Detection frequencies and the colony-forming unit recovery of oral treponemes by different cultivation methods.
Selected media were compared for primary isolation and detection of oral treponemes from clinical samples. Forty-eight subgingival plaque samples from 45 patients suffering from periodontitis were anaerobically cultivated for 2 weeks at 37 degrees C. Of the 9 media studied, Medium 10 (M10), which was supplemented with 10% rabbit serum and incubated using the plate-in-bottle method, supported the highest colony-forming units of the anaerobes. The treponemal colonies were detected at least on one medium from 83% of the subgingival plaque samples. The new oral spirochete medium in an anaerobic chamber supported the highest detection frequency of the oral treponemes (64% of samples); however, M10 in the plate-in-bottle was found to produce the highest colony-forming unit recovery of the oral treponemes (median 3.6% of the total colony-forming units). This study suggests that M10 in the plate-in-bottle and new oral spirochete medium in the anaerobic chamber are essential in cultivating oral treponemes.
Koseki
T
T
Dentistry of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Benno
Y
Y
Zhang-Koseki
Y J
YJ
Umeda
M
M
Ishikawa
I
I
D000693:Anaerobiosis; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology; D014208:Treponema / Q000254:growth & development* / Q000302:isolation & purification*
D003470:Culture Media