Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma.
To investigate a possible phenotypic modulation, MT-8L and MT-9L cells were induced by in vitro culture of undifferentiated MT-8 and fibrohistiocytic MT-9 cells, which had been established from a rat malignant fibrous histiocytoma (MFH), in the medium containing 10 micrograms lipopolysaccharide (LPS)/ml. MT-8L and MT-9L gave greater positive reactions for histiocytic lysosomal markers and showed ultrastructures of histiocytic natures. In MT-8L, alpha-smooth muscle actin-positive myofibroblastic cells also significantly increased in number. MT-8L expressed both histiocytic and myofibroblastic phenotypes. MT-8L-induced tumors consisted mainly of storiform type MFH, differing from undifferentiated sarcoma type induced by MT-8. MT-9L and MT-9 tumors showed a storiform pattern. A phenotypic modulation of MFH cells was easily induced by LPS treatment.
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
J
J
Yamate
M
M
Tajima
K
K
Shibuya
M
M
Kuwamura
T
T
Kotani
S
S
Sakuma
D000818:Animals; D003470:Culture Media; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology* / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009368:Neoplasm Transplantation; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
D003470:Culture Media; D008070:Lipopolysaccharides