Open reading frame 3 of the barotolerant bacterium strain DSS12 is complementary with cydD in Escherichia coli: cydD functions are required for cell stability at high pressure.
Escherichia coli strain JD518, a cydD-deficient mutant, displayed temperature-sensitive and pressure-sensitive growth. The defective cydD gene in this strain was complemented by open reading frame 3 (ORF3), previously identified in DNA from a barotolerant bacterium, strain DSS12, allowing growth of the cydD mutant under high temperature and high pressure conditions. Spectrophotometrical analysis indicated that the cytochrome bd complex which is assembled by the CydD protein was expressed in E. coli strain JD518 carrying the ORF3 gene at the same level as occurred in the wild-type strain. Our results indicate that the cydD gene functions are required for cell stability under the condition of high pressure stress in bacteria.
Kato
C
C
DEEP STAR group, Japan Marine Science and Technology Center, Kanagawa. katoc@jamstec.go.jp
Tamegai
H
H
Ikegami
A
A
Usami
R
R
Horikoshi
K
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D001274:Atmospheric Pressure; D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C089839:CydD protein, E coli; D029968:Escherichia coli Proteins