Are proteasomes involved in the formation of the kinetochore?
Proteasomes catalyse the degradation of proteins responsible for the regulation of mitosis enabling the cell to complete cell division. We have studied the effect of an inhibitor of the chymotrypsin-like activity of the proteasome on the trilaminar structure of the kinetochore in HeLa cells. Whereas a role for the proteasome in the degeneration of the kinetochore was predicted, we found instead that the inhibitor strongly regarded kinetochore development. We observed different 'developmental' stages of the kinetochore from the fibrous ball of a 'prekinetochore' to the 'mature' kinetochore in one cell. The data presented here support the proposition that proteasomes are involved in kinetochore formation.
German Cancer Research Center, Division 0430, Heidelberg.
N
N
Paweletz
C
C
Wójcik
D
D
Schroeter
E M
EM
Finze
D002453:Cell Cycle; D002503:Centromere / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D016213:Cyclins / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000502:physiology*; D017666:Deuterium Oxide / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000648:ultrastructure*; D008677:Metaphase; D009097:Multienzyme Complexes / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex
D016213:Cyclins; D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D017666:Deuterium Oxide; D003078:Colchicine