Determination of monensin in high-moisture cattle rations by liquid chromatography with postcolumn derivatization.
An existing liquid chromatographic method using postcolumn derivatization has been used extensively to quantitate monensin in animal feeds. Because of the relatively high moisture content of many cattle feed rations, some modifications were made to this method. Several sample-processing steps were evaluated to determine optimum sample-processing procedure. The sample weight/sample diluent ratio was modified, and method linearity was validated for the lower monensin concentrations anticipated in high-moisture cattle rations. The accuracy and precision of data generated at these lower concentrations were also determined. Because of the high moisture content of these rations, data analysis for this method required correction of feed potency for loss on drying. With these modifications, monensin can be accurately determined in high-moisture cattle rations.
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Division of Eli Lilly and Co., Greenfield, IN 46140-0708, USA.
Roth
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Cleveland
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Moran
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Coleman
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D000821:Animal Feed / Q000592:standards; D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry; D002417:Cattle; D002853:Chromatography, Liquid; D000432:Methanol / Q000737:chemistry; D008985:Monensin / Q000032:analysis*; D010316:Particle Size; D012015:Reference Standards; D015203:Reproducibility of Results; D013464:Sulfuric Acids / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001547:Benzaldehydes; D013464:Sulfuric Acids; D014867:Water; D008985:Monensin; C100058:vanillin; C033158:sulfuric acid; D000432:Methanol