Toxicity of camphorated phenol and camphorated parachlorophenol in dental pulp cell culture.
The toxicity of phenol, parachlorophenol, camphorated phenol, camphorated parachlorophenol, and camphor was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay on established rat dental pulp cells (RPC-C2A). RPC-C2A cells at the confluent stage were incubated for 24 h in an experimental medium containing each compound at different concentrations. All tested drugs showed cytotoxicity in the MTT assay in a concentration-dependent manner. It is believed that camphor is a vehicle, and it reduces the toxicity of phenol and parachlorophenol. However, camphor itself showed cytotoxicity, and the addition of camphor increased the toxicity of phenol and parachlorophenol, reconfirming the cytotoxicity of these classical antiseptics.
Soekanto
A
A
Faculty of Dentistry, University of Indonesia, Yushima, Japan.
Kasugai
S
S
Mataki
S
S
Ohya
K
K
Ogura
H
H
D000704:Analysis of Variance; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D002164:Camphor / Q000633:toxicity*; D002417:Cattle; D002478:Cells, Cultured; D002733:Chlorophenols / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004338:Drug Combinations; D019800:Phenol; D010636:Phenols / Q000633:toxicity*; D051381:Rats; D012388:Root Canal Irrigants / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004338:Drug Combinations; D010636:Phenols; D012388:Root Canal Irrigants; D019800:Phenol; C029107:4-chlorophenol; D002164:Camphor; C102651:camphorated phenol; C029804:camphorated parachlorophenol