Time-resolved fluorescence spectroscopy.
Time-resolved fluorescence spectroscopy is used to monitor molecular interactions and motions that occur in the picosecond-nanosecond time range, and is especially useful in the analysis of biomolecular structure and dynamics. Recent advances in the application of time-resolved fluorescence spectroscopy to biological systems have led to a better understanding of the origin of nonexponential fluorescence decay in proteins, the use of tryptophan analogs as unique spectroscopic probes of protein-protein interactions, the detailed characterization of protein-folding processes and intermediates, and the development of new approaches to the study of DNA-protein interactions.
Scripps Research Institute, Department of Molecular Biology, La Jolla, CA 92037, USA. millar@scripps.edu
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D000818:Animals; D006801:Humans; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence / Q000379:methods*