IL-2 effects on allogeneic and autologous transplant haemopoietic progenitors in long-term cultures.
IL-2 therapy may be useful in situations with a low tumour burden, such as after autologous transplantation. However, conflicting reports about the deleterious effects of this cytokine on haemopoiesis have precluded its widespread use. To study IL-2 effects on haemopoietic transplant progenitors we established long-term cultures (Dexter-type) with cells from allogeneic marrow and marrow/peripheral blood cell infusates of autologous transplants with different concentrations of IL-2 (0-1000 IU/ml). Percentage of CD56+ cells was also determined in cultures. IL-2 induced an inhibitory effect on stroma and an increase in the percentage of CD56+ cells compared with controls. No deleterious effect either in the production of BFU-E or CFU-GM weekly or over the whole period of culture was observed. Our results suggest that IL-2 is able to induce an increase in CD56+ cells early after transplantation without a deleterious effect on long-term haemopoiesis.
López-Jiménez
J
J
Department of Haematology, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, Spain.
Quiroga
R
R
Ramos
P
P
Cerveró
C
C
Parra
C
C
García-Laraña
J
J
Pérez-Oteyza
J
J
Roldán
E
E
Heindrichs
B
B
Odriozola
J
J
D001853:Bone Marrow / Q000473:pathology*; D016026:Bone Marrow Transplantation; D019002:CD56 Antigen / Q000032:analysis; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D019002:CD56 Antigen; D007376:Interleukin-2