Characterization of ion interference in gossypol consumption.
The goal of the present investigation was to show that gossypol has the potential of ion interference capable of affecting the metabolic activation of the biologic system. Gossypol was administered orally at 20 mg/kg body weight (bw) for 8 weeks and the animals were fed a controlled diet. At the end of the experiment, liver supernatant was subjected to atomic absorption spectrometry. The characteristic ion interferences were examined for calcium, ferrous, sodium, magnesium, potassium and chloride ions. Glutathione level and lactate dehydrogenase activity were also monitored. The peak heights for calcium and ferrous ions were high and low respectively but the ion ratio for calcium is suggestive of a ligand-like substance that may be cumulative, thereby leading to biologic magnification (toxicity).
Achudume
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Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
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D000818:Animals; D002118:Calcium / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013054:Spectrophotometry, Atomic
D002712:Chlorides; D005296:Ferrous Compounds; D012964:Sodium; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D008274:Magnesium; D006072:Gossypol; D011188:Potassium; D002118:Calcium