title: Granulosa cells from follicle stimulating hormone-primed monkeys enhance the development competence of in-vitro-matured oocytes from non-stimulated rhesus monkeys.
abstract: The specific aims of this study were to determine if culture with freshly recovered granulosa cells obtained from follicle stimulating hormone (FSH)-primed versus non-stimulated monkeys during in-vitro maturation (IVM) improves the meiotic and development capacity of oocytes from non-stimulated macaque monkeys and confers on them competence to develop into blastocysts in vitro. Antral follicles > or = 1 mm in diameter were dissected from the excised ovaries of 11 non-stimulated rhesus monkeys. Cumulus-enclosed germinal vesicle-stage oocytes (n = 282) were randomly cultured in each of three IVM treatments; (i) control (no granulosa cells), (ii) non-stimulated granulosa cells (5 x 10(6) live cells/ml) from non-stimulated monkeys, or (iii) FSH-primed granulosa cells (5 x 10(6) live cells/ml) from monkeys primed with purified FSH for 8.5 days. All treatments contained gonadotrophins (5 micrograms/ml FSH; 10 micrograms/ml luteinizing hormone). Oocytes were cultured for 36-41 h in 25 microliters drops (one to 10 oocytes per drop) of modified CMRL-1066 medium containing 20% bovine calf serum, and then inseminated and cultured in the same medium (minus gonadotrophins) until developmental arrest or zona escape. Nuclear maturation, fertilization and cleavage to the 8-cell stage did not differ for the three treatment methods. However, development to the 9- to 15-cell stage was improved (P < or = 0.01) by culture with either non-stimulated granulosa or FSH-primed granulosa cells compared with control. The culture of oocytes with FSH-primed granulosa but not non-stimulated granulosa cells enhanced (P < or = 0.05) development to the morula stage compared with control (23, 11 and 5% respectively). Oocytes cultured with FSH-primed granulosa cells were more (P < or = 0.05) competent to develop into blastocysts (6.5%) than controls (0.5%). In conclusion, the culture of immature oocytes from non-stimulated monkeys with fresh granulosa cells recovered from FSH-primed monkeys enhanced their competence to develop into morulae and blastocysts in vitro, and resulted in the first blastocysts produced in vitro from in-vitro-matured/in-vitro-fertilized oocytes of non-stimulated macaques.
authors.affiliation: Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Schramm
authors.forename: B D
authors.initials: BD
authors.lastname: Bavister
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000502:physiology*; D008253:Macaca mulatta / Q000502:physiology*; D008540:Meiosis; D009865:Oocytes / Q000502:physiology*
chemical_list: D005640:Follicle Stimulating Hormone