Phenotypic and genotypic characterization of clinically recovered presumptive Acinetobacter baumannii isolates which failed to grow at 44 degrees C.
Eleven clinical isolates of Acinetobacter, which exhibited an identical biochemical profile compatible with genospecies 3 and failed to grow at 44 degrees C, were not agglutinated by polyclonal rabbit immune sera against 26 serovars of genospecies 3. Rather, all 11 isolates reacted strongly with antiserum against serovar 18 of A. baumannii. Macrorestriction (SmaI) analysis of genomic DNA revealed that only one isolate was genotypically different, whereas the remaining ones were either closely related or identical. However, the genomic DNA of the A. baumannii serovar 18 reference strain proved to be genotypically unrelated.
Traub
W H
WH
Institut für Medizinische Mikrobiologie, Universität des Saarlandes, Homburg/Saar, Germany.
Bauer
D
D
Leonhard
B
B
D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D010641:Phenotype; D011817:Rabbits; D013696:Temperature
D004269:DNA, Bacterial