Secondary structure content of the HDV ribozyme in 95% formamide.
The Hepatitis Delta Virus (HDV) ribozyme self-cleaving activity in 20 M formamide solutions is unique. Does this catalytic activity result from the conservation of its tertiary structure in 20 M formamide? We followed the ribozyme structure in formamide solutions by monitoring the amount of bound Ethidium Bromide (EB). We were able to measure the quantity of dye bound using time-resolved fluorescence spectroscopy, as an estimate of the ribozyme double helical content. This method, calibrated by using oligonucleotides with defined tertiary structure and denaturing solvents, parallels NMR and UV measurements as a function of temperature. Measurements with the HDV ribozyme lead to three conclusions: (a) both the precursor and product RNAs are structured to 24 M (95% w/w) formamide or 4 M H2O solutions which is equivalent to 4 M H2O; (b) the HDV ribozyme is the only RNA sequence investigated in this study that retains so much structure in formamide; and (c) DNA analogs of formamide resistant HDV ribozyme sequences lose their structure at less than 15 M formamide. Thus, the structural integrity of the HDV ribozyme is an intrinsic property of the RNA molecule and its sequence.
Duhamel
J
J
Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA.
Liu
D M
DM
Evilia
C
C
Fleysh
N
N
Dinter-Gottlieb
G
G
Lu
P
P
D001483:Base Sequence; D004996:Ethidium / Q000378:metabolism; D005559:Formamides / Q000494:pharmacology*; D003698:Hepatitis Delta Virus / Q000737:chemistry / Q000201:enzymology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000187:drug effects / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D005559:Formamides; D009841:Oligonucleotides; D016337:RNA, Catalytic; D012330:RNA, Double-Stranded; C031066:formamide; D004996:Ethidium