Aberrant RNA splicing of the protein C and protein S genes in healthy individuals.
RNA-based studies are an important tool for the identification and functional characterization of mutations underlying inherited disease. These studies could in principle be compromised by 'aberrant splicing' (the generation of alternatively spliced transcripts lacking any obvious function) during normal expression of the genes under investigation. Using a highly sensitive RT-PCR assay, we show here that aberrant splicing is a frequent occurrence during expression of the protein C (PROC) and protein S (PROS) genes. Aberrantly spliced transcripts were present in different cell types including liver, the main expressing tissue for both protein C and protein S. In an attempt to compare individual mRNA splicing patterns, PROC and PROS RNA from easily accessible cells of different healthy control individuals was studied. However, variation between different RT-PCR assays from the same individual precluded both the relative quantitation of the aberrant transcripts and the analysis of interindividual differences. Our findings are consistent with the notion that a low level of aberrantly spliced transcripts are routinely generated during PROC and PROS gene expression. The possibility that these transcripts may complicate the RT-PCR analysis of pathological transcripts must be taken into account when RNA-based strategies of disease analysis are considered.
Molecular Genetics Section, Thrombosis Research Institute, London, UK.
L P
LP
Berg
J M
JM
Soria
C J
CJ
Formstone
M
M
Morell
V V
VV
Kakkar
X
X
Estivill
N
N
Sala
D N
DN
Cooper
D004587:Electrophoresis, Agar Gel; D005091:Exons; D006801:Humans; D016133:Polymerase Chain Reaction; D011486:Protein C / Q000235:genetics*; D017293:Protein S / Q000235:genetics*; D012326:RNA Splicing; D014158:Transcription, Genetic
D011486:Protein C; D017293:Protein S