Enteroviral polymerase chain reaction in the investigation of aseptic meningitis.
We used a nested polymerase chain reaction (nPCR) to seek evidence for enteroviruses in clinical samples from patients with symptoms of aseptic meningitis. When compared with conventional virus isolation methods on a total of 366 samples collected during 1994-1995, an increase in positivity from 6% to 27% was shown. The results indicate that nPCR would be a valuable aid to the laboratory diagnosis of enteroviral infections as it can detect those enteroviruses that cannot be identified by current isolation methods.
Scottish Centre for Infection and Environmental Health, Ruchill Hospital, Glasgow, Scotland.
M H
MH
Riding
J
J
Stewart
G B
GB
Clements
D N
DN
Galbraith
D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008253:Macaca mulatta; D008297:Male; D008587:Meningitis, Viral / Q000821:virology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D004279:DNA, Viral