Differential modulation of natural and adaptive immunity in Fischer rats exposed for 6 weeks to 60 Hz linear sinusoidal continuous-wave magnetic fields.
Two separate, independent experiments were conducted to evaluate the effect of 60 Hz linearly polarized, sinusoidal, continuous-wave magnetic fields (MFs) on immune system performances in rats born and raised under these fields. Each experiment lasted for 6 weeks. A total of 96 animals, divided into groups of eight animals each, was exposed for 20 h/day to MFs of different intensities, i.e., sham (< 0.02 microT) and 2, 20, 200, and 2000 microT. Another group of animals, which was housed in a separate room, served as cage controls (CC). These animals were exposed to ambient MFs of < 0.02 microT. The following immune responses were evaluated in both experiments total T and B cells; CD4+ and CD8+ subpopulation and natural killer (NK) cell activity in splenic lymphocytes; hydrogen peroxide (H2O2), nitrous oxide (NO), and tumor necrosis factor (TNF) production by peritoneal macrophages. Our results show that a 6 week exposure to MFs induced a significant decrease in the number of CD5+, CD4+, and CD8+ populations. These changes were even more significant in rats that were exposed to fields of 2000 microT. A lower, although significant, decrease in the CD5+ population was also observed in animals that were exposed to fields of 200 microT. Linear regression analysis demonstrated a dose effect with MF intensity. B lymphocyte (Ig+ cell) populations also showed a 12% decrease (P < .05) in the groups that were exposed to fields of 20 and 200 microT. However, these results were not significant, and no relation with MF intensities could be demonstrated. In contrast, evaluation of splenic NK cell activity revealed a 50% increase (P < .05) in animals that were exposed to fields of 2000 microT. No significant results were obtained from the evaluation of TNF activity and NO secretion in peritoneal macrophages. Phorbol 12-myristate 13-acetate (PMA)-stimulated and net H2O2 productions for a minor subpopulation of peritoneal cells showed positive dose-response correlations by linear regression analysis. Taken together, our results suggest that an in vivo exposure of rats for 6 weeks to 60 Hz MFs can induce significant immunological perturbations on effector cells of both natural and adaptive immunity in a dose-dependent fashion.
Immunology Research Center, Institut Armand-Frappier, Laval, Québec, Canada.
L
L
Tremblay
M
M
Houde
G
G
Mercier
J
J
Gagnon
R
R
Mandeville
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D002940:Circadian Rhythm; D004781:Environmental Exposure; D006861:Hydrogen Peroxide / Q000378:metabolism; D007111:Immunity, Cellular / Q000502:physiology*; D007113:Immunity, Innate / Q000502:physiology*; D007694:Killer Cells, Natural / Q000502:physiology; D016014:Linear Models; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008280:Magnetics; D009609:Nitrous Oxide / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014409:Tumor Necrosis Factor-alpha; D006861:Hydrogen Peroxide; D009609:Nitrous Oxide; D013755:Tetradecanoylphorbol Acetate