[In vitro culture of growth cartilage and in situ reimplantation].
An original and reliable technique to culture growth plate chondrocytes was developed to obtain an abundant amount of mature and functional chondrocytes. Growth plates were provided from the epiphysis of 3 week old rabbits. The isolation of the chondrocytes was optimized by the use of trypsin and collagenase. The culture was realized according to the following conditions: seeding at 20,000 or 30,000/cm2 on type I collagen substrate and in Ham F12 medium without a supplementation of glucose or growth factors. After 7 days of culture, the implantation was to be carried out. Different implantation substrates were evaluated in vivo. Agar turned out to be the only substrate to provide strong and healthy chondrocytes 21 days after the grafting. In an other experimentation, the culture was implanted into surgically created defects in the growth plate area. In this case, the culture did not avoid the occurrence of an epiphysiodesis. However, the 6 weeks post operative histologic examination, showed that the implant remained viable, continued to maintain a proteoglycan rich matrix, and began to organize in ordered columns of mature chondrocytes.
Jouve
J L
JL
Service de Chirurgie pédiatrique et orthopédique II (Pr Bollini), Hôpital d'enfants de La Timone, Marseille.
D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix; D006132:Growth Plate / Q000166:cytology* / Q000601:surgery; D011817:Rabbits; D012092:Replantation; D013997:Time Factors