Protective effect of platelet-derived growth factor against 6-hydroxydopamine-induced lesion of rat dopaminergic neurons in culture.
The effects of platelet-derived growth factor (PDGF)-AA and -BB on 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic (DA) neurons were studied in cultures of dissociated fetal rat mesencephalon. The 6-OHDA lesion (0.3% for 4 h) caused a selective 75% loss of tyrosine hydroxylase (TH)-positive DA neurons. The neuronal death was significantly decreased (to about 25%) by addition of 1, 10 or 30 (but not 0.1) ng/ml of PDGF-BB 24 h prior to the insult. In contrast, treatment with PDGF-AA (30 ng/ml) for 24 h before the lesion or addition of PDGF-BB (30 ng/ml) simultaneously with or 1 h after 6-OHDA did not promote the survival of TH-positive neurons. We conclude that PDGF-BB can counteract 6-OHDA-induced degeneration of mesencephalic DA neurons.
Pietz
K
K
Restorative Neurology Unit, Department of Neurology, University Hospital, Sweden. katja.pietz@neurol.lu.se.
Odin
P
P
Funa
K
K
Lindvall
O
O
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004298:Dopamine / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D016627:Oxidopamine / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine