Transforming growth factor-beta1 inhibits human keratinocyte proliferation by upregulation of a receptor-type tyrosine phosphatase R-PTP-kappa gene expression.
We studied regulation of a receptor-type tyrosine phosphatase R-PTP-kappa gene expression in a human keratinocyte cell line, HaCaT. Addition of TGF-beta 1 to the HaCaT cells markedly induced the expression of R-PTP-kappa mRNA in a time- and dose-dependent manner. The induction of R-PTP-kappa mRNA expression was observed at a dose as low as 0.02 ng/ml TGF-beta1 and reached a peak at 2 ng/ml TGF-beta 1 after 6 h treatment. The TGF-beta 1-induced R-PTP-kappa mRNA expression was suppressed by sodium orthovanadate, a tyrosine phosphatase inhibitor, and H7, a serine/threonine kinase inhibitor, but not by genistein, a tyrosine kinase inhibitor. In addition, the inducing effect is not dependent on de novo protein synthesis. Taken together, these results suggest that TGF-beta 1 inhibits the human keratinocyte proliferation in vitro, possibly through induction of R-PTP-kappa gene expression.
Yang
Y
Y
Department of Biological Sciences, KAIST, Seoul National University, Suwon, Republic of Korea.
Gil
M
M
Byun
S M
SM
Choi
I
I
Pyun
K H
KH
Ha
H
H
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015854:Up-Regulation; D014638:Vanadates / Q000494:pharmacology
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014638:Vanadates; C489852:PTPRK protein, human; D017027:Protein Tyrosine Phosphatases; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2