title: Flow cytometric analysis of granulosa cells from follicular fluid after follicular stimulation.
abstract: Granulosa cells are not easily accessible, unless they are examined in follicular fluid after oocyte retrieval. These samples are usually contaminated with blood. We have set up a general technique for analysis of granulosa cells without physically separating them from blood cells. The sample is stained with CD45, which is a pan-leukocyte marker, and granulosa cells are consecutively selected as CD45 negative during flow cytometric analysis. Analysis of forward scatter of the granulosa cells, which is correlated to cell size, shows a wide size range throughout the whole population rather than two distinct populations as previously suggested.
authors.affiliation: NURTURE (Nottingham University) Department of Obstetrics and Gynaecology, University Hospital, Queen's Medical Centre, Nottingham, UK.
authors.forename: D D
authors.initials: DD
authors.lastname: Neubourg
authors.forename: A
authors.initials: A
authors.lastname: Robins
authors.forename: S
authors.initials: S
authors.lastname: Fishel
authors.forename: L
authors.initials: L
authors.lastname: Gibbon
mesh_terms: D005260:Female; D005434:Flow Cytometry; D015571:Follicular Fluid / Q000166:cytology*; D006107:Granulosa Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D008596:Menotropins / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects*
chemical_list: D008596:Menotropins; D017493:Leukocyte Common Antigens