Characterization of cystatin C from bovine parotid glands: cysteine proteinase inhibition and antiviral properties.
Cystatin C, a low Mr cysteine proteinase inhibitor was isolated from bovine parotid glands by a procedure which includes alkaline treatment of the homogenate, affinity chromatography, gel filtration and ion exchange chromatography. The purified inhibitor has a pl of 8.0 and Mr of 14500. The identity with bovine cystatin C from colostrum was confirmed by N-terminal sequence of the inhibitor and amino acid composition. Cystatin C rapidly (kass = 5.5 x 10(7) M-1s-1) and tightly inhibits papain (Ki = 0.02 nM), whereas its interaction with bovine cathepsin B is substantially weaker (Ki = 4.4 nM). Bovine cystatin C also shows a weak antiviral effect on poliovirus infected human Hela cells.
Cimerman
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N
Research and Development Division, KRKA, Novo mesto, Slovenia.
Kosorok
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MD
Korant
B D
BD
Turk
B
B
Turk
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D055316:Cystatin C; D015891:Cystatins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010306:Parotid Gland / Q000737:chemistry*; D017955:Poliovirus / Q000187:drug effects; D010948:Viral Plaque Assay
D000998:Antiviral Agents; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors