title: Flow cytometric analysis of goat milk lymphocytes: subpopulations and adhesion molecule expression.
abstract: Cytometric analysis of cells in milk from healthy goats was achieved after elimination of interfering signals from debris and dead cells by irreversible staining with ethidium monoazide. Some 61% of milk lymphocytes are CD8+ T cells, 17% are CD4+ and about 20% of these express class II antigens: less than 4% are B cells. Compared with blood, the CD4/CD8 ratio was inverted and fewer lymphocytes expressed CD4SR or L-selectin. Monoclonal antibodies to ovine integrins recognised the caprine alpha 4 chain on most lymphocytes from milk or blood, while beta 1 was more frequent on milk cells.
authors.affiliation: Laboratoire Associé de Recherches sur les Lentivirus chez les Petits Ruminants, INRA, Marcy l'Etoile, France.
authors.forename: F
authors.initials: F
authors.lastname: Guiguen
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: E
authors.initials: E
authors.lastname: Pardo
authors.forename: G
authors.initials: G
authors.lastname: Panaye
authors.forename: J F
authors.initials: JF
authors.lastname: Mornex
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D005434:Flow Cytometry; D006041:Goats / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D008892:Milk / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules