Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family.
We identified and cloned a cellular mediator of herpes simplex virus (HSV) entry. Hamster and swine cells resistant to viral entry became susceptible upon expression of a human cDNA encoding this protein, designated HVEM (for herpesvirus entry mediator). HVEM was shown to mediate the entry of several wild-type HSV strains of both serotypes. Anti-HVEM antibodies and a soluble hybrid protein containing the HVEM ectodomain inhibited HVEM-dependent infection but not virus binding to cells. Mutations in the HSV envelope glycoprotein gD significantly reduced HVEM-mediated entry. The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family.
Northwestern University Medical School, Department of Microbiology-Immunology, Chicago, Illinois 60611, USA.
R I
RI
Montgomery
M S
MS
Warner
B J
BJ
Lum
P G
PG
Spear
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D018139:Simplexvirus / Q000502:physiology*; D013552:Swine; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology; D014162:Transfection; D014709:Vero Cells / Q000378:metabolism / Q000821:virology
D018076:DNA, Complementary; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human