Quantification of retinoyl-beta-glucuronides in rat urine by reversed-phase high-performance liquid chromatography with ultraviolet detection.
A method is presented for the quantitation of the glucuronide conjugates of 4-oxo-all-trans-, 4-oxo-13-cis-, 13-cis-, 9-cis- and all-trans-retinoic acids in rat urine utilizing solid-phase extraction and gradient reversed-phase HPLC. The range of the R.S.D. (relative standard deviation) for both the inter- and intra-assay precision was 1.45-11.60%. The recovery of all retinoyl-beta-glucuronides from rat urine ranged between 89 and 99%. The limit of detection was 0.01 microgram/ml using 5 ml of rat urine. This method was applied to quantitate the amount of retinoyl-beta-glucuronides produced in urine after the single and multiple oral administration of 13-cis-, 9-cis- and all-trans-retinoic acids to rats.
Li
S
S
Department of Drug Metabolism and Pharmacokinetics, Hoffmann-LaRoche, Inc., Nutley, NJ 07110, USA.
Barua
A B
AB
Huselton
C A
CA
D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004355:Drug Stability; D005965:Glucuronates / Q000737:chemistry / Q000652:urine*; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature; D014212:Tretinoin / Q000008:administration & dosage / Q000737:chemistry / Q000652:urine*
D000970:Antineoplastic Agents; D005965:Glucuronates; D014212:Tretinoin