Epitopes of Bordetella pertussis lipopolysaccharides as potential markers for typing of isolates with monoclonal antibodies.
Three hybridomas (P1P3, D7 and 60.5) producing monoclonal antibodies (mAbs) against Bordetella pertussis lipopolysaccharide (LPS) were established. All reacted with the LPS from a typical, vaccine strain of B. pertussis (1414), but not with that of a variant strain (A100). Two of these mAbs (P1P3 and 60.5) cross-reacted with a B. bronchiseptica LPS; only one (P1P3) reacted with a B. parapertussis LPS. ELISA reactivities with intact LPSs, and defined partial structures covalently linked to bovine serum albumin, were compared. mAb 60.5 bound to the terminal region of a distal trisaccharide consisting of N-acetylated amino sugars. D7 reacted with a substructure which can be modified in the B. parapertussis and B. bronchiseptica LPSs by addition of a polymeric O-chain. P1P3 bound to a nonacetylated glucosamine substituted with L-glycero-D-manno-heptose, present in the 'core' of the B. pertussis LPS. These mAbs may be useful for rapid typing of Bordetella in clinical isolates.
Blay
Karine Le
KL
Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
Caroff
Martine
M
Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
Blanchard
Frédéric
F
Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
Perry
Malcolm B
MB
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
Chaby
Richard
R
Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry; D015373:Bacterial Typing Techniques; D015415:Biomarkers; D001884:Bordetella / Q000276:immunology; D001886:Bordetella pertussis / Q000145:classification* / Q000276:immunology*; D002240:Carbohydrate Sequence; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006825:Hybridomas / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015415:Biomarkers; D000939:Epitopes; D008070:Lipopolysaccharides; D012710:Serum Albumin, Bovine