Drosophila melanogaster contains both X-linked and autosomal homologues of the gene encoding calcineurin B.
A transcription unit was identified in the 43E polytene band region of the second chromosome of Drosophila melanogaster (Dm) whose putative translation product has 85% amino acid (aa) identity with the B subunit of the calcineurin protein (CnB) from humans. Unlike the previously described intronless Dm CnB gene homologue, which is located within the 4F band region of the X chromosome, the coding region of this second CnB is found to be interrupted by three introns. Conceptual translation of both Dm CnB genes predict proteins of identical size that are 98% identical in aa sequence. Northern blot analyses indicate that Dm pupae and adults express two different CnB-encoding transcripts that are differentially regulated.
Warren
W D
WD
Department of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia. warren@petermac.unimelb.edu.au
Phillips
A M
AM
Howells
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019703:Calcineurin; D002135:Calcium-Binding Proteins / Q000235:genetics*; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics*; D017344:Genes, Insect; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014960:X Chromosome
D002135:Calcium-Binding Proteins; D004247:DNA; D019703:Calcineurin