Incorporating anomalous scattering centres into macromolecules.
The widespread application of multiwavelength anomalous diffraction (MAD) for phase evaluation has been hampered in the past by the small selection of anomalous scattering centres that could be introduced into macromolecules. Recently, the use of chemical modification, protein engineering or biosynthetic labelling has provided suitable tools to overcome the previous limitations, thereby making most structural analyses amenable to a MAD approach.
Structural Biology Laboratory, Imperial Cancer Research Fund, London, UK.
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Pappa
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Stewart
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McDonald
D000818:Animals; D018360:Crystallography, X-Ray; D006801:Humans; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry*
D011506:Proteins