Bacterial poly(A) polymerase: an enzyme that modulates RNA stability.
We have constructed a strain that overexpresses E coli poly(A) polymerase (PAP I). The recombinant protein was soluble, and a partially purified extract had high levels of poly(A) polymerising activity. An antiserum raised against the overexpressed PAP I has permitted two types of analysis: the identification of other E coli proteins that may interact with PAP I, and the search for PAP I-like proteins in other bacteria. Immunoprecipitation experiments suggest that PAP I is associated with a 48-kDa protein. This protein remains to be identified. Western blotting using the antiserum against E coli PAP I revealed related proteins in a variety of Gram-negative bacteria and in B subtilis. A comparison of the E coli protein with putative poly(A) polymerases recently identified in H influenza and B subtilis showed highly conserved sequences in the amino terminal and central portions of the proteins that may be important for enzyme activity.
Laboratoire de Microbiologie et Génétique Moléculaire, UPR 9007, CNRS, Toulouse, France.
L C
LC
Raynal
H M
HM
Krisch
A J
AJ
Carpousis
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D017124:Conserved Sequence; D004926:Escherichia coli / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D011233:Precipitin Tests; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012313:RNA; D011062:Polynucleotide Adenylyltransferase