Detection of Salmonella DNA in chicken embryos and environmental samples by polymerase chain reaction.
By polymerase chain reaction (PCR) using a pair of primers specific for Salmonella phoE gene a 365-bp specific gene fragment could be amplified from yolk of infertile eggs and dead-in-shell chicken embryos, and from environmental samples. Out of 45 dead-in-shell embryo samples, 20 (44.4%) were found positive for Salmonella DNA by PCR compared to 11 (24.4%) by bacteria isolation. Salmonella DNA could also be detected from infertile eggs, chicken faeces, floor litter and chick fluff, which incidence was higher than that by bacteria isolation.
School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
L M
LM
Tuchili
H
H
Kodama
R N
RN
Sharma
I
I
Takatori
G S
GS
Pandey
S
S
Kabilika
M
M
Mukamoto
S
S
Tsuji
T
T
Baba
D000818:Animals; D002642:Chick Embryo / Q000737:chemistry*; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005243:Feces / Q000737:chemistry; D015994:Incidence; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology; D012475:Salmonella / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000453:epidemiology
D004269:DNA, Bacterial