IL-5 receptor expression and Ig secretion from murine B lymphocytes requires coordinated signaling by membrane Ig, IL-4, and IL-5.
Interleukin-5 plays a pivotal role in the regulation of Ig secretion from murine B cells. Resting B cells express few, if any, IL-5 receptors and do not respond to the lymphokine. Culture of resting B cells with IL-4 induced expression of the IL-5R alpha-chain, while signaling through membrane Ig stimulated expression of the IL-5R beta-chain. Surprisingly, IL-4 suppressed expression of the beta-chain on activated B cells and inhibited responsiveness to IL-5 in subsequent cultures. Simultaneous culture of B lymphoblasts with IL-4 and IL-5 elicited sustained expression of the beta-chain and promoted secretion of IgM. Thus, expression of IL-5 receptors and induction of Ig secretion requires coordination of signals provided by membrane Ig, IL-4, and IL-5.
Monsanto Company/Searle Research and Development, Immunoinflammatory Diseases Department, St. Louis, MO 63198, USA.
J D
JD
Weber
P C
PC
Isakson
J M
JM
Purkerson
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D007075:Immunoglobulin M / Q000096:biosynthesis; D015847:Interleukin-4 / Q000502:physiology*; D015848:Interleukin-5 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D053649:Receptors, Interleukin-5; D015398:Signal Transduction / Q000276:immunology*
D007075:Immunoglobulin M; D015848:Interleukin-5; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D015847:Interleukin-4