Determination of N-nitroso-L-arginine in rat brain extracts by capillary electrophoresis using photodiode-array detection.
N-Nitroso-L-arginine was described as one of the products of L-arginine metabolism in biological media. A simple and rapid method to determine its concentration in rat brain was developed. Capillary electrophoresis with a photodiode-array detector was used at 254 nm, permitting the quantification of N-nitroso-L-arginine. The detection limit in biological solution was 1 microgram/ml.
Faculté de Médecine Xavier-Bichat, Laboratoire de Biophysique, Paris, France.
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Meulemans
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry / Q000502:physiology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009603:Nitroso Compounds / Q000032:analysis*; D051381:Rats; D012680:Sensitivity and Specificity
D009603:Nitroso Compounds; C084185:N-nitrosoarginine; D001120:Arginine