Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology.
This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.
Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
B L
BL
Robbins
B H
BH
Waibel
A
A
Fridland
D000998:Antiviral Agents / Q000032:analysis* / Q000493:pharmacokinetics; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000378:metabolism; D010710:Phosphates / Q000032:analysis; D011863:Radioimmunoassay; D015215:Zidovudine / Q000032:analysis* / Q000493:pharmacokinetics
D000998:Antiviral Agents; D010710:Phosphates; D015215:Zidovudine