Prevention of bacterial adherence to implant surfaces with a crosslinked albumin coating in vitro.
Titanium surfaces were coated with bovine serum albumin using carbodiimide, a crosslinking agent. The durability of the coated surfaces and the inhibitory effect of the albumin coating on bacterial adherence were tested in vitro for 20 consecutive days at 37 degrees C in phosphate buffered saline, with intermittent agitation. The results showed that only 10% of the coated bovine serum albumin decayed off the surface during the 20-day incubation period. The inhibition rate of the albumin coating on bacterial adherence remained high (greater than 8.5%) throughout the experiment. The results suggested potential use of this crosslinked albumin coating to reduce bacterial adherence and thus the subsequent possibility of prosthetic or implant infection in vivo.
An
Y H
YH
Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston 29425, USA.
Stuart
G W
GW
McDowell
S J
SJ
McDaniel
S E
SE
Kang
Q
Q
Friedman
R J
RJ
D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008422:Materials Testing; D008828:Microbiological Techniques; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology*; D014025:Titanium
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D012709:Serum Albumin; D014025:Titanium