Is urine leukocyte esterase test a useful screening method to predict Chlamydia trachomatis infection in women?
We evaluated the use of the leukocyte esterase test (LET) on first-catch urine specimens from women as a screening test to predict infection with Chlamydia trachomatis. For diagnosis, we used Abbott's ligase chain reaction (LCR) on urine specimens and isolation by tissue culture (TC) on cervical brushes. Of 4,053 women attending sexually transmitted disease and family planning clinics, 4.3% (n = 174) were positive by TC and 5.9% (n = 239) were positive by LCR. When LET was compared to TC, the sensitivity, specificity, positive predictive value, and negative predictive value were 54.0, 67.0, 6.8, and 97.0%, respectively. The corresponding performance of LET versus LCR was 53.1, 67.3, 10.1, and 95.8%. Almost half of the laboratory-confirmed chlamydial infections were negative by LET. The low specificity probably reflects multiple causes of pyuria in women and results in a low positive predictive value. LET is neither sensitive nor specific as a predictor of chlamydial infection and cannot be recommended for use as a screening test for C. trachomatis with first-catch urine samples from females from low- or moderate-prevalence populations.
University of California, San Francisco, USA.
J M
JM
Chow
J
J
Moncada
D
D
Brooks
G
G
Bolan
H
H
Shaw
J
J
Schachter
D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis; D004950:Esterases / Q000652:urine*; D005260:Female; D006801:Humans; D009504:Neutrophils / Q000201:enzymology*; D012680:Sensitivity and Specificity
D004950:Esterases