Bacterial killing by use of once daily gentamicin dosage in guinea pigs with Escherichia coli infection.
OBJECTIVE
To determine whether the antibacterial activity of 6 mg of gentamicin/kg of body weight given SC once daily, is equivalent to the standard gentamicin dose of 2 mg/kg given SC every 8 hours.


METHODS
Guinea pigs with infected thigh wound: 5 in an untreated control group and 12 in 6 and 2 mg/kg gentamicin treatment groups.


METHODS
Guinea pigs were inoculated with 10(9) Escherichia coli in the thigh muscle. Gentamicin treatment (2 mg/kg, SC, q 8 h or 6 mg/kg, SC, q 24 h) was begun 4 hours after E coli inoculation and continued for 72 hours. Four hours after the last gentamicin treatment, all guinea pigs were euthanatized and the cranial thigh muscle containing the entire inoculum was removed. Colony-forming units were counted to determine the E coli concentration in each thigh.


RESULTS
Mean +/- SD log10 colony-forming units was 9.293 +/- 0.074 in the control group, 8.161 +/- 0.478 in the 2 mg/kg treatment group, and 7.796 +/- 0.182 in the 6 mg/kg treatment group. One-way ANOVA revealed a significant (P < 0.05) difference between the control group and both treatment groups, and between both treatment groups.


CONCLUSIONS
Bacterial killing did not differ between gentamicin given at a dosage of 6 mg/kg once daily, compared with 2 mg/kg every 8 hours in guinea pigs infected with E coli.


CONCLUSIONS
Gentamicin dosage regimens with high peak concentration and long dosing interval are as efficacious as divided dosage regimens. These data support the concept that once daily administration of gentamicin for treatment of E coli infection should be investigated clinically.
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
B G
BG
Campbell
S
S
Bartholow
E
E
Rosin
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000662:veterinary*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006168:Guinea Pigs / Q000382:microbiology*; D008297:Male; D012376:Rodent Diseases / Q000188:drug therapy* / Q000382:microbiology
D000900:Anti-Bacterial Agents; D005839:Gentamicins