Migration of luteinizing hormone-releasing hormone (LHRH) neurons in early human embryos.
Luteinizing hormone-releasing hormone (LHRH) neurons originate in the epithelium of the medial olfactory pit and migrate from the nose into the forebrain along nerve fibers rich in neural cell adhesion molecule (N-CAM). The present study examined the ontogenesis of LHRH neurons in early human embryos and found a similar pattern of development of these cells. Luteinizing hormone-releasing hormone immunoreactivity was detected in the epithelium of the medial olfactory pit and in cells associated with the terminal-vomeronasal nerves at 42 (but not 28-32) days of gestation. The migration route of these cells was examined with antibodies to N-CAM and antibodies to polysialic acid (PSA-N-CAM), which is present on N-CAM at certain stages of development. Neural cell adhesion molecule immunoreactivity was present in a population of cells in the olfactory placode of the earliest embryos examined (28-32 days) and later (42 and 46 days) throughout the migration route. The PSA-N-CAM immunoreactivity was not detected until 42 days and was present in a more limited distribution in nerve fibers streaming from the olfactory placode and along the caudal part of the migration route below the forebrain. Previous studies have indicated that the highly sialated form of N-CAM is less adhesive. The PSA-N-CAM may therefore facilitate the migration of these cells by lessening the adhesion between the fascicles that make up the migration route, expediting the passage of cords of LHRH cells between the nerve fibers as these cells move toward the brain.
Rockefeller University, New York, USA.
M
M
Schwanzel-Fukuda
K L
KL
Crossin
D W
DW
Pfaff
P M
PM
Bouloux
J P
JP
Hardelin
C
C
Petit
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001923:Brain Chemistry; D004622:Embryo, Mammalian / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D019006:Neural Cell Adhesion Molecules / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D009474:Neurons / Q000378:metabolism*; D009666:Nose / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry
D000911:Antibodies, Monoclonal; D019006:Neural Cell Adhesion Molecules; D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; D007987:Gonadotropin-Releasing Hormone