Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation.
The product of the yeast SEC12 gene is the most upstream player as far as we know in the events of vesicle budding from the endoplasmic reticulum membrane. To understand how the Sec12p function could be regulated, I identified three extragenic suppressor mutations of the sec12 ts mutation. RST1DS is a dominant mutation and causes elevated expression of Sec12p. rst2 and rst3 are recessive and give pleiotropic phenotypes including slow growth at low temperatures and heterogeneous modification of Sec12p. Their possible roles are discussed.
Department of Biological Sciences, Graduate School of Science, University of Tokyo. nakano@uts2.s.u.-tokyo.ac.jp
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Nakano
D004721:Endoplasmic Reticulum / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D005799:Genes, Dominant; D005800:Genes, Fungal; D005808:Genes, Recessive; D005838:Genotype; D020662:Guanine Nucleotide Exchange Factors; D008562:Membrane Glycoproteins / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic
D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D008562:Membrane Glycoproteins; C071239:SEC12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins