Co-amplification to tail-to-tail copies of MuRVY and IAPE retroviral genomes on the Mus musculus Y chromosome.
We have isolated a clone from a C57BL/6 genomic library that contains both part of the Y Chromosome-specific 8.7 kbp MuRVY genome (Hutchinson and Eicher, J. Virol. 63, 4043, 1989) and a full-length 8.3 kbp Intracisternal A Particle genome (IAPE-Y), in a tail-to-tail organization. Although IAPs are encoded by a disperse multigene family (approximately 1000 copies per haploid genome), we present evidence that a significant proportion of the IAP-related sequences are present on the Y Chromosome (Chr) and that a >25 kbp genomic sequence, which contains the two proviral genomes, has been amplified on the Y Chr. Two discrete amplified families of MuRVY retroviral genomes distinguishable by a polymorphic restriction site were detected, suggestive that amplification occurred in incremental stages. The presence of MuRVY-related DNA sequences, but absence of IAPE-Y-related DNA sequences in Mus spretus suggests that the IAPE-Y retrotransposition event occurred after the evolutionary divergence of the lineages leading to Mus musculus and Mus spretus, and that the amplification of MuRVY occurred independently in the two lineages.
Fennelly
J
J
MRC Radiobiology Unit, Chilton, Didcot, Oxon, UK.
Harper
K
K
Laval
S
S
Wright
E
E
Plumb
M
M
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D019143:Evolution, Molecular; D018628:Gene Dosage; D005814:Genes, Viral / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*