IL-1 receptor antagonist in saliva; characterization in normal saliva and reduced concentration in Sjögren's syndrome (SS).
The characterization of a salivary factor cross-reacting with IL-1 receptor antagonist (IL-1Ra) is described. The apparent molecular weights of two species were 23 kD, consistent with the secreted peptide (sIL-1Ra), and 20 kD, consistent with the intracellular peptide (icIL-1Ra). It had an inhibitory activity on IL-1-stimulated fibroblasts, which is characteristic of IL-1Ra. Its source was the oral mucosa and not the salivary glands. Saliva from patients with SS contained significantly less IL-1Ra than saliva from controls. The decrease was marked in patients with early dental loss but whose xerostomia was still partial. In SS, the salivary IL-1/IL-1Ra imbalance may promote inflammatory lesions in the mouth and impede mucosal cell differentiation.
Dubost
J J
JJ
Department of Rheumatology, Hôpital Gabriel Montpied, Clermont-Ferrand, France.
Perrier
S
S
Afane
M
M
Viallard
J L
JL
Roux-Lombard
P
P
Baudet-Pommel
M
M
Begue
C
C
Kemeny
J L
JL
Sauvezie
B
B
D015153:Blotting, Western; D002850:Chromatography, Gel; D015232:Dinoprostone / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D008970:Molecular Weight; D009061:Mouth Mucosa / Q000737:chemistry; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012859:Sjogren's Syndrome / Q000378:metabolism*
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D015232:Dinoprostone