Structural analysis of N-linked oligosaccharide of mitogenic lectin-B from the roots of pokeweed (Phytolacca americana).
The structure of an asparagine (N-) linked oligosaccharide of pokeweed lectin-B (PL-B) and the amino acid sequences around two glycosylation sites were identified. The pyridylamino (PA) oligosaccharide prepared from PL-B was eluted as a single peak on both reversed-phase (RP-) HPLC and size-fractionation (SF-) HPLC, and its structure was estimated to be Man alpha 1-->6(Man alpha 1-->3)(Xyl beta 1-->2) Man beta 1-->4GlcNAc beta 1-->4(Fuc alpha 1-->3)GlcNAc by a combination of component analysis, successive exoglycosidase digestions, IS-MS analysis, and 500 MHz 1H-NMR. Two tryptic glycopeptides were isolated from the reduced and S-pyridylethylated PL-B after gel filtration followed by RP-HPLC, indicating the presence of two glycosylation sites in PL-B. The amino acid sequences around the two glycosylation sites were determined to be Cys-Gly-Val-Asp-Phe-Gly-Asn(CHO)-Arg.
Department of Bioresources Chemistry, Okayama University, Japan.
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Kimura
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Yamaguchi
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Funatsu
D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D037102:Lectins / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D037121:Plant Lectins; D018517:Plant Roots / Q000737:chemistry; D011043:Pokeweed Mitogens / Q000032:analysis* / Q000737:chemistry
D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D011043:Pokeweed Mitogens