Clustered localization of oligomeric Nef protein of human immunodeficiency virus type 1 on the cell surface.
We studied human immunodeficiency virus type 1 (HIV-1) Nef protein biochemically and histologically. HIV-1 Nef, derived from baculosystem and from cells infected with HIV-1, formed homomeric monomers, dimers, trimers, and further polymers. These oligomers were non-covalently associated. In cells infected with HIV-1, Nef molecules were clustered at the cell surface as well as cytoplasm. Our previous results have indicated that the Nef on the surface of cells infected with HIV-1 is cytotoxic against uninfected CD4+ T cells. Thus, it is very likely that the HIV-1-mediated cytotoxic reaction is due, at least in part, to the clustered localization of oligomeric Nef on the cell surface.
Institute for Laboratory Animal Research, Nagoya University School of Medicine, Japan.
Y
Y
Fujii
K
K
Otake
Y
Y
Fujita
N
N
Yamamoto
Y
Y
Nagai
M
M
Tashiro
A
A
Adachi
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D019084:Fluorescent Antibody Technique, Indirect; D015693:Gene Products, nef / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D018411:Spodoptera; D014162:Transfection; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus