Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.
Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
K
K
Tanaka
T
T
Miyazaki
S
S
Maesaki
K
K
Mitsutake
H
H
Kakeya
Y
Y
Yamamoto
K
K
Yanagihara
M A
MA
Hossain
T
T
Tashiro
S
S
Kohno
D001483:Base Sequence; D003453:Cryptococcosis / Q000175:diagnosis* / Q000382:microbiology*; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005800:Genes, Fungal; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000382:microbiology*; D009172:Mycology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004271:DNA, Fungal