UVB irradiation upregulation of the Drosophila 1731 retrotransposon LTR requires the same short sequence of U3 region in a human epithelial cell line as in Drosophila cells.
Phylogenetic analysis of the retrotransposon and retrovirus suggests an evolutionary relationship between them and indicates that transactivation of the long terminal repeat (LTR)-containing retroelements could be ubiquitous. Using constructs expressing a reporter gene under the control of the entire or deleted LTR of 1731, which is a retrotransposable element of Drosophila melanogaster, we were able to show that the UVB-irradiation activation of the 1731-LTR requires the same short sequence of U3 region in a human epithelial cell line as in Schneider's Drosophila cell line (S2). This sequence is similar to the binding sequence of the members of the nuclear factor-kappa B (NF-kappa B)/rel family. In addition, human colonic carcinoma cells (HT29), in response to UVB-irradiation, produce some extracellular factor(s) that activates the 1731-LTR in nonirradiated cells.
Faure
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Institut de Chimie Biologique, Université de Provence, Marseille, France.
Best-Belpomme
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D000818:Animals; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects*; D019073:HT29 Cells / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements / Q000235:genetics* / Q000528:radiation effects*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000528:radiation effects*
D018626:Retroelements; D004247:DNA