A new monoclonal antibody detecting ochratoxin A at the picogram level.
A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A-BSA. This antibody was of the IgG1 heavy chain subclass with a kappa type light chain. The 50% inhibition value was 0.45 ng ml-1 in a direct competitive ELISA and the detection limit was 42 pg ml-1. This antibody is very specific, cross-reacting only with ochratoxin B (9.3%).
Gyöngyösi-Horváth
A
A
Agricultural Biotechnology Center, Gödöllö, Hungary.
Barna-Vetró
I
I
Solti
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology / Q000592:standards; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology / Q000592:standards; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A