Binding assay of low density lipoprotein to Asp-hemolysin from Aspergillus fumigatus.
Binding activity of Asp-hemolysin to low density lipoprotein (LDL) was measured. LDL binds to Asp-hemolysin with an affinity as high as the LDL receptor. The apparent Kd, determined by Scatchard plot analysis, was 8.9 x 10(-9) M [125I]LDL. Asp-hemolysin is thus identified as a novel LDL-binding protein.
Japanese Red Cross Sendai Hospital, Japan.
Y
Y
Fukuchi
Y
Y
Kudo
T
T
Kumagai
K
K
Ebina
K
K
Yokota
D001232:Aspergillus fumigatus; D005656:Fungal Proteins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*
C089497:Asp-HS protein, Aspergillus fumigatus; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D008077:Lipoproteins, LDL