Clustered localization of oligomeric Nef protein of human immunodeficiency virus type 1 on the cell surface.
We studied human immunodeficiency virus type 1 (HIV-1) Nef protein biochemically and histologically. HIV-1 Nef, derived from baculosystem and from cells infected with HIV-1, formed homomeric monomers, dimers, trimers, and further polymers. These oligomers were non-covalently associated. In cells infected with HIV-1, Nef molecules were clustered at the cell surface as well as cytoplasm. Our previous results have indicated that the Nef on the surface of cells infected with HIV-1 is cytotoxic against uninfected CD4+ T cells. Thus, it is very likely that the HIV-1-mediated cytotoxic reaction is due, at least in part, to the clustered localization of oligomeric Nef on the cell surface.
Fujii
Y
Y
Institute for Laboratory Animal Research, Nagoya University School of Medicine, Japan.
Otake
K
K
Fujita
Y
Y
Yamamoto
N
N
Nagai
Y
Y
Tashiro
M
M
Adachi
A
A
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D019084:Fluorescent Antibody Technique, Indirect; D015693:Gene Products, nef / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D018411:Spodoptera; D014162:Transfection; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus