3' Noncoding sequences of the CTA 1 gene enhance expression of the recombinant serine protease inhibitor, CPTI II, in Saccharomyces cerevisiae.
Expression of the gene coding for the recombinant trypsin inhibitor, CPTI II, was enhanced tenfold when yeast transcription terminating sequences were added to the expression cassette of the pJK6 yeast vector. The yield was further increased about 20% in the BJ5464 yeast strain, defective in vacuolar proteases.
Stankiewicz
M
M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Rempoła
B
B
Fikus
M
M
D001483:Base Sequence; D002374:Catalase / Q000235:genetics*; D004274:DNA, Recombinant; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014361:Trypsin Inhibitors / Q000235:genetics*
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D014361:Trypsin Inhibitors; D002374:Catalase