Human UDP-galactose translocator: molecular cloning of a complementary DNA that complements the genetic defect of a mutant cell line deficient in UDP-galactose translocator.
We have cloned a cDNA that codes for a putative human UDP-galactose translocator (UGT) protein. The cDNA contained an open reading frame of 1,179 base pairs encoding a novel protein of 393 amino acids. Introduction of the open reading frame sequence into a UGT-deficient mouse cell line, Had-1, complemented the genetic defect of the mutant, namely the inability to transport UDP-galactose from the cytosol to the Golgi lumen, as judged from the lectin-sensitivity spectrum of the transformant. To our knowledge, this is the first mammalian nucleotide-sugar translocator whose cDNA sequence has been described.
Department of Physiological Chemistry, Tokyo Metropolitan Institute of Medical Science (Rinshoken).
N
N
Miura
N
N
Ishida
M
M
Hoshino
M
M
Yamauchi
T
T
Hara
D
D
Ayusawa
M
M
Kawakita
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D017173:Caenorhabditis elegans / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005816:Genetic Complementation Test; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014531:Uridine Diphosphate Galactose / Q000378:metabolism*; D000099237:UDP-Galactose Translocators
D002352:Carrier Proteins; D018076:DNA, Complementary; D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D014531:Uridine Diphosphate Galactose; D000099237:UDP-Galactose Translocators