The use of transposable P-elements of Drosophila melanogaster for introductory genetics laboratory courses.
Laboratories in introductory genetics courses often use Drosophila melanogaster as a tool for the students to understand how genes are transmitted and mapped. We demonstrate three advantages of using P-element containing inserts with the wild-type alleles of the genes to be mapped. First, inserts can be positioned at many places in the genome with relative ease; second, stocks can be chosen which have excellent viability; and third, inserts can be selected for precision in mapping with regard to marker genes. The results of a preliminary test with a beginning genetics laboratory are presented. The inserts exhibit excellent Mendelian ratios in F2 and backcross progeny and were reproducibly mapped by different student groups.
Morcillo
P
P
Section of Genetics and Development, Cornell University, Ithaca, New York, USA.
Tuttle
R
R
MacIntyre
R J
RJ
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005823:Genetics / Q000193:education; D008297:Male; D016254:Mutagenesis, Insertional; D010865:Pilot Projects
D004251:DNA Transposable Elements