Protective action of hydroxyethyl rutosides on singlet oxygen challenged cardiomyocytes.
1. The effect of a standardized mixture of beta-hydroxyethyl rutosides against oxidative damage in singlet oxygen-challenged isolated cardiac myocytes from adult rats was investigated. The morphology of the myocytes was evaluated as an indicator for cell viability (elongated, rod shaped cells vs. hypercontracted, rounded cells). The determination of the production of thiobarbituric acid reactive substances served as an indicator for lipid peroxidation. 2. Exposure to singlet oxygen which was generated by photo-excitation of rose bengal (10(-7) M) reduced the number of rod shaped (vital) cardiomyocytes by 78.5 +/- 2.5% and increased the production of thiobarbituric acid reactive substances by 1180 +/- 150% in comparison to incubation with control buffer. 3. Coincubation of the cells with beta-hydroxyethyl rutosides (concentration range: 6.7 pg ml-1 to 670 micrograms ml-1) increased the number of rod shape cardiomyocytes after exposure to singlet oxygen in a dose-dependent bell-shaped manner. A significant protective effect was observed at beta-hydroxyethyl rutosides concentrations ranging from 0.67 ng ml-1 to 67 ng ml-1. 4. In spite of their protective action, beta-hydroxyethyl rutosides did not reduce the accumulation of thiobarbituric acid reactive substances, used as an indicator for lipid peroxidation. 5. The data suggest that beta-hydroxyethyl rutosides exert a protective action against oxygen radical-induced damage of cardiac myocytes at very low concentrations without interfering with lipid peroxidation.
Zentrum der Inneren Medizin, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
H G
HG
Olbrich
P
P
Grabisch
A
A
Grossmann
T
T
Rinne
H
H
Klepzig
E
E
Mutschler
D000818:Animals; D006321:Heart / Q000187:drug effects*; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology*; D010100:Oxygen; D010777:Photochemistry; D051381:Rats; D012395:Rose Bengal / Q000494:pharmacology; D026082:Singlet Oxygen
D006896:Hydroxyethylrutoside; D026082:Singlet Oxygen; D012395:Rose Bengal; D010100:Oxygen