A numerical study of persistence length effects on DNA conformation in sequencing electrophoresis.
We have developed a program to evaluate the influence of DNA stiffness on molecular mobility and conformation during electrophoresis. This (currently) two-dimensional numerical study models DNA as a chain of uniformly charged beads connected to one another by elastic segments, of finite mean size, in the presence of fixed obstacles representing gel fibers. Contrary to the standard biased reptation model (BRM), our Langevin-type dynamics for the chain are microscopic and warrant studies of fine effects such as inner chain orientation. Using this model, we show that the introduction of a persistence length decreases the (saturated) mobility at high electric fields, providing strong evidence that the gel generates a friction force and not only a (dissipation-free) constraint force. We also show that the persistence length leads to an increase of the chain orientation in the field direction. This suggests that DNA stiffness causes the saturation plateau value to be reached for smaller chain sizes than those predicted by the BRM model.
Division de Recherche Expérimentale, Institut de Physique Nucleaire, Orsay, France.
E
E
Guerry
O C
OC
Martin
H
H
Tricoire
R
R
Siebert
L
L
Valentin
D004247:DNA / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA
D004247:DNA