Deletion of exon b3 of the BCR gene in CML: easy breakpoint mapping by a two-round PCR.
We have performed the molecular analysis for the detection of the BCR-ABL and ABL-BCR fusion genes in 50 patients with myeloproliferative disorders. All patients diagnosed with CML (13 out of 50) were positive for the BCR-ABL hybrid. Six CML patients (46%) showed ABL-BCR amplifications of the Ib-BCR type. All rearrangements but one were concordant. The aberrant case presented a deletion of exon b3, in addition to the alternative Ib-BCR and Ia-BCR. Its possible origin and relevance are briefly discussed.
Cigudosa
J C
JC
Service of Genetics, Faculty of Medicine, University of La Laguna, Tenerife, Spain.
Acosta Almeida
M T
MT
Carrasco Juan
J L
JL
Otero Gómez
A
A
Hernández Nieto
L
L
García Talavera
J
J
García Miranda
J L
JL
D005091:Exons / Q000235:genetics*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D011446:Prospective Studies
D016044:Fusion Proteins, bcr-abl