Aging impairs regulation of intracellular pH in rat hippocampal slices.
We investigated whether aging changed H+ homeostasis in hippocampal slices bathed in HEPES buffer. Intracellular pH in hippocampal slices from rats aged 26-27 months (7.06 +/- 0.02) was significantly lower compared with that in slices from rats aged 6-7 months (7.16 +/- 0.04). Age did not influence extracellular ph. Age-related reductions in intracellular pH may reflect altered pH regulation that potentially affects brain function and could contribute to the increased vulnerability of the aged brain to metabolic stress.
Roberts
E L
EL
Department of Neurology, University of Miami, School of Medicine, FL 33136, USA.
Sick
T J
TJ
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001578:Benzopyrans; D005453:Fluorescence; D006624:Hippocampus / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012235:Rhodamines / Q000378:metabolism
D001578:Benzopyrans; D009284:Naphthols; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D006859:Hydrogen