Two forms of alpha-glucosidase from Mucor javanicus produced by culture with castanospermine.
When Mucor javanicus was cultured with castanospermine, two alpha-glucosidases (CS-I, II) were produced. The two enzymes were purified by two CM-cellulofine column chromatographies, and compared with the alpha-glucosidase (C) from a culture without castanospermine. The molecular weights of the three enzymes were calculated to be 97,000 (CS-I, II) and 94,000 (C) from SDS-PAGE. CS-II was similar in hydrolysis of alpha-1,4-linkage to C, but hydrolyzed alpha-1,2-, alpha-1,3-, and alpha-1,6-linkages at a lower rate than C. The Michaelis constant of CS-I for malto-oligosaccharides was several times as high as the constants of CS-II and C, but CS-I hydrolyzed nigerose at a faster rate than maltose.
Yamasaki
Y
Y
Research Institute for Bioresources, Okayama University, Kurashiki, Japan.
Konno
H
H
D002240:Carbohydrate Sequence; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D007212:Indolizines / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000187:drug effects* / Q000201:enzymology*; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000096:biosynthesis* / Q000378:metabolism; D054836:Indolizidines
D005656:Fungal Proteins; D007212:Indolizines; D007527:Isoenzymes; D000520:alpha-Glucosidases; C037806:castanospermine; D054836:Indolizidines