Preimplantation embryo chromosome analysis by primed in situ labeling method.
OBJECTIVE
To present the use of primed in situ labeling method in preimplantation diagnosis.


METHODS
Double- and triple-primed in situ labeling were performed on 10 morphologically abnormal preimplantation embryos, using combinations of specific primers for chromosomes 9, 13, 16, 18, 21, X, and Y.


METHODS
Embryos were obtained from patients at the Montpellier University Hospital.


METHODS
Seven women undergoing IVF at the Montpellier University Hospital.


METHODS
Isolated interphase nuclei from poor quality preimplantation embryos were prepared for primed in situ labeling technique.


METHODS
Numerical abnormalities assessed by primed in situ labeling analysis.


RESULTS
Using directly fluorescent-labeled nucleotides, the labeling reaction for three chromosomes did not exceed 2.30 hours. Only three analyzed embryos appeared to be chromosomally normal. Mosaicism, aneupoidy, and haploidy were observed in the seven other embryos.


CONCLUSIONS
The primed in situ labeling method offers a simple and reliable screening tool for gender determination and aneuploidy detection. The use of this technique may contribute to significantly improve the procedure of preimplantation diagnosis.
Centre National de la Recherche Scientifique (CNRS), Montpellier, France.
F
F
Pellestor
A
A
Girardet
B
B
Andréo
G
G
Lefort
J P
JP
Charlieu
D000782:Aneuploidy; D001755:Blastocyst / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D047108:Embryonic Development; D005260:Female; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009030:Mosaicism; D011247:Pregnancy; D014960:X Chromosome; D014998:Y Chromosome
D017931:DNA Primers