title: Regulation of skeletal muscle metabolism in the lizard Dipsosaurus dorsalis by fructose-2,6-bisphosphate.
abstract: Changes in liver and skeletal muscle fructose-2,6-bisphosphate (Fru-2,6-P2) concentrations were compared during fasting, exercise, and recovery in the lizard Dipsosaurus dorsalis and in outbred mice (Mus musculus). We present the first correlative evidence that suggests that a decrease in the content of Fru-2,6-P2 may mediate elevated gluconeogenesis in lizard skeletal muscle. Contents of Fru-2,6-P2 in lizard gastrocnemius and red and white iliofibularis (IF) were significantly lower (as much as 55% in white IF) during recovery from exhaustive exercise than at rest. Recovery from exhaustive exercise had no significant effect on Fru-2,6-P2 concentrations in any mouse muscle examined. Fasting significantly depressed lizard and mouse liver Fru-2,6-P2 contents and decreased lizard red IF by over 84% from the fed condition. Lizard red and white muscle fiber bundles incubated in 20 mM lactate had significantly lower Fru-2,6-P2 (94 and 61% depression, respectively) than those incubated in 8.5 mM glucose. These results are consistent with the hypothesis that Fru-2,6-P2 acts as a signal for controlling gluconeogenesis in lizard skeletal muscle.
authors.affiliation: University of Colorado, Department of Environmental Population and Organismic Biology, Boulder 80309-0334, USA. Scholnic@samiam.colorado.edu
authors.forename: D A
authors.initials: DA
authors.lastname: Scholnick
authors.forename: T T
authors.initials: TT
authors.lastname: Gleeson
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D005215:Fasting; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism*; D007076:Iguanas / Q000378:metabolism*; D019344:Lactic Acid / Q000494:pharmacology; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D005082:Physical Exertion; D012146:Rest
chemical_list: D005635:Fructosediphosphates; D019344:Lactic Acid; C027652:fructose 2,6-diphosphate