LITTLE KEVIN: a program for the estimation of protein homology by analysing the amino acid compositions and sequences.
We present here a computational method based on the analysis of amino acid composition for performing comparisons between proteins. This user-friendly and reliable test is aimed at rapidly identifying, from data-base subsets, sequences--if necessary, partial sequences--which share similar amino acid compositions to the input composition (deduced from experimental results). Apparent molecular weight (as determined by SDS-PAGE) and artefactual modifications due to the experimental determination of the amino acid composition are taken into account to perform the comparison. This program thus constitutes a useful tool in searching for the probable identification of either non-sequenced proteins or peptides from hydrolysed proteins.
INSERM U130, Marseille, France.
J P
JP
Grillasca
D
D
Aubert
R
R
Planells
N
N
Domingo
H
H
Lafont
D000465:Algorithms; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D016208:Databases, Factual; D005069:Evaluation Studies as Topic; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods* / Q000706:statistics & numerical data; D017386:Sequence Homology, Amino Acid; D012984:Software; D012985:Software Design
D000596:Amino Acids; D010446:Peptide Fragments; D011506:Proteins