Reactivity of monoclonal antibodies of the B cell panel on PBM from BLV-infected and lymphocytotic cows.
The monoclonal antibodies included in the B cell panel of the Third Workshop on Ruminant Leukocyte Antigens were tested by flow cytometry for their reactivity with peripheral blood mononuclear cells (PBM) from normal, BLV (bovine leukemia virus)-infected but non-lymphocytotic and lymphocytotic cows. Three MoAbs probably detected pan-B cell antigens. They detected an increase in the B/T cell ratio in peripheral blood from lymphocytotic cattle. However, no changes were observed in the surface phenotypes of B cells from infected animals with MoAbs of the workshop panel.
Istituto di Microbiologia e Immunologia, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Italy.
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Poli
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Turin
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Rocchi
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Ponti
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005260:Female; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008218:Lymphocytosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor