Automated multiple peptide synthesis: improvements in obtaining quality peptides.
Production of multiple overlapping peptides is a key step in the identification of T-cell epitopes. A large number of peptides can be produced by using ABIMED's automated multiple peptide synthesizer. We report here considerable improvement in the software and chemistry of peptide synthesis by introducing a resin mixing step during coupling, when using this synthesizer. A comparison of two solvent systems for synthesis was performed. Six test peptides were synthesized by standard and modified methods. The purity of peptides, assessed by HPLC and mass spectrometry, showed a substantial improvement when automated resin mixing and mixed solvent system were used. These improvements enable us to produce 48 peptides within a week each of sufficient purity to be used for rapid screening of T-cell epitopes.
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Anergen Inc., Redwood City, California, USA.
Pham
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Deshpande
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D001327:Autoimmune Diseases / Q000378:metabolism*; D001331:Automation; D001671:Biochemistry / Q000295:instrumentation*; D004126:Dimethylformamide; D018984:Epitopes, T-Lymphocyte / Q000032:analysis*; D008752:Methylene Chloride; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000592:standards; D012116:Resins, Plant
D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D012116:Resins, Plant; D008752:Methylene Chloride; D004126:Dimethylformamide