Effect of interferon gamma and TNF alpha on the differentiation/activation of human glial cells: implication for the TNF alpha receptor 1.
This study focused on the activation/differentiation of human microglial cells and astrocytes, which is a prerequisite for HIV1 replication. In vitro, IFN gamma induced a differentiation-like morphological change in embryonic microglia and astrocytes, in both primary and in purified culture. This effect was enhanced by TNF alpha which in itself had no effect. IFN gamma did not increase the low level of endogenous TNF alpha, which is not secreted by embryonic cells, but stimulated the expression of TNF alpha R1, although to a relatively low extent. IFN gamma facilitated TNF alpha response through an increase in TNF alpha R1 expression, but probably also through interaction with different intracellular signal transduction pathways.
Laboratoire Virus, neurone et immunité, UFR Kremlin Bicêtre, France.
N
N
Janabi
A
A
Mirshahi
C
C
Wolfrom
M
M
Mirshahi
M
M
Tardieu
D001253:Astrocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D017628:Microglia / Q000187:drug effects* / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D007377:Interleukin-3; D008070:Lipopolysaccharides; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D002762:Cholecalciferol; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate