title: Variability associated with analytical methods used to measure aflatoxin in agricultural commodities.
abstract: A total of 1019 analytical precision estimates obtained from method-performance (collaborative) studies for mycotoxins published through 1991 were sorted by type of variance measurement, type of analytical method, and type of agricultural commodity. Precision estimates for total aflatoxin were sorted into 2 precision measurements (among-laboratories and within-laboratory), 3 analytical methods (thin-layer chromatography [TLC], liquid chromatography [LC], and enzyme-linked immunosorbent assay [ELISA]), and 11 agricultural commodities. Sufficient data existed to study the analytical variability (precision) associated with 36 sorted combinations (of a possible 66). In all but one combination (within-laboratory, barley, and TLC), the variance (V) was a function of total aflatoxin concentration (C). A power function of the form V = aCb, where a and b are constants, describes the relationship between variance and aflatoxin concentration. The coefficients a and b were determined from regression analysis. When results were pooled across all agricultural commodities, LC had the lowest analytical variability while ELISA had the highest. For a given method, among-laboratories variability was approximately double the within-laboratory variability. These analytical variability estimates can be coupled with previously determined variability estimates of sampling and sample preparation to determine the performance associated with specific test procedures used to inspect agricultural commodities for aflatoxin.
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture, North Carolina State University, Raleigh 27695-7625, USA.
authors.forename: T
authors.initials: T
authors.lastname: Whitaker
authors.forename: W
authors.initials: W
authors.lastname: Horwitz
authors.forename: R
authors.initials: R
authors.lastname: Albert
authors.forename: S
authors.initials: S
authors.lastname: Nesheim
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D005506:Food Contamination; D009754:Nuts / Q000737:chemistry
chemical_list: D000348:Aflatoxins