Participation of water in Hin recombinase--DNA recognition.
The participation of water molecules in the interaction between the Hin recombinase and its operator DNA has been detected by analysis of the dissociation constant in the presence of varying concentrations of neutral solutes and cosolvents. The dissociation constant as measured by gel mobility shift assays increased as the concentration of dimethyl sulfoxide, glycerol, sucrose, or polyethylene glycol was increased. Osmotic pressure is the only property that correlates with the change in the dissociation constant for all compounds. This data indicates that binding of a small population of water molecules accompanies formation of the Hin-DNA complex, and points to a novel role for solvent molecules in assisting site specific interaction between DNA-binding proteins and their cognate DNA sequence.
Department of Biochemistry, University of Illinois at Urbana-Champaign 61801, USA.
C R
CR
Robinson
S G
SG
Sligar
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D009997:Osmotic Pressure; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D012045:Regulatory Sequences, Nucleic Acid; D012997:Solvents / Q000494:pharmacology*; D013379:Substrate Specificity; D013395:Sucrose / Q000494:pharmacology; D014867:Water / Q000494:pharmacology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012997:Solvents; D014867:Water; D011092:Polyethylene Glycols; D013395:Sucrose; D004254:DNA Nucleotidyltransferases; C059997:Hin recombinase; D005990:Glycerol; D004121:Dimethyl Sulfoxide