A microbiological assay for the quantitative determination of glutathione.
Based on the requirement of a glutathione-deficient mutant strain of Saccharomyces cerevisiae to take up external glutathione for growth on synthetic media, a simple agar diffusion test for quantitative detection of total glutathione from various sources was established. Glutathione concentrations can be reliably detected in a less expensive way, requiring less technical effort compared to enzymatic or biochemical detection methods.
Schmidt
M
M
Johann Wolfgang Goethe-Universität, Frankfurt, Germany.
Grey
M
M
Brendel
M
M
D005978:Glutathione / Q000032:analysis*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D005978:Glutathione