Transcriptionally active chromosomes (TACs) of barley chloroplasts contain the alpha-subunit of plastome-encoded RNA polymerase.
Transcriptionally active chromosomes (TACs) were isolated from mature chloroplasts of barley, from proplastids enriched in basal segments of barley primary foliage leaves, and from ribosome-deficient plastids of heat-bleached barley leaves. Immunological analysis with a specific antibody raised against the plastid rpoA gene product revealed that chloroplasts contain an immunoreactive protein of 38 kDa in the TAC fraction which appears to be identical to the alpha-subunit contained in the soluble RNA polymerase (sRNAP) fraction of the same chloroplasts. However, only traces of immunoreactive protein were detected in a TAC preparation derived from "proplastids". A positive correlation could be demonstrated between transcriptional activity and the amount of immunoreactive 38-kDa protein by analyzing different TAC fractions eluting at different times during gel filtration of a standard TAC preparation as well as in TAC preparations obtained under various detergent conditions.
Suck
R
R
Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, D-50931 Köln, Germany.
Zeltz
P
P
Falk
J
J
Acker
A
A
Kössel
H
H
Krupinska
K
K
D002736:Chloroplasts / Q000201:enzymology / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D007124:Immunoenzyme Techniques; D018087:Plastids / Q000235:genetics; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics
D012321:DNA-Directed RNA Polymerases