Potential gene therapy for lecithin-cholesterol acyltransferase (LCAT)-deficient and hypoalphalipoproteinemic patients with adenovirus-mediated transfer of human LCAT gene.
BACKGROUND
Overexpression of human lecithin-cholesterol acyltransferase (LCAT) in transgenic mice results in an increase of the antiatherogenic HDLs.


RESULTS
To investigate the potential use of LCAT for gene therapy, a recombinant adenovirus was constructed in which the human LCAT cDNA was expressed under the control of the human cytomegalovirus immediate/early promoter followed by a chimeric intron (AdCMV human LCAT). Human apolipoprotein (apo) A-I transgenic mice infected with AdCMV human LCAT by intravenous injection accumulated reactive LCAT in the plasma. LCAT activity was increased 201-fold in the plasma of mice infected with 1 x 10(6) pfu AdCMV human LCAT, from 45 +/- 2 to 9068 +/- 812 nmol.mL-1.h-1, in comparison with basal LCAT activity measured in control mice, 5 days after injection. Plasma HDL cholesterol levels rose from 117 +/- 12 to 797 +/- 48 mg/dL, and plasma human apo A-I concentrations increased from 247 +/- 14 to 616 +/- 17 mg/dL, in AdCMV human LCAT infected mice compared with control mice. HDL particles were larger and had a different electrophoretic mobility. Studies of cholesterol efflux by incubation of serum with cholesterol-loaded Fu5AH cells showed that serum from AdCMV human LCAT-infected mice promoted a significantly higher efflux than did that of the controls.


CONCLUSIONS
These data establish the potential of this approach for treatment of subjects with LCAT gene defects as well as patients with low plasma levels of apo A-I and HDL cholesterol.
Séguret-Macé
S
S
Rhône-Poulenc Rorer, Gencell Division, Centre de recherche de Vitry-Alfortville, Vitry sur Seine, France.
Latta-Mahieu
M
M
Castro
G
G
Luc
G
G
Fruchart
J C
JC
Rubin
E
E
Denèfle
P
P
Duverger
N
N
D000256:Adenoviridae; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood / Q000235:genetics; D006528:Carcinoma, Hepatocellular; D002788:Cholesterol Esters / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D004586:Electrophoresis; D005227:Fatty Acids / Q000032:analysis; D015870:Gene Expression / Q000502:physiology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000235:genetics*; D010743:Phospholipids / Q000097:blood / Q000737:chemistry; D013631:Tangier Disease / Q000235:genetics*; D014280:Triglycerides / Q000097:blood; D014407:Tumor Cells, Cultured / Q000378:metabolism
D016632:Apolipoprotein A-I; D002788:Cholesterol Esters; D008076:Cholesterol, HDL; D005227:Fatty Acids; D008074:Lipoproteins; D010743:Phospholipids; D014280:Triglycerides; D007862:Phosphatidylcholine-Sterol O-Acyltransferase