Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing.
Migration of hematopoietic precursor cells to the thymus is shown to depend upon a novel molecule called Vanin-1 expressed by perivascular thymic stromal cells. An anti-Vanin-1 antibody blocks the binding of pro-T cells to thymic sections in vitro, the in vivo accumulation of bone marrow cells around cortical thymic vessels, and long-term thymic regeneration. Thus, it interferes with the entry, and not the differentiation, of hematopoietic precursor cells. The Vanin-1 gene codes for a GPI-anchored 70 kDa protein that shows homology only with human biotinidase. Transfection of thymic stromal cells with the Vanin-1 cDNA enhances thymocyte adhesion in vitro. These data suggest that Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions.
Aurrand-Lions
M
M
Centre d'Immunologie, INSERM-Centre National de la Recherche Scientifique, Marseille, France.
Galland
F
F
Bazin
H
H
Zakharyev
V M
VM
Imhof
B A
BA
Naquet
P
P
D000581:Amidohydrolases; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology*; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008833:Microcirculation / Q000276:immunology; D008969:Molecular Sequence Data; D011828:Radiation Chimera / Q000276:immunology; D012038:Regeneration / Q000276:immunology; D013194:Staining and Labeling; D017154:Stromal Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000098:blood supply / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D007166:Immunosuppressive Agents; D000581:Amidohydrolases; C022314:pantetheinase