Protective effect of taurine on hypoxia and reoxygenation-induced damage of human colon cells (HT 29).
In this experimental model, taurine administered during hypoxia markedly reduced the cell damage due to O2 deficiency, and the beneficial effect outlasted the period of reoxygenation. The mechanisms for the improved survival rates are postulated to be a reduced osmoregulatory disturbance of cellular integrity, improved Ca2+ homeostasis and induction of accelerated cellular growth processes. In our simplified cell culture model the UW solution seems to be the most appropriate solution for the cold (hypoxic) preservation of human colon cells. We conclude, that within this experimental model and under these experimental conditions, taurine supplementation of the conventionally used preservation solutions improved the solutions markedly. Considering our previous studies, taurine seems to be a potent endogenous protective agent against cellular deterioration due to hypoxia and reoxygenation.
Department of Pediatrics, University of Cologne, Federal Republic of Germany.
P
P
Wingenfeld
D V
DV
Michalk
A
A
Sonntag
S
S
Paas
T
T
Minor
W
W
Isselhard
D000241:Adenosine; D000332:Aerobiosis; D000493:Allopurinol; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003106:Colon; D003110:Colonic Neoplasms; D005947:Glucose; D005978:Glutathione; D006801:Humans; D006982:Hypertonic Solutions; D007328:Insulin; D008353:Mannitol; D019188:Organ Preservation Solutions; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D013654:Taurine / Q000494:pharmacology*; D014021:Tissue Preservation
C039984:Bretschneider cardioplegic solution; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D013654:Taurine; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose