Involvement of calcium in interactions between gingival epithelial cells and Porphyromonas gingivalis.
Porphyromonas gingivalis, a periodontal pathogen can invade primary cultures of gingival epithelial cells. This invasion was significantly inhibited (74-81%) by thapsigargin and 1,2-bis(2-aminophenoxy)ethane-N,N,N1,N1-tetraacetic acid, acetoxymethyl ester (BAPTA/AM), but not by EDTA or amiloride. Release of Ca2+ from an intracellular store and the subsequent increase in cytosolic [Ca2+] may, therefore, be involved in the invasion process, while Ca2+ influx is not. Moreover, cytosolic [Ca2+] was found to increase transiently in about 30% of gingival epithelial cells acutely exposed to P. gingivalis, but not in unexposed cells, or in cells exposed to noninvasive Escherichia coli. These findings indicate that P. gingivalis invasion of epithelial cells is correlated with activation of [Ca2+]-dependent host cell signaling systems.
Izutsu
K T
KT
Department of Oral Biology, University of Washington, Seattle 98195-7132, USA.
Belton
C M
CM
Chan
A
A
Fatherazi
S
S
Kanter
J P
JP
Park
Y
Y
Lamont
R J
RJ
D008841:Actin Cytoskeleton / Q000502:physiology; D000584:Amiloride / Q000494:pharmacology; D001422:Bacterial Adhesion; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003599:Cytoskeleton / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000502:physiology; D005881:Gingiva / Q000166:cytology / Q000382:microbiology*; D008870:Microtubules / Q000502:physiology; D016966:Porphyromonas gingivalis / Q000472:pathogenicity / Q000502:physiology*; D015290:Second Messenger Systems / Q000502:physiology*; D019284:Thapsigargin / Q000494:pharmacology
D002614:Chelating Agents; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D019284:Thapsigargin; D000584:Amiloride; D004492:Edetic Acid; D002118:Calcium