32P-postlabeling of 1,3-butadiene and 4-vinyl-1-cyclohexene metabolite-DNA adducts: in vitro and in vivo applications.
Epoxides of 1,3-butadiene, i.e. 1,2-epoxy-3-butene and 1,2:3,4-diepoxybutane are DNA-reactive metabolites. No methods have been reported for detecting DNA adducts of these compounds in exposed animals or humans. The purpose of this study has been to develop a 32P-postlabeling assay for adducts of 1,3-butadiene and its dimer, 4-vinyl-1-cyclohexene. The assay was applied to skin DNA of mice exposed topically to diepoxides of these dienes. A dose-dependent increase in in vivo adduct formation was observed for both compounds. The newly developed assay will find applications in mechanistic studies on these and related compounds and in human biomonitoring.
Mabon
N
N
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA.
Randerath
K
K
D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D003510:Cyclohexanes / Q000378:metabolism*; D053138:Cyclohexenes; D018736:DNA Adducts / Q000032:analysis*; D004852:Epoxy Compounds / Q000378:metabolism*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D012867:Skin / Q000378:metabolism; D014753:Vinyl Compounds / Q000378:metabolism*
D002273:Carcinogens; D003510:Cyclohexanes; D053138:Cyclohexenes; D018736:DNA Adducts; D004852:Epoxy Compounds; D014753:Vinyl Compounds; C012606:4-vinyl-1-cyclohexene dioxide; C007366:diepoxybutane