Growth hormone and insulin-like growth factor I augment Escherichia coli-killing activity of murine peritoneal exudative cells.
Effects of growth hormone (GH) and insulin-like growth factor (IGF)-I on Escherichia coli-killing activity of murine peritoneal exudative cells (PECs) were investigated. Plasma from the mice, injected subcutaneously with saline, GH (4.8 mg/kg/day), or IGF-I(24 mg/kg/day) for 6 days, was mixed with E. coli and pooled murine PECs. Plasma from GH- and IGF-I-treated mice modestly but significantly augmented the E. coli-killing activity of PECs, as compared with that from saline controls. Plasma from IGF-I-treated mice also enhanced PEC interleukin 1 production. In the next experiment, PECs preincubated with medium, GH (10-1000 ng/mL), or IGF-I (50-5000 ng/mL) for 3 h were investigated for E. coli-killing activity. Preincubation of PECs with all concentrations of GH and IGF-I significantly enhanced the E. coli-killing activity of PECs, as compared with the medium control. These results indicate that GH and IGF-I enhance phagocytosis and the E. coli-killing activity of PECs, via a modestly increased plasma capacity to support these activities, as well as by a strong direct action.
Inoue
T
T
Department of Surgery I, University of Tokyo, Japan.
Saito
H
H
Hashiguchi
Y
Y
Fukatsu
K
K
Inaba
T
T
Lin
M T
MT
Han
I
I
Furukawa
S
S
Muto
T
T
D000818:Animals; D001770:Blood Bactericidal Activity / Q000187:drug effects; D002364:Caseins / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects*; D005122:Exudates and Transudates / Q000187:drug effects / Q000502:physiology*; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology* / Q000382:microbiology; D011492:Protein Hydrolysates / Q000494:pharmacology
D002364:Caseins; D016207:Cytokines; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone