title: Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway.
abstract: Cell cycle regulation is critical for maintenance of genome integrity. A prominent factor that guarantees genomic stability of cells is p53 (ref. 1). The P53 gene encodes a transcription factor that has a role as a tumour suppressor. Identification of p53-target genes should provide greater insight into the molecular mechanisms that mediate the tumour suppressor activities of p53. The rodent Pc3/Tis21 gene was initially described as an immediate early gene induced by tumour promoters and growth factors in PC12 and Swiss 3T3 cells. It is expressed in a variety of cell and tissue types and encodes a remarkably labile protein. Pc3/Tis21 has a strong sequence similarity to the human antiproliferative BTG1 gene cloned from a chromosomal translocation of a B-cell chronic lymphocytic leukaemia. This similarity led us to speculate that BTG1 and the putative human homologue of Pc3/Tis21 (named BTG2) were members of a new family of genes involved in growth control and/or differentiation. This hypothesis was recently strengthened by the identification of a new antiproliferative protein, named TOB, which shares sequence similarity with BTG1 and PC3/TIS21 (ref. 7). Here, we cloned and localized the human BTG2 gene. We show that BTG2 expression is induced through a p53-dependent mechanism and that BTG2 function may be relevant to cell cycle control and cellular response to DNA damage.
authors.affiliation: Unité INSERM U453, Affiliée au CNRS, Centre Léon Bérard, Lyon, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Rouault
authors.forename: N
authors.initials: N
authors.lastname: Falette
authors.forename: F
authors.initials: F
authors.lastname: Guéhenneux
authors.forename: C
authors.initials: C
authors.lastname: Guillot
authors.forename: R
authors.initials: R
authors.lastname: Rimokh
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Berthet
authors.forename: C
authors.initials: C
authors.lastname: Moyret-Lalle
authors.forename: P
authors.initials: P
authors.lastname: Savatier
authors.forename: B
authors.initials: B
authors.lastname: Pain
authors.forename: P
authors.initials: P
authors.lastname: Shaw
authors.forename: R
authors.initials: R
authors.lastname: Berger
authors.forename: J
authors.initials: J
authors.lastname: Samarut
authors.forename: J P
authors.initials: JP
authors.lastname: Magaud
authors.forename: M
authors.initials: M
authors.lastname: Ozturk
authors.forename: C
authors.initials: C
authors.lastname: Samarut
authors.forename: A
authors.initials: A
authors.lastname: Puisieux
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004249:DNA Damage; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D017874:Immediate-Early Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D025521:Tumor Suppressor Proteins
chemical_list: C489552:Btg1 protein, mouse; C489435:Btg2 protein, mouse; D017874:Immediate-Early Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C070114:BTG2 protein, human; C074086:BTG1 protein, human