Fat infiltration in liver of rats induced by different dietary plant oils: high oleic-, medium oleic- and high petroselinic acid-oils.
Beneficial effects on health parameters by ingestion of plant oils having high levels of monounsaturated fatty acids such as oleic (cis-9-octadecenoic) acid have been described in several studies. Recently, we have shown that feeding of coriander oil containing high proportions of a positional isomer of oleic acid, i.e. petroselinic (cis-6-octadecenoic) acid, led to significantly decreasing proportions of arachidonic (all cis-5,8,11,14-eicosatetraenoic) acid in the cellular lipids. Here, we report histopathological findings in male Wistar rats fed isocaloric diets containing 2% corn oil and 12% (w/w), each, of different plant oils (high-oleic and conventional sunflower oil, olive oil, rapeseed oil as well as coriander oil) containing varying levels of monounsaturated fatty acids, i.e. oleic or petroselinic acids. One group of animals was fed a standard diet containing 4% (w/w) of fat as control. Liver, heart, aorta, stomach, and spleen were anatomically and histologically examined. Livers of animals from all the groups that had received high-fat diets-except the coriander oil group-showed moderate non-degenerative fat infiltrations of the hepatocytes beginning in the periportal fields. Marked to severe fat infiltration was observed in hepatocytes of animals fed coriander oil. In this experimental group the livers were found to contain fatty cysts in addition to hepatocytes with mixed-size lipid vesicles. Moreover, enlarged nuclei were observed in numerous hepatocytes without fat infiltration. Fat infiltration was not observed in livers of animals fed a rat standard diet. In the cells of all other organs and tissues studied, in particular heart and aorta, neither non-degenerative fat infiltrations nor other degenerative changes were observed for any group. Our results show that feeding of fat-rich diets containing varying proportions of C18 cis-monoenoic fatty acids induced different histopathological alterations in the livers of rats, depending on position of the double bond. Plant oils containing varying proportions of oleic acid lead exclusively to moderate fat infiltration in the hepatocytes, whereas in the animals fed high-petroselinic coriander oil degenerative alterations (fatty cysts) as well as -in several cases-enlarged nuclei were found that are likely due to a non-specific stimulation of the hepatocytes resulting from the cell degeneration observed.
Richter
K D
KD
Zentrale Tierexperimentelle Einrichtung, Medizinischen Fakultät Westfalische Wilhelms-Universität Münster.
Mukherjee
K D
KD
Weber
N
N
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000009:adverse effects*; D005234:Fatty Liver / Q000139:chemically induced*; D007536:Isomerism; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D019301:Oleic Acid / Q000009:adverse effects; D009829:Oleic Acids / Q000009:adverse effects; D010938:Plant Oils / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar
D004042:Dietary Fats, Unsaturated; D009829:Oleic Acids; D010938:Plant Oils; D019301:Oleic Acid; C008820:petroselinic acid