Factor XSt. Louis II. Identification of a glycine substitution at residue 7 and characterization of the recombinant protein.
A molecular defect in factor X (fX) results from a point mutation that causes glycine substitution for gamma-carboxylated glutamic acid at position 7. The variant (fXSt. Louis II) and wild type (fXWT) proteins were produced in a mammalian expression system and characterized. fXSt. Louis II has <1% and approximately 3% of normal clotting activity in modified prothrombin time and partial thromboplastin time assays, respectively. The rate of activation of fXSt. Louis II by factor VIIa and tissue factor is undetectable under conditions that result in complete activation of fXWT; activation by factors VIIIa and IXa is approximately 30% of normal activation. The X-activating protein from Russell's viper venom activates fXSt. Louis II completely but at a reduced rate. Thrombin generation on phoshopolipid vesicles or activated platelets is approximately 30% or approximately 5%, respectively. Membrane-dependent autolysis is markedly reduced for fXSt. Louis II. In reactions that are not surface-dependent, fXSt. Louis II is nearly identical to that of fXWT. The rate of inhibition by antithrombin is indistiguishable, as is the rate of thrombin formation in the absence of phospholipid, with or without factor Va.
Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. miletich@labmed.wustl.edu
A E
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Rudolph
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Mullane
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Porche-Sorbet
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Tsuda
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JP
Miletich
D000990:Antithrombin III / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005170:Factor X / Q000737:chemistry* / Q000235:genetics; D005998:Glycine; D006801:Humans; D007700:Kinetics; D017354:Point Mutation; D011516:Prothrombin / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry*
D011994:Recombinant Proteins; C102363:factor X St. Louis II; D000990:Antithrombin III; D011516:Prothrombin; D005170:Factor X; D005998:Glycine