Molecular cloning of a partial cDNA of a novel gene in iron metabolism.
In order to elucidate the possible role played by human hepatic ferritin-associated protein in normal cellular iron metabolism and in iron overload diseases, malignancies and hepatic fibrosis, we attempted to isolate genes for ferritin associated proteins. Here we describe the cloning of a novel cDNA fragment of a 10 kb mRNA transcript from human liver and human T lymphoid (MOLT-4) cells using a short degenerate 5'-primer derived from six amino acid residues of an affinity purified ferritin associated protein from human liver. Northern analysis of this clone has revealed the presence of a 10 kb mRNA species in both human liver and MOLT-4 cell RNA.
Kedar
V
V
Liver-Unit, Queensland Institute of Medical Research, Brisbane, Australia.
Moss
D
D
Halliday
J
J
Powell
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002268:Carboxypeptidases; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D007501:Iron; D002268:Carboxypeptidases; C102497:ferritin associated protein, human