Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.
Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsaturated bond of 2-cyclohexen-1-one.
French
C E
CE
Institute of Biotechnology, University of Cambridge, United Kingdom.
Nicklin
S
S
Bruce
N C
NC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003512:Cyclohexanones / Q000378:metabolism; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics*; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D005996:Nitroglycerin / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013739:Testosterone / Q000494:pharmacology
D003512:Cyclohexanones; D011994:Recombinant Proteins; D013739:Testosterone; C028215:2-cyclohexen-1-one; D009249:NADP; D010088:Oxidoreductases; C098939:pentaerythritol tetranitrate reductase; D005996:Nitroglycerin