Non-virally mediated gene transfer into human central nervous system precursor cells.
Lipofectamine-based transfection was used as a method of choice to deliver the bacterial beta-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. beta-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.
Cattaneo
E
E
Institute of Pharmacological Sciences, University of Milan, Italy. cattaneo@isfunix.farma.unimi.it
Conti
L
L
Gritti
A
A
Frolichsthal
P
P
Govoni
S
S
Vescovi
A
A
D002411:Cation Exchange Resins; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D006801:Humans; D007202:Indicators and Reagents; D007763:Lac Operon; D008055:Lipids; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis; D013234:Stem Cells / Q000378:metabolism*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D002411:Cation Exchange Resins; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D011994:Recombinant Proteins; D001616:beta-Galactosidase