title: Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes.
abstract: The preferred positions for meiotic double-strand breakage were mapped on Saccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulsed-field gel electrophoresis per 25 kb of DNA, but each chromosome had a unique distribution of DSB sites. There were no preferred meiotic DSB sites near the telomeres. DSB-prone regions were associated with all of the known "hot spots" for meiotic recombination on chromosomes I, III and VI.
authors.affiliation: Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Klein
authors.forename: D
authors.initials: D
authors.lastname: Zenvirth
authors.forename: V
authors.initials: V
authors.lastname: Dror
authors.forename: A B
authors.initials: AB
authors.lastname: Barton
authors.forename: D B
authors.initials: DB
authors.lastname: Kaback
authors.forename: G
authors.initials: G
authors.lastname: Simchen
mesh_terms: D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004274:DNA, Recombinant; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere
chemical_list: D004274:DNA, Recombinant