title: Immunization with plasmid DNA encoding for the measles virus hemagglutinin and nucleoprotein leads to humoral and cell-mediated immunity.
abstract: We have evaluated the DNA vaccination strategy for measles virus (MV) hemagglutinin (HA) and nucleoprotein (NP) genes. Plasmids encoding either the MV, HA, or NP proteins inoculated intramuscularly into Balb/c mice induced both humoral and CTL class I restricted responses. Antibody responses were not increased by multiple inoculations. The major antibody isotype induced by both the HA and NP was IgG2a consistent with a Th1 response. In contrast, immunization with a plasmid which directed the synthesis of a partially secreted form of HA gave mainly IgG1 antibodies. When the amount of DNA was reduced for the HA plasmid (1 or 10 microg/animal), although the antibody was not induced, a CTL response was observed.
authors.affiliation: INSERM Unit 404 Immunity and Vaccination, Institut Pasteur de Lyon, France.
authors.forename: A I
authors.initials: AI
authors.lastname: Cardoso
authors.forename: M
authors.initials: M
authors.lastname: Blixenkrone-Moller
authors.forename: J
authors.initials: J
authors.lastname: Fayolle
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Buckland
authors.forename: T F
authors.initials: TF
authors.lastname: Wild
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D005260:Female; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008459:Measles virus / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics* / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C032397:hemagglutinin protein G, measles virus; C058314:nucleoprotein, Measles virus