title: Body cavity-based malignant lymphoma containing Kaposi sarcoma-associated herpesvirus in an HIV-negative man with previous Kaposi sarcoma.
abstract: BACKGROUND
  The role of Kaposi sarcoma-associated herpesvirus in the development of malignant lymphomas in patients negative for the human immunodeficiency virus (HIV) has not been established.
  
  
  OBJECTIVE
  To examine the possible role of Kaposi sarcoma-associated herpesvirus in a case of body cavity-based malignant lymphoma that occurred in an HIV-negative patient who had previously had Kaposi sarcoma.
  
  
  METHODS
  Case study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  A 94-year-old man with lymphomatous ascites.
  
  
  METHODS
  Polymerase chain reaction (PCR) and Southern blot DNA analysis.
  
  
  RESULTS
  The body cavity-based lymphoma cells were positive for Kaposi sarcoma-associated herpesvirus by PCR and were negative for other herpesviruses, including Epstein-Barr virus, cytomegalovirus, and human herpesviruses 6 and 7. Southern blot analysis of lymphoma DNA showed high levels of Kaposi sarcoma-associated herpesvirus (> 40 to 80 genomes/cell). Clonal rearrangement of the immunoglobulin JH and JK genes was present, confirming the presence of a clonal B-cell proliferation.
  
  
  CONCLUSIONS
  Kaposi sarcoma-associated herpesvirus may be involved in the development of malignant lymphoma after Kaposi sarcoma in HIV-negative patients. This type of lymphoma, in contrast to body cavity-based lymphoma related to the acquired immunodeficiency syndrome, may have an indolent clinical course.
authors.affiliation: Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Strauchen
authors.forename: A D
authors.initials: AD
authors.lastname: Hauser
authors.forename: D
authors.initials: D
authors.lastname: Burstein
authors.forename: R
authors.initials: R
authors.lastname: Jimenez
authors.forename: P S
authors.initials: PS
authors.lastname: Moore
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D015322:Gene Rearrangement, B-Lymphocyte; D018023:HIV Seronegativity; D006564:Herpesviridae / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000235:genetics / Q000821:virology*; D008297:Male; D016609:Neoplasms, Second Primary / Q000235:genetics / Q000821:virology*; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*
chemical_list: D004279:DNA, Viral