A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant.
An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.
Eijkman-Winkler Institute for Microbiology, Utrecht University Hospital, The Netherlands.
E M
EM
Mascini
M A
MA
Hazenberg
L A
LA
Verhage
S E
SE
Holm
J
J
Verhoef
H
H
van Dijk
D001426:Bacterial Proteins; D001431:Bacteriological Techniques; D003470:Culture Media / Q000032:analysis; D005098:Exotoxins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification*; D008565:Membrane Proteins; D013297:Streptococcus pyogenes / Q000276:immunology*
D001426:Bacterial Proteins; D003470:Culture Media; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin