title: Evaluation of an antigen-capture enzyme immunoassay for detection of Entamoeba histolytica in stool samples.
abstract: In order to identify the prevalence of Entamoeba histolytica in tourists with diarrhoea returning from countries of the developing world, sensitivity and specificity of a commercially available enzyme immunoassay (EIA) kit for the detection of Entamoeba histolytica coproantigen in stool were evaluated. Five hundred seventy-seven specimens from 469 patients were examined by microscopy and EIA. Sixty-two specimens from 49 patients were considered positive for Entamoeba histolytica. Compared with microscopic examination of stool samples, the EIA was found to be slightly more sensitive (90.3% vs. 87.1%) and was 97.7% specific for Entamoeba histolytica.
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Jelinek
authors.forename: G
authors.initials: G
authors.lastname: Peyerl
authors.forename: T
authors.initials: T
authors.lastname: Löscher
authors.forename: H D
authors.initials: HD
authors.lastname: Nothdurft
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004748:Entamoeba histolytica / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000276:immunology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006801:Humans; D007124:Immunoenzyme Techniques; D008853:Microscopy; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan