Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.
To examine the susceptibility of the epithelial cell line to rat coronavirus (RCV), we inoculated sialodacryoadenitis virus and Parker's RCV into five cell lines; JTC-19, rat L2, LLC, RCN-9 and LBC cells originating in the lungs, intestines and mammary tumors of rodents. Both RCVs were replicated in LBC and RCN-9 cells, but not in the others. The infectivity titers of both RCVs grown in RCN-9 cells were significantly higher than those in LBC cells in every passage (2.5-3.9 log rate). Both RCVs replicated in LBC cells showed higher tropism to RCN-9 cells than to LBC cells, suggesting that RCN-9 cells are more suitable for the replication of RCVs than LBC cells. The RCN-9 cell line would be useful for the investigation of RCV infection in rodents.
Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.
K
K
Ohsawa
Y
Y
Watanabe
A
A
Takakura
T
T
Itoh
H
H
Sato
D000818:Animals; D002460:Cell Line; D017941:Coronavirus, Rat / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D004847:Epithelial Cells; D015726:Giant Cells; D007422:Intestines / Q000166:cytology; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D012376:Rodent Diseases / Q000821:virology; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000502:physiology*