title: Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35nck5a in the rat retina.
abstract: Neuronal Cdc2-like kinase has been purified from the bovine brain as a proline-directed serine/threonine kinase. This kinase is a heterodimer of Cdk5 and p35nck5a and influences neuronal maturation or sprouting in the normal brain. In this study, we showed the expression of Cdk5/p35nck5a kinase in the developing rat retina. The expression of Cdk5 and p35nck5a increased between 1 week and 3 weeks after birth. These expression levels were most prominent from 2 weeks to 3 weeks after birth and decreased after 4 weeks. The developmental change of Cdk5/p35nck5a kinase activity coincided with those of the expression of p35nck5a and Cdk5. An immunohistochemical study showed that Cdk5 was expressed in the ganglion cells and in some cells in the inner nuclear layer at an early stage. With retinal development, Cdk5 was expressed in the inner plexiform layer also. In the adult, the expression of Cdk5 was restricted to the inner plexiform layer and to some cells in the inner nuclear layer. These changes of localization in the developing retina were very close to those of B-50/GAP-43. On the other hand, the expression of p35nck5a was restricted to the soma of the neuron in the developing retina. This subcellular localization in the developing retina agreed with that in the developing rat brain. The expression levels of Cdk5 and p35nck5a in retina of rats raised from fetus to 3 weeks after birth in darkness were 36% and 40% respectively, of the baseline for control rats. Moreover, the kinase activity in rats raised in darkness was lower than that in control rats. These data suggest that Cdk5/p35nck5a may play a role in neuronal plasticity in the developing rat retina.
authors.affiliation: Department of Physiology, Faculty of Medicine, Kagawa Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirooka
authors.forename: K
authors.initials: K
authors.lastname: Tomizawa
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
authors.forename: T
authors.initials: T
authors.lastname: Itano
authors.forename: E
authors.initials: E
authors.lastname: Hasegawa
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.forename: O
authors.initials: O
authors.lastname: Hatase
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D003624:Darkness; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D010770:Phosphotransferases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000166:cytology* / Q000201:enzymology* / Q000254:growth & development; D013569:Synapses / Q000201:enzymology
chemical_list: D010770:Phosphotransferases; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495907:Cdk5 protein, rat; D018844:Cyclin-Dependent Kinases