Isolation and characterization of a functional promoter from Nitrosomonas europaea.
A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at -35 and -10 have low similarity to the -10/-35 consensus sequence of known prokaryotic promoters.
Nejidat
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Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Israel.
Spector
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Abeliovich
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D000641:Ammonia / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009606:Nitrosomonas / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial; D000641:Ammonia