Biological formation of the 1,3-butadiene DNA adducts 7-N-(2-hydroxy-3-buten-1-yl)guanine, 7-N-(1-hydroxy-3-buten-2-yl)guanine and 7-N-(2,3,4-trihydroxy-butyl)guanine.
Male mice (B6C3F1) and male Wistar rats were exposed to [1,4-(14)C] butadiene in a closed all-glass desiccator jar system, and liver DNA was obtained. After acid hydrolysis and HPLC chromatography radioactive 7-N-(1-hydroxy-3-buten-2-yl)guanine and 7-N-(2,3,4-trihydroxybutyl)guanine were identified as radioactive DNA adducts. The same DNA adducts were identified upon incubation of calf thymus DNA, rat liver microsomes, [1,4-(14)C]butadiene and an NADPH-regenerating system. In this system, a third radioactive adduct was found which was identified as 7-N-(2-hydroxy-3-buten-yl)guanine.
Institut für Arbeitsphysiologie an der Universität Dortmund, Germany.
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D000818:Animals; D002070:Butadienes / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism*; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
D002070:Butadienes; D002273:Carcinogens; D018736:DNA Adducts; D006147:Guanine; C031763:1,3-butadiene