title: Crucial domains are conserved in Enterobacteriaceae porins.
abstract: With the recent resolution of the crystal structures of several bacterial porins, it is worthwhile to define the generality of their organization throughout the Enterobacteriaceae. The distribution of specific epitopes was analysed among various Gram-negative bacterial porins using anti-peptide antibodies specific to exposed, transmembrane spanning, or pore-forming regions of Escherichia coli porins. Anti-peptide antibodies which recognized the exposed epitopes indicated a great variability among the bacterial porins analysed. Interestingly, an antigenic site located in the internal loop L3 constricting the pore diameter was present in the majority of the bacterial porins tested. Two epitopes located in domains involved in subunit interaction were also highly conserved. The presence of these common peptides suggested a conservation of specific regions involved in the functional organization of the enterobacterial porins.
authors.affiliation: Enveloppe et permeation chez les Enterobacteries, France.
authors.forename: V
authors.initials: V
authors.lastname: Simonet
authors.forename: M
authors.initials: M
authors.lastname: Mallea
authors.forename: D
authors.initials: D
authors.lastname: Fourel
authors.forename: J M
authors.initials: JM
authors.lastname: Bolla
authors.forename: J M
authors.initials: JM
authors.lastname: Pages
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics; D017124:Conserved Sequence; D003429:Cross Reactions; D004755:Enterobacteriaceae / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000276:immunology; D007120:Immunochemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D018272:Porins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D018272:Porins