Genomic structure and chromosomal localization of the human interleukin 15 gene (IL-15).
Interleukin 15 (IL-15) is a novel cytokine whose biological activities are similar to those of interleukin 2 (IL-2). The genomic sequence of human IL-15 has been isolated based on its sequence homology with a cDNA clone encoding human IL-15. The human sequence is 14968 bp in length and includes all six protein-coding exons and five introns. The location of the introns in the human sequence is identical to their positions in the murine IL-15 gene. The same is true for the overall size of the gene, which was estimated to be at least 32 kb. Using Polymerase Chain Reaction (PCR) with gene-specific primers on a panel of human/rodent hybrid cell DNAs, as well as by fluorescence in situ hybridization the human IL-15 gene was mapped to chromosome 4 region q25-35.
Krause
H
H
Institut für Immunologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Germany.
Jandrig
B
B
Wernicke
C
C
Bulfone-Paus
S
S
Pohl
T
T
Diamantstein
T
T
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002894:Chromosomes, Human, Pair 4; D006224:Cricetinae; D018076:DNA, Complementary; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D019409:Interleukin-15 / Q000235:genetics*; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D019409:Interleukin-15