Establishment and characterization of a bovine mammary myoepithelial cell line.
The thermolabile large T-antigen, encoded by the simian virus 40 early region mutant tsA58, was used to establish clonal cell lines (BMM-UV) from primary bovine myoepithelial cells. The BMM-UV cells have undergone more than 300 population doublings without any signs of senescence, and they contain the intranuclear large T antigen. At low confluency, they grow in a spindlelike manner and develop very long projections that most likely allow for communication of cells at a distance from each other. Establishment results in a decrease in the number of cells that contract in response to oxytocin compared with the parental nontransfected cells (20% versus 45%). Oxytocin responsiveness of BMM-UV cells increases when the cells are cultured in a medium supplemented with staphylococcal proteases. Proliferation of BMM-UV cells increases when they are cultured in the presence of epidermal growth factor (10 ng/ml) or insulinlike growth factor I (50 ng/ml). The BMM-UV cells may become a useful model to study growth properties, cell-to-cell communication, and the function of bovine mammary myoepithelial cells.
Zavizion
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Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.
van Duffelen
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M
Schaeffer
W
W
Politis
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I
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002417:Cattle; D002455:Cell Division; D002461:Cell Line, Transformed / Q000187:drug effects / Q000648:ultrastructure / Q000821:virology; D002472:Cell Transformation, Viral; D003470:Culture Media / Q000378:metabolism; D004847:Epithelial Cells; D005822:Genetic Vectors; D006133:Growth Substances / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000737:chemistry*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D010121:Oxytocin / Q000494:pharmacology
D000952:Antigens, Polyomavirus Transforming; D003470:Culture Media; D006133:Growth Substances; D010121:Oxytocin