title: Magnetizable solid-phase supports for purification of nucleic acids.
abstract: A simple, small scale non-hazardous procedure for the production of magnetizable solid-phase support (MSPS) beads has been developed based on the extrusion of agarose/iron oxide mixtures. The MSPS beads were derivatized using various amine ligands. Derivatized MSPS beads were used to adsorb nucleic acids from aqueous solution and to separate RNA/DNA mixtures.
authors.affiliation: School of Biological and Chemical Sciences, University of Greenwich, London, UK.
authors.forename: I J
authors.initials: IJ
authors.lastname: Bruce
authors.forename: M J
authors.initials: MJ
authors.lastname: Davies
authors.forename: K
authors.initials: K
authors.lastname: Howard
authors.forename: D E
authors.initials: DE
authors.lastname: Smethurst
authors.forename: M
authors.initials: M
authors.lastname: Todd
mesh_terms: D002847:Chromatography, Agarose / Q000379:methods*; D008280:Magnetics; D009696:Nucleic Acids / Q000302:isolation & purification*; D012685:Sepharose / Q000648:ultrastructure
chemical_list: D009696:Nucleic Acids; D012685:Sepharose