Immunization of mice with recombinant L7/L12 ribosomal protein confers protection against Brucella abortus infection.
BALB/c mice were immunized with the recombinant Brucella abortus L7/L12 ribosomal protein fused to maltose binding protein (MBP). Vaccinated animals mounted a specific immune response to the recombinant fusion protein as demonstrated by immunoblot analyses. Additionally, B. abortus L7/L12 ribosomal protein conferred a significant degree of protection when compared to mice vaccinated with adjuvant alone, adjuvant plus MBP or B. abortus. These results indicate that a recombinant B. abortus protein, previously identified as T-cell-reactive, engendered protective immunity to mice against brucellosis.
Oliveira
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SC
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
Splitter
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GA
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D002003:Brucella abortus / Q000302:isolation & purification; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D012269:Ribosomal Proteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D012269:Ribosomal Proteins; C512845:Rpl7 protein, mouse; D014614:Vaccines, Synthetic; C016700:ribosomal protein L7-L12