Alpha 1-adrenergic receptors in the mammalian cochlea.
The presence of alpha 1-adrenergic receptors in the mammalian cochlea has previously been suggested by physiological experiments using antagonists specific to the receptor. However, the characteristics of adrenergic receptors in the cochlea have not been described. By employing [3H]-prazosin, high affinity, specific binding sites with characteristics of alpha 1-adrenergic receptors have now been identified and characterized in the chinchilla cochlea. Analysis of the specific [3H]-prazosin binding indicates that prazosin binds to a single class of high-affinity sites with a dissociation constant, kd, of 2.9 x 10(-9) M and a maximum number of binding sites, Bmax, of 30 fmol/mg dry tissue. Furthermore, the binding characteristics suggest that these receptors may be related to the microvasculature of the cochlea. These results provide a rational basis for the observed actions of alpha-adrenergic drugs on the auditory system.
Department of Otolaryngology-Head and Neck Surgery, Henry Ford Hospital, Detroit, MI, USA.
S Y
SY
Liu
D Z
DZ
Pitovski
B G
BG
Shivapuja
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D002682:Chinchilla; D003051:Cochlea / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D018340:Receptors, Adrenergic, alpha-1 / Q000187:drug effects / Q000378:metabolism*
D000317:Adrenergic alpha-Antagonists; D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin