Association of the putative B-lymphocyte surface molecule B7.3 with a protein kinase activity.
Monoclonal antibody BB1 was previously shown to recognize the CD80 protein as well as a putative B7.3 molecule, both ligands of the important T-cell receptors CD28 and CTLA-4. We report that BB1 coprecipitated from detergent lysates of human B- and pre-B-cell lines a protein kinase activity, as detected by solid phase immunoprecipitation followed by in vitro kinase assay. As other monoclonal antibodies to CD80 did not co-precipitate any kinase activity, it seems likely that this protein kinase is associated with the so far poorly characterized putative B7.3 molecule.
Angelisová
P
P
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha, Czech Republic.
Cerný
J
J
Horejsí
V
V
Eerný
J
J
Hoøejsí
V
V
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D018941:Antigens, CD19 / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D001402:B-Lymphocytes; D018122:B7-1 Antigen / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018941:Antigens, CD19; D000954:Antigens, Surface; D018122:B7-1 Antigen; D011494:Protein Kinases