Progress in the development of Lactococcus lactis as a recombinant mucosal vaccine delivery system.
The non-pathogenic, non-colonising Gram-positive organism Lactobacillus lactis is beeing developed as an antigen delivery system for mucosal vaccination. A high level expression system has been developed which allows loading of the bacterium with high levels of a heterologous antigen (TTFC) prior to inoculation. Mucosal inoculation of one such recombinant strain results in a protective serum antibody response and production of TTFC-specific IgA at mucosal sites.
Norton
P M
PM
Department of Pathology, University of Cambridge, UK.
Le Page
R W
RW
Wells
J M
JM
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology*; D009092:Mucous Membrane / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013744:Tetanus Toxin / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C