Aspergillus nidulans stcP encodes an O-methyltransferase that is required for sterigmatocystin biosynthesis.
The Aspergillus nidulans stcP gene was previously identified as a transcribed region associated with a cluster of genes proposed to be involved in sterigmatocystin biosynthesis (D. W. Brown, J.-H. Yu, H. S. Kelkar, M. Fernandes, T. C. Nesbitt, N. P. Keller, T. H. Adams, and T. J. Leonard, Proc. Natl. Acad. Sci. USA 93:1418-1422, 1996). stcP was predicted to encode a methyltransferase responsible for conversion of demethylsterig-matocystin to sterigmatocystin. Here we demonstrate that disruption of stcP in A. nidulans results in strains that accumulate demethylsterigmatocystin.
Department of Plant Pathology, Texas A&M University, College Station 77843, USA.
H S
HS
Kelkar
N P
NP
Keller
T H
TH
Adams
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013241:Sterigmatocystin / Q000096:biosynthesis*
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D013241:Sterigmatocystin; D008780:Methyltransferases; C096006:omtA protein, Aspergillus