Real time observation of the binding of herpes simplex virus type 1 (HSV-1) to immobilized heparan sulfate and the neutralization of HSV-1 by sulfonated human immunoglobulin.
A real time biomolecular interaction assay system involving an optical sensor was applied to quantitative analysis of the binding of herpes simplex virus type 1 (HSV-1) to immobilized heparan sulfate, a cellular receptor component of HSV-1, and the neutralization antibody titer against this virus with a commercially available sulfonated human immunoglobulin preparation. The virus titer in a viral solution and the neutralizing antibody titer in the human immunoglobulin preparation could be successfully estimated in a short time with this system without any difficult cell culture.
Inoue
K
K
Department of Microbiology, Meiji College of Pharmacy, Tokyo.
Arai
T
T
Aoyagi
M
M
D000818:Animals; D019138:Antibodies, Blocking / Q000378:metabolism; D000914:Antibodies, Viral / Q000378:metabolism*; D015374:Biosensing Techniques; D002522:Chlorocebus aethiops; D006497:Heparan Sulfate / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009500:Neutralization Tests; D011991:Receptors, Virus / Q000378:metabolism; D014709:Vero Cells
D019138:Antibodies, Blocking; D000914:Antibodies, Viral; D006497:Heparan Sulfate; D011991:Receptors, Virus