Development of locustatachykinin immunopositive neurons in the central complex of the beetle Tenebrio molitor.
Locustatachykinin-immunoreactive (LomTK-IR) interneurons were found to be associated with the central complex, a prominent neuropil region of the insect brain. The structures and development of this set of brain interneurons was studied from the embryo onward in the beetle Tenebrio molitor, showing individual neurons that persist from the late embryo to the adult stage. Their essential structural characteristics were already present in the late embryo, but distinct parts of their arborization patterns became newly formed throughout development. Using a combination of immunohistochemistry and single-cell injection, we demonstrated minute structural changes, allowing a characterization of structural plasticity of identifiable, persistent, neuropeptidergic neurons throughout ontogenesis. Furthermore, this study has provided new information about basic principles of central brain neuroanatomy and the development of a distinct midbrain region of the insect brain, the central complex. The development of its basic connections, the connections between the fan-shaped body and the protocerebral bridge, and the compartmentation of these neuropil regions were shown, using LomTK-IR neurons as marker structures. These basic features of the central complex-associated LomTK-immunopositive neurons were formed in the embryonic brain, whereas in metamorphosis, reorganization of these persistent interneurons was restricted to the formation of a precisely defined projection of their side branches.
Wegerhoff
R
R
Biochemisches Institut, University of Kiel, Germany. rwegerhoff@biochem.uni-kiel.de
Breidbach
O
O
Lobemeier
M
M
D000818:Animals; D004625:Embryo, Nonmammalian / Q000737:chemistry; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000032:analysis*; D007814:Larva / Q000737:chemistry / Q000254:growth & development; D009474:Neurons / Q000737:chemistry*; D011679:Pupa / Q000737:chemistry / Q000254:growth & development; D015320:Tachykinins / Q000032:analysis*; D013712:Tenebrio / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development
D019476:Insect Proteins; D015320:Tachykinins; C063413:locustatachykinin I protein, Locusta migratoria