Cell cycle control and initiation of DNA replication in Saccharomyces cerevisiae.
The cell cycle of budding yeast is controlled by a single cyclin-dependent protein kinase (Cdc28/Cdk1) which successively associates with the stage-specific activators Cln1-3 (G1 phase), Clb5,6 (S phase), Clb3,4 (S/G2 phase) and Clb1,2 (mitosis). The temporal pattern of these Cdk1/cyclin activities imposes a strict order of DNA replication events during the cycle: formation of a pre-replication complex at late M phase, initiation of replication at the G1/s transition, support of replication during S phase, and the prevention of re-replication at G2. A key component involved in S phase initiation is the Cdc6 protein, a transiently expressed DNA-independent ATP/GTPase interacting at late mitosis with an origin-recognizing complex.
Küntzel
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Max-Planck-Institut für experimentelle Medizin, Göttingen, Germany.
Schulz
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Ehbrecht
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D002453:Cell Cycle / Q000235:genetics*; D004261:DNA Replication; D004789:Enzyme Activation; D011494:Protein Kinases / Q000378:metabolism; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000235:genetics*
D011494:Protein Kinases