Dimethyl sulfoxide decreases interleukin-8-mediated neutrophil recruitment in the airways.
In this study, we investigated the role of dimethyl sulfoxide (DMSO) in inhibiting interleukin-8 (IL-8)-mediated neutrophil recruitment induced by Pseudomonas aeruginosa (PA) bacterial supernatant. First, we tested whether DMSO could inhibit IL-8 production induced by PA in human bronchial epithelial (16-HBE) cells in vitro. In these cells, exposure to PA or H2O2 induced IL-8 production dose dependently, an effect that was inhibited by 1% DMSO at both the protein and RNA level. Second, we tested whether DMSO could block the recruitment of neutrophils induced by PA in a bypassed segment of dog trachea in vivo. PA supernatant was placed in the tracheal segment for 6 h in four dogs, and neutrophil recruitment and IL-8 concentrations were measured in the superfusate. DMSO prevented the recruitment of neutrophils and IL-8 production induced by PA time dependently. The results suggest that DMSO may play an anti-inflammatory role in the airway by inhibiting IL-8 production in epithelial cells.
Massion
P P
PP
Department of Medicine, University of California San Francisco 94143-0130, USA.
Lindén
A
A
Inoue
H
H
Mathy
M
M
Grattan
K M
KM
Nadel
J A
JA
D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D014132:Trachea / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects
D016209:Interleukin-8; D006861:Hydrogen Peroxide; D004121:Dimethyl Sulfoxide