Crystallization of NAD+ synthetase from Bacillus subtilis.
NAD(+)-synthetase is a ubiquitous enzyme catalyzing the last step in the biosynthesis of NAD+. Mutants of NAD+ synthetase with impaired cellular functions have been isolated, indicating a key role for this enzyme in cellular metabolism. Crystals of the enzyme from Bacillus subtilis suitable for x-ray crystallographic investigation have been grown from polyethylene glycol solutions. Investigation on the structural organization of NAD+ synthetase, an enzyme fundamental for NAD+ biosynthesis, and belonging to the recently characterized amidotransferase enzymatic family, will provide more insight into the catalytic mechanism of deamido-NAD+-->NAD+ conversion, a biosynthetic process that is a potential target for the development of antibiotic compounds against Bacillus sp. and related bacteria.
Rizzi
M
M
Department of Genetics and Microbiology, A. Buzzati Traverso, University of Pavia, Italy.
Nessi
C
C
Bolognesi
M
M
Coda
A
A
Galizzi
A
A
D019732:Amide Synthases; D001412:Bacillus subtilis / Q000201:enzymology*; D018360:Crystallography, X-Ray; D008025:Ligases / Q000737:chemistry*
D008025:Ligases; D019732:Amide Synthases; C038479:NAD+ synthase