DNA sequence recognition by hybridization to short oligomers: experimental verification of the method on the E. coli genome.
A newly developed method for sequence recognition by hybridization to short oligomers is verified for the first time in genome-scale experiments. The experiments involved hybridization of 15,328 randomly selected 2-kb genomic clones of Escherichia coli with 997 short oligomer probes to detect complementary oligomers within the clones. Lists of oligomers detected within individual clones were compiled into a database. The database was then searched using known E. coli sequences as queries. The goal was to recognize the clones that are identical or similar to the query sequences. A total of 76 putative recognitions were tested in two separate but complementary recognition experiments. The results indicate high specificity of recognition. Current and prospective applications of this novel method are discussed.
Milosavljević
A
A
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, Illinois 60439-4833, USA. amilosav@curagen.com
Savković
S
S
Crkvenjakov
R
R
Salbego
D
D
Serrato
H
H
Kreuzer
H
H
Gemmell
A
A
Batus
S
S
Grujić
D
D
Carnahan
S
S
Paunesku
T
T
Tepavcević
J
J
D001483:Base Sequence; D001704:Biopolymers; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D001704:Biopolymers; D004269:DNA, Bacterial