The DNA polymerase-encoding gene from a thermoacidophilic archaeon Sulfolobus acidocaldarius.
We have cloned, sequenced and characterized the gene encoding a DNA polymerase from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sac). The putative transcription promoter and terminator elements, as well as a potential ribosome-binding site (rbs), have been identified in the flanking regions. One large open reading frame (ORF) found in the sequenced portion of the Sac genome encodes a protein of 875 amino acids (aa). All conserved motifs characteristic of family B of DNA polymerases have been found in the deduced primary structure of this enzyme. The Sac DNA polymerase also contains sequence motifs that form a proofreading exonuclease domain.
Datukishvili
N
N
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Pokholok
D
D
Lottspeich
F
F
Prangishvili
D
D
Rechinsky
V
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D008969:Molecular Sequence Data; D016945:Sulfolobus acidocaldarius / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase