The organization of the human gene NCX1 encoding the sodium-calcium exchanger.
The intron-exon organization of the human Na/Ca-exchanger gene NCX1 and of the N-terminal half of the related gene NCX2 has been determined. The NCX1 gene consists of 12 exons spread over 200 kb on chromosome 2 close to STS D2S2328 and encodes a 6.2-kb transcript. Both NCX1 and NCX2 feature an unusual 1.8-kb exon, containing two-thirds of the protein coding sequence and a similar area of the coding sequences split into several small exons, displaying tissue-specific alternative splicing. The similar intron positions in the "cardiac" (NCX1) and "brain" (NCX2) mammalian exchanger genes suggest their origin from the recent duplication and translocation of a common ancestral gene, a putative precursor of which has been identified in the nematode Caenorhabditis elegans.
Kraev
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A
Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.
Chumakov
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I
Carafoli
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011188:Potassium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D019831:Sodium-Calcium Exchanger; D012964:Sodium; D011188:Potassium