Glutathione plays a role in the chick intestinal calcium absorption.
DL-buthionine-S,R-sulfoximine (BSO) administration to vitamin D-deficient chicks treated with cholecalciferol produces a rapid decrease in the Ca2+ transfer from lumen-to-plasma and in the intestinal glutathione content. This response was reversed by addition of glutathione monoester to the intestinal sac. Variables related to the Ca2+ homeostasis such as plasma Ca and P, and intestinal calbindin D28k were not modified by BSO given to vitamin D-deficient chicks treated with cholecalciferol. Intestinal alkaline phosphatase activity, on the contrary, was highly reduced by BSO in vitamin D-deficient chicks treated with vitamin D3. This effect showed time and dose-dependency. Although the mechanism/s of action of BSO on the intestinal Ca absorption is unknown, it is quite possible that thiol groups of protein involved in the Ca2+ transport are affected by the GSH depletion and/or by block of the antioxidant ability of vitamin D3. Thus, reactive oxygen compounds would be increased and, therefore, the Ca2+ movement from lumen to plasma decreases.
Cătedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
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N
Tolosa de Talamoni
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Marchionatti
V
V
Baudino
A
A
Alisio
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D064026:Calbindins; D002118:Calcium / Q000493:pharmacokinetics*; D002132:Calcium Radioisotopes; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D005978:Glutathione / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology; D008566:Membranes / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D014808:Vitamin D Deficiency / Q000378:metabolism
D000963:Antimetabolites; D064026:Calbindins; D002132:Calcium Radioisotopes; D064030:S100 Calcium Binding Protein G; D019328:Buthionine Sulfoximine; D000469:Alkaline Phosphatase; D005978:Glutathione; D002118:Calcium