Detection of intracellular signal transduction molecules in PBMC from rhesus macaques and sooty mangabeys.
One of the manifestations of human HIV-1 and nonhuman primate SIV infection that lead to disease is reasoned to be secondary to generalized T-cell dysfunction. The molecular mechanisms associated with the T-cell dysfunction remain to be elucidated. To address this issue, we sought to utilize the nonhuman primate model to study intracellular signaling events in cells from disease-susceptible rhesus macaques and disease-resistant sooty mangabeys. Because relatively little is known about these events in nonhuman primates, our laboratory defined optimal conditions, reagents, and assays for the study of signal transduction events in cells from nonhuman primates. The protein phosphorylation patterns in the two monkeys exhibited quantitative, qualitative, and kinetic differences. Antibodies to Stat6 detected a unique band in macaque cell lysates. This band is markedly decreased human cell lysates and never seen in mangabey cell lysates. Detection of various other intracellular signaling proteins is also described.
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
G T
GT
Brice
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Villinger
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Mayne
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JB
Sundstrom
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Ansari
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016665:Cercocebus atys; D004268:DNA-Binding Proteins / Q000032:analysis; D004198:Disease Susceptibility; D051380:GRB2 Adaptor Protein; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007527:Isoenzymes / Q000032:analysis; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000502:physiology; D008253:Macaca mulatta; D051966:Phospholipase C gamma; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D050794:STAT1 Transcription Factor; D050795:STAT2 Transcription Factor; D015398:Signal Transduction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D015534:Trans-Activators / Q000032:analysis; D010738:Type C Phospholipases / Q000032:analysis; D018631:ras Proteins / Q000032:analysis
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D007527:Isoenzymes; D010750:Phosphoproteins; D011506:Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D050795:STAT2 Transcription Factor; D015534:Trans-Activators; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D018631:ras Proteins