In vivo models of thrombosis for the antiphospholipid syndrome.
Utilizing this unique animal model of thrombosis we demonstrated that human (IgG, IgM or IgA) polyclonal and monoclonal antiphospholipid antibodies derived from APS patients have a significant enhancing effect on thrombus formation. This effect is reversed by treatment of the mice with hydroxychloroquine (plaquenil). In addition murine polyclonal and monoclonal anticardiolipin antibodies induced by active immunization with human beta 2-GP1 or human anticardiolipin antibodies showed to have thrombogenic properties in CD1 mice. Antibodies with antihuman beta 2-GP1 activity alone did not seem to affect thrombus formation.
Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, GA 30310-1495, USA.
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Pierangeli
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Harris
D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000502:physiology; D017152:Antibodies, Antiphospholipid / Q000502:physiology; D016736:Antiphospholipid Syndrome / Q000150:complications*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D051379:Mice; D013927:Thrombosis / Q000209:etiology*; D053482:beta 2-Glycoprotein I
D017153:Antibodies, Anticardiolipin; D017152:Antibodies, Antiphospholipid; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I