Abnormalities of CD45 isoform expression in HIV infection.
Using monoclonal antibodies against CD45, CD45RA, CD45RB, and CD45RO, the surface density of expression and proportions of different CD45 isoforms were analyzed on CD4+ and CD8+ T lymphocyte subsets in HIV+ patients and controls. Analysis of the relative specific fluorescence (RSF) of CD45 isoforms showed a significant reduction in expression of total CD45 and its isoforms, CD45RA, CD45RO, and CD45RB, on CD4+ and CD8+ lymphocyte subsets in HIV+ patients compared with controls. However, expression of phosphotyrosine in the cytoplasm of CD4+ and CD8+ lymphocytes did not differ significantly between HIV+ patients and controls. Percentages of "naive" CD45RA+RO- CD4+ and CD8+ lymphocytes were lower than control values in all HIV+ patients (P < 0.001). Compared with controls, HIV+ patients had similar percentages of "memory" CD45RA-RO+ CD4+ lymphocytes but significantly higher percentages of memory CD8+ lymphocytes (P < 0.001). Percentages of memory CD8+ lymphocytes were lowest in patients with AIDS (P < 0.001). The consistent reduction in surface expression of all CD45 isoforms observed in our study may be of relevance to the impaired T cell activation characteristic of HIV infection. Also, our results argue against the selective depletion of memory CD4+ lymphocytes in HIV-induced disease.
Department of Immunology, King's College Hospital, London, United Kingdom.
M
M
Mahalingam
A
A
Pozniak
T J
TJ
McManus
G
G
Senaldi
D
D
Vergani
M
M
Peakman
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D005455:Fluorescent Antibody Technique; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D019000:Phosphotyrosine / Q000032:analysis
D019000:Phosphotyrosine; D017493:Leukocyte Common Antigens