The activity of a blood type B specific exoglycosidase from Glycine max.
Soluble antigens, enzyme-linked immunosorbent assays (ELISA), and cell suspension assays were used to study the blood group B activity of Glycine max (soybean) alpha-D-galactosidase. The enzyme readily hydrolyzed the terminal alpha-D-galactosyl of the B antigen under a variety of conditions, converting it to H antigen. Conversion of the B antigen to H antigen produces blood type O which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type B to O erythrocytes if efficient large-scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.
Hobbs
L
L
Department of Pathology, University of Missouri, Columbia 65212, USA.
Mitra
M
M
Phillips
R
R
Smith
D
D
D000017:ABO Blood-Group System / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000097:blood; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D013025:Glycine max / Q000201:enzymology*
D000017:ABO Blood-Group System; D000939:Epitopes; D006026:Glycoside Hydrolases