Isolation and characterization of the Erwinia herbicola bio operon and the sequences of the bioA and bioB genes.
The biotin operon of Erwinia herbicola was cloned and characterized. The operon consists of five genes arranged in the order, bioABFCD. The operon is negatively regulated via the interaction of a proposed biotin repressor with an operator sequence that lies between the bioA and bioB genes. The nucleotide sequences of bioA (7,8-diaminopelargonic acid transferase), bioB (biotin synthetase) and the regulatory region were determined and analyzed. The deduced amino acid sequences of bioA and bioB are also aligned with currently available homologs to obtain the UPGMA (unweighted pair group method with arithmetic mean) evolutionary tree.
Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
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Wu
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Chen
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Shiuan
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001710:Biotin / Q000235:genetics*; D003001:Cloning, Molecular; D004885:Erwinia / Q000235:genetics*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D010802:Phylogeny; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013466:Sulfurtransferases; D000637:Transaminases
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D001710:Biotin; D000637:Transaminases; C102100:BioA protein, bacteria; D013466:Sulfurtransferases; C087072:biotin synthase, E coli