title: Accelerated evolution of crotalinae snake venom gland serine proteases.
abstract: Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.
authors.affiliation: Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Deshimaru
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: I
authors.initials: I
authors.lastname: Nobuhisa
authors.forename: T
authors.initials: T
authors.lastname: Chijiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Shimohigashi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumaki
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: I
authors.initials: I
authors.lastname: Yamashina
authors.forename: S
authors.initials: S
authors.lastname: Hattori
authors.forename: M
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012103:Batroxobin / Q000737:chemistry / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000201:enzymology*; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D017841:Trimeresurus / Q000235:genetics*
chemical_list: D003435:Crotalid Venoms; D018076:DNA, Complementary; C029748:Trimeresurus venoms; D012103:Batroxobin; D012697:Serine Endopeptidases; C057255:flavoxobin