H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells.
Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N-vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.
Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
A V
AV
Bogachev
R A
RA
Murtazina
V P
VP
Skulachev
D000693:Anaerobiosis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D042967:Electron Transport Complex I; D004583:Electrons; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D007506:Iron-Sulfur Proteins; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen; D011522:Protons
D007506:Iron-Sulfur Proteins; D011522:Protons; D010088:Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; C045664:dimethyl sulfoxide reductase; D042967:Electron Transport Complex I; D010100:Oxygen; D004121:Dimethyl Sulfoxide