Sequence of hepatitis G virus genome isolated from a Japanese patient with non-A-E-hepatitis: amplification and cloning by long reverse transcription-PCR.
The nucleotide sequence of hepatitis G virus (HGV) genome was determined by analysis of cDNA clones obtained by long reverse transcription-polymerase chain reaction (long RT PCR) and 5'- and 3'-rapid amplification of cDNA ends (RACE) from a Japanese patient (Iw) with non A-E hepatitis. The HGV-Iw genome, consisting of 9375 nucleotides, contains a long open reading frame encoding 2873 amino acid residues. Comparison of HGV-Iw with two American isolates of HGV and one African isolate of GB virus C (GBV-C) indicates that although the nucleotide sequences of these isolates were considerably divergent (86.2% to 93.3%), the deduced amino acid sequences shared an extremely high degree of identity (96.1% to 100%). It was also found that HGV-Iw was more closely related to the HGV isolates from USA than to the GBV-C isolate from Africa.
Shao
L
L
Second Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Shinzawa
H
H
Ishikawa
K
K
Zhang
X
X
Ishibashi
M
M
Misawa
H
H
Yamada
N
N
Togashi
H
H
Takahashi
T
T
D003001:Cloning, Molecular; D018076:DNA, Complementary; D018067:Flaviviridae / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D018076:DNA, Complementary; D014764:Viral Proteins