Modeling spiral Ca2+ waves in single cardiac cells: role of the spatial heterogeneity created by the nucleus.
Excitation-contraction coupling in cardiomyocytes is known to rely on the Ca(2+)-induced Ca2+ release mechanism. This autoamplification process is also very apparent when voltage-clamped or Ca(2+)-overloaded myocytes exhibit fast-propagating Ca2+ waves. Although most of the fronts are planar, some adopt a spiral shape, revealing additional characteristics about the excitability and structure of the cardiac cell (P. Lipp and E. Niggli, Biophys. J. 65: 2272-2276, 1993: J. Engel, M. Fechner, A. Sowerby, S. Finch, and A. Stier, Biophys. J. 66: 1756-1762, 1994). Using a previously developed model for Ca2+ oscillations and waves (A. Goldbeter, G. Dupont, and M.J. Berridge, Proc. Natl. Acad. Sci. USA 87: 1461-1465, 1990; G. Dupont and A. Goldbeter, Biophys. J. 67: 2191-2204, 1994), we study by numerical simulations different conditions in which spiral Ca2+ waves can occur as a result of the spatial heterogeneity created by the nucleus in a system with geometry resembling that of a myocyte. A region of the cell lacking Ca2+ pools, acting as an obstacle able to break the propagation of planar waves, suffices to initiate a spiral wave; however, this region must be properly placed with respect to the pacemaker. An obstacle behaving as a barrier to diffusion is also able to create the initial bending that can lead to the spiral wave. We study how the occurrence of spiral Ca2+ waves in single cardiomyocytes is influenced by factors such as the stimulus location and the position, shape, and dimensions of the obstacle to planar wave propagation.
Unité de Chronobiologie Théorique, Faculté des Sciences, Université Libre de Bruxelles, Belgium.
G
G
Dupont
J
J
Pontes
A
A
Goldbeter
D002118:Calcium / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D003198:Computer Simulation; D004058:Diffusion; D008954:Models, Biological; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010507:Periodicity
D002118:Calcium