Hypertension does not affect intracellular calcium uptake in human platelets.
The relationship between the Ca2+ transport of platelet endoplasmic reticulum and hypertension was analyzed in 17 untreated patients exhibiting various degrees of hypertension. Each patient underwent a 24-h recording of ambulatory blood pressure. Platelets from patients were permeabilized with saponin and the rate of ATP-driven thapsigargin-sensitive Ca2+ uptake determined using the fluorescent Ca2+ indicator fluo-3. No relationship between blood pressure (systolic, diastolic, day, night) and the rate of Ca2+ uptake into the sacroplasmic reticulum of platelets was found. A weak but insignificant correlation between Ca2+ uptake and the heart rate was noted. Therefore, the increase in cytosolic Ca2+ of platelets in hypertension may not be due to changes of the activity of Ca2+ uptake into the sacroplasmic reticulum.
Eberhard
M
M
Department of Research, Kantonsspital, Basel, Switzerland.
Evéquoz
D
D
Erne
P
P
D000328:Adult; D000814:Aniline Compounds; D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D002118:Calcium / Q000097:blood*; D005260:Female; D005456:Fluorescent Dyes; D006339:Heart Rate; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000503:physiopathology; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium