Immunocytochemical colocalizations of insulin, aromatic L-amino acid decarboxylase, dopamine beta-hydroxylase, S-100 protein and chromogranin A in B-cells of the chicken endocrine pancreas.
The colocalization of aromatic L-amino acid decarboxylase (AADC), dopamine beta-hydroxylase (DBH), S-100 protein and chromogranin A (CgA) in the insulin-containing B-cells of the chicken endocrine pancreas was investigated by using light microscopic immunohistochemistry and combined pre-embedding immunoperoxidase and post-embedding immunogold electron microscopic immunocytochemistry. Using the post-embedding method, immunoreactivity against the anti-insulin serum by protein A-gold technique was observed in the core of all types of B-cell granules. Immunoreaction with anti-S-100 protein serum was detected in the core of all types of B-cell granules. Immunoreaction with anti-S-100 protein serum was detected in the core of all types of B-cell granules from non-osmicated tissues even by post-embedding method, but immunoreactivities against the anti-AADC, DBH and CgA sera were only demonstrable in crystalloid granules of B-cells by pre-embedding method. Immunoreaction with the anti-CgA serum was also detected in the cytoplasmic matrix around crystalloid granules and also in the dense bodies showing immunonegative with anti-insulin serum. From these results, it seems likely that S-100 protein co-stored all types of B-cell granules involved in the maturation of granules, and AADC, DBH and CgA are related to the synthesis of noradrenaline in crystalloid granules of B-cells.
Department of Veterinary Anatomy, School of Agriculture, Nagoya University, Japan.
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M
Takayanagi
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T
Watanabe
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000032:analysis*; D002645:Chickens; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000737:chemistry* / Q000201:enzymology; D008297:Male; D008854:Microscopy, Electron; D009418:S100 Proteins / Q000032:analysis*
D053379:Chromogranin A; D002864:Chromogranins; D007328:Insulin; D009418:S100 Proteins; D004299:Dopamine beta-Hydroxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases