Flow cytometric identification of murine neutrophils and monocytes.
Flow cytometry and cell sorting have been employed in order to identify and purify murine neutrophils and monocytes. Using a combination of antibodies, we were able to distinguish between these two subsets of myeloid cells. The method described in this paper is simple to perform and can be applied to characterize myeloid cells from blood, spleen, bone marrow and after an induced inflammation.
Lagasse
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Department of Pathology and Developmental Biology, Stanford University School of Medicine, CA 94305, USA.
Weissman
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IL
D000818:Animals; D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology*; D013154:Spleen / Q000166:cytology; D013864:Thioglycolates
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D016177:Macrophage-1 Antigen; D013864:Thioglycolates; D017493:Leukocyte Common Antigens