Synthesis and chromatographic characterization of dextran-coated zirconia high-performance liquid chromatographic stationary phases.
Porous zirconia particles made by the oil emulsion (OE) method and the polymerization-induced colloid aggregation (PICA) method have been coated with a small, carboxymethylated (approximately 5%) dextran polymer and crosslinked in place. The parameters of the coating process (dextran concentration, adsorption time and crosslinker concentration) have all been examined and an optimum value for each determined. The coated and uncoated materials were characterized by nitrogen sorptometry and size-exclusion chromatography (SEC) using solutes (polystyrenes and dextrans) of well-defined molecular masses. Nitrogen sorptometry results show that the PICA material has a much lower pore volume and smaller pore diameter than do the OE materials. Despite this, the elution volumes of the SEC probes change very little upon polymer coating the PICA material while the OE material shows a very large change upon coating.
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
C J
CJ
Dunlap
P W
PW
Carr
D000327:Adsorption; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D003432:Cross-Linking Reagents; D003911:Dextrans / Q000737:chemistry*; D004655:Emulsions; D007700:Kinetics; D010316:Particle Size; D013499:Surface Properties; D013816:Thermodynamics; D015040:Zirconium / Q000737:chemistry*
D003432:Cross-Linking Reagents; D003911:Dextrans; D004655:Emulsions; D015040:Zirconium; C028541:zirconium oxide