Intracellular glutathione as a possible direct blocker of HIV type 1 reverse transcription.
In AIDS patients, chronic inflammation and elevated levels of cytokines seem to be associated with reduced levels of glutathione (GSH). GSH has been proposed to inhibit the activation of NF-kB, which results in the inhibition of HIV-1 replication. Here, we show the evidence that GSH and N-acetylcysteine, but not L-cysteine or dithiothreitol, could inhibit the reverse transcriptase (RT) process of HIV-1. Such inhibition was not observed with the RT of murine leukemia virus.
Kameoka
M
M
Section of Serology, Hokkaido University, Sapporo, Japan.
Okada
Y
Y
Tobiume
M
M
Kimura
T
T
Ikuta
K
K
D000111:Acetylcysteine / Q000494:pharmacology*; D001224:Aspartic Acid / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D006801:Humans; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D001224:Aspartic Acid; D054303:HIV Reverse Transcriptase; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol; D000111:Acetylcysteine