title: Glycoinositolphospholipids purified from Trypanosoma cruzi stimulate Ig production in vitro.
abstract: We have investigated the effects of glycoinositolphospholipid (GIPL) purified from Trypanosoma cruzi on murine B cell activation. The GIPL neither stimulated any proliferative response by itself, nor affected the blastogenesis induced by surface IgD cross-linking or LPS. On the other hand, the GIPL significantly stimulated both low and high density B cells to secrete IgM in vitro. The GIPL induced B cells to produce IgM when added in the presence of either the surface Ig cross-linker, anti-delta-dextran, or a combination of IL-4 and IL-5. The T. cruzi-derived GIPL also stimulated Ig class switch to IgG1 in cultures stimulated with GIPL, IL-4, and IL-5. The IgG1 secretion was comparable to that induced by LPS plus IL-4. Production of IgG3 was also detected and the GIPL also potentiated the IgG3 production induced by LPS. The stimulatory effect of the T. cruzi-derived GIPL was mediated mainly by its oligosaccharide moiety. This isolated fraction induced a potent IgM secretory response, compared with a much lower response induced by the isolated GIPL ceramide. Taken together, our data suggest that the stimulatory effect of the T. cruzi-derived GIPL on B cell activation could play a role on the conspicuous Ig production observed during infection of the host with T. cruzi.
authors.affiliation: Department of Immunology, Federal University of Rio de Janeiro, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Bento
authors.forename: M B
authors.initials: MB
authors.lastname: Melo
authors.forename: J O
authors.initials: JO
authors.lastname: Previato
authors.forename: L
authors.initials: L
authors.lastname: Mendonça-Previato
authors.forename: L M
authors.initials: LM
authors.lastname: Peçanha
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002240:Carbohydrate Sequence; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D003432:Cross-Linking Reagents; D017261:Glycosylphosphatidylinositols; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015848:Interleukin-5; D015847:Interleukin-4