Outer membrane proteins and serosubtyping with outer membrane vesicles from clinical isolates of Neisseria meningitidis.
The currently practiced protocol for routine serosubtyping of Neisseria meningitidis relies on reactivity of whole cells to monoclonal antibodies against the class 1 outer membrane protein (OMP) in ELISAs or dot-blots. This procedure, however, failed to yield serosubtyping information in 28% (48/174) of clinical isolates (1993-1994) in the province of Québec, Canada. These 48 strains were characterized by OMP profiles and ELISAs with outer membrane vesicles (OMVs). Forty out of the 48 strains expressed class 1 OMP, indicating that the inability to assign a serosubtype was not owing to the absence of the class 1 OMP. Of these, 15 (38%) were serosubtypable in ELISAs with outer membrane vesicles. Thus, 81% (141/174) of all meningococcal strains were serosubtypable with ELISAs using whole-cells or OMVs. Because the routinely used procedure for serosubtyping of meningococci is limited in providing serosubtype information, alternate procedures are proposed to obtain comprehensive information for epidemiological identification of this bacterium.
Arhin
F F
FF
Meningococcal Research Laboratory, Department of Pediatrics, McGill University, Montréal, Québec, Canada.
Moreau
F
F
Coulton
J W
JW
Mills
E L
EL
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005798:Genes, Bacterial / Q000235:genetics; D009345:Neisseria meningitidis / Q000145:classification*; D018272:Porins / Q000235:genetics; D011792:Quebec; D012703:Serotyping / Q000379:methods*
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C057699:porin protein, Neisseria