Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer.
To develop a controlled release system for bone morphogenetic protein (BMP), poly(DL-lactide-co-glycolide) (PLGA) capsules containing BMP were prepared by an interfacial precipitation method using a water-in-oil-in-water emulsion. The surface morphology, particle size distribution, and hydrolytic degradation rate of the PLGA capsules were examined. The encapsulation yield and release rate of BMP in vitro were measured using fluorescein isothiocyanate-labeled BMP. The amount of BMP released from PLGA capsules increased between days 3 and 5. In addition, the effectiveness of BMP encapsulated in PLGA to induce bone formation in vivo was also examined by subcutaneous implantation in rats. Complete digestion of the capsules and new bone formation including bone marrow were identified by histologic examination of harvested tissues at 3 weeks after implantation. These results demonstrated that encapsulation of BMP with PLGA could be a promising method to induce bone in clinics.
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
M
M
Isobe
Y
Y
Yamazaki
S
S
Oida
K
K
Ishihara
N
N
Nakabayashi
T
T
Amagasa
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D002417:Cattle; D004337:Drug Carriers; D004339:Drug Compounding; D004343:Drug Implants; D006868:Hydrolysis; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D004343:Drug Implants; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid