In-vitro activity of macrolides alone and in combination with artemisin, atovaquone, dapsone, minocycline or pyrimethamine against Cryptosporidium parvum.
The anticryptosporidial activity of four macrolides alone and in combination with other antimicrobial agents was investigated against ten clinical isolates of Cryptosporidium parvum recovered from stools of AIDS patients. The susceptibility tests were performed by inoculation of the protozoa on to cell monolayers and determining the parasite count after 72 h incubation at 37 degrees C. The culture medium was supplemented with Dulbecco's modified Eagle's medium containing serial dilutions of azithromycin, clarithromycin, roxithromycin, spiramycin, alone or in combination with artemisin, atovaquone, dapsone, minocycline or pyrimethamine. Most of the agents had an inhibitory effect on parasite growth, but only at high concentrations. No agent was able to inhibit parasite growth completely, even at the highest concentrations used. The more effective agents, azithromycin, clarithromycin, roxithromycin, minocycline and pyrimethamine, produced no more than a 13.1-27.8% reduction in oocyst count and no more than a 15.1-35.7% in schizont count. Positive interaction was clearly demonstrated when macrolides were tested in combination with minocycline or pyrimethamine.
Institute of Infectious Diseases and Public Health, University of Ancona, Italy.
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Giacometti
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Cirioni
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Scalise
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000469:parasitology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D037621:Artemisinins; D053626:Atovaquone; D003457:Cryptosporidiosis / Q000150:complications / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000187:drug effects*; D003622:Dapsone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D018942:Macrolides; D008911:Minocycline / Q000494:pharmacology*; D009285:Naphthoquinones / Q000494:pharmacology*; D011739:Pyrimethamine / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D037621:Artemisinins; D007783:Lactones; D018942:Macrolides; D009285:Naphthoquinones; D012717:Sesquiterpenes; D003622:Dapsone; D008911:Minocycline; C075875:artemisin; D053626:Atovaquone; D011739:Pyrimethamine