Evaluation of a novel stable whole blood quality control material for lymphocyte subset analysis: results from the UK NEQAS immune monitoring scheme.
The UK NEQAS immune Monitoring Scheme (UK NEQAS) evaluates the performance of laboratories routinely performing T-lymphocyte subset analysis on HIV-infected individuals. The scheme originally issued fresh whole blood, but a significant problem was that of analyte stability, especially 36 h postphlebotomy. To circumvent this problem, we have developed a novel stabilisation procedure that ensures retention of leucocyte light scatter and immunological staining characteristics for up to 300 days. In addition, the stabilised whole blood preparation is fully compatible with flow cytometer technology, incorporating either whole blood lysis or "no wash, no lyse" techniques. The ranges of interlaboratory coefficient of variation for the stabilised material are now tighter than those previously obtained with fresh whole blood. Development of this novel material has enabled overseas laboratories to participate in the UK NEQAS immune Monitoring Scheme and could in the future lead to the production of reference and/or calibration reagents for leucocyte immunophenotyping.
UK NEQAS Leucocyte Immunophenotyping Schemes, Department of Haematology, Northern General Hospital NHS Trust, Sheffield, England.
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D
Barnett
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V
Granger
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P
Mayr
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I
Storie
G A
GA
Wilson
J T
JT
Reilly
D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001769:Blood; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000592:standards; D006461:Hemolysis; D006801:Humans; D016130:Immunophenotyping / Q000592:standards*; D007755:Laboratories, Hospital / Q000592:standards; D016131:Lymphocyte Subsets; D010865:Pilot Projects; D011786:Quality Control; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006113:United Kingdom