Multiresidue method for confirmation of macrolide antibiotics in bovine muscle by liquid chromatography/mass spectrometry.
A particle beam/liquid chromatographic/mass spectrometric (PB/LC/MS) method capable of determining 5 macrolides in bovine muscle is described. Spiramycin, tylosin, tilmicosin, erythromycin, and josamycin residues in bovine muscle are confirmed by reversed-phase LC/MS incorporating gradient elution. Macrolides are extracted from tissue with chloroform, and the extract is purified with a diol solid-phase extraction cleanup. The 5 macrolides are identified by negative and positive chemical ionization with selective ion monitoring of 2 ions in each mode. The procedure confirms the presence of each macrolide at 50 micrograms/kg in spiked samples.
Delépine
B
B
Laboratoire des Médicaments, Centre National d'Etudes Vétérinaires et Alimentaires, Fougères, France.
Hurtaud-Pessel
D
D
Sanders
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis; D004917:Erythromycin / Q000032:analysis; D015570:Josamycin / Q000032:analysis; D018942:Macrolides; D013058:Mass Spectrometry / Q000379:methods; D009132:Muscles / Q000737:chemistry*; D015572:Spiramycin / Q000032:analysis; D015645:Tylosin / Q000031:analogs & derivatives / Q000032:analysis
D000900:Anti-Bacterial Agents; D018942:Macrolides; D004917:Erythromycin; D015572:Spiramycin; D015570:Josamycin; C052319:tilmicosin; D015645:Tylosin