Cultured airway epithelium responses to mineral particles: role of the oxidative stress.
We tested the hypothesis according to which mineral particles containing iron would be able to produce cytotoxic-ROS. We approached this problem in vitro using primary cultures of rabbit tracheal epithelial cells. The oxidizing power of three mineral particles, i.e. nemalite, chrysotile and hematite, has been evaluated for their capacity to induce lipid peroxidation, and to activate intra-cellular anti-oxidant enzymes. The results show that nemalite and chrysotile which contain Fe2+ have a strong oxidizing power, inducing an oxidative stress on airway epithelial cells, whereas hematite, the Fe3+ containing particles, is without effect.
Guilianelli
C
C
Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Universitë de Paris, France.
Baeza-Squiban
A
A
Lapart
E
E
Marano
F
F
D000818:Animals; D017632:Asbestos, Serpentine / Q000633:toxicity*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005290:Ferric Compounds / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008276:Magnesium Hydroxide / Q000633:toxicity*; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000502:physiology; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014132:Trachea / Q000166:cytology* / Q000187:drug effects
D017632:Asbestos, Serpentine; D005290:Ferric Compounds; D017382:Reactive Oxygen Species; C000499:ferric oxide; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D008276:Magnesium Hydroxide