B cell hybridoma presents both B-cell and T-cell epitopes for stimulating antibody production via CD23 pathway.
CD23+ B cell hybridoma 17A11, pulsed with IgE:TNP-KLH triggered IgA, IgG, and IgE antibody production via CD23-mediated presentation. Prior anti-CD23 treatment abrogated 95% of the humoral antibody responses. Both B and T cell epitopes were presented by 17A11 B cell epitopes as recognized by IgG but not T cell epitopes were sensitive to treatment with 0.2 M acetic acid. Efficacy of antigen presentation via CD23 on 17A11 was comparable to that mediated via surface immunoglobulins (sIg) on a CD23 negative 4.5 parental fusion partner B cell line. This is the first demonstration that IgE:TNP-KLH pulsed B cell hybridomas present both B- and T-cell epitopes in stimulating IgA, IgG, and IgE antibody production, and raise a pertinent issue whether IgE antibodies produced under pathophysiological conditions may serve as positive feedback signal for sustaining production of different classes of antibodies.
Schmaltz
R
R
Department of Veterinary Science, IANR, University of Nebraska-Lincoln, USA.
Wang
Y Y
YY
Kung
Q X
QX
Liu
F T
FT
Petro
T
T
Chen
S S
SS
D000818:Animals; D000917:Antibody Formation; D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D017455:Receptors, IgE / Q000502:physiology*
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D011947:Receptors, Antigen, B-Cell; D017455:Receptors, IgE