title: Induction of apoptotic cell death in the seminiferous tubule of the adult rat testis: assessment of the germ cell types that exhibit the ability to enter apoptosis after hormone suppression by oestradiol treatment.
abstract: The involvement of apoptosis in germ cell degeneration in the adult rat testis after gonadotrophin suppression has recently been shown in several studies, which have focused on the localization of apoptotic cells to the stages of the spermatogenic cycle. However, the precise germ cell types and maturing steps at which apoptosis is elicited remain controversial. The present study used oestradiol treatment to produce hormone suppression and to study induced germ cell degeneration. Adult male rats were administered a daily injection of 50 micrograms oestradiol benzoate for 5, 10 or 15 days. Characterization of the ultrastructural features of the dying cells and in-situ 3'-end labelling of DNA showed clearly that the deaths of all the germ cell types occurred by apoptosis. High-resolution light microscopy, although time-consuming, resulted in a precise method for analysis of the localization of the cells involved. Stages IV-X of the seminiferous epithelium were found to be the most sensitive to degeneration in response to oestradiol treatment. Our results are discussed in the light of current knowledge about the hormonal control of the spermatogenic cycle. Oestradiol treatment has proved to provide a suitable in-vivo model to study germ cell death.
authors.affiliation: Departmento de Biología Celular, Facultad de Medicina, Vallodolid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Blanco-Rodríguez
authors.forename: C
authors.initials: C
authors.lastname: Martínez-García
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053938:DNA Fragmentation; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: C074283:estradiol 3-benzoate; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone