[Non-specific elevation of anti-dsDNA antibody measured by Farr method found in a patient with liver cirrhosis].
In a patient with liver cirrhosis, an elevation of serum anti-dsDNA antibody titer (100-193.3 IU/ml) measured by Farr method was found. This anti-dsDNA antibody was not identified by Crithidia Luciliae method, and the analysis using ELISA method showed that the DNA-antibody is an anti-ssDNA antibody (IgG) and that it is reactive only with ssDNA by the suppression test using purified DNA with lambda phage. Based on this finding, the high titer of anti-dsDNA antibody found in this patient using Farr method was considered to be non-specific cross-reaction with ssDNA.
Central Clinical Laboratory, Kanazawa University Hospital.
T
T
Takamura
M
M
Nagata
T
T
Hashimoto
M
M
Takamura
K
K
Kobayashi
D000974:Antibodies, Antinuclear / Q000097:blood*; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000276:immunology*; D008875:Middle Aged; D011863:Radioimmunoassay
D000974:Antibodies, Antinuclear; D004247:DNA