Tissue-specific molecular heterogeneity of human growth hormone-releasing hormone receptor protein.
A site-directed anti-peptide antibody (anti-hGHRHRc18) was generated against the cytoplasmic tail of human GHRH receptor. The dissociation constant (Kd) and the antibody binding site (AbT) of anti-hGHRHRc18 were 2.5 nmol/l and 0.54 nmol/l, respectively. In an immunoblotting experiment, affinity-purified anti-hGHRHRc18 specifically recognized a single 50-kDa protein in human pituitary. In a screening of the expression of GHRH receptor protein in extra-pituitary tissues, only human kidney showed a single 52-kDa protein. Our results suggest that the GHRH receptor protein exhibits tissue-specific molecular heterogeneity.
Fujinaka
Y
Y
First Department of Internal Medicine, The University of Tokushima, Japan.
Yokogoshi
Y
Y
Zhang
C Y
CY
Okura
T
T
Kitagawa
K
K
Saito
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D018013:Receptors, Neuropeptide / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology
D000906:Antibodies; D018013:Receptors, Neuropeptide; D018017:Receptors, Pituitary Hormone-Regulating Hormone; C048250:somatotropin releasing hormone receptor