An assessment of nonspecific adsorption to Eudragit S-100 during affinity precipitation.
The problem of nonspecific adsorption to the reversibly soluble-insoluble polymers is of considerable importance in the design of an affinity precipitation protocol. It was seen that activation and coupling of the affinity ligand to the polymer changes the nature of the polymer surface in a significant fashion. The results with pure trypsin, partially purified trypsin preparation, and crude protein extract-containing protein inhibitors of trypsin and alpha-amylase and the reversibly soluble-insoluble polymer Eudragit S-100, show that nonspecific adsorption may not be a severe limitation in such systems.
Chemistry Department, Indian Institute of Technology, Delhi, India.
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Kumar
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Gupta
D000180:Acrylic Resins / Q000737:chemistry*; D002846:Chromatography, Affinity; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids; D014357:Trypsin / Q000302:isolation & purification*; D014361:Trypsin Inhibitors / Q000302:isolation & purification*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000180:Acrylic Resins; D011108:Polymers; D011109:Polymethacrylic Acids; D014361:Trypsin Inhibitors; C038300:methylmethacrylate-methacrylic acid copolymer; D000516:alpha-Amylases; D014357:Trypsin