Cell surface display of a single-chain antibody for attaching polypeptides.
To provide an efficient means of coupling proteins, peptides and other suitable moieties to cells, we have constructed a retroviral expression vector for cell surface display of a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-oxazo-line-5-one (phOx). The hapten phOx can be easily conjugated to primary amino and sulfhydryl groups, thus providing points of attachment for the cell surface-bound anti-phOx scFv. This universal cell coupling system could prove to be particularly useful for anchoring monoclonal antibodies for tumor targeting and to present co-stimulatory molecules and other ligands (even mixtures) at the cell surface for gene therapy.
Rode
H J
HJ
German Cancer Research Center, Heidelberg, Germany.
Little
M
M
Fuchs
P
P
Dörsam
H
H
Schooltink
H
H
de Inés
C
C
Dübel
S
S
Breitling
F
F
D000818:Animals; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured; D005434:Flow Cytometry; D006241:Haptens / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000235:genetics* / Q000276:immunology; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D006241:Haptens; D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv