Catalytic role of the alpha-carboxylate of the Glu residue of glutathione in glutathione S-transferases.
The recently proposed catalytic role of the alpha-carboxylate of the Glu residue of glutathione in glutathione S-transferases (Widersten et al, Biochemistry 35, 7731-7742 (1996)) was examined. Based on structural considerations, it is clear that conformational changes in both glutathione and glutathione S-transferase are required. Recent kinetic studies by Ricci and coworkers (Ricci et al, J. Biol. Chem. 271, 16187-16192 (1996) and Caccuri et al, J. Biol. Chem. 271, 16193-16198 (1996)) may provide the missing evidence for these conformational changes. Possible ways to test this hypothesis are discussed.
Zheng
Y J
YJ
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. vg857@dewar.pnl.gov
Ornstein
R L
RL
D002264:Carboxylic Acids / Q000737:chemistry*; D002384:Catalysis; D018698:Glutamic Acid / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D002264:Carboxylic Acids; D018698:Glutamic Acid; D005982:Glutathione Transferase; D005978:Glutathione