A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
The 718,122 base pair sequence of the Escherichia coli K-12 genome corresponding to the region from 12.7 to 28.0 minutes on the genetic map is described. This region contains at least 681 potential open reading frames, of which 277 (41%) have been previously identified, 147 (22%) are homologous to other known genes, 139 (20%) are identical or similar to the hypothetical genes registered in databases, and the remaining 118 (17%) do not show a significant similarity to any other gene. In this region, we assigned a cluster of cit genes encoding multienzyme citrate lyase, two clusters of fimbrial genes and a set of lysogenic phage genes encoding integrase, excisionase and repressor in the e14 genetic element. In addition, a new valine tRNA gene, designated valZ, and a family of long directly repeated sequences, LDR-A, -B and -C, were found.
Institute of Medical Science, University of Tokyo, Japan.
T
T
Oshima
H
H
Aiba
T
T
Baba
K
K
Fujita
K
K
Hayashi
A
A
Honjo
K
K
Ikemoto
T
T
Inada
T
T
Itoh
M
M
Kajihara
K
K
Kanai
K
K
Kashimoto
S
S
Kimura
M
M
Kitagawa
K
K
Makino
S
S
Masuda
T
T
Miki
K
K
Mizobuchi
H
H
Mori
K
K
Motomura
Y
Y
Nakamura
H
H
Nashimoto
Y
Y
Nishio
N
N
Saito
T
T
Horiuchi
D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D008040:Genetic Linkage; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D004269:DNA, Bacterial