Recombinant neutral endopeptidase decreases oedema in the skin of burned guinea-pigs.
Oedema, due to increased vascular leakage postburn, cases significant problems for burn patients. The purpose of this study was to determine if local application of a recombinant neutral endopeptidase (rNEP) would reduce the increased vascular permeability caused by a burn. In a guinea-pig model, a single treatment of rNEP given immediately postburn significantly decreased burn-induced plasma extravasation. This rNEP effect was dependent upon both the dosage of the peptidase and its enzymatic activity. Additional experiments were consistent with the rNEP acting partly by degrading bradykinin, a mediator of increased vascular permeability postburn. These findings suggest that further study of rNEP as a possible treatment for oedema is warranted.
Shriners Burns Institute, Cincinnati, Ohio, USA.
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Neely
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Imwalle
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Holder
D000704:Analysis of Variance; D000818:Animals; D001920:Bradykinin / Q000008:administration & dosage / Q000494:pharmacology*; D002056:Burns / Q000188:drug therapy* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology*; D006168:Guinea Pigs; D007271:Injections, Intradermal; D008297:Male; D015260:Neprilysin / Q000008:administration & dosage / Q000627:therapeutic use*
D015260:Neprilysin; D001920:Bradykinin