Cloning and functional expression of a cDNA encoding a mouse type 2 neuropeptide Y receptor.
A cDNA clone homologous with the human neuropeptide Y (NPY)-Y2 receptor has been isolated from a mouse brain cDNA library. Analysis of the predicted amino-acid sequence indicates that the polypeptide encoded by this cDNA is 94% homologous to the human NPY-Y2 receptor. In Chinese hamster ovary (CHO) cells expressing the mouse NPY-Y2 receptor, an increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were observed due to stimulation with NPY, NPY-(13-36) and peptide YY, but not with pancreatic polypeptide or [Leu31, Pro34]NPY. The fact that the NPY-induced increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were eliminated by pretreatment with pertussis toxin suggests that the NPY-Y2 receptor couples to PTX-sensitive G-protein(s), probably Gi/Go, in CHO cells.
Life Science Research Laboratory, Japan Tobacco Inc., Kanagawa, Japan. nakamura@tcrl.jti.co.jp
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Nakamura
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Aoki
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Hirano
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000378:metabolism / Q000494:pharmacology*; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D009478:Neuropeptide Y; D010455:Peptides; D017476:Receptors, Neuropeptide Y; D011994:Recombinant Proteins; C095889:neuropeptide Y2 receptor; D019894:Peptide YY; D005576:Colforsin; D000242:Cyclic AMP