Neurogranin is locally concentrated in rat cortical and hippocampal neurons.
The rat protein kinase C substrate neurogranin has a granular distribution in cortical and hippocampal neurons. We demonstrate that in these cells, granular labelling corresponds to a local concentration of neurogranin-immunoreactivity at both the membranes of mitochondria and trans-Golgi vesicles and soma-proximal dendritic shaft and spinal head structures. Our findings suggest that the function of neurogranin could be affected by protein assembly at these cellular sites.
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, C.N.R.S. Bât. CERVI, Hôpital de la Pitié Salpêtrière, Paris, France.
M
M
Neuner-Jehle
J P
JP
Denizot
J
J
Mallet
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002148:Calmodulin-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002540:Cerebral Cortex / Q000166:cytology*; D003712:Dendrites / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000737:chemistry; D006624:Hippocampus / Q000166:cytology*; D008297:Male; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D051579:Neurogranin; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism; D017966:Pyramidal Cells / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; C496651:Nrgn protein, rat; D051579:Neurogranin; D011493:Protein Kinase C