Mouse multivalent IgG(2a, b) antibody--ricin A-chain immunotoxins combined with homologous or heterologous interleukin 2 in the treatment of a murine malignant lymphoproliferation (EL4).
Two immunotoxins containing ricin A-chain, staphylococcal protein A and mouse anti-Thy 1.2 antibodies of IgG(2a,b) subclass, were prepared. The two multivalent immunotoxins of 750 and 370 kDa and molar ratio A-chain: IgG of 1:2, were used for the treatment of mice bearing ascitic EL4 lymphoma. The immunotoxin-treatment, performed intraperitoneally, was combined or not with homologous or heterologous interleukin 2. The antitumor effects expressed by increase of mice survival time (p < 0.001 as compared with nontreated animals) corresponded to a proportion of 88-90% lymphoma cell-kill by immunotoxin and 93-95% by combination treatment (immunotoxin + interleukin 2), as calculated from the relationship between the survival time of nontreated mice and the number of tumor cells inoculated.
Mota
G
G
Center of Immunology, Stefan Nicolau Institute of Virology, Bucharest, Romania.
Marches
R
R
Cialacu
V
V
Roman
V
V
Kozma
E
E
Mărgineanu
M
M
Dima
S
S
Moraru
I I
II
D000818:Animals; D004357:Drug Synergism; D007074:Immunoglobulin G / Q000145:classification* / Q000627:therapeutic use*; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D000922:Immunotoxins / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000145:classification / Q000627:therapeutic use*; D007945:Leukemia, Lymphoid / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D012276:Ricin / Q000627:therapeutic use*; D013205:Staphylococcal Protein A / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D000922:Immunotoxins; D007376:Interleukin-2; D013205:Staphylococcal Protein A; D012276:Ricin