Direct interaction between the envelope and matrix proteins of HIV-1.
The incorporation of the envelope (env) glycoprotein of the human immunodeficiency virus type 1 (HIV-1) into budding virions has been proposed to be mediated by an interaction between its cytoplasmic domain and the matrix protein of HIV-1. However, this interaction was never directly demonstrated and its role in the biogenesis of HIV-1 virions is still debated. Here, a direct interaction is reported between the matrix protein of HIV-1 and the cytoplasmic domain of the env protein of HIV-1. No interaction was seen with the env cytoplasmic domain of other retroviruses. The region of the HIV-1 env involved in the interaction was delineated by mutagenesis and is comprised of the C-terminal 67 amino acid residues of env. These results, as well as the analysis of mutants of the matrix protein, suggest that the interaction between the HIV-1 env and matrix proteins accounts for the specific incorporation of the env glycoprotein into HIV-1 virions.
Cosson
P
P
Basel Institute for Immunology, Switzerland.
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D015686:Gene Products, env / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
D015686:Gene Products, env; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins