Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs.
Analysis of polyA-selected RNAs and cDNA clones from several human cell lines revealed the presence of a 93-bp exon in the PI kinase homology region of DNA-PKcs that was not present in the cDNA sequence derived from HeLa cell cDNA clones. RT-PCR showed that most DNA-PKcs mRNAs in human cells have this exon. Thus, the nascent DNA-PKcs polypeptide is composed of 4127 aa and has a predicted mol. wt. of 469021 (470 kDa).
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.
M A
MA
Connelly
H
H
Zhang
J
J
Kieleczawa
C W
CW
Anderson
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D005091:Exons / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016366:Open Reading Frames / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases