Separation of enzymes from polyenzyme mixtures used in medicine and pharmacy. I. Isolation and characterization of xylanase from Aspergillus oryzae.
Three multiple forms (F-I, F-II and F-III) of xylanase produced from Aspergillus oryzae were established in the commercial preparation Luizym. Two methods were used (ion-exchange chromatography and polyacrylamide gel electrophoresis). The active xylanase fraction (F-III-II-I) was purified and characterized (pHopt 4.5; Topt 37 degrees C and molecular mass 38.5 kDa). The products of the enzymatic reaction were analysed by HPLC. The hydrolysis pattern showed that the xylanase was an exo-endo acting enzyme.
Bakalova
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N
Department of Biochemistry, Faculty of Biology, University of Sofia, Bulgaria.
Petrova
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S
Kolev
D
D
D001236:Aspergillus oryzae / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry / Q000302:isolation & purification*
D005656:Fungal Proteins; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase