Biodistribution of trace elements in normal, aluminum-overloaded and cadmium-overloaded mice. An application of the radioactive multitracer technique.
The radioactive multitracer technique was applied to simultaneous evaluation of biobehavior of many trace elements in normal, Al-overloaded and Cd-overloaded mice. The tissue and subcellular distributions of Be, Sc, V, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Y, Zr, Ru, and Rh were examined by using a multitracer solution, prepared from an Ag target irradiated with heavy ions, in 9 organs (brain, cardiac muscle, lung, liver, spleen, pancreas, kidney, skeletal muscle, and bone) and blood. Their tissue and subcellular distributions were evaluated by gamma-ray spectrometry in terms of tissue uptake rate (the radioactivity percentage of injected dose per gram of tissue, percent of dose/g) and fractionation percentage (the radioactivity percentage of nuclear fraction, mitochondrial fraction, microsomal fraction or supernatant fraction, percent), respectively. Comparative biobehavior of these elements in brain and liver is reported. Aluminium- and Cd-induced synergistic interactions were observed among these trace elements in their liver uptake behavior.
School of Health Sciences, Faculty of Medicine, Kanazawa University, Ishikawa, Japan.
R
R
Amano
S
S
Oishi
S
S
Enomoto
F
F
Ambe
D000535:Aluminum / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism*; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D005720:Gamma Rays; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008861:Microsomes / Q000737:chemistry; D008928:Mitochondria / Q000737:chemistry; D011849:Radioactive Tracers; D014131:Trace Elements / Q000378:metabolism* / Q000633:toxicity
D011849:Radioactive Tracers; D014131:Trace Elements; D002104:Cadmium; D000535:Aluminum