The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils.
Monoclinic calcium pyrophosphate dihydrate (m-CPPD) crystals were synthesized and characterized using physical methods, IgG binding to m-CPPD crystals was quantitated, and the effect of IgG or plasma opsonization on m-CPPD-induced neutrophil activation was determined. Adsorption of IgG to crystals was measured using fluorescent-labelled FITC-IgG. Neutrophil activation by uncoated m-CPPD and crystals precoated with IgG or plasma was measured using luminol-enhanced chemiluminescence, superoxide anion generation, and myeloperoxidase release. m-CPPD bound small (compared to triclinic CPPD) but significant amounts of IgG and induced a strong activation of neutrophils at low concentrations of crystals. The rate and extent of chemiluminescence, superoxide anion production, and degranulation was not affected by precoating m-CPPD crystals with IgG during the early phase of neutrophil responses, but was inhibited by the precoating of crystals with plasma.
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
C I
CI
Winternitz
J K
JK
Jackson
H M
HM
Burt
D000327:Adsorption; D002131:Calcium Pyrophosphate / Q000097:blood* / Q000737:chemistry* / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D003460:Crystallization; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010316:Particle Size; D011485:Protein Binding; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D014961:X-Ray Diffraction
D007074:Immunoglobulin G; D013481:Superoxides; D002131:Calcium Pyrophosphate