The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin.
The cerebrospinal fluid (CSF) of patients with multiple sclerosis or Guillain-Barré syndrome contains a factor that inhibits excitation of nerve and muscle cells like local anesthetics. CSF samples containing the endogenous local anesthetic-like factor (ELLF) were analyzed by gel filtration chromatography and ultraviolet (UV) absorption at 210 nm. The active component was in a single peak corresponding to a molecular weight of 600-800 Da. This peak was decreased and the Na+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na+ channel blocking activity reappeared. The ELLF and its neutralization may be of relevance for the clinical fluctuations known with these diseases.
Abteilung für Allgemeine Physiologie, Universität Ulm, Germany.
P
P
Aulkemeyer
H
H
Brinkmeier
K H
KH
Wollinsky
R
R
Rüdel
D002555:Cerebrospinal Fluid; D002850:Chromatography, Gel; D003711:Demyelinating Diseases / Q000134:cerebrospinal fluid; D006801:Humans; D011129:Polyradiculoneuropathy / Q000134:cerebrospinal fluid; D011485:Protein Binding / Q000502:physiology; D012709:Serum Albumin / Q000502:physiology*; D015222:Sodium Channels / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D012709:Serum Albumin; D015222:Sodium Channels