Determination of insulin in single pancreatic cells by capillary electrophoresis and laser-induced native fluorescence.
Development of a method for the determination of insulin based on capillary electrophoresis with laser-induced native fluorescence detection is described. Under optimal conditions, insulin as low as 73 amol can be detected with a good signal-to-noise ratio (S/N = 10 peak-to-peak). Application of this method for the determination of insulin content in single cells from the insulin-secreting cell lines RINm5F and beta TC3 is demonstrated. Non-bonded poly(ethylene oxide)-coated and bare capillaries are evaluated for this purpose, with the latter found to be more suitable for single-cell analysis.
Tong
W
W
Department of Chemistry, Iowa State University, Ames 50011, USA.
Yeung
E S
ES
D000818:Animals; D002460:Cell Line; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000737:chemistry* / Q000166:cytology; D007834:Lasers; D051379:Mice; D051381:Rats
D007328:Insulin