Detection of radionuclides in capillary electrophoresis using a phosphor-imaging detector.
A capillary electrophoresis (CE) postcolumn radionuclide detector has been developed that uses a commercial phosphor-imaging detector and has been optimized for low-energy beta emitters. Eluant from the separation capillary is deposited on a membrane. Emission from radioactive analytes on the membrane is integrated using the phosphor-imaging system for 10-72 h. Results from the phosphor-imaging system are converted to conventional electropherograms. Modifications to a prior postcolumn CE deposition system have been accomplished by adding a buffer makeup capillary; this increases the electrolyte pH range and improves reproducibility. The limit of detection (LOD) for 35S-labeled analytes is 0.13 amol (8.7 pM or 0.007 Bq), while the LOD for 32P-labeled analytes is 4.9 zmol (0.33 pM or 0.002 Bq), with a linear range for 35S-Met from 1.5 amol to 1.5 fmol.
Department of Chemistry, University of Illinois, Urbana 61801, USA.
S E
SE
Tracht
L
L
Cruz
C M
CM
Stobba-Wiley
J V
JV
Sweedler
D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D019075:Electrophoresis, Capillary; D017952:Ganglia, Invertebrate / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D009474:Neurons / Q000737:chemistry; D011868:Radioisotopes / Q000032:analysis*
D011868:Radioisotopes