Isolation and characterization of a novel secretory protein, stromal cell-derived factor-2 (SDF-2) using the signal sequence trap method.
With use of the signal sequence trap method, we isolated a cDNA encoding a novel secretory protein, SDF-2, from the mouse stromal cell line, ST2. The human homologue of SDF-2 was also isolated. The amino acid (aa) sequences deduced from both the clones were conserved more than 92%. The chromosomal localization of the human SDF-2 gene was mapped to 17q11.2. The aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-D-mannose:protein mannosyltransferases, Pmt1p [Strahl-Bolsinger et al. (1993) Proc. Natl. Acad. Sci. USA 90, 8164-8168] and Pmt2p [Lussier et al. (1995) J. Biol. Chem. 270, 2770-2775], whose activities have not been detected in higher eukaryotes.
Hamada
T
T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Tashiro
K
K
Tada
H
H
Inazawa
J
J
Shirozu
M
M
Shibahara
K
K
Nakamura
T
T
Martina
N
N
Nakano
T
T
Honjo
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D017124:Conserved Sequence; D018076:DNA, Complementary; D019143:Evolution, Molecular; D015870:Gene Expression; D006801:Humans; D008364:Mannosyltransferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C102467:SDF2 protein, human; C484454:Sdf2 protein, mouse; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase