A Mycobacterium tuberculosis gene cluster encoding proteins of a phosphate transporter homologous to the Escherichia coli Pst system.
We report the cloning and sequencing of three M. tuberculosis genes encoding proteins homologous to E. coli PstA, PstC and PstB. They are tentatively called pstA-2, pstC-1 and pstB. They encode proteins of 302, 336 and 275 amino acids, respectively. In E. coli, PstB is the ATP binding component and PstA/PstC are the two hydrophobic subunits of a phosphate permease belonging to the family of ABC (ATP-binding cassette) transporters. In mycobacteria, PstS-1, the phosphate binding subunit (Andersen and Hansen, 1989), is encoded by a gene directly surrounded by pstB, pstC-1 and pstA-2 within a potential operon (pstB, pstS-1, pstC-1, pstA-2). Phosphate uptake by whole, suspension grown, M. bovis BCG cells was measured and could be inhibited by a monoclonal antibody directed against the PstS-1 subunit, suggesting that these genes encode subunits of a functional mycobacterial phosphate permease.
Braibant
M
M
Department of Virology, Institut Pasteur, Brussels/Belgium.
Lefèvre
P
P
de Wit
L
L
Peirs
P
P
Ooms
J
J
Huygen
K
K
Andersen
A B
AB
Content
J
J
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001692:Biological Transport; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009163:Mycobacterium bovis / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D010710:Phosphates / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D010710:Phosphates; C489729:PstA protein, E coli; C102499:PstA-2 protein, Mycobacterium tuberculosis; C489730:PstC protein, E coli; C078409:PstC protein, bacteria; C102501:PstC-1 protein, Mycobacterium tuberculosis; C078408:phosphate-specific transport protein A, bacteria