Liver specific kynurenine(alanine):glyoxylate aminotransferase was expressed in kidney cell line.
Kynurenine (Alanine); glyoxylate aminotransferase (AGT) expression plasmid in the COS-7 was constructed. pGV-C was used as a expression vector which contains SV-40 promoter and enhancer. pGV-C and human AGT clone, H1-2, were digested by Hind III/Sma I separately. The AGT fragment was inserted into the digested pGV-C large fragment, The constructed plasmid was named as pGV-AGT. The constructed plasmid was transfected to COS-7 cultured cell by electroporation. The best electroporation condition was checked.
Minatogawa
Y
Y
Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
Kawai
C
C
Hatada
S
S
Sato
M
M
D000410:Alanine Transaminase / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors; D000637:Transaminases; D014162:Transfection
D011994:Recombinant Proteins; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase