title: Rapid method for detection of Coxiella burnetii antibodies using high-density particle agglutination.
abstract: A high-density particle agglutination test, using erythrocyte-sensitizing substance from phase II Coxiella burnetii adsorbed to high-density composite particles, was developed for rapid serodiagnosis of Q fever. The test was compared with the microimmunofluorescence test for sensitivity and specificity by using 3,036 human serum samples collected in Gifu Prefecture, Japan. An excellent agreement was found between the two tests for the acute-phase group and paired serum samples, but some discordant results were observed in the single-sample group. The sensitivity and specificity of the high-density particle agglutination test were both 100% in the former group and 81.6 and 99.9%, respectively, in the latter group. The test is a very promising tool for routine serodiagnosis of Q fever because of its simplicity, sensitivity, and specificity.
authors.affiliation: Department of Veterinary Microbiology, Gifu University, Japan.
authors.forename: S V
authors.initials: SV
authors.lastname: Nguyen
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: To
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.forename: A
authors.initials: A
authors.lastname: Noma
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000372:Agglutination Tests / Q000379:methods* / Q000706:statistics & numerical data; D000907:Antibodies, Bacterial / Q000097:blood*; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D016997:Coxiella burnetii / Q000276:immunology*; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D011778:Q Fever / Q000175:diagnosis* / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D000907:Antibodies, Bacterial