Neurofilament subunit NF-H modulates axonal diameter by selectively slowing neurofilament transport.
To examine the mechanism through which neurofilaments regulate the caliber of myelinated axons and to test how aberrant accumulations of neurofilaments cause motor neuron disease, mice have been constructed that express wild-type mouse NF-H up to 4.5 times the normal level. Small increases in NF-H expression lead to increased total neurofilament content and larger myelinated axons, whereas larger increases in NF-H decrease total neurofilament content and strongly inhibit radial growth. Increasing NF-H expression selectively slow neurofilament transport into and along axons, resulting in severe perikaryal accumulation of neurofilaments and proximal axonal swellings in motor neurons. Unlike the situation in transgenic mice expressing modest levels of human NF-H (Cote, F., J.F. Collard, and J.P. Julien. 1993. Cell. 73:35-46), even 4.5 times the normal level of wild-type mouse NF-H does not result in any overt phenotype or enhanced motor neuron degeneration or loss. Rather, motor neurons are extraordinarily tolerant of wild-type murine NF-H, whereas wild-type human NF-H, which differs from the mouse homolog at > 160 residue positions, mediates motor neuron disease in mice by acting as an aberrant, mutant subunit.
Division of Cellular and Molecular Medicine, University of California at San Diego, La Jolla 92093, USA.
J R
JR
Marszalek
T L
TL
Williamson
M K
MK
Lee
Z
Z
Xu
P N
PN
Hoffman
M W
MW
Becher
T O
TO
Crawford
D W
DW
Cleveland
D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000502:physiology*; D001692:Biological Transport; D016923:Cell Death; D005727:Ganglia, Spinal / Q000737:chemistry; D018628:Gene Dosage; D015870:Gene Expression; D007382:Intermediate Filaments / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000473:pathology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000473:pathology; D009186:Myelin Sheath; D009410:Nerve Degeneration; D016900:Neurofilament Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D012584:Sciatic Nerve / Q000737:chemistry; D013116:Spinal Cord / Q000737:chemistry / Q000473:pathology; D019076:Transgenes / Q000235:genetics; D014404:Tubulin / Q000032:analysis
D016900:Neurofilament Proteins; D012333:RNA, Messenger; D014404:Tubulin; C051774:neurofilament protein H; C061195:neurofilament protein M