Sequence analysis and genetic mapping of porcine chromosome 11 centromeric S0048 marker.
We report the existence of a new family of swine centromeric satellite DNA composed of a 51-bp repeat unit, most specifically found on pig chromosome 11 centromere and with less specificity at the centromeric region of other meta- and submetacentric chromosomes. This satellite DNA family, which has no homologies with the Mc1 and Ac2 families published previously, was named Mc2. We designed a specific primer set for PCR amplification of this centromeric satellite DNA. Specificity of amplification was checked by using a porcine somatic cell hybrid panel and by FISH. Furthermore, the development of a PCR-RFLP marker of Mc2 repetition allowed its genetic mapping on the PiGMaP reference families panel. The centromere of chromosome 11 was thus integrated to the genetic map previously published.
Laboratoire de Génétique Cellulaire, INRA BP27, Castanet-Tolosan France. riquet@toulouse.inra.fr
J
J
Riquet
P
P
Mulsant
M
M
Yerle
M S
MS
Cristobal-Gaudy
P
P
Le Tissier
D
D
Milan
J
J
Gellin
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003360:Cosmids; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D006822:Hybrid Cells / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012377:Rodentia; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers