Identification and cellular localization of protein kinase C isoforms in cultures of rat type-1 astrocytes.
We have examined which isoforms of protein kinase C were present in rat brain astrocytes and found that: (1) the total of calcium-independent isoforms was greater than the total of calcium-dependent isoforms; (2) there were differences in the intracellular distribution of different isoforms; and (3) the abundance of total protein kinase C was greater in astrocytes from cortex than astrocytes from diencephalon.
Roisin
M P
MP
INSERM U29, Hôpital de Port-Royal, Paris, France.
Deschepper
C F
CF
D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004027:Diencephalon / Q000166:cytology / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats
D007527:Isoenzymes; D011493:Protein Kinase C; D002118:Calcium