A cryptic plasmid pBL1 from Brevibacterium lactofermentum causes growth inhibition and filamentation in Escherichia coli.
A shuttle vector composed of pBL1, a 4.46-kb cryptic plasmid from coryneform bacterium Brevibacterium lactofermentum, and Escherichia coli vector pHSG298 was found to inhibit growth and cause cell filamentation in E coli. After construction of several deletions of pBL1, a 1.23-kb AccI-HindIII fragment was found responsible. DNA sequence analysis showed that this fragment contained a 726-bp-long open reading frame (ORF3), encoding a protein with 242 amino acid residues. Corresponding to ORF3, a 28-kDa protein was detected in an in vitro protein synthesis system. ORF3 was dispensable for the stable maintenance of pBL1 in coryneform bacteria.
Goyal
D
D
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Wachi
M
M
Kijima
N
N
Kobayashi
M
M
Yukawa
H
H
Nagai
K
K
D001951:Brevibacterium / Q000235:genetics* / Q000502:physiology; D002455:Cell Division / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames / Q000502:physiology; D010957:Plasmids / Q000502:physiology*