Plasma activation of neutrophil CD18 after skeletal muscle ischemia: a potential mechanism for late systemic injury.
Reperfusion of acutely ischemic skeletal muscle is associated with neutrophil activation, which may augment local injury or cause damage to distant organs. Polymorphonuclear neutrophil glycoprotein CD18 plays a role in this injury, since its blockade substantially reduces damage; however, its mechanisms of control during reperfusion are poorly understood. The purpose of this study was to investigate the importance of circulating plasma factors to CD18-dependent neutrophil function during reperfusion and to relate these to quantitative expression of CD18. Eight rabbits were subjected to hindlimb ischemia for 5 h, followed by 48 h of reperfusion. Plasma collected at seven intervals was incubated with unstimulated neutrophils from uninjured rabbits. CD18-specific neutrophil activation was evaluated by quantifying adherence to protein-coated polystyrene and by measuring oxidant production, detected by chemiluminescence after exposure to complement-opsonized zymosan. CD18 was quantified cytofluorometrically. Plasma collected at end ischemia and during early reperfusion affected no significant alterations of adhesion, oxidant production, or CD18. Late reperfusion plasma (between 8 and 48 h) significantly increased adherence and oxidant production (to 4.11 +/- 0.61 and 2.60 +/- 0.32 times the values of preischemic plasma, P < 0.006). Peak adherence, oxidant production, and CD18 expression were evoked synchronously by 24 h plasma. CD18 expression increased only at 24 h and did not increase proportional to increases in adherence and oxidant production. Control plasma (nonischemic, n = 5) elicited no significant differences of any inflammatory measure during sham ischemia or reperfusion. These results indicate that endogenous mediators may evoke a progressive systemic inflammatory response after ischemia by stimulating CD18-dependent neutrophil function in a delayed but prolonged manner.
Petrasek
P F
PF
R. Fraser Elliot Vascular Surgery Research Laboratory, Toronto Hospital, University of Toronto, Ontario, Canada.
Lindsay
T F
TF
Romaschin
A D
AD
Walker
P M
PM
D000818:Animals; D018821:CD18 Antigens / Q000276:immunology*; D002448:Cell Adhesion; D005260:Female; D007511:Ischemia / Q000097:blood / Q000276:immunology*; D018482:Muscle, Skeletal / Q000098:blood supply*; D018375:Neutrophil Activation / Q000502:physiology*; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology; D010949:Plasma / Q000502:physiology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000276:immunology / Q000503:physiopathology
D018821:CD18 Antigens; D017382:Reactive Oxygen Species