An automated and portable low-flow pulsatile perfusion system for organ preservation.
While machine preservation reduces the incidence of delayed graft function in renal transplant recipients, it is only used in 10% of kidney transplantations. The performance of our portable, low-flow-pulsatile organ perfusion system was examined in a canine kidney autotransplantation model. Grafts were stored for 72 h by simple cold preservation in University of Wisconsin (UW) solution, or by high or low-flow machine preservation After preservation, the grafts were autotransplanted and the animals were followed for 15 days. Graft function was better in machine-preserved kidneys. Tissue biochemistry indicated that machine preservation resulted in higher levels of adenine nucleotides and better histological integrity than the cold storage. While histology and biochemistry of machine-preserved groups were similar, electromicroscopy of high-flow grafts showed mild accumulation of intravenous debris and endothelial swelling. This study shows that a simplified machine perfusion technique is effective for organ preservation.
Pittsburgh Transplantation Institute, University of Pittsburgh, PA 15260, USA.
M J
MJ
Yland
S
S
Todo
Y
Y
Zhu
T
T
Suzuki
N
N
Hamada
S
S
Zhang
V M
VM
Subbotin
I
I
Takeyoshi
C
C
Doria
S
S
Hikida
A
A
Nemoto
R
R
Venkataramanan
P
P
Tata
J F
JF
Bernardo
D000818:Animals; D004285:Dogs; D005260:Female; D007668:Kidney; D008297:Male; D009926:Organ Preservation / Q000295:instrumentation*; D010477:Perfusion / Q000295:instrumentation*