title: Structure of N-glycans on the S3- and S6-allele stylar self-incompatibility ribonucleases of Nicotiana alata.
abstract: Self-incompatibility is a mechanism developed by many plants to prevent inbreeding. The products of the self-incompatibility (S)-locus in the styles of solanaceous plants are a series of glycoproteins with ribonuclease activity. In this study, we report on the N-glycans from the stylar self-incompatibility S3- and S6-ribonucleases of Nicotiana alata, which were enzymically released and fractionated by high-pH anion-exchange HPLC. A total of 14 N-glycans were identified and characterized by a combination of electrospray-ionization mass-spectrometry, 1H-NMR spectros-copy, chemical degradation, and methylation analyses. This patterns of N-glycosylation is much more complex than that previously found on the N.alata S1- and S2-RNases, each of which contained only four N-glycans.
authors.affiliation: Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Oxley
authors.forename: S L
authors.initials: SL
authors.lastname: Munro
authors.forename: D J
authors.initials: DJ
authors.lastname: Craik
authors.forename: A
authors.initials: A
authors.lastname: Bacic
mesh_terms: D000483:Alleles; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011134:Polysaccharides / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology*
chemical_list: D011134:Polysaccharides; D012260:Ribonucleases