Characterization of sodium hypochlorite degradation of beta-glucan in relation to its metabolism in vivo.
Soluble (SSG, beta-1,3-D-glucan obtained from the culture filtrates of a fungus, Sclerotinia sclerotiorum IFO 9395) and particulate (zymosan) beta-glucans were oxidized by sodium hypochlorite (NaClO), and the oxidized products were analyzed by gel filtration and ion-exchange chromatographies and by limulus G-test to study the metabolism of beta-glucans in vivo. By oxidative degradation, SSG was gradually oxidized to anionic polymers, which decreased the molecular weight and reduced the content of the sidechain at the same time. Zymosan, a particle from fungi cell wall, was easily solubilized to a high molecular weight polysaccharide by oxidative degradation. The elution profiles on ion exchange columns and the limulus G-test reactivity of the products were similar to those of polysaccharides obtained by in vivo experiments using SSG and zymosan. These results suggest that oxidative degradation is the main metabolic pathway of beta-glucans in vivo, and that the sidechains would be oxidized faster than the main chain.
Miura
N N
NN
Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Ohno
N
N
Adachi
Y
Y
Yadomae
T
T
D001203:Ascomycota / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004202:Disinfectants / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D008033:Limulus Test; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D012973:Sodium Hypochlorite / Q000737:chemistry*; D015054:Zymosan / Q000737:chemistry; D047071:beta-Glucans
D004202:Disinfectants; D005936:Glucans; D047071:beta-Glucans; D015054:Zymosan; C033363:beta-1,3-glucan; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D010544:Peroxidases