title: Nucleotide sequence and functional analysis of the luxE gene encoding acyl-protein synthetase of the lux operon from Photobacterium leiognathi.
abstract: Nucleotide sequence of the luxE gene GenBank Accession No. U66407 from Photobacterium leiognathi PL741 has been determined, and the amino acid sequence of acyl-protein synthetase encoded by the luxE gene is deduced. Nucleotide sequence reveals that the luxE gene encodes acyl-protein synthetase, which is a component of the fatty acid reductase complex that is responsible for converting fatty acid to aldehyde as substrate in the luciferase-catalyzed bioluminescence reaction. The acyl-protein synthetase encoded by the luxE gene has a calculated M, 43,128 and comprises 373 amino acid residues. Alignment and comparison of acyl-protein synthetases from P. leiognathi, P. phosphoreum, Vibrio fischeri, V. harveyi and Xenorhabdus luminescens shows that they are homologous; there is 75.5% homologous (44.2% identity and 31.3% similarity) among these species. Functional analysis illustrates that the specific segment sequence lying before or in the luxE gene might from potential loops omega o omega e1, omega e2 as mRNA stability loop and/or for sub-regulation by alternative modulation in the lux operon. The gene order of the luxE gene in the lux and the lum operons is<--ter-lumQ-lumP-R&R-luxC-luxD-luxA-luxB -luxN-luxE-->(R&R: regulatory region; ter; transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux operons, and ter is the transcriptional terminator for the lum operon.
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China.
authors.forename: J W
authors.initials: JW
authors.lastname: Lin
authors.forename: Y F
authors.initials: YF
authors.lastname: Chao
authors.forename: S F
authors.initials: SF
authors.lastname: Weng
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D010776:Photobacterium / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D000217:Acyltransferases; C034919:acyl protein synthetase