Effects of a new clerodane diterpenoid isolated from propolis on chemically induced skin tumors in mice.
Propolis is a resinous material gathered by honey bees from the buds and bark of certain trees and plants, and used inside their hives. Characteristic components of propolis are many kinds of flavonoid aglycones. The methanol extract of a Brazilian propolis was fractionated by HPLC, and a tumoricidal substance was isolated and characterized as a new clerodane diterpenoid (PMS-1) with a molecular formula of C20H32O3 (MW: 320). We investigated the effects of PMS-1 on skin tumorigenesis and the development of skin tumors induced by 7,12-dimethylbenz(a)anthracene application on mouse back skin. It was tentatively concluded that PMS-1 reduced the incidence of skin tumors by inhibition of DNA synthesis in a de novo pathway, and suppressed the growth of the tumors by decreasing DNA synthesis in a salvage pathway.
Mitamura
T
T
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Matsuno
T
T
Sakamoto
S
S
Maemura
M
M
Kudo
H
H
Suzuki
S
S
Kuwa
K
K
Yoshimura
S
S
Sassa
S
S
Nakayama
T
T
Nagasawa
H
H
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control; D010212:Papilloma / Q000139:chemically induced / Q000517:prevention & control; D011429:Propolis / Q000737:chemistry*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C102203:PMS-1; D015127:9,10-Dimethyl-1,2-benzanthracene; D011429:Propolis