Prevention of bacterial adherence to implant surfaces with a crosslinked albumin coating in vitro.
Titanium surfaces were coated with bovine serum albumin using carbodiimide, a crosslinking agent. The durability of the coated surfaces and the inhibitory effect of the albumin coating on bacterial adherence were tested in vitro for 20 consecutive days at 37 degrees C in phosphate buffered saline, with intermittent agitation. The results showed that only 10% of the coated bovine serum albumin decayed off the surface during the 20-day incubation period. The inhibition rate of the albumin coating on bacterial adherence remained high (greater than 8.5%) throughout the experiment. The results suggested potential use of this crosslinked albumin coating to reduce bacterial adherence and thus the subsequent possibility of prosthetic or implant infection in vivo.
Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston 29425, USA.
Y H
YH
An
G W
GW
Stuart
S J
SJ
McDowell
S E
SE
McDaniel
Q
Q
Kang
R J
RJ
Friedman
D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008422:Materials Testing; D008828:Microbiological Techniques; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology*; D014025:Titanium
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D012709:Serum Albumin; D014025:Titanium