Drug substance manufacture process control: application of flow injection analysis and HPLC for monitoring an enantiospecific synthesis.
Effective process control can only be achieved through an understanding of the operating issues of the reaction. The development and use of effective and rugged analytical methods is necessary to monitor these parameters. The intent of this paper is to present some key analytical issues encountered in the synthesis of MK-0679, an LTD4 antagonist. In a key step of the compound's synthesis, a prochiral diester intermediate undergoes an enantioselective enzymatic hydrolysis (in the presence of Triton X-100) leading to the (S)-ester acid. Subsequent processing transforms the ester acid into the final product. The residual amount of the detergent in the final product, the rapid determination of the enzymatic activity and the optical purity of the final product emerged as key issues in the control of the reaction. As a solution, two techniques were utilized and are presented: flow injection analysis and HPLC.
Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
K
K
Chong
T
T
Loughlin
C
C
Moeder
H J
HJ
Perpall
R
R
Thompson
N
N
Grinberg
G B
GB
Smith
M
M
Bhupathy
G
G
Bicker
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D017998:Leukotriene D4 / Q000037:antagonists & inhibitors*; D017830:Octoxynol / Q000032:analysis; D011422:Propionates / Q000032:analysis / Q000138:chemical synthesis*; D011804:Quinolines / Q000032:analysis / Q000138:chemical synthesis*; D013237:Stereoisomerism; D013678:Technology, Pharmaceutical
D011422:Propionates; D011804:Quinolines; C059141:verlukast; D017998:Leukotriene D4; D017830:Octoxynol