Genomic PCR detects tumor cells in peripheral blood from patients with myxoid liposarcoma.
Myxoid liposarcoma (MLS) is the most common subtype of liposarcoma. The cytogenetic hallmark of MLS is the pathognomonic t(12;16)(q13;p11), present in more than 85% of cases. The translocation leads to the fusion of the CHOP and FUS genes at 12q13 and 16p11, respectively, and the generation of a FUS/CHOP hybrid protein. The presence of a tumor-specific chimeric gene makes it possible to identify MLS cells by polymerase chain reaction (PCR). We have analyzed peripheral blood samples obtained during a 10-year period at diagnosis of primary and/or recurrent disease in 19 MLS patients with t(12;16) and in one MLS patient with t(12;22;20), resulting in the fusion of the CHOP and EWS genes. Nested PCR on genomic DNA from blood samples amplified FUS/CHOP hybrid fragments in three patients and EWS/CHOP in the patient with t(12;22;20). There was no obvious association between PCR findings and clinical outcome, but larger series are needed to draw any firm conclusions.
Panagopoulos
I
I
Department of Clinical Genetics, University Hospital, Lund, Sweden.
Aman
P
P
Mertens
F
F
Mandahl
N
N
Rydholm
A
A
Bauer
H F
HF
Mitelman
F
F
D000328:Adult; D022762:CCAAT-Enhancer-Binding Proteins; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000097:blood; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D018208:Liposarcoma, Myxoid / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D034802:RNA-Binding Protein EWS; D034702:RNA-Binding Protein FUS; D012261:Ribonucleoproteins / Q000235:genetics; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics
D022762:CCAAT-Enhancer-Binding Proteins; C497249:DDIT3 protein, human; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009363:Neoplasm Proteins; D034802:RNA-Binding Protein EWS; D034702:RNA-Binding Protein FUS; D012261:Ribonucleoproteins; D014157:Transcription Factors; D051742:Transcription Factor CHOP