[A laser flow meter method for evaluating the peripheral blood flow in mice].
We have developed a method using a laser flow meter for investigating the effect of compounds on peripheral blood flow. The differences of blood flow in several portions and areas of the probe of a laser flow meter in the mouse and the effect of solvents for dissolving compounds on the blood flow were observed. The results indicated that the invisible area of blood vessels at the back or hind paw of the mouse was the most suitable placement position for the probe. In these placement positions, none of the tested solvents, 0.9% physiological saline solution, dimethylsulfoxide and 50% ethanol, had any significant effect on the peripheral blood flow. Next, using this method, we determined how the peripheral blood flow is affected by several drugs active on the circulatory system. By comparison between the present results from mice and previously reported data from other animals, it was shown that the effects of these cardiovascular agents on the peripheral blood flow in mouse skin showed similar tendencies to those in the previous reports using larger animals and sites other than the skin. However, a few of the drugs used in this experiment showed characteristic action on the peripheral blood flow in mice. Consequently, it was demonstrated that this method using the laser flow meter was useful as a simple first screening method to evaluate the effect of a compound on the peripheral blood flow.
Murayama
M
M
Research Section, Sanwa Shoyaku Co., Ltd., Utsunomiya, Japan.
Miura
T
T
Bando
H
H
D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D012965:Sodium Chloride; D012997:Solvents
D002317:Cardiovascular Agents; D012997:Solvents; D012965:Sodium Chloride; D004121:Dimethyl Sulfoxide