A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection.
Progenitor cell failure in the erythroid lineage is a particular problem in bone marrow failure. To provide insight into early erythopoietic development we used sensitive techniques to examine the effects of SCF, IL-3 and MIP-1 alpha on two developmentally arrested progenitor cell lines, HEL and K562. Quantitative flowcytometric analysis showed that both expressed receptors (SCF > MIP-1 alpha > IL-3). Qualitative analysis revealed HEL cells expressed more receptors than K562 cells. Clonogenic assays with sensitive haemoglobin detection showed that SCF and IL-3 did not support HEL development and reduced haemoglobin production. MIP-1 alpha reduced partially developed HEL colonies and haemoglobin in developed colonies. SCF increased development, but not haemoglobin in K562 cells, with IL-3 being more effective in both. MIP-1 alpha increased the proportion of well-developed K562 colonies but not haemoglobin. This suggests SCF, IL-3 and MIP-1 alpha all have a role to play in early erythroid cellular development, with differing actions depending on the stage of development.
McGuckin
C P
CP
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, U.K.
Liu
W M
WM
Gordon-Smith
E C
EC
Uhr
M R
MR
D002454:Cell Differentiation; D002460:Cell Line; D054407:Chemokine CCL4; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005434:Flow Cytometry; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology*; D018121:Receptors, Cytokine / Q000378:metabolism*; D019089:Stem Cell Factor / Q000494:pharmacology*
D054407:Chemokine CCL4; D006454:Hemoglobins; D007377:Interleukin-3; D019402:Macrophage Inflammatory Proteins; D018121:Receptors, Cytokine; D019089:Stem Cell Factor