Cytokine-mediated inhibition of erythropoietin synthesis by dexamethasone.
The effect of inflammatory cytokines on the in-vitro synthesis of erythropoietin by HepG2 cells was evaluated. Monocyte-conditioned medium, and the cytokines interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha all reduced synthesis of erythropoietin. The steroidal anti-inflammatory drug, dexamethasone, did not affect cytokine-mediated erythropoietin inhibition. Dexamethasone did cause a reduction in the secretion of erythropoietin inhibitory cytokines from monocytes. These results point to a possible therapeutic approach in the treatment of anaemia caused by suppression of erythropoietin synthesis by monocytic cytokines.
Leng
H M
HM
Department of Pharmaceutical Chemistry, University of the Western Cape, Bellville, South Africa.
Kidson
S H
SH
Keraan
M M
MM
Randall
G W
GW
Folb
P I
PI
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004921:Erythropoietin / Q000096:biosynthesis*; D006801:Humans; D007202:Indicators and Reagents; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D017077:Culture Media, Conditioned; D016207:Cytokines; D007202:Indicators and Reagents; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D004921:Erythropoietin; D003907:Dexamethasone