The potential synergistic effect of calcium channel blockers and alpha-tocopherol on gastric mucosal injury induced by ischaemia-reperfusion.
RESULTS
Acute oral administration of various doses of alpha-tocopherol (100, 600, 1200 mg/kg by mouth) produced a dose dependent and significant protection of mucosal injury induced by ischaemia-reperfusion injury in experimental animals. In addition, intraperitoneal administration of calcium channel blockers, nifedipine (0.5, 1, 3 mg/kg), diltiazem (1.25, 2.5 mg/kg) and verapamil (1.25, 2.5, 5, 10 mg/kg) protected gastric mucosa in ischaemia-reperfusion. alpha-Tocopherol (100 mg/kg by mouth), when given in combination with either nifedipine (0.5 mg/kg intraperitoneally), diltiazem (1.25 mg/kg intraperitoneally) or verapamil (1.25 mg/kg intraperitoneally), significantly reduced gastric mucosal injury. The protective effect of calcium channel blockers and the antioxidant agent was synergistic.


CONCLUSIONS
The results showed that calcium channel blockers potentiate the protective effect of the antioxidant activity of alpha-tocopherol on gastric mucosal injury induced by ischaemia-reperfusion in experimental animals.
al-Dohayan
A D
AD
Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
al-Tuwaijri
A S
AS
D000284:Administration, Oral; D000818:Animals; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000627:therapeutic use*; D004110:Diltiazem / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000293:injuries; D007274:Injections, Intraperitoneal; D008297:Male; D009543:Nifedipine / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury; D014700:Verapamil / Q000008:administration & dosage / Q000627:therapeutic use*; D014810:Vitamin E / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
D002121:Calcium Channel Blockers; D014810:Vitamin E; D014700:Verapamil; D004110:Diltiazem; D009543:Nifedipine