Effect of maternal ethanol consumption on maternal and fetal calcium metabolism.
Alcohol consumption can have deleterious effects on both adult and developing bone. The mechanism(s) by which alcohol affects bone, however, is unknown. This study investigated the possibility that alcohol affects bone by alterations in calcium (Ca) metabolism. Female rats were fed lab chow ad libitum (C, Control) or a liquid diet with (E, Ethanol) or without (PF, Pair-Fed) ethanol. After 2 weeks on their respective diets, the rats were bred and the experimental diets continued throughout gestation. Blood (dams only) and tissue were collected on day 21 of gestation. The Ca content of maternal bone showed a trend toward a decrease in E and PF compared with C dams. Ionic Ca (iCa) levels were decreased in the blood of the E compared with PF and C dams. Serum parathyroid hormone levels were elevated in the E compared with C dams, consistent with the low iCa levels. Serum levels of 1,25(OH)2D, however, were elevated only in the PF dams. Mean fetal body weight and fetal skeletal ossification were reduced in the E compared with PF and C groups, but no group differences were found in fetal Ca content. These results indicate that maternal ethanol consumption compromised the ability of the dam to regulate her blood iCa levels, possibly partly due to a failure to increase 1,25(OH)2D levels. The delays in skeletal development observed in the ethanol exposed fetuses, however, do not appear to result from impaired placental Ca transfer.
Department of Anatomy, University of British Columbia, Vancouver, Canada.
K
K
Keiver
L
L
Herbert
J
J
Weinberg
D000818:Animals; D001846:Bone Development / Q000187:drug effects / Q000502:physiology; D002116:Calcitonin / Q000502:physiology; D002118:Calcium / Q000097:blood*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000503:physiopathology*; D008431:Maternal-Fetal Exchange / Q000187:drug effects* / Q000502:physiology; D015675:Osteocalcin / Q000502:physiology; D010281:Parathyroid Hormone / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014807:Vitamin D / Q000502:physiology
D010281:Parathyroid Hormone; D015675:Osteocalcin; D014807:Vitamin D; D000431:Ethanol; D002116:Calcitonin; D002118:Calcium