Negative inotropic effect of chagasic IgG mediated by nitric oxide.
In this paper we analyse the interaction of IgG from T. cruzi infected patients with cardiac muscarinic acetylcholine receptors (mAChRs). Human chagasic IgG, activating M2 mAChR simulated the agonist actions excerting negative inotropic effect and stimulation of nitric oxide synthase (NOS). Inhibitors of phospholipase C, protein kinase C, calcium/calmodulin, NOS and guanylate cyclase activities prevented the chagasic effects upon contractility and NOS activity.
Vila Echagüe
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A
Pharmacology Department, University of Buenos Aires, Argentina.
Sterin-Borda
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L
D000818:Animals; D002217:Carbachol / Q000097:blood / Q000494:pharmacology; D014355:Chagas Disease; D006152:Cyclic GMP; D003864:Depression, Chemical; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006325:Heart Atria / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D018721:Muscarinic Agonists; D009200:Myocardial Contraction / Q000187:drug effects*; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase / Q000097:blood / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic
D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; D018721:Muscarinic Agonists; D011976:Receptors, Muscarinic; D009569:Nitric Oxide; D002217:Carbachol; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP