Crystallization and X-ray analysis of the reaction center from the thermophilic green bacterium Chloroflexus aurantiacus.
The photochemical reaction center of Chloroflexus (Cf.) aurantiacus, a membrane bound pigment-protein complex, has been crystallized in the presence of monodisperse polyoxyethylene detergents. The crystals possessed a pronounced polymorphism. Three different crystal forms belonging to triclinic, monoclinic and orthorhombic space groups have been characterized by X-ray analysis. The triclinic crystal form, with unit cell dimensions of a = 88 A, b = 115 A and c = 151 A, diffracts up to 3.2 A in two directions and to 4.0 A in the third direction.
Feick
R
R
Max-Planck-Institut für Biochemie, Martinsried, Germany. feick@biochem.mpg.de
Ertlmaier
A
A
Ermler
U
U
D019414:Chlorobi / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification
D045322:Photosynthetic Reaction Center Complex Proteins