[Expression and significance of PKC alpha in regenerating liver of rats after partial hepatectomy and CCl4 administration].
Immunocytochemistry showed PKC alpha-positive hepatocytes around portal area after two-thirds PH, and they were distributed diffusely in lobules, and reached a peak in number 12 hrs. It's location was not only on membrane facing sinusoid or lateral membrane but also in rough endoplasmic reticulum or in cytosol. Combined technique of immunocytochemistry and ARG revealed presence of (H)-grains at 24 hrs in hepatocytes around the portal tract. PKC alpha was expressed in hepatocytes around central necrosis 6 hrs after administration of CCl4 and its immunoreactivity was visible not only on cytoplasmic membrane but also in cytoplasm, and PKC alpha-positive hepatocytes reached a peak in number at 24 hrs. (H)-labeled hepatocytes were observed around central necrosis at 48 hrs, and AFP was expressed in the same area at 96 hrs. These findings suggest that PKC alpha is expressed in proliferating lesion after PH or administration of CCl4, and that PKC alpha may make an important role in the early phase of cell proliferation, then it is closely associated with liver regeneration.
Ishii
Y
Y
Second Department of Anatomy, Kyoto Prefectural University of Medicine.
D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D006498:Hepatectomy / Q000379:methods; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008107:Liver Diseases / Q000503:physiopathology; D008115:Liver Regeneration; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D007527:Isoenzymes; D002251:Carbon Tetrachloride; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha