Genomic organization and chromosomal mapping of the bovine Fas/APO-1 gene.
The cell-surface protein Fas (APO-1) is a member of the tumor necrosis factor receptor (TNFR) superfamily and transduces apoptosis following binding of Fas ligand or exposure to certain anti-Fas antibodies. We have isolated the bovine Fas (bFas) gene and determined its genomic organization and chromosomal location. Our data indicate that bFas is a single-copy gene that contains 9 exons and spans approximately 31.5 kb. The 5'-flanking region lacks conventional TATA and CCAAT elements, but contains several putative regulatory elements, including multiple copies of Sp1, AP-2, E-box, and N-box consensus sites. Linkage analysis of two (CA) dinucleotide repeat microsatellites within intron 1 and physical assignment by fluorescence in situ hybridization (FISH) placed the bFas gene on bovine chromosome 26. Collectively, these data provide a basis for understanding the regulatory mechanisms that control bFas gene expression.
Yoo
J
J
USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, NE 68933, USA.
Stone
R T
RT
Kappes
S M
SM
Toldo
S S
SS
Fries
R
R
Beattie
C W
CW
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D018900:Dinucleotide Repeats / Q000235:genetics; D005091:Exons / Q000235:genetics; D018628:Gene Dosage; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D019014:fas Receptor / Q000235:genetics*
D019014:fas Receptor; D004247:DNA