Intracerebroventricular administration of missense oligodeoxynucleotide induces interleukin-6 mRNA expression in brain and spleen of rats.
After intracerebroventricular (i.c.v.) injection of a missense oligodeoxynucleotide (MS-ODN) solution in rats, transcripts of the proinflammatory cytokine interleukin (IL)-6 were induced in hypothalamus, hippocampus, cortex and spleen and increased levels of circulating IL-6 and corticosterone were detected. Moreover, using a biotelemetric method body temperature of rats injected with ODN or Ringer solution was monitored over a period of 24 h after the injection. The i.c.v. injection of ODN induced a fever response which peaked at 6 h post-injection. These observations demonstrate that central administration of ODNs generates an inflammatory response in the central nervous system (CNS) and in the periphery as well.
Max Planck Institute of Psychiatry, Department of Neuroendocrinology, Munich, Germany.
G
G
Pezeshki
B
B
Schöbitz
T
T
Pohl
J M
JM
Reul
D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007276:Injections, Intraventricular; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*
D015850:Interleukin-6; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D003345:Corticosterone