Rescue of Sendai virus cDNA templates with cDNA clones expressing parainfluenza virus type 3 N, P and L proteins.
Several years ago, we reported that a Sendai virus (SeV) defective genome (DIH4UV) could be rescued in vivo with human parainfluenza virus type 1 (hPIV1) and bovine PIV3 but not by measles virus or vesicular stomatitis virus. It was concluded that the cis-acting RNA sequences were conserved within the SeV/PIV1/PIV3 group but that interactions between the polymerase complex (P-L) and the template protein N were unique for each virus. We have re-examined these conclusions using proteins expressed from cloned N, P and L genes for SeV and PIV3. The results demonstrate the specificity of the protein-protein interactions between polymerase and template, and confirm the prediction of the earlier work that PIV3 N, P and L proteins are capable of assembling and replicating SeV mini-genomes also expressed from a cDNA clone.
Department of Genetics and Microbiology, University of Geneva Medical School (CMU), Switzerland.
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D000818:Animals; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D018076:DNA, Complementary; D004279:DNA, Viral; D006801:Humans; D019251:Nucleocapsid / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D012367:RNA, Viral; D018108:Respirovirus / Q000235:genetics*; D013698:Templates, Genetic; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000235:genetics*
D018076:DNA, Complementary; D004279:DNA, Viral; C095185:P protein, Human parainfluenza virus 3; D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C056831:parainfluenza 3 virus, L Protein