Individual housing from rearing modifies the performance of young rats on the elevated plus-maze apparatus.
Previous studies from our laboratory have demonstrated that male and female rats exhibit a differential pattern of behavior in the elevated plus-maze as a function of age. In the present study, the influence of individual housing conditions on young animals treated with one of two classical anxioselective drugs, diazepam or pentylenetetrazole, was investigated in the elevated plus-maze. In Experiment I, males and females were housed for 30 days after weaning either individually or in groups, and tested in the elevated plus-maze at 60 days of age. In Experiment 2, the effects of diazepam (0.75 or 1.0 mg/kg) or of pentylenetetrazole (20 or 30 mg/kg) on the behavior of isolated or grouped rats were studied at 60 days of age in the elevated plus-maze. The results show that isolated housed animals tested with diazepam at 60 days of age exhibited increased frequency and time spent on the open arms of the apparatus compared to control rats. The effect of diazepam was not observed in grouped animals tested at 60 days of age. Pentylenetetrazole produced a decrease in the frequency and time spent on the open arms. This effect was more prominent in grouped animals. The results suggest that 60-day-old rats deprived of playfighting experience present high basal anxiety levels and also that rearing conditions (isolated or grouped) are able to interact with both anxiolytic and anxiogenic effects of experimental drugs.
Da Silva
N L
NL
Departmento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Ferreira
V M
VM
Carobrez
A de P
Ade P
Morato
G S
GS
D000818:Animals; D003975:Diazepam / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D012934:Social Isolation
D003975:Diazepam