Genetic speciation of Candida isolates by arbitrarily primed polymerase chain reaction.
Candida species are opportunistic human pathogens capable of causing a variety of clinical diseases. Rapid and precise identification of Candida to species level is essential for effective treatment and management strategies. Conventional diagnosis of candidiasis is sometimes slow and variable. By using a random primer 5'-ACGGGCCAGT-3' in arbitrarily primed polymerase chain reaction, seven common Candida species (i.e., C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. lipolytica, C. tropicalis and C. (Torulopsis) glabrata) produced characteristic DNA band patterns, which enabled rapid determination to species level among them. Further analysis of the nucleotide sequences of Candida specific fragments should improve our understanding of the molecular structures and possible functions of the gene regions involved.
Liu
D
D
Melbourne Pathology, Collingwood, Vic, Australia.
Coloe
S
S
Jones
S L
SL
Baird
R
R
Pedersen
J
J
D002175:Candida / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal