Overexpression of E2F1 in astrocytes leads to neoplastic transformation and changes in expression of retinoblastoma family members.
Overexpression of mouse E2F1 full-length but not truncated forms results in neoplastic transformation of astrocytes in vitro. This neoplastic transformation is accompanied with changes in cell morphology and expression of cell cycle regulators. Transformed astrocytes have higher expression of cdk2, pRb, and p107 than control astrocytes. However, expression of glial fibrillary acidic protein (GFAP) and p130 is reduced in transformed astrocytes.
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
M
M
Miyajima
H O
HO
Nornes
K
K
Sato
T
T
Neuman
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015153:Blotting, Western; D042846:CDC2-CDC28 Kinases; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D005134:Eye Neoplasms / Q000378:metabolism*; D016161:Genes, Retinoblastoma / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009457:Neuroglia / Q000378:metabolism; D010957:Plasmids; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis; D012175:Retinoblastoma / Q000378:metabolism*; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000096:biosynthesis*; D014162:Transfection; D014407:Tumor Cells, Cultured
C533431:Arid4a protein, mouse; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; D009363:Neoplasm Proteins; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases