Peptides bound endogenously by HLA-Cw*0304 expressed in LCL 721.221 cells include a peptide derived from HLA-E.
The peptide-binding specificity of HLA-Cw*0304 was determined. Sequence analysis of endogenously-bound peptides isolated from Cw*0304 expressed by LCL 721.221 (221 for short) cells transfected with Cw*0304 cDNA revealed this class I allotype preferentially binds peptides possessing alanine at position 2 and leucine or methionine at the C-terminus. One peptide isolated from Cw*0304 expressed by 221 cells has sequence identity to residues 116-126 of HLA-E. Expression of HLA-E by 221 cells was confirmed by isolation of mRNA transcripts for HLA-E*0101 and detection of beta 2-microglobulin (beta 2-m)-associated HLA-E protein.
Tzeng
C M
CM
Department of Structural Biology, Stanford University, California, USA.
Adams
E J
EJ
Gumperz
J E
JE
Percival
L
L
Wells
R S
RS
Parham
P
P
Barber
L D
LD
D002461:Cell Line, Transformed; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D015236:HLA-C Antigens / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000276:immunology*; D012333:RNA, Messenger; D000097625:HLA-E Antigens
D006680:HLA Antigens; D015236:HLA-C Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D012333:RNA, Messenger; D000097625:HLA-E Antigens; C062341:HLA-F antigens