Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.
Shen
Z
Z
Life Sciences Division, Los Alamos National Laboratories, New Mexico 87545, USA.
Pardington-Purtymun
P E
PE
Comeaux
J C
JC
Moyzis
R K
RK
Chen
D J
DJ
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D051135:Rad51 Recombinase; D051721:Rad52 DNA Repair and Recombination Protein; D025842:SUMO-1 Protein; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013737:Testis / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C497197:RAD52 protein, S cerevisiae; C497194:RAD52 protein, human; C084232:RHP51 protein, S pombe; D012333:RNA, Messenger; D051721:Rad52 DNA Repair and Recombination Protein; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D016159:Tumor Suppressor Protein p53; D044763:Ubiquitin-Conjugating Enzymes; C495151:RAD51 protein, S cerevisiae; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D008025:Ligases; C093855:HUS5 protein, S pombe; D000098843:Ubiquitin-Conjugating Enzyme UBC9