Manipulation of the helper T cell response to influence antigenic competition occurring with a multivalent vaccine.
The reduction in antibody observed following inoculation with multiple heterologous Dichelobacter nodosus pili antigens is thought to be due to competition between antigen-specific B cells for a limited amount of T cell help. We demonstrate here that this competition is not further influenced by the expansion of cross-reactive antibody secreting cells at the expense of serogroup specific antibody secreting cells. The T cell determinants of pili recognized by sheep and BALB/c mice have been defined using 15 residue peptides. These T cell determinants include cross-reactive determinants in the conserved amino terminal region of the antigen. Here we investigate the effect of expanding the pili-specific T cell population by priming with pili derived T cell determinants. It was not possible to increase the antibody elicited in response to the multivalent vaccine by priming mice with either a synthetic peptide spanning a T cell determinant or with reduced and alkylated or heterologous serogroups of pili 4 weeks before inoculation with the multivalent vaccine. A strategy designed to increase the T cell population by inoculating animals with pili covalently coupled to an extrinsic T cell determinant was pursued.
Hunt
J D
JD
Department of Microbiology, University of Melbourne, Australia.
Brown
L E
LE
Wood
P R
PR
Stewart
D J
DJ
Jackson
D C
DC
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D001426:Bacterial Proteins / Q000276:immunology; D002455:Cell Division; D010861:Fimbriae, Bacterial / Q000276:immunology; D005535:Foot Rot / Q000276:immunology / Q000517:prevention & control; D006089:Gram-Negative Anaerobic Bacteria / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000502:physiology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
D001426:Bacterial Proteins; D014614:Vaccines, Synthetic