Temporal dissociation of neurotensin/neuromedin N mRNA expression in topographically separate subsets of rat striatal neurons following administration of haloperidol.
The expression of the neurotensin/neuromedin N gene in the rat caudate-putamen was studied at 7 and 24 h following the administration of haloperidol using a riboprobe and non-isotopic in situ hybridization histochemistry. As has been reported by others, expression of neurotensin/neuromedin N mRNA in the dorsolateral quadrant of the caudate-putamen was undetectable in controls, robust at 7 h and minimally detectable at 24 h following haloperidol administration. In the dorsomedial and ventrolateral quadrants of the caudate-putamen, barely detectable basal expression of neurotensin/neuromedin N mRNA observed in controls was conspicuously enhanced at 24 h following administration of haloperidol both in terms of numbers of hybridized neurons, which were about 200% of control values, and the amount of chromogen accumulated over individual neurons. The data are consistent with at least two subpopulations of caudate-putamen neurons in which neurotensin/neuromedin N mRNA expression is differentially regulated in response to haloperidol administration.
Department of Anatomy and Neurobiology, St. Louis University School of Medicine, MO 63104, USA. zahmds@wpogate.slu.edu
D S
DS
Zahm
E S
ES
Williams
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D
Poulad
J E
JE
Krause
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001931:Brain Mapping; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006220:Haloperidol / Q000494:pharmacology*; D006651:Histocytochemistry / Q000379:methods; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009496:Neurotensin / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D014150:Antipsychotic Agents; D010446:Peptide Fragments; D012333:RNA, Messenger; C042090:neuromedin N; D009496:Neurotensin; D006220:Haloperidol