title: Effective vaccination of mice against Mycobacterium leprae with density-gradient subfractions of soluble M. leprae proteins: clues to effective protein epitopes.
abstract: It had previously been discovered that intradermal mouse vaccination with a protein fraction of Mycobacterium leprae (called soluble proteins) in Freund's incomplete adjuvant (FIA) resulted in consistent and long-lived protection against M. leprae multiplication from subsequent viable footpad challenges. In this study certain density-gradient subfractions of this soluble protein, but not others, in FIA afforded vaccine protection. The results of this study suggest which M. leprae proteins may be involved in protective immunity, particularly 1-3 kD, 10 kD, 65 kD, and those of higher molecular weight.
authors.affiliation: Medical Research Institute of California Pacific Medical Center, San Franscisco, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Gelber
authors.forename: S W
authors.initials: SW
authors.lastname: Hunter
authors.forename: L P
authors.initials: LP
authors.lastname: Murray
authors.forename: P
authors.initials: P
authors.lastname: Siu
authors.forename: M
authors.initials: M
authors.lastname: Tsang
authors.forename: P J
authors.initials: PJ
authors.lastname: Brennan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D004195:Disease Models, Animal; D007271:Injections, Intradermal; D007918:Leprosy / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D009166:Mycobacterium leprae / Q000276:immunology*; D014611:Vaccination
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines