The WHO multicenter study on Listeria monocytogenes subtyping: random amplification of polymorphic DNA (RAPD).
As part of a WHO multicenter study on Listeria monocytogenes subtyping methods the random amplification of polymorphic DNA (RAPD)-technique was evaluated. Six participants were asked to use a standard protocol to analyse a set of 80 L. monocytogenes strains. This set contained 22 groups of epidemiologically linked isolates and 11 pairs of duplicate strains. Using three different 10-mer primers the median reproducibility of the RAPD-results obtained by the six participants was 86.5% (range 0-100%). Failure in reproducibility was mainly due to results obtained with one particular primer. The number of epidemiological groups found to be homogeneous varied from 1-22 (median 16). However, for some groups an inhomogeneity was found by the majority of participants. The overall correlation between the results from the different participants ranged from 32 to 85%.
Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment, Bilthoven, Netherlands. Karel.Wernars@rivm.nl
K
K
Wernars
P
P
Boerlin
A
A
Audurier
E G
EG
Russell
G D
GD
Curtis
L
L
Herman
N
N
van der Mee-Marquet
D015373:Bacterial Typing Techniques; D008089:Listeria monocytogenes / Q000145:classification*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014944:World Health Organization