Two monoclonal antibodies from the platelet panel recognize sheep plasma fibrinogen.
Among the monoclonal antibodies (mAbs) submitted to the third Workshop, two mAbs, IVA120 (3W-323) and IVA198 (3W-290), could be identified to recognize the sheep fibrinogen molecule. The apparent molecular weight of the immunoprecipitated 48-60 kDa cell surface protein under reducing conditions suggested this antigen could be the fibrinogen molecule. ELISA and immunoblotting assays, performed with commercially available sheep plasma fibrinogen, confirmed that these two mAbs recognize two different epitopes present on the sheep fibrinogen molecule.
Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Spain.
J M
JM
Pérez de la Lastra
A
A
Mateo
R
R
Dusinsky
M
M
Simon
D
D
Llanes
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification* / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D005340:Fibrinogen / Q000276:immunology*; D015151:Immunoblotting; D011233:Precipitin Tests; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology*; D012756:Sheep
D000911:Antibodies, Monoclonal; D011498:Protein Precursors; D005340:Fibrinogen