Sialyl Lewis(x)/E-selectin-mediated rolling in a cell-free system.
Selections mediate transient adhesion of neutrophils to stimulated endothelial cells at sites of inflammation by binding counter-receptors that present carbohydrates such as sialyl Lewis(x). We have developed a cell-free adhesion assay using sialyl Lewis(x)-coated microspheres and E-selection-IgG chimera-coated substrates to investigate the premise that rolling primarily results from functional properties of selection-carbohydrate bonds, whereas cellular morphology and signaling act as secondary effects. Sialyl Lewis(x)-coated microspheres attach to and roll over E-selectin-IgG chimera-coated substrates between the physiological wall shear stresses of 0.7 and 2 dynes/cm2. Rolling velocities vary with time and depend on E-selectin-IgG chimera site density and wall shear stress. Our results show that sialyl Lewis(x) is a minimal functional recognition element required for rolling on E-selectin under flow.
Brunk
D K
DK
School of Chemical Engineering, Cornell University, Ithaca, New York 14853, USA.
Goetz
D J
DJ
Hammer
D A
DA
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002474:Cell-Free System; D019040:E-Selectin / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology; D016256:Lewis X Antigen / Q000502:physiology*; D008863:Microspheres; D008954:Models, Biological; D009504:Neutrophils / Q000166:cytology*; D009844:Oligosaccharides / Q000737:chemistry; D000080506:Sialyl Lewis X Antigen; D013314:Stress, Mechanical
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen