Estimations of average particle sizes and size distributions of commercially available human-insulin aqueous suspensions using laser-light diffraction spectroscopy.
The average diameter and size distribution of particles in commercially available isophane-insulin and zinc-insulin aqueous suspensions (11 products) were examined using laser-light diffraction spectroscopy (LLDS). Wide variation was observed between products in volume-weighted and number-weighted average diameters. The particles in the insulin aqueous suspensions were found to be mono-dispersed within relatively narrow size distributions. Sonication for a short time led to a downshift in the apparent average diameter, which was most remarkable with isophane-insulin aqueous suspensions. This could be ascribed to dispersion of aggregated needle-like crystals and/or crystal breakage. In the case of biphasic isophane-insulin aqueous suspensions, the average size as well as the size distribution shifted toward the smaller end of the scale with decrease in the isophane content.
Komatsu
H
H
Division of Drugs, Osaka Branch, National Institute of Health Sciences, Japan.
Kitajima
A
A
Nakata
Y
Y
Okada
S
S
D006801:Humans; D007336:Insulin, Isophane / Q000737:chemistry*; D049528:Insulin, Long-Acting / Q000737:chemistry*; D007834:Lasers; D009995:Osmosis; D010316:Particle Size; D012542:Scattering, Radiation; D013535:Suspensions
D049528:Insulin, Long-Acting; D013535:Suspensions; D007336:Insulin, Isophane