Effect of Helicobacter pylori lipopolysaccharide (LPS) and LPS derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells.
Different Helicobacter pylori lipopolysaccharides (LPSs) and LPS-derivatives were studied for their ability to induce the production of procoagulant activity (PCA) and plasminogen activator inhibitor type 2 (PAI-2) by human blood mononuclear leukocytes. Smooth (S)- and rough (R)-form LPSs caused a similar increase in cell-associated PCA (tissue factor) and PAI-2 antigen release. Both effects were potentiated by fetal bovine serum via a CD14-mediated mechanism. The potency of H. pylori LPSs was approximately 1000-fold lower than that of Salmonella typhimurium LPSs. When H. pylori LPS derivatives (dephosphorylated R-LPS, S-lipid A, and R-lipid A) were used, PCA and PAI-2 production were markedly reduced. R-lipid A was approximately 4-fold less efficient than S-lipid A. These findings suggest that the induction of monocyte tissue factor and PAI-2 by H. pylori LPS is influenced by the lipid A structure and modulated by the core oligosaccharide and that phosphate groups present in both regions may play an important role.
Semeraro
N
N
Department of Biomedical Sciences and Human Oncology, Section of General Pathology and Institute of Medical Microbiology, University of Bari, Italy.
Montemurro
P
P
Piccoli
C
C
Muolo
V
V
Colucci
M
M
Giuliani
G
G
Fumarola
D
D
Pece
S
S
Moran
A P
AP
D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000737:chemistry* / Q000472:pathogenicity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000502:physiology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017396:Plasminogen Activator Inhibitor 2 / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013925:Thromboplastin / Q000096:biosynthesis*
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D017396:Plasminogen Activator Inhibitor 2; D012710:Serum Albumin, Bovine; D013925:Thromboplastin