Folding and aggregation of DNA chains induced by complexation with lipospermine: formation of a nucleosome-like structure and network assembly.
Dioctadecylamidoglycylspermine (DOGS) is a cationic lipid vector capable of efficiently introducing DNA into various eukaryotic cells. We investigated the higher-order structure of the DNA/DOGS complex using fluorescence and electron microscopy. Our results show that the DNA/DOGS complex exhibits a nucleosome-like structure in which DNA wraps around an aggregate of DOGS molecules. In addition, DNA/DOGS complexes tend to associate with each other to form network structures. The resulting network assembly may play a role in effective gene transfection.
Yoshikawa
Y
Y
Graduate School of Human Informatics, Nagoya University, Japan.
Emi
N
N
Kanbe
T
T
Yoshikawa
K
K
Saito
H
H
D000818:Animals; D017122:Bacteriophage T4 / Q000235:genetics; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D005347:Fibroblasts; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D008854:Microscopy, Electron; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry*; D051381:Rats; D012996:Solutions; D013096:Spermine / Q000031:analogs & derivatives* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D009707:Nucleosomes; D012996:Solutions; C061134:dioctadecylamidoglycylspermine; D013096:Spermine; D004247:DNA; D005998:Glycine