E2F inhibits transcriptional activation by the retinoic acid receptor.
The E2F transcription factors are thought to mediate growth-inducing signals by elevating transcription of genes required for cell proliferation. Retinoic acid receptors (RARs) mediate retinoic acid (RA)-induced expression of genes with roles in cell differentiation. We found that E2F-1 inhibited expression from RA-responsive promoters. This inhibition was specific to transcription mediated by RARs. We found no direct interaction between the E2F-1 protein and the RA response element in DNA or the RAR proteins. Our evidence suggests that E2F-1 reduces expression from RA-inducible promoters by interacting with an unidentified coactivator(s) that is required by the RARs.
Costa
S L
SL
Ottawa Regional Cancer Centre, University of Ottawa, Canada.
Pratt
M A
MA
McBurney
M W
MW
D000818:Animals; D050882:CREB-Binding Protein; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D014411:Neoplastic Stem Cells; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D047488:Retinoid X Receptors; D015534:Trans-Activators; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation / Q000502:physiology*; D014212:Tretinoin / Q000494:pharmacology
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D009687:Nuclear Proteins; D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D056465:Retinoblastoma-Binding Protein 1; D047488:Retinoid X Receptors; D015534:Trans-Activators; D050680:Transcription Factor DP1; D014157:Transcription Factors; C082925:retinoic acid receptor beta; D014212:Tretinoin; D004247:DNA; D050882:CREB-Binding Protein