Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.
An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.
Hirayama
H
H
Deep Star Group, Japan Marine Science and Technology Center, Yokosuka, Kanagawa.
Tamaoka
J
J
Horikoshi
K
K
D000327:Adsorption; D000818:Animals; D001710:Biotin / Q000378:metabolism; D003016:Clostridium perfringens / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D005470:Fluorometry; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D012474:Salmon; D012997:Solvents / Q000494:pharmacology; D013094:Spermatozoa / Q000737:chemistry
D012997:Solvents; D001710:Biotin; D004247:DNA