Sequencing of Comamonas testosteroni strain B-356-biphenyl/chlorobiphenyl dioxygenase genes: evolutionary relationships among Gram-negative bacterial biphenyl dioxygenases.
In a previous work, all three components of Comamonas testosteroni B-356 biphenyl (BPH)/chlorobiphenyls (PCBs) dioxygenase (dox) have been purified and characterized. They include an iron-sulphur protein (ISPBPH) which is the terminal oxygenase composed of two subunits (encoded by bphA and bphE), a ferredoxin (FERBPH) encoded by bphF and a reductase (REDBPH) encoded by bphG. bphG Is not located in the neighbourhood of bphAEF in B-356. We are reporting the cloning of B-356-bphG and the sequencing of B-356-BPH dox genes. Comparative analysis of the genes provided genetic evidence showing that two BPH dox lineages have emerged in Gram-negative bacteria. The main features of the lineage that includes B-356 are the location of bphG outside the bph gene cluster and the structure of REDBPH which is very distinct from all other aryl dioxygenase-reductases.
Institut National de la Recherche Scientifique, INRS-Santé, Université du Québec, Pointe-Claire, Canada. michelvsylvestre@inrs-sante.uquebec.ca
M
M
Sylvestre
M
M
Sirois
Y
Y
Hurtubise
J
J
Bergeron
D
D
Ahmad
F
F
Shareck
D
D
Barriault
I
I
Guillemette
J M
JM
Juteau
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D049308:Dioxygenases; D005798:Genes, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics; D007506:Iron-Sulfur Proteins; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D010105:Oxygenases / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment
D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C074161:biphenyl-2,3-dioxygenase; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; D006867:Hydrolases; C062907:2,6-dioxo-6-phenylhexa-3-enoate hydrolase