Determination of tetracyclines in animal feeds in the presence of other drugs by thin-layer chromatography and microbiological method.
This method was developed to separate, detect, and quantitate oxytetracycline (OTC) or chlortetracycline hydrochloride (CTC.HCl) in animal feeds in the presence of 11 other drugs: 3 nitrofurans, 2 macrolide antibiotics, 3 sulfonamides, 2 coccidiostatics, and 1 antibacterial growth promoter. OTC or CTC.HCl was separated from coexisting drugs and detected by thin-layer chromatography, then quantitated microbiologically by the agar diffusion method. Analysis of 125 experimental animal feed samples fortified at 5 levels (7.5-400 ppm) with OTC or CTC.HCl and at 1 level (50 ppm) with the rest of the drugs, respectively, gave a limit of quantitation of 1.25 or 0.625 ppm, a recovery of 90.6 or 92.9%, and a coefficient of variation of 2.9-6.1 or 2.3-4.4%.
Markakis
P K
PK
Laboratory of Animal Nutrition, Aristotle University, Thessaloniki, Greece.
D000821:Animal Feed / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002751:Chlortetracycline / Q000032:analysis* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods; D004347:Drug Interactions; D008828:Microbiological Techniques; D010118:Oxytetracycline / Q000032:analysis* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D002751:Chlortetracycline; D010118:Oxytetracycline