Molecular cloning and characterization of EPI-1, the major protein in chimpanzee (Pan troglodytes) cauda epididymal fluid.
A 27-kDa glycoprotein comprises approximately 20% of the total protein in chimpanzee (Pan troglodytes) cauda epididymal fluid. Polyclonal antibodies generated against this glycoprotein react with 27- and 25-kDa components in chimpanzee cauda epididymal fluid and in human, gorilla, chimpanzee, and monkey seminal fluid. According to microsequencing, the 27- and 25-kDa components (chimpanzee EPI-1) are identical to the cloned putative human epididymal protein HE1. Screening of a chimpanzee epididymal cDNA library enabled isolation of a cDNA clone of chimpanzee EPI-1. On the cDNA level, chimpanzee EPI-1 and human HE1 are 99% identical. Northern analysis localized chimpanzee EPI-1 mRNA to the distal caput epididymidis. With EPI-1 primers, polymerase chain reaction of reverse-transcribed rhesus monkey (Macaca mulatta) epididymal RNA enabled isolation of a rhesus monkey EPI-1 cDNA clone. The derived amino acid sequence of rhesus monkey EPI-1 is identical to chimpanzee EPI-1 and to human HE1. Northern analysis localized rhesus monkey EPI-1 mRNA to the distal caput and the proximal corpus epididymidis. Northern analysis also showed that chimpanzee EPI-1 and rhesus monkey EPI-1 gene products are expressed specifically in the epididymis and not in any other tissue examined.
Fröhlich
O
O
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Young
L G
LG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001826:Body Fluids / Q000378:metabolism; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D006023:Glycoproteins; C104585:NPC2 protein, human; D033921:Vesicular Transport Proteins