Characterization of cytotoxic, hemolytic Aeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene.
Aeromonas caviae has recently been recognized as an important enteropathogen and its hemolysin is purported to be one of the virulence factors, In this study, a total of 80 clinical isolates of Aeromonas spp. were investigated by PCR with synthetic oligonucleotides targeting a cloned hemolysin-encoding sequence from an A. caviae isolate of clinical origin. Of the 35 clinical A. caviae isolates tested, only 6 contained the target sequence.
Wang
G
G
Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, Canada. gwang@hpb.hwc.ca
Tyler
K D
KD
Munro
C K
CK
Johnson
W M
WM
D000333:Aeromonas / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D006460:Hemolysin Proteins