Characterization of the RNA dependent DNA polymerase of bovine leukemia virus.
The reverse transcriptase-RNA dependent DNA polymerase of Bovine Leukemia Virus (BLV) was isolated and characterized. The enzyme has a molecular weight of about 80kd and the isoelectric point is 7.6. The enzyme prefers magnesium, as a divalent cation using synthetic homopolymeric template primer poly (C) oligo (dG). Monoclonal antibodies directed against reverse transcriptase of human immunodeficiency virus type I (HIV-I) did not crossreact with the isolated polymerase.
Laboratory of Molecular Biology (ZBC), University Medical School, Frankfurt am Main, Germany.
I
I
Demirhan
V
V
Altanerova
A
A
Chandra
T
T
Gerber
D
D
Hofmann
C
C
Altaner
P
P
Chandra
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D054303:HIV Reverse Transcriptase / Q000276:immunology; D001909:Leukemia Virus, Bovine / Q000201:enzymology*; D008970:Molecular Weight; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase