A systemic antiviral resistance-inducing protein isolated from Clerodendrum inerme Gaertn. is a polynucleotide:adenosine glycosidase (ribosome-inactivating protein).
Two systemic antiviral resistance-inducing proteins, CIP-29 and CIP-34, isolated from Clerodendrum inerme Gaertn. leaves, were tested for ribosome-inactivating properties. It was found that CIP-29 has the characteristics of a polynucleotide:adenosine glycosidase (ribosome-inactivating protein), in that it inhibits protein synthesis both in cell-free systems and, at higher concentrations, in cells, and releases adenine from ribosomes, RNA, poly(A) and DNA. As compared with other known RIPs, CIP-29 deadenylates DNA at a high rate, and induces systemic antiviral resistance in susceptible plants.
Dipartimento di Patologia Sperimentale dell'Università di Bologna, Italy.
F
F
Olivieri
V
V
Prasad
P
P
Valbonesi
S
S
Srivastava
P
P
Ghosal-Chowdhury
L
L
Barbieri
A
A
Bolognesi
F
F
Stirpe
D000225:Adenine / Q000378:metabolism; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism
D000998:Antiviral Agents; D010940:Plant Proteins; D012313:RNA; D004247:DNA; D009699:N-Glycosyl Hydrolases; D000225:Adenine