A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp.
A novel PCR amplification method is described which is specific for the thermophilic, enteropathogenic species Campylobacter jejuni, Camp. coli and Camp. upsaliensis. Rapid, accurate speciation of amplified strains is possible on the basis of restriction fragment length polymorphisms of PCR products digested with three restriction enzymes, AluI, DdeI and DraI. The sensitivity of detection is 25 cfu in water, and 2 x 10(3) cfu in full cream milk. An epidemiological application of the assay in detecting non-culturable campylobacters from a contaminated potable water supply is described.
Jackson
C J
CJ
Department of Molecular Biology, Manchester Public Health Laboratory, Withington Hospital, UK.
Fox
A J
AJ
Jones
D M
DM
D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012112:Residential Facilities; D012680:Sensitivity and Specificity; D014871:Water Microbiology; D014881:Water Supply
D004269:DNA, Bacterial