Nucleotide sequence of c-H-ras-1 gene from B6C3F1 mice.
The c-H-ras-1 gene of an B6C3F1 mouse was isolated and nucleotide sequence determined. Our study has revealed that this c-H-ras-1 gene consists of four exons, separated by three introns ranging in size from 150 to 649 bp. The coding parts of the sequence of mouse c-H-ras-1 gene show no important differences as compared with those of the rat, hamster and human gene. More numerous changes were found in introns. The identity of mouse c-H-ras-1 gene with rat, hamster and human ones at the nucleotide level is 86.40%, 80.04% and 67.87%, respectively. Comparison of amino acids in protein sequence of c-H-ras gene of mouse, rat, hamster and human points to high degree of conservation of the gene.
Przybojewska
B
B
Department of Toxicological Biochemistry, Institute of Occupational Medicine, Lódź, Poland.
Płucienniczak
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D011905:Genes, ras; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D004247:DNA