Phospholipases involved in lysophosphatidylinositol metabolism in rat brain.
A phospholipase C activity highly specific for lysophosphoinositide (lysoPI-PLC) has been demonstrated to be present in synaptic plasma membranes of the rat brain. Several lines of evidence suggested that the lysoPI-PLC is an enzyme distinct from known isoforms of phosphoinositide-specific phospholipase C (PI-PLC). On the other hand, the occurrence of a Ca(2+)-independent phospholipase A1 hydrolyzing PI in rat brain was also demonstrated. The lysoPI-PLC hydrolyzed the 2-acyl isomer as well as the 1-acyl isomer of lysoPI. These findings suggest possible pathways for PI metabolism through lysoPI to yield monoacylglycerol (mainly 2-arachidonoyl glycerol) and inositolphosphates in the brain, which are different from the well-characterized PI-PLC pathway.
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan. tetkoba@phar.nagoya-cu.ac.jp
T
T
Kobayashi
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M
Kishimoto
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H
Okuyama
D000818:Animals; D001923:Brain Chemistry; D008246:Lysophospholipids / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054466:Phospholipases A1; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*
D008246:Lysophospholipids; C025449:lysophosphatidylinositol; D010741:Phospholipases A; D054466:Phospholipases A1; D010738:Type C Phospholipases