Vaccination of C57BL/6 mice against cutaneous leishmaniosis. Kinetics of antibody synthesis and induction of cellular responses.
In this report, the effectiveness of vaccination of C57BL/6 mice and its association to long term production of IgG anti-Leishmania, delayed type hypersensitivity response (DTH) and the enlargement of the spleen associated to hyperplasia or parasite visceralization were studied. Mice were vaccinated with Leishmania-membrane antigens incorporated into liposomes, and two weeks later, animals received a homologous challenge with living amastigotes. Antibody levels were weekly recorded during 18 weeks after immunization. It was concluded that this vaccine induces disease-resistance in C57BL/6 mice and this effect correlates with the production of IgG anti-Leishmania, but not with the induction of a DTH or lymphoid hyperplasia.
Centro de Investigaciónes en Enfermedades Tropicales, Universidad Autónoma de Campeche, Mexico.
C M
CM
Lezama-Dávila
G
G
Gallagher
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology*; D007114:Immunization; D007700:Kinetics; D007894:Leishmania mexicana / Q000737:chemistry; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology
D000954:Antigens, Surface