Imipramine-induced increase in 5-HT2C receptor mRNA level in the rat brain.
Repeated oral administration of 20 mg/kg imipramine elevated the level of 5-HT2C mRNA in the rat brain. Hybridization signals in nearly all regions stained by digoxigenin-labeled antisense cRNA probe, such as the hippocampus, choroid plexus, habenular nucleus, and dorsomedial hypothalamic nucleus, were more intense following imipramine treatment. These results suggest that long-term treatment with imipramine stimulates 5-HT2C receptor gene expression.
Tohda
M
M
Division of Pharmacology, Research Institute for Wakan-yaku, Toyama Medical and Pharmaceutical University, Japan.
Watanabe
H
H
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects*; D004076:Digoxigenin; D015870:Gene Expression / Q000187:drug effects; D007099:Imipramine / Q000494:pharmacology*; D017403:In Situ Hybridization; D008297:Male; D016376:Oligonucleotides, Antisense; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000187:drug effects
D000929:Antidepressive Agents, Tricyclic; D016376:Oligonucleotides, Antisense; D015347:RNA Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D004076:Digoxigenin; D007099:Imipramine