title: Disregulated expression of CD43 (leukosialin, sialophorin) in the B cell lineage leads to immunodeficiency.
abstract: Leukosialin (CD43 or sialophorin) is a cell surface sialoglycoprotein implicated in cell adhesion and proliferation whose tightly regulated expression in B lymphocytes is likely important for their normal development and/or function. To examine the physiologic role of mouse CD43 (mCD43) in vivo, we exploited transgenic (TG) mice whose developmental expression of mCD43 was extended during B cell differentiation so that mCD43 was now expressed on peripheral B cells. Despite having increased B cells, localization of lymphocytes in the TG spleens appeared normal by immunocytochemistry with anti-CD4, anti-CD8, and anti-B220 mAbs. However, the numbers of splenic germinal centers and the resting sera Ig levels were decreased in the TG mice compared with littermate controls. TG mice had decreased humoral responses to the T-dependent Ags keyhole limpet hemocyanin and OVA, as well as reduced Ag-specific B cell numbers. In contrast, in vitro LPS stimulation of purified TG or control B cells resulted in similar proliferation and IgM responses. Thus, the alteration of B cell mCD43 expression that resulted in profound immunodeficiency in vivo was not due to absolute defects in B cell development or Ab production. However, TG B cells had a decreased ability to homotypically aggregate and to present Ag to the T cell hybridoma B3Z. These data suggest that the immunodeficiency seen in vivo is due to the anti-adhesive forces of mCD43 preventing normal T-B cell interaction. This likely reflects a general property of mucins in regulating cell interactions.
authors.affiliation: Department of Microbiology and Immunology, and the Cancer Center, University of Rochester Medical Center, NY 14642, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Ostberg
authors.forename: L L
authors.initials: LL
authors.lastname: Dragone
authors.forename: T
authors.initials: T
authors.lastname: Driskell
authors.forename: J A
authors.initials: JA
authors.lastname: Moynihan
authors.forename: R
authors.initials: R
authors.lastname: Phipps
authors.forename: R K
authors.initials: RK
authors.lastname: Barth
authors.forename: J G
authors.initials: JG
authors.lastname: Frelinger
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation; D015870:Gene Expression; D006433:Hemocyanins / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism; D007153:Immunologic Deficiency Syndromes / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D051917:Leukosialin; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008954:Models, Biological; D010047:Ovalbumin / Q000276:immunology; D012795:Sialoglycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D015703:Antigens, CD; D007075:Immunoglobulin M; D007136:Immunoglobulins; D051917:Leukosialin; D008070:Lipopolysaccharides; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; D010047:Ovalbumin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin