title: Transient expression of CD44 variant isoforms in the ontogeny of the rat: ectoderm-, endoderm- and mesoderm-derived cells express different exon combinations.
abstract: Expression on rat tumor cells of CD44 variant isoforms containing exons v4-v7 or v6-v7 has been described as sufficient for initiation of the metastatic cascade. The question arose as to whether physiological programs may be reactivated by particular CD44 isoforms. With this in mind, expression of mRNAs for the CD44 isoforms was surveyed during ontogeny of the rat. Using available monoclonal antibodies, expression of the CD44 standard isoform (CD44s) and of an epitope of CD44 exon v6 (CD44v6) were evaluated by immunohistology also. While CD44s was expressed in cells of all three germ layers, CD44v6 expression was restricted to distinct epithelial layers and cells of the hematopoietic system. During ontogeny, expression of CD44v6 was first noted in the neural tube and the leading epithelial layer of the limb buds. Later, anti-CD44v6 (1.1ASML) stained basal layers of the epidermis, the epithelium of the gut, and the acini of the submandibular gland. Strong, but transient expression of CD44v6 was seen during lung development, in hematopoietic stem cells of the liver, in thymic epithelia and early thymic immigrants. Expression in these organs was downregulated shortly before or after birth. As revealed by Southern blotting after use of the reverse transcriptase polymerase chain reaction (RT-PCR), most CD44v6-positive organs contained more than one CD44 variant isoform, and the expression patterns differed between individual organs. Hematopoietic cells preferentially expressed exons v4-v7, endodermal tissue exons v4-v10 and only in the epidermis were exons v1-v10 detected. The temporally regulated expression during ontogeny and the different exon compositions suggest a pivotal role of CD44 isoforms particularly in hematopoesis and in pattern formation by instructive epithelia.
authors.affiliation: Germany Cancer Research Center, University of Heidelberg.
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: M
authors.initials: M
authors.lastname: Rösel
authors.forename: R
authors.initials: R
authors.lastname: Arch
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.forename: M
authors.initials: M
authors.lastname: Zöller
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000378:metabolism*; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics*; D007536:Isomerism; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D018076:DNA, Complementary; D018960:Hyaluronan Receptors; D012333:RNA, Messenger