Human BST-1 expressed on myeloid cells functions as a receptor molecule.
We have previously identified and cloned BST-1 as a molecule which is overexpressed on the bone marrow stromal cell lines derived from patients with rheumatoid arthritis and which has the ability to support the pre-B cell growth. BST-1 is a glycosylphosphatidylinositol-anchored ectoenzyme having ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities. In this report, we demonstrate that human BST-1 was expressed on monocytes, granulocytes in the peripheral blood of healthy donors, and macrophages matured in vitro. Cross-linking of BST-1 with a polyclonal anti-BST-1 antibody induced tyrosine phosphorylation of a 130-kDa protein (p130) in the human myeloid cell lines U937 and THP-1. Cross-linking of BST-1 overexpressed on a transfectant induced tyrosine phosphorylation of p130, dephosphorylation of the 100-kDa protein, and growth inhibition. These results suggest that BST-1 can deliver signals into cells and function as a receptor.
Okuyama
Y
Y
Department of Molecular Oncology, Osaka University Medical School, Japan.
Ishihara
K
K
Kimura
N
N
Hirata
Y
Y
Sato
K
K
Itoh
M
M
Ok
L B
LB
Hirano
T
T
D036541:ADP-ribosyl Cyclase; D000328:Adult; D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics*; D016466:CHO Cells; D002455:Cell Division / Q000276:immunology; D006224:Cricetinae; D005260:Female; D058851:GPI-Linked Proteins; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014162:Transfection; D014443:Tyrosine / Q000378:metabolism
D015703:Antigens, CD; D000954:Antigens, Surface; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014443:Tyrosine; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2