Moth-related antigens contained in the house dust of the Japanese house.
We established an enzyme-linked immunosorbent assay (ELISA) for determining moth-related antigens in house dust. Samples, biotinated anti-moth antibodies, and horseradish peroxidase labeled streptavidin were reacted with the microplate on which anti-moth rat polyclonal antibodies were absorbed. The concentration of moth antigens was correlated with absorbance in the range from 0.1 to 100 micrograms/ml. Cross-reaction with other insect antigens was observed; 10 micrograms of midge antigens and 100 micrograms of mosquito antigens corresponded to 1 microgram of the moth antigens, respectively. With this method, moth-related antigens can be detected in house dust. However, there was no remarkable difference in antigen levels among the three locations examined: a large city, a small town, and a rural area.
Department of Otorhinolaryngology, Nagoya City University Medical School, Japan.
S
S
Kitao
H
H
Ito
M
M
Ito
M
M
Suzuki
I
I
Takagi
S
S
Baba
N
N
Oota
D000818:Animals; D000906:Antibodies; D000941:Antigens; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007073:Immunoglobulin E; D007564:Japan; D009036:Moths; D051381:Rats
D000906:Antibodies; D000941:Antigens; D004391:Dust; D007073:Immunoglobulin E