title: Novel neural adhesion molecules in the Contactin/F3 subgroup of the immunoglobulin superfamily: isolation and characterization of cDNAs from rat brain.
abstract: We cloned two kinds of cDNAs encoding novel Contactin/F3-subgroup adhesion molecules, which we termed NB-2 and NB-3. Nucleotide sequence analyses have shown that NB-2 and NB-3 are comprised of 1099 and 1028 amino acid residues, respectively. There was 51% similarity in the amino acid sequence of NB-2 and NB-3. NB-2 shared 46, 43, 55 and 55% identities with Contactin/F3, Tag-1, Big-1 and Big-2, respectively, at the amino acid sequence level. Likewise, the amino acid sequence of NB-3 exhibited 42, 44, 58 and 60% identities with Contactin/F3, Tag-1, Big-1 and Big-2, respectively. Expression of NB-2 mRNA was restricted to cerebrum, cerebellum and was hardly detectable, if any, in spinal cord. On the other hand, high expression of NB-3 mRNA was observed in spinal cord, as well as in cerebrum and cerebellum. In the other tissues, no expression of NB-2 and NB-3 mRNAs was detected.
authors.affiliation: Department of Experimental Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Nagata
authors.forename: H
authors.initials: H
authors.lastname: Hosoya
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics*; D002448:Cell Adhesion / Q000235:genetics; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D058969:Contactin 2; D058966:Contactins; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D017261:Glycosylphosphatidylinositols / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; C548676:Cntn2 protein, rat; C088976:Cntn3 protein, rat; C484360:Cntn4 protein, rat; C104187:Cntn5 protein, rat; C104189:Cntn6 protein, rat; D058969:Contactin 2; D058966:Contactins; D018076:DNA, Complementary; D017261:Glycosylphosphatidylinositols; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins