Determination of chlorobutanol in mouse serum, urine and embryos by capillary gas chromatography with electron capture detection.
A sensitive and specific method for the determination of chlorobutanol (1,1,1-trichloro-2-methyl-2-propanol) in mouse serum, urine, and embryos by capillary gas chromatography with electron capture detection is described. For sample preparation n-hexane was used to extract chlorobutanol and the internal standard 2,2,2-trichloroethanol (TCE) from each matrix. Following extraction chromatographic separation of the samples was achieved with a fused-silica capillary column (30 m x 0.25 mm I.D., 0.25 micron film thickness). The method as described has the required sensitivity to quantitate chlorobutanol in individual embryos following administration of a single oral dose of the drug to a pregnant mouse. The limit of detection was 1 pg on column and the detector response was linear from 1 to 100 micrograms/ml for serum, 0.2 to 20 micrograms/ml for urine, and 1 to 10 ng/embryo.
Amiss
T J
TJ
Department of Anatomy, Physiological Sciences and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Smoak
I W
IW
D000818:Animals; D002724:Chlorobutanol / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D004622:Embryo, Mammalian / Q000737:chemistry*; D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D012680:Sensitivity and Specificity
D005023:Ethylene Chlorohydrin; C005849:2,2,2-trichloroethanol; D002724:Chlorobutanol