title: In vitro blood compatibility evaluation of hollow fibre membrane using a controlled flow system: a comparative study.
abstract: A procedure has been established for the in vitro assessment of hollow fibre haemodialysis membranes. A 30 ml syringe containing 20 ml of fresh non-anticoagulated blood was mounted onto a non-pulsatile syringe pump and blood was perfused through minimodules constructed from 80 fibres retrieved from Cuprophan (Baxter ST15), cellulose acetate (M57-12, JMS Co Ltd, Hiroshima, Japan), and AN69HF (Filtral 20, Hospal, France) dialysers. Samples were collected before perfusion, 3, 6, 9 and 12 minutes. The modules were clamped vertically to minimise the effect of red cell pooling and the dialysate compartment was filled with 0.9% saline to minimise ultrafiltration. After sample processing, complement C3a, thrombin-antithrombin III complexes, prothrombin F1 + 2, and factor XII-like activity were evaluated. The results indicated that the system could discriminate between the membranes evaluated and therefore was a relevant procedure for the assessment of hollow fibre haemodialysis membranes.
authors.affiliation: Bioengineering Unit, University of Strathclyde, Glasgow, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Matata
authors.forename: H Q
authors.initials: HQ
authors.lastname: Yin
authors.forename: J M
authors.initials: JM
authors.lastname: Courtney
authors.forename: J D
authors.initials: JD
authors.lastname: Gaylor
authors.forename: N M
authors.initials: NM
authors.lastname: Lamba
authors.forename: G D
authors.initials: GD
authors.lastname: Lowe
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Izumi
authors.forename: H
authors.initials: H
authors.lastname: Klinkmann
mesh_terms: D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials / Q000592:standards*; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D001800:Blood Specimen Collection; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism; D005174:Factor XII / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D010447:Peptide Hydrolases / Q000378:metabolism; D011516:Prothrombin / Q000032:analysis; D011673:Pulsatile Flow; D006435:Renal Dialysis / Q000592:standards*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; C046193:antithrombin III-protease complex; C005062:acetylcellulose; D015926:Complement C3a; D000990:Antithrombin III; D011516:Prothrombin; D005174:Factor XII; D002482:Cellulose; C009865:cuprammonium cellulose; D010447:Peptide Hydrolases