title: Primary processing of specimens and isolation and cultivation of mycobacteria.
abstract: Processing of specimens for mycobacteria need to proceed as rapidly as possible to provide 24 hour turnaround for smears and 21-day turnaround for the identification of Mycobacterium tuberculosis. If these goals are to be accomplished, a broth culture or microcolony plate method must be part of the media and in the planting process. Use of conventional solid media alone does not provide adequate turnaround time.
authors.affiliation: Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Hall
mesh_terms: D001431:Bacteriological Techniques; D004396:Coloring Agents; D006801:Humans; D008853:Microscopy; D009161:Mycobacterium / Q000254:growth & development / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D013048:Specimen Handling / Q000592:standards*; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
chemical_list: D004396:Coloring Agents