Fibroblast growth factor receptor 1 in skeletal and heart muscle cells: expression during early avian development and regulation after notochord transplantation.
Basic fibroblast growth factor (bFGF, FGF-2) mediates several biological functions during embryonic development. With regard to skeletal muscle formation, it has been suggested that FGF-2 is involved in the growth and differentiation of myogenic precursor cells. To identify the FGF-responsive cells we studied the expression of FGF receptor type I (FGFR-1) during early embryonic development of the chick. FGFR-1 immunoreactivity is present at all stages examined (embryonic day [E] 2-E5). Expression of FGFR-1 is found in the somite myotome, limb bud muscle cells, eye and tongue muscle cells, and myocardium. Transplantation of an additional notochord into the paraxial mesoderm, which prevents the formation of a myotome, reveals the absence of FGFR-1 immunoreactivity on the operated side. The distinct expression pattern of FGFR-1 in migrating and differentiating muscle cells indicates that in addition to the stimulation of proliferation of myoblasts, FGF-2 exerts other (nonmitogenic) effects on postmitotic myocytes.
Institute of Anatomy, University of Freiburg, Germany.
C
C
Grothe
B
B
Brand-Saberi
J
J
Wilting
B
B
Christ
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002642:Chick Embryo; D003370:Coturnix / Q000196:embryology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004627:Embryonic Induction; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D017403:In Situ Hybridization; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009672:Notochord / Q000637:transplantation*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D017468:Receptors, Fibroblast Growth Factor; D014157:Transcription Factors; C068686:Pax3 protein, mouse; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1