The Arabidopsis cyclin-dependent kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle.
Cell cycle progression is regulated by cyclin-dependent kinases (CDKs). Arabidopsis thaliana contains two cdk genes, cdc2aAt and cdc2bAt. This paper compares the developmental and cell cycle phase-dependent transcription of both cdk genes. In situ hybridizations revealed that cdc2bAt steady-state mRNAs, much like cdc2aAt, are found both in meristematic cells and cells with a high proliferative competence. Cdc2aAt is expressed in every meristematic cell whereas cdc2bAt is found to be expressed in a patchy pattern. An even smaller number of meristematic cells express the mitotic cyc1At. These data indicate that cdc2bAt and cyc1At mRNAs accumulate in a particular cell cycle phase in agreement with evidence provided by hybridization experiments of flow cytometrysorted nuclei and the use of cell cycle blockers on roots. The data indicate that cdc2bAt is preferentially expressed in S and G2 phases whereas cdc2aAt expression is constitutive throughout the cell cycle, as shown previously. The existence of two distinct CDK classes in plants is proposed: (i) constitutively expressed CDKs containing a PSTAIRE motif (e.g. cdc2aAt) and (ii) CDKs with divergent motifs which are expressed during a limited interval of the cell cycle (e.g. cdc2bAt).
Segers
G
G
Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.
Gadisseur
I
I
Bergounioux
C
C
de Almeida Engler
J
J
Jacqmard
A
A
Van Montagu
M
M
Inzé
D
D
D017360:Arabidopsis / Q000235:genetics*; D016203:CDC2 Protein Kinase / Q000096:biosynthesis* / Q000145:classification; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis*; D003624:Darkness; D005434:Flow Cytometry; D016195:G2 Phase / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D006131:Growth Inhibitors / Q000494:pharmacology; D017403:In Situ Hybridization; D007399:Interphase / Q000235:genetics*; D007527:Isoenzymes / Q000096:biosynthesis* / Q000145:classification; D008027:Light; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification; D018749:RNA, Plant / Q000302:isolation & purification; D016196:S Phase / Q000235:genetics; D014018:Tissue Distribution
D006131:Growth Inhibitors; D007527:Isoenzymes; D012333:RNA, Messenger; D018749:RNA, Plant; D016203:CDC2 Protein Kinase; D018844:Cyclin-Dependent Kinases