Putative serine/threonine protein kinase expressed in complement-resistant forms of Entamoeba histolytica.
Entamoeba histolytica is susceptible to complement attack in its lumen-dwelling state and develops complement resistance during pathogenic tissue invasion. As experimental evidence suggests that this change in phenotype is accompanied by a change in gene expression, we constructed a subtractive cDNA library to identify genes involved. Poly(A) + RNA from complement-sensitive trophozoites was subtracted from single stranded cDNA derived from complement-resistant ones. Transcripts enriched in the library were found to code for a putative polypeptide comprising all sequence elements characteristic for serine/threonine protein kinases. The gene contains an intron of 46 nucleotides and two polyadenylation sites. Northern-blot analyses confirmed that the gene is expressed in both tissue-derived and laboratory-grown forms of complement-resistant E. histolytica.
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
B
B
Urban
C
C
Blasig
B
B
Förster
C
C
Hamelmann
R D
RD
Horstmann
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003165:Complement System Proteins / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D016054:DNA, Protozoan; D003165:Complement System Proteins; D017346:Protein Serine-Threonine Kinases