VIP-antiserum and indomethacin inhibit calcium and bicarbonate secretion by the inflamed feline gallbladder mucosa.
Ionized calcium (Ca2+) and bicarbonate (HCO(3)-) are important ions for gallstone formation. In the present study the effect of indomethacin and vasoactive intestinal peptide-antiserum (VIP-antiserum) on the Ca2+, HCO(3)- and fluid secretion in the inflamed gallbladder were tested in a validated experimental model in cats. The animals were studied in acute experiments 6 weeks after cystic duct ligation and gallstone implantation. During basal conditions there was a continuous secretion of Ca2+, HCO(3)- and fluid into the lumen of the inflamed gallbladder. Indomethacin and VIP-antiserum inhibited the Ca2+, HCO(3)- and fluid secretion across the inflamed gallbladder mucosa. Intraluminal amiloride inhibited the absorption of Ca2+, HCO(3)- and fluid after indomethacin infusion. An impaired absorption of Ca2+, HCO(3)- and fluid in the inflamed gallbladder is probably important for the solubility of calcium salts in the early stages of inflammation in the gallbladder mucosa.
Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
B
B
Nilsson
D
D
Delbro
A
A
Thune
S
S
Friman
L
L
Jivegård
J
J
Svanvik
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002415:Cats; D005260:Female; D005704:Gallbladder / Q000187:drug effects*; D007106:Immune Sera / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D001639:Bicarbonates; D007106:Immune Sera; D014660:Vasoactive Intestinal Peptide; D002118:Calcium; D007213:Indomethacin