title: Differential expression of three glutamate transporter subtypes in the rat retina.
abstract: The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype (Rothstein et al. 1994). A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1>/=EAAC-1>GLT-1. GLAST-1 immunoreactivity was seen in Müller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.
authors.affiliation: Max-Planck-Institut für Hirnforschung, Abteilung Neuroanatomie, Deutschordenstrasse 46, D-60528 Frankfurt/M., Germany. rauen@mpih-frankfurt.mgp.de
authors.forename: T
authors.initials: T
authors.lastname: Rauen
authors.forename: J D
authors.initials: JD
authors.lastname: Rothstein
authors.forename: H
authors.initials: H
authors.lastname: Wässle
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001692:Biological Transport; D015153:Blotting, Western; D064032:Calbindin 2; D064026:Calbindins; D002352:Carrier Proteins / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000378:metabolism*; D008297:Male; D051381:Rats; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D064032:Calbindin 2; D064026:Calbindins; D002352:Carrier Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D064030:S100 Calcium Binding Protein G; D002795:Choline O-Acetyltransferase