Molecular cloning and gene expression of chum salmon cystatin.
A salmon cystatin cDNA clone was isolated from a chum salmon cDNA library. The clone encoded a full-length extracellular-type cystatin and its signal peptide and included 5'- and 3'-untranslated regions. The deduced amino acid sequence showed a high degree of sequence similarity to mammalian cystatin C, chicken egg cystatin, and chum salmon pituitary cystatin. By Northern blot analysis, the salmon cystatin was found to show apparently non-tissue specific expression. Because platyfish EHS cells transfected with a cystatin expression vector produced a 13 kDa mature cystatin in the culture medium, the salmon cystatin was considered to act as an extracellular type of cystatin in the fish cells. These findings indicate that the salmon cystatin is a homolog of mammalian cystatin C.
Yamashita
M
M
National Research Institute of Fisheries Science, Yokohama. mic@nrifs.affrc.go.jp
Konagaya
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015891:Cystatins / Q000096:biosynthesis* / Q000737:chemistry*; D017931:DNA Primers; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D017685:Oncorhynchus keta; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D015891:Cystatins; D017931:DNA Primers; D021382:Protein Sorting Signals; D011994:Recombinant Proteins