Effect of the crystallizing agent 2-methyl-2,4-pentanediol on the structure of adenine tract DNA in solution.
The hydroxyl radical cleavage pattern of bent DNA containing phased adenine tracts has a sinusoidal pattern. Changes in the cleavage pattern of the sequence A5N5 in the presence of 2-methyl-2,4-pentanediol (MPD) demonstrate that MPD has an effect on the structure of a bent A-tract region of DNA. The effect of MPD on DNA structure appears to be limited to bent A-tract regions, as we find no significant changes in the cleavage pattern of flanking regions, mixed-sequence DNA, or the unbent A-tract sequence T4A4N2.
Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA.
R M
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Ganunis
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Guo
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Tullius
D003461:Crystallography; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D006018:Glycols / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011061:Poly A / Q000378:metabolism*
D006018:Glycols; D011061:Poly A; D017665:Hydroxyl Radical; D004247:DNA; C011480:hexylene glycol