[Characterization of the MCC gene product].
MCC is a gene located within human chromosome band 5q21 that shows somatically acquired mutations in colorectal cancer. Here we report the identification, tissue specific distribution, subcellular localization, and growth regulating activity of MCC protein. Immunohistochemical analysis of MCC protein in various tissues of mouse revealed heavy cytoplasmic staining of brain neuronal cells and intestinal epithelial cells. Ectopic expression of the MCC gene by microinjection in normal NIH3T3 cell lines leads to inhibition of DNA synthesis. These studies provide some initial clues to the function of the MCC protein and demonstrate that it negatively controls the cell cycle progression.
Matsumine
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Department of Oncogene Research, Osaka University.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002453:Cell Cycle; D003110:Colonic Neoplasms / Q000235:genetics; D017431:Genes, MCC; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D009154:Mutation; D011506:Proteins / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins
D011506:Proteins; D025521:Tumor Suppressor Proteins; C067857:MCC protein, human