High-performance liquid chromatographic analysis of ascorbyl-2-phosphate in fish tissues.
L-Ascorbic-2-phosphate magnesium salt (APM) in fish tissues was determined by high-performance liquid chromatography. APM extracted with 5% metaphosphoric acid was separated by a LiChrospher 100 RP18 column within 20 min. The detection limit was 0.1 microgram/g tissue.
Sakai
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Applied Biochemistry Division, Faculty of Agriculture, Miyazaki University, Japan.
Murata
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Ito
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D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002347:Carps; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D008099:Liver / Q000737:chemistry
C011669:ascorbate-2-phosphate; D001205:Ascorbic Acid