C1q is a nucleotide binding protein and is responsible for the ability of clusterin preparations to promote immune complex formation.
Clusterin prepared from human serum by monoclonal antibody affinity chromatography was devoid of the ability to increase the rates of formation of insoluble immune complexes associated with clusterin preparations obtained by polyclonal IgG affinity chromatography. Clusterin did not bind to AMP-Sepharose but the protein responsible for increasing the rates of formation of insoluble immune complexes did bind to this affinity matrix. This protein was identified as complement protein C1q on the basis of its behaviour on SDS/PAGE and reactivity in sandwich ELISA with monoclonal antibodies specific for C1q. C1q (identified from its behaviour on SDS/PAGE, immunoreactivity with C1q-specific monoclonal antibodies and N-terminal sequencing data) was purified from serum by AMP-Sepharose chromatography. The binding of C1q to AMP-Sepharose was inhibited by adenine nucleotides.
Roeth
P J
PJ
Department of Biochemistry, University of Sydney, NSW, Australia.
Easterbrook-Smith
S B
SB
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D051152:Clusterin; D015922:Complement C1q / Q000378:metabolism*; D003169:Complement Inactivator Proteins / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018832:Molecular Chaperones; D011485:Protein Binding; D017421:Sequence Analysis
D000227:Adenine Nucleotides; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D007136:Immunoglobulins; D018832:Molecular Chaperones; D000249:Adenosine Monophosphate; D015922:Complement C1q