Phospholipid metabolism in Alzheimer's disease and in a human cholinergic cell.
There is evidence available suggesting that membrane alterations occur in Alzheimer's disease including the metabolism of membrane phospholipids. We have quantitated in vitro the phospholipase D activity of homogenates from Alzheimer's disease brain tissue. There was a significant increase of this enzyme activity as compared to controls. Amyloid beta protein is the predominant protein of the characteristic senile plaques found in Alzheimer's disease. Treatment of LA-N-2 cells, a human cholinergic neuroblastoma clone, with amyloid beta protein results in an activation of phospholipases A, C and D.
Kanfer
J N
JN
Department of Biochemistry and Molecular Biology, University of Manitoba, Faculty of Medicine, Canada.
Singh
I N
IN
Pettegrew
J W
JW
McCartney
D G
DG
Sorrentino
G
G
D000544:Alzheimer Disease / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D010743:Phospholipids / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*
D010743:Phospholipids; D011950:Receptors, Cholinergic