A water-soluble antitumor glycoprotein from Chlorella vulgaris.
An active substance with antitumor activity (ARS2) was purified from the culture media of Chlorella vulgaris and found to be a glycoprotein with a molecular weight of 63,100 amu, as determined by matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry. ARS2 contains 66.9% carbohydrate, mainly D-galactose, and 35.2% protein. The carbohydrate moiety has a beta-1,6-D-galactopyranose backbone, as determined by methylation analysis and 13C-NMR. Apparently, the protein moiety, whose 15 amino acid sequence at the NH2-terminus, we determined as DVGEAFPTVVDALVA, is necessary for the antitumor activity, as assessed by hydrazinolysis, periodate oxidation, and proteolysis.
Noda
K
K
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Ohno
N
N
Tanaka
K
K
Kamiya
N
N
Okuda
M
M
Yadomae
T
T
Nomoto
K
K
Shoyama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000627:therapeutic use; D002708:Chlorella; D005354:Fibrosarcoma / Q000188:drug therapy*; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts; D010946:Plants, Medicinal; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D006023:Glycoproteins; D010446:Peptide Fragments; D010936:Plant Extracts; D005690:Galactose