Specificities of five kinds of antisera produced against crude drugs, pinella tuber, hoelen, glycyrrhizae radix, trichosanthes root and panax ginseng.
Five kinds of rabbit antisera produced against five kinds of crude drugs, Pinella Tuber, Glycyrrhizae Radix (GR), Trichosanthes Root, Hoelen, and Panax Ginseng, and five kinds of extracts of the same crude drugs were prepared. Specificity of each antiserum was demonstrated by two immunological methods for analyses, the selected antibody enzyme immunoassay (SAEIA) and western blotting, and five crude drug extracts as specimens. Each crude drug extract contained characteristic antigen specific to the corresponding antiserum. Characteristic antigens were suggested as protein components. Characteristic antigen of the crude drug GR was separated from GR extract by three chromatographic procedures and a protein component was separated. The separated protein was successfully applied to develop a SAEIA method applicable for specific assays of both the separated protein and GR extract.
Kitagawa
T
T
Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
Bai
G
G
Fujiwara
K
K
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D005894:Panax / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits / Q000276:immunology
D004365:Drugs, Chinese Herbal; D010940:Plant Proteins