The easy formation of antisperm antibodies in prepubertal boys and the difficult humoral response in severe-combined immunodeficiency mice.
OBJECTIVE
To investigate and compare mechanisms controlling the development of antisperm antibodies in situations with different status of active immunosuppression.


METHODS
Antibody activity against human sperm was analyzed by immunosorbent assays (ELISA) in sera samples of prepubertal boys and in circulation of sensitized immunocompromised mice.


METHODS
Procedures were performed in a university laboratory.


METHODS
Prepubertal healthy boys and patients with testicular failures.


METHODS
Blood was collected from healthy individuals and from patients with diagnosed testicular pathology; antisperm antibodies were rechecked after surgical intervention (orchidopexia or removal of the pathological gonad).


METHODS
Antisperm antibodies in quantitative ELISA.


RESULTS
Significant antibody activity to finally differentiated human sperm was detected in sera samples from prepubertal boys with testicular failures (cryptorchid or mobile testis), especially in individuals with both pathological gonads. "Naive" human lymphocytes deposited to peritoneal cavity of immunocompromised mice did not respond to in situ challenge with sperm antigens.


CONCLUSIONS
High antisperm antibody levels in prepubertal boys may suggest low immunosuppressive activity at this age. This situation may influence future fertility status of these individuals. Immunocompromised mice effectively prevented humoral response to sperm antigens suggesting different mechanism than T suppressor cell activity.
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
M
M
Kurpisz
M
M
Kasprzak
I
I
Mazurkiewicz
D000293:Adolescent; D000818:Animals; D000941:Antigens / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003456:Cryptorchidism / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011627:Puberty; D013094:Spermatozoa / Q000276:immunology*; D013733:Testicular Diseases / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies