Stage-specific expression of mouse BST-1/BP-3 on the early B and T cell progenitors prior to gene rearrangement of antigen receptor.
Human bone marrow stromal cell antigen 1 (BST-1) was identified as a glycosylphosphatidyl-inositol-anchored ectoenzyme expressed on bone marrow stromal or synovial cell lines and having the ability to facilitate pre-B cell line growth. The analysis of the expression of mouse BST-1/BP-3 on the surface of lymphoid cells in the bone marrow and thymus revealed that it was very transiently expressed on both B and T cell progenitors undergoing gene rearrangement of the antigen receptor. Among CD45R+ CD43+ B cell progenitors in the bone marrow, BST-1 expression appeared on the CD24 (heat stable antigen)+, CD19+ or CD117 (c-kit)+ population. In the thymus, BST-1 was expressed on CD4-CD8-CD3- [triple negative (TN)] CD90 (Thy-1)+ cells. In TN thymocytes, the majority of CD25+ cells and CD44(10)/- cells expressed BST-1. In fetuses, BST-1+ cells appeared in the thymus and liver at day 14 and 16 of gestation respectively. The expression level of BST-1 by fetal thymus was maximal and > 60% of thymocytes were positive for BST-1 at day 15 or 16 and the proportion then gradually decreased during development. Among day 15 fetal thymocytes, BST-1 was negative on the CD44+ CD25- fraction, very slightly positive on the CD44+ CD25+ fraction, and strongly positive on the CD44(10)/- CD25+ and CD44-CD25- fractions. These results showed that murine BST-1 is a useful marker for lymphoid progenitor cells initiating gene rearrangement of their antigen receptors.
Ishihara
K
K
Department of Molecular Oncology, Osaka University Medical School, Japan.
Kobune
Y
Y
Okuyama
Y
Y
Itoh
M
M
Lee
B O
BO
Muraoka
O
O
Hirano
T
T
D036541:ADP-ribosyl Cyclase; D000818:Animals; D000918:Antibody Specificity; D015703:Antigens, CD; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D015153:Blotting, Western; D001853:Bone Marrow / Q000196:embryology; D001854:Bone Marrow Cells; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental; D015321:Gene Rearrangement; D005865:Gestational Age; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology
D015703:Antigens, CD; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2