Differential response in the release of hydrogen peroxide between astroglial cells and endothelial cells following hypoxia/reoxygenation.
Hydrogen peroxide (H2O2) and lactate dehydrogenase (LDH) released from cultured astroglial cells (AGs) and brain endothelial cells (BECs) were measured following hypoxia/reoxygenation. AGs released H2O2 to the extracellular space from 8 h of hypoxia with the cellular injury indicated by LDH increase. In contrast, BECs released H2O2 at 4 h of hypoxia, prior to the LDH increase. These results suggest that AGs and BECs play different roles in production and metabolism of oxygen free radicals in the blood-brain barrier (BBB), and AGs may protect the BBB disruption from oxidative stress after hypoxia/reoxygenation.
Department of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.
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Kondo
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Kinouchi
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Kawase
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Yoshimoto
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D010100:Oxygen