title: Immunohistochemical analysis of presenilin-1 expression in the mouse brain.
abstract: At least 22 different mutations associated with early-onset familial Alzheimer's disease (AD) in various kindreds have been reported to occur in a recently identified gene on chromosome 14, presenilin 1 (PS-1) (Sherrington et al. (1995) Nature 375, 754-760 [1] and reviewed by Van Broeckhoven (1995) Nat. Genet. 11, 230-231 [2]). In order to study the localization of PS-1 in the brain, we raised a polyclonal antiserum specific to a fragment of the predicted protein sequence of PS-1. PS-1 immunostaining was found intracellularly, in the perikaria of discrete cells, mostly neurons, appearing as thick granules, resembling large-size vesicles. These granules were located in the periphery of cell bodies and extended into dendrites and neurites. PS-1 expression was found to be broadly distributed throughout the mouse brain, not only in structures involved in AD pathology, but also in structures unaltered by this disease.
authors.affiliation: Rhone-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, France.
authors.forename: S
authors.initials: S
authors.lastname: Moussaoui
authors.forename: C
authors.initials: C
authors.lastname: Czech
authors.forename: L
authors.initials: L
authors.lastname: Pradier
authors.forename: V
authors.initials: V
authors.lastname: Blanchard
authors.forename: B
authors.initials: B
authors.lastname: Bonici
authors.forename: M
authors.initials: M
authors.lastname: Gohin
authors.forename: A
authors.initials: A
authors.lastname: Imperato
authors.forename: F
authors.initials: F
authors.lastname: Revah
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002883:Chromosomes, Human, Pair 14; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D053764:Presenilin-1; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; C508853:PSEN1 protein, human; D010446:Peptide Fragments; D053764:Presenilin-1; D012333:RNA, Messenger