Neuroblastoma cell culture assay shows that Carcinoscorpius rotundicauda haemolymph neutralizes tetrodotoxin.
Veratridine facilitates the influx of Na+ into Neuro-2A cells and this process is exacerbated by the presence of a Na+/K(+)-ATPase inhibitor, ouabain, leading to a reduction in cell viability. However, tetrodotoxin neutralizes the cytotoxic effects of veratridine, hence sustaining the viability of Neuro-2A cells. This neutralizing ability was negated when TTX was first reacted with cell-free haemolymph of Carcinoscorpius rotundicauda, resulting in reduced cell viability. These results therefore indicate a bona fide effect of the cell-free haemolymph against tetrodotoxin as demonstrated by in vitro cell culture technique.
Yeo
D S
DS
Department of Microbiology, National University of Singapore, Republic of Singapore.
Ding
J L
JL
Ho
B
B
D000818:Animals; D003386:Brachyura; D002470:Cell Survival / Q000187:drug effects; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology*; D010042:Ouabain / Q000633:toxicity*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013779:Tetrodotoxin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014701:Veratridine / Q000633:toxicity*
D013779:Tetrodotoxin; D010042:Ouabain; D014701:Veratridine; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase