The analysis of fatty acid content and phospholipase A2 activity in placenta of cows with and without retained fetal membranes.
The aim of the following study was the comparison of phospholipase A2 (PLA2) activity and fatty acids content in cows with and without retained fetal membranes. The activity of the enzyme and levels of fatty acids were measured in maternal and fetal placental tissues obtained from cows immediately after spontaneous parturition without (group A) and with (group B) retained fetal membranes. PLA2 activity was statistically significantly (P < 0.05) higher in group A than in group B, and was significantly lower in the fetal part than in the maternal part of placenta in both groups. The content of the examined fatty acids-myristic (14:0), palmitic (16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linolenic (18:3), arachidic (20:0), arachidonic (20:4, AA)-was higher in group A than in B. Concentration of linoleic acid (18:2) showed the opposite relationship. There was a positive correlation between enzyme activity and content of det2ermined fatty acids. The results seem to confirm the bypothesis that the arachidonic acid pathway is altered in cases of retained placenta in cows.
Kankofer
M
M
Institute of Biochemistry Veterinary Medicine Faculty, Agricultural Academy Lublin, Poland.
Wierciñski
J
J
Kêdzierski
W
W
Mierzyñski
R
R
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis / Q000378:metabolism; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D004285:Dogs; D004537:Eicosanoic Acids / Q000032:analysis / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005260:Female; D019814:Myristic Acid; D009227:Myristic Acids / Q000032:analysis / Q000378:metabolism; D019301:Oleic Acid / Q000032:analysis / Q000378:metabolism; D019308:Palmitic Acid / Q000032:analysis / Q000378:metabolism; D010741:Phospholipases A / Q000032:analysis* / Q000378:metabolism; D054467:Phospholipases A2; D010920:Placenta / Q000737:chemistry* / Q000378:metabolism; D011247:Pregnancy; D013229:Stearic Acids / Q000032:analysis / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000032:analysis / Q000378:metabolism
D004537:Eicosanoic Acids; D005227:Fatty Acids; D009227:Myristic Acids; D013229:Stearic Acids; D019814:Myristic Acid; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D010741:Phospholipases A; D054467:Phospholipases A2; C094477:arachidic acid