Expression of the gene for tear lipocalin/von Ebner's gland protein in human prostate.
Northern analysis of human multiple tissue blots containing poly A+ RNA from spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes revealed that a prostate specific transcript hybridizes to a tear lipocalin/von Ebner's gland protein (TL/VEGP) gene probe. To characterize this transcript, the corresponding cDNA was amplified by reverse transcription (RT)-PCR. Cloning and sequence analysis showed that it was identical to the tear lipocalin cDNA isolated from human lachrymal glands. Immunohistochemical analysis on thin layer sections of human prostate using a tear lipocalin specific antiserum confirmed the expression of this cDNA in prostate. Thus, our results clearly argue against a unique function of TL/VEGP in human tear fluid or saliva. The human cDNA was expressed in E. coli using the pQE system yielding a recombinant protein which shows biochemical properties identical to the native TL/VEGP.
Holzfeind
P
P
Institut für Mikrobiologie (Med. Fakultät), Universitat Innsbruck, Austria.
Merschak
P
P
Rogatsch
H
H
Culig
Z
Z
Feichtinger
H
H
Klocker
H
H
Redl
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D054835:Lipocalin 1; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis* / Q000235:genetics; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014158:Transcription, Genetic
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; C518443:LCN1 protein, human; D054835:Lipocalin 1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides