Tumor-associated antigen 90K activates myelomonocytic cell line THP-1.
90K is a tumor-associated antigen. Using myelomonocytic cell line THP-1 we determined neopterin production and tryptophan degradation after exposure of cells to 90K in the presence and the absence of interferon-gamma. Interferon-gamma is a well known stimulus for THP-1 cells inducing e.g. neopterin production and tryptophan degradation. Treatment of cells with 50 micrograms/ml 90K induced significant neopterin formation, and the exposure of cells to 90K in addition to 100 U/ml interferon-gamma amplified neopterin production compared to the sole effect of interferon-gamma. In parallel, a significant degradation of tryptophan was observed in culture supernatants leading to the formation of kynurenine. When the cells were treated with the combination of 90K and interferon-gamma the degradation of tryptophan was further enhanced. The data demonstrate that tumor-associated antigen 90K interferes with immunocompetent target cells and is able to induce a biochemical response in monocytic cells.
Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
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Altindag
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Marth
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Werner-Felmayer
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Natoli
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Zeimet
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Wachter
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Iacobelli
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Fuchs
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001708:Biopterins / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002352:Carrier Proteins; D002460:Cell Line; D005260:Female; D006023:Glycoproteins; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008074:Lipoproteins / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000494:pharmacology*; D019798:Neopterin; D014364:Tryptophan / Q000378:metabolism*; D016326:Extracellular Matrix Proteins
D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001708:Biopterins; D002352:Carrier Proteins; D006023:Glycoproteins; D007371:Interferon-gamma; D008074:Lipoproteins; D009363:Neoplasm Proteins; D019798:Neopterin; D014364:Tryptophan; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins