[Effective binding of alpha-bungarotoxin with the solubilized alpha-subunit of Torpedo californica acetylcholine receptor].
alpha-Subunit of the Torpedo californica nicotinic acetylcholine receptor was isolated by preparative SDS-PAGE followed by reversed-phase HPLC on a C4 column in an acetonitrile-isopropanol gradient in water. After removal of the organic solvents and solubilization in beta-octylglucoside, the purified alpha-subunit binds alpha-bungarotoxin with high affinity (Kd 28 nM).
Utkin
Iu N
IuN
Mund
M
M
Hucho
F
F
Tsetlin
V I
VI
D000818:Animals; D002038:Bungarotoxins / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility; D014101:Torpedo
D002038:Bungarotoxins; D011978:Receptors, Nicotinic