Stimulation of sarcoplasmic reticulum Ca(2+)-ATPase by gingerol analogues.
We have reported previously that [8]-gingerol increased Ca(2+)-ATPase activity. Here we synthesized gingerol related compounds (AP-004, AP-005 and AP-015) and investigated the effects of gingerols ([6]-gingerol, [8]-gingerol and [10]-gingerol) and the synthesized compounds on the Ca(2+)-ATPase activity of sarcoplasmic reticulum (SR). The Ca(2+)-ATPase activity and the Ca(2+)-pumping activity increased by these compounds in a concentration-dependent manner. It is probable that both the o-methoxyphenol and hydrocarbon chain in the molecule of gingerol analogues are necessary for the activation of the Ca(2+)-pumping ATPase activity of SR.
Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Y
Y
Ohizumi
S
S
Sasaki
K
K
Shibusawa
K
K
Ishikawa
F
F
Ikemoto
D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002396:Catechols; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D009153:Mutagens / Q000737:chemistry / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D010946:Plants, Medicinal; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D002396:Catechols; D005233:Fatty Alcohols; D009153:Mutagens; C007845:gingerol; D000252:Calcium-Transporting ATPases; D002118:Calcium