title: Potential for diagnosis of intestinal nematode infections through antibody detection in saliva.
abstract: The present study was the first to investigate the potential of saliva in community diagnosis of the major human intestinal nematode infections, Trichuris trichiura, Ascaris lumbricoides, and the hookworms. Immunoglobulin G (IgG) antibodies specific to parasite antigens were quantified in saliva samples of 187 individuals (all ages) from a St Lucian community, and 120 school-aged children from Tanga region, Tanzania, and relationships with current infection status (determined by numbers of parasite eggs in stool) were examined. For T. trichiura infection, the age relationships of parasite-specific salivary IgG antibodies mirrored those of infection intensity at the community level. Within both areas, children with current T. trichiura infection exhibited significantly higher anti-T. trichiura salivary IgG responses than uninfected children. Similar trends were apparent for A. lumbricoides and hookworm infections, though not to a level of statistical significance. Comparison of mean T. trichiura infection levels and antibody responses in age-matched children from St Lucia and Tanzania suggested that measurement of parasite-specific salivary IgG may have potential as a marker of transmission intensity at the community level.
authors.affiliation: Department of Zoology, University of Oxford, UK.
authors.forename: C S
authors.initials: CS
authors.lastname: Needham
authors.forename: J E
authors.initials: JE
authors.lastname: Lillywhite
authors.forename: N M
authors.initials: NM
authors.lastname: Beasley
authors.forename: J M
authors.initials: JM
authors.lastname: Didier
authors.forename: C M
authors.initials: CM
authors.lastname: Kihamia
authors.forename: D A
authors.initials: DA
authors.lastname: Bundy
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000909:Antibodies, Helminth / Q000032:analysis*; D001196:Ascariasis / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006725:Hookworm Infections / Q000175:diagnosis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis*; D008875:Middle Aged; D009349:Nematode Infections / Q000175:diagnosis*; D010270:Parasite Egg Count; D012463:Saliva / Q000276:immunology*; D014257:Trichuriasis / Q000175:diagnosis
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G