VIP-antiserum and indomethacin inhibit calcium and bicarbonate secretion by the inflamed feline gallbladder mucosa.
Ionized calcium (Ca2+) and bicarbonate (HCO(3)-) are important ions for gallstone formation. In the present study the effect of indomethacin and vasoactive intestinal peptide-antiserum (VIP-antiserum) on the Ca2+, HCO(3)- and fluid secretion in the inflamed gallbladder were tested in a validated experimental model in cats. The animals were studied in acute experiments 6 weeks after cystic duct ligation and gallstone implantation. During basal conditions there was a continuous secretion of Ca2+, HCO(3)- and fluid into the lumen of the inflamed gallbladder. Indomethacin and VIP-antiserum inhibited the Ca2+, HCO(3)- and fluid secretion across the inflamed gallbladder mucosa. Intraluminal amiloride inhibited the absorption of Ca2+, HCO(3)- and fluid after indomethacin infusion. An impaired absorption of Ca2+, HCO(3)- and fluid in the inflamed gallbladder is probably important for the solubility of calcium salts in the early stages of inflammation in the gallbladder mucosa.
Nilsson
B
B
Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
Delbro
D
D
Thune
A
A
Friman
S
S
Jivegård
L
L
Svanvik
J
J
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002415:Cats; D005260:Female; D005704:Gallbladder / Q000187:drug effects*; D007106:Immune Sera / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D001639:Bicarbonates; D007106:Immune Sera; D014660:Vasoactive Intestinal Peptide; D002118:Calcium; D007213:Indomethacin