Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706.
We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the alpha (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.
Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA.
A A
AA
Franco
P E
PE
Yeh
J A
JA
Johnson
E M
EM
Barry
H
H
Guerra
R
R
Maurer
J G
JG
Morris
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004258:DNA Polymerase III / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D014734:Vibrio cholerae / Q000201:enzymology / Q000235:genetics*
C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III