Immune response to progesterone involved in Cu(2+)-mediated polyanion-protein complex--antigen specificity and affinity of hybridoma clones.
The immunogenic properties of water soluble (PAA-Cu(2+)-BSA) and colloidal (PAA-Cu(2+)-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and or by the representation of dormant determinants existing in the miner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu(2+)-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities Kd ranging between 1.3 x 10 (-5) and 9 x 10(-8) M.
M
M
Mustafaev
F
F
Yücel
B
B
Cirakoğlu
E
E
Bermek
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000915:Antibody Affinity; D000918:Antibody Specificity; D000941:Antigens; D002417:Cattle; D003300:Copper / Q000276:immunology*; D000939:Epitopes; D005620:Freund's Adjuvant / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D011374:Progesterone / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D003300:Copper; D000939:Epitopes; D005620:Freund's Adjuvant; D000071228:Polyelectrolytes; D011108:Polymers; D011374:Progesterone; D011506:Proteins; D012710:Serum Albumin, Bovine