SNAP-25 is present on chromaffin granules and acts as a SNAP receptor.
SNAP-25 is located on the plasma membrane and essential for exocytosis of neurotransmitters. It was suggested that SNAP-25 and syntaxin 1 via the interaction with VAMP-2 located on synaptic vesicles mediate the docking of the vesicles with the plasma membrane. In the present study, by means of biochemical and morphological analyses, we showed that SNAP-25 is present on chromaffin granules as well as on the plasma membrane. Reconstitution and immunoprecipitation analyses revealed that SNAP-25 on chromaffin granules has essentially the same properties as does SNAP-25 on the plasma membrane.
Tagaya
M
M
School of Life Science, Tokyo University of Pharmacy and Life Science, Japan.
Genma
T
T
Yamamoto
A
A
Kozaki
S
S
Mizushima
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002458:Cell Fractionation; D002837:Chromaffin Granules / Q000737:chemistry* / Q000378:metabolism; D005089:Exocytosis / Q000502:physiology; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D050683:R-SNARE Proteins; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins