NotI jumping and linking clones as a tool for genome mapping and analysis of chromosome rearrangements in different tumors.
Long-range restriction site maps are of central importance for mapping the human genome. The use of clones from linking and jumping libraries for genome mapping offers a promising alternative to the laborious procedures used up until now. In the present review, this research field is analyzed with particular emphasis on the implementation of a shot-gun sequencing strategy for genome mapping and the use of NotI linking clones for analysis of rearrangements in tumors and tumor cell lines.
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
E R
ER
Zabarovsky
V I
VI
Kashuba
R Z
RZ
Gizatullin
G
G
Winberg
V I
VI
Zabarovska
R
R
Erlandsson
D A
DA
Domninsky
V M
VM
Bannikov
E
E
Pokrovskaya
I
I
Kholodnyuk
N
N
Petrov
V M
VM
Zakharyev
L L
LL
Kisselev
G
G
Klein
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D015321:Gene Rearrangement; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases