Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography.
This work describes the application of the fully automated high-performance liquid chromatographic system to the analysis of PCR-amplified products. Efficient separations of both DNA restriction fragments and PCR products were performed using an anion-exchange DEAE-NPR column, packed with 2.5-microns nonporous particles. The automated HPLC method was employed for the separation, quantitation, and purification of PCR products in less than 10 min in a single step.
Perkin-Elmer Corporation, Norwalk, CT 06859-0259, USA.
E D
ED
Katz
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA