Sialyl Lewis X analog inhibits eosinophil accumulation and late asthmatic response in a guinea-pig model of asthma.
Preferential eosinophil accumulation is characteristic of airway inflammation in asthma. Although little is known about its mechanism, the effect of a sialyl Lewis X analog on airway eosinophilia was examined in a guinea-pig model of asthma. Guinea-pigs were sensitized by repeated inhalation of ovalbumin. After a single inhalation challenge, the animals showed striking airway eosinophilia and a late asthmatic response. In contrast, when guinea-pigs were pretreated intravenously with sialyl Lewis X analog (LX 0104) 1 h before antigen challenge, both eosinophil infiltration in the tracheal wall and the late asthmatic response were significantly inhibited in a dose-dependent manner. In a further in vitro study, LX 0104 significantly suppressed the adhesion of human and guinea-pig eosinophils to human umbilical vein endothelial cells activated with interleukin-1 beta. These results suggest that LX 0104 plays a critical role in antigen-induced airway eosinophilia and the late asthmatic response.
Department of Internal Medicine and Clinical Immunology, Dokkyo University, School of Medicine, Tochigi, Japan.
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H
Sagara
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Ra
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T
Okada
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Shinohara
T
T
Fukuda
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K
Okumura
S
S
Makino
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001249:Asthma / Q000188:drug therapy*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003429:Cross Reactions; D004730:Endothelium, Vascular / Q000166:cytology; D004804:Eosinophils / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology*; D000080506:Sialyl Lewis X Antigen; D013997:Time Factors
D000893:Anti-Inflammatory Agents; C102020:LX 0104; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen