Aging impairs regulation of intracellular pH in rat hippocampal slices.
We investigated whether aging changed H+ homeostasis in hippocampal slices bathed in HEPES buffer. Intracellular pH in hippocampal slices from rats aged 26-27 months (7.06 +/- 0.02) was significantly lower compared with that in slices from rats aged 6-7 months (7.16 +/- 0.04). Age did not influence extracellular ph. Age-related reductions in intracellular pH may reflect altered pH regulation that potentially affects brain function and could contribute to the increased vulnerability of the aged brain to metabolic stress.
Department of Neurology, University of Miami, School of Medicine, FL 33136, USA.
E L
EL
Roberts
T J
TJ
Sick
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001578:Benzopyrans; D005453:Fluorescence; D006624:Hippocampus / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012235:Rhodamines / Q000378:metabolism
D001578:Benzopyrans; D009284:Naphthols; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D006859:Hydrogen