Glycoprotein E of bovine herpesvirus type 1 is involved in virus transmission by direct cell-to-cell spread.
In order to identify the role of the bovine herpesvirus type 1 (BHV-1) glycoprotein E (gE) in the viral infection cycle, we have constructed a BHV-1 gE deletion mutant strain (BHV-1 gE-). This strain was assayed in vitro by comparing its growth kinetics with the wild type strain used as a host of the deletion. Our results indicate that those conditions which prevent the infection by direct adsorption to the cells (presence of a semi-solid medium or presence of neutralizing antibodies in the medium) selectively inhibit the growth of the gE- strain, suggesting that gE plays a central role in the BHV-1 spread by direct cell-to-cell transmission, a major mechanism of the BHV-1 in vivo virulence.
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Spain.
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X
Rebordosa
J
J
Piñol
J A
JA
Pérez-Pons
J
J
Lloberas
J
J
Naval
X
X
Serra-Hartmann
E
E
Espuña
E
E
Querol
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D018274:Electroporation; D016679:Genome, Viral; D001911:Herpesvirus 2, Bovine / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D007668:Kidney; D007700:Kinetics; D016296:Mutagenesis; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D017384:Sequence Deletion; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010948:Viral Plaque Assay; D014764:Viral Proteins; D014779:Virus Replication
D004279:DNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins