A new method for permeabilization of cultured cells without cell damage.
Transient permeabilization was readily achieved by vortex-stirring cultured mammalian cells for a few seconds in the presence of 1 to 10 micrograms/ml of high molecular weight polyacrylic acid (PAA). This was demonstrated by an appreciable uptake by murine leukemia L1210 cells of Lucifer Yellow (LY), which is known to be a non-permeant except under conditions in which micropores are induced in the plasma membrane. In addition, the cytotoxicity of bleomycin (BLM), a very poor permeant, was markedly enhanced by vortex-stirring with PAA. Scope and limitation of this permeabilization procedure is preliminarily described.
Shimizu
N
N
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Kawazoe
Y
Y
D000180:Acrylic Resins / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D000970:Antineoplastic Agents / Q000494:pharmacology; D001761:Bleomycin / Q000633:toxicity; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007546:Isoquinolines; D007939:Leukemia L1210 / Q000473:pathology; D051379:Mice
D000180:Acrylic Resins; D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D007546:Isoquinolines; D001761:Bleomycin; C006903:carbopol 940; C017475:lucifer yellow