title: Probing the structure and mobility of Pseudomonas aeruginosa azurin by circular dichroism and dynamic fluorescence anisotropy.
abstract: The UV dynamic fluorescence and CD of several Pseudomonas aeruginosa azurins bearing single amino acid mutation have been studied. Two classes of mutants were examined. In the first class, two hydrophobic residues in the core of the protein, Ile 7 and Phe 110, nearest to the azurin single tryptophan Trp 48, were substituted by a serine (mutants 17S and F110S). In the second class, two residues in the outer sphere of the copper ligand field were changed, obtaining the following mutants: M44K, H35F, H35L, and H35Q. All these proteins showed two fluorescence lifetimes in the copper-containing form, but only one in the copper-free form. The lifetime of the latter derivatives was different from either those of the metal-bound samples, definitely ruling out the presence of apo-like species in the holo protein. Copper-free 17S and F110S showed a more complex fluorescence decay profile requiring a distribution of lifetimes rather than a single lifetime. Holo F110S was also better fitted, in the limit of confidence, with two distributions rather than a pair of lifetimes. Time-resolved anisotropy of these two mutants as well as of wild-type (wt) protein showed two components (rotational times for wt < or = 200 ps and 7 ns, respectively). These components were not affected significantly by copper removal in the case of wt protein. Instead, the short rotational component of the mutants dropped dramatically to values near zero, indicating a much greater mobility of the tryptophanyl residue in the mutant apo azurins. These data were supported by CD measurements showing a small effect of the copper presence in the region below 250 nm, i.e., in the secondary structure, but almost a collapse of the aromatic asymmetry at 270-295 nm related to a relaxation of the structural constraint around the tryptophan. Altogether these data show that copper does not play a structural role in wt azurin, whereas it is crucial in the stabilization of 17S and F110S mutants. Furthermore, although the metal site geometry is rigidly kept in wt apo-azurin, it regains the native form only in the presence of the metal in the "core" mutants. This finding is important for the theory of entatic states in metalloproteins (Williams RJP, 1995, Eur J Biochem 234:363-381).
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universitá di Roma Tor Vergata, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mei
authors.forename: G
authors.initials: G
authors.lastname: Gilardi
authors.forename: M
authors.initials: M
authors.lastname: Venanzi
authors.forename: N
authors.initials: N
authors.lastname: Rosato
authors.forename: G W
authors.initials: GW
authors.lastname: Canters
authors.forename: A F
authors.initials: AF
authors.lastname: Agró
mesh_terms: D001400:Azurin / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D005454:Fluorescence Polarization; D016297:Mutagenesis, Site-Directed; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D001400:Azurin