Possible cytoprotective mechanism in rats of D-002, an anti-ulcerogenic product isolated from beeswax.
D-002 is an anti-ulcerogenic product, isolated from beeswax, which consists of a well-defined mixture of higher primary aliphatic alcohols. It is highly effective against ethanol-induced ulcers. This study was designed to determine if D-002 shows cytoprotective properties on gastric mucosa in ethanol-induced ulcers. The involvement of endogenous prostaglandins in the protective effect of D-002 was also investigated. When a subulcerogenic dose of indomethacin (10 mg kg-1) was injected simultaneously with oral administration of ethanol, oral pre-treatment with D-002 (5-100 mg kg-1) partially inhibited the gastric protection. D-002 (5 and 25 mg kg-1) administered to normal rats significantly increased the soluble mucus content and also prevented its reduction in rats with ethanol-induced ulcers. In addition, D-002 administered at 5 and 25 mg kg-1 prevented the increase of vascular permeability induced by ethanol (60%) and reduced the concentration of thromboxane B2 (TXB2) in gastric mucosa of rats with ethanol-induced ulcers. These results support the hypothesis that the anti-ulcerogenic properties of D-002 could be related to a cytoprotective mechanism.
Carbajal
D
D
Department of Pharmacology, Center of Natural Products, National Center for Scientific Research, Habana, Cuba.
Molina
V
V
Valdés
S
S
Arruzazabala
L
L
Rodeiro
I
I
Más
R
R
Magraner
J
J
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D001516:Bees / Q000737:chemistry*; D002199:Capillary Permeability / Q000187:drug effects; D000431:Ethanol; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000627:therapeutic use*; D005260:Female; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D009093:Mucus / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013929:Thromboxane B2 / Q000378:metabolism; D014456:Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D014885:Waxes / Q000737:chemistry
D000897:Anti-Ulcer Agents; C104511:D 002; D005233:Fatty Alcohols; D011453:Prostaglandins; D014885:Waxes; C038228:beeswax; D000431:Ethanol; D013929:Thromboxane B2