Intracerebral tetracycline-dependent regulation of gene expression in grafts of neural precursors.
Tight control of the activity of a therapeutic gene introduced in vivo is a major issue in gene therapy research. Appropriate levels of expression may be crucial for gene correction. The tetracycline-sensitive regulatory system is highly effective for transcriptional regulation of foreign genes in mammalian cells. Here we report tight tetracycline-dependent regulation of a luciferase reporter gene transferred into the rat brain in the genetically modified neural precursor cell line ST14A as early as 2 days and until at least 6 days after transplantation. This is the first demonstration of the potential of this regulatory system for the modulation of the expression of therapeutic genes introduced into the central nervous system.
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, CNRS, Hôpital de la Pitié Salpêtrière, Paris, France.
O
O
Corti
P
P
Horellou
P
P
Colin
E
E
Cattaneo
J
J
Mallet
D000818:Animals; D001921:Brain / Q000187:drug effects*; D016380:Brain Tissue Transplantation; D002460:Cell Line; D017690:Cell Transplantation; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D018014:Gene Transfer Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013752:Tetracycline / Q000494:pharmacology*
D013752:Tetracycline