Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay.
By combining the enterohemolysin test and the VTEC-RPLA test (specific for the detection of Shiga-like toxin I [SLT-I], SLT-II, and SLT-IIc), single colonies of SLT-producing Escherichia coli were found to constitute between 0.03 and 68.1% of the coliform flora in human stool cultures and were isolated and characterized within 72 to 96 h.
Department of Microbiology, Robert Koch-Institut, Berlin, Germany.
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Beutin
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Zimmermann
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Gleier
D000362:Agar; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D001769:Blood; D003092:Colitis / Q000175:diagnosis / Q000382:microbiology; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006461:Hemolysis; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D012756:Sheep; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D022622:Shiga Toxin 1; D000362:Agar