title: Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.
abstract: A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broad host range mob+ plasmids containing gfp expressed from a lac and an npt2 promoter, respectively.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Matthysse
authors.forename: S
authors.initials: S
authors.lastname: Stretton
authors.forename: C
authors.initials: C
authors.lastname: Dandie
authors.forename: N C
authors.initials: NC
authors.lastname: McClure
authors.forename: A E
authors.initials: AE
authors.lastname: Goodman
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins