Determination of polycyclic aromatic hydrocarbons in edible oils and fats by on-line donor-acceptor complex chromatography and high-performance liquid chromatography with fluorescence detection.
Various off-line methods for clean-up and sample enrichment are available for the analysis of polycyclic aromatic hydrocarbons (PAHs) in edible oils and fats. These methods consist of laborious and time consuming procedures. This study reports an on-line method using LC-LC coupling. After clean-up of the sample on a donor-acceptor complex chromatography (DACC) column the PAHs are transferred to and separated on an analytical HPLC column. Quantification is carried out with fluorescence detection. The DACC column clean-up is fast and is carried out during the HPLC run of the previous sample. Compared to the traditional methods this automated on-line method saves considerable time and significantly reduces the amount of solvent waste. The method uses common HPLC equipment and its performance has been evaluated.
van Stijn
F
F
Unilever Research Laboratorium, Vlaardingen, Netherlands.
Kerkhoff
M A
MA
Vandeginste
B G
BG
D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D016001:Confidence Intervals; D004042:Dietary Fats, Unsaturated / Q000032:analysis; D005224:Fats, Unsaturated / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D000069463:Olive Oil; D009862:Online Systems / Q000295:instrumentation*; D010938:Plant Oils / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D002273:Carcinogens; D004042:Dietary Fats, Unsaturated; D005224:Fats, Unsaturated; D000069463:Olive Oil; D010938:Plant Oils; D011084:Polycyclic Aromatic Hydrocarbons