title: Interobserver variation in the reporting of cervical colposcopic biopsy specimens: comparison of grading systems.
abstract: OBJECTIVE
  To assess interobserver variation in reporting cervical colposcopic biopsy specimens and to determine whether a modified Bethesda grading system results in better interobserver agreement than the traditional cervical intraepithelial neoplasia (CIN) grading system.
  
  
  METHODS
  One hundred and twenty five consecutive cervical colposcopic biopsy specimens were assessed independently by six histopathologists. Specimens were classified using the traditional CIN grading system as normal, koilocytosis, CIN I, CIN II, or CIN III. The specimens were also classified using a modified Bethesda grading system as either normal, low grade squamous intraepithelial lesion (LSIL) or high grade squamous intraepithelial lesion (HSIL). Participants were also asked to categorise biopsy specimens by the CIN system with the addition of the recently proposed category "basal abnormalities of uncertain significance (BAUS)". The degree of agreement between participants was assessed by kappa statistics.
  
  
  RESULTS
  Using the CIN system, interobserver agreement was generally poor: unweighted and weighted kappa values between individual pairs of observers ranging from 0.05 to 0.34 (average 0.20) and from 0.20 to 0.54 (average 0.36), respectively. With the modified Bethesda system, interobserver agreement was better but still poor: unweighted and weighted kappa values ranging from 0.15 to 0.58 (average 0.30) and from 0.21 to 0.61 (average 0.36), respectively. There was little or no agreement between observers in the diagnosis of BAUS.
  
  
  CONCLUSIONS
  Interobserver agreement in the reporting of cervical colposcopic biopsy specimens using the CIN grading system is poor. Agreement, while still poor, is better when a modified Bethesda grading system is used. There is little or no consensus in the diagnosis of BAUS.
authors.affiliation: Department of Pathology, Royal Group of Hospitals Trust, Belfast.
authors.forename: W G
authors.initials: WG
authors.lastname: McCluggage
authors.forename: H
authors.initials: H
authors.lastname: Bharucha
authors.forename: L M
authors.initials: LM
authors.lastname: Caughley
authors.forename: A
authors.initials: A
authors.lastname: Date
authors.forename: P W
authors.initials: PW
authors.lastname: Hamilton
authors.forename: C M
authors.initials: CM
authors.lastname: Thornton
authors.forename: M Y
authors.initials: MY
authors.lastname: Walsh
mesh_terms: D001706:Biopsy; D003127:Colposcopy; D005260:Female; D006801:Humans; D015588:Observer Variation; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology*