[Application of decoy strategy].
Recently, transfection of cis-element double stranded (ds) oligodeoxynucleotides (ODN) (= decoy) as a new powerful tool in a new class of anti-gene strategy for gene therapy and the study of transcriptional regulation has been reported. Transfection of ds ODN corresponding to cis sequence will result in the attenuation of authentic cis-trans interaction, leading to the removal of trans-factors from the endogenous cis-element with subsequent modulation of gene expression. Recent studies provide evidence of on in vivo application of this novel molecular approach as a therapeutic strategy against cardiovascular disease, and tool for the study of gene regulation in vivo.
Department of Geriatric Medicine, Osaka University Medical School.
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Morishita
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Ogihara
D001483:Base Sequence; D003327:Coronary Disease / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000627:therapeutic use*; D014162:Transfection
D009841:Oligonucleotides