New variants of enzyme immunoassay of antibodies to DNA.
A method of DNA immobilization on cellulose nitrate films has been developed. Modified films of uniform and stable surface have been used to devise two variants of solid-phase enzyme immunoassays of antibodies. The co-immobilization of enzyme label (cholinesterase) and the DNA molecules makes it possible to carry out the procedure of solid-phase enzyme immunoassay without any separation of components. Thus, it takes only 15 min to diagnose an autoimmune disease (Aleutian disease of minks) with the immunoenzyme amperometric sensor, with a lower detection limit for antibodies of 0.5 x 10(-10) M. For scaled diagnosing, solid-phase enzyme immunoassay on DNA-modified films with prior separation of components and spectrophotometric registration of peroxidase activity has been developed. The time for determination was 30 min, with a lower detection limit of 7.4 x 10(-12) M.
Chemical Faculty, Kazan State University, Russia.
S S
SS
Babkina
E P
EP
Medyantseva
H C
HC
Budnikov
M P
MP
Tyshlek
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D004247:DNA / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008907:Mink; D013053:Spectrophotometry
D000974:Antibodies, Antinuclear; D004247:DNA