Vitamin D3 elicits calcium response and activates blood monocyte-derived macrophages from patients with vitamin D dependent rickets type II.
We studied the effects of vitamin D3 metabolites on intracellular free Ca2+ concentration ([Ca2+]i) and the respiratory burst of monocyte-derived macrophages (MDM) from patients with vitamin D dependent rickets type II. Treatment of MDM from the patients and healthy donors with 1 nM 1,25(OH)2D3 produced a rapid elevation of [Ca2+]i and similarly primed both types of cells for enhanced capacity for O2- release with phorbol diester. These results suggest that macrophages may have distinct non-genomic pathways of vitamin D3, which partly explain the absence of immunodeficiency and the disappearance of rickets after treatment with vitamin D3 in the patients.
Department of Clinical Nutrition, School of Medicine, The University of Tokushima, Tokushima City, Japan.
E
E
Takeda
K
K
Takata
H
H
Yamanaka
Y
Y
Taketani
K
K
Morita
K
K
Miyamoto
M
M
Shono
S
S
Teshima
K
K
Rokutan
D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002112:Calcifediol / Q000494:pharmacology; D002117:Calcitriol / Q000031:analogs & derivatives / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D012279:Rickets / Q000097:blood / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D015650:24,25-Dihydroxyvitamin D 3; D000255:Adenosine Triphosphate; D002117:Calcitriol; C051883:maxacalcitol; D002112:Calcifediol; D002118:Calcium