[Cloning cDNA for human neurogranin].
By using the human fetal brain library, a 1.2-kb cDNA coding for human neurogranin, a neurospecific endogenous substrate of protein kinase C, was cloned. The results of the Northern blot analysis of the human brain cortex mRNA show that the human neurogranin mRNA exists as a single transcript. Its gene thereby differs from the rat neurogranin gene (RC3), which was shown to produce two mRNAs of different lengths. cDNAs of the human and rat neurogranins show a 70% homology, whereas the protein sequences only differ in three amino acid residues out of the total of 78. The 3'-untranslated region of the human neurogranin cDNA lacks the purine-rich stretch characteristic of the rat neurogranin cDNA.
Mertsalov
I B
IB
Institute of Neurophysiology, Magdeburg, Germany.
Gundelfinger
E
E
Tsetlin
V I
VI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051579:Neurogranin; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; C496649:NRGN protein, human; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D051579:Neurogranin