Glutamate decarboxylase-67 messenger RNA expression in normal human basal ganglia and in Parkinson's disease.
Expression of glutamate decarboxylase-67 messenger RNA was examined in the basal ganglia of normal controls and of cases of Parkinson's disease using in situ hybridization histochemistry in human post mortem material. In controls glutamate decarboxylase-67 messenger RNA expression was detected in all large neurons in both segments of the globus pallidus and in three neuronal subpopulations in the striatum as well as in substantia nigra reticulata neurons and in a small sub-population of subthalamic neurons. In Parkinson's disease, there was a statistically significant decrease of 50.7% in glutamate decarboxylase-67 messenger RNA expression per neuron in the lateral segment of the globus pallidus (controls: mean 72.8 microns2 +/- S.E.M. 8.7 of silver grain/neuron, n = 12; Parkinson's disease: mean 35.9 microns2 +/- S.E.M. 9.7 of silver grain/neuron, n = 9, P = 0.01, Student's t-test). In the medial segment of the globus pallidus, there was a small, but non-significant decrease of glutamate decarboxylase-67 messenger RNA expression in Parkinson's disease (controls: mean 100.6 microns2 +/- S.E.M. 7.2 of silver grain/neuron, n = 11; Parkinson's disease: mean 84.8 microns2 +/- S.E.M. 13.0 of silver grain/neuron, n = 7, P = 0.1, Student's t-test). No significant differences in glutamate decarboxylase-67 messenger RNA were detected in striatal neuronal sub-populations between Parkinson's disease cases and controls. These results are the first direct evidence in humans that there is increased inhibitory drive to the lateral segment of the globus pallidus in Parkinson's disease, as suggested by data from animal models. We therefore provide theoretical support for current experimental neurosurgical approaches to Parkinson's disease.
Nisbet
A P
AP
Parkinson's Disease Society Brain Bank, London, U.K.
Eve
D J
DJ
Kingsbury
A E
AE
Daniel
S E
SE
Marsden
C D
CD
Lees
A J
AJ
Foster
O J
OJ
D000368:Aged; D000369:Aged, 80 and over; D001479:Basal Ganglia / Q000201:enzymology* / Q000473:pathology; D015152:Blotting, Northern; D015342:DNA Probes; D005260:Female; D005917:Globus Pallidus / Q000378:metabolism / Q000473:pathology; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000201:enzymology* / Q000473:pathology; D011699:Putamen / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology; D013787:Thalamic Nuclei / Q000378:metabolism / Q000473:pathology
D015342:DNA Probes; D012333:RNA, Messenger; D005968:Glutamate Decarboxylase