Expression of mucin antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx.
Recent studies have identified that mucin antigens and Lewis X (Lex)-related antigens behave like oncodevelopmental tumor-associated antigens in several human adenocarcinomas. However, the expression of these antigens in pharyngeal and laryngeal squamous cell carcinomas (SCC) and in the precursor lesion is not fully elucidated yet. In the present study, the expression of mucin core protein antigens associated with the MUC1 gene product (DF3 antigen, mammary-type apomucin) and the MUC2 gene product (intestinal-MRP antigen, intestinal-type apomucin) mucin carbohydrate antigens that are associated with the earliest steps in mucin glycosylation (Tn, sialyl-Tn and T), and Lex-related antigens (Lex, Ley and sialyl Lex-i) in biopsy or resected specimens from 26 normal squamous epithelia (NSE), 49 dysplastic squamous epithelia (DSE) and 51 SCC were examined. The DF3 antigen was not expressed in NSE (0%), whereas it was expressed in 20 DSE (41%) and in 31 SCC (61%). The intestinal-MRP antigen showed no expression in NSE, DSE or SCC. The Tn antigen showed no expression in NSE, but showed low expression rates in DSE (14%) and in SCC (16%). The sialyl-Tn and T antigens were expressed in NSE, as well as in DSE and SCC. The T antigen expression increased with progression from NSE to DSE to SCC, while the sialyl-Tn antigen did not show such a tendency. Any of the three Lex-related antigens showed no characteristic expression in DSE and SCC. In the eight antigens examined, only DF3 antigen was an effective marker for DSE and SCC in the pharyngeal and laryngeal region. Cytoplasmic expression of DF3 and sialyl-Tn antigens were more frequently seen in SCC than in DSE, and might be useful to differentiate SCC from DSE.
Second Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
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T
Itoh
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Yonezawa
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M
Nomoto
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K
Ueno
Y S
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Kim
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E
Sato
D000951:Antigens, Neoplasm / Q000032:analysis*; D000957:Antigens, Viral, Tumor / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D005752:Gastric Mucins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000276:immunology*; D007983:Lewis Blood Group Antigens; D009077:Mucins / Q000276:immunology*; D009844:Oligosaccharides / Q000032:analysis*; D010610:Pharyngeal Neoplasms / Q000276:immunology*; D011230:Precancerous Conditions / Q000276:immunology*; D000080506:Sialyl Lewis X Antigen
D000951:Antigens, Neoplasm; D000957:Antigens, Viral, Tumor; D014408:Biomarkers, Tumor; D005752:Gastric Mucins; D007983:Lewis Blood Group Antigens; D009077:Mucins; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C053366:apomucin