Determination of naringin and naringenin in human plasma by high-performance liquid chromatography.
An HPLC method for determining a flavonoid, naringin, and its metabolite, naringenin, in human plasma is presented for application to the pharmacokinetic study of naringin. Isocratic reversed-phase HPLC was employed for the quantitative analysis by using genistin (for naringin) or diadzein (for naringenin) as an internal standard and solid-phase extraction using s Sep-Pak t C18 cartridge. For the determination, HPLC was carried out using an Inertsil ODS-2 column (250 x 4.6 m I.D., 5 microns particle size). The mobile phases were acetonitrile-0.1 M ammonium acetate solution (20:80, v/v; pH 7.1) for naringin and acetonitrile-0.1 M ammonium acetate solution-acetic acid (30:69:1, v/v; pH 4.9) for naringenin. The flow-rate was 1 ml min-1. The analyses were performed by monitoring the wavelength of maximum UV absorbance at 280 nm for naringin and at 292 nm for naringenin. The detection limits on-column were about 0.2 ng for the two flavonoids.
Kyorin University, School of Health Sciences, Tokyo, Japan.
K
K
Ishii
T
T
Furuta
Y
Y
Kasuya
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; C005273:naringenin; C005274:naringin