Detection of rabies virus antigen in animals by avidin-biotin dot ELISA.
An avidin-biotin dot ELISA test was standardized to detect rabies viral antigens from the brain of rabies-suspected animals. This test was compared with the direct fluorescent antibody test (FAT). The advantages of the avidin-biotin dot ELISA are discussed. The incorporation of avidin-biotin into a conventional ELISA is a step forward in improving the available rabies antigen detection procedures as this technique is more sensitive, highly specific and exploits the great affinity of avidin for biotin. No significant difference was observed between FAT and avidin-biotin dot ELISA.
Jayakumar
R
R
Department of Animal Biotechnology, Madras Veterinary College, India.
Thirumurugan
G
G
Nachimuthu
K
K
Padmanaban
V D
VD
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002415:Cats; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct; D006041:Goats; D011818:Rabies / Q000821:virology*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D012756:Sheep
D000956:Antigens, Viral; D001360:Avidin; D001710:Biotin