title: Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I.
abstract: We have cloned the cDNA encoding the second largest subunit of RNA polymerase I, termed RPA2, from mouse cells. The cDNA has a 3978-nucleotide open reading frame encoding a polypeptide of 1136 amino acids with a calculated molecular mass of 128 kDa. A sequence alignment of mouse RPA2 with the corresponding gene from Drosophila melanogaster and yeast reveals a much lower sequence similarity of this subunit of RNA polymerase I (Pol I) compared to the second largest subunit of other eukaryotic RNA polymerases. Four Pol I-specific regions, termed I alpha-I delta, are conserved in the N-terminal part of RPA2. The structural features of the different domains as well as the homology to essential functional domains found in other RNA polymerases are discussed.
authors.affiliation: Division of Molecular Biology of the Cell II, German Cancer Research Center, Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Seither
authors.forename: I
authors.initials: I
authors.lastname: Grummt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012318:RNA Polymerase I / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D012318:RNA Polymerase I