title: Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).
abstract: The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.
authors.affiliation: Abteilung für Innere Medizin m.S. Hämatologie und Onkologie, Virchow Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Nagel
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: C
authors.initials: C
authors.lastname: Thiede
authors.forename: D
authors.initials: D
authors.lastname: Huhn
authors.forename: A
authors.initials: A
authors.lastname: Neubauer
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016313:Genes, abl / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000235:genetics; D007372:Interferons / Q000627:therapeutic use; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007372:Interferons; D011163:Hydroxymethylbilane Synthase