Rapid liquid chromatographic determination of fatty acids as 2-nitrophenylhydrazine derivatives.
Direct derivatization of saponified fats and oils with 2-nitrophenylhydrazine hydrochloride produced corresponding fatty acid hydrazides without time-consuming sample cleanup and/or extraction steps. Hydrazides were injected directly into a liquid chromatograph. Improved isocratic separation was achieved within only 22 min for 29 saturated and mono- and polyunsaturated fatty acids (C8:0-C22:6), including cis-trans isomers and double-bond positional isomers. The technique has good quantitative accuracy while reducing analysis time, cost, and labor.
Miwa
H
H
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Yamamoto
M
M
D002853:Chromatography, Liquid / Q000379:methods; D005227:Fatty Acids / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods; D006834:Hydrazines; D007536:Isomerism; D010659:Phenylhydrazines; D015203:Reproducibility of Results
D005227:Fatty Acids; D006834:Hydrazines; D010659:Phenylhydrazines; C060574:2-nitrophenylhydrazine