Ribonuclease P from plant nuclei and photosynthetic organelles.
RNase P consists of both protein and RNA subunits in all organisms and organelles investigated so far, with the exception of chloroplasts and plant nuclei where no enzyme-associated RNA has been detected to date. Studies on substrate specificity revealed that cleavage by plant nuclear RNase P is critically dependent on a complete and intact structure of the substrate. No clearcut answer is yet possible regarding the order of processing events at the 5' or 3' end of tRNAs in the case of nuclear or chloroplast processing enzymes. RNase P from a phylogenetically ancient photosynthetic organelle will be discussed in greater detail: The enzyme from the Cyanophora paradoxa cyanelle is the first RNase P from a photosynthetic organelle which has been shown to contain an essential RNA subunit. This RNA is strikingly similar to its counterpart from cyanobacteria, yet it lacks catalytic activity. Properties of the holoenzyme suggest an intermediate position in RNA enzyme evolution, with an Eukaryotic-type, inactive RNA and a prokaryotic-type small protein subunit. The possible presence of an RNA component in RNase P from plant nuclei and modern chloroplasts will be discussed, including a critical evaluation of some criteria that have been frequently applied to elucidate the subunit composition of RNase P from different organisms.
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
A
A
Schön
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology*; D016384:Consensus Sequence; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism*; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015388:Organelles / Q000201:enzymology*; D010788:Photosynthesis; D010802:Phylogeny; D010944:Plants / Q000201:enzymology*; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D043262:Ribonuclease P; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
D012322:RNA Precursors; D016337:RNA, Catalytic; D012261:Ribonucleoproteins; D004722:Endoribonucleases; C468368:RPP14 protein, human; D043262:Ribonuclease P