High-performance liquid chromatographic determination of phenol, 4-nitrophenol, beta-naphthol and a number of their glucuronide and sulphate conjugates in organ perfusate.
This paper describes a simple and more sensitive reversed-phase HPLC method for the quantification of phenol, 4-nitrophenol and beta-naphthol and some of their glucuronide and sulphate conjugates in aqueous solution and liver perfusate buffer. Methanol-water mobile phases with ion-pairing agents for each phenolic group are detailed. The assay showed good recovery, accuracy and precision and is suitable for the quantification of these phenolic compounds in liver perfusion experiments.
Thompson
M J
MJ
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Ballinger
L N
LN
Cross
S E
SE
Roberts
M S
MS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005965:Glucuronates / Q000032:analysis; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009284:Naphthols / Q000032:analysis* / Q000493:pharmacokinetics; D009596:Nitrophenols / Q000032:analysis* / Q000493:pharmacokinetics; D010636:Phenols / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000032:analysis
D005965:Glucuronates; D009284:Naphthols; D009596:Nitrophenols; D010636:Phenols; D013431:Sulfates; C028405:2-naphthol; C024836:4-nitrophenol