A NMR study of parasitized Tenebrio molitor and Hymenolepis diminuta cysticercoids.
In vivo NMR spectra of uninfected and Hymenolepis diminuta-infected Tenebrio molitor fed D-(1-13C)glucose showed that infected beetles of both sexes had a significantly higher ratio for (glycogen C1/lipid (CH2)n) than the corresponding controls. Quantitative metabolic profiles and the per cent 13C-label in metabolites, based on NMR of perchloric acid extracts, are presented for control and infected beetles fed D-(1-13C)glucose and for H. diminuta cysticercoids. Female beetles, both control and infected, contained more glycogen than their male counterparts and infected beetles of both sexes possessed less glycerophos-phocholine, but more glycogen and a higher percentage label in glucose and trehalose than their respective controls. Label was also incorporated into glycogen, succinate, acetate, alanine and lactate. Extracts of cysticercoids from beetles fed D-(1-13C)glucose contained the following labelled compounds, in order of decreasing per cent 13C label: glucose, trehalose, alanine, succinate, lactate, glycogen and acetate. In vitro cultivation experiments, employing D-(1-13C)glucose, revealed that trehalose found in cysticercoids was of parasite, and not beetle, origin.
Department of Biology, University of Winnipeg, Manitoba, Canada.
J
J
Schoen
A
A
Modha
K
K
Maslow
M
M
Novak
B J
BJ
Blackburn
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002264:Carboxylic Acids / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006926:Hymenolepis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D012727:Sex Characteristics; D013712:Tenebrio / Q000378:metabolism* / Q000469:parasitology*; D014199:Trehalose / Q000378:metabolism
D000596:Amino Acids; D002264:Carboxylic Acids; D005997:Glycerylphosphorylcholine; D006003:Glycogen; D014199:Trehalose; D005947:Glucose