Automated routine HLA-B27 typing by flow cytometry.
An increasing demand for HLA-B27 typing as one of the tests used in the diagnosis of ankylosing spondylitis had led us to develop a rapid, automated, flow cytometric assay using whole blood and an HLA-B27 specific monoclonal antibody FD705. This article shows the data from 2093 samples tested during a 2 year period of routine HLA-B27 typing. 21.6% were clearly HLA-B27 positive whilst 73.2% were HLA-B27 negative, the remaining 5.2% required further testing before assignment of HLA-B27 status. Additional work was carried out on blood samples from individuals positive for the newly described subtype HLA-B2708.
Wessex Histocompatibility Group, Southampton University NHS Hospital Trust, UK.
W M
WM
Reynolds
P R
PR
Evans
P J
PJ
Wilson
W M
WM
Wong
C
C
Darke
J L
JL
Smith
D000911:Antibodies, Monoclonal; D001331:Automation; D005434:Flow Cytometry / Q000379:methods; D015796:HLA-B27 Antigen / Q000032:analysis*; D006801:Humans; D012703:Serotyping / Q000379:methods; D013167:Spondylitis, Ankylosing / Q000175:diagnosis* / Q000276:immunology; D044465:White People
D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen