The use of SCID mice in biotechnology and as a model for human disease.
The use of SCID (severe combined immunodeficient) mice in medical research and biotechnology has increased tremendously in recent years. This review outlines the major characteristics of these animals and the impediments that they pose to the engraftment of human cells and tissues. The development of our SCID mice pretreatment protocol (anti-asialo GM1 antisera and radiation) is described, and the results of xenotransplantation studies of human cells and tissues in these pretreated animals are outlined. Wherever possible, data from transplantation studies (of human tissues and cells) in pretreated and nonpretreated animals are compared. The potential of our pretreated SCID mice for medical research and biotechnology is discussed.
Sandhu
J S
JS
Department of Surgery, University of Toronto, Ontario, Canada.
Boynton
E
E
Gorczynski
R
R
Hozumi
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001709:Biotechnology; D004195:Disease Models, Animal; D015316:Genetic Therapy; D006086:Graft vs Host Disease / Q000276:immunology; D015658:HIV Infections / Q000628:therapy; D006801:Humans; D017710:Lymphocyte Transfusion; D051379:Mice; D016513:Mice, SCID; D016511:Severe Combined Immunodeficiency / Q000276:immunology; D014181:Transplantation Immunology; D014183:Transplantation, Heterologous; D014614:Vaccines, Synthetic / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D014614:Vaccines, Synthetic