Effect of S-ethylisothiourea, a putative inhibitor of inducible nitric oxide synthase, on mouse skin vascular permeability.
By dye leakage in mouse skin, we evaluated the inhibition of proinflammatory stimuli-induced plasma extravasation by a putative inhibitor of inducible nitric oxide synthase, S-ethylisothiourea. A low dose of S-ethylisothiourea (5 micrograms/kg) mimicked aminoguanidine in inhibiting the plasma extravasation elicited by lipopolysaccharide but not by 5-hydroxytryptamine or platelet-activating factor. A higher dose of S-ethylisothiourea (10 micrograms/kg) inhibited the plasma extravasation induced by 5-hydroxytryptamine slightly; however, it increased the basal dye leakage. Thus, S-ethylisothiourea may be used as a relatively specific inhibitor for inducible nitric oxide synthase in vivo.
Muraki
T
T
Department of Pharmacology, Tokyo Women's Medical College, Japan.
Fujii
E
E
Okada
M
M
Horikawa
H
H
Irie
K
K
Ohba
K
K
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007550:Isothiuronium / Q000031:analogs & derivatives* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D010972:Platelet Activating Factor / Q000494:pharmacology; D012486:Salmonella typhimurium; D012701:Serotonin / Q000494:pharmacology; D012867:Skin / Q000098:blood supply*
D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D010972:Platelet Activating Factor; D007550:Isothiuronium; C007687:etiron; D012701:Serotonin; D019001:Nitric Oxide Synthase