Proliferating cell nuclear antigen in neurones: induction by u.v. irradiation.
Proliferating cell nuclear antigen (PCNA) plays a pleiotropic role in DNA replication, excision repair of injured DNA and apoptosis. We found that PCNA protein is expressed predominantly in CA3 pyramidal neurones in hippocampal slices cultured from 1-week-old rats. Immunoblotting analysis revealed that this PCNA expression is enhanced by u.v. irradiation with no effect on neuronal viability. These results suggest that expression of PCNA in non-proliferating central neurones is associated with repair of injured DNA.
Kanzakigawa Laboratory, Shionogi Research Laboratories, Osaka, Japan.
M
M
Okada
T
T
Sakaguchi
K
K
Kawasaki
D000818:Animals; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism*; D051381:Rats; D014466:Ultraviolet Rays
D018809:Proliferating Cell Nuclear Antigen