title: Differential expression of Leishmania major beta-tubulin genes during the acquisition of promastigote infectivity.
abstract: Tubulin expression has been analysed as the insect stage of the protozoan parasite Leishmania major differentiates from a non-infective to an infective form. This transformation of the promastigote stage occurs in vitro and analysis of beta-tubulin mRNA expression in axenically grown promastigotes showed that a 2200 nt transcript is predominately expressed in non-infective promastigotes. The message contains a motif associated with mRNA intracellular localisation and its level is reduced by an order of magnitude in infective promastigotes through a mechanism involving RNA stability. A 3200 nt RNA, the major beta-tubulin transcript in the infective stage, is encoded by a single copy gene at the 3' end of the array that encodes the 2200 nt RNA. These RNAs, as well as a gene encoding a beta-tubulin transcript highly up-regulated in the mammalian stage of the parasite, encode polypeptides that are apparently functionally equivalent but have highly diverged 3' untranslated regions. This differential regulation of the dispersed isogenes may reflect the involvement of a mechanism altering tubulin synthesis during the Leishmania life cycle. The analysis of alpha-tubulin RNA levels revealed the abundance of this message falls as promastigotes differentiate into an infectious stage and the transcript is destabilised in infective promastigotes. These data demonstrate that the regulation of mRNA half-life contributes to controlling gene expression as promastigotes differentiate into an infectious form.
authors.affiliation: Department of Pathology, University of Cambridge, UK. coulson@hgmp.mrc.ac.uk
authors.forename: R M
authors.initials: RM
authors.lastname: Coulson
authors.forename: V
authors.initials: V
authors.lastname: Connor
authors.forename: J C
authors.initials: JC
authors.lastname: Chen
authors.forename: J W
authors.initials: JW
authors.lastname: Ajioka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D018507:Gene Expression Regulation, Developmental; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D018320:Leishmania major / Q000235:genetics* / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D016053:RNA, Protozoan; D014404:Tubulin