The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool.
DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.
Yu
X J
XJ
University of Texas Medical Branch, Galveston 77555-0609, USA.
Crocquet-Valdes
P
P
Cullman
L C
LC
Walker
D H
DH
D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018469:Ehrlichia chaffeensis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect / Q000706:statistics & numerical data; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins