Molecular cloning and characterization of murine interleukin-11.
Human interleukin-11 (IL-11) has been shown to have pleiotropic action on hematopoietic, hepatic, stromal, epithelial, neural, and osteoclast cells. In the present work, the murine IL-11 cDNA has been isolated from a fetal thymic cell line, and its structure and function compared with human IL-11. The murine protein was demonstrated to have identical actions on the proliferation of a murine plasmacytoma cell line, murine primitive bone marrow progenitor cells, and megakaryocyte precursors. The murine IL-11 protein was synthesized as a soluble thioredoxin-IL-11 fusion in Escherichia coli and the expression of murine IL-11 was examined by pulse-chase radiolabeling in COS cells. The chromosomal location of the murine IL-11 gene was assigned to the proximal arm of chromosome 7.
Genetics Institute, Inc., Cambridge, MA 02140, USA.
J C
JC
Morris
S
S
Neben
F
F
Bennett
H
H
Finnerty
A
A
Long
D R
DR
Beier
S
S
Kovacic
J M
JM
McCoy
E
E
DiBlasio-Smith
E R
ER
La Vallie
A
A
Caruso
J
J
Calvetti
G
G
Morris
N
N
Weich
S R
SR
Paul
P S
PS
Crosier
K J
KJ
Turner
C R
CR
Wood
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells / Q000737:chemistry; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005333:Fetus / Q000166:cytology; D006801:Humans; D017370:Interleukin-11 / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015394:Molecular Structure; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology; D014162:Transfection
D018076:DNA, Complementary; D017370:Interleukin-11; D012333:RNA, Messenger