Determination of brequinar in rat plasma by direct deproteinization and reversed-phase high-performance liquid chromatography with ultraviolet absorbance detection.
A direct deproteinization method for the determination of brequinar in rat plasma by high-performance liquid chromatography (HPLC) has been developed. This assay avoids the use of dichloromethane, a known carcinogen, in an existing extraction method. Acetonitrile was used to denature plasma proteins and the supernatant was injected onto the HPLC column. Chromatographic separation of brequinar was conducted on a Biophase octyl column using a mixture of acetonitrile and 0.1 M phosphoric acid (50:50, v/v) as the mobile phase and detection of brequinar was by UV absorbance at 254 nm. The method has been validated in rat plasma over the concentration range of 0.05-50.00 micrograms/ml which is adequate for the determination of pharmacokinetics of brequinar in animals.
Dasgupta
S
S
Drug Metabolism and Pharmacokinetics Section, DuPont Merck Pharmaceutical Company, Newark, DE 19714, USA.
Timby
D M
DM
Lam
G N
GN
D000097:Acetonitriles / Q000494:pharmacology; D000818:Animals; D001713:Biphenyl Compounds / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D007166:Immunosuppressive Agents / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D011917:Rats, Inbred Lew; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D011798:Quinaldines
D000097:Acetonitriles; D001713:Biphenyl Compounds; D007166:Immunosuppressive Agents; C046943:brequinar; D011798:Quinaldines; C032159:acetonitrile