The enterohemolysin phenotype of bovine Shiga-like toxin-producing Escherichia coli (SLTEC) is encoded by the EHEC-hemolysin gene.
Naturally occurring enterohemolysin negative variants were observed during studies on bovine Shiga-like toxin-producing E. coli (SLTEC). Examination of three strains (413/89-1 and 332, 026:H-, and 570/89, O111:H-) and their isogenic variants (413/89-6, 332-I and 570/89-I, respectively) showed, that in each strain loss of the enterohemolytic phenotype correlated with the loss of a large plasmid ranging from 94 to 104 kb in size. The hemolysin determinant present on the 94 kb plasmid of strain 413/89-1 was cloned and discovered by DNA and N-terminal aminoacid sequence analysis to be highly homologous to the recently published EHEC-hemolysin (HlyEHEC; Schmidt et al., 1994; 1995). When a recombinant plasmid harboring this determinant was reintroduced into the enterohemolysin negative isogenic mutant 413/89-6, the enterohemolytic phenotype was restored. Southern blot hybridization analysis was used to demonstrate that the HlyEHEC is plasmid-borne in SLTEC-strains. Our cumulative data suggest that the enterohemolytic phenotype of SLTEC is encoded by the plasmid-borne HlyEHEC. These results further demonstrate the close similarity between SLTEC-isolates from bovine and human.
Wieler
L H
LH
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Germany.
Tigges
M
M
Ebel
F
F
Schäferkordt
S
S
Djafari
S
S
Schlapp
T
T
Baljer
G
G
Chakraborty
T
T
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases; D017931:DNA Primers; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D014644:Genetic Variation; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
D001427:Bacterial Toxins; D017931:DNA Primers; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C066219:enterohemolysin