Immunohistochemical studies of proinflammatory cytokines and their receptors in hair follicles of normal human skin.
Immunoreactivity to interleukin-1 alpha, interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha and their receptors, as well as the endogenous interleukin-1 receptor antagonist, was investigated in hair follicles in paraffin-embedded normal human skin. Interleukin-1 beta- and tumour necrosis factor-alpha-like immunoreactivities were found in the inner root sheath layer of hair follicles, at the suprapapillary level. Interleukin-1 receptor-like immunoreactivity was also found in this layer, while there was a variable immunoreactivity to the interleukin-1 receptor antagonist. In the outer root sheath there was a weak to moderate staining for the four cytokines, in addition to intense staining for their receptors and a weak staining for the antagonist. The fibrous root sheath had a moderate immunoreactivity for interleukin-1 alpha and interleukin-6. The distribution patterns suggest that these cytokines, particularly interleukin-1 beta and tumour necrosis factor-alpha, may have a protective role in hair formation, while all the investigated proinflammatory cytokines may have a role in the differentiation process.
Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
A A
AA
Ahmed
K
K
Nordlind
M
M
Schultzberg
J
J
Brakenhoff
J
J
Bristulf
D
D
Novick
S B
SB
Svenson
M
M
Azizi
S
S
Lidén
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D005260:Female; D018859:Hair Follicle / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007378:Interleukins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D018123:Receptors, Interleukin / Q000032:analysis*; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000037:antagonists & inhibitors; D019947:Receptors, Interleukin-6; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D015703:Antigens, CD; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D018123:Receptors, Interleukin; D017472:Receptors, Interleukin-1; D019947:Receptors, Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha