Estrogenicity of resin-based composites and sealants used in dentistry.
We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion in human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to bisphenol-A and bisphenol-A dimethacrylate, monomers found in the base paste of the dental sealant and identified by mass spectrometry. Samples of saliva from 18 subjects treated with 50 mg of a bis-GMA-based sealant applied on their molars were collected 1 hr before and after treatment. Bisphenol-A (range 90-931 micrograms) was identified only in saliva collected during a 1-hr period after treatment. The use of bis-GMA-based resins in dentistry, and particularly the use of sealants in children, appears to contribute to human exposure to xenoestrogens.
Olea
N
N
Department of Radiology, Dentistry, and Department of Nutrition, School of Pharmacy, University of Granada, Spain.
Pulgar
R
R
Pérez
P
P
Olea-Serrano
F
F
Rivas
A
A
Novillo-Fertrell
A
A
Pedraza
V
V
Soto
A M
AM
Sonnenschein
C
C
D000328:Adult; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate / Q000378:metabolism / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division; D005260:Female; D006801:Humans; D008297:Male; D010636:Phenols / Q000378:metabolism / Q000494:pharmacology*; D010895:Pit and Fissure Sealants / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000819:agonists* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000096:biosynthesis; D012463:Saliva / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D010636:Phenols; D010895:Pit and Fissure Sealants; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds