Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae.
Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
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Zachariae
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D000705:Anaphase; D018797:Cell Cycle Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D016213:Cyclins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016193:G1 Phase; D005800:Genes, Fungal; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism
C065697:BIME protein, Emericella nidulans; D018797:Cell Cycle Proteins; D016213:Cyclins; D005656:Fungal Proteins; D014452:Ubiquitins; D044767:Ubiquitin-Protein Ligases; D008025:Ligases