Movement of the Na+ channel inactivation gate during inactivation.
Phenylalanine 1489 in the inactivation gate of the rat brain IIA sodium channel alpha subunit is required for stable inactivation. It is proposed to move into the intracellular mouth of the pore and occlude it during inactivation, but direct evidence for movement of this residue during inactivation has not been presented. We used the substituted cysteine accessibility method to test the availability of a cysteine residue substituted at position 1489 to modification by methanethiosulfonate reagents applied from the cytoplasmic side. Mutation of Phe-1489 to Cys results in a small (8%) fraction of noninactivating current. Ag+ and methanethiosulfonate reagents irreversibly slowed the inactivation rate and increased the fraction of noninactivating current of F1489C but not wild-type channels. Single channel analysis showed that modification slowed inactivation from both closed and open states and destabilized the inactivated state. Depolarization prevented rapid modification of Cys-1489 by these reagents, and the voltage dependence of their reaction rate correlated closely with steady-state inactivation. Modification was not detectably voltage-dependent at voltages more negative than channel gating. Our results show that, upon inactivation, Phe-1489 in the inactivation gate moves from an exposed and modifiable position outside the membrane electric field to a buried and inaccessible position, perhaps in or near the intracellular mouth of the channel pore.
Kellenberger
S
S
Department of Pharmacology, University of Washington, Seattle, Washington 98195-7280, USA.
Scheuer
T
T
Catterall
W A
WA
D000818:Animals; D001921:Brain / Q000502:physiology; D003545:Cysteine / Q000737:chemistry; D015640:Ion Channel Gating; D009038:Motion; D009068:Movement; D016297:Mutagenesis, Site-Directed; D009865:Oocytes; D051381:Rats; D011994:Recombinant Proteins; D015222:Sodium Channels / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000737:chemistry; D014982:Xenopus laevis
D011994:Recombinant Proteins; D015222:Sodium Channels; D013439:Sulfhydryl Reagents; D003545:Cysteine