Changes with age in the structure of fibromodulin in human articular cartilage.
An anti-peptide antibody was raised in a rabbit against the carboxy terminal region of the human fibromodulin core protein. The antibody was purified from other components of the resulting antiserum by affinity chromatography using the immobilized peptide, and was used to study the structural heterogeneity of fibromodulin extracted from human articular cartilage of different ages by the use of immunoblotting following sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the extracted macromolecules. In the fetus and neonate, fibromodulin was visualized as a diffuse component with a relative molecular weight of 70-110 kDa, whereas in the mature adult a more discrete component of smaller size was apparent with a relative molecular weight of 67 kDa. The size of the fibromodulin from mature adult cartilage could not be altered by pretreatment of the samples with keratanase II or endo-beta-galactosidase before analysis. In contrast, the size of the fibromodulin from younger cartilage could be decreased with both glycosidases, with the endo-beta-galactosidase yielding a smaller product than the keratanase. The size of the product resulting from endo-beta-galactosidase treatment of the fibromodulin from young cartilage was the same as that of the intact fibromodulin from mature adult cartilage. Thus, fibromodulin is present in human articular cartilage at all ages, but the extracted molecules only appear to exist in a proteoglycan form possessing keratan sulfate chains in the juvenile and young adult, and the size of these chains decreases with age. In the mature adult the fibromodulin does not possess either keratan sulfate or non-sulfated polylactosamine chains, though it appears to possess the same number of N-linked oligosaccharides as its counterparts from the younger tissue, but they are not modified further. The majority of the fibromodulin extracted from arthritic cartilage is of the same size as that found in the normal mature adult, although there is evidence for proteolytic processing. The degree of such processing is greater for the fibromodulin obtained from the cartilage of rheumatoid arthritic joints than osteoarthritic joints.
Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
P J
PJ
Roughley
R J
RJ
White
G
G
Cs-Szabó
J S
JS
Mort
D000118:Acetylglucosaminidase / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D002358:Cartilage, Articular / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D000071235:Fibromodulin; D006026:Glycoside Hydrolases; D006801:Humans; D015151:Immunoblotting; D007231:Infant, Newborn; D007632:Keratan Sulfate / Q000032:analysis; D008875:Middle Aged; D011509:Proteoglycans; D011817:Rabbits; D001616:beta-Galactosidase / Q000494:pharmacology
D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; C000604330:FMOD protein, human; D011509:Proteoglycans; D000071235:Fibromodulin; D007632:Keratan Sulfate; D006026:Glycoside Hydrolases; C086950:keratanase II; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase