Isolation of viable sperm cells from tobacco (Nicotiana tabacum).
Viable sperm cells of Nicotiana tabacum were isolated by the semi-vivo technique. After pollination, excised styles were floated, cut end immersed, in a solution of 15% sucrose with 0.01% boric acid and 0.03% Ca(NO3)2 at 27 degrees C in a growth chamber until pollen tubes emerged. After sperm cells were formed (at least 8 h after pollination) tubes were immersed in a 9% mannitol solution. In this solution, sperm cells are nearly ellipsoidal and retain viability for over 6 h.
Cao
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Y
Department of Botany and Microbiology, University of Oklahoma, Norman 73019-0245, USA.
Reece
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A
Russell
S D
SD
D002470:Cell Survival; D046508:Culture Techniques / Q000379:methods*; D003593:Cytoplasm; D005452:Fluoresceins / Q000737:chemistry; D005453:Fluorescence; D008853:Microscopy; D010947:Plants, Toxic; D011058:Pollen / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D014026:Nicotiana / Q000502:physiology*
D005452:Fluoresceins; C018506:diacetylfluorescein