"In vivo" and "in vitro" antitumoral action of Larrea divaricata Cav.
We previously reported that aqueous extract of Larrea divaricata Cav. had an antiproliferative activity upon tumoral lymphoid cells (BW 5147), without affecting normal immunity. To determine the probable mechanism of the inhibitory action of the extract upon cell growth, the participation of intracellular signals involved in the inhibition of cell proliferation, namely the activation of adenylate cyclase system was studied. The production of cyclic 3', 5 adenosine monophosphate (cAMP) in presence and absence of extract was analized. The extract increased the cAMP levels, but neither the cAMP production nor the inhibitory effect of the extract on proliferation were blocked by a beta adrenergic receptor antagonist (propranolol) or by histaminergic receptor antagonists (cimetidine and mepyramine). So, we concluded that the antiproliferative activity of the extract of BW 5147 cells would be mediated by an increase in cAMP intracellular levels no related to the activation of the membrane receptors here studied. In parallel, the extract was administered to a pregnant rat with a spontaneous mammarian carcinoma and "in vivo" antitumoral activity was found.
Anesini
C
C
Departament of Pharmacology, Buenos Aires University, Argentina.
Genaro
A
A
Cremaschi
G
G
Zubillaga
M
M
Boccio
J
J
Sterin-Borda
L
L
Borda
E
E
D000704:Analysis of Variance; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D002277:Carcinoma / Q000188:drug therapy; D002455:Cell Division / Q000187:drug effects*; D002927:Cimetidine / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000494:pharmacology; D016399:Lymphoma, T-Cell / Q000188:drug therapy; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D011247:Pregnancy; D011433:Propranolol / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D013936:Thymidine / Q000037:antagonists & inhibitors
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D002927:Cimetidine; D006632:Histamine; D011433:Propranolol; D000242:Cyclic AMP; D011738:Pyrilamine; D013936:Thymidine