Inhibition of tuberoinfundibular dopaminergic neural activity during suckling: involvement of mu and kappa opiate receptor subtypes.
Previous studies have shown that mu (mu) and kappa (kappa) opioid antagonists inhibit suckling-induced prolactin release. Prolactin responses elicited by pup suckling or opioid administration are mediated, at least in part, by suppression of dopamine (DA) release from tuberoinfundibular dopaminergic (TIDA) neurons in the hypothalamus. We examined the effects of the mu opiate receptor antagonist, beta-funaltrexamine (beta-FNA), and the kappa opiate receptor antagonist, nor-binaltorphimine (nor-BNI) on the activity of TIDA neurons in lactating rats. TIDA neuronal activity was determined by measuring DOPA accumulation in the caudate putamen (CP) and median eminence (ME). The effects of opioid antagonist treatment were determined in pup-deprived (low circulating prolactin levels) or pup-suckled rats (high circulating prolactin levels). The accumulation of 5-hydroxytryptophan (5-HTP) in the medial preoptic area (MPOA), the anterior hypothalamus (AH) and the median eminence (ME) was quantified as an index of serotonergic activity in the same animals for comparative purposes. In vehicle treated rats, suckling caused a significant and selective decrease in DOPA accumulation in the ME. beta-FNA (5 micrograms, i.c.v.) pretreatment significantly increased DOPA accumulation in the ME of pup-deprived and pup-suckled rats. beta-FNA pretreatment also prevented the suckling-induced suppression of DOPA accumulation in the ME. In contrast to the actions of beta-FNA, pretreatment with nor-BNI (8 micrograms, i.c.v.) did not significantly affect the activity of the TIDA neurons in pup-deprived or pup-suckled rats. Suckling alone did not alter 5-HTP accumulation in any of the brain regions examined, and neither opioid antagonist had appreciable effects on 5-HTP accumulation. These results demonstrate that the EOP tonically inhibit the TIDA neurons in both pup-deprived and pup-suckled, post-partum female rats by acting through the mu, but not the kappa, opiate receptor subtype. Furthermore, the suckling-induced inhibition of TIDA neurons is also mediated through the EOP acting at mu, but not kappa opioid receptors.
Callahan
P
P
Department of Zoology, Miami University, Oxford, OH 45056, USA.
Baumann
M H
MH
Rabii
J
J
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D002421:Caudate Nucleus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004298:Dopamine / Q000502:physiology*; D005260:Female; D007032:Hypothalamus, Anterior / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D008297:Male; D008473:Median Eminence / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011301:Preoptic Area / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017473:Receptors, Opioid, kappa / Q000037:antagonists & inhibitors* / Q000502:physiology*; D017450:Receptors, Opioid, mu / Q000037:antagonists & inhibitors* / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism / Q000502:physiology; D013391:Sucking Behavior / Q000502:physiology
D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; C051843:binaltorphimine; D012701:Serotonin; D009271:Naltrexone; D004295:Dihydroxyphenylalanine; C024524:beta-funaltrexamine; D011388:Prolactin; D006916:5-Hydroxytryptophan; D004298:Dopamine