Presence of gonadotropin-releasing hormone (GnRH) receptor mRNA in rat myenteric plexus cells.
Idiopathic neuromuscular disease of the gastrointestinal tract (functional bowel disease) is thought to result from the malfunction of neurons within the enteric nervous system. Gonadotropin-releasing hormone (GnRH) analogs have recently been shown to organize the disordered motility patterns typical in these patients and to produce significant, long-term symptomatic improvement. To determine whether GnRH analogs might bind to an endogenous enteric nervous system GnRH receptor, reverse transcription-polymerase chain reaction (RT-PCR) was performed using cultured neonatal rat enteric neuron RNA and rat GnRH receptor primers. A PCR product of the predicted size was cloned and nucleotide sequence analysis demonstrated that the myenteric plexus PCR product encoded a portion of the GnRH receptor sequence previously identified in rat pituitary. These results suggest that cells in the myenteric plexus express GnRH receptors that may bind exogenously administered GnRH analogs. The expression of GnRH receptors in enteric neurons would provide an explanation for the effectiveness of GnRH analogs in treatment of idiopathic neuromuscular disease of the gastrointestinal tract.
Ho
J S
JS
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA.
Nagle
G T
GT
Mathias
J R
JR
Clench
M H
MH
Fan
X
X
Kalmaz
G D
GD
Sallustio
J E
JE
Eaker
E Y
EY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005769:Gastrointestinal Motility / Q000187:drug effects*; D016729:Leuprolide / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009197:Myenteric Plexus / Q000737:chemistry* / Q000166:cytology; D009468:Neuromuscular Diseases / Q000188:drug therapy*; D009474:Neurons / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D011966:Receptors, LHRH / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011966:Receptors, LHRH; D016729:Leuprolide