Characterization of p92, karyopherin beta, co-purified with N-acetylglucosamine-bearing nucleoporins from rat liver nuclear envelopes.
A 92k protein (p92) was purified from the wheat germ agglutinin-Sepharose (WGA-Sepharose) bound fraction of a rat liver nuclear envelope salt-extract by DEAE-5PW and hydroxyapatite HPLCs. Partial amino acid sequence analysis of p92 revealed that it is karyopherin beta, which was found recently in the cytosolic fraction. It was shown using anti-p92 antiserum that the protein is present in the nuclear envelope and cytosolic fractions, in almost the same amounts, but not in other subcellular fractions of rat liver. p92 bound to N-acetylglucosamine bearing nucleoporins (GNPs) on WGA-Sepharose, but not directly to WGA. The amount of p92 found in the rat liver nuclear envelope fraction corresponded to about 10% of the nuclear pore complex in mass, and to as much as 140 mol of p92 per mol of nuclear pore complex. Hydrodynamic analysis of the purified p92 suggested that the molecule is present as a monomer and that it is a rod-shaped molecule. The interaction of p92 and GNPs seemed to be hydrophobic and ionic. Based on these results, the participation of nuclear envelope p92 in protein nuclear transport is discussed.
Makifuchi
S
S
Department of Biochemistry and Biology, Faculty of Science, Niigata University.
Takeuchi
M
M
Kawahire
S
S
Odani
S
S
Ichimura
T
T
Omata
S
S
Horigome
T
T
D000117:Acetylglucosamine / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003600:Cytosol / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000737:chemistry*; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D028961:beta Karyopherins
D009687:Nuclear Proteins; D028961:beta Karyopherins; D000117:Acetylglucosamine