Purification and characterization of microbial gellan lyase.
Gellan lyase was purified from the culture fluid of soil samples incubated in a medium containing gellan as a sole carbon source. The enzyme was a monomer with a molecular mass of 140 kDa and was most active at pH 7.5 and 45 degrees C. The enzyme was highly specific to gellan and lowered the viscosity of the polymer.
Hashimoto
W
W
Central Research Institute, Maruha Co., Tsukuba City, Japan. hasimoto@food2.food.kyoto-u.ac.jp.
Inose
T
T
Nakajima
H
H
Sato
N
N
Kimura
S
S
Murata
K
K
D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012988:Soil Microbiology; D013379:Substrate Specificity; D014783:Viscosity
D011135:Polysaccharides, Bacterial; C048288:gellan gum; D011133:Polysaccharide-Lyases; C091406:gellan lyase