Human herpesvirus type 8 DNA sequences in cell-free plasma and mononuclear cells of Kaposi's sarcoma patients.
Human herpesvirus (HHV) type 8 has been detected in both classical and AIDS-related Kaposi's sarcoma, body-cavity lymphomas, and other types of tumors. HHV-8 has also been detected in DNA from peripheral blood mononuclear cells (PBMC) of some Kaposi's sarcoma patients and more readily in B cell fractions derived from panned cell subpopulations. Two patients were followed using several methods; in situ hybridization, solution-based polymerase chain reaction (PCR), and in situ PCR. HHV-8 was intermittently detected in plasma, and detection correlated with detection in PBMC. In situ PCR demonstrated HHV-8 sequences in both peripheral blood B lymphocytes and, to a lesser extent, T lymphocytes. HHV-8 may undergo periods of viremia while at other times it is undetectable and infects circulating B cells and some T cells.
Department of Medicine, University of Miami School of Medicine, Florida, USA.
W J
WJ
Harrington
O
O
Bagasra
C E
CE
Sosa
L E
LE
Bobroski
M
M
Baum
X L
XL
Wen
L
L
Cabral
G E
GE
Byrne
R J
RJ
Pomerantz
C
C
Wood
D000328:Adult; D001402:B-Lymphocytes / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*; D013601:T-Lymphocytes / Q000821:virology*; D014766:Viremia / Q000821:virology
D004279:DNA, Viral