Interleukin-10 gene expression induced by HIV-1 Tat and Rev in the cells of HIV-1 infected individuals.
The role of cytokines in the regulation and function of the immune system is of great importance. In human immunodeficiency virus (HIV) infection, with progressive deterioration of cell-mediated immune response, cytokines are dysregulated. We have therefore investigated cytokine mRNA expression in type-1 and type-2 helper T cells of HIV-seropositive (HIV+) individuals, stimulated with mitogen (leukoagglutinin) and HIV-1 Tat and Rev peptides, previously found to induce proliferative T-cell responses in these individuals. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect interleukin 2 (IL-2), interferon gamma (IFN-gamma), IL-4, and IL-10 mRNAs. There was no difference in the mRNA expression of these cytokines when the cells of HIV-infected or noninfected individuals were polyclonally stimulated with the mitogen, as all cytokine mRNAs were detected in both groups. Baseline cytokine expression of unstimulated cells was, however, different in these two groups: the cells of HIV+ persons did not show comparable expression of mRNAs to HIV-seronegative (HIV+) individuals. When the cells of HIV+ individuals were stimulated with the peptides, 70% of the cases showed IL-10 mRNA expression, 20% IFN-gamma, and 10% IL-2, with no detection of IL-4 mRNA in any of the cases. Our results thus show that HIV-specific T-cell antigens induce production of IL-10 in HIV-infected individuals. The increase in IL-10 demonstrated here may have a role in hyperactivation of B cells, as well as in immunosuppression of T cells often seen in HIV-infected individuals.
Institute of Medical Technology, University of Tampere, Finland.
V
V
Blazevic
M
M
Heino
A
A
Lagerstedt
A
A
Ranki
K J
KJ
Krohn
D000199:Actins / Q000378:metabolism; D016827:CD8 Antigens / Q000032:analysis; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D016373:DNA, Antisense; D005786:Gene Expression Regulation; D015694:Gene Products, rev / Q000494:pharmacology*; D015696:Gene Products, tat / Q000494:pharmacology*; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D010835:Phytohemagglutinins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D000199:Actins; D016827:CD8 Antigens; D017931:DNA Primers; D016373:DNA, Antisense; D015694:Gene Products, rev; D015696:Gene Products, tat; D007376:Interleukin-2; D010835:Phytohemagglutinins; D012333:RNA, Messenger; C100156:leukoagglutinins, plants; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma