Distinct cellular compartment of cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activator protein (p35nck5a) in the developing rat cerebellum.
We have elucidated the spatial and temporal localization of Cdk5 and p35nck5a in the developing rat postnatal cerebellum. Both proteins were highly expressed in cell bodies of post mitotic and immature neurons. The localization of Cdk5 in cellular compartment was changed from cell body to the axon in development. On the other hand, p35nck5a was always expressed in the cell body throughout cerebellum development. The Cdk5 kinase activity was correlated with the expression of p35nck5a rather than that of Cdk5. These results indicate that p35nck5a is a physiological activator of Cdk5 in immature neurons and further suggest that Cdk5 has another function in mature neurons.
First Department of Physiology, Okayama University Medical School, Japan.
M
M
Matsushita
K
K
Tomizawa
Y F
YF
Lu
A
A
Moriwaki
M
M
Tokuda
T
T
Itano
J H
JH
Wang
O
O
Hatase
H
H
Matsui
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D015153:Blotting, Western; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins; C089495:neuronal Cdk5 activator (p25-p35); D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495907:Cdk5 protein, rat; D018844:Cyclin-Dependent Kinases