Long-term culture of avian embryonic cells in vitro.
The pH of the embryonic blood, one of the most important environmental factors for embryonic cells, was found to range from 8.1 to 8.5 in chick embryos until 108 h after incubation. Based on these results, the culture medium adjusted to pH 8.0 was used to culture embryonic chick and quail cells. They were easily subcultured for a long period of time at pH 8.0. This pH culture condition may have wide application for manipulating embryonic cells or tissues and establishing cell lines from avian embryos.
Kuwana
T
T
Research Development Corporation of Japan, Kumamoto. kuwana@infobears.or.jp
Hashimoto
K
K
Nakanishi
A
A
Yasuda
Y
Y
Tajima
A
A
Naito
M
M
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002470:Cell Survival; D002642:Chick Embryo; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011784:Quail / Q000196:embryology
D003470:Culture Media