Generation of hydroxyl radicals by polymorphonuclear neutrophils during exposure to direct current and iron.
Human polymorphonuclear neutrophils were exposed to direct output current of 3 or 5 mA at either 300 or 500 V for 1 h in the presence and absence of iron. The current density was 25 or 40 nA/cm2. The formation of free oxygen radicals was measured as breakdown products from deoxyribose and methional. The cells were shown to generate oxygen radicals on electrical stimulation. A physiological concentration of iron enhanced radical formation, but OH. was also formed in the absence of added iron. The most pronounced stimulation was seen at output current 5 mA and 500 V.
Fagerheim
I
I
Department of Hospital Infection Control, Rikshospitalet, Oslo, Norway.
Lingaas
E
E
D002478:Cells, Cultured; D003855:Deoxyribose / Q000378:metabolism; D004558:Electric Stimulation; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D009504:Neutrophils / Q000502:physiology*; D010084:Oxidation-Reduction; D010649:Phenylalanine / Q000737:chemistry
D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D010649:Phenylalanine; D003855:Deoxyribose; D007501:Iron