Rapid chromosome detection in human gametes, zygotes, and preimplantation embryos using the PRINS technique.
OBJECTIVE
The analysis of the chromosomal constitution of human gametes and embryos is of particular importance for investigation of aneuploidy occurrence and diagnostic purposes. The PRINS method constitutes an alternative to FISH for in situ chromosomal identification. We have adapted this method to human gametes, zygotes, and preimplantation embryos.


RESULTS
Chromosome-specific labeling was obtained in gametes, zygotes, and isolated blastomeres. Simultaneous detection of two or three chromosomes can be completed in less than 3 hr using fluorochrome-labeled nucleotides.


CONCLUSIONS
The PRINS technique appears to be more efficient than FISH for detection and discrimination of alpha-satellite DNA sequences. The present study demonstrates the usefulness of PRINS for chromosomal screening and preimplantation diagnosis.
CNRS UPR 9008, Montpellier, France.
F
F
Pellestor
A
A
Girardet
G
G
Lefort
B
B
Andréo
J P
JP
Charlieu
D000782:Aneuploidy; D001755:Blastocyst / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000235:genetics; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010063:Ovum / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D019914:Taq Polymerase; D015053:Zygote / Q000648:ultrastructure*
D017931:DNA Primers; D004276:DNA, Satellite; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase