A coplanar PCB induces a selenium binding protein as a major cytosolic protein in rat liver.
We obtained evidence that a toxic coplanar polychlorinated biphenyl (PCB) induces a counterpart of murine 56kDa selenium binding protein in rat liver cytosol. A 54kDa protein in the liver cytosol was significantly induced by 3,3',4,4',5-pentachlorobiphenyl and proved to be a major cytosolic protein in the rat liver. The protein exhibited pI of 6.8 on two-dimensional gel electrophoresis. The amino acid sequence of peptide fragments from the protein digested in situ, was highly similar to a 56kDa selenium binding protein and similar to an acetaminophen binding protein in mice.
Faculty of Pharmaceutical Sciences, Kyushu University-62, Higashi-ku, Fukuoka, Japan.
Y
Y
Ishii
M
M
Hatsumura
T
T
Ishida
N
N
Ariyoshi
K
K
Oguri
D000595:Amino Acid Sequence; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis*; D003600:Cytosol / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D051137:Selenium-Binding Proteins; D013154:Spleen / Q000033:anatomy & histology / Q000187:drug effects; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects
D002352:Carrier Proteins; C000622413:Selenbp1 protein, rat; D051137:Selenium-Binding Proteins; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl