Antigen-dependent regulation of IgE antibody production by human antigen-specific B cells.
Ag-specific B cells from the peripheral blood of immunized donors were separated following rosette formation with tetanus toxin (TT)-coupled immunobeads to study the regulation of T cell-dependent Ab production. Surface IgM+ (sIgM+) B cells rosetting with TT-coated beads produced anti-TT IgM, IgG, and IgE Abs in cultures stimulated with EBV or anti-CD40 Ab in the presence of IL-4; this was associated with increased numbers of B cells positive for sIgG and sIgE. TT-binding sIgM+ B cells also produced significant amounts of anti-TT IgM, IgG, and IgE Abs when they were cultured with autologous T cells in the presence of IL-4. Addition of TT to the IL-4-stimulated, T cell-dependent cultures resulted in higher levels of TT-specific IgM and IgG Abs than observed with T cells alone, but the inclusion of TT with T cells suppressed anti-TT IgE production. Addition of TT Ag to cultures with allogeneic T cells failed to enhance IgM and IgG responses, and the inhibition of IgE production was reduced compared with cultures containing autologous T cells in the presence of Ag. These results demonstrate that the presence of Ag contributes to the regulation of Ab production, resulting in enhanced IgM and IgG responses, but down-regulation of IgE Ab production.
Oshiba
A
A
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206, USA.
Gelfand
E W
EW
D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D019013:CD40 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000378:metabolism; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D013744:Tetanus Toxin / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D019013:CD40 Antigens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D013744:Tetanus Toxin; D015847:Interleukin-4; D007073:Immunoglobulin E