title: Detection of cyanobacterial toxins (microcystins) in cell extracts by micellar electrokinetic chromatography.
abstract: A micellar electrokinetic chromatography (MEKC) method with UV detection is described for the rapid and efficient separation of three microcystins: microcystin-YR (MCYST-YR), microcystin-LR (MCYST-LR) and microcystin-RR (MCYST-RR). A detection limit of 7.5 pg for each toxin was achieved. The UV intensities of these toxins measured at 200 nm showed good linearity in the range 7.5-150 pg. The production of MCYST-LR in three cultured strains of cyanobacteria, namely Microcystis aeruginosa strain IP7806, Microcystis aeruginosa strain IP7813 and Oscillatoria agardhii strain IP7805, was evaluated.
authors.affiliation: CEMATMA, Laboratoire de Chimie Analytique, ESPCI, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Bouaïcha
authors.forename: C
authors.initials: C
authors.lastname: Rivasseau
authors.forename: M C
authors.initials: MC
authors.lastname: Hennion
authors.forename: P
authors.initials: P
authors.lastname: Sandra
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008387:Marine Toxins; D008823:Micelles; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D014466:Ultraviolet Rays
chemical_list: D001427:Bacterial Toxins; D008387:Marine Toxins; D008823:Micelles; D052998:Microcystins; D010456:Peptides, Cyclic; C065685:microcystin YR; C063855:microcystin RR; C057862:cyanoginosin LR