Fluoro-Green and Fluoro-Red: two new fluorescent retrograde tracers with a number of unique properties.
As a means of improving nerve tract-tracing in the peripheral and central nervous systems we experimented with two (retrograde) fluorescent emulsions, which we have tentatively named Fluoro-Green (FGr) and Fluoro-Red (FRe), and which we believe possess the following seven advantages: (1) they show little diffusion beyond the injection site; (2) their excitation/emission characteristics allow their use in double-tracing experiments; (3) they do not 'leak' from labeled cells; (4) their fluorescence is presented as large granules in the cytoplasm and its processes; (5) the fluorescence lasts for a sufficiently long time to permit repeated observation; (6) they may be used in combination with a wide variety of other neuroanatomical tracing methods; (7) they are economical, non-toxic and easy to utilize.
Dong
K
K
First Department of Anatomy, Kobe University School of Medicine, Japan.
Qu
T
T
Ahmed
F A
FA
Zhang
L
L
Yamada
K
K
Guison
N G
NG
Miller
M
M
Yamadori
T
T
D000818:Animals; D001370:Axonal Transport; D001562:Benzimidazoles / Q000633:toxicity; D001565:Benzoates / Q000633:toxicity; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D004655:Emulsions; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
D001562:Benzimidazoles; D001565:Benzoates; D004655:Emulsions; D005456:Fluorescent Dyes; C104021:Fluoro-Green; C104022:Fluoro-Red