Cloning and characterization of a repetitive DNA detected by HindIII in the genome of Raja montagui (Batoidea, Chondrichthyes).
A repetitive HindIII fragment of DNA from Raja montagui (Rajiformes) was cloned and sequenced for the first time in cartilaginous fishes. This element, which comprises approximately 5% of the whole genome of the spotted ray, is absent in long tandem arrays, being typical of satellite DNA. It appeared constituted by 311 AT-rich bp (61%). The clone was hybridized to the genomic DNA of species with varying phyletic distances, revealing a high degree of conservation.
Rocco
L
L
Department of Evolutionary and Comparative Biology, University of Naples, Federico II, Italy.
Stingo
V
V
Bellitti
M
M
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000302:isolation & purification; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012860:Skates, Fish / Q000235:genetics*
D004276:DNA, Satellite; D015247:Deoxyribonuclease HindIII