Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene.
Ocular albinism type 1 (OA1) is an X-linked human genetic disorder that affects retinal pigment cells and, to a lesser degree, neural crest-derived melanocytes. The OA1 gene is located close to the pseudoautosomal region and predicts a novel protein whose function is unknown. However, histologic studies of affected patients have suggested a potential role in melanosome biogenesis. Here we report the isolation and characterization of the mouse homolog of the human OA1 gene, termed Moa1. Two Moa1 isoforms were isolated from a melanoma cDNA library and predicted to encode proteins of 405 and 249 amino acids with six and two transmembrane-spanning regions, respectively. Interspecific backcross mapping yielded a map order and distances (cM) of cen-Moa1-3.1 +/- 1.8-Piga-2.1 +/- 1.5-Amel, indicating that Moa1 is located much farther away from the pseudoautosomal region than its human homolog. In adult tissues, both Moa1 isoforms were detected in the eye by Northern hybridization. In neonatal tissues, Moa1 RNA was detected in both skin and eyes by Northern hybridization and was not affected by the absence of pigment in mice carrying the albino mutation, or by the type of pigment synthesized, i.e., eumelanin vs pheomelanin, in mice carrying the black-and-tan mutation. Expression of Moa1 RNA was not detected in embryonic tissues by Northern analysis or by in situ hybridization despite the active synthesis of ocular pigment by E16.5. These results provide insight into the structure and possible function of the OA1 protein and suggest a more complex relationship between the human and mouse X chromosomes than was previously thought to exist.
Newton
J M
JM
Department of Pediatrics, Stanford University School of Medicine, California 94305-5428, USA.
Orlow
S J
SJ
Barsh
G S
GS
D016117:Albinism, Ocular / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D008040:Genetic Linkage; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014960:X Chromosome
D005136:Eye Proteins; C095276:GPR143 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C103527:Moa1 protein, mouse; D012333:RNA, Messenger; D004247:DNA