Real time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Heid
C A
CA
BioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USA.
Stevens
J
J
Livak
K J
KJ
Williams
P M
PM
D002460:Cell Line; D018076:DNA, Complementary; D005169:Factor VIII / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014162:Transfection
D018076:DNA, Complementary; D005169:Factor VIII