Detection of cyanobacterial toxins (microcystins) in cell extracts by micellar electrokinetic chromatography.
A micellar electrokinetic chromatography (MEKC) method with UV detection is described for the rapid and efficient separation of three microcystins: microcystin-YR (MCYST-YR), microcystin-LR (MCYST-LR) and microcystin-RR (MCYST-RR). A detection limit of 7.5 pg for each toxin was achieved. The UV intensities of these toxins measured at 200 nm showed good linearity in the range 7.5-150 pg. The production of MCYST-LR in three cultured strains of cyanobacteria, namely Microcystis aeruginosa strain IP7806, Microcystis aeruginosa strain IP7813 and Oscillatoria agardhii strain IP7805, was evaluated.
Bouaïcha
N
N
CEMATMA, Laboratoire de Chimie Analytique, ESPCI, Paris, France.
Rivasseau
C
C
Hennion
M C
MC
Sandra
P
P
D001427:Bacterial Toxins / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008387:Marine Toxins; D008823:Micelles; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D014466:Ultraviolet Rays
D001427:Bacterial Toxins; D008387:Marine Toxins; D008823:Micelles; D052998:Microcystins; D010456:Peptides, Cyclic; C065685:microcystin YR; C063855:microcystin RR; C057862:cyanoginosin LR