[AP-PCR and AP-PCR-SSCP analyses].
DNA fingerprinting by arbitrarily primed polymerase chain reaction (AP-PCR) is a simple and informative method to investigate genetic changes in cancers. The discovery of a mutator phenotype in hereditary non-polypotic colon cancer by the AP-PCR fingerprinting demonstrated the usefulness of this unbiased method. Due to its semiquantitative amplification of the target DNA, the AP-PCR fingerprinting can be applied to analyze numerical changes of chromosome fragments. A recent breakthrough of the chromosomal assignment of human AP-PCR products enabled us to do PCR-mediated molecular karyotyping of cancers. Application of single-strand conformation polymorphism (SSCP) analysis to AP-PCR products seems to be promising to elucidate somatic point mutations and hidden losses of heterozygosity in the anonymous regions in cancers.
Yasuda
J
J
Oncogene Division, National Cancer Center Research Institute.
Okano
A
A
Sekiya
T
T
D003110:Colonic Neoplasms / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D018740:Genetic Heterogeneity; D006801:Humans; D007621:Karyotyping; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm