title: The effect of pancreatic denervation and atropine on the cholecystokinin-induced pancreatic exocrine response.
abstract: To study the influence of extrapancreatic neural and cholinergic activity on the pancreatic response to cholecystokinin (CCK), six dogs underwent creation of Herrera pancreatic fistulas, placement of Thomas gastric cannulas, and distal pancreatectomies (innervated; INN). Six additional dogs were prepared similarly, with the addition of total extrinsic pancreatic denervation (denervated; DEN). The pancreatic protein and bicarbonate response to graded 12.5 to 200 ng/kg/h CCK doses was determined for INN and DEN animals both alone and with 10 micrograms/kg/h atropine infusion. The influence of extra-pancreatic neural and cholinergic activity on secretin's potentiation of the CCK-induced pancreatic response was then determined by repeating the studies with a 125 ng/kg/h secretin infusion. The latter results were compared to those predicted by summating the responses seen during separate 12.5-200 ng/kg/h CCK dose-response and 125 ng/kg/h secretin studies. Unstimulated protein output was diminished by atropine in INN animals (78 +/- 21 vs. 39 +/- 9 mg/15 min; p < 0.05) but not in DEN animals. Unstimulated bicarbonate outputs, integrated bicarbonate and protein outputs, and bicarbonate and protein dose-response curves were unaffected by denervation or atropine. Potentiation of CCK-induced bicarbonate output by secretin was also unaffected by atropine and denervation. We conclude that cholinergic elements are involved in unstimulated, but not CCK-induced, enzyme secretion. Further, potentiation of CCK-induced bicarbonate output by secretin does not depend on extrinsic neural or cholinergic elements.
authors.affiliation: Department of Surgery, University of Cincinnati Medical Center, Ohio, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Berry
authors.forename: B W
authors.initials: BW
authors.lastname: Kuvshinoff
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
authors.forename: A S
authors.initials: AS
authors.lastname: Fink
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002766:Cholecystokinin / Q000494:pharmacology*; D003714:Denervation; D004285:Dogs; D010179:Pancreas / Q000294:innervation / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D010276:Parasympatholytics / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*
chemical_list: D010276:Parasympatholytics; D011506:Proteins; D001285:Atropine; D002766:Cholecystokinin