title: Construction of a dimeric DNA-binding peptide model by peptide-anthraquinone conjugation.
abstract: A peptide-anthraquinone conjugate was designed and synthesized containing linked peptide chains composed of Asp-Pro-Ala-Ala-Leu-Lys-Arg-Ala-Arg-Asn-Thr-Glu-Ala- Ala-Arg-Arg-Ser-Arg-Ala-Arg-Lys-Leu-Gln-Arg-Met, representing the basic region of GCN4. The two peptides were joined with anthraquinone at its 1- and 8-positions in a two-fold symmetric fashion, mimicking dimeric DNA-binding proteins. Experimental data indicated both an interaction of the anthraquinone moiety with the DNA double strand and an increase in the alpha-helicity of the peptide moieties of the ligand when it binds to DNA.
authors.affiliation: Department of Chemical Science and Technology, Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takenaka
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Itakura
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry*; D008024:Ligands; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011494:Protein Kinases / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000880:Anthraquinones; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D008024:Ligands; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D011494:Protein Kinases