Biocompatibility of sulphonated polyurethane surfaces.
Surfaces of medical devices made of polymeric materials may promote thrombosis and inflammation. Therefore, in an attempt to produce surfaces which might diminish biomaterial-mediated thrombosis and inflammation, surface derivatization with 2-acrylamido-2-methylpropanesulphonic acid (AMPS) was carried out. The derivatization procedure generates free radicals which initiate the copolymerization of AMPS monomers directly to a polyurethane surface. In an in vitro blood loop study using non-anticoagulated human blood, the resulting AMPS-derivatized material completely abrogates the generation of fibrinopeptide A, decreases the production of beta-thromboglobulin and C3a, and decreases the adherence of platelets. The derivatized material also attracts fewer adherent neutrophils when implanted in mice. However, AMPS derivatization unexpectedly increases the recruitment of macrophages to implanted material and promotes the formation of adherent sleeve thrombi on central venous catheters indwelling in non-anticoagulated canine femoral veins. Thus, AMPS derivatization has highly variable effects on inflammatory and thrombotic systems. Further investigation is clearly required to determine the mechanisms underlying both desired and adverse effects.
Center for Biomaterials Research, Medtronic, Inc., Minneapolis, MN 55430, USA.
J R
JR
Keogh
M F
MF
Wolf
M E
ME
Overend
L
L
Tang
J W
JW
Eaton
D000178:Acrylamides / Q000737:chemistry / Q000494:pharmacology*; D000476:Alkanesulfonates / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D002448:Cell Adhesion / Q000187:drug effects; D015926:Complement C3a / Q000378:metabolism; D004285:Dogs; D005268:Femoral Vein / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D010586:Phagocytes / Q000166:cytology / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry / Q000378:metabolism*; D019736:Prostheses and Implants; D013499:Surface Properties; D013927:Thrombosis / Q000139:chemically induced / Q000517:prevention & control*; D001620:beta-Thromboglobulin / Q000378:metabolism
D000178:Acrylamides; D000476:Alkanesulfonates; D001672:Biocompatible Materials; D011140:Polyurethanes; D001620:beta-Thromboglobulin; C056288:2-acrylamido-2-methylpropanesulfonate; D015926:Complement C3a