Immunocytochemical localization of 60-kDa heat shock protein in Vibrio cholerae.
The immunocytochemical localization of the 60-kDa heat shock protein (HSP) in Vibrio cholerae strain 569B was studied by transmission electron microscopy using a combination with the antigen-specific monoclonal antibody (5C3) and immunogold labelling. The labelling with gold particles in V. cholerae detected 2 types; the gold particles were exclusively detected in the cytoplasm for one type and in the periplasmic space for another type, suggesting that the 60-kDa HSP of V. cholerae corresponding immunologically to Escherichia coli GroEL may translocate from the cytoplasm to the periplasmic space in the V. cholerae cell.
Taguchi
H
H
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
Yamaguchi
H
H
Yamamoto
T
T
Kamiya
S
S
D000818:Animals; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D014734:Vibrio cholerae / Q000378:metabolism*
D001426:Bacterial Proteins; D018834:Chaperonin 60