Prostaglandins and the antiarrhythmic effect of preconditioning in the isolated rat heart.
Our study evaluated the relationship between the endogenous production of prostacyclin and the antiarrhythmic effect of ischemic preconditioning against ischemic and reperfusion-induced tachyarrhythmia. Langendorff perfused rat hearts underwent 30 min regional ischemia with reperfusion. Preconditioning was induced by a single episode of 5 min ischemia and 15 min reperfusion. Prostaglandin 6-keto F1 alpha (a stable metabolite of prostacyclin) was determined in the coronary effluent. In the control group the incidence of tachyarrhythmia was 31% during ischemia and 67% during reperfusion. Preconditioning did not affect ischemic arrhythmias but attenuated arrhythmias a reperfusion (8%, p < 0.01) and was associated with increased release of prostacyclin prior to reperfusion. Aspirin abolished the antiarrhythmic effect of preconditioning against reperfusion tachyarrhythmias. However, no relationship was found between suppression of prostacyclin production and the occurrence of arrhythmia in individual hearts. Thus, our findings suggest that metabolites of arachidonic acid via the cyclooxygenase pathway are involved in the protective effect of ischemic preconditioning against reperfusion-induced tachyarrhythmias.
Cardiac Metabolism Laboratory, Sheba Medical Center, Tel Hashomer, Israel.
M
M
Arad
T
T
Oxman
R
R
Leor
B
B
Rabinowitz
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D001241:Aspirin / Q000494:pharmacology; D003326:Coronary Circulation; D006321:Heart / Q000187:drug effects; D006339:Heart Rate; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha; D001241:Aspirin