African swine fever virus gene A179L, a viral homologue of bcl-2, protects cells from programmed cell death.
The African swine fever virus (ASFV) open reading frame A179L, which is similar to the human proto-oncogene bcl-2, has been cloned and expressed in vaccinia virus under control of the pEIL synthetic early/late promoter. The A179L gene product prevented cell death in HeLa and BSC-40 cells doubly infected with another recombinant vaccinia virus expressing the interferon-induced double-stranded RNA-activated protein kinase (p68 kinase), which activates a rapid cell death characteristic of apoptosis. This finding suggests that the A179L gene has a function similar to that of bcl-2 in preventing apoptosis and may play an important role during productive ASFV infection.
Brun
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Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain.
Rivas
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Esteban
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Escribano
J M
JM
Alonso
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D000358:African Swine Fever Virus / Q000235:genetics*; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D053938:DNA Fragmentation; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics / Q000502:physiology*; D019254:Genes, bcl-2 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology; D000090063:Proto-Oncogene Mas; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014616:Vaccinia virus / Q000235:genetics; D019892:eIF-2 Kinase
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase