Detection of canine parvovirus antigens with antibodies to synthetic peptides.
Antibodies produced in rabbits against an 18-amino acid peptide (peptide 1, NSLPQSEGATNFGDIGVP) of capsid protein VP2/residues 292-309 of canine parvovirus (CPV) or against an 18-amino acid peptide (peptide 2, GKRNTVLFHGPASTKGKS) of nonstructural protein NS1/residues 391-409 of CPV identified, in immunofluorescence analysis, viral antigens in canine A 72 cells infected with CPV. Antibodies to peptide 2 also identified viral antigens in bovine cells infected with bovine parvovirus. In western blot analysis, antibodies to peptide 1 and peptide 2 also detected viral antigens derived from blue fox parvovirus, feline parvovirus, mink enteritis virus and raccoon dog parvovirus. The peptide antibodies could be used as convenient tools in diagnosis of infections caused by CPV or closely related viruses affecting cats, minks, blue foxes and raccoon dogs.
Department of Biological and Environmental Science, University of Jyväskylä, Finland.
M
M
Vihinen-Ranta
E
E
Lindfors
L
L
Heiska
P
P
Veijalainen
M
M
Vuento
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D036022:Capsid Proteins; D002415:Cats; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D028301:Feline Panleukopenia Virus / Q000145:classification / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D005589:Foxes; D008907:Mink; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000145:classification / Q000276:immunology*; D017993:Parvovirus, Canine / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D011817:Rabbits; D011821:Raccoons
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins