Aluminum potentiates P(i) transport stimulation induced by fluoride in osteoblast-like cells.
The effect of aluminum (AI) on inorganic phosphate (P(i)) transport stimulation induced by fluoride (F) was investigated in MC3T3-E1 osteoblast-like cells. Al potentiated the increase in P(i) transport activity induced by F in a dose- and time-dependent manner. Results obtained with deferoxamine mesylate, an Al chelator, suggest that a fluoroalumino complex is probably the active F molecule responsible for the change in P(i) transport observed in this study. The signaling pathway responsible for the stimulation of P(i) transport by F+Al likely involves a tyrosine phosphorylation process but neither a protein kinase C nor a mitogen-activated protein kinase pathway. As previously found in UMR-106 cells for F alone, F+Al potentiated the change in P(i) transport induced by fetal calf serum. A similar interaction was found between F+Al and thrombin acting through a G protein-coupled receptor. These observations are compatible with the hypothesis that F+Al could interact with G protein-coupled receptors associated with a signaling tyrosine phosphorylation process involved in the regulation of P(i), transport in osteoblast-like cells.
Department of Medicine, University Hospital of Geneva, Switzerland.
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Imai
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Burgener
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Zhen
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Benjour
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Caverzasio
D016475:3T3 Cells; D000535:Aluminum / Q000008:administration & dosage*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D015232:Dinoprostone / Q000502:physiology; D004357:Drug Synergism; D005459:Fluorides / Q000008:administration & dosage*; D006133:Growth Substances / Q000502:physiology; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D019000:Phosphotyrosine / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology; D015398:Signal Transduction
D006133:Growth Substances; D010710:Phosphates; D019000:Phosphotyrosine; D000535:Aluminum; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D015232:Dinoprostone; D005459:Fluorides