Cytotoxicity of two new ribosome-inactivating proteins, cinnamomin and camphorin, to carcinoma cells.
Cinnamomin (two-chain) and camphorin (single-chain), two novel ribosome-inactivating proteins (RIPs) purified from the seeds of Cinnamomum camphora, produced inhibitory effects in cultured carcinoma cells. The IC50 of cinnamomin to the human hepatocarcinoma cell-line 7721 and the melanoma cell-line M21 were 18.8 nmol and 11.7 nmol respectively. The IC50 of camphorin to the human hepatocarcinoma cell-line 7721 was 59 nmol, whereas the melanoma cell-line M21 was not susceptible to camphorin. Furthermore, cinnamomin exhibited a remarkable inhibitory effect on the growth of solid melanoma in the skin of the nude mouse. An R-fragment could be isolated from ribosomes of cinnamomin- or camphorin-treated carcinoma cells after incubation with acidic aniline, indicating that the cytotoxicity of these two new RIPs to carcinoma cells might result from modification to the ribosomes.
Shanghai Institute of Biochemistry, Academia Sinica, China.
J
J
Ling
W Y
WY
Liu
D020418:Algal Proteins; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D004278:DNA, Superhelical / Q000378:metabolism; D004198:Disease Susceptibility; D005656:Fungal Proteins / Q000494:pharmacology*; D006801:Humans; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008819:Mice, Nude; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D011506:Proteins; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes; D014407:Tumor Cells, Cultured
D020418:Algal Proteins; D000970:Antineoplastic Agents; D004278:DNA, Superhelical; D005656:Fungal Proteins; D010940:Plant Proteins; D011506:Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C104406:camphorin protein, Cinnamomum camphora; C061930:cinnamomin, Phytophthora cinnamomi; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins