Comparison of fungal ornithine decarboxylases.
We designed PCR primers by comparison of the deduced amino acid sequences of several ornithine decarboxylase (ODC) genes. They were used to amplify fragments homologous to these genes from several dimorphic fungi. These were sequenced and the deduced amino acid sequences were compared with the corresponding regions of ODCs from different sources. Fungal ODCs fell into a compact group, well separated from the ODCs of other taxa. Sequence homology among fungal enzymes corresponded to their taxonomic position. Interesting patterns of amino acid conservation in ODCs from fungi, distinct from other organisms, were detected.
Departamento de Ingeniería Genética (Unidad Irapuato), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 629, Irapuato Gto. 36500, Mexico.
J C
JC
Torres-Guzman
B
B
Xoconostle-Cazares
L
L
Guevara-Olvera
L
L
Ortiz
G
G
San-Blas
A
A
Dominguez
J
J
Ruiz-Herrera
D000595:Amino Acid Sequence; D005658:Fungi / Q000201:enzymology*; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction
D009955:Ornithine Decarboxylase