Acetylcholine releases of mesopontine PGO-on cells in the lateral geniculate nucleus in sleep-waking cycle and serotonergic regulation.
1. Five adult cats were prepared with standard sleep-recording electrodes, microinjection cannulae in the bilateral mesopontine tegmentum X area and cannulae for microdialysis probes in the LGN. 2. Dialysates were collected at 5 min intervals during SWS, REM sleep and wakefulness, and during pre- and post-stimulation periods of 5MeODMT microinjection in the bilateral mesopontine tegmentum of freely moving cats. 3. A REM sleep-specific increase of ACh release was observed in the LGN, but not out of the LGN. 4. ACh release was depressed up to 33% by bilateral 5MeODMT microinjection in the mesopontine tegmentum where the microinjection of carbachol also depressed ACh in the LGN probably by stimulating the auto receptor. 5. Our observations indicate that PGO-on neurons of mesopontine tegmentum release more ACh in the LGN during REM sleep by burst discharge than during wakefulness and SWS, and that presumptive cholinergic PGO-on cells are regulated by serotonergic inputs.
Kodama
T
T
Department of Psychology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Honda
Y
Y
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D018679:Cholinergic Agonists / Q000494:pharmacology; D004567:Electrodes, Implanted; D005260:Female; D005829:Geniculate Bodies / Q000166:cytology / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D008845:Microinjections; D009474:Neurons / Q000378:metabolism; D009778:Occipital Lobe / Q000166:cytology / Q000378:metabolism*; D011149:Pons / Q000166:cytology / Q000378:metabolism*; D012701:Serotonin / Q000502:physiology*; D012890:Sleep / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
D018679:Cholinergic Agonists; D012701:Serotonin; D002217:Carbachol; D000109:Acetylcholine