Tumor-specific accumulation of 125I-labeled mouse-human chimeric anti-CEA antibody in a xenografted human cancer model demonstrated by whole-body autoradiography and immunostaining.
Whole-body autoradiography (WBAR) was used to study the biodistribution of 125I-labeled mouse-human chimeric antibody (Ch F11-39) to carcinoembryonic antigen (CEA) in athymic nude mice bearing the CEA-producing MKN-45 human gastric carcinoma xenografts. Significantly high uptake of 125I-Ch F11-39 in the tumors obtained by tissue-counting technique was confirmed by WBAR of mice of 12, 24, 48, and 96 h postinjection of 125I-Ch F11-39. When compared with histochemical or immunohistochemical staining results of the tumor tissue sections, imaging profiles of 125I-Ch F11-39 obtained by WBARs were topographically correlated with histopathological findings of tissues and immunohistochemical localization of CEA in the tumor tissues, indicating that the accumulation of 125I-Ch F11-39 at the tumor site is based on its specificity for CEA. These results demonstrate that this chimeric antibody may serve as a potential useful diagnostic and/or therapeutic reagent for human CEA-producing cancers.
Haruno
M
M
First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Kuroki
M
M
Matsunaga
K
K
Takata
J
J
Karube
Y
Y
Senba
T
T
Murakami
M
M
Arakawa
F
F
Kuwahara
M
M
Ozaki
H
H
Matsuoka
Y
Y
Okazaki
M
M
Kanda
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics*; D001345:Autoradiography; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011877:Radionuclide Imaging; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000493:pharmacokinetics*; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014915:Whole-Body Counting
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007457:Iodine Radioisotopes; D011993:Recombinant Fusion Proteins