Blood protein interactions with titanium surfaces.
Protein adsorption and complement activation were studied on thin evaporated films of titanium (Ti). The surfaces were cleaned in either a radio frequency (RF) plasma unit, or washed sequentially in trichloroethylene, acetone, ethanol, and water. Both methods resulted in hydrophilic surface with low carbon contamination on the outermost oxide (approximately 11-13 at%). In situ ellipsometry suggested that Ti is an intrinsic coagulation activator in vitro, since significant amounts of factor XII (F XII) and high molecular weight kininogen (HMWK) were found on the surfaces after 1 min incubation in heparin plasma. Ellipsometry, performed after serum incubations ranging from 15 s to 30 min showed that the total amount of serum proteins and the deposition of antibodies to complement factor 3c (C3c) increased with serum incubation time. ELISA methods showed increased levels of free iC3b in serum after 10 min incubation of the surfaces, but no detectable amounts of C3 convertase fractions C4d or Bb. Ellipsometric results indicated, however, an increased deposition of antibodies to CIq and IgG on Ti after short serum incubation times. The combined results indicate that Ti-surfaces initially activate complement through the classical pathway. The activation then continues via a positive amplification loop where increased amounts of C3 are deposited on the surfaces via the alternative pathway.
Wälivaara
B
B
Linköping University, Department of Physics and Measurement Technology, Sweden.
Askendal
A
A
Lundström
I
I
Tengvall
P
P
D000096:Acetone / Q000737:chemistry; D000327:Adsorption; D000918:Antibody Specificity; D001798:Blood Proteins / Q000378:metabolism*; D003167:Complement Activation / Q000502:physiology*; D003176:Complement C3 / Q000378:metabolism*; D003181:Complement C4 / Q000378:metabolism; D015935:Complement C4b; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000737:chemistry; D005174:Factor XII / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007704:Kininogens / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000378:metabolism*; D014241:Trichloroethylene / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001798:Blood Proteins; D003176:Complement C3; D003181:Complement C4; D007704:Kininogens; D010446:Peptide Fragments; D014867:Water; D000096:Acetone; D014241:Trichloroethylene; D000431:Ethanol; D015935:Complement C4b; C032261:complement C4d; D005174:Factor XII; D014025:Titanium