Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin.
Isogenic mutants of Streptococcus gordonii DL1 (Challis) in which the genes encoding high-molecular-mass cell surface polypeptides CshA and/or CshB were inactivated were deficient in binding to four strains of Actinomyces naeslundii and two strains of Streptococcus oralis. Lactose-sensitive interactions of S. gordonii with A. naeslundii ATCC 12104 and PK606 were associated with expression of cshA but not of cshB. Lactose-insensitive interactions of S. gordonii with A. naeslundii T14V and WVU627, and with S. oralis C104 and 34, were dependent on expression of cshA and cshB. S. gordonii DL1 cells bound to immobilized human fibronectin (Fn), but not to soluble Fn, in a dose-dependent manner, and binding was noninhibitable by heparin. S. gordonii cshA and cshB mutants were also deficient in binding to immobilized human Fn. Antibodies to an NH2-terminal nonrepetitive region (amino acid residues 42 to 886) of recombinant CshA inhibited binding of S. gordonii DL1 cells to A. naeslundii T14V and PK606 and to immobilized Fn. Conversely, antibodies to an amino acid repeat block segment of the COOH-terminal domain (amino acid residues 2026 to 2508) were not inhibitory to adherence. Assays using CshA-specific antibodies revealed that surface expression of CshA was reduced in cshB mutants. The results suggest that CshA acts as a multifunctional adhesin in S. gordonii and that major adhesion-mediating sequences are specified within the nonrepetitive NH2-terminal region of the polypeptide.
McNab
R
R
Department of Oral Biology and Oral Pathology, University of Otago, Dunedin, New Zealand.
Holmes
A R
AR
Clarke
J M
JM
Tannock
G W
GW
Jenkinson
H F
HF
D000818:Animals; D000918:Antibody Specificity; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins; D009055:Mouth / Q000382:microbiology*; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits
D001426:Bacterial Proteins; C079041:CshA protein, Streptococcus gordonii; D005353:Fibronectins; D008565:Membrane Proteins; D010446:Peptide Fragments