Magnetizable solid-phase supports for purification of nucleic acids.
A simple, small scale non-hazardous procedure for the production of magnetizable solid-phase support (MSPS) beads has been developed based on the extrusion of agarose/iron oxide mixtures. The MSPS beads were derivatized using various amine ligands. Derivatized MSPS beads were used to adsorb nucleic acids from aqueous solution and to separate RNA/DNA mixtures.
Bruce
I J
IJ
School of Biological and Chemical Sciences, University of Greenwich, London, UK.
Davies
M J
MJ
Howard
K
K
Smethurst
D E
DE
Todd
M
M
D002847:Chromatography, Agarose / Q000379:methods*; D008280:Magnetics; D009696:Nucleic Acids / Q000302:isolation & purification*; D012685:Sepharose / Q000648:ultrastructure
D009696:Nucleic Acids; D012685:Sepharose