Interaction of Rabphilin3 with synaptic vesicles through multiple regions.
Rabphilin3 is a downstream target protein of Rab3A small G protein, implicated in neurotransmitter release. We have previously reported that about 4 molecules of Rabphilin3 are associated with one synaptic vesicle through a vesicle anchoring protein(s). We have shown here that Rabphilin3 interacts with rat synaptic vesicles through at least three regions: the N-terminal region (1-280 amino acids (aa)), the C-terminal region containing the two C2 domains (440-704 aa), and an unidentified region. A model for the interaction of Rabphilin3 with the vesicles is discussed.
Senbonmatsu
T
T
Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Shirataki
H
H
Jin-no
Y
Y
Yamamoto
T
T
Takai
Y
Y
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016324:Sequence Tagged Sites; D018411:Spodoptera; D013572:Synaptic Vesicles / Q000378:metabolism*; D014162:Transfection; D014357:Trypsin; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A
D048868:Adaptor Proteins, Signal Transducing; D019204:GTP-Binding Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014357:Trypsin; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A