Molecular cloning and expression of rat liver aminopeptidase B.
We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNAs, a cDNA clone with 2212 base pairs encoding aminopeptidase B (EC 3.4.11.6). The open reading frame encodes a 649-amino acid protein with a theoretical molecular mass of 72,545 Da and bears the consensus sequence of the zinc metalloexopeptidases, indicating that the enzyme belongs to this family, which includes aminopeptidase A, aminopeptidase N, and leukotriene-A4 hydrolase. Escherichia coli SOLR cells infected with the pBluescript phagemid excised from the Uni-ZAP XR vector containing the aminopeptidase B cDNA had a high L-arginyl-beta-naphthylamidase activity. The recombinant protein was purified to homogeneity from the recombinant E. coli extracts. The enzyme had Cl--dependent aminopeptidase activity specifically restricted to the Arg and Lys derivatives and contained 1 mol of zinc per mol of the enzyme.
Fukasawa
K M
KM
Department of Oral Biochemistry, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan.
Fukasawa
K
K
Kanai
M
M
Fujii
S
S
Harada
M
M
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004851:Epoxide Hydrolases / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000201:enzymology; D008667:Metalloproteins / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D015032:Zinc
D018076:DNA, Complementary; D008667:Metalloproteins; D004851:Epoxide Hydrolases; D000626:Aminopeptidases; C019721:aminopeptidase B; D015032:Zinc; C043134:leukotriene A4 hydrolase