The methylation pattern of a cytosine DNA-methyltransferase gene in Arabidopsis thaliana plants.
Using a PCR-amplified 5'-end proximal 600-bp fragment and two cDNA clones of cytosine DNA-methyltransferase gene of Arabidopsis thaliana as specific probes in hybridization with plant DNA samples, hydrolyzed by methylation-sensitive restriction endonucleases HpaII and MspI, it has been established that CCGG sites located in the 5'-end proximal part of cytosine DNA-methyltransferase gene are highly methylated at internal C and less but detectably methylated at external C residues. On the contrary, all CCGG sites in 3'-terminal half of the coding region were found to be unmethylated at both external and internal C residues. No significant differences between methylation patterns of cytosine DNA-methyltransferase gene in various organs (leaf, stem, flower) of the Arabidopsis thaliana plant were detected.
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow, Russia.
L I
LI
Kutueva
V V
VV
Ashapkin
B F
BF
Vanyushin
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D019175:DNA Methylation; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000235:genetics* / Q000378:metabolism; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D017343:Genes, Plant; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013379:Substrate Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; C029641:DNA modification methylase HpaII; D015257:DNA-Cytosine Methylases; D019003:Deoxyribonuclease HpaII