Calcium-cadmium interaction on sugar absorption across the rabbit jejunum.
The element Cd is considered to have no biological function and is highly toxic to humans and animals. Toxic effects of this metal upon cell membrane structure and function have been shown. On the other hand, Ca is an essential element in a wide variety of cellular activities. The present study was initiated to research whether the interaction between Ca and Cd could affect D-galactose absorption across the rabbit jejunum in vitro. In media with Ca2+, when CdCl2 was present at 0.5 or 1 mM, Cd was found to significantly reduce the sugar absorption. In Ca2+ -free media, where CaCl2, was omitted and replaced isotonically with choline chloride, the sugar transport was not modified by Cd, but when CaCl2 was replaced isotonically with MgCl2, the inhibition is observed. Verapamil at 10(-6)M (blocking mainly Ca2+ transport) did not modify the inhibitory effect of cadmium on D-galactose transport. When 10(-6)M of A 23187 (Ca2+ specific ionophore) was added in media with/without Ca2+; CdCl2 produced no change in D-galactose transport. These results suggest that Ca and Cd could have affinity for the same chemical groups of enterocyte membrane, which would be related with the intestinal absorption of D-galactose.
Mesonero
J E
JE
Fisiologia, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Yoldi
M C
MC
Yoldi
M J
MJ
D000704:Analysis of Variance; D000818:Animals; D019256:Cadmium Chloride / Q000378:metabolism / Q000633:toxicity*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D002794:Choline / Q000378:metabolism / Q000633:toxicity; D004347:Drug Interactions; D004848:Epithelium / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018697:Nootropic Agents / Q000378:metabolism / Q000633:toxicity; D011817:Rabbits; D013237:Stereoisomerism; D014700:Verapamil / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D002121:Calcium Channel Blockers; D002273:Carcinogens; D018697:Nootropic Agents; D014867:Water; D000001:Calcimycin; D014700:Verapamil; D019256:Cadmium Chloride; D002794:Choline; D002118:Calcium; D005690:Galactose