Immunocytochemical localization of 60-kDa heat shock protein in Vibrio cholerae.
The immunocytochemical localization of the 60-kDa heat shock protein (HSP) in Vibrio cholerae strain 569B was studied by transmission electron microscopy using a combination with the antigen-specific monoclonal antibody (5C3) and immunogold labelling. The labelling with gold particles in V. cholerae detected 2 types; the gold particles were exclusively detected in the cytoplasm for one type and in the periplasmic space for another type, suggesting that the 60-kDa HSP of V. cholerae corresponding immunologically to Escherichia coli GroEL may translocate from the cytoplasm to the periplasmic space in the V. cholerae cell.
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
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H
Taguchi
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H
Yamaguchi
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T
Yamamoto
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Kamiya
D000818:Animals; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D014734:Vibrio cholerae / Q000378:metabolism*
D001426:Bacterial Proteins; D018834:Chaperonin 60