title: Characterization of lipoproteins during human cholestasis.
abstract: We have characterized changes in lipoproteins from cholestatic individuals and reproduced them by incubating lipoproteins from healthy individuals with cholic acid. The cholestatic patients showed an increase in low density lipoprotein (LDL) (>85%), with a smaller proportion of esterified cholesterol, and a fall in high density lipoprotein (HDL) (<10%), with a larger proportion of phospholipids. The protein composition of cholestatic HDL1 was characterized by a smaller proportion of apo A (I, II) and a prominent apo E fraction (39% vs. 9%). These changes involved an increase in degree of molecular packing (order) of HDL1. The addition of cholic acid to serum from healthy individuals altered the lipoprotein distribution, with an increase in LDL, the disappearance of HDL2 and HDL3 and the appearance of HDL1. These HDL1 were characterized by increased phospholipid and reduced apo AI fractions. They also showed a lower density and appeared as spherical particles in contrast to cholestatic HDL. Incubation of healthy HDL with cholic acid in vitro reproduces some of the alteration observed in cholestatic HDL.
authors.affiliation: Service de Biochimie A, Hopital Cochin, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Tallet
authors.forename: M P
authors.initials: MP
authors.lastname: Vasson
authors.forename: R
authors.initials: R
authors.lastname: Couderc
authors.forename: G
authors.initials: G
authors.lastname: Lefevre
authors.forename: D
authors.initials: D
authors.lastname: Raichvarg
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D001651:Cholestasis, Extrahepatic / Q000097:blood*; D002780:Cholestasis, Intrahepatic / Q000097:blood*; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D005454:Fluorescence Polarization; D006801:Humans; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000187:drug effects; D008075:Lipoproteins, HDL / Q000737:chemistry
chemical_list: D002793:Cholic Acids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D019826:Cholic Acid