Isolation and characterization of a putative collagen gene from the potato cyst nematode Globodera pallida.
A cDNA clone encoding a full length putative collagen has been isolated in a screen of a mixed stage Globodera pallida expression library. Comparison of the deduced amino acid sequence of this molecule with other collagens suggests it is a cuticular collagen and a member of the col-8 subfamily of collagen genes. Northern blots show the gene is expressed specifically in gravid, adult females of the parasite as compared to second (invasive) stage juveniles and virgin females. Preliminary immunocytochemical studies indicate this collagen is present in areas other than the cuticle; these findings and the potential functional role of this collagen are discussed.
Jones
J T
JT
Unit of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland. jjones@scri.sari.ac.uk
Curtis
R H
RH
Wightman
P J
PJ
Burrows
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003094:Collagen / Q000737:chemistry / Q000235:genetics*; D018509:DNA, Helminth / Q000032:analysis / Q000737:chemistry; D005260:Female; D015723:Gene Library; D017238:Genes, Helminth; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009348:Nematoda / Q000737:chemistry / Q000235:genetics*; D018510:RNA, Helminth / Q000032:analysis / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D017421:Sequence Analysis; D011198:Solanum tuberosum / Q000469:parasitology*
D018509:DNA, Helminth; D018510:RNA, Helminth; D003094:Collagen