A standard immunoglobulin preparation produced from bovine colostra shows antibody reactivity and neutralization activity against Shiga-like toxins and EHEC-hemolysin of Escherichia coli O157:H7.
Enterohemorrhagic Escherichia coli (EHEC) causes a variety of clinical conditions, the most important being hemorrhagic colitis and hemolytic uremic syndrome. A curative therapy of EHEC diseases is not yet feasible. This study investigates the antibody reactivity of Lactobin, a standardized immunoglobulin (Ig) preparation, obtained from the colostra of non-immunized cows. Three different batches of Lactobin exhibited equally high titers of specific antibodies against Shiga-like toxins (SLTs, verocytotoxins) and EHEC hemolysin (EHEC-Hly) produced by E. coli O157. In addition, Lactobin blocked the cytotoxic effect of SLT-I and SLT-II on Vero cell monolayers and inhibited the cytolytic effects of EHEC-Hly on human erythrocytes. Since Lactobin contains high levels of antibodies and neutralizing activity against important virulence factors of EHEC O157, this drug has potential use in the treatment of diarrhea and the prevention of EHEC-associated hemolytic uremic syndrome.
Lissner
R
R
Biotest Pharma GmbH, Dreieich, Germany.
Schmidit
H
H
Karch
H
H
D000217:Acyltransferases; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003126:Colostrum / Q000276:immunology*; D004912:Erythrocytes; D004927:Escherichia coli Infections / Q000276:immunology; D019453:Escherichia coli O157 / Q000276:immunology*; D029968:Escherichia coli Proteins; D005260:Female; D006460:Hemolysin Proteins / Q000276:immunology*; D006463:Hemolytic-Uremic Syndrome / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology*; D009500:Neutralization Tests; D011247:Pregnancy; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D012760:Shigella / Q000276:immunology*; D014709:Vero Cells
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D007074:Immunoglobulin G; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D000217:Acyltransferases; C092237:HlyC protein, E coli