Identification of BIME as a subunit of the anaphase-promoting complex.
The initiation of anaphase and exit from mitosis require the activation of a proteolytic system that ubiquitinates and degrades cyclin B. The regulated component of this system is a large ubiquitin ligase complex, termed the anaphase-promoting complex (APC) or cyclosome. Purified Xenopus laevis APC was found to be composed of eight major subunits, at least four of which became phosphorylated in mitosis. In addition to CDC27, CDC16, and CDC23, APC contained a homolog of Aspergillus nidulans BIME, a protein essential for anaphase. Because mutation of bimE can bypass the interphase arrest induced by either nimA mutation or unreplicated DNA, it appears that ubiquitination catalyzed by APC may also negatively regulate entry into mitosis.
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
J M
JM
Peters
R W
RW
King
C
C
Höög
M W
MW
Kirschner
D000595:Amino Acid Sequence; D000705:Anaphase; D000818:Animals; D001230:Aspergillus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D010063:Ovum; D010766:Phosphorylation; D044767:Ubiquitin-Protein Ligases; D014982:Xenopus laevis
C065697:BIME protein, Emericella nidulans; D018797:Cell Cycle Proteins; D016213:Cyclins; D005656:Fungal Proteins; D044767:Ubiquitin-Protein Ligases; D008025:Ligases