Stabilization of chlorophyll a-binding apoproteins P700, CP47, CP43, D2, and D1 by chlorophyll a or Zn-pheophytin a.
Stabilization of chlorophyll a-binding apoproteins P700, CP47, CP43, D2, and D1 against proteolytic degradation has been investigated through in vitro synthesis of chlorophyll a or Zn-pheophytin a in intact etioplasts from barley. Stabilization of the apoproteins was dependent on the concentration of chlorophyll a or Zn-pheophytin a. Zn-pheophytin a was superior to chlorophyll a with respect to the concentration of pigment required for an equal yield of the stabilized chlorophyll a protein CP47, CP43, and P700 and for the total yield of chlorophyll a proteins. Zn-pheophytin a was most efficient for stabilizing CP47 and, at an increased concentration, efficient for stabilizing CP43, P700, and D1. Stabilization of apoproteins was highest after de novo synthesis of 90-300 pmol of Zn-pheophytin a or of about 400-600 pmol of chlorophyll a/4.2 x 10(7) etioplasts. The yield of stabilized chlorophyll proteins decreased at higher concentrations of Zn-pheophytin a, but was unaffected by higher concentrations of chlorophyll a.
Eichacker
L A
LA
Department of Botany, University of Munich, 80638 München, Menzinger Strasse 67, Federal Republic of Germany. lutz@botanik.biologie.uni-muenchen.de
Helfrich
M
M
Rüdiger
W
W
Müller
B
B
D001059:Apoproteins / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000077194:Chlorophyll A; D045342:Light-Harvesting Protein Complexes; D008274:Magnesium / Q000378:metabolism; D010674:Pheophytins / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D015032:Zinc / Q000378:metabolism
D001059:Apoproteins; D045342:Light-Harvesting Protein Complexes; D010674:Pheophytins; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C070970:photosystem II, chlorophyll-binding protein, CP-47; D002734:Chlorophyll; C001785:chlorophyll P 700; C061694:pheophytin a; D008274:Magnesium; D015032:Zinc; D000077194:Chlorophyll A