title: Glycosaminoglycan stratification of the juxtacanalicular tissue in normal and primary open-angle glaucoma.
abstract: OBJECTIVE
  The juxtacanalicular tissue (JCT) is the probable site of aqueous outflow resistance in normal eyes and of the increased resistance in primary open-angle glaucoma eyes (POAG). The purpose of this histochemical study was to determine the glycosaminoglycan (GAG) composition and stratification in the JCT of POAG and age-matched normal eyes.
  
  
  METHODS
  Five eyes from four normal donors and five eyes from four POAG donors (69 to 80 years of age) were analyzed. Using methods that histochemically preserve GAGs, GAG-degrading enzymes, Alcian blue staining, and real color discrimination to exclude pigment, nuclear staining and unstained areas, the type and amount of GAGs were estimated by compute-raided charge-coupled device color video image analysis. To examine GAG stratification, the JCT was segmented into three regions-anterior, middle, and posterior-to examine regional differences in GAG composition; each region was further divided into four 2-microns layers, from layer 1, adjacent to and including the endothelium of Schlemm's canal, to layer 4, to the first trabecular lamellae.
  
  
  RESULTS
  The normal GAG JCT profile was as follows: hyaluronic acid (HA), 7.78 +/- 1.23 femtograms (fg)/micron2; chondroitin sulfates (CS), 8.18 +/- 0.82 fg/micron2; dermatan sulfate, 0.29 +/- 0.18 fg/micron2; the total, 18.73 +/- 0.68 fg/micron2. In contrast, the POAG GAG JCT profile was as follows: HA 0.57 +/- 0.31 fg/micron2 (P < 0.00001), a 93% decrease; CS 13.49 +/- 0.74 fg/micron2 (P < 0.0001), a 83% increase; dermatan sulfate, 0.90 +/- 0.53 fg/micron2; and the total, 17.31 +/- 0.95 fg/micron2, an 8.2% decrease. The HA was depleted in all layers of all regions of POAG JCT.
  
  
  CONCLUSIONS
  Results indicate that the normal JCT is stratified, with HA as the predominant GAG in layers 1 and 2. The POAG JCT is depleted of HA and has an accumulation of CS, which may increase outflow resistance and, consequently, increase intraocular pressure in patients with POAG.
authors.affiliation: Laboratory for Oculo-Cerebrospinal Investigation, Children's Memorial Medical Center, Chicago, Illinois, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Knepper
authors.forename: W
authors.initials: W
authors.lastname: Goossens
authors.forename: P F
authors.initials: PF
authors.lastname: Palmberg
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000423:Alcian Blue; D000869:Anterior Eye Segment / Q000378:metabolism* / Q000473:pathology; D002924:Ciliary Body / Q000378:metabolism; D004396:Coloring Agents; D005260:Female; D005902:Glaucoma, Open-Angle / Q000378:metabolism* / Q000473:pathology; D006025:Glycosaminoglycans / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007498:Iris / Q000378:metabolism; D008297:Male
chemical_list: D004396:Coloring Agents; D006025:Glycosaminoglycans; D000423:Alcian Blue