Distribution of vimentin in abnormal human spermatozoa.
Vimentin was immunocytochemically detected using a monoclonal anti-vimentin antibody and indirect immunogold electron microscopy. In abnormal human spermatozoa, vimentin was visualized at the level of whole acrosome, post-acrosomal region, neck and initial segment of the mid piece. The distribution of vimentin immunoreactivity was related to a spectrum of structural defects such as large cytoplasmic droplets, binucleated spermatozoa and mitochondrial disassembly in the mid piece.
Department of Biology, Medical University, Sofia, Bulgaria.
T T
TT
Marinova
M D
MD
Markova
R K
RK
Stanislavov
D000328:Adult; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D013094:Spermatozoa / Q000002:abnormalities / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
D014746:Vimentin