymph node and cecum were obtained for standard bacteriological examinations. Furthermore, histologic examination of the end of the ileum was performed. The results indicate the pathologic mucosal injury, bacterial overgrowth and the high magnitudes of translocation occurred more frequently in group L than in groups C and B. Thus, resuscitation with lactated Ringer's solution alone for hemorrhagic shock induces intestinal histologic damage and bacterial overgrowth and leads to increase bacterial translocation.
authors.affiliation: Department of Anesthesiology, Fukushima Medical College.
authors.forename: J
authors.initials: J
authors.lastname: Shimada
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D005440:Fluid Therapy; D007413:Intestinal Mucosa / Q000473:pathology*; D007552:Isotonic Solutions / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D013210:Staphylococcus / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; D000077325:Ringer's Lactate