HTLV Tax gene expression in patients with lymphoproliferative disorders.
OBJECTIVE
To study the expression of the human T lymphotropic virus (HTLV) Tax gene in peripheral blood mononuclear cells.


METHODS
Blood was collected from 72 patients with lymphoproliferative disorders. Serum from all patients was assayed for antibodies directed against HTLV-I structural proteins by ELISA and western blotting. RNA was purified from fresh blood cells and amplified by reverse transcription polymerase chain reaction (RT-PCR). After Southern blotting, the PCR products were hybridised with a 32P end-labelled probe specific for the Tax gene.


RESULTS
All samples were seronegative. A specific band for the Tax gene was found in five samples. Each of the patients positive for Tax gene expression had a different type of lymphoproliferative disorder.


CONCLUSIONS
Infection by HTLV-I cannot be assessed solely by immunological assays, particularly when only disrupted virions are used. Sensitive molecular biology assays are essential for detecting viral gene expression in fresh blood cells.
Cardoso
E A
EA
Laboratório de Biologia Molecular (CIPM), Instituto Portugués de Oncologia, Lisboa, Portugal.
Miranda
N
N
Gameiro
P
P
Frade
M J
MJ
Figueiredo
M
M
Parreira
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood; D016355:Genes, pX / Q000502:physiology*; D015751:Genes, pol / Q000502:physiology*; D015490:HTLV-I Infections / Q000821:virology*; D006689:Hodgkin Disease / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000821:virology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
D000914:Antibodies, Viral