Evaluation of p53 protein expression in renal cell carcinoma: comparison of two antibodies.
The aims of this study were to establish a profile for the expression of p53 in primary renal cell carcinoma using the polyclonal antibody NCL-CB1 and the monoclonal antibody D07, and to compare the results of staining with both antibodies. Ninety-six cases were studied using formalin-fixed paraffin-embedded tissue. Positive nuclear staining ranged from 5% (D07) to 12% (NCL-CM1). Positive cytoplasmic staining ranged from 7% (D07) to 25% (NCL-CM1) of cases. Interobserver agreement was excellent. The findings suggest that such a low level of immunohistochemical positivity reduces any potential prognostic value for p53 in this tumour type.
Department of Pathology, Royal College of Surgeons, Dublin 2, Ireland.
C J
CJ
Walsh
E W
EW
Kay
T
T
Lynch
M
M
Walsh
C
C
Milburn
P A
PA
McLean
D
D
Murphy
M
M
Donovan
M B
MB
Leader
D000906:Antibodies; D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D000906:Antibodies; D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53