A reliable and sensitive enzyme immunoassay method for measuring 8-isoprostaglandin F2 alpha: a marker for lipid peroxidation after experimental brain injury.
The objectives of this study were to determine (1) if levels of 8-isoprostaglandin F2 alpha (8-isoPGF2 alpha), a non-enzymatically produced prostaglandin, were increased after cortical contusion and (2) if enzyme immunoassay (EIA) could be used to quantify 8-isoPGF2 alpha levels. 24 h after the contusion there was a significant rise in 8-isoPGF2 alpha compared to control levels. The levels returned to baseline by 72 h postinjury. The results show that this EIA method is a reliable and sensitive technique for measuring brain lipid peroxidation.
Brain Research Laboratory, Rutgers University, Newark, NJ 07102, USA.
S W
SW
Hoffman
R L
RL
Roof
D G
DG
Stein
D000818:Animals; D015415:Biomarkers; D001930:Brain Injuries / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D015227:Lipid Peroxidation / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D015232:Dinoprostone