Detection of Salmonella DNA in chicken embryos and environmental samples by polymerase chain reaction.
By polymerase chain reaction (PCR) using a pair of primers specific for Salmonella phoE gene a 365-bp specific gene fragment could be amplified from yolk of infertile eggs and dead-in-shell chicken embryos, and from environmental samples. Out of 45 dead-in-shell embryo samples, 20 (44.4%) were found positive for Salmonella DNA by PCR compared to 11 (24.4%) by bacteria isolation. Salmonella DNA could also be detected from infertile eggs, chicken faeces, floor litter and chick fluff, which incidence was higher than that by bacteria isolation.
Tuchili
L M
LM
School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Kodama
H
H
Sharma
R N
RN
Takatori
I
I
Pandey
G S
GS
Kabilika
S
S
Mukamoto
M
M
Tsuji
S
S
Baba
T
T
D000818:Animals; D002642:Chick Embryo / Q000737:chemistry*; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005243:Feces / Q000737:chemistry; D015994:Incidence; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology; D012475:Salmonella / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000453:epidemiology
D004269:DNA, Bacterial