The cDNA sequence encoding bovine SP-22, a new defence system against reactive oxygen species in mitochondria.
We have isolated cDNA clones coding SP-22, an antioxidant protein in mitochondria, from a bovine adrenal medulla cDNA library constructed with (lambda)gt11. The largest clone contained the entire coding sequence for mature SP-22. Since the isolated cDNA clones lacked 5'- and 3'-ends, we determined the sequences of both ends by the "Rapid Amplification of cDNA Ends (RACE)" tecnique. The deduced amino acid sequence of the mature protein region was the same as that determined by protein sequencing. Since SP-22 had a mitochondrial targetting signal, its mitochondrial localization was confirmed.
Radioisotope Research Institute, St. Marianna University School of Medicine, Kawasaki, Japan.
T
T
Hiroi
S
S
Watabe
K
K
Takimoto
N
N
Yago
Y
Y
Yamamoto
S Y
SY
Takahashi
D000313:Adrenal Medulla / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D018076:DNA, Complementary; D004272:DNA, Mitochondrial / Q000737:chemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D010544:Peroxidases; D054464:Peroxiredoxins; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D000975:Antioxidants; D017931:DNA Primers; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D010544:Peroxidases; D054464:Peroxiredoxins