title: The disodium salt of EDTA inhibits the binding of vasoactive intestinal peptide to macrophage membranes: endodontic implications.
abstract: The purpose of this study was to investigate the effect of the disodium salt of ethylenediamine tetraacetate (EDTA), a calcium ion chelator used in the root canal therapy, on vasoactive intestinal peptide (VIP) binding to macrophage membranes (MM's). Binding assays were conducted at 15 degrees C in 0.5 ml of 50 mM Tris-HCl buffer (pH 7.5) containing 1.6% (w/v) bovine serum albumin, 1.2 mg/ml of bacitracin, and different EDTA concentrations, using 45 pM of [125I]VIP as tracer. Results showed that EDTA inhibits VIP binding to MM's in a dose-dependent manner, with an IC50 value of 5.4 mM (p < 0.01). EDTA concentrations equal or higher than 100 mM of abolished VIP-MM interaction. Taking into account that the macrophage plays an essential role in inflammatory reactions and the immune response, we conclude that the apical extrusion of EDTA during root canal therapy could modify VIP-macrophage interaction modulating the inflammatory mechanisms involved in periapical lesions.
authors.affiliation: Department of Medical Biochemistry, University of Seville, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Segura
authors.forename: J R
authors.initials: JR
authors.lastname: Calvo
authors.forename: J M
authors.initials: JM
authors.lastname: Guerrero
authors.forename: C
authors.initials: C
authors.lastname: Sampedro
authors.forename: A
authors.initials: A
authors.lastname: Jimenez
authors.forename: R
authors.initials: R
authors.lastname: Llamas
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D015213:Neuroimmunomodulation / Q000187:drug effects*; D010485:Periapical Periodontitis / Q000276:immunology*; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D014660:Vasoactive Intestinal Peptide; D004533:Egtazic Acid; D004492:Edetic Acid