Effect of hypothalamic lesion or chemical axotomy on restitution of immunoreactivity in mice after cyclophosphamide administration.
Splenocytes harvested from mice that underwent chemical axotomy (by 6-hydroxydopamine) or that had electric lesions in their anterior hypothalami demonstrated a significant decrease in their proliferative ability after concanavalin-A stimulation in vitro. In axotomized mice pre-treated with cyclophosphamide, faster restitution of the proliferative activity was observed on days 6-8 after the drug's administration. Splenocytes isolated from mice treated with 6-hydroxydopamine did not exhibit any suppressive activity, which is characteristic of the restitution period following administration of cyclophosphamide. These results indicate that the autonomic nervous system exert a direct effect on immunoreactivity and on processes which are responsible for restitution after cyclophosphamide-induced immunodisorders.
Mazur-Kolecka
B
B
Department of Immunobiology, Polish Academy of Sciences, Cracow, Poland.
Grigoriev
V A
VA
Kubera
M
M
Basta-Kaim
A
A
Shani
J
J
D000818:Animals; D001369:Axons / Q000502:physiology; D003520:Cyclophosphamide / Q000494:pharmacology*; D003714:Denervation; D004558:Electric Stimulation; D007031:Hypothalamus / Q000276:immunology* / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D016627:Oxidopamine / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D016627:Oxidopamine; D003520:Cyclophosphamide