Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli.
An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity of the probe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, respectively.
Department of Bacterial Infections, Osaka University, Japan.
K
K
Nagayama
Z
Z
Bi
T
T
Oguchi
Y
Y
Takarada
S
S
Shibata
T
T
Honda
D000469:Alkaline Phosphatase; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D005069:Evaluation Studies as Topic; D010861:Fimbriae, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D015345:Oligonucleotide Probes; D012475:Salmonella / Q000235:genetics; D012680:Sensitivity and Specificity; D012760:Shigella / Q000235:genetics; D013045:Species Specificity; D014733:Vibrio / Q000235:genetics
D015345:Oligonucleotide Probes; D000469:Alkaline Phosphatase