title: Polyadenylylated nuclear RNA encoded by Kaposi sarcoma-associated herpesvirus.
abstract: A newly recognized gamma herpesvirus known as Kaposi sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) is present in Kaposi sarcomas and body-cavity-based lymphomas. Here we identify a novel abundant 1.2-kb RNA, polyadenylated nuclear RNA (PAN RNA), encoded by the virus. The majority of cDNAs produced from poly(A)-selected RNA isolated from a human body cavity lymphoma cell line 48 hr after butyrate induction of KSHV lytic replication represented PAN RNA. Within PAN RNA were two 9 and 16 nt stretches with 89% and 94% identity to U1 RNA. A third stretch of 14 nt was 93% complementary to U1. The 5' upstream region of PAN RNA contained both proximal and distal sequence elements characteristic of regulatory regions of U snRNAs, whereas the 3' end was polyadenylylated. PAN RNA was transcribed by RNA polymerase II, lacked a trimethylguanosine cap, and did not associate with polyribosomes. PAN RNA formed a speckled pattern in the nucleus typical of U snRNAs and colocalized with Sm protein. Therefore, PAN represents a new type of RNA, possessing features of both U snRNA and mRNA.
authors.affiliation: Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06520, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sun
authors.forename: S F
authors.initials: SF
authors.lastname: Lin
authors.forename: L
authors.initials: L
authors.lastname: Gradoville
authors.forename: G
authors.initials: G
authors.lastname: Miller
mesh_terms: D001483:Base Sequence; D002051:Burkitt Lymphoma; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D018076:DNA, Complementary; D006151:Guanosine / Q000031:analogs & derivatives; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis*; D012045:Regulatory Sequences, Nucleic Acid; D012514:Sarcoma, Kaposi / Q000821:virology*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014779:Virus Replication / Q000187:drug effects
chemical_list: D002087:Butyrates; D018076:DNA, Complementary; D012342:RNA, Small Nuclear; D012367:RNA, Viral; D020148:Butyric Acid; D006151:Guanosine; C017778:N(2),N(2),7-trimethylguanosine; D012319:RNA Polymerase II