Expression of the Arxula adeninivorans glucoamylase gene in Kluyveromyces lactis.
The glucoamylase gene of the yeast Arxula adeninivorans was expressed in Kluyveromyces lactis by using the GAP promoter from Saccharomyces cerevisiae and a multicopy plasmid vector. The transformants secreted 90.1% of the synthesized glucoamylase into the culture medium. The secreted glucoamylase activities are about 20 times higher in comparison to those of Saccharomyces cerevisiae transformants using the same promoter. Secreted glucoamylase possesses identical N-terminal amino acid sequences to those secreted by A. adeninivorans showing that cleavage of the N-terminal signal peptide takes place at the same site. Biochemical characteristics of glucoamylase expressed by K. lactis and A. adeninivorans are very similar.
Bui
D M
DM
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Kunze
I
I
Horstmann
C
C
Schmidt
T
T
Breunig
K D
KD
Kunze
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D005822:Genetic Vectors; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D007716:Kluyveromyces / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic; D015003:Yeasts / Q000201:enzymology* / Q000235:genetics*
D004271:DNA, Fungal; D021382:Protein Sorting Signals; D005087:Glucan 1,4-alpha-Glucosidase