Flow cytometric analysis of the reactivity of workshop monoclonal antibodies on sheep lymph node cells, after infection with Salmonella abortusovis.
Of the 302 monoclonal antibodies included in the Third Workshop on Ruminant Leukocyte Antigens, 167 have been tested for their reactivity on uninfected and Salmonella-infected sheep lymph node leukocytes using FACS analysis. Only 47 of them showed specificities which could be related to those of some control monoclonal antibodies, such as the anti-CD5, anti-Ig light chain and anti-MHC Class II monoclonal antibodies.
Bernard
S
S
INRA, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
Olivier
M
M
Bernard
F
F
Berthon
P
P
Doucet
F
F
Lantier
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000145:classification / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000145:classification* / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology; D012475:Salmonella / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012756:Sheep
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal