Determination of tallimustine in human plasma by high-performance liquid chromatography.
A sensitive and reproducible HPLC method for the determination of tallimustine (I) in human plasma has been developed and validated. Compound I was extracted from plasma by solid-phase extraction using a C18 cartridge from which the test compound was eluted with a methanol-formic acid mixture. The methanol solution was evaporated to dryness and the residue dissolved in a 0.2 M formic acid in methanol-water (1:1, v/v) mixture, then injected onto the HPLC column. The chromatographic separation was performed isocratically by a reversed-phase column filled with ODS, using a 50 mM KH2PO4-acetonitrile mixture as the mobile phase. The flow-rate was 1 ml/min. The eluate was monitored at 314 nm. No peak interfering with that of I was observed when blank human plasma was assayed. Linearity was established in the concentration range 0.5-85.5 nanograms of I per millilitre of plasma. Four calibration curves in plasma, prepared and run on four different days, showed correlation coefficients higher than 0.99 and good reproducibility of the slope (C.V. = 4.5%). The intra-day precision, evaluated at three concentrations (in the low, mid and high range of the standard curve) and expressed as C.V. ranged from 0.9 to 14.4%. The inter-day precision evaluated at the same concentrations was better than 10.2%. The inter-day accuracy evaluated in the same samples and expressed as the ratio of found/added amount of I, ranged from 86.2 to 108.5%. The limit of quantitation was 0.5 ng/ml plasma. The HPLC method described here was successfully employed for the determination of I in some plasma samples obtained during a phase I clinical trial with the test compound.
Marrari
P
P
Pharmacia, Department of Pharmacokinetics and Metabolism, Milan, Italy.
Pianezzola
E
E
Strolin Benedetti
M
M
D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004214:Distamycins / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D009588:Nitrogen Mustard Compounds / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results
D000970:Antineoplastic Agents; D004214:Distamycins; D007202:Indicators and Reagents; D009588:Nitrogen Mustard Compounds; C066900:tallimustine