RNA-mediated transformation in Aspergillus nidulans recovers gene functions lost by deletion or by point mutations.
This work presents the results on two different approaches of RNA-mediated transformation in A. nidulans: a) the receptor strain was an argB2 (III) mutant deficient in arginine (OTCase deficient), and b) the receptor was an A. nidulans mutant defective in nitrate reductase synthesis due to a deletion in the niaD gene (VIII). The analyses of the arg+ and the nia+ retrotransformants allowed an insight on the fate and inheritance of the newly acquired characteristics. The occurrence and the study of Gene Inactivation Mechanism (RIP-like) inactivating the expression of extra copies of genes ectopically scattered over the receptor genome, was a byproduct of this research. Retrotransformants were also used as RNA-donor for a second turn of retrotransformation of the argB and niaD receptor strains. Genetic analyses of the new retrotransformants proved that the retrotransformation ability is kept by the re-extracted RNA when used in a second round of transformation process. This is the best genetic evidence that the newly acquired genetic characteristics were cDNA inserted, precisely transcribed and expressed. These are the first in vivo evidences of genetic information transference mediated by homologous RNA in lower eukaryotes.
Zucchi
T M
TM
Department of Parasitology, University of São Paulo, Brazil.
D001233:Aspergillus nidulans / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D017353:Gene Deletion; D016681:Genome, Fungal; D017354:Point Mutation; D012331:RNA, Fungal / Q000235:genetics*
D018076:DNA, Complementary; D012331:RNA, Fungal