The oxygen-binding intermediates of human hemoglobin: evaluation of their contributions to cooperativity using zinc-containing hybrids.
Hemoglobin tetramers [Zn/FeO(2)] containing oxygenated subunits (FeO(2)), in combination with unligated subunits containing zinc-substituted hemes (Zn), were analyzed to determine their contributions to the cooperativity of oxygen binding at the Fe sites. Energetic consequences of possible perturbation by zinc substitution were evaluated in all combinations of unligated Zn/Fe hybrid tetramers. A general thermodynamic strategy that corrects for the energetic effects of substituting a second metal for Fe showed the perturbations of Zn substitution to be negligible. This permitted cooperativity parameters of the native Fe/FeO(2) intermediates to be calculated from data on the corresponding Zn/FeO(2) molecules. These parameters, determined explicitly for all eight oxygen-binding intermediates (Fe/FeO(2)), were found to be identical to those predicted earlier from analyzing the O(2) binding data of normal hemoglobin according to the "molecular code" of hemoglobin allostery. The cooperativity parameters determined for this system showed the same distribution pattern found previously for five other oxygen analog systems (Fe/FeCN, FE/Mn(3+), Co/FECO, Co/FeCN, and Fe/FeCO).
Huang
Y
Y
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Doyle
M L
ML
Ackers
G K
GK
D019281:Dimerization; D006451:Hemoglobin, Sickle / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007501:Iron; D007700:Kinetics; D046911:Macromolecular Substances; D008956:Models, Chemical; D008961:Models, Structural; D010100:Oxygen / Q000097:blood; D010108:Oxyhemoglobins / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D007501:Iron; D010100:Oxygen