Crystal structure of Aplysia ADP ribosyl cyclase, a homologue of the bifunctional ectozyme CD38.
ADP ribosyl cyclase synthesizes the novel secondary messenger cyclic ADP ribose (cADPR) utilizing NAD as a substrate. The enzyme shares extensive sequence similarity with two lymphocyte antigens, CD38 and BST-1, which hydrolyse as well as synthesize cADPR. The crystal structure provides a model for these cell surface enzymes. Cyclase contains two spatially separated pockets composed of sequence conserved residues, suggesting that the cyclization reaction may entail use of distinct sites. The enzyme dimer encloses a cavity which may entrap the intermediate, ADP ribose.
Prasad
G S
GS
Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA.
McRee
D E
DE
Stura
E A
EA
Levitt
D G
DG
Lee
H C
HC
Stout
C D
CD
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000737:chemistry*; D001048:Aplysia / Q000201:enzymology*; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D000943:Antigens, Differentiation; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D000246:Adenosine Diphosphate Ribose; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2; D051997:ADP-ribosyl Cyclase 1