title: Induction of the Oct-2 transcription factor in primary sensory neurons during inflammation is nerve growth factor-dependent.
abstract: The mRNA encoding the POU family transcription factor Oct-2 is induced in cultured adult sensory neurons following treatment with nerve growth factor (NGF) but not with a variety of other growth factors. We show here that the Oct-2 mRNA is also upregulated in vivo in sensory neurons innervating inflamed tissue following intraplantar injection of complete Freund's adjuvant. This rise is abolished by systemic administration of anti-NGF neutralizing antibodies indicating that it is an NGF-dependent effect. Hence a very specific aspect of the NGF response occurs in sensory neurons innervating inflamed tissue in vivo. In turn, the induction of Oct-2 may play a role in the other changes observed in such neurons both in gene expression and in their ability to respond to painful stimuli.
authors.affiliation: Department of Molecular Pathology, UCL Medical School, London, UK.
authors.forename: E
authors.initials: E
authors.lastname: Ensor
authors.forename: G
authors.initials: G
authors.lastname: Kendall
authors.forename: A
authors.initials: A
authors.lastname: Allchorne
authors.forename: C J
authors.initials: CJ
authors.lastname: Woolf
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005620:Freund's Adjuvant; D017403:In Situ Hybridization; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors / Q000502:physiology*; D009475:Neurons, Afferent / Q000378:metabolism*; D050813:Octamer Transcription Factor-2; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D013462:Sulfur Radioisotopes; D014157:Transcription Factors / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D009414:Nerve Growth Factors; D050813:Octamer Transcription Factor-2; C489205:Pou2f2 protein, rat; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014157:Transcription Factors; D005620:Freund's Adjuvant