The early gene region completes the nucleotide sequence of Lactobacillus delbrueckii subsp. lactis phage LL-H.
Transcription of genes from phage LL-H can be divided into an early phase and a late phase. The early gene region was located in a 5.9-kb segment of the phage LL-H genome and it was part of the sequence that completed the phage LL-H genome sequence, 34 659 bp in size. Phage LL-H is the first completely sequenced Lactobacillus phage. In the main coding strand of phage LL-H genome 48 putative ORFs could be detected, but only four small putative ORFs could be found in the opposite strand. The ORFs covered 85.6% of the main coding strand. Function could be assigned to eleven of the phage LL-H ORFs either by biochemical analyses or by database homologies. A single-strand-binding protein, SSB, was detected in addition to the previously determined functions (small and large subunits of terminase, intron-encoded endonuclease, six structural proteins, phage lysin). For 15 additional ORFs of phage LL-H homology was detected in databases, but no function could be inferred for them.
Mikkonen
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Department of Biology, University of Oulu, Finland. Merja.Mikkonen@csc.fi
Räisänen
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L
Alatossava
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T
D001435:Bacteriophages / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D017781:Genes, Immediate-Early / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D007778:Lactobacillus / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D014774:Virulence / Q000235:genetics