title: Molecular characterization of human zyxin.
abstract: Zyxin is a component of adhesion plaques that has been suggested to perform regulatory functions at these specialized regions of the plasma membrane. Here we describe the isolation and characterization of cDNAs encoding human and mouse zyxin. Both the human and mouse zyxin proteins display a collection of proline-rich sequences as well as three copies of the LIM domain, a zinc finger domain found in many signaling molecules. The human zyxin protein is closely related in sequence to proteins implicated in benign tumorigenesis and steroid receptor binding. Antibodies raised against human zyxin recognize an 84-kDa protein by Western immunoblot analysis. The protein is localized at focal contacts in adherent erythroleukemia cells. By Northern analysis, we show that zyxin is widely expressed in human tissues. The zyxin gene maps to human chromosome 7q32-q36.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: T
authors.initials: T
authors.lastname: Macalma
authors.forename: J
authors.initials: J
authors.lastname: Otte
authors.forename: M E
authors.initials: ME
authors.lastname: Hensler
authors.forename: S M
authors.initials: SM
authors.lastname: Bockholt
authors.forename: H A
authors.initials: HA
authors.lastname: Louis
authors.forename: M
authors.initials: M
authors.lastname: Kalff-Suske
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: D
authors.initials: D
authors.lastname: von der Ahe
authors.forename: M C
authors.initials: MC
authors.lastname: Beckerle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002448:Cell Adhesion; D002874:Chromosome Mapping; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D016335:Zinc Fingers; D060589:Zyxin
chemical_list: D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D008667:Metalloproteins; D012333:RNA, Messenger; C092842:ZYX protein, human; C498285:Zyx protein, mouse; D060589:Zyxin