title: Immune response & modulation of immune response induced in the guinea-pigs by Mycobacterium avium complex (MAC) & M. fortuitum complex isolates from different sources in the south Indian BCG trial area.
abstract: A total of 139 guineapigs were used to study the immune response and its modulation induced by Mycobacterium avium complex (MAC) and M. fortuitum complex strains obtained from different sources in the south Indian BCG trial area. The guineapigs were divided into groups and some were directly sensitised/immunised with different MAC strains. M. fortuitum complex strain or BCG and others were sensitised with MAC or M. fortuitum complex and then immunised with BCG. The resulting delayed type hypersensitivity (DTH) response in the different groups of guineapigs was studied by skin tests using PPD-RT23 and PPD-B, and protective response was studied by challenging the guineapigs with a south Indian low virulent strain of M. tuberculosis and enumerating the bacilli in spleen at different points of time. The 3 strains of MAC induced similar low levels of DTH to PPD-RT23 but much higher and varying levels of DTH to PPD-B. MAC strains from soil and sputum induced different levels of immune modulation during subsequent immunisation with BCG on the DTH response to PPD-RT23 and PPD-B. At 2 wk after challenge, 23.8, 81 and 90.5 per cent protection was induced by the standard strain, soil isolate and sputum isolate of MAC, respectively, while 33.3 per cent protection was induced by the M. fortuitum complex strain compared to the protection induced by BCG alone. Prior exposure to MAC or M. fortuitum complex did not have any modulatory effect on the protective immunity due to BCG at this time point. However, at 6 wk after challenge, while the guineapigs immunised with BCG were protected, modulation of the protective response resulting from BCG was observed in the guineapigs sensitised with MAC and M. fortuitum from soil.
authors.affiliation: Tuberculosis Research Centre, Indian Council of Medical Research, Madras.
authors.forename: T
authors.initials: T
authors.lastname: Kamala
authors.forename: C N
authors.initials: CN
authors.lastname: Paramasivan
authors.forename: D
authors.initials: D
authors.lastname: Herbert
authors.forename: P
authors.initials: P
authors.lastname: Venkatesan
authors.forename: R
authors.initials: R
authors.lastname: Prabhakar
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D006968:Hypersensitivity, Delayed; D007194:India; D009165:Mycobacterium Infections, Nontuberculous / Q000276:immunology / Q000517:prevention & control; D015269:Mycobacterium avium Complex / Q000276:immunology* / Q000472:pathogenicity; D015270:Mycobacterium avium-intracellulare Infection / Q000276:immunology / Q000517:prevention & control; D009170:Nontuberculous Mycobacteria / Q000276:immunology* / Q000472:pathogenicity; D012882:Skin Tests; D014611:Vaccination
chemical_list: D001500:BCG Vaccine