Molecular epidemiology provides evidence of genotypic heterogeneity of multidrug-resistant Pseudomonas aeruginosa serotype O:12 outbreak isolates from a pediatric hospital.
Ribotyping randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis were used for the epidemiologic evaluation of eight Pseudomonas aeruginosa O:12 isolates obtained from eight children and two P. aeruginosa O:12 environmental isolates from a hematology ward. Randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis were able to discriminate isolates that were indistinguishable by biochemical typing, O serotyping or ribotyping.
Bingen
E
E
Service de Microbiologie, Hôpital Robert Debré, Paris, France.
Bonacorsi
S
S
Rohrlich
P
P
Duval
M
M
Lhopital
S
S
Brahimi
N
N
Vilmer
E
E
Goering
R V
RV
D000293:Adolescent; D001483:Base Sequence; D002648:Child; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D018432:Drug Resistance, Multiple; D005260:Female; D005602:France / Q000453:epidemiology; D005838:Genotype; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D010641:Phenotype; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping
D017931:DNA Primers; D004269:DNA, Bacterial