A pure, monolayer culture of Merkel cells from sinus hair follicles of the rat.
Merkel cells, mechanoreceptor cells in the skin, were dissociated from sinus hair follicles of the whisker pads of newborn rats, and cultured in a serum-free medium. Based on the uptake of quinacrine, a specific fluorescence marking dye, more than 90% of the cells in culture were identified as Merkel cells. During monolayer culture on a poly-L-lysine-coated coverglass surface, Merkel cells exhibited a flat round morphology and often extended thin lamellae on their fringe. Small particles eliciting quinacrine fluorescence were present throughout these cells, with the exception of the lamellae or nuclei. Most Merkel cells died in 48-72 h in the serum-free medium. Fetal calf serum added to the medium protected Merkel cells from the cell death, and the Merkel cells had a fibroblast-like morphology in the serum-containing medium.
Department of Physiology, National Defense Medical School, Saitama, Japan. jfukuda@aqu.bekkoame.or.jp
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D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death; D002478:Cells, Cultured / Q000166:cytology; D017364:Collagenases; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D018859:Hair Follicle / Q000166:cytology*; D018862:Merkel Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D011796:Quinacrine; D051381:Rats; D014738:Vibrissae / Q000166:cytology
D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D017364:Collagenases; D011796:Quinacrine