Partial cDNA sequencing of mouse dentine sialoprotein and detection of its specific expression by odontoblasts.
Dentine sialoprotein (DSP), a 53-kDa acidic glycoprotein, is expressed by odontoblasts and secreted into the dentine extracellular matrix. Although little is known about its biological function, it might play a part in dentinogenesis. Because DSP has only been shown to occur in rat dentine, it is important to demonstrate its existence in another species. Here, the presence of DSP gene in the mouse genome, and the cloning of a mouse DSP cDNA coding for about one-fifth of the molecule with a nucleotide sequence similar to that for rat cDNA, are reported. Using in-situ hybridization, DSP mRNA was uniquely detected in mouse odontoblasts.
Ritchie
H H
HH
Department of Basic Sciences, University of Texas Houston Health Science Center 77030, USA.
Shigeyama
Y
Y
Somerman
M J
MJ
Butler
W T
WT
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D003804:Dentin / Q000166:cytology / Q000378:metabolism*; D003810:Dentinogenesis / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016678:Genome; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009804:Odontoblasts / Q000378:metabolism*; D010750:Phosphoproteins; D011498:Protein Precursors; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D000099311:Dentin Sialophosphoprotein
D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein