title: A novel type of pyridine nucleotide-disulfide oxidoreductase is essential for NAD+- and NADPH-dependent degradation of epoxyalkanes by Xanthobacter strain Py2.
abstract: Epoxide degradation in cell extracts of Xanthobacter strain Py2 has been reported to be dependent on NAD+ and dithiols. This multicomponent system has now been fractionated. A key protein encoded by a DNA fragment complementing a Xanthobacter strain Py2 mutant unable to degrade epoxides was purified and analyzed. This NADP-dependent protein, a novel type of pyridine nucleotide-disulfide oxidoreductase, is essential for epoxide degradation. NADPH, acting as the physiological cofactor, replaced the dithiols in epoxide conversion.
authors.affiliation: Division of Industrial Microbiology, Department of Food Science, Wageningen Agricultural University, The Netherlands. jelto.swaving@algemeen.im.wau.nl
authors.forename: J
authors.initials: J
authors.lastname: Swaving
authors.forename: J A
authors.initials: JA
authors.lastname: de Bont
authors.forename: A
authors.initials: A
authors.lastname: Westphal
authors.forename: A
authors.initials: A
authors.lastname: de Kok
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D000595:Amino Acid Sequence; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics; D004852:Epoxy Compounds / Q000378:metabolism*; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D000473:Alkanes; D004852:Epoxy Compounds; D013438:Sulfhydryl Compounds; D009243:NAD; D009249:NADP; D010088:Oxidoreductases; C021551:mercuric reductase; D009247:NADH, NADPH Oxidoreductases; C102540:pyridine nucleotide-disulfide oxidoreductase ORF3; D008058:Dihydrolipoamide Dehydrogenase; C009068:propylene oxide