Immunization with plasmid DNA encoding for the measles virus hemagglutinin and nucleoprotein leads to humoral and cell-mediated immunity.
We have evaluated the DNA vaccination strategy for measles virus (MV) hemagglutinin (HA) and nucleoprotein (NP) genes. Plasmids encoding either the MV, HA, or NP proteins inoculated intramuscularly into Balb/c mice induced both humoral and CTL class I restricted responses. Antibody responses were not increased by multiple inoculations. The major antibody isotype induced by both the HA and NP was IgG2a consistent with a Th1 response. In contrast, immunization with a plasmid which directed the synthesis of a partially secreted form of HA gave mainly IgG1 antibodies. When the amount of DNA was reduced for the HA plasmid (1 or 10 microg/animal), although the antibody was not induced, a CTL response was observed.
Cardoso
A I
AI
INSERM Unit 404 Immunity and Vaccination, Institut Pasteur de Lyon, France.
Blixenkrone-Moller
M
M
Fayolle
J
J
Liu
M
M
Buckland
R
R
Wild
T F
TF
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D005260:Female; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008459:Measles virus / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics* / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines
D000914:Antibodies, Viral; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C032397:hemagglutinin protein G, measles virus; C058314:nucleoprotein, Measles virus