Degradation of distinct assembly forms of immunoglobulin M occurs in multiple sites in permeabilized B cells.
Protein degradation is essential for quality control which retains and eliminates abnormal, unfolded, or partially assembled subunits of oligomeric proteins. The localization of this nonlysosomal pre-Golgi degradation to the endoplasmic reticulum (ER) has been mostly deduced from kinetic studies and carbohydrate analyses, while direct evidence for degradation within the ER has been provided by in vitro reconstitution of this process. In this article, we took advantage of the transport incompetence of permeabilized cells to directly demonstrate that the selective degradation of secretory IgM (sIgM) in B lymphocytes is transport-dependent. We show that, upon permeabilization of the plasma membrane with either streptolysin O or digitonin, sIgM is not degraded unless transport is allowed. Nevertheless, upon complete reduction of interchain disulfide bonds with thiols, the free mu heavy chains are degraded by a transport-independent quality control mechanism within the ER. This latter degradation is nonselective to the secretory heavy chain mus, and the membrane heavy chain mum, which is normally displayed on the surface of the B cell, is also eliminated. Moreover, the degradation of free mus is no longer restricted to B lymphocytes, and it takes place also in the ER of plasma cells which normally secrete polymers of sIgM. Conversely, when assembled with the light chain, the degradation is selective to sIgM, is restricted to B lymphocytes, and is a transport-dependent post-ER event.
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
D
D
Winitz
I
I
Shachar
Y
Y
Elkabetz
R
R
Amitay
M
M
Samuelov
S
S
Bar-Nun
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins; D020126:Brefeldin A; D002460:Cell Line; D002463:Cell Membrane Permeability; D003517:Cyclopentanes / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism; D004072:Digitonin; D004229:Dithiothreitol / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006825:Hybridomas; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013301:Streptolysins; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology
D001426:Bacterial Proteins; D003517:Cyclopentanes; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D011500:Protein Synthesis Inhibitors; D013301:Streptolysins; D013438:Sulfhydryl Compounds; D013450:Sulfones; C025964:streptolysin O; D020126:Brefeldin A; C095300:bis(2-mercaptoethyl)sulfone; D008715:Methionine; D003545:Cysteine; D004072:Digitonin; D004229:Dithiothreitol