Comparative study of the determination of bupivacaine in human plasma by gas chromatography-mass spectrometry and high-performance liquid chromatography.
A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.
Tahraoui
A
A
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
Watson
D G
DG
Skellern
G G
GG
Hudson
S A
SA
Petrie
P
P
Faccenda
K
K
D000287:Administration, Topical; D000779:Anesthetics, Local / Q000097:blood*; D002045:Bupivacaine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans
D000779:Anesthetics, Local; D002045:Bupivacaine