Imaging red blood cells with the atomic force microscope.
A novel technique for the reproduction of ultramorphological images and details of the surface of normal and pathological red blood cells (RBC) was investigated. The atomic force microscope (AFM) provided high-resolution images of cell surfaces. The RBC dimensions obtained by this technique revealed differences between native red cells in smears and glutaraldehyde-fixed red cells. It was shown that fixed red blood cells were best suited for the ultramorphological imaging of the cell surface.
Zachée
P
P
CRUH, Clinical Research Unit Haematology, Katholieke Universiteit Leuven, Belgium.
Snauwaert
J
J
Vandenberghe
P
P
Hellemans
L
L
Boogaerts
M
M
D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*