Developmental changes in transcription factors associated with the nuclear matrix of chicken erythrocytes.
The nuclear matrix has roles in organizing nuclear DNA and in controlling transcription. Transcription factors are associated with the nuclear matrix, with the spectra of transcription factors differing from one cell type to another. In this study we identified the transcription factors and enzymes functioning in the regulation of gene expression that were associated with nuclear matrix and nonmatrix nuclear fractions in erythrocytes isolated from chick embryos at different stages of development, anemic and normal adult birds. We found that the primitive erythroid nuclear matrix had the greatest histone deacetylase activity and highest levels of several transcription factors, including GATA-1, CACCC-binding proteins, and NF1. These transcription factors have key roles in erythroid-specific gene expression. The levels of these transcription factors were lower in the nonmatrix and matrix fractions isolated from definitive erythrocytes. For primitive and definitive erythrocytes, the level of CACCC-binding proteins in the nuclear matrix fraction was greater than that of Sp1. The relative levels of these transcription factors were reversed in the nonmatrix fraction. Casein kinase II was not found in erythroid nuclear matrices. The observed erythroid lineage specific alterations in erythroid nuclear matrix transcription factor composition and abundance may be involved in erythroid-specific gene expression.
Sun
J M
JM
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Chen
H Y
HY
Litchfield
D W
DW
Davie
J R
JR
D000818:Animals; D001483:Base Sequence; D047390:Casein Kinase II; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology*; D050981:Erythroid-Specific DNA-Binding Factors; D005914:Globins / Q000235:genetics; D006655:Histone Deacetylases / Q000378:metabolism; D008969:Molecular Sequence Data; D025542:Neurofibromin 1; D015530:Nuclear Matrix / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D025542:Neurofibromin 1; D011506:Proteins; D014157:Transcription Factors; D005914:Globins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D006655:Histone Deacetylases