title: Regeneration of cut adult axons fails even in the presence of continuous aligned glial pathways.
abstract: The present study tests whether lesions small enough to allow the rapid reestablishment of a normally aligned tract glial framework would provide a permissive environment for the regeneration of cut adult CNS axons. We made penetrating microlesions which cut a narrow beam of axons in the adult rat cingulum, but caused minimal damage to the tract glial framework and no cavitation. The proximal tips of cut axons were identified by enhanced immunoreactivity for low affinity neurotrophin receptor, p75. From 1 day they became expanded into large growth-cone-like structures. At later times some axons turned back and extended in the reverse direction. Up to 14 days (after which time p75 could no longer be used as a marker), no axons advanced beyond the line of the lesion. From 1 to 2 days, OX42 immunostaining and electron microscopy showed that the lesion site was densely infiltrated by macrophages, which disappeared by 3 to 4 days. This was followed by a local hypertrophy of the OX42 immunoreactive resident tract microglial cells and an increase in both GFAP and vimentin immunoreactivity of the tract astrocytes. These responses were greatly reduced by 8 days, when the longitudinal alignment of glial processes across the lesion site was similar to that of an undamaged tract. The large growth-cone-like structures formed at the ends of the cut axons resemble those of developing axons exposed to chemorepulsive factors. This suggests that cellular elements in adult tract lesions may also exert chemorepulsive influences blocking regeneration of axons even in an apparently "open" tract framework.
authors.affiliation: The Division of Neurobiology, National Institute for Medical Research, MRC, London, United Kingdom.
authors.forename: S J
authors.initials: SJ
authors.lastname: Davies
authors.forename: P M
authors.initials: PM
authors.lastname: Field
authors.forename: G
authors.initials: G
authors.lastname: Raisman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D002470:Cell Survival / Q000502:physiology; D003714:Denervation; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000276:immunology; D006179:Gyrus Cinguli / Q000166:cytology; D007150:Immunohistochemistry; D008264:Macrophages / Q000166:cytology; D008751:Methylene Blue; D008854:Microscopy, Electron; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis* / Q000276:immunology; D016622:Silver Staining; D014746:Vimentin / Q000032:analysis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D014746:Vimentin; D008751:Methylene Blue