[10 years' experiences with flow cytometric immunological phenotyping in malignant hematologic diseases].
Immunological phenotyping of mononuclear cell suspensions from bone marrow and/or peripheral blood from 2341 patients (6140 examinations) suspected of malignant haematological diseases was performed during a 10-year period. The cells were labelled with a panel of monoclonal antibodies and subjected to flowcytometry. The results showed a clear distinction between acute lymphoblastic leukaemia and acute myeloid leukaemia, and a new group of acute hybrid leukaemia (3.3% of all acute leukaemia cases) was established. During the 10-year period immunological phenotyping has changed from being a research method to a widely employed method for cell characterisation early in the course of malignant haematological diseases.
Kristensen
J S
JS
Arhus Amtssygehus, medicinsk-haematologisk afdeling.
Hokland
P
P
D000911:Antibodies, Monoclonal; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D010641:Phenotype; D011446:Prospective Studies; D012189:Retrospective Studies
D000911:Antibodies, Monoclonal