Molecular cloning of a gene from 'chromosome 3 specific' genomic library that encodes a novel CC-chemokine receptor like isotype.
CC-chemokine receptors form a closely linked locus in the p21-23 region on the short arm of human chromosome 3. We report the isolation of a novel chemokine receptor like gene from a 'human chromosome 3 specific' genomic library, screened by cross-hybridization with cDNA probes derived from known chemokine receptors. The gene, CKR-SKG2 contains an open reading frame that encodes a 359-377 amino acid protein, with two potential in frame start codons. However, comparative Southern blot analysis of human, mouse and hamster genomic DNA, revealed that CKR-SKG2 is the hamster ortholog of a novel chemokine receptor. This presumably occurred because chromosome 3 used for construction of the genomic library were purified from a human-hamster somatic cell hybrid. Further studies are underway to identify and clone the putative human homolog of this novel chemokine receptor.
SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19444, USA. Shalley_K_Gupta@SBPHRD.Com@INET
S K
SK
Gupta
M
M
Naso
J M
JM
Stadel
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D015342:DNA Probes; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine