Simultaneous obtention of an intense G-banding and chromosome painting.
A method for simultaneously obtaining brilliant G-banding and chromosome painting is described. It is based on a modification of methods enabling the detection of incorporated BrdU by anti-BrdU antibodies conjugated to a fluorochrome. It induces a banding of both painted and non-painted chromosomes. The method was adapted for in situ hybridizations on human and non human (ZOO-FISH) chromosomes.
UMR 147, Cytogénétique Moléculaire et Oncologie CNRS-Institut Curie, Paris, France. frichard@curie.fr
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F
Richard
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B
Dutrillaux
D000818:Animals; D001973:Bromodeoxyuridine; D002428:Cebus; D002871:Chromosome Banding / Q000379:methods*; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000648:ultrastructure; D008677:Metaphase; D013045:Species Specificity
D001973:Bromodeoxyuridine