The reactivity of taurine with hypochlorous acid and its application for eye drops.
The ability of taurine to protect biomembranes attacked by HOCl was examined using canine erythrocytes which had been pre-treated with HOCl. Of the amino acids tested, taurine was the most effective in inhibiting attack by HOCl, followed by glycine, alpha-alanine and beta-alanine. During the incubation of HOCl-treated erythrocytes with taurine, an appreciable amount of monochlorotaurine (TauNHCl) was detected in the supernatant. This suggests that taurine may remove the oxidized chlorine from HOCl-treated erythrocytes, resulting in the production of TauNHCl. The effect of taurine on the removal of Cl+ moiety was examined using Sepharose gel with amino groups. Taurine removed Cl+ from HOCl-treated Sepharose gel. The yield of TauNHCl depended on the concentration of taurine. The effectiveness of taurine in preventing ocular surface damage caused by HOCl was investigated in albino rabbits. The activity of lactate dehydrogenase (LDH) released from ocular tissues into tears during eye irritation was used as an index of ocular surface damage. Taurine effectively protected ocular surface tissues from damage induced by HOCl, and arrested the progression of tissue damage that had already been initiated by HOCl. These finding suggest that taurine may be clinically useful in the treatment of ocular surface damage caused by oxidants such as HOCl.
Research Center, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
I
I
Koyama
K
K
Nakamori
T
T
Nagahama
M
M
Ogasawara
M
M
Nemoto
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002713:Chlorine / Q000097:blood; D003228:Conjunctiva / Q000502:physiology; D003315:Cornea / Q000502:physiology; D004285:Dogs; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005998:Glycine / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006997:Hypochlorous Acid / Q000737:chemistry* / Q000633:toxicity*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase; D008297:Male; D009883:Ophthalmic Solutions; D011817:Rabbits; D013654:Taurine / Q000737:chemistry* / Q000494:pharmacology*; D013666:Tears / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015091:beta-Alanine / Q000494:pharmacology
D009883:Ophthalmic Solutions; D015091:beta-Alanine; D013654:Taurine; D002713:Chlorine; D006997:Hypochlorous Acid; D007770:L-Lactate Dehydrogenase; D000409:Alanine; D005998:Glycine