Hemoglobin damages the cultured endothelial cell monolayer from bovine cerebral arteries.
We demonstrated that hemoglobin reaches the endothelial layers by an immunoelectron microscopic analysis in a rabbit subarachnoid hemorrhage model. Next, the effects of hemoglobin on cerebral artery endothelial cells were investigated both morphologically and histochemically using a cultured monolayer from a bovine endothelial cell system. The cultured monolayer was incubated with six different concentrations of hemoglobin ranging from 0 to 10(-5) M for 24 hrs on two-chambered glass slides. The denuded area of the glass slide increased dose dependently while the intensity of the actin fiber decreased. Superoxide dismutase did not modify this change. In a preliminary study, the endothelial cell membrane showed a hemoglobin binding molecule (94.5k dalton). This study thus suggests that the effects of hemoglobin on the endothelial monolayer were partly caused by the direct binding of hemoglobin to the membrane.
Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
T
T
Machi
N F
NF
Kassell
Y
Y
Tanaka
S
S
Hudson
G A
GA
Lehman
T P
TP
Harrison
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006454:Hemoglobins / Q000502:physiology*; D011817:Rabbits
D006454:Hemoglobins