title: What we have learned about bumetanide and the concept of multispecific bile acid/drug transporters from the liver.
abstract: Bumetanide is a weak organic acid which is transported into hepatocytes by a transport system that is related neither to the cloned sodium-dependent taurocholate cotransporting polypeptide Ntcp nor to the cloned organic anion transporting polypeptide oatp. Bumetanide is known to be transported in the kidney by a multispecific organic anion transporter which is the pAH-transporter from the proximal tubule cell. In the liver, bumetanide uptake competes with bile acid uptake, indicating a functionally related multispecific transporter for bile acids and drugs in hepatocytes. This multispecific bile acid transporter MBAT has not been cloned yet. When basolateral membranes were photoaffinity labeled with [3H]bumetanide, several bumetanide binding proteins were separated and identified after protein sequencing from two-dimensional electrophoresis gels.
authors.affiliation: Institute of Pharmacology and Toxicology, Justus Liebig University Giessen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Petzinger
authors.forename: M
authors.initials: M
authors.lastname: Blumrich
authors.forename: B
authors.initials: B
authors.lastname: Brühl
authors.forename: U
authors.initials: U
authors.lastname: Eckhardt
authors.forename: W
authors.initials: W
authors.lastname: Föllmann
authors.forename: W
authors.initials: W
authors.lastname: Honscha
authors.forename: J A
authors.initials: JA
authors.lastname: Horz
authors.forename: N
authors.initials: N
authors.lastname: Müller
authors.forename: L
authors.initials: L
authors.lastname: Nickau
authors.forename: M
authors.initials: M
authors.lastname: Ottallah-Kolac
authors.forename: H D
authors.initials: HD
authors.lastname: Platte
authors.forename: A
authors.initials: A
authors.lastname: Schenk
authors.forename: K
authors.initials: K
authors.lastname: Schuh
authors.forename: K
authors.initials: K
authors.lastname: Schulz
authors.forename: S
authors.initials: S
authors.lastname: Schulz
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D002034:Bumetanide / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D028021:Sodium-Potassium-Chloride Symporters
chemical_list: D001647:Bile Acids and Salts; D002352:Carrier Proteins; D004337:Drug Carriers; D028021:Sodium-Potassium-Chloride Symporters; D002034:Bumetanide; C017199:2',3'-dialdehyde ATP; D000255:Adenosine Triphosphate