title: Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene.
abstract: A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.
authors.affiliation: Department of Molecular Immunology, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: I
authors.initials: I
authors.lastname: Inoue
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004285:Dogs; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011970:Receptors, Histamine H2 / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D012333:RNA, Messenger; D011970:Receptors, Histamine H2