Insulin-like growth factor binding protein 2 is differentially expressed in leukaemic B- and T-cell lines.
Production of insulin-like growth factor binding protein (IGFBP)-2 and accumulation of IGFBP-2 mRNA was determined in six leukaemic T-, B- or promyelocytic cell lines. Cell growth was compared in serum free medium M-3 and in medium M-9 containing 5% FCS. In both media, high amounts of IGFBP-2 as measured by radioimmunoassay were detectable in culture supernatant of T-cell lines and promyelocytic HL-60 cells, whereas only small amounts of IGFBP-2 were secreted by the B-cell lines. Production of IGFBP-2 in M-9 was approximately 20-fold higher (up to 195 ng ml-1) than in M-3, partially reflecting higher proliferation. However, quantitative reverse transcriptase polymerase chain reaction analysis revealed that, independent of the culture medium 10(6) T-cells contained between 30 and 48 units IGFBP-2 mRNA relative to the glycerol aldehyde phosphate dehydrogenase control gene, but B-cells contained less than 1 unit. Since IGF-II is known to be a major regulator of IGFBP-2, its influence on IGFBP-2 expression has to be investigated.
Children's Hospital, University, Tübingen, Germany.
M W
MW
Elmlinger
K
K
Wimmer
E
E
Biemer
W F
WF
Blum
M B
MB
Ranke
G E
GE
Dannecker
D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000032:analysis / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis; D007700:Kinetics; D015448:Leukemia, B-Cell; D015473:Leukemia, Promyelocytic, Acute; D015458:Leukemia, T-Cell; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay; D013997:Time Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D018971:Insulin-Like Growth Factor Binding Protein 2; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases