Ultrafast high resolution separation of large DNA fragments by pulsed-field capillary electrophoresis.
Pulsed-field capillary electrophoresis (PFCE) in buffers containing ultradilute polymer solutions is used to separate long chain dsDNA in less than 4 min. Separations are shown to work with chain lengths below 10 kbp and greater than 1.5 Mbp. Several pulse protocols have been examined. If running time is to be minimized, a field inversion with higher peak amplitude in the forward direction than in the reverse, but with equal pulse durations, provides the best resolution. Other protocols can provide higher resolution, but only with longer running times.
Department of Chemistry, University of Michigan, Ann Arbor, USA.
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Kim
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Morris
D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA / Q000302:isolation & purification*; D004574:Electromagnetic Fields; D019075:Electrophoresis, Capillary; D016521:Electrophoresis, Gel, Pulsed-Field; D008970:Molecular Weight; D012150:Polymorphism, Restriction Fragment Length; D013997:Time Factors
D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA