Luteinizing hormone-releasing hormone in undifferentiated and differentiated SK-N-SH human neuroblastoma cells.
The presence of luteinizing hormone-releasing hormone (LHRH) in SK-N-SH human neuroblastoma cells was investigated by immunocytochemistry and enzyme-linked immunosorbent assays of whole cell extracts and culture medium. In addition, ribonuclease protection assays were utilized to quantitate LHRH messenger RNA. The expression of LHRH mRNA and LHRH protein level was correlated with neuronal differentiation induced by retinoic acid (RA). In differentiated SK-N-SH cells the LHRH mRNA level as well as the amount of LHRH in cell extracts and cell medium were significantly lower than in differentiated cells. These results suggest that RA affects the expression of LHRH mRNA and the level of LHRH protein in SK-N-SH cells. These data show that altering the growth state of the human neuroblastoma SK-N-SH cells toward more neuronal phenotype results in a significant decrease in expression of LHRH mRNA and the protein. The ability of RA to induce changes in LHRH at the mRNA and at the peptide levels will allow further study of RA regulation of LHRH at the neuronal level.
Ratka
A
A
Department of Pharmaceutical Sciences, Idaho State University, Pocatello 83209, USA.
Flores
B M
BM
Mambourg
S E
SE
Torian
B E
BE
D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D002454:Cell Differentiation; D015342:DNA Probes; D018076:DNA, Complementary / Q000096:biosynthesis; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense; D010641:Phenotype; D015347:RNA Probes; D012260:Ribonucleases / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D015347:RNA Probes; D007987:Gonadotropin-Releasing Hormone; D014212:Tretinoin; D012260:Ribonucleases