Differential expression of Na,K-ATPase isoforms in oligodendrocytes and astrocytes.
Using Western blot and immunocytochemistry to detect protein, and in situ hybridization and Northern blot to detect RNA, we studied the expression of Na,K-ATPase subunit isoforms in cultured astrocytes and oligodendrocytes in vitro, and in glial cells in the brain. We found that astrocytes in culture express alpha 1 and alpha 2 isoforms of the catalytic subunit and both beta 1 and beta 2 isoforms of the regulatory subunit. Oligodendrocytes in culture express only alpha 1, but in vivo high levels of alpha 2 are seen in oligodendrocytes of white matter tracts. These results suggest that expression of the alpha 2 isoform in mature oligodendrocytes may be regulated by axonal contact and related to myelination.
Department of Neurology, University of Pittsburgh, Pa., USA.
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D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology*; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000201:enzymology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D009836:Oligodendroglia / Q000166:cytology / Q000201:enzymology*; D015347:RNA Probes; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000096:biosynthesis*
D007527:Isoenzymes; D015347:RNA Probes; D000254:Sodium-Potassium-Exchanging ATPase