title: Comparative effect of C3a and C5a on adhesion molecule expression on neutrophils and endothelial cells.
abstract: Complement activation is known to enhance neutrophil binding to human umbilical vein endothelial cells (HUVECs). Recently, we have shown that recombinant human C5a upregulates P-selectin in HUVECs. Unstimulated human neutrophil binding is also increased on C5a stimulated HUVECs. We demonstrate in this report that C5a upregulates CD11b/CD18 in human neutrophils. Also shown is that synthetic C3a57-77 and an analog 15 amino acid C3a peptide (C3a15) neither upregulate CD11b/CD18 nor do the C3a peptides increase P-selectin, ICAM-1 or E-selectin in HUVECs. Thus C5a and not C3a is responsible for early (approximately 30 minutes) neutrophil adhesion to endothelial cells after complement activation.
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Foreman
authors.forename: M M
authors.initials: MM
authors.lastname: Glovsky
authors.forename: R L
authors.initials: RL
authors.lastname: Warner
authors.forename: S J
authors.initials: SJ
authors.lastname: Horvath
authors.forename: P A
authors.initials: PA
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D018821:CD18 Antigens / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015926:Complement C3a / Q000494:pharmacology*; D015936:Complement C5a / Q000494:pharmacology* / Q000502:physiology; D019040:E-Selectin / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D016177:Macrophage-1 Antigen / Q000502:physiology; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D019007:P-Selectin / Q000096:biosynthesis* / Q000235:genetics; D010446:Peptide Fragments / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014471:Umbilical Veins
chemical_list: D018821:CD18 Antigens; D019040:E-Selectin; D016177:Macrophage-1 Antigen; D019007:P-Selectin; D010446:Peptide Fragments; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D015926:Complement C3a; D015936:Complement C5a