title: Determination of patulin in apple juice by liquid chromatography: collaborative study.
abstract: An AOAC International-International Union of Pure and Applied Chemistry-International Fruit Juice Union (AOAC-IUPAC-IFJU) collaborative study was conducted to evaluate a liquid chromatographic (LC) procedure for determination of patulin in apple juice. Patulin is a mold metabolite found naturally in rotting apples. Patulin is extracted with ethyl acetate, treated with sodium carbonate solution, and determined by reversed-phase LC with UV detection at 254 or 276 nm. Water, water-tetrahydrofuran, or water-acetonitrile was used as mobile phase. Levels determined in spiked test samples were 20, 50, 100, and 200 micrograms/L. A test sample naturally contaminated at 31 micrograms/L was also included. Twenty-two collaborators in 10 countries analyzed 12 test samples of apple juice. Recoveries averaged 96%, with a range of 91-108%. Repeatability relative standard deviations (RSDr) ranged from 10.9 to 53.8%. The reproducibility relative standard deviation (RSDR) ranged from 15.1 to 68.8%. The LC method for determination of patulin in apple juice has been adopted first action by AOAC INTERNATIONAL.
authors.affiliation: Analytical Chemical Services of Columbia, Inc., MD 21045, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Brause
authors.forename: M W
authors.initials: MW
authors.lastname: Trucksess
authors.forename: F S
authors.initials: FS
authors.lastname: Thomas
authors.forename: S W
authors.initials: SW
authors.lastname: Page
mesh_terms: D001628:Beverages / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination; D005638:Fruit; D010365:Patulin / Q000032:analysis*
chemical_list: D010365:Patulin