2-Chlorobenzoic acid and 2,5-dichlorobenzoic acid metabolism by crude extracts of Pseudomonas sp. CPE2 strain.
Crude extracts of Pseudomonas sp. CPE2 strain, which is capable of growing on 2-chlorobenzoic acid (2-CBA) and 2,5-dichlorobenzoic acid (2,5-dCBA) in the absence of other carbon sources, were found to be capable of bioconverting 2-CBA and 2,5-dCBA to catechol and 4-chlorocatechol, respectively, by a reaction requiring molecular oxygen and exogenous NADH. Extracts obtained from 2-CBA-grown cells in the presence of 2-CBA and from 2,5-dCBA-grown cells in the presence of 2,5-dCBA were found to have activities similarly influenced by the assay parameters pH, temperature, and by concentration of oxygen, protein, Fe2+, FAD and NADH in the assay medium. In addition, the activity of the two crude extracts in the presence of 2-CBA or 2,5-dCBA was described by very similar Michaelis-Menten kinetic parameters. These observations led to the speculation that a unique broad-spectrum chlorobenzoate 1,2-dioxygenase catalyses the 2-CBA and 2,5-dCBA metabolism both in 2-CBA- and 2,5-dCBA-grown CPE2 cells.
Fava
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Department of Applied Chemistry and Material Science, University of Bologna, Italy. DICHIASM1@INGBO1.CINECA.IT
Baldoni
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Marchetti
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D001673:Biodegradation, Environmental; D002723:Chlorobenzoates / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*
D002723:Chlorobenzoates; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; C060415:2-chlorobenzoic acid; D007501:Iron; D010105:Oxygenases; C073069:2,5-dichlorobenzoic acid