Molecular cloning of the mouse activin beta E subunit gene.
cDNA clones encoding a novel activin beta subunit have been isolated from mouse liver cDNA library. An amino acid homology comparison among members of the TGF-beta superfamily indicates that the open reading frame codes for a new activin/inhibin beta subunit. The predicted mature region of the subunit shows > 60% identity with activin beta C and beta D and > or = 45% identity with activin beta A and beta B, but only 20-40% amino acid sequence identity with other TGF-beta superfamily members. Therefore, the novel subunit has been designated activin beta E. Alignment of the mature growth factor region of the five activin beta subunits revealed that activin beta A and beta B share 63% amino acid identity, while activin beta C, beta D, and beta E share 62% identity. Therefore, activin beta A and beta B are most related to one another, while activin beta C, beta D, and beta E may represent a subset of related sequences.
Fang
J
J
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0417, USA.
Yin
W
W
Smiley
E
E
Wang
S Q
SQ
Bonadio
J
J
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016212:Transforming Growth Factor beta / Q000235:genetics*
D018076:DNA, Complementary; D009842:Oligopeptides; D010455:Peptides; D016212:Transforming Growth Factor beta; C044147:activin B; D028341:Activins; C048617:myelopeptides