title: An efficient DDAB-mediated transfection of Drosophila S2 cells.
abstract: I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.
authors.affiliation: Department of Genetic Engineering, Hallym University, Chunchon, Republic of Korea. khhan@sun.hallym.ac.kr
authors.forename: K
authors.initials: K
authors.lastname: Han
mesh_terms: D000818:Animals; D002460:Cell Line; D004330:Drosophila; D017930:Genes, Reporter; D007763:Lac Operon; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D014162:Transfection
chemical_list: D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium