Physiological effectors of hyperglycemic neurohormone biosynthesis in an insect.
Biosynthesis of hypertrehalosemic hormone (HTH) by isolated corpora cardiaca (CC) from Blaberus discoidalis cockroaches was measured for the 3-week period following adult ecdysis using an in vitro bioassay based on the incorporation of [3H]tryptophan. Synthetic rates for both males and females were highest following the molt and declined with age. Rates of HTH secretion and levels of stored HTH followed a similar pattern. We found no neural or endocrine brain effects or photophase influence on HTH synthesis. Synthesis was unaffected by treatment with 20-hydroxyecdysone or the juvenile hormone analog methoprene. In starved insects, HTH synthesis doubled and secretion increased 66% relative to fed controls.
Department of Entomology, Texas A&M University, Texas Agricultural Experiment Station, College Station 77843, USA.
S M
SM
Sowa
K H
KH
Lu
J H
JH
Park
L L
LL
Keeley
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003058:Cockroaches / Q000254:growth & development / Q000502:physiology*; D005260:Female; D007301:Insect Hormones / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D009479:Neuropeptides / Q000096:biosynthesis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012727:Sex Characteristics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014364:Tryptophan / Q000378:metabolism
D007301:Insect Hormones; D009479:Neuropeptides; C084112:hypertrehalosemic hormone; D014364:Tryptophan