A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns.
We cloned two novel types of TEA domain-containing transcription factor (ETFR-1 and -2) cDNAs. Amino acid sequences deduced for ETFR-1 and -2 as well as those of other known proteins of the same family, TEF-1 and ETF, exhibited significant identity (63-75% overall) to each other not only in the TEA DNA binding domain but also in the C-terminal regions. Northern blot analysis revealed that both the mRNAs were expressed in embryos as well as in many adult tissues, although their levels of expression varied. The results demonstrate that the mammalian TEA domain-containing transcription factor family consists of at least four distinct members, TEF-1, ETF, ETFR-1, and ETFR-2, which exhibit overlapping but differing spatiotemporal expression patterns, suggesting their redundant yet unique roles involved in not only developmental control but also tissue-specific regulation.
Yasunami
M
M
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan.
Suzuki
K
K
Ohkubo
H
H
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry*; D004330:Drosophila; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008322:Mammals; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D009687:Nuclear Proteins; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000091262:TEA Domain Transcription Factors; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000091262:TEA Domain Transcription Factors; C057182:TEAD1 protein, human; C486309:TEAD4 protein, human; C100498:TEFR1 protein, isoform b, mouse; C432156:Tead3 protein, mouse; C101204:Tead4 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors