Development of low potassium solution (EP4 solution) for long-term preservation of a lung transplant: evaluation in primate and murine lung transplant model.
The results of recent studies on long-term preservation using low potassium flush solution, which was originally produced in our department, are presented and discussed. In the primate model, a lung preserved for 24 h with EP4 solution retained sufficient function in single lung allotransplantation. In the murine experiments, we have evaluated the ion transport properties across alveolar epithelial cells in the transplanted lung using the tools of alveolar fluid clearance tests. The alveolar fluid clearance rate, which can be inhibited by 10(-3) M ouabain, is also well preserved for the first 24 h, indicating that the alveolar epithelial Na-K-ATPase is still functioning and that the 72 h preservation decreases those function of Na-K-ATPase in a time-dependent manner. In conclusion, because 24 h hypothermic preservation with EP4 solution did not show any obvious disadvantage on primate lung allografts and the cellular and molecular viability of the preserved lung flushed with EP4 solution could be retained for 24 h, this kind of solution with extracellular electrolyte composition can be applicable in the clinical settings.
Fujimura
S
S
Department of Thoracic Surgery, Tohoku University, Sendai, Japan.
Kondo
T
T
Handa
M
M
Ohura
H
H
Saito
R
R
Sugita
M
M
Suzuki
S
S
D000818:Animals; D001784:Blood Gas Analysis; D002470:Cell Survival; D003080:Cold Temperature; D004195:Disease Models, Animal; D004285:Dogs; D004573:Electrolytes / Q000378:metabolism; D006084:Graft Rejection / Q000188:drug therapy / Q000517:prevention & control; D006085:Graft Survival / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016040:Lung Transplantation / Q000009:adverse effects / Q000592:standards*; D008251:Macaca; D051379:Mice; D011188:Potassium / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014021:Tissue Preservation / Q000592:standards*; D014184:Transplantation, Homologous / Q000592:standards
D004573:Electrolytes; D007166:Immunosuppressive Agents; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium