Influence of Carbohydrates on the alpha-Galactosidase Activity of Lactobacillus fermentum
The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; scarce activity was detected with glucose. Exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of L. fermentum growing on galactose. This effect was reversed by the addition of cyclic adenosine-3',5' monophosphate (cAMP), which suggests that this compound could be involved in the regulation of alpha-gal synthesis.
Garro
MS
Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000 Tucuman, Argentina
de Valdez GF
Oliver
G
de Giori GS