In vitro chemotherapy of steroid receptor positive human meningioma low-passage primary cultures with nitrosourea-methionine-steroid conjugates.
The antiproliferative activity of two nitrosourea-methionine-steroid conjugates, N-[N'-(2-chloroethyl)-N'-nitrosocarbamoyl] (CNC)-L-methionine-testosterone-17-ester and CNC-L-methionine-dihydrotestosterone-17-ester, was investigated in two meningioma low-passage primary cultures that had been derived from human meningiomas and characterized with regard to proliferation pattern and progesterone receptor content. Treatment was given over 4 days. Cytotoxicity (concentrations investigated: 50, 100, and 200 microM) was compared with the effects of the equimolar mixtures of CNC-Met plus steroid. The steroid-linked nitrosoureas were superior to their unlinked components. CNC-Met itself displayed no or marginal activity, whereas the steroid component displayed significant activity.
Taut
F J
FJ
German Cancer Research Center, Heidelberg, Germany.
Zeller
W J
WJ
D000970:Antineoplastic Agents / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D008577:Meningeal Neoplasms / Q000188:drug therapy* / Q000648:ultrastructure*; D008579:Meningioma / Q000188:drug therapy* / Q000648:ultrastructure*; D008875:Middle Aged; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D011987:Receptors, Steroid / Q000032:analysis*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C104207:N-(N'-(2-chloroethyl)--N'-nitrosocarbamoyl)methionine-dihydrotestosterone-17-ester; C104206:N-(N'-(2-chloroethyl)-N'--nitrosocarbamoyl)methionine-testosterone-17-ester; D009607:Nitrosourea Compounds; D011987:Receptors, Steroid; D013739:Testosterone