A cdc5+ homolog of a higher plant, Arabidopsis thaliana.
We cloned and characterized a cDNA corresponding to a cdc5+ homolog of the higher plant, Arabidopsis thaliana. The cDNA, named AtCDC5 cDNA, encodes a polypeptide of 844 amino acid residues. The amino acid sequence of N-terminal one-fourth region of the predicted protein bears significant similarity to that of Schizosaccharomyces pombe Cdc5 and Myb-related proteins. Overexpression of the AtCDC5 cDNA in S. pombe cells is able to complement the growth defective phenotype of a cdc5 temperature-sensitive mutant. These results indicate that the AtCDC5 gene is a plant counterpart of S. pombe cdc5+. This is the first report of a cdc5(+)-like gene in a multicellular organism. We also demonstrated that a recombinant AtCDC5 protein possesses a sequence specific DNA binding activity (CTCAGCG) and the AtCDC5 gene is expressed extensively in shoot and root meristems. In addition, we cloned a PCR fragment corresponding to the DNA binding domain of human Cdc5-like protein. These results strongly suggest that Cdc5-like protein exists in all eukaryotes and may function in cell cycle regulation.
Hirayama
T
T
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Shinozaki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers; D004330:Drosophila; D005816:Genetic Complementation Test; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000737:chemistry; D020598:Proto-Oncogene Proteins c-myb; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
C102354:ATCDC5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C085875:cdc5 protein, S pombe; D005982:Glutathione Transferase