Modification of membrane fatty acid composition, eicosanoid production, and phospholipase A activity in Atlantic salmon (Salmo salar) gill and kidney by dietary lipid.
Atlantic salmon post-smolts were fed diets containing either fish oils (Fosol, FO and Marinol, MO) rich in long-chain n-3 polyunsaturated fatty acids (PUFA), or plant oils rich in 18:2n-6 (sunflower oil, SO) or 18:3n-3 (linseed oil, LO) for 12 wk. The major PUFA in individual phospholipids from gill and kidney were related to the dietary lipid intake. Levels of n-6 PUFA were highest while levels of n-3 PUFA were lowest in fish fed SO. Fish fed LO generally had lower levels of 20:4n-6 compared to the other treatments while fish fed SO generally had the highest levels of 20:4n-6. In all phospholipid classes except phosphatidylinositol (PI) 20:5n-3 was greatest in fish fed MO followed by FO, LO, and SO. In PI, 20:5n-3 was also highest in fish fed MO but those fed LO contained more 20:5n-3 than those fed FO. This resulted in the ratio of the eicosanoid precursors, 20:4n-6/20:5n-3, being significantly greater in fish fed SO, for all phospholipid classes, compared to fish fed the other three dietary oils. The activity of gill phospholipase A was greatest in fish fed FO and was lowest in fish fed SO. The concentration of PGF3 alpha was significantly increased in gill homogenates from fish fed MO compared to the other three treatments while PGF2 alpha was significantly increased in fish fed SO compared to those fed LO. The concentration of PGE3 was significantly reduced in kidney homogenates from fish fed SO compared to the other three treatments while PGE2 was significantly increased in fish fed SO compared to those fed either FO or LO.
Bell
J G
JG
Department of Biological and Molecular Science, University of Stirling, Scotland, United Kingdom.
Farndale
B M
BM
Dick
J R
JR
Sargent
J R
JR
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D015777:Eicosanoids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005395:Fish Oils / Q000008:administration & dosage / Q000737:chemistry; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D012474:Salmon / Q000378:metabolism*
D004042:Dietary Fats, Unsaturated; D015777:Eicosanoids; D005227:Fatty Acids; D005395:Fish Oils; D008563:Membrane Lipids; D010743:Phospholipids; D010938:Plant Oils; D011458:Prostaglandins E; D011460:Prostaglandins F; D010741:Phospholipases A