Serum rheumatoid factor induced by intraperitoneal administration of periodontopathic bacterial lipopolysaccharide in mice.
Serum rheumatoid factor (RF) level and peritoneal and splenic CD5+B (B-1) cells in mice were examined after intraperitoneal administration of purified lipopoly-saccharides (LPS) from oral periodontopathic bacteria; Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum and Capnocytophaga ochracea. F. nucleatum and C. ochracea LPS induced higher levels of serum IgM- and IgG-RF, while P. gingivalis LPS showed the least induction. In addition, wet weights of spleen and serum IgM and IgG concentration were markedly increased in F. nucleatum LPS injected group. On the other hand, the proportion of CD5+ B cells to lymphocytes in the peritoneal cavity and spleen did not increase. The reason for this was not clear but conventional B cells (CD5- B cells) might increase more rapidly with splenic enlargement than CD5+ B cells. These results suggested that RF induced by bacterial LPS may modulate immune responses against bacteria and plays an important role for defence and destruction of periodontal tissue.
Department of Periodontology, Nagasaki University School of Dentistry, Japan.
Y
Y
Hara
T
T
Kaneko
A
A
Yoshimura
I
I
Kato
D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology; D000704:Analysis of Variance; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018956:CD5 Antigens / Q000032:analysis; D002206:Capnocytophaga / Q000276:immunology; D005434:Flow Cytometry; D016967:Fusobacterium nucleatum / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000276:immunology; D012217:Rheumatoid Factor / Q000096:biosynthesis* / Q000097:blood; D013154:Spleen / Q000276:immunology; D013163:Splenomegaly / Q000276:immunology
D018956:CD5 Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D012217:Rheumatoid Factor