title: One-step, one-lane chemical DNA sequencing by N-methylformamide in the presence of metal ions.
abstract: We report on a chemical method that allows DNA sequencing by a single reaction. It is based on treatment of 5'-end-labeled DNA with N-methylformamide in the presence of manganese. This method allows the manipulation of samples to be kept to a minimum and consists of a single chemical step that requires about 30 minutes to complete base degradation, phosphodiester bond cleavage and denaturation. Examples of one-treatment, one-lane DNA sequencing of both radioactively and fluorescently 5'-end-labeled DNAs are reported.
authors.affiliation: CNR, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Negri
authors.forename: G
authors.initials: G
authors.lastname: Costanzo
authors.forename: R
authors.initials: R
authors.lastname: Saladino
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
mesh_terms: D005559:Formamides / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology*; D017422:Sequence Analysis, DNA / Q000379:methods*; D015032:Zinc / Q000494:pharmacology
chemical_list: D005559:Formamides; D008345:Manganese; D015032:Zinc; C002950:methylformamide