Quantitative determination of imidazenil, a novel imidazobenzodiazpine carboxamide derivative, by normal phase high-performance liquid chromatography.
Imidazenil, an imidazobenzodiazepine carboxamide derivative, is a novel anxiolytic and anti-convulsant agent recently characterized as a partial allosteric modulator of GABAA receptors. Owing to the pharmacological and pharmacokinetic importance of plasma-level determination, a HPLC method has been developed. Imidazenil was extracted from a plasma sample after a partition with diethyl ether, using alprazolam as internal standard. The analysis was performed by a normal-phase HPLC method with UV detection at 255 nm. The limit of quantitation was 6 ng corresponding to 30 ng/ml of plasma concentration. This procedure has been successfully applied to the quantitation of imidazenil plasma levels in primarily pharmacokinetic studies after a single i.v. and an oral administration of the compound to the rat.
Mariot
R
R
Department of Pharmacokinetics, Fidia Research Laboratories, Padova, Italy.
Zangirolami
L
L
D000284:Administration, Oral; D000818:Animals; D001569:Benzodiazepines / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D018757:GABA Modulators / Q000097:blood* / Q000493:pharmacokinetics; D007093:Imidazoles / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D051381:Rats; D012015:Reference Standards; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D018757:GABA Modulators; D007093:Imidazoles; D007202:Indicators and Reagents; D012997:Solvents; D001569:Benzodiazepines; C082661:imidazenil