Advances in sample preparation, electrophoretic separation and detection methods for rat cytochrome P450 enzymes.
A limited overview is given of the separation and detection of specific cytochrome P450 enzymes of the rat. Separation methods include group-specific chromatographic separation and electrophoretic separation in and elution from polyacrylamide gels. Detection methods that are considered include enzymatic analysis with and without chromatographic step using liquid chromatography and immunochemical methods following separation of the cytochrome P450 enzymes by polyacrylamide gel electrophoresis (Western blotting). The advantages and limitations of the various methods have been compared and discussed.
Laboratory for Health Effects Research, National Institute of Public Health and the Environment, Bilthoven, Netherlands.
E H
EH
Jansen
C A
CA
Laan
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de Fluiter
D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D008163:Luminescent Measurements; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D003577:Cytochrome P-450 Enzyme System