Anti-glomerular basement membrane disease: role of enzyme-linked immunosorbent assays in diagnosis.
We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivity, 97.0% specificity). The second ELISA kit was much less sensitive (87.8% agreement, 63.3% sensitivity, 92.2% specificity). ELISA tests for the measurement of anti-GBM antibodies allow for quantitation and may have greater sensitivity than IFA. Sensitivity, however, may depend on the commercial test used.
Litwin
C M
CM
Department of Pathology, University of Utah, Salt Lake City 84132, USA.
Mouritsen
C L
CL
Wilfahrt
P A
PA
Schroder
M C
MC
Hill
H R
HR
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019867:Anti-Glomerular Basement Membrane Disease / Q000175:diagnosis*; D000906:Antibodies / Q000378:metabolism; D001485:Basement Membrane / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008875:Middle Aged
D000906:Antibodies