Altered prostanoid production by cumulus-oocyte complexes in a rat model of non-insulin-dependent diabetes mellitus.
Ovulation, oocyte maturation and PGE and PGF2 alpha production by oocyte-cumulus complexes were evaluated in rats with non-insulin-dependent diabetes induced by neonatal streptozotocin. Diabetic rats had normal estrous cycles, but ovulated a lower number of oocytes at estrus. When oocytes from control and diabetic rats obtained at proestrus were matured "in vitro" during 1, 2 or 4 hours (hr) of culture, differences were not found in the percent of germinal vesicle breakdown between both experimental groups. PGE and PGF2 alpha accumulation was higher in ovulated oocyte-cumulus complexes when compared to immature or "in vitro"-matured oocyte-cumulus complexes in both normal and diabetic rats. When control and diabetic rats are compared, more PGE and PGF2 alpha accumulation was observed in immature, "in vitro"-matured and in ovulated oocyte-cumulus complexes. A lower number of oocytes ovulated and increased oocyte-cumulus complexes prostaglandin production has been observed in this mildly diabetic experimental model. These abnormalities are similar to those previously found when 10 day embryos were evaluated in non-insulin-dependent diabetic rats.
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Serrano, Buenos Aires, Argentina.
A
A
Jawerbaum
E T
ET
Gonzalez
A
A
Faletti
V
V
Novaro
A
A
Vitullo
M A
MA
Gimeno
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D015237:Dinoprost / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004971:Estrus / Q000378:metabolism; D005260:Female; D008540:Meiosis; D009865:Oocytes / Q000502:physiology*; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D011458:Prostaglandins E; D015237:Dinoprost