title: Inhibition of aminopeptidase A activity causes an acute albuminuria in mice: an angiotensin II-mediated effect?
abstract: The hydrolase aminopeptidase A is an important regulator of the renin-angiotensin system, since it inactivates its most vasoactive component angiotensin II (Ang II). A single i.v. injection of a monoclonal antibody against mouse aminopeptidase A (ASD-4) induces a membranous-like glomerulonephritis in mice, characterized by an acute albuminuria, that is not dependent on complement, the coagulation system, or inflammatory cells. We hypothesized that this albuminuria is the consequence of a reduction in aminopeptidase A enzyme activity, that might subsequently lead to an increase in Ang II levels. Aminopeptidase A enzyme activity was analysed in vitro by a fluorimetric enzyme assay and in vivo by enzyme histochemistry. The role of Ang II in the induction of albuminuria in this model was studied by measuring the renal aminopeptidase A mRNA expression in our model by a competitive PCR assay as an indirect measure of Ang II levels. In addition, the role of Ang II in this model was studied by preventing the formation of Ang II with the angiotensin-converting enzyme inhibitor enalapril or by blocking of the Ang II receptor with the AT1 receptor antagonist losartan. Only antibodies that were able to inhibit the aminopeptidase A enzyme activity in vitro and in vivo induced an acute albuminuria in mice. Renal aminopeptidase A mRNA expression was increased by injection of the anti-aminopeptidase A antibody. Both enalapril and losartan treatment reduced the acute albuminuria, measured 1 day after injection of a monoclonal antibody against aminopeptidase A, by 91% and 83%, respectively. It is concluded that the induction of acute albuminuria is correlated to the enzyme-inhibiting capacity of the anti-aminopeptidase A antibodies. This impaired enzymatic activity most likely leads to an increase in the levels of Ang II, the best known substrate of aminopeptidase A. The results of our additional experiments are in keeping with our hypothesis that Ang II mediates this acute albuminuria. Whether this occurs by an increase of blood pressure or by a growth factor-like effect remains to be defined by further studies in this model.
authors.affiliation: Department of Pathology, University Hospital Nijmegen, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Mentzel
authors.forename: K J
authors.initials: KJ
authors.lastname: Assmann
authors.forename: H B
authors.initials: HB
authors.lastname: Dijkman
authors.forename: A S
authors.initials: AS
authors.lastname: de Jong
authors.forename: J P
authors.initials: JP
authors.lastname: van Son
authors.forename: J F
authors.initials: JF
authors.lastname: Wetzels
authors.forename: R A
authors.initials: RA
authors.lastname: Koene
mesh_terms: D000208:Acute Disease; D000419:Albuminuria / Q000209:etiology* / Q000378:metabolism; D000626:Aminopeptidases / Q000032:analysis / Q000037:antagonists & inhibitors*; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D004656:Enalapril / Q000494:pharmacology; D043384:Glutamyl Aminopeptidase; D006651:Histocytochemistry; D007093:Imidazoles / Q000494:pharmacology; D017403:In Situ Hybridization; D019808:Losartan; D051379:Mice; D008807:Mice, Inbred BALB C; D013777:Tetrazoles / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D001713:Biphenyl Compounds; D007093:Imidazoles; D013777:Tetrazoles; D000804:Angiotensin II; D004656:Enalapril; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase; D019808:Losartan