Time-resolved biophysical methods in the study of protein folding.
Many of the biophysical techniques developed to characterize native proteins at equilibrium have now been adapted to the structural and thermodynamic characterization of transient intermediate populations during protein folding. Recent advances in these techniques, the use of novel methods of initiating refolding, and a convergence of theoretical and experimental approaches are leading to a detailed understanding of many aspects of the folding process.
Plaxco
K W
KW
New Chemistry Laboratory, University of Oxford, UK. kwp@bioch.ox.ac.uk
Dobson
C M
CM
D000818:Animals; D006801:Humans; D008722:Methods; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*
D011506:Proteins