Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas.
We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.
Department of Dermatovenereology, Erasmus University, University Hospital, Rotterdam, The Netherlands.
M F
MF
Baay
W G
WG
Quint
J
J
Koudstaal
H
H
Hollema
J M
JM
Duk
M P
MP
Burger
E
E
Stolz
P
P
Herbrink
D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D017931:DNA Primers; D004279:DNA, Viral