Increased deoxycytidine kinase activity by etoposide in L1210 murine leukemic cells.
Deoxycytidine kinase (dCK) is a rate-limiting enzyme for the activation of ara-C. It is responsible for the phosphorylation of ara-C which is widely used in the treatment of leukemia. We examined the effect of etoposide on dCK in L1210 cells and found that incubation with 10 microM etoposide for 1 h increased dCK activity to 170% of the control. This effect of etoposide on dCK activity was concentration-dependent up to at least 100 microM of the substance.
Department of Pharmacy, Yokkaichi Social Insurance Hospital, Mie, Japan.
K
K
Ooi
T
T
Ohkubo
M
M
Higashigawa
H
H
Kawasaki
M
M
Sakurai
D000818:Animals; D003561:Cytarabine / Q000378:metabolism; D003842:Deoxycytidine Kinase / Q000378:metabolism*; D005047:Etoposide / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000201:enzymology*; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D010766:Phosphorylation
D003561:Cytarabine; D005047:Etoposide; D003842:Deoxycytidine Kinase; D008727:Methotrexate