title: The structure, sequence and developmental pattern of expression of the white gene in the blowfly Lucilia cuprina.
abstract: We have sequenced the complete coding region of the white gene of Lucilia cuprina. Strong sequence identity exists between this gene and its homologue from Drosophila melanogaster at both nucleotide and derived amino acid levels (68% and 78% respectively). The exon/intron structure of the two genes is also largely conserved, although the Lucilia gene contains one extra intron. Expression of the gene peaks during mid-pupal stage, with secondary peaks in late larval and early adult stages. Comparisons between this and other white genes will contribute to a better understanding of ATP-binding transmembrane transport proteins. The white gene should also serve as a useful marker gene in the development of a gene transformation system for the sheep blowfly.
authors.affiliation: Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, Australia.
authors.forename: R L
authors.initials: RL
authors.lastname: Garcia
authors.forename: H D
authors.initials: HD
authors.lastname: Perkins
authors.forename: A J
authors.initials: AJ
authors.lastname: Howells
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004175:Diptera / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018528:ATP-Binding Cassette Transporters; D018076:DNA, Complementary; D029721:Drosophila Proteins; D005136:Eye Proteins; C059254:w protein, Drosophila