Expression of hybrid isomyosins in human skeletal muscle.
Myosin of human skeletal muscles was analyzed by means of several electrophoretic techniques. Myosin heavy chain (HC)-IIa-and HC-IIb-based isomyosins were identified by pyrophosphate-polyacrylamide gel electrophoresis (PP-PAGE). The electrophoretic mobilities of these fast-twitch muscle isomyosins differed in the order HC-IIa triplets < HC-IIb triplets. To determine the subunit composition of myosin molecules that function in intact muscle, two-dimensional electrophoresis in which the first and second dimensions were PP-PAGE and sodium dodecyl sulfate-PAGE, respectively, was also performed. Slow-twitch muscle isomyosin contained, in addition to slow-twitch light chain (LC) and HC-I isoforms, appreciable amounts of LC-2f, HC-IIa, and HC-IIb isoforms, and fast-twitch muscle isomyosin consisted of LC-2s and HC-I isoforms as well as fast-twitch LC and HC isoforms. Without consideration of HC- and slow-twitch alkali LC heterodimers, at least 31 possible isomyosins are derived from these findings on the subunit composition of isomyosins in human skeletal muscle.
Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
M
M
Wada
T
T
Okumoto
K
K
Toro
K
K
Masuda
T
T
Fukubayashi
K
K
Kikuchi
S
S
Niihata
S
S
Katsuta
D011756:Diphosphates; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry*; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000201:enzymology*; D018995:Myosin Heavy Chains / Q000737:chemistry*; D018994:Myosin Light Chains / Q000737:chemistry*
D011756:Diphosphates; D007527:Isoenzymes; D018994:Myosin Light Chains; D018995:Myosin Heavy Chains