Mouse multivalent IgG(2a, b) antibody--ricin A-chain immunotoxins combined with homologous or heterologous interleukin 2 in the treatment of a murine malignant lymphoproliferation (EL4).
Two immunotoxins containing ricin A-chain, staphylococcal protein A and mouse anti-Thy 1.2 antibodies of IgG(2a,b) subclass, were prepared. The two multivalent immunotoxins of 750 and 370 kDa and molar ratio A-chain: IgG of 1:2, were used for the treatment of mice bearing ascitic EL4 lymphoma. The immunotoxin-treatment, performed intraperitoneally, was combined or not with homologous or heterologous interleukin 2. The antitumor effects expressed by increase of mice survival time (p < 0.001 as compared with nontreated animals) corresponded to a proportion of 88-90% lymphoma cell-kill by immunotoxin and 93-95% by combination treatment (immunotoxin + interleukin 2), as calculated from the relationship between the survival time of nontreated mice and the number of tumor cells inoculated.
Center of Immunology, Stefan Nicolau Institute of Virology, Bucharest, Romania.
G
G
Mota
R
R
Marches
V
V
Cialacu
V
V
Roman
E
E
Kozma
M
M
Mărgineanu
S
S
Dima
I I
II
Moraru
D000818:Animals; D004357:Drug Synergism; D007074:Immunoglobulin G / Q000145:classification* / Q000627:therapeutic use*; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D000922:Immunotoxins / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000145:classification / Q000627:therapeutic use*; D007945:Leukemia, Lymphoid / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D012276:Ricin / Q000627:therapeutic use*; D013205:Staphylococcal Protein A / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D000922:Immunotoxins; D007376:Interleukin-2; D013205:Staphylococcal Protein A; D012276:Ricin