Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus.
We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Pyrococcus furiosus (Pf), a hyperthermophillic archeon. Sequence analysis of the Pf gene indicated an open reading frame specifying a protein of 485 amino acids (aa) with a calculated M(r) of 52900. Canonical Archaea promoter elements, Box A and Box B, are located -49 and -17 nucleotides (nt), respectively, upstream of the putative start codon. The sequence of the putative active-site region conforms to the IMPDH signature motif and contains a putative active-site cysteine. Phylogenetic relationships derived by using all available IMPDH sequences are consistent with trees developed for other molecules; they do not precisely resolve the history of Pf IMPDH but indicate a close similarity to bacterial IMPDH proteins. The phylogenetic analysis indicates that a gene duplication occurred prior to the division between rodents and humans, accounting for the Type I and II isoforms identified in mice and humans.
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439, USA.
F R
FR
Collart
J
J
Osipiuk
J
J
Trent
G J
GJ
Olsen
E
E
Huberman
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018387:Codon, Initiator; D005798:Genes, Bacterial; D006801:Humans; D007168:IMP Dehydrogenase / Q000235:genetics*; D007291:Inosine Monophosphate / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid
D018387:Codon, Initiator; D007291:Inosine Monophosphate; D007168:IMP Dehydrogenase