A mechanism for regulation of melanoma invasion. Ligation of alpha6beta1 integrin by laminin G peptides.
Invasion of LOX human melanoma cells involves extracellular matrix (ECM) degradation and formation of cell surface invadopodia. Here we show that the ligation of alpha6beta1 by two peptides derived from the COOH-terminal globular domain of laminin-1 alpha1 chain (laminin G peptides), designated AG-10 (NPWHSIYITRFG) and AG-32 (TWYKIAFQRNRK), and antibodies against alpha6 and beta1 integrins promoted invasiveness. AG-10 and AG-32 inhibited cell adhesion on laminin, and the antibodies blocked cell adhesion on immobilized AG-10 and AG-32, suggesting that the peptides interact primarily with alpha6beta1 integrin. These soluble peptides and integrin antibodies induced invasiveness by causing an 2-3-fold increase in ECM degradation and invadopodial activity independently of adhesion activity of integrins that were prebound to ECM. The induced ECM degradation and invasion was associated with an increased surface expression of the 170-kDa membrane-bound gelatinase, seprase, as well as its intense localization at invadopodia but not at focal adhesions. However, the total expression levels of seprase, gelatinase A and beta1 integrins were not altered. We suggest that laminin G peptides act on the alpha6beta1 integrin signaling of invasion by stimulating invadopodial activities, which is distinct from their direct effects on cell adhesion on immobilized ECM.
Lombardi Cancer Center and Department of Cell Biology, Georgetown University Medical Center, Washington, D. C. 20007, USA. chenw@gunet.georgetown.edu
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Nakahara
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Nomizu
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Akiyama
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Yamada
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Yeh
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Chen
D000595:Amino Acid Sequence; D000906:Antibodies / Q000494:pharmacology; D014408:Biomarkers, Tumor; D002448:Cell Adhesion; D002460:Cell Line; D010450:Endopeptidases; D018093:Gelatinases / Q000096:biosynthesis; D006801:Humans; D039121:Integrin alpha6beta1; D016023:Integrins / Q000096:biosynthesis / Q000502:physiology*; D007700:Kinetics; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2; D008545:Melanoma / Q000473:pathology* / Q000503:physiopathology; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000096:biosynthesis; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D012697:Serine Endopeptidases; D014407:Tumor Cells, Cultured; D000099167:Fibroblast Activation Protein Alpha
D000906:Antibodies; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D018093:Gelatinases; D039121:Integrin alpha6beta1; D016023:Integrins; D007797:Laminin; D020778:Matrix Metalloproteinase 2; D008565:Membrane Proteins; D008666:Metalloendopeptidases; D009842:Oligopeptides; D010446:Peptide Fragments; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha; C102308:AG 10 peptide; C092699:GD-2 peptide