Quantitative PCR with colorimetric detection.
We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of PCRs made with various amounts of standard. This also enables the control of the consistency of the data and elimination of some artifactual results.
Demay
F
F
Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris.
Darche
S
S
Kourilsky
P
P
Delassus
S
S
D000199:Actins / Q000032:analysis / Q000235:genetics; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000199:Actins; D016753:Interleukin-10