Constitutive cytokine release by simian T-cell lymphotrophic virus type I (STLV-I) and human T-cell lymphotrophic virus types I/II (HTLV-I/II) transformed cell lines.
Simian T-cell lymphotrophic virus type I (STLV-I) and human T-cell lymphotrophic virus types I/II (HTLV-I/II) contain the tax gene which can transactivate the transcription of viral and cellular genes including several cytokines. These investigations used two STLV-I and four different HTLV-I/II transformed cell lines to quantitate constitutive cytokine release, p24 antigen production, and to correlate gag p24 antigen and cytokine release. These investigations are the first to report 1) quantitative comparison of constitutive release of multiple cytokines by several STLV-I and HTLV-I/II transformed cell lines and to determine the cytokines constitutively released by STLV-I transformed cell lines as IL-6, b-FGF, and GM-CSF (and TNF-beta, and PDGF in a higher viral producing line); 2) statistically significant differences in levels of cytokines produced by STLV-I and HTLV-I/II transformed cell lines, dependent on the method of results quantification; and 3) a correlation between levels of STLV-I and HTLV-I/II and gag p24 antigen production and cytokine production.
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, USA.
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D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016207:Cytokines / Q000096:biosynthesis*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D015683:Gene Products, gag / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006133:Growth Substances / Q000096:biosynthesis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000502:physiology*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D007700:Kinetics; D008233:Lymphotoxin-alpha / Q000096:biosynthesis; D008251:Macaca; D010215:Papio; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis; D015303:Simian T-lymphotropic virus 1 / Q000276:immunology / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D002135:Calcium-Binding Proteins; D016207:Cytokines; D015683:Gene Products, gag; D006133:Growth Substances; D015850:Interleukin-6; D008233:Lymphotoxin-alpha; D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; C034733:oncomodulin; D016222:Fibroblast Growth Factor 2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor