Control of ornithine decarboxylase activity by polyamines and absence of antizyme in Tetrahymena.
1. In cells of Tetrahymena pyriformis and thermophila, ODC activity was significantly suppressed but ODC decay was not stimulated by putrescine. 2. Free antizyme and ODC-antizyme complex were both not detected in extracts of cells of T. pyriformis treated with putrescine. 3. It was concluded that in Tetrahymena, unlike vertebrate cells, ODC is not subject to polyamine-induced destabilization mediated by antizyme.
Koguchi
K
K
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Murakami
Y
Y
Hayashi
S
S
D000818:Animals; D015317:Biogenic Polyamines / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000187:drug effects*; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins / Q000494:pharmacology; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013768:Tetrahymena / Q000187:drug effects* / Q000201:enzymology
D015317:Biogenic Polyamines; D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins; C031017:ornithine decarboxylase antizyme; D013096:Spermine; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine