Outer membrane protein of Neisseria meningitidis as a mucosal adjuvant for lipopolysaccharide of Brucella melitensis in mouse and guinea pig intranasal immunization models.
A mucosal vaccine against brucellosis consisting of the lipopolysaccharide (LPS) of Brucella melitensis complexed with the outer membrane protein (GBOMP) of group B Neisseria meningitidis was tested in small-animal models of intranasal immunization. Mice given two doses of the vaccine developed high levels of immunoglobulin G (IgG) and IgA antibodies specific for B. melitensis LPS in lung lavages and specific IgG and IgA antibody-secreting cells in the lungs and spleen. Similarly, in guinea pigs immunized twice intranasally, IgG and IgA LPS-specific antibodies were detected in lung lavages, and specific antibody-secreting cells were isolated from the spleen and cervical nodes. In mice immunized with LPS only, pulmonary responses consisted mostly of IgM antibodies, while guinea pigs given LPS alone developed local antibody of all three isotypes, but at lower levels compared to animals given the complex vaccine. Both mice and guinea pigs also developed high levels of serum IgG and moderate levels of IgA as a result of intranasal immunization with the complex vaccine. The serum antibodies in both cases were found to cross-react with the LPS of B. abortus, which shares an immunogenic epitope with B. melitensis LPS. In mice given the complex vaccine, there was a prominent serum IgG1 response that was absent in the mice given LPS alone. In conclusion, the N. meningitidis GBOMP was an effective mucosal adjuvant for secretory IgA and IgG responses in the lungs of both mice and guinea pigs. The IgG1 subclass response in mice suggests that GBOMP may have favored a Th2 type of response to the LPS. A vaccine capable of stimulating high levels of antibody at local sites has the potential to protect against brucellae, since these pathogens gain entry to the host via mucosal routes.
Van De Verg
L L
LL
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20307, USA.
Hartman
A B
AB
Bhattacharjee
A K
AK
Tall
B D
BD
Yuan
L
L
Sasala
K
K
Hadfield
T L
TL
Zollinger
W D
WD
Hoover
D L
DL
Warren
R L
RL
D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides