Nerve growth factor circulating levels are increased in Kawasaki disease: correlation with disease activity and reduced angiotensin converting enzyme levels.
OBJECTIVE
To investigate the serum levels of nerve growth factor (NGF) and their possible relationship with disease activity in Kawasaki disease.


METHODS
Twelve children with Kawasaki disease, 12 healthy children (afebrile controls), and 12 children with high fever related to upper respiratory tract viral infections (URTVI) were enrolled in the study. Serum NGF levels were measured by an ELISA method before and after therapy. In addition, angiotensin converting enzyme (ACE), and erythrocyte sedimentation rate (ESR), complete hemochrome, and C-reactive protein were tested as indicators of endothelial injury and disease activity, respectively.


RESULTS
NGF levels were strikingly increased in the acute phase of Kawasaki disease (1219.54 +/- 1660.29 pg/ml) and decreased in the inactive phase of the disease (277.08 +/- 245.64 pg/ml), while remaining significantly higher than controls (6.5 +/- 2.03 pg/ml). In febrile patients with URTVI, NGF levels (30.18 +/- 44.70 pg/ml) were moderately but significantly increased compared to afebrile controls, but remarkably lower in respect to active and inactive Kawasaki disease. In active Kawasaki disease, ACE values were significantly lower than in healthy children (2.8 +/- 1.7 vs 10.1 +/- 4.2 pmol/ml/min; p < 0.001) and increased during the inactive phase, but remaining lower than in controls (4.8 +/- 2.7 pmol/ml/min); ESR, hemoglobin level, and platelet count showed significant correlation with NGF, while ACE levels showed a significant inverse correlation with ESR and NGF.


CONCLUSIONS
In Kawasaki disease, NGF increased together with decreased ACE may be linked to a diffuse vascular inflammatory process. NGF and ACE levels remained abnormal even when there was no clinical sign of disease activity. This may indicate that the disease process is not in complete remission and suggests careful and prolonged cardiac followup until their normalization.
Department of Pediatrics, University of Florence, Italy.
F
F
Falcini
M M
MM
Cerinic
M
M
Ermini
S
S
Generini
A
A
Lombardi
A
A
Pignone
G
G
Leoncini
P
P
Tirassa
L
L
Aloe
D002675:Child, Preschool; D005260:Female; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology; D007223:Infant; D008297:Male; D008954:Models, Biological; D009080:Mucocutaneous Lymph Node Syndrome / Q000097:blood* / Q000188:drug therapy; D009414:Nerve Growth Factors / Q000097:blood*; D007703:Peptidyl-Dipeptidase A / Q000097:blood* / Q000187:drug effects; D012141:Respiratory Tract Infections / Q000097:blood
D016756:Immunoglobulins, Intravenous; D009414:Nerve Growth Factors; D007703:Peptidyl-Dipeptidase A