Reactivation of herpes simplex virus type 1 in cells of latently infected mouse trigeminal ganglia cultured in serum-free medium.
Herpes simplex virus type 1 (HSV-1) was reactivated more rapidly in cells of latently infected mouse trigeminal ganglia which were cultured in serum-free medium (after 3.7 days of cultivation) than in those cultured in serum-containing Dulbecco's modified Eagle's medium (after 8.5 days of cultivation). The concentration of calcium ion (Ca2+) in the medium affected HSV-1 reactivation in ganglionic cultures, and 0.9 mM was the optimum concentration for the reactivation. Reactivation was delayed significantly in ganglia put into culture 4 months or more after infection compared with those cultured 1 month after infection.
Kimura
S
S
Department of Microbiology, Kochi Medical School, Japan.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006561:Herpes Simplex; D007700:Kinetics; D051379:Mice; D018139:Simplexvirus / Q000254:growth & development*; D012668:Trigeminal Ganglion / Q000821:virology*; D017735:Virus Latency
D016895:Culture Media, Serum-Free; D002118:Calcium