title: A Brevibacterium linens pRBL1 replicon functional in Corynebacterium glutamicum.
abstract: Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pXZ10142 (C. glutamicum) and pAL5000 (Mycobacterium fortuitum). The 310-aa protein also shows significant homology to proteins of pColE5-099 (Shigella sonnei) and pJD1 (Neisseria gonorrhoea). At least one of these proteins, the Rep protein of pColE5-099, is known to be involved in theta replication.
authors.affiliation: Institut de Génétique et Microbiologie URA, Université de Paris-Sud, Orsay, France.
authors.forename: S
authors.initials: S
authors.lastname: Ankri
authors.forename: I
authors.initials: I
authors.lastname: Bouvier
authors.forename: O
authors.initials: O
authors.lastname: Reyes
authors.forename: F
authors.initials: F
authors.lastname: Predali
authors.forename: G
authors.initials: G
authors.lastname: Leblon
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001951:Brevibacterium / Q000235:genetics*; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D005822:Genetic Vectors / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000502:physiology*; D012093:Replicon / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins