Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
Howard Hughes Medical Institute, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.
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D003001:Cloning, Molecular; D004274:DNA, Recombinant; D015723:Gene Library; D005816:Genetic Complementation Test; D005819:Genetic Markers; D014644:Genetic Variation; D005822:Genetic Vectors / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D010957:Plasmids; D012568:Schizosaccharomyces / Q000235:genetics*
D004274:DNA, Recombinant; D005819:Genetic Markers; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase