title: Characterisation and expression of a stage-regulated gene of Leishmania major.
abstract: This paper describes the isolation and characterisation of the meta 1 gene from Leishmania major, that is expressed predominantly in infective metacyclic parasites. The termini of the major 2-kb transcript derived from this gene have been mapped and polypyrimidine sequences implicated in RNA processing located in the flanking regions. The meta 1 gene is conserved in both Old and New World Leishmania species and codes for a putative protein of 112 amino acids. Antibodies raised against a recombinant protein expressed from this open reading frame recognise an 11.5-kDa protein in metacyclic cell lysates and this molecule localises to the region of the flagellar pocket by indirect immunofluorescence.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: F
authors.initials: F
authors.lastname: Nourbakhsh
authors.forename: S R
authors.initials: SR
authors.lastname: Uliana
authors.forename: D F
authors.initials: DF
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D017125:Genes, Protozoan; D018320:Leishmania major / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: C100528:Meta1 protein, Leishmania major; D015800:Protozoan Proteins