An apoptotic response to photodynamic therapy with endogenous protoporphyrin in vivo.
CDF1 mice bearing the colon-26 tumor were treated with aminolevulinic acid (200 mg kg-1) by tail-vein injection, with tumor sites irradiated 4 h later at 633 nm (75-120 J cm-2). 10 h after irradiation, samples of tumors were removed for histology studies and analysis of DNA fragmentation by static gel electrophoresis. The resulting patterns indicate an apoptotic response to photodynamic therapy with endogenously formed protoporphyrin.
Webber
J
J
Department of Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Luo
Y
Y
Crilly
R
R
Fromm
D
D
Kessel
D
D
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002330:Carmustine / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000628:therapy; D053938:DNA Fragmentation; D004317:Doxorubicin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D051379:Mice; D017239:Paclitaxel / Q000494:pharmacology; D010778:Photochemotherapy; D011524:Protoporphyrins / Q000627:therapeutic use*
D011524:Protoporphyrins; D005047:Etoposide; D004317:Doxorubicin; D017239:Paclitaxel; D002330:Carmustine