title: Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin.
abstract: The Escherichia coli heat-labile enterotoxin (LT) is a potent inducer of mucosal immune responses. In a previous study (L. DeHaan, W. R. Verweij, M. Holtrop, E. Agsteribbe, and J. Wilschut, Vaccine 14:620-626, 1996), we have shown that efficient induction of an LTB-specific mucosal immune response by LT requires the presence of the LTA chain, suggesting a possible role of the enzymatic activity of LTA in the induction of these responses. In the present study, we generated LT mutants with altered ADP-ribosylation activities and evaluated their immunogenicity upon intranasal administration to mice. The results demonstrate that the mucosal immunogenicity of LT is not dependent on its ADP-ribosylation activity.
authors.affiliation: Department of Physiological Chemistry, Groningen Institute for Drug Studies, University of Groningen, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: de Haan
authors.forename: W R
authors.initials: WR
authors.lastname: Verweij
authors.forename: I K
authors.initials: IK
authors.lastname: Feil
authors.forename: T H
authors.initials: TH
authors.lastname: Lijnema
authors.forename: W G
authors.initials: WG
authors.lastname: Hol
authors.forename: E
authors.initials: E
authors.lastname: Agsteribbe
authors.forename: J
authors.initials: J
authors.lastname: Wilschut
mesh_terms: D000244:Adenosine Diphosphate / Q000235:genetics / Q000276:immunology; D000818:Animals; D004768:Enterotoxins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D006358:Hot Temperature; D018928:Immunity, Mucosal; D051379:Mice; D009154:Mutation
chemical_list: D004768:Enterotoxins; D000244:Adenosine Diphosphate