Structural organization of the rae28 gene, a putative murine homologue of the Drosophila polyhomeotic gene.
A putative murine homologue of the Drosophila polyhomeotic gene, named rae28, has been isolated from a genomic library of 129/SV mouse and its structural organization has been analyzed. rae28 is a single gene of approximately 22 kb long and consists of 15 exons. Its 5'-flanking region lacks typical transcriptional regulatory sequences, such as TATA and CCAAT boxes, but contains GC-rich sequences and seven putative binding sites for a transcription factor, Sp1. One major transcription start point has been determined. The overall exon-intron organization suggested that three different Rae28 mRNAs are generated through alternative splicing. Furthermore, the rae28 gene has been located on the R-positive F3 band of mouse chromosome 6 by the direct R-banding fluorescence in situ hybridization methods.
Abdul Motaleb
M
M
Department of Medical Genetics, Osaka University.
Takihara
Y
Y
Nomura
M
M
Matsuda
Y
Y
Higashinakagawa
T
T
Shimada
K
K
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005091:Exons; D017344:Genes, Insect; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D063150:Polycomb Repressive Complex 1; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009698:Nucleoproteins; C072820:ph-d protein, Drosophila; D063150:Polycomb Repressive Complex 1