Parathyroid visualization with cibacron blue/BB5-G1.
BB5-G1 is a murine monoclonal antibody with proven affinity for human parathyroid tissue. The authors conjugated BB5-G1 to cibacron blue, a water-soluble dye, in an attempt to enhance intraoperative parathyroid visualization. Athymic nude mice possessing intramuscularly implanted human parathyroid tissue were infused with the conjugate, then surgically explored. Intraoperative findings were video-captured, then analyzed by three independent observers and by computer, generating subjective and objective data quantifying parathyroid visualization. Parathyroid tissue implants were stained and were easily visualized. The implants in the test mice displayed higher computer-generated color intensity ratings (mean, 10.25 units) compared to control mice (mean, 6.10 units) (p<0.5). Histologic sections confirmed the presence of dye and antibody within parathyroid tissue. This represents the first study to use such a conjugate to selectively enhance parathyroid visualization.
Department of Otorhinolaryngology, The University of Oklahoma Health Sciences Center, Oklahoma City, USA.
R C
RC
King
S L
SL
Mills
J E
JE
Medina
D000818:Animals; D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D005240:Feasibility Studies; D006801:Humans; D007432:Intraoperative Period; D051379:Mice; D008819:Mice, Nude; D010280:Parathyroid Glands / Q000473:pathology* / Q000637:transplantation; D016105:Parathyroidectomy; D011500:Protein Synthesis Inhibitors; D013194:Staining and Labeling; D014183:Transplantation, Heterologous; D014227:Triazines
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D011500:Protein Synthesis Inhibitors; D014227:Triazines; C012842:Cibacron Blue F 3GA