Methods and reagents. Purification of GST fusion proteins.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds.reagnts, available on the Internet. This month's column discusses some common problems associated with the construction of protein fusions with glutathione S-transferase and with hybridization membranes. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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PN
National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
D003195:Computer Communication Networks; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics*; D008567:Membranes, Artificial; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification*
D008567:Membranes, Artificial; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D010450:Endopeptidases