Modulation of cardiac intermediate filament proteins in the chick heart by fibric acid derivatives.
The effects of different fibrates (bezafibrate, fenofibrate and gemfibrozil) on intermediate filaments were studied in cultured chick embryo heart cells after treatment for 6 or 24 h. Treatment led to alterations in total protein levels, as well as changes in protein levels, in the cytoplasmic and cytoskeletal fractions of cultured cells. Desmin was increased in the cytoskeletal fraction of all cultures after 6 h of treatment regardless of the drug tested, whereas vimentin was decreased in the cytoskeletal fraction only in cells treated with fenofibrate. These findings suggest that the alterations caused by fibrates in desmin and vimentin protein content may be related with the secondary effects that these drugs have on the cardiovascular system in patients treated with fibrates.
Department of Experimental Biology and Health Sciences, University of Jaen, Spain.
A E
AE
Aranega
M C
MC
Velez
F J
FJ
Gonzalez
C
C
Melguizo
J C
JC
Prados
A
A
Aranega
D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo / Q000187:drug effects* / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D011345:Fenofibrate / Q000494:pharmacology*; D015248:Gemfibrozil / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism
D007381:Intermediate Filament Proteins; D015248:Gemfibrozil; D011345:Fenofibrate; D001629:Bezafibrate