The kindling-activated neuronal network: recruitment of somatostatin-synthesizing neurons.
The present study demonstrates the anatomical extent of the kindling-activated neuronal network in general, and specifically the recruitment of extrahippocampal somatostatin (SST)-synthesizing neurons into this network. It has been known that SST neurons of the hippocampal formation are activated during episodes of seizure, however, it was not known if this activation was a local event or extended to other areas in the brain. We were therefore interested in determining if and which SST neurons outside the hippocampal formation might be recruited into this seizure-activated neuronal network. Using the kindling model of seizure elicitation, expression of the Fos protein in activated, depolarized neurons was utilized to identify seizure-activated neurons. Subsequently, the mRNA for SST was identified through in situ hybridization in the same tissue section, allowing the identification of seizure-activated, SST-synthesizing neurons. The results show that: (a) the majority of SST-synthesizing neurons in the forebrain and diencephalon became activated during the kindling development; (b) their recruitment into the kindling-activated neuronal network occurred progressively; and, (c) these SST-synthesizing neurons represented a component of the kindling-activated neuronal network throughout the development of kindling-induced seizures.
Pretel
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Department of Neurobiology and Anatomy, University of Rochester, School of Medicine and Dentistry, NY 14642, USA.
Applegate
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Piekut
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D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology; D001923:Brain Chemistry; D004563:Electrochemistry; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011999:Recruitment, Neurophysiological / Q000502:physiology*; D012640:Seizures / Q000503:physiopathology; D013004:Somatostatin / Q000096:biosynthesis*
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D013004:Somatostatin