Modulation of A-currents by [K+]o in acutely isolated pyramidal neurones of juvenile rat entorhinal cortex and hippocampus.
Pyramidal neurones of the entorhinal cortex and of hippocampal areas CA1 and CA3 were acutely isolated from juvenile rats. The effect of lowering the concentration of extracellular potassium ions ([K+]o) on fast transient A-currents (IA) was studied using the whole-cell configuration of the patch-clamp technique. Upon lowering [K+]o from 5.4 to 0 mM amplitudes of IA in all pyramidal neurones were reduced only at test potentials positive to -20 mV, but were increased at potentials below -20 mV. Omission of magnesium ions from the intracellular perfusate resulted in an increase of IA in external K+-free solution at any potential tested. Moreover, when [K+]o was reduced steady-state activation and inactivation curves of IA were shifted in the hyperpolarizing direction.
Institut für Physiologie der Charité, Abt Neurophysiologie, Humboldt Universität zu Berlin, Germany.
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Eder
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Klee
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Heinemann
D000818:Animals; D018728:Entorhinal Cortex / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D011188:Potassium / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D015221:Potassium Channels; D011188:Potassium