Immunogenicity of a peptide from a major neutralising determinant of the feline immunodeficiency virus surface glycoprotein.
The third variable region (V3) of the feline immunodeficiency virus (FIV) surface glycoprotein is predicted to have similar physical properties to that of HIV and has been shown to contain immunodominant and neutralizing epitopes. Immunological characteristics of this region were investigated further using a peptide corresponding to the middle of the putative FIV V3 loop. The peptide was recognized in ELISA by sera from the majority of naturally FIV-infected cats, and absorbed a significant fraction of the virus neutralizing activity from a pool of sera of cats naturally infected with FIV, confirming the immunogenic nature of this region. A sheep immunized with an octameric form of the peptide (multiple antigenic peptide; MAP) in Freund's complete adjuvant generated neutralizing antibody to a higher titre than infected cats. However, immunization of cats with the same MAP in an acceptable adjuvant formulation (Quil A) induced antibody and cytotoxic T-cell responses to the immunizing peptides but only minimal neutralizing activity. These responses did not significantly alter the kinetics of infection or the proviral load after challenge with a homologous strain of FIV, compared with naive controls. While the potential efficacy of peptide vaccines to lentiviruses remains to be determined, this study shows that the immune response evoked may be highly dependent on the delivery and adjuvant regime used.
MRC Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, UK.
M A
MA
Rigby
N
N
Mackay
G
G
Reid
R
R
Osborne
J C
JC
Neil
O
O
Jarrett
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002415:Cats; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification; D016056:Immunodominant Epitopes / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology*; D011991:Receptors, Virus / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019562:Viral Load
D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D010455:Peptides; D011991:Receptors, Virus; C074548:feline leukemia virus receptor