Binding of extracellular matrix proteins to the surface of anaerobic bacteria.
The binding of fibronectin, vitronectin, collagen and sialoprotein to 65 anaerobic strains was investigated by means of latex agglutination tests. The binding of fibronectin, collagen and lactoferrin to the same strains was also tested by means of 125I-labelled proteins. The strains were isolated from abdominal infections (55%), from the faeces of healthy subjects (29%) or from the depths of tonsils removed at tonsillectomy (16%). The binding of fibronectin and collagen to Bacteroides fragilis strains, tested by the latex agglutination assay, was stronger than their binding to other species. The vitronectin binding of the strains was less common, but was always accompanied by fibronectin binding. Binding to fibronectin-coated beads was inhibited by pre-incubation of the bacterial cells with soluble fibronectin and by heat or protease treatment of the bacterial suspension. No inhibition of the binding was observed with carbohydrates. None of the 65 strains exhibited any binding to fetuin or asialofetuin; 8% of the strains had a binding site for mucin. The binding to mucin-coated beads was inhibited by pre-incubation of the cells with mucin. The radiolabelling method indicated a low binding to 125I-fibronectin. The binding of 125I-collagen-I and 125I-lactoferrin was higher for the anaerobic strains tested.
Szöke
I
I
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Pascu
C
C
Nagy
E
E
Ljung
A
A
Wadström
T
T
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001439:Bacteroides / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007841:Latex Fixation Tests; D012795:Sialoglycoproteins / Q000378:metabolism; D019096:Vitronectin / Q000378:metabolism
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007457:Iodine Radioisotopes; D012795:Sialoglycoproteins; D019096:Vitronectin; D003094:Collagen