Thymine dimer-dependent incision on ultraviolet light damaged-DNA in cell-free extracts of Chlorella pyrenoidosa.
Cell-free extracts of the unicellular algae Chlorella pyrenoidosa induce a UV-dependent incision of both phi x 174 RFI DNA and a 27-mer double-stranded oligonucleotide containing a single thymine dimer, as analyzed by agarose and denaturing polyacrylamide gel electrophoresis, respectively. The incision reaction occurred only on the thymine dimer-containing strand of the 27-mer, and a major incision product of 17-mer was observed. Addition of ATP or ATP-regenerating system to algal extracts increased the effect of DNA incision.
Chang
C W
CW
Institute of Marine Biotechnology, National Taiwan Ocean University, Taiwan, Republic of China.
Ho
J C
JC
Hsu
T
T
D001483:Base Sequence; D002474:Cell-Free System; D002708:Chlorella / Q000737:chemistry*; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D008969:Molecular Sequence Data; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D011740:Pyrimidine Dimers; D004247:DNA; D013941:Thymine