Replication and cytopathogenicity of human immunodeficiency virus type 1 (HIV-1)/simian immunodeficiency virus agm3 chimeric viruses in human and monkey cells: the 5' half of the HIV-1 genome is responsible for virus cytopathogenicity.
Two chimeric viruses were constructed between human immunodeficiency virus type 1 (HIV-1) and an apathogenic simian immunodeficiency virus (SIVagm3mc) from African green monkeys. One of the chimeras, HE-A391, expressed the HIV-1-derived env, vpu, tat and rev genes and the SIVagm3mc-derived LTR and the gag, pol and vif genes. The other chimera, SE-H13, contained the SIVagm3mc-derived env, tat and rev genes and the HIV-1-derived LTR and the gag, pol, vif and nef genes. Both constructs yielded infectious viruses and their phenotypes (growth-competence and cell-killing capacity) were examined in various CD4+ cells including human and monkey PBMCs. The results indicated that the replicative properties of the chimeras were mainly dependent on the 5'-genomic half of the parental viruses, and the determinant for viral cytopathogenicity was located within the 5' half of the HIV-1 genome.
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Japan.
M
M
Jin
E
E
Ido
T
T
Kuwata
T
T
Igarashi
K
K
Cichutek
R
R
Kurth
T
T
Miura
Y
Y
Enose
J
J
Chen
M
M
Hayami
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D016679:Genome, Viral; D054303:HIV Reverse Transcriptase / Q000032:analysis; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D000882:Haplorhini; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D014764:Viral Proteins; D054303:HIV Reverse Transcriptase