Physical map of a YAC contig containing the region of the human gene (HYRC) complementing hyper-radiosensitivity of the scid mouse mutation.
We previously mapped the putative human HYRC (the hyper-radiosensitivity of the scid mutation, complementing gene) to human chromosome 8q11.1 by fluorescence in situ hybridization (FISH) using Alu-based PCR products from a mouse-human scid radiation cell hybrid (RD15/5) as probes. From a cosmid library constructed from RD15/5, 57 cosmid clones containing human DNA inserts were isolated. 18 of which were mapped to 8q11. Based on the sequences of plasmid subclones of the 18 cosmids, five novel sequence-tagged-sites (STSs) were made. By a screening of the CEPH-YAC library with these STSs, five yeast artificial chromosome (YAC) clones were isolated. All these YAC clones were confirmed not to be chimeric by FISH, but two of them showed deleted human insert DNAs. Using the other 3 non-deleted YACs, we constructed a physical map covering the HYRC region. We confirmed that the recently isolated gene (the DNA-PKcs gene) which is a strong candidate for HYRC is located within the present contig and spans less than 200 kb. This map will be useful for the analysis of the genomic structure of the DNA-PKcs gene and for isolation of other complementing genes in the HYRC region.
Watanabe
Y
Y
Department of Human Genetics, Nagasaki University School of Medicine, Japan.
Matsumoto
N
N
Ohta
T
T
Tsujita
T
T
Niikawa
N
N
D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002898:Chromosomes, Human, Pair 8; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D016513:Mice, SCID / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance / Q000235:genetics*