Limited influence of the mesothelium on the influx of monocytes into the peritoneal cavity.
We have investigated the role of human mesothelium in an in vitro model of peritonitis on the monocyte adherence to and migration across monolayers of peritoneal mesothelial cells. Monocytes adhere avidly to non-activated mesothelial cell monolayers; however, migration in this situation was minimal. Prestimulation of the monolayers with II-1 beta did not alter these results. Anti-CD18 and anti-VLA-4 mAbs used in combination had an additive inhibitory effect on monocytes adherence to resting or IL-1 beta-pretreated mesothelial cells. MCP-1 and TGF-beta are secreted by mesothelial cells. Both have a modest role in mesothelium-induced monocyte chemotaxis: mAbs against these cytokines had an additive inhibitory effect on the chemotaxis induced by supernatant from 24-h prestimulated mesothelial cells. Our results indicate that the mesothelium itself has a limited role in the influx of monocytes into the peritoneal cavity during the onset of peritonitis.
Zeillemaker
A M
AM
Department of Surgery, Diakonessen Hospital, Utrecht, Netherlands.
Mul
F P
FP
Van Papendrecht
A A
AA
Leguit
P
P
Verbrugh
H A
HA
Roos
D
D
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D019040:E-Selectin / Q000276:immunology; D004848:Epithelium / Q000502:physiology*; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007375:Interleukin-1 / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology*; D010538:Peritonitis / Q000503:physiopathology; D016201:Receptors, Lymphocyte Homing / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D019040:E-Selectin; D039041:Integrin alpha4beta1; D016023:Integrins; D007375:Interleukin-1; D016201:Receptors, Lymphocyte Homing; D016212:Transforming Growth Factor beta; D018799:Intercellular Adhesion Molecule-1