Acidic fibroblast growth factor enhances neurite outgrowth and stimulates expression of GAP-43 and T alpha 1 alpha-tubulin in cultured neurones from adult rat dorsal root ganglia.
The affects of acidic fibroblast growth factor (FGF-1) on neurite outgrowth were investigated and related to the levels of mRNA encoding the growth associated protein genes GAP-43 and the neuron specific alpha-tubulin, T alpha 1, in primary cultures of dorsal root ganglia (DRG) neurones from adult rats. In response to FGF-1, sensory neurones produced a high level of neurite outgrowth which was highest (2.70 +/- 0.09-fold over control) at 50 ng/ml of FGF-1. Treatment with 250 ng/ml of a monoclonal anti-nerve growth factor (NGF) antibody, which can block the similar increase in neurite outgrowth produced by 0.1 ng/ml of NGF, did not antagonise the response to 50 ng/ml of FGF-1, showing that FGF-1 has effects on regeneration in adult sensory neurones not mediated via NGF. The estimated ED50 value for FGF-1 was 20 ng/ml for induction of neurite outgrowth. Treatment with FGF-1 elevated the level of mRNA for GAP-43 to 2.9-fold at 50 ng/ml and T alpha 1 alpha-tubulin by 9.2-fold at 10 ng/m, though the FGF-1 concentration/effect range for stimulation of these two mRNA was different from that for neurite outgrowth.
Department of Pharmacology, St. Bartholomew's School of Medicine and Dentistry, London, UK.
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Mohiuddin
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Fernyhough
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Tomlinson
D000367:Age Factors; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D015152:Blotting, Northern; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D019922:GAP-43 Protein; D005727:Ganglia, Spinal / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016900:Neurofilament Proteins / Q000235:genetics; D009475:Neurons, Afferent / Q000187:drug effects / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000235:genetics*
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D014404:Tubulin; D016220:Fibroblast Growth Factor 1