title: Intracellular glutathione as a possible direct blocker of HIV type 1 reverse transcription.
abstract: In AIDS patients, chronic inflammation and elevated levels of cytokines seem to be associated with reduced levels of glutathione (GSH). GSH has been proposed to inhibit the activation of NF-kB, which results in the inhibition of HIV-1 replication. Here, we show the evidence that GSH and N-acetylcysteine, but not L-cysteine or dithiothreitol, could inhibit the reverse transcriptase (RT) process of HIV-1. Such inhibition was not observed with the RT of murine leukemia virus.
authors.affiliation: Section of Serology, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kameoka
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Tobiume
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D001224:Aspartic Acid / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D006801:Humans; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D004279:DNA, Viral; D001224:Aspartic Acid; D054303:HIV Reverse Transcriptase; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol; D000111:Acetylcysteine