Mapping of a putative tumor suppressor locus to proximal 7p in Wilms tumors.
Different findings suggest that alterations of chromosome 7 genes play a role in the development of Wilms tumors. To define the positions of these genes, we have accomplished a combined cytogenetic and molecular study on 11 sporadic Wilms tumors. In one case, where both chromosomes 7 were rearranged, the karyotypic picture was consistent with the presence of a tumor suppressor gene at 7p15. To test this hypothesis, a loss of heterozygosity analysis was performed using microsatellite markers. This revealed a common region of allele losses mapped to the proximal short arm of chromosome 7 and defined the position of the gene(s) involved in Wilms tumors within an interval of approximately 25 cM.
Miozzo
M
M
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
Perotti
D
D
Minoletti
F
F
Mondini
P
P
Pilotti
S
S
Luksch
R
R
Fossati-Bellani
F
F
Pierotti
M A
MA
Sozzi
G
G
Radice
P
P
D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D009396:Wilms Tumor / Q000235:genetics*