title: Mixed disulphide formation in myoglobin of mouse (Mus musculus).
abstract: The myoglobin, isolated from murine skeletal muscles by chromatofocusing, showed the three components, one major and two minor, with different electrophoretic mobilities. The major component with the isoelectric point (pI) of 7.55 had one reactive SH/mole, while the others with pI of 7.32 and 7.16 showed none, which could be rendered fully reactive by treating the proteins with beta-mercaptoethanol. The three components were the same in their molecular weight (18 kDa), amino-acid composition with one Cys residue and oxygenation properties. By a sensitive high-performance liquid chromatography method, the occurrence of cysteine or glutathione mixed disulphide was verified in the two minor components. We conclude from these results and incubation experiments with low-molecular-weight thiols that the two minors were derived from the major by a mixed disulphide formation with either cysteine or glutathione of the cysteinyl SH at the 66th sequence.
authors.affiliation: Second Department of Physiology, Nara Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Enoki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohga
authors.forename: H
authors.initials: H
authors.lastname: Ishidate
authors.forename: S
authors.initials: S
authors.lastname: Sakata
authors.forename: H
authors.initials: H
authors.lastname: Kohzuki
authors.forename: M
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR / Q000097:blood*; D008970:Molecular Weight; D009211:Myoglobin / Q000737:chemistry*; D010100:Oxygen / Q000097:blood; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D017186:Titrimetry
chemical_list: D004220:Disulfides; D009211:Myoglobin; D013438:Sulfhydryl Compounds; D010100:Oxygen