title: Identification, cloning, and sequencing of the immunoglobulin A1 protease gene of Streptococcus pneumoniae.
abstract: The pneumococcus expresses a protease that hydrolyzes human immunoglobulin A1 (IgA1). A gene for IgA1 protease was identified from a plasmid library of pneumococcal DNA because of the effect of its overexpression on the colony morphology of Streptococcus pneumoniae. The deduced 1,964-amino-acid sequence is highly homologous to that of the IgA1 protease from Streptococcus sanguis. The similarity to the S. sanguis enzyme and the presence of a putative zinc-binding site suggest that the pneumococcal enzyme is a metalloprotease. The two streptococcal sequences differ in a hydrophilic region with 10 tandem repeats of a 20-mer in S. sanguis, which is replaced by a similar but less repetitive sequence in S. pneumoniae. Antiserum reactive with the pneumococcal IgA1 protease was used to demonstrate that the majority of the protein is cell associated. The expression and function of this gene were confirmed by insertional mutagenesis. Interruption of the chromosomal gene resulted in loss of expression of an approximately 200-kDa protein and complete elimination of detectable IgA1 protease activity.
authors.affiliation: Department of Pediatrics, Children's Hospital of Philadelphia, Pennsylvania, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Wani
authors.forename: J V
authors.initials: JV
authors.lastname: Gilbert
authors.forename: A G
authors.initials: AG
authors.lastname: Plaut
authors.forename: J N
authors.initials: JN
authors.lastname: Weiser
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000235:genetics*
chemical_list: D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase