Immunohistochemical localization of a metabotropic glutamate receptor, mGluR7, in ganglion neurons of the rat; with special reference to the presence in glutamatergic ganglion neurons.
Immunoreactivity for the metabotropic glutamate receptor 7 (mGluR7) and that for phosphate-activated glutaminase (PAG) were examined in the trigeminal (TG), dorsal root (DRG), nodose (NG), superior cervical, celiac, and pelvic ganglia of the rat. Virtually all neuronal cell bodies showed mGluR7-like immunoreactivity (mGluR7-LI) in these ganglia. On the other hand, PAG-like immunoreactivity (PAG) was seen in almost all neuronal cell bodies in the TG, DRG and NG, but not in the other ganglia. Co-existence of mGluR7- and PAG-LI in the TG, DRG and NG was confirmed by a double-immunofluorescence immunohistochemical method. The results indicate that virtually all sensory ganglion neurons are glutamatergic and equipped with mGluR7.
Li
J L
JL
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
Ohishi
H
H
Kaneko
T
T
Shigemoto
R
R
Neki
A
A
Nakanishi
S
S
Mizuno
N
N
D000818:Animals; D019085:Fluorescent Antibody Technique, Direct; D005724:Ganglia / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism* / Q000502:physiology*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D010710:Phosphates / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*
D010710:Phosphates; D018094:Receptors, Metabotropic Glutamate; D018698:Glutamic Acid