Granulocyte-macrophage colony stimulating factor promotes prolonged survival and the support of virulent infection by African swine fever virus of macrophages generated from porcine bone marrow and blood.
Long-surviving cultures of non-adherent cells of the monocyte-macrophage lineage were established from the bone marrow and blood of weanling pigs by culturing cells from these tissues in the presence of recombinant porcine granulocyte-macrophage colony stimulating factor (GM-CSF). The cells increased in number, principally during the first 4 weeks of culture, bound monoclonal antibodies recognizing porcine macrophage antigens and avidly phagocytosed latex particles. The GM-CSF generated mononuclear phagocytes were highly infectible [correction of infectable] with a virulent Malawi isolate of African swine fever virus (ASFV) and able to generate levels of virus progeny similar to those produced by freshly isolated pig macrophages. The cultured cells retained their susceptibility to ASFV infection for as long as the cultures survived i.e. for up to 3 months.
BBSRC Institute of Animal Health, Pribright Laboratory, Surrey, UK. sylvia.denham@bbsrc.ac.uk
S
S
Denham
S M
SM
Brookes
G H
GH
Hutchings
R M
RM
Parkhouse
D000358:African Swine Fever Virus / Q000254:growth & development / Q000472:pathogenicity*; D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D010587:Phagocytosis; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013552:Swine; D014774:Virulence
D003470:Culture Media; D011993:Recombinant Fusion Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor