Cell surface alterations by taxol associated with abnormal morphogenesis in the chick embryo.
The anticancer drug taxol brings about its biological effects by altering the stability of microtubules. We have examined the effects of taxol on early morphogenesis in chick embryos cultured in vitro. Taxol induced various abnormalities in the developing nervous system, heart and somites as well as general retardation of development. SEM studies revealed that taxol treatment leads to dramatic alterations in the embryonic cell surfaces. Time-course experiments demonstrated that the action of taxol is very rapid and becomes evident within a few minutes at the ultrastructural level. Taxol thus throws embryonic cell adhesion and motility out of balance. This appears to be the major cause of abnormal morphogenesis in taxol-treated embryos.
Patwardhan
V
V
Division of Animal Science, Agharkar Research Institute, Pune, India.
Ghate
H V
HV
Ghaskadbi
S
S
D000818:Animals; D002448:Cell Adhesion; D002642:Chick Embryo / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D009024:Morphogenesis / Q000187:drug effects; D017239:Paclitaxel / Q000633:toxicity*
D017239:Paclitaxel