Biphasic and dose-dependent accumulation of INSP3 in Trypanosoma cruzi stimulated by a synthetic peptide carrying a chicken alpha D-globin fragment.
A synthetic peptide corresponding to residues 1 to 40 of chicken alpha D-globin stimulated the accumulation of inositol phosphates (InsPs) in Trypanosoma cruzi epimastigotes. This accumulation was shown to be biphasic and dose-dependent. The enhanced inositol trisphosphate (InsP3) accumulation occurred at the third minute with a subsequent increase which is not transient, but sustained over a much longer period. Another peptide carrying residues 35 to 73 was less active. These results evidenced the activation of the phosphoinositide phospholipase C activity in response to peptides that bind specifically to T. cruzi epimastigote cells.
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
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MN
Garrido
M I
MI
Bollo
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EE
Machado-Domenech
D000818:Animals; D002645:Chickens; D005914:Globins / Q000737:chemistry; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D014349:Trypanosoma cruzi / Q000378:metabolism*
D010455:Peptides; D015544:Inositol 1,4,5-Trisphosphate; D005914:Globins