Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes.
A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication.
Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.
G
G
Wallukat
H U
HU
Simon
W D
WD
Müller
I
I
Wolf
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002478:Cells, Cultured; D003429:Cross Reactions; D004074:Digitoxin / Q000276:immunology / Q000494:pharmacology; D004077:Digoxin / Q000276:immunology* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006027:Glycosides / Q000276:immunology; D006321:Heart / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats
D000911:Antibodies, Monoclonal; D006027:Glycosides; D010042:Ouabain; D004077:Digoxin; D004074:Digitoxin