Sequence analysis and genetic mapping of porcine chromosome 11 centromeric S0048 marker.
We report the existence of a new family of swine centromeric satellite DNA composed of a 51-bp repeat unit, most specifically found on pig chromosome 11 centromere and with less specificity at the centromeric region of other meta- and submetacentric chromosomes. This satellite DNA family, which has no homologies with the Mc1 and Ac2 families published previously, was named Mc2. We designed a specific primer set for PCR amplification of this centromeric satellite DNA. Specificity of amplification was checked by using a porcine somatic cell hybrid panel and by FISH. Furthermore, the development of a PCR-RFLP marker of Mc2 repetition allowed its genetic mapping on the PiGMaP reference families panel. The centromere of chromosome 11 was thus integrated to the genetic map previously published.
Riquet
J
J
Laboratoire de Génétique Cellulaire, INRA BP27, Castanet-Tolosan France. riquet@toulouse.inra.fr
Mulsant
P
P
Yerle
M
M
Cristobal-Gaudy
M S
MS
Le Tissier
P
P
Milan
D
D
Gellin
J
J
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003360:Cosmids; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D006822:Hybrid Cells / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012377:Rodentia; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers