Unraveling diffusion constants in biological tissue by combining Carr-Purcell-Meiboom-Gill imaging and pulsed field gradient NMR.
A diffusion-weighted multi-spin-echo pulse sequence is presented, which allows for simultaneous measurement of T2, the fractional amplitude, and the diffusion constant of different fractions. Monte Carlo simulations demonstrate an improvement of this sequence with respect to the accuracy of diffusion constant and fractional amplitude for slow exchange. Examples are shown for a simple phantom containing two fractions. In addition, experiments on cat brain in healthy condition and following occlusion of the middle cerebral artery show that the fractional amplitude and the diffusion constant of cerebral spinal fluid and normal brain tissue can be analyzed within each pixel with acceptable accuracy.
van Dusschoten
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Agricultural University Wageningen, Department of Molecular Physics, The Netherlands.
Moonen
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CT
de Jager
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Van As
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D000818:Animals; D001921:Brain / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism*; D002415:Cats; D002555:Cerebrospinal Fluid / Q000502:physiology*; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009010:Monte Carlo Method; D019047:Phantoms, Imaging