Expression of genes involved in iron metabolism in mouse intestine.
Intestinal nonheme iron levels and mRNA levels of genes implicated in iron metabolism were measured in mice with altered iron metabolism [chronic (4 wk) and acute (4 days) dietary iron deficiency; iron overload and hypoxia] to investigate their role in the process and regulation of intestinal iron absorption. Mucosal nonheme iron levels were decreased by both chronic and acute iron deficiency and increased by iron overload but were not affected by hypoxia. There was evidence of a gradient of mucosal nonheme iron along the small intestine (duodenum, jejunum > ileum). There were also regional differences in H-ferritin (duodenum > ileum) and transferrin receptor (ileum > duodenum) mRNA levels. Iron overload produced a decrease in transferrin receptor (TfR) mRNA in the duodenum, with ferritin mRNA levels unaffected in both the duodenum and ileum. Chronic iron deficiency induced a twofold increase in TfR mRNA levels in both the duodenum and ileum, whereas H- and L-ferritin mRNA levels did not change significantly. The ratio of H- to L-ferritin mRNA decreased significantly during exposure to hypoxia; however, individual ferritin and TfR mRNA levels were not significantly altered. Calreticulin (mobilferrin), cysteine-rich intestinal protein, and H(+)-adenosinetriphosphatase mRNA levels were virtually unchanged in all models. A comparison with previously published data on changes in iron absorption leads us to conclude that 1) iron absorption can be altered independently of effects on transcripts of genes for iron-related proteins, and 2) it is not essential for iron absorption to be coordinated with regulation of mucosal iron metabolism.
McKie
A T
AT
Department of Molecular Medicine, King's College School of Medicine and Dentistry, Rayne Institute, London, United Kingdom.
Raja
K B
KB
Peters
T J
TJ
Farzaneh
F
F
Simpson
R J
RJ
D000818:Animals; D015342:DNA Probes; D004386:Duodenum / Q000502:physiology; D005293:Ferritins / Q000096:biosynthesis*; D006454:Hemoglobins / Q000378:metabolism; D000860:Hypoxia; D007082:Ileum / Q000502:physiology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology*; D000090463:Iron Deficiencies; D007583:Jejunum / Q000502:physiology; D008099:Liver / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011990:Receptors, Transferrin / Q000096:biosynthesis*; D012016:Reference Values; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D006454:Hemoglobins; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D005293:Ferritins; D007501:Iron