Molecular motor mechanics in the contracting heart. V1 versus V3 myosin heavy chain.
The amount of iron in the low molecular weight pool (LMW) increases during no-flow ischemia and is thought to be essential to oxygen radical-derived damage upon reperfusion. Applying three short ischemic periods (5 min) preconditioning before 15 min ischemia results in an improved contractility compared to a direct 15 min ischemic insult. This raises the question whether preconditioning leads to a decrease in hte LMW iron pool. We therefore investigated the change in in hte LMW iron pool during ischemic insult after applying preconditioning. It is assumed that an increase in LMW iron is dependent on the accumulation of reduction equivalents derived from the anaerobic glycolysis. Therefore the glycogen content was also reduced by administration by anoxia and glucagon administration to study the effect on the LMW iron pool.
Peterson
J N
JN
Department of Molecular Physiology and Biophysics, University of Vermont College of Medicine, Burlington 05405, USA.
Alpert
N R
NR
D000818:Animals; D007527:Isoenzymes / Q000502:physiology*; D007700:Kinetics; D009200:Myocardial Contraction / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000502:physiology*; D011817:Rabbits
D007527:Isoenzymes; D018995:Myosin Heavy Chains