Morphometric and immunohistochemical studies on atrophic changes in lympho-hematopoietic organs of rats treated with piperonyl butoxide or subjected to dietary restriction.
Changes observed in lympho-hematopoietic organs in rats given piperonyl butoxide may be attributable either to direct toxic effects or to undernutrition. Male F344 rats were therefore fed diet containing 2.5% piperonyl butoxide or subjected to a 64% restriction of food intake for 2 weeks. Marked inhibition of body weight gain, decreased white blood cell count, depletion of T/B lymphocytes in lymphoid tissues, hypoplasia of the bone marrow, and decreased proliferating cell nuclear antigen (PCNA) labeling indices in these tissues were seen in both dietary restriction and 2.5% piperonyl butoxide groups. The depletion of T lymphocytes in the thymus and spleen was stronger in the 2.5% piperonyl butoxide group, as indicated by PCNA labeling indices and image analysis of T lymphocyte areas of the spleen, however, the toxicological profile observed for the chemically treated group was essentially the same as for animals on the restricted diet. These results suggest that the lympho-hematopoietic findings in rats receiving 2.5% piperonyl butoxide are probably due to undernutrition resulting from a reduced food intake.
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
K
K
Mitsumori
K
K
Takegawa
T
T
Shimo
H
H
Onodera
K
K
Yasuhara
M
M
Takahashi
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D004435:Eating; D004906:Erythrocyte Count / Q000187:drug effects; D006410:Hematopoiesis; D007150:Immunohistochemistry; D018655:Lymphocyte Count; D008297:Male; D009748:Nutrition Disorders / Q000473:pathology*; D010574:Pesticide Synergists / Q000008:administration & dosage / Q000633:toxicity*; D010882:Piperonyl Butoxide / Q000008:administration & dosage / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
D010574:Pesticide Synergists; D018809:Proliferating Cell Nuclear Antigen; D010882:Piperonyl Butoxide