Systematic sequencing of the human HLA-A/HLA-F region: establishment of a cosmid contig and identification of a new gene cluster within 37 kb of sequence.
The class I region of the human histocompatibility complex is characterized by a high density of genes and pseudogenes and a complex structural organization. To elucidate the complete structure of the HLA-A/HLA-F region with a view to defining its contents in genes and pseudogenes, we developed a strategy of systematic sequencing. This report describes the establishment of a cosmid contig spanning most of the region and the analysis of a 37-kb sequence from one of the cosmids. Four new genes, organized with the HCG-V gene in a clustered structure, have been identified. Two of these contain a zinc finger motif characteristic of DNA-binding proteins. The former, a member of the C3HC4 protein family, is highly expressed in prostate and contains a B30-2-like sequence identified in several genes mapped within the class I region. The latter, which is ubiquitously expressed, is the human equivalent of the yeast polymerase IA12.2 subunit and of the murine tctex6 gene. Of the two other genes, one remains an anonymous gene with no particular feature, while the fourth, specifically expressed in testis, is the human equivalent of the murine tctex4 gene. This cluster, located in a region corresponding to a syntenic unit between mouse and human, appears to be highly conserved.
Lepourcelet
M
M
Laboratoire de Biochimie et Biologie Moléculaire, UPR 41 CNRS, Faculté de Médecine, Rennes, France.
Andrieux
N
N
Giffon
T
T
Pichon
L
L
Hampe
A
A
Galibert
F
F
Mosser
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003360:Cosmids / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004398:Dyneins; D019143:Evolution, Molecular; D005796:Genes; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D006432:Hemochromatosis / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D013737:Testis / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers / Q000235:genetics; D056427:t-Complex Genome Region
D004268:DNA-Binding Proteins; D006680:HLA Antigens; D015234:HLA-A Antigens; C062341:HLA-F antigens; D015395:Histocompatibility Antigens Class I; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C533389:Tcte3 protein, mouse; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases; D004398:Dyneins