Evidence for cholecystokininA receptors in bovine adrenal chromaffin cells.
We have studied a possible role of cholecystokinin (CCK) in regulating adrenal medullary function. Caerulein (10(-10)-10(-7) M), a CCK receptor agonist, increased formation of inositol phosphates in primary cultured bovine adrenal medullary (BAM) chromaffin cells in a concentration-dependent manner. The effect of caerulein was antagonized by devazepide, a selective CCKA-receptor antagonist, but not by L-365.260, a selective CCKB-receptor antagonist. These results suggest that BAM cells possess functional CCK receptors of the CCKA-subtype. Stimulation of these receptors with caerulein activates a signal transduction pathway via phospholipase C. CCK may regulate catecholamine release in BAM cells.
Aarnisalo
A M
AM
Department of Pharmacology and Toxicology, University of Helsinki, Finland.
Vainio
P J
PJ
Männistö
P T
PT
Vasar
E
E
Tuominen
R K
RK
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002108:Ceruletide / Q000494:pharmacology*; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D020109:Devazepide; D006727:Hormone Antagonists / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D010671:Phenylurea Compounds; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*; D013758:Tetragastrin / Q000494:pharmacology
D001570:Benzodiazepinones; D006727:Hormone Antagonists; D007295:Inositol Phosphates; D010671:Phenylurea Compounds; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin; D013758:Tetragastrin; C058121:L 365260; D002108:Ceruletide; D020109:Devazepide