Improved method for producing neuronal hybrids using emetine and actinomycin D.
We modified the method of somatic cell fusion for neurons to improve the efficiency of hybrid production. C1300 neuroblastoma cells were incubated with emetine and actinomycin D before fusion. After fusion between C1300 cells and adult mouse dorsal root ganglia neurons with polyethyleneglycol, we were able to select the hybrids from non-fused cells. We obtained hybrid clones at high efficiency (7.2 clones/10(4) neurons).
Department of Physiology, School of Medicine Yokohama City University, Japan.
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Hikawa
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Takenaka
D000818:Animals; D002459:Cell Fusion; D003584:Cytological Techniques; D003609:Dactinomycin / Q000494:pharmacology*; D004640:Emetine / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology; D006822:Hybrid Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D019384:Nucleic Acid Synthesis Inhibitors; D003609:Dactinomycin; D004640:Emetine