Evidence for a single pool of myo-inositol in hormone-responsive WRK-1 cells.
Previous reports have suggested the existence of at least two pools of cellular myo-inositol (Ins); it has been further hypothesized that only one of these pools is utilized during hormone-activated, cyclic phosphatidylinositol (PtdIns) resynthesis. In an effort to investigate this possibility, we have undertaken kinetic studies of Ins metabolism in WRK-1 cells. Our results indicate that a single pool of Ins is involved in both basal and activated PtdIns synthesis. Ins generated by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) mixes with the existing pool of free Ins and is not used exclusively for resynthesis of PtdIns.
Department of Physiology and Biophysics, New York University Medical Center, 10010, USA.
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D000818:Animals; D007294:Inositol / Q000032:analysis*; D007295:Inositol Phosphates / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000494:pharmacology
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D014667:Vasopressins; D007294:Inositol; D008094:Lithium