Leukosialin (CD43)-major histocompatibility class I molecule interactions involved in spontaneous T cell conjugate formation.
Resting T cells spontaneously adhere in a selective manner to potent accessory cells, such as dendritic cells (DC) and lymphoblastoid B blasts (LCL). Here we demonstrate that leukosialin (CD43) and major histocompatibility complex class I molecules (MHC-I) might play a critical role in this process. T cell conjugate formation with monocyte-derived DC (md-DC) and LCL could be strongly inhibited by either preincubating T cells with Fab fragments of CD43 monoclonal antibody (mAb) 6F5 or by preincubating md-DC or LCL with MHC-I mAb W6/32. Intact CD43 mAb 6F5, in contrast to monovalent Fab fragments, enhanced T cell adhesiveness by transactivating CD2 binding to CD58 molecules. Interestingly, induction of this proadhesive signal via CD43 with intact 6F5 mAb was found to revert mAb W6/32-mediated inhibition of T cell conjugate formation. These observations indicated that CD43 cross-linkage mimics and monovalent mAb 6F5 inhibits interaction of T cell CD43 with a stimulatory ligand on opposing cells, presumably MHC-I. For the demonstration of direct physical interaction between CD43 on T cells and MHC-I-coated beads it was necessary, however, to ligate CD2 on T cells with a stimulatory pair of CD2 mAbs (VIT13 plus TS2/18). This suggests that CD2 ligation crosswise upregulates CD43 binding avidity for MHC-I and that both adhesion molecule pairs (CD43/MHC-I and CD2/CD58) act in concert to induce and mediate T cell conjugate formation with certain cell types.
Stöckl
J
J
Institute of Immunology, University of Vienna, Austria.
Majdic
O
O
Kohl
P
P
Pickl
W F
WF
Menzel
J E
JE
Knapp
W
W
D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000276:immunology; D018801:CD2 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D051917:Leukosialin; D008285:Major Histocompatibility Complex / Q000276:immunology*; D012795:Sialoglycoproteins / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D015533:Transcriptional Activation
D015703:Antigens, CD; D018801:CD2 Antigens; D015395:Histocompatibility Antigens Class I; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins