Changes in chromatin conformation regulate the 5-lipoxygenase gene expression during differentiation of HL60 cells.
The HL60 cell line does not express the 5-lipoxygenase gene prior to differentiation with various agents. In this paper we have shown by DNase I sensitivity that the chromatin conformation of the 5-lipoxygenase gene changes following differentiation of HL60 cells with dimethyl sulfoxide (DMSO). Moreover, run-on analysis suggests that transcription of the 5-lipoxygenase gene is enhanced after differentiation. We proposed that the chromatin conformation represses the expression of the 5-lipoxygenase gene in HL60 cell line and that differentiation of these cells with DMSO changes the chromatin conformation, which allows the expression of the 5-lipoxygenase gene. The HeLa cells which, like the HL60 cells, do not express the 5-lipoxygenase, were insensitive to DNase I treatment. These results suggest that chromatin structure might represent a form of regulation for the 5-lipoxygenase gene.
Ponton
A
A
Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Canada.
Thirion
J P
JP
Sirois
P
P
D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis / Q000235:genetics*; D001665:Binding Sites; D002454:Cell Differentiation; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D015973:Gene Expression Regulation, Leukemic; D006367:HeLa Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology / Q000235:genetics*; D008968:Molecular Conformation; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D002843:Chromatin; D001094:Arachidonate 5-Lipoxygenase