Serum levels of 3-deoxyglucosone and tissue contents of advanced glycation end products are increased in streptozotocin-induced diabetic rats with nephropathy.
To investigate a role of the Maillard reaction in the pathogenesis of diabetic nephropathy, we measured serum levels of 3-deoxyglucosone (3-DG), a potent protein cross-linking intermediate of the Maillard reaction, and tissue contents of advanced glycation end products (AGEs) in streptozotocin (STZ)-induced diabetic rats. We quantified serum 3-DG using gas chromatography/mass spectrometry, and measured AGE contents in tissues using a competitive enzyme-linked immunosorbent assay with a monoclonal anti-AGE antibody. The STZ-induced diabetic rats showed nephropathy with proteinuria, hypoproteinemia, hyperlipidemia and reduced creatinine clearance. Serum levels of 3-DG in the STZ-induced diabetic rats (mean +/- 3.46 +/- 0.23 mumol/l) were significantly (p < 0.01) higher than those in control rats (1.23 +/- 0.13 mumol/l). AGE contents in the kidney and the lens obtained from the STZ-induced diabetic rats (398 +/- 45 and 816 +/- 200 arbitrary units, respectively) were also significantly (p < 0.01) higher than those in the control rats (122 +/- 10 and 299 +/- 50 arbitrary units, respectively). The results indicate that increased levels of serum 3-DG and renal tissue. AGEs may be related to the occurrence of diabetic nephropathy.
Niwa
T
T
Nagoya University Branch Hospital, Japan.
Miyazaki
T
T
Katsuzaki
T
T
Tatemichi
N
N
Takei
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001835:Body Weight / Q000502:physiology; D003404:Creatinine / Q000652:urine; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism* / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000378:metabolism* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D017127:Glycation End Products, Advanced / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008297:Male; D011507:Proteinuria / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000652:urine
D000911:Antibodies, Monoclonal; D017127:Glycation End Products, Advanced; D014508:Urea; D003847:Deoxyglucose; D003404:Creatinine; C016350:3-deoxyglucosone