Poly(A) metabolism in Xenopus laevis embryos: substrate-specific and default poly(A) nuclease activities are mediated by two distinct complexes.
The metabolism of the poly(A) tail is a process important for the translational regulation of maternal mRNAs in Xenopus laevis oocytes and early embryos. Two poly(A) nuclease (PAN) activities have been described in Xenopus embryo or activated egg extracts (Legagneux et al (1995) RNA 1, 1001-1008). These activities (default PAN and EgPAN) are distinguishable by their deadenylation kinetics and their substrate specificities. In this report, we show that these activities display different sensitivities to biochemical treatments. Urea and, to a lesser extent, spermidine, inhibit EgPAN at concentrations which have no effect on default PAN. Heparin activates default PAN but inhibits EgPAN. When extracts are fractionated by ultracentrifugation, the default activity is recovered in one unique fraction, whereas two fractions must be combined to reconstitute the EgPAN activity. Moreover, these two deadenylation activities are separable by size exclusion chromatography under native conditions. We conclude that these two deadenylation activities are mediated by two protein complexes.
Biologie et Génétique du Développement, CNRS UPR 41, Université de Rennes 1, France.
L
L
Paillard
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V
Legagneux
H B
HB
Osborne
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000494:pharmacology; D009865:Oocytes / Q000201:enzymology*; D011700:Putrescine / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013379:Substrate Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014461:Ultracentrifugation; D014508:Urea / Q000494:pharmacology; D014982:Xenopus laevis / Q000196:embryology
D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D014508:Urea; D006493:Heparin; D012260:Ribonucleases; C022406:poly A hydrolase; D013095:Spermidine; D011700:Putrescine