Fluorescence anisotropy: rapid, quantitative assay for protein-DNA and protein-protein interaction.
Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, Missouri 63104, USA.
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Heyduk
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Tang
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Ebright
D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D004247:DNA; D012321:DNA-Directed RNA Polymerases