Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus.
Methods for the identification and isolation of environmental isolates of Vibrio vulnificus were evaluated. Alkaline peptone water supplemented with polymyxin B and colistin-polymyxin B-cellobiose agar were employed for the isolation of suspected V. vulnificus from water, sediment and shellfish samples. When comparing the identification of putative V. vulnificus obtained with the API 20E assay and an oligonucleotide probe, 29 API 20E profiles were obtained with only four profiles (representing 20 isolates) reaching the identification threshold of V. vulnificus among a total of 66 isolates hybridizing with the probe. The results indicated that, compared with colony hybridization, the API 20E assay was not adequate for the identification of environmental isolates of V. vulnificus.
Dalsgaard
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Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Dalsgaard
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Høi
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Larsen
J L
JL
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004783:Environmental Microbiology; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics*; D012758:Shellfish / Q000382:microbiology; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014871:Water Microbiology
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes