Molecular cloning of a cDNA encoding enolase from the filamentous fungus, Aspergillus oryzae.
A 1.6-kbp full-length cDNA for the Aspergillus oryzae enolase gene (enoA) was cloned. The sequenced insert contained a continuous open reading frame of 1314 bp encoding a protein of molecular weight 47 405. Among all enolases sequenced to-date, the deduced amino-acid sequence showed the highest homology (74.9%) with Candida albicans enolase (ENO1). Strong codon biases and multiple transcription start sites downstream from CT-blocks in the 5'-flanking region suggested strong expression. enoA mRNA was found to occupy approximately 3% (w/w) of total mRNA of A. oryzae by quantitative RT-PCR. This strong transcription was dependent on the carbon source in the medium and correlated with the growth rate of the mycelium.
Machida
M
M
Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Higashi 1-1, Tsukuba, Ibaraki 305, Japan.
Chang
Y C
YC
Manabe
M
M
Yasukawa
M
M
Kunihiro
S
S
Jigami
Y
Y
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018387:Codon, Initiator; D018076:DNA, Complementary / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018387:Codon, Initiator; D018076:DNA, Complementary; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D002244:Carbon; D010751:Phosphopyruvate Hydratase