title: Preservation of the non-rectangular cuticular plate/cell axis angle of outer hair cells.
abstract: Motile properties of outer hair cells (OHCs) may contribute to sharp tuning and amplification in the mammalian cochlea. Shape changes of isolated OHCs in response to various physical and chemical influences have been investigated intensively. However, determinations of shape may have been influenced by unanticipated effects of preparation and preservation of the OHCs investigated. Thus, in a first step, lengths of freshly isolated OHCs from the guinea pig cochlea were determined using a video-enhancing magnification system. The cuticular plate/cell axis angle (CP/CA angle) was then measured in native cells and under the influence of potassium chloride and potassium gluconate incubation. To show the influence of glutaraldehyde (GA) fixation on the isolated OHCs, fixative-dependent changes on cell length and CP/CA angle were recorded in native and preincubated OHCs. In these experiments, the cell length of vital isolated OHCs was between 41.5 micrometers, in the basal turn, and 103.7 micrometers, in the apical turn. The average CP/CA angle was 106 degrees +/- 4.2 degrees (n = 324 cells, turns 1-4) with no statistically significant differences for the four turns. Under the influence of potassium chloride, cell length was reduced by 8.1%. Potassium gluconate incubation led to a shortening of cell length, followed by a 5.3% increase after 5 min. The CP/CA angle under potassium chloride was decreased (97.0 degrees) and was then increased under the influence of potassium gluconate (110.7 degrees) as a result of cuticular plate tilting. Cell shrinkage after fixation depended on the fixative's osmolarity and on the GA concentration. Increased GA levels amplified cell shrinkage from 34% for hypo-osmolar solutions to 15% in iso-osmolar and 29% in hyperosmolar solutions. The CP/CA angle of native and incubated OHCs was not different from those fixed with GA. The present data provide a rational basis for isolated OHC shape parameters. Moreover, functionally induced changes can be better interpreted when OHCs are influenced by fixatives, as shown in the GA experiments.
authors.affiliation: Department of Otolaryngology, Medizinische Hochschule Hannover, Germany.
authors.forename: H G
authors.initials: HG
authors.lastname: Kempf
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.forename: H P
authors.initials: HP
authors.lastname: Zenner
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005942:Gluconates / Q000494:pharmacology; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology* / Q000187:drug effects; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012016:Reference Values; D016707:Tissue Fixation; D014021:Tissue Preservation
chemical_list: D005942:Gluconates; D011189:Potassium Chloride; C030691:gluconic acid; D011188:Potassium