Ferritin mRNAs in Schistosoma mansoni do not have iron-responsive elements for post-transcriptional regulation.
Schistosoma mansoni possesses two isoforms of ferritin, soma and yolk ferritin. The soma ferritin occurs at a low level in most cells of both genders, whereas the yolk ferritin is a female-specific gene product that is expressed at high level in the vitellarium. In higher animals, ferritin mRNA is regulated by iron via the interaction of cytoplasmic binding proteins (IRPs) with a specific sequence element in the 5' untranslated region (UTR) referred to as the iron-responsive element (IRE). Sequence studies of the 5' UTRs, gel retardation assays, and hybridization experiments show that neither ferritin mRNAs of S. mansoni is regulated by an IRE/IRP mechanism. It is suggested that ferritins in schistosomes are controlled only at the transcriptional level.
Institut für Genetik and Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität, Düsseldorf, Germany.
P
P
Schüssler
E
E
Pötters
R
R
Winnen
A
A
Michel
W
W
Bottke
W
W
Kunz
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002457:Cell Extracts / Q000737:chemistry; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005293:Ferritins / Q000145:classification / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012550:Schistosoma mansoni / Q000737:chemistry / Q000378:metabolism*; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics
D002457:Cell Extracts; D015342:DNA Probes; D005293:Ferritins; D007501:Iron