Effects of high dose progestogens on white cells and necrosis in human endometrium.
Endometrium was collected from 20 high-dose progestogen-treated patients and examined for leukocyte populations by immunohistochemistry and phloxine-tartrazine staining. A labelled streptavidin-biotin-alkaline phosphatase technique was used with antibodies against leukocyte common antigen (LCA), T cells (CD3), neutrophils (NP57), macrophages] (CD68, KP1) and B cells (CD20). The numbers of LCA (1070 +/- 117/mm2), CD3 (459 +/- 60/mm2), CD68 (129 +/- 21/mm2) positive cells and endometrial granulated lymphocytes (EGL) (236 +/- 41/mm2) were significantly higher than those in the control group (P < 0.001). Of these, EGL increased most (6.7 times). NP57 positive (NP57+) neutrophils were present in five out of 20 progestogen-treated samples and NP57 negative (NP57-) neutrophils in another six out of 20; while a neutrophil was only identified in one control tissue (P = 0.002). Three progestogen-exposed endometrial samples had either focal or extensive necrosis, and many NP57+ and NP57- neutrophils were present in the necrotic areas. EGL, neutrophils and macrophages are known to release a number of cytolytic and cell toxic molecules which may play a role in the initiation or acceleration of progestational endometrial necrosis.
Song
J Y
JY
Department of Obstetrics and Gynaecology, University of Sydney, NSW, Australia.
Russell
P
P
Markham
R
R
Manconi
F
F
Fraser
I S
IS
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000187:drug effects* / Q000276:immunology / Q000473:pathology*; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017493:Leukocyte Common Antigens / Q000032:analysis; D019272:Leukocyte Elastase / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology*; D009336:Necrosis; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D011372:Progestins / Q000494:pharmacology*; D013194:Staining and Labeling; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D017493:Leukocyte Common Antigens; D019272:Leukocyte Elastase; D011372:Progestins; C067980:CD68 antigen, human; D000097382:CD68 Molecule