The combined effects of plasma and hydrogel coating on adhesion of Staphylococcus epidermidis and Staphylococcus aureus to polyurethane catheters.
The adhesion of three Staphylococcus epidermidis and three S aureus clinical isolates, to uncoated and hydrogel-coated polyurethane catheters was tested, following pretreatment of catheters with human plasma. Plasma significantly decreased the adhesion of S. epidermidis strains to uncoated polyurethane catheters, but had no significant effect on the adhesion to hydrogel-coated catheters. The influence of plasma on adhesion of S. aureus strains to catheters was strain dependent. Plasma significantly increased the adhesion of one strain (SA6) to uncoated catheters. For two other strains (SA3 and SA14) plasma produced no clear effect on their adhesion to uncoated catheters; adhesion values for each strain showed either a small but significant increase or a replicate-dependent increase or decrease. However, plasma significantly increased the adhesion of all S. aureus strains to hydrogel-coated polyurethane catheters. Overall, with the exception of one batch culture of S. epidermidis strain SE3 tested, attachment to plasma-treated hydrogel coated catheters was statistically significantly lower, by up to 85%, than attachment to plasma-treated uncoated catheters for both S. epidermidis and S. aureus.
John
S F
SF
School of Biological Sciences, Manchester University, UK.
Derrick
M R
MR
Jacob
A E
AE
Handley
P S
PS
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D002404:Catheterization; D002405:Catheterization, Central Venous; D004866:Equipment Contamination; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010949:Plasma; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011140:Polyurethanes / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects* / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000166:cytology / Q000187:drug effects* / Q000302:isolation & purification
D011140:Polyurethanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols