Modification of the radiosensitivity of human cells to which simian virus 40 T-antigen was transfected.
Effects of the introduction of the Simian virus 40 T-antigen (SV40 T-Ag) gene to cultured human cells were examined in relation to radiosensitivity. Two relatively radioresistant tumor cell lines (T98 and G361) became significantly radiosensitive after the introduction of SV40 T-Ag, whereas radiosensitive tumor cell lines did not show a change in radiosensitivity. In contrast, a human fibroblast cell line became radioresistant after SV40 T-Ag introduction. T98 cells which have a mutation at codon 237 in the p53 gene were unable to form a complex between p53 protein and SV40 T-Ag, whereas G361, which became radiosensitive by a SV40 T-Ag introduction, formed the complex. This indicates that the status of p53 is independent of the change in radiosensitivity in the cell lines studied.
Yamagishi
N
N
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
Miyakoshi
J
J
Ohtsu
S
S
Day
R S
RS
Takebe
H
H
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D005347:Fibroblasts; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming