Amelioration by cAMP of cephaloridine-induced injury in the porcine kidney cell line LLC-PK1.
We investigated the effects of a phosphodiesterase inhibitor and dibutyryl cAMP (dBcAMP) on the cell injury induced by cephaloridine (CER) in an established renal epithelial cell LLC-PK1. CER increased the leakage of lactate dehydrogenase (LDH) from LLC-PK1 cells to the medium and the level of lipid peroxidation in the cells. 3-Isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, increased cAMP content in LLC-PK1 cells and ameliorated the increase in LDH leakage induced by CER, dBcAMP reduced the cell injury induced by CER. Our results suggest that a signalling pathway of cAMP protects against CER-induced renal cell injury, which is probably due to generation of oxygen radicals.
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
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Kawai
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Gemba
D058186:Acute Kidney Injury / Q000188:drug therapy; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002509:Cephaloridine / Q000009:adverse effects / Q000494:pharmacology*; D002511:Cephalosporins / Q000009:adverse effects / Q000494:pharmacology*; D004195:Disease Models, Animal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D018374:LLC-PK1 Cells / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D013552:Swine
D002511:Cephalosporins; D003994:Bucladesine; D007770:L-Lactate Dehydrogenase; D002509:Cephaloridine