Developmental changes in rabbit juxtamedullary proximal convoluted tubule water permeability.
The mammalian proximal tubule reabsorbs the bulk of the glomerular filtrate in a nearly isosmotic fashion due to the high osmotic water permeability (Pf) of this segment. Although the characteristics of proximal tubule water transport have been studied in the adult proximal tubule, little is known about the neonatal segment. The present study directly measured the Pf and diffusional water permeability (PDW) of neonatal (10 +/- 2 day old) and adult rabbit juxtamedullary proximal convoluted tubules (PCT) using in vitro microperfusion. The Pf of neonatal juxtamedullary PCT was greater than the Pf of adult juxtamedullary PCT. In contrast, the PDW was not different between the two groups. The Pf and PDW values of both neonatal and adult tubules were inhibited to the same degree by p-chloromercuribenzene sulfonate and had identical activation energies. The transepithelial reflection coefficients of NaCl and NaHCO3 were also found to be similar in both the neonatal and adult proximal tubules. Thus neonatal and adult juxtamedullary PCT have many characteristics of water transport that are identical; however, neonatal Pf is three to five times that of the adult value. This difference in Pf with identical PDW values may give an insight into the transepithelial pathway for water movement in the neonatal tubule.
Quigley
R
R
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235-9063, USA.
Baum
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D004058:Diffusion; D007679:Kidney Medulla; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D009995:Osmosis; D010539:Permeability; D011817:Rabbits; D017693:Sodium Bicarbonate / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D014867:Water / Q000378:metabolism*
D014867:Water; D012965:Sodium Chloride; D017693:Sodium Bicarbonate