Fecal fat measurement in the presence of long- and medium-chain triglycerides and fatty acids. Comparison of three methods.
BACKGROUND
It has been suggested that some of the limitations of the Van de Kamer method for fecal fat measurement could be overcome with the Jeejeebhoy method or the near-infrared reflectance assay.


METHODS
To test this hypothesis, a fecal fat test was carried out with the three methods, adding butter or MCT oil to the diet of four steatorrhoic patients. An in vitro recovery study of long- and medium-chain triglycerides was also performed.


RESULTS
The Jeejeebhoy method measured long- and medium-chain fats more accurately than the Van de Kamer method. It found consistently higher steatorrhea values. Mean results of the near-infrared reflectance analysis resembled those of the Van de Kamer method, but with wide discordance of individual data.


CONCLUSIONS
The Jeejeebhoy method is more accurate than the Van de Kamer method for fecal fat measurement. The difference may be clinically relevant when most fecal fatty acids derive from medium-chain triglycerides. Near-infrared reflectance may be a viable proposition only when a greater degree of approximation is acceptable.
Caliari
S
S
Division of Gastroenterologic Rehabilitation, University of Verona, Italy.
Vantini
I
I
Sembenini
C
C
Gregori
B
B
Carnielli
V
V
Benini
L
L
D002446:Celiac Disease / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008722:Methods; D014280:Triglycerides / Q000032:analysis*
D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides