title: Rapid construction of three-fragment recombinant DNAs by polymerase chain reaction: application for gene-targeting in Saccharomyces cerevisiae.
authors.affiliation: Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Ling
authors.forename: B H
authors.initials: BH
authors.lastname: Robinson
mesh_terms: D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction / Q000379:methods; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D011768:Pyruvate Dehydrogenase Complex