Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
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Howard Hughes Medical Institute, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.
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D003001:Cloning, Molecular; D004274:DNA, Recombinant; D015723:Gene Library; D005816:Genetic Complementation Test; D005819:Genetic Markers; D014644:Genetic Variation; D005822:Genetic Vectors / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D010957:Plasmids; D012568:Schizosaccharomyces / Q000235:genetics*
D004274:DNA, Recombinant; D005819:Genetic Markers; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase