CRNF, a molluscan neurotrophic factor that interacts with the p75 neurotrophin receptor.
A 13.1-kilodalton protein, cysteine-rich neurotrophic factor (CRNF), was purified from the mollusk Lymnaea stagnalis by use of a binding assay on the p75 neurotrophin receptor. CRNF bound to p75 with nanomolar affinity but was not similar in sequence to neurotrophins or any other known gene product. CRNF messenger RNA expression was highest in adult foot subepithelial cells; in the central nervous system, expression was regulated by lesion. The factor evoked neurite outgrowth and modulated calcium currents in pedal motor neurons. Thus, CRNF may be involved in target-derived trophic support for motor neurons and could represent the prototype of another family of p75 ligands.
Fainzilber
M
M
Laboratory of Molecular Neurobiology, Department of Neuroscience, Karolinska Institute, Berzelius Laboratories Building, Doktorsringen 12A, S-17177 Stockholm, Sweden. michael@cajal.mbb.ki.se
Smit
A B
AB
Syed
N I
NI
Wildering
W C
WC
Hermann
van der Schors
R C
RC
Jiménez
C
C
Li
K W
KW
van Minnen
J
J
Bulloch
A G
AG
Ibáñez
C F
CF
Geraerts
W P
WP
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D008195:Lymnaea / Q000737:chemistry*; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; C102285:cysteine-rich neurotrophic factor, Lymnaea stagnalis; D002118:Calcium