Peripheral lymphoid tissue-like adhesion molecule expression in nodular infiltrates in inflammatory myopathies.
Non-granulomatous nodular accumulations of inflammatory cells in inflammatory myopathies were studied to characterize adhesion mechanisms used for leukocyte recruitment. The nodules had a B-cell-rich center surrounded by a helper T-cell-rich peripheral zone, resembling lymph nodes. The T-cell-rich zones harbored high-walled venules resembling high endothelial venules (HEV), whose endothelia frequently expressed ICAM-1, VCAM-1, and less constantly E-selectin. This endothelial adhesion molecule profile differs from that found in polymyositis, inclusion body myositis, or dermatomyositis, but resembles that in lymphoid tissues. Also, the peripheral lymph node addressin, a vascular addressin specific for peripheral lymphoid tissue HEV, was present on many HEV. This adhesion system is probably responsible for the excessive lymphocyte recruitment. The similar cellular organization and lymphocyte recirculation mechanisms of the nodular infiltrates in muscle and of lymph nodes suggest that the former may also produce antibodies.
University Hospital, Department of Neurology, Gent, Belgium.
J L
JL
De Bleecker
A G
AG
Engel
E C
EC
Butcher
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001065:Appendix / Q000737:chemistry / Q000473:pathology; D017252:CD3 Complex / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry; D019002:CD56 Antigen / Q000032:analysis / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000032:analysis*; D019040:E-Selectin / Q000032:analysis / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology; D037102:Lectins; D008198:Lymph Nodes / Q000737:chemistry* / Q000473:pathology; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000473:pathology; D009220:Myositis / Q000276:immunology* / Q000473:pathology*; D014066:Palatine Tonsil / Q000737:chemistry* / Q000473:pathology; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D051918:Sialic Acid Binding Ig-like Lectin 2; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; C497867:CD22 protein, human; D017252:CD3 Complex; D019002:CD56 Antigen; D015815:Cell Adhesion Molecules; D019040:E-Selectin; C069886:L-selectin counter-receptors; D037102:Lectins; D008565:Membrane Proteins; D016201:Receptors, Lymphocyte Homing; D051918:Sialic Acid Binding Ig-like Lectin 2; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1