title: Adaptation to hypercapnia vs. intracellular pH in cat carotid body: responses in vitro.
abstract: The hypotheses that the chemosensory discharge rate parallels the intracellular pH (pHi) during hypercapnia and that the initial change in pHi (delta pHi) is always more than the stead-state delta pHi were studied by using cat carotid bodies in vitro at 36.5 degrees C in the absence and presence of methazolamide (30-100 mg/l). Incremental acidic hypercapnia was followed by an incremental initial peak response and a greater adaptation. A given acidic hypercapnia elicited a rapid initial response followed by a slower adaptation; isohydric hypercapnia produced an equally rapid initial response but of smaller magnitude that returned to near-baseline level; alkaline hypercapnia induced a similar rapid initial response but one of still smaller magnitude that decreased rapidly to below the baseline. Methazolamide eliminated the initial overshoot, which also suggested involvement of the initial rapid pHi in the overshoot. These results show that the initial delta pHi is always greater than the steady-state delta pHi and during hypercapnia. Also, the steady-state chemoreceptor activity varied linearly with the extracellular pH, indicating a linear relationship between extracellular pH and pHi.
authors.affiliation: Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lahiri
authors.forename: R
authors.initials: R
authors.lastname: Iturriaga
authors.forename: A
authors.initials: A
authors.lastname: Mokashi
authors.forename: F
authors.initials: F
authors.lastname: Botré
authors.forename: D
authors.initials: D
authors.lastname: Chugh
authors.forename: S
authors.initials: S
authors.lastname: Osanai
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D002344:Carotid Body / Q000502:physiology*; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects*; D005260:Female; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D008704:Methazolamide / Q000494:pharmacology
chemical_list: D008704:Methazolamide