High-performance liquid chromatography of long chain aliphatic aldehydes with peroxyoxalate chemiluminescence detection utilizing a fluorogenic reagent, 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole.
A peroxyoxalate chemiluminescence detection in high-performance liquid chromatography was examined for determination of long chain aliphatic aldehydes using 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H) as a fluorogenic labelling reagent. A mixture of 0.6 mM bis(2,4,6-trichlorophenyl)oxalate and 100 mM H2O2 in acetonitrile was used as a post-column chemiluminescent reagent. DBD-hydrazones of five long chain aliphatic aldehydes (C11-C18:1) were eluted and well separated within 16 min with a mobile phase of 5 mM imidazole in acetonitrile. The detection limits were 14-18 fmol at a signal-to-noise ratio (S/N) of 2. DBD-hydrazones of four aliphatic aldehydes (C6-C11) were also eluted and well separated within 30 min with a mobile phase of 5mM imidazole buffer (pH 7.5)-acetonitrile (20/80, v/v). The detection limits were 86-152 fmol (S/N = 2).
Nakashima
K
K
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Yoshida
T
T
Kuroda
N
N
Akiyama
S
S
D000447:Aldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006835:Hydrazones / Q000737:chemistry; D008163:Luminescent Measurements; D010069:Oxadiazoles; D010070:Oxalates / Q000737:chemistry; D013449:Sulfonamides
D000447:Aldehydes; D005456:Fluorescent Dyes; D006835:Hydrazones; D010069:Oxadiazoles; D010070:Oxalates; D013449:Sulfonamides; C080612:4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole; C044622:peroxyoxalate