Highly efficient protein separations in high-performance capillary electrophoresis, using hydrophilic coatings on polysiloxane-bonded columns.
Three methods for preparing hydrophilic coatings on polysiloxane bonded CElect H-type capillary electrophoresis columns have been shown. The polyalkylsiloxane-bonded phase is the first coating layer on the capillary surface, and nonionic surfactant, hydrophilic polymer, or polymer surfactant, adsorbed onto this first later through hydrophobic interactions, forms the second coating layer. The resultant capillary surfaces are inert, hydrophilic, and suitable for highly efficient protein separations. The effectiveness and applicability of these capillary surface modification methods were tested for the separations of a variety of proteins over a wide range of buffer pH values under different capillary electrophoretic operations modes.
Huang
M
M
Supelco, Inc., Bellefonte, PA 16823, USA.
Mitchell
D
D
Bigelow
M
M
D002021:Buffers; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000077423:Polidocanol; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis; D012833:Siloxanes
D002021:Buffers; D007202:Indicators and Reagents; D011506:Proteins; D012833:Siloxanes; D000077423:Polidocanol; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols