Cyclo (LL-dimethionine) is not utilized by rats as a methionine source.
A low casein diet supplemented with 0.2-0.3% L-methionine promotes growth in rats, although it causes liver fat accumulation. Oligo-L-methionine on the other hand, prepared by papain-catalyzed oligomerization of L-methionine ethylester, stimulates growth in rats without causing liver fat accumulation, when added to a 8% casein diet. Oligo-L-methionine is a mixture of oligomers, with a oligomerization degree of 5 to 12, and is hydrolyzed very slowly by pancreatic juice and everted gut rings of rats. A methionine derivative, cyclo (LL-dimethionine), was examined in this study as it is hydrolyzed and/or absorbed very slowly from the intestinal tract and it was expected to be utilized as a methionine source without causing liver fat accumulation, when added to a low casein diet. It consists of two methionine residues linked together by two cis-peptide bonds and, therefore, is expected to be hydrolyzed and/or absorbed very slowly from intestinal tract. However, although cyclo (LL-dimethionine) was absorbed slowly from the intestinal tract, it was not utilized by rats as a methionine source, when added to 8% casein.
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
T
T
Kasai
A
A
Fujii
T
T
Hashido
S
S
Kiriyama
D000818:Animals; D002364:Caseins / Q000008:administration & dosage; D004032:Diet; D006868:Hydrolysis; D007408:Intestinal Absorption; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000378:metabolism*; D009753:Nutritive Value; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
D002364:Caseins; D010456:Peptides, Cyclic; C104132:cyclo-dimethionine; D008715:Methionine