Pericellular pO2 as an alternative method to test cytotoxicity.
Pericellular pO2 (ppcO2) was compared with the release of cytoplasmatic enzyme lactate dehydrogenase (LDH) and mitochondrial metabolic function (tetrazolium salt reduction, MTT) as an alternative method to evaluate cytotoxicity. L-929 cells were seeded and incubated with 3 different control materials to test their cytotoxicity effects by these methods. High density polyethylene and copper were respectively used as negative and positive controls, while a 0.9% NaCl solution was as reagent control and extraction vehicle. PpcO2 was measured by a rod polarographic Clark-type probe connected to Licox pO2 computer (GMS mbH, Kiel, Germany). One-way ANOVA test showed significant differences among groups regarding every methods (p < 0.0005). The comparison of the mean variation coefficients of three methods showed no significant differences. In addition, a significant correlation was found between ppcO2 and MTT (r = 0.621; p < 0.001) as well as ppcO2 and LDH data (r = 0.474; p < 0.001). In conclusion, ppcO2 may be considered a reliable, safely and alternative method to test cytotoxicity.
Giavaresi
G
G
Servizio di Chirurgia Sperimentale, Istituto di Ricerca Codivilla-Putti, Istituto Ortopedico Rizzoli, Bologna, Italy.
Torricelli
P
P
Fini
M
M
Giardino
R
R
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis*; D011048:Polarography / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*
D013778:Tetrazolium Salts; D007770:L-Lactate Dehydrogenase; D010100:Oxygen