Hemorrhagic transformation and microvascular integrity during focal cerebral ischemia/reperfusion.
Hemorrhagic transformation after cerebral ischemia is a well known clinical concern. The frequency of intact basal lamina (BL), identified by laminin antigen, in hemorrhagic and nonhemorrhagic zones after middle cerebral artery occlusion (MCA:O) and 3-h MCA:O with reperfusion in adolescent male baboons was assessed. Parenchymal hemoglobin was not detected prior to 24-h reperfusion. A significant decrease in the density of laminin (BL) in hemorrhagic zones (6.2 +/- 2.4) compared with nonhemorrhagic ischemic zones (10.5 +/- 2.4) (p < 0.05) and nonischemic basal ganglia (17.0 +/- 2.7) (p < 0.01) was observed. Time-dependent changes in BL integrity appear linked to the extravasation of blood components.
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.
G F
GF
Hamann
Y
Y
Okada
G J
GJ
del Zoppo
D000818:Animals; D001485:Basement Membrane / Q000503:physiopathology; D002545:Brain Ischemia / Q000503:physiopathology*; D002543:Cerebral Hemorrhage / Q000503:physiopathology*; D007797:Laminin / Q000032:analysis; D008297:Male; D008833:Microcirculation / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D010215:Papio; D015427:Reperfusion Injury / Q000503:physiopathology*
D007797:Laminin