31P-magnetic resonance spectroscopy studies of nucleated and non-nucleated erythrocytes; time domain data analysis (VARPRO) incorporating prior knowledge can give information on the binding of ADP.
Human erythrocytes have no nucleus, mitochondria or endoplasmic reticulum, whereas chicken erythrocytes have a nucleus and mitochondria and are closer in internal morphology, to cells such as the hepatocyte. Erythrocytes were used to test the hypothesis that 31P-MRS invisibility of ADP is associated with the presence of intracellular organelles. Simple frequency domain spectral analysis methods showed that all the acid extractable ADP (and ATP) was MR-visible in human erythrocytes. However, such methods gave variable estimates for 31P-NMR spectra of fresh chicken erythrocytes from which no conclusions could be drawn about the MR-visibility of ADP. Only when the data were fitted by a method incorporating prior knowledge of the ATP and ADP peak structure, using the time domain VARPRO method, was it possible to conclude that in fresh chicken erythrocytes, similar to other nucleated cells (liver, muscle), all the acid extractable ADP appeared to be MRS invisible, indicating binding or sequestration by intracellular organelles.
Stubbs
M
M
CRC Biomedical Magnetic Resonance Research Group, St. George's Hospital Medical School, London, UK.
Van den Boogaart
A
A
Bashford
C L
CL
Miranda
P M
PM
Rodrigues
L M
LM
Howe
F A
FA
Griffiths
J R
JR
D000244:Adenosine Diphosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus; D002645:Chickens; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015388:Organelles; D010472:Perchlorates; D013997:Time Factors
D010472:Perchlorates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate