Red blood cell aggregability is increased by aspirin and flow stress, whereas dipyridamole induces cell shape alterations: measurements by digital image analysis.
We evaluated the influence of pretreatment with aspirin in vitro, alone or combined with dipyridamole, on red cell aggregability. Samples were tested before and after being exposed to well-defined flow conditions. An aggregation rate was estimated through digital analysis of light microscopy images. Red cells of untreated blood that had been exposed to flow showed a higher aggregation rate (38.54 +/- 1.63 vs. 27.52 +/- 1.36; P < 0.05). This effect was not observed in the absence of platelets. Treatment with aspirin induced a high aggregation rate, with or without exposure to flow conditions. Dipyridamole alone or combined with aspirin provoked echinocytosis, disturbing the rouleaux arrangement (rates ranging from 11.25 +/- 1.91 - 13.62 +/- 1.62). Washing red cells after treatment restored about 90% of echinocytes to their biconcave shape, but aggregation rate did not recover in parallel. These results highlight the influence of red cell-platelet interactions in the regulation of haemostasis and show how therapeutic agents can interfere with rheological phenomena.
Servei d'Hemoteràpia i Hemostàsia, Hospital Clínic i Provincial de Barcelona, Villarroel, Spain.
J
J
Bozzo
M R
MR
Hernández
A
A
Del Giorgio
A
A
Ordinas
D001241:Aspirin / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004176:Dipyridamole / Q000494:pharmacology*; D004347:Drug Interactions; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D010975:Platelet Aggregation Inhibitors; D004176:Dipyridamole; D001241:Aspirin