Actin mRNA is not lowered in chronic myeloid leukemic granulocytes.
Chronic myeloid leukemic (CML) granulocytes exhibit defects in several functions, some of which have been associated with changes in the expression of cell surface molecules, actin reorganization and lowered levels of total cellular actin. In this study, we show by northern blotting that the steady-state level of mRNA for actin is not decreased in the CML granulocyte. Our data suggest that the lowered levels of actin protein in the leukemic granulocyte may be due to altered control at the translational/post-translational step, rather than at the level of transcription/post-transcription, implicated in the regulation of expression of the surface molecules, Fc gamma RII, Fc gamma RIII and alkaline phosphatase.
Kant
A M
AM
Cancer Research Institute, Parel, Bombay, India.
Advani
S H
SH
Zingde
S M
SM
D000199:Actins / Q000032:analysis*; D015152:Blotting, Northern; D006098:Granulocytes / Q000737:chemistry*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D012333:RNA, Messenger / Q000032:analysis*; D012337:RNA, Ribosomal, 18S / Q000032:analysis
D000199:Actins; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S