Effects of in situ and systemic lindane treatment on in vivo absorption of galactose and leucine in rat jejunum.
In vivo intestinal absorption of L-leucine is significantly decreased by the presence of lindane (0.3, 0.2 and 0.1 mM) in perfusion solution (in situ lindane treatment) for 5 min. The inhibitory effect is earlier when lindane concentration is higher, and it is irreversible. There are no changes in D-galactose absorption when lindane (0.3 and 0.2 mM) is perfused for 5 min, but a significant decrease in observed if pesticide is perfused for 10 min at 0.3 mM concentration. Subcutaneous lindane treatment at a dose of 68.76 mumol/kg over 7 days does not alter D-galactose and L-leucine absorption. In situ lindane treatment (0.3, 0.2 and 0.1 mM) induces a significant decrease in basolateral (Na(+)-K(+)-ATPase activity. In contrast, systemic lindane treatment (s.c. injection) at doses of 34.38 and 68.76 mumol/kg over 7 days does not alter this enzyme activity, although a significant decrease is observed in rats injected s.c. with 68.76 mumol/kg lindane over 15 days.
Department of Physiology and Nutrition, Faculty of Pharmacy, University of Navarra, Pamplona, Spain.
M J
MJ
Moreno
S
S
Pellicer
M P
MP
Fernández-Otero
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005690:Galactose / Q000493:pharmacokinetics*; D001556:Hexachlorocyclohexane / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007441:Intubation, Gastrointestinal; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism*; D007930:Leucine / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001556:Hexachlorocyclohexane; D007930:Leucine; D005690:Galactose