An improved method for the detection of Tospoviruses using the polymerase chain reaction.
A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.
Mumford
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RA
School of Biological Sciences, University of Birmingham, UK.
Barker
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I
Wood
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KR
D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis*; D017865:Tospovirus / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral