Topically applied human recombinant monoclonal IgG1 antibody and its Fab and F(ab')2 fragments protect mice from vaginal transmission of HSV-2.
A recombinant human anti-herpes simplex virus monoclonal IgG1, antibody and the corresponding Fab and F(ab')2 fragments were tested for efficacy in preventing vaginal transmission of HSV-2 infection in a well-established mouse model for genital herpes. IgG1, Fab, and F(ab')2 were approximately equally protective; vaginal delivery of 1-5 ng provided approximately 50% protection, and vaginal delivery of 400 ng completely protected mice from genital herpes infection (P < 0.001). These results suggest that topical applications of human monoclonal antibodies may be useful in developing new methods for preventing sexually transmitted disease.
Zeitlin
L
L
Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Whaley
K J
KJ
Sanna
P P
PP
Moench
T R
TR
Bastidas
R
R
De Logu
A
A
Williamson
R A
RA
Burton
D R
DR
Cone
R A
RA
D000282:Administration, Intravaginal; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000914:Antibodies, Viral / Q000008:administration & dosage*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D005347:Fibroblasts; D006558:Herpes Genitalis / Q000517:prevention & control* / Q000635:transmission; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000008:administration & dosage*; D007074:Immunoglobulin G / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000008:administration & dosage; D014621:Vagina / Q000821:virology; D017201:Virus Shedding
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011994:Recombinant Proteins