Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain.
Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.
Date
I
I
Department of Neurological Surgery, Okayama University Medical School, Japan.
Miyoshi
Y
Y
Ono
T
T
Imaoka
T
T
Furuta
T
T
Asari
S
S
Ohmoto
T
T
Iwata
H
H
D000818:Animals; D016380:Brain Tissue Transplantation; D002214:Capsules; D017690:Cell Transplantation / Q000295:instrumentation / Q000379:methods*; D004298:Dopamine / Q000378:metabolism*; D016716:PC12 Cells / Q000637:transplantation*; D011137:Polystyrenes; D051381:Rats; D012117:Resins, Synthetic; D012685:Sepharose; D014183:Transplantation, Heterologous / Q000379:methods
D002214:Capsules; D011137:Polystyrenes; D012117:Resins, Synthetic; C003321:polystyrene sulfonic acid; D012685:Sepharose; D004298:Dopamine