CD3 gamma, CD3 delta, and CD3 zeta mRNA in adult human marrow hematopoietic progenitors correlates with surface CD2 and CD7 expression.
Hematopoietic stem cells from adult marrow, cord blood, or fetal liver can differentiate into myeloid and lymphoid lineages. Early steps in this differentiation process are not yet fully understood. To correlate surface antigen expression with molecular events occurring during early lymphopoietic differentiation, we examined CD3 gamma, CD3 delta, and CD3 zeta gene expression in adult human CD34+ marrow progenitors and their subsets. Purification by fluorescence-activated cell sorter (FACS) was used to obtain 1) a CD34+ Lin-DR- population known to contain primitive, uncommitted progenitors; 2) CD34+/CD7+/CD2+ and 3) CD34+/CD7+/CD2+ cells expressing receptors associated with natural killer (NK) cell or T cell lineage commitment. We demonstrate that CD34+Lin-DR- cells do not contain CD3 gamma, CD3 delta, or CD3 zeta transcripts, consistent with the primitive uncommitted nature of progenitors in this cell population. Expression of the CD34+/CD7+/CD2- phenotype correlates with the transcription of CD3 zeta but not CD3 gamma or CD3 delta, a pattern of transcription observed in mature blood NK but not T cells. Expression of both CD7 and CD2 on CD34+ cells is associated with not only CD3 zeta gene transcription but also CD3 gamma and CD3 delta, a pattern found in T cells but not mature NK cells. We have identified unique patterns of mRNA transcription in phenotypically distinct lymphoid progenitors found in the marrow. These findings raise the possibility that although primitive NK and T cell progenitors share a common differentiation pathway, divergent NK and T lineage commitment steps may occur very early in lymphopoiesis. Our findings suggest that application of in vitro marrow and thymus culture techniques may be utilized to more fully describe commitment and differentiation of early lymphoid progenitors and define the role of the microenvironment in this process.
Division of Hematology, University of Minnesota Medical School, Minneapolis 55455, USA.
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H
Long
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P
Gaffney
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F
Mortari
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JS
Miller
D000328:Adult; D019011:Antigens, CD7 / Q000235:genetics*; D001854:Bone Marrow Cells; D018801:CD2 Antigens / Q000235:genetics*; D017252:CD3 Complex / Q000235:genetics*; D002454:Cell Differentiation; D002460:Cell Line / Q000166:cytology; D002469:Cell Separation; D017931:DNA Primers; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology; D014158:Transcription, Genetic
D019011:Antigens, CD7; D018801:CD2 Antigens; D017252:CD3 Complex; D017931:DNA Primers; D012333:RNA, Messenger