title: [Creating a system of conjugated chromosome transfer and genetic mapping of Vibrio cholerae serogroup O139 chromosomes].
abstract: A conjugational gene transfer system consisting of donor and recipient strains has been developed for genetic analysis of Vibrio cholerae 0139 serogroup, a new cholera agent. Donor strains constructed using the Tn5-Mob carrying the origin of transfer (ori T) of plasmid RP4 and helper plasmid pRP4-4 were able to perform a directed transfer of chromosomal markers. Recipient strains carried mutations in auxotrophic genes as well as in virulence genes. Based on this gene transfer system, a genetic map of V. cholerae chromosome has been created showing the order of 17 gene markers. Relationship between the genetic structure of V. cholerae 0139 and 01 is discussed.
authors.forename: N I
authors.initials: NI
authors.lastname: Smirnova
authors.forename: G A
authors.initials: GA
authors.lastname: Eroshenko
authors.forename: G V
authors.initials: GV
authors.lastname: Chekhovskaia
authors.forename: L F
authors.initials: LF
authors.lastname: Livanova
authors.forename: N I
authors.initials: NI
authors.lastname: Davydova
authors.forename: S V
authors.initials: SV
authors.lastname: Shopyreva
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D018014:Gene Transfer Techniques; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements