Inverse PCR amplification of low-abundancy message of gamma delta T cell receptor genes.
The technique of inverse PCR permits the rapid amplification and identification of unknown DNA segments adjacent to well characterized core regions. In the field of immunology anchored PCR and inverse PCR are useful methods for examining junctional diversity and unknown variable gene segments of rearranged T cell receptor genes. We have applied an improved inverse PCR protocol to study the repertoire of gamma delta T cell receptor genes in the developing thymus of the mouse.
Department of Immunology, Paul Ehrlich Institute, Langen, Germany.
J
J
Weber-Arden
O M
OM
Wilbert
D
D
Kabelitz
B
B
Arden
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002021:Buffers; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*
D002021:Buffers; D018076:DNA, Complementary; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta