Protein components of a rat brain synaptic junctional protein preparation.
Antisera against a rat brain synaptic protein preparation, the postsynaptic density (PSD) fraction, were used to isolate cDNA clones by expression screening of a rat brain cDNA library. About one fifth of more than 200 analyzed cDNAs encoding potential synapse-associated proteins were previously unknown. Identifiable proteins include, among others, components of the pre- and postsynaptic cytoskeleton, synaptic vesicle proteins and several protein kinases and kinase substrates. This demonstrates that both pre- and postsynaptic elements purify with the PSD fraction.
Langnaese
K
K
Federal Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, Magdeburg, Germany.
Seidenbecher
C
C
Wex
H
H
Seidel
B
B
Hartung
K
K
Appeltauer
U
U
Garner
A
A
Voss
B
B
Mueller
B
B
Garner
C C
CC
Gundelfinger
E D
ED
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D017630:Connexins / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000032:analysis; D018076:DNA, Complementary / Q000302:isolation & purification; D016326:Extracellular Matrix Proteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011494:Protein Kinases / Q000032:analysis; D051381:Rats; D013569:Synapses / Q000737:chemistry*
D017630:Connexins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011494:Protein Kinases