Tissue-specific alternative splicing of mouse brain type ryanodine receptor/calcium release channel mRNA.
We detected alternative splicing of the mouse brain type ryanodine receptor (RyR3) mRNA. The splicing variant was located in the transmembrane segment. The non-splicing type (RyR3-II) included a stretch of 341 bp, and that of the 13th codon was stop codon TAA. Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows that RyR3-II mRNA was expressed in various peripheral tissues and brain at all developmental stages. However, interestingly, the splicing type (RyR3-I) mRNA was detected only in the cerebrum. These findings suggest that the splicing variants RyR3-I and RyR3-II may generate functional differences of RyR3 in a tissue-specific manner.
Department of Neuropsychiatry, Kagawa Medical University, Kita-gun, Japan.
R
R
Miyatake
A
A
Furukawa
M
M
Matsushita
K
K
Iwahashi
K
K
Nakamura
Y
Y
Ichikawa
H
H
Suwaki
D000375:Aging / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D015220:Calcium Channels / Q000096:biosynthesis* / Q000737:chemistry; D003001:Cloning, Molecular; D003062:Codon; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000737:chemistry; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D019837:Ryanodine Receptor Calcium Release Channel
D015220:Calcium Channels; D003062:Codon; D009124:Muscle Proteins; D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel