title: Identification of a 60 kd cross-reactive allergen in pollen and plant-derived food.
abstract: BACKGROUND
  Cross-reactive IgE antibodies were found to be responsible for allergic reactions in patients allergic to pollen on ingestion of food (oral allergy syndrome). So far, the major birch pollen allergen Bet v 1 and birch profilin (Bet v 2) were identified as relevant cross-reactive allergens.
  
  
  OBJECTIVE
  In this study we attempted to identify additional cross-reactive plant allergens, which could be responsible for food intolerance in patients allergic to pollen.
  
  
  METHODS
  Monoclonal antibodies specific for the major mugwort pollen allergen, Art v 1, representing a 60 kd glycoprotein, were used to detect cross-reactive allergens in other pollens and plant-derived food. The amino acid compositions of the cross-reactive structures were determined, and their resistance against trypsin treatment was investigated. In addition, IgE immunoblot inhibitions were done with the 60 kd mugwort pollen allergen.
  
  
  RESULTS
  Monoclonal antibodies specific for the major mugwort pollen allergen, Art v 1, cross-reacted with proteins of comparable molecular weight in fruit and vegetables. Preadsorption of patients' sera with the 60 kd mugwort allergen led to a reduction of IgE binding to components of a similar molecular weight present in different pollen (birch, timothy grass), fruit (apple, peanuts), and vegetable (celery) extracts and reduced IgE binding to apple, kiwi, and celery as determined by RAST inhibitions.
  
  
  CONCLUSIONS
  A cross-reactive plant panallergen, possibly identical to the major mugwort pollen allergen, Art v 1, is described. The allergen represents a protein of approximately 60 kd present in various pollen and plant foods; which is distinct from Bet v 1 and profilin and hence may represent a novel cross-reactive allergen in the oral allergy syndrome.
authors.affiliation: Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Heiss
authors.forename: S
authors.initials: S
authors.lastname: Fischer
authors.forename: W D
authors.initials: WD
authors.lastname: Müller
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: Hirschwehr
authors.forename: S
authors.initials: S
authors.lastname: Spitzauer
authors.forename: D
authors.initials: D
authors.lastname: Kraft
authors.forename: R
authors.initials: R
authors.lastname: Valenta
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant; D003285:Contractile Proteins; D003429:Cross Reactions; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D005638:Fruit / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000276:immunology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D010945:Plants, Edible / Q000737:chemistry / Q000276:immunology*; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D051304:Profilins; D012882:Skin Tests; D014675:Vegetables / Q000276:immunology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C102724:Art v 1 protein, mugwort; D003285:Contractile Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D051304:Profilins; D007073:Immunoglobulin E