Dilated intercellular spaces: a morphological feature of acid reflux--damaged human esophageal epithelium.
OBJECTIVE
Dilated intercellular spaces are a sign of epithelial damage in acid-perfused rabbit esophagus, a change best identified by transmission electron microscopy. The aim of this study was to determine if this change is also a feature of acid damage to human esophageal epithelium.


METHODS
Endoscopic esophageal biopsy specimens from patients with (n = 11) and without (n = 13) recurrent heartburn were examined using transmission electron microscopy. Of 11 patients with heartburn, 6 had erosive esophagitis and 5 had normal-appearing mucosa on endoscopy; 13 controls had no symptoms or signs of esophageal disease. Using a computer, intercellular space diameter was measured from transmission electron microscopy photomicrographs of the specimen from each patient.


RESULTS
Intercellular space diameter was significantly greater in specimens from patients with heartburn than those from controls; this was true irrespective of whether the patient had erosive or nonerosive disease. Space diameters of > or = 2.4 microns were present in 8 of 11 patients with heartburn and in no controls.


CONCLUSIONS
Dilated intercellular spaces are a feature of reflux damage to human esophageal epithelium. As a morphological marker of increased paracellular permeability, its presence in patients without endoscopic abnormalities may help explain their development of heartburn.
Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana, USA.
N A
NA
Tobey
J L
JL
Carson
R A
RA
Alkiek
R C
RC
Orlando
D000328:Adult; D000368:Aged; D004848:Epithelium / Q000473:pathology / Q000648:ultrastructure; D004947:Esophagus / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D005764:Gastroesophageal Reflux / Q000208:ethnology / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged