Lymphocyte muscarinic cholinergic activity and PGE2 involvement in experimental Trypanosoma cruzi infection.
In this paper, we demonstrated that the production of PGE2 by CD8+ T lymphocytes through muscarinic cholinergic receptor (mAChR) activation of lymphocytes from mice acutely infected with nonlethal Trypanosoma cruzi CA-1 strain could enhance resistance to infection. Treatment in vivo with either atropine or cyclooxygenase inhibitors enhanced mortality rates and parasitemia of mice infected with T. cruzi CA-1 strain. The mechanism by which CD8+ T lymphocytes released PGE2 appears to involve the activation of the cells by circulating IgG present in mice infected with T. cruzi CA-1 strain. Binding of these antibodies to mAChR on CD8+ T lymphocytes triggered the release of large amounts of PGE2. The results point to a role of serum antibodies against mAChR in the protection of T. cruzi infection. The prostanoid acting as an immunomodulator contributed to the maintenance of the chronic course of experimental Chagas disease.
CEFYBO-CONICET, Cátedras de Microbiologia y Farmacologia, Facultad de Medícina y de Odontologia, Universidad de Buenos Aires, Argentina.
L
L
Sterin-Borda
G
G
Gorelik
N
N
Goren
S G
SG
Cappa
A M
AM
Celentano
E
E
Borda
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D002462:Cell Membrane / Q000469:parasitology; D014355:Chagas Disease / Q000097:blood / Q000276:immunology*; D015232:Dinoprostone / Q000097:blood / Q000494:pharmacology*; D007113:Immunity, Innate; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018727:Muscarinic Antagonists / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000276:immunology / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000302:isolation & purification
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D018727:Muscarinic Antagonists; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D001285:Atropine; D015232:Dinoprostone