Effect of dietary soybean phospholipid and fats differing in the degree of unsaturation on fatty acid synthesis and oxidation in rat liver.
The activities of enzymes in fatty acid oxidation and synthesis in the liver of rats fed soybean phospholipids and soybean oil corresponding to the dietary levels of 3% fatty acid added to the diets containing a saturated fat (coconut oil) and a polyunsaturated fat (safflower oil) at the amounts corresponding to 12% fatty acid levels were compared. Soybean phospholipid compared with soybean oil added to both coconut and safflower oil diets significantly reduced the activities of enzymes in fatty acid synthesis (fatty and synthetase, glucose-6-phosphate dehydrogenase and malic enzyme). However, there were no significant differences in the activities of enzymes in fatty acid oxidation (carnitine palmitoyltransferase, acyl-CoA dehydrogenase and acyl-CoA oxidase) between the groups of rats fed soybean phospholipid and soybean oil added to coconut and safflower oil diets except for one occasion. Soybean phospholipid compared with soybean oil added to coconut oil diet significantly decreased the concentrations of triacylglycerol, cholesterol and phospholipid in the serum and of triacylglycerol and cholesterol in the liver. However, the dietary phospholipid added to safflower oil diet failed to alter these values. These results suggested that the alteration in the rate of fatty acid synthesis, but not oxidation, in the liver is responsible for the lipid-lowering effect of dietary soybean phospholipid added to a saturated fat diet.
Kabir
Y
Y
Laboratory of Nutrition Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Japan.
Ide
T
T
D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D000074263:Coconut Oil; D004032:Diet; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012450:Safflower Oil / Q000494:pharmacology; D013024:Soybean Oil / Q000008:administration & dosage / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D005227:Fatty Acids; D010743:Phospholipids; D010938:Plant Oils; D014280:Triglycerides; D013024:Soybean Oil; D012450:Safflower Oil; D002784:Cholesterol; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D000074263:Coconut Oil