Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection.
A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.
Mweene
A S
AS
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
Ito
T
T
Okazaki
K
K
Ono
E
E
Shimizu
Y
Y
Kida
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000175:diagnosis*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits
D000914:Antibodies, Viral; D000956:Antigens, Viral