Estradiol conjugated to BSA releases oxytocin from synaptosome-containing homogenates from the medial preoptic area-hypothalamus.
Estradiol conjugated to bovine serum albumin at position 6(E-6-BSA) released oxytocin (OT) from homogenates of the medial preoptic area and medial hypothalamus (MPOA-MH) within minutes of its superfusion. Using a superfusion system in which synaptosome-containing homogenates were layered onto acrodiscs maintained at 37 degrees C, we have found that E-6-BSA (100 ng/microliters) superfusions significantly elevated OT release within minutes. In contrast, superfusion of the same concentration of BSA or progesterone-3-BSA (P-3-BSA) had no effect on OT release. While superfusing homogenates with augmented levels of K+ had no effect on OT release itself, superfusing E-6-BSA with these concentrations of K+ consistently increased OT release. This is the first demonstration that E-6-BSA increases OT release in a nucleus-free medium.
Caldwell
J D
JD
Department of Pharmaceutical Sciences, North Dakota State University, Fargo, USA.
Morris
M A
MA
Walker
C H
CH
Carr
R B
RB
Faggin
B M
BM
Mason
G A
GA
D000818:Animals; D004958:Estradiol / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D009479:Neuropeptides / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism*; D011301:Preoptic Area / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D009479:Neuropeptides; D012710:Serum Albumin, Bovine; D004958:Estradiol; D010121:Oxytocin