title: Borrelia burgdorferi induces secretion of pro-urokinase-type plasminogen activator by human monocytes.
abstract: Borrelia burgdorferi is transmitted by infected ticks and causes Lyme disease. To infect distant organ sites, B. burgdorferi spirochetes must disseminate from the site of the tick bite. During dissemination from the dermal tissue, they breach tissue barriers, probably by proteolysis. The previous findings that spirochetes bind serum-derived plasminogen and that plasmin favors spirochetal invasiveness and infectivity suggested a role for plasmin in the pathogenicity of B. burgdorferi. Binding of plasminogen to spirochetes and activation into plasmin is favored in a microenvironment that is rich in plasminogen and plasminogen activators. Plasminogen is abundant in plasma and interstitial fluids, and it is increased in inflammatory exudates. Since B. burgdorferi does not express endogenous plasminogen activators, the conversion of spirochete-bound plasminogen depends on host-derived plasminogen activators. In this report, we show that both intact B. burgdorferi organisms and its recombinant outer surface lipoprotein A induce human monocytes to express and secrete urokinase-type plasminogen activator in its zymogen form (pro-uPA). Moreover, we demonstrate that the presence of B. burgdorferi accelerates the interaction between (pro-)uPA and plasmin(ogen), leading to spirochete-bound plasmin. In a pro-uPA-serum mixture, spirochete-bound plasmin activity is generated. Taken together, the data suggest that B. burgdorferi may induce pro-uPA in a monocyte-containing inflammatory site and that the spirochetal surface provides an appropriate milieu for subsequent interactions between (pro-)uPA and plasmin(ogen), which result in spirochete-bound plasmin even in the presence of inhibitors for plasminogen activators and plasmin.
authors.affiliation: Institute for Immunology, Laboratory for Immunopathology, University of Heidelberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Fuchs
authors.forename: M M
authors.initials: MM
authors.lastname: Simon
authors.forename: R
authors.initials: R
authors.lastname: Wallich
authors.forename: M
authors.initials: M
authors.lastname: Bechtel
authors.forename: M D
authors.initials: MD
authors.lastname: Kramer
mesh_terms: D015748:Borrelia burgdorferi Group / Q000502:physiology*; D002460:Cell Line; D004792:Enzyme Precursors / Q000378:metabolism*; D005341:Fibrinolysin / Q000096:biosynthesis; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D010958:Plasminogen / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D010958:Plasminogen; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator; C067551:saruplase