Topical gentamicin-induced hearing loss: a mitochondrial ribosomal RNA study of genetic susceptibility.
Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.
Department of Otolaryngology, Sunnybrook Health Science Centre, University of Toronto, Ontario, Canada.
J M
JM
Chen
P A
PA
Williamson
T
T
Hutchin
J M
JM
Nedzelski
G A
GA
Cortopassi
D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000009:adverse effects* / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D005260:Female; D005784:Gene Amplification; D005839:Gentamicins / Q000009:adverse effects* / Q000494:pharmacology* / Q000627:therapeutic use; D006311:Hearing Disorders / Q000139:chemically induced* / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008297:Male; D008575:Meniere Disease / Q000188:drug therapy / Q000503:physiopathology / Q000601:surgery; D008875:Middle Aged; D008928:Mitochondria / Q000187:drug effects*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000187:drug effects* / Q000235:genetics*; D014722:Vestibule, Labyrinth / Q000503:physiopathology / Q000601:surgery
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D012335:RNA, Ribosomal