Chaperone function of Hsp90-associated proteins.
The Hsp90 heat shock protein of eukaryotic cells regulates the activity of proteins involved in signal transduction pathways and may direct intracellular protein folding in general. Hsp90 performs at least part of its function in a complex with a specific set of partner proteins that include members of the prolyl isomerase family. The properties of the major components of the Hsp90 complex were examined through the use of in vitro protein folding assays. Two of the components, FKBP52 and p23, functioned as mechanistically distinct molecular chaperones. These results suggest the existence of a super-chaperone complex in the cytosol of eukaryotic cells.
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Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, 93040 Regensburg, Germany. johannes.buchner@biologie.uni-regensburg.de
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D000591:Amino Acid Isomerases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D002950:Citrate (si)-Synthase / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D019746:Intramolecular Oxidoreductases; D053612:Janus Kinases; D018832:Molecular Chaperones / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D019696:Peptidylprolyl Isomerase; D010750:Phosphoproteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D011090:Polyenes / Q000494:pharmacology; D000071876:Prostaglandin-E Synthases; D011489:Protein Denaturation; D017510:Protein Folding; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D020123:Sirolimus; D022021:Tacrolimus Binding Proteins; D013696:Temperature; D014157:Transcription Factors
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010750:Phosphoproteins; D011090:Polyenes; D011994:Recombinant Proteins; D014157:Transcription Factors; D002950:Citrate (si)-Synthase; D011505:Protein-Tyrosine Kinases; D053612:Janus Kinases; C085845:hop protein, Drosophila; D000591:Amino Acid Isomerases; D022021:Tacrolimus Binding Proteins; D019696:Peptidylprolyl Isomerase; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D020123:Sirolimus