Human brain endothelial cells and astrocytes produce IL-1 beta but not IL-10.
The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-alpha and gamma-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1 beta and beta 2-microglobulin release were also analysed. IL-10 and TNF-alpha mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1 beta and beta 2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-alpha was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.
Corsini
E
E
Istituto Nazionale Neurologico C. Besta, Milan, Italy.
Dufour
A
A
Ciusani
E
E
Gelati
M
M
Frigerio
S
S
Gritti
A
A
Cajola
L
L
Mancardi
G L
GL
Massa
G
G
Salmaggi
A
A
D000328:Adult; D001253:Astrocytes / Q000276:immunology / Q000378:metabolism*; D001921:Brain / Q000098:blood supply* / Q000473:pathology; D002196:Capillaries / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D002532:Intracranial Aneurysm / Q000378:metabolism / Q000473:pathology; D008297:Male; D008577:Meningeal Neoplasms / Q000378:metabolism / Q000473:pathology; D008579:Meningioma / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000235:genetics
D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D001613:beta 2-Microglobulin; D016753:Interleukin-10