Limited T helper cell activity in C57BL/10 (B10) mice with inherited low IgG responsiveness.
Due to limitations in antigen processing, mice of the C57BL/10ScSn (B10) strain exhibit a low IgG production against a variety of T-dependent antigens. To characterize the T-cell functions, the authors studied antigen-specific T-cell proliferation and cytokine production in vitro. The response of B10 mice was compared with that of the high IgG producing strain A/J. A highly restricted proliferative response and almost no interleukin-2 (IL-2) and interleukin-3 (IL-3) production was detected in lymph node (LN) cells of B10 mice primed in vivo by protein antigens and subjected to a specific restimulation in vitro, whilst A/J cells responded by significant proliferation and cytokine production. The antigen-specific T-cell response of B10 mice could not be increased by lipopolysaccharide treatment in vivo or by in vitro cultivation with IL-2. However, the T cells of B10 mice produced high levels of IL-2 and IL-4 when stimulated by phorbol myristate acetate (PMA) and Ca2+ ionophore, proving the existence of a functionally intact signal transduction pathway downstream of protein kinase C (PKC). The results suggest that the in vivo antigen priming does not effectively activate the T cells in B10 mice. The limited activation consequently leads to the low IgG response described in B10 mice.
Sírová
M
M
Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Ríha
I
I
Ríhová
B
B
D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D017951:Antigen Presentation / Q000235:genetics*; D000941:Antigens / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002645:Chickens; D004527:Egg Proteins / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D007376:Interleukin-2 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009113:Muramidase / Q000276:immunology; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000941:Antigens; D004527:Egg Proteins; D007074:Immunoglobulin G; D007376:Interleukin-2; D007377:Interleukin-3; D007476:Ionophores; D008070:Lipopolysaccharides; D015847:Interleukin-4; D000001:Calcimycin; D011493:Protein Kinase C; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium