Identification, cloning and sequencing the aceA gene involved in acetan biosynthesis in Acetobacter xylinum.
The aceA gene from Acetobacter xylinum was identified and cloned from a genomic DNA library. The complete DNA sequence was determined and computer analysis of the translated gene sequence revealed homology with the deduced amino acid sequence of gumD from Xanthomonas campestris. Therefore aceA is likely to encode the phosphate-prenyl glucose l-phosphate transferase catalyzing the first step in acetan biosynthesis in A. xylinum.
Griffin
A M
AM
Institute of Food Research, Norwich Laboratory, Colney, UK. annette.griffin/b
Morris
V J
VJ
Gasson
M J
MJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D041822:Gluconacetobacter xylinus / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C089843:GumD protein, Xanthomonas campestris; D011135:Polysaccharides, Bacterial; C068545:acetan