title: Opposite developmental regulation of P- and Q-type calcium currents during ontogenesis of large diameter mouse sensory neurons.
abstract: Analysis of neuronal development has emphasized the importance of voltage-activated Ca2+ currents during the initial period of differentiation. We investigated non-N, non-L Ba2+ currents through Ca2+ channels in freshly dissociated large diameter embryonic mouse dorsal root ganglion neurons using the whole-cell patch-clamp technique. Two types of omega-agatoxin IVA-sensitive currents were clearly distinguished at embryonic day 13: a sustained P-type current blocked selectively at 30 nM (IC50 = 3nM) and an inactivating Q-type current blocked in the range 50-500 nM (IC50 = 120nM). The P-type Ca2+ current disappeared at day 15 whereas the Q-type Ca2+ current increased two- to three-fold during the same embryonic period. In contrast, the contribution of the non-L, non-N, omega-agatoxin IVA-resistant current (R-type) was constant during this developmental span. In conclusion, our results clearly show that P- and Q-type Ca2+ currents are differentially expressed during ontogenesis in large diameter dorsal root ganglion neurons. The developmental change, which occurs during the period of target innervation, could be related to specific key events such as natural neuron death and onset of synapse formation.
authors.affiliation: Laboratoire de médecine expérimentale, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Hilaire
authors.forename: S
authors.initials: S
authors.lastname: Diochot
authors.forename: G
authors.initials: G
authors.lastname: Desmadryl
authors.forename: M
authors.initials: M
authors.lastname: Baldy-Moulinier
authors.forename: S
authors.initials: S
authors.lastname: Richard
authors.forename: J
authors.initials: J
authors.lastname: Valmier
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000096:biosynthesis*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005727:Ganglia, Spinal / Q000196:embryology / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009475:Neurons, Afferent / Q000166:cytology / Q000502:physiology*; D009568:Nitrendipine / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D013111:Spider Venoms / Q000494:pharmacology; D013997:Time Factors; D020867:omega-Agatoxin IVA
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D013111:Spider Venoms; D020867:omega-Agatoxin IVA; D001464:Barium; D009568:Nitrendipine