Stem cell factor-induced expression of p27kip-1 during hematopoietic differentiation.
The canine marrow stroma-derived CD34- DR- cell line D064 is able to differentiate into cells with characteristics of hematopoietic progenitors. While differentiating, these cells start to express CD34 and DR. Differentiation is induced by stem cell factor (SCF), while interleukin-6 (IL-6) inhibits differentiation, but promotes proliferation. The influence of SCF and IL-6 on differentiation and proliferation is reflected in the expression of p27kip-1. SCF induces a transient expression of p27kip-1, while no p27kip-1 is detectable in the presence of IL-6 or an anti-SCF monoclonal antibody. The accumulation of intracellular p27kip-1 precedes the differentiation of D064 cells. As these cells start to proliferate and more committed progenitors emerge, p27kip-1 is no longer expressed.
Huss
R
R
Institute of Pathology, University of Munich, Germany.
Firpo
E
E
Deeg
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D002460:Cell Line; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004285:Dogs; D004791:Enzyme Inhibitors / Q000378:metabolism*; D006410:Hematopoiesis / Q000502:physiology*; D015151:Immunoblotting / Q000379:methods; D015850:Interleukin-6 / Q000008:administration & dosage; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D019089:Stem Cell Factor / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology; D025521:Tumor Suppressor Proteins
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D015850:Interleukin-6; D008869:Microtubule-Associated Proteins; D019089:Stem Cell Factor; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27