title: The expression of complement protein 4 and IgG3 in luminal secretions.
abstract: BACKGROUND
  Factors regulating proximal small-intestinal luminal concentrations of IgG3, the predominant IgG subclass at this site, are unclear. This study determined whether luminal IgG3 concentrations are related to those of complement protein 4 (C4), an acute-phase reactant predominantly derived from local mucosa.
  
  
  METHODS
  Proximal small-intestinal luminal and peripheral blood IgG subclass and C4 concentrations were measured by radial immunodiffusion in 30 adult subjects without predisposition to disturbed mucosal immunity. Mucosal C4 immunoreactivity and the presence or absence of small-intestinal bacterial overgrowth were determined in all subjects. Caecal luminal concentrations of IgG3 and C4 were measured in a separate cohort of eight asymptomatic subjects.
  
  
  RESULTS
  Proximal small-intestinal luminal C4 and IgG subclass concentrations were not significantly influenced by the presence of absence of small-intestinal bacterial overgrowth (P > 0.2). Nor did plasma C4 levels significantly influence C4 concentrations in small-intestinal luminal secretions (P > 0.2). Mucosal immunoreactivity for C4 was present in every subject. A significant correlation was found between C4 and IgG3 concentrations in proximal small-intestinal luminal secretions (P < 0.0005) and also in caecal secretions (P < 0.05) but not in peripheral blood (P > 0.1).
  
  
  CONCLUSIONS
  Common factors, not including the presence or absence of small-intestinal bacterial overgrowth, regulate luminal concentrations of C4 and IgG3. Local investigation is mandatory when assessing mucosal immune mechanisms.
authors.affiliation: Dept. of Gastroenterology, Prince of Wales Hospital, The University of New South Wales, Sydney, Australia.
authors.forename: S M
authors.initials: SM
authors.lastname: Riordan
authors.forename: C J
authors.initials: CJ
authors.lastname: McIver
authors.forename: M C
authors.initials: MC
authors.lastname: Thomas
authors.forename: D
authors.initials: D
authors.lastname: Wakefield
authors.forename: P C
authors.initials: PC
authors.lastname: Andreopoulos
authors.forename: V M
authors.initials: VM
authors.lastname: Duncombe
authors.forename: T D
authors.initials: TD
authors.lastname: Bolin
mesh_terms: D002432:Cecum / Q000276:immunology / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism; D007419:Intestinal Secretions / Q000276:immunology / Q000378:metabolism*; D007421:Intestine, Small / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D008875:Middle Aged
chemical_list: D003181:Complement C4; D007074:Immunoglobulin G