Indirect labeling of macroaggregated albumin with indium-111 via diethylenetriaminepentaacetic acid.
It is ideal to perform a simultaneous pulmonary perfusion and ventilation scan in cases of suspected pulmonary thromboembolism. Indium-111 (111In)-diethylenetriaminepentaacetic acid (DTPA)-macroaggregated albumin (MAA) was designed for this purpose. MAA was conjugated with DTPA at a molar ratio of 1:100 and incubated with 111In-chloride for 30 min at room temperature. DTPA-MAA could be labelled with 111In above a 96% labelling efficiency without MAA particle aggregates making their particles larger than desirable. The obtained 111In-DTPA-MAA was intravenously injected into normal mice and their biodistribution was studied at 15 and 180 min after injection. A gamma camera image was obtained 15 min after injection. 111In-DTPA-MAA was stable in vitro and in vivo, and gave high uptake of murine lung in the biodistribution study and clearly visualized murine lung in the scintigraph. Using 111In-DTPA-MAA as a pulmonary perfusion agent, a simultaneous pulmonary perfusion and ventilation scan with technetium-99m-ventilation agents is able to be performed using the dual-isotope technique. 111In-DTPA-MAA may be a potential pulmonary perfusion agent.
Department of Nuclear Medicine, Gunma University School of Medicine, Japan.
N
N
Watanabe
Y
Y
Shirakami
K
K
Tomiyoshi
N
N
Oriuchi
T
T
Hirano
M
M
Yukihiro
T
T
Inoue
K
K
Endo
D000418:Albumins / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D000818:Animals; D005260:Female; D015902:Gamma Cameras; D007205:Indium Radioisotopes; D008099:Liver / Q000000981:diagnostic imaging* / Q000378:metabolism; D008168:Lung / Q000000981:diagnostic imaging* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D013997:Time Factors; D014018:Tissue Distribution
D000418:Albumins; D007205:Indium Radioisotopes; C103803:indium DTPA-albumin; D004369:Pentetic Acid