Minimally modified oligonucleotides - combination of end-capping and pyrimidine-protection.
The nuclease resistance of an oligonucleotide sequence that was phosphorothioate (PS)-modified in various positions and patterns was examined. We present a new ¿minimal' protection strategy for antisense oligonucleotides which is a combination of the end-capping technique and the protection of internal pyrimidine residues which are the major sites of endonuclease degradation. This strategy reduces the number of modifications needed to make a nuclease resistant oligonucleotide and therefore should minimize the non-sequence-specific effects that are frequently observed with uniformly modified oligonucleotides.
Peyman
A
A
Hoechst AG, Pharma Research, Frankfurt, Germany.
Uhlmann
E
E
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
D016376:Oligonucleotides, Antisense; D011743:Pyrimidines