Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo.
Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 x 10(3) Mr form (ADD-95) and an 87 x 10(3) Mr form (ADD-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
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Lipshitz
D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D005260:Female; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis; D006080:Ovarian Follicle / Q000378:metabolism / Q000502:physiology; D013049:Spectrin / Q000378:metabolism; D000099291:Adducins
D000199:Actins; D002148:Calmodulin-Binding Proteins; D013049:Spectrin; D000099291:Adducins