Modified nucleotides as substrates and inhibitors of adenylate kinase from different sources.
The substrate and inhibitory properties of modified nucleotides with respect to adenylate kinase from rabbit muscles, human placenta and Escherichia coli were studied. A number of 5'-hydrogenphosphonates and 5'-fluorophosphates of modified nucleotides were shown to inhibit the phosphorylation reaction catalyzed by these enzymes. A clear difference between phosphonates of 3'-deoxyribonucleotides and the corresponding ribo- and 2',3'-dideoxyribonucleotides was found. 3'-Azido-2',3'-dideoxythymidine and its phosphorus derivatives did not inhibit the adenylate kinase reaction.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
J S
JS
Skoblov
M D
MD
Frank-Kamenetskaya
D N
DN
Chernov
A A
AA
Krayevsky
D000263:Adenylate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D006801:Humans; D007700:Kinetics; D018482:Muscle, Skeletal / Q000201:enzymology; D009711:Nucleotides / Q000378:metabolism* / Q000494:pharmacology*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011817:Rabbits; D013379:Substrate Specificity; D015215:Zidovudine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D009711:Nucleotides; D015215:Zidovudine; D000263:Adenylate Kinase