High-performance liquid chromatographic analysis for the determination of miconazole in human plasma using solid-phase extraction.
A high-performance liquid chromatographic method for the determination of miconazole in human plasma is described. A solid-phase extraction was performed on an octadecyl (C18) cartridge. Miconazole was eluted with methanol, separated on a reversed-phase column and was measured by ultraviolet detection at 230 nm. The absolute extraction recovery from plasma samples was 85%. The limit of detection was established as 5 ng/ml. The coefficient of variation of the determination of plasma levels by this method over the standard curve concentration range was less than 10%, except with the concentration of 10 ng/ml. The plasma levels of miconazole in twelve healthy volunteers given a 250-mg oral dose of two tablet forms were determined by this method.
Kobylińska
M
M
Department of Pharmacology, Pharmaceutical Research Institute, Warsaw, Poland.
Kobylińska
K
K
Sobik
B
B
D000328:Adult; D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008825:Miconazole / Q000097:blood*; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D008825:Miconazole