Ciliary neurotrophic factor receptor alpha mRNA in NB41A3 neuroblastoma cells: regulation by cAMP.
Ciliary neurotrophic factor (CNTF) affects the in vitro and in vivo survival and differentiation of several classes of neurons by binding to the CNTF receptor alpha. We examined the possibility that intracellular cAMP can regulate CNTF receptor alpha mRNA levels in two neuronal cell lines that display cAMP-dependent process outgrowth. Dibutyryl cAMP did not affect CNTF receptor alpha mRNA levels in PC12 cells but elicited a dose- and time-dependent increase in NB41A3 cell CNTF receptor alpha mRNA levels. Forskolin similarly increased CNTF receptor alpha mRNA levels in NB41A3 cells. The data suggest that signal transduction mechanisms involving cAMP may 'crosstalk' with CNTF-initiated signal transduction in a cell type-specific manner and that CNTF receptor alpha expression is not generally linked to neuronal process outgrowth.
MacLennan
A J
AJ
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244, USA.
Gaskin
A A
AA
Vinson
E N
EN
Martinez
L C
LC
D000818:Animals; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D005576:Colforsin; D000242:Cyclic AMP