Identification of cis-acting elements as DNase I hypersensitive sites in lysozyme gene chromatin.
DNase I hypersensitive sites in chromatin of eukaryotic cells mark the positions of multifactorial cis-acting elements. Mapping DH sites by indirect end labeling is a convenient procedure used for identifying regulatory elements within extensive regions of chromatin and for gaining information about their functional specificity as well as their fine structure.
Sippel
A E
AE
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Germany.
Saueressig
H
H
Huber
M C
MC
Hoefer
H C
HC
Stief
A
A
Borgmeyer
U
U
Bonifer
C
C
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002645:Chickens; D002843:Chromatin / Q000235:genetics* / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D003850:Deoxyribonuclease I; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology; D007202:Indicators and Reagents; D009000:Monocytes / Q000166:cytology / Q000201:enzymology; D009113:Muramidase / Q000096:biosynthesis* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping / Q000379:methods; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis
D002843:Chromatin; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D009113:Muramidase; D001616:beta-Galactosidase