HPRT mutant frequencies and detection of large deletions by multiplex-PCR in human lymphocytes of vinyl chloride exposed and non-exposed populations.
The frequency of hypoxanthine guanine phosphoribosyltransferase (HPRT) mutations was determined in human T-lymphocytes isolated from peripheral blood of three populations: (1) 24 employees occupationally exposed to vinyl chloride monomer; (2) 23 healthy non-exposed matched control individuals and (3) 41 regular blood donors. In addition, mutant clones of all studied groups were examined by multiplex-PCR for visible abnormalities of the gene (large deletions). Surprisingly, the mutation frequency of all three investigated populations was approximately the same (7-8 x 10(-6)). However, great differences occurred for the spectrum of mutants. Among the mutant clones of the non-exposed healthy individuals only 6% (blood donors) and 11% (matched control group) showed large deletions. The corresponding percentage of large deletions in the occupationally exposed group was, at 21%, much higher.
Hüttner
E
E
Institute of Plant Genetics and Crop Plant Research, Population Monitoring Unit, Gatersleben, Germany.
Holzapfel
B
B
D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects; D002273:Carcinogens / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000235:genetics*; D002999:Clone Cells / Q000502:physiology; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D016296:Mutagenesis / Q000187:drug effects; D016273:Occupational Exposure / Q000009:adverse effects*; D016133:Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D014752:Vinyl Chloride / Q000009:adverse effects*
D000395:Air Pollutants, Occupational; D002273:Carcinogens; D007041:Hypoxanthine Phosphoribosyltransferase; D014752:Vinyl Chloride