Molecular analysis of the rpoD gene of Enterococcus faecalis.
The complete nucleotide sequence of the rpoD gene of Enterococcus faecalis ATCC19433 has been determined. This gene encodes a putative 368 amino acids (aa) polypeptide of a predicted Mr of 41842 named sigma 42. Upstream of the rpoD gene and beginning from the end of the cloned chromosomal fragment is an open reading frame encoding a putative 264-aa polypeptide. It is reminiscent of the C-terminal domain of known DNA primase from Gram-positive bacteria indicating that the E. faecalis rpoD gene is included in a macromolecular synthesis (MMS) operon, as it was described for Escherichia coli, Bacillus subtilis and Lactococcus lactis rpoD gene.
Laboratoire de Microbiologie de l'Environnement, IRBA, Université de Caen, France. frere@clenche.msh.unicaen.fr
J
J
Frère
X
X
Gansel
A
A
Benachour
Y
Y
Auffray
D000595:Amino Acid Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics*; D013728:Terminator Regions, Genetic
D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases