Na,K-ATPase alpha- and beta-isoforms in the developing cochlea of the mouse.
Immunohistochemistry was used to investigate the presence of Na,K-ATPase alpha- and beta-subunits isoforms (alpha 1, alpha 2, alpha 3, beta 1 and beta 2) in the cochlea of the mouse at different ages between embryological day (E) 19 and postnatal day (P) + 30. alpha 1 was mainly found in the stria vascularis and in the spiral ligament; it increased steadily from p+4. These data correlates well with the morphological and electrophysiological maturation of the cochlea. alpha 3 predominated in the spiral ganglia and the cochlear nerve. This finding is well in accordance with reports that alpha 3 seems to be associated with the nervous system. The beta-subunit was found mainly in those tissues where staining of the alpha-subunit also was seen. Both subunits were localized in tissue regions where fluid regulation is expected to play an important role. For some isoforms, the expression pattern of Na,K-ATPase during development in the mouse is different from that in the rat. The expression of Na,K-ATPase and that of glucocorticoid receptors during development in the inner ear of the mouse show a similar pattern, which may indicate that glucocorticoid receptors could be involved in regulating the expression of Na,K-ATPase.
Erichsen
S
S
Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.
Zuo
J
J
Curtis
L
L
Rarey
K
K
Hultcrantz
M
M
D000818:Animals; D000918:Antibody Specificity; D003051:Cochlea / Q000201:enzymology* / Q000502:physiology; D003056:Cochlear Nerve / Q000201:enzymology / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005865:Gestational Age; D006706:Homeostasis; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D009925:Organ of Corti / Q000201:enzymology / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013136:Spiral Ganglion / Q000201:enzymology / Q000502:physiology; D013316:Stria Vascularis / Q000201:enzymology / Q000502:physiology
D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase