PCR analysis of polymorphisms at the D13S25 locus.
Evidence that the D13S25 locus lies close to a potential tumour suppressor gene implicated in the pathogenesis of B-CLL has been based on detection of LOH and bi-allelic loss using the pH2-42 probe. The SspI polymorphism detected by this probe has been identified by sequencing adjacent clones and a polymorphic (TA)n repeat has been found. Amplification of the region encompassing both polymorphic markers by PCR increases the informativity to 80%.
Molecular Biology, Pathology Department, Bournemouth General Hospital, UK.
R E
RE
Ibbotson
R M
RM
Chapman
M M
MM
Corcoran
D G
DG
Oscier
D000483:Alleles; D014408:Biomarkers, Tumor; D002882:Chromosomes, Human, Pair 13; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D014408:Biomarkers, Tumor