Comparison of anterior chamber infusates on the intraocular pressure of intact rat eyes.
1. Infusions of balanced salt solutions (BSS) into the eye often cause a delayed, gradual increase in intraocular pressure or outflow facility, known as the "washout" effect. The reason(s) is occult, especially at low input rates when drainage mechanisms are not overloaded by excessive volume input. However, direct, quantitative comparisons of BSSs used in ocular research have been reported infrequently. 2. We compared the effects of three BSSs on intraocular pressure and the estimated resistance to drainage after a 1-hr, low-volume infusion into the anterior chambers of the eyes of anesthetized rats. 3. The BSSs tested raised intraocular pressure (P < 0.05) after a 20-40-min delay, and the highest IOPs occurred at 1 hr. Recovery of intraocular pressure to baseline only occurred with one BSS (Dulbecco). 4. Fitting the ascending and descending portions of the mean pressure curves to an exponential revealed differences among the infusates. The Dulbecco solution resulted in minimal changes in time constant, gain, and offset during the ascending and descending periods. 5. The data obtained show that different BSSs yield pressure curves that appear grossly similar, even though there were large differences in composition and osmolality. However, the underlying changes in ocular dynamics were not identical. Thus, it may be prudent to test more than one solvent to study "washout," or to deliver drugs directly into the anterior chamber.
Searles
R V
RV
Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey 17033, USA.
Balaban
C D
CD
Severs
W B
WB
D000818:Animals; D000867:Anterior Chamber; D007429:Intraocular Pressure; D008297:Male; D014677:Pharmaceutical Vehicles; D051381:Rats; D017207:Rats, Sprague-Dawley
D014677:Pharmaceutical Vehicles