Strong induction of activin expression after hippocampal lesion.
We studied the temporal and spatial mRNA expression pattern of activin/inhibin beta A, beta B and alpha subunits after unilateral kainic acid lesions of the hippocampal CA3 region. We found a strikingly increased expression of beta A mRNA in the ipsilateral hippocampus 6-24 h after injury. By contrast, the beta B and alpha mRNAs were expressed at equally low levels in normal and injured hippocampi, suggesting that the beta A transcripts give rise to activin A, but not to activin AB or inhibin. In situ hybridization demonstrated the presence of beta A mRNA in neurones near the site of lesion. Expression of all known types of activin receptors could be demonstrated in normal and injured hippocampi by RT-PCR. These findings suggest a role of activin in brain injury.
Physiologisches Institut der Universität, München, Germany.
Y P
YP
Tretter
B
B
Munz
G
G
Hübner
G
G
ten Bruggencate
S
S
Werner
C
C
Alzheimer
D028341:Activins; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D017403:In Situ Hybridization; D007265:Inhibins / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D028341:Activins; D007265:Inhibins; D007608:Kainic Acid