Retrograde fluorescent double-labeling study of bilaterally projecting retinal ganglion cells in albino rats at different stages of development.
Injection of the fluorescent tracers 10% Evans blue (EB) and 4% fluoro-gold (FG) into the right and the left dorsal lateral geniculate nucleus, respectively, of albino rats at different stages of development demonstrated the presence of double-labeled retinal ganglion cells that projected bilaterally into both the dorsal lateral geniculate nuclei (dLGN). Findings confirmed that the distribution of these double-labeled cells was gradually reduced after birth, being confined to the peripheral temporoventral quarter (temporal-ventral crescent) of the retina after postnatal day 15. We estimated the proportion of double-labeled cells to total labeled cells in the same area at different stages of development (0-90 days); values ranged from 35.3% in the neonate to 5.27% in the adult rat which suggests that the majority of double-labeled cells and/or their axons were lost early in development. That a small number of ganglion cells were observed to project bilaterally in the adult rats suggested that these cells conduct the same visual information to both hemispheres throughout the animal's life.
Dong
K
K
First Department of Anatomy, Kobe University School of Medicine, Japan.
Ahmed
A K
AK
Qu
T
T
Sugioka
K
K
Yamada
K
K
Yamadori
T
T
D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D005070:Evans Blue; D005456:Fluorescent Dyes; D005829:Geniculate Bodies / Q000166:cytology / Q000254:growth & development*; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000254:growth & development*; D012165:Retinal Ganglion Cells / Q000502:physiology*; D013266:Stilbamidines; D014795:Visual Pathways / Q000166:cytology / Q000502:physiology
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D005070:Evans Blue