Cell death induced by a 131I-labeled monoclonal antibody in ovarian cancer multicell spheroids.
Treatment of OVCAR-3 spheroids with 131I-OC125 monoclonal antibody produced a decrease in spheroid volume and a concomitant rise in necrotic cell number. No increase in apoptotic cell number was observed during incubation of spheroids with the labeled antibody. Necrosis began early, reaching a maximum after 3 Gy of accumulated dose delivered at a dose rate of 1.8 cGy/h. Higher accumulated doses induced necrosis for longer incubation times. Thus, dose rate and time are both determinants of ultimate radiation effects when spheroids are incubated with labeled antibodies, although dose rate is the most important factor.
Inserm Unité 211, Institut de Biologie, Quai Moncousu, Nantes, France.
I V
IV
Filippovich
N
N
Sorokina
N
N
Robillard
A
A
Faivre-Chauvet
M
M
Bardies
J F
JF
Chatal
D000911:Antibodies, Monoclonal; D016923:Cell Death / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D010051:Ovarian Neoplasms; D016499:Radioimmunotherapy; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes