cDNA cloning and characterization of the human interleukin 13 receptor alpha chain.
We have cloned cDNAs corresponding to the human interleukin 13 receptor alpha chain (IL-13Ralpha). The protein has 76% homology to murine IL-13Ralpha, with 95% amino acid identity in the cytoplasmic domain. Only weak IL-13 binding activity was found in cells transfected with only IL-13Ralpha; however, the combination of both IL-13Ralpha and IL-4Ralpha resulted in substantial binding activity, with a Kd of approximately 400 pM, indicating that both chains are essential components of the IL-13 receptor. Whereas IL-13Ralpha serves as an alternative accessory protein to the common cytokine receptor gamma chain (gammac) for IL-4 signaling, it could not replace the function of gammac in allowing enhanced IL-2 binding activity. Nevertheless, the overall size and length of the cytoplasmic domain of IL-13Ralpha and gammac are similar, and like gammac, IL-13Ralpha is located on chromosome X.
Laboratory of Molecular Immunology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-1674, USA.
M J
MJ
Aman
N
N
Tayebi
N I
NI
Obiri
R K
RK
Puri
W S
WS
Modi
W J
WJ
Leonard
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D006822:Hybrid Cells; D018793:Interleukin-13 / Q000378:metabolism; D053661:Interleukin-13 Receptor alpha1 Subunit; D007457:Iodine Radioisotopes; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D018123:Receptors, Interleukin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D053660:Receptors, Interleukin-13; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C508181:IL13RA1 protein, human; C508183:Il13ra1 protein, mouse; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D007457:Iodine Radioisotopes; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13