Promoter analysis of a human dihydrodiol dehydrogenase.
The 5' flanking region of a human dihydrodiol dehydrogenase (DDH) gene was isolated and sequenced from bp +124 to -1161. It contains putative binding sites for liver specific factors including NF-IL6 and HNF-5 sites and AP-1, AP-2 and NF kappa B-like sites. Sequence analysis identified this gene as a type II DDH. Reporter analysis of deletion constructs transfected in HepG2 cells identified negative regulatory regions and a minimal promoter (-104 to +65) containing two AP-2 sites in tandem.
Ciaccio
P J
PJ
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA. RAHPJC@RohmHaas.com
Walsh
E S
ES
Tew
K D
KD
D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D018808:Transcription Factor AP-1 / Q000378:metabolism; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D009687:Nuclear Proteins; D018808:Transcription Factor AP-1; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D004247:DNA; D010088:Oxidoreductases; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase