High-performance liquid chromatographic determination of vancomycin in rabbit serum, vitreous and aqueous humour after intravitreal injection of the drug.
A high-performance liquid chromatographic method for the determination of vancomycin in rabbit serum, vitreous and aqueous humour has been developed. No clean-up step was necessary for vitreous and aqueous humour samples. For serum samples liquid-liquid and solid-phase extraction were tested and the best results were achieved using C18 cartridges. The extracts were analyzed on a C18 reversed-phase column, using a mixture of 0.05 M phosphate buffer (pH 4) with 10% of acetonitrile as mobile phase. The detection was carried out at 198 nm, which allows higher sensitivity. The average quantitation limit obtained was 0.03 micrograms/ml. The method has been applied to the study of the residual quantities of vancomycin in serum and rabbit eyes after intravitreal administration of the drug in endophthalmitis treatment.
Del Nozal
M J
MJ
Department of Analytical Chemistry, University de Valladolid, Spain.
Bernal
J L
JL
Pampliega
A
A
Marinero
P
P
López
M I
MI
Coco
R
R
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D013696:Temperature; D014640:Vancomycin / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D014640:Vancomycin