Lack of inhibitory effect of benzyl isothiocyanate on 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP)-induced mammary carcinogenesis in rats.
Modifying effects of benzyl isothiocyanate (BITC), a glucosinolate compound, were investigated on 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced mammary carcinogenesis in female SD rats. One hundred twenty five female rats were divided into 4 groups. Starting at 6 weeks of age, rats were fed high fat diet containing 23.5% corn oil (Groups 1 and 4) or the experimental diet (high fat diet with 400 ppm BITC) (Groups 2 and 3). At 7 weeks of age, Groups 1 and 2 were given PhIP (100 mg/kg body weight, 8 times for 16 days) by intragastric tube. One week after the final PhIP treatment, the experimental diet for Groups 2 and 3 switched to high fat diet. At the termination (31 weeks after the start of experiment), mammary adenocarcinomas were recognized in Group 1 (PhIP alone) and Group 2 (PhIP + BITC). The incidence of neoplasms was 74.2% in Group 1, and 62.5% in Group 2. The multiplicity of them was 1.71 +/- 1.70 in Group 1, and 1.91 +/- 2.94 in Group 2. Mean sizes of tumors were 9.9 +/- 7.1 mm in Group 1, and 10.8 +/- 6.8 mm in Group 2. But no significant differences for tumors were available between the two groups. These results imply that the glucosinolate compound does not have clear inhibitory effects on PhIP-induced mammary carcinogenesis in rats.
Department of Pathology, Gifu University School of Medicine, Japan.
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Ino
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Sugie
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Ohnishi
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Mori
D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D003043:Cocarcinogenesis; D004041:Dietary Fats / Q000009:adverse effects; D005260:Female; D007093:Imidazoles / Q000633:toxicity*; D017879:Isothiocyanates / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D004041:Dietary Fats; D007093:Imidazoles; D017879:Isothiocyanates; C031403:benzyl isothiocyanate; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine