Application of antibody adsorbed polyester cloth for the rapid screening of elution conditions for antigen immunopurification.
Antibody adsorbed onto polyester cloth did not desorb during a 20 hour agitated incubation in commonly used antigen-antibody dissociating agents. Antibody activity on the cloth was also unaffected by five 20 min cycles of agitation in these agents. Use of cloth segments coated with a model antibody, goat anti-rabbit IgG antibody, permitted rapid screening of elution conditions for the immunoaffinity purification of a model antigen, rabbit IgG. Thus, this simple test is useful for designing an optimal immunoaffinity chromatographic procedure.
Department of Biology, Carleton University Ottawa, Canada.
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Boyd
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Yamazaki
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification / Q000378:metabolism*; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D007074:Immunoglobulin G / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D011091:Polyesters; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011091:Polyesters; C048844:anti-IgG