A monoclonal antibody-based enzyme immunoassay for detecting parasite antigenaemia and antigenuria in Schistosomiasis mansoni.
Both specific polycolonal antibody and monoclonal antibody against the microsomal antigen of adult S. mansoni were used to detect antigenaemia and antigenuria by antigen-capture sandwich ELISA in the sera and urine of patients infected with S. mansoni and other parasites. Antigenaemia was detected in 22 sera out of 100 of patients infected with S. mansoni but no antigenuria was detected. None of the sera of S. mansoni free patients were positive for microsomal antigen. More standardization of the technique and more refining of the reagents used is required to improve the sensitivity of the test.
Kamel
M A
MA
Department of Clinical Pathology, Faculty of Medicine, Cairo University, Egypt.
Kamal
K A
KA
Mansour
M
M
el Adawi
A I
AI
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007106:Immune Sera; D007075:Immunoglobulin M