Synchronized induction in bFGF and Cdk2 during gastric ulcer healing.
The processes of gastric mucosal repair are regulated by growth factors triggering the signals for cell cycle progression. We examined the expression of gastric mucosal basic fibroblast factor (bFGF) and cyclin dependent kinase (p34Cdk2) at different stages of chronic ulcer healing. The results of assays revealed that ulcer healing was accompanied by an increase in mucosal expression of bFGF and Cdk2. A significant induction in bFGF and Cdk2 occurred by the 2nd day of ulcer healing, reached the respective maximums of 2.3- and 2.2-fold increase by the 4th day of healing, and remained still elevated (20%) on the 14th day when the ulcer was essentially healed. The results demonstrate a synchronized induction in bFGF and cell cycle regulatory kinase, Cdk2, with ulcer healing.
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400, USA.
B L
BL
Slomiany
J
J
Piotrowski
A
A
Slomiany
D019342:Acetic Acid; D000818:Animals; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000166:cytology / Q000473:pathology* / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D014945:Wound Healing
D016222:Fibroblast Growth Factor 2; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D019342:Acetic Acid