title: Effects on cultured neonatal mouse calvaria of the flavonoids isolated from Boerhaavia repens.
abstract: A MeOH extract from the whole plant of Boerhaavia repens was found to inhibit bone resorption induced by parathyroid hormone (PTH) in tissue culture. Systematic separation of the MeOH extract afforded one new and two known flavonoid glycosides, namely, eupalitin 3-O-beta-D-galactopyranosyl-(1-->2)-beta-D-glucopyranoside (1), eupalitin 3-O-beta-D-galactopyranoside (2), and 6-methoxykaempferol 3-O-beta-D-(1-->6)-robinoside (3). The structure of the new compound 1 was determined using spectroscopic techniques. The inhibitory activity of these substances toward bone resorption induced by PTH was evaluated, and compounds 1 and 2 were found to exhibit significant activity.
authors.affiliation: Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Kadota
authors.forename: T
authors.initials: T
authors.lastname: Namba
authors.forename: T
authors.initials: T
authors.lastname: Miyahara
authors.forename: U G
authors.initials: UG
authors.lastname: Khan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001862:Bone Resorption / Q000139:chemically induced / Q000517:prevention & control; D002132:Calcium Radioisotopes; D046508:Culture Techniques; D004187:Disaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005697:Galactosides / Q000302:isolation & purification / Q000494:pharmacology*; D007564:Japan; D044949:Kaempferols; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D012886:Skull / Q000187:drug effects*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: C103450:6-methoxykaempferol 3-O-rhamnoside; D002132:Calcium Radioisotopes; D004187:Disaccharides; D005419:Flavonoids; D005697:Galactosides; D044949:Kaempferols; D010281:Parathyroid Hormone; D010936:Plant Extracts; C103448:eupalitin 3-O-galactopyranoside; C103449:eupalitin 3-O-galactopyranosyl(1-2)-glucopyranoside