Characterization of monoclonal antibodies against (1R,2R)-cyclohexanediamine platinum(II)-DNA adduct.
Two monoclonal antibodies raised against Pt(II)(1R,2R-cyclohexanediamine)-DNA were prepared, and the specificity of the monoclonal antibodies against Pt(II)(cyclohexanediamine)-DNA derivatives was determined by competitive enzyme-linked immunosorbent assay. The binding affinity of the monoclonal antibodies is apparently influenced by the cyclohexanediamine moiety of Pt(II)(cyclohexanediamine)-DNA adducts, but the monoclonal antibodies can not bind to the low molecular analogue, Pt(II)(1R,2R-cyclohexanediamine)-d(GpG), which is the intrastrand binding model compound of Pt(II)(1R,2R-cyclohexanediamine)-DNA. Therefore, the monoclonal antibodies recognize the macromolecular parts, including DNA duplex, in addition to the cyclohexane moieties of the platinum complexes on Pt(II)(cyclohexanediamine)-DNA adducts.
Department of Food Science and Technology, Faculty of Engineering, Fukuyama University, Japan.
J
J
Hirose
K
K
Inoue
E
E
Morimoto
H
H
Iwamoto
Y
Y
Yamaguti
M
M
Kitase
K
K
Inagaki
K
K
Hiromi
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000276:immunology; D002945:Cisplatin / Q000737:chemistry* / Q000276:immunology; D018736:DNA Adducts / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D018736:DNA Adducts; D002945:Cisplatin