Effect of amino acid peroxides on the erythrocyte.
The effect of amino acid peroxides, relatively stable products of irradiation of amino acid solutions, on erythrocyte components was studied. Interaction of proline, lysine, valine, and leucine peroxides (100-300 mu M) with erythrocyte membranes brought about a decrease of membrane protein -SH group content and of activities of (Na+, K+)-ATPase and Ca2+ -ATPase, and induced aggregation of membrane proteins, due mainly to the formation of interpeptide disulfides. Interaction of amino acid peroxides with hemoglobin brought about hemoglobin oxidation to methemoglobin. The effects of amino acid peroxides are similar to those of t-butyl hydroperoxide. These results indicate that peroxides of amino acid and proteins, which can also be formed under physiological conditions, may be mediators of the cellular action of reactive oxygen species.
Department of Molecular Biophysics, University of Lodz, Poland.
M
M
Soszynski
A
A
Filipiak
G
G
Bartosz
J M
JM
Gebicki
D000596:Amino Acids / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005720:Gamma Rays; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000378:metabolism; D020122:tert-Butylhydroperoxide
D000596:Amino Acids; D006454:Hemoglobins; D008565:Membrane Proteins; D010545:Peroxides; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase