Nucleotide sequence of the Bacillus coagulans homologue of the spoIIA operon of Bacillus subtilis.
The spoIIA locus of Bacillus coagulans (Bc) was cloned into pTZ18R and the nucleotide sequence was determined. To clone the operon, one PCR primer corresponding to the C-terminal region of SpoIIAB, and a second corresponding to a region near the middle of SpoIIAC, were designed on the basis of the three previously published Bacillus spoIIA sequences. The Bc spoIIA sequence contains three open reading frames coding for putative proteins of 116, 147 and 252 aa.
Park
S G
SG
Department of Biochemistry, University of Oxford, UK.
Yudkin
M D
MD
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D012808:Sigma Factor; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus