Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro.
To investigate the utility of primary cultures of bovine hepatocytes for compartmentalized acute phase protein studies the secretion of serum amyloid-A (SAA) and haptoglobin (Hp) was measured after stimulation by pro-inflammatory cytokines (recombinant human IL-6 (rhIL-6) and recombinant human tumour necrosis factor-alpha (rhTNF-alpha)). During the incubation period of the experiment, the SAA and Hp secretion into the culture medium increased (P < 0.05). SAA concentrations showed an additional increase following treatment with each of the cytokines (P < 0.01). Hp concentrations remained unchanged, whereas incubation with a combination of both resulted in a significant increase of the medium concentration of both SAA (P < 0.01) and Hp (P < 0.05). From these findings it is concluded that primary bovine hepatocytes can be used for in vitro studies on acute-phase protein secretion.
Alsemgeest
S P
SP
Department of Pathology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
van 't Klooster
G A
GA
van Miert
A S
AS
Hulskamp-Koch
C K
CK
Gruys
E
E
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D000685:Serum Amyloid A Protein / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000209:Acute-Phase Proteins; D001053:Apolipoproteins; D016207:Cytokines; D006242:Haptoglobins; D015850:Interleukin-6; D011498:Protein Precursors; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D014409:Tumor Necrosis Factor-alpha