Smoking impairs alveolar macrophage activation after inert dust exposure.
Magnetopneumography was applied to investigate intracellular phagosome motion in alveolar macrophage cells of healthy subjects (non-smokers and smokers). Ingested magnetic microparticles are inhaled and phagocytized by alveolar macrophages within hours. Thereby the particles are transferred into phagolysosomes. After magnetization the particles produce a macroscopic magnetic field of the lungs. Cellular motility causes a decay of the field (relaxation) by stochastic disorientation of the dipole particles (phagolysosomes) in the cells. Our studies have shown that the deposition of magnetite test particles induces a non-specific activation of the macrophage cells with a faster relaxation. This activation vanishes within the first day after particle deposition. This macrophage activation due to dust exposure was not present in smokers. It follows that cigarette smoking either causes a damage of the cellular defense or causes an adaptation of the macrophage cells to the permanent cigarette smoke inhalation.
Möller
W
W
GSF-Institut für Inhalationsbiologie, Oberschleissheim, Germany.
Barth
W
W
Pohlit
W
W
Rust
M
M
Siekmeier
R
R
Stahlhofen
W
W
Heyder
J
J
D000280:Administration, Inhalation; D002465:Cell Movement / Q000187:drug effects; D004391:Dust / Q000009:adverse effects*; D005290:Ferric Compounds / Q000008:administration & dosage; D006801:Humans; D008168:Lung / Q000166:cytology / Q000502:physiology; D008262:Macrophage Activation / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000502:physiology*; D008280:Magnetics; D010588:Phagosomes / Q000187:drug effects / Q000502:physiology*; D012907:Smoking / Q000009:adverse effects*
D004391:Dust; D005290:Ferric Compounds; C001215:ferrite