Detection of erythromycin-resistant determinants by PCR.
Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.
Department of Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA. Joyce_A_Sutcliffe@groton.pfizer.com
J
J
Sutcliffe
T
T
Grebe
A
A
Tait-Kamradt
L
L
Wondrack
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics*; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004917:Erythromycin