Cytotoxicity of two new ribosome-inactivating proteins, cinnamomin and camphorin, to carcinoma cells.
Cinnamomin (two-chain) and camphorin (single-chain), two novel ribosome-inactivating proteins (RIPs) purified from the seeds of Cinnamomum camphora, produced inhibitory effects in cultured carcinoma cells. The IC50 of cinnamomin to the human hepatocarcinoma cell-line 7721 and the melanoma cell-line M21 were 18.8 nmol and 11.7 nmol respectively. The IC50 of camphorin to the human hepatocarcinoma cell-line 7721 was 59 nmol, whereas the melanoma cell-line M21 was not susceptible to camphorin. Furthermore, cinnamomin exhibited a remarkable inhibitory effect on the growth of solid melanoma in the skin of the nude mouse. An R-fragment could be isolated from ribosomes of cinnamomin- or camphorin-treated carcinoma cells after incubation with acidic aniline, indicating that the cytotoxicity of these two new RIPs to carcinoma cells might result from modification to the ribosomes.
Ling
J
J
Shanghai Institute of Biochemistry, Academia Sinica, China.
Liu
W Y
WY
D020418:Algal Proteins; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D004278:DNA, Superhelical / Q000378:metabolism; D004198:Disease Susceptibility; D005656:Fungal Proteins / Q000494:pharmacology*; D006801:Humans; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008819:Mice, Nude; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D011506:Proteins; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes; D014407:Tumor Cells, Cultured
D020418:Algal Proteins; D000970:Antineoplastic Agents; D004278:DNA, Superhelical; D005656:Fungal Proteins; D010940:Plant Proteins; D011506:Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C104406:camphorin protein, Cinnamomum camphora; C061930:cinnamomin, Phytophthora cinnamomi; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins