Characterization of 42 highly polymorphic bovine microsatellite markers.
We have isolated 42 highly polymorphic microsatellite (GT/CA)n markers from Japanese black cattle Wagyu (Bos taurus). Forty-one of the markers were assigned to bovine autosomes with lod scores > 6, through linkage analyses performed on the International Bovine Reference Family Panel (IBRP). The remaining marker showed X-linked inheritance. These markers exhibited an average heterozygosity value of 0.67 with between four and 17 alleles on the IBRP.
Hirano
T
T
Shirakawa Institute of Animal Genetics Livestock Technology Association of Japan, Fukushima, Japan.
Nakane
S
S
Mizoshita
K
K
Yamakuchi
H
H
Inoue-Murayama
M
M
Watanabe
T
T
Barendse
W
W
Sugimoto
Y
Y
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014960:X Chromosome
D017931:DNA Primers; D005819:Genetic Markers