Cloning and sequence analysis of laccase-encoding cDNA clones from tobacco.
Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA library. One of them contains a full length sequence coding for a cationic laccase. The predicted polypeptide sequence shows 48% identity with sycamore laccase. Amino acid comparisons with other laccases and ascorbate oxidases have shown that this new plant laccase sequence also contains four potential copper binding regions which are highly conserved among the blue copper oxidases.
Kiefer-Meyer
M C
MC
Laboratoire des Transports Intracellulaires, CNRS URA 203, Université de Rouen, Mont-Saint-Aignan, France.
Gomord
V
V
O'Connell
A
A
Halpin
C
C
Faye
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant; D042845:Laccase; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D018744:DNA, Plant; D010088:Oxidoreductases; D042845:Laccase