Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5.
A novel gene (GOK) has been cloned from human chromosome region 11p15.5 that is believed to contain a gene or genes associated with a number of pediatric malignancies, including Wilms tumor. A 4-kb cDNA has been cloned and it encodes a predicted protein of approximately 84 kDa that could be translated in vitro. Computer analysis predicted that the protein had a signal peptide and may contain a transmembrane helix. Restriction mapping by pulsed-field electrophoresis indicates that GOK is located 1.7 kb telomeric of RRM1, and both genes are transcribed in the same direction. GOK displays high evolutionary conservation: cloning and partial sequencing of a mouse genomic clone revealed 90% identity with the human gene at both the nucleotide and the predicted amino acid levels.
Parker
N J
NJ
Department of Haematology and Oncology, Royal Children's Hospital, Parkville, Victoria, Australia. parkern@cryptic.rch.unimelb.edu.au
Begley
C G
CG
Smith
P J
PJ
Fox
R M
RM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D000071737:Stromal Interaction Molecule 1
D018076:DNA, Complementary; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011506:Proteins; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1