Physical and functional mapping of the transcriptional start sites of Plasmodium falciparum proliferating cell nuclear antigen.
RNase protection assays and primer extension analysis have been used to locate a major transcription start site 960 bp upstream from the translational start of the PfPCNA coding sequence. A second, minor, site is situated a further 40 bp upstream. Intraerythrocytic parasite stages were transiently transfected with constructs containing a firefly luciferase reporter gene under the transcriptional control of variously modified elements of the PfPCNA 5' flanking sequence. These experiments identified a 470 bp region essential for promoter activity, which contains the physically mapped transcriptional start sites. In addition, a region between 290 and 620 bp upstream of the transcriptional start sites is required for efficient promoter activity.
Horrocks
P
P
Zentrum für Infektionsforschung, Universität Würzburg, Germany.
Kilbey
B J
BJ
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D016054:DNA, Protozoan; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D016054:DNA, Protozoan; D004274:DNA, Recombinant; D018809:Proliferating Cell Nuclear Antigen; D015800:Protozoan Proteins