Analysis of DNA flanking the treA gene of Bacillus subtilis reveals genes encoding a putative specific enzyme IITre and a potential regulator of the trehalose operon.
Nucleotide sequencing revealed the genes treP encoding a putative specific enzyme IITre upstream from treA and treR encoding a potential regulator downstream from treA of Bacillus subtilis 168. The treP gene encodes a 470-amino acid (aa) protein (50 kDa) showing high similarities to several different specific enzymes II of phosphoenolpyruvate-dependent phosphotransferase systems. treR encodes a 238-aa protein (28 kDa) with high homologies in its N-terminal part to DNA-binding proteins including a helixturn-helix motif. Homologies in its C-terminal part place it in the family of FadR-GntR transcriptional regulators. The three genes, treP-treA-treR, are probably organized in one operon expressed by a sigma A-dependent promoter 53 bp upstream from treP and a rho-independent terminator 28 bp downstream from treR.
Schöck
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Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.
Dahl
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D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004186:Disaccharidases / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D014199:Trehalose / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; C094584:trehalose repressor protein, bacteria; D014199:Trehalose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C094583:phosphoenolpyruvate-trehalose phosphotransferase, bacteria; D004186:Disaccharidases; C040631:alpha, alpha-phosphotrehalase