Interleukin-13 and interleukin-4 act as interleukin-6 inducers in human microglial cells.
The effect of interleukin 4 (IL-4) and IL-13 on IL-6 and IL-1 beta production by human embryonic microglial cells and human peripheral blood monocyte cells (PBMC) was compared. IL-4 or IL-13 increase intra-cellular IL-6 mRNA and IL-6 titres in supernatants of microglial cells whereas they decreased IL-6 production by PBMC tested in the same conditions. IL-4 and IL-13 increased also IL-1 beta production by human microglial cells. Stimulations associating IL-4 or IL-13 and IL-1 beta had an additive effect on IL-6 production by microglial cells, whereas, in the same conditions, an inhibitory effect was observed in PBMC. In contrast, dexamethasone downregulated IL-6 production by microglial cells and PBMC. Finally, IL-1 receptor (IL-1r) type 1 and IL-1r type 2 were detected on human microglial cells but it was demonstrated that IL-13 and IL-4 acted as IL-6 inducers in human microglial cells independently of the IL-1/IL-1r pathway.
Sébire
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Laboratoire Virus, Neurone et Immunité, UFR Kremlin-Bicêtre, Université Paris XI, France.
Delfraissy
J F
JF
Demotes-Mainard
J
J
Oteifeh
A
A
Emilie
D
D
Tardieu
M
M
D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D018793:Interleukin-13 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D017628:Microglia / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*
D007375:Interleukin-1; D018793:Interleukin-13; D015850:Interleukin-6; D015847:Interleukin-4