Mapping of noninvasion TnphoA mutations on the Escherichia coli O18:K1:H7 chromosome.
The most virulent newborn meningitis-associated Escherichia coli are of the serotype O18:K1:H7. We previously isolated a large number of E. coli O18:K1:H7 mutants resulting from transposon TnphoA mutagenesis that fail to invade brain microvascular endothelial cells. We have now determined Ic locations of 45 independent insertions. Twelve were localized to the 98 min region, containing a 120 kb segment that is characteristic of E. coli O18:K1:H7. Another, the previously described insertion ibe-10::TnphoA, was localized to the 87 min region, containing a 20 kb segment found in this E. coli. These noninvasion mutations may define new O18:K1:H7 pathogenicity islands carrying genes for penetration of the blood-brain barrier of newborn mammals.
Department of Pediatrics and Epidemiology, University of Michigan, Ann Arbor 48109-0656, USA.
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Kim
D000469:Alkaline Phosphatase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001812:Blood-Brain Barrier; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004730:Endothelium, Vascular / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D016680:Genome, Bacterial; D006801:Humans; D007231:Infant, Newborn; D016920:Meningitis, Bacterial / Q000382:microbiology; D016254:Mutagenesis, Insertional; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012703:Serotyping; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase