Activation and analysis of cryptic crt genes for carotenoid biosynthesis from Streptomyces griseus.
Genes encoding enzymes with sequence similarity to carotenoid biosynthetic enzymes of other organisms were cloned from Streptomyces griseus JA3933 and transformed into the colourless (non-daunorubicin producing) mutant Streptomyces griseus IMET JA3933/956/2. Cells harbouring these genes showed an orange-red pigmentation, caused by the strongly hydrophobic, membrane-bound lycopene. The cloned fragment (9 kb) contained seven genes, four transcribed in one direction (crtEIBV) and three (crtYTU) transcribed convergently to them. Three of these genes encode polypeptides that resemble geranylgeranyl-pyrophosphate (GGPP) synthases (CrtE), phytoene synthases (PS) (CrtB) and phytoene dehydrogenases (PDH) (CrtI), respectively, of various bacteria. These enzymes are sufficient for the formation of lycopene. crtE alone was sufficient to induce zeaxanthin formation in an Escherichia coli clone containing the crt gene cluster from Erwinia herbicola deleted for crtE. The combination of crtE and crtB led to formation of phytoene in S. griseus. The putative crtEp promoter region was cloned and mapped by primer extension analysis. In a gel retardation experiment, this fragment was specifically shifted by an unknown protein. CrtY shows similarity to lycopene cyclases that convert lycopene into beta-carotene, CrtT resembles various methyltransferases and CrtU a dehydrogenase. We conclude that these genes are functionally intact, but not expressed (cryptic) in the wild-type S. griseus strain.
Department of Cell and Molecular Biology, Hans Knöll Institute for Natural Product Research, Germany.
G
G
Schumann
H
H
Nürnberger
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G
Sandmann
H
H
Krügel
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002338:Carotenoids / Q000096:biosynthesis*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D019753:Intramolecular Lyases; D007535:Isomerases / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002338:Carotenoids; D007535:Isomerases; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase