Platelet activation studies with anti-CD41/61 monoclonal antibodies.
The purpose of this study was to monitor platelet activation by following alpha IIb beta 3 integrin (GpIIb/IIIa complex or CD41/CD61) on the platelet surface by flow cytometry (FCM) analysis using workshop mAbs. The results obtained with the mAbs showed increased expression of the GpIIb/IIIa complex (about 40%) on the platelet membrane surface under thrombin stimulation.
Mateo
A
A
Facultad de Veterinaria, Universidad de Cordoba, Departamento de Genetica, Spain.
Perez de la Lastra
J M
JM
Garrido
J J
JJ
Llanes
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology; D005434:Flow Cytometry; D039661:Integrin beta3; D016023:Integrins / Q000096:biosynthesis / Q000276:immunology; D015539:Platelet Activation / Q000187:drug effects* / Q000276:immunology*; D010974:Platelet Aggregation / Q000276:immunology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis / Q000276:immunology*; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology*; D012756:Sheep; D013917:Thrombin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039661:Integrin beta3; D016023:Integrins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D013917:Thrombin