The use of transposable P-elements of Drosophila melanogaster for introductory genetics laboratory courses.
Laboratories in introductory genetics courses often use Drosophila melanogaster as a tool for the students to understand how genes are transmitted and mapped. We demonstrate three advantages of using P-element containing inserts with the wild-type alleles of the genes to be mapped. First, inserts can be positioned at many places in the genome with relative ease; second, stocks can be chosen which have excellent viability; and third, inserts can be selected for precision in mapping with regard to marker genes. The results of a preliminary test with a beginning genetics laboratory are presented. The inserts exhibit excellent Mendelian ratios in F2 and backcross progeny and were reproducibly mapped by different student groups.
Section of Genetics and Development, Cornell University, Ithaca, New York, USA.
P
P
Morcillo
R
R
Tuttle
R J
RJ
MacIntyre
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005823:Genetics / Q000193:education; D008297:Male; D016254:Mutagenesis, Insertional; D010865:Pilot Projects
D004251:DNA Transposable Elements