Localization of three novel zinc finger genes to the centromeric region of human chromosome 10 by fluorescence in situ hybridization.
An oligonucleotide probe for the consensus sequence of the linker region of zinc finger proteins was used to isolate cDNA clones from a human fetal heart cDNA library. Following DNA sequencing analysis and comparison, genes for the novel clones were mapped by fluorescence in situ hybridization. We report the chromosomal localization of three zinc finger-coding genes to the region of centromere on human chromosome 10p11.1q-11.2, indicating involvement in gene duplication and chromosome rearrangement during primate evolution.
Department of Clinical Biochemistry, Toronto Hospital, Ontario, Canada.
R
R
Wang
E
E
Cukerman
H H
HH
Heng
C C
CC
Liew
D000595:Amino Acid Sequence; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016335:Zinc Fingers / Q000235:genetics*