title: Human papilloma virus DNA: a factor in the pathogenesis of mammary Paget's disease?
abstract: The paraffin sections from 20 nipples with Paget's disease (10 central intraductal and 10 invasive ductal carcinomas) were analyzed for human papilloma virus (HPV) DNA of the low- and intermediate/high-risk groups. Polymerase chain reaction (PCR) and dot (slot) blot hybridization were used for the detection of HPV DNA types 6/11/16/18/31/33/35. In addition, we examined the c-erbB-2 oncogene expression in the specimens to differentiate benign cells in the surface epithelium of the nipple and areola from Paget cells. We found that the oncogene expression of the c-erbB-2 displayed a strong signal in the Paget cells. Using PCR and dot (slot) blot hybridization, we could not detect the HPV DNAs that are specific for the low- and intermediate/high risk-groups in the 20 cases of Paget's disease. Our results showed for the first time that this type of virus did not contribute to the pathogenesis of Paget's disease.
authors.affiliation: Abteilung Gynäkopathologie und Zytologie, Universität Wien, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Czerwenka
authors.forename: F
authors.initials: F
authors.lastname: Heuss
authors.forename: J W
authors.initials: JW
authors.lastname: Hosmann
authors.forename: M
authors.initials: M
authors.lastname: Manavi
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: D
authors.initials: D
authors.lastname: Jelincic
authors.forename: E
authors.initials: E
authors.lastname: Kubista
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D010144:Paget's Disease, Mammary / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases