Differential expression of CD44 splice variants in malignant and benign pleural effusions.
Expression of CD44 isoform that contains variant exons (v1-v10) has been implicated in tumor progression and metastasis. Especially, CD44 isoform containing v6 (CD44v6) and that containing v7 (CD44v7) were shown to confer full metastatic behavior on tumor cells. We examined the expression of CD44v6 and CD44v7 in malignant pleural effusions (13 lung cancers and 4 non-lung cancers) and in benign pleural effusions (7 tuberculosis and 3 pneumonia) with Southern blot analysis of reverse transcription (RT)-polymerase chain reaction (PCR) products. CD44v6 was expressed not only in malignant pleural effusions (12 of 13 lung cancers and 4 of 4 non-lung cancers), but also in benign pleural effusions(9/10). In contrast, although expression of CD44v7 was found in most malignant pleural effusions (12 of 13 lung cancers and 4 of 4 non-lung cancers), it was found in only a few cases of benign pleural effusions. These results suggest that the expression of CD44v7 may be correlated with a tumor-specific event such as metastasis or dissemination in malignant pleural effusion, while no such correlation can be found with CD44v6.
Tojo
N
N
Department of Laboratory Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Inase
N
N
Ichioka
M
M
Miyazato
I
I
Nara
N
N
D017398:Alternative Splicing; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D005091:Exons; D005260:Female; D014644:Genetic Variation; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics*; D007680:Kidney Neoplasms / Q000235:genetics / Q000276:immunology; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics / Q000276:immunology; D010996:Pleural Effusion / Q000235:genetics / Q000276:immunology*; D016066:Pleural Effusion, Malignant / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology
D015703:Antigens, CD; D017931:DNA Primers; D018960:Hyaluronan Receptors