Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis.
We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerophosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 micrograms/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
N
N
Akimitsu
T
T
Mizushima
E
E
Suzuki
T
T
Miki
K
K
Sekimizu
D004926:Escherichia coli / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D016296:Mutagenesis; D010641:Phenotype; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D010957:Plasmids / Q000378:metabolism; D013696:Temperature; D017855:Transferases (Other Substituted Phosphate Groups) / Q000096:biosynthesis*
D010715:Phosphatidylglycerols; D007612:Kanamycin; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase