A partially purified beta-glucosidase from Bifidobacterium adolescentis converts cycasin to a mutagenic compound.
beta-Glucosidase was extracted from sonicated Bifidobacterium adolescentis Int-57 and partially purified by Sepharose CL-6B gel-filtration and DEAE-cellulose ion-exchange chromatography. The partially purified enzyme was confirmed to convert cycasin to a mutagen in the Ames and SOS chromotests. beta-Glucosidase negative strains were unable to activate cycasin mutagenically.
Choi
Y J
YJ
Department of Food Science, Sook-Myung Women's University, Seoul, Korea.
Kim
C J
CJ
Ji
G E
GE
D001644:Bifidobacterium / Q000201:enzymology*; D001673:Biodegradation, Environmental; D003492:Cycasin / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D005516:Food Microbiology; D006801:Humans; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*
D009153:Mutagens; D003492:Cycasin; D001617:beta-Glucosidase