Gestational changes of glucose transporter gene expression in the mouse placenta and decidua.
Glucose is required for fetal development and energy metabolism. The glucose transfer from maternal circulation to fetus, in which glucose transporter (GLUT) should play an important role, is fundamental in the utero-placental-fetal system. In this study, the gestational changes of GLUT1 and GLUT3 mRNA level in the mouse placenta and decidua were analyzed by Northern blot analysis. The levels of GLUT1 mRNA in the mouse placenta and decidua increased as gestational day proceeded. Although the level of GLUT3 mRNA in the decidua slightly decreased as pregnancy proceeded, there was a switch in size of a major band of GLUT3 from 4.1 kb to 2.7 kb in the mouse placenta detected by Northern blot analysis. These findings suggest the presence of a difference in the gestational modulation of the level of GLUT1 and GLUT3 in the uteroplacental system.
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
M
M
Yamaguchi
M
M
Sakata
K
K
Ogura
A
A
Miyake
D000818:Animals; D015152:Blotting, Northern; D003656:Decidua / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005865:Gestational Age; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051379:Mice; D008813:Mice, Inbred ICR; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D011257:Pregnancy Proteins; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse