title: A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria.
abstract: In vitro root canal dentinal tubule invasion by selected anaerobic bacteria commonly isolated from endodontic infections was evaluated. Dentinal cylinders obtained from bovine incisors were inoculated with bacteria, and microbial penetration into tubules was demonstrated by scanning electron microscopy. The results indicated that all bacterial strains tested were able to penetrate into dentinal tubules, but to different extents.
authors.affiliation: Department of Postgraduate Endodontics, Gama Filho School of Dentistry, Rio de Janeiro, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: M
authors.initials: M
authors.lastname: De Uzeda
authors.forename: M E
authors.initials: ME
authors.lastname: Fonseca
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification; D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D002417:Cattle; D003786:Dental Pulp Cavity / Q000382:microbiology* / Q000648:ultrastructure; D003804:Dentin / Q000382:microbiology* / Q000648:ultrastructure; D013293:Enterococcus faecalis / Q000302:isolation & purification; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D017861:Porphyromonas / Q000302:isolation & purification; D011424:Propionibacterium / Q000302:isolation & purification; D014092:Tooth Root / Q000382:microbiology* / Q000648:ultrastructure