Expression of feline immunodeficiency virus (FIV) gag gene in vaccinia virus vector.
The selected part of gag gene (nucleotide sequences at position 618 to 1724) coding for P24 and P15 proteins of feline immunodeficiency virus (FIV) was expressed in vaccinia virus vector under control of 7.5 kDa vaccinia virus transtriptional signal (p 7.5 vaccinia virus early/late promoter). The vaccinia virus recombinant (denoted VV-G(1)) was generated by homologous recombination following CV-1 cells transfection with a newly prepared vaccinia virus insertion vector -- pWKr75.
Department of Carnivore and Fur Animal Diseases, National Veterinary Research Institute, Pulawy, Poland.
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000235:genetics; D015750:Genes, gag / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D000882:Haplorhini; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D008969:Molecular Sequence Data; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics
D004274:DNA, Recombinant; D015683:Gene Products, gag; D014759:Viral Envelope Proteins; C079587:p15(gag) protein, Feline immunodeficiency virus; C074662:p24 protein, Feline immunodeficiency virus; D013937:Thymidine Kinase