title: Identification of four complete type 4 pilin genes in a single Kingella denitrificans genome.
abstract: We have cloned and sequenced four complete type 4 pilin genes from the type strain (ATCC 33394) of Kingella denitrificans. Two of these pilin genes, kdpB and kdpD, are in tandem, oriented in the same direction, and encode pilins of only 50% amino acid identity. The kdpA and kdpC loci are separately located from the kdpB-kdpD locus and from each other. At the DNA level kdpA and kdpC are nearly identical to kdpB and encode pilin proteins that are identical to KdpB. Bands of multiple hybridization previously hypothesized to be due to partial silent pilin gene loci are now shown to be due to the presence of 18-bp repeat sequences (IR18) associated with the pilin gene coding regions. These IR18 sequences exist most often as inverted repeats separated by 8 bp. IR18 sequences are structurally similar to the repetitive extragenic palindromic sequences of Escherichia coli, although they have different DNA sequences. The IR18 sequences also demonstrate homology to the DNA uptake sequences of Neisseria gonorrhoeae and may serve a similar function for K. denitrificans.
authors.affiliation: Department of Epidemiology, University of Michigan, Ann Arbor 48109, USA.
authors.forename: S
authors.initials: S
authors.lastname: Weir
authors.forename: L W
authors.initials: LW
authors.lastname: Lee
authors.forename: C F
authors.initials: CF
authors.lastname: Marrs
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D017881:Kingella / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D036781:Fimbriae Proteins