Extremely preterm infants (< 28 weeks) are capable of gluconeogenesis from glycerol on their first day of life.
Extremely preterm infants have been shown capable of producing glucose at a rate comparable to that of term infants, but virtually no data are available on their capacity for lipolysis and gluconeogenesis. To address this issue, we studied the flux of glycerol and its gluconeogenic contribution to hepatic glucose output by determining the endogenous plasma appearance rate of glycerol (glycerol Ra) and its conversion to glucose in 10 newborn infants, 24-27 wk of gestational age. The study was performed during the 1st d of life by tracer dilution technique using [6,6-2H2]glucose and [2-13C]glycerol given as constant rate i.v. infusions. Plasma isotopic enrichments of the tracers were obtained by gas chromatography/mass spectrometry. Endogenous glycerol Ra ranged from 2.4 to 21.6 (median 5.0) mumol.kg-1.min-1, of which 31.5% (25.6-64.4%) was converted to glucose. The glucose production rate averaged 17.5 +/- 5.4 mumol.kg-1.min-1 (3.2 +/- 1.0 mg.kg-1.min-1), of which 5.0% (1.6-37.6%) was derived from glycerol. The results show that extremely preterm infants, despite limited fat stores, are capable of generating glycerol at a rate within the range reported for term and near term newborns. The infants were also capable of converting part of this glycerol to glucose, providing a contribution to hepatic glucose production comparable to that found in more mature newborns.
Sunehag
A
A
Uppsala University Children's Hospital, Sweden.
Ewald
U
U
Gustafsson
J
J
D001724:Birth Weight; D002247:Carbon Isotopes; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D005865:Gestational Age; D005934:Glucagon / Q000097:blood; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007553:Isotope Labeling / Q000379:methods; D008099:Liver / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D013972:Thyrotropin / Q000097:blood; D014316:Tritium
D002247:Carbon Isotopes; D007328:Insulin; D014316:Tritium; D013972:Thyrotropin; D005934:Glucagon; D003903:Deuterium; D005947:Glucose; D005990:Glycerol