LP2, a differentiation-associated lipid-binding protein expressed in bovine lens.
A 13 kDa protein from bovine lens was identified and characterized by protein microsequencing and by rapid amplification of cDNA ends (RACE) PCR. Its complete sequence shows that this protein belongs to a family of fatty acid-binding proteins (FABPs), including myelin and adipocyte P2, that are associated with cellular differentiation. The bovine lens protein, designated LP2, shows very close similarity to human epidermal FABP (eFABP) and human eFABP was detected in human lens, suggesting that the two proteins might be orthologous. Reverse transcriptase-PCR (RT-PCR) was used to compare expression patterns of LP2 with those for actin and for the differentiation markers gamma B-crystallin and gamma s-crystallin in lens. Actin was most abundant in the relatively undifferentiated epithelial cells and decreased with lens cell differentiation. In contrast gamma B-crystallin and gamma s-crystallin were detected only in fibres (nuclear and cortical respectively). LP2 transcripts were detected most abundantly in fibre cells and apparently increased with cellular differentiation. Molecular modelling confirms that the sequence of LP2 fits the tertiary template of adipocyte P2 but reveals the presence of two close pairs of cysteine residues that might be susceptible to intramolecular disulphide bond formation under appropriate oxidizing conditions. LP2 is thus another potential target for oxidative stress during cataract formation in lens.
Jaworski
C
C
Section on Molecular Structure and Function, LMDB, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2730, USA.
Wistow
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D002352:Carrier Proteins; D005136:Eye Proteins; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger