title: Quantitative determination of norethisterone acetate in human plasma by capillary gas chromatography with mass-selective detection.
abstract: An analytical method for the determination of norethisterone acetate (NETA) in human plasma by capillary gas chromatography-mass-selective detection (GC-MS), with testosterone acetate as internal standard, was developed and validated. After addition of the internal standard, the compounds were extracted from plasma at basic pH into diethyl ether-dichloromethane (3:2, v/v), which was then evaporated to dryness. The compounds were converted into their pentafluoropropionyl derivatives which were determined by gas chromatography using a mass selective detector at m/z 486 for NETA and m/z 476 for the internal standard. Intra-day and inter-day accuracy and precision were found suitable over the range of concentrations between 0.10 to 10 ng/ml. The method was applied to clinical samples.
authors.affiliation: Laboratoires Ciba-Geigy, Bioanalytics and Pharmacokinetics, Rueil Malmaison, France.
authors.forename: F
authors.initials: F
authors.lastname: Pommier
authors.forename: A
authors.initials: A
authors.lastname: Sioufi
authors.forename: J
authors.initials: J
authors.lastname: Godbillon
mesh_terms: D002138:Calibration; D002940:Circadian Rhythm; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D009640:Norethindrone / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D000077563:Norethindrone Acetate; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry
chemical_list: D013739:Testosterone; C040594:testosterone acetate; D000077563:Norethindrone Acetate; D009640:Norethindrone