Acrosome membrane associated protein kinase activity in hamster spermatozoa.
Acrosomal membranes isolated from the caput and cauda epididymal spermatozoa of hamster exhibited protein kinase activity and the endogenous protein substrates that were phosphorylated in the acrosomal membranes of caput and cauda spermatozoa were not all the same. The kinase activity was identified as a cAMP independent type and the use of specific stimulators and inhibitors indicated that the activity was not due to casein kinase, protein kinase A or protein kinase C but due to a tyrosine specific protein kinase that was not inhibited by Genistein. Phosphotyrosine was identified as the predominant phosphorylated residue in the proteins.
Devi
K U
KU
Centre for Cellular and Molecular Biology, Hyderabad, India.
Ahmad
M B
MB
Shivaji
S
S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000177:Acrosome / Q000201:enzymology*; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004822:Epididymis; D019833:Genistein; D006493:Heparin / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D007529:Isoflavones; D008565:Membrane Proteins; D019000:Phosphotyrosine; D013096:Spermine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D006493:Heparin; D019833:Genistein; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D013095:Spermidine