Phenylhydrazine causes sulfhydryl oxidation and protein aggregation in hemoglobin-free human erythrocyte membranes.
Phenylhydrazine induces a remarkable decrease in total free sulfhydryls as well as spectrin and most other polypeptides in hemoglobin-free human erythrocyte ghosts. A parallel increase in coomassie blue stainable material that was retained on the gel tops was also observed. EDTA but not DM protected the membrane from phenylhydrazine-induced alterations, while antioxidants, catalase and SOD were ineffective. The aggregated material isolated from phenylhydrazine-treated membranes could be partially dissociated in presence of beta-mercaptoethanol to yield most of the membrane polypeptides.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India.
A N
AN
Hashmi
M
M
Saleemuddin
D000975:Antioxidants / Q000494:pharmacology; D001798:Blood Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism / Q000494:pharmacology; D003676:Deferoxamine / Q000378:metabolism / Q000494:pharmacology; D004220:Disulfides / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D010659:Phenylhydrazines / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism / Q000494:pharmacology
D000975:Antioxidants; D001798:Blood Proteins; D004220:Disulfides; D006454:Hemoglobins; D010659:Phenylhydrazines; D013438:Sulfhydryl Compounds; C030299:phenylhydrazine; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D003676:Deferoxamine