[Three-dimensional analysis of chromosomes by a computerized fluorescence microscope system].
In order to study spatial arrangement and dynamics of chromosomes and nuclear structures, we have developed a computerized fluorescence microscope workstation. This microscope workstation is capable of rapidly acquiring three-dimensional, multiple-wavelength image data and allows accurate reconstructions of three-dimensional cellular structures stained with multiple fluorescent dyes. Using this microscope system, we have examined dynamics of chromosomes and microtubules in mammalian culture cells by combination of high-resolution analysis in fixed specimens and time-lapse analysis in living cells. A key aspect of such microscopic approaches is a capability of detecting transient events during dynamic biological processes.
Haraguchi
T
T
Kansai Advanced Research Center, Communications Research Laboratory.
Hiraoka
Y
Y
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*