Focal ischemia induces expression of the DNA damage-inducible gene GADD45 in the rat brain.
GADD45 is a DNA damage-inducible gene that accelerates DNA excision repair and can be induced by a variety of DNA-damaging stimuli in mammalian cells. We investigated the expression of GADD45 mRNA and protein using in situ hybridization and immunocytochemistry in rat brains after 2 h of temporal focal ischemia. The expression of GADD45 mRNA was induced in neurons throughout ischemic cortex 4 h after the onset of ischemia but was restricted to ischemic penumbra regions at 24 h after ischemia. The expression of GADD45 protein was increased only in sublethally injured neurons in the penumbra regions at both 4 h and 24 h following ischemia. These results suggest that GADD45 could have a protective role in ischemic neurons.
Department of Neurology, University of Pittsburgh, School of Medicine, PA 15261, USA.
K
K
Jin
J
J
Chen
K
K
Kawaguchi
R L
RL
Zhu
R A
RA
Stetler
R P
RP
Simon
S H
SH
Graham
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004247:DNA