[Cloning, sequencing and characterizing repetitive DNA of Glycine max].
One satellite DNA family, cloned from Glycine max, was sequenced and chracterized. The unit of the repetitive sequences was 91bp. They concentrated on the arms of chromosomes M2 and M11, and spreaded on whole chromosomes Sm7 and M12. Southern blot analysis with 18 acessions, belonged to subgenus Glycine and subgenus Soj a of genus Glycine, showed that the repetitive sequences were specific to subgenus Soj a. It was subgenus specific repetitive sequence. This result, from another aspact, supported the idea that the three species in subgenus Soj a should be considered as one species.
Institute of Genetics Academia Sinica, Beijing.
D
D
Hui
F
F
Liu
S
S
Chen
J
J
Wang
Y
Y
Yan
Y
Y
Miao
D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*
D018744:DNA, Plant; D004276:DNA, Satellite