Resistance to ultraviolet light in Pseudomonas syringae: sequence and functional analysis of the plasmid-encoded rulAB genes.
The indigenous plasmids, pPSR1 and pPSR5, were each shown to confer resistance to ultraviolet light (UV) in Pseudomonas syringae (Ps) pv. syringae FF5. The UV-resistance (UVR) determinant was subcloned from a cosmid library of pPSR1, and sequence analysis revealed the presence of two ORFs, designated rulAB which are homologous to the Escherichia coli umuDC mutagenic DNA repair systems and other plasmid-encoded UVR operons. Amino acid (aa) alignments indicated that RulAB are most closely related to the RumAB proteins from plasmid R391, sharing 40.5% and 48.6% aa identity with RumA and RumB, respectively. UV sensitivity assays with the cloned rulAB genes indicated that the expression of UVR in Ps required a functional recA gene.
Sundin
G W
GW
Department of Microbiology and Immunology, University of Illinois-Chicago 60612, USA. sundin@uic.edu
Kidambi
S P
SP
Ullrich
M
M
Bender
C L
CL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000528:radiation effects*; D011836:Radiation Tolerance / Q000235:genetics* / Q000502:physiology; D011938:Rec A Recombinases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C102287:RulA protein, Pseudomonas syringae; C102288:RulB protein, Pseudomonas syringae; D011938:Rec A Recombinases