Time course of luciferyl adenylate synthesis in the firefly luciferase reaction.
The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed. The properties of luciferyl adenylate, enzymatically or chemically synthesized, as substrate of luciferase, have been compared. The potential use of luciferyl adenylate for luciferase detection is here proposed.
Dukhovich
A
A
Departamento de Bioquímica, Instituto de Investigaciones Biomedicas del CSIC, Universidad Autónoma de Madrid, Spain.
Sillero
A
A
Sillero
M A
MA
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
D000255:Adenosine Triphosphate; D008156:Luciferases