Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat racial motoneurons after neonatal axotomy and CNTF treatment.
Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.
Duberley
R M
RM
Department of Anatomy and Development Biology, Royal Free Hospital, School of Medicine, London, UK.
Johnson
I P
IP
D000818:Animals; D001369:Axons / Q000502:physiology*; D020934:Ciliary Neurotrophic Factor; D003714:Denervation; D005154:Facial Nerve / Q000166:cytology*; D019004:Galanin / Q000235:genetics; D017403:In Situ Hybridization; D009046:Motor Neurons / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D020801:Receptor, Ciliary Neurotrophic Factor; D020812:Receptor, trkC; D017475:Receptors, Nerve Growth Factor / Q000737:chemistry / Q000235:genetics*
D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D019004:Galanin; D020794:Receptor Protein-Tyrosine Kinases; D020812:Receptor, trkC