Staurosporine inhibits the proliferation, alters the cell cycle distribution and induces apoptosis in HT-29 human colon adenocarcinoma cells.
Staurosporine (ST), a potent inhibitor of protein kinase C (PKC), was evaluated for its effect on the proliferation of HT-29 colon adenocarcinoma cells; PKC is associated with increased colon cell proliferation. ST inhibited cell proliferation in a time- and concentration-dependent manner by up to 90%. It also blocked the G2/M phase of the cell cycle and induced classical apoptosis (sub-diploid peak on flow cytometry, DNA ladder, and typical morphological changes). The kinetics of these changes suggest that low ST concentrations (2-20 nM) may act via a different mechanism from higher (100-1000 nM) ones. The role of ST, which is currently evaluated as an antitumor agent, in colon cancer requires further evaluation.
Qiao
L
L
Department of Medicine, Cornell University Medical College, New York, NY 10021, USA.
Koutsos
M
M
Tsai
L L
LL
Kozoni
V
V
Guzman
J
J
Shiff
S J
SJ
Rigas
B
B
D000230:Adenocarcinoma / Q000473:pathology*; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D019311:Staurosporine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D019311:Staurosporine