Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation.
Phenotype of P815 mouse mast cells changes markedly during culture in the peritoneal cavity of syngenic BDF1 mice. The cells, cultured for 1 week in the peritoneal cavity of syngenic BDF1 mice, proliferate and express high levels of L-histidine decarboxylase (HDC) and mouse mast cell protease (MMCP)-6 mRNAs, indicating the ability of P815 cells to differentiate toward mature connective tissue mast cells. Peritoneal fluid aspirated from P815-inoculated BDF1 mouse and added to cultured P815 cells in vitro was also found to induce HDC mRNA expression, suggesting that at least some of the humoral factors in the peritoneal fluid induce HDC mRNA transcription. Among the erythroid transcription factors, P815 cells expressed GATA-2 but not GATA-1 mRNA before and after the intraperitoneal incubation. In contrast, the expression of NF-E2 subunit p45 disappeared, while expression of subunit mafK was markedly reduced after incubation. Cotransfection assays using HDC-luciferase reporter and p45 and/or mafK expression constructs showed that NF-E2 affects the transactivation of HDC gene. These results suggest that NF-E2 is also an important transcription factor in mast cell differentiation.
Department of Pharmacology I, Tohoku University School of Medicine, Sendai 980-77, Japan. J23613@CCTU.CC.TOHOKU.AC.JP
H
H
Ohtsu
A
A
Kuramasu
S
S
Suzuki
K
K
Igarashi
Y
Y
Ohuchi
M
M
Sato
S
S
Tanaka
S
S
Nakagawa
K
K
Shirato
M
M
Yamamoto
A
A
Ichikawa
T
T
Watanabe
D000818:Animals; D002460:Cell Line; D017931:DNA Primers / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006632:Histamine / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D017403:In Situ Hybridization; D016350:Leucine Zippers; D050985:MafK Transcription Factor; D008407:Mast Cells / Q000201:enzymology*; D051379:Mice; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D009687:Nuclear Proteins / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D053802:Tryptases; D016335:Zinc Fingers
D017931:DNA Primers; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; C494689:Gata1 protein, mouse; C494712:Gata2 protein, mouse; D050985:MafK Transcription Factor; C494696:Mafk protein, mouse; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; C494693:Nfe2 protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; C509190:Tpsb2 protein, mouse; D014157:Transcription Factors; D006632:Histamine; D012697:Serine Endopeptidases; C509222:Tpsab1 protein, mouse; D053802:Tryptases; D006640:Histidine Decarboxylase