title: The SpoIIAA protein of Bacillus subtilis has GTP-binding properties.
abstract: SpoIIAA is the first protein of the spoIIA operon. Here we show that SpoIIAA can bind and hydrolyze GTP. The protein also accepts ATP, but with lower affinity. GDP competes poorly for binding of GTP. The GTPase activity of SpoIIAA is within the range found for other GTP-binding proteins.
authors.affiliation: Department of Biochemistry, University of Oxford, United Kingdom.
authors.forename: S M
authors.initials: SM
authors.lastname: Najafi
authors.forename: D A
authors.initials: DA
authors.lastname: Harris
authors.forename: M D
authors.initials: MD
authors.lastname: Yudkin
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000145:classification / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D020558:GTP Phosphohydrolases / Q000145:classification / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D019204:GTP-Binding Proteins / Q000145:classification / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism* / Q000528:radiation effects; D008274:Magnesium / Q000494:pharmacology; D012808:Sigma Factor; D014157:Transcription Factors; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D008274:Magnesium