Bcl-2 transfection protects Hut78 cell line from different types of cytotoxic effector cells.
The human T lymphoma cell line Hut78 was transfected with Bel-2 gene or with control G418 Neomycin resistance-conferring plasmid. Bcl-2 transfected and Neomycin selected Hut78 cells expressed about ten-fold greater Bcl-2 protein than the neo-transfected controls. Susceptibility of Bcl-2-transfected Hut78 cells to human NK cells, human mixed lymphocyte reaction-generated cytotoxic effector cells, and mouse cytotoxic spleen cells generated by immunization with Hut78 cells was examined. In all systems tested, Bcl-2-transfected Hut78 target cells were significantly less susceptible to lysis than the neo-transfected control target cells. These results suggest that in Hut78 cells, Bcl-2 gene product may confer partial resistance to different types of cytotoxic effector mechanisms.
Saxena
R K
RK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Collins
G D
GD
Chrest
J F
JF
Adler
W H
WH
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000235:genetics*; D019254:Genes, bcl-2; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured