Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI).
The cDNA encoding a Ypt/Rab guanosine dissociation inhibitor (Ypt-GDI) was isolated from the multicellular green alga Volvox carteri, representing the first complete plant gdi gene described. The gdiV1 gene occurs as a single copy in the algal genome, indicating that its product regulates all YptV proteins from Volvox. The derived GDI protein (GDIV1p) shows high similarity to animal and fungal GDIs. A specific antibody developed against GDIV1p detected the protein throughout the whole Volvox life-cycle. GDIV1p was localized in the cytoplasm and in the algal flagellum. This is in line with earlier findings of a dual localization of Ypt proteins both in the cell body and in the motility organelle, and indicates a novel role of the GDI/Ypt system, possibly in intraflagellar transport.
Universität Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, Germany.
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D020418:Algal Proteins; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000235:genetics; D000460:Chlorophyta / Q000737:chemistry* / Q000235:genetics; D003600:Cytosol / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D005407:Flagella / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D005796:Genes; D020730:Guanine Nucleotide Dissociation Inhibitors; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D020418:Algal Proteins; D018076:DNA, Complementary; D020730:Guanine Nucleotide Dissociation Inhibitors; D019204:GTP-Binding Proteins