GLUT1-mediated glucose transport and its regulation by IGF-I in cultured bovine chromaffin cells.
Regulation of glucose transport was studied in primary cultures of bovine chromaffin cells (BCC) using the glucose analogue 2-deoxyglucose (DOG) as a model substrate. The glucose transporter in freshly isolated and cultured BCC was identified as GLUT1 by Western immunoblots. The level of GLUT1 increased by time in culture and was followed by an enhancement in uptake of DOG. The DOG uptake was stimulated by insulin-like growth factor I (IGF-I) with an EC50 of 1 nM and a maximal response (approximately 2-fold) was obtained at 10-100 nM IGF-I. Insulin was at least 100-fold less potent than IGF-I. Exposure to 10(-8) M IGF-I also caused a redistribution of GLUT1 from an intracellular compartment to a plasma membrane-enriched fraction. Our results demonstrate a GLUT1-mediated glucose uptake in adrenomedullary cells. An enhanced glucose transport in response to IGF-I appears to be coupled to activation of IGF receptor type 1 and GLUT1 translocation.
Fladeby
C
C
Department of Physiology, University of Bergen, Norway.
Bjønness
B
B
Serck-Hanssen
G
G
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western; D002417:Cattle; D019439:Chromaffin Cells / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D017526:Receptor, IGF Type 1 / Q000378:metabolism
D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D007334:Insulin-Like Growth Factor I; D003847:Deoxyglucose; D017526:Receptor, IGF Type 1; D005947:Glucose