A protein tyrosine kinase associated with the ATP-dependent inactivation of adipose diacylglycerol acyltransferase.
The activity that has been previously reported to reversibly inactivate adipose glycerolphosphate acyltransferase (GPAT) and diacylglycerol acyltransferase (DGAT) in vitro in the presence of ATP is shown here to be partially purified from adipose tissue with an apparent molecular weight of 68 kDa. The activity responsible for inactivating DGAT is associated with a kinase activity as determined by phosphate incorporation both into microsomal proteins and into a synthetic tyrosine-containing peptide as substrate for protein tyrosine kinase. Two microsomal polypeptides of 53 and 69 kDa are major substrates of this kinase. Both DGAT inactivating and kinase activities assayed from the purified sample have been found to be insensitive to the Ser/Thr kinase inhibitor H-7 while being sensitive to genistein and tyrphostin-25. A crude protein phosphatase preparation from liver was capable of reversing the effects of both activities. The purified sample was also shown to inactivate GPAT in the presence of ATP. These results suggest that a protein tyrosine kinase, in concert with a protein tyrosine phosphatase, may regulate the activities of DGAT and GPAT by a phosphorylation-dephosphorylation mechanism.
Department of Biochemistry, University of Ottawa, Ontario, Canada.
T E
TE
Lau
M A
MA
Rodriguez
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002847:Chromatography, Agarose; D051048:Diacylglycerol O-Acyltransferase; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000037:antagonists & inhibitors*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D019319:Okadaic Acid / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012969:Sodium Fluoride / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D004791:Enzyme Inhibitors; D010710:Phosphates; D019319:Okadaic Acid; D014638:Vanadates; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D004492:Edetic Acid; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D051048:Diacylglycerol O-Acyltransferase; D011505:Protein-Tyrosine Kinases; D010749:Phosphoprotein Phosphatases