Determination of dissociation constant and concentration of an anti-DNA antibody by using the light-addressable potentiometric sensor.
The most sensitive assay possible is desired for quantitation of total DNA and DNA probe binding. To reduce background signal, which may reduce assay sensitivity, it is desirable to employ low antibody concentrations. This requires the use of a specific antibody with a high affinity for DNA.
Dill
K
K
Molecular Devices Corporation, Sunnyvale, CA 94089, USA.
Fraser
C
C
Blomdahl
J A
JA
Olson
J D
JD
D000906:Antibodies / Q000378:metabolism*; D000918:Antibody Specificity; D001483:Base Sequence; D001665:Binding Sites; D001666:Binding Sites, Antibody; D004247:DNA / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*; D015342:DNA Probes; D007700:Kinetics; D008027:Light; D008969:Molecular Sequence Data; D011199:Potentiometry / Q000379:methods; D012680:Sensitivity and Specificity
D000906:Antibodies; D015342:DNA Probes; D004247:DNA