The turkey c-rap1A proto-oncogene is expressed via two distinct promoters.
The turkey c-K-ras(B) transcript and two species of the turkey rap1A transcripts transcribed from two distinct promoters were isolated from a turkey spleen cDNA library. Turkey K-Ras and Rap1A proteins shared extensive amino acid (aa) sequence relatedness throughout their major functional domains: the four GTP-binding domains, the effector region and the C-terminal CAAX box. However, they diverged significantly in their intervening regions. In contrast, almost complete identity in the aa composition was exhibited between turkey K-Ras and Rap1A and their human homologues. The complete conservation that exists between turkey and human Rap1A, also along the polybasic C-terminal domain as opposed to Rap1B, suggests the functional relevance of these divergent residues in specifying the distinct biological functions of these two closely related proteins.
Chajut
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Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Gazit
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Yaniv
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics*; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014422:Turkeys; D020668:rap GTP-Binding Proteins
D018076:DNA, Complementary; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins