Human alcohol dehydrogenase isoenzyme activity in the sera of non-alcoholic liver cirrhotic patients.
The activities of class I and II alcohol dehydrogenase isoenzymes were examined in the sera of patients with non-alcoholic liver cirrhosis using a fluorometric method. The analysis of these results shows a statistically significant increase (2,5-times) in the activity of class I alcohol dehydrogenase, and no marked differences in the activity of class II in cirrhotic and control patients. The observed increase in total enzyme activity measured using a photometric method was not very high but confirmed the elevation of class I isoenzyme activity. Activities of both classes of alcohol dehydrogenase isoenzymes have a good correlation with aspartate aminotransferase. Class II isoenzyme activity additionally correlates with alkaline phosphatase. These results suggest that serum activity of class I alcohol dehydrogenase is a better indicator of liver cell destruction during non-alcoholic cirrhosis than total enzyme activity, and is comparable with the value of aspartate aminotransferase.
Chrostek
L
L
Department of Biochemical Diagnostics, Medical School, Bialystok, Poland.
Szmitkowski
M
M
D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008103:Liver Cirrhosis / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D005723:gamma-Glutamyltransferase / Q000097:blood
D007527:Isoenzymes; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin