title: Colicins B and Ia as novel counterselective agents in interspecies conjugal DNA transfers from colicin-sensitive Escherichia coli donors to other gram-negative recipient species.
abstract: Colicins are powerful counterselective agents in conjugal DNA transfers using colicin-sensitive Escherichia coli donor strains and colicin-resistant recipient species. We have constructed a high-level constitutive expression system for the bacteriocin and immunity genes of channel-forming colicins B and Ia to optimize production of these colicins as molecular biology reagents.
authors.affiliation: Department of Microbiology and Immunology, East Carolina University School of Medicine, Biotechnology, Greenville, NC 27858-4354, USA. brickman@brody.med.ecu.edu
authors.forename: T J
authors.initials: TJ
authors.lastname: Brickman
authors.forename: S K
authors.initials: SK
authors.lastname: Armstrong
mesh_terms: D003086:Bacteriocin Plasmids / Q000235:genetics; D003087:Colicins / Q000235:genetics* / Q000494:pharmacology; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D013045:Species Specificity
chemical_list: D003087:Colicins; D004269:DNA, Bacterial