title: Direct effects of 3,5,3'-triiodothyronine and 3,5-diiodothyronine on mitochondrial metabolism in the goldfish Carassius auratus.
abstract: Direct effects of 3,5,3'-triiodothyronine (T3) and 3,5-diiodothyronine (T2) on the metabolism of the goldfish (Carassius auratus) were assessed using mitochondria isolated from liver and red muscle. Following a 5-min incubation with either T3 or T2, the oxidation rates of substrates involved in amino acid and carbohydrate metabolism and lipid catabolism were measured. State 3 oxidation of pyruvate was significantly higher for liver mitochondria treated with T2 and for red muscle mitochondria incubated with T3 when compared to control mitochondria. Rapid elevation of state 3 rates of substrate oxidation by thyroid hormones may be important in mediating diurnal changes in mitochondrial metabolism. Significant increases in liver and red muscle mitochondrial state 4 rates were also observed for pyruvate in T2- and T3-treated mitochondria and for glutamate in T3-treated mitochondria.
authors.affiliation: Department of Zoology, University of Guelph, Ontario, Canada.
authors.forename: S C
authors.initials: SC
authors.lastname: Leary
authors.forename: K N
authors.initials: KN
authors.lastname: Barton
authors.forename: J S
authors.initials: JS
authors.lastname: Ballantyne
mesh_terms: D000818:Animals; D004104:Diiodothyronines / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D010172:Palmitoylcarnitine / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D014285:Triiodothyronine, Reverse / Q000494:pharmacology*
chemical_list: D004104:Diiodothyronines; D010172:Palmitoylcarnitine; D018698:Glutamic Acid; C030103:3,5-diiodothyronine; D014285:Triiodothyronine, Reverse; D019289:Pyruvic Acid