Increased expression of dystrophin, beta-dystroglycan and adhalin in denervated rat muscles.
To evaluate a potential regulatory role of the nerve, the distribution and expression of dystrophin, of beta-dystroglycan (43DAG) and adhalin (50DAG), two of the dystrophin-associated proteins and utrophin (dystrophin related protein or DRP) were studied in rat muscles after 2 weeks of denervation. We found that dystrophin, beta-dystroglycan and adhalin were overexpressed in denervated muscle, whereas utrophin did not increase and was found only in the post-synaptic membrane. The study of the distribution of dystrophin in the sarcolemma of single muscle fibres indicates that the molecular organization of dystrophin was maintained after denervation. Dystrophin in addition of forming a scaffold around the fibre was found around the clusters of AChR that reappeared in the extra-synaptic membrane after denervation. Also beta-dystroglycan colocalises at these clusters. These results suggest that the increase in dystrophin, beta-dystroglycan and adhalin is correlated with the reappearance of AChRs in the extra synaptic membrane.
CNR Unit for Muscle Biology and Physiopathology, Dipartimento di Scienze Biomediche Sperimentali, University of Padova, Italy.
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Biral
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Senter
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Salviati
D000818:Animals; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D049030:Dystroglycans; D016189:Dystrophin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000378:metabolism*; D017474:Receptors, Laminin / Q000378:metabolism*; D049031:Sarcoglycans; D013570:Synaptic Membranes / Q000378:metabolism; D049411:Utrophin
D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011950:Receptors, Cholinergic; D017978:Receptors, Growth Factor; D017474:Receptors, Laminin; D049031:Sarcoglycans; D049411:Utrophin; D049030:Dystroglycans