Regional and developmental variations in metabolite concentration in the rat brain and eye: a study using 1H NMR spectroscopy and high performance liquid chromatography.
Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to adult, while the related compound N-acetylaspartylglutamate was highest in adult optic nerve. Creatine and choline-containing compounds were present in all regions throughout development, with higher levels of creatine found in grey matter compared to other regions. Choline-containing compounds were present at the highest concentrations in the eye at all ages examined, and tended to decrease in concentration to minimum values in adulthood in all regions. The presence of hypotaurine in corpus callosum and optic nerve was consistent with the metabolic profiles of O-2A progenitor cells and oligodendrocytes, which are cells composing these tissues. The neurotransmitters glutamate and GABA reached their highest concentrations in the olfactory bulb (higher than in adult cortex).
The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, United Kingdom.
C L
CL
Florian
S R
SR
Williams
K K
KK
Bhakoo
M D
MD
Noble
D000375:Aging / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003337:Corpus Callosum / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D005123:Eye / Q000254:growth & development / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D009830:Olfactory Bulb / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism; D009900:Optic Nerve / Q000254:growth & development / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D013234:Stem Cells / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D004151:Dipeptides; C027172:isospaglumic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006859:Hydrogen; C000179:N-acetylaspartate