Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection.
A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of carbohydrate derivatized and determined by CE/LIF was 10 pmol contained in 100 microL of the final reaction mixture. The CE/LIF method provided a linear response for the carbohydrates tested (over 2-3 orders of magnitude), with a limit of detection of 100 amol. Separation efficiencies over 250,000 theoretical plates were obtained. The procedure was used to analyze a tear fluid sample to demonstrate the applicability of the method for the glucose determination in small volumes of biological samples.
Department of Chemistry, State University of New York at Buffalo 14260-3000,
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Perez
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Colón
D003619:Dansyl Compounds / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D007834:Lasers; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis*; D013666:Tears / Q000737:chemistry
D003619:Dansyl Compounds; D004187:Disaccharides; D005456:Fluorescent Dyes; D006834:Hydrazines; D009005:Monosaccharides; C027913:dansyl hydrazine