Halothane alters electrical activity and calcium dynamics in cultured mouse cortical, spinal cord, and dorsal root ganglion neurons.
Halothane inhibits neural plasma membrane Ca(2+)-ATPase, a pump that ejects Ca2+ from the cell after influx through voltage- or ligand-activated channels. Intracellular microelectrode recordings in mouse embryonic cortical and spinal cord neurons showed that halothane and eosin, a pump inhibitor, prolonged repolarization associated with spontaneous bursts of depolarization. These agents also prolonged the repolarization phases of electrically induced action potentials and of capsaicin-mediated Ca(2+)-dependent depolarization in mouse adult dorsal root ganglion neurons. In keeping with these findings, confocal microfluorimetry showed that halothane delayed clearance of intracellular Ca2+ accumulated by N-methyl-D-aspartate stimulation of single neurons.
Wamil
A W
AW
Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN 37232-2125, USA.
Franks
J J
JJ
Janicki
P K
PK
Horn
J L
JL
Franks
W T
WT
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology; D004594:Electrophysiology; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology; D006221:Halothane / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000502:physiology; D051379:Mice; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D013116:Spinal Cord / Q000166:cytology; D013779:Tetrodotoxin / Q000494:pharmacology
D018685:Anesthetics, Inhalation; D005456:Fluorescent Dyes; D013779:Tetrodotoxin; D000252:Calcium-Transporting ATPases; D002211:Capsaicin; D002118:Calcium; D004801:Eosine Yellowish-(YS); D006221:Halothane