New techniques for making whole-cell recordings from CNS neurons in vivo.
Patch-type pipettes increasingly are being used to obtain intracellular 'whole-cell' recordings from neurons. Here we describe our methods for making whole-cell recordings in vivo from midbrain neurons in an electric fish. Novel elements in the procedure are: A device for micropositioning the pipette when near a cell, use of a 'Picospritzer' for cleaning the pipette tip and cell surface, and an electroporetic method for perforating the patch following seal formulation. In addition, we show that extracellular and intracellular recordings can be made from the same neuron. Stable intracellular recordings can be made from neurons at least as small as 10 microns.
Department of Biology, University of Utah, Salt Lake City 84112, USA.
G J
GJ
Rose
E S
ES
Fortune
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004555:Electric Fish; D004558:Electric Stimulation; D008636:Mesencephalon / Q000166:cytology*; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*