Detection of DSP-toxins, okadaic acid, and dinophysis toxin-1 in shellfish by serine/threonine protein phosphatase assay.
A relatively rapid protein phosphatase-based assay was developed for detecting okadaic acid in extracts of shellfish, using oysters (Crassostrea virginica) as a model. The assay has good sensitivity, detecting okadaic acid in crude methanolic extracts of oysters at > or = 4 ng/mL (> or = 0.1 ng/assay). Assay accuracy for detecting toxic shellfish was validated through a series of spike recovery experiments. In more than 320 assessments, all oysters containing toxic amounts of okadaic acid (> or = 0.2 microgram/g) were detected. Results of analysis of the same extract by phosphatase assay and liquid chromatography gave very high correlation.
Honkanen
R E
RE
University of South Alabama, College of Medicine, Department of Biochemistry and Molecular Biology, Mobile 36688, USA.
Mowdy
D E
DE
Dickey
R W
RW
D000818:Animals; D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000378:metabolism*; D005506:Food Contamination; D008387:Marine Toxins / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D019319:Okadaic Acid / Q000032:analysis* / Q000031:analogs & derivatives; D010125:Ostreidae; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000378:metabolism; D011714:Pyrans / Q000032:analysis*; D015203:Reproducibility of Results; D012694:Serine
D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; D012694:Serine; C051904:dinophysistoxin 1; D010749:Phosphoprotein Phosphatases; D000432:Methanol