Endothelin-stimulated phosphoinositide turnover and protein kinase C translocation in rat synaptosomes.
The action of endothelin-1 (ET-1) on phosphoinositide metabolism was studied in rat synaptosomes. ET-1 caused an early and transitory decrease of 32P incorporation into phosphoinositides, concomitantly with an increase into phosphatidic acid (PA). This effect was time-dependent and was not found in the absence of exogenous calcium. Furthermore, ET-1 caused an increase in the generation of inositol phosphates and diacylglycerol (DAG). In addition, the peptide provoked a translocation of protein kinase C from the cytosol to membrane.
Catalan
R E
RE
Departamento de Biologia Molecular, Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain.
Martinez
A M
AM
Aragones
M D
MD
Fernandez
I
I
Miguel
B G
BG
Perez
M J
MJ
Calcerrada
M C
MC
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex; D003593:Cytoplasm / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
D004075:Diglycerides; D019332:Endothelin-1; D007295:Inositol Phosphates; D010710:Phosphates; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D011493:Protein Kinase C; D002118:Calcium