The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.
There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS-PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
J E
JE
Wellington
G P
GP
Allen
A A
AA
Gooley
D N
DN
Love
N H
NH
Packer
J X
JX
Yan
J M
JM
Whalley
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005814:Genes, Viral; D006031:Glycosylation; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D006736:Horses; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D009005:Monosaccharides; D014759:Viral Envelope Proteins; C077966:glycoprotein 300, Equid herpesvirus 1