title: ZBP-89, a Krüppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter.
abstract: We have shown previously that a GC-rich element (GGGGCGGGGTGGGGGG) conferring epidermal growth factor (EGF) responsiveness to the human gastrin promoter binds Sp1 and additional undefined complexes. A rat GH4 cell line expression library was screened by using a multimer of the gastrin EGF response element, and three overlapping cDNA clones were identified. The full-length rat cDNA encoded an 89-kDa zinc finger protein (ZBP-89) that was 89% identical to a 49-kDa human factor, ht(beta), that binds a GTGGG/CACCC element in T-cell receptor promoters. The conservation of amino acids between the zinc fingers indicates that ZBP-89 is a member of the C2H2 zinc finger family subclass typified by the Drosophila Krüppel protein. ZBP-89 is ubiquitously expressed in normal adult tissues. It binds specifically to the gastrin EGF response element and inhibits EGF induction of the gastrin promoter. Collectively, these results demonstrate that ZBP-89 functions as a repressor of basal and inducible expression of the gastrin gene.
authors.affiliation: Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA. merchanj@umich.edu
authors.forename: J L
authors.initials: JL
authors.lastname: Merchant
authors.forename: G R
authors.initials: GR
authors.lastname: Iyer
authors.forename: B R
authors.initials: BR
authors.lastname: Taylor
authors.forename: J R
authors.initials: JR
authors.lastname: Kitchen
authors.forename: E R
authors.initials: ER
authors.lastname: Mortensen
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: R J
authors.initials: RJ
authors.lastname: Flintoft
authors.forename: J B
authors.initials: JB
authors.lastname: Michel
authors.forename: R
authors.initials: R
authors.lastname: Bassel-Duby
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D005755:Gastrins; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; D004815:Epidermal Growth Factor