[Preparation and study of recombinant strains of Venezuelan equine encephalomyelitis virus expressing the PreS2-S gene of the hepatitis B virus].
Venezuelan equine encephalomyelitis (VEE) virus-based vectors for expression of heterologous genes have been constructed using full-length cDNA copy of the interstrain recombinant virus (Trinidad Donkey and 230 strain). Gene cassettes carrying the subgenomic mRNA promoter of VEE virus and the preS2-S gene of hepatitis B virus (HBV) were inserted before or after the genes of structural viral proteins. Live virus stocks were obtained by transfection of chick embryo fibroblasts with in vitro transcribed full-length RNA. Insertions of gene cassettes before the structural region resulted in expression of HBsAg (VEHB-25 and VEHB-361 viruses), whereas insertions in the 3' region did not. Recombinant virus VEHB-25 expressed HBsAg during 5 passages in Vero cells. VEHB-25 stimulated immune response to HBsAg and was less virulent than the parental virus.
Dryga
S A
SA
Sviatchenko
V A
VA
Mikriukova
T P
TP
Fursova
E Iu
EIu
Kiselev
N N
NN
Goncharov
A M
AM
Frolov
I V
IV
Kolykhalov
A A
AA
Agapov
E V
EV
Netesov
S V
SV
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004685:Encephalomyelitis, Venezuelan Equine / Q000235:genetics* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000648:ultrastructure; D006515:Hepatitis B virus / Q000235:genetics*; D008854:Microscopy, Electron; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011817:Rabbits; D011995:Recombination, Genetic; D012692:Serial Passage; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C050807:presurface protein 2, hepatitis B surface antigen