Enteroviral polymerase chain reaction in the investigation of aseptic meningitis.
We used a nested polymerase chain reaction (nPCR) to seek evidence for enteroviruses in clinical samples from patients with symptoms of aseptic meningitis. When compared with conventional virus isolation methods on a total of 366 samples collected during 1994-1995, an increase in positivity from 6% to 27% was shown. The results indicate that nPCR would be a valuable aid to the laboratory diagnosis of enteroviral infections as it can detect those enteroviruses that cannot be identified by current isolation methods.
Riding
M H
MH
Scottish Centre for Infection and Environmental Health, Ruchill Hospital, Glasgow, Scotland.
Stewart
J
J
Clements
G B
GB
Galbraith
D N
DN
D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008253:Macaca mulatta; D008297:Male; D008587:Meningitis, Viral / Q000821:virology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D004279:DNA, Viral