[Gene K-ras mutation in cells of endometrial adenocarcinoma in women].
A simple, sensitive method of DNA analysis of nucleotide substitutions, namely, single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP analysis) was used for detection of mutated K-ras gene in specimens of human endometrial cancer. The mobility shifts of single-strand DNA fragment was seen in 3 of 8 analysed neoplasms: two in stage I and one in stage II, according to FIGO classification, K-ras gene mutation was found in 3 out of 4 patients over 66 years old.
Semczuk
A
A
II Kliniki Ginekologii Operacyjnej Akademii Medycznej w Lublinie.
Wojcierowski
J
J
Kitlińska
J
J
Kotarski
J
J
Rózańska
K
K
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational