title: Buffalo (Bos buffali L.) chymosin purification and properties.
abstract: Buffalo chymosin was isolated from abomasum mucosa extract of buffalo calves by affinity chromatography on gramicidin S-agarose followed by ion exchange chromatography on gamma-aminopropylsilochrom. Its molecular weight, 36 +/- 1 kDa, is similar to that of bovine calf chymosin. The N-terminal sequence Gly-Glu-Val-Ala-Ser-Val-Pro- coincides with that of bovine enzyme, whereas some differences were found in the amino acid composition of these enzymes. Buffalo and bovine enzyme possess similar but not identical structures. General proteolytic and milk-clotting activities of buffalo chymosin are also similar to those of bovine proteinase. pH-Optimum of its activity against hemoglobin lies at pH 4.0, somewhat higher than that for bovine chymosin, which indicates subtle differences in the functional properties of two enzymes.
authors.affiliation: Chemistry Department, Mansoura University, Egypt.
authors.forename: C A
authors.initials: CA
authors.lastname: Abdel Malak
authors.forename: I F
authors.initials: IF
authors.lastname: Abou El Adab
authors.forename: V
authors.initials: V
authors.lastname: Vukashinovic
authors.forename: I A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: E A
authors.initials: EA
authors.lastname: Timokhina
authors.forename: G I
authors.initials: GI
authors.lastname: Lavrenova
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D002417:Cattle; D012085:Chymosin / Q000737:chemistry / Q000302:isolation & purification*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D012085:Chymosin