Effects of methylglyoxal bis (cyclopentylamidinohydrazone) (MGBCP) on cell cycle progression in three human osteosarcoma cell lines.
Our previous experiments have indicated that the antitumor effects of a polyamine biosynthetic inhibitor, methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), on human osteosarcoma cell lines such as MG-63, G-292 and HOS cells are obtained by its action depleting the cellular polyamine contents. In the present study, the effects of polyamine depletion by MGBCP on the cell cycle progression in these osteosarcoma cell lines were investigated by flow cytofluormetric analysis. MGBCP arrested the tumor cells at the G1 phase by preventing the G/S phase transition. Mitotic indexes (MIs) in these MGBCP-inhibited tumor cells were also decreased. These findings suggest that MGBCP suppresses the cell cycle progression in osteosarcoma MG-63, G-292 and HOS cells by inhibiting intracellular polyamine biosynthesis.
Department of Orthopedic Surgery, College of Medical Sciences, Mie University, Japan.
K
K
Mori
H
H
Hibasami
J
J
Sonoda
S
S
Fujinami
S
S
Sekiguchi
M
M
Tajima
T
T
Yamazaki
S
S
Higuchi
A
A
Uchida
K
K
Nakashima
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D008935:Mitoguazone / Q000031:analogs & derivatives* / Q000494:pharmacology; D008940:Mitotic Index; D012516:Osteosarcoma / Q000473:pathology; D016196:S Phase / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C059234:methylglyoxal bis(cyclopentylamidinohydrazone); D008935:Mitoguazone