A common-lines based method for determining orientations for N > 3 particle projections simultaneously.
A method is proposed for determining the directions of projections. An arbitrary number of projections of unknown three-dimensional structure are simultaneously used as input. The method is based on common lines and uses a new discrepancy measure accounting for the uneven distribution of common lines in angular space. An application to the 70S Escherichia coli ribosome data obtained from an energy-filtering electron microscope is described.
Penczek
P A
PA
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509, USA.
Zhu
J
J
Frank
J
J
D000465:Algorithms; D004926:Escherichia coli / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008433:Mathematics; D008854:Microscopy, Electron / Q000379:methods*; D012270:Ribosomes / Q000648:ultrastructure*