Immunocytochemical analysis of gangliosides in rat primary cerebellar cultures using specific monoclonal antibodies.
We studied the expression of ganglioside antigens in primary cultures of rat cerebellum using an immunocytochemical technique with mouse monoclonal antibodies (MAbs) specific for various gangliosides. Twelve MAbs that specifically recognize each ganglioside were used. Our study revealed that there is a cell type-specific expression of ganglioside antigens in the primary cultures. A number of b-series gangliosides were detected in the granule cells, whereas a-series gangliosides were not intensely expressed. GD1b was detected in the granule cells. GD2 appeared to be present in a subset of the granule cells or a type of small neurons. GD3 was associated not only with the granule cells, but also with both astrocytes and oligodendrocytes. An O-Ac-disialoganglioside, which was suggested to be O-Ac-LD1, was restrictedly detected in Purkinje cells. The other gangliosides were not detected clearly in these cells. These results suggest that several gangliosides may be useful markers for identifying cells in primary cultures of the rat cerebellum; particularly b-series gangliosides such as GD2 and GD1b for the granule cells and O-Ac-LD1 for Purkinje cells.
Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.
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Kawashima
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Nagata
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Tai
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D002772:Cholera Toxin; D002855:Chromatography, Thin Layer; D019084:Fluorescent Antibody Technique, Indirect; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009439:Neuraminidase; D009474:Neurons / Q000166:cytology*; D011689:Purkinje Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013744:Tetanus Toxin; D014357:Trypsin
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D013744:Tetanus Toxin; D002772:Cholera Toxin; D009439:Neuraminidase; D014357:Trypsin