Impairment of antioxidants in colonic epithelial cells isolated from trinitrobenzene sulphonic acid-induced colitis rats. Protective effect of rebamipide.
BACKGROUND
The functional status of glutathione, its related enzymes, and Cu,Zn-superoxide dismutase in colonocytes isolated from rats with trinitrobenzene sulphonic acid-induced colitis, and the effect of rebamipide (a scavenger) on these antioxidants and on colitis, were studied.


METHODS
The rats were treated with rebamipide, killed on day 14, and compared with controls. Glutathione and enzymatic activities were spectrophotometrically estimated. The effect of rebamipide on colitis was also assessed histologically and by myeloperoxidase activity.


RESULTS
In controls the concentration of glutathione and the activities of glutathione S-transferase and Cu,Zn-superoxide dismutase were decreased, but that of glutathione peroxidase was increased. Rebamipide prevented the impairment of these enzymes, restored the concentration of glutathione, and attenuated (low damage scores and myeloperoxidase activity) the severity of the colitis.


CONCLUSIONS
These features suggest that the impairment of antioxidants is closely related to the development of the inflammatory lesions, and rebamipide has a beneficial effect on experimental colitis.
Dept. of Pathology, Nagasaki University School of Medicine, Japan.
W L
WL
Zea-Iriarte
K
K
Makiyama
S
S
Goto
K
K
Murase
Y
Y
Urata
I
I
Sekine
K
K
Hara
T
T
Kondo
D000409:Alanine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology*; D046508:Culture Techniques; D004195:Disease Models, Animal; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D015363:Quinolones / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D015363:Quinolones; D014302:Trinitrobenzenesulfonic Acid; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione; C052785:rebamipide; D000409:Alanine