Bone mineral density and bone markers in relation to vitamin D receptor gene polymorphisms in Chinese men and women.
Whether vitamin D receptor gene (VDRG) polymorphism can be used as a predictor for bone turnover rate or bone mass remains controversial. Its role within various ethnic populations are also unsettled. We examined VDRG polymorphism using restrictive enzymes Bsm-I, Apa-I, and Taq-I in 155 men aged 22-88 and 113 premenopausal women aged 40-53. The bone mineral density (BMD) of the vertebrae (L2-4), proximal femur, and total body bone mineral content (tb-BMC) (women only), as well as urinary N-terminal crosslinked fragment of type I collagen (NTX), serum osteocalcin, bone isozyme of alkaline phosphatase, and caboxyterminal propeptide of type I procollagen levels were measured. Chinese men and women exhibited a low prevalence for B (absence of Bsm-I restriction site) phenotypes than white and Japanese. Within the tested samples there were 0.4% BB homozygotes, 6.7% Bb heterozygotes, and 93% bb homozygotes. The distributions of Apa-I polymorphism (9.0% AA, 42.5% Aa, and 48.5% aa) also differed from those reported for the white populations. Most of the Chinese men and women were TT homozygous (96.6%). A comparison of actual values and values adjusted for age and weight of tb-BMC and BMD at the lumbar spine, Trochanter, Ward's triangle, and femoral neck showed no significant difference among three subgroups in each of the three sets of polymorphism. Furthermore, the actual values and adjusted values (adjusted for age) of the four bone markers, respectively, showed no significant differences. We conclude that given the very low prevalence of the suspected high risk genotypes (B, A, and t), and the lack of difference among the polymorphic subgroups, VDRG polymorphism may not be an important determinant of the bone turnover rate and bone mass of Chinese men and women.
Tsai
K S
KS
Department of Laboratory Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.
Hsu
S H
SH
Cheng
W C
WC
Chen
C K
CK
Chieng
P U
PU
Pan
W H
WH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D015519:Bone Density / Q000235:genetics* / Q000502:physiology; D016009:Chi-Square Distribution; D002681:China; D003094:Collagen / Q000652:urine; D005260:Female; D005269:Femur / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes; D008159:Lumbar Vertebrae / Q000502:physiology; D008297:Male; D008593:Menopause; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood; D010024:Osteoporosis / Q000201:enzymology / Q000235:genetics*; D010446:Peptide Fragments / Q000378:metabolism; D011110:Polymorphism, Genetic / Q000235:genetics; D011347:Procollagen / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics*; D015183:Restriction Mapping
D007527:Isoenzymes; D010446:Peptide Fragments; D011347:Procollagen; D018167:Receptors, Calcitriol; C042813:procollagen type I carboxy terminal peptide; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase