Decreased expression of HSP47 in highly malignant mouse fibrosarcoma.
HSP47, a heat shock-inducible glycoprotein of M(r) = 47,000, is assumed to be a molecular chaperone specific for collagen. In addition to its stress inducibility, HSP47 has transformation-sensitivity; that is, the synthesis of HSP47 decreases after malignant transformation. The expression of several HSPs in mouse fibrosarcoma, QRsP-1 and QRsP-4 were examined. HSP47 expression was weaker in the highly malignant QRsP-4 than in the weakly malignant QRsP-1, whilst HSC70 expression was similar in both cell lines. These findings suggested an important role of HSP47 as a marker of tumour malignancy of QRsP cell lines.
Shirakami
T
T
Senior Research Fellow Laboratory, Kureha Chemical Industry, Co., Ltd., Tokyo, Japan.
Tsuzuki
T
T
Morino
M
M
Kiyosuke
Y I
YI
Yoshimura
M
M
Yoshikumi
C
C
Okada
F
F
Hosokawa
M
M
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D005354:Fibrosarcoma / Q000737:chemistry / Q000378:metabolism*; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D051379:Mice; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000737:chemistry; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012334:RNA, Neoplasm; C494661:Serpinh1 protein, mouse