Coinfection of the central nervous system by cytomegalovirus and herpes simplex virus type 1 or 2 in AIDS patients: autopsy study on 82 cases by immunohistochemistry and polymerase chain reaction.
We evaluated the frequency and histopathological features of concomitant infections of the central nervous system (CNS) with cytomegalovirus (CMV) and herpes simplex viruses type 1 or 2 (HSV1/2) in a large series of patients who had died from AIDS. Eighty-two autopsy cases with a histological diagnosis of CMV necrotizing encephalitis were examined retrospectively. CMV and HSV1/2 were detected by immunohistochemistry (IHC) with poly- and monoclonal antibodies and by nested polymerase chain reaction (PCR) for HSV 1 and 2 on DNA extracted from paraffin blocks. PCR for a beta-globin genomic sequence was performed in all IHC-positive cases to verify the integrity of extracted DNA. Concomitant CMV/HSV infections were demonstrated by IHC in 13 cases (16%); using monoclonal antibodies, HSV1 was found in 9 cases and HSV2 in 4 cases. In half of the cases, HSV1- or HSV2-positive cells represented more than 25% of immunopositive CMV cells. In all 13 cases, double immunochemical staining showed cells containing both CMV and HSV antigens. PCR for HSV1 and 2 was positive in only 7 of 13 cases (5 HSV1 and 2 HSV2). In the remaining 6 negative cases PCR for beta-globin was also repeatedly negative. HSV1 or 2 infection can be demonstrated by IHC in a significant proportion of AIDS cases with necrotizing CMV encephalitis. Nested PCR for HSV1 and 2 on DNA extracted from formalin-fixed and paraffin-embedded autopsy tissues was positive in only slighty above 50% of IHC-positive cases.
Pathology Unit, L. Sacco, Institute of Medical Sciences, University of Milan, Italy.
L
L
Vago
M
M
Nebuloni
E
E
Sala
P
P
Cinque
S
S
Bonetto
A
A
Isella
L
L
Ottoni
A
A
Crociati
G
G
Costanzi
D017088:AIDS-Related Opportunistic Infections / Q000235:genetics / Q000821:virology*; D002493:Central Nervous System Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification / Q000472:pathogenicity*; D004660:Encephalitis / Q000235:genetics / Q000276:immunology / Q000821:virology; D006566:Herpesviridae Infections / Q000235:genetics* / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification / Q000472:pathogenicity*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction