Establishment of monoclonal antibodies against carbohydrate moiety of gastric mucins distributed in the different sites and layers of rat gastric mucosa.
Eight monoclonal antibodies (MAbs), designated RGM21 approximately RGM42, were generated against mucin purified from the rat gastric mucosa. By applying ELISA, all of these MAbs were proved to react not only with the purified mucin, but also with the oligosaccharide mixture obtained from the antigenic mucin by alkaline borohydride treatment. Treatment of the mucin-attached ELISA well with trypsin, sodium periodate or galactose oxidase prior to the addition of the MAb was applied to characterize these MAbs. Histochemical observation indicated that all these MAbs were able to stain the formalin fixed-paraffin embedded sections of the rat gastroduodenal mucosa. Although each of these MAbs reacted with distinct mucus-producing cells localized in particular regions of the gastroduodenal mucosa, their staining specificity could generally be classified into four groups. These MAbs might be useful for estimating the physiological and pathological changes of mucins in the gastric mucosa.
Department of Chemistry, Kitasato University School of Medicine, Japan.
K
K
Ishihara
M
M
Kurihara
Y
Y
Goso
H
H
Ota
T
T
Katsuyama
K
K
Hotta
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001894:Borohydrides / Q000378:metabolism / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005692:Galactose Oxidase / Q000378:metabolism / Q000494:pharmacology; D005753:Gastric Mucosa / Q000737:chemistry*; D007150:Immunohistochemistry; D009077:Mucins / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001894:Borohydrides; D009077:Mucins; D009844:Oligosaccharides; C025364:sodium borohydride; D005692:Galactose Oxidase; D014357:Trypsin