Determination of optimal liquid medium for enzyme expression by Helicobacter pylori.
OBJECTIVE
To determine the best medium for the growth and enzyme expression of Helicobacter pylori.


METHODS
Twelve H pylori isolates from histologically confirmed infected patients were cultured on Brucella broth (BB), Brucella broth minus bisulphite (BLBB), and brain-heart infusion broth (BHIB), all supplemented with 5% (v/v) fetal calf serum. Growth rates and enzyme expressions of all H pylori isolates cultivated in these media were evaluated.


RESULTS
Although both BLBB and BHIB supported good growth of H pylori under microaerophilic conditions, the total protein content of H pylori detected was much higher with BHIB cultivation. Measurement of the specific activities of urease, phospholipase C, and sphingomyelinase for 12 H pylori isolates cultivated in these media, showed that BHIB supported the highest expression of these enzymes. Although BLBB supported better growth of H pylori than BB, it did not increase enzyme expression.


CONCLUSIONS
Cultivation of H pylori in BHIB is recommended for studies on the physiology, metabolism, and enzyme expression of the organism.
Lin
Y L
YL
Medical Biotechnology Laboratory, School of Medical Technology, Chang Gung College of Medicine and Technology, Kweishan, Taoyuan, Republic of China.
Lee
N
N
Chan
E C
EC
D000818:Animals; D003470:Culture Media; D016480:Helicobacter pylori / Q000201:enzymology* / Q000254:growth & development; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism; D014510:Urease / Q000502:physiology
D003470:Culture Media; D010738:Type C Phospholipases; D013108:Sphingomyelin Phosphodiesterase; D014510:Urease