Polarized targeting of a shaker-like (A-type) K(+)-channel in the polarized epithelial cell line MDCK.
Functional Kv 1-4 channels were stably expressed in filter-grown MDCK cells which form a polarized epithelium with two distinct plasma membrane domains: a basolateral and an apical cell surface. The Shaker-related Kv 1-4 channels mediated in MDCK cells fast transient (A-type) voltage-activated outward currents having similar properties to the ones reported for Kv 1-4 in the Xenopus oocytes expression system. Immunoblot analysis with specific anti-Kv 1-4 antibodies showed that two Kv 1-4 protein forms are expressed in MDCK cells which most likely represent the glycosylated and non-glycosylated Kv 1-4 protein, respectively. Using immunocytochemistry and confocal microscopy we showed that the Kv 1-4 channels are specifically localized in the basolateral membranes of MDCK cells. Thus, the MDCK cells may provide an important model system to analyse the polarized transport of ion channels such as Kv 1-4, which are distinctly expressed in the mammalian central nervous system.
Department de Biologie Cellulaire et Moléculaire, CEA Saclay, Gif sur Yvette, France.
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Le Maout
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Sewing
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Coudrier
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Elalouf
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Pongs
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Merot
D001692:Biological Transport; D002460:Cell Line / Q000378:metabolism; D016764:Cell Polarity; D004847:Epithelial Cells; D051665:Kv1.4 Potassium Channel; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D014162:Transfection
D051665:Kv1.4 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated