Peptide display on functional tailspike protein of bacteriophage P22.
The tailspike protein (TSP) of Salmonella typhimurium P22 bacteriophage is a multifunctional homotrimer, 6 copies of which are non-covalently attached to the capsid to form the virion tail in the last reaction of phage assembly. An antigenic peptide of foot-and-mouth disease virus (FMDV), aa 134-156 of protein VP1, has been joined to the carboxy terminus of TSP, and produced as a fusion protein in Escherichia coli directed by the trp promoter. The resulting fusion protein is soluble, stable, non-toxic, and can be easily purified by standard procedures. Moreover, both the endorhamnosidase and capsid assembly activities of the TSP are conserved, permitting the fusion protein to reconstitute infectious viruses by in vitro association with tailless particles. In both free TSP and P22 chimeric virions, the foreign peptide is solvent-exposed and highly antigenic, indicating that P22 TSP could be an appropriate carrier protein for multimeric peptide display.
Carbonell
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Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Villaverde
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D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D017100:Bacteriophage P22 / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D015870:Gene Expression; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012486:Salmonella typhimurium / Q000821:virology; D017376:Viral Tail Proteins / Q000235:genetics*; D014771:Virion
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D010455:Peptides; D011993:Recombinant Fusion Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D017376:Viral Tail Proteins; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage