title: Lysophosphatidylserine enhances exogenous type II phospholipase A2-induced activation of rat serosal mast cells.
abstract: We have previously shown that exogenous type II phospholipase A2 (PLA2) alone elicits degranulation of mast cells, including rat serosal mast cells (SMC) and mouse bone marrow-derived mast cells (BMMC). Here we report that lysophosphatidylserine (lysoPS), a co-factor for activation of rodent SMC in response to some tyrosine kinase-coupled agonists, enhanced type II PLA2-elicited histamine release from rat SMC. In contrast, mouse BMMC was insensitive to lysoPS. Our findings demonstrate a novel route for activation of SMC in that type II PLA2 can act as a direct activator of SMC with enhancement by lysoPS, which is generated from membrane phosphatidylserine possibly by the action of the same enzyme.
authors.affiliation: Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: I
authors.initials: I
authors.lastname: Kudo
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D008246:Lysophospholipids / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008246:Lysophospholipids; C025059:lysophosphatidylserine; D010741:Phospholipases A; D054467:Phospholipases A2