A pig collagen peptide fraction. A unique material for maintaining biological activity during lyophilization and during storage in the liquid state.
There is frequent use of human and animal proteins as stabilizers during lyophilization of a variety of biological substances with a view to long term stable storage. This report describes the comparative excellent stabilizing effect of a porcine collagen peptide fraction (CPF) during the lyophilization and subsequent storage of three commonly used biological substances, alkaline phosphatase, tissue plasminogen activator and thrombin. The CPF was heated to 150 degrees C for one hour before use. The CPF was shown to have some advantage during lyophilized storage over human serum albumin. Solutions of thrombin stored in CPF at room temperature and at 37 degrees C for one week retained nearly all activity, while storage of thrombin in human serum albumin solution at 37 degrees C lost nearly all biological activity. These preliminary data suggest that porcine CPF is a safe and advantageous stabilizer for addition to biological products with a view to long-term lyophilized storage and short-term liquid storage.
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK.
P J
PJ
Gaffney
T A
TA
Edgell
P A
PA
Dawson
A W
AW
Ford
E
E
Stocker
D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D004355:Drug Stability; D004356:Drug Storage; D005612:Freeze Drying; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D013552:Swine; D013696:Temperature; D013917:Thrombin / Q000737:chemistry* / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000737:chemistry* / Q000494:pharmacology
D010455:Peptides; D011994:Recombinant Proteins; D003094:Collagen; D000469:Alkaline Phosphatase; D013917:Thrombin; D010959:Tissue Plasminogen Activator