Anti-glomerular basement membrane disease: role of enzyme-linked immunosorbent assays in diagnosis.
We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivity, 97.0% specificity). The second ELISA kit was much less sensitive (87.8% agreement, 63.3% sensitivity, 92.2% specificity). ELISA tests for the measurement of anti-GBM antibodies allow for quantitation and may have greater sensitivity than IFA. Sensitivity, however, may depend on the commercial test used.
Department of Pathology, University of Utah, Salt Lake City 84132, USA.
C M
CM
Litwin
C L
CL
Mouritsen
P A
PA
Wilfahrt
M C
MC
Schroder
H R
HR
Hill
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019867:Anti-Glomerular Basement Membrane Disease / Q000175:diagnosis*; D000906:Antibodies / Q000378:metabolism; D001485:Basement Membrane / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008875:Middle Aged
D000906:Antibodies