Identification of membrane-bound c-type cytochromes in an acidophilic ferrous ion oxidizing bacterium Thiobacillus ferrooxidans.
Three membrane-bound acid-stable cytochromes c with molecular masses of 46, 30 and 21 kDa were characterized from a new Thiobacillus ferrooxidans strain. They were solubilized with high concentrations of dodecylmaltoside at pH 8. The 30 kDa cytochrome c was purified to a homogeneous state as established by SDS-PAGE analysis. It showed an absorption peak at 410 nm in the oxidized form and at 418, 523 and 552 nm in the reduced form. The 46 kDa cytochrome c co-purified with a non-heme protein of 36 kDa. The amino acid composition and the N-terminal amino acid sequence of the 46 kDa cytochrome c were determined and compared with those of the soluble 14 kDa and the membrane-bound 21, 22.3 and 68 kDa cytochromes c isolated from two different strains. The results clearly show that this cytochrome is distinct from both the 22.3, 21 and 14 kDa cytochrome species, and exhibits some similarities with the 68 kDa cytochrome c as regards its amino acid composition.
Elbehti
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A
Laboratoire de Bioenergetique et Ingenierie des Proteines, Marseille, France.
Lemesle-Meunier
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D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003574:Cytochrome c Group / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008566:Membranes / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012995:Solubility; D013855:Thiobacillus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D000596:Amino Acids; D003574:Cytochrome c Group; D007501:Iron