Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection.
A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
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Mweene
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Ito
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Okazaki
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Ono
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Shimizu
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Kida
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000175:diagnosis*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits
D000914:Antibodies, Viral; D000956:Antigens, Viral