title: Altered actin polymerization dynamics in various malignant cell types: evidence for differential sensitivity to cytochalasin B.
abstract: Using the DNase I inhibition assay, fluorimetric measurements, and immunoblot analysis, we studied quantitatively changes in the actin polymerization dynamics in primary cultures of normal and malignant human lymphocytes, normal human endometrial cells, and in various leukemic and endometrial adenocarcinoma cell lines. The G/total-actin ratio of malignant cells was found to be 1.37 to 1.81-fold higher compared to normal cells, indicating that malignant cells express reduced amounts of polymerized actin. The above findings were corroborated by fluorescence measurements of the amounts of rhodamine-phalloidin-labeled F-actin in normal and neoplastic cells, which showed significantly lower F-actin content in malignant cell preparations. Moreover, the total actin content, as quantitated by the DNase I inhibition assay and by immunoblot analysis, was found to be significantly decreased in the primary cultures of malignant human lymphocytes and endometrial cells when compared to the total actin levels in corresponding normal cells. Proliferation and viability measurements of normal and neoplastic cells in culture, treated equally with cytochalasin B (CB), revealed an increased susceptibility of malignant cells to this anticytoskeletal agent. This was not due to increased CB incorporation in neoplastic cells, as indicated by 3H-CB uptake experiments. In addition, fluorescence microscopy, in the presence of graded concentrations of CB, showed destabilization of microfilaments in the poorly differentiated endometrial adenocarcinoma HEC-50 cells, compared to the well-differentiated Ishikawa cells. In conclusion, all investigated malignant cells are characterized by: (a) higher G/total-actin ratio; (b) decreased F- and total-actin content; and (c) lower resistance to CB treatment. These quantitatively determined parameters may represent potential biochemical indicators reflecting malignant transformation. Moreover, it seems worthwhile to explore whether or not the differential sensitivity of malignant cells to anticytoskeletal drugs may provide a valuable approach to the manipulation of malignant cells.
authors.affiliation: Department of Biochemistry, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Stournaras
authors.forename: E
authors.initials: E
authors.lastname: Stiakaki
authors.forename: S B
authors.initials: SB
authors.lastname: Koukouritaki
authors.forename: P A
authors.initials: PA
authors.lastname: Theodoropoulos
authors.forename: M
authors.initials: M
authors.lastname: Kalmanti
authors.forename: Y
authors.initials: Y
authors.lastname: Fostinis
authors.forename: A
authors.initials: A
authors.lastname: Gravanis
mesh_terms: D000199:Actins / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016889:Endometrial Neoplasms / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006801:Humans; D007938:Leukemia / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D011108:Polymers; D003571:Cytochalasin B