Chronic ethanol on mRNA levels of IP3R1, IP3 3-kinase and mGluR1 in mouse Purkinje neurons.
Chronic ethanol exposure is known to alter the polyphosphoinositide (poly-PI) signaling pathway, although the exact sight of regulation is not well understood. In this study, C57B1/6J mice were given a control or an ethanol (6 g kg-1 body wt) liquid diet by gastric intubation daily for 4 weeks. Examination of levels of mRNA for type 1 inositol 1,4,5-trisphosphate receptor (IP3R1), inositol 1,4,5-trisphosphate 3-kinase (IP3K) and metabotropic glutamate receptor 1 (mGluR1) in cerebellar Purkinje neurons by in situ hybridization revealed significant decreases in cellular levels of mRNA encoding both IP3R1 and mGluR1 in the ethanol-treated group compared with controls, but levels of IP3K mRNA were not altered. These results suggest that chronic ethanol consumption selectively alters cellular mRNA in the poly-PI signaling pathway. The decrease in mRNA levels of mGluR1 and IP3R1 may be an important underlying mechanism towards explaining the alcohol-induced degenerative changes in the cerebellar Purkinje neurons.
Simonyi
A
A
Department of Biochemistry, University of Missouri, Columbia 65212, USA.
Zhang
J P
JP
Sun
A Y
AY
Sun
G Y
GY
D000437:Alcoholism / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015220:Calcium Channels / Q000096:biosynthesis*; D002531:Cerebellum / Q000378:metabolism* / Q000473:pathology; D017403:In Situ Hybridization; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis*; D011689:Purkinje Cells / Q000378:metabolism* / Q000473:pathology; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis*; D012016:Reference Values; D014158:Transcription, Genetic
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015347:RNA Probes; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018094:Receptors, Metabotropic Glutamate; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor)