Rickettsia africae sp. nov., the etiological agent of African tick bite fever.
We propose the name Rickettsia africae sp. nov. (with type strain Z9-Hu) for a distinct species of spotted fever group (SFG) rickettsiae that is the etiological agent of African tick bite fever in humans. This rickettsia has a distinct natural cycle and can be phenotypically distinguished from the other SFG rickettsiae by microimmunofluorescence serotyping, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and by Western blotting (immunoblotting). Genotypic differences between R. africae and the other SFG rickettsiae can be demonstrated by PCR restriction fragment length polymorphism analysis, pulsed-field gel electrophoresis, and sequencing of the 16S rRNA gene.
Kelly
P J
PJ
Department of Clinical Veterinary Studies, University of Zimbabwe, Harare.
Beati
L
L
Mason
P R
PR
Matthewman
L A
LA
Roux
V
V
Raoult
D
D
D000349:Africa; D015153:Blotting, Western; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005334:Fever / Q000209:etiology; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012281:Rickettsia / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D012282:Rickettsia Infections / Q000382:microbiology*; D012703:Serotyping; D017282:Tick-Borne Diseases / Q000209:etiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S