Genetic and biochemical probes for protein-protein interactions.
The two-hybrid system is a powerful approach for examining protein-protein interactions. Recently, the utility of the system has been extended to include the genome-wide mapping of protein-protein interactions and the identification of peptide inhibitors of protein interactions. In addition, immunophilins and their chemical ligands are providing useful reagents for generating conditional protein-protein interactions in vivo to dissect intracellular signaling pathways.
McNabb
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DS
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. dsmcnabb@mit.edu
Guarente
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L
D000818:Animals; D001709:Biotechnology; D015335:Molecular Probes; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction
D015335:Molecular Probes; D011506:Proteins; D011994:Recombinant Proteins