Expression and purification of the crystalline surface layer protein of Rickettsia typhi.
The crystalline surface layer (S-layer) protein (SLP) of Rickettsia typhi is known as the protective antigen against murine typhus. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. To express only the SLP from this gene, the putative signal sequence and the 32-kDa protein portion were removed from the slpT. This protein was expressed in Escherichia coli as a fusion protein, consisting of the SLP and maltose binding protein. The recombinant protein reacted strongly with polyclonal antiserum of a patient with murine typhus.
Hahn
M J
MJ
Department of Microbiology, Kon-Kuk University, Choongju, South Korea.
Chang
W H
WH
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D058619:Maltose-Binding Proteins; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012286:Rickettsia typhi / Q000737:chemistry*
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; C461599:maltose transport system, E coli