Expression of NF-Y nuclear factor in Schistosoma mansoni.
The A subunit of NF-Y nuclear factor from Schistosoma mansoni was expressed in E. coli fused to a histidine tag and purified by affinity chromatography using a Ni(2+)-Agarose matrix. Antibodies against the recombinant protein were prepared and used for Western blot and immunolocalization. The presence of SMNF-YA in all stages of the parasite life-cycle was determined by RT-PCR and Western blot analysis. The immunolocalization of SMNF-YA showed the presence of this factor in a parenchymal cell population of cercariae and adult worms and in embryos within eggs. The expression of SMNF-YA was demonstrated to decrease in maturating spermatozoites whereas an accumulation of this factor was observed in the nucleus from oocytes during their maturation processes.
Serra
E
E
Unité INSERM 167, Institut Pasteur Lille, France.
Liu
J L
JL
Capron
A
A
Zemzoumi
K
K
Dissous
C
C
D000818:Animals; D001700:Biomphalaria; D022762:CCAAT-Enhancer-Binding Proteins; D002454:Cell Differentiation; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D011994:Recombinant Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors