title: Isolation and characterization of two cDNAs from Atlantic cod encoding two distinct psychrophilic elastases.
abstract: The cDNAs encoding two different Atlantic cod elastases have been isolated and sequenced. The predicted amino acid sequences revealed two preproelastases, consisting of a signal peptide, an activation peptide and a mature enzyme of 242 and 239 amino acids. Amino acid sequence identity between the two cod elastases was 60.1% and identity with mammalian elastases ranged from 50-64%. The two cod elastases contain all the major structural features common to serine proteases, such as the catalytic triad His57, Asp102 and Ser195. Both cod elastases have a high content of methionine, consistent with previous findings in psychrophilic fish enzymes.
authors.affiliation: Science Institute, University of Iceland, Dunhaga, Reykjavik, Iceland.
authors.forename: E
authors.initials: E
authors.lastname: Gudmundsdóttir
authors.forename: R
authors.initials: R
authors.lastname: Spilliaert
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.forename: C S
authors.initials: CS
authors.lastname: Craik
authors.forename: J B
authors.initials: JB
authors.lastname: Bjarnason
authors.forename: A
authors.initials: A
authors.lastname: Gudmundsdóttir
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D005399:Fishes / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D021382:Protein Sorting Signals; D010196:Pancreatic Elastase