Seroreactivity of Salmonella-infected cattle herds against a fimbrial antigen in comparison with lipopolysaccharide antigens.
The IgG seroreaction of Salmonella-infected cattle herds against a fimbrial antigen (SEF14) was compared with that against lipopolysaccharide (LPS) antigens. Sera from 23 dairy herds (n = 205) from an island with no occurrence of salmonellosis, four herds (n = 303) with recent outbreaks of S. dublin and four herds (n = 168) with recent outbreaks of S. typhimurium, were tested in a SEF14-ELISA, S. dublin LPS (0:1, 9, 12) ELISA and S. typhimurium LPS (0:1, 4, 5, 12) ELISA. At a cut-off OD of 0.5, only one of the animals tested from the salmonellosis-free island showed significant seroreaction against the SEF14 antigen, which was confirmed in a Western-blot analysis. Three out of the four S. dublin-infected herds had several seroreactors in the SEF14-ELISA, whereas all the four herds were positive in the 0:1, 9, 12-ELISA. All but two samples (both from the same herd) in the four S. typhimurium-infected herds, positive in the 0:1, 4, 5, 12-ELISA, had OD values below 0.5 in the SEF14-ELISA. The results indicate that cattle can produce detectable specific antibodies against fimbrial antigens which may be used for screening of S. dublin-infected herds, particularly in areas with low prevalence of salmonellosis, increasing the predictive value of serology.
Hoorfar
J
J
Danish Veterinary Laboratory, Copenhagen, Denmark, UK.
Lind
P
P
Bell
M M
MM
Thorns
C J
CJ
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000276:immunology* / Q000382:microbiology; D002418:Cattle Diseases / Q000276:immunology* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides