[NMR and other physical methods].
Solid-state NMR can be used for the samples under limited motions such as membrane systems with membrane transport proteins. Nuclear spin interactions such as the chemical shift anisotropy, dipole-dipole interaction and quadrupole interaction can provide the structural information. To get the information at high resolution, the sample should be either oriented or rotated at the magic angle. New methods are developed to recover the nuclear spin interactions under magic angle spinning. By using specifically deuterated phospholipids, detailed information on the protein-lipid interactions can be obtained from the quadrupole splittings of these phospholipids. X-ray crystallography has contributed to the structural analysis of membrane transport proteins after the crystallization method in the presence of detergents was developed.
Yokohama National University, Faculty of Engineering, Japan.
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Akutsu
D000818:Animals; D002352:Carrier Proteins; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids; D010743:Phospholipids; D011487:Protein Conformation
D002352:Carrier Proteins; D008563:Membrane Lipids; D010743:Phospholipids