Design and characterisation of long-R3-insulin-like growth factor-I muteins which show resistance to pepsin digestion.
Site-directed mutagenesis was used to construct pepsin-resistant, single-point mutations of the N-terminal extended IGF-I analogue, long-R3-IGF-I. In order to identify the most susceptible sites, the kinetics of long-R3-IGF-I digestion by purified porcine pepsin were determined. Pepsin initially cleaved the Leu10-Phe11 bond in the N-terminal extension peptide to generate FVN-R3-IGF-I, followed in rapid succession by cleavage at Gln15-Phe16, Tyr24-Phe25, Leu10-Val11 and Met59-Tyr60 in the IGF-I moiety. Single-point mutations at these sites were designed on the basis of the preferred cleavage bonds for pepsin, as well as amino acid substitutions less likely to disturb protein structure. These included Leu10Val, Phe16Ala, Phe25Leu, Asp53Glu and Met59Gln. All five muteins retained growth-promoting activity equivalent to or higher than that of IGF-I. In terms of pepsin susceptibility, Leu10Val and Asp53Glu were degraded as rapidly as the parent long-R3-IGF-I, Met59Gln and Phe25Leu were partially stabilised, and Phe16Ala showed a marked improvement in stability over a wide range of pepsin:substrate ratios. Accordingly, the Phe16Ala mutein, long-R3A16-IGF-I, has potential for oral applications to enhance gastric growth and repair.
Bryant
K J
KJ
Cooperative Research Centre for Tissue Growth and Repair: Department of Biochemistry, University of Adelaide, Australia.
Read
L C
LC
Forsberg
G
G
Wallace
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D002479:Inclusion Bodies / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010434:Pepsin A / Q000378:metabolism*; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D013552:Swine
C085700:long R(3)-insulin-like growth factor-I; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1; D010434:Pepsin A