Challenges of high throughput screening against cell surface receptors.
Excessive or inappropriate activation of cell surface receptors can mediate the development of disease. Receptors, therefore, are a focus for drug discovery activities. Empirical screening is important in the search for novel compounds acting as receptors. Technical developments and the application of molecular biology have facilitated access to receptors of interest and have provided efficient screening methods capable of very high throughput. Reliability in high throughput screening requires the use of appropriate methodology, good screen design and effective validation and quality control processes. Validation should aim to establish that the basic experimental design is sound. In developing software to handle high throughput screening data, a fundamental requirement is to provide performance monitoring and error trapping facilities. Additional requirements are automatic data capture from instruments, on-line data reduction and analysis and transfer of results to central databases. As data volumes increase through effective high throughput screening, conventional interrogation methods become less appropriate and are being augmented by newer computing techniques referred to as knowledge mapping or database mining. Targeting cell surface receptors has been very successful as an approach to drug discovery. If the challenges of high throughput empirical screening are addressed effectively, cell surface receptors will provide new opportunities for improved therapy in the coming years.
Major
J S
JS
ZENECA Pharmaceuticals, Macclesfield, Cheshire, UK.
D000818:Animals; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D019332:Endothelin-1 / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011786:Quality Control; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D017466:Receptors, Endothelin / Q000378:metabolism; D015203:Reproducibility of Results; D012588:Scintillation Counting
D019332:Endothelin-1; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin