title: Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa (Pa) strain PAO synthesises a siderophore, pyoverdine (Pvd), when grown under conditions of iron starvation. Pvd consists of a dihydroxyquinoline group attached to an 8-amino-acid-residue peptide. DNA spanning at least 78 kb of the chromosome is required for Pvd synthesis, but to date only three genes involved in this process have been characterised. We report the characterisation of a fourth Pa gene, pvdE, which we show to be required for Pvd synthesis. The deduced amino acid sequence of PvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: B J
authors.initials: BJ
authors.lastname: McMorran
authors.forename: M E
authors.initials: ME
authors.lastname: Merriman
authors.forename: I T
authors.initials: IT
authors.lastname: Rombel
authors.forename: I L
authors.initials: IL
authors.lastname: Lamont
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009842:Oligopeptides; D010860:Pigments, Biological; C102466:PvdE protein, Pseudomonas aeruginosa; D017262:Siderophores; C042453:pyoverdin