Antibiotic GE37468A: a new inhibitor of bacterial protein synthesis. III. Strain and fermentation study.
GE37468A is a new thiazolyl peptide antibiotic acting on Elongation Factor Tu, produced by fermentation of Streptomyces sp. ATCC 55365. The low (< 10 mg/liter) and poorly reproducible antibiotic productivity of the original soil isolate hampered the development of a scalable fermentation process. A tenfold increase of GE37468A productivity was achieved by selection of spontaneous morphochromatic variants, which appeared at high frequency in the wild type population. The higher producing ATCC 55365/O/5 colony was tyrosinase defective and blocked in the aerial mycelium differentiation (Amy-Mel- phenotype). The fermentation process of this stable and purified strain was rapidly optimized to achieve a further fourfold improvement in a fed-batch process.
Marinelli
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F
Lepetit Research Center, Gerenzano (VA) Italy.
Gastaldo
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L
Toppo
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G
Quarta
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C
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation / Q000502:physiology*; D010456:Peptides, Cyclic / Q000378:metabolism* / Q000494:pharmacology; D010641:Phenotype; D011500:Protein Synthesis Inhibitors / Q000378:metabolism / Q000494:pharmacology; D012988:Soil Microbiology; D013302:Streptomyces / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; C096599:GE 37468 A; D010456:Peptides, Cyclic; D011500:Protein Synthesis Inhibitors; D013844:Thiazoles