Evidence that the Wiskott-Aldrich syndrome protein may be involved in lymphoid cell signaling pathways.
Wiskott-Aldrich syndrome is an X-linked combined immunodeficiency affecting cells of several different hemopoietic lineages. The Wiskott-Aldrich syndrome protein (WASP), which has no homology with any other known protein families, is rich in proline motifs known to contribute to Src homology 3 binding sites. However, its function has not been determined. The Tec family of cytoplasmic tyrosine kinases, which include Btk (the X-linked agammaglobulinemia gene), Itk, and Tec, is thought to be involved in lymphoid cell signaling pathways. In this work, we show binding of WASP to the Src homology 3 domains of Btk, Itk, Tec, Grb2, and phospholipase C-gamma, which suggests a function for WASP in lymphoid cell signaling.
Institute of Child Health, University of London, United Kingdom.
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Cory
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MacCarthy-Morrogh
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Banin
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Gout
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Brickell
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Levinsky
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Kinnon
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Lovering
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000378:metabolism*; D001665:Binding Sites; D002051:Burkitt Lymphoma / Q000473:pathology; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014923:Wiskott-Aldrich Syndrome / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D051316:Wiskott-Aldrich Syndrome Protein; D018909:src Homology Domains / Q000502:physiology*; D019061:src-Family Kinases / Q000737:chemistry / Q000378:metabolism*
D010446:Peptide Fragments; D011506:Proteins; D011993:Recombinant Fusion Proteins; C495767:WAS protein, human; D051316:Wiskott-Aldrich Syndrome Protein; D011392:Proline; D019061:src-Family Kinases