The effect of AZT on dendritic cell number and provirus load in the peripheral blood of AIDS patients: a preliminary study.
In this pilot study, the numbers of dendritic cells (DC) in peripheral blood of AIDS patients and the level of infection with HIV1 were determined before and after AZT treatment. Mononuclear cells were cultured overnight and DC were identified by their lack of labelling with antibodies specific for T, B and natural killer (NK) cells and monocytes and by their high level of staining with antibodies for MHC class II molecules. Although the numbers of DC identified by this method were lower than those identified morphologically in earlier studies (Macatonia et al., 1990), the numbers in three untreated AIDS patients were below the range seen in normals. There was also a marked rise in DC number in patients given AZT therapy. In two patients, there was a significant provirus load in the DCs which was decreased two to three weeks after the commencement of AZT therapy. The studies suggest that DC numbers and their infection levels may be markers of disease in HIV infection.
Patterson
S
S
Imperial College School of Medicine at Northwick Park Institute for Medical Research, Harrow, Middlesex.
Helbert
M
M
English
N R
NR
Pinching
A J
AJ
Knight
S C
SC
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000821:virology; D005434:Flow Cytometry; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008969:Molecular Sequence Data; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000187:drug effects* / Q000254:growth & development; D015215:Zidovudine / Q000494:pharmacology* / Q000627:therapeutic use
D000998:Antiviral Agents; D015215:Zidovudine