Whole blood production of thromboxane, prostacyclin and leukotriene B4 after dietary fish oil supplementation in man: effect of vitamin E.
12 subjects were given 30 ml/day of a fish oil already stabilized with vitamin E (1.5 IU/g) and other natural antioxidants (fish oil, FO), and the same fish oil supplemented with extra vitamin E (to total 4.5 IU/g) (FO+E), in a randomized double-blind cross-over study. The whole blood production of thromboxane B2, measured in serum, was reduced after 4 weeks of ingestion of both FO+E (by 47%, P < 0.01) and of FO (by 40%, P < 0.05) whereas 6-keto-PGF1 alpha increased slightly in both cases, by 4% and 5% respectively, both NS. Leukotriene B4 production decreased on both FO+E (by 20%, NS) and FO (by 17%, P < 0.05). This study thus showed that a stabilized fish oil had marked effects on eicosanoid production, which may be important for its cardiovascular effect. Further supplementation with vitamin E had no additional effect, indicating that the vitamin E content (1.5 IU/g) in this stabilized fish oil might have been optimal.
Department of Forensic Medicine, University of Uppsala, Sweden.
K
K
Engström
R
R
Luostarinen
T
T
Saldeen
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis / Q000097:blood; D000328:Adult; D018592:Cross-Over Studies; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004311:Double-Blind Method; D011464:Epoprostenol / Q000096:biosynthesis* / Q000097:blood; D005395:Fish Oils / Q000008:administration & dosage*; D006801:Humans; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013929:Thromboxane B2 / Q000096:biosynthesis* / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D007975:Leukotriene B4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol