title: Expression of membrane form of the pregnancy associated protein TJ6 on decidual lymphocytes in the first trimester of pregnancy.
abstract: TJ6, a newly described protein produced locally in the uterine decidua during pregnancy, may be involved in maintaining a unique immunological environment at the maternal-fetal interface. The aim of this study was to determine whether TJ6 is expressed as membrane form on decidual lymphocytes (DL), to define the phenotypes of TJ6m (membrane form TJ6) expressing cells and to analyze the fluorescence intensity of TJ6m expression. Peripheral blood lymphocytes (PBL) and DL were obtained from first trimester pregnancies undergoing elective termination and immunophenotyped for TJ6m and other cell surface antigens (CD3, CD8, CD19, CD56, CD16) by flow cytometry. This is the first study showing that TJ6 molecules are present on decidual lymphocytes in human pregnancy. TJ6m expression on PBL was not different from that of DL. However, a significantly higher percentage of double positive (TJ6m+CD3+, TJ6m+,CD8+,TJ6m+CD19+) cells were found in PBL when compared to DL. The average fluorescence intensity (AFI) for the TJ6m marker among cells with CD8+, CD19+ and CD56+ double positive was significantly higher in DL as compared with those of PBL. The AFI for granularity of double positive DL was significantly higher than observed in PBL.
authors.affiliation: Department of Physiology and Immunology, University of Rijeka, Croatia.
authors.forename: G
authors.initials: G
authors.lastname: Rubesa
authors.forename: K D
authors.initials: KD
authors.lastname: Beaman
authors.forename: A E
authors.initials: AE
authors.lastname: Beer
authors.forename: H
authors.initials: H
authors.lastname: Haller
authors.forename: D
authors.initials: D
authors.lastname: Rukavina
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D003656:Decidua / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000276:immunology; D011261:Pregnancy Trimester, First / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000276:immunology; D006180:Proton-Translocating ATPases
chemical_list: D015703:Antigens, CD; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C525845:ATP6V0A2 protein, human; D006180:Proton-Translocating ATPases