The activation of K-ras gene at an early stage of lung tumorigenesis in mice.
To clarify the exact timing of K-ras gene mutational activation in lung tumorigenesis of mice, we applied a sensitive mutant allele specific amplification (MASA) method to pulmonary DNA from urethane-treated mice. The activation of K-ras gene with 61st codon AT mutation was detected in the lungs of mice at day 14 but not day 7 after urethane treatment by MASA. The mutation of MASA products was also checked by XbaI restriction fragment length polymorphism analysis and DNA sequencing. These data suggest that the mutation of K-ras gene in the lungs of mice treated with urethane occurred at the early stage of lung tumorigenesis.
Ichikawa
T
T
School of Home Economics, Mukogawa Women's University, Hyogo, Japan.
Yano
Y
Y
Uchida
M
M
Otani
S
S
Hagiwara
K
K
Yano
T
T
D000818:Animals; D002273:Carcinogens; D011905:Genes, ras / Q000235:genetics*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D051379:Mice; D016296:Mutagenesis; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D014520:Urethane
D002273:Carcinogens; D014520:Urethane