Alzheimer-associated presenilin-2 confers increased sensitivity to apoptosis in PC12 cells.
Presenilin-2 is a gene of unknown function recently identified based upon linkage with some forms of familial Alzheimer's disease. To investigate potential effects of PS-2 on cell viability, rat pheochromocytoma (PC12) cells were stably transfected with cDNA constructs encoding either full-length human PS-2 or, for comparison, mouse Bcl-X(L). Overexpression of PS-2 conferred increased sensitivity to the apoptotic stimuli staurosporine and hydrogen peroxide. In contrast, Bcl-X(L) overexpression significantly reduced cell death induced by these stimuli. These results suggest that one function of PS-2 may involve modulation of cell viability.
Deng
G
G
Institute for Brain Aging and Dementia, University of California, Irvine 92697-4540, USA. gdeng@parker.bio.uci.edu
Pike
C J
CJ
Cotman
C W
CW
D000544:Alzheimer Disease; D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D016716:PC12 Cells; D053766:Presenilin-2; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D019311:Staurosporine / Q000494:pharmacology; D014162:Transfection; D051020:bcl-X Protein
C494811:BCL2L1 protein, human; C494812:Bcl2l1 protein, mouse; C494813:Bcl2l1 protein, rat; D008565:Membrane Proteins; C508861:PSEN2 protein, human; D053766:Presenilin-2; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D006861:Hydrogen Peroxide; D019311:Staurosporine