Genetic and sequence analysis of the cos region of the temperate Pseudomonas aeruginosa bacteriophage, D3.
The location and structure of the cos ends of bacteriophage D3, which infects Pseudomonas aeruginosa strain PAO, has been determined using a combination of deletion analysis, transposon mutagenesis, and sequencing directly off the phage DNA. Phage D3 was found to have 9-bp 3' cos ends, making it the first phage of a Gram-negative organism known to have 3' cos ends. A 700-bp region flanking the cos site was necessary for efficient transduction of D3 cosmid derivatives. This region was found to contain incomplete inverted repeat sequences flanking the cos site, along with adenine-rich repeats homologous to coliphage gama Ter binding sites. Possible IHF binding sites were also present.
Sharp
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Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Jansons
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Gertman
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Kropinski
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AM
D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D004279:DNA, Viral; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology; D015183:Restriction Mapping; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D003470:Culture Media; D004279:DNA, Viral