Conserved TCR usage by HLA-Cw* 1601-restricted T cell clones recognizing melanoma antigens.
In this study we determined TCR alpha and beta chain nucleotide sequences of HLA-Cw* 1601-restricted cytoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the alpha chain CDR3 for two residues and in the beta chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autoiogous melanoma.
Farina
C
C
Division of Experimental Oncology D, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
van der Bruggen
P
P
Boël
P
P
Parmiani
G
G
Sensi
M
M
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D005500:Follow-Up Studies; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D058950:Melanoma-Specific Antigens; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry / Q000276:immunology*
D000951:Antigens, Neoplasm; C092706:BAGE protein, human; D018076:DNA, Complementary; D000939:Epitopes; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta