Identification of multiprotein complexes containing DNA replication factors by native immunoblotting of HeLa cell protein preparations with T-antigen-dependent SV40 DNA replication activity.
Increasing evidence has supported the concept that many of the enzymes and factors involved in the replication of mammalian DNA function together as a multiprotein complex. We have previously reported on the partial purification of a multiprotein form of DNA polymerase from human HeLa cells shown to be fully competent to support origin-specific large T-antigen-dependent simian virus 40 (SV40) DNA replication in vitro. In an attempt to more definitively identify the complex or complexes responsible for DNA replication in vitro, partially purified human HeLa cell protein preparations competent to replicate DNA in vitro were subjected to native polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose. The Native Western blots were probed with a panel of antibodies directed against proteins believed to be required for DNA replication in vitro. Apparent complexes of 620 kDa and 500 kDa were identified by monoclonal antibodies directed against DNA polymerase alpha and DNA polymerase delta, respectively. To detect epitopes possibly unexposed within the native multiprotein complexes, blots were also analyzed following denaturation in situ following treatment with detergent and reducing agent. The epitope or access to the epitope recognized by the monoclonal antibody against DNA polymerase alpha was destroyed by exposure of the blots to denaturing conditions. In contrast, an epitope present on a very large complex of approximately 1000 kDa was recognized by a monoclonal antibody against proliferating cell nuclear antigen only following treatment of the native immunoblots with denaturing agents. Identification of these complexes will allow their further purification, characterization, and elucidation of their role in the replication of DNA.
Tom
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TD
Department of Anesthesiology, University of Maryland School of Medicine, Baltimore 21201, USA.
Malkas
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LH
Hickey
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RJ
D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D011948:Receptors, Antigen, T-Cell; D013539:Simian virus 40
D004279:DNA, Viral; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D011506:Proteins; D011948:Receptors, Antigen, T-Cell