Characterization of IgG Fc receptors on CD34 antigen-expressing cell lines (KG-1 and KG-1a).
Although Fc gamma receptors (Fc gamma R) on mature blood cells have been extensively studied, there are only limited data on Fc gamma R expression in the early haematopoietic progenitor cells. In this study, we used the stem cell antigen (CD34)-expressing cell line (KG-1) and its less differentiated subline (KG-1a) as a model for the study of Fc gamma R in the early haematopoietic progenitors. Flow cytometry and immunoprecipitation studies on KG-1 and KG-1a cells with anti-Fc gamma R mAb showed that Fc gamma RII is the only Fc gamma R expressed on the cell surface. Analysis of the steady-state levels of Fc gamma R mRNA in KG-1 and KG-1a cells using a quantitative in situ hybridization assay revealed the presence of only Fc gamma RII mRNA. On further analysis Fc gamma RIIA mRNA but no Fc gamma RIIB or Fc gamma RIIC transcripts were found in these cells; Fc gamma RIIA transcripts with and without the transmembrane exon were present in approximately equal amounts. These findings are surprisingly similar to those observed previously with Fc gamma R in platelets and megakaryocytic cells but different from those found with Fc gamma R in cells of other lineages. These data suggest that the Fc gamma R transcript distribution pattern observed in the early haematopoietic progenitors (KG-1 cells) is retained in later stages of haematopoietic differentiation only in cells of megakaryocytic lineage.
Wu
Z
Z
Centre for Thrombosis and Vascular Research, University of New South Wales, Kensington, Australia.
Markovic
B
B
Chesterman
C N
CN
Chong
B H
BH
D018952:Antigens, CD34 / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D005434:Flow Cytometry; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011961:Receptors, Fc / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D018952:Antigens, CD34; D008565:Membrane Proteins; D012333:RNA, Messenger; D011961:Receptors, Fc