The role of vascular endothelial growth factor in a murine chronic granulomatous tissue air pouch model of angiogenesis.
Chronic granulomatous inflammation may be considered an angiogenic-dependent process. Recently it has been demonstrated that vascular endothelial growth factor (VEGF) or vascular permeability factor is essential for tumour angiogenesis. Its role in inflammation-mediated angiogenesis has yet to be determined. In this study, the murine chronic granulomatous air pouch model was used to investigate the role of VEGF in angiogenesis. Animals were treated twice weekly with 10 micrograms per animal of neutralizing antibody to rh VEGF and the vascularity and granuloma dry weight were assessed after 7 days. This resulted in significant suppression of both angiogenesis and granuloma dry weight. Western blot analysis demonstrated the presence of VEGF; the levels of protein paralleled the angiogenic response. These results demonstrate for the first time that VEGF may be an important regulator of angiogenesis in inflammation.
Appleton
I
I
Department of Experimental Pathology, St Bartholomew's Hospital Medical College, Charterhouse Square, London, U.K.
Brown
N J
NJ
Willis
D
D
Colville-Nash
P R
PR
Alam
C
C
Brown
J R
JR
Willoughby
D A
DA
D000388:Air; D000818:Animals; D015153:Blotting, Western; D002908:Chronic Disease; D004195:Disease Models, Animal; D016228:Endothelial Growth Factors / Q000276:immunology / Q000502:physiology*; D005260:Female; D006099:Granuloma / Q000150:complications / Q000209:etiology / Q000378:metabolism*; D008222:Lymphokines / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors