Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics.
A wide range of approaches is available to parasitologists to aid in specific parasite identification and to formulate phylogenetic relationships. This review emphasises the usefulness of molecular genetic techniques, especially DNA-based procedures, in addressing problems of identification, characterisation and phylogeny of parasites. It should be stressed that an understanding of the various DNA approaches, techniques and target genes most likely to be effective in addressing key issues in diagnostic parasitology and systematics is still developing. Nevertheless, DNA methods clearly have great potential with regard to specificity and sensitivity, and applications will increase further with technological advance. Indeed, because of the minimal requirements for material, PCR-based methods especially should prove of immense value in future studies with parasites.
McManus
D P
DP
Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Brisbane, Australia.
Bowles
J
J
D000818:Animals; D018509:DNA, Helminth / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D019143:Evolution, Molecular; D017238:Genes, Helminth; D017125:Genes, Protozoan; D005821:Genetic Techniques; D010271:Parasites / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D018509:DNA, Helminth; D016054:DNA, Protozoan