Detection of genes regulating beta-lactamase production in Enterococcus faecalis and Staphylococcus aureus.
Four beta-lactamase-producing clinical isolates (WH245, WH257, CH570, and DEL) of Enterococcus faecalis were examined for the presence of the staphylococcal beta-lactamase regulatory genes (blaR1 and blaI) by PCR using six primer pairs. All isolates produced small amounts of beta-lactamase constitutively. In WH245, CH570, and DEL, the corresponding regions of the regulatory genes have lost sequences of various lengths. However, the regulatory genes in WH257 appeared to be the same as those in staphylococcal plasmid pI258. The beta-lactamase genes could be transferred to enterococcal and staphylococcal recipients from WH257 and DEL by conjugation or transformation with selection for gentamicin resistance. After transformation, the expression of beta-lactamase from DEL was still constitutive, whereas the gene from WH257 showed inducible expression in Staphylococcus aureus. The gene coding for inducible beta-lactamase production from pI258 showed constitutive expression in E. faecalis. These findings suggest that constitutive beta-lactamase production in E. faecalis is due not only to the absence of functional regulatory genes but to some other factor(s) as well.
Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan.
R
R
Okamoto
T
T
Okubo
M
M
Inoue
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases