Increase of murine splenic natural antibody-secreting cells after cyclophosphamide treatment.
Administration of a low sub-immunosuppressive dose of cyclophosphamide (CY) to naive mice induced a marked increase in the number of splenic cells forming natural antibodies against unrelated antigens such as foot-and-mouth disease virus, keyhole limpet hemocyanin, horseradish peroxidase, or bovine serum albumin, as determined by an enzyme-linked immunosorbent assay spot technique. These results suggest that in mice there exists a repertoire of B cells forming natural antibodies which is restrained by an unknown mechanism and that CY is able to interfere with that restriction.
Cátedra de Patología General Veterinaria, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
E L
EL
Portiansky
P H
PH
González
R P
RP
Laguens
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology*; D002452:Cell Count / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology*
D003520:Cyclophosphamide