Multilocational hepatocyte transplantation for treatment of congenital ascorbic acid deficiency rats.
We attempted multilocational hepatocyte transplantation (HCTx) including hepatocyte-bearing polyurethane foam (PUF) to treat congenitally ascorbic acid (AsA) biosynthetic enzyme-deficient (ODS-od/od) rats. Hepatocytes isolated from the liver of congeneic rats were transplanted into the portal vein (Pv), spleen (Sp), omentum (Om), and mesentery (Ms). Hepatocyte-bearing PUF was transplanted into the Om and Ms. Experimental groups were divided into four groups (group I; Pv + Sp, group II; Pv + Sp + Om + Ms, group III; Pv + Sp + hepatocyte-bearing PUF, group IV; control). The average serum AsA level of the surviving rats in group II and III was significantly higher than that in group I 3 mo after HCTx. Histological examination showed small foci of surviving hepatocytes in the Om and Ms tissues and in the connective tissue in the PUF. ODS-od/od rats survived for a long time by multilocational HCTx.
Nakazawa
F
F
Second Department of Surgery, Asahikawa Medical College, Japan.
Onodera
K
K
Kato
K
K
Sawa
M
M
Kino
Y
Y
Imai
M
M
Kasai
S
S
Mito
M
M
Matsushita
T
T
Funatsu
K
K
D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000151:congenital / Q000401:mortality / Q000628:therapy*; D017690:Cell Transplantation / Q000379:methods*; D018914:Cells, Immobilized; D006498:Hepatectomy; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D008643:Mesentery / Q000473:pathology; D009852:Omentum / Q000473:pathology; D011140:Polyurethanes; D051381:Rats; D011922:Rats, Mutant Strains; D016019:Survival Analysis
D011140:Polyurethanes; C028279:polyurethane foam