Identification of cis-acting elements involved in an alternative splicing of the amyloid precursor protein (APP) gene.
Our previous study demonstrated that a cis-acting element for neuron-specific splicing of human amyloid precursor protein (APP) gene existed in an intron region flanking to exon 8 (Yamada et al., 1993). In this study, we extended the analysis by deletion and mutagenesis experiments and identified two distinct cis-acting elements which modulate the splicing of exon 8 of the APP gene in a sequence-dependent manner. Both elements are located at the just upstream region of the so-called branchpoint sequence, the first (ATGTTT) at -42 to -47 nt and the second (TTT) at -36 to -38 nt upstream from exon 8. Our study suggested that the first one is a positive element for the splicing of exon 8 and the second a negative one for the splicing.
Shibata
A
A
Human Genome Center, University of Tokyo, Japan. asamis@hgc.ims.u-tokyo.ac.jp
Hattori
M
M
Suda
H
H
Sakaki
Y
Y
D017398:Alternative Splicing; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D005091:Exons / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry / Q000235:genetics; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D016564:Amyloid beta-Protein Precursor; D012313:RNA