Nerve function and blood flow in Otsuka Long-Evans Tokushima Fatty rats with sucrose feeding: effect of an anticoagulant.
To investigate the pathogenesis of diabetic neuropathy in non-insulin-dependent diabetes mellitus, Otsuka Long-Evans Tokushima Fatty rats, an animal model of non-insulin-dependent diabetes mellitus, and non-diabetic Long-Evans Tokushima Otsuka rats were fed with or without sucrose and/or cilostazol, an anticoagulant, for 8 weeks. Sucrose-fed diabetic rats showed a delayed motor nerve conduction velocity, decreased R-R interval variability of electrocardiogram, reduced sciatic nerve blood flow, increased platelet aggregability and a decreased erythrocyte 2,3-diphosphoglycerate concentration compared with non-sucrose-fed diabetic rats and non-diabetic rats. These abnormalities were significantly prevented by treatment with cilostazol without changes in the nerve tissue levels of polyols. These findings indicate that sucrose-fed Otsuka Long-Evans Tokushima Fatty rats may be a useful animal model of neuropathy in non-insulin-dependent diabetes mellitus, and that cilostazol may prevent the development of diabetic neuropathy by modifying vascular factors.
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
N
N
Hotta
N
N
Koh
F
F
Sakakibara
J
J
Nakamura
Y
Y
Hamada
T
T
Hara
K
K
Mori
K
K
Naruse
H
H
Fukasawa
H
H
Kakuta
N
N
Sakamoto
D019794:2,3-Diphosphoglycerate; D000438:Alcohols / Q000378:metabolism; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D050260:Carbohydrate Metabolism; D000077407:Cilostazol; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004195:Disease Models, Animal; D004562:Electrocardiography; D004912:Erythrocytes / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D012584:Sciatic Nerve / Q000098:blood supply* / Q000503:physiopathology; D013395:Sucrose / Q000494:pharmacology*; D013777:Tetrazoles / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood
D000438:Alcohols; D000925:Anticoagulants; D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D007328:Insulin; D013777:Tetrazoles; D014280:Triglycerides; D019794:2,3-Diphosphoglycerate; D013395:Sucrose; D000077407:Cilostazol