Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci.
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.
Léon-Kloosterziel
K M
KM
Department of Genetics, Wageningen Agricultural University, The Netherlands.
Gil
M A
MA
Ruijs
G J
GJ
Jacobsen
S E
SE
Olszewski
N E
NE
Schwartz
S H
SH
Zeevaart
J A
JA
Koornneef
M
M
D000040:Abscisic Acid / Q000096:biosynthesis / Q000235:genetics*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D017343:Genes, Plant; D005808:Genes, Recessive; D018525:Germination; D008027:Light; D008540:Meiosis; D009154:Mutation; D011092:Polyethylene Glycols / Q000494:pharmacology; D012641:Selection, Genetic; D012965:Sodium Chloride / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D014230:Triazoles; D014867:Water; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C053370:paclobutrazol; D000040:Abscisic Acid