Quantification of mRNAs for three alpha 1-adrenoceptor subtypes in rat aorta by solution hybridization.
We used solution hybridization to quantify mRNAs for alpha 1A-, alpha 1B-and alpha 1D-adrenoceptor(AR) in rat aortae. The results showed that the mRNA for alpha 1A-, alpha 1B- and alpha 1D-AR was 0.28 +/- 0.03 fmol, 0.35 +/- 0.03 fmol and 0.82 +/- 0.09 fmol, respectively, in 40 micrograms total RNA extracted from rat aorta. The dominant expression of alpha 1D-AR further support the recent findings that alpha 1D-AR is a major functional receptor subtype of AR in rat aorta.
Xu
K
K
Institute of Vascular Medicine, Third Hospital, Beijng Medical University, China.
Han
C
C
D000818:Animals; D001011:Aorta / Q000737:chemistry*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis* / Q000235:genetics; D012260:Ribonucleases / Q000378:metabolism
C547715:Adra1a protein, rat; C547718:Adra1b protein, rat; C547721:Adra1d protein, rat; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D012260:Ribonucleases