Evaluation of ribotyping as epidemiologic tool for typing Escherichia coli serogroup O157 isolates.
A total of 121 representative Escherichia coli O157:H7 and O157:NM (nonmotile) isolates were characterized by ribotype, phage type, verotoxin genotype, and genomic fingerprints generated by pulsed-field gel electrophoresis. Ribotyping was not able to discriminate between O157:H7 isolates, and phage typing and pulsed-field gel electrophoresis were the most valuable and discriminatory techniques.
Martin
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IE
Bureau of Microbiology, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
Tyler
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SD
Tyler
K D
KD
Khakhria
R
R
Johnson
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WM
D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000145:classification*; D006801:Humans; D012703:Serotyping