Distribution of NADPHdiaphorase and calbindin-D28k neurons in the lateral septal area of the guinea pig, with special reference to the enkephalinergic hypothalamo-septal tract.
Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHd) histochemical techniques were used to identify neurons synthesizing nitric oxide in the lateral septum of the guinea pig. Double immunocytochemical procedures were used to detect neurons immunoreactive for calbindin-D28k and enkephalinergic fibers which project to the lateral septum. The present data demonstrate that (1) the neurons containing NADPH diaphorase and the neurons immunoreactive for calbindin-D28k are observed in discrete regions of the lateral septum; (2) these populations overlap in various areas of the lateral septum including its dorsal and mediolateral parts; (3) NADPH diaphorase and calbindin-D28k are colocalized in neurons located in the overlapping areas; (4) neurons identified by the presence of calbindin-D28k, NADPH diaphorase or both substances, are surrounded by enkephalinergic fibers. These observations indicate the chemical heterogeneity of the lateral septum and suggest that the enkephalinergic hypothalamo-septal tract does not preferentially contact a subpopulation of neurons.
Doutrelant-Viltart
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O
USTL, Laboratoire des Neurosciences du Comportement, Villeneuve d'Ascq, France.
Poulain
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P
D000818:Animals; D064026:Calbindins; D005260:Female; D006168:Guinea Pigs; D007031:Hypothalamus / Q000737:chemistry*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012688:Septum Pellucidum / Q000737:chemistry*; D014018:Tissue Distribution
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase