Typing of X chromosomes bearing Tabby allele in mouse preimplantation embryos by detection of a microsatellite marker.
Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.
Department of Reproductive and Developmental Biology, University of Tokyo, Japan.
R P
RP
Valdivia
T
T
Kunieda
M
M
Hattori
Y
Y
Toyoda
D000818:Animals; D001755:Blastocyst; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D011247:Pregnancy; D014960:X Chromosome / Q000235:genetics*
D005819:Genetic Markers