Further studies on the identification of the phagocytosis receptor of rat retinal pigment epithelial cells.
We have previously produced a polyclonal antiserum (R1S5) against a plasma membrane-enriched fraction of rat retinal pigment epithelial (RPE) cells which inhibits the phagocytosis of photoreceptor outer segments (OS) by these cells. This antiserum has now been used to purify a subset of RPE membrane glycoproteins. Using a combination of lectin affinity chromatography, and chromatography on an affinity column made with R1S5-IgG, we have enriched an RPE membrane extract about 100-fold. This enriched extract contains only 12 components, all of which are glycoproteins, and retains the ability to adsorb out the inhibitory activity of antiserum R1S5. This shows that one or more of these glycoproteins recognizes an inhibitory IgG in R1S5 and suggests that one or more of these glycoproteins may participate in the phagocytosis of OS by RPE cells, possibly as the phagocytosis receptor. We have performed N-terminal microsequencing of seven of these glycoproteins: four of the seven, with Mrs of 34, 36, 51 and 55 kDa, show no sequence homology to any known proteins.
Hall
M O
MO
Jules Stein Eye Institute, UCLA School of Medicine 90095-7008, USA.
Burgess
B L
BL
Abrams
T A
TA
Ershov
A V
AV
Gregory
C Y
CY
D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence; D010587:Phagocytosis / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012374:Rod Cell Outer Segment / Q000378:metabolism
D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface