Partial thermodynamic parameters for prediction stability and washing behavior of DNA duplexes immobilized on gel matrix.
Earlier we showed that reported in literature nearest-neighbor thermodynamic parameters describe poorly the thermal-induced behavior of DNA duplexes immobilized in gel. Here we present a complete set of partial thermodynamic parameters for all 10 nearest-neighbor interactions specially developed for duplexes immobilized in gel. This thermodynamic library allows to predict dissociation enthalpy and free energy of DNA duplex immobilized in gel matrix from its base sequence. The predicted values are in good agreement with the experimental ones. Dissociation enthalpy and free energy are needed for such application as (i) predicting relative stability of duplexes formed by DNA with oligonucleotides immobilized in cells of gel matrix; (ii) selecting optimal conditions for hybridization experiment; (iii) predicting washing curves and washing temperatures at irreversible temperature-stepped wash of DNA out of oligonucleotide gel matrix; (iv) selecting optimal conditions for washing gel matrix.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russian Federation.
A
A
Kunitsyn
S
S
Kochetkova
E
E
Timofeev
V
V
Florentiev
D000180:Acrylic Resins / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D008958:Models, Molecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013816:Thermodynamics
D000180:Acrylic Resins; D005782:Gels; D009838:Oligodeoxyribonucleotides; C016680:polyacrylamide gels