Increase of murine splenic natural antibody-secreting cells after cyclophosphamide treatment.
Administration of a low sub-immunosuppressive dose of cyclophosphamide (CY) to naive mice induced a marked increase in the number of splenic cells forming natural antibodies against unrelated antigens such as foot-and-mouth disease virus, keyhole limpet hemocyanin, horseradish peroxidase, or bovine serum albumin, as determined by an enzyme-linked immunosorbent assay spot technique. These results suggest that in mice there exists a repertoire of B cells forming natural antibodies which is restrained by an unknown mechanism and that CY is able to interfere with that restriction.
Portiansky
E L
EL
Cátedra de Patología General Veterinaria, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
González
P H
PH
Laguens
R P
RP
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology*; D002452:Cell Count / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology*
D003520:Cyclophosphamide