Cloning and sequencing of the group 6 allergen of Dermatophagoides pteronyssinus.
BACKGROUND
The allergen Der p 6 from Dermatophagoides pteronyssinus has been described by substrate affinity as mite chymotrypsin.


OBJECTIVE
The aim of this paper was to describe a cDNA clone encoding the allergen.


METHODS
cDNA was cloned from a lambda library using oligonucleotides published for Der p 6.


RESULTS
The clone P6.1.1 had the N-terminal amino acid residues as reported for Der p 6, and at position 189 the Ser was conserved in chymotrypsin for substrate specificity as well as the catalytic triad of His57, Asp102 and Ser195 for serine proteases. The estimated M(r) was 24.9K and it was 37% identical to the trypsin allergen Der p 3.


CONCLUSIONS
cDNA encoding Der p 6 has been described.
TVW Telethon Institute for Child Health Research, Perth, Australia.
B J
BJ
Bennett
W R
WR
Thomas
D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D002918:Chymotrypsin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008925:Mites / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D006023:Glycoproteins; D002918:Chymotrypsin