Tissue-specific self-peptides bound by major histocompatibility complex class I molecules of a human pancreatic beta-cell line.
The process of beta-cell destruction in IDDM is mediated, in part, by CD8+ T-cells. Structural characterization of HLA-I-bound self-peptides presented by the human beta-cell line HP-62 was performed to identify possible tissue-specific autoantigens in the context of CD8+ T-cell/HLA-I interactions. The sequences of the beta-cell line HLA-I-bound peptides were compared with sequence databases. Six of the obtained sequences showed homology to known precursor proteins, three of which--GLUT2 receptor, phosphatidylinositol-glycan-specific phospholipase D, and 5-hydroxytryptamine-1F receptor--have a limited, tissue-specific expression. These HLA-bound self-peptides may be part of a pool of autoantigens recognized by beta-cell reactive cytotoxic T-cells.
Papadopoulos
K P
KP
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Colovai
A I
AI
Maffei
A
A
Jaraquemada
D
D
Suciu-Foca
N
N
Harris
P E
PE
D000595:Amino Acid Sequence; D001324:Autoantigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D051273:Glucose Transporter Type 2; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D010739:Phospholipase D / Q000737:chemistry / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology; D011985:Receptors, Serotonin / Q000737:chemistry / Q000276:immunology
D001324:Autoantigens; D051273:Glucose Transporter Type 2; D015395:Histocompatibility Antigens Class I; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D011985:Receptors, Serotonin; D010739:Phospholipase D; C065017:glycoprotein phospholipase D