title: Quantitative comparison of casein and rapeseed proteolysis by pancreatin.
abstract: The problem of quantitative comparison of kinetic curves was solved for casein and rapeseed pancreatin hydrolysis in a membrane reactor, which ensured the measurement of proteolysis kinetics for the products with a molecular weight of less than 1000. Coordinates were derived which provided good linearization of kinetic curves and the determination of relative rate constants irrespective of reagent concentrations, E0/S0 ratio and time intervals of kinetic measurements. When the relative rate constants of the release of the individual amino acid residues in the low-weight proteolysis products were compared, trypsin-dependent constants (for Lys and Arg residues) were found to be two times less for rapeseed than for casein, and chymotrypsin-dependent constants (for Tyr and Phe residues) were approximately 1.3 times higher for rapeseed than for casein. Statistical analysis demonstrated that the distribution of constants was narrower for rapeseed than for casein. Differences between target (Arg, Lys, Tyr and Phe) and non-target constants of release in the form of peptides and free amino acids, or in the form of free amino acids only, were attributed on the differences in the peptide bond masking for casein and rapeseed proteins. Computer simulation of proteolysis kinetics was performed by PROTEOLYSIS program package to confirm the dependence of rate constant distribution on the state of masking.
authors.affiliation: Foodinform Ltd., Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Vorob'ev
authors.forename: G
authors.initials: G
authors.lastname: Parent
authors.forename: L
authors.initials: L
authors.lastname: Savoie
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001937:Brassica / Q000737:chemistry*; D002364:Caseins / Q000032:analysis / Q000737:chemistry*; D003198:Computer Simulation; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D008970:Molecular Weight; D010194:Pancreatin / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002364:Caseins; D010940:Plant Proteins; D010194:Pancreatin