The activity of the gluconate-H+ symporter of Schizosaccharomyces pombe cells is down-regulated by D-glucose and exogenous cAMP.
Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable analogues to glycerol-grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down-regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by D-glucose but also by extracellular cAMP even in the absence of the cAMP-dependent protein kinase (PKA1).
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany. thomas.caspari@biologie.uni-regensburg.de
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D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D012568:Schizosaccharomyces / Q000187:drug effects / Q000378:metabolism*
D002352:Carrier Proteins; D005942:Gluconates; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D005947:Glucose; C030691:gluconic acid