B cell hybridoma presents both B-cell and T-cell epitopes for stimulating antibody production via CD23 pathway.
CD23+ B cell hybridoma 17A11, pulsed with IgE:TNP-KLH triggered IgA, IgG, and IgE antibody production via CD23-mediated presentation. Prior anti-CD23 treatment abrogated 95% of the humoral antibody responses. Both B and T cell epitopes were presented by 17A11 B cell epitopes as recognized by IgG but not T cell epitopes were sensitive to treatment with 0.2 M acetic acid. Efficacy of antigen presentation via CD23 on 17A11 was comparable to that mediated via surface immunoglobulins (sIg) on a CD23 negative 4.5 parental fusion partner B cell line. This is the first demonstration that IgE:TNP-KLH pulsed B cell hybridomas present both B- and T-cell epitopes in stimulating IgA, IgG, and IgE antibody production, and raise a pertinent issue whether IgE antibodies produced under pathophysiological conditions may serve as positive feedback signal for sustaining production of different classes of antibodies.
Department of Veterinary Science, IANR, University of Nebraska-Lincoln, USA.
R
R
Schmaltz
Y Y
YY
Wang
Q X
QX
Kung
F T
FT
Liu
T
T
Petro
S S
SS
Chen
D000818:Animals; D000917:Antibody Formation; D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D017455:Receptors, IgE / Q000502:physiology*
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D011947:Receptors, Antigen, B-Cell; D017455:Receptors, IgE