Effect of peroxynitrite on erythrocytes.
The action of peroxynitrite on human erythrocytes and erythrocyte membranes was studied. Peroxynitrite (0.1-2 mM) induced a transient decrease of intracellular reduced glutathione, oxidized membrane protein -SH groups, initiated membrane lipid peroxidation and inactivated erythrocyte membrane acetylcholinesterase and ATPase activities. Membranes exposed to peroxynitrite showed aggregation and nitration of proteins and changes in protein organization detectable with a maleimide spin label.
Department of Molecular Biophysics, University of Lódź, Poland. sosmirek@krysia.uni.lodz.pl
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Soszyński
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Bartosz
D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000328:Adult; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003497:Cyclic N-Oxides; D003676:Deferoxamine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008301:Maleimides; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009566:Nitrates / Q000138:chemical synthesis / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013113:Spin Labels; D013438:Sulfhydryl Compounds / Q000378:metabolism
D002800:Cholinesterase Inhibitors; D003497:Cyclic N-Oxides; D004791:Enzyme Inhibitors; D006454:Hemoglobins; D008301:Maleimides; D008563:Membrane Lipids; D008565:Membrane Proteins; D009566:Nitrates; D013113:Spin Labels; D013438:Sulfhydryl Compounds; C043592:maleimide; C039642:peroxynitric acid; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases; D005978:Glutathione; D003676:Deferoxamine; C003959:TEMPO