Differential effect of peroxisome proliferators on rat glutathione S-transferase isoenzymes.
We have investigated the effects of peroxisome proliferators silvex, nafenopin and diethylhexylphthalate (DEHP) on rat liver glutathione S-transferase (GST) isoenzyme activities and patterns. Silvex was a more potent in vitro GST inhibitor than nafenopin and DEHP. After 14 days oral administration to rats a reduction in total GST activity was observed, doses of compounds were chosen so that peroxisome proliferation was equivalent between compounds, nevertheless total GST activity was altered to different extents: nafenopin approximately silvex > DEHP approximately control. GST isoenzyme profiles were also altered, the proportion of GST 2-2 increased and 4-4 decreased compared to control levels. The results indicated that: (i) the peroxisome proliferators studied had similar effects on GST isoenzyme profile: (ii) modulation of the GST activity was apparently independent of peroxisome proliferation per se.
Key Centre for Applied and Nutritional Toxicology, RMIT-University, Melbourne, Vic, Australia.
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Voskoboinik
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Drew
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Ahokas
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism*; D006540:Herbicides / Q000633:toxicity*; D007527:Isoenzymes; D007700:Kinetics; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000201:enzymology; D009255:Nafenopin / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D004791:Enzyme Inhibitors; D006540:Herbicides; D007527:Isoenzymes; D009255:Nafenopin; D015085:2,4,5-Trichlorophenoxyacetic Acid; D004051:Diethylhexyl Phthalate; C009264:fenoprop; D005982:Glutathione Transferase