Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression.
An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Pol into the attB site of phage lambda in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage lambda.
Fedorova
N D
ND
Engelhardt Institute of Molecular Biology, Moscow, Russia.
Peredelchuk
M Y
MY
Kirpichnikov
M P
MP
Bennett
G N
GN
D017123:Bacteriophage T7 / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D010957:Plasmids; D013045:Species Specificity
D012321:DNA-Directed RNA Polymerases