Continuous cultures of macrophages derived from the 8-day epiblast of the pig.
Secondary macrophage cell cultures were generated from the primary culture of epiblasts of 8-d-old pig blastocysts. The epiblast-derived macrophagelike (EDM) cells have a morphology and ameboid behavior that is typical of tissue histocytes. The cells reacted positively with monoclonal antibodies specific for pig granulocyte-macrophage lineage cells, and were not reactive with monoclonal antibodies specific for pig B and T lymphocytes. Marked phagocytic behavior and the formation of phagosomes were demonstrated following incubation with FITC-labeled bacteria. The EDM cells stained positively for nonspecific acid esterase that was not inhibited by sodium fluoride. DiI-acetylated-LDL was rapidly taken up by the cells. Transmission electron microscopy of the EDM cells showed phagolysosomes, numerous cytoplasmic vacuoles, large, lobed nuclei, and numerous pseudopods or filopodia at the cell surface. Strong reactivity of the cells with anti-CD14 monoclonal antibody was observed. Further, cytotoxic activity was produced from the EDM cells after exposure to lipopolysaccharide in a concentration and time-dependent manner. The cultures could be maintained and expanded for several months on STO co-culture. Their derivation from the epiblast of the pig demonstrates the possibility of obtaining hemopoietic cell cultures from the preimplantation blastocysts of all mammals.
Talbot
N C
NC
USDA, LPSI, ARS, Gene Evaluation and Mapping Laboratory, Beltsville, Maryland 20705, USA.
Worku
M
M
Paape
M J
MJ
Grier
P
P
Rexroad
C E
CE
Pursel
V G
VG
D000107:Acetylation; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002232:Carbocyanines / Q000737:chemistry; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008077:Lipoproteins, LDL / Q000493:pharmacokinetics; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008854:Microscopy, Electron; D009283:Naphthol AS D Esterase / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D002232:Carbocyanines; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D008077:Lipoproteins, LDL; D011971:Receptors, Immunologic; D014409:Tumor Necrosis Factor-alpha; C024286:3,3'-dioctadecylindocarbocyanine; D009283:Naphthol AS D Esterase