title: The activity of a blood type B specific exoglycosidase from Glycine max.
abstract: Soluble antigens, enzyme-linked immunosorbent assays (ELISA), and cell suspension assays were used to study the blood group B activity of Glycine max (soybean) alpha-D-galactosidase. The enzyme readily hydrolyzed the terminal alpha-D-galactosyl of the B antigen under a variety of conditions, converting it to H antigen. Conversion of the B antigen to H antigen produces blood type O which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type B to O erythrocytes if efficient large-scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.
authors.affiliation: Department of Pathology, University of Missouri, Columbia 65212, USA.
authors.forename: L
authors.initials: L
authors.lastname: Hobbs
authors.forename: M
authors.initials: M
authors.lastname: Mitra
authors.forename: R
authors.initials: R
authors.lastname: Phillips
authors.forename: D
authors.initials: D
authors.lastname: Smith
mesh_terms: D000017:ABO Blood-Group System / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000097:blood; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D013025:Glycine max / Q000201:enzymology*
chemical_list: D000017:ABO Blood-Group System; D000939:Epitopes; D006026:Glycoside Hydrolases