Temporal specificity of muscarinic synaptic modulation of the Ca(2+)-dependent K+ current (ISAHP) in rat hippocampal neurones.
1. We examined synaptic modulation of the Ca(2+)-dependent K+ current (ISAHP), which underlies the slow after-hyperpolarization (sAHP) in hippocampal CA1 neurones of rat brain slices. ISAHP was evoked in whole-cell voltage-clamp mode by depolarizing pulses, and synaptic afferents to CA1 neurones were stimulated electrically with a paired-pulse protocol. 2. Afferent stimulation delivered 200-1500 ms prior to be depolarizing pulse produced a profound reduction of ISAHP by 58%, but not other Ca(2+)-dependent outward currents that preceded ISAHP. Perfusion of slices with atropine significantly attenuated the synaptic reduction of ISAHP, indicating an event mediated largely by muscarinic receptor activation. When delivered < 400 ms after the depolarizing pulse, similar synaptic stimuli produced no substantial reduction in ISAHP, even in neurons where the duration of ISAHP was prolonged to 8-10 s either by lowering the recording temperature or by intracellular application of a calcium chelator. 3. To examine the effect of cholinergic stimulation of the depolarization-activated Ca2+ influx, high-threshold voltage-activated Ca2+ currents were recorded in the conventional or perforated whole-cell mode. Perfusion of slices with 5-10 microM carbachol for 5-10 min caused no substantial decrease in these Ca2+ currents, suggesting that the synaptic reduction of ISAHP is unlikely to be due to a blockade of depolarization-induced Ca2+ influx which triggers the generation of ISAHP. 4. The present data demonstrate that afferent stimulation reduces ISAHP only if it occurs prior to the depolarization-induced Ca2+ influx. We propose that modulation of inactive sAHP channels by muscarinic stimulation may decrease their sensitivity to the influx of Ca2+, whereas sAHP channels activated by Ca2+ may compete with the receptor-coupled modulation thus rendering the sAHP channels unresponsive to cholinergic afferent stimulation.
Zhang
L
L
Toronto Hospital Research Institute, Department of Medicine (Neurology), University of Toronto, Canada. zhang@camtwh.eric.on.ca
Han
D
D
Carlen
P L
PL
D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D004594:Electrophysiology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D017777:Parasympathetic Fibers, Postganglionic / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D013997:Time Factors
D015220:Calcium Channels; D015221:Potassium Channels; D011976:Receptors, Muscarinic; D002118:Calcium