Asm-1+, a Neurospora crassa gene related to transcriptional regulators of fungal development.
This report describes the identification, cloning, and molecular analysis of Asm-1+ (Ascospore maturation 1), the Neurospora crassa homologue of the Aspergillus nidulans stuA (stunted A) gene. The Asm-1+ gene is constitutively transcribed and encodes an abundant, nucleus-localized 68.5-kD protein. The protein product of Asm-1+ (ASM-1), contains a potential DNA-binding motif present in related proteins from A. nidulans (StuA), Candida albicans (EFGTF-1), and Saccharomyces cerevisiae (Phd1 and Sok2). This motif is related to the DNA binding motif of the Swi4/Mbp1/Res family of transcription factors that control the cell cycle. Deletion of Asm-1+ destroys the ability to make protoperithecia (female organs), but does not affect male-specific functions. We propose that the APSES domain (ASM-1, Phd1, StuA, EFGTF-1, and Sok2) defines a group of proteins that constitute a family of related transcription factors involved in the control of fungal development.
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA.
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Aramayo
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Peleg
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Addison
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Metzenberg
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
C104110:ASM-1 protein, Neurospora crassa; D004271:DNA, Fungal; D005656:Fungal Proteins; D014157:Transcription Factors