3'-5' L-capped-antisense oligodeoxynucleotides targeted at the SV40 T-antigen gene: pharmacological and biological properties.
This paper reports on some pharmacological and biological properties of 22-mer antisense oligodeoxynucleotides which contain an L-deoxyribonucleoside at each terminus. Compared with natural compounds, of which they retain the DNA hybridizing ability and the cell uptake mechanism, the L-22-mers exhibited an increased resistance to phosphodiesterase degradation, an apparent higher intracellular concentration and a longer intracellular half life. Antiviral activity was not prominent.
Institute of Microbiology, University of Padova, Italy.
E
E
Pettenazzo
B
B
Maschera
F
F
Bevilacqua
M L
ML
Capobianco
G
G
Palù
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006207:Half-Life; D006801:Humans; D009691:Nucleic Acid Denaturation; D016376:Oligonucleotides, Antisense / Q000493:pharmacokinetics / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013539:Simian virus 40 / Q000187:drug effects* / Q000235:genetics; D013601:T-Lymphocytes / Q000821:virology
D000952:Antigens, Polyomavirus Transforming; D016376:Oligonucleotides, Antisense; D010727:Phosphoric Diester Hydrolases