Persistence of high virus neutralizing antibody titers in cats experimentally infected with feline immunodeficiency virus.
The development of virus neutralizing (VN) antibody is one of the most effective host defense mechanisms against virus infection. In the present study, we developed a new VN assay against feline immunodeficiency virus (FIV) using a feline T-lymphoblastoid cell line, MYA-1 cells, based on inhibition of viral reverse transcriptase production. This assay is applicable to strains of FIV which can not infect CRFK cells. By using the assay, we examined long-term responses of VN antibody in cats experimentally infected with FIV. VN antibody titers increased progressively during first 30 weeks post inoculation and remained at high titers thereafter for 7 years of observation periods.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Y
Y
Inoshima
Y
Y
Ikeda
M
M
Kohmoto
M R
MR
Pecoraro
M
M
Shimojima
Y
Y
Shimojima
G
G
Inada
Y
Y
Kawaguchi
K
K
Tomonaga
T
T
Miyazawa
T
T
Mikami
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control; D016089:Immunodeficiency Virus, Feline / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D007668:Kidney / Q000166:cytology / Q000821:virology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D013997:Time Factors
D000914:Antibodies, Viral; D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase