Mosquito larvae extract induces mitotic rate alterations of mice tongue keratinocytes along a circadian rhythm period.
It has been demonstrated that the crude extract of mosquito larvae alters the mitotic rate of several mouse cell populations of young growing mice (25 +/- 1 days old). Furthermore, the dialysed fraction of the extract inhibited proliferation of hepatocytes from hepatectomized adult male mice (90 days old). Sampling during the period between 16 and 24 h after treatment (when mitotic peak normally occurs) shows an inhibiting effect on the G1/S interphase, whereas evaluation during the dark phase of the circadian rhythm period (i.e. 4 to 12 h after treatment) shows an increment of the mitotic rates suggesting a probable effect at G2/M restriction point. In the present paper we report the effect of the mosquito larvae crude extract on the proliferative activity of tongue keratinocytes along a circadian rhythm period. Treatments were intraperitoneally applied at 16/00 Time of Day/Time post Injection and mice were killed at 00/08, 04/12, 08/16, 12/20 and 16/24 TD/TPI. As other cell populations previously analysed, the mitotic rate of tongue keratinocytes of extract receivers was significantly increased during night (when S phase normally occurs) and inhibited during the 08/16 to 16/24 period.
Ronderos
J R
JR
Catedra de Histologia y Embriologia 'B', Fac. de Cs. Medicas, Universidad Nacional de La Plata, Argentina.
Riccillo
F L
FL
Ottino
A
A
D000818:Animals; D002940:Circadian Rhythm; D003465:Culex / Q000737:chemistry* / Q000254:growth & development; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007814:Larva; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008938:Mitosis / Q000187:drug effects; D014020:Tissue Extracts / Q000494:pharmacology*; D014059:Tongue / Q000166:cytology
D014020:Tissue Extracts