Should the asymmetry of enzymatic active centers always correlate with the asymmetry of their substrates?
Analysis of the substrate specificity of DNA polymerases and in part some other enzymes of nucleic acid metabolism towards unnatural L-stereomeric nucleosides and nucleotides was made. As a result, the hypothesis is proposed that when the chiral part of the substrate molecule does not interact specifically with the enzyme, substitution of natural D enantiomers by L compounds is possible.
Krayevsky
A A
AA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. AAK@imb.imb.ac.ru
Chernov
D N
DN
D001665:Binding Sites; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000737:chemistry* / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D004798:Enzymes; D004259:DNA-Directed DNA Polymerase