Characterisation of the novel junctions of two minichromosomes of Leishmania major.
The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly repeated 3 times, which was also repeated elsewhere in the genome. DNA fragments containing the novel junctions of the minichromosomes M210 and M230 have been likewise cloned. The nucleotide sequence of these fragments was determined and compared with their corresponding DNAs in the source chromosome. It revealed that M210 contains a central 226 bp non-repeated fragment, whereas the central fragment of M230 was 5 kb long. A 9 bp sequence which was repeated in inverted orientation around the recombination points of M230 was found. M210 showed instead an A + T rich DNA which could be implicated in its generation.
Ortiz
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G
Departamento de Genética y Microbiología, Facultad de Medicina, Universidad de Murcia, Spain.
Segovia
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M
D000818:Animals; D001483:Base Sequence; D007446:Chromosome Inversion; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics*; D018503:Genome, Protozoan; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan