title: Expression of LAR-PTP2 in rat lung is confined to proliferating epithelia lining the airways and air sacs.
abstract: The LAR family tyrosine phosphatase LAR-PTP2B (RPTP sigma) was previously shown to be expressed in the central and peripheral nervous system. Here we show that LAR-PTP2, the larger alternatively spliced form of the gene, is expressed in proliferating undifferentiated lung epithelia in a developmentally regulated manner: Using in situ hybridization and parallel immunostaining with proliferating cell nuclear antigen to detect proliferating cells, we demonstrate that LAR-PTP2 is expressed exclusively in the undifferentiated epithelial cell layer lining the bronchi, bronchioles, and air sacs in late fetal development and in the neonatal lung. These cells correspond to Clara and fetal alveolar type II cells, as determined by parallel immunostaining with antibodies to surfactant proteins A and B. LAR-PTP2 expression declined progressively with postnatal development, and by adult stage there was no detectable expression in the airways or in the distal (type I and II) mature nonproliferating alveolar epithelial cells. These results suggest that LAR-PTP2 may be involved in the regulation of epithelial cell proliferation/differentiation during lung development.
authors.affiliation: Medical Research Council Group in Lung Development, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Yeger
authors.forename: R
authors.initials: R
authors.lastname: Han
authors.forename: M
authors.initials: M
authors.lastname: Wallace
authors.forename: B
authors.initials: B
authors.lastname: Goldstein
authors.forename: D
authors.initials: D
authors.lastname: Rotin
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D004848:Epithelium / Q000378:metabolism; D017403:In Situ Hybridization; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D017027:Protein Tyrosine Phosphatases; D051381:Rats; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D017027:Protein Tyrosine Phosphatases; C516774:Ptprf protein, rat; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2