A rapid genotype assay for Pla haplotypes.
The method described here results in simple visual analysis of 1, 2, or 3 ethidium bromide-stained bands corresponding to the PlA1, PlA2 or PlA1/A2 phenotypes, respectively. The amplification primers, designed to yield a 711bp product which encompasses exon 2 and substantial intronic sequence both 5' and 3' of the exon enable exquisite resolution of the restriction enzyme digestion products which define the alleles. In addition to prediction of fetal risk of neonatal alloimmune thrombocytopenia using this method, an ethnically heterogeneous pool of platelet donors was screened for the platelet antigen PlA (HPA-1) and gave unequivocal results which should be useful for platelet matching.
Pfaffenbach
G M
GM
Laboratory of Immunogenetics, New York Blood Center, New York 10021, USA.
Bussel
J A
JA
Rubinstein
P
P
D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D004396:Coloring Agents; D004247:DNA / Q000235:genetics*; D004996:Ethidium; D005260:Female; D015894:Genome, Human; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011296:Prenatal Diagnosis / Q000379:methods*; D013698:Templates, Genetic; D013921:Thrombocytopenia / Q000175:diagnosis*; D013997:Time Factors
D016824:Antigens, Human Platelet; D004396:Coloring Agents; D004247:DNA; D004996:Ethidium