Fingerprinting of glycans as their 2-aminoacridone derivatives by capillary electrophoresis and laser-induced fluorescence.
Capillary electrophoresis and laser-induced fluorescence detection have been used to fingerprint the 2-aminoacridone derivatives of complex glycans released from bovine fetuin and human IgG monoclonal antibodies. The utility of this method in distinguishing between N- and O-linked oligosaccharides and in determining the presence of sialic acid residues in glycan mixtures at an early stage of analysis has been demonstrated.
SmithKline Beecham Pharmaceuticals, Welwyn, Herts, UK.
G B
GB
Harland
G
G
Okafo
P
P
Matejtschuk
I C
IC
Sellick
G E
GE
Chapman
P
P
Camilleri
D000609:Aminoacridines / Q000032:analysis*; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007834:Lasers; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*; D000509:alpha-Fetoproteins / Q000032:analysis*
D000609:Aminoacridines; D007074:Immunoglobulin G; D011134:Polysaccharides; D000509:alpha-Fetoproteins; C072515:2-aminoacridone