Transformation of the extremely thermoacidophilic archaeon Sulfolobus solfataricus via a self-spreading vector.
We describe a transformation system for extremely thermophilic archaea of the genus Sulfolobus in the kingdom Crenarchaeota. We have constructed in vitro a recombinant derivative of the recently described conjugative plasmid pNOB8, containing a beta-galactosidase gene downstream of a strong promotor. Transformation of a beta-galactosidase negative mutant of Sulfolobus solfataricus with this construct resulted in its spreading through the culture containing the primary transformants and in efficient restoration of beta-galactosidase activity.
Elferink
M G
MG
Max-Planck-Institut für Biochemie, Martinsried, Germany. elferink/vms.bioc
Schleper
C
C
Zillig
W
W
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005822:Genetic Vectors; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000235:genetics; D016944:Sulfolobus / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D012269:Ribosomal Proteins; D001616:beta-Galactosidase