title: Amino acid sequence of the monomer subunit of the extracellular hemoglobin of the earthworm, Pheretima hilgendorfi.
abstract: The complete amino acid sequence of the monomer subunit of Pheretima hilgendorfi hemoglobin was determined: It consists of 140 amino acid residues, including a disulfide bond but no methionine, and has a molecular weight of 16,107 Da. Using computed analyses (amino acid maximum homology) with known sequences of monomer subunits of earthworm's hemoglobins, 115 (82%) were found to be identical with those in the corresponding positions of chain I (monomer subunit) of Pheretima sieboldi hemoglobin; 81 residues (55%), 71 residues (47%), and 66 residues (43%) were found to be in identical positions of the sequences of chain I of Lumbricus terrestris hemoglobin, chain I of Tubifex tubifex hemoglobin and chain I of Tylorrhynchus heterochaetus hemoglobin. Orthologous sequence data of monomer globins that belong to the strain A of annelid hemoglobins are discussed as useful clues for investigation of the divergence pattern of Pheretima species.
authors.affiliation: Department of Biology, Nihon University School of Medicine, Ohyaguchi-kami machi, Itabashi-ku, Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Shishikura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D006454:Hemoglobins