title: Plasmalogen and anionic phospholipid dependence of the cardiac sarcolemmal sodium-calcium exchanger.
abstract: Although plasmalogens are the predominant phospholipids of cardiac sarcolemma, their physiological role has not been forthcoming. Since the cardiac sarcolemmal sodium-calcium exchanger has been proposed to be regulated by anionic phospholipids, the roles of plasmalogens and anionic phospholipids as regulators of the sodium-calcium exchanger were explored. Reconstituted sodium-calcium exchange activity in plasmalogen-containing proteoliposomes was 10-fold higher than that in control proteoliposomes comprised of only diacyl phospholipids. Additionally, exchange activity in plasmalogen-containing proteoliposomes was regulated by anionic phospholipids. Thus, plasmalogens provide a critical lipid environment in which anionic phospholipids serve as boundary lipids for the regulation of the trans-sarcolemmal sodium-calcium exchanger.
authors.affiliation: Department of Biochemistry and Molecular Biology, St. Louis University Health Sciences Center, MO 63104, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Ford
authors.forename: C C
authors.initials: CC
authors.lastname: Hale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000502:physiology*; D010955:Plasmalogens / Q000737:chemistry / Q000502:physiology*; D012508:Sarcolemma / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger
chemical_list: D002352:Carrier Proteins; D008081:Liposomes; D010455:Peptides; D010718:Phosphatidylserines; D010743:Phospholipids; D010955:Plasmalogens; D019831:Sodium-Calcium Exchanger; C032459:choline plasmalogens; C020791:phosphatidal ethanolamines; C066745:exchanger inhibitory peptide