title: Steroid hormones differentially induce transcription of the chicken ovalbumin gene, but stabilize the mRNA with the same half-life.
abstract: The stabilization of chicken ovalbumin (OVA) mRNA by different classes of steroid hormones (estrogen, progesterone, glucocorticoid, and androgen) was studied in the oviducts of chicks treated with combinations of four steroids. The combination of estrogen with progesterone, glucocorticoid, or androgen enhanced the induction of the OVA gene more than did estrogen alone. Run-on analysis of the isolated oviduct nuclei to measure the transcription rate of the OVA gene showed that the enhanced induction of the OVA gene by the combined hormone treatments was partly caused by an increased rate of transcription. The half-life of OVA mRNA as determined using a transcription inhibitor (actinomycin D) was estimated to be about 24 h irrespective of the hormone treatment, though the half-life was about 6 h in the absence of hormones. These results suggested that the prolongation of the half-life of OVA mRNA by steroid hormones is constant irrespective of differential transcription rates of the OVA gene.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture.
authors.forename: Y
authors.initials: Y
authors.lastname: Arao
authors.forename: N
authors.initials: N
authors.lastname: Miyatake
authors.forename: E
authors.initials: E
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Usiku
authors.forename: A
authors.initials: A
authors.lastname: Matsuura
authors.forename: Y
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: S
authors.initials: S
authors.lastname: Masushige
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D002645:Chickens; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000008:administration & dosage / Q000494:pharmacology; D004338:Drug Combinations; D005786:Gene Expression Regulation / Q000187:drug effects; D006207:Half-Life; D006728:Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D010047:Ovalbumin / Q000096:biosynthesis / Q000235:genetics*; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004338:Drug Combinations; D006728:Hormones; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D013196:Dihydrotestosterone; D011374:Progesterone; D004054:Diethylstilbestrol; D010047:Ovalbumin