Molecular properties of enzymes involved in diacylglycerol and phosphatidate metabolism.
Diacylglycerol and phosphatidate serve as key lipid intermediates of glycerolipid biosynthesis and also as the second messengers liberated in signal-stimulated cells. The molecular properties of enzymes involved in the metabolic processing of the two lipids are largely unknown. We have revealed by cDNA cloning the presence of a novel enzyme family of diacylglycerol kinase (DGK) having unique structural characteristics. Furthermore, we recently identified the plasma membrane-bound phosphatidate phosphatase (PAP) as a 35 kDa glycoprotein. Detailed studies on the two enzymes would contribute to understanding the molecular mechanisms underlying the control of both glycerolipid biosynthesis and signal transduction.
Kanoh
H
H
Department of Biochemistry, Sapporo Medical University School of Medicine, Japan.
Kai
M
M
Wada
I
I
D000818:Animals; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism*; D006801:Humans; D010711:Phosphatidate Phosphatase / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015290:Second Messenger Systems; D015398:Signal Transduction
D004075:Diglycerides; D010743:Phospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; D010711:Phosphatidate Phosphatase