Hydrosalpinx fluid has embryotoxic effects on murine embryogenesis: a case for prophylactic salpingectomy.
OBJECTIVE
To ascertain if hydrosalpinges are associated with reduced pregnancy rates and increased pregnancy loss after IVF-ET. Increased volume and leakage of hydrosalpinx fluid may exert negative effects on follicular development and embryo quality and/or render the uterine environment hostile to embryogenesis. We undertook this study to examine the effect of hydrosalpinx fluid on murine embryogenesis in vitro.


METHODS
Descriptive study.


METHODS
Tertiary care facility.


METHODS
Premenopausal females undergoing salpingectomy or salpingostomy for hydrosalpinges.


METHODS
Collection of discarded hydrosalpinx fluid and development of a dose response curve for the effect of hydrosalpinx fluid on murine embryogenesis.


METHODS
Development of single cell mouse embryos in vitro.


RESULTS
All samples of tubal fluid obtained from hydrosalpinges demonstrated a significant embryo toxic effect at either the 100% or 10% concentration. Hydrosalpinx fluid demonstrated pH values (8.45 to 8.65) significantly higher than the physiologic range. Correction of pH to that of media did not affect cavitation rate.


CONCLUSIONS
There is a well-defined and significant toxic effect of hydrosalpinx fluid. Procedures such as salpingectomy or proximal tubal occlusion to circumvent the passage of hydrosalpinx fluid into the uterine cavity may have beneficial effects on the developmental environment for embryos in vivo.
Mukherjee
T
T
Department of Obstetrics and Gynecology, Mount Sinai Medical Center, New York, New York 10029, USA.
Copperman
A B
AB
McCaffrey
C
C
Cook
C A
CA
Bustillo
M
M
Obasaju
M F
MF
D000818:Animals; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D005122:Exudates and Transudates; D005184:Fallopian Tube Diseases / Q000503:physiopathology* / Q000601:surgery*; D005187:Fallopian Tubes / Q000601:surgery*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008813:Mice, Inbred ICR; D009994:Osmolar Concentration; D011247:Pregnancy; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens