Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.
cDNA encoding a novel human ubiquitin-conjugating enzyme has been cloned from an epidermoid carcinoma KB cDNA library. This clone encodes a protein of 152 amino acids with a calculated M(r) of 17,137. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. The product expressed in Escherichia coli exhibited the ability to form a thiol ester linkage with ubiquitin in a ubiquitin-activating enzyme E1-dependent manner. These results suggest that the obtained cDNA encodes a novel human E2 which may be involved in protein degradation mainly in the muscles and testis.
Sagami Chemical Research Center, Kanagawa.
T
T
Yamaguchi
N S
NS
Kim
S
S
Sekine
H
H
Seino
F
F
Osaka
F
F
Yamao
S
S
Kato
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics; D029721:Drosophila Proteins; D004926:Escherichia coli; D005260:Female; D015870:Gene Expression; D006801:Humans; D007624:KB Cells; D008025:Ligases / Q000096:biosynthesis* / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D015183:Restriction Mapping; D013737:Testis / Q000201:enzymology; D014158:Transcription, Genetic; D044763:Ubiquitin-Conjugating Enzymes
D018076:DNA, Complementary; D029721:Drosophila Proteins; D044763:Ubiquitin-Conjugating Enzymes; C471544:ben protein, Drosophila; D008025:Ligases