Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue.
The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.
Hanski
C
C
Department of Gastroenterology, Klinikum Benjamin Franklin der Freien Universität, Berlin, FRG.
Klussmann
E
E
Wang
J
J
Böhm
C
C
Ogorek
D
D
Hanski
M L
ML
Krüger-Krasagakes
S
S
Eberle
J
J
Schmitt-Gräff
A
A
Riecken
E O
EO
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D015152:Blotting, Northern; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D003110:Colonic Neoplasms / Q000201:enzymology / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005647:Fucosyltransferases / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002087:Butyrates; D016256:Lewis X Antigen; D012333:RNA, Messenger; D020148:Butyric Acid; D005647:Fucosyltransferases; D004121:Dimethyl Sulfoxide