Age-related patterns of lectin-binding nuclear glycoproteins of hamster liver.
We studied the age-related patterns of lectin-binding liver nuclear glycoproteins from hamsters between 5 and 35 weeks of age. The examinations of the carbohydrate structures of liver nuclear glycoproteins in relation to the age of hamsters were carried out after electrophoresis and blotting by a very sensitive immunological detection system with highly specific digoxigenin-labelled lectins. The results reported in the present study do not show any significant changes in the patterns of nuclear glycoproteins with regards to the age of hamsters except the decrease in affinity of wheat germ agglutinin (WGA) to 63 kDa glycoprotein bearing single O-linked N-acetylglucosamine residues.
Krześlak
A
A
Department of Cytobiochemistry, University of Lódź, Poland.
Lipińska
A
A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D006224:Cricetinae; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D009687:Nuclear Proteins; D014909:Wheat Germ Agglutinins