Ultrafiltrate sampling device for continuous monitoring.
A light, portable sampling device for the continuous collection and storage of subcutaneous fluid is described. It consists of a hollow-fibre probe for ultrafiltration, a long tube for sample storage and a vacuum tube as driving force. Introduction of a restriction in the flow path allows a constant pulse-free flow as low as 100 nl min-1. The contents of the storage tube reflect the metabolic events that have happened during the 24 h collection period. The ultrafiltrate fluid collected from the subcutaneous tissue is free from large molecules and can be continuously analysed. An application in combination with a bionsensor for the analysis of glucose is described, but the device also allows monitoring of other subcutaneous constituents such as ions, metabolites, drugs and their metabolites.
Moscone
D
D
Department of Biological Psychiatry, University of Groningen, Netherlands.
Venema
K
K
Korf
J
J
D000818:Animals; D015374:Biosensing Techniques; D005947:Glucose / Q000378:metabolism; D008297:Male; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar; D014462:Ultrafiltration / Q000295:instrumentation*
D005947:Glucose