[Long and accurate PCR (LA PCR)].
PCR (Polymerase Chain Reaction) has become a standard and essential tool used in disciplines such as molecular genetics, genome mapping and sequencing, and forensics. It has proven to be easier, quicker, and less costly than traditional techniques of molecular cloning. While its use in these fielde has already contributed to great advancements and revolutionary changes, current PCR using Taq DNA polymerase is usually limited to amplification up to 5kb. Recently, "long" PCR conditions have been identified which allow the amplification of DNA templates up to 40kb with higher fidelity. In this report, we describe the amplification of longer human genomic fragments (up to 27kb) using LA PCR technology.
Mukai
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Advanced Biomedical Center, Takara Shuzo Co., LTD.
Nakagawa
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D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004259:DNA-Directed DNA Polymerase; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V