Transient uptake and storage of serotonin in developing thalamic neurons.
Serotonin (5-HT) has been shown to affect the development and patterning of the mouse barrelfield. We show that the dense transient 5-HT innervation of the somatosensory, visual, and auditory cortices originates in the thalamus rather than in the raphe: 5-HT is detected in thalamocortical fibers and most 5-HT cortical labeling disappears after thalamic lesions. Thalamic neurons do not synthesize 5-HT but take up exogenous 5-HT through 5-HT high affinity uptake sites located on thalamocortical axons and terminals. 3H-5-HT injected into the cortex is retrogradely transported to thalamic neurons. In situ hybridization shows a transient expression of the genes encoding the serotonin transporter and the vesicular monoamine transporter in thalamic sensory neurons. In these glutamatergic neurons, internalized 5-HT might thus be stored and used as a "borrowed transmitter" for extraneuronal signaling or could exert an intraneuronal control on thalamic maturation.
Lebrand
C
C
INSERM U106, IFR des Neurosciences, Hôpital de la Salpêtrière, Paris,France.
Cases
O
O
Adelbrecht
C
C
Doye
A
A
Alvarez
C
C
El Mestikawy
S
S
Seif
I
I
Gaspar
P
P
D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins; D009475:Neurons, Afferent / Q000737:chemistry / Q000378:metabolism*; D009479:Neuropeptides; D018377:Neurotransmitter Agents / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000096:biosynthesis / Q000276:immunology / Q000493:pharmacokinetics*; D050486:Serotonin Plasma Membrane Transport Proteins; D013572:Synaptic Vesicles / Q000378:metabolism; D013788:Thalamus / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; C493232:Slc6a4 protein, rat; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins; D014316:Tritium; D012701:Serotonin