Molecular cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells.
Cold acclimation of rainbow trout cells is considered to be mediated by alterations in the mRNAs and proteins present in cold-treated cells. A subtracted cDNA library from cold-treated rainbow trout RTG-2 cells was constructed and screened to isolate cDNA induced in the cold-treated cells in order to elucidate which genes are induced by cold acclimation. A set of cDNA clones encoding three members of ferritin H isoforms was isolated as cold-inducible genes. Northern blot analysis and nuclear run-on transcription assay showed that the transcription and accumulation of the ferritin H isoforms mRNA were enhanced by cold acclimation. Furthermore, the ferritin level in the trout cells increased on cold acclimation in response to a temperature shift from 22 degrees C to 4 degrees C. When the trout cells were subjected to 4 degrees C under the condition of a decreased ferritin H level obtained by the addition of an antisense oligonucleotide, cell growth was apparently inhibited. These findings indicate an association between the induction of ferritin H and cellular mechanisms during cold acclimation of trout cells.
Yamashita
M
M
National Research Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama 236, Japan.
Ojima
N
N
Sakamoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D005293:Ferritins / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D005293:Ferritins