title: Induction of interleukin (IL)-1beta and IL-8 mRNA expression in porcine macrophages by lipopolysaccharide from Serpulina hyodysenteriae.
abstract: Lipopolysaccharide (LPS) is a classic inducer of inflammatory cytokines and is a key virulence factor for most gram-negative pathogens. The effect of phenol-water (LPS) and butanol-water (endotoxin) extracts from Serpulina hyodysenteriae on inflammatory cytokine mRNA expression from porcine alveolar macrophages was investigated. The LPS and endotoxin extracts from S. hyodysenteriae induced a dose-dependent expression of interleukin 1beta (IL-1beta) and IL-8 which was weak compared with the responses induced by Escherichia coli LPS. In addition, the spirochetal extracts induced no detectable upregulation of mRNA expression for either IL-6 or tumor necrosis factor alpha.
authors.affiliation: Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Sacco
authors.forename: S K
authors.initials: SK
authors.lastname: Nibbelink
authors.forename: M J
authors.initials: MJ
authors.lastname: Baarsch
authors.forename: M P
authors.initials: MP
authors.lastname: Murtaugh
authors.forename: M J
authors.initials: MJ
authors.lastname: Wannemuehler
mesh_terms: D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000472:pathogenicity*; D007375:Interleukin-1 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha