Easy enzyme-linked immunosorbent assay for spectinomycin in chicken plasma.
An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for spectinomycin in chicken plasma was developed. Preparation of a spectinomycin-bovine serum albumin conjugate in which the hapten is linked to the carrier protein through the C-4 position is described. Antibodies raised against antigens in rabbits had excellent specificity for spectinomycin, exhibiting a cross-reactivity of 44.0% with dihydrospectinomycin and 13.8% with tetrahydrospectinomycin. No cross-reactivity was observed with other antibiotics. The detection limit of the CI-ELISA was 2 ng/mL (equivalent into 40 ng/mL undiluted chicken plasma) spectinomycin. Known amounts (0.1-100 micrograms/mL) of spectinomycin were added to chicken plasma and then analyzed. Average recoveries were 97-110%. This procedure may be used without prior extraction of samples.
Tanaka
T
T
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Kamiyoga, Tokyo, Japan.
Ikebuchi
H
H
Sawada
J I
JI
Okada
M
M
Kido
Y
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000276:immunology; D001667:Binding, Competitive; D002417:Cattle; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006241:Haptens / Q000737:chemistry; D010091:Oximes / Q000737:chemistry; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D000198:Spectinomycin / Q000097:blood* / Q000276:immunology; D013050:Spectrometry, Fluorescence; D014614:Vaccines, Synthetic / Q000737:chemistry
D000900:Anti-Bacterial Agents; D006241:Haptens; D010091:Oximes; D014614:Vaccines, Synthetic; D012710:Serum Albumin, Bovine; D000198:Spectinomycin