Demonstration of slow acetylator genotype of N-acetyltransferase in isoniazid neuropathy using an archival hematoxylin and eosin section of a sural nerve biopsy specimen.
The genotype for N-acetyltransferase was analyzed in five Japanese patients with isoniazid neuropathy by using the allele specific polymerase chain reaction for a single slice of the 30-year-old paraffin-embedded and hematoxylin-eosin stained sural nerve biopsy specimens. We found slow acetylator genotypes for N-acetyltransferase in all isoniazid neuropathy patients. This result confirmed that patients with the slow acetylator genotype tend to develop neuropathy after administration of isoniazid.
Yamamoto
M
M
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
Sobue
G
G
Mukoyama
M
M
Matsuoka
Y
Y
Mitsuma
T
T
D000107:Acetylation / Q000187:drug effects; D000483:Alleles; D000995:Antitubercular Agents / Q000009:adverse effects*; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001706:Biopsy; D017931:DNA Primers / Q000235:genetics; D004801:Eosine Yellowish-(YS); D005838:Genotype; D006416:Hematoxylin; D006801:Humans; D007538:Isoniazid / Q000009:adverse effects*; D009154:Mutation / Q000502:physiology; D016612:Paraffin Embedding; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013497:Sural Nerve / Q000201:enzymology* / Q000503:physiopathology; D013997:Time Factors
D000995:Antitubercular Agents; D017931:DNA Primers; D001191:Arylamine N-Acetyltransferase; D004801:Eosine Yellowish-(YS); D007538:Isoniazid; D006416:Hematoxylin