Whole saliva dried on filter paper or diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen.
Whole saliva samples collected from HIV-1 seropositive subjects by simple spitting without using any devices were dried on filter paper strips, from which filter paper discs of 3-mm diameter were punched out. The eluates of the discs were subjected to the immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 using recombinant reverse transcriptase of HIV-1 as antigen and a two-site enzyme immunoassay for whole IgG. The signals for antibody IgG to HIV-1 and the amounts of whole IgG obtained with one disc per assay tube were 126-290% of those obtained with 1 microliter of whole saliva samples, provided that filter paper strips were treated with nonspecific rabbit serum prior to drying whole saliva samples and that filter paper discs were tested within a few days after drying whole saliva samples. From these results, diagnosis of HIV-1 infection was indicated to be possible with whole saliva samples dried on filter papers, since the diagnosis was previously shown to be possible with 1 microliter of whole saliva samples. The test for HIV-1 infection with whole saliva samples dried on filter papers was suggested to be useful for various purposes.
Ishikawa
S
S
Department of Biochemistry, Miyazaki Medical College, Miyazaki, Japan.
Hashida
S
S
Hashinaka
K
K
Hirota
K
K
Kojima
M
M
Saito
A
A
Takamizawa
A
A
Shinagawa
H
H
Oka
S
S
Shimada
K
K
Ishikawa
E
E
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D005374:Filtration / Q000295:instrumentation; D054303:HIV Reverse Transcriptase / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology; D012463:Saliva / Q000276:immunology / Q000821:virology*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology; D001616:beta-Galactosidase
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014764:Viral Proteins; D054303:HIV Reverse Transcriptase; D001616:beta-Galactosidase