Molecular properties of enzymes involved in diacylglycerol and phosphatidate metabolism.
Diacylglycerol and phosphatidate serve as key lipid intermediates of glycerolipid biosynthesis and also as the second messengers liberated in signal-stimulated cells. The molecular properties of enzymes involved in the metabolic processing of the two lipids are largely unknown. We have revealed by cDNA cloning the presence of a novel enzyme family of diacylglycerol kinase (DGK) having unique structural characteristics. Furthermore, we recently identified the plasma membrane-bound phosphatidate phosphatase (PAP) as a 35 kDa glycoprotein. Detailed studies on the two enzymes would contribute to understanding the molecular mechanisms underlying the control of both glycerolipid biosynthesis and signal transduction.
Department of Biochemistry, Sapporo Medical University School of Medicine, Japan.
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H
Kanoh
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Kai
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Wada
D000818:Animals; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism*; D006801:Humans; D010711:Phosphatidate Phosphatase / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015290:Second Messenger Systems; D015398:Signal Transduction
D004075:Diglycerides; D010743:Phospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; D010711:Phosphatidate Phosphatase