S-(-)-nicotine-1'-N-oxide reductase activity of rat liver aldehyde oxidase.
In the present study, a rat liver cytosolic enzyme responsible for reduction of S-(-)-nicotine-1'-N-oxide to S-(-)-nicotine was investigated. We found that aldehyde oxidase (EC 1.2.3.1) can function as a S-(-)-nicotine-1'-N-oxide reductase in the presence of its electron donor such as 2-hydroxypyrimidine. The apparent K(m) and Vmax values of the enzyme for the N-oxide were 0.24 mM and 30.3 nmol/10 min/mg protein, respectively.
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
K
K
Sugihara
S
S
Kitamura
K
K
Tatsumi
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009538:Nicotine / Q000031:analogs & derivatives / Q000378:metabolism*; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D001547:Benzaldehydes; D003497:Cyclic N-Oxides; D004791:Enzyme Inhibitors; D011743:Pyrimidines; C041345:2-hydroxypyrimidine; C024270:nicotine 1-N-oxide; D009538:Nicotine; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; C032175:benzaldehyde