Comparison of the possibilities of gas chromatography-mass spectrometry and tandem mass spectrometry systems for the analysis of anabolics in biological material.
Chromatographic techniques such as GC-MS play a most important role in modern multi-residue analysis of anabolic steroids. The major difference between GC-MS apparatus from different manufacturers is the way of detection and recording. Most apparatus use selected-ion monitoring (SIM) for the determination of low concentrations. Systems based on ion trap technology record in full-scan to even picogram concentrations using a computer algorithm to compare the most important peaks of the mass spectrum of the unknown to those of the standard. In this investigation the possibilities of ion trap GC-MS and the recently released GCQ MS and MS2 for the analysis of anabolics in biological material are compared.
De Brabander
H F
HF
Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
Batjoens
P
P
Courtheyn
D
D
Vercammen
J
J
De Wasch
K
K
D000273:Adipose Tissue / Q000737:chemistry; D045930:Anabolic Agents / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D000818:Animals; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D005243:Feces / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007477:Ions; D007668:Kidney; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis; D009639:Norethandrolone / Q000032:analysis / Q000737:chemistry / Q000652:urine; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000652:urine
D045930:Anabolic Agents; D007477:Ions; D013739:Testosterone; C013915:boldenone; D009639:Norethandrolone