Thymosin beta 15: a novel regulator of tumor cell motility upregulated in metastatic prostate cancer.
The Dunning rat prostatic carcinoma is a model system where cell motility closely correlates with the metastatic phenotype. We have identified a novel gene, upregulated in the highly motile and metastatic Dunning cancer cell lines, that represents a new member of the thymosin-beta family, thymosin beta 15. Transfection of antisense thymosin beta 15 constructs into rat prostatic carcinoma cells demonstrates that this molecule positively regulates cell motility, a critical component of the metastatic pathway. Thymosin beta 15 levels are elevated in human prostate cancer and correlate positively with the Gleason tumor grade. Thymosin beta 15 may represent a potential new biochemical marker for human prostate cancer progression.
Bao
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L
Department of Surgery and Cell Biology, Harvard Medical School, Children's Hospital, Boston, Massachusetts 02115, USA.
Loda
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M
Janmey
P A
PA
Stewart
R
R
Anand-Apte
B
B
Zetter
B R
BR
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002465:Cell Movement / Q000502:physiology*; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D006965:Hyperplasia; D008297:Male; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009928:Organ Specificity; D011467:Prostate / Q000737:chemistry / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats; D011993:Recombinant Fusion Proteins; D013947:Thymosin / Q000032:analysis / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000199:Actins; D014408:Biomarkers, Tumor; D016372:RNA, Antisense; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011993:Recombinant Fusion Proteins; C525161:thymosin beta15, rat; D013947:Thymosin