Transient appearance of GTP cyclohydrolase I--positive non-monoaminergic neurons in the ventral lateral geniculate nucleus of postnatal mice.
The transient appearance of GTP cyclohydrolase I (GCH)-immunoreactive (ir) cells in the ventral lateral geniculate nuclear region of mice was detected by use of an avidin-biotin peroxidase complex method with an antibody specific for an oligopeptide of rat GCH (residues from 12 to 23, GFPERELPRPGA). In this brain region, we found for the first time novel GCH-ir cells already at postnatal day 1 (P1). The numbers reached maximum at P14 and decreased until P29, and they had mostly disappeared by P56. These cells were tyrosine hydroxylase negative and aromatic L-amino acid decarboxylase negative, indicating a lack of dopamine or serotonin production, and thus do not belong to the monoaminergic neuron system.
Department of Anatomy, School of Medicine, Fujita Health University, Aichi, Japan.
I
I
Nagatsu
T
T
Takeuchi
M
M
Sakai
R
R
Arai
N
N
Karasawa
T
T
Nagatsu
D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology; D002540:Cerebral Cortex / Q000166:cytology; D006136:GTP Cyclohydrolase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005829:Geniculate Bodies / Q000166:cytology*; D006624:Hippocampus / Q000166:cytology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D018377:Neurotransmitter Agents / Q000032:analysis / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D012701:Serotonin / Q000032:analysis / Q000276:immunology; D013477:Superior Colliculi / Q000166:cytology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000276:immunology / Q000378:metabolism
D018377:Neurotransmitter Agents; D012701:Serotonin; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D009252:NADPH Dehydrogenase; D006136:GTP Cyclohydrolase; D001142:Aromatic-L-Amino-Acid Decarboxylases