Activity of choline acetyltransferase in the rat medial septal nucleus following fimbria-fornix transection or selective immunolesioning with 192 IgG-saporin.
Changes in the biochemical activity of choline acetyltransferase in the medial septal nucleus, diagonal band and hippocampus were determined following bilateral fimbria-fornix transection or selective immunolesioning of cholinergic septohippocampal neurons with 192 IgG-saporin. Following axotomy, choline acetyltransferase activity in the medial septal nucleus not only persisted but increased much above control values 6 months postlesion, confirming that many cholinergic neurons survive the transection of their axons. In contrast, immunolesioning led to a significant decrease in enzyme activity in the medial septal nucleus corresponding to the selective loss of septal cholinergic neurons in this lesion paradigm.
Institute of Anatomy, University of Freiburg, Germany.
B
B
Hage
M
M
Frotscher
T
T
Naumann
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D018678:Cholinergic Agents / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology / Q000601:surgery*; D000922:Immunotoxins / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D012686:Septal Nuclei / Q000166:cytology / Q000201:enzymology*
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D000922:Immunotoxins; D054789:Ribosome Inactivating Proteins, Type 1; D002795:Choline O-Acetyltransferase; D009699:N-Glycosyl Hydrolases; D000076985:Saporins