Structure of the O-specific polysaccharide from Burkholderia pickettii strain NCTC 11149.
A polymeric fraction (the putative O antigen) has been isolated from the lipopolysaccharide of the type strain of Burkholderia pickettii. The components of the polymer and their molar proportions were: L-rhamnose (3), 2-acetamido-2-deoxy-D-glucose (1), and O-acetyl (1). By means of NMR studies and chemical degradations, the basic repeating-unit of the polymer was identified as a linear tetrasaccharide of the structure shown. The O-acetyl group is probably located at position 2 of the 3-substituted alpha-L-Rha p. Similar polymers constitute O antigens in the related species Burkholderia solanacearum.
School of Chemistry, University of Hull, UK.
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Galbraith
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George
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Wyklicky
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Wilkinson
D000117:Acetylglucosamine / Q000032:analysis; D019117:Burkholderia / Q000378:metabolism*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D012210:Rhamnose / Q000032:analysis
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D012210:Rhamnose; D000117:Acetylglucosamine