CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis.
Overexpression of B7 and intracellular adhesion molecule-1 (ICAM-1) on syngeneic cells triggered MHC-unrestricted lysis by MHC-restricted CD8+ CTL without TCR/CD3 engagement. Both CD28/B7 and LFA-1/ICAM-1 interactions were required for MHC-nonrestricted cytotoxicity. Cytotoxicity was measured in 4-h and 16- to 18-h cytotoxicity assays. B7-ICAM-1 overexpression triggered perforin- and Fas ligand-mediated lysis, while TNF-alpha was not elicited in MHC-unrestricted cytotoxicity. These results suggest that target cells may elicit MHC-unrestricted lysis from CTL through up-regulation of membrane adherence and costimulatory receptors.
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33136, USA.
M
M
Nishio
J
J
Spielman
R K
RK
Lee
D L
DL
Nelson
E R
ER
Podack
D000818:Animals; D018122:B7-1 Antigen / Q000494:pharmacology*; D018106:CD28 Antigens / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004198:Disease Susceptibility; D053222:Fas Ligand Protein; D007114:Immunization / Q000379:methods; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology*; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000494:pharmacology; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects*
D018122:B7-1 Antigen; D018106:CD28 Antigens; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin; D018799:Intercellular Adhesion Molecule-1