Relationship of neuropeptides to nitrergic innervation of the canine laryngeal glands.
The participation of vasoactive intestinal polypeptide (VIP) or calcitonin gene-related peptide (CGRP) in the nitrergic innervation of the canine laryngeal glands was investigated using a double-staining technique of NADPH-diaphorase (NADPHd) histochemistry and VIP or CGRP immunohistochemistry. NADPHd-positive nerve fibers with varicosities appeared to terminate in some acinar cells. Double staining revealed that NADPHd reactivity and VIP- or CGRP-like immunoreactivity were colocalized in some nerve fibers distributed around the acini. A cluster of NADPHd-positive cells were occasionally found in the larynx. Many NADPHd-positive cells had VIP-like immunoreactivity and no NADPHd-positive cells were CGRP-like immunoreactive. These findings suggest that nitric oxide participates in the neural control of the laryngeal exocrine secretion in cooperation with intrinsic VIP and/or extrinsic CGRP.
Department of Otolaryngology, Kyoto Prefectural University of Medicine, Japan.
Y
Y
Hisa
T
T
Uno
N
N
Tadaki
S
S
Koike
H
H
Banba
M
M
Tanaka
H
H
Okamura
Y
Y
Ibata
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D004285:Dogs; D007823:Laryngeal Nerves / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology*; D013194:Staining and Labeling; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
D009569:Nitric Oxide; D014660:Vasoactive Intestinal Peptide; D015740:Calcitonin Gene-Related Peptide