Cleavage and development in cultured preimplantation mouse embryos exposed to lidocaine.
Two-cell preimplantation mouse embryos were exposed in vitro to lidocaine (0 to 1,000 micrograms/mL) for 72 h to determine the effects of this anesthetic on subsequent cleavage and development during prolonged exposures. Embryonic development was monitored each 24 h for 3 d. Lidocaine adversely affected the in vitro development of the mouse embryos, altering the distribution of the development stages at the evaluated culture times. The percentage of two-cell embryos that cleaved and developed to more advanced stages was decreased by the exposure to lidocaine. After 24 h of culture, two-cell embryos were arrested before completion of cellular division; this occurred in 30% of the embryos at concentrations of 10 to 100 micrograms/mL and in 73.2% of the embryos with 1,000 micrograms/mL. After 48 h the blastomeres of the arrested embryos began to degenerate, showing lysis or fragmentation. At the lowest concentration, 14.9% of the arrested embryos exhibited the capacity to recover. These embryos continued their cleavage and normal development towards blastocyst formation. The cytotoxic effect and arrest at the two-cell stage were observed in a dose-dependent manner after 72 h of culture. We conclude that sensitivity to lidocaine embryotoxicity occurs during a window at the two-cell stage.
Del Valle
L J
LJ
Laboratory of Reproduction and Development Biology, Faculty of Biological Sciences, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Orihuela
P A
PA
D000779:Anesthetics, Local / Q000633:toxicity*; D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008012:Lidocaine / Q000633:toxicity*; D051379:Mice
D000779:Anesthetics, Local; D008012:Lidocaine