Development and validation of an LC-MS-MS method for the determination of terfenadine in human plasma.
A sensitive LC-MS-MS method capable of quantifying terfenadine at levels down to 100 pg ml-1 in human plasma is reported. The method was validated over a linear range from 0.1 to 5.0 ng ml-1 using a liquid-liquid extraction with a deuterium-labelled internal standard. The between-run precision and accuracy of the calibration standards were 2.6-6.0% RSD and -2.0 to +2.2% relative error (RE). The between-run and within-run precision and accuracy of quality control samples (0.3, 1.5 and 3.5 ng ml-1) were 1.0-5.9% RSD and +1.7 to +6.3% RE. This method was applied to the analysis of human plasma samples.
Xu
A
A
Harris Laboratories, Lincoln, NE 68501, USA.
Linderholm
K
K
Peng
L
L
Hulse
J
J
D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D016593:Terfenadine / Q000097:blood*
D006634:Histamine H1 Antagonists; D016593:Terfenadine