[Selection of retroviral vector producing cell lines and gene transfer into hematopoietic cells].
Transduction and expression of a transgene in hematopoietic stem cells with retroviral vectors still remain major challenges for gene therapy in blood disorders. Use of an easily detectable gene marker, such as the nlsLacZ, at the laboratory and clinical levels, provides a powerful approach of these two combined problems.
Bagnis
C
C
IPC 232, Marseille, France.
Mannoni
P
P
D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D015316:Genetic Therapy; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells; D006801:Humans; D012190:Retroviridae
D005819:Genetic Markers