title: A microbiological assay for the quantitative determination of glutathione.
abstract: Based on the requirement of a glutathione-deficient mutant strain of Saccharomyces cerevisiae to take up external glutathione for growth on synthetic media, a simple agar diffusion test for quantitative detection of total glutathione from various sources was established. Glutathione concentrations can be reliably detected in a less expensive way, requiring less technical effort compared to enzymatic or biochemical detection methods.
authors.affiliation: Johann Wolfgang Goethe-Universität, Frankfurt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Grey
authors.forename: M
authors.initials: M
authors.lastname: Brendel
mesh_terms: D005978:Glutathione / Q000032:analysis*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D005978:Glutathione