Binding between Pseudomonas aeruginosa adhesins and human salivary, tracheobronchial and nasopharyngeal mucins.
Endogenous aspiration of nasooropharyngeal secretions is a major source of Pseudomonas aeruginosa to lungs. To understand the mechanism of binding between P. aeruginosa and nasooropharyngeal secretions, we have utilized an overlay binding assay employing P. aeruginosa nonpilus protein adhesins (18 kDa and 15 kDa) and mucins from human saliva, tracheobronchial and nasopharyngeal secretions. Nasopharyngeal mucin bound to both the 18 kDa and 15 kDa adhesins. A high molecular weight salivary mucin and tracheobronchial mucin bound only to the 18 kDa adhesin. On the other hand, asialonasopharyngeal and asialotracheobronchial mucins did not bind to either of the adhesins. Binding of nasopharyngeal mucin to the 18 kDa and 15 kDa was not inhibitable by glycoproteins, bovine submaxillary mucins and sialic acid. Further, a low molecular weight salivary mucin which contains sialylated oligosaccharides also did not bind to either of the adhesins. Collectively, the results suggest that binding between P. aeruginosa and mucins may involve specific receptors and adhesins.
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214, USA.
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Reddy
D018829:Adhesins, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001980:Bronchi / Q000378:metabolism; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism*; D009305:Nasopharynx / Q000378:metabolism; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012463:Saliva / Q000378:metabolism; D012794:Sialic Acids; D014132:Trachea / Q000378:metabolism
D018829:Adhesins, Bacterial; D009077:Mucins; D012794:Sialic Acids