Expression of decorin mRNA in the skin of patients with ossification of the posterior longitudinal ligament.
Ossification of the posterior longitudinal ligament (OPLL) is an intractable disease developing into severe myelopathy and often accompanied by ossification of several other spinal ligaments and articular ligaments. Therefore, it is highly probable that predisposition to systemic ossification underlies the onset of this disease. Terayama et al(1981) reported that the nuchal skin of OPLL patients tended to be tougher than that of healthy individuals. Decorin is a component of the extracellular matrix which antagonistically regulates the action of Transforming growth factor-beta (TGF-beta). Imamura et al(1995) demonstrated immunohistochemically that decorin increased in the epidermis of the nuchal skin of OPLL patients. This suggests an abnormal expression of the extracellular matrix in the skin of OPLL patients. In the present study, in situ hybridization with non-radioactive synthetic oligodeoxynucleotide probes revealed an enhanced expression of decorin mRNA in the epidermal keratinocytes of OPLL patients. The increased expression of decorin mRNA in the epidermis of OPLL patients may be interpreted as reflecting abnormalities of the matrix associated with ossification of the ligaments. Studies on the role of decorin in ossification will contribute to clarification of the pathophysiology and pathogenesis of OPLL.
Department of Orthopaedic Surgery, Kagoshima University, Japan.
Y
Y
Ishidou
M
M
Tokunaga
F
F
Murata
H
H
Yoshida
T
T
Sakou
D000328:Adult; D000368:Aged; D016375:Antisense Elements (Genetics); D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009333:Neck / Q000473:pathology; D017887:Ossification of Posterior Longitudinal Ligament / Q000378:metabolism / Q000473:pathology*; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012867:Skin / Q000737:chemistry* / Q000473:pathology
D016375:Antisense Elements (Genetics); C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger