Uptake of indium-111-anti-intercellular adhesion molecule-1 monoclonal antibody in the allografted rat lung during acute rejection.
BACKGROUND
Although many methods for detection and quantification of allograft rejection of the lung have been explored, only histologic diagnosis by lung biopsies has gained widespread acceptance. To examine whether indium-111-anti-intercellular adhesion molecule-1 monoclonal antibody imaging can noninvasively detect acute lung rejection, we measured the uptake of this radiopharmaceutical in lung tissue and with scintigraphy in orthotopically transplanted rat lungs.


METHODS
The left lung transplant model was used with Lewis- to Wistar-King rat allografts and Lewis isograft controls. Lungs were harvested 2,3,4,5,6, and 7 days after transplantation. The transplanted and native lungs were removed 24 hours after injection of the radiotracer, weighed, and counted in a gamma well counter; uptake ratios of the transplanted or native lungs were then calculated, and scintigraphy was performed.


RESULTS
Histologic rejection scores by the grading system of the International Society for Heart and Lung Transplantation at 2,3,4,5,6, and 7 in the allografts were 1.2 +/- 0.2, 2.3 +/- 0.6, 3.0 +/- 0.5, 3.7 +/- 0.4, and 4, and 4, respectively. The uptake ratios of the allografts 3,4, and 5 days after transplantation were significantly higher than the values of the respective isografts and correlated with histologic rejection grades. However, 6 days after transplantation, uptake ratios of allografts decreased and did not correlate with histologic grades. On days 3,4, and 5 after transplantation, the tracer uptake within the allografts was visualized by means of scintigraphy.


CONCLUSIONS
We conclude that indium-111-anti-intercellular adhesion molecule-1 monoclonal antibody increased during mild to moderate acute lung rejection. An abnormal scintigram with this radiotracer suggests that lung biopsy should be performed to exclude lung rejection.
Amano
J
J
Department of Cardiothoracic Surgery, Tokyo Women's Medical College, Japan.
Hiroe
M
M
Ohta
Y
Y
Ishiyama
S
S
Nishikawa
T
T
Tanaka
H
H
Sunamori
M
M
Suzuki
A
A
Miyasaka
M
M
Marumo
F
F
D000818:Animals; D006084:Graft Rejection / Q000000981:diagnostic imaging* / Q000276:immunology; D007205:Indium Radioisotopes; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008168:Lung / Q000000981:diagnostic imaging; D016040:Lung Transplantation / Q000000981:diagnostic imaging / Q000276:immunology*; D008297:Male; D016719:Radioimmunodetection; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D007205:Indium Radioisotopes; D018799:Intercellular Adhesion Molecule-1