Expression of the endogenous Marek's disease virus ICP4 homolog (MDV ICP4) gene is enhanced in latently infected cells by transient transfection with the recombinant MDV ICP4 gene.
The ICP4 homolog of Marek's disease virus (MDV ICP4) is a possible candidate for the transactivator of the early genes. We transfected MDCC-MSB-1 (MSB-1) tumor cells with plasmid including a coding region of MDV ICP4 using cationic liposome. As carriers for intranuclear transport, high mobility group -1 and -2 proteins were bound to the plasmid DNA before forming liposomes. We detected transcripts from the plasmid 2 hr after transfection by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis. We also detected abundant transcripts of endogenous ICP4 2-96 hr after transfection. These data suggested that expression of introduced MDV ICP4 gene enhanced the expression of endogenous MDV ICP4. On the other hand, quantitative PCR analysis for virus genome DNA indicated no significant alteration of copy number of virus genome in transfected MSB-1 cells, suggesting that reactivation of virus requires more than turning on MDV ICP4 gene.
Laboratory of Radiation Biology, Hokkaido University, Sapporo.
D
D
Endoh
S
S
Ikegawa
Y
Y
Kon
M
M
Hayashi
F
F
Sato
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002645:Chickens; D004274:DNA, Recombinant / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008223:Lymphoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D008380:Marek Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D004274:DNA, Recombinant; D004279:DNA, Viral; D012367:RNA, Viral