The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators.
The cloning and expression of two new human cDNAs encoding proteins highly related to the far upstream element-binding protein (FBP) are described. FBP, FBP2, and FBP3 comprise a family of single-strand DNA- binding proteins that possess all of the general features of more conventional transcription factors. The FBPs each bind sequence specifically to only one strand of the far upstream element (FUSE; originally identified upstream of c-myc), and each possesses potent activation domains when fused to the GAL4 DNA-binding domain and assayed by transient transfection. Typical of transcription factors, the proteins are most highly related in their central, DNA-binding domains, but extensive homology is also shared within the tyrosine-rich, carboxyl-terminal activation domains. Comparison with GenBank sequences revealed a fourth FBP family member encoded by Caenorhabditis elegans chromosome III, illustrating the high degree of homology in this evolutionarily ancient and conserved family.
Davis-Smyth
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Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA. levens@helix.nih.gov
Duncan
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Zheng
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Michelotti
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Levens
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009697:Nucleolus Organizer Region; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D035463:Pol1 Transcription Initiation Complex Proteins; D016601:RNA-Binding Proteins; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C105789:KHSRP protein, human; D010940:Plant Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C086367:fbp3 protein, Petunia x hybrida; C063844:transcription factor UBF