title: IL-1 beta protein in human skin lymph does not discriminate allergic from irritant contact dermatitis.
abstract: Recent data suggest a key rôle for IL-1 beta in the induction phase of allergic contact dermatitis. In the present study, the protein levels of IL-1 beta were measured in skin lymph derived from normal untreated skin as well as from irritant and allergic (induction and elicitation phase) contact dermatitis. IL-1 beta increased in the course of both types of contact dermatitis, displaying the highest levels in irritant CD. Using a reverse transcriptase-polymerase chain reaction, low signal strength of IL-1 beta mRNA was demonstrated in lymph cells derived from normal skin and allergic CD. In lymph cells collected 2 x daily during the induction phase of allergic contact dermatitis, no upregulation of the IL-1 beta mRNA signal was found. Isolated CD1a+ lymph cells derived from normal skin as well as from the induction and elicitation phase of allergic contact dermatitis did not express IL-1 beta mRNA. Our results demonstrate that in human skin lymph, the IL-1 beta profiles do not discriminate between irritant and allergic contact dermatitis and that besides resident epidermal and dermal cells, circulating lymph cells may also contribute to IL-1 beta protein production.
authors.affiliation: Dermatological Clinic, University of Berne, Switzerland.
authors.forename: C U
authors.initials: CU
authors.lastname: Brand
authors.forename: T
authors.initials: T
authors.lastname: Hunziker
authors.forename: N
authors.initials: N
authors.lastname: Yawalkar
authors.forename: L R
authors.initials: LR
authors.lastname: Braathen
mesh_terms: D000328:Adult; D000485:Allergens; D003521:Cyclopropanes; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D017453:Dermatitis, Irritant / Q000276:immunology*; D004137:Dinitrochlorobenzene; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D007509:Irritants; D007801:Langerhans Cells / Q000276:immunology; D008196:Lymph / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D012867:Skin / Q000276:immunology* / Q000473:pathology; D012967:Sodium Dodecyl Sulfate; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D000485:Allergens; D003521:Cyclopropanes; D004137:Dinitrochlorobenzene; D007375:Interleukin-1; D007509:Irritants; D012333:RNA, Messenger; D012967:Sodium Dodecyl Sulfate; D012194:RNA-Directed DNA Polymerase; C029402:diphenylcyclopropenone