Secondary structure of the panhandle RNA of influenza virus A studied by NMR spectroscopy.
The double-stranded panhandle structure of the influenza virus RNA is important for replication, transcription and packaging into the virion of the virion RNA. The solution structure of a 34 nt RNA which contains the conserved panhandle sequences has been investigated by one- and two-dimensional NMR spectroscopy. The partially complementary 5'- and 3'-ends of the RNA form a double helical structure which is, on average, close to A-form. The stem contains bulges at nucleotides A10, A12 and C26. In between these bulges, C11 and G25 form a Watson-Crick base pair. The structural features of the panhandle provide a framework for the explanation of mutational analysis and for a better understanding of RNA-polymerase interactions.
Cheong
H K
HK
Department of Chemistry, Korea Advanced Institute of Science and Technology, Yusung-gu, Taejon. haekap@reflex.kaist.ac.kr
Cheong
C
C
Choi
B S
BS
D009980:Influenza A virus / Q000737:chemistry / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry*
D012367:RNA, Viral