title: A set of compatible tac promoter expression vectors.
abstract: A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacIq gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, University of Western Ontario, London, Canada.
authors.forename: D M
authors.initials: DM
authors.lastname: Dykxhoorn
authors.forename: R
authors.initials: R
authors.lastname: St Pierre
authors.forename: T
authors.initials: T
authors.lastname: Linn
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018741:Replication Origin