Protection against peroxynitrite-dependent tyrosine nitration and alpha 1-antiproteinase inactivation by ascorbic acid. A comparison with other biological antioxidants.
Peroxynitrite, formed by reaction of superoxide and nitric oxide, appears to be an important tissue-damaging species generated at sites of inflammation. In this paper, we compare the abilities of several biological antioxidants to protect against peroxynitrite-dependent inactivation of alpha 1-antiproteinase, and to inhibit tyrosine nitration upon addition of peroxynitrite. GSH and ascorbate protected efficiently in both systems. Uric acid inhibited tyrosine nitration but not alpha 1-antiproteinase inactivation. The possibility that ascorbic acid is an important scavenger of reactive nitrogen species in vivo is discussed.
Neurodegenerative Disease Research Centre, King's College, London, UK.
M
M
Whiteman
B
B
Halliwell
D000975:Antioxidants / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D009566:Nitrates / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D013582:Synovial Fluid / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; D009566:Nitrates; D000515:alpha 1-Antitrypsin; C039642:peroxynitric acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D010196:Pancreatic Elastase; D001205:Ascorbic Acid