title: Nitric oxide prevents human platelet adhesion to fiber membranes in whole blood.
abstract: During cardiopulmonary bypass or long-term extracorporeal life support, foreign surface induced platelet deposition in the oxygenator causes deterioration of gas exchange. In this study, the authors evaluated the effectiveness of nitric oxide (NO) in reducing the adhesion of platelets in whole blood to the surface of hollow fiber membranes. For this purpose, a test chamber was designed consisting of a gas exchanger with ten mitsubishi multi-layered composite hollow fibers (MHF: 257 mm OD; 203 mm ID; 70 mm length) and a polypropylene tube (16 mm OD; 100 mm length). Pure N2 (control) or nitric oxide (NO) (100 ppm, 200 ppm in N2) were delivered into the test chamber previously filled with 13 ml human whole blood. Platelet counts and platelet factor 4 (PF4), as a measure of platelet activation, were measured before and after either 1 or 2 hr of testing, and fibers were observed under scanning electron microscopic study (SEM) after each experiment. In the control and 100 ppm NO groups, platelet counts decreased and the level of PF4 increased during the 1 hr period. In the 200 ppm NO group, almost no platelet deposition could be observed on the surface of fibers under SEM. In conclusion, NO flow through hollow fiber membranes can markedly reduce platelet adhesion. Additional quantitative studies should define the optimal concentration for this effect and determine if this finding could improve oxygenator function, especially under conditions of long-term support.
authors.affiliation: Jichi Medical School, Tochigi, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Konishi
authors.forename: R
authors.initials: R
authors.lastname: Shimizu
authors.forename: L
authors.initials: L
authors.lastname: Firestone
authors.forename: F R
authors.initials: FR
authors.lastname: Walters
authors.forename: W R
authors.initials: WR
authors.lastname: Wagner
authors.forename: W J
authors.initials: WJ
authors.lastname: Federspiel
authors.forename: H
authors.initials: H
authors.lastname: Konishi
authors.forename: B G
authors.initials: BG
authors.lastname: Hattler
mesh_terms: D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D005112:Extracorporeal Circulation / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010978:Platelet Factor 4 / Q000378:metabolism; D013499:Surface Properties; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control
chemical_list: D008567:Membranes, Artificial; D009569:Nitric Oxide; D010978:Platelet Factor 4