Study of the changes of serum hyaluronic acid during porcine liver transplantation: influence of warm ischemia.
Twelve porcine liver transplantations were performed to investigate whether serum hyaluronic acid (HA) serves as a marker of warm ischemic injury. Group 1 was a control without warm ischemia (n = 7), and pigs in Group 2 were sacrificed by intracardiac KCl injection 60 min before harvesting (n = 5). All pigs survived more than 4 days in Group 1. In Group 2, all died within 2 days due to graft failure. Arterial and hepatic venous glutamic-oxaloacetic transaminase (GOT) in Group 2 were higher after revascularization. However, there were no differences between the 2 groups in arterial and hepatic venous HA levels. HA clearance by the graft also showed no differences between the groups. Although GOT reflected the degree of warm ischemia, HA and its hepatic clearance were not influenced by warm ischemic damage. In conclusion, HA was not thought to serve as a marker of liver injury when the graft suffered from warm ischemia.
First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Y
Y
Nakajima
J
J
Kimura
H
H
Isai
M
M
Tamura
K
K
Ito
H
H
Kon
H
H
Kamachi
M
M
Koike
K
K
Kusumoto
J
J
Uchino
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005260:Female; D006321:Heart / Q000187:drug effects; D006503:Hepatic Veins / Q000201:enzymology; D006820:Hyaluronic Acid / Q000097:blood*; D016031:Liver Transplantation / Q000009:adverse effects* / Q000276:immunology / Q000401:mortality; D008297:Male; D011189:Potassium Chloride / Q000008:administration & dosage / Q000633:toxicity; D015427:Reperfusion Injury / Q000378:metabolism / Q000401:mortality / Q000503:physiopathology*; D013552:Swine; D014019:Tissue Donors
D011189:Potassium Chloride; D006820:Hyaluronic Acid; D001219:Aspartate Aminotransferases