Genepool variation in genus Glycine subgenus Soja revealed by polymorphic nuclear and chloroplast microsatellites.
A combination of nuclear and chloroplast simple sequence repeats (SSRs) have been used to investigate the levels and pattern of variability detected in Glycine max and G. soja genotypes. Based on the analysis of 700 soybean genotypes with 115 restriction fragment length polymorphism (RFLP) probes, 12 accessions were identified that represent 92% of the allelic variability detected in this genepool. These 12 core genotypes together with a sample of G. max and G. soja accessions were evaluated with 11 nuclear SSRs that detected 129 alleles. Compared with the other G. max and G. soja genotypes sampled, the core genotypes represent 40% of the allelic variability detected with SSRs. Despite the multi-allelic nature of soybean SSRs, dendrograms representing phenetic relationships between accessions clustered according to their subspecies origin. In addition to biparentally inherited nuclear SSRs, two uniparentally (maternally) transmitted chloroplast SSRs were also studied. A total of seven haplotypes were identified, and diversity indices of 0.405 +/- 0.088 and 0.159 +/- 0.071 were obtained for the two chloroplast SSRs. The availability of polymorphic SSR loci in the chloroplast genome provides new opportunities to investigate cytonuclear interactions in plants.
Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland. wpowel@scri.scri.ac.uk
W
W
Powell
M
M
Morgante
J J
JJ
Doyle
J W
JW
McNicol
S V
SV
Tingey
A J
AJ
Rafalski
D002467:Cell Nucleus; D002736:Chloroplasts; D017343:Genes, Plant; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010802:Phylogeny; D013025:Glycine max / Q000145:classification / Q000235:genetics*