Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding.
The rat sperm surface antigen, 2B1, that has been proposed to play a key role in sperm adhesion to the zona pellucida, has been cloned and its entire cDNA sequenced. Northern blot analysis indicates that 2B1 is encoded by a 2.2-kb RNA transcript that is abundantly expressed in the testis. The deduced protein sequence contains 512 amino-acid residues with a strong candidate signal sequence and C-terminal transmembrane domain. Data base searches reveal a high degree of sequence similarity to guinea pig, rabbit, monkey, and human PH20 sperm surface antigens, and a lower degree of similarity to honey bee and whiteface hornet venom hyaluronidases. Rat 2B1 antigen also possesses hyaluronidase activity, suggesting that it is a bifunctional protein with putative roles in the dispersion of cumulus oophorus cells as well as zona adhesion. However, while it would appear that 2B1 is the rat homologue of the guinea pig PH20 antigen, they differ in a number of important biochemical respects (including their mode of attachment to the sperm membrane and distribution between soluble and membrane-bound fractions), as well as in their localization on the sperm membrane. Expression of regions of the 2B1 protein in recombinant bacterial cells has allowed a preliminary mapping of the 2B1 epitope, and has provided more definitive information on the endoproteolytic processing of 2B1 during epididymal transit.
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
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D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015815:Cell Adhesion Molecules; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D011508:Chondroitin Sulfate Proteoglycans; D006023:Glycoproteins; D006821:Hyaluronoglucosaminidase; C066852:hyaluronidase PH-20