[Detection and characterization of anti-red cell autoantibodies in autoimmune hemolytic anemias].
Autoimmuine hemolytic anemias (AIHA) are classified into two groups of warm type and cold type according to the thermal properties of the anti-red cell autoantibody. A positive result of the antiglobulin test (DAT) confirms the presence of autoantibodies on red cells. DAT-negative AIHA are diagnosed by means of the elevation of red blood cell-associated IgG. The sera in low titer cold agglutinin disease show a low cold agglutinin titer but a high titer in the presence of bovine albumin. The antigenic specificity of warm reacting autoantibodies has been demonstrated, by using immunoblot and immunoprecipitation, such as Rh-related proteins, band 3, glycophorin A or Wrb antigen.
Department of Legal Medicine and Human Genetics, Jichi Medical School.
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Kamesaki
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Kajii
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D012710:Serum Albumin, Bovine / Q000276:immunology
D001323:Autoantibodies; D012710:Serum Albumin, Bovine