Enhancement of nucleocytoplasmic export of HTLV-1 Rex mRNA through cis and trans interactions of the mRNA with the complex of Rex protein and Rex-responsive element.
p27 rex of HTLV-1 promotes nucleocytoplasmic export of viral mRNAs through binding of the Rex-response element (RexRE) present at the 3' end of the viral transcripts in cis with respect to the ORFs of the viral mRNAs. We have found that expression of the RexRE in trans, as a separate RNA, still allows Rex protein to promote export of viral mRNAs lacking the RexRE. The data suggest the formation of a ternary complex between Rex protein, RexRE and upstream elements of viral mRNA and hence the existence of secondary sites of interaction between Rex protein and viral RNAs.
Division of Biochemistry and Molecular Biology, United Medical and Dental Schools of Guy's and St. Thomas's Hospitals, London, UK.
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D000818:Animals; D019556:COS Cells / Q000821:virology; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism / Q000821:virology; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000821:virology; D015967:Gene Expression Regulation, Viral; D016370:Gene Products, rex / Q000235:genetics / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D008956:Models, Chemical; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid
D016370:Gene Products, rex; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins