Role of the two-component signal transduction and the phosphoenolpyruvate: carbohydrate phosphotransferase systems in competence development of Haemophilus influenzae Rd.
Haemophilus influenzae Rd becomes competent for transformation by nutritional downshift or transient anaerobic growth through a process that requires cyclic AMP receptor protein and adenylate cyclase. Insertion mutations in crr or ptsI of the phosphoenolpyruvate:carbohydrate phosphotransferase system lowered transformation frequencies, and the effect was reversed by the addition of cyclic AMP. However, insertions into H. influenzae homologs of two-component signal transduction genes had no effect on competence.
Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA. mgwinn@welchlink.welch.jhu.edu
M L
ML
Gwinn
D
D
Yi
H O
HO
Smith
J F
JF
Tomb
D006193:Haemophilus influenzae / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014170:Transformation, Genetic
D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase