[Immunoglobulin heavy chain variable region(VH) genes in B cell clones producing anti-colon antibodies in ulcerative colitis].
We have established anti-colon antibodies producing B cell clones from the patient with ulcerative colitis, and analyzed the immunoglobulin heavy chain variable region (VH) gene segments of these clones using RT-PCR with VH family specific primers. VH3 family was used by all ten clones from ulcerative colitis that produced anti-colon antibodies, while various VH gene families were used by eight clones from normal controls. By nucleotide sequence analysis, two LPL clones were thought to be derived from a germ line gene segment, VH26. However, other three PBL clones were derived from 22-2B, 9-1 and 1.9-III, respectively. These results suggest the heterogeneity of the colonic antigens that autoantibodies in ulcerative colitis recognize.
Inoue
N
N
Internal Medicine, Ichikawa General Hospital.
Hisamatsu
T
T
Iwao
Y
Y
Watanabe
M
M
Ishii
H
H
Mizuno
Y
Y
Hibi
T
T
D000921:Antibody-Producing Cells; D001323:Autoantibodies; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000276:immunology; D003106:Colon / Q000276:immunology; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006801:Humans
D001323:Autoantibodies