A radioimmunoassay for the determination of tipredane in human plasma.
A sensitive method for the determination of tipredane in human plasma has been developed. Prior to radioimmunoassay, proteins are removed from plasma by precipitation with acetonitrile. A [125I]iodohistamide derivative of tipredane is used as a heterogeneous radioligand. The primary antiserum is raised in sheep against a tipredane (O-carboxymethyl) oxime-bovine serum albumin conjugate. Donkey anti-sheep IgG antiserum is used as the secondary antiserum in a double antibody separation procedure. The limit of quantification of the method is 1 ng ml-1 for 500 microliters of plasma. There is no significant interference from either established or putative metabolites of tipredane, endogenous steroids or a wide range of other drugs. The assay was used to determine the concentrations of tipredane in plasma samples collected in pilot clinical studies.
Fisons plc, Department of Physical Chemistry, Loughborough, Leicestershire, UK.
M A
MA
Bayliss
J R
JR
Preston
D
D
Wilkinson
D J
DJ
Wilkinson
W J
WJ
Lockley
D000287:Administration, Topical; D000730:Androstadienes / Q000097:blood* / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000378:metabolism; D005938:Glucocorticoids; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D011863:Radioimmunoassay / Q000379:methods*
D000730:Androstadienes; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D007106:Immune Sera; C051756:tipredane