Discrimination between the immature stages of Ceratitis capitata and Anastrepha fraterculus (Diptera:Tephritidae) populations by random amplified polymorphic DNA polymerase chain reaction.
A random amplified polymorphic DNA polymerase chain reaction method using 10-base long commercial random primers was used to identify polymorphisms in Argentine populations of 2 fruit fly pests. A fast and reliable discrimination between Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and Anastrepha fraterculus (Wiedemann) immature or mature stages was obtained using as few as 1 insect per assay. The population of origin of immature individuals can be identified unambiguously using 2 primers.
Instituto de Investigaciones Bioquímicas Fundación Campomar, Buenos Aires, Argentina.
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Sonvico
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Manso
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LA
Quesada-Allue
D000818:Animals; D004175:Diptera / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique