title: Binding of extracellular matrix proteins to Aspergillus fumigatus conidia.
abstract: As detected by confocal immunofluorescence microscopy, binding of fibronectin and laminin appeared to be associated with the protrusions present on the outer cell wall layer of resting Aspergillus fumigatus conidia. Flow cytometry confirmed that binding of laminin to conidia was dose dependent and saturable. Laminin binding was virtually eliminated in trypsin-treated organisms, thus suggesting the protein nature of the binding site. Conidia were also able to specifically adhere to laminin immobilized on microtiter plates. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) with laminin and antilaminin antibody of whole conidial homogenates allowed identification, among the complex array of protein and glycoprotein species, of one polypeptide with an apparent molecular mass of 37 kDa which specifically interacts with laminin. The fact that binding of conidia to soluble or immobilized laminin or fibronectin was inhibited by fibronectin or laminin, respectively, suggests the existence of common binding sites for both ligands on the surface of conidia. Intact conidia were also able to adhere to type I and IV collagen immobilized on microtiter plates; adhesion was found to be dose dependent and saturable. Adhesion to immobilized type I and IV collagen was markedly inhibited by laminin and weakly inhibited by fibronectin. Coincubation of conidia with Arg-Gly-Asp (RGD) peptides caused a dose-dependent decrease in binding of cells to immobilized or soluble fibronectin, yet interaction of cells with soluble or immobilized laminin and type I and IV collagen remained unaffected. Interactions described here could be important in mediating attachment of the fungus to host tissues, thus playing a role in the establishment of the disease.
authors.affiliation: Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Gil
authors.forename: M C
authors.initials: MC
authors.lastname: Peñalver
authors.forename: J L
authors.initials: JL
authors.lastname: Lopez-Ribot
authors.forename: J E
authors.initials: JE
authors.lastname: O'Connor
authors.forename: J P
authors.initials: JP
authors.lastname: Martinez
mesh_terms: D001232:Aspergillus fumigatus / Q000502:physiology*; D002448:Cell Adhesion; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D005434:Flow Cytometry; D018613:Microscopy, Confocal; D011485:Protein Binding
chemical_list: D016326:Extracellular Matrix Proteins