Inhibition of antigen-specific IgE production by antigen coupled to membrane IgE peptide.
The membrane IgE peptide (MEP) encompassing 20 amino acids proximal to the C terminus of membrane IgE molecules, and secretory IgE peptides (SEP), spanning CH epsilon 1 to 4 domain were synthesized according to IgE genomic and cDNA sequences. Inhibition of anti-KLH and anti-BGG IgE, but not IgG responses was observed in mice treated with MEP-protein but not SEP-protein conjugates in complete/incomplete Freund's adjuvant. Only IgE responses directed toward proteins to which MEP was conjugated, were inhibited, while IgE responses to a concomitantly injected, unrelated antigen were not. Inhibition of antigen-specific IgE was also not correlated with levels of anti-MEP or anti-IgE antibodies, moreover, levels of total IgE remained comparable among mice treated with MEP-protein conjugates, native or glutaraldehyde-modified protein carriers. This observation may have significant import on future design of IgE immunotherapy. Treatment of MEP conjugated allergens prevents formation of IgE-anti-IgE complexes because the MEP sequence is absent from the secretory IgE.
Chen
S S
SS
Department of Veterinary and Biomedical Science, IANR, University of Nebraska-Lincoln, USA.
Schmaltz
R
R
Wang
Y Y
YY
Kong
Q X
QX
Petro
T
T
Li
Q
Q
Chang
T W
TW
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000378:metabolism / Q000494:pharmacology*; D002352:Carrier Proteins / Q000276:immunology / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000008:administration & dosage / Q000378:metabolism*
D000941:Antigens; D002352:Carrier Proteins; D000939:Epitopes; D010455:Peptides; D011947:Receptors, Antigen, B-Cell; D007073:Immunoglobulin E; D010047:Ovalbumin; D005620:Freund's Adjuvant