Para-chloroamphetamine (PCA)-induced ejaculation in aged rats, semen analysis and artificial insemination.
The present study was undertaken to determine sperm motility and counts in semen yielded by para-chloroamphetamine (PCA)-induced ejaculation of aged rats which had lost their reproductive ability, and to attempt artificial insemination with suspensions of spermatozoa obtained in this way. The semen yielded by PCA-induced ejaculation from aged (75-week-old) rats had average sperm counts of 0.82 +/- 0.69 x 10(7), which were much lower than the average counts (9.42 +/- 1.65 x 10(7)) for semen spontaneously ejaculated by young adult rats (14 weeks old). However, 77.5% of the spermatozoa contained in the PCA-induced semen were rated as showing the most active movement. Spermatozoa collected in this way were injected into the upper parts of both uterine horns or into both ovarian bursae. Both methods made the females pregnant, but the results were better after injection into the ovarian bursae. The offspring born to these females showed no abnormalities.
Saito
T R
TR
Department of Laboratory Animal Science, Nippon Veterinary and Animal Science University, Tokyo, Japan.
Hokao
R
R
Wakafuji
Y
Y
Igarashi
N
N
Agematsu
Y
Y
Takahashi
K W
KW
D000375:Aging; D000818:Animals; D004542:Ejaculation / Q000187:drug effects*; D005260:Female; D007315:Insemination, Artificial; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012661:Semen / Q000166:cytology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology; D010133:p-Chloroamphetamine / Q000494:pharmacology*
D010133:p-Chloroamphetamine