Neurogranin is locally concentrated in rat cortical and hippocampal neurons.
The rat protein kinase C substrate neurogranin has a granular distribution in cortical and hippocampal neurons. We demonstrate that in these cells, granular labelling corresponds to a local concentration of neurogranin-immunoreactivity at both the membranes of mitochondria and trans-Golgi vesicles and soma-proximal dendritic shaft and spinal head structures. Our findings suggest that the function of neurogranin could be affected by protein assembly at these cellular sites.
Neuner-Jehle
M
M
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, C.N.R.S. Bât. CERVI, Hôpital de la Pitié Salpêtrière, Paris, France.
Denizot
J P
JP
Mallet
J
J
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002148:Calmodulin-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002540:Cerebral Cortex / Q000166:cytology*; D003712:Dendrites / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000737:chemistry; D006624:Hippocampus / Q000166:cytology*; D008297:Male; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D051579:Neurogranin; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism; D017966:Pyramidal Cells / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; C496651:Nrgn protein, rat; D051579:Neurogranin; D011493:Protein Kinase C