Identification of an amphotericin B resistant strain of Candida albicans using a rapid 3H-glucose incorporation microassay.
Using a 3H-glucose incorporation assay, antifungal sensitivity testing undertaken on an isolate of Candida albicans cultured from the blood of a bone marrow transplant patient documented resistance to amphotericin B but sensitivity to fluconazole and itraconazole. Information obtained from in vitro antifungal sensitivity testing can be used to direct in vivo antifungal therapy. Widespread application of standardized in vitro antifungal sensitivity testing is needed.
Sweeney
J F
JF
Department of Surgery, University of South Florida, College of Medicine, Tampa, USA.
Greene
J N
JN
Hiemenz
J W
JW
Wei
S
S
Rosemurgy
A S
AS
Djeu
J Y
JY
D000328:Adult; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D016867:Immunocompromised Host; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests
D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole