Expression of biglycan, decorin and fibromodulin in the hypertrophic phase of experimental osteoarthritis.
This study sought to assess the relative levels of the mRNAs of the core proteins of the small proteoglycans (PGs) biglycan, decorin and fibromodulin in the hypertrophic phase of the early osteoarthritis (OA) that follows joint injury. Experimental OA was induced in eight dogs by transection of the anterior cruciate ligament. Articular cartilage was harvested from each joint, the total RNA was extracted and the concentration of DNA in the cartilage was measured. The relative levels of mRNA for biglycan, decorin and fibromodulin were assessed by northern blot analyses. An increase in cartilage mass with no increase in DNA concentration confirmed that the joints were in the hypertrophic phase that follows joint injury. The total RNA per microgram of DNA was increased 2.5 times. Compared with control cartilage, the mRNA levels in osteoarthritic cartilage, when normalized to the concentration of DNA, were increased 3.9 times for biglycan, 1.2 times for decorin and 2.4 times for fibromodulin. Because these small PGs affect collagen fibrillogenesis in vitro, their discoordinate metabolism may contribute to the abnormal collagen formation and deposition that occurs in OA and to the ultimate failure of the articular cartilage.
Dourado
G S
GS
McCaig Center for Joint Injury and Arthritis Research, Calgary, Alberta, Canada.
Adams
M E
ME
Matyas
J R
JR
Huang
D
D
D000818:Animals; D058578:Biglycan; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000032:analysis; D058575:Decorin; D004195:Disease Models, Animal; D004285:Dogs; D016326:Extracellular Matrix Proteins; D000071235:Fibromodulin; D006984:Hypertrophy; D008297:Male; D015345:Oligonucleotide Probes / Q000737:chemistry; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012044:Regression Analysis
D058578:Biglycan; D002352:Carrier Proteins; D058575:Decorin; D016326:Extracellular Matrix Proteins; D015345:Oligonucleotide Probes; D011509:Proteoglycans; D012333:RNA, Messenger; D000071235:Fibromodulin; D004247:DNA