Platelet plasma membrane in subjects with primary nocturnal enuresis: effect of desmopressin.
Plasma membrane fluidity of platelets (PLT) obtained from subjects with primary nocturnal enuresis (PNE) and healthy controls was investigated before and after addition of desmopressin (DDAVP). Membrane fluidity was studied by measuring steady-state fluorescence anisotropy of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3, 5-hexatriene incorporated into PLT plasma membrane. Our results show an increase in membrane fluidity at the surface level of PLT from subjects with PNE. Moreover, the addition of DDAVP induces a stable and significant decrease of membrane fluidity in both groups. These results suggest alterations of the lipid order in the exterior part of the PLT plasma membrane from patients with PNE.
Department of Pediatrics, University of Ancona, Italy.
P L
PL
Giorgi
R
R
Fiorini
M
M
Biraghi
A
A
Kantar
D000293:Adolescent; D000328:Adult; D016880:Anisotropy; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002648:Child; D002784:Cholesterol / Q000097:blood; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004775:Enuresis / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects / Q000502:physiology; D010743:Phospholipids / Q000097:blood; D012076:Renal Agents / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
D005456:Fluorescent Dyes; D010743:Phospholipids; D012076:Renal Agents; D014280:Triglycerides; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D002784:Cholesterol; D003894:Deamino Arginine Vasopressin