Differentiation-dependent enhanced expression of protein phosphatase 2Cbeta in germ cells of mouse seminiferous tubules.
The presence of five distinct isoforms of protein phosphatase 2Cbeta (PP2Cbeta-1 approximately -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cbeta-3, -4 and -5 and PP2Cbeta protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cbeta-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cbeta-4 determined with artificial substrates differed from those of PP2Cbeta-3 and -5, suggesting that the difference in the structure of PP2Cbeta-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
S
S
Kato
T
T
Kobayashi
K
K
Kusuda
Y
Y
Nishina
Y
Y
Nishimune
K
K
Yomogida
M
M
Yamamoto
H
H
Sakagami
H
H
Kondo
M
M
Ohnishi
N
N
Chida
Y
Y
Yanagawa
S
S
Tamura
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002454:Cell Differentiation; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012671:Seminiferous Tubules / Q000201:enzymology*; D013090:Spermatocytes / Q000166:cytology / Q000201:enzymology*; D013091:Spermatogenesis; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology
D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C087358:PTC1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C