Biotin-labeled antigen: a novel approach for detection of Puumala virus-specific IgM.
A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on baculovirus-expressed Puumala (PUU) virus nucleocapsid protein, was developed for rapid diagnosis of nephropathia epidemica. The recombinant antigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated to biotin. The biotin-streptavidin system, in combination with the mu-capture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated using a panel of 160 patient and control sera.
Ivanov
A
A
Chumakov Institute of Poliomyelitis and Viral Encephalitides, Ac. Med. Sci., Moscow, Russia.
Vapalahti
O
O
Lankinen
H
H
Tkachenko
E
E
Vaheri
A
A
Niklasson
B
B
Lundkvist
A
A
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D001710:Biotin; D002213:Capsid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000175:diagnosis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D001710:Biotin