Genetic diversity of flagellins of Pseudomonas aeruginosa.
Physical genome analysis of the virulence-associated fliC locus in 20 Pseudomonas aeruginosa strains by mapping and sequencing revealed groups of heterologous a-type (1164 bp; 1185 bp) and highly conserved b-type (1467 bp) flagellin genes. Whereas only two synonymous nucleotide substitutions were detected in eight b-type fliC sequences, the 12 a-type sequences exhibited 57 nucleotide substitutions, of which 39 occurred within a variable central region. Although a-type and b-type flagellins differ by 35% in their primary structure, they share strong homology in their predicted features, implying that the polymorphic proteins fold into similar structures during polymerization of the flagella.
Spangenberg
C
C
Klinische Forschergruppe, Medizinische Hochschule Hannover, Max-Planck-Institut für experimentelle Endokrinologie, Germany.
Heuer
T
T
Bürger
C
C
Tümmler
B
B
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D017124:Conserved Sequence; D036781:Fimbriae Proteins; D005408:Flagellin / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; D005408:Flagellin; D036781:Fimbriae Proteins