title: Coupled-column chromatography on a Chiral-AGP phase for determination of amlodipine enantiomers in human plasma: an HPLC assay with electrochemical detection.
abstract: A sensitive enantioselective high performance liquid chromatographic assay for determination of the dihydropyridine-type calcium antagonist amlodipine in human plasma samples is described. Chiral chromatography is performed on an alpha 1-acid glycoprotein column (i.e. Chiral-AGP) and the eluted enantiomers are trapped and compressed on two short columns before final achiral chromatography on a narrow bore column (i.e. Zorbax SB-Ph) using electrochemical detection. Both stereoselective quantitative analysis and enantiomeric ratio analysis, for samples with a known total concentration of amlodipine are described. The quantitative assay shows linearity over the range 0.5-10 ng ml-1 for the two enantiomers and the limit of detection is about 0.2 ng ml-1. The method has been applied to a pharmacokinetic study of the enantiomers of amlodipine in human subjects.
authors.affiliation: Department of Clinical Pharmacology, Faculty of Health Sciences, Linköping University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Josefsson
authors.forename: B
authors.initials: B
authors.lastname: Norlander
mesh_terms: D017311:Amlodipine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009961:Orosomucoid; D013237:Stereoisomerism
chemical_list: D009961:Orosomucoid; D017311:Amlodipine