Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis.
Effective distinction was achieved among a wide range of lupin grain samples by either sodium dodecyl sulphate (SDS)-gel electrophoresis or capillary electrophoresis, based on grain-protein composition. Capillary electrophoresis was faster (< 1 h) and provided slightly greater distinction between the samples. On the other hand, SDS-gel electrophoresis could provide a greater through-put of samples in a 24 h period. Either technique could be used successfully to distinguish between lupin species and cultivars for taxonomic analysis or seed identification.
CSIRO Grain Quality Research Laboratory, Division of Plant Industry, North Ryde, Australia. naomi.pollard@pi.csiro.au
N J
NJ
Pollard
C W
CW
Wrigley
F
F
Bekes
A
A
Aumatell
F
F
MacRitchie
D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000235:genetics*; D005838:Genotype; D008832:Microchemistry; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal; D012680:Sensitivity and Specificity; D013045:Species Specificity
D010940:Plant Proteins