The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs.
We have studied the role of E-selectin in leukocyte accumulation into Ag-specific cutaneous delayed-type hypersensitivity reactions in pigs sensitized to the topical application of 2,4-dinitro-1-fluorobenzene or to the intradermal injection of bacillus Calmette-Guérin. The delayed-type hypersensitivity reactions were shown to be specific for the sensitizing Ag and characterized by the up-regulation of E-selectin, as demonstrated by the uptake of tracer 99mTc-labeled monoclonal anti-E-selectin mAb and entry of 51Cr-labeled PBL and (111)In-labeled polymorphonuclear cells (PMN). Intravenous injection of 5 mg/kg of a F(ab')2 preparation of a monoclonal anti-E-selectin Ab at peak times of leukocyte entry resulted in a significant inhibition of entry of both PMN and lymphocytes. The anti-E-selectin Ab inhibited PMN recruitment by 70 to 90% and lymphocyte recruitment by 50 to 60%. In comparison, an anti-CD18 treatment reduced PMN recruitment by 70 to 90% and lymphocyte recruitment by 60 to 70% in this model. These data confirm an important role for E-selectin in the recruitment of both PMN and lymphocytes to sites of immune-based dermal inflammation.
Department of Immunology, The Babraham Institute, Cambridge, United Kingdom.
R M
RM
Binns
A
A
Whyte
S T
ST
Licence
A A
AA
Harrison
Y T
YT
Tsang
D O
DO
Haskard
M K
MK
Robinson
D000367:Age Factors; D000818:Animals; D018821:CD18 Antigens / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000276:immunology; D019040:E-Selectin / Q000502:physiology*; D004730:Endothelium, Vascular / Q000503:physiopathology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D013552:Swine; D014373:Tuberculin / Q000276:immunology
D018821:CD18 Antigens; D019040:E-Selectin; D014373:Tuberculin; D004139:Dinitrofluorobenzene