Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.
A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broad host range mob+ plasmids containing gfp expressed from a lac and an npt2 promoter, respectively.
Matthysse
A G
AG
Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
Stretton
S
S
Dandie
C
C
McClure
N C
NC
Goodman
A E
AE
D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins