Relative phosphocreatine and nucleoside triphosphate concentrations in cerebral gray and white matter measured in vivo by 31P nuclear magnetic resonance.
Rates of ATP metabolism generally are higher in cerebral gray matter compared to white matter. In order to study the physiology of this regional difference in vivo, the 1-dimensional chemical shift imaging technique (1D-CSI) was used to acquire 31P nuclear magnetic resonance spectra from 2.5 mm slices of 4-week old piglet brains. Spectra from predominantly gray matter slices (estimated 76% gray matter, 7 mm below the scalp) were compared to predominantly white matter slices (56% estimated white matter, 13 mm below the scalp) as assessed by magnetic resonance images. The 1D-CSI technique introduced no systematic changes in the ratio of signals from a single chamber phantom containing a phosphocreatine (PCr) and ATP solution. Gray matter slices showed a PCr/NTP ratio of 0.93 +/- 0.11 (mean +/- S.D.) using a 2 s interpulse interval, a value very close to the ratio in surface coil localized spectra. The predominantly white matter slices showed a PCr/NTP ratio of 1.32 +/- 0.18 (P < 0.02 for gray versus white matter). Using the estimated percentages of gray and white matter in the two slices and calculated concentrations from fully relaxed spectra, the gray matter PCr/NTP ratio is approximately 0.77, while the ratio in white matter is approximately 2.18. The difference in PCr/NTP measured in vivo suggests that either the total NTP concentration is higher or the steady state PCr concentration is lower in gray matter than in white matter in the piglet brain.
Tsuji
M K
MK
Joint Program in Neonatology, Harvard Medical School, Boston, MA, USA.
Mulkern
R V
RV
Cook
C U
CU
Meyers
R L
RL
Holtzman
D
D
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D010725:Phosphocreatine / Q000378:metabolism*; D010759:Phosphorus Isotopes; D013552:Swine; D013556:Swine, Miniature
D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D000255:Adenosine Triphosphate