Cloning and analysis of a cDNA encoding the BALB/c murine erythrocyte band 7 integral membrane protein.
cDNA clones encoding the BALB/c murine erythrocyte band 7 integral membrane protein (also termed protein 7.2b, or 'stomatin') were isolated by the screening of a corresponding bone-marrow lambda gt11 cDNA library with a human cDNA probe, and by 5'-RACE PCR cloning. Comparison of the murine, human and Caenorhabditis elegans protein 7.2b amino acid (aa) sequences revealed overall identities of 88% (human) and 61% (C. elegans), with the N-terminal domains showing only little similarity. The 7.2b protein sequences of the two mouse strains, BALB/c and C57BL/6J (B6), showed six rather conservative aa substitutions, three of them in the hydrophobic domain. The BALB/c murine mRNA, about 3.5 kb in size, is widely expressed in various tissues, most notably in spleen, lung and testis.
Schlegel
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W
Institute of Biochemistry, University of Vienna, Austria.
Unfried
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I
Prohaska
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R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004910:Erythrocyte Membrane / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D001798:Blood Proteins; D018076:DNA, Complementary; C488964:Stom protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger; C036860:STOM protein, human