Mouse parenchymal liver cells in culture secrete a growth inhibitor for myeloma cells.
METHODS
Growth inhibitory activity in the conditioned medium of mouse parenchymal liver cells was examined in three strains of myeloma cells.


RESULTS
Two strains of myeloma cells were highly sensitive to a low concentration of mouse parenchymal liver cell derived growth inhibitor, whereas one strain was resistant to the same concentration. Interferon-alphabeta and transforming growth factor-beta activity were detected in mouse parenchymal liver cells, while interferon-gamma and tumor necrosis factor-alpha were not. The growth suppression exerted by mouse parenchymal liver cell derived growth inhibitor in the three myeloma strains was distinct from that exerted by transforming growth factor-beta, tumor necrosis factor-alpha, interferon-alphabeta and interferon-gamma. The mouse parenchymal liver cell derived growth inhibitor was eluted with a peak activity in the 18 kDa range and focused into pI values of 3.8-4.0, and it was lost when mouse parenchymal liver cells were treated with heat or trypsin.


CONCLUSIONS
These results indicate that mouse parenchymal liver cell derived growth inhibitor differs from the well-characterized growth inhibitors, transforming growth factor-beta, tumor necrosis factor-alpha, interferon-alphabeta and interferon-gamma.
Nippon Medical School, Department of Microbiology and Immunology, Tokyo, Japan.
M
M
Takahashi
K
K
Yokomuro
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D006131:Growth Inhibitors / Q000378:metabolism*; D007372:Interferons / Q000494:pharmacology; D007525:Isoelectric Focusing; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000188:drug therapy*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D017077:Culture Media, Conditioned; D006131:Growth Inhibitors; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D007372:Interferons