cDNA cloning of human allograft inflammatory factor-1: tissue distribution, cytokine induction, and mRNA expression in injured rat carotid arteries.
We have previously identified a partial cDNA fragment obtained from rat carotid arteries subject to balloon angioplasty which is increased in response to vascular injury. We now report the isolation and characterization of a full length human clone which is 98% homologous at the amino acid level to the rat. Allograft Inflammatory Factor-1 (AIF-1), a gene previously reported to be expressed in rat IFN gamma-activated macrophages. This transcript is expressed in low levels in undamaged, and increases 1 day and 3 days, and declines 7 days post balloon angioplasty. This human AIF-1 homologue is inducible in serum and cytokine-stimulated human smooth muscle cells. Interestingly, human tissue distribution indicates a constitutive expression in lymphoid tissue which can be augmented by mitogens. In summary, human AIF represents a cytokine inducible, tissue-specific, and highly conserved transcript transiently expressed in response to vascular trauma.
Department of Molecular Biology, Deborah Research Institute, Browns Mills, New Jersey 08015, USA.
M V
MV
Autieri
D000595:Amino Acid Sequence; D000800:Angioplasty, Balloon; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D020212:Carotid Artery Injuries; D017536:Carotid Artery, Common / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D015870:Gene Expression; D006801:Humans; D008221:Lymphoid Tissue / Q000378:metabolism; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
C112986:AIF1 protein, human; C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; D008840:Microfilament Proteins; D012333:RNA, Messenger