A model-independent, nonlinear extrapolation procedure for the characterization of protein folding energetics from solvent-denaturation data.
We have characterized the guanidine-induced denaturation of hen egg white lysozyme within the 30-75 degrees C temperature range on the basis of equilibrium fluorescence measurements, unfolding assays, kinetic fluorescence measurements, and differential scanning calorimetry. Analysis of the guanidine denaturation profiles according to the linear extrapolation method yields values for the denaturation Gibbs energy which are about 15 kJ/mol lower than those derived from differential scanning calorimetry. Our results strongly suggest that this discrepancy is not due to deviations from the two-state denaturation mechanism. We propose a new method for the determination of denaturation Gibbs energies from solvent-denaturation data (the constant-delta G extrapolation procedure). It employs several solvent-denaturation profiles (obtained at different temperatures) to generate the protein stability curve at zero denaturant concentration within the -8 to 8 kJ/mol delta G range. The method is model-independent and provides a practical, nonlinear alternative to the commonly employed linear extrapolation procedure. The application of the constant-delta G method to our data suggests that the guanidine-concentration dependence of the denaturation Gibbs energy is approximately linear over an extended concentration range but, also, that strong deviations from linearity may occur at low guanidine concentrations. We tentatively attribute these deviations to the abrupt change of the contribution to protein stability that arises from pairwise charge-charge electrostatic interactions. This contribution may be positive, negative, or close to zero, depending on the pH value and the charge distribution on the native protein surface [Yang, A.-S., & Honig, B. (1993) J. Mol. Biol. 231, 459-474], which may help to explain why disparate effects have been found when studying protein denaturation at low guanidine concentrations. Kinetic m values for lysozyme denaturation depend on temperature, in a manner which appears consistent with Hammond behavior.
Ibarra-Molero
B
B
Departamento de Quimica Fisica (Facultad de Ciencias), Instituto de Biotecnologia, Granada, Spain.
Sanchez-Ruiz
J M
JM
D002151:Calorimetry; D004563:Electrochemistry; D019791:Guanidine; D006146:Guanidines / Q000737:chemistry; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding; D012997:Solvents; D013816:Thermodynamics
D006146:Guanidines; D012997:Solvents; D009113:Muramidase; D019791:Guanidine