Identification and quantification of Tuber melanosporum Vitt. sterols.
The sterol composition of Tuber melanosporum was examined by medium-pressure liquid and high-performance liquid chromatography, nuclear magnetic resonance spectroscopy, and mass spectrometry. Ergosterol (ergosta-5,7,22-trienol) and brassicasterol (ergosta-5,22-dienol) were identified as the major components (90%). Their quantities and their relative concentrations, probably good indicators for differentiating mature truffles from immature colored ones, were determined during the maturation of the fungus. The results show that the total quantity of sterols decreased with the age of the fungus, but that the ergosterol/brassicasterol ratio remained relatively constant.
Laboratoire de Chimie Agro-industrielle, Ecole Nationale Supérieure de Chimie, Toulouse, France.
E
E
Harki
A
A
Klaebe
T
T
Talou
R
R
Dargent
D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D002775:Cholestadienols / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004875:Ergosterol / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010840:Phytosterols; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification
D002775:Cholestadienols; D010840:Phytosterols; D013261:Sterols; C003532:brassicasterol; D004875:Ergosterol