Demonstration of a bilateral projection from the rostral nucleus of the solitary tract to the medial parabrachial nucleus in rat.
This study examined the projection from the rostral nucleus of the solitary tract (rNST) to the medial parabrachial nucleus (mPBN) in male Wistar rats using DiI as a retrograde tracer and biotinylated dextran as an anterograde tracer. Following successful unilateral injection of DiI into the mPBN (n = 8), retrogradely labeled neurons were always found in the rNST both ipsilateral and contralateral to the pontine injection site. Significantly, approximately 25% of the total number of DiI-labeled neurons were located in the contralateral rNST. The labeled neurons were located throughout the rostral-caudal extent of the rNST with the most cells being located in the central portion of the nucleus, and the fewest located ventromedially and dorsolaterally. Supporting the findings of the retrograde labeling study, axons and terminals, anterogradely labeled by injecting biotinylated dextran unilaterally into the rNST (n = 4), were always found in both the ipsilateral and contralateral mPBN. Although the intensity of anterograde labeling was higher ipsilaterally, a mirror-image staining pattern consistently was present contralaterally. These results indicate that there is a substantial contralateral component of the projection from the rNST to the mPBN. This suggests that convergence of gustatory information from the two sides of the oral cavity may occur within the pons before processing in higher brain centers. These findings may have important implications as to where and how bilateral gustatory information is processed and integrated.
Williams
J B
JB
Biology Department, Stetson University, DeLand, FL 32720, USA.
Murphy
D M
DM
Reynolds
K E
KE
Welch
S J
SJ
King
M S
MS
D000818:Animals; D001369:Axons; D001710:Biotin; D002232:Carbocyanines; D003911:Dextrans; D005456:Fluorescent Dyes; D008297:Male; D009434:Neural Pathways; D009474:Neurons / Q000648:ultrastructure; D011149:Pons / Q000166:cytology*; D017729:Presynaptic Terminals; D051381:Rats; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000166:cytology*; D013649:Taste / Q000502:physiology
C101089:CM-DiI; D002232:Carbocyanines; D003911:Dextrans; D005456:Fluorescent Dyes; D001710:Biotin