Isolation and characterisation of T lymphocytes from sural nerve biopsies in patients with Guillain-Barré syndrome and chronic inflammatory demyelinating polyneuropathy.
OBJECTIVE
To characterise cultured T lymphocytes from nerve biopsies in patients with Guillain-Barré syndrome and chronic inflammatory demyelinating polyneuropathy (CIDP).


METHODS
Sural nerve biopsies, obtained from six patients with Guillain-Barré syndrome, four with CIDP, and six controls with other neuropathies, were cultured with 20 U/ml recombinant interleukin-2 (IL-2) for eight weeks. Flow cytometry was used to determine the phenotype of cultured T lymphocytes. Their proliferative responses to a range of bacterial antigens were also examined.


RESULTS
T cell lines were established from four of six patients with Guillain-Barré syndrome, one of four with CIDP, one patient with peripheral nerve vasculitis, and none of five controls with non-inflammatory neuropathies. One of these T cell lines from a patient with Guillain-Barré syndrome, preceded by Campylobacter jejuni infection, consisted entirely of gamma delta TCR+ T lymphocytes. The peripheral blood of this patient also contained an increased frequency of gamma delta T cells when stimulated with C jejuni. The nerve derived T cell lines failed to show a proliferative response to bacterial antigens or to a preparation of myelin proteins.


CONCLUSIONS
A new technique to isolate T cells from nerve biopsies in patients with Guillain-Barré syndrome and CIDP is reported. This technique may prove to be a useful tool in the investigation of the pathogenesis of other inflammatory neuropathies such as peripheral nerve vasculitis. The isolation of a gamma delta TCR+ nerve T cell line is of interest because of the possibility that these cells might respond to glycolipid epitopes common to C jejuni and peripheral nerve gangliosides.
Centre for Clinical Research in Immunology and Signalling, Medical School, University of Birmingham, UK.
A
A
Ben-Smith
J S
JS
Gaston
P C
PC
Barber
J B
JB
Winer
D015703:Antigens, CD; D016123:Campylobacter jejuni / Q000302:isolation & purification; D018929:Cell Culture Techniques; D002465:Cell Movement; D003587:Cytomegalovirus / Q000472:pathogenicity; D003711:Demyelinating Diseases / Q000175:diagnosis* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011129:Polyradiculoneuropathy / Q000097:blood* / Q000209:etiology / Q000382:microbiology; D013497:Sural Nerve / Q000503:physiopathology*; D013601:T-Lymphocytes
D015703:Antigens, CD