c-fos CRE-binding activity of CREB/ATF family in the SCN is regulated by light but not a circadian clock.
The DNA-binding activity of cAMP-responsive element binding protein (CREB) in the suprachiasmatic nucleus (SCN) was examined with Ca or cAMP-responsive element (Ca/CRE) in upstream sequence of c-fos gene using electro-mobility shift assay (EMSA). By using supershift assay, Ca/CRE-binding activity in the SCN was shown to contain not only CREB but also activating transcription factor-1 (ATF-1). Furthermore, photic-induction of CREB binding activity to Ca/CRE in the SCN occurred at all circadian times tested, indicating that CREB DNA-binding in the SCN was not gated by the endogenous clock. These results implicated the role of CREB/ATF family in photic neuronal signaling in the SCN.
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
K
K
Kako
H
H
Wakamatsu
N
N
Ishida
D051697:Activating Transcription Factor 1; D000818:Animals; D001483:Base Sequence; D002940:Circadian Rhythm; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins; D008297:Male; D008969:Molecular Sequence Data; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D051697:Activating Transcription Factor 1; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D016760:Proto-Oncogene Proteins c-fos; D014157:Transcription Factors