title: Encapsulation of OKT3 cells in hollow fibers.
abstract: Encapsulation of an OKT3 cell line in hollow fibers was evaluated in vitro and in vivo. The cell line is a mouse hybridoma producing immunoglobulin G2a (IgG2a) against CD3 human T lymphocytes and thus may function as a nonspecific activation system of a subpopulation of human T lymphocytes. For encapsulation purpose, hollow fibers of polypropylene K600 PP Accurel (Akzo, Germany) were selected. Hollow fibers were siliconized to improve membrane biocompatibility for in vivo experiments. The siliconized hollow fibers exhibited acceptable diffusive permeability (P) [ml/min/m2] for small solutes (for creatinine, p = 63.9 +/- 2.0, n = 3) and larger solutes (for albumin, p = 16.9 +/- 1.9, n = 3; for IgG, p = 1.0 +/- 0.2, n = 3). The 12 cm long hollow fibers were filled with a suspension of OKT3 cells of an average density of 10(6) cells/ ml, and both ends were sealed. The encapsulated cells were cultivated in RPMI 1640/10% CS medium at 37 degrees C, 5% CO2 for a period of 3 to 4 days. After the culture period, the medium was tested on human peripheral blood lymphocytes for the presence of anti-CD3 antibody and read in a flow FACS-trac cytometer (Becton Dickinson Immunocytochemistry Systems, San Diego, CA). The tightness of hollow fiber sealing was tested with a bubble point method. The number of cells increased after cultivation by four- to nine-fold on average (n = 11). Ten experiments were performed in vivo with OKT3 cells encapsulated in hollow fibers and implanted subcutaneously into mice for 3 days. In 50% of the experiments, some anti-CD3 antigens on human lymphocytes were found; however, the difference, in comparison with control, in percent of CD3+ was insignificant. In conclusion, the hollow fiber method for cultivation of hybridoma cells in vitro allows for separation of cells from the medium containing secreted anti-CD3 antibodies and is effective in maintaining cell viability. In vivo application needs additional study.
authors.affiliation: Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: L H
authors.initials: LH
authors.lastname: Granicka
authors.forename: J W
authors.initials: JW
authors.lastname: Kawiak
authors.forename: E
authors.initials: E
authors.lastname: Glowacka
authors.forename: A
authors.initials: A
authors.lastname: Werynski
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D002417:Cattle; D004058:Diffusion; D006801:Humans; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology*; D007167:Immunotherapy / Q000379:methods; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D016853:Muromonab-CD3 / Q000096:biosynthesis*
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D016853:Muromonab-CD3