Oncogene alterations in primary, recurrent, and metastatic human bone tumors.
We investigated the structure and the expression of various oncogenes in three of the most common human bone tumors-osteosarcoma (36 samples from 34 patients), giant cell tumor (10 patients), and chondrosarcoma (18 patients)-in an attempt to identify the genetic alterations associated with these malignancies. Alterations of RB and p53 were detected only in osteosarcomas. Alterations of c-myc, N-myc, and c-fos were detected in osteosarcomas and giant cell tumors. Ras alterations (H-ras, Ki-ras, N-ras) were rare. Chondrosarcomas did not contain any detectable genetic alterations. Our results suggest that alterations of c-myc, N-myc, and c-fos oncogenes occur in osteosarcomas, in addition to those previously described for the tumor suppressor genes RB and p53. Moreover, statistical analyses indicate that c-fos alterations occur more frequently in osteosarcoma patients with recurrent or metastatic disease.
Department of Pathology, University of Chicago, Illinois 60637, USA.
F
F
Pompetti
P
P
Rizzo
R M
RM
Simon
B
B
Freidlin
D J
DJ
Mew
H I
HI
Pass
P
P
Picci
A S
AS
Levine
M
M
Carbone
D000293:Adolescent; D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D002648:Child; D002813:Chondrosarcoma / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D005870:Giant Cell Tumors / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D016159:Tumor Suppressor Protein p53