Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry.
A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring with m/z 317 for bromazepam and m/z 270 for internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50-500 ng/g. The lower detection limit in body tissues was 2-5 ng/g and the absolute recovery in body tissues was 27.8-68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.
Zhang
X X
XX
Department of Forensic Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Kudo
K
K
Imamura
T
T
Jitsufuchi
N
N
Nagata
T
T
D014151:Anti-Anxiety Agents / Q000032:analysis* / Q000493:pharmacokinetics; D001923:Brain Chemistry; D001960:Bromazepam / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D012015:Reference Standards; D014018:Tissue Distribution
D014151:Anti-Anxiety Agents; D007202:Indicators and Reagents; D001960:Bromazepam