title: Immunoaffinity column cleanup and liquid chromatographic method for determining ivermectin in sheep serum.
abstract: A simple and sensitive method was developed for determining ivermectin in sheep serum. The sample is cleaned up on an immunoaffinity column and determined by liquid chromatography with detection at 245 nm. Recoveries ranged from 90.3 to 98.8% (93.9 +/- 4.3%) for samples fortified with 2.0-50 ng ivermectin/mL. Coefficients of variation were 3.2-11.0%. The limit of detection was 1 ng/mL in 5.0 mL serum.
authors.affiliation: Beijing Agricultural University, College of Veterinary Medicine, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000097:blood*; D000918:Antibody Specificity; D002138:Calibration; D002853:Chromatography, Liquid; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification; D007164:Immunosorbents / Q000737:chemistry; D007559:Ivermectin / Q000097:blood*; D012015:Reference Standards; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000871:Anthelmintics; D007074:Immunoglobulin G; D007164:Immunosorbents; D007559:Ivermectin