Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae.
The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains.
Sperandio
V
V
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA.
Bailey
C
C
Girón
J A
JA
DiRita
V J
VJ
Silveira
W D
WD
Vettore
A L
AL
Kaper
J B
JB
D018829:Adhesins, Bacterial / Q000235:genetics*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D014734:Vibrio cholerae / Q000235:genetics*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; C096349:OmpU protein, Vibrio cholerae