Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection.
A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of carbohydrate derivatized and determined by CE/LIF was 10 pmol contained in 100 microL of the final reaction mixture. The CE/LIF method provided a linear response for the carbohydrates tested (over 2-3 orders of magnitude), with a limit of detection of 100 amol. Separation efficiencies over 250,000 theoretical plates were obtained. The procedure was used to analyze a tear fluid sample to demonstrate the applicability of the method for the glucose determination in small volumes of biological samples.
Perez
S A
SA
Department of Chemistry, State University of New York at Buffalo 14260-3000,
Colón
L A
LA
D003619:Dansyl Compounds / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D007834:Lasers; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis*; D013666:Tears / Q000737:chemistry
D003619:Dansyl Compounds; D004187:Disaccharides; D005456:Fluorescent Dyes; D006834:Hydrazines; D009005:Monosaccharides; C027913:dansyl hydrazine