Production of interleukin-10 in the cerebrospinal fluid in aseptic meningitis of children.
IL-10 is a cytokine that has antiinflammatory properties. We investigated IL-10 using ELISA and a reverse-transcribed polymerase chain reaction in the cerebrospinal fluid (CSF) of children with or without aseptic meningitis. When the patients with aseptic meningitis had meningeal symptoms, IL-10 in the CSF was detectable in 14 of 22 patients (88 +/- 146 ng/L, n = 31). The IL-10 levels decreased as meningeal symptoms disappeared. In 20 of 21 control children without meningitis, CSF samples had no detectable levels of IL-10 (< 10 ng/L). Serum IL-10 levels were lower than the corresponding levels in the CSF from the same individuals with aseptic meningitis. Significant correlations were found between IL-10 levels and mononuclear cell counts in the CSF of the affected patients (r = 0.644, p < 0.001). The IL-10 mRNA was detected by reverse-transcribed polymerase chain reaction-assisted amplification in the CSF cells in four of seven patients with the disease. The culture of CSF mononuclear cells produced high levels of IL-10 (152-485 ng/L) in all of five patients. Cytokine kinetics in the CSF showed that mean IL-10 levels reached the peak on the 2nd to 3rd d of the illness, although all mean levels of IL-6, IL-8, and granulocyte colony-stimulating factor were the highest on the 1st d of the illness. In summary, IL-10 is produced in the CSF in aseptic meningitis, and may increase relatively late compared with the proinflammatory cytokines. IL-10 may play an immunoregulatory role in the meningeal inflammatory network.
Department of Pediatrics, Mizonokuchi Hospital, Teikyo University School of Medicine, Kawasaki, Japan.
A
A
Ishiguro
Y
Y
Suzuki
Y
Y
Inaba
A
A
Komiyama
H P
HP
Koeffler
T
T
Shimbo
D000293:Adolescent; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000166:cytology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000134:cerebrospinal fluid; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000096:biosynthesis / Q000134:cerebrospinal fluid*; D015850:Interleukin-6 / Q000134:cerebrospinal fluid; D016209:Interleukin-8 / Q000134:cerebrospinal fluid; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D013997:Time Factors
D015850:Interleukin-6; D016209:Interleukin-8; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor