Functional characterization by heterologous expression of a novel cloned tachykinin peptide receptor.
An orphan receptor resembling the neurokinin 3 tachykinin receptor (NK3), initially claimed to be an atypical opioid receptor, is shown herein to respond potently to the physiological NK3 receptor ligand, neurokinin B. This 'NK4' receptor did not give functional responses in Xenopus oocytes to opioid agonists. However, NK4 receptor activation was inhibited by nanomolar concentrations of dynorphin. The NK4 receptor is therefore a tachykinin receptor which is functionally antagonized by an endogenous opioid peptide.
Donaldson
L F
LF
Department of Biological Chemistry, University of California at Davis Medical School 95616, USA.
Haskell
C A
CA
Hanley
M R
MR
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004399:Dynorphins / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D015287:Neurokinin B / Q000494:pharmacology; D009865:Oocytes / Q000502:physiology; D051381:Rats; D017473:Receptors, Opioid, kappa / Q000819:agonists; D018039:Receptors, Tachykinin / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
D018076:DNA, Complementary; D017473:Receptors, Opioid, kappa; D018039:Receptors, Tachykinin; C102505:neurokinin receptor 4; D004399:Dynorphins; D015287:Neurokinin B