Effects of psychotropic drugs on aldo-keto reductase activity in rat ovary and adrenal gland.
We investigated the effects of minor and major tranquilizers on ovarian and adrenal aldo-keto reductase activity towards five substrates in relation to ovulation in mature cycling rats. Nitrazepam (NZP) did not alter ovarian and adrenal weights or body weight, although ovulation was inhibited at 5 and 10 mg/kg. NZP decreased ovarian 13,14-dihydro-15-ketoprostaglandin F2 alpha (15KD-PGF2 alpha) and 4-benzoylpyridine (4BP) reducing activities. None of the doses of zopiclone (ZPC) influenced uterine and adrenal weights or body weight, but it increased ovarian weight at 10 mg/kg. No significant effects of ZPC on ovarian aldo-keto reductase activity were observed. NZP had inhibitory effects on adrenal aldo-keto reductase activity, whereas ZPC had a stimulatory effect. Chlorpromazine (CPZ) did not alter ovarian or adrenal weight, whereas the estrous cycles were abolished at 5 and 10 mg/kg. Reserpine (RSP) decreased ovarian weight and completely inhibited ovulation at 5 and 10 mg/kg, but it increased adrenal weight. Both CPZ and RSP decreased, dose dependently, ovarian aldo-keto reductase activity towards five substrates in agreement with the inhibition of ovulation. On the other hand, differences were found between the effects of CPZ and RSP on adrenal aldo-keto reductase activity. CPZ significantly increased 4BP reducing activity at 5 and 10 mg/kg, although no significant changes were observed in the other four reducing activities. RSP decreased 15KD-PGF2 alpha reducing activity in a dose-dependent manner, whereas the other four activities did not change.
Inazu
N
N
Department of Pharmacology, Teikyo University School of Medicine, Tokyo, Japan.
Hayashi
M
M
Hiura
M
M
Satoh
T
T
D000311:Adrenal Glands / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology*; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000818:Animals; D053961:Azabicyclo Compounds; D002746:Chlorpromazine / Q000633:toxicity; D005260:Female; D009567:Nitrazepam / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology*; D010879:Piperazines / Q000633:toxicity; D011619:Psychotropic Drugs / Q000633:toxicity*; D051381:Rats; D011921:Rats, Inbred WKY; D012110:Reserpine / Q000633:toxicity; D013379:Substrate Specificity; D014149:Tranquilizing Agents / Q000633:toxicity; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects
D053961:Azabicyclo Compounds; D010879:Piperazines; D011619:Psychotropic Drugs; D014149:Tranquilizing Agents; C515050:zopiclone; D012110:Reserpine; D009567:Nitrazepam; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D002746:Chlorpromazine