High-performance liquid chromatographic determination of midazolam in rat brain.
A high-performance liquid chromatography method for the determination of midazolam in rat brain is described. Midazolam and the internal standard halazepam were extracted with toluene and analyzed isocratically on a reversed-phase column with a mobile phase consisting of methanol, acetonitrile and potassium phosphate buffer. Detection was monitored by ultraviolet absorption at 240 nm. The standard curves were linear over the range of 25-350 ng midazolam per 50 mg brain tissue. The day-to-day coefficient of variation ranged from 1.7 to 6.9%. The limit of quantification was 80 ng/g brain tissue. The method is rapid, simple and reproducible for brain analysis.
Neurology Service, Department of Veteran Affairs Medical Center, Los Angeles, CA 90073, USA.
Q
Q
Jiang
N Y
NY
Walton
S
S
Gunawan
D M
DM
Treiman
D000818:Animals; D014151:Anti-Anxiety Agents / Q000032:analysis / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D003975:Diazepam / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D007275:Injections, Intravenous; D016014:Linear Models; D008140:Lorazepam / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics; D008874:Midazolam / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics* / Q000627:therapeutic use; D051381:Rats; D015203:Reproducibility of Results; D013226:Status Epilepticus / Q000188:drug therapy
D014151:Anti-Anxiety Agents; D008140:Lorazepam; D003975:Diazepam; D008874:Midazolam