Regulation of intracellular calcium is closely linked to glucose metabolism in J774 macrophages.
The effects of 2-deoxy-D-glucose (2dGlc) and glucose deprivation were investigated in the J774 murine macrophage-like cell line. 2dGlc addition or glucose deprivation for 4 min led to an inhibition in the transient increase in cytoplasmic free Ca2+ ([Ca2+]i) that otherwise occurs in response to three different agonists: IgG, ATP and platelet activating factor. This inhibition was preceded by a partial release of Ca2+ from intracellular, thapsigargin-sensitive stores. In contrast, the transition from 5 to 30 mM glucose caused a decrease in [Ca2+]i and a corresponding increase in thapsigargin-sensitive sequestered Ca2+. The effects of an alternate glycolytic inhibitor, NaF, and a mitochondrial inhibitor, rotenone, were also tested. These inhibitors caused neither a release of Ca2+ from intracellular stores nor an inhibition in any of the agonist responses. The capacitative influx of extracellular Ca2+ following depletion of intracellular stores was also found to be selectively inhibited by the prior addition of 2dGlc or with glucose deprivation. In addition, when an elevated plateau of [Ca2+]i was established by the irreversible depletion of intracellular Ca2+ stores, the addition of 2dGlc caused a decrease in the on-going capacitative entry of Ca2+.
Darbha
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Department of Cell Biology, University of Alabama at Birmingham 35294-0005, USA.
Marchase
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D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D002118:Calcium / Q000819:agonists / Q000378:metabolism*; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004553:Electric Conductivity; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes; D005460:Fluorides, Topical / Q000494:pharmacology; D016257:Fura-2; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007074:Immunoglobulin G / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
D000963:Antimetabolites; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D005460:Fluorides, Topical; D007074:Immunoglobulin G; D010972:Platelet Activating Factor; D014475:Uncoupling Agents; D012402:Rotenone; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D003847:Deoxyglucose; D005947:Glucose; D002118:Calcium; D016257:Fura-2