Effect of divalent cations on the contractile response of rat aorta to depolarization before and after nifedipine treatment.
The influence of the divalent cations, Ca2+, Mg2+ and Ba2+, on the contractile response of the rat aorta to KCl and on the recovery of this response after nifedipine treatment was analyzed. KCl (80 mmol/l) promoted a two-phase (phasic and tonic) contractile response in Krebs solution but, as expected, no contractile response in Ca(2+)-free medium. In Mg(2+)-free medium, the phasic response to KCl was unaffected but the tonic one decreased slowly, suggesting that a long incubation time in the absence of Mg2+ (65 min) promotes a loss of or a change in the intracellular distribution of this ion that modifies Ca2+ entry through L channels or Ca2+ handling. Ba2+ (1.8 mmol/l) contracted the rat aorta in the absence or presence of Ca2+ but, when Ca2+ was not present, Ba2+ modified the contractile process so that a new addition of KCl did not reproduce the contractile response. After nifedipine treatment, no phasic response to KCl was observed and only a slow response appeared that increased as long as the KCl was present (45 min). After washing, KCl induced a phasic response similar to the standard response, but a smaller tonic one. Divalent cations accelerated the recovery of the channels involved in the tonic contraction. However, the recovery of the Ca2+ channels related to the phasic contraction was independent of divalent cations.
Noguera
M A
MA
Departamento de Farmacología, Facultat de Farmacia, Universitat de Valencia, Espana.
Chulia
S
S
Elorriaga
M
M
Ivorra
M D
MD
D'Ocon
P
P
D000704:Analysis of Variance; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002413:Cations, Divalent / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D002413:Cations, Divalent; D011189:Potassium Chloride; D009543:Nifedipine