The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils.
Monoclinic calcium pyrophosphate dihydrate (m-CPPD) crystals were synthesized and characterized using physical methods, IgG binding to m-CPPD crystals was quantitated, and the effect of IgG or plasma opsonization on m-CPPD-induced neutrophil activation was determined. Adsorption of IgG to crystals was measured using fluorescent-labelled FITC-IgG. Neutrophil activation by uncoated m-CPPD and crystals precoated with IgG or plasma was measured using luminol-enhanced chemiluminescence, superoxide anion generation, and myeloperoxidase release. m-CPPD bound small (compared to triclinic CPPD) but significant amounts of IgG and induced a strong activation of neutrophils at low concentrations of crystals. The rate and extent of chemiluminescence, superoxide anion production, and degranulation was not affected by precoating m-CPPD crystals with IgG during the early phase of neutrophil responses, but was inhibited by the precoating of crystals with plasma.
Winternitz
C I
CI
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
Jackson
J K
JK
Burt
H M
HM
D000327:Adsorption; D002131:Calcium Pyrophosphate / Q000097:blood* / Q000737:chemistry* / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D003460:Crystallization; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010316:Particle Size; D011485:Protein Binding; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D014961:X-Ray Diffraction
D007074:Immunoglobulin G; D013481:Superoxides; D002131:Calcium Pyrophosphate