Early supernatants of mitogen-stimulated mononuclear cells promote long-term IL-2-dependent growth of human T lymphocytes.
Human wheat germ agglutinin (WGA)- or PHA-activated T lymphocytes from either peripheral blood or normal spleens can be propagated in vitro for several months in the presence of IL-2 but not IL-4 when periodically restimulated with supernatants collected from 24 h cultures of mitogen-stimulated human blood or spleen mononuclear cells. Following several rounds of stimulation with these supernatants some of the spleen-derived T lymphoblast lines acquire the ability of continuous growth (over 100 population doublings) in the presence of IL-2 alone. We postulate that the ability of 24 h supernatants from mitogen stimulated mononuclear cells to maintain T lymphoblast IL-2-dependent growth depends upon yet undefined cytokine(s) released early after activation.
Department of Immunology, Maria Sklodowska-Curie Memorial Cancer Center, Warsaw, Poland.
J K
JK
Siwicki
J A
JA
Steffen
D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D004357:Drug Synergism; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D014909:Wheat Germ Agglutinins; D015847:Interleukin-4