title: Vaccination with alum-precipitated recombinant Ancylostoma-secreted protein 1 protects mice against challenge infections with infective hookworm (Ancylostoma caninum) larvae.
abstract: Ancylostoma-secreted protein 1 (ASP-1) is the major protein secreted by infective hookworm larvae (Ancylostoma caninum). The Escherichia coli-expressed recombinant protein was evaluated as a vaccine antigen in a mouse model of ancylostomiasis. A. caninum larvae migrate through mouse lungs, with maximal migration occurring 48-54 h after oral infection. Quantitative larval recovery from the lungs at this time was used as an end point for vaccine evaluation. All mice developed antibodies to recombinant ASP-1 (rASP-1) after immunization and boosting with the alum-precipitated protein. The immunized mice had their worm burden reduced 79% (P < .0001) compared with controls. Immunization with rASP-1 in the presence of Corynebacterium parvum adjuvant also showed a vaccine effect (63% protection; P < .0001). The possibility that this protective effect resulted from delayed larval lung entry was excluded. rASP-1 offers promise as a hookworm vaccine antigen.
authors.affiliation: Department of Pediatrics (Infectious Diseases Division), Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: K
authors.initials: K
authors.lastname: Ghosh
authors.forename: J
authors.initials: J
authors.lastname: Hawdon
authors.forename: P
authors.initials: P
authors.lastname: Hotez
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000724:Ancylostomiasis / Q000517:prevention & control*; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D007814:Larva / Q000276:immunology; D008168:Lung / Q000469:parasitology; D008297:Male; D051379:Mice; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D014614:Vaccines, Synthetic