Identification of cis-acting elements as DNase I hypersensitive sites in lysozyme gene chromatin.
DNase I hypersensitive sites in chromatin of eukaryotic cells mark the positions of multifactorial cis-acting elements. Mapping DH sites by indirect end labeling is a convenient procedure used for identifying regulatory elements within extensive regions of chromatin and for gaining information about their functional specificity as well as their fine structure.
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Germany.
A E
AE
Sippel
H
H
Saueressig
M C
MC
Huber
H C
HC
Hoefer
A
A
Stief
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Borgmeyer
C
C
Bonifer
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002645:Chickens; D002843:Chromatin / Q000235:genetics* / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D003850:Deoxyribonuclease I; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology; D007202:Indicators and Reagents; D009000:Monocytes / Q000166:cytology / Q000201:enzymology; D009113:Muramidase / Q000096:biosynthesis* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping / Q000379:methods; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis
D002843:Chromatin; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D009113:Muramidase; D001616:beta-Galactosidase