title: Long-term culture of avian embryonic cells in vitro.
abstract: The pH of the embryonic blood, one of the most important environmental factors for embryonic cells, was found to range from 8.1 to 8.5 in chick embryos until 108 h after incubation. Based on these results, the culture medium adjusted to pH 8.0 was used to culture embryonic chick and quail cells. They were easily subcultured for a long period of time at pH 8.0. This pH culture condition may have wide application for manipulating embryonic cells or tissues and establishing cell lines from avian embryos.
authors.affiliation: Research Development Corporation of Japan, Kumamoto. kuwana@infobears.or.jp
authors.forename: T
authors.initials: T
authors.lastname: Kuwana
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: A
authors.initials: A
authors.lastname: Nakanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Yasuda
authors.forename: A
authors.initials: A
authors.lastname: Tajima
authors.forename: M
authors.initials: M
authors.lastname: Naito
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002470:Cell Survival; D002642:Chick Embryo; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011784:Quail / Q000196:embryology
chemical_list: D003470:Culture Media