Direct excision of 50 kb pair DNA fragments from megabase-sized fragments produced during apoptotic cleavage of genomic DNA.
The DNA of U937 cells exposed to two different apoptotic stimuli, namely the cocktail H2O2/3-aminobenzamide (3AB) and etoposide, was analyzed using field inversion gel electrophoresis (FIGE) as well as programmable, autonomously controlled electrode electrophoresis (PACE). The results obtained indicate that FIGE is not appropriate for sizing apoptotic DNA fragments. PACE appears to be more accurate and reliable and the results obtained with this technique strongly suggest that the 50 kb DNA fragments are directly excised from Mb-sized DNA fragments without the intermediate cleavage of 200-300 kb products.
Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Italy.
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D017209:Apoptosis; D001549:Benzamides / Q000494:pharmacology; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D053938:DNA Fragmentation; D004586:Electrophoresis / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D001549:Benzamides; D005047:Etoposide; C025160:3-aminobenzamide; D004247:DNA; D006861:Hydrogen Peroxide