Solid-phase extraction and HPLC determination of levamisole hydrochloride in sheep plasma.
The anthelmintic, levamisole, was determined in sheep plasma by means of ion-pair solid-phase extraction (SPE) and reverse-phase liquid chromatography. The SPE columns were conditioned with 2 ml of methanol followed by 1 ml of octane sulphonic-acid buffer. After sample application, the columns were washed with 2 ml of the same buffer, followed by elution with 90/10 acetonitrile:buffer. A phenyl reverse-phase column (Spherisorb S5 Phenyl, 250 x 4.6 mm) was used with a mobile phase of acetonitrile: 0.005 M of octane sulphonic-acid sodium salt and 0.2% triethylamine in water, pH 3.5, 38/62. Extraction recoveries of 89-94% were achieved over the range from 100-3750 ng/ml. Accuracy and precision were better than 96% and 2.6%, respectively, over said range, with a limit of quantitation of 50 ng/ ml.
Du Preez
J L
JL
Research Institute for Industrial Pharmacy, Potchefstroom University for CHE, South Africa.
Lötter
A P
AP
D000818:Animals; D000969:Antinematodal Agents / Q000008:administration & dosage / Q000032:analysis / Q000097:blood; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods; D007978:Levamisole / Q000008:administration & dosage / Q000032:analysis / Q000097:blood; D012756:Sheep; D013535:Suspensions; D013827:Thiabendazole / Q000008:administration & dosage / Q000032:analysis / Q000097:blood
D000969:Antinematodal Agents; D013535:Suspensions; D007978:Levamisole; D013827:Thiabendazole