Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens.
Poliovirus vectors are being studied as potential vaccine delivery systems, with foreign genetic sequences incorporated as part of the viral genome. The foreign sequences are expressed as part of the viral polyprotein. Addition of proteolytic cleavage sites at the junction of the foreign polypeptide and the viral proteins results in cleavage during polyprotein processing. The ability of foot-and-mouth disease virus (FMDV) 2A to mediate proteolytic cleavage in the context of poliovirus vectors was studied. The results demonstrate that FMDV 2A is able to generate cleavage of the foreign antigen from the viral polyprotein. A second cleavage event between the FMDV 2A peptide and the foreign protein was also observed.
Mattion
N M
NM
Wyeth-Lederle Vaccines and Pediatrics, Viral Vaccine Research, Pearl River, New York 10965, USA. nmattion@virol.wamani.apc.org.
Harnish
E C
EC
Crowley
J C
JC
Reilly
P A
PA
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D005537:Aphthovirus / Q000201:enzymology* / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011055:Poliovirus Vaccine, Oral / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins
D000956:Antigens, Viral; D011055:Poliovirus Vaccine, Oral; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C040570:glycoprotein D-herpes simplex virus type 2; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus