A p56(lck) ligand serves as a coactivator of an orphan nuclear hormone receptor.
Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, COUP-TFII stimulates transcription directly. A cellular factor, isolated by interaction cloning, bound COUP-TFII in vitro and allowed COUP-TFII to function as a transcriptional activator in mammalian cells. This factor is identical to a recently described ligand of the tyrosine kinase signaling molecule p56(lck), suggesting that it mediates cross-talk between mitogenic and nuclear hormone receptor signal transduction pathways.
Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. rrachubi@anat.med.ualberta.ca
S L
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Marcus
C J
CJ
Winrow
J P
JP
Capone
R A
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Rachubinski
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D002352:Carrier Proteins / Q000378:metabolism*; D002645:Chickens; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D006801:Humans; D017874:Immediate-Early Proteins; D008024:Ligands; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008322:Mammals; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011987:Receptors, Steroid; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D017384:Sequence Deletion; D000071456:Sequestosome-1 Protein; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D019061:src-Family Kinases / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D008024:Ligands; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D009838:Oligodeoxyribonucleotides; D011506:Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011994:Recombinant Proteins; D012097:Repressor Proteins; D000071456:Sequestosome-1 Protein; D015534:Trans-Activators; D014157:Transcription Factors; D019061:src-Family Kinases; C497588:Nr2f2 protein, rat; C099718:SQSTM1 protein, human; C095569:NR2F2 protein, human