Gelation kinetics of dilute hemoglobin from sickle cell anemia patients.
Non-ideal behavior of sickle cell hemoglobin (Hb S) and varying experimental conditions have made gelling kinetic studies difficult. A new gelation kinetic procedure is developed in this study. This technique uses low Hb S concentrations (20-fold less than in conventional methods) without the need for a temperature jump. Gelation progress, monitored with a newly developed turbidimetric procedure at 815 nm, was fitted to a mono-exponential and a sigmoid-Emax models. These models allowed precise definitions of gelation delay periods, rate of rapid Hb S gelation and a parameter, T 1/2 which combines information from the delay and rapid gelation stages. All these parameters vary linearly with Hb S concentration. The merits of using T 1/2 are discussed.
Fasanmade
A A
AA
Department of Medicine, University of Chicago, IL 60637, USA.
D000755:Anemia, Sickle Cell / Q000097:blood*; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D009391:Nephelometry and Turbidimetry
D006451:Hemoglobin, Sickle