Determination of alpha-, gamma(+ beta)-, and delta-tocopherols in a variety of liver tissues by reverse-phase high pressure liquid chromatography.
A modified version of a previously published extraction technique using acetone as the extracting medium and separation on an HPLC equipped with a Spherisorb C18 ODS2 column packed with 3 micron particles along with a suitable flow rate and mobile phase significantly improves the resolution of the chromatographic output (fluorescence detector), allowing for accurate measurement of alpha-, delta- and gamma(+ beta)-tocopherols. It was found that two extractions of the same sample yielded approximately 97% of the total extractable alpha-tocopherol. Vitamin C was not needed as an anti-oxidant to protect alpha-tocopherol during extraction from chicken liver but was needed when using fish liver. alpha-tocopherol was found not to be evenly distributed in beef, chicken or fish liver.
Koprivnjak
J F
JF
Environment Canada, Centre Saint-Laurent, Montreal, Québec.
Lum
K R
KR
Sisak
M M
MM
Saborowski
R
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D015203:Reproducibility of Results; D013045:Species Specificity; D014810:Vitamin E / Q000031:analogs & derivatives / Q000032:analysis*
D000975:Antioxidants; D014810:Vitamin E; D001205:Ascorbic Acid