title: cDNA cloning of a 20-kDa protein (p20) highly homologous to small heat shock proteins: developmental and physiological changes in rat hindlimb muscles.
abstract: A cDNA clone encoding p20, a novel member of the small heat-shock protein family in mammals, was isolated from a rat soleus cDNA library. The clone contained an insert of 1.3 kb with an open reading frame specifying a polypeptide of 162 amino-acid residues. Southern blot analysis suggested that the p20 gene is a single gene in rat genome. Developmental changes and a sciatic nerve denervation experiment suggested that the expression of p20 in rat hindlimb muscle is related to muscle contraction, and specifically in slow-twitch muscles.
authors.affiliation: Department of Biochemistry, Aichi Human Service Center, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inaguma
authors.forename: K
authors.initials: K
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Nishida
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003714:Denervation; D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D006614:Hindlimb; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000196:embryology / Q000294:innervation / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D009124:Muscle Proteins; C087458:Hspb6 protein, rat