title: Chromatographic fractionation and analysis by mass spectrometry of conjugated metabolites of bis(2-ethylhexyl)phthalate in urine.
abstract: Mono(2-ethylhexyl)phthalate (MEHP), the primary metabolite of the plasticizer bis(2-ethylhexyl)phthalate (DEHP), was given to guinea pigs and mice and the methods for the isolation, separation and analysis of its metabolites in urine were developed. Following solid-phase extraction with octadecylsilane-bonded silica, individual metabolites were purified and separated using a combination of ion-exchange chromatography on lipophilic gels and reversed-phase high-performance liquid chromatography. Analysis of intact conjugates, as well as nonconjugated metabolites, was performed by fast atom bombardment mass spectrometry (FAB-MS) and, after derivatization, by gas chromatography-mass spectrometry. Enzymatic methods were used for further characterization. The study confirms glucuronidation as the major conjugation pathway for MEHP in the investigated species. Although less important quantitatively, glucosidation is shown to be an alternative conjugation pathway in mice. The methods developed were applied to a sample of urine from a hyperbilirubinemic newborn infant subjected to DEHP-exposure in conjunction with an exchange transfusion. It was demonstrated that metabolites of DEHP were excreted in amounts which could be analyzed by FAB-MS.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Egestad
authors.forename: G
authors.initials: G
authors.lastname: Green
authors.forename: P
authors.initials: P
authors.lastname: Sjöberg
authors.forename: E
authors.initials: E
authors.lastname: Klasson-Wehler
authors.forename: J
authors.initials: J
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004051:Diethylhexyl Phthalate / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005960:Glucosides / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D006932:Hyperbilirubinemia / Q000652:urine; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005960:Glucosides; D005965:Glucuronates; D004051:Diethylhexyl Phthalate