The inhibitory effect of cyclophosphamide on camel retina acetylcholinesterase activity.
Kinetic parameters for the effect of cyclophosphamide (CP) on the camel retina acetylcholinesterase (AChE) activity were investigated for the first time in the present study. It was found that 18 micrograms of retina protein and an incubation time of 4.0 min were suitable conditions for linear of AChE activity. The CP effect was independent of time of incubation with AChE before the addition of substrate, which shows it reversible action. Moreover, dilution data prove that CP is a reversible inhibitor of camel retina AChE. Cyclophosphamide (0.2-2.4 mM) inhibited activity of camel retina in a concentration-dependent fashion, the IC50 being about 1.17 mM. The Michaelis constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.106 mM and the Vmax was 0.765 mumol/min/mg protein. Dixon as well as Lineweaver-Burk plots and their secondary replots indicated that the nature of the inhibition was of the pure noncompetitive type. The value of Ki was estimated as 0.763 mM by the primary Dixon and secondary replots of the Lineweaver-Burk plot.
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
F
F
al-Khwyter
M A
MA
Kamal
A A
AA
al-Jafari
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D002162:Camelus; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004795:Enzyme Stability / Q000187:drug effects; D007700:Kinetics; D007928:Lethal Dose 50; D016014:Linear Models; D012160:Retina / Q000187:drug effects* / Q000201:enzymology
D018906:Antineoplastic Agents, Alkylating; D002800:Cholinesterase Inhibitors; D003520:Cyclophosphamide