Purification of bovine beta 2-microglobulin from colostrum and its complete amino acid sequence.
Bovine beta 2-microglobulin (beta 2-m) was purified from colostrum milk in two chromatographic steps: anion-exchange chromatography, and gel filtration. The amino acid sequence was determined to confirm that the purified protein was beta 2-m. A molecular weight of 11.8 kDa beta 2-m was estimated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bovine beta 2-m consists of 98 amino acid residues and contains one disulfide linkage connecting residues 25 and 79. The amino acid sequence determined by this study is different from a previously published sequence at three sites, but agrees with the amino acid sequence from cDNA. Thus, we can conclude that the amino acid sequence determined in this study is correct.
Hoshi
F
F
Department of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Nagai
D
D
Higuchi
S
S
Noso
T
T
Takahashi
A
A
Kawamura
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008969:Molecular Sequence Data; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000302:isolation & purification*
D001613:beta 2-Microglobulin