Influence of dosage and timing of application of platelet-derived growth factor on early healing of the rat medial collateral ligament.
In previous studies, platelet-derived growth factor has demonstrated beneficial in vivo effects on wound healing. We report the results of two studies of platelet-derived growth factor in the rat medial collateral ligament injury model. Experimental injury sites were implanted with platelet-derived growth factor, whereas contralateral controls received only collagen. Twelve days postoperatively, the femur-medial collateral ligament-tibia complex was tested mechanically. Our first study found a marked drop in the effectiveness of platelet-derived growth factor when it was administered more than 24 hours after injury. Dose-response testing showed maximum increases in strength (90%) with 5.0 micrograms of platelet-derived growth factor, but the 1.0 microgram group showed similar strength increases, indicating a probable plateau effect in the response. These results indicate that platelet-derived growth factor has promise for healing ligaments but that it must be administered in appropriate doses soon after injury.
Batten
M L
ML
Department of Surgery, University of North Carolina at Chapel Hill, USA.
Hansen
J C
JC
Dahners
L E
LE
D000818:Animals; D001696:Biomechanical Phenomena; D004305:Dose-Response Relationship, Drug; D008297:Male; D017888:Medial Collateral Ligament, Knee / Q000293:injuries* / Q000503:physiopathology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012421:Rupture / Q000188:drug therapy / Q000503:physiopathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor