Sphingolipid-derived signalling modulators: interaction with phosphatidylserine.
We previously described the synthesis of two deuterium-labelled sphingoid bases, which made it possible to perform NMR spectroscopy on this family of signalling modulators in membranes (Rigby, A.C, Barber, K.R and Grant, C.W.M. (1995) Biochim. Biophys. Acta 1240, 75-82). In the present work we sought to test the concept that such mediators may display altered physical behaviour through association with anionic lipids - as a possible mechanism of involvement in signal transduction. Lyso-dihydrogalactosylceramide with deuterium nuclei at C4 and C5 of the sphingosine backbone and at C'3 and C'4 of the galactose ring ([2H4]lyso-GalCer), and N,N-dimethylsphingosine with deuterated amino-methyl groups ([2H6]dimethylsphingosine), were assembled as minor components into unsonicated fluid bilayer membranes of 1-palmitoyl-2-oleoylphosphatidylcholine/cholesterol. The effect of (anionic) phosphatidylserine was considered in this zwitterionic host matrix. The results present a picture of rapidly reversible interaction. The (-) charged phosphatidylserine exerted readily-measurable control over the orientation of the carbohydrate residue of [2H4]lyso-GalCer. In contrast, surrounding (-) charges exerted little spectral influence at the level of C4 and C5 of the lyso-GalCer, membrane-inserted, backbone; or at the level of the amino group of dimethylsphingosine. It would appear that packing alterations induced by the phosphatidylserine/sphingoid base association can translate into sizeable spatial constraints in the neighbouring aqueous domain.
Department of Biochemistry, University of Western Ontario, London, Canada.
A C
AC
Rigby
K R
KR
Barber
C W
CW
Grant
D002784:Cholesterol; D003903:Deuterium; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy; D008962:Models, Theoretical; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000138:chemical synthesis / Q000737:chemistry*; D015398:Signal Transduction; D013107:Sphingolipids / Q000138:chemical synthesis / Q000737:chemistry*; D013329:Structure-Activity Relationship
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D013107:Sphingolipids; D002784:Cholesterol; D003903:Deuterium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine