DNA-damaging agents induce the 72-kD heat shock protein in SV40 transformed normal human fibroblasts.
In order to elucidate the involvement of DNA damage in the induction of heat shock proteins (stress proteins), we examined the induction of 72-kD heat shock protein (HSP72) in an SV40-transformed human fibroblast cell line (WI38VA13) which was exposed to various DNA-damaging agents, including 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3 -nitro-methylmethane sulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, and 4-nitroquinoline-N-oxide. Induction of HSP72 was detected by the indirect immunofluorescence method using a monoclonal antibody. All the DNA-damaging agents used in this study induced HSP72 on human fibroblasts. This result indicates that DNA damage is one trigger for the induction of HSP72.
Department of Dermatology, Nara Medical University, Japan.
T
T
Muramatsu
H
H
Ohno
T
T
Shirai
A
A
Takahashi
T
T
Ohnishi
D000970:Antineoplastic Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002461:Cell Line, Transformed; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D019084:Fluorescent Antibody Technique, Indirect; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000187:drug effects*; D006801:Humans
D000970:Antineoplastic Agents; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins