Titration of a vaccine stock preparation of human immunodeficiency virus type 1SF2 in cultured lymphocytes and in chimpanzees.
A large stock preparation of the HIV-1SF2 isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 10(4.9) TCID50 in human PBMCs and 10(4.2) TCID50 in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infectious dose titer was found to be about 10(2.3). Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190 degrees C, are available for future studies of vaccines and antiviral therapies.
Murthy
K K
KK
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78245, USA.
Cobb
E K
EK
el-Amad
Z
Z
Ortega
H
H
Hsueh
F C
FC
Satterfield
W
W
Lee
D R
DR
Kalish
M L
ML
Haigwood
N L
NL
Kennedy
R C
RC
Steimer
K S
KS
Schultz
A
A
Levy
J A
JA
D016915:AIDS Vaccines; D000818:Animals; D004279:DNA, Viral / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D015497:HIV-1 / Q000472:pathogenicity; D006801:Humans; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry
D016915:AIDS Vaccines; D004279:DNA, Viral; D012367:RNA, Viral