title: Pericellular pO2 as an alternative method to test cytotoxicity.
abstract: Pericellular pO2 (ppcO2) was compared with the release of cytoplasmatic enzyme lactate dehydrogenase (LDH) and mitochondrial metabolic function (tetrazolium salt reduction, MTT) as an alternative method to evaluate cytotoxicity. L-929 cells were seeded and incubated with 3 different control materials to test their cytotoxicity effects by these methods. High density polyethylene and copper were respectively used as negative and positive controls, while a 0.9% NaCl solution was as reagent control and extraction vehicle. PpcO2 was measured by a rod polarographic Clark-type probe connected to Licox pO2 computer (GMS mbH, Kiel, Germany). One-way ANOVA test showed significant differences among groups regarding every methods (p < 0.0005). The comparison of the mean variation coefficients of three methods showed no significant differences. In addition, a significant correlation was found between ppcO2 and MTT (r = 0.621; p < 0.001) as well as ppcO2 and LDH data (r = 0.474; p < 0.001). In conclusion, ppcO2 may be considered a reliable, safely and alternative method to test cytotoxicity.
authors.affiliation: Servizio di Chirurgia Sperimentale, Istituto di Ricerca Codivilla-Putti, Istituto Ortopedico Rizzoli, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: P
authors.initials: P
authors.lastname: Torricelli
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: R
authors.initials: R
authors.lastname: Giardino
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis*; D011048:Polarography / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D013778:Tetrazolium Salts; D007770:L-Lactate Dehydrogenase; D010100:Oxygen