Heterogeneity of hypotonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueous polymer two-phase systems.
Carrier rat erythrocytes loaded with exogenous substances ([125I] carbonic anhydrase) by hypotonic-isotonic dialysis become heterogeneous cell populations that can be fractionated using the counter-current distribution (CCD) technique. Two well-defined low- and high-partition ratio, G, subpopulations are obtained in charge-sensitive dextran-polyethylene glycol two-phase systems. The low-G subpopulation, which contains the most fragile and surface-altered cells, as deduced from their osmotic fragility curves and partition behaviour, respectively, presents a high amount of exogenous substance incorporated (134.6 cpm/10(6) cells). The high-G subpopulation, that contains cells similar to the control or isotonically dialyzed cells presents a lower amount of exogenous substance incorporated (69.8 cpm/10(6) cells). Cells in this high-G subpopulation seem to be fractionated, like the controls, according to ageing as suggested by the decline of the pyruvate kinase specific activity from the left- to the right-hand side of the CCD profile.
Pérez
M T
MT
Departmento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Alvarez
F J
FJ
García-Pérez
A I
AI
Lucas
L
L
Tejedor
M C
MC
Sancho
P
P
D000818:Animals; D001772:Blood Cell Count; D002256:Carbonic Anhydrases / Q000097:blood; D003377:Countercurrent Distribution; D003956:Dialysis; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D009996:Osmotic Fragility; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar
D007038:Hypotonic Solutions; D011108:Polymers; D002256:Carbonic Anhydrases