title: The presence of calbindin in rat cortical neurons protects in vitro from oxydative stress.
abstract: Free radicals are highly reactive chemicals containing an unpaired electron and are normally produced by the cellular metabolism. The oxydative stress is defined as a lack of balance between the production of free radicals and the activity of antioxydant metabolites. It induces cellular damages to lipids, proteins and membranes. Abnormal calcium metabolism can be a consequence of oxydative stress leading to increased intracellular concentrations. Calbindin D28K is a calcium binding protein which could have a neuroprotective action against various cellular insults. In this study rat cortical cell cultures were exposed during various times and at different concentrations to the couple Xanthine/Xanthine oxydase (XA/XO), which produces the superoxyde radical O2-.. Neuronal survival revealed that XA/XO is toxic for cortical cell cultures. The Calbindin D28K immunocytochemical study shows that the percentages of Calbindin positive cells are greater in surviving neurons following the XA/XO exposure compared to controls. There is a time-dependent and a dose-dependent relation between the number of surviving neurons and the percentage of Calbindin positive neurons. These results suggest that the presence of cytosolic neuronal Calbindin D28k is associated with a greater resistance to oxydative stress.
authors.affiliation: Cellular Neurobiology Unit, Laboratory of Histology, Faculty of Medicine, Limoges, France.
authors.forename: J
authors.initials: J
authors.lastname: Hugon
authors.forename: F
authors.initials: F
authors.lastname: Hugon
authors.forename: F
authors.initials: F
authors.lastname: Esclaire
authors.forename: M
authors.initials: M
authors.lastname: Lesort
authors.forename: A G
authors.initials: AG
authors.lastname: Diop
mesh_terms: D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009410:Nerve Degeneration / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000633:toxicity; D014970:Xanthines / Q000633:toxicity
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D014970:Xanthines; D013481:Superoxides; D014969:Xanthine Oxidase