Expression of NF-Y nuclear factor in Schistosoma mansoni.
The A subunit of NF-Y nuclear factor from Schistosoma mansoni was expressed in E. coli fused to a histidine tag and purified by affinity chromatography using a Ni(2+)-Agarose matrix. Antibodies against the recombinant protein were prepared and used for Western blot and immunolocalization. The presence of SMNF-YA in all stages of the parasite life-cycle was determined by RT-PCR and Western blot analysis. The immunolocalization of SMNF-YA showed the presence of this factor in a parenchymal cell population of cercariae and adult worms and in embryos within eggs. The expression of SMNF-YA was demonstrated to decrease in maturating spermatozoites whereas an accumulation of this factor was observed in the nucleus from oocytes during their maturation processes.
Unité INSERM 167, Institut Pasteur Lille, France.
E
E
Serra
J L
JL
Liu
A
A
Capron
K
K
Zemzoumi
C
C
Dissous
D000818:Animals; D001700:Biomphalaria; D022762:CCAAT-Enhancer-Binding Proteins; D002454:Cell Differentiation; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D011994:Recombinant Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors