Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3'-phosphotransferase type IIIa: role of substrate amino groups.
The interactions of the aminoglycoside 3'-phosphotransferase IIIa with aminoglycoside antibiotics lacking specific amino groups were examined by steady-state kinetic analyses. The results demonstrate that an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by this enzyme.
Department of Biochemistry, McMaster University, Hamilton, Ontario.
G A
GA
McKay
J
J
Roestamadji
S
S
Mobashery
G D
GD
Wright
D000588:Amines / Q000378:metabolism; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D016983:Enterococcus / Q000201:enzymology*; D019868:Kanamycin Kinase; D007700:Kinetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology*
D000588:Amines; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase