Differential expression of cysteine-rich intestinal protein in liver and intestine in CCl4-induced inflammation.
Cysteine-rich intestinal protein (CRIP) is a double zinc finger (LIM domain) protein that is developmentally regulated but has an unknown function. CRIP is highly expressed in the intestine, but expression is low in liver. To determine if CRIP expression is regulated under altered physiological status, we used CCl4-induced injury as a model to produce hepatic injury and systemic effects associated with inflammation. Since CRIP is a zinc finger protein and zinc decreases the hepatic response to CCl4, the effect of supplemental dietary zinc (300 mg/kg diet) was also examined. Our results show that this supplemental level of dietary zinc did not affect the index of hepatic injury (plasma alanine aminotransferase), indicating zinc did not have a protective effect. Liver CRIP mRNA increased with CCl4 and CRIP protein was shown by immunohistochemistry to be localized in hepatocytes near the vascular supply. In the intestine, CCl4 caused a transient decrease in CRIP mRNA, but supplemental dietary zinc treatment prevented this decrease. These current results show that CRIP expression changes in response to cellular damage due to acute hepatic injury and are consistent with a functional role for CRIP in proliferation, differentiation, or turnover.
Khoo
C
C
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, USA.
Hallquist
N A
NA
Samuelson
D A
DA
Cousins
R J
RJ
D000818:Animals; D002251:Carbon Tetrachloride; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology; D004032:Diet; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D060588:LIM Domain Proteins; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013997:Time Factors; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology
D002352:Carrier Proteins; C071434:Crip1 protein, rat; D060588:LIM Domain Proteins; D012333:RNA, Messenger; D002251:Carbon Tetrachloride; D015032:Zinc