Proinflammatory cytokines and their corresponding receptor proteins in eccrine sweat glands in normal and cutaneous leishmaniasis human skin. An immunohistochemical study.
Paraformaldehyde-fixed biopsy specimens of normal and chronic cutaneous leishmaniasis human skin were investigated for the presence and cellular distribution of interleukin-1 alpha, interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha and the corresponding receptors in eccrine sweat glands, using an indirect immunoperoxidase technique. There was cytoplasmic staining for all 4 cytokines as well as their receptor proteins in the clear cells of the eccrine sweat glands of both normal and inflamed skin specimens. No staining could be seen in the dark cells or the myoepithelial cells, neither in normal nor in inflamed skin. However, a difference between normal and inflamed skin was observed in the ductal system. Thus, cell layers of the dermal ducts in leishmaniasis skin were stained for all 4 cytokines, with more intense labelling in the basal cell layer of the coiled ducts, while in the normal skin, an intense staining was more evident in the inner luminal layer, with variable and less intense labelling of the basal layer. The immunolabelling for the cytokine receptors within the dermal ducts exhibited similar staining intensity in both luminal and basal cell layers, except in the case of the IL-6 receptor, which showed a moderate to intense signal in the basal cell layer but a weak staining of the luminal cell layer. Infiltrating inflammatory cells around the sweat gland apparatus in leishmaniasis skin exhibited immunoreactivities for all cytokines and their corresponding receptors.
Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
A A
AA
Ahmed
K
K
Nordlind
M
M
Schultzberg
S
S
Lidén
D000328:Adult; D000368:Aged; D001706:Biopsy; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism*; D004439:Eccrine Glands / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016773:Leishmaniasis, Cutaneous / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018121:Receptors, Cytokine / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
D016207:Cytokines; D018121:Receptors, Cytokine