NotI jumping and linking clones as a tool for genome mapping and analysis of chromosome rearrangements in different tumors.
Long-range restriction site maps are of central importance for mapping the human genome. The use of clones from linking and jumping libraries for genome mapping offers a promising alternative to the laborious procedures used up until now. In the present review, this research field is analyzed with particular emphasis on the implementation of a shot-gun sequencing strategy for genome mapping and the use of NotI linking clones for analysis of rearrangements in tumors and tumor cell lines.
Zabarovsky
E R
ER
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Kashuba
V I
VI
Gizatullin
R Z
RZ
Winberg
G
G
Zabarovska
V I
VI
Erlandsson
R
R
Domninsky
D A
DA
Bannikov
V M
VM
Pokrovskaya
E
E
Kholodnyuk
I
I
Petrov
N
N
Zakharyev
V M
VM
Kisselev
L L
LL
Klein
G
G
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D015321:Gene Rearrangement; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases