Pyridoxine 4'-alpha- and 5'-alpha-D-glucosides are taken up and metabolized by isolated rat liver cells.
Uptake and metabolism of pyridoxine-4'-alpha-D-glucoside and pyridoxine-5'-alpha-D-glucoside by freshly isolated rat liver cells were studied using the membrane filtration method. The presence of the pyridoxine-alpha-glucosides at either 0.5 or 5.0 micromol/L had no inhibitory effect on the uptake of pyridoxine (0.5 micromol/L) by hepatocytes. The amount of pyridoxine-alpha-glucoside transported into the cells was only 10-12% of that of pyridoxine. The initial rates of uptake of the pyridoxine-alpha-glucosides were linear with concentrations up to 50 micromol/L. On entry into the cells, 78% of pyridoxine-5'-alpha-glucoside and 15% of the pyridoxine-4'-alpha-glucoside were hydrolyzed at the end of 60 min. Because pyridoxine-5'-alpha-glucoside is hydrolyzed readily and does not interfere with the transport of pyridoxine, the glucoside, if present in animal- or plant-derived foods, may be available as a source of vitamin B-6.
Department of Biochemistry, Emory University, Atlanta, GA 30322-3050, USA.
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Joseph
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Tsuge
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Suzuki
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McCormick
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides; D008099:Liver / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014316:Tritium
D005960:Glucosides; D014316:Tritium; C022945:5'-O-(glucopyranosyl)pyridoxine; D011736:Pyridoxine