A segment corresponding to amino acids Gln199-Lys208 of murine IL-1 alpha cross-reacts with an antigenic determinant localized in the Z-line of Drosophila melanogaster myofibrils.
Immunofluorescence microscopic observations indicated that a monoclonal antibody, Vmp 18, raised against the peptide 199-208 of murine interleukin 1 alpha, cross-reacted with an antigenic determinant of Drosophila thorax muscles. Immunoelectron microscopic analysis showed that the gold particles were mainly localized in the Z-line which is the attachment site of thin filaments from adjacent sarcomeres. On the contrary, the antibody failed to mark the Z-line in vertebrate skeletal muscle. A Western blot of total protein extract from Drosophila thorax muscles bound a protein of 43 kDa. Our observations suggest that the Vmp 18 antibody could contribute to clarify the composition of the Z-line in insect's flight muscles.
Department of Evolutionary Biology, University of Siena, Italy.
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Riparbelli
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Callaini
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Dallai
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D004331:Drosophila melanogaster; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000737:chemistry* / Q000276:immunology*; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000737:chemistry* / Q000276:immunology; D009132:Muscles / Q000737:chemistry / Q000648:ultrastructure; D009210:Myofibrils / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007375:Interleukin-1; D009124:Muscle Proteins