title: Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.
abstract: Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsaturated bond of 2-cyclohexen-1-one.
authors.affiliation: Institute of Biotechnology, University of Cambridge, United Kingdom.
authors.forename: C E
authors.initials: CE
authors.lastname: French
authors.forename: S
authors.initials: S
authors.lastname: Nicklin
authors.forename: N C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003512:Cyclohexanones / Q000378:metabolism; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics*; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D005996:Nitroglycerin / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013739:Testosterone / Q000494:pharmacology
chemical_list: D003512:Cyclohexanones; D011994:Recombinant Proteins; D013739:Testosterone; C028215:2-cyclohexen-1-one; D009249:NADP; D010088:Oxidoreductases; C098939:pentaerythritol tetranitrate reductase; D005996:Nitroglycerin