Expression of high-affinity IgE receptor (Fc epsilon RI) on human alveolar macrophages from atopic and non-atopic patients.
The expression of high-affinity IgE receptor (Fc epsilon RI) on alveolar macrophages was examined in order to determine the association of alveolar macrophages with IgE-dependent inflammation. Immunostaining showed that a 15-1 monoclonal antibody against Fc epsilon RI alpha chain reacted with alveolar macrophages from both atopic and non-atopic patients by immunostaining. In addition, preincubation of 15-1 monoclonal antibody specifically blocked monomeric IgE binding to alveolar macrophages. Reverse transcription polymerase chain reaction showed that CD14+ alveolar macrophages expressed Fc epsilon RI alpha, beta and gamma chain mRNA. These results show that alveolar macrophages express Fc epsilon RI and may participate in IgE-dependent inflammation mediated by Fc epsilon RI.
Department of Internal Medicine, Toho University School of Medicine, Sakura Hospital, Japan.
K
K
Ochiai
M
M
Kagami
K
K
Umemiya
R
R
Matsumura
T
T
Kawashima
H
H
Tomioka
D001992:Bronchoalveolar Lavage Fluid; D002277:Carcinoma / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism*; D012507:Sarcoidosis / Q000378:metabolism
D012333:RNA, Messenger; D017455:Receptors, IgE