flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.
A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing plasmid, corrected the filamentous phenotype common to both sinR mutants; autolysin synthesis was restored partially and completely in the flaD1 and flaD (sinR) null strains, respectively. Flagellin synthesis and motility were not restored at all in either strain.
Rashid
M H
MH
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Sekiguchi
J
J
D001412:Bacillus subtilis / Q000166:cytology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005408:Flagellin / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000096:biosynthesis; D012808:Sigma Factor / Q000378:metabolism*
D001426:Bacterial Proteins; D012808:Sigma Factor; D005408:Flagellin; C076733:FlaD protein, Bacteria; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D009238:N-Acetylmuramoyl-L-alanine Amidase