title: Nucleic acid sequence and affiliation of pLUG10, a novel cadmium resistance plasmid from Staphylococcus lugdunensis.
abstract: Tolerance of Staphylococcus lugdunensis to relatively high levels of cadmium is mediated by a 3117-bp plasmid, pLUG10. Sequencing reveals three major open reading frames (ORFs) in a single orientation. One ORF encompasses the origin of replication and its predicted product (RepL: 350 amino acids (aa)) shows 70% homology in its deduced aa sequence with Rep proteins of the pT181 family. A lagging strand conversion signal (palA) very similar to that of class 1 plasmids is present outside the rep-ori locus. The other two ORFs of 209 and 116 aa show 92.5% homology between their deduced aa sequences and the CadB and CadX peptides from the pOX6 plasmid of Staphylococcus aureus. The CadX-like peptide is 40% homologous to the S. aureus CadC product. Deletion of the C-terminal cadX gene by restriction enzyme digestion or frame-shift inactivation of the cadB gene reduced, but did not completely abolish, cadmium resistance. The two gene products may act cooperatively to confer cadmium resistance in S. lugdunensis.
authors.affiliation: Département de Recherche en Bactériologie Médicale (UPRES 1655), Faculté de Médecine R. Laennec, Lyon, France.
authors.forename: L B
authors.initials: LB
authors.lastname: Chaouni
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: F
authors.initials: F
authors.lastname: Vandenesch
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics* / Q000502:physiology; D011815:R Factors / Q000235:genetics / Q000302:isolation & purification; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002104:Cadmium