Determination of gabapentin in serum using solid-phase extraction and gas-liquid chromatography.
A gas-liquid chromatographic method for the determination of gabapentin (Neurontin) is described. The method involves extracting 0.5 mL of acidified sample by C18 solid-phase column, derivatization with MTBSTFA plus 1% tBDMCS, and analysis on an HP-1 column with a flame-ionization detector. Quantitation was performed with peak-height ratios of gabapentin to a gabapentin analogue [(1-aminomethyl-1-cycloheptyl) acetic acid] as the internal standard. The assay had a limit of detection of 0.2 mg/L and a linear range from 0.5 to 30.0 mg/L. Several compounds were analyzed for potential interference, and none interfered with the assay.
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0165, USA.
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CE
Wolf
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JJ
Saady
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A
Poklis
D000081:Acetamides; D000085:Acetates / Q000097:blood*; D000588:Amines; D000927:Anticonvulsants / Q000097:blood*; D002138:Calibration; D002849:Chromatography, Gas; D003508:Cycloheptanes / Q000097:blood; D003509:Cyclohexanecarboxylic Acids; D005410:Flame Ionization; D005463:Fluoroacetates; D000077206:Gabapentin; D006801:Humans; D017646:Organosilicon Compounds / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry; D014269:Trifluoroacetic Acid / Q000737:chemistry; D005680:gamma-Aminobutyric Acid
D000081:Acetamides; D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003508:Cycloheptanes; D003509:Cyclohexanecarboxylic Acids; D005463:Fluoroacetates; D017646:Organosilicon Compounds; D012821:Silanes; C103257:gabapentin ISTD; C103256:tert-butyldimethylsilane; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D014269:Trifluoroacetic Acid