Nitric oxide enhances 12-HETE versus LTB4 generation in pancreatic transplantation.
The role of nitric oxide in lipoxygenase metabolism after a process of ischemia-reperfusion in pancreas transplantation has been evaluated in this study. Sprague-Dawley rats were randomized into three groups, as follows: Group I--Control animals not surgically manipulated; Group II.--Pancreas transplantation, after 12 h of organ preservation; Group III.--Same as II but with administration of NG-nitro-L-arginine methyl esther (a nitric oxide synthase inhibitor) (10 mg/Kg) prior to organ revascularization. The results show post-transplantation increases in leukotriene B4 and 12-hydroxyeicosatraenoic acid levels in pancreatic tissue. Nitric oxide synthase inhibition reversed the increases in 12-hydroxyeicosatetraenoic acid, but was unable to modify leukotriene B4 increases suggesting the existence of a direct effect of nitric oxide on the 12-lipoxygenase metabolism in pancreas transplantation.
Hotter
G
G
Department of Medical Bioanalysis, CID, CSIC, Barcelona, Spain.
Closa
D
D
Pí
F
F
Fernández-Cruz
L
L
Gelpí
E
E
Roselló-Catafau
J
J
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000096:biosynthesis* / Q000235:genetics; D000208:Acute Disease; D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005609:Free Radicals; D005786:Gene Expression Regulation / Q000187:drug effects*; D007511:Ischemia / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D010179:Pancreas / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology; D016035:Pancreas Transplantation; D010195:Pancreatitis / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D004791:Enzyme Inhibitors; D005609:Free Radicals; D007975:Leukotriene B4; D009569:Nitric Oxide; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001092:Arachidonate 12-Lipoxygenase; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester