title: Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species.
abstract: We examined aerobic, thermophilic, gram-negative bacteria that were isolated from hot springs in Portugal and were identified as Thermus strains and placed in phenetic groups on the basis of their phenotypic characteristics. We determined the composition of the peptidoglycan, identified the respiratory quinones, and determined the mean base composition of the DNA, and the levels of DNA-DNA homology were determined by both the filter hybridization and reassociation rate methods. Thermus aquaticus, Thermus brockianus, and Thermus filiformis were not detected in this collection of organisms, although three Thermus thermophilus strains were identified. We propose that the isolates that belonged to phenetic clusters E and F are members of a new species, Thermus oshimai; the type strain of T. oshimai is strain SPS17.
authors.affiliation: Department of Biochemistry, Queen Mary and Westfield College, London, United Kingdom. r.a.d.williams@qmw.ac.uk
authors.forename: R A
authors.initials: RA
authors.lastname: Williams
authors.forename: K E
authors.initials: KE
authors.lastname: Smith
authors.forename: S G
authors.initials: SG
authors.lastname: Welch
authors.forename: J
authors.initials: J
authors.lastname: Micallef
mesh_terms: D001394:Azores; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D007055:Iceland; D008969:Molecular Sequence Data; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013824:Thermus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S