The influence of sub-optimal protein nutrition on insulin hypersecretion evoked by high-energy/high-fat feeding in rats.
Low (8%) protein feeding during pregnancy impairs the functional development of the fetal endocrine pancreas. Continued low-protein feeding post-natally decreases pancreatic insulin content and secretion, whereas transfer to standard diet evoked beta-cell recuperation. Glucose-stimulated insulin secretion (GSIS) and insulin action were examined in vivo at 28 days after transfer from 8% protein diet to a high-energy/high-fat/standard (20%)-protein diet (HEF diet). HEF feeding dramatically enhanced GSIS after intravenous glucose in control rats, but not in rats previously maintained on the low-protein diet. However, glucose disappearance after intravenous glucose, and glucose production and whole-body glucose disposal during euglycaemic-hyperinsulinaemic clamps were unaffected by prior protein malnutrition. In conclusion, impaired insulin secretion after protein malnutrition is exacerbated by high-energy/high-fat feeding, but this response is not linked to enhanced whole-body insulin resistance.
Department of Biochemistry, Medical Sciences, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK.
M J
MJ
Holness
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002241:Carbohydrates / Q000494:pharmacology; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D015309:Glucose Clamp Technique; D007275:Injections, Intravenous; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D000078790:Insulin Secretion; D011247:Pregnancy; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D002241:Carbohydrates; D004041:Dietary Fats; D007328:Insulin; D011506:Proteins; D005947:Glucose