Dysplasia expresses altered nuclear matrix protein composition in human ulcerative colitis.
We compared nuclear matrix protein compositions (NMPS) in colonic tissue from three different patient groups: 1) normal controls; 2) ulcerative colitis (UC); and 3) ulcerative colitis with dysplasia (UC-dysplasia). NMPS were separated by two-dimensional electrophoresis using isoelectrofocusing and SDS-PAGE. Six specific NMPS (59,500 Kd, pI 6.6 and 6.3; 49,250 Kd, pI 7.4; 33,750 Kd, pI 7.5; 20,000 Kd, pI 7.5; and 19,000 Kd, pI 6.3) were identified in UC-dysplasia that were not found in normal controls nor in UC colonic tissue. These observations suggest that NMPS may serve as important biochemical markers of dysplasia in UC.
Izzo
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Department of Medicine, Nassau County Medical Center, East Meadow, New York 11554, USA.
Pellecchia
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D003093:Colitis, Ulcerative / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000737:chemistry; D011230:Precancerous Conditions / Q000737:chemistry / Q000378:metabolism
D009687:Nuclear Proteins