Differences in heavy-ion-induced DNA double-strand breaks in a mouse DNA repair-deficient mutant cell line (SL3-147) before and after chromatin proteolysis.
DNA double-strand breaks induced by X- or neon beam-irradiation in a DNA double-strand break-repair-deficient mutant cell line (SL3-147) were examined. The increase in the number of DNA double-strand breaks was dose depend after irradiation with X-rays and neon beams and was enhanced by chromatin-proteolysis treatment before irradiation. These results suggest that the induction of DNA double-strand breaks by ionizing radiation, including heavy-ions, is influenced by the chromatin structure.
Murakami
M
M
Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences, Chiba-shi, Japan.
Eguchi-Kasai
K
K
Sato
K
K
Minohara
S
S
Yatagai
F
F
Kanai
T
T
D000818:Animals; D002843:Chromatin / Q000502:physiology*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D016521:Electrophoresis, Gel, Pulsed-Field; D051379:Mice
D002843:Chromatin; D004247:DNA