A radioimmunoassay for the determination of tipredane in human plasma.
A sensitive method for the determination of tipredane in human plasma has been developed. Prior to radioimmunoassay, proteins are removed from plasma by precipitation with acetonitrile. A [125I]iodohistamide derivative of tipredane is used as a heterogeneous radioligand. The primary antiserum is raised in sheep against a tipredane (O-carboxymethyl) oxime-bovine serum albumin conjugate. Donkey anti-sheep IgG antiserum is used as the secondary antiserum in a double antibody separation procedure. The limit of quantification of the method is 1 ng ml-1 for 500 microliters of plasma. There is no significant interference from either established or putative metabolites of tipredane, endogenous steroids or a wide range of other drugs. The assay was used to determine the concentrations of tipredane in plasma samples collected in pilot clinical studies.
Bayliss
M A
MA
Fisons plc, Department of Physical Chemistry, Loughborough, Leicestershire, UK.
Preston
J R
JR
Wilkinson
D
D
Wilkinson
D J
DJ
Lockley
W J
WJ
D000287:Administration, Topical; D000730:Androstadienes / Q000097:blood* / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000378:metabolism; D005938:Glucocorticoids; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D011863:Radioimmunoassay / Q000379:methods*
D000730:Androstadienes; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D007106:Immune Sera; C051756:tipredane