Molecular analysis and polymorphism of the DLA-DQA gene.
A full-length cDNA clone and two overlapping genomic clones corresponding to the canine DQA class II gene were isolated and sequenced. Restriction mapping and sequence data allow identification and orientation of the five exons corresponding to the alpha (alpha) chain. Sequence analysis of exon 2 amplified from 17 unrelated dogs of various breeds identified seven alleles. The structure of the canine DQA gene is similar to HLA-DQA1 and other mammalian DQA genes. This study will serve as a reference for developing a typing system for the DLA-DQA gene for donor and recipient matching in the canine model for organ and bone marrow transplantation.
Transplantation Biology Program, Fred Hutchinson Cancer Research Center, Seattle, USA.
J L
JL
Wagner
R C
RC
Burnett
S A
SA
DeRose
R
R
Storb
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004285:Dogs; D015723:Gene Library; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000276:immunology*
D018076:DNA, Complementary; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D015395:Histocompatibility Antigens Class I; C039692:histocompatibility antigen DLA