A role for erythrocyte band 3 degradation by the parasite gp76 serine protease in the formation of the parasitophorous vacuole during invasion of erythrocytes by Plasmodium falciparum.
A purified Plasmodium falciparum serine protease (gp76) implicated in erythrocyte invasion, degrades human erythrocyte band 3 and glycophorin A. Inhibition studies using synthetic peptides derived from the presumed band 3 enzymatic cleavage sites and the observed uptake of fluorescent phospholipids following gp76 treatment, suggest that band 3 degradation by this serine protease participates in the formation of the parasitophorous vacuole by restructuring the red cell cytoskeleton. These results provide a rationale for the elaboration of specific inhibitors to block red cell invasion by malaria parasites.
Roggwiller
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Experimental Parasitology, Institut Pasteur, Paris, France.
Bétoulle
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ME
Blisnick
T
T
Braun Breton
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C
D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D002918:Chymotrypsin / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology*; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000502:physiology; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D014617:Vacuoles / Q000378:metabolism / Q000469:parasitology
D001457:Anion Exchange Protein 1, Erythrocyte; D006021:Glycophorins; D008081:Liposomes; D010455:Peptides; D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases; D002918:Chymotrypsin