Toxicological evaluation of harmful substances by in situ enzymatic and biological detection in high-performance thin-layer chromatography.
The efficiency of a chromatographic analysis method is determined by the selectivity of the chromatographic separation and the specificity of the detection method. In the case of high-performance thin-layer chromatography (HPTLC) the separated components can be detected and quantified directly on the chromatogram by physical and chemical methods. By coupling high-performance thin-layer chromatography with biological or biochemical inhibition tests it was possible to detect toxcologically active substances in situ. A linear relationship was shown between the signal of the inhibition of cholinesterase and the concentration of the inhibitor using a constant enzyme concentration and a constant incubation time. The graph of the inhibition of the luminescence of Photobacterium vibrio fisheri in relation to the concentration of pentachlorophenol (range 20-80 ng) is nearly linear. Measurements were done by using a densitometer or a videodensitometric scanner.
Weins
C
C
Pharmacy and Environmental Technology, University of Saarland, Saarbrücken, Germany.
Jork
H
H
D000818:Animals; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D002802:Cholinesterases / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods; D007306:Insecticides / Q000032:analysis*; D016014:Linear Models; D008163:Luminescent Measurements; D009943:Organophosphorus Compounds; D010776:Photobacterium / Q000737:chemistry; D012680:Sensitivity and Specificity; D014116:Toxicology / Q000379:methods*
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D002802:Cholinesterases