Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera.
A modified ELISA was developed for the detection of anti-Borrelia burgdorferi (Bb) IgG antibodies in wild animal sera based on an Enzyme-Labelled-protein G Assay (ELGA). Microplates were coated with an extract of Bb sensu stricto strain (SVI) as antigen. Specific antibodies of the serum samples were detected by a peroxidase-labelled-protein G. Using comparative immunodiagnosis by means of a passive hemagglutination test (HA), ELGA was tested on 82 roe-deer blood samples. A correlation was found between the two methods (r = 0.66). Good reproducibility of titers was observed by ELGA technique. A minimal cross-reactivity was discovered with Leptospira. ELGA could facilitate the recognition of specific antibodies in collections of wild animal sera.
Deruaz
D
D
Unité d' Ecologie des Systèmes Vectoriels, Institut Pasteur, Paris; France.
Eid
P
P
Deruaz
J
J
Sempéré
A
A
Bourgouin
C
C
Rodhain
F
F
Pérez-Eid
C
C
D000818:Animals; D000835:Animals, Wild / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood*; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D003670:Deer / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D006386:Hemagglutination Tests; D007074:Immunoglobulin G / Q000276:immunology*; D008193:Lyme Disease / Q000276:immunology / Q000662:veterinary; D021141:Nucleic Acid Amplification Techniques; D015203:Reproducibility of Results
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G