title: A Mycobacterium tuberculosis gene cluster encoding proteins of a phosphate transporter homologous to the Escherichia coli Pst system.
abstract: We report the cloning and sequencing of three M. tuberculosis genes encoding proteins homologous to E. coli PstA, PstC and PstB. They are tentatively called pstA-2, pstC-1 and pstB. They encode proteins of 302, 336 and 275 amino acids, respectively. In E. coli, PstB is the ATP binding component and PstA/PstC are the two hydrophobic subunits of a phosphate permease belonging to the family of ABC (ATP-binding cassette) transporters. In mycobacteria, PstS-1, the phosphate binding subunit (Andersen and Hansen, 1989), is encoded by a gene directly surrounded by pstB, pstC-1 and pstA-2 within a potential operon (pstB, pstS-1, pstC-1, pstA-2). Phosphate uptake by whole, suspension grown, M. bovis BCG cells was measured and could be inhibited by a monoclonal antibody directed against the PstS-1 subunit, suggesting that these genes encode subunits of a functional mycobacterial phosphate permease.
authors.affiliation: Department of Virology, Institut Pasteur, Brussels/Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Braibant
authors.forename: P
authors.initials: P
authors.lastname: Lefèvre
authors.forename: L
authors.initials: L
authors.lastname: de Wit
authors.forename: P
authors.initials: P
authors.lastname: Peirs
authors.forename: J
authors.initials: J
authors.lastname: Ooms
authors.forename: K
authors.initials: K
authors.lastname: Huygen
authors.forename: A B
authors.initials: AB
authors.lastname: Andersen
authors.forename: J
authors.initials: J
authors.lastname: Content
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001692:Biological Transport; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009163:Mycobacterium bovis / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D010710:Phosphates / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D010710:Phosphates; C489729:PstA protein, E coli; C102499:PstA-2 protein, Mycobacterium tuberculosis; C489730:PstC protein, E coli; C078409:PstC protein, bacteria; C102501:PstC-1 protein, Mycobacterium tuberculosis; C078408:phosphate-specific transport protein A, bacteria