The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.
As part of the European BIOTECH programme, the nucleotide sequence of a 16691 bp fragment from the left arm of chromosome IV of Saccharomyces cerevisiae has been deduced. Analysis of the sequence reveals the presence of 13 open reading frames (ORFs) larger than 100 codons. five of these were previously identified as genes DUN1, PMT1, PMT5, SRP14 and DPR1. One putative protein, D2371p, contains an ATP-GTP binding site, and shares homology to the ArsA component of an Escherichia coli arsenical pump. No significant homology to any known protein has been found for the other ORFs. D2378p contains a zinc finger domain.
Boskovic
J
J
Centro de Biología Molecular Severo Ochoa, CSIC, Madrid, Spain.
Soler-Mira
A
A
García-Cantalejo
J M
JM
Ballesta
J P
JP
Jiménez
A
A
Remacha
M
M
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D001330:Electronic Data Processing; D005656:Fungal Proteins / Q000235:genetics*; D008364:Mannosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018271:Signal Recognition Particle / Q000235:genetics*; D014166:Transferases / Q000235:genetics*
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; C089642:SRP14 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D018271:Signal Recognition Particle; D014166:Transferases; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase; C070038:RAM1 protein, S cerevisiae; D011494:Protein Kinases; C104169:DUN1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases