In vitro blood compatibility evaluation of hollow fibre membrane using a controlled flow system: a comparative study.
A procedure has been established for the in vitro assessment of hollow fibre haemodialysis membranes. A 30 ml syringe containing 20 ml of fresh non-anticoagulated blood was mounted onto a non-pulsatile syringe pump and blood was perfused through minimodules constructed from 80 fibres retrieved from Cuprophan (Baxter ST15), cellulose acetate (M57-12, JMS Co Ltd, Hiroshima, Japan), and AN69HF (Filtral 20, Hospal, France) dialysers. Samples were collected before perfusion, 3, 6, 9 and 12 minutes. The modules were clamped vertically to minimise the effect of red cell pooling and the dialysate compartment was filled with 0.9% saline to minimise ultrafiltration. After sample processing, complement C3a, thrombin-antithrombin III complexes, prothrombin F1 + 2, and factor XII-like activity were evaluated. The results indicated that the system could discriminate between the membranes evaluated and therefore was a relevant procedure for the assessment of hollow fibre haemodialysis membranes.
Matata
B M
BM
Bioengineering Unit, University of Strathclyde, Glasgow, UK.
Yin
H Q
HQ
Courtney
J M
JM
Gaylor
J D
JD
Lamba
N M
NM
Lowe
G D
GD
Suzuki
K
K
Kimura
H
H
Izumi
K
K
Klinkmann
H
H
D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials / Q000592:standards*; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D001800:Blood Specimen Collection; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism; D005174:Factor XII / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D010447:Peptide Hydrolases / Q000378:metabolism; D011516:Prothrombin / Q000032:analysis; D011673:Pulsatile Flow; D006435:Renal Dialysis / Q000592:standards*; D013499:Surface Properties
D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; C046193:antithrombin III-protease complex; C005062:acetylcellulose; D015926:Complement C3a; D000990:Antithrombin III; D011516:Prothrombin; D005174:Factor XII; D002482:Cellulose; C009865:cuprammonium cellulose; D010447:Peptide Hydrolases