Decreased serum antioxidant activity of hemodialysis patients demonstrated by methylguanidine synthesis and microsomal lipid peroxidation.
This study aims to raise the possibility of methylguanidine, a peroxidative product of creatinine, as a measure of the peroxidative state. As a known standard, we measured the inhibitory effect of uremic serum on the NADPH-dependent microsomal lipid peroxidation. This is an established method for evaluating the peroxidative state and is compared to the effect of uremic serum on methylguanidine synthesis. The study shows decreased serum antioxidant activity in hemodialysis patients by both methods, though there is no correlation between them. These results support the use of methylguanidine as a peroxidative marker and suggest a difference in the reactive oxygen species involved in the reactions of methylguanidine synthesis and microsomal lipid peroxidation.
Nagase
S
S
Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Japan.
Aoyagi
K
K
Hirayama
A
A
Gotoh
M
M
Ueda
A
A
Tomida
C
C
Kikuchi
H
H
Takemura
K
K
Koyama
A
A
D000328:Adult; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003404:Creatinine / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008760:Methylguanidine / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar; D006435:Renal Dialysis; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014511:Uremia / Q000097:blood
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; D008760:Methylguanidine; D003404:Creatinine