Fluorescence-detected polymerization kinetics of human alpha 1-antitrypsin.
The time dependence of the human alpha 1-antitrypsin polymerization process was studied by means of the intrinsic fluorescence stopped-flow technique as well as the fluorescence-quenching-resolved spectra (FQRS) method and native PAGE. The polymerization was induced by mild denaturing conditions (1 M GuHCl) and temperature. The data show that the dimer formation reaction under mild conditions was followed by an increase of fluorescence intensity. This phenomenon is highly temperature sensitive. The structure of alpha 1-antitrypsin dimer resembles the conformation of antithrombin III dimer. In the presence of the denaturant the polymerization process is mainly limited to the dimer state. The alpha 1-antitrypsin activity measurements confirm monomer-to-dimer transition under these conditions. These results are in contrast to the polymerization process induced by temperature, where the dimer state is an intermediate step leading to long-chain polymers. On the basis of stopped-flow and electrophoretic data it is suggested that both C-sheet as well as A-sheet mechanisms contribute to the polymerization process under mild conditions.
Koloczek
H
H
University of Agriculture, Department of Biochemistry, Kraków, Poland. koloczek@mol.uj.edu.pl
Guz
A
A
Kaszycki
P
P
D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D007700:Kinetics; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013696:Temperature; D000515:alpha 1-Antitrypsin / Q000737:chemistry* / Q000494:pharmacology
D006146:Guanidines; D011108:Polymers; D000515:alpha 1-Antitrypsin; D019791:Guanidine