Cloning and sequencing of a cDNA encoding bovine intercellular adhesion molecule 3 (ICAM-3).
A cDNA encoding a putative bovine intercellular adhesion molecule (ICAM)-3, a ligand of the leukocyte integrin LFA-1 (CD11a/CD18), was sequenced and compared with human ICAM sequences. The 1635-bp bovine sequence codes for a protein of 544 amino acids (aa). This putative bovine ICAM-3 has five immunoglobulin (Ig)-like domains similar to human ICAM-1 and ICAM-3, and belongs to the Ig gene superfamily. The overall identities of the deduced aa sequence with those of human ICAM-3 and ICAM-1 are 61% and 58%, respectively. The predicted number and positions of Cys residues are all conserved between the bovine and human ICAM 3 aa sequences.
Lee
E K
EK
Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50010, USA.
Kehrli
M E
ME
Dietz
A B
AB
Bosworth
B T
BT
Reinhardt
T A
TA
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation; D001483:Base Sequence; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D004274:DNA, Recombinant; C483616:ICAM3 protein, human; D007074:Immunoglobulin G; D007136:Immunoglobulins; D018799:Intercellular Adhesion Molecule-1