Iloprost effects on phagocytes in patients suffering from ischaemic diseases: in vivo evidence for down-regulation of alpha M beta 2 integrin.
This study has been designed to demonstrate the in vivo effects of iloprost therapy on expression of adhesion molecules on phagocytes. Sixty patients suffering from peripheral arterial occlusive disease (PAOD) and/or from skin ulcers due to secondary progressive systemic sclerosis (PSS) were enrolled in a double-blind controlled parallel study. Thirty patients (group I) underwent iloprost infusion and 30 patients (group II) were treated with aspirin. Clinical assessment and measurement of phagocyte activation in vivo, using quantitative flow cytometry, were performed on entry and after 6 h on the first day of therapy. After 3 months of therapy, complete healing of all cutaneous lesions was observed in 84% of the patients treated with iloprost compared with the control patients (P < 0.001). Neutrophils and monocytes of PAOD and PSS patients showed a significant decrease in the expression of the alpha M beta 2 integrin adhesion receptor after 6 h of iloprost infusion. Neutrophils and monocytes released a lower amount of anion superoxide (O2-) after 6 h of iloprost treatment. These data confirm other clinical observations but demonstrate that in vivo this drug modifies the expression of the alpha M beta 2 integrin of phagocytes that has a key role in leukocyte-endothelium interactions in cases of inflammation and thrombosis.
Department of Internal Medicine, University of Pavia, Italy.
A
A
Mazzone
I
I
Mazzucchelli
G
G
Fossati
D
D
Gritti
S
S
Girola
C
C
Canale
C
C
Cusa
G
G
Ricevuti
D000328:Adult; D000368:Aged; D001157:Arterial Occlusive Diseases / Q000188:drug therapy*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D016285:Iloprost / Q000494:pharmacology* / Q000627:therapeutic use; D016177:Macrophage-1 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010586:Phagocytes / Q000187:drug effects*; D012595:Scleroderma, Systemic / Q000188:drug therapy*; D012881:Skin Temperature / Q000187:drug effects
D016177:Macrophage-1 Antigen; D016285:Iloprost