Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis.
Effective distinction was achieved among a wide range of lupin grain samples by either sodium dodecyl sulphate (SDS)-gel electrophoresis or capillary electrophoresis, based on grain-protein composition. Capillary electrophoresis was faster (< 1 h) and provided slightly greater distinction between the samples. On the other hand, SDS-gel electrophoresis could provide a greater through-put of samples in a 24 h period. Either technique could be used successfully to distinguish between lupin species and cultivars for taxonomic analysis or seed identification.
Pollard
N J
NJ
CSIRO Grain Quality Research Laboratory, Division of Plant Industry, North Ryde, Australia. naomi.pollard@pi.csiro.au
Wrigley
C W
CW
Bekes
F
F
Aumatell
A
A
MacRitchie
F
F
D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000235:genetics*; D005838:Genotype; D008832:Microchemistry; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal; D012680:Sensitivity and Specificity; D013045:Species Specificity
D010940:Plant Proteins