Purification and characterization of a thermostable ADP-glucose pyrophosphorylase from Thermus caldophilus GK-24.
An extremely thermostable ADP-glucose pyrophosphorylase (AGPase) has been purified from Thermus caldophilus GK-24 to homogeneity by chromatographic methods, including gel filtration and ion-exchange and affinity chromatography. The specific activity of the enzyme was enriched 134.8-fold with a recovery of 10.5%. The purified enzyme was a single band by SDS/PAGE with a molecular mass of 52 kDa. The homotetrameric structure of the native enzyme was determined by gel filtration analysis, which showed a molecular mass of 230 kDa on a Superose-12 column, indicating that the structure of the enzyme is different from the heterotetrameric structures of higher-plant AGPases. The enzyme was most active at pH 6.0. The activity was maximal at 73-78 degrees C and its half-life was 30 min at 95 degrees C. Kinetic and regulatory properties were characterized. It was found that AGPase activity could be stimulated by a number of glycolytic intermediates. Fructose 6-phosphate, fructose 1,6-bisphosphate, phenylglyoxal and glucose 6-phosphate were effective activators, of which fructose 1,6-bisphosphate was the most effective. The enzyme was inhibited by phosphate, AMP or ADP. ATP and glucose 1-phosphate gave hyperbolic-shaped rate-concentration curves in the presence or absence of activator. A remarkable aspect of the amino acid composition was the existence of the hydrophobic and Ala+Gly residues. The N-terminal and internal peptide sequences were determined and compared with known sequences of various sources. It was apparently similar to those of AGPases from other bacterial and plant sources, suggesting that the enzymes are structurally related.
Ko
J H
JH
Molecular Glycobiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology, Yusung, Taejon.
Kim
C H
CH
Lee
D S
DS
Kim
Y S
YS
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D051599:Glucose-1-Phosphate Adenylyltransferase; D006358:Hot Temperature; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013824:Thermus / Q000201:enzymology*
D046911:Macromolecular Substances; D010446:Peptide Fragments; D009713:Nucleotidyltransferases; D051599:Glucose-1-Phosphate Adenylyltransferase