Isolation of protein factors from opossum (Didelphis albiventris) serum which protect against Bothrops jararaca venom.
The fractionation of Didelphis albiventris serum by DEAE-Sephadex A50 yields a fraction (DA2) which protects the opossum against Bothrops venom. One polypeptide (DA2-II) responsible for this protection was isolated from fraction DA2 by ion exchange chromatography and biochemically characterized. DA2-II is a 43,000 mol. wt glycoprotein with the following N-terminal sequence: LKAMDTTPPLKIKKEPVK. Pairwise comparison of the amino acid sequence with four anti-hemorrhagic factors isolated from other opossum species indicated that DA2-II possesses high similarity (60-80%) with these proteins.
Farah
M F
MF
Departamento de Bioquímica, Universidade Estadual de Campinas, SP, Brazil.
One
M
M
Novello
J C
JC
Toyama
M H
MH
Perales
J
J
Moussatché
H
H
Domont
G B
GB
Oliveira
B
B
Marangoni
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017837:Bothrops; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009893:Opossums / Q000097:blood*
D000997:Antivenins; D001798:Blood Proteins; D003435:Crotalid Venoms