Analysis of connexin43 gene expression induced by retinoic acid in F9 teratocarcinoma cells.
All-trans retinoic acid (10(-7) M) induces cell-cell communication and expression of the gap junction protein connexin43 in mouse F9 teratocarcinoma cells. Northern blot analysis revealed an increase of connexin43 mRNA after treatment with retinoic acid, accompanied by an increase of the mRNA of collagen IV, a differentiation marker. To address the question at what level gene expression is enhanced by retinoic acid, nuclear run-on experiments were carried out. There was no detectable change in the level of newly transcribed connexin43 mRNA. Therefore, we postulate a post-transcriptional mechanism responsible for the regulation of connexin43 mRNA levels by retinoic acid.
Clairmont
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A
Institut für Physiologische Chemie I and Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Germany.
Tessmann
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D
Sies
H
H
D000199:Actins / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication / Q000187:drug effects; D003094:Collagen / Q000235:genetics; D018031:Connexin 43 / Q000235:genetics*; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000199:Actins; D018031:Connexin 43; D012333:RNA, Messenger; D014212:Tretinoin; D003094:Collagen