Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus).
A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.
Blewett
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Department of Veterinary Parasitology, Microbiology and Public Health, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078-2006, USA. blewett@osuunx.ucc.okstate.edu
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Eberle
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D005944:Glucosamine / Q000378:metabolism; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; D005944:Glucosamine