Aprotinin effects related to oxidative stress in cardiosurgery with mechanical cardiorespiratory support (CMCS).
There is evidence to support a relationship between oxidative stress and protease release in "ischemia-reperfusion damage." We have proposed that aprotinin may exert an antioxidant effect. A double blind clinical trial was performed with a control (G-1) and treated (G-2) groups, both submitted to CMCS. Blood samples were taken 5 times. Biochemical indicators were measured spectrophotometrically. Aprotinin was supplied by Bayer. Malonildialdehyde levels were greater in G-1 (7.2 +/- 3.6 nmoles/ml) than in G-2 (4 +/- 1.65) at the time of reperfusion. Phospholipase A2 exhibited a tendency of higher activity in G-1 than in G-2. Uric acid levels were higher in G-2 (431 +/- 274 mumoles/1) than in G-1 (224 +/- 188) at 5 minutes after aortic clamping, and catalase activity was greater in G-2 (294 +/- 55 KU/1) than in G-1 (118 +/- 47) at time of reperfusion. Low cardiac output was 10% in G-2 and 30% in G-1. Arrythmias appeared in 30% of G-2 and in 60% of G-1. These results suggest an antioxidant effect of aprotinin under ischemia-reperfusion conditions.
Broche
V F
VF
Biomedical Research Center Victoria de Giròn, Havana, Cuba. jcarlos@girón.sld.cu
Suàrez
A R
AR
Olembe
E
E
Fernàndez
G E
GE
Céspedes
E M
EM
Garcia
J C
JC
Reynoso
E
E
Nùñez
P
P
Prieto
E
E
D007611:Aprotinin / Q000627:therapeutic use*; D006348:Cardiac Surgical Procedures / Q000009:adverse effects*; D004311:Double-Blind Method; D006801:Humans; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D018384:Oxidative Stress
D007611:Aprotinin