title: The use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin.
abstract: Elastase, cathepsin G and azurocidin from human neutrophils are key components of body inflammatory defense. Perturbations in regulation of their activities lead to many serious pathological states. The paper describes a simple, fast and efficient method of joint purification of these proteins with the use of sequential affinity chromatography on squash trypsin inhibitor (CMTI I) and bovine pancreatic trypsin inhibitor (BPTI).
authors.affiliation: Institute of Biochemistry, Wrocław University, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Watorek
authors.forename: A
authors.initials: A
authors.lastname: Polanowski
authors.forename: T
authors.initials: T
authors.lastname: Wilusz
mesh_terms: D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000302:isolation & purification*; D002352:Carrier Proteins; D056649:Cathepsin G; D002403:Cathepsins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019272:Leukocyte Elastase / Q000302:isolation & purification*; D009504:Neutrophils / Q000201:enzymology*; D012697:Serine Endopeptidases
chemical_list: C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D019272:Leukocyte Elastase