Observation on the nucleic acids in the chlamydospores of Candida albicans.
The presence of red (RNA) and green (DNA) fluorescent material identifying nucleic acids in the chlamydospores of Candida albicans has been well documented. Red fluorescence in chlamydospores is relatively diffused and ranges from small spots, observed in hyphal cells, to the entire protoplasmic content. Green fluorescence is rarely visible in these structures and, when present it can be observed next to the plasmalemma. The initial percentage values of the two curves related to the cell counts of red fluorescence of the suspensor cells and chlamydospores showed remarkable differences between the two structures. About 54% of the chlamydospores showed red fluorescence compared to about 28% of the suspensor cells. It seems from the results obtained in this study that much RNA was produced and/or accumulated in the chlamydospores and suspensor cells, rather than in mycelium where red fluorescence was not observed. The results obtained sustain the hypothesis that a chlamydospore should he considered a fully functional cell that is morphologically and physiologically active and independent from mycelium.
Vidotto
V
V
Dipartimento discipline Medico-Chirurgiche, sez. Malattie Infettive, Università di Torino, Italy.
Bruatto
M
M
Accattatis
G
G
Caramello
S
S
D000165:Acridine Orange; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D004271:DNA, Fungal / Q000378:metabolism; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D009696:Nucleic Acids / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D013172:Spores, Fungal / Q000187:drug effects / Q000378:metabolism
D004271:DNA, Fungal; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012331:RNA, Fungal; D003513:Cycloheximide; D000165:Acridine Orange