The activation status of ovine CD45R+ and CD45R- efferent lymph T cells after orf virus reinfection.
The dynamics and activation status of CD4+ and CD8+ T-cells differentially expressing the CD45R (220 kDa) antigen were studied in prefemoral efferent lymph draining the site of cutaneous reinfection with orf virus. CD4+, CD45R+ lymphoblasts preceded CD4+, CD45R- lymphoblasts during the first 48 h after reinfection. Thereafter, the output of both total and blast-transformed CD4+, CD45R- T-cells increased in proportion to the CD4+, CD45R+ cells for the duration of the virus reinfection. Output of CD8+, CD45R+ T-cells exceeded that of the CD8+, CD45R+ cells both before and after reinfection. However, within the lymphoblast population, CD8+, CD45R+ and CD8+, CD45R- T-cells increased and decreased in parallel. CD4+, CD45R- and CD8+, CD45R- T-cells produced interleukin-2, interferon-gamma and granulocyte-macrophage colony-stimulating factor after culture for 24 h without exogenous restimulation, whereas CD4+, CD45R+ T-cells produced only interleukin-2. The results show that although both CD45R+ and CD45R- alpha beta receptor+ T-cell subsets are activated as a consequence of virus reinfection in vivo, it is the CD45R- subset that predominates in the later stages of reinfection and is the principal cellular source of lymphokines in the efferent lymph.
Haig
D M
DM
Moredun Research Institute, Edinburgh, UK.
Deane
D L
DL
Myatt
N
N
Thomson
J
J
Entrican
G
G
Rothel
J
J
Reid
H W
HW
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000821:virology*; D002465:Cell Movement / Q000276:immunology; D004474:Ecthyma, Contagious / Q000209:etiology* / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000379:methods; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008196:Lymph / Q000166:cytology* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D009923:Orf virus / Q000472:pathogenicity*; D011971:Receptors, Immunologic / Q000276:immunology*; D012756:Sheep
D011971:Receptors, Immunologic; D017493:Leukocyte Common Antigens