The N-formyl methionyl peptide, formyl-methionyl-leucyl phenylalanine (fMLF) increases the lateral diffusion of complement receptor 1 (CR1/CD35) in human neutrophils; a causative role for oxidative metabolites?
The effects of the N-formyl methionyl peptide, formyl-methionyl-leucyl phenylalanine (fMLF) on the lateral mobility of the complement receptor type 1 (CR1/CD35) in glass-adherent human neutrophils were investigated, using fluorescence recovery after photobleaching (FRAP) and confocal microscopy (CSLM). It was found that addition of 0.1-1 microM fMLF increased the diffusion constant (D) of CR1/CD35 to 167-228% of controls. No effect was observed on the receptor distribution or the mobile fraction of receptors. The effect of fMLF on the lateral diffusion of CR1/CD35 could be totally inhibited by addition of pertussis toxon (PD, 250 ng/ml) or of the free radical scavenger enzymes superoxide dismutase (SOD, 2000 U/ml) and catalase (CAT, 200 U/ml), added together the results show that oxidative metabolites produced by neutrophils in response to fMLF can modulate CR1/CD35 diffusion, and indicate a regulatory role for oxygen radicals in phagocytosis.
Dept. of Medical Microbiology, Faculty of Health Sciences, University of Linköping, Sweden.
B J
BJ
Rasmusson
J L
JL
Carpentier
J P
JP
Paccaud
K E
KE
Magnusson
D002374:Catalase / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013696:Temperature; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D017382:Reactive Oxygen Species; D011951:Receptors, Complement; D010566:Virulence Factors, Bordetella; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002374:Catalase; D013482:Superoxide Dismutase