Differential transcription of phycobiliprotein components in Rhodella violacea. Light and nitrogen effects on the 33-kilodalton phycoerythrin rod linker polypeptide, phycocyanin, and phycoerythrin transcripts.
In Rhodella violacea phycoerythrin (PE) has two transcripts, a premessenger and a mature messenger (the gene contains an intron). Phycocyanin, which is plastid-encoded, and the 33-kD PE rod linker polypeptide, which is nuclear-encoded, have only one transcript. The PE premessenger had a rapid turnover; mature transcripts were stable in the light and more stable in the dark. In the presence of rifampicin, cells that shifted from dark to light exhibited an active translation of preexisting transcripts. There are indications of a modulation of the nuclear genome expression by the chloroplast; it may involve an unstable, plastid-encoded translational activator. All transcripts disappeared rapidly during nitrogen starvation. If nitrogen addition was carried out in the dark, active transcription and translation resumed as in light conditions, but ceased after 2 d. Both nitrogen and light were required for a total recovery after nitrogen starvation. Compared with the transcripts of phycobilisome components studied so far in cyanobacteria and Rhodophyceae, the mature transcripts of R. violacea are very stable when nitrogen is not limiting. The unstable PE premessenger is a good indicator of active transcription. This organism is therefore an interesting model to study the regulation of gene expression and the interactions between chloroplastic and nuclear genomes.
Lichtlé
C
C
Laboratoire de Photorégulation et Dynamique des Membranes Végétales, Ecole Normale Supérieure, Centre National de la Recherche Scientifique Unité de Recherche Associée 1810, GDR 1002, Paris, France. lichtle@biologie.ens.fr
Garnier
F
F
Bernard
C
C
Zabulon
G
G
Spilar
A
A
Thomas
J C
JC
Etienne
A L
AL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D045524:Phycobilisomes; D010798:Phycocyanin / Q000096:biosynthesis* / Q000737:chemistry; D010799:Phycoerythrin / Q000096:biosynthesis* / Q000737:chemistry; D010940:Plant Proteins / Q000096:biosynthesis* / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D018087:Plastids / Q000378:metabolism; D014176:Protein Biosynthesis; D012322:RNA Precursors / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D010446:Peptide Fragments; D045524:Phycobilisomes; D010940:Plant Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D010798:Phycocyanin; D010799:Phycoerythrin