title: High-performance liquid chromatographic determination of vancomycin in rabbit serum, vitreous and aqueous humour after intravitreal injection of the drug.
abstract: A high-performance liquid chromatographic method for the determination of vancomycin in rabbit serum, vitreous and aqueous humour has been developed. No clean-up step was necessary for vitreous and aqueous humour samples. For serum samples liquid-liquid and solid-phase extraction were tested and the best results were achieved using C18 cartridges. The extracts were analyzed on a C18 reversed-phase column, using a mixture of 0.05 M phosphate buffer (pH 4) with 10% of acetonitrile as mobile phase. The detection was carried out at 198 nm, which allows higher sensitivity. The average quantitation limit obtained was 0.03 micrograms/ml. The method has been applied to the study of the residual quantities of vancomycin in serum and rabbit eyes after intravitreal administration of the drug in endophthalmitis treatment.
authors.affiliation: Department of Analytical Chemistry, University de Valladolid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Del Nozal
authors.forename: J L
authors.initials: JL
authors.lastname: Bernal
authors.forename: A
authors.initials: A
authors.lastname: Pampliega
authors.forename: P
authors.initials: P
authors.lastname: Marinero
authors.forename: M I
authors.initials: MI
authors.lastname: López
authors.forename: R
authors.initials: R
authors.lastname: Coco
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D013696:Temperature; D014640:Vancomycin / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin