title: Chemical modification of metallic implant surfaces with biofunctional proteins (Part 1). Molecular structure and biological activity of a modified NiTi alloy surface.
abstract: Human plasma fibronectin (pFN), an adhesive protein, was covalently immobilized onto an alkylaminosilane derivative of a NiTi substrate with glutaraldehyde through Schiff's base formation. The surface at different stages of the modification was characterized by X-ray photoelectron spectroscopy (XPS), and the amount of immobilized pFN was determined by a fluorometric method. The spreading behavior of human gingival fibroblasts was examined on the modified surface. The XPS spectra suggested that gamma-aminopropyltriethoxysilane (gamma-APS) was bonded to the surface through metallosiloxane bonds (Ti-O-Si) formed via a condensation reaction between the silanol end of gamma-APS and the surface hydroxyl group, with a highly cross-linked siloxane network formed after heat treatment of the silanized surface at 100 degrees C. The pFN was immobilized at the surface density of 1.1 micrograms.cm-2, and significantly promoted fibroblast spreading, suggesting that this chemical modification offers an effective means of controlling metal/cell interactions. These results may contribute to the development of bioactive metallic implants.
authors.affiliation: Department of Dental Materials Science, Health Sciences University of Hokkaido, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Endo
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D003432:Cross-Linking Reagents / Q000737:chemistry; D003722:Dental Alloys / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology*; D005353:Fibronectins / Q000737:chemistry*; D005881:Gingiva / Q000166:cytology; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry*; D011437:Propylamines; D019736:Prostheses and Implants; D011485:Protein Binding; D012821:Silanes / Q000737:chemistry; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014965:X-Rays
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003722:Dental Alloys; D005353:Fibronectins; D011437:Propylamines; D012821:Silanes; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium; C477625:amino-propyl-triethoxysilane; D005976:Glutaral