Dynamic changes of cathepsins B and L expression in the monkey hippocampus after transient ischemia.
Enzymatic activities, expressions, and the immunohistochemical localization of lysosomal cystein proteases, cathepsins B and L, were analyzed in the monkey hippocampus after transient ischemia to clarify the mechanism of delayed cornu Ammonis (CA)-1 neuronal death. By enzymatic assay, the activity of cathepsin B increased in CA-1, 24 h after the ischemic insult, while that of cathepsin L decreased. On Western blotting, the protein contents of both cathepsins B and L increased immediately after ischemia. By immunohistochemistry, cathepsins B and L were stained as coarse granules in the perikarya of control CA-1 neurons, but in postischemic CA-1 neurons they were released from lysosome granules. In contrast, in CA-2 and the remaining sectors, enzymatic activities increased after ischemia, and immunoreactivities of cathepsins B and L increased only within lysosome granules. These results suggest that cathepsins B and L may play an important role in the breakdown of certain cell proteins in the postischemic CA-1 neurons.
Kohda
Y
Y
Department of Neurosurgery, Kanazawa University School of Medicine, Japan.
Yamashima
T
T
Sakuda
K
K
Yamashita
J
J
Ueno
T
T
Kominami
E
E
Yoshioka
T
T
D000818:Animals; D015153:Blotting, Western; D002401:Cathepsin B / Q000096:biosynthesis* / Q000378:metabolism; D056668:Cathepsin L; D002403:Cathepsins / Q000096:biosynthesis* / Q000378:metabolism; D016923:Cell Death; D003546:Cysteine Endopeptidases; D018891:Dentate Gyrus / Q000201:enzymology; D010450:Endopeptidases; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000473:pathology; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D008251:Macaca; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D015424:Reperfusion; D013379:Substrate Specificity
D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L