Comparison of the activity of carp and rat beta-actin gene regulatory sequences in tilapia and rainbow trout embryos.
A comparative study on the level of expression of lacZ reporter constructs driven by equivalent carp and rat beta-actin regulatory sequences was carried out in embryos of tilapia and rainbow trout. DNA was microinjected into fertilised tilapia and rainbow trout eggs and the embryos/fry were assayed at various developmental stages for beta-galactosidase expression. We provide evidence to demonstrate that the carp beta-actin promoter/ lacZ reporter gene is expressed at higher levels than the equivalent rat beta-actin construct in both species.
Alam
M S
MS
Department of Biology, School of Biological Sciences, University of Southampton, United Kingdom.
Lavender
F L
FL
Iyengar
A
A
Rahman
M A
MA
Ayad
H H
HH
Lathe
R
R
Morley
S D
SD
Maclean
N
N
D000199:Actins / Q000235:genetics*; D000818:Animals; D002347:Carps; D004625:Embryo, Nonmammalian / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005809:Genes, Regulator; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000235:genetics*; D011247:Pregnancy; D051381:Rats; D017210:Tilapia / Q000196:embryology / Q000235:genetics*
D000199:Actins