Immunocytochemical localization of myotonin protein kinase on muscle from patients with congenital myotonic dystrophy.
Using a polyclonal anti myotonin-protein kinase (M-PK) antibody against synthetic M-PK peptides corresponding to part of the amino acid sequence, and the immunohistochemical analysis of indirect immunoperoxidase, we have investigated localization of M-PK on muscle from patients with congenital myotonic dystrophy. In congenital myotonic dystrophy (MD) patients, one month and 3 months old, M-PK was weakly expressed at sarcolemma of muscle fibers. In congenital MD patients from 2 to 9 years of age, M-PK was clearly expressed at sarcolemma of muscle fibers. M-PK of immature muscle is weakly expressed at sarcolemma. With aging, M-PK is clearly expressed at sarcolemma of muscle from MD patient and normal control.
Tachi
N
N
School of Health Sciences, Sapporo Medical University, Japan.
Kozuka
N
N
Ohya
K
K
Chiba
S
S
Kikuchi
K
K
D000328:Adult; D000483:Alleles; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000473:pathology; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000473:pathology; D009223:Myotonic Dystrophy / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D065636:Myotonin-Protein Kinase; D012150:Polymorphism, Restriction Fragment Length; D011494:Protein Kinases / Q000032:analysis*; D017346:Protein Serine-Threonine Kinases; D012508:Sarcolemma / Q000201:enzymology / Q000473:pathology
C585517:DMPK protein, human; D004247:DNA; D011494:Protein Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases; D015246:Deoxyribonuclease EcoRI