Biased expression of T cell receptor genes characterizes activated T cells in multiple sclerosis cerebrospinal fluid.
To better characterize the inflammatory response that occurs in the nervous system in multiple sclerosis (MS), T-cell receptor (TCR) gene expression was quantified from cerebrospinal fluid (CSF) cells of 21 patients with active disease. Unstimulated CSF cells expressed each of 22 different TCR beta chain variable region (V beta) gene families in proportion to their expression in simultaneously sampled peripheral blood. When CSF cells from individuals with MS were expanded by in vitro culture in T-cell growth factor/interleukin 2 and 4-containing medium (TCGF/IL2/IL4), restricted numbers of V beta genes were expressed. In many subjects, expanded CSF cells expressed predominantly V beta 2. In contrast to CSF, expansion of corresponding peripheral blood mononuclear cells (PBMC) in TCGF/IL2/IL4 resulted in persistent expression of all V beta gene families. Within individuals, different V beta genes were overexpressed by PBMC compared with CSF cells. No effect of the HLA haplotype of the individual on CSF V beta gene expression was observed. Expanded CSF cells retained their capacity to respond to mitogen stimulation, but the proliferative response to myelin basic protein (MBP) was not enhanced. Finally, freshly obtained CSF cells stimulated directly with MBP also expressed a limited number of V beta genes, although these were generally different from patterns observed following stimulation with TCGF/IL2/IL4. Thus, restricted populations of T cells capable of responding to TCGF/IL2/IL4, presumably reflecting in vivo activated cells, are compartmentalized in the nervous system in MS.
Department of Neurology, University of California, San Francisco, USA.
K
K
Usuku
N
N
Joshi
C J
CJ
Hatem
M A
MA
Wong
M C
MC
Stein
S L
SL
Hauser
D000328:Adult; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008875:Middle Aged; D005810:Multigene Family; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell