title: Intracerebroventricular administration of beta-endorphin increases the expression of c-fos and of corticotropin-releasing factor messenger ribonucleic acid in the paraventricular nucleus of the rat.
abstract: We evaluated the effects of intracerebroventricular (i.c.v.) administration of beta-endorphin and naloxone, an opioid antagonist, on the induction of c-fos and corticotropin-releasing factor (CRF) mRNA to clarify the effects of beta-endorphin on cellular activity and CRF gene expression in the paraventricular nucleus (PVN) of the rat using in situ hybridization. A significant induction of c-fos mRNA was noted in the PVN after i.c.v. injection of beta-endorphin, compared to control. This induction was inhibited by the administration of naloxone. A significant increase in CRF mRNA levels in the PVN was observed 120 min after the i.c.v. injection of beta-endorphin. This increase was partially, but significantly, inhibited by naloxone administration. In addition, i.c.v. administration of beta-endorphin increased plasma ACTH concentration in freely moving rats, which was inhibited by intravenous injection of CRF antiserum. These results suggest that the i.c.v. injection of beta-endorphin increases the neuronal activity and the biosynthesis of CRF in the PVN, and stimulates the secretion of ACTH by increasing CRF secretion. This effect on the PVN was mediated, at least in part, via the opioid receptor.
authors.affiliation: Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College, Japan.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Imaki
authors.forename: T
authors.initials: T
authors.lastname: Shibasaki
authors.forename: N
authors.initials: N
authors.lastname: Yamauchi
authors.forename: H
authors.initials: H
authors.lastname: Demura
mesh_terms: D000818:Animals; D001345:Autoradiography; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D003720:Densitometry; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D017403:In Situ Hybridization; D007276:Injections, Intraventricular; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D001615:beta-Endorphin / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D009292:Narcotic Antagonists; D012333:RNA, Messenger; D009270:Naloxone; D001615:beta-Endorphin; D003346:Corticotropin-Releasing Hormone