3-Hydroxybutyrate decreases noradrenaline affinity for rat erythrocyte ghost beta 2-adrenergic receptors.
The effects of physiological levels of 3-hydroxybutyrate on noradrenaline binding to rat erythrocyte membranes were studied. The binding of noradrenaline to membranes was determined by measuring the union of 3H-noradrenaline in the presence of varying concentrations of 3-hydroxybutyrate. Scatchard plots of the results were used to determine that 3-hydroxybutyrate provoked a loss of affinity of the beta 2-adrenergic receptors for adrenaline. Thus high (but physiological) 3-hydroxybutyrate levels can lower the binding of the catecholamine, thus diminishing the extent or intensity of adrenergic-driven responses.
Gómez
G
G
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Spain.
Cañas
X
X
Fernández-López
J A
JA
Remesar
X
X
Alemany
M
M
D020155:3-Hydroxybutyric Acid; D000818:Animals; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects / Q000378:metabolism*
D006885:Hydroxybutyrates; D018343:Receptors, Adrenergic, beta-2; D020155:3-Hydroxybutyric Acid; D009638:Norepinephrine