Low expression of erythrocyte complement receptor type 1 in chronic hepatitis C patients.
Primate erythrocyte complement receptor type 1 (CR1) plays an essential role in complement-associated immune complex clearance by transporting complexes to macrophages in the liver and/or spleen. Antibody-bound hepatitis C virus, which consists of immune complexes, is observed in patients with chronic hepatitis C. The aim of this study was to clarify the pathophysiological roles of erythrocyte CR1 in hepatitis C virus-infected individuals. We quantified the expression of erythrocyte CR1 with a fluorescence-activated cell sorter system in 57 chronic hepatitis C and 37 chronic hepatitis B cases and 20 normal volunteers. Complement-bound immune complexes were quantified by means of an enzyme-linked immunosorbent assay using anti-C1q and anti-C3d antibodies. Hepatitis C virus-infected patients showed lower erythrocyte CR1 and higher C3d immune complex levels than volunteers (P < 0.01 and P < 0.05, respectively). An inverse correlation was observed between the erythrocyte CR1 and C3d immune complex levels in hepatitis C virus infection (r = -0.300, P = 0.032). The erythrocyte CR1 levels in hepatitis C virus infection were lower in patients with severe liver inflammation, cirrhosis, or hepatocellular carcinoma than in those with mild inflammation, whereas the levels did not differ regardless of the disease stage in hepatitis B virus infection. These findings demonstrate that the expression of erythrocyte CR1 is related to immune complex quantity and the severity of liver disease in hepatitis C virus infection.
First Department of Medicine, Osaka University Medical School, Suita, Japan.
T
T
Kanto
N
N
Hayashi
T
T
Takehara
K
K
Katayama
M
M
Kato
M
M
Akiyama
A
A
Kasahara
H
H
Fusamoto
T
T
Kamada
D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000936:Antigen-Antibody Complex / Q000276:immunology; D002908:Chronic Disease; D015922:Complement C1q / Q000276:immunology; D015933:Complement C3d / Q000276:immunology; D003450:Cryoglobulins / Q000276:immunology; D018450:Disease Progression; D004912:Erythrocytes; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006509:Hepatitis B / Q000276:immunology* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006526:Hepatitis C / Q000276:immunology* / Q000821:virology; D006801:Humans; D008099:Liver / Q000293:injuries / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000276:immunology*
D000936:Antigen-Antibody Complex; D003450:Cryoglobulins; D017463:Receptors, Complement 3b; D015922:Complement C1q; D015933:Complement C3d; D000410:Alanine Transaminase