Evaluation of commercial kits for quantitative identification and tests on antibiotic susceptibility of genital mycoplasmas.
In this study, commercial media and kits for identification and tests for antibiotic susceptibility of genital mycoplasmas were evaluated in comparison to standardized methods. A total of 298 specimens was routinely enrolled. Our results showed that all media corresponded for qualitative and semiquantitative identification with a specificity of 100% for both microorganisms and a sensitivity of 94% for U. urealyticum and of 88% for M. hominis. Standard methods were superior to commercial kits for qualitative and quantitative identification (sensitivity 85-97% for U. urealyticum and 79-83% for M. hominis). There were large discrepancies when testing antibiotic susceptibility.
Abele-Horn
M
M
Max von Pettenkofer-Institute, Ludwig-Maximillans-University, Munich, Germany.
Blendinger
C
C
Becher
C
C
Emmerling
P
P
Ruckdeschel
G
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002584:Cervix Uteri / Q000382:microbiology* / Q000473:pathology; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D009174:Mycoplasma / Q000187:drug effects / Q000302:isolation & purification; D019535:Mycoplasma hominis / Q000187:drug effects / Q000302:isolation & purification*; D009305:Nasopharynx / Q000382:microbiology* / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D016990:Ureaplasma urealyticum / Q000187:drug effects / Q000302:isolation & purification*; D014521:Urethra / Q000382:microbiology* / Q000473:pathology
D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic