title: The porcine TTR locus maps to chromosome 6q.
abstract: The sequence of a cDNA clone encoding porcine transthyretin (prealbumin) was used to develop polymorphic markers for the TTR locus. The single-strand conformation polymorphism (SSCP) detected is caused by a silent A/T mutation in the penultimate coding codon and can also be revealed as a SacI restriction fragment length polymorphism (RFLP). The TTR locus was mapped to chromosome 6q by segregation and linkage analysis with these polymorphisms. This assignment confirms the predictions of homology between human chromosome 18 and pig chromosome 6q2.5-q2.6.
authors.affiliation: Roslin Institute (Edinburgh), Midlothian, UK.
authors.forename: A L
authors.initials: AL
authors.lastname: Archibald
authors.forename: S
authors.initials: S
authors.lastname: Couperwhite
authors.forename: Z H
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D003001:Cloning, Molecular; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011228:Prealbumin / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D012756:Sheep; D013552:Swine / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011228:Prealbumin; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific