[Analysis of genetic properties of meiotic products from an interspecific triploid fusion-hybrid itself constructed by protoplast fusion in Saccharomvces].
A genetically stable interspecific triploid fusion-hybrid between a diploid Saccharomyces cerevisiae var. ellopsoideus and a haploid Saccharomyces diastaticus was constracted by protoplast fusion technique. Experimental results indicate that such hybrid can be induced to sporulate as a sexual hybrid. Genetic analysis of the complete and non-complete tetrads demonstrates that the prototrophic interspecific triploid fusion-hybrid HU-KDF-240 selected by nutritional complementation experienced segregation and exchange for the genetic markers, and showed parental ditype and recombination in the meiotic process of sporulation. The results of the tetrad analysis of the hybrid HU-KDF-240 showed a 1:2 segregation ratio for soluble starch fermenting and non-fermenting characters and a 1:1 segregation ratio for genetic markers of the auxotrophic character in 349 complete and non-complete tetrads from 186 asci of the hybrid.
Department of Biological Science and Biotechnology Hangzhou University.
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Li
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Zhao
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Du
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Zhi
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Chen
D003001:Cloning, Molecular; D008540:Meiosis; D011123:Polyploidy; D011523:Protoplasts / Q000166:cytology*; D012440:Saccharomyces / Q000166:cytology / Q000235:genetics*