Isolation and characterization of the Azospirillum brasilense trpE(G) gene, encoding anthranilate synthase.
The Azospirillum brasilense trpE gene has been isolated by DNA hybridization and by genetic complementation of an Escherichia coli trpE deletion mutant. DNA sequence analysis of a 3.1-kb PstI restriction fragment of A. brasilense revealed the presence of an open reading frame encoding a putative TrpE(G) fusion protein. Previously an A. brasilense clone containing trpGDC was identified (Zimmer et al. Mol Gen Genet 229:41-51, 1991). It can, therefore, be concluded that A. brasilense contains two trpG genes. A putative leader peptide is found upstream of trpE(G), containing three consecutive tryptophan residues. Putative terminator and anti-terminator loops have also been identified. The LLESX10S motif, which is responsible for feedback inhibition by tryptophan in other TrpE proteins, is absent in the A. brasilense TrpE(G) fused protein.
De Troch
P
P
F.A. Janssens Laboratory of Genetics, Department of Applied Plant Sciences, Katholieke Universiteit Leuven, Belgium.
Dosselaere
F
F
Keijers
V
V
de Wilde
P
P
Vanderleyden
J
J
D000595:Amino Acid Sequence; D000878:Anthranilate Synthase / Q000235:genetics*; D015806:Azospirillum brasilense / Q000235:genetics*; D003001:Cloning, Molecular; D018628:Gene Dosage; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D019884:Nitrogenous Group Transferases; D009690:Nucleic Acid Conformation; D009876:Operon / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014166:Transferases / Q000235:genetics
D021382:Protein Sorting Signals; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014166:Transferases; D019884:Nitrogenous Group Transferases; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit