Collagen-induced arthritis in DBA/1 mice: cytokine gene activation following immunization with type II collagen.
Immunization of DBA/1 mice with bovine type II collagen (CII) in complete Freund's adjuvant (CFA) leads to collagen-induced arthritis as evidenced by joint inflammation. In this study, reverse transcription-polymerase chain reaction (RT-PCR) was used to demonstrate the activation of genes encoding for IL-2, IFN-gamma, and IL-10 in the lymph nodes from both CII-immunized and control CFA-immunized DBA/1 mice, at Days 10, 40, and 70 after immunization, in the absence of any IL-5 or IL-13 transcription. By quantitative RT-PCR, the levels of IFN-gamma mRNA in response to CII could not be quantitatively differentiated from the IFN-gamma transcribed in response to CFA alone. In the joints of CII-immunized mice, IL-1beta and IL-10 mRNA were found in the absence of IL-5 or IFN-gamma. Synovial IL-1beta and IL-10 were expressed most strongly at the time of clinical symptoms, 40 days after immunization. Together, these findings suggest that immunization with CII in CFA induces a type 1 response against the adjuvant, giving a cytokine environment which influences the T cells responding to CII to become type 1 T cells. This is manifested here by the appearance of gene activation in synovial tissue of collagen-immunized mice, but not in adjuvant-immunized control animals.
Stasiuk
L M
LM
INSERM Unité 283, Université René Descartes, Paris, France.
Abehsira-Amar
O
O
Fournier
C
C
D000818:Animals; D000941:Antigens / Q000276:immunology; D001168:Arthritis / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology; D001169:Arthritis, Experimental / Q000235:genetics / Q000276:immunology; D002417:Cattle; D003094:Collagen / Q000008:administration & dosage / Q000276:immunology; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D000941:Antigens; D016207:Cytokines; D007074:Immunoglobulin G; D007376:Interleukin-2; D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma; D003094:Collagen