title: Isolation and characterization of a functional promoter from Nitrosomonas europaea.
abstract: A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at -35 and -10 have low similarity to the -10/-35 consensus sequence of known prokaryotic promoters.
authors.affiliation: Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Nejidat
authors.forename: O
authors.initials: O
authors.lastname: Spector
authors.forename: A
authors.initials: A
authors.lastname: Abeliovich
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009606:Nitrosomonas / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D000641:Ammonia