Adherence of human prostasomes to mouse spermatozoa and their displacement by monoclonal antibodies as revealed by free zone electrophoresis.
Prostasomes, small corpuscular organelles derived from the prostate gland, were isolated from human seminal plasma by means of ultracentrifugation and Sephadex G 200 chromatography to examine their interactive ability with mouse spermatozoa as assessed by free zone electrophoresis. During the run, human prostasomes and mouse spermatozoa approached each other and finally fused into one single peak that was not further dissociated. In some cases the fusion of prostasomes and spermatozoa failed to appear following pretreatment of prostasomes with monoclonal antibodies raised against these organelles.
Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden.
G
G
Ronquist
R
R
Fabiani
M
M
Jin
B O
BO
Nilsson
K
K
Elenbring
S
S
Hjerten
D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology; D004586:Electrophoresis / Q000379:methods*; D004822:Epididymis / Q000166:cytology*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015388:Organelles / Q000502:physiology*; D011467:Prostate / Q000648:ultrastructure*; D013094:Spermatozoa / Q000502:physiology*
D000911:Antibodies, Monoclonal