The structure of the gene for the second chain of the human interferon-gamma receptor.
The gene for the second chain of the human interferon-gamma receptor was analyzed from cosmid DNA clones. The gene spans over 33 kilobases of DNA and contains seven exons. The signal peptide is encoded by exons 1 and 2, the extracellular domain by exons 2, 3, 4, 5, and by part of 6. Exon 6 also encodes the whole transmembrane domain and part of the intracellular domain. Exon 7 encodes the remainder of the intracellular domain and contains the 3'-untranslated region. The sequences at the exon/intron boundaries are well conserved with respect to canonical acceptor/donor sites (AG/GT). The 5'-flanking region was sequenced and analyzed for transcription factor binding sites. No TATA or CAAT boxes in the promoter region were identified. Consistent with the lack of a TATA box, analysis of the mRNAs by primer extension showed multiple transcription start sites. Promoter activity of the 5'-flanking region was investigated with a luciferase reporter gene and the cytomegalovirus minimal promoter. Segments of the 5' region with promoter activity were identified.
Rhee
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Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635, USA.
Ebensperger
C
C
Dembic
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Z
Pestka
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S
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017471:Receptors, Interferon / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D000096743:Interferon gamma Receptor
D015703:Antigens, CD; D004274:DNA, Recombinant; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor