Flux control exerted by overt carnitine palmitoyltransferase over palmitoyl-CoA oxidation and ketogenesis is lower in suckling than in adult rats.
We examined the potential of overt carnitine palmitoyltransferase (CPT I) to control the hepatic catabolism of palmitoyl-CoA in suckling and adult rats, using a conceptually simplified model of fatty acid oxidation and ketogenesis. By applying top-down control analysis, we quantified the control exerted by CPT I over total carbon flux from palmitoyl-CoA to ketone bodies and carbon dioxide. Our results show that in both suckling and adult rat, CPT I exerts very significant control over the pathways under investigation. However, under the sets of conditions we studied, less control is exerted by CPT I over total carbon flux in mitochondria isolated from suckling rats than in those isolated from adult rats. Furthermore the flux control coefficient of CPT I changes with malonyl-CoA concentration and ATP turnover rate.
Department of Biochemistry, University of Cambridge, U.K.
S
S
Krauss
C V
CV
Lascelles
V A
VA
Zammit
P A
PA
Quant
D000818:Animals; D000831:Animals, Newborn; D002244:Carbon / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D005260:Female; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D010171:Palmitoyl Coenzyme A; D002244:Carbon; D002334:Carnitine O-Palmitoyltransferase