Na+-dependent release of Mg2+ from an intracellular pool in rat sublingual mucous acini.
Muscarinic stimulation induces release of Mg2+ from an intracellular pool in rat sublingual mucous acini (Zhang, G. H., and Melvin, J. E. (1992) J. Biol. Chem. 267, 20721-20727). In the present study we examined the interdependence of Mg2+ mobilization on intracellular Na+ and Ca2+ by monitoring the intracellular free concentrations of Na+ ([Na+]i), Mg2+ ([Mg2+]i), and Ca2+ ([Ca2+]i) using ion-sensitive fluorescent indicators. Gramicidin increased the intracellular concentrations of all three ions. Comparable to agonist-stimulated mobilization of Mg2+, the gramicidin-induced [Mg2+]i increase was independent of extracellular Mg2+ indicating release of Mg2+ from an intracellular pool. Clamping the [Ca2+]i near 30 nM with the Ca2+-selective chelator BAPTA failed to alter the [Na+]i or [Mg2+]i increases generated by gramicidin. In contrast, depletion of intracellular Na+ markedly suppressed the muscarinic-stimulated [Mg2+]i increase, whereas the [Ca2+]i increase was similar to that seen in physiological extracellular Na+. These results revealed that intracellular Mg2+ mobilization did not directly relate to the [Ca2+]i, but required an increase in [Na+]i. Consistent with this hypothesis, increasing [Na+]i by activating Na+ influx via the Na+/H+ exchanger also increased the [Mg2+]i. The Na+/Mg2+ exchange inhibitor quinidine suppressed both the gramicidin- and muscarinic-induced discharge of internal Mg2+. These results suggest that release of Mg2+ from an intracellular pool is mediated by a Na+-dependent Mg2+ transport mechanism in salivary acinar cells.
Department of Dental Research, University of Rochester, Rochester, New York 14642, USA. melvin@medinfo.rochester.edu
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D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D006096:Gramicidin / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism; D018721:Muscarinic Agonists / Q000494:pharmacology; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism*; D013361:Sublingual Gland / Q000187:drug effects / Q000378:metabolism*
D018721:Muscarinic Agonists; D006096:Gramicidin; D002217:Carbachol; D012964:Sodium; D008274:Magnesium; D011802:Quinidine