title: Endothelin-stimulated phosphoinositide turnover and protein kinase C translocation in rat synaptosomes.
abstract: The action of endothelin-1 (ET-1) on phosphoinositide metabolism was studied in rat synaptosomes. ET-1 caused an early and transitory decrease of 32P incorporation into phosphoinositides, concomitantly with an increase into phosphatidic acid (PA). This effect was time-dependent and was not found in the absence of exogenous calcium. Furthermore, ET-1 caused an increase in the generation of inositol phosphates and diacylglycerol (DAG). In addition, the peptide provoked a translocation of protein kinase C from the cytosol to membrane.
authors.affiliation: Departamento de Biologia Molecular, Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain.
authors.forename: R E
authors.initials: RE
authors.lastname: Catalan
authors.forename: A M
authors.initials: AM
authors.lastname: Martinez
authors.forename: M D
authors.initials: MD
authors.lastname: Aragones
authors.forename: I
authors.initials: I
authors.lastname: Fernandez
authors.forename: B G
authors.initials: BG
authors.lastname: Miguel
authors.forename: M J
authors.initials: MJ
authors.lastname: Perez
authors.forename: M C
authors.initials: MC
authors.lastname: Calcerrada
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex; D003593:Cytoplasm / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D019332:Endothelin-1; D007295:Inositol Phosphates; D010710:Phosphates; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D011493:Protein Kinase C; D002118:Calcium