title: Lack of the extracellular 19-kilodalton fibrinogen-binding protein from Staphylococcus aureus decreases virulence in experimental wound infection.
abstract: A mutant deficient for the 19-kDa extracellular fibrinogen-binding protein (Fib) from Staphylococcus aureus has been constructed. The gene was inactivated by allele replacement. A 2.0-kb fragment from transposon Tn4001 carrying the gene for gentamicin resistance was inserted into the gene encoding Fib (fib). The genotype was verified by PCR analysis, and the loss of Fib was demonstrated by Western blotting (immunoblotting). The mutation has not altered the ability of the strain to bind to fibrinogen or fibronectin compared with that of the isogenic parental strain, FDA486. The mutant, designated K4.3, was compared with strain FDA486 in a wound infection model in rats. Sixty-eight percent of the rats challenged with parental strain FDA486 developed severe clinical signs of wound infection, whereas only 29% of the animals challenged with isogenic mutant K4.3 showed severe symptoms (P < 0.01). The weight loss of animals infected with the wild type was also significantly different from that of animals infected with the mutant strain. The result demonstrates that the extracellular 19-kDa fibrinogen-binding protein from S. aureus contributes to the virulence in wound infection and delays the healing process.
authors.affiliation: Department of Microbiology, Immunology, Pathology and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Palma
authors.forename: S
authors.initials: S
authors.lastname: Nozohoor
authors.forename: T
authors.initials: T
authors.lastname: Schennings
authors.forename: A
authors.initials: A
authors.lastname: Heimdahl
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
mesh_terms: D000483:Alleles; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence; D014946:Wound Infection / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D005340:Fibrinogen