Calcium ionophore A 23187 induces apoptotic cell death in rat thymocytes.
Apoptotic cell death was induced in rat thymocytes on exposure to calcium ionophore A 23187 (100 micron(s)) for 24 h as observed from morphological changes and DNA fragmentation into oligonucleosomal ladder. The cell death was independent of de novo syntheses of protein. However, the involvement of c-Myc, c-Jun, poly ADPR polymerase and antioxidant enzymes CuZn SOD and catalase was observed.
Department of Biochemistry, A.I.I.M.S., Ansari Nagar, New Delhi, India.
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S
Azmi
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Dhawan
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Singh
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001549:Benzamides / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology
D001549:Benzamides; D004791:Enzyme Inhibitors; D007476:Ionophores; D011500:Protein Synthesis Inhibitors; D000001:Calcimycin; C025160:3-aminobenzamide; D003513:Cycloheximide; D015032:Zinc