Analysis of T cell receptor beta chains in the rat: I. Allelic polymorphism of V beta 8.2 is not a predisposing genetic factor in susceptibility to experimental allergic encephalomyelitis.
Previous studies of experimental allergic encephalomyelitis (EAE) in the LER rat have suggested that amino acid differences present in the LER TCR V beta 8.2 chain may be associated with disease resistance. We report here that LEW rats bred to express a V beta 8.2 gene from DA rats, identical to that found in LER, are susceptible to EAE induction. Furthermore, T cells infiltrating the spinal cord of diseased animals primarily utilized V beta 8.2 and the associated AspSer CDR3 motif, typical of TCR V beta 8.2 chains expressed by pathogenic, anti-MBP responsive T cells in the LEW rat.
Sidney Kimmel Cancer Center, San Diego, California 92014, USA.
D P
DP
Gold
K
K
Schroder
D B
DB
Mueller
D B
DB
Wilson
D000483:Alleles; D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000378:metabolism; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D011110:Polymorphism, Genetic; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D013116:Spinal Cord / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta