Mechanisms of triggered activity induction at the border zone of normal and abnormal cardiac tissue.
Ionic mechanisms that may be involved in inducing triggered activations at the border zone (BZTAs) of normal and abnormal Purkinje fiber segments were investigated. In a two-chamber bath, fibers were divided into a normal segment and segment treated with ethylenediaminetetraacetic acid to stimulate electrophysiologic alterations 24 hours after infarct. Interventions to normal segments included 1.8 mM lidocaine (n = 10), 3 x 10(-4) mM tetrodotoxin (n = 5), 10(-3) mM aconitine (n = 4), 3 mM cesium chloride (n = 7), 10(-2) mM verapamil (n = 4), and 6-8 mM (n = 7) of K+. Ethylenediaminetetraacetic acid (3.3 mM) prolonged action potentials and induced low diastolic potentials in the normal segment border zone. Tetrodotoxin, lidocaine, and high K+ levels suppressed BZTAs; cesium chloride and aconitine increased BZTAs; and verapamil did not reduce BZTAs. The finding that BZTAs were not abolished by verapamil suggests that abnormal automaticity is not a mechanism. Apparently, BZTAs depend on the Na+ inward current activated by depolarization of the membrane secondary to depolarization of adjacent cells.
Department of Medicine, University of Louisville, KY 40292, USA.
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C
Maldonado
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Li
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Wead
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Szabo
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Kupersmith
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008839:Microelectrodes; D009203:Myocardial Infarction / Q000503:physiopathology*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011690:Purkinje Fibers / Q000187:drug effects / Q000502:physiology*; D012756:Sheep; D009626:Terminology as Topic; D013997:Time Factors
D002317:Cardiovascular Agents; D004492:Edetic Acid