title: Radioadaptive response: efficient repair of radiation-induced DNA damage in adapted cells.
abstract: To verify the hypothesis that the induction of a novel, efficient repair mechanism for chromosomal DNA breaks may be involved in the radioadaptive response, the repair kinetics of DNA damage has been studied in cultured Chinese hamster V79 cells with single-cell gel electrophoresis. The cells were adapted by priming exposure with 5 cGy of gamma-rays and 4-h incubation at 37 degrees C. There were no indication of any difference in the initial yields of DNA double-strand breaks induced by challenging doses from non-adapted cells and from adapted cells. The rejoining of DNA double-strand breaks was monitored over 120 min after the adapted cells were challenged with 5 or 1.5 Gy, doses at the same level to those used in the cytogenetical adaptive response. The rate of DNA damage repair in adapted cells was higher than that in non-adapted cells, and the residual damage was less in adapted cells than in non-adapted cells. These results indicate that the radioadaptive response may result from the induction of a novel, efficient DNA repair mechanism which leads to less residual damage, but not from the induction of protective functions that reduce the initial DNA damage.
authors.affiliation: Biology Division, Kyoto University of Education, Japan. ikushima@wsml.kyokyo-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Ikushima
authors.forename: H
authors.initials: H
authors.lastname: Aritomi
authors.forename: J
authors.initials: J
authors.lastname: Morisita
mesh_terms: D000222:Adaptation, Physiological / Q000528:radiation effects*; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004586:Electrophoresis / Q000379:methods; D005720:Gamma Rays; D007700:Kinetics; D008954:Models, Biological; D013997:Time Factors
chemical_list: D004247:DNA