title: Rapid determination of amphetamine stereoisomer ratios in urine by gas chromatography-mass spectroscopy.
abstract: We describe a method for the determination of amphetamine optical isomer ratios. A simple basic extraction of amphetamine from urine is followed by on-column derivatization with (S)-(-)-N-(trifluoroacetyl)-L-prolyl chloride and analysis by gas chromatography-mass spectroscopy. This approach has been shown to be satisfactory for the separation of d- and l-amphetamine isomers and precision data shows the method to be reproducible. Patient data is presented to demonstrate the ability to differentiate between patients taking d-amphetamine and those using illicit (racemic) amphetamine by their l/d amphetamine ratio.
authors.affiliation: Department of Clinical Biochemistry, Salford Royal Hospitals NHS Trust, UK.
authors.forename: V A
authors.initials: VA
authors.lastname: Tetlow
authors.forename: J
authors.initials: J
authors.lastname: Merrill
mesh_terms: D000661:Amphetamine / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D000661:Amphetamine