Effects of propionate and ammonium on contractions produced by sodium removal in human internal mammary artery and saphenous vein.
Effects of propionate and ammonium on contractions produced by Na+ removal (0-Na+ contraction, replaced with N-methyl-D-glucamine) were studied and compared with those produced by 60 mM K+ in muscle strips isolated from human internal mammary artery (IMA) and saphenous vein (SV), clinically used as bypass vessels. Contractions induced by 60 mM K+ (60-K+ contractions) were similar in both vessels. 0-Na+ contraction was significantly larger in the vein than in the artery. Neither 60-K+ nor 0-Na+ contractions were significantly affected by NG-nitro-L-arginine (30 microM) or phenoxybenzamine (1 microM). Ouabain (2 microM) slowly potentiated 0-Na+ contractions and the 3rd contraction produced 60 min after ouabain application was 291 +/- 36% in the artery and 184 +/- 41% of the control (n = 10) in the vein. Propionate (20 mM) potentiated 60-K+ contraction more strongly in the vein than in the artery, but potentiation of 0-Na+ contraction was greater in the artery than in the vein. On the other hand, ammonium (20 mM) inhibited 60-K+ contractions more strongly in the artery than the vein, whereas inhibition of 0-Na+ contraction was stronger in the vein. 0-Na+ contraction is likely to be produced by an Na(+)-Ca2+ exchange mechanism, and the effects of propionate and ammonium are considered to be mediated by intracellular acidification and alkalinization, respectively. It is speculated that an Na(+)-H+ exchange may also be involved in 0-Na+ contraction and that SV has slightly lower pHi than IMA.
Miyahara
K
K
Department of Thoracic Surgery, School of Medicine, Nagoya University, Japan.
Murase
M
M
Tomita
T
T
D000328:Adult; D000368:Aged; D000643:Ammonium Chloride / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008323:Mammary Arteries / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology*; D012501:Saphenous Vein / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000172:deficiency*; D014661:Vasoconstriction / Q000187:drug effects*
D011422:Propionates; D000643:Ammonium Chloride; D010042:Ouabain; D012964:Sodium; D011188:Potassium