Treatment with 15-deoxyspergualin induces temporal suppression of hemopoiesis and changes thymocyte populations in mice.
Effects of the immunosuppressive agent, 15-deoxyspergualin (DSG) on hemopoiesis and thymocyte populations were examined in mice. The numbers of bone marrow (BM) cells decreased after the administration of DSG for up to 8 days, and the extent of this decrease was correlated inversely to the time of administration. Conversely, the BM cells of DSG-treated mice had enhanced proliferative responses in vitro and in viv. Deoxymethylspergualin, DSG analogue, directly suppressed the proliferation of BM cells in vitro in a time and dose-dependent manner. Total thymocyte counts decreased following the administration of DSG. The numbers of immature, peanut agglutinin-binding (PNA+) thymocytes decreased notably. Minor changes were observed in the mature thymocytes and spleen cells of DSG-treated mice.
Department of Bacterial and Blood Products, National Institute of Health, Tokyo, Japan.
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Mochida-Nishimura
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Tokunaga
D000818:Animals; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D006131:Growth Inhibitors; D006146:Guanidines; D007166:Immunosuppressive Agents; C037258:gusperimus