Subcellular redistribution of HSP72 protein during cisplatin-induced apoptosis in HeLa cells.
Exposure of HeLa cells to cisplatin results in the activation of apoptotic cell death. This drug induces DNA damage and generates reactive oxygen intermediates. Since cisplatin is highly reactive and binds to diverse proteins, it could create abnormal protein structures or nonspecific aggregates. For these reasons, we analyzed the expression and subcelullar distribution of hsp72, a heat-shock protein that enables cells under stress to cope with damaged proteins. We did not observe any changes in the expression of hsp72 protein, although, by immunofluorescence studies, we detected a dramatic redistribution of the protein. These results and its probable relevance in the drug-induced apoptotic phenomenon are discussed.
División de Investigación Básica, Instituto Nacional de Cancerologia, Tlalpan, Mexico, D.F. zajgla@cenids.ssa.gob.mx
J
J
Meléndez-Zajgla
C
C
García
V
V
Maldonado
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D004249:DNA Damage; D050884:HSP72 Heat-Shock Proteins; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017382:Reactive Oxygen Species; D013997:Time Factors
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D002945:Cisplatin