Lactate production by white adipocytes in relation to insulin sensitivity.
Besides its lipogenic and lipolytic functions, adipose tissue has been recognized as an important site of lactate production by epididymal adipocytes under experimental conditions in which insulin sensitivity was increased (cold exposure) or reduced (glucose perfusion). Insulin sensitivity of adipocytes was assessed by its antilipolytic effect and its stimulation of glucose uptake. We showed that, in adipocytes isolated from control and cold-exposed animals, glycerol and lactate production rose simultaneously, in response to norepinephrine. On the other hand, in adipocytes from glucose-perfused animals, we observed a dissociation between lipolytic and glycolytic responses. Indeed, lactate production in response to norepinephrine reached 60% of maximal response (10(-7)M) while the lipolytic response was still basal. This phenomenon was the result of a desensitization of beta-adrenergic receptors. However, the sensitivity of adipocytes to stimulation of lactate production by catecholamines was not different between control and glucose-perfused rats. Because lactate production in response to norepinephrine was not affected by desensitization, it suggests that lactate production is under alpha 1-adrenergic control. We confirmed this hypothesis.
Faintrenie
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G
Unité de Recherche Associée D1341-Centre National de la Recherche Scientifique Laboratoire de Thermorégulation et Energétique de l'Exercice, Lyon, France.
Géloën
A
A
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003080:Cold Temperature; D005947:Glucose / Q000493:pharmacokinetics / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D019344:Lactic Acid / Q000097:blood / Q000378:metabolism*; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D001786:Blood Glucose; D007328:Insulin; D010656:Phenylephrine; D019344:Lactic Acid; D005947:Glucose; D007545:Isoproterenol; D009638:Norepinephrine