The use of cultured hepatocytes from goats and cattle to investigate xenobiotic oxidative metabolism.
The oxidative metabolism of aldicarb (ALD), a carbamate pesticide, and fenbendazole (FBZ), an anthelmintic, was studied using cultured hepatocytes obtained from 4 goats and a bullock and incubated with ALD (50 mumol/L) and FBZ (10 mumol/L). The parent compounds and the metabolites were measured by HPLC. Both compounds are metabolized at the sulphur atom via two sequential oxidations, first to the sulphoxide (aldicarb sulphoxide and oxfendazole, respectively) and then to the sulphone. Oxfendazole and fenbendazole sulphone from FBZ, and aldicarb sulphoxide from ALD were found in both species. Aldicarb sulphone was not produced by the hepatocyte preparations from the bullock. The good correlation obtained comparing the in vitro results of FBZ metabolism with published in vivo data obtained on FBZ kinetics in the same species confirmed the usefulness of in vitro models for predictive analysis of in vivo xenobiotic biotransformations.
Institute of Veterinary Pathology and Hygiene, Faculty of Veterinary Medicine, University of Padova, Agripolis, Legnaro, Italy.
C
C
Montesissa
P
P
Anfossi
G
G
Van't Klooster
M
M
Mengelers
D000448:Aldicarb / Q000378:metabolism*; D000818:Animals; D000871:Anthelmintics / Q000378:metabolism; D001711:Biotransformation; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005273:Fenbendazole / Q000378:metabolism*; D006041:Goats; D007306:Insecticides / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D015262:Xenobiotics / Q000378:metabolism*
D000871:Anthelmintics; D007306:Insecticides; D015262:Xenobiotics; D005273:Fenbendazole; D000448:Aldicarb