Monoclonal antibody against an amniotic protein carrying ABH blood group epitopes and its forensic application.
A mouse monoclonal antibody against an amniotic protein carrying ABH antigenic epitopes was established. BALB/c mice were immunized by an amniotic protein of molecular weight over 200 kDa, which had proved to be the carrier protein of ABH blood group epitopes by analysis with SDS-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amniotic carrier protein without affecting the ABH blood group antigenicity, and did not cross-react with other body fluids which included blood, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgG1 with a titer of 1 : 1,600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-A and -B antibodies and enzyme conjugated anti-mouse IgM. The sandwich ELISA could successfully detect the blood group of amniotic fluid in mixed body fluids.
Nagae
M
M
Department of Forensic Medicine, Tohoku University School of Medicine, Sendai.
Aoki
Y
Y
Nata
M
M
Hashiyada
M
M
Sagisaka
K
K
D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007231:Infant, Newborn; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010334:Paternity; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D011506:Proteins