Crystallization and preliminary X-ray diffraction studies of N-acyl-D-glucosamine 2-epimerase from porcine kidney.
N-Acyl-D-glucosamine 2-epimerase from porcine kidney, which was cloned and expressed in Escherichia coli, was crystallized by the vapor-diffusion method, using polyethylene glycol and ammonium acetate as precipitants. The crystals were resistant to X-ray radiation damage and diffracted to more than 2.0 A resolution. The diffraction pattern indicated that the crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit-cell dimensions of a = 78.1, b = 97.2, and c = 100.7 A. It is supposed that the asymmetric unit consists of two N-acyl-D-glucosamine 2-epimerase molecules. Collection of data on the native crystals indicated that they are suitable for X-ray structural analysis.
Kyoto Research Laboratories, Marukin Shoyu Co., Ltd.
I
I
Maru
J
J
Ohnishi
Y
Y
Ohta
W
W
Hashimoto
Y
Y
Tsukada
K
K
Murata
B
B
Mikami
D000818:Animals; D002238:Carbohydrate Epimerases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D007668:Kidney / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D013552:Swine
D002352:Carrier Proteins; D011994:Recombinant Proteins; D002238:Carbohydrate Epimerases; C024519:N-acyl-D-glucosamine 2-epimerase