Long Ca2+ channel opening induced by large depolarization and Bay K 8644 in smooth muscle cells isolated from guinea-pig detrusor.
1. In smooth muscle cells enzymatically isolated from guinea-pig urinary bladder, Ca2+ channel currents were recorded by conventional cell-attached patch clamp techniques. In most recordings Bay K 8644 (2 microM) was contained in the patch pipette. 2. Closure of Ca2+ channels observed during the repolarizing steps was significantly slowed by preconditioning with large depolarizations (+80 and 100 mV), with or without Bay K 8644 in the pipette. 3. The sum of the unitary Ca2+ channel current traces obtained after large conditioning depolarizations (in the presence of Bay K 8644) showed a slowly deactivating tail current. 4. By use of this slow deactivating feature, the current-voltage relationship of the unitary Ca2+ channel current was continuously measured with a ramp pulse after large depolarization. The slope conductance ranged from 22 to 30 pS, compatible with that of L-type Ca2+ channels. 5. It is concluded that L-type Ca2+ channels in guinea-pig detrusor cells are open for much longer after large depolarizations consistent with their being two channel open states, and that Bay K 8644 prolongs the lifetime of both open states. The underlying mechanisms are discussed.
Department of Physiology, School of Medicine, Nagoya University, Japan.
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Nakayama
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Brading
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology*; D000818:Animals; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects*; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
D002120:Calcium Channel Agonists; D015220:Calcium Channels; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester