Adhesion molecules and IL-1 costimulate T lymphocytes in the autologous MECLR in psoriasis.
Membrane molecules such as CD36 (OKM5), intercellular adhesion molecule-1 (ICAM-1, CD54), gamma interferon-induced protein 10 (gamma-IP10) and IL-1 are induced and/or upregulated in psoriatic epidermis. These molecules have important accessory, trafficking or signalling functions in the immune system and also play a role in the pathophysiology of psoriasis. The relevance of adhesion molecules, CD36 and epidermal IL-1 in psoriasis was studied in vitro in the autologous mixed epidermal cell - T lymphocyte reaction (MECLR). Their level of expression was quantitated in epidermal cell suspensions (ECS) from patients with psoriasis and their function was assessed by blocking with specific mAbs and antisera or by depleting CD36+ cells from the ECS prior to the MECLR. ECS from psoriatic lesions contained increased numbers of CD36+ (23 +/- 12%), ICAM-1(+) (31 +/- 14%) and IL-1(+) (57 +/- 21%) cells. The autologous MECLR was inhibited in samples from all patients by mAb to CD2 (LFA-2), CD11a (LFA-1alpha), CD18 (LFA-1beta), ICAM-1, CD58 (LFA-3) and an antiserum to IL-1beta. Thus, adhesion molecules facilitate inflammation in psoriasis not only via adhesion and recruitment of T lymphocyte in psoriatic lesions, but also via activation of T cells. Furthermore CD36 molecules on psoriatic epidermal cells do not costimulate autologous T lymphocytes in psoriasis. The observed costimulatory function of IL-1beta in the MECLR emphasizes its relevance in psoriasis.
Prens
E
E
Department of Immunology, Erasmus University Rotterdam, The Netherlands.
t Hooft-Benne
K
K
Tank
B
B
Van Damme
J
J
van Joost
T
T
Benner
R
R
D000911:Antibodies, Monoclonal; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D000078404:Epidermal Cells; D006801:Humans; D007106:Immune Sera; D007108:Immune Tolerance; D016130:Immunophenotyping; D007375:Interleukin-1 / Q000502:physiology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D011565:Psoriasis / Q000503:physiopathology*; D017463:Receptors, Complement 3b / Q000097:blood; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000502:physiology*; D014182:Transplantation, Autologous
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007106:Immune Sera; D007375:Interleukin-1; D017463:Receptors, Complement 3b