Effect of environmental tobacco smoke on LDL accumulation in the artery wall.
BACKGROUND
Previous research has shown that exposure to environmental tobacco smoke (ETS) increases the risk of atherosclerosis. To test the hypothesis that exposure to ETS increases LDL accumulation in the artery wall, we developed a model to measure the rate of LDL accumulation in individually perfused rat carotid arteries after the artery had been perfused with plasma taken from rats exposed to ETS (ETS-plasma).


RESULTS
Rats were exposed to ETS in a chamber in which steady-state sidestream smoke was continuously circulating. After exposure, blood from the animals was collected. Carotid arteries from unexposed rats were perfused first with normal plasma containing fluorescently labeled LDL. Then the same arteries (10 arteries from five rats) were perfused with ETS-plasma plus fluorescently labeled LDL. Photometric measurements were made during perfusion of the arteries with fluorescently labeled LDL, and rate of LDL accumulation (mV/min) and lumen volume (mV) (volume of fluorescently labeled LDL solution) were determined. Perfusion with ETS-plasma increased the rate of LDL accumulation (mean +/- SEM, 6.9 +/- 1.8 mV/min) compared with control (1.6 +/- 0.40 mV/min, P < or = .02). LDL accumulation was primarily dependent on LDL interaction with ETS-plasma rather than the interaction of ETS-plasma with the artery wall. Also, ETS-plasma significantly increased lumen volume (43.3 +/- 5.1 mV) compared with control (35.1 +/- 4.4 mV, P < or = .005).


CONCLUSIONS
Exposure to ETS acutely increased LDL accumulation in perfused arteries. Repeated exposure to ETS may represent important early events in atherogenesis.
Department of Internal Medicine, University of California, Davis 95616, USA.
K A
KA
Roberts
A A
AA
Rezai
K E
KE
Pinkerton
J C
JC
Rutledge
D000818:Animals; D002339:Carotid Arteries / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000187:drug effects* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009538:Nicotine / Q000009:adverse effects; D009589:Nitrogen Oxides / Q000009:adverse effects; D010477:Perfusion; D010947:Plants, Toxic; D010949:Plasma; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D014026:Nicotiana; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014664:Vasodilation / Q000187:drug effects
D008078:Cholesterol, LDL; D009589:Nitrogen Oxides; D014028:Tobacco Smoke Pollution; D009538:Nicotine