title: Fibronectin-binding protein of Streptococcus equi subsp. zooepidemicus.
abstract: By screening a genomic lambda library of Streptococcus equi subsp. zooepidemicus, we have cloned and sequenced a gene, termed fnz, encoding a fibronectin (Fn)-binding protein called FNZ. On the basis of the deduced amino acid sequence of FNZ, the mature protein has a molecular mass of approximately 61 kDa. Analysis of FNZ reveals a structural organization similar to that of other cell surface proteins from streptococci and staphylococci. The Fn-binding activity is localized to two domains in the C-terminal part of FNZ. One domain is composed of five repeats, which contain a motif similar to what has earlier been found in other Fn-binding proteins in streptococci and staphylococci. The first and second repeats are separated by a short stretch of amino acids, including the motif LAGESGET, which is an important part of the second Fn-binding domain. This motif is also present in an Fn-binding domain (UR) in protein F of Streptococcus pyogenes. A fusion protein covering the Fn-binding domain of FNZ inhibits the binding of the 29-kDa N-terminal fragment of Fn to cells of various streptococcal species as well as to Staphylococcus aureus.
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Lindmark
authors.forename: K
authors.initials: K
authors.lastname: Jacobsson
authors.forename: L
authors.initials: L
authors.lastname: Frykberg
authors.forename: B
authors.initials: B
authors.lastname: Guss
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D018502:Streptococcus equi / Q000235:genetics* / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; C078635:fibronectin-binding proteins, bacterial