title: UVB irradiation upregulation of the Drosophila 1731 retrotransposon LTR requires the same short sequence of U3 region in a human epithelial cell line as in Drosophila cells.
abstract: Phylogenetic analysis of the retrotransposon and retrovirus suggests an evolutionary relationship between them and indicates that transactivation of the long terminal repeat (LTR)-containing retroelements could be ubiquitous. Using constructs expressing a reporter gene under the control of the entire or deleted LTR of 1731, which is a retrotransposable element of Drosophila melanogaster, we were able to show that the UVB-irradiation activation of the 1731-LTR requires the same short sequence of U3 region in a human epithelial cell line as in Schneider's Drosophila cell line (S2). This sequence is similar to the binding sequence of the members of the nuclear factor-kappa B (NF-kappa B)/rel family. In addition, human colonic carcinoma cells (HT29), in response to UVB-irradiation, produce some extracellular factor(s) that activates the 1731-LTR in nonirradiated cells.
authors.affiliation: Institut de Chimie Biologique, Université de Provence, Marseille, France.
authors.forename: E
authors.initials: E
authors.lastname: Faure
authors.forename: M
authors.initials: M
authors.lastname: Best-Belpomme
authors.forename: S
authors.initials: S
authors.lastname: Champion
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects*; D019073:HT29 Cells / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements / Q000235:genetics* / Q000528:radiation effects*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000528:radiation effects*
chemical_list: D018626:Retroelements; D004247:DNA