title: Molecular analysis of UFE1, a Saccharomyces cerevisiae gene essential for spore formation and vegetative growth.
abstract: The UFE1 gene of Saccharomyces cerevisiae was cloned, sequenced and characterized. The coding region of UFE1 is separated from the TMP1 gene on chromosome XV by 624 bp. Gene-disruption experiments demonstrated that UFE1 is essential for both the germination of ascospores and for vegetative growth. Translation of the UFE1 coding region generates a protein with significant similarity to cytokeratin and to the coiled-coil region of SED5, USO1 and restin, suggesting that it is involved in the secretory pathway and may also be related to intermediate filament-associated proteins.
authors.affiliation: Department of Biology, Concordia University, 1455 De Maisonaeuve Blvd, W., Montreal H3G 1MB, Canada.
authors.forename: T A
authors.initials: TA
authors.lastname: Downing
authors.forename: R K
authors.initials: RK
authors.lastname: Storms
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018387:Codon, Initiator; D004351:Drug Resistance / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D007381:Intermediate Filament Proteins / Q000235:genetics; D007633:Keratins / Q000235:genetics; D007763:Lac Operon; D008565:Membrane Proteins / Q000235:genetics; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D016366:Open Reading Frames; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D050765:Qa-SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D013940:Thymidylate Synthase / Q000235:genetics; D014158:Transcription, Genetic; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D018387:Codon, Initiator; D005656:Fungal Proteins; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D050765:Qa-SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; C077554:Sed5 protein, S cerevisiae; C103868:UFE1 protein, S cerevisiae; C086888:USO1 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C076744:cytoplasmic linker protein 170; D007633:Keratins; D013940:Thymidylate Synthase