Optimization of the reverse transcriptase assay for the detection of viral burden in mice infected with Rauscher murine leukemia virus.
Rauscher murine leukemia virus induces an erythroleukemia in susceptible strains of mice that is associated with splenomegaly and viremia. This animal model has been used for evaluating the in vivo efficacy of potential anti-HIV agents. The in vivo antiviral activity of therapeutic agents has usually been determined by measuring a reduction in the spleen weights of compound-treated mice or by quantitating viremia with the UV-XC plaque assay. The UV-XC assay, however, is time-consuming and labor-intensive. Virions of Rauscher murine leukemia virus, like other retroviruses, contain the enzyme reverse transcriptase. Quantitating the level of this enzyme in infected mouse sera provides a more rapid measure of viremia in the animal. We have examined the effects of several reagents, including detergent, KCl, EGTA, dGMP, spermine, as well as protease and RNase inhibitors, on the reverse transcriptase assay. The optimized assay method was effective in evaluating the antiviral activity of AZT in the Rauscher murine leukemia virus in vivo model. The assay is also amenable to automation if large numbers of assays are required.
Southern Research Institute, Birmingham, AL 35205, USA. white@sri.org
E L
EL
White
L
L
Westbrook
M G
MG
Hollingshead
W M
WM
Shannon
D000818:Animals; D015658:HIV Infections / Q000188:drug therapy; D007942:Leukemia, Experimental; D051379:Mice; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000187:drug effects; D011925:Rauscher Virus / Q000302:isolation & purification*; D012192:Retroviridae Infections; D014412:Tumor Virus Infections; D019562:Viral Load; D015215:Zidovudine / Q000494:pharmacology
D015215:Zidovudine; D012194:RNA-Directed DNA Polymerase