Remnant-like particles (RLP) from NIDDM patients with apolipoprotein E3/3 phenotype stimulate cholesteryl ester synthesis in human monocyte-derived macrophages.
We tested the ability of remnant-like particles (RLP) from NIDDM patients to stimulate cholesteryl ester synthesis in human monocyte-derived macrophages. Six NIDDM patients were studied together with 7 non-diabetic subjects. All had apolipoprotein (apo) E3/3 phenotype. RLP were isolated using an immunoaffinity gel mixture of anti apo B-100 and anti apo A-1 monoclonal antibodies coupled to Sepharose 4B. Plasma levels of triglyceride, total cholesterol (chol) and high density lipoprotein-chol were not statistically different, but plasma levels of RLP-chol were significantly (p < 0.05) higher in NIDDM patients (10.5 +/- 2.2 mg/dl) than in non-diabetic controls (5.0 +/- 1.7 mg/dl). The effects of RLP from NIDDM patients on macrophage cholesteryl ester synthesis were estimated. 14C-oleate incorporation into cholesteryl esters in macrophages was significantly (p < 0.01) higher in NIDDM patients with apo E3/3 (0.326 +/- 0.037 nmole/mg cell protein) than in non-diabetic controls with apo E3/3 (0.181 +/- 0.011 nmole/ mg cell protein). It is suggested that RLP from NIDDM play a role in the accumulation of cholesteryl esters and are one of the risk factors for the acceleration of atherosclerosis in NIDDM.
Saito
M
M
Second Dept of Internal Medicine, Asahikawa Medical College, Japan.
Eto
M
M
Okada
M
M
Iwashima
Y
Y
Makino
I
I
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I / Q000276:immunology; D053299:Apolipoprotein B-100; D053318:Apolipoprotein E3; D001055:Apolipoproteins B / Q000276:immunology; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000096:biosynthesis*; D008076:Cholesterol, HDL / Q000097:blood; D002846:Chromatography, Affinity; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009000:Monocytes; D019301:Oleic Acid / Q000378:metabolism; D010641:Phenotype; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100; D053318:Apolipoprotein E3; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002788:Cholesterol Esters; D008076:Cholesterol, HDL; D014280:Triglycerides; D019301:Oleic Acid; D002784:Cholesterol