Specific interaction of the protein-D2 porin of Pseudomonas aeruginosa with antibiotics.
The protein-D2 porin of Pseudomonas aeruginosa is lacking in carbapenem or fluoroquinolone-resistant strains and hence was thought to facilitate the diffusion of these antibiotics. We examined the effect of several antibiotics on the single channel conductivity of protein-D2 in planar lipid bilayers and found that fluoroquinolones and carbapenems at concentrations of around 1 mM caused closure of the protein-D2 channel. Tetracycline, ampicillin, piperacillin, and latamoxef did not exert any detectable effect on the protein-D2 channel activity.
Ishii
J
J
Department of Molecular Life Science, Tokai University School of Medicine, Japan.
Nakae
T
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004553:Electric Conductivity; D024841:Fluoroquinolones; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008051:Lipid Bilayers; D018272:Porins / Q000187:drug effects* / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D015780:Carbapenems; D024841:Fluoroquinolones; D007473:Ion Channels; D008051:Lipid Bilayers; D018272:Porins