Mechanism of enhancement of renal (Na(+)+K+) ATPase activity following chronic ethanol exposure.
A method was devised to determine the nature of the mechanism of the increase in renal (Na(+)+K+)-ATPase in rats fed dilute ethanol for ten weeks. Antiserum to (Na(+)+K+)-ATPase obtained from rabbits was added to microsomal fractions of kidney and the activities of (Na(+)+K+)-ATPase and Mg2+ ATPase were determined. The addition of antiserum resulted in a same pattern of dose-related inhibition of (Na(+)+K+)-ATPase activity in control and ethanol-fed rats, whereas Mg(2+)-ATPase was not affected by the antiserum. These results suggest that the mechanism of ethanol-induced enhancement of renal (Na(+)+K+)-ATPase activity could be explained through an increase in the number of catalytic units.
Rodrigo
R
R
Department of Experimental Medicine, University of Chile, Santiago.
Novoa
E
E
Thielemann
L
L
Granata
P
P
Videla
L
L
D000704:Analysis of Variance; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000302:isolation & purification; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D007106:Immune Sera; D007074:Immunoglobulin G; D000431:Ethanol; D012964:Sodium; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium