Expression of serotonin transporter mRNA in human brainstem raphe nuclei.
The distribution of the messenger RNA (mRNA) encoding the serotonin transporter was investigated in the entire extent of the ascending raphe systems of the human brainstem, using a sensitive cRNA probe. This mRNA is abundant in the neurons of the raphe nuclei, especially in the dorsal and median raphe. The subregional distribution of this mRNA closely corresponds to that described for tryptophan hydroxylase in immunocytochemical studies. It is expressed at the highest levels in ventral and ventrolateral subregions of the dorsal raphe, with lower levels of expression in the median raphe, oral pontine reticular nuclei, and the supralemniscal cell groups. These results provide a description of the distribution of this mRNA in normal subjects and will serve as the basis for future studies of the subregional distribution of this mRNA in the brains of depressed patients and those who have committed suicide.
McLaughlin
D P
DP
Mental Health Research Institute, University of Michigan Medical School, Ann Arbor, USA.
Little
K Y
KY
López
J F
JF
Watson
S J
SJ
D000328:Adult; D000368:Aged; D000591:Amino Acid Isomerases / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase; D012333:RNA, Messenger / Q000032:analysis*; D011903:Raphe Nuclei / Q000737:chemistry / Q000378:metabolism*; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase