Regulation of sucrase and lactase in Caco-2 cells: relationship to nuclear factors SIF-1 and NF-LPH-1.
Recent studies suggest the importance of two transcription factors, Cdx-2 and NF-LPH-1, in the regulation of sucrase-isomaltase (SI) and lactase-phlorizin hydrolase (LPH) gene expression, respectively. Cdx-2 accounts for the tissue specificity of sucrase expression (16), and NF-LPH-1 varies with postnatal changes in lactase activity, suggesting a role in its developmental regulation (22). We used electrophoretic mobility shift assays to study the relationship of Cdx-2 and NF-LPH-1 to SI and LPH gene expression in Caco-2 cells to provide evidence regarding the role of these factors in the development of sucrase and lactase with cellular differentiation. We found that Cdx-2 levels correlated with SI expression and that NF-LPH-1 did not correlate with LPH expression. These studies suggest a role for Cdx-2 but not for NF-LPH-1 in the development of carbohydrase expression in these cells.
Gastroenterology Research Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin, USA.
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Olsen
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Lloyd
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Korsmo
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He
D000071616:CDX2 Transcription Factor; D018938:Caco-2 Cells; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000502:physiology*; D010694:Lactase-Phlorizin Hydrolase / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D013394:Sucrase-Isomaltase Complex / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators
D000071616:CDX2 Transcription Factor; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; D013394:Sucrase-Isomaltase Complex; D010694:Lactase-Phlorizin Hydrolase