Met-enkephalin induces cytolytic processes of apoptotic type in K562 human erythroid leukemia cells.
Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, was studied within the 10(-7)-10(-17) M concentration range in K562 human erythroid leukemia cells. Cytolytic activity was determined by the trypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co-incubation with target cells. Discrete maxima of cytolytic activity were detected at concentrations of 10(-9)-10(-10), 10(-13) and 10(-15) M. Cytolysis was accompanied by internucleosomal DNA fragmentation which is indicative of the mechanism of cell death being apoptosis.
Mernenko
O A
OA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Blishchenko
E Y
EY
Mirkina
I I
II
Karelin
A A
AA
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects* / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D015203:Reproducibility of Results; D014343:Trypan Blue; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004744:Enkephalin, Methionine; D014343:Trypan Blue