title: Structural organization of the rae28 gene, a putative murine homologue of the Drosophila polyhomeotic gene.
abstract: A putative murine homologue of the Drosophila polyhomeotic gene, named rae28, has been isolated from a genomic library of 129/SV mouse and its structural organization has been analyzed. rae28 is a single gene of approximately 22 kb long and consists of 15 exons. Its 5'-flanking region lacks typical transcriptional regulatory sequences, such as TATA and CCAAT boxes, but contains GC-rich sequences and seven putative binding sites for a transcription factor, Sp1. One major transcription start point has been determined. The overall exon-intron organization suggested that three different Rae28 mRNAs are generated through alternative splicing. Furthermore, the rae28 gene has been located on the R-positive F3 band of mouse chromosome 6 by the direct R-banding fluorescence in situ hybridization methods.
authors.affiliation: Department of Medical Genetics, Osaka University.
authors.forename: M
authors.initials: M
authors.lastname: Abdul Motaleb
authors.forename: Y
authors.initials: Y
authors.lastname: Takihara
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Higashinakagawa
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005091:Exons; D017344:Genes, Insect; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D063150:Polycomb Repressive Complex 1; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009698:Nucleoproteins; C072820:ph-d protein, Drosophila; D063150:Polycomb Repressive Complex 1