Attaching and effacing enteropathogenic Escherichia coli O18ab invades epithelial cells and causes persistent diarrhea.
A case of persistent diarrhea following Escherichia coli O18ab gastroenteritis is reported. Electron microscopy of a biopsy of the small intestine showed effacement of the brush border, attachment of bacteria to the epithelial cells with pedestal formation, and bacteria within the enterocytes. The bacterial isolate was an enteropathogenic E. coli isolate which did not contain the adherence factor (EAF) but possessed the attaching-effacing eae gene, was able to invade HeLa cells in a gentamicin invasion assay, and also invaded rabbit intestinal cells. Results suggest that E. coli organisms of the O18ab serotype may cause diarrhea by an as yet unknown pathogenic mechanism, involving attaching to and effacing of enterocytes followed by invasion of the epithelial cells.
Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, Escola Paulista de Medicina, Brazil.
I C
IC
Scaletsky
M Z
MZ
Pedroso
U
U
Fagundes-Neto
D018829:Adhesins, Bacterial; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002352:Carrier Proteins; D003967:Diarrhea / Q000382:microbiology* / Q000473:pathology; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D007583:Jejunum / Q000382:microbiology / Q000473:pathology; D008871:Microvilli / Q000382:microbiology / Q000473:pathology; D011817:Rabbits; D012703:Serotyping; D014774:Virulence
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli