Expression of plasminogen activator inhibitor 2 in the adult and embryonic mouse tissues.
We have studied the expression of plasminogen activator inhibitor 2 (PAI-2) in the adult mouse and during embryonic development using immunohistochemistry and the polymerase-chain reaction. Mouse PAI-2 mRNA was mainly expressed in the skin, bone-marrow, spleen, lung, thymus, and urinary bladder. Immunohistochemical studies suggested that PAI-2 was synthesized in macrophages and epithelial cells such as skin epithelial cells, transitional cells of the urinary bladder, and mesothelial cell of peritoneum and pleura. PAI-2 mRNA and antigen expression was observed only in the skin of 15 day-old mouse embryos. These data suggest that PAI-2 may play a role in protecting the mouse embryo from the protease attack in the amniotic fluid and also in maintaining and/or protecting the structure of various surfaces in the mouse.
Institute of Hematology, Jichi Medical School, Japan.
Y
Y
Kawata
J
J
Mimuro
M
M
Kaneko
K
K
Shimada
Y
Y
Sakata
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D005314:Embryonic and Fetal Development / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D004926:Escherichia coli; D005260:Female; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017396:Plasminogen Activator Inhibitor 2 / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011994:Recombinant Proteins / Q000096:biosynthesis; D012867:Skin / Q000166:cytology / Q000196:embryology / Q000378:metabolism*
D017396:Plasminogen Activator Inhibitor 2; D011994:Recombinant Proteins