Presence of neuropeptide Y Y1 receptor mediating vasoconstriction in human cerebral arteries.
The neuropeptide Y-induced vasoconstriction of human cerebral arteries is mediated by the neuropeptide Y Y1 receptor. We conclude this on the basis of our results from: (1) in vitro studies on neuropeptide Y agonists. Neuropeptide Y and pro34NPY caused potent and long-lasting contractions of human cerebral arteries, while NPY 13-36 had no contractile effect at all on the vessels tested; (2) in vitro studies using the selective Y1 receptor antagonist BIBP3226 which in increasing concentrations (10(-9)-10(-6)M) caused a parallel shift to the right of the neuropeptide Y concentration-response curve without change of the maximum contractile response (pA2 value 8.38 +/- 0.10); and (3) with reverse transcriptase-polymerase chain reaction (RT-PCR) we detected specific mRNA for a neuropeptide Y Y1 receptor in human pial and human middle cerebral arteries using three forward primers and one reverse primer.
Nilsson
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T
Division of Experimental Vascular Research, Department of Internal Medicine, Lund University Hospital, Sweden.
Cantera
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Edvinsson
L
L
D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D002536:Cerebral Arteries / Q000737:chemistry* / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000819:agonists* / Q000031:analogs & derivatives; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors / Q000235:genetics*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
C092926:BIBP 3226; D017931:DNA Primers; D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; D001120:Arginine