Bafilomycin A1 induces apoptosis in PC12 cells independently of intracellular pH.
PC12 cells growth-arrested with bafilomycin A1 died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A1-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A1 than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH4Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A1, suggesting that bafilomycin A1-induced apoptosis occurs independently of intracellular pH in PC12 cells.
Kinoshita
K
K
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Waritani
T
T
Noto
M
M
Takizawa
K
K
Minemoto
Y
Y
Nishikawa
A
A
Ohkuma
S
S
Nishikawa
Y
Y
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D016716:PC12 Cells / Q000166:cytology / Q000187:drug effects*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000187:drug effects; D051381:Rats; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002843:Chromatin; D004791:Enzyme Inhibitors; D018942:Macrolides; D011500:Protein Synthesis Inhibitors; D011506:Proteins; D000643:Ammonium Chloride; D001312:Aurintricarboxylic Acid; C040929:bafilomycin A1; D003513:Cycloheximide