Insulin treatment can abolish changes in glucose and glutamine metabolism of lymphocytes and macrophages caused by the implantation of the Walker 256 tumour.
Activation of lymphocytes and macrophages by the implantation of tumour cells (10(7) cells per rat) into the left flank of rats increased the conversion of glucose to lactate and of glutamine to glutamate and aspartate and the decarboxylation of [U-14C]-glucose and [U-14C]-glutamine in incubated cells. In addition, the amount of GLUT1 was increased in macrophages. The effect of insulin treatment on glucose and glutamine metabolism of lymphocytes and macrophages activated by Walker 256 tumour implantation was also examined. For this purpose, insulin was injected subcutaneously (4 U/100 g b.w. daily) after the fourth day of tumour implantation and the rats were killed 10 days afterwards. Insulin treatment fully reverted the changes due to tumour implantation in the metabolism of glucose and glutamine in lymphocytes and of glucose in macrophages.
Departamento de Fisiologia, Universidade Federal do Paraná, Curitiba-Pr, Brasil.
L C
LC
Fernandes
C A
CA
Mattozo
U F
UF
Machado
L F
LF
Rosa
R
R
Curi
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002279:Carcinoma 256, Walker / Q000097:blood / Q000473:pathology; D051272:Glucose Transporter Type 1; D018698:Glutamic Acid / Q000097:blood; D005973:Glutamine / Q000097:blood*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000627:therapeutic use*; D019344:Lactic Acid / Q000097:blood; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000097:blood; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D051272:Glucose Transporter Type 1; D007004:Hypoglycemic Agents; D007328:Insulin; D009004:Monosaccharide Transport Proteins; C495679:Slc2a1 protein, rat; D005973:Glutamine; D019344:Lactic Acid; D018698:Glutamic Acid