Purification and properties of extracellular glucosyltransferase from Streptococcus bovis.
Eight Streptococcus bovis strains were classified into 3 types on the basis of isoelectric point (pI) and molecular mass (M(r)) of extracellular glucosyltransferase. Strains ATCC 9809, 35034 and 43143 produced glucosyltransferase of pI 3.7 and M(r) 165 kDa; strains ATCC 15351, 27960 and 33317 produced glucosyltransferase of pI 4.1 and M(r) 140 kDa; strains ATCC 43085 and 43144 did not produce any glucosyltransferase. The glucosyltransferase form S. bovis 9809 was purified by Bio-Gel hydroxyapatite chromatography and DEAE-Toyopearl chromatography. The S. bovis 9809 glucosyltransferase was immunologically identical with the other 5 S. bovis glucosyltransferases and not related to mutants streptococcal glucosyltransferases. The specific activity, the optimum pH and the Km value for sucrose were 17.9 U/mg protein, 6.0 and 5.0 mM, respectively. The first 11 N-terminal amino acid residues of the glucosyltransferases were DETSAVTLTRE, and the region was hydrophilic. The glucosyltransferases from S. bovis 9809 and 3317 synthesized from sucrose 1, 6-alpha-D-glucan with 9 and 2 mol%, 1, 3, 6-alpha-branched glucose, respectively.
Uezono
Y
Y
Department of Chemistry, National Defense Medical College, Saitama, Japan.
Tsumori
H
H
Shimamura
A
A
Mukasa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002417:Cattle; D003911:Dextrans / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008353:Mannitol / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013291:Streptococcus / Q000145:classification / Q000201:enzymology*
D001426:Bacterial Proteins; D003911:Dextrans; D005936:Glucans; D012967:Sodium Dodecyl Sulfate; D008353:Mannitol; D005964:Glucosyltransferases