Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies.
A panel of 18 monoclonal antibodies (mAbs) defined by the third workshop as specific for platelets, clustered in three preliminary groups: PC7, PC13 and PC27. These mAbs were further analysed by immunoprecipitation using extracts of iodinated and biotinylated peripheral blood mononucleated cells (PBMC) and platelets. We could confirm the existence of mAbs with specificities to WC9 (in PC7) and CD41/61 (in PC13). Two mAbs formed a new cluster, WC13, which may be homologous to human CD31 (in PC27). The influence of EDTA and thrombin on the expression of the different antigens on the platelet membrane was assessed by flow cytometry (FCM) analysis, as well as cross-reactivity with platelets from different species.
Facultad de Veterinaria, Universidad de Cordoba, Dpto de Genetica, Spain.
A
A
Mateo
J
J
Perez de la Lastra
A
A
Moreno
R
R
Dusinsky
F
F
Bilka
M
M
Simon
L
L
Horovska
D
D
Llanes
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000145:classification / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions / Q000276:immunology; D005434:Flow Cytometry; D039661:Integrin beta3; D016023:Integrins / Q000276:immunology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039661:Integrin beta3; D016023:Integrins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins