Simultaneous determination of furprofen and rufloxacin in human plasma by high-performance liquid chromatography.
A simple and reproducible method for the simultaneous determination of the nonsteroidal anti-inflammatory agent, furprofen, and the quinolone antimicrobial agent, rufloxacin, in human plasma is described. It involves a two-step liquid-liquid extraction and a separation using an LC-SAX column with ultraviolet detection at 280 nm. Fenbufen is used as the internal standard. Within-day and between-day coefficients of variation are less than 6%. The lower limits of detection are 0.05 and 0.03 micrograms/mL for furprofen and rufloxacin, respectively. The method is suitable for pharmacological, toxicological, and pharmacokinetic studies of furprofen and rufloxacin.
Dipartimento di Chimica, Università dell'Aquila, Italy.
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Carlucci
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Mazzeo
D000890:Anti-Infective Agents / Q000097:blood*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones; D006801:Humans; D010666:Phenylpropionates / Q000097:blood*; D015363:Quinolones / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D024841:Fluoroquinolones; D010666:Phenylpropionates; D015363:Quinolones; C055623:furprofen; C060328:rufloxacin