Distribution and processing of peptidylglycine alpha-amidating monooxygenase activity in rat dorsal root ganglia and sciatic nerves.
Since peptidylglycine alpha-amidating monooxygenase (PAM) and carboxypeptidase H (CPH) are transported by a rapid anterograde axonal flow in rat sciatic nerves, different properties of those enzymes were examined in the cell body (dorsal root ganglia) and axon (sciatic nerves) of rat. The relative enzyme activities of soluble to membrane-associated forms in the sciatic nerves were higher than those in the ganglia. On a gel permeation chromatogram, 3 peaks (100, 70 and 30 kDa) of PAM activity appeared in both tissues. There are main peaks at 70 kDa in the ganglia and at 30 kDa in the sciatic nerves. From these data, we suppose that PAM in rat sciatic nerves is proteolytically processed during the axonal transport of secretion granules.
Takasugi
H
H
Laboratory of Molecular Recognition, Graduate School of Integrated Science, Yokohama City University, Japan.
Mori
M
M
Chikuma
T
T
Kato
T
T
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D005727:Ganglia, Spinal / Q000201:enzymology*; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009046:Motor Neurons / Q000201:enzymology; D009097:Multienzyme Complexes; D009475:Neurons, Afferent / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000201:enzymology*; D012995:Solubility
D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D002268:Carboxypeptidases; D043423:Carboxypeptidase H; D000109:Acetylcholine