Heteromultimeric CLC chloride channels with novel properties.
The skeletal muscle chloride channel CLC-1 and the ubiquitous volume-activated chloride channel CLC-2 belong to a large gene family whose members often show overlapping expression patterns. CLC-1 and CLC-2 are coexpressed in skeletal and smooth muscle and in the heart. By coexpressing CLC-1 and CLC-2 in Xenopus oocytes, we now show the formation of novel CLC-1/CLC-2 heterooligomers that yield time-independent linear chloride currents with a chloride-->bromide-->iodide selectivity sequence. Formation of heterooligomeric CLC channels increases the number and possible functions of chloride channels.
Lorenz
C
C
Center for Molecular Neurobiology, Hamburg University, Germany.
Pusch
M
M
Jentsch
T J
TJ
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D000090007:CLC-2 Chloride Channels; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D018118:Chloride Channels / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D005260:Female; D046911:Macromolecular Substances; D008564:Membrane Potentials / Q000187:drug effects; D005810:Multigene Family; D009124:Muscle Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D017354:Point Mutation; D055503:Protein Multimerization; D018075:RNA, Complementary; D014982:Xenopus laevis
D000873:Anthracenes; C086321:CLC-1 channel; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; D046911:Macromolecular Substances; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D018075:RNA, Complementary; C010030:9-anthroic acid