Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4.
A simple two-step procedure was developed to obtain pure enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Chemical and genetic characterization revealed that the primary structure of enterocin 4 is identical to that of peptide antibiotic AS-48 from Enterococcus faecalis S-48. In contrast to the reported inhibitory spectrum of AS-48, enterocin 4 displayed no activity against gram-negative bacteria.
Departamento de Tecnología de Alimentos, CIT-INIA, Madrid, Spain. han.joosten@chlsnr.nestrd.ch
H M
HM
Joosten
M
M
Nunez
B
B
Devreese
J
J
Van Beeumen
J D
JD
Marugg
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D013293:Enterococcus faecalis / Q000737:chemistry* / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007778:Lactobacillus / Q000187:drug effects; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D001952:Bridged-Ring Compounds; D017931:DNA Primers; C012306:enterocin