Processing of kaliuretic peptide in human plasma and serum.
High performance-gel permeation chromotography (HP-GPC) evaluation of human plasma and serum incubated at 37 degrees C for 0, 0.5, 1, 1.5, 2, 4, 8, and 24 h after the addition of the human synthetic form of kaliuretic peptide revealed only a peak where the pure synthetic form of kaliuretic peptide elutes and that this peak decreased by 60% or greater in plasma-EDTA, plasma-heparin, and serum within 30 min. The metabolic processing of kaliuretic peptide in plasma-EDTA, plasma-heparin, and serum, respectively, was complete in 2 h with only 4%, 1%, and 2% of kaliuretic peptide's originally added concentration being still present.
Department of Medicine, James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
R M
RM
Overton
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Vesely
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D011498:Protein Precursors / Q000097:blood* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011863:Radioimmunoassay
D011498:Protein Precursors; C075322:atrial natriuretic factor precursor (79-98); D009320:Atrial Natriuretic Factor; D006493:Heparin