title: Tetrahymena nuclear proteins that bind to a micronucleus-specific sequence during vegetative growth.
abstract: Tetrahymena thermophila has two nuclei: a micronucleus is transcriptionally silent during vegetative growth and a macronucleus is active. Extensive programmed DNA rearrangement is known to occur during the development of the somatic macronucleus from the germ-line micronucleus. We previously found a 1.4 kb micronucleus-specific sequence, C-element, which was located upstream of the micronuclear calmodulin gene and was eliminated from the macronuclear genome during macronuclear development. Here, using gel mobility shift assays, we show that C-element binding factors, CBFs, are present in the nuclear extract prepared from vegetative cells. Competition experiments demonstrate that CBFs bind to two regions within the C-element. A sequence motif common to these regions is 5'-ATAGATTT-3'.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, lbaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: O
authors.initials: O
authors.lastname: Numata
mesh_terms: D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism; D048631:Micronucleus, Germline / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D016808:Tetrahymena thermophila / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins