An efficient DDAB-mediated transfection of Drosophila S2 cells.
I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.
Han
K
K
Department of Genetic Engineering, Hallym University, Chunchon, Republic of Korea. khhan@sun.hallym.ac.kr
D000818:Animals; D002460:Cell Line; D004330:Drosophila; D017930:Genes, Reporter; D007763:Lac Operon; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D014162:Transfection
D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium