High affinity of a fucosylated chondroitin sulfate for plasma low density lipoprotein.
Factors that influence the binding of sulfated polysaccharides to plasma low density lipoprotein (LDL) were investigated. Among the naturally occurring polysaccharides tested, a fucosylated chondroitin sulfate from an echinoderm exhibited the strongest interaction with LDL. Defucosylation and desulfation totally abolished the interaction with LDL while reduction of carboxyl groups had little effect. These data indicate that the sulfated fucose branches are essential for binding of fucosylated chondroitin sulfate to LDL. In addition, there was a positive correlation between the binding to LDL and increasing length of the sulfated polysaccharide chains. The possibility of a practical use of this fucosylated chondroitin sulfate for the binding of LDL is discussed.
Departamento de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Brazil.
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Tovar
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Mourão
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism*; D012616:Sea Cucumbers; D012617:Sea Urchins; D013431:Sulfates / Q000378:metabolism
D008077:Lipoproteins, LDL; D013431:Sulfates; D005643:Fucose; D002809:Chondroitin Sulfates