Alteration in central and peripheral substance P- and neuropeptide Y-like immunoreactivity after chronic hypoxia in the rat.
The influence of long-term hypoxia on substance P (SP) and neuropeptide Y (NPY)-like immunoreactivity (LI) in discrete brain areas and peripheral structures was assessed by radioimmunoassay. Rats were exposed to normobaric hypoxia (10% O2 in nitrogen) for 14 days. In the carotid bodies of hypoxic animals, NPY-LI was significantly increased (56% vs. normoxic controls) while SP-LI was unchanged. In the brain, NPY-LI was increased in the ventrolateral medulla oblongata (23%) and in the striatum (53%); however, SP-LI was unaltered in these two regions. In the anterior pituitary, NPY-LI was increased (99%), while SP-LI was decreased (37%). No significant alteration in NPY-LI and SP-LI was observed in other discrete brain areas or peripheral structures studied. These results show that, in the rat, long-term hypoxia induces changes in NPY-LI or SP-LI in a few central and peripheral structures; these biochemical alterations may be linked to adaptative mechanisms involving morphological changes in carotid bodies or alterations in sympathetic control and neuroendocrine function.
Département de Médecine Expérimentale, INSERM U 52, CNRS ERS 5645, Université Claude Bernard, Lyon, France. lponcet@cismsun.univ.lyonl.fr
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Poncet
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Denoroy
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Dalmaz
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Pequignot
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Jouvet
D000311:Adrenal Glands / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001923:Brain Chemistry; D002344:Carotid Body / Q000737:chemistry / Q000378:metabolism; D002908:Chronic Disease; D005728:Ganglia, Sympathetic / Q000737:chemistry / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D009478:Neuropeptide Y / Q000276:immunology* / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D009478:Neuropeptide Y; D011506:Proteins; D013373:Substance P