Activation energy of the separation of DNA sequencing fragments in denaturing noncross-linked polyacrylamide by capillary electrophoresis.
We present the mobility of DNA sequencing fragments as a function of temperature in 5%T, 0%C noncross-linked polyacrylamide and at an electric field of 150 V/cm. The mobility of the fragments follows the Arrhenius equation. The activation energy for migration of a fragment through the polymer decreases monotonically with fragment length. The data were also analyzed in terms of the biased reptation model; the onset of biased reptation with stretching occurs for longer fragments as the temperature increase. These results are quite different from those observed for separation of DNA in cross-linked gels and represent a fundamental difference in the physics of the cross-linked and noncross-linked polyacrylamide.
Fang
Y
Y
Department of Chemistry, University of Alberta, Edmonton, Canada.
Zhang
J Z
JZ
Hou
J Y
JY
Lu
H
H
Dovichi
N J
NJ
D000180:Acrylic Resins / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D017422:Sequence Analysis, DNA; D013696:Temperature; D013816:Thermodynamics; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004247:DNA; D000099166:Polyacrylamides