Molecular imprinting: synthesis of polymer particles with antibody-like binding characteristics for glucose oxidase.
A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polymer particles, the surface bound protein was eluted. In rebinding assays and enzyme activity tests a specific binding capacity for glucose oxidase of up to 0.557 microgram GOD/100 mg dry weight of polymer particles could be determined. These polymer particles have the potential to be used as specific separation or detection material.
Burow
M
M
National Institute of Materials and Chemical Research, Ibaraki, Japan.
Minoura
N
N
D000178:Acrylamides; D000906:Antibodies; D001665:Binding Sites; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D011108:Polymers; D012822:Silicon Dioxide; D013885:Thiosulfates
D000178:Acrylamides; D000906:Antibodies; D011108:Polymers; D013885:Thiosulfates; D012822:Silicon Dioxide; C071115:N,N'-(1,2-dihydroxyethylene)bisacrylamide; C035309:ammonium thiosulfate; D005949:Glucose Oxidase; C021221:N,N'-methylenebisacrylamide