Induction of cyclooxygenase-2 mRNA in gerbil hippocampal neurons after transient forebrain ischemia.
We examined the effect of brain ischemia on neuronal expression of cyclooxygenase-2 gene in the hippocampus. Transient forebrain ischemia was produced by occluding bilateral carotid arteries for 5 min in Mongolian gerbil. Northern blotting and in situ hybridization demonstrated that expression of cyclooxygenase-2 mRNA was transiently induced in the hippocampal neurons. Although future studies will be needed to clarify if induced cyclooxygenase-2 following ischemia is involved in neuronal damage or neuronal protection, selective cyclooxygenase-2 inhibitors may be a new therapeutical approach for the treatment of stroke.
Ohtsuki
T
T
First Department of Medicine, Osaka University Medical School, Japan.
Kitagawa
K
K
Yamagata
K
K
Mandai
K
K
Mabuchi
T
T
Matsushita
K
K
Yanagihara
T
T
Matsumoto
M
M
D000818:Animals; D051546:Cyclooxygenase 2; D004790:Enzyme Induction; D005849:Gerbillinae; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D009474:Neurons / Q000201:enzymology*; D016548:Prosencephalon; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases