title: Immobilizing proteins on the surface of yeast cells.
abstract: Yeast has a rigid cell wall comprising an outer layer of glycoproteins and an internal skeletal layer of glucan; heterologous proteins can be targeted to the glycoprotein layer and become covalently linked to the glucan skeleton. Yeast is a eukaryote that has 'generally regarded as safe' (GRAS) status, and is easy to cultivate, so it seems ideally suited for applications including the manufacture of recyclable, immobilized, biocatalysts, whole-cell vaccines, the presentation of peptide or antibody libraries, and the presentation of adhesion or metal-binding proteins.
authors.affiliation: Department of Molecular Cell Biology, University of Amsterdam, Netherlands.
authors.forename: M P
authors.initials: MP
authors.lastname: Schreuder
authors.forename: A T
authors.initials: AT
authors.lastname: Mooren
authors.forename: H Y
authors.initials: HY
authors.lastname: Toschka
authors.forename: C T
authors.initials: CT
authors.lastname: Verrips
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
mesh_terms: D000284:Administration, Oral; D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000639:trends; D002462:Cell Membrane / Q000378:metabolism*; D004800:Enzymes, Immobilized; D015870:Gene Expression; D037241:Mannose-Binding Lectins; D000072235:Mating Factor; D008565:Membrane Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014612:Vaccines / Q000008:administration & dosage / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D004800:Enzymes, Immobilized; C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014612:Vaccines; D000072235:Mating Factor