title: Ontogeny and cellular expression of MHC and leucocyte antigens in human retina.
abstract: We have investigated the ontogeny of MHC class I, class II, CD45, and macrophage antigens in whole mounts of normal human fetal retina at 10-25 weeks gestation (WG) using monoclonal antibodies and immunogold histochemistry. MHC class I antigens were expressed on retinal vascular endothelial cells and provided a useful marker of vessel organization from 14-25 WG. Microglial cells expressed immunoreactivity to MHC class I, class II, and CD45 antigens from 10 WG (pre-vascularization) and macrophage S22 (Mac S22) antigen from 14 WG (post-vascularization), although none of the antigens tested were detected on neuronal or macroglial elements. Microglia expressing MHC, CD45, and macrophage antigens occurred in both ramified and rounded forms with no close correlation being observed between morphology and antigenicity. The numbers of immunoreactive cells labeled with each of the four markers increased steadily throughout gestation in all specimens studied. Equivalent numbers of microglia expressed MHC class I, class II, and CD45 antigens in retinae at similar gestational ages; however, our data indicate that microglia expressing Mac S22 antigen comprise approximately 40% or less of the population of MHC and CD45-immunoreactive cells during development. Topographical analyses suggest that MHC class I, class II, and CD45-positive microglia enter the retina from both the peripheral retinal margin and the optic disc from at least 10 WG; Mac S22-positive cells appear in association with the development of the retinal vasculature and enter the retina via the optic disc after 14 WG.
authors.affiliation: Department of Clinical Ophthalmology, University of Sydney, N.S.W., Australia.
authors.forename: C M
authors.initials: CM
authors.lastname: Diaz-Araya
authors.forename: J M
authors.initials: JM
authors.lastname: Provis
authors.forename: P L
authors.initials: PL
authors.lastname: Penfold
mesh_terms: D015415:Biomarkers; D002452:Cell Count; D005260:Female; D006680:HLA Antigens / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008285:Major Histocompatibility Complex / Q000276:immunology*; D017628:Microglia / Q000737:chemistry / Q000276:immunology; D011247:Pregnancy; D012160:Retina / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D006680:HLA Antigens