An improved method to distinguish micrococcal nuclease sensitivity of chromatin.
The resolution of the standard micrococcal nuclease assay for sensitivity of active chromatin has been enhanced by the inclusion of an additional step of digesting nuclease-digested DNA with a suitable restriction enzyme prior to Southern hybridization. The improved assay has been used to analyze the chromatin structure of the lamin A, albumin and alpha-fetoprotein genes during rat liver development.
Centre for Cellular and Molecular Biology, Hyderabad, India.
Q A
QA
Hamid
S
S
Thanumalayan
V K
VK
Parnaik
D000818:Animals; D015139:Blotting, Southern; D002454:Cell Differentiation / Q000502:physiology; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D003850:Deoxyribonuclease I / Q000378:metabolism; D034904:Lamin Type A; D034882:Lamins; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D002843:Chromatin; D015342:DNA Probes; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease