title: Genetic aspects of carbon catabolite repression of the STA2 glucoamylase gene in Saccharomyces cerevisiae.
abstract: Three regulatory genes, known to be required for glucose repression/derepression of some genes in Saccharomyces cerevisiae, were disrupted to study their effects on the carbon-source regulation of the STA2 glucoamylase gene expression. Using a STA2-lacZ fusion it was found that: (1) the MIG1 gene is dispensable for the repression of the STA2 gene; (2) there are two components in the carbon-source repression of STA2: HXK2-dependent and HXK2-independent; and (3) the HAP2 gene seems to be involved in repression rather than activation of the STA2 expression.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Kartasheva
authors.forename: S V
authors.initials: SV
authors.lastname: Kuchin
authors.forename: S V
authors.initials: SV
authors.lastname: Benevolensky
mesh_terms: D000483:Alleles; D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D007763:Lac Operon; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
chemical_list: D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C085976:MIG1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose