title: Confirmation of the Syva MicroTrak enzyme immunoassay for chlamydia trachomatis by Syva Direct Fluorescent Antibody Test.
abstract: OBJECTIVE
  The Syva Micro Trak enzyme immunoassay (EIA) is used widely for screening women infected with Chlamydia trachomatis. Confirmatory tests used in conjunction with EIA screening have shown that false-positive results are common.
  
  
  OBJECTIVE
  To evaluate the specificity of the Syva MicroTrak EIA by confirmation of positive specimens with the Syva Direct Fluorescent Specimen Test.
  
  
  METHODS
  Of 6,039 endocervical specimens collected from women attending Colorado family planning clinics, 328 positive EIA results (5.4%) were obtained by Syva MicroTrak EIA. A random subset of 136 positive specimens was tested by Syva Direct Specimen Test. Twenty of 136 specimens (14.7%) negative by Syva Direct Specimen testing were also tested by Syva blocking antibody tests (9 of 20 positive, 45%) and Roche Amplicor polymerase chain reaction (PCR; 6 of 20 positive, 30%). Of 20 specimens positive by Syva MicroTrak EIA and negative by Syva Direct Specimen Test, 11 (55%) were also negative by blocking antibody and PCR, including three specimens with initial EIA sample-to-cutoff ratios greater than 2.
  
  
  CONCLUSIONS
  Confirmatory testing of Syva MicroTrak EIA positive specimens with Syva Direct Specimen Test showed that 14.7% were false positive. Coupling the Syva Direct Specimen test with either blocking antibody or PCR reduces the rate of false-positive results to 8%.
authors.affiliation: Colorado Department of Public Health and Environment, University of Colorado Health Sciences Center, Denver, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Beebe
authors.forename: H
authors.initials: H
authors.lastname: Masters
authors.forename: D
authors.initials: D
authors.lastname: Jungkind
authors.forename: D M
authors.initials: DM
authors.lastname: Heltzel
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005189:False Positive Reactions; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002575:Uterine Cervicitis / Q000175:diagnosis / Q000382:microbiology*