Variable expression of Epstein-Barr virus latent membrane protein I in Reed-Sternberg cells of Hodgkin's disease.
Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.
Department of Pathology, Okayama University Medical School, Japan.
N
N
Teramoto
L
L
Cao
N
N
Kawasaki
Y
Y
Tonoyama
A B
AB
Sarker
T
T
Yoshino
K
K
Takahashi
T
T
Akagi
D004854:Herpesvirus 4, Human / Q000378:metabolism / Q000502:physiology*; D006689:Hodgkin Disease / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016539:Reed-Sternberg Cells / Q000821:virology*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D017735:Virus Latency
D014763:Viral Matrix Proteins