P-selectin induction of tissue factor biosynthesis and expression.
P-selectin on activated platelets and stimulated endothelial cells mediates cell adhesion with monocytes and neutrophils. Since activated platelets induce tissue factor on monocytes, we examined the effect of P-selectin on this activity. Chinese hamster ovary cells expressing P-selectin stimulated tissue factor activity in purified monocytes whereas untransfected Chinese hamster ovary cells and CHO cells expressing E-selectin did not. Anti-P-selectin antibodies inhibited these effects. Incubation of CHO:P-selectin with monocytes stimulates tissue factor mRNA development and the expression of tissue factor antigen on the monocyte surface. These results indicate that P-selectin upregulates the expression of tissue factor on monocytes.
Furie
B
B
Center for Hemostasis and Thrombosis Research, Division of Hematology-Oncology, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Furie
B C
BC
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000502:physiology; D019007:P-Selectin / Q000502:physiology*; D013925:Thromboplastin / Q000096:biosynthesis*
D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D013925:Thromboplastin