Increase in shedding of intercellular adhesion molecule-1 in human malignant melanoma cell lines treated with hyperthermia in vitro.
Human malignant melanoma cell lines were found to increase shedding of soluble intercellular adhesion molecule-1 (sICAM-1) into the culture medium when the cells were treated with hyperthermia at 41-43 degrees C for 3-6 hr in vitro. The content of ICAM-1 in the cell lysate was also found to be increased after hyperthermia. The increased rate of ICAM-1 concentration in the cells was at maximum when they were incubated at 41 degrees C for 3 hr. Also, the melanoma cell lines heat-treated at 41 degrees C showed more intense immunofluorescence in the ICAM-1 expression on the cell surface. It remains to be investigated further whether the effects of hyperthermia on the ICAM-1 expression in melanoma cells is to augment membrane ICAM-1 expression, which in turn leads to shedding of soluble ICAM-1 or only to acceleration of shedding of sICAM-1 by unknown mechanisms.
Department of Dermatology, Kyushu University Faculty of Medicine, Fukuoka, Japan.
J
J
Nakayama
T
T
Kageshita
M
M
Nakashima
M
M
Tsujisaki
K
K
Imai
Y
Y
Hori
D003600:Cytosol / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D006979:Hyperthermia, Induced; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism* / Q000628:therapy; D012995:Solubility; D014407:Tumor Cells, Cultured
D018799:Intercellular Adhesion Molecule-1