Effect of the ACAT inhibitor, HL-004, on cholesterol metabolism in macrophages.
The effects of a novel acyl-CoA: cholesterol acyltransferase (ACAT) inhibitor, HL-004, on cholesterol metabolism were examined in mice peritoneal macrophages. Cholesteryl ester-rich foam cells were induced by incubating macrophages with acetylated LDL. HL-004 prevented the accumulation of cholesteryl ester in the presence of the cholesterol acceptor, HDL. In the absence of HDL, HL-004 generated large amounts of free cholesterol in the cell. Moreover, HL-004 stimulated the efflux of cholesterol from preestablished foam cells in the presence of HDL. These results suggest that the inhibition of foam cell formation and the stimulation of foam cell regression by HL-004 are attributed to intracellular ACAT inhibition, and that HL-004 would be expected to exhibit an antiatherosclerotic effect through direct action on arterial wall.
Murakami
S
S
Medicinal Research Laboratories, Taisho Pharmaceutical Co., Saitama, Japan.
Yamagishi
I
I
Asami
Y
Y
Sato
M
M
Tomisawa
K
K
D000083:Acetanilides / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D000083:Acetanilides; D004791:Enzyme Inhibitors; C092284:HL 004; D002784:Cholesterol; D002785:Sterol O-Acyltransferase