title: Strong interaction between caldesmon and calponin.
abstract: Caldesmon was labeled at either Cys-153 in the NH2-terminal domain or Cys-580 in the COOH-terminal domain with a 6-acryloyl-2-dimethylaminonaphthalene (acrylodan) fluorescence probe. The addition of smooth muscle calponin to Cys-580-labeled caldesmon resulted in an 18% drop in fluorescence intensity, which titrated with a stoichiometry of 0.9 and a binding constant of 9.5 x 10(7) M-1. For Cys-153-labeled caldesmon, there was no change in fluorescence upon adding calponin. These findings indicate strong binding between calponin and the COOH-domain of caldesmon. The association was sensitive to ionic strength, suggesting that ionic interactions between calponin, a basic protein, and caldesmon, an acidic protein, contribute to the stabilization of the protein complex. That non-muscle acidic calponin interacts with caldesmon with a much reduced association constant of 3.5 x 10(6) M-1 supports such a model. The binding between acidic calponin and caldesmon is strengthened to 1.8 x 10(7) M-1 in the presence of Ca2+, which might bind to acidic residues of the calponin and partially neutralize its negative charge. The strong, specific binding between calponin and caldesmon suggests that this interaction occurs within smooth muscle cells and possibly plays a role in the regulation of contraction.
authors.affiliation: Boston Biomedical Research Institute, Boston, Massachusetts 02114, USA. graceffa@bbri.harvard.edu
authors.forename: P
authors.initials: P
authors.lastname: Graceffa
authors.forename: L P
authors.initials: LP
authors.lastname: Adam
authors.forename: K G
authors.initials: KG
authors.lastname: Morgan
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D002645:Chickens; D008840:Microfilament Proteins; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000502:physiology; D011485:Protein Binding; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013552:Swine; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D008840:Microfilament Proteins; D000096985:Calponins