title: Different expression of adhesion molecules on stromal cells and endothelial cells of capillary hemangioblastoma.
abstract: Cell-cell and cell-matrix interactions are essential for many basic functions, including differentiation and development. In pathological conditions such as inflammation and tumorigenesis adhesive events also play a major role. Cellular adhesion is mediated by specific molecules expressed by both normal and neoplastic tissues. Capillary hemangioblastoma is a tumor of controversial origin, characterized by two major components, vacuolated stromal cells and a capillary network. In order to shed light on the differentiation of the stromal cells and the interactions between the two major components of hemangioblastoma we studied the expression of several adhesion molecules by immunocytochemistry. The endothelium-associated adhesion molecules (ICAM-1, ICAM-2, VCAM-1, PECAM-1 and ELAM-1) were expressed by endothelial cells within the tumors, but not by stromal cells. In contrast, the stromal cells showed strong neuronal cell adhesion molecule (NCAM/CD56) expression, further distinguishing them from endothelial cells. In addition, the stromal cells expressed CD44, which is of interest, as this membrane protein is linked to ezrin, a cytoskeleton-associated protein also expressed by stromal cells. We conclude that the stromal cells and endothelial cells of capillary hemangioblastoma exhibit quite divergent expression patterns of adhesion molecules. The NCAM expression in stromal cells suggests neuroectodermal or mesenchymal differentiation of this tumor. In addition, the NCAM expression could contribute to the sometimes problematic differential diagnosis between capillary hemangioblastoma and metastatic renal cell carcinoma of the central nervous system.
authors.affiliation: Department of Pathology, Haartman Institute, University of Helsinki, Finland. Tom.Bohling@Helsinki.FI
authors.forename: T
authors.initials: T
authors.lastname: Böhling
authors.forename: A
authors.initials: A
authors.lastname: Mäenpää
authors.forename: T
authors.initials: T
authors.lastname: Timonen
authors.forename: L
authors.initials: L
authors.lastname: Vantunen
authors.forename: A
authors.initials: A
authors.lastname: Paetau
authors.forename: M
authors.initials: M
authors.lastname: Haltia
mesh_terms: D002196:Capillaries / Q000378:metabolism; D002528:Cerebellar Neoplasms / Q000473:pathology*; D004727:Endothelium / Q000378:metabolism; D018325:Hemangioblastoma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D019006:Neural Cell Adhesion Molecules; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1