Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene.
A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.
Kobayashi
T
T
Department of Molecular Immunology, Kyushu University, Fukuoka, Japan.
Inoue
I
I
Jenkins
N A
NA
Gilbert
D J
DJ
Copeland
N G
NG
Watanabe
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004285:Dogs; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011970:Receptors, Histamine H2 / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D012333:RNA, Messenger; D011970:Receptors, Histamine H2