Comparison of ribosomal DNA sites in Lolium species by fluorescence in situ hybridization.
The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of seven Lolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in the L. multiflorum, seven in L. perenne and nine in L. rigidum var. rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. In L. multifiorum and L.perenne the 5S sites were on the short arm of chromosome 3. However, in L. rigidum var. rigidum the 5S rDNA site was found in either of the two positions.
Thomas
H M
HM
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth. huw.thomas@wpbs.ac.uk
Harper
J A
JA
Meredith
M R
MR
Morgan
W G
WG
Thomas
I D
ID
Timms
E
E
King
I P
IP
D002874:Chromosome Mapping; D002875:Chromosomes; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005456:Fluorescent Dyes; D017343:Genes, Plant / Q000235:genetics; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D008129:Lolium / Q000235:genetics*; D008856:Microscopy, Fluorescence; D012341:RNA, Ribosomal, 5S / Q000235:genetics
D015342:DNA Probes; D004275:DNA, Ribosomal; D005456:Fluorescent Dyes; D012341:RNA, Ribosomal, 5S