Suppression of fibroblast cell growth by overexpression of LIM-kinase 1.
LIM-kinase 1 (LIMK1) is a serine/threonine kinase containing two LIM motifs at the N-terminus. The functional role of LIMK1 has remained unknown. In this study, we examined the role of LIMK1 in cell growth of fibroblasts. Induced expression of LIMK1 in NIH3T3 cells led to growth retardation. Transfection of LIMK1 sense cDNA into NIH3T3 and H-ras-transformed FYJ10 fibroblasts significantly suppressed colony formation of these cells. In contrast, transfection with LIMK1 antisense cDNA strongly stimulated colony formation of the NIH3T3 cells. These findings suggest that LIMK1 functions as a negative regulator of fibroblast cell growth, and may play a role in tumor suppression.
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
O
O
Higuchi
G H
GH
Baeg
T
T
Akiyama
K
K
Mizuno
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D011905:Genes, ras; D054461:Lim Kinases; D051379:Mice; D008845:Microinjections; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D014162:Transfection
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011494:Protein Kinases; D054461:Lim Kinases; C515893:Limk1 protein, mouse; C515894:Limk1 protein, rat; D017346:Protein Serine-Threonine Kinases