Application of gels of 0.5 mm thickness to electrophoresis in the automated HPGE-1000 apparatus: improved resolution.
Gel electrophoresis in commercial automated apparatus (HPGE-1000, LabIntelligence, Menlo Park, CA) is conventially conducted in gels of 3 mm thickness at about 15 V/cm. Since the intermittent scanning of the gel allows one to measure band width as a function of migration time, resolution may be evaluated quantitatively. Comparing the value of resolution between two proteins in electrophoresis on agarose gels of 0.5 and 3.0 mm thickness and at various field strengths, it was found that within the given Joule heat dissipation capacity of the apparatus, resolution between the proteins is improved when the gel thickness is reduced from 3.0 to 0.5 mm, which allows for an increase in field strength from 15 V/cm, conventional for that apparatus, to 45 V/cm.
Chang
H T
HT
Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Chrambach
A
A
D001322:Autoanalysis / Q000295:instrumentation*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation*; D005782:Gels; D016014:Linear Models; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results
D005782:Gels; D011506:Proteins