Immunomodulating ability of galactoside-specific lectin standardized and depleted mistletoe extract.
Commercially available mistletoe extract standardized for the galactoside-specific lectin (ML-1; Eurixor) and a chromatographically ML-1-depleted preparation (same charge no. and composition of remaining components) were tested for their immunomodulating potency. In BALB/c-mice, regular subcutaneous administration of the optimal immunomodulating dosage (1 ng ML-1/kg body weight) could be shown to induce no influence on spleen weight, a non-significant increase of thymus weight, a significant increase of thymocyte, peritoneal macrophage, peripheral blood leukocyte, lymphocyte and monocyte and monocyte counts, and a significant decrease of peripheral blood granulocyte counts. Administration of analogue volumes (concentrations) of ML-1-depleted extract, however, did not induce any immunopotentiation. Accordingly, it may be assumed, that the galactoside-specific lectin (ML-1) represents the main immunomodulating component in commercially available mistletoe extracts.
Beuth
J
J
Institute of Medical Microbiology and Hygiene, University of Cologne, Germany.
Stoffel
B
B
Ko
H L
HL
Jeljaszewicz
J
J
Pulverer
G
G
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D005697:Galactosides / Q000737:chemistry*; D007155:Immunologic Factors / Q000494:pharmacology; D037102:Lectins / Q000737:chemistry*; D018655:Lymphocyte Count / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D014784:Mistletoe / Q000737:chemistry*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D037121:Plant Lectins; D010946:Plants, Medicinal; D013154:Spleen / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects
D000276:Adjuvants, Immunologic; D005697:Galactosides; D007155:Immunologic Factors; D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins