Novel aspects of pheromone-induced cell-cycle arrest in yeast.
Saccharomyces cerevisiae responds to pheromones by arresting cell-cycle progression at the G1/S boundary. This arrest has been shown to require the activation by phosphorylation of Far1, a cyclin-dependent kinase inhibitor. Arrest resembling pheromone-induced arrest can be triggered by high levels of Ste12, the pheromone-responsive transcriptional activator. In the present study, cell-cycle arrest induced by high levels of Ste12 is characterized. This arrest occurs predominately at the G1/S boundary and is independent of Far1 and upstream components of the pheromone response pathway. Since Ste12 is a transcriptional activator, it is proposed that cell-cycle arrest in response to high levels of Ste12 is due to transcription of a Ste12-dependent arrest factor regulated primarily, but not necessarily exclusively, at the level of transcription. The data presented here suggest that a novel component has a role in arrest in response to pheromone.
Dolan
J W
JW
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA.
D001685:Biological Factors / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D004171:Diploidy; D005656:Fungal Proteins / Q000378:metabolism; D007700:Kinetics; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D010675:Pheromones / Q000494:pharmacology; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D001685:Biological Factors; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C081353:FAR1 protein, S cerevisiae; D005656:Fungal Proteins; D010455:Peptides; D010675:Pheromones; D012097:Repressor Proteins; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000072235:Mating Factor