title: Chromatographic method for the determination of non-transferrin-bound iron suitable for use on the plasma and bronchoalveolar lavage fluid of preterm babies.
abstract: 1. Non-protein-bound iron has been implicated in the aetiology of chronic lung disease of prematurity. 2. The modification of a method for the measurement of non-transferrin-bound iron in small volumes of plasma and bronchoalveolar lavage fluid from preterm babies is described. 3. The assay runs with a good degree of precision and a lower limit of detection of 0.02 mumol/l. 4. Non-transferrin-bound iron was detected in 50% of plasma samples and 11% of bronchoalveolar lavage fluid samples collected over the first week of life from babies born prematurely.
authors.affiliation: University Department of Paediatrics, Sheffield Children's Hospital, U.K.
authors.forename: R
authors.initials: R
authors.lastname: Kime
authors.forename: A
authors.initials: A
authors.lastname: Gibson
authors.forename: W
authors.initials: W
authors.lastname: Yong
authors.forename: R
authors.initials: R
authors.lastname: Hider
authors.forename: H
authors.initials: H
authors.lastname: Powers
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood / Q000378:metabolism*; D007501:Iron / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
chemical_list: D014168:Transferrin; D007501:Iron