Synthesis and biochemical activity of novel amidine derivatives as m1 muscarinic receptor agonists.
As part of a continuing effort aimed at the development of selective, efficacious, and centrally active m1 muscarinic agonists for the treatment of Alzheimer's disease, a series of amide and hydrazide amidine derivatives (2a-e and 3b-d) was synthesized and examined for muscarinic agonist activity. Preliminary biochemical studies indicated that 2b, 2d, and 3d bound to muscarinic receptors in rat brain and stimulated phosphoinositide (PI) metabolism in rat cerebral cortex. Compounds 2b and 2d were also highly efficacious at m1 muscarinic receptors expressed in cultured A9 L cells. Molecular modeling studies suggest slightly different modes of interaction with m1 receptors for the ester and amide derivatives. Also, hydrogen-bond formation with a Thr residue may be important for m1 muscarinic agonist potency. The data suggest that the amide moiety can replace the ester group found in muscarinic agonists and provide further support for the utility of amidine derivatives in the development of efficacious m1 agonists.
Ojo
B
B
Department of Medicinal and Biological Chemistry, College of Pharmacy, University of Toledo, OH 43606, USA.
Dunbar
P G
PG
Durant
G J
GJ
Nagy
P I
PI
Huzl
J J
JJ
Periyasamy
S
S
Ngur
D O
DO
el-Assadi
A A
AA
Hoss
W P
WP
Messer
W S
WS
D000578:Amidines / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D007700:Kinetics; D008958:Models, Molecular; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic / Q000378:metabolism*
D000578:Amidines; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate