Penetration of airborne microorganisms through a surgical mask and a dust/mist respirator.
This study investigated bacterial penetration of different bacterial shapes, aerodynamic sizes, and flow rates through a surgical mask and a dust/mist respirator. The bacterial penetrations were compared with those of spherical corn oil particles of the same aerodynamic diameter tested under the same conditions. The tests were performed at different levels of aerosol penetration. Bacteria, ranging from spherical to rod-shaped with a high aspect (length to width) ratio, were selected as test agents. Among these, Pseudomonas fluorescens physically simulates Mycobacterium tuberculosis by shape and size. The concentrations of bacteria upstream and downstream of the test devices were measured with an aerodynamic size spectrometer. This instrument was found to measure the total viable and nonviable bacterial concentration effectively and dynamically over the entire bacterial size range down to 0.5 microns in aerodynamic size. The results indicate that the spherical corn oil particles and the spherical Streptococcus salivarius bacteria have the same penetration in the size range from 0.9 to 1.7 microns. It has been found that rod-shaped bacteria penetrate less. The penetration difference between the spherical and rod-shaped bacteria depends on the aspect ratio of the bacteria. For an aspect ratio of 4, the penetration of rod-shaped bacteria is about half that of spherical ones. Thus, it is projected that a respirator with 90% efficiency against spherical microorganisms or test particles (10% penetration) will be 95% efficient against rod-shaped microorganisms of the same aerodynamic equivalent diameter with an aspect ratio of 3 to 4, such as Mycobacterium tuberculosis (5% penetration).
Department of Environmental Health, University of Cincinnati, OH 45267-0056, USA.
K
K
Willeke
Y
Y
Qian
J
J
Donnelly
S
S
Grinshpun
V
V
Ulevicius
D000336:Aerosols; D000391:Air Microbiology; D000395:Air Pollutants, Occupational; D004391:Dust; D004868:Equipment Failure; D005069:Evaluation Studies as Topic; D008397:Masks / Q000592:standards*; D009169:Mycobacterium tuberculosis; D010316:Particle Size; D011551:Pseudomonas fluorescens; D012134:Respiratory Protective Devices / Q000592:standards*
D000336:Aerosols; D000395:Air Pollutants, Occupational; D004391:Dust