Characterization of specific anti-Candida IgM, IgA and IgE: diagnostic value in deep-seated infections.
The proposed serological diagnosis of systemic Candida infections is based on a microplate immunocapture technique detecting IgM, IgA and IgE anti-Candida antibodies. Activity is revealed with a suspension of human erythrocytes sensitized with somatic antigen of Candida albicans, and is quantified on an automated plate reader. The sera were obtained from patients with deep-seated (n = 56) and superficial (n = 193) candidosis. We compared this immunological method with a combination of indirect immunofluorescence and co-immunoelectrodiffusion. The immunocapture method was more sensitive (80.4% vs. 48.2% with indirect immunofluorescence and 58.9% with co-immunoelectrodiffusion), and often provided the diagnosis at an earlier stage, with clear therapeutic advantages. The IgA isotype was a particularly valuable marker of deep-seated Candida infections.
Laboratoire de Parasitologie-Mycologie, Ecuipe 4, INSERM U.314, Hôpital Maison Blanche, Reims, France.
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D
Aubert
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D
Puygauthier-Toubas
P
P
Leon
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B
Pignon
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Foudrinier
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Marnef
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Boulant
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JM
Pinon
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000276:immunology; D002177:Candidiasis / Q000175:diagnosis* / Q000276:immunology*; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000097:blood*; D007073:Immunoglobulin E / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007223:Infant; D008297:Male; D051379:Mice; D008875:Middle Aged
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007073:Immunoglobulin E