Presence of laminin and 67KDa laminin-receptor on endothelial surface of lung capillaries. An immunocytochemical study.
The existence of cell surface-associated molecules has been claimed to play a major role in cellular recognition and interaction. In this respect, different tumor cell lines express laminin and its receptor, and this expression has been correlated with metastatic potential. In the present work, we have studied, by electron microscopic immunolabeling methods, the presence of laminin and 67KDa laminin-receptor on the surface of endothelial cells of lung blood capillaries. To label these molecules, we have developed an easy method in which the labeling is carried out "in situ", in previously excised lungs. The presence of both molecules was observed on the luminal surface of endothelial capillaries and, in many cases, gold particles were associated to small open vesicles of the endothelial cells. The results suggest that these molecules, traditionally associated to extracellular matrix, are also expressed in cellular surface of the lung vascular bed.
Hilario
E
E
Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain.
Unda
F
F
Pérez-Yarza
G
G
Alvarez
A
A
García-Sanz
M
M
Allño
S F
SF
D000818:Animals; D002196:Capillaries; D002462:Cell Membrane / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000648:ultrastructure*; D007797:Laminin / Q000032:analysis*; D008168:Lung / Q000098:blood supply* / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D017474:Receptors, Laminin / Q000032:analysis*
D007797:Laminin; D017474:Receptors, Laminin