Tetrameric triosephosphate isomerase from hyperthermophilic Archaea.
Triosephosphate isomerase (TIM) of the hyperthermophilic Archaea Pyrococcus woesei and Methanothermus fervidus have been purified to homogeneity. The enzymes from the two hyperthermophiles represent homo-tetramers of 100 kDa, contrary to all known bacterial and eukaryotic TIMs, which are dimers of 48-60 kDa. Molecular size determination of the TIM from the mesophilic methanogen Methanobacterium bryantii yielded the usual molecular mass of only 57 kDa, indicating that the tetrameric aggregation state does not represent an archaeal feature but rather correlates with thermoadaptation. A similar preference for higher protein aggregates in hyperthermophilic Archaea has previously been demonstrated for 3-phosphoglycerate kinases. The gene of the P. woesei TIM was cloned and sequenced. The archaeal TIM proved to be homologous to its bacterial and eukaryotic pendants. Most strikingly, the deduced protein sequence comprises only 224 residues and thus represents the shortest TIM sequence known as yet. Taking the three-dimensional structure of the eucaryal TIM as a basis, from the shortenings of the chain considerable rearrangements at the bottom of the alpha/beta barrel and at its functionally inactive flank are expected, which are interpreted in terms of the formation of new subunit contacts.
Kohlhoff
M
M
FB 9 Mikrobiologie, Universität Essen, Germany.
Dahm
A
A
Hensel
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000201:enzymology*; D001483:Base Sequence; D002645:Chickens; D002845:Chromatography; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017886:Durapatite; D019605:Euryarchaeota / Q000201:enzymology; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D017014:Methanobacterium / Q000201:enzymology; D008961:Models, Structural; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014305:Triose-Phosphate Isomerase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014345:Trypanosoma / Q000201:enzymology; D003313:Zea mays / Q000201:enzymology
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D017886:Durapatite; D014305:Triose-Phosphate Isomerase