Catalytic role of the alpha-carboxylate of the Glu residue of glutathione in glutathione S-transferases.
The recently proposed catalytic role of the alpha-carboxylate of the Glu residue of glutathione in glutathione S-transferases (Widersten et al, Biochemistry 35, 7731-7742 (1996)) was examined. Based on structural considerations, it is clear that conformational changes in both glutathione and glutathione S-transferase are required. Recent kinetic studies by Ricci and coworkers (Ricci et al, J. Biol. Chem. 271, 16187-16192 (1996) and Caccuri et al, J. Biol. Chem. 271, 16193-16198 (1996)) may provide the missing evidence for these conformational changes. Possible ways to test this hypothesis are discussed.
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. vg857@dewar.pnl.gov
Y J
YJ
Zheng
R L
RL
Ornstein
D002264:Carboxylic Acids / Q000737:chemistry*; D002384:Catalysis; D018698:Glutamic Acid / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D002264:Carboxylic Acids; D018698:Glutamic Acid; D005982:Glutathione Transferase; D005978:Glutathione