Mass spectrometric quantification of neuropeptides.
The molecular specificity of MS/MS methods for the accurate quantification of endogenous neuropeptides, such as ME and BE, exceeds that of all other methods because: 1. MS/MS qualitative analysis first establishes the amino acid sequence of the endogenous peptide; 2. MS/MS establishes and maintains the structural link between the (M + H)+ ion and its corresponding amino acid sequence-determining ion(s) during quantification; and 3. A stable isotope-incorporated synthetic peptide internal standard is used. Endogenous ME and BE have been measured in human pituitary tissue by this quantitative analytical MS method.
Desiderio
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Charles B. Stout Neuroscience Mass Spectrometry Laboratory, Department of Neurology, University of Tennessee, Memphis, USA.
D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis*; D010902:Pituitary Gland / Q000737:chemistry*
D009479:Neuropeptides