The expression of types X and VI collagen and fibrillin in rat mandibular condylar cartilage. Response to mastication forces.
Types X and VI collagen and fibrillin were localized by in situ hybridization and immunohistochemical methods in the mandibular condyles of rats, and the response of these molecules to post-weaning diets of soft food, ordinary pellets, or hardened pellets was studied. Type X collagen was synthesized, particularly in conditions of soft food consistency, by cells in the perichondrium-periosteum and in the bone and by cells at the erosion front between cartilage and bone. Type X collagen synthesis diminished under higher compression forces due to mastication and with increasing age. Type VI collagen and fibrillin were synthesized by cells in the perichondrium-periosteum and by chondrocytes and by stromal osteoblasts and were not modified by higher mechanical forces. In contrast to previous findings in the growth plate of long bones, type X collagen in the mandibular condyle was not synthesized by hypertrophic chondrocytes but was associated with cells of the osteoblastic rather than the chondroblastic phenotype.
Salo
L A
LA
Institute of Dentistry, University of Oulu, Finland.
Hoyland
J
J
Ayad
S
S
Kielty
C M
CM
Freemont
A
A
Pirttiniemi
P
P
Kantomaa
T
T
Grant
M E
ME
Thomas
J T
JT
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D000071837:Fibrillins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008335:Mandibular Condyle / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008409:Mastication / Q000502:physiology; D008840:Microfilament Proteins / Q000096:biosynthesis*; D051381:Rats
D000071837:Fibrillins; D008840:Microfilament Proteins; D003094:Collagen