Isolation of a Candida glabrata centromere and its use in construction of plasmid vectors.
A centromere has been isolated from Candida glabrata by functional selection based on the lethality of the SUP11 gene at high copy number. Nucleotide sequence analysis revealed a centromeric structure similar to that of Saccharomyces cerevisiae: the two highly conserved elements CDEI (8 bp) and CDEIII (26 bp) are separated by a 79-bp A+T-rich element, CDEII. Three centromere-bearing plasmid vectors with different selection markers have been constructed. These plasmids were highly stable in mitosis (< 1% loss rate per generation) and exist in one or two copies per cell.
Kitada
K
K
Department of Mycology, Nippon Roche Research Center, Kanagawa, Japan. kunio.kitada@roche.com
Yamaguchi
E
E
Arisawa
M
M
D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000472:pathogenicity; D002503:Centromere / Q000235:genetics*; D017124:Conserved Sequence; D004271:DNA, Fungal / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal