The structure of the gene for the second chain of the human interferon-gamma receptor.
The gene for the second chain of the human interferon-gamma receptor was analyzed from cosmid DNA clones. The gene spans over 33 kilobases of DNA and contains seven exons. The signal peptide is encoded by exons 1 and 2, the extracellular domain by exons 2, 3, 4, 5, and by part of 6. Exon 6 also encodes the whole transmembrane domain and part of the intracellular domain. Exon 7 encodes the remainder of the intracellular domain and contains the 3'-untranslated region. The sequences at the exon/intron boundaries are well conserved with respect to canonical acceptor/donor sites (AG/GT). The 5'-flanking region was sequenced and analyzed for transcription factor binding sites. No TATA or CAAT boxes in the promoter region were identified. Consistent with the lack of a TATA box, analysis of the mRNAs by primer extension showed multiple transcription start sites. Promoter activity of the 5'-flanking region was investigated with a luciferase reporter gene and the cytomegalovirus minimal promoter. Segments of the 5' region with promoter activity were identified.
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635, USA.
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Rhee
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Ebensperger
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Dembic
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Pestka
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017471:Receptors, Interferon / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D000096743:Interferon gamma Receptor
D015703:Antigens, CD; D004274:DNA, Recombinant; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor