Role of SOD-1 and nitric oxide/cyclic GMP cascade on neurofilament aggregation in ALS/MND.
A number of free radicals such as superoxide and nitric oxide may cause damage to motor neurons but the exact mechanism remains to be elucidated. A potent free radical, peroxynitrite, is readily formed from superoxide and nitric oxide, which captures superoxide three times faster than SOD-1. Peroxynitrite may nitrate tyrosine residues of light neurofilaments (NF-I), thereby altering NF assembly and causing NF accumulation in motor neurons. To test this hypothesis we have probed the massive NF aggregates which are histopathological hallmarks of ALS/MND with immunohistochemistry. We investigated localization of reaction products related to SOD-1, nitric oxide synthase (NOS) and cyclic GMP activities. Our studies show colocalization of NF aggregates with SOD-1/b-NOS/calmodulin /citrulline/cGMP and nitrotyrosine in upper motor neuron conglomerates (Cgl) and lower motor neutron axonal spheroids (Axs). This strongly supports the notion that peroxynitrite deranges NF phosphorylation and assembly, by nitrating tyrosine residues in NF-L. Impaired phosphorylation of NF subunits, either at NF-I or at NF-H, may affect the slow axonal transport culminating in proximo-distal accumulation of NF and slowly progressive motoneuron death.
Forbes Norris ALS/MDA Research Center, California Pacific Medical Center, San Francisco 94115, USA.
S M
SM
Chou
H S
HS
Wang
A
A
Taniguchi
D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism* / Q000503:physiopathology; D001369:Axons / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D002147:Calmodulin / Q000032:analysis; D002956:Citrulline / Q000032:analysis; D006152:Cyclic GMP / Q000502:physiology*; D005260:Female; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009046:Motor Neurons / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D016900:Neurofilament Proteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D017672:Nitrogen Compounds / Q000032:analysis; D013482:Superoxide Dismutase / Q000502:physiology*; D014443:Tyrosine / Q000032:analysis; D014452:Ubiquitins / Q000032:analysis
D002147:Calmodulin; D016900:Neurofilament Proteins; D017672:Nitrogen Compounds; D014452:Ubiquitins; C044882:neurofilament protein L; D002956:Citrulline; D009569:Nitric Oxide; D014443:Tyrosine; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D006152:Cyclic GMP