An ocular model of Adenovirus type 5 infection in the rabbit.
Adenoviruses are a common cause of viral eye disease in humans. Recently, new antiviral drugs have been developed, which possess promising anti-adenovirus activity. In order to test these drugs, an ocular model of adenovirus infection was developed in 10 Hollander rabbits following topical, intrastromal and subconjunctival inoculation with a standard laboratory serotype (Adenovirus type 5 from The American Type Culture Collection). Clinical signs of infection-conjunctivitis, keratitis, corneal edema, subepithelial opacities, anterior chamber reaction and iritis-were evaluated. Adenovirus was isolated from the eye several days after inoculation and intra-epithelial viral replication was detected using the replica-technique. Reproducible ocular infection was clinically demonstrated in all rabbits.
De Hauwere
B
B
Dienst Oogheelkunde, U.Z. Leuven.
Lutz
A
A
Maudgal
P C
PC
Neyts
J
J
de Clercq
E
E
D000225:Adenine / Q000031:analogs & derivatives / Q000627:therapeutic use; D000256:Adenoviridae / Q000254:growth & development / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000188:drug therapy / Q000382:microbiology*; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives / Q000627:therapeutic use; D004195:Disease Models, Animal; D005128:Eye Diseases / Q000188:drug therapy / Q000382:microbiology*; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000627:therapeutic use; D011817:Rabbits; D014779:Virus Replication
D000998:Antiviral Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D003596:Cytosine; C050127:9-(S)-(3-hydroxy-2-(phosphonomethoxy)propyl)adenine; D000225:Adenine; D000077404:Cidofovir