Isolation and mapping of a new set of 129 RFLP markers in Arabidopsis thaliana using recombinant inbred lines.
A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences of P1 clones. Dominant/null alleles affecting low-copy number sequences account for nine of the mapped polymorphisms, suggesting that deletions are not rare in A. thaliana. Recombinant inbred (RI) lines were used for mapping these marker loci. RI line-based mapping allows integration of this set of markers with markers previously reported as well as with any markers mapped in the future using this replenishable mapping resource. These markers are useful for map-based gene isolation and genome physical mapping in A. thaliana as well as studies of chromosome colinearity (synteny) with related species.
Mitsui Plant Biotechnology Research Institute, Tsukuba, Japan.
Y G
YG
Liu
N
N
Mitsukawa
C
C
Lister
C
C
Dean
R F
RF
Whittier
D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D008040:Genetic Linkage; D005819:Genetic Markers; D015698:Genomic Library; D012150:Polymorphism, Restriction Fragment Length; D012641:Selection, Genetic
D004274:DNA, Recombinant; D005819:Genetic Markers