Effect of dexamethasone on insulin receptor mRNA levels, RNA stability and isotype RNA pattern in U-937 human promonocytic cells.
The administration of 5 x 10(-6) mol/l dexamethasone transiently increased insulin receptor (IR) mRNA levels in U-937 human promonocytic cells, which reached the maximum level at 15 h of treatment. Dexamethasone treatment did not affect the IR mRNA half-life (approximately 4 h), suggesting that the increase is regulated at the transcriptional level. The stimulatory action of dexamethasone was not prevented by the simultaneous presence of the protein synthesis inhibitor cycloheximide, indicating that the induction of IR gene transcription occurs as a direct response to the action of the synthetic glucocorticoid. Finally, the A isoform (lacking exon 11) was found to be the only IR isoform present in both untreated and dexamethasone treated-U-937 cells.
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
M A
MA
Leal
P
P
Aller
C
C
Calle
D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression; D005938:Glucocorticoids / Q000494:pharmacology*; D006207:Half-Life; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism*; D011972:Receptor, Insulin / Q000235:genetics*
D005938:Glucocorticoids; D012333:RNA, Messenger; D012313:RNA; D003907:Dexamethasone; D011972:Receptor, Insulin