Effects of dietary gluten on the hepatotoxic action of galactosamine and/or endotoxin in rats.
This study was done to clarify the effects of dietary wheat gluten on the hepatotoxic action of D-galactosamine (GalN) and endotoxin (Etx). Male Wistar rats fed a high casein or high gluten (supplemented with L-Lys and L-Thr) diet were injected with GalN or Etx, and the plasma glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactase dehydrogenase activities were examined 20 h later. In rats fed the high gluten diet, these enzyme activities were lower than in the high casein group after injection of 800 mg/kg of GalN. But such a difference between the casein and gluten groups was not clear when they were treated with 400 mg/kg of GalN nor observed even after injection of Etx or Etx+GalN (400 mg/kg). Similarly these was no difference in the plasma concentrations of Etx, tumor necrosis factor-alpha, or interferon-gamma in the rats receiving an injection of 800 mg/kg of GalN between both dietary groups. These results suggest that dietary gluten affords protection against hepatic injury by a high dose of GalN but not by a low dose of GalN and/or Etx.
Cheng
C C
CC
Department of Bioproduction Science, Chiba University, Japan.
Etoh
J
J
Tanimura
T
T
Egashira
Y
Y
Ohta
T
T
Sanada
H
H
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000502:physiology; D002364:Caseins / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000633:toxicity*; D005688:Galactosamine / Q000633:toxicity*; D005983:Glutens / Q000627:therapeutic use*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008107:Liver Diseases / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002364:Caseins; D004731:Endotoxins; D005688:Galactosamine; D005983:Glutens; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase