Isolation and characterization of a bovine gene encoding phenol sulfotransferase.
Phenol sulfotransferases (PST) esterify hydroxylated aromatic compounds with sulfate, and therefore play a role in the metabolism of xenobiotics. In this investigation, a bovine PST gene of 2372 bp was amplified from genomic DNA. Sequence overlap with the cognate cDNA revealed seven exons, with all introns containing GT/AG splicing donor/acceptor site. This product was subcloned into an SV40-based expression vector and transfected into WI-26 human lung fibroblasts. Immunoblot analyses revealed production of the anticipated 32-kDa protein, and the active enzyme displayed steady-state kinetic properties consistent with the enzyme characterized in bovine lung (apparent Km = 6.5 microM for 2-naphthol).
Henry
T
T
Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68198-5300, USA.
Kliewer
B
B
Palmatier
R
R
Ulphani
J S
JS
Beckmann
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D015239:Arylsulfotransferase / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005091:Exons; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D015151:Immunoblotting; D007438:Introns; D008969:Molecular Sequence Data; D014162:Transfection
D004274:DNA, Recombinant; D015239:Arylsulfotransferase