title: Immunological response to the Brucella abortus GroEL homolog.
abstract: Western blot (immunoblot) analysis of sera from cattle vaccinated with Brucella abortus S19 exhibit an elevated serologic response to Hsp62, the GroEL homolog (BaGroEL). Serologic screening of individual cows vaccinated with B. abortus S19 revealed no correlation between the immune response to BaGroEL and protection against a challenge with virulent organisms. The humoral immune response to BaGroEL was restricted to a region of the mature protein which mapped to amino acids 317 to 355 and may represent a useful diagnostic tool for monitoring exposure to B. abortus. Immunity to a challenge with virulent B. abortus S2308 was not observed in the BaGroEL vaccinated mouse model.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: L G
authors.initials: LG
authors.lastname: Adams
authors.forename: T A
authors.initials: TA
authors.lastname: Ficht
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015153:Blotting, Western; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D051379:Mice; D011817:Rabbits; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D018834:Chaperonin 60