Butyrate enhances major histocompatibility complex class I, HLA-DR and ICAM-1 antigen expression on differentiated human intestinal epithelial cells.
Besides its metabolic role, butyrate, a by-product of colonic fermentation, can modulate colonocyte proliferation and the expression of various molecules. In inflammatory bowel diseases, epithelial cells express HLA class II molecules and may behave as antigen-presenting cells. This study was performed to characterize the effect of butyrate on major histocompatibility complex expression by human colonocytes in comparison with interferon-gamma. Five cell lines displaying different differentiation features were analysed for antigen expression by flow cytofluorimetry. All lines expressed class I antigens, whereas only SW 1116 cells express HLA-DR. On these cells, butyrate and interferon-gamma strongly enhanced HLA-DR and ICAM-1 expression, whereas a mild increase in class I antigens was noted. Moreover, an increase in class I antigens was observed on two other differentiated cell lines, and it was synergistic with interferon-gamma. Butyrate, by its modulation of HLA-DR, ICAM-1 and HLA class I expression, may act on antigen presentation and, thus, influence some inflammatory processes.
Centre de Recherche en Nutrition Humaine, Hôpital G&R Laënnec, Nantes, France.
S
S
Siavoshian
H M
HM
Blottière
N
N
Bentouimou
C
C
Cherbut
J P
JP
Galmiche
D000085:Acetates / Q000494:pharmacology; D000943:Antigens, Differentiation; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D008285:Major Histocompatibility Complex; D011422:Propionates / Q000494:pharmacology; D014158:Transcription, Genetic
D000085:Acetates; D000943:Antigens, Differentiation; D002087:Butyrates; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D011422:Propionates; D020148:Butyric Acid; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma