title: Cytokine production in hearts of Trypanosoma cruzi-infected CBA mice: Do cytokine patterns in chronic stage reflect the establishment of myocardial pathology?
abstract: The authors analysed cytokine production in hearts of Trypanosoma cruzi-infected CBA-J mice by in situ immunocytochemical staining. Cellular infiltrates were recorded in hearts from both acute and chronic stages, but were not apparent in control hearts. In the acute heart, CD8 cells predominated, with associated production of IL-4, IL-6 and TNF-alpha. Cytokine production was characterized by IL-4, IL-5, IL-6 and TNF-alpha in the chronic heart, and numbers of CD4 and CD8 cells were more equal. At this stage, calcified infarctions and associated fibrosis were apparent, mimicking chronic human Chagas' heart pathology. The authors consider the CBA mouse an appropriate model of chronic T. cruzi infection, and suggest that local cytokine production reflects establishment of heart pathology.
authors.affiliation: Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Sunnemark
authors.forename: A K
authors.initials: AK
authors.lastname: Ulfgren
authors.forename: A
authors.initials: A
authors.lastname: Orn
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
mesh_terms: D000208:Acute Disease; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D002598:Chagas Cardiomyopathy / Q000378:metabolism* / Q000473:pathology; D002908:Chronic Disease; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004195:Disease Models, Animal; D004719:Endomyocardial Fibrosis / Q000209:etiology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats; D013154:Spleen / Q000276:immunology / Q000473:pathology; D014349:Trypanosoma cruzi; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D015848:Interleukin-5; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4