Plasmatic and membrane lipid alterations in erythrocytes from diabetic rats fed either n-6 or n-3 fatty acids.
We examined the effect of dietary n-6 and n-3 fatty acids on the lipid composition and physical properties of erythrocyte membranes together with cholesterol and triglyceride plasmatic levels in normal and experimental diabetic rats. Plasmatic total cholesterol and triglyceride did not change in normal rats under the dietary regime, but both parameters decreased significantly in the diabetic animals after the consumption of either n-6 or n-3 fatty acids. Lipid analyses of erythrocyte membranes revealed a significant decrease in the total cholesterol together with an increase in the phospholipid amount in the diabetics compared to the normal rats. As a consequence, cholesterol/phospholipid ratio decreased in these groups of animals and markedly in those fed n-3 fatty acids. These changes would be responsible for the lower fluorescent polarization of DPH observed in the latter group. We conclude that equivalent and adequate amounts of dietary either n-6 or n-3 fatty acids produce plasmatic and red cell membrane lipid changes in diabetic rats that may improve the evolution of the disease.
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, Argentína.
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de Gómez Dumm
D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001798:Blood Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004161:Diphenylhexatriene / Q000737:chemistry / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005453:Fluorescence; D006031:Glycosylation; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood* / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D001798:Blood Proteins; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D014280:Triglycerides; D004161:Diphenylhexatriene; D002784:Cholesterol