Reversal of the Z- to B-conformation of poly(dA-dT) center dot poly(dA-dT) induced by netropsin and distamycin A.
Poly(dA-dT) center dot poly(dA-dT) which adopts the Z-form at 5 M NaCl in presence of 95 mM Ni2+ions is reversed to the B-conformation by the nonintercalating drugs netropsin (Nt) and distamycin A (Dst). The drug-induced reversal from the Z-to B-form of poly(dA-dT) center dot poly(dA-dT) is evidenced by CD spectral changes at characteristic wavelengths around 295 nm and 248 nm. The drug-induced conformational transition is accompanied by a slow kinetic process. The results suggest the preference of these AT-specific drugs for the B-form and the inability of Nt and Dst to form a stable complex with the Z-form of poly(dA-dT) center dot poly(dA-dT).
Abteilung Molekulare Biologie, Institut fur Molekularbiologie der Friedrich-Schiller-Universitat Jena, Germany.
G
G
Burckhardt
A
A
Walter
C
C
Zimmer
D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology; D004214:Distamycins / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009429:Netropsin / Q000737:chemistry / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011067:Poly dA-dT / Q000737:chemistry*; D013997:Time Factors
D001426:Bacterial Proteins; D004214:Distamycins; D011067:Poly dA-dT; D009429:Netropsin; C030000:stallimycin