Reactivity of workshop antibodies with a non-ruminant species: crossreactivity with canine blood leukocytes.
Three-hundred and eight monoclonal antibodies detecting surface determinants on ruminant cells were tested on canine leukocytes by flow cytometry. Thirty mabs showed crossreactivity: 25 of them reacted uniformly with cells of three unrelated dogs, five displayed a polymorphic reaction pattern. Thus, in addition to mabs with yet unknown specificity, antibodies to ruminant WC9, B-cell antigens and possible CD29, CD49d cross-react with canine cells, and probably react with the homologous canine antigen.
Immunology Unit, School of Veterinary Medicine, Hannover, Germany. jschub@immunologie.tiho-hannover.de
S
S
H-Joachim
A
A
Beer
H U
HU
Rabe
W
W
Leibold
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification* / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004285:Dogs / Q000276:immunology*; D005434:Flow Cytometry; D007962:Leukocytes / Q000276:immunology*; D012418:Ruminants
D000911:Antibodies, Monoclonal