Reduction by indobufen of neutrophil activation in peripheral arterial occlusive disease.
We evaluated the effectiveness of indobufen administration in reducing neutrophil activation in a clinical model of ischemia-reperfusion. Thirty stable patients with intermittent claudication due to occlusive peripheral arterial disease of the leg were randomly assigned to two groups. Patients in group I were treated with indobufen [200 mg orally twice daily (p.o. b.i.d.) for a week]; patients in group II received a placebo. Both groups of patients were submitted to standardized treadmill exercise until onset of claudication. Plasma levels of thromboxane B2 (TxB2) and 6-keto-prostaglandin F1alpha(6-k-PGF1alpha) neutrophil filterability, and neutrophil activation (by nitro-blue tetrazolium test) were assessed in blood samples from the femoral vein draining the ischemic leg. The values were obtained at rest and 5, 30, and 60 min after onset of claudication. Urinary albumin excretion was measured at rest and 1 h after onset of claudication. Plasma levels of TxB2 and 6-k-PGF1alpha increased significantly in the placebo group 5 min after onset of claudication, whereas only a slight nonsignificant increase was observed in the indobufen-treated group at the same timepoint.
Dipartimento di Medicina Sperimentale e Clinica, Facolta di Medicina e Chirurgia, Catanzaro, Italy.
M
M
Chello
P
P
Mastroroberto
V
V
Celi
F
F
Romano
A R
AR
Marchese
A
A
Colonna
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D001157:Arterial Occlusive Diseases / Q000097:blood / Q000188:drug therapy*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007383:Intermittent Claudication / Q000097:blood / Q000188:drug therapy; D054833:Isoindoles; D019344:Lactic Acid / Q000378:metabolism; D008875:Middle Aged; D018375:Neutrophil Activation / Q000187:drug effects*; D010654:Phenylbutyrates / Q000494:pharmacology* / Q000627:therapeutic use; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis
D016861:Cyclooxygenase Inhibitors; D054833:Isoindoles; D010654:Phenylbutyrates; D010975:Platelet Aggregation Inhibitors; D019344:Lactic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C020371:indobufen