[Section plastination of the larynx for histology of whole organ sections].
Whole-organ sections of the larynx have evoked the laryngologists' interest for a long time. Established techniques for histological preparation do exist, but these are time-consuming and result in frequent artifacts. Owing to their thicknesses the slices prepared are usually not suitable for a complete histological work-up. The technique of plastination is an established and gentle method for tissue preservation. We describe the technique of sheet plastination to produce whole-organ sections that allow full-scale histological investigation. Deep-frozen larynges are cut into 4-mm-thick sections, dehydrated in acetone and plastinated in epoxy resins. During plastination, fat and lipids are replaced by curable polymer, making decalcification unnecessary. Specimens are cut into thin sections using a diamond-wire saw. An ultra-milling device reduces the section thickness down to about 10 microns. Staining is possible using a variety of special plastination stains, while all routine stains are also applicable after deplastination. Sheet plastination produces artifact- and shrinkage-free whole organ sections in about 1 week. The technique is useful for studies of micromorphometry or tumor spread and can be used to evaluate quickly possible tumor erosion of the laryngeal skeleton after laryngectomy. Since such erosion is one of the criteria for postoperative radiotherapy in many centers, earlier information could make planning easier.
Sittel
C
C
Universitäts-HNO-Klinik Köln.
Eckel
H E
HE
Sprinzl
G M
GM
Stennert
E
E
D016477:Artifacts; D004396:Coloring Agents; D006652:Histological Techniques / Q000295:instrumentation*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D007830:Larynx / Q000473:pathology*; D009367:Neoplasm Staging; D010969:Plastics; D012016:Reference Values
D004396:Coloring Agents; D010969:Plastics