A platelet aggregation inhibitor phospholipase A2 from Russell's viper (Vipera russelli) venom: isolation and characterization.
Vipera russelli venom is reported to contain multiple forms of phospholipase A2 (PLA2). Three PLA2s (VRV-PL-V, VRV-PL-VI and VRV-PL-VIIIa) have been purified and characterized in this laboratory. A PLA2(VRV-PL-IIIb) inhibiting platelet aggregation was purified from the same venom in two steps involving CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV-PL-IIIb is a basic (pI 7.3-7.7) isoenzyme, with a mol. wt between 14,000 and 15,000. It induced neurotoxic symptoms in experimental mice with an i.p. LD50 of 5.2 mg/kg body weight. VRV-PL-IIIb inhibits ADP-induced platelet aggregation in a dose-dependent manner. It induced oedema in the foot pads of mice and is devoid of anticoagulant, myotoxic and direct haemolytic activities. Antibodies to VRV-PL-IIIb showed a single precipitin line against the antigen as well as whole venom.
Prasad
B N
BN
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Kemparaju
K
K
Bhatt
K G
KG
Gowda
T V
TV
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects; D004487:Edema / Q000139:chemically induced; D006801:Humans; D007194:India; D007928:Lethal Dose 50; D051379:Mice; D010741:Phospholipases A / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity*; D054467:Phospholipases A2; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000633:toxicity*; D017840:Daboia; D014757:Viper Venoms / Q000201:enzymology* / Q000276:immunology
D000906:Antibodies; D010975:Platelet Aggregation Inhibitors; D014757:Viper Venoms; D010741:Phospholipases A; D054467:Phospholipases A2