Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines.
Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in EHV-1 infected cells and expression was associated with enhanced levels of cell-cell fusion, indicating a role for EHV-1 gD in cell-to-cell transmission of virus.
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
J E
JE
Wellington
G L
GL
Lawrence
D N
DN
Love
J M
JM
Whalley
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D019084:Fluorescent Antibody Technique, Indirect; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D006736:Horses; D008322:Mammals; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D014162:Transfection; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis*
D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1