Changes of intermediate filaments in cultured human glioma cells with various growth factors and cytokines using high voltage immunoelectron microscopy.
Morphological and immunocytological changes of intermediate filaments of cultured human malignant glioma cells were studied by adding various growth factors or cytokines using stereoscopic high voltage electron microscopy operated at 1,000 kV. The gold-colloid immuno-cytochemical method was used to stain GFAP and vimentin. Growth rate of tumor cells increased when EGF, TGF-alpha, and PDGF administered and decreased when FGF, TNF, and CLN-IgG administered. Morphological changes of cells were not remarkable when EGF, PDGF, IL-1, and FGF were administered. The cytoplalsmic organellaes were damaged after administrating TNF and CLN-IgG to cells.
Department of Neurosurgery, Hyogo Medical Center for Adults.
K
K
Taomoto
A
A
Ijichi
T
T
Arii
K
K
Hama
D001932:Brain Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D002455:Cell Division / Q000502:physiology; D016207:Cytokines / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005910:Glioma / Q000378:metabolism / Q000648:ultrastructure*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000648:ultrastructure; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D016207:Cytokines; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D016211:Transforming Growth Factor alpha; D014746:Vimentin; D004815:Epidermal Growth Factor