Evaluation of reformulated chemiluminescent DNA probe (AccuProbe) for culture identification of Mycobacterium kansasii.
A panel of 104 isolates belonging to the species Mycobacterium kansasii and 78 mycobacterial isolates belonging to other species was tested in parallel with the present commercially available DNA probe (AccuProbe; Gen-Probe) and with a new probe just developed by the same manufacturer. While the old version of the probe confirmed the previously reported low sensitivity (only 59% of the M. kansasii isolates reacted), the new one was 100% sensitive. Only two non-M. kansasii strains, both M. gastri isolates, gave false-positive hybridization results.
Microbiological and Virological Laboratory, Careggi Hospital, Florence, Italy. tortoli@dada.it
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Tortoli
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Simonetti
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Lavinia
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D009165:Mycobacterium Infections, Nontuberculous / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes