Expression of CD44 splice variants in metastatic and non-metastatic mouse tumour cell lines.
Different splice variants of the CD44 cell-surface molecule have been linked to metastasis formation in several animal and human cancers. We have used metastatic CSML-100 and non-metastatic CSML-0 mouse adenocarcinoma cell lines to determine whether variant CD44 molecules could be implicated in the different behaviour of these cells. Two CD44 splice variants containing exons v7-v10 and v8-v10 were detected in the non-metastatic CSML-0. Two other mouse cell lines, the normal mammary gland NMuMG and the mammary pre-neoplastic CL-S1 were also found to express these exons. A short (hematopoietic) CD44 isoform was expressed in the metastatic CSML-100 and three other mouse mammary tumour cell lines. Overexpression of v7-v10 and v8-v10 CD44 variants in CSML 100 cells did not decrease their metastatic potential.
Tkach
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International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
Li
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Bestagno
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Burrone
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D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000556:secondary; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D018960:Hyaluronan Receptors