[Immunoenzyme analysis for the identification of Mycobacterium bovis by using monoclonal antibodies].
Three monoclonal antibodies (MAb) were obtained after fusion of mouse BALB/c splenocytes immunised with gamma-irradiated field strains M. bovis and cells of mouse myeloma. Mab specificity was determined at enzyme immunoassay of the bacteria. The antibodies were able to identify various epitopes of the protein (molecular mass under 31 kD). One of the antibodies obtained served the basis for the test-system intended for rapid identification of M. bovis. The system requires 0.5-1 mg of the bacterial mass.
M V
MV
Androsova
M A
MA
Vladimirskii
O V
OV
Vasilenkova
L M
LM
Khodun
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D002417:Cattle; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000276:immunology* / Q000302:isolation & purification; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes