Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway.
Cell cycle regulation is critical for maintenance of genome integrity. A prominent factor that guarantees genomic stability of cells is p53 (ref. 1). The P53 gene encodes a transcription factor that has a role as a tumour suppressor. Identification of p53-target genes should provide greater insight into the molecular mechanisms that mediate the tumour suppressor activities of p53. The rodent Pc3/Tis21 gene was initially described as an immediate early gene induced by tumour promoters and growth factors in PC12 and Swiss 3T3 cells. It is expressed in a variety of cell and tissue types and encodes a remarkably labile protein. Pc3/Tis21 has a strong sequence similarity to the human antiproliferative BTG1 gene cloned from a chromosomal translocation of a B-cell chronic lymphocytic leukaemia. This similarity led us to speculate that BTG1 and the putative human homologue of Pc3/Tis21 (named BTG2) were members of a new family of genes involved in growth control and/or differentiation. This hypothesis was recently strengthened by the identification of a new antiproliferative protein, named TOB, which shares sequence similarity with BTG1 and PC3/TIS21 (ref. 7). Here, we cloned and localized the human BTG2 gene. We show that BTG2 expression is induced through a p53-dependent mechanism and that BTG2 function may be relevant to cell cycle control and cellular response to DNA damage.
Rouault
J P
JP
Unité INSERM U453, Affiliée au CNRS, Centre Léon Bérard, Lyon, France.
Falette
N
N
Guéhenneux
F
F
Guillot
C
C
Rimokh
R
R
Wang
Q
Q
Berthet
C
C
Moyret-Lalle
C
C
Savatier
P
P
Pain
B
B
Shaw
P
P
Berger
R
R
Samarut
J
J
Magaud
J P
JP
Ozturk
M
M
Samarut
C
C
Puisieux
A
A
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004249:DNA Damage; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D017874:Immediate-Early Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D025521:Tumor Suppressor Proteins
C489552:Btg1 protein, mouse; C489435:Btg2 protein, mouse; D017874:Immediate-Early Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C070114:BTG2 protein, human; C074086:BTG1 protein, human