Polymerase chain reaction-based polymorphisms in the porcine cholecystokinin (CCK) gene and assignment to chromosome 13.
Polymorphisms were identified in the porcine cholecystokinin (CCK) gene by digestion of products from polymerase chain reaction (PCR) with the restriction enzyme DpnII. Individuals from the European pig gene mapping project (PiGMaP) consortium reference families (eight full-sib families, 91 total progeny) were genotype to determine linkage relationships between the CCK gene and previously mapped loci. Linkage analysis revealed that the CCK gene is located on porcine chromosome 13.
Clutter
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Department of Animal Science, Oklahoma State University, Stillwater 74078-0435, USA.
Sasaki
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Pomp
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D000818:Animals; D002766:Cholecystokinin / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008126:Lod Score; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D002766:Cholecystokinin; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases