Continuous production of soluble extracellular domain of a type-I transmembrane protein in mammalian cells using an Epstein-Barr virus Ori-P-based expression vector.
Chen
Y T
YT
Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, California 94305-5426, USA. etchen@cmgm.stanford.edu
Nelson
W J
WJ
D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D018741:Replication Origin; D012995:Solubility
D015820:Cadherins; D018076:DNA, Complementary; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins