Evidence from in vitro differentiating cells that adrenoceptor agonists can increase uncoupling protein mRNA level in adipocytes of adult humans: an RT-PCR study.
In vivo data have suggested that adrenergic signals can reactivate dormant brown adipocytes in adult humans. We report here a system based on primary cultures of perirenal adipocytes from human adults and reverse transcription-PCR of uncoupling protein mRNA. Norepinephrine and compounds classified as beta 3-adrenoceptor agonists in rodents increased uncoupling protein mRNA level in human adipocytes (presumably brown adipocytes). Although we did not demonstrate that the observed effect was mediated by beta 3-adrenoceptors, it is proposed that this system could be used to appreciate the ability of beta-adrenoceptor agonists to activate UCP gene transcription and help to select beta 3-adrenoceptor agonists and antagonists prior to in vivo trials; indeed, the difficulty in developing such drugs that are effective in humans may result from the fact that the screening of molecules has historically been made in rodents.
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, Meudon, France.
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Champigny
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Ricquier
D017667:Adipocytes / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000328:Adult; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism; D007473:Ion Channels; D007680:Kidney Neoplasms / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D024101:Mitochondrial Proteins; D010673:Pheochromocytoma / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D022702:Receptors, Adrenergic, beta-3; D013234:Stem Cells / Q000378:metabolism*; D000071256:Uncoupling Protein 1
D000318:Adrenergic beta-Agonists; D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D000071256:Uncoupling Protein 1; D012194:RNA-Directed DNA Polymerase