The effect of temperature and growth rate on the susceptibility of Listeria monocytogenes to environmental stress conditions.
The effect of growth temperature and growth rate on the susceptibility to heat and pH stress were investigated in Listeria monocytogenes grown in continuous culture where these two growth variables could be varied independently of each other, and in batch culture. After growth at 30 degrees C or 10 degrees C at constant growth rate, or at 30 degrees C at different growth rates, cells did not differ in their resistance to heat at 55 degrees C. Cells grown at 30 degrees C were more resistant to acid stress at pH 2.5 than cells grown at the same growth rates at 10 degrees C. Cells grown at low growth rate at 30 degrees C gave greater resistance to acid stress than those grown at high growth rate. Growth temperature and growth rate had independent effects on the susceptibility of L. monocytogenes to acid stress conditions. This may have implications for the survival of L. monocytogenes in acidic foods.
Patchett
R A
RA
Institute of Food Research, Reading, Berks, UK.
Watson
N
N
Fernandez
P S
PS
Kroll
R G
RG
D005516:Food Microbiology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D008088:Listeriosis / Q000209:etiology; D013312:Stress, Physiological; D013696:Temperature