[Effects of disease modifying antirheumatic drugs (DMARDs) and DEX on IL-1 beta and IL-6 production by IL-1 beta stimulated synovial culture cells].
To investigate the effects of disease modifying antirheumatic drugs (DMARDs) and DEX on production of IL-1 beta, IL-6 and TNF-alpha, synovial cells were observed after IL-1 beta administration in vitro.


METHODS
Synovial tissue was obtained aseptically from 8 rheumatoid arthritis patients during joint surgery. The dissected tissue was treated with collagenase and adherent cells were passaged before using as samples. They were stimulated with IL-1 beta (1 ng/ml) and cultured with DMARDs and DEX in serum-free media. After 24 hours' incubation, the production of IL-1 beta, IL-6 and TNF-alpha in the supernatants was measured.


RESULTS
DEX inhibited the production of IL-6. GST inhibited the production of IL-1 beta and IL-6.


CONCLUSIONS
DEX and GST may modulate the disease activity by inhibiting the cytokine production from synovial cells.
Koda
M
M
Department of Joint Diseases, Nippon Medical School First Hospital, Tokyo, Japan.
Yoshino
S
S
Nakamura
H
H
Asano
G
G
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018501:Antirheumatic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D003864:Depression, Chemical; D003907:Dexamethasone / Q000494:pharmacology*; D006052:Gold Sodium Thiomalate / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D008727:Methotrexate / Q000494:pharmacology; D012263:Ribonucleosides / Q000494:pharmacology; D012460:Sulfasalazine / Q000494:pharmacology; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000893:Anti-Inflammatory Agents; D018501:Antirheumatic Agents; D007375:Interleukin-1; D015850:Interleukin-6; D012263:Ribonucleosides; D014409:Tumor Necrosis Factor-alpha; D006052:Gold Sodium Thiomalate; D012460:Sulfasalazine; C010052:mizoribine; D003907:Dexamethasone; D003545:Cysteine; C026535:bucillamine; D008727:Methotrexate