Capillary gas chromatographic method for the determination of n-butyl-p-aminobenzoate and lidocaine in plasma samples.
A fast capillary gas chromatographic method with nitrogen-selective detection is described that allows selective and reproducible quantification of n-butyl-p-aminobenzoate (BAB) and lidocaine in plasma. The sampling and sample storage conditions are critical for the quantification of BAB. Diisopropyl fluorophosphate, an organo-phosphorus pesticide, has to be added during sampling to prevent the rapid decomposition of BAB by cholinesterases.
Grouls
R J
RJ
Department of Pharmaceutical Services, Catharina Hospital, Eindhoven, Netherlands.
Machielsen
E
E
Korsten
H H
HH
Hellebrekers
L J
LJ
Breimer
D D
DD
Ackerman
E W
EW
D000779:Anesthetics, Local / Q000008:administration & dosage / Q000097:blood*; D000818:Animals; D001566:Benzocaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D001800:Blood Specimen Collection; D002138:Calibration; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002849:Chromatography, Gas; D004285:Dogs; D004355:Drug Stability; D006801:Humans; D007268:Injections, Epidural; D007531:Isoflurophate / Q000494:pharmacology; D008012:Lidocaine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000779:Anesthetics, Local; D002800:Cholinesterase Inhibitors; D007531:Isoflurophate; D008012:Lidocaine; C004605:butamben; D001566:Benzocaine