Lack of lecithin: retinol acyltransferase activity in chick lungs.
Our previous study revealed that no retinyl esters were detectable in chick and hen lungs, suggesting that the retinol esterification system may be absent in these tissues. This possibility encouraged us to investigate whether chick lungs exhibit the activity of a retinol esterifying enzyme, i.e., lecithin: retinol acyltransferase (LRAT). The LRAT activity was assayed with dilauroyl phosphatidylcholine and either complex of retinol-cellular retinol-binding protein, type two or retinol-cellular retinol-binding protein in microsomal preparations of lung, duodenum and liver of 7-day-old chicks. Relatively high levels of LRAT activity were present in the duodenum and the liver of chicks as well as in the rat lung. However, the chick lung exhibited no LRAT activity. The lungs of both rat and chick showed similar and low levels of acyl-CoA: retinol acyltransferase (ARAT) activity, but only rat lung, but not chick lung, contained a detectable amount of retinyl esters. Thus, the retinyl ester storage in the lung seems to depend on the presence of LRAT activity in the lung, but it is independent of the presence of ARAT activity in the lung. The absence of LRAT activity and retinyl esters in the chick lung suggests that the retinol in the chick lung may not be provided from retinyl ester storage, and the retinol transferred directly from serum should be utilized to generate retinoic acid.
Department of Nutrition, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
S
S
Takase
Y
Y
Matsumoto
T
T
Goda
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004386:Duodenum / Q000201:enzymology; D004951:Esterification; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D012177:Retinol-Binding Proteins / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular; D013045:Species Specificity; D014801:Vitamin A / Q000378:metabolism
C518458:Rbp1 protein, rat; C518461:Rbp2 protein, rat; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A; D000217:Acyltransferases; C056942:lecithin-retinol acyltransferase