title: Infection of T84 cells with enteropathogenic Escherichia coli alters barrier and transport functions.
abstract: The effect of enteropathogenic Escherichia coli (EPEC) infection on electrophysiology of T84 cell monolayers was examined. After 18 h of infection with EPEC (E2348), transepithelial electrical resistance was decreased (30 +/- 5% of uninfected values) compared with monolayers infected with a nonpathogenic E. coli strain (104 +/- 13%). Resistance of monolayers infected with EPEC mutant strain CVD206, deficient in attaching and effacing lesion formation, was partially reduced (66 +/- 10%). In addition, permeability of EPEC-infected T84 monolayers increased compared with uninfected cells. Associated with these changes was an altered distribution of the tight junction protein, ZO-1. Taken together, these findings suggest that the barrier defect induced by EPEC was at the level of the tight junction. Adenosine 3'5'-cyclic monophosphate-stimulated chloride secretion was also diminished in EPEC-infected cells, whereas Ca2+ -dependent chloride secretion was not different from uninfected cells. These findings indicate that EPEC infection alters intestinal epithelial barrier and transport functions. Furthermore, these results provide a possible mechanism for EPEC-induced diarrheal disease.
authors.affiliation: Division of Gastroenterology, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: D J
authors.initials: DJ
authors.lastname: Philpott
authors.forename: D M
authors.initials: DM
authors.lastname: McKay
authors.forename: P M
authors.initials: PM
authors.lastname: Sherman
authors.forename: M H
authors.initials: MH
authors.lastname: Perdue
mesh_terms: D000199:Actins / Q000378:metabolism; D001422:Bacterial Adhesion; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D002712:Chlorides / Q000378:metabolism; D004594:Electrophysiology; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007410:Intestinal Diseases / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007422:Intestines / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010539:Permeability; D010750:Phosphoproteins / Q000378:metabolism; D062826:Zonula Occludens-1 Protein
chemical_list: D000199:Actins; D002712:Chlorides; D008565:Membrane Proteins; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D002118:Calcium