Quantitative PCR with colorimetric detection.
We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of PCRs made with various amounts of standard. This also enables the control of the consistency of the data and elimination of some artifactual results.
Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris.
F
F
Demay
S
S
Darche
P
P
Kourilsky
S
S
Delassus
D000199:Actins / Q000032:analysis / Q000235:genetics; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000199:Actins; D016753:Interleukin-10