Biological activities of lipopolysaccharide of Burkholderia (Pseudomonas) pseudomallei.
Endotoxic activities of lipopolysaccharide (LPS) isolated from Burkholderia (Pseudomonas) pseudomallei, a causative agent of melioidosis, were investigated. Compared to an enterobacterial LPS (SAE-LPS), B. pseudomallei LPS (BP-LPS) exhibited weaker pyrogenic activity in rabbits, lethal toxicity in galactosamine-sensitized mice and murine macrophage activation, i.e. production of tumor necrosis factor, interleukin-6 and nitric oxide. BP-LPS, on the other hand, exhibited stronger mitogenic activity to murine splenocytes than SAE-LPS; moreover, it stimulated even the splenocytes of LPS-resistant C3H/HeJ mice. Unusual chemical structures in the acid-stable inner core region attached to the lipid A moiety of BP-LPS may be responsible for this strong mitogenic activity.
Matsuura
M
M
Department of Microbiology, Jichi Medical School, Tochigi, Japan.
Kawahara
K
K
Ezaki
T
T
Nakano
M
M
D000818:Animals; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D066298:In Vitro Techniques; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity*; D008213:Lymphocyte Activation / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D008554:Melioidosis / Q000209:etiology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000633:toxicity; D011749:Pyrogens / Q000633:toxicity; D011817:Rabbits; D012475:Salmonella / Q000472:pathogenicity; D013045:Species Specificity; D014774:Virulence
D008070:Lipopolysaccharides; D008934:Mitogens; D011749:Pyrogens