The AMOG/beta 2 subunit of Na,K-ATPase is not necessary for long-term survival of telencephalic grafts.
Adhesion molecule on glia (AMOG) represents the beta 2-subunit of murine Na,K-ATPase. Mice carrying a targeted deletion of the AMOG/beta 2 gene exhibit tremor and limb paralysis at postnatal day (P) 15 and die 2 days after the onset of symptoms. The brains of these mice show edema and swelling of astrocytic end feet. However, the cause of death has remained unclear. To identify long-term consequences of AMOG/beta 2 deficiency, we have grafted parts of the embryonic telencephalic anlage of AMOG/beta 2-deficient mice into the caudoputamen of wild-type mice and analyzed the grafts up to 500 days after transplantation. Histological, immunocytochemical, and in situ hybridization techniques were applied to examine histoarchitecture, proliferation, differentiation, and long-term survival of grafts. AMOG/beta 2-deficient telencephalic grafts develop normally and form solid neural tissue that cannot be distinguished from control grafts by morphological features or with immunocytochemical stains for neuronal and glial markers. No signs of degeneration can be found. Expression analysis, however, revealed that no AMOG/beta 2 protein of possible host origin can be detected in AMOG/beta 2-deficient grafts. Graft-borne astrocytes express neither the AMOG/beta 1 nor the AMOG/beta 2 subunit of Na,K-ATPase as examined with immunocytochemistry and in situ hybridization. These findings indicate that AMOG/beta 2 is not necessary for long-term survival of telencephalic graft tissue.
Isenmann
S
S
Institute of Neuropathology, University Hospital, Zürich, Switzerland.
Molthagen
M
M
Brandner
S
S
Bartsch
U
U
Kühne
G
G
Magyar
J P
JP
Sure
U
U
Schachner
M
M
Aguzzi
A
A
D000251:Adenosine Triphosphatases; D000818:Animals; D016380:Brain Tissue Transplantation / Q000473:pathology; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D006085:Graft Survival / Q000187:drug effects*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000502:physiology*; D013687:Telencephalon / Q000201:enzymology* / Q000473:pathology / Q000637:transplantation*
C504191:Atp1b2 protein, mouse; C504192:Atp1b2 protein, rat; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase