Detection of mycoplasma DNA in veterinary live virus vaccines by the polymerase chain reaction.
We examined bovine, porcine, canine, feline, avian and mink veterinary live vaccines for the presence of mycoplasma DNA by the polymerase chain reaction including nested-pair primers. Mycoplasma DNA was detected in 22 of the 61 commercial veterinary vaccines (36.1%), although these vaccines did not contain any viable mycoplasma cells.
National Veterinary Assay Laboratory, Tokyo, Japan.
A
A
Kojima
T
T
Takahashi
M
M
Kijima
Y
Y
Ogikubo
Y
Y
Tamura
R
R
Harasawa
D000818:Animals; D001717:Birds; D002415:Cats; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics*; D004285:Dogs; D004340:Drug Contamination; D008907:Mink; D009174:Mycoplasma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013552:Swine; D014613:Vaccines, Attenuated / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*
D004269:DNA, Bacterial; D014613:Vaccines, Attenuated; D014765:Viral Vaccines