Rapid determination of amphotericin B in serum and urine by third-order derivative spectrophotometry.
A derivative spectrophotometric method for rapid monitoring of amphotericin B in serum and urine down to 30 ng/mliters is described. Samples are treated with acetonitrile, and amphotericin B is directly quantified in the crude extracts on the basis of the intensity of the peak that appears at 402 nm when the normal absorption spectrum is submitted to third-order derivative processing. Accuracy data suggested recoveries in the range of 84.3-94.9% for serum and 85.6-93.4% for urine. The precision of the method was better than 11.3% for serum and 9.2% for urine when samples contained as low as 29.6 ng/mliters of amphotericin B. Ease of applicability, short analysis time, low cost, and reliability are the main advantages of the method.
Laboratory of Nutrition, Aristotle University, Thessaloniki, Greece.
N A
NA
Botsoglou
D J
DJ
Fletouris
G E
GE
Papageorgiou
P
P
Florou-Paner
A J
AJ
Mantis
D000666:Amphotericin B / Q000097:blood* / Q000592:standards / Q000652:urine*; D016903:Drug Monitoring / Q000379:methods; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D000666:Amphotericin B