Role of alpha 2 beta 1- and alpha 3 beta 1-integrin in the peritoneal implantation of scirrhous gastric carcinoma.
We established a highly peritoneal-seeding cell line, OCUM-2MD3, from a poorly peritoneal-seeding cell line, OCUM-2M, of human scirrhous gastric carcinoma. The intraperitoneal inoculation of OCUM-2MD3 cells produced peritoneal dissemination in nude mice, whereas that of OCUM-2M cells did not. We then investigated the correlation between seeding potential and adhesion molecule beta 1-integrins or alpha 6 beta 4-integrin. alpha 2 beta 1- and alpha 3 beta 1-integrin expression on OCUM-2MD3 cells (91.6% and 93.6%) was increased compared with that of OCUM-2M cells (47.8% and 34.3%) by flow cytometric analysis, and the expression level of the other integrins was not different between the two cell lines. The binding ability of OCUM-2MD3 cells to matrigel, fibronectin, laminin and type I collagen was significantly increased, approximately seven times, three times, eight times, and three times greater than that of OCUM-2M cells respectively. The invasiveness of OCUM-2MD3 cells was also significantly increased 8-fold over OCUM-2M cells. The binding and invasive ability of OCUM-2MD3 cells was significantly decreased following the addition of anti-alpha 2 beta 1- and alpha 3 beta 1-integrin antibody, but not by anti-alpha 6 beta 1- and alpha 6 beta 4-integrin antibody. These results suggest that adhesiveness and invasiveness in peritoneal implantation of scirrhous gastric carcinoma might be closely associated with alpha 2 beta 1- and alpha 3 beta 1-integrin.
First Department of Surgery, Osaka City University Medical School, Japan.
S
S
Nishimura
Y S
YS
Chung
M
M
Yashiro
T
T
Inoue
M
M
Sowa
D002293:Adenocarcinoma, Scirrhous / Q000378:metabolism / Q000556:secondary*; D000818:Animals; D002448:Cell Adhesion; D003094:Collagen; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000378:metabolism / Q000502:physiology*; D007797:Laminin; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism / Q000502:physiology*; D010534:Peritoneal Neoplasms / Q000378:metabolism / Q000556:secondary*; D011509:Proteoglycans; D038981:Receptors, Collagen; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D004338:Drug Combinations; D039201:Integrin alpha3beta1; D016023:Integrins; D007797:Laminin; D009363:Neoplasm Proteins; D011509:Proteoglycans; D038981:Receptors, Collagen; C059485:matrigel; D003094:Collagen