An Aeromonas salmonicida gene required for the establishment of infection in rainbow trout (Oncorhynchus mykiss).
The asoB gene of Aeromonas salmonicida is located approximately 9 kb downstream of the structural gene (vapA) for the surface layer (A-layer). The nucleotide sequence of asoB was determined and found to encode a putative polytopic cytoplasmic membrane protein which exhibited homology to a number of bacterial transport proteins. Allele exchange mutagenesis of asoB resulted in a mutant (A449-D) which was avirulent when administered by bath immersion. However, when administered by intraperitoneal injection, A449-D is as lethal as wild type. Characterization of the phenotype of A449-D showed that there were pleiotropic effects on VapA secretion, haemolysis and outer membrane protein composition. Mobilization of cloned asoB on a broad-host-range plasmid into A449-D resulted in the complementation of VapA translocation, haemolytic activity and virulence.
Noonan
B
B
Department of Biochemistry and Microbiology, University of Victoria, B.C., Canada.
Trust
T J
TJ
D000333:Aeromonas / Q000235:genetics* / Q000472:pathogenicity; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D016366:Open Reading Frames / Q000235:genetics* / Q000502:physiology; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; C102191:AsoB protein, Aeromonas salmonicida; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins