Macrofragment localization of the toxin A and toxin B genes of Clostridium difficile.
We report the physical mapping of the toxin A and B genes to the bacterial chromosome of Clostridium difficile ATCC 43594 by pulsed-field gel electrophoresis. Single and double digestions with restriction endonucleases NruI and SacII allowed localization of the toxin genes to a specific 577-kb fragment and estimation of genome size to be approximately 3.8 megabases. This effort represents the initial step in the construction of a physical map of the whole genome.
Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA. dano@lehigh.edu.
D A
DA
Norwood
J A
JA
Sands
D002874:Chromosome Mapping / Q000379:methods*; D016360:Clostridioides difficile / Q000235:genetics*; D013744:Tetanus Toxin / Q000235:genetics*
D013744:Tetanus Toxin