The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames.
We report the sequence of a 20 300 bp DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV. This segment contains 13 complete open reading frames (ORFs) and part of another ORF, altogether covering 84.2% of the entire sequence, five of which correspond to the previously characterized KIN28, MSS2, PHO2, POL3/CDC2 and DUN1 genes. One putative protein, D2358p, shares considerable homology with an O-sialoglycoprotein endopeptidase from Pasteurella haemolytica serotype A1. The putative product of D2325 contains the characteristic consensus motif of triacylglycerol lipases. D2320p and D2352p have a putative 'leucine-zipper' structure and a RNA-binding region Rnp-1 signature, respectively.
Departamento de Microbiologia y Genética, Universidad de Salamanca, Spain.
J E
JE
Saiz
M J
MJ
Buitrago
R
R
Garcia
J L
JL
Revuelta
F
F
Del Rey
D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008049:Lipase / Q000235:genetics; D016978:Mannheimia haemolytica / Q000201:enzymology / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D008049:Lipase; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase