Modification of the radiosensitivity of human cells to which simian virus 40 T-antigen was transfected.
Effects of the introduction of the Simian virus 40 T-antigen (SV40 T-Ag) gene to cultured human cells were examined in relation to radiosensitivity. Two relatively radioresistant tumor cell lines (T98 and G361) became significantly radiosensitive after the introduction of SV40 T-Ag, whereas radiosensitive tumor cell lines did not show a change in radiosensitivity. In contrast, a human fibroblast cell line became radioresistant after SV40 T-Ag introduction. T98 cells which have a mutation at codon 237 in the p53 gene were unable to form a complex between p53 protein and SV40 T-Ag, whereas G361, which became radiosensitive by a SV40 T-Ag introduction, formed the complex. This indicates that the status of p53 is independent of the change in radiosensitivity in the cell lines studied.
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
N
N
Yamagishi
J
J
Miyakoshi
S
S
Ohtsu
R S
RS
Day
H
H
Takebe
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D005347:Fibroblasts; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming