Long-term culture of fully differentiated adult insect neurons.
An identified group of cells, dorsal unpaired median (DUM) neurons, isolated from the central nervous system of adult cockroaches can be grown in vitro for extended periods. These cells often develop morphological characteristics that differ from their in situ appearance but physiological experiments demonstrate that they retain their distinctive in vivo membrane properties. A method for culturing insect CNS neurons, which includes the use of a haemolymph-derived growth-enhancing factor is described, and this technique is compared with other attempts to develop an efficient system for producing in vitro preparations of well-defined, identifiable neurons that would provide a model system for aspects of neuron developmental growth, repair and function.
National Vibrating Probe Facility, Marine Biological Laboratory, Woods Hole, MA 02543, USA. psmith@mbl.edu
P J
PJ
Smith
E A
EA
Howes
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D003058:Cockroaches; D009474:Neurons / Q000502:physiology*; D013997:Time Factors