Rapid genotypic confirmation of methicillin resistance.
Detection of phenotypic methicillin resistance in Staphylococcus aureus clinical strains by conventional disk diffusion testing is fraught with problems. We used gene amplification of the mecA locus by polymerase chain reaction (PCR), in conjunction with a capillary/air thermal cycler, to overcome both the inaccuracy of phenotypic methods and the lengthy processing times required for previous genotypic methods. The rapid PCR method correctly identified methicillin resistance in a consecutive series of 30 S. aureus isolates when compared with routine and reference phenotypic methods. The shorter processing time and smaller reagent volumes required for the air thermal cycler make same-day determination of methicillin resistance in clinical isolates feasible for diagnostic laboratories.
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
T J
TJ
Inglis
W
W
Rahman
D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics
D004269:DNA, Bacterial