Identification, isolation and sequence of the Aspergillus nidulans xlnC gene encoding the 34-kDa xylanase.
The xlnC gene encoding the 34-kDa xylanase (X34) of Aspergillus nidulans (An) has been cloned and sequenced, as has its corresponding cDNA. xlnC contains nine introns and shows considerable similarity to the xynA and xylP xylanase-encoding genes of A. kawachii (Ak) and Penicillium chrysogenum (Pc), respectively. Analysis of xylanase production in An multicopy transformants showed elevated levels of X34 and increased total xylanase activity, but no elevated production of other xylanases. Northern analysis demonstrated transcriptional induction by xylan and repression by glucose.
Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain.
A P
AP
MacCabe
M T
MT
Fernández-Espinar
L H
LH
de Graaff
J
J
Visser
D
D
Ramón
D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D043364:Endo-1,4-beta Xylanases; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017422:Sequence Analysis, DNA; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases