Segmental diversification of an identified leech neuron correlates with the segmental domain in which it expresses Lox2, a member of the Hox gene family.
The cellular colocalization of LOX2 protein and small cardioactive peptide (SCP)-like immunoreactivity was studied in the nerve cord of the glossiphoniid leech Helobdella triserialis. Of the six neurons that express SCP in the midbody segments 7 to 17, only one, the MPS neuron, expresses LOX2 protein. The medial paired SCP (MPS) neurons are segmentally repeated and can be divided into three contiguous segmental domains according to cell body size and the timing and level of SCP expression. MPS neurons located in the anterior and middle segmental domains express LOX2 protein. In the middle domain, large MPS neurons begin to accumulate SCP shortly after the end of embryonic development, whereas in the anterior domain the MPS neurons are smaller and begin to express SCP at a later stage. In the posterior domain the MPS neurons exhibit a third phenotype -- they have large cell bodies, express low levels of SCP starting from the midjuvenile stage, and do not show detectable LOX2 expression. Lineage tracer injections showed that the MPS neurons arise from a stereotyped cell lineage and are descended from the O teloblast stem cell. In midbody ganglia 2 to 6 and 18 to 21, there are lineally homologous neurons that do not express either LOX2 protein or SCP. Thus, the boundaries of LOX2 expression coincide precisely with two of the segmental boundaries of MPS differentiation, suggesting that expression of LOX2 at the level of this single identified neuron governs some, but not all, aspects of the neuron's segmental diversification.
Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
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Berezovskii
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Shankland
D000818:Animals; D000918:Antibody Specificity; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005801:Genes, Homeobox / Q000502:physiology; D018398:Homeodomain Proteins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D007447:Invertebrate Hormones / Q000032:analysis; D007865:Leeches / Q000166:cytology / Q000196:embryology* / Q000235:genetics; D008297:Male; D009457:Neuroglia / Q000737:chemistry / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009479:Neuropeptides / Q000032:analysis* / Q000276:immunology; D013234:Stem Cells / Q000737:chemistry / Q000502:physiology
D018398:Homeodomain Proteins; D007447:Invertebrate Hormones; D009479:Neuropeptides; C045659:small cardioactive peptide A