Evidence for a single pool of myo-inositol in hormone-responsive WRK-1 cells.
Previous reports have suggested the existence of at least two pools of cellular myo-inositol (Ins); it has been further hypothesized that only one of these pools is utilized during hormone-activated, cyclic phosphatidylinositol (PtdIns) resynthesis. In an effort to investigate this possibility, we have undertaken kinetic studies of Ins metabolism in WRK-1 cells. Our results indicate that a single pool of Ins is involved in both basal and activated PtdIns synthesis. Ins generated by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) mixes with the existing pool of free Ins and is not used exclusively for resynthesis of PtdIns.
Monaco
M E
ME
Department of Physiology and Biophysics, New York University Medical Center, 10010, USA.
Moldover
N H
NH
D000818:Animals; D007294:Inositol / Q000032:analysis*; D007295:Inositol Phosphates / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000494:pharmacology
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D014667:Vasopressins; D007294:Inositol; D008094:Lithium