title: Detection and immunolocalization of macrophage migration inhibitory factor in rat iris and ciliary epithelium.
abstract: To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithelial cells of the iris. Western blot analysis of these tissues showed a single band specific for MIF protein. The expression of MIF mRNA in these tissues was further confirmed by reverse transcription-polymerase chain reaction. Since MIF is known to be a potent proinflammatory cytokine, identification of the protein in iris and ciliary epithelial cells suggests the possibility that it may play an important role in ocular inflammation.
authors.affiliation: Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. akimatsu@med.hokudai.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Kotake
authors.forename: Y
authors.initials: Y
authors.lastname: Tagawa
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002924:Ciliary Body / Q000276:immunology*; D004848:Epithelium; D007124:Immunoenzyme Techniques; D007498:Iris / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
chemical_list: D008263:Macrophage Migration-Inhibitory Factors