[Allotransplantation of pancreatic islet cells and antinuclear antibodies].
Antinuclear antibodies were detected and studied by indirect immunofluorescence in the sera of patients with insulin-dependent diabetes mellitus (IDDM) subjected to allotransplantation of pancreatic islet cell culture (PICT). Continuous cell lines L-41 (derivative of J-96) and HEp-2, a sensitive substrate for the detection of antinuclear antibodies, were used after enzyme treatment. Antinuclear antibodies were detected in 19.0% of diabetics in comparison with 1.5% in healthy volunteers.PICT evidently did not influence these antibodies, although a rise of hemagglutinins against sheep red cells after PICT was previously observed. The data indicate that the nuclear antigens of HEp-2 and L-41 against which antinuclear antibodies are directed are different from those found in human fetal pancreatic islet cells which have been implanted. A possible NAg maturity deficiency in fetal tissue may be another cause of their failure to affect the antinuclear antibodies.
Galibin
O V
OV
Sherstnov
M Iu
MIu
Emanuél'
V L
VL
Kashchenko
V A
VA
Kofman
A V
AV
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology; D016381:Islets of Langerhans Transplantation; D008297:Male; D008875:Middle Aged
D000974:Antibodies, Antinuclear