Determination of raffinose and lactobionic acid in ViaSpan by anion exchange chromatography with pulsed amperometric detection.
An anion exchange chromatographic method has been developed to quantify raffinose and lactobionic acid in ViaSpan, an organ preservation product. Separation was accomplished using an aqueous sodium hydroxide/acetate solvent system (pH 13) on a Carbopac PA1 column. Detection was performed using a pulsed amperometric detector equipped with a gold working electrode. The method was able to resolve raffinose,lactobionic acid and other ingredients in the ViaSpan product. Validation testing of the method for routine use produced excellent linearity, precision and accuracy. The limits of detection for raffinose and lactobionic acid were 1.0 ng (1.7 x 10(-12) mol, S/N = 3) and 2.0 ng (5.6 x 10(-12) mol, S/N = 3) respectively. The total analysis time is less than 15 min.
Sigvardson
K W
KW
DuPont Merck Pharmaceutical Company, Wilmington, DE 19880-0353, USA.
Eliason
M S
MS
Herbranson
D E
DE
D000241:Adenosine / Q000032:analysis; D000493:Allopurinol / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods; D004187:Disaccharides / Q000032:analysis*; D005978:Glutathione / Q000032:analysis; D007328:Insulin / Q000032:analysis; D019188:Organ Preservation Solutions; D011887:Raffinose / Q000032:analysis*; D014021:Tissue Preservation
D004187:Disaccharides; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; C005608:lactobionic acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose