Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin.
Internalin, a surface protein essential for entry of Listeria monocytogenes EGD into epithelial cells, was used as an antigen to raise nine monoclonal antibodies. These monoclonal antibodies recognized seven distinct epitopes which were located in three different regions of the protein. Three of them inhibited internalin-mediated entry and recognized the amino-terminal leucine-rich repeat region of the protein, suggesting that this region is essential for entry.
Mengaud
J
J
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
Lecuit
M
M
Lebrun
M
M
Nato
F
F
Mazie
J C
JC
Cossart
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D015820:Cadherins / Q000096:biosynthesis / Q000276:immunology; D002460:Cell Line; D004269:DNA, Bacterial / Q000235:genetics; D018604:Epitope Mapping; D006801:Humans; D007930:Leucine / Q000276:immunology; D008089:Listeria monocytogenes / Q000187:drug effects*; D008088:Listeriosis / Q000517:prevention & control*; D012091:Repetitive Sequences, Nucleic Acid / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015820:Cadherins; D004269:DNA, Bacterial; C069761:internalin protein, Bacteria; D007930:Leucine