Phenotypic correction of dwarfism by constitutive expression of growth hormone.
GH is normally secreted in a pulsatile fashion. When GH is deficient, dwarfism is the result in both rodents and humans. An adenoviral vector containing the rat GH complementary DNA was used to induce constitutive GH expression in hepatocytes of GH-deficient lit/lit mice. Elevated serum GH increased circulating insulin-like growth factor I concentrations, corrected the growth deficiency, and normalized body composition. The results indicate that correction of the dwarf phenotype can be achieved by constitutive expression of GH at an ectopic site by gene transfer.
Hahn
T M
TM
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Copeland
K C
KC
Woo
S L
SL
D000260:Adenoviruses, Human; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D018076:DNA, Complementary; D004392:Dwarfism / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy*; D015316:Genetic Therapy; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis* / Q000097:blood / Q000172:deficiency*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D009929:Organ Size; D010641:Phenotype; D051381:Rats; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis
D018076:DNA, Complementary; D000515:alpha 1-Antitrypsin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone