Genetic transformation of Vitreoscilla sp.
Of all the methods customarily used to transform E. coli we found only electroporation to be effective for transformation of the Gram-negative bacterium Vitreoscilla, yielding 5.10(5) transformants/microgram of plasmid DNA. The conditions used were close to those described for E. coli E. coli plasmids are stably maintained in Vitreoscilla. This is the first report of exogenous DNA transfer in Vitreoscilla which opens the way for the application of recombinant-DNA techniques to study this unique group of organisms.
Navani
N K
NK
Institute of Microbial Technology, Chandigarh, India.
Joshi
M A
MA
Dikshit
K L
KL
D018274:Electroporation; D020563:Gammaproteobacteria / Q000235:genetics*; D014170:Transformation, Genetic