Vitamin D3 elicits calcium response and activates blood monocyte-derived macrophages from patients with vitamin D dependent rickets type II.
We studied the effects of vitamin D3 metabolites on intracellular free Ca2+ concentration ([Ca2+]i) and the respiratory burst of monocyte-derived macrophages (MDM) from patients with vitamin D dependent rickets type II. Treatment of MDM from the patients and healthy donors with 1 nM 1,25(OH)2D3 produced a rapid elevation of [Ca2+]i and similarly primed both types of cells for enhanced capacity for O2- release with phorbol diester. These results suggest that macrophages may have distinct non-genomic pathways of vitamin D3, which partly explain the absence of immunodeficiency and the disappearance of rickets after treatment with vitamin D3 in the patients.
Takeda
E
E
Department of Clinical Nutrition, School of Medicine, The University of Tokushima, Tokushima City, Japan.
Takata
K
K
Yamanaka
H
H
Taketani
Y
Y
Morita
K
K
Miyamoto
K
K
Shono
M
M
Teshima
S
S
Rokutan
K
K
D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002112:Calcifediol / Q000494:pharmacology; D002117:Calcitriol / Q000031:analogs & derivatives / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D012279:Rickets / Q000097:blood / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D015650:24,25-Dihydroxyvitamin D 3; D000255:Adenosine Triphosphate; D002117:Calcitriol; C051883:maxacalcitol; D002112:Calcifediol; D002118:Calcium