Estimations of average particle sizes and size distributions of commercially available human-insulin aqueous suspensions using laser-light diffraction spectroscopy.
The average diameter and size distribution of particles in commercially available isophane-insulin and zinc-insulin aqueous suspensions (11 products) were examined using laser-light diffraction spectroscopy (LLDS). Wide variation was observed between products in volume-weighted and number-weighted average diameters. The particles in the insulin aqueous suspensions were found to be mono-dispersed within relatively narrow size distributions. Sonication for a short time led to a downshift in the apparent average diameter, which was most remarkable with isophane-insulin aqueous suspensions. This could be ascribed to dispersion of aggregated needle-like crystals and/or crystal breakage. In the case of biphasic isophane-insulin aqueous suspensions, the average size as well as the size distribution shifted toward the smaller end of the scale with decrease in the isophane content.
Division of Drugs, Osaka Branch, National Institute of Health Sciences, Japan.
H
H
Komatsu
A
A
Kitajima
Y
Y
Nakata
S
S
Okada
D006801:Humans; D007336:Insulin, Isophane / Q000737:chemistry*; D049528:Insulin, Long-Acting / Q000737:chemistry*; D007834:Lasers; D009995:Osmosis; D010316:Particle Size; D012542:Scattering, Radiation; D013535:Suspensions
D049528:Insulin, Long-Acting; D013535:Suspensions; D007336:Insulin, Isophane