D2-like dopamine receptors are not detectable on human peripheral blood lymphocytes.
The binding of [3H]-nemonapride to human peripheral blood lymphocytes (PBL) was studied using various competing ligands specific for D2-like dopamine receptors. There is no detectable stereoselectivity for the stereoisomers of butaclamol, and competitions with haloperidol and sulpiride also show no evidence of specific binding to D2-like dopamine receptors. RT-PCR of RNA from human lymphocytes showed that there is no detectable D2 mRNA (even with nested PCR). D3 mRNA was, however, detectable by RT-PCR, but only at low levels that could not be detected by Northern blots of PBL total RNA.
Research School of Biosciences, University, Canterbury, Kent, UK.
J M
JM
Vile
P G
PG
Strange
D000328:Adult; D014150:Antipsychotic Agents / Q000493:pharmacokinetics; D001549:Benzamides / Q000493:pharmacokinetics; D001667:Binding, Competitive; D018492:Dopamine Antagonists / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011869:Radioligand Assay; D017448:Receptors, Dopamine D2 / Q000032:analysis* / Q000235:genetics; D050637:Receptors, Dopamine D3; D012559:Schizophrenia / Q000097:blood / Q000188:drug therapy
D014150:Antipsychotic Agents; D001549:Benzamides; C493593:DRD3 protein, human; D018492:Dopamine Antagonists; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; C030265:nemonapride