title: Separation of major polar lipids in Pecten maximus by high-performance liquid chromatography and subsequent determination of their fatty acids using gas chromatography.
abstract: An easy method for the separation of major polar lipid classes by HPLC is described. Maximum resolution was achieved by an automated combination of a silica gel column and a diol column. Polar lipid analysis of the larvae and gonads of Pecten maximus showed the presence of a particular glycolipid especially rich in 22:6(n - 3) and the predominance of 20:4(n - 6) in the phosphatidylinositol. The phosphatidylcholine and phosphatidylethanolamine (diacyl form + alkenylacyl) were the major fractions. The plasmalogen form (25% in larvae, 34% in gonads) was essentially composed of polyunsaturated fatty acids of 20 and 22 carbons in the sn-2 position.
authors.affiliation: IFREMER Centre de Brest, Plouzané, France.
authors.forename: P
authors.initials: P
authors.lastname: Soudant
authors.forename: Y
authors.initials: Y
authors.lastname: Marty
authors.forename: J
authors.initials: J
authors.lastname: Moal
authors.forename: J F
authors.initials: JF
authors.lastname: Samain
mesh_terms: D000818:Animals; D002138:Calibration; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry; D006066:Gonads / Q000737:chemistry; D007814:Larva / Q000737:chemistry; D008246:Lysophospholipids / Q000032:analysis; D008974:Mollusca / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D006017:Glycolipids; D008246:Lysophospholipids; D010743:Phospholipids