Neuropeptide Y expression in the trigeminal ganglion and mandibular division of the trigeminal nerve after inferior alveolar nerve axotomy in young rats.
Neuropeptide Y (NPY) is a 36-amino-acid peptide residing in sympathetic nerve terminals, originating from the superior cervical ganglion in oral tissues. NPY exerts vasoconstrictor action together with noradrenalin and has been found to inhibit the release of neurotransmitters from primary afferent fibers. During regeneration of the axotomized inferior alveolar nerve (IAN), NPY-immunoreactive (IR) nerve fibers have been shown in the odontoblast layer and dentin, an area normally innervated by afferent nerve fibers. The dynamic shift in neuropeptide expression in the trigeminal ganglion and in the dental pulp was studied by immunohistochemistry 1, 2, 3, and 8 weeks after IAN axotomy. In the ipsilateral first mandibular molar a temporal loss of pulpal sensory nerves lasting for approximately 1 week was found after axotomy. An upregulation of NPY was shown in neurons located in the mandibular area of the trigeminal ganglion, concomitant to a reduction in number of neurons expressing substance P (SP). To study an alternate and possible trigeminal origin of some of the peripheral nerve fibers IR to NPY in the dental pulp, double immunofluorescence labeling was performed for NPY and calcitonin gene-related peptide (CGRP). Coexistence of NPY and CGRP was shown in neurons located in the trigeminal ganglion and in nerve fibers in the tooth pulp during IAN regeneration. Furthermore, retrograde tracing with Fluorogold revealed NPY-IR neurons projecting to the first molar pulp 3 weeks after axotomy. Hence, we conclude that after IAN axotomy NPY is produced in trigeminal ganglion neurons and transported in afferent regenerating fibers to the dental pulp. These results add further evidence for a plasticity in peptide transcription in sensory neurons after nerve injury and indicate a trigeminal origin of at least some of the pulpal NPY-IR fibers during nerve regeneration.
Fristad
I
I
Department of Cariology and Endodontics, School of Dentistry, Medical Faculty, University of Bergen, Norway.
Heyeraas
K J
KJ
Kvinnsland
I H
IH
D000367:Age Factors; D000818:Animals; D001369:Axons / Q000737:chemistry* / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D003714:Denervation; D003782:Dental Pulp / Q000294:innervation; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008340:Mandibular Nerve / Q000737:chemistry / Q000166:cytology*; D008963:Molar / Q000294:innervation; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013266:Stilbamidines; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000166:cytology*
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D013266:Stilbamidines; D015740:Calcitonin Gene-Related Peptide