Insight into the distribution of molecular weights and higher-order structure of hyaluronans and some beta-(1 --> 3)-glucans by size exclusion chromatography.
The effects of high energy ultrasound and slightly raised temperature combined with the denaturing action of dimethylsulphoxide on the molecular weight and higher-order structure of hyaluronans and some beta-(1 --> 3)-glucans were studied by means of size exclusion chromatography (SEC) technique. Some experimental conditions connected with the (bio-)polymer sample preparation prior to its SEC analysis are overviewed in the light of informational relevance of studies where the action of a physical and/or chemical agent changes the hydrodynamic size of the m omolecule.
Soltés
L
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Mislovicová
D
D
Sébille
B
B
D002138:Calibration; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D007912:Lentinan / Q000032:analysis; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry; D012566:Sizofiran / Q000032:analysis; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics; D047071:beta-Glucans
D005936:Glucans; D006025:Glycosaminoglycans; D011134:Polysaccharides; D047071:beta-Glucans; D007912:Lentinan; D012566:Sizofiran; D006820:Hyaluronic Acid; C033363:beta-1,3-glucan