In vivo induction of interleukin-1 bioactivity in brain tissue after intracerebral infusion of native gp 120 and gp 160.
We have previously reported that intracerebral infusion of recombinant human immunodeficiency virus envelope protein gp 120 induced interleukin-1 (IL-1) bioactivity in rat brain. In this study, we tested the ability of native gp 120 and gp 160 to induce IL-1 activity in rat brain and also examined whether altering the secondary and tertiary structures of these proteins by carboxymethylation could influence the IL-1-inducing effect of these peptides. Results showed that both native gp 120 and gp 160 can induce IL-1 activity in rat brain in vivo, and that intact secondary and tertiary structures of these proteins appear critical for this effect.
Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, Ga, USA.
N
N
Quan
Z
Z
Zhang
M
M
Emery
E
E
Lai
R
R
Bonsall
V S
VS
Kalyanaraman
J M
JM
Weiss
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D019302:HIV Envelope Protein gp160 / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D007375:Interleukin-1