Identification of bovine CD14.
Six monoclonal antibodies (mAbs) that clustered together in a statistical analysis of data submitted to the Third Workshop on Ruminant Leucocyte Antigens and mAb CC-G33 were tested for ability to stain COS-7 cells transfected with cDNA encoding human CD14. Only mAb CC-G33 recognised the human molecule. The six mAbs were compared with mAb CC-G33 by flow cytometry and three were shown to be directed against bovine CD14.
Sopp
P
P
Institute for Animal Health, Compton Laboratory, Newbury, UK.
Kwong
L S
LS
Howard
C J
CJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D019556:COS Cells; D002417:Cattle; D005434:Flow Cytometry; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D014162:Transfection / Q000235:genetics
D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors