Stability of an inverted repeat in a human fibrosarcoma cell.
Deletions and rearrangements of DNA sequences within the genome of human cells result in mutations associated with human disease. We have developed a selection system involving a neo gene containing a DNA sequence inserted into the NcoI site that can be used to quantitatively assay deletion of this sequence from the chromosome. The spontaneous deletion from the neo gene of a 122 bp inverted repeat occurred at a rate of 2.1 x 10(-8) to <3.1 x 10(-9) revertants/cell/generation in three different cell lines. Deletion of the 122 bp inverted repeat occurred between 6 bp flanking direct repeats. Spontaneous deletion of a 122 bp non-palindromic DNA sequence flanked by direct repeats was not observed, indicating a rate of deletion of <3.1 x 10(-9) revertants/cell/generation. This result demonstrates that a 122 bp inverted repeat can exhibit a low level of instability in some locations in the chromosome of a human cell line.
Kramer
P R
PR
Department of Biochemistry and Biophysics, Texas A&M University, Houston 77030-3303, USA.
Stringer
J R
JR
Sinden
R R
RR
D001483:Base Sequence; D002461:Cell Line, Transformed; D007446:Chromosome Inversion; D004274:DNA, Recombinant; D004351:Drug Resistance / Q000235:genetics; D005354:Fibrosarcoma; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009355:Neomycin / Q000494:pharmacology; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D004274:DNA, Recombinant; D009355:Neomycin