The AngR protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in Vibrio anguillarum.
Vibrio anguillarum virulence is associated with the presence of a plasmid-mediated iron-uptake system expressed under iron-limiting conditions, which consists of the siderophore anguibactin and specific iron-transport proteins. This system is maximally expressed under iron-limiting conditions and requires the AngR protein that acts as a positive regulator of anguibactin biosynthesis and also possess an EntE-like enzymatic function that may play a role in anguibactin biosynthesis. In this work, we demonstrate that in addition to possessing these functions related to anguibactin production, AngR also positively regulated transcription of the iron-transport genes fatA and fatB. We also show that transcription of angR is repressed by Fur under iron-rich conditions. In addition, we present evidence that anguibactin itself enhanced transcription of the iron-transport genes fatA and fatB, independently of AngR and the trans-acting factor (TAF) product(s). The presence of either AngR (together with the TAF product(s)) or anguibactin alone led to a partial level of expression of the iron-transport genes fatA and fatB, while full expression is achieved when AngR, the TAF products and anguibactin are all present.
Chen
Q
Q
Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health Sciences University, Portland 97201-3098, USA.
Wertheimer
A M
AM
Tolmasky
M E
ME
Crosa
J H
JH
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins; D010455:Peptides; D012097:Repressor Proteins / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C071991:FatB protein, Vibrio anguillarum; D026901:Membrane Transport Proteins; D010455:Peptides; D012097:Repressor Proteins; D017262:Siderophores; D014157:Transcription Factors; C063474:angR protein, Vibrio anguillarum; C068477:ferric uptake regulating proteins, bacterial; C071990:FatA protein, bacteria; C049469:anguibactin; D007501:Iron