Molecular cloning of a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans.
We cloned a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans. The nucleotide sequence and Northern blotting indicated that the glutamate transporter gene is transcribed as a polycistronic mRNA in C. elegans and that subsequent trans-splicing yields two distinct monocistronic mRNAs for the glutamate transporter and the ATP synthase c subunit, respectively. The yields of these monocistronic mRNAs were quite different, suggesting that glutamate transport in C. elegans is regulated in the post-transcriptional phase. The glutamate transporter of C. elegans shows about 50-60% sequence similarity with those of mammals. This is the first description of invertebrate glutamate transporters.
Kawano
T
T
Suntory Institute for Bioorganic Research, Osaka, Japan. LDF01673@niftyserve.or.jp
Takuwa
K
K
Nakajima
T
T
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D018076:DNA, Complementary; D005796:Genes; D017238:Genes, Helminth; D008322:Mammals; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010766:Phosphorylation; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D012333:RNA, Messenger; D017868:Cyclic AMP-Dependent Protein Kinases; D006180:Proton-Translocating ATPases