Effect of L-arginine on myoglobin-induced acute renal failure in the rabbit.
Because myoglobin is a potent inhibitor of nitric oxide (NO), we tested whether myoglobin infusion results in renal vasoconstriction and dysfunction, on which L-arginine, a source of NO, has a protective effect in sedated, nondehydrated, and nonacidotic rabbits. The infusion of myoglobin (375 mg/kg) resulted in a decrease in renal blood flow, an increase in renal vascular resistance, and a decrease in creatine clearance associated with a decrease in urinary excretory rate of nitrite/nitrate and guanosine 3',5'-cyclic monophosphate (cGMP). These values 1-2 h after the infusion were significantly different from baseline levels. Co-administration of L-arginine (150 mg/kg bolus followed by 150 mg.kg(-1).min(-1) reversed these changes significantly with attenuation of urinary excretory rate of nitrite/nitrate and cGMP. This study suggests that the myoglobin-induced renal vasoconstriction and dysfunction and protective effect of L-arginine on these outcomes could be mediated through the NO system.
Wakabayashi
Y
Y
Department of Medicine, University of Kitasato Medical School, Sagamihara, Japan.
Kikawada
R
R
D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D003404:Creatinine / Q000097:blood / Q000652:urine; D006152:Cyclic GMP / Q000652:urine; D008297:Male; D009211:Myoglobin; D009566:Nitrates / Q000652:urine; D009573:Nitrites / Q000652:urine; D011817:Rabbits; D012079:Renal Circulation / Q000187:drug effects; D014655:Vascular Resistance / Q000187:drug effects
D009211:Myoglobin; D009566:Nitrates; D009573:Nitrites; D001120:Arginine; D003404:Creatinine; D006152:Cyclic GMP