Characterization of isogenic carp (Cyprinus carpio L.) lines with a genetically determined high or low antibody production.
Antibody production to dinitrophenyl-keyhole limpet haemocyanin (DNP-KLH) served as the immune parameter to divergently select carp (Cyprinus carpio L.) to produce high- and low-responder F1 hybrid lines. Antibody production to trinitrophenyl-lipopolysaccharide (TNP-LPS) and to DNP-KLH were similar in magnitude. By contrast, some high-responder lines were low responders to DNP-human serum albumin, and vice versa. Low-responder carp were relatively susceptible to infection with the parasite Trypanoplasma borreli. This suggested that at least one gene with a major influence on resistance differed between the two homozygous parents (69, 85) used to generate the high- and low-responder homozygous families, respectively. The isogenic lines showed no within-line variation in DNA fingerprints, but differed with respect to their MhcCyca-DAB genes.
Wiegertjes
G F
GF
Department of Experimental Animal Morphology and Cell Biology, AH Wageningen, The Netherlands.
Bongers
A B
AB
Voorthuis
P
P
Zandieh Doulabi
B
B
Groeneveld
A
A
Van Muiswinkel
W B
WB
Stet
R J
RJ
D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D002347:Carps / Q000235:genetics* / Q000276:immunology; D003433:Crosses, Genetic; D017931:DNA Primers; D004140:Dinitrophenols / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D005393:Fish Diseases; D005802:Genes, MHC Class II; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006720:Homozygote; D006801:Humans; D016830:Kinetoplastida; D008297:Male; D016133:Polymerase Chain Reaction; D011528:Protozoan Infections / Q000276:immunology; D011529:Protozoan Infections, Animal; D012709:Serum Albumin / Q000276:immunology
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D017931:DNA Primers; D004140:Dinitrophenols; D006241:Haptens; D000949:Histocompatibility Antigens Class II; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D006433:Hemocyanins