Angiotensin II receptor type 1 on granulosa and thecal cells of rabbit preovulatory follicles.
Specific, high-affinity angiotensin II (A II) receptors were observed on granulosa and thecal cells of preovulatory ovarian follicles from immature PMSG-treated rabbits. Scatchard analysis of 125I-[Sar1,Ile8]A II binding to freshly prepared cells was indicative of only one class of binding sites. Kd values were 0.26 +/- 0.11 nM and 0.18 +/- 0.02 nM, densities of A II receptors were 0.06 +/- 0.02 fmol/10(5) cells and 0.08 +/- 0.01 fmol/10(5) cells for granulosa and thecal cells, respectively. When cells were incubated for 48 h with hCG, Kd values were of the same order of magnitude, but the amount of A II receptors was increased 2-fold in granulosa and 4-fold in theca. Using subtype specific ligands (Losartan for AT1 and PD 123319 for AT2) in competitive binding experiments, A II receptors were found to be of the AT1 type on both granulosa and thecal cells freshly prepared or incubated 48 h in vitro. These results establishing the existence of high affinity AT1 receptors on the two cell types of the rabbit preovulatory follicles contrast with previous observations showing the presence of AT2 receptors on granulosa or theca from several species.
Féral
C
C
Laboratoire de Biochimie, EP CNRS 9, CHU Côte de Nacre, Caen, France.
Benhaïm
A
A
Leymarie
P
P
D000804:Angiotensin II / Q000378:metabolism*; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D001665:Binding Sites; D001713:Biphenyl Compounds / Q000378:metabolism; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism; D007700:Kinetics; D019808:Losartan; D011725:Pyridines / Q000378:metabolism; D011817:Rabbits; D011945:Receptors, Angiotensin / Q000378:metabolism*; D012504:Saralasin / Q000378:metabolism; D013777:Tetrazoles / Q000378:metabolism; D013799:Theca Cells / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D057911:Angiotensin Receptor Antagonists; D001713:Biphenyl Compounds; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D007093:Imidazoles; D011725:Pyridines; D011945:Receptors, Angiotensin; D013777:Tetrazoles; D000804:Angiotensin II; C073402:PD 123319; D012504:Saralasin; D019808:Losartan