Structural study and serological characterisation of the O-specific polysaccharide of Hafnia alvei PCM 1185, another Hafnia O-antigen that contains 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose.
The O-specific polysaccharide of H. alvei strain PCM 1185 contains D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose (Qui3NAcyl) in the ratios 2:1:1:1 as well as O-acetyl groups. On the basis of sugar and methylation analyses of the polysaccharide before and after chemical modifications (O-deacetylation, carboxyl reduction, Smith degradation), as well as 1H and 13C NMR spectroscopy, including 1D sequential, selective spin-decoupling, 2D homonuclear and 13C,1H heteronuclear correlation spectroscopy (COSY), and 2D rotating-frame NOE spectroscopy, it was found that the polysaccharide has a pentasaccharide repeating unit with the following structure: [formula: see text] with O-acetyl groups present in nonstoichiometric amounts, mainly at position 2 of GlcA and position 6 of GlcNAc or lateral Glc. Serological study showed that H. alvei strain PCM 1185 can be placed in a new serotype D and that an O-acetyl group can be a part of its epitope.
L.Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
E
E
Katzenellenbogen
J
J
Kübler
A
A
Gamian
E
E
Romanowska
A S
AS
Shashkov
N A
NA
Kocharova
Y A
YA
Knirel
N K
NK
Kochetkov
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011233:Precipitin Tests; D012091:Repetitive Sequences, Nucleic Acid
C103125:3,6-dideoxy-3-(3-hydroxybutyramido)glucose; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D005947:Glucose