Combined effects of constant sublethal UVA irradiation and elevated temperature on the survival and general metabolism of the convict-cichlid fish, Cichlasoma nigrofasciatum.
In a previous study we observed that a constant sublethal UVA (320-400 nm) irradiation had a significant effect on the general metabolism in the Convict-cichlid fish (Cichlasoma nigrofasciatum) [Winckler, K. and Fidhiany, L. (1996) J. Photochem. Photobiol. B. Biol. (In press)]. In the present study we show that sublethal UVA irradiation in combination with elevated environmental temperature has a deleterious effect on the same population. The threshold temperature for a sudden increase in mortality of fish receiving an additional sublethal UVA irradiation was 32 degrees C. Prior to the increased mortality, the fish started to avoid the UV light source when the water temperature increased to 31.5 degrees C. Mortality decreased when the temperature declined below 31.5 degrees C. As soon as the temperature changed to normal (adapted) condition (27-29 degrees C) mortality returned to normal levels. In contrast, no changes of fish behavior or mortality were observed at elevated temperature in the nonirradiated reference population. The percentages of fish surviving the high temperature stress were 21.9% for the UVA population and 96.8% for the reference population. The specific oxygen consumption (SOC, average +/- SD) of the survivors from the UVA population during temperature stress was 0.21 +/- 0.05 mg O2 h-1 g body weight (BW)-1, while it was 0.54 +/- 0.11 mg O2 h-1 g BW-1 in the reference population. After the environmental temperature returned below the apparent upper temperature tolerance limit, the oxygen consumption of the UVA population gradually normalized. The SOC measured at different temperature levels--after after the fish passed the temperature stress--showed no significant differences between the UVA population and its reference at 23, 25, 27 and 29 degrees C. However, the SOC at 31 degrees C was significantly (P < 0.05) lower than reference, while at 33 degrees C it was higher (P < 0.10).
Winckler
K
K
Institut für Biophysik, Freie Universität Berlin, Germany. xbiokw@fub46.zedat.fu-berlin.de
Fidhiany
L
L
D000222:Adaptation, Physiological / Q000528:radiation effects; D000818:Animals; D001522:Behavior, Animal / Q000528:radiation effects; D006358:Hot Temperature / Q000009:adverse effects; D010101:Oxygen Consumption / Q000528:radiation effects; D010471:Perches / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism; D013696:Temperature; D014329:Tropical Climate; D014466:Ultraviolet Rays / Q000009:adverse effects*