Dysplasia expresses altered nuclear matrix protein composition in human ulcerative colitis.
We compared nuclear matrix protein compositions (NMPS) in colonic tissue from three different patient groups: 1) normal controls; 2) ulcerative colitis (UC); and 3) ulcerative colitis with dysplasia (UC-dysplasia). NMPS were separated by two-dimensional electrophoresis using isoelectrofocusing and SDS-PAGE. Six specific NMPS (59,500 Kd, pI 6.6 and 6.3; 49,250 Kd, pI 7.4; 33,750 Kd, pI 7.5; 20,000 Kd, pI 7.5; and 19,000 Kd, pI 6.3) were identified in UC-dysplasia that were not found in normal controls nor in UC colonic tissue. These observations suggest that NMPS may serve as important biochemical markers of dysplasia in UC.
Department of Medicine, Nassau County Medical Center, East Meadow, New York 11554, USA.
R S
RS
Izzo
C
C
Pellecchia
D003093:Colitis, Ulcerative / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000737:chemistry; D011230:Precancerous Conditions / Q000737:chemistry / Q000378:metabolism
D009687:Nuclear Proteins