A novel heme protein that acts as a carbon monoxide-dependent transcriptional activator in Rhodospirillum rubrum.
The gene coding for a carbon monoxide-dependent transcriptional activator (cooA) in Rhodospirillum rubrum has been expressed in E. coli, and the recombinant CooA has been purified. CooA contains b-type heme which may act as a CO sensor in vivo. CO-bound CooA was formed when reduced CooA was reacted with CO, but not in the case of oxidized CooA. CooA is the first example of the heme protein acting as a DNA-binding transcriptional activator.
Aono
S
S
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan. aono@jaist.ac.jp
Nakajima
H
H
Saito
K
K
Okada
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques; D002248:Carbon Monoxide / Q000378:metabolism*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D006420:Hemeproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D013057:Spectrum Analysis; D015534:Trans-Activators / Q000378:metabolism*
D001426:Bacterial Proteins; C088904:CooA protein, E coli; D029968:Escherichia coli Proteins; D006420:Hemeproteins; D015534:Trans-Activators; D036781:Fimbriae Proteins; D002248:Carbon Monoxide