A mammalian capacitative calcium entry channel homologous to Drosophila TRP and TRPL.
Intracellular Ca2+ signalling evoked by Ca2+ mobilizing agonists, like angiotensin II in the adrenal gland, involves the activation of inositol(1,4,5)trisphosphate(InsP3)-mediated Ca2+ release from internal stores followed by activation of a Ca2+ influx termed capacitative calcium entry. Here we report the amino acid sequence of a functional capacitative Ca2+ entry (CCE) channel that supports inward Ca2+ currents in the range of the cell resting potential. The expressed CCE channel opens upon depletion of Ca2+ stores by InsP3 or thapsigargin, suggesting that the newly identified channel supports the CCE coupled to InsP3 signalling.
Philipp
S
S
Pharmakologisches Institut der Universität Heidelberg, Germany.
Cavalié
A
A
Freichel
M
M
Wissenbach
U
U
Zimmer
S
S
Trost
C
C
Marquart
A
A
Murakami
M
M
Flockerzi
V
V
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins; D027682:Cation Transport Proteins; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004330:Drosophila; D029721:Drosophila Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004594:Electrophysiology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D007295:Inositol Phosphates / Q000494:pharmacology; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels; D019284:Thapsigargin / Q000494:pharmacology; D050051:Transient Receptor Potential Channels
D015220:Calcium Channels; D002148:Calmodulin-Binding Proteins; D027682:Cation Transport Proteins; D029721:Drosophila Proteins; D007295:Inositol Phosphates; D008565:Membrane Proteins; D050052:TRPC Cation Channels; C489571:TRPC4 ion channel; D050051:Transient Receptor Potential Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; C074192:trpl protein, Drosophila; D019284:Thapsigargin; D006160:Guanosine Triphosphate; D002118:Calcium