The pleiotropic effect of TPA on in vitro invasion/migration of glioma and melanoma cell lines.
The invasion/migration of two cell lines, melanoma (MEW) and glioma (BT5C), and their counterparts treated for prolonged time with TPA were studied. On Western blots both cell lines expressed alpha, beta I protein kinase C isoforms, whereas beta II and gamma were not detected. The down-regulation of alpha and beta I PKC was observed in glioma cells after long treatment with TPA, whereas the same treatment of melanoma cells did not lead to PKC downregulation. The down-regulation of PKC was accompanied by stimulation of cell migration/invasion through Transwell chambers coated with collagen IV or fibronectin. In the case of melanoma cells treated with TPA, whose PKC was not down-regulated, the inhibition of migration/invasion was observed. The invasive properties of studied cells did not correlate with any conventional PKC isoform expression.
Szaniawska
B
B
Department of Cell Biology, Cancer Center, Warsaw, Poland.
Maternicka
K
K
Kowalczyk
D
D
Miłoszewska
J
J
Janik
P
P
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D015536:Down-Regulation; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007527:Isoenzymes / Q000187:drug effects* / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D002273:Carcinogens; D007527:Isoenzymes; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate