[cGMP synthesis induced by atrial natriuretic peptide, C-type natriuretic peptide, and nitric oxide in the rat retina].
This study was undertaken to determine whether atrial natriuretic peptide (ANP)-, C-type natriuretic peptide (CNP)-, and nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathways exist in the rat retina. Exposure of the retina to ANP (10(-7)M), CNP (10(-7)M), S-nitroso-N-acetylpenicillamine (10(-5)M, SNAP; a NO doner), A23187 (10(-5)M; a Ca2+ionophore), and carbachol (10(-5)M) caused 1.45-1.67 fold increases in cGMP content (p < 0.01). Carboxy-PTIO (10(-3)M; a NO scavanger) blocked the increase in cGMP induced by A23187. Both carboxy-PTIO (10(-3)M) and NG-nitro-L-arginine (10(-3)M, L-NNA; a NO inhibitor) blocked the increase in cGMP induced by carbachol. Atropine (10(-5)M; a muscarinic receptor antagonist) also blocked the cGMP increase induced by carbachol. These data demonstrate that ANP-, CNP- and NO-cGMP pathways exist in the retina and that the NO-cGMP pathway may be linked to the activation of the muscarinic receptor.
Department of Ophthalmology, Tottori University, Japan.
Y
Y
Moriwaki
Y
Y
Kamisaki
T
T
Itoh
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001285:Atropine / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D006152:Cyclic GMP / Q000096:biosynthesis*; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D020098:Natriuretic Peptide, C-Type; D009569:Nitric Oxide / Q000494:pharmacology*; D019335:Nitroarginine / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000378:metabolism*; D026423:S-Nitroso-N-Acetylpenicillamine
D001565:Benzoates; D007093:Imidazoles; D007476:Ionophores; D011506:Proteins; D020098:Natriuretic Peptide, C-Type; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D019335:Nitroarginine; D009569:Nitric Oxide; D000001:Calcimycin; D026423:S-Nitroso-N-Acetylpenicillamine; D001285:Atropine; D009320:Atrial Natriuretic Factor; D002217:Carbachol; D010396:Penicillamine; D006152:Cyclic GMP