Effect of pyloric cuffs on cholecystokinin satiety.
Because inflation of a pyloric cuff during feeding blocks gastric emptying and increases gastric distention, this device was used to test the hypothesis of synergism between gastric distention and cholecystokinin octapeptide (CCK-8) in reducing intake. Adult, male Sprague-Dawley rat were allowed 20-min access to 30% sucrose, with or without inflation of pyloric cuffs, after intraperitoneal injection of saline or 1, 2, or 4 micrograms/kg CCK-8. Cuff inflation resulted in large increases in gastric volume at the end of feeding tests. However, this manipulation failed to enhance the effectiveness of any dose of CCK-8. In fact, data analyses revealed trends suggestive of decreased effectiveness of the two lowest doses under these conditions. The effect of 4 micrograms/kg CCK-8 was almost identical in tests with and without cuff inflation. These results are contrary to the prediction of greater potency of CCK-8 resulting from increased gastric distention.
Cox
J E
JE
Department of Psychology, University of Alabama, Birmingham 35294, USA.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D008297:Male; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012528:Satiety Response / Q000187:drug effects*; D012844:Sincalide / Q000494:pharmacology*; D013270:Stomach / Q000187:drug effects*; D013395:Sucrose / Q000378:metabolism
D013395:Sucrose; D012844:Sincalide