title: Absence of cross-reactivity of IgE antibodies from subjects allergic to Hevea brasiliensis latex with a new source of natural rubber latex from guayule (Parthenium argentatum).
abstract: BACKGROUND
  Natural rubber latex sensitization is caused by proteins from the latex of Hevea brasiliensis, the Brazilian rubber tree. Alternative non-cross-reactive latex rubber sources are needed for the manufacture of nonallergenic medical devices (e.g., catheters) that require elasticity, which is not currently provided by synthetic materials.
  
  
  METHODS
  We have studied the ability of IgE antibodies from subjects allergic to Hevea latex and IgG antibodies from hyperimmunized mice to detect proteins derived from guayule (Parthenium argentatum), an alternative rubber source, using a direct binding and competitive inhibition RAST and Western blot analysis.
  
  
  RESULTS
  IgE antibodies from 62 subjects allergic to Hevea latex (46 adults and 16 children with spina bifida) and from serum pools of adults allergic to Hevea latex (n = 183), pediatric patients (n = 101), and patients with spina bifida (n = 53), as well as IgG antibodies from hyperimmunized mice, were unable to detect any proteins in guayule by RAST or Western blot analysis. No competitive inhibition of IgE anti-Hevea binding to Hevea solid phase was detected by the preincubation of sera from subjects allergic to Hevea latex with soluble guayule latex before RAST analysis.
  
  
  CONCLUSIONS
  These results demonstrate that proteins in guayule latex are not cross-reactive with Hevea latex allergens. Devices that require the elasticity provided by rubber may be manufactured with guayule latex as an alternative rubber source for patients with Hevea latex allergy.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Siler
authors.forename: K
authors.initials: K
authors.lastname: Cornish
authors.forename: R G
authors.initials: RG
authors.lastname: Hamilton
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000641:Ammonia; D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002404:Catheterization; D002648:Child; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology*; D004342:Drug Hypersensitivity / Q000175:diagnosis / Q000209:etiology*; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007840:Latex / Q000276:immunology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D016273:Occupational Exposure; D010940:Plant Proteins; D011145:Polyvinyls; D011852:Radioallergosorbent Test; D012408:Rubber / Q000009:adverse effects*; D013045:Species Specificity; D016135:Spinal Dysraphism / Q000150:complications; D005465:Fluorocarbon Polymers
chemical_list: D000485:Allergens; D000641:Ammonia; D003577:Cytochrome P-450 Enzyme System; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D007840:Latex; D010940:Plant Proteins; D011145:Polyvinyls; D012408:Rubber; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C093121:RPP30 protein, Parthenium argentatum