Specific photoaffinity labelling of a ferripyoverdin outer membrane receptor of Pseudomonas aeruginosa.
In order to identify and characterize the receptors involved in pyoverdin-mediated iron transport in Pseudomonas aeruginosa ATCC 15692, a photoactivatable siderophore has been synthesized. In the dark, this probe is stable and is able to promote iron transport at the same rate as the native pyoverdin. Under irradiation at 312 nm, the molecule is photodecomposed and a clear inhibition of the iron transport is observed. With the radioactive form of this photoactivatable probe, we were able to visualize on a SDS-PAGE gel a labelled protein of approximately 90 kDa molecular mass, which is very likely the FpvA receptor or a yet unknown pyoverdin receptor.
Laboratoire de Chimie Microbienne, Faculté de Chimie, Université Louis Pasteur, Strasbourg, France.
A
A
Ocaktan
I
I
Schalk
C
C
Hennard
C
C
Linget-Morice
P
P
Kyslik
A W
AW
Smith
P A
PA
Lambert
M A
MA
Abdallah
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001386:Azides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017136:Ion Transport; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D009842:Oligopeptides; D010860:Pigments, Biological / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism; D017262:Siderophores / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays
D000345:Affinity Labels; D001386:Azides; D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D007501:Iron