Automated capillary electrochromatography: reliability and reproducibility studies.
The routine application of capillary electrochromatography (CEC) is demonstrated by incorporating 75 microns I.D. capillaries packed with 3 microns octadecylsilica (ODS) particles into a commercial CZE instrument. A mixture of several neutral compounds is separated into its components with an average efficiency up to 181 000 plates/m in less than 8 min. Hundreds of consecutive runs are performed over a period of weeks from which it is concluded that the reproducibility of the capacity factors is better than 2% and that CEC separations can be achieved in a reliable and routine manner.
Department of Chemistry, Stanford University, CA 94305, USA.
M T
MT
Dulay
C
C
Yan
D J
DJ
Rakestraw
R N
RN
Zare
D002845:Chromatography / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results