A Brevibacterium linens pRBL1 replicon functional in Corynebacterium glutamicum.
Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pXZ10142 (C. glutamicum) and pAL5000 (Mycobacterium fortuitum). The 310-aa protein also shows significant homology to proteins of pColE5-099 (Shigella sonnei) and pJD1 (Neisseria gonorrhoea). At least one of these proteins, the Rep protein of pColE5-099, is known to be involved in theta replication.
Ankri
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S
Institut de Génétique et Microbiologie URA, Université de Paris-Sud, Orsay, France.
Bouvier
I
I
Reyes
O
O
Predali
F
F
Leblon
G
G
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001951:Brevibacterium / Q000235:genetics*; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D005822:Genetic Vectors / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000502:physiology*; D012093:Replicon / Q000502:physiology*
D001426:Bacterial Proteins