Identification of the antigenic determinants of factors 8, 9, and 34 of genus Candida.
We investigated the antigenic determinants of factors 8, 9, and 34 of the genus Candida among pathogenic yeasts by enzyme-linked immunosorbent assay (ELISA) using mannans of Saccharomyces cerevisiae wild type and mutant types, mnn 1-mnn 4 and mnn 2. Results of ELISA including antisera against the antigenic factors of genus Candida (Candida Check, latron; FAbs) indicated that these three types of mannan distinctly react with FAbs 34, 8 and 9, respectively. To identify the recognition sites of these FAbs, we compared the ability of various oligosaccharides to inhibit the binding of the mannans to FAbs. The results indicated that FAb 34 preferentially recognizes linear side chains containing a non-reducing terminal alpha-1,3-linked mannose residue, Man(alpha)1 --> 3Man(alpha)1 --> (2Man(alpha)1 --> )n(2Man) (n > or = 0), and that one of the recognition sites of FAb 9 is linear alpha-1,6-linked oligomannosyl series, Man(alpha)1 --> (6Man(alpha)1 --> )n(6Man) (n > or = 2). On the other hand, the recognition site of FAb 8 apparently consisted of two alpha-1,2-linked oligomannosyl side chains and an alpha-1,6-linked mannose residue that originated from the mannan backbone, Man(alpha)1 --> 2Man(alpha)1 --> 2(Man(alpha)1 -->2Man(alpha)1 --> 6)Man.
Kobayashi
H
H
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Sendai, Miyagi, Japan.
Oyamada
H
H
Suzuki
A
A
Shibata
N
N
Suzuki
S
S
Okawa
Y
Y
D000946:Antigens, Fungal / Q000096:biosynthesis / Q000276:immunology*; D002175:Candida / Q000276:immunology* / Q000472:pathogenicity; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007106:Immune Sera; D008351:Mannans / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000946:Antigens, Fungal; D000939:Epitopes; D007106:Immune Sera; D008351:Mannans; D009844:Oligosaccharides; C028569:oligomannoside