HT29-MTX/Caco-2 cocultures as an in vitro model for the intestinal epithelium: in vitro-in vivo correlation with permeability data from rats and humans.
The diverse secretory and absorptive functions of the intestinal epithelium are conducted by a mixed population of absorptive cells and mucus-producing goblet cells as the major cell types. In order to approach the main characteristics in an in vitro model, a coculture system of absorptive Caco-2 cells and mucus-secreting HT29-MTX cells was developed and the permeability of a range of different drugs was tested. Variable goblet cell frequency can be achieved, preserving a significant barrier to drug transport and maintaining the differentiated features of both cell types. Absorption rates for actively transported drugs are rather underestimated in the cell culture model when compared to in vivo data. However, a good correlation with fraction absorbed in humans was attained separating the range of passively transported drugs into two groups of well-absorbable compounds with Peff > or = 10 x 10(-6) cm/s and drugs that are absorbed 40-70% with Peff = 0.1-1 x 10(-5) cm/s. A permeability of Peff < 0.1 x 10(-5) cm/s is suggested for low absorbable drugs.
Walter
E
E
College of Pharmacy, University of Michigan, Ann Arbor 48109-1065, USA.
Janich
S
S
Roessler
B J
BJ
Hilfinger
J M
JM
Amidon
G L
GL
D000818:Animals; D001693:Biological Transport, Active; D018938:Caco-2 Cells / Q000378:metabolism* / Q000648:ultrastructure; D002506:Cephalexin / Q000378:metabolism; D018920:Coculture Techniques; D019073:HT29 Cells / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008750:Methyldopa / Q000378:metabolism; D008954:Models, Biological; D009093:Mucus; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011433:Propranolol / Q000378:metabolism; D051381:Rats
D004364:Pharmaceutical Preparations; D008750:Methyldopa; D011433:Propranolol; D002506:Cephalexin