A set of genetic markers for the chromosomes of the imperfect yeast Arxula adeninivorans.
The nuclear genome of the anamorphic yeast Arxula adeninivorans was analysed by benomyl-induced haploidization of parasexual hybrids marked with 32 auxotrophic mutations and pulsed field gel electrophoresis followed by DNA hybridization. Twenty-seven genes have been arranged into four linkage groups by haploidization, 15 genes belong to group 1, six to group 2, and three each to groups 3 and 4. Five genes could be localized by DNA hybridization on three out of four separated chromosomes. The gene LYS2 of the largest linkage group 1 and the 25S rDNA were identified on the largest chromosome, the GAA and the TEF1 gene on chromosome 2, and the ILV1 gene of linkage group 4 on the smallest chromosome.
Ernst-Moritz-Arndt-University, Institute of Genetics and Biochemistry, Greifswald, FRG.
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Samsonova
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Kunze
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Bode
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Böttcher
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005800:Genes, Fungal; D008040:Genetic Linkage; D005819:Genetic Markers; D008938:Mitosis; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015003:Yeasts / Q000235:genetics*
D005819:Genetic Markers