Isolation of a cDNA encoding the X enopus homologue of mammalian Cdc25A that can induce meiotic maturation of oocytes.
From a cDNA library of Xenopus laevis (Xl) oocytes, we isolated a cDNA encoding a putative protein phosphatase homologous to mammalian Cdc25A. Sequence analysis predicts that the Xl cdc25A gene product (Xl Cdc25A) consists of 521 amino acid residues and shares overall 55% identity with human Cdc25A. When its mRNA is injected into Xl oocytes, Xl Cdc25A can act as a potent M phase inducer.
Okazaki
K
K
Division of Molecular Genetics, Kurume University, Fukuoka, Japan.
Hayashida
K
K
Iwashita
J
J
Harano
M
M
Furuno
N
N
Sagata
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D009866:Oogenesis / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins; D014982:Xenopus laevis; D020687:cdc25 Phosphatases
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D029867:Xenopus Proteins; D010749:Phosphoprotein Phosphatases; C517008:CDC25B protein, human; C517009:CDC25C protein, human; C102266:Cdc25A protein, Xenopus; D020687:cdc25 Phosphatases