A cDNA cloning of human AEBP1 from primary cultured osteoblasts and its expression in a differentiating osteoblastic cell line.
By comparing lists of 3'-directed partial cDNA sequences (gene signatures) randomly collected from various tissues, genes uniquely expressed in individual tissue can be identified. A full length cDNA clone, corresponding to one such gene signature, unique to human osteoblast and adipose tissue, was isolated. This cDNA clone encodes a 845-amino acid protein that is almost identical to the mouse adipocyte transcription factor AEBP1 except that it has additional 105 amino acids in the N terminus. A northern hybridization showed that in the differentiating murine osteoblastic cell line, AEBP1 is also expressed but that it is shut off in the final calcification phase, suggesting a transcriptional repressive effect on genes for bone formation.
Ohno
I
I
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Hashimoto
J
J
Shimizu
K
K
Takaoka
K
K
Ochi
T
T
Matsubara
K
K
Okubo
K
K
D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002268:Carboxypeptidases; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
C484818:AEBP1 protein, human; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D018076:DNA, Complementary; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; D002268:Carboxypeptidases