Functional conservation of mouse Notch receptor family members.
All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr virus nuclear antigen 2. Furthermore the intracellular region of int3 was shown to transactivate the Epstein Barr virus TP1 promoter. These results indicate that all mouse Notch family members have biochemical functions similar to mNotch1, which transduces proliferative signal by direct interaction with the DNA binding protein RBP-Jkappa.
Department of Medical Chemistry, Kyoto University, Faculty of Medicine, Sakyo-ku, Japan.
H
H
Kato
T
T
Sakai
K
K
Tamura
S
S
Minoguchi
Y
Y
Shirayoshi
Y
Y
Hamada
Y
Y
Tsujimoto
T
T
Honjo
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D019309:Epstein-Barr Virus Nuclear Antigens / Q000737:chemistry / Q000378:metabolism; D005982:Glutathione Transferase; D004854:Herpesvirus 4, Human / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D019309:Epstein-Barr Virus Nuclear Antigens; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase