[Immunodetection of vascular endothelial growth factor in the vitreous of eyes with proliferative diabetic retinopathy].
To determine the association of a hypoxia-induced, vascular endothelial cell selective growth factor: vascular endothelial growth factor (VEGF) on proliferative diabetic retinopathy (PDR), undiluted vitreous fluids were collected from 10 eyes with PDR at the time of vitrectomy. Vitreous fluids from 10 eyes without PDR were used as control. All samples were electrophoresed with a 14% sodium dodesyl sulfatepolyacrylamide gel, and electrically transferred to a Durapore membrane. Immunoblot detection of VEGF was done by standard western blotting using rabbit polyclonal anti-human VEGF antibody. Positive immunoreaction bands were observed on lanes of 7 samples from PDR, and they were comigrated with recombinant human VEGF. No reactions were observed on lanes of all samples without PDR. The presence of VEGF within the vitreous fluids of eyes with PDR was determined, and suggesting the association of VEGF with PDR.
Department of Ophthalmology, Fukuoka University, Japan.
H
H
Ozaki
H
H
Hayashi
K
K
Oshima
Y
Y
Imanaga
M
M
Kuroki
F
F
Arakawa
M
M
Kuwahara
Y
Y
Matsuoka
D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D003930:Diabetic Retinopathy / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis*; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014822:Vitreous Body / Q000737:chemistry*
D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors