title: Inositol 1,4,5-trisphosphate-dependent oscillations of luminal [Ca2+] in permeabilized HSY cells.
abstract: Oscillations in intracellular Ca2+ concentration ([Ca2+]i) are thought to play an important role in phosphoinositide-linked Ca2+ signaling events. We demonstrate corresponding inositol 1,4, 5-trisphosphate (IP3)-dependent oscillations of Ca2+ concentration within the lumen of the IP3-sensitive stores ([Ca2+]L) of saponin-permeabilized HSY cells by monitoring [Ca2+]L with the fluorescent Ca2+ indicator Mag-fura-2. The associated openings and closings of the IP3-sensitive Ca2+ release channel were detected via quenching of Mag-fura-2 fluorescence due to the entry of Mn2+, a Ca2+ surrogate, into the stores. Evidence for complimentary Ca2+ oscillations at the external surface of the stores was provided by the membrane-bound Ca2+ probe Calcium Green C18. The permeabilization of saponin-treated HSY cells to macromolecules was confirmed by demonstrating that permeabilized cells readily took up and lost (t1/2 approximately 46 s) a fluorescently tagged 70-kDa dextran. Our results impose a number of constraints on possible mechanisms for [Ca2+]i oscillations. In addition, they support recent proposals that [Ca2+]i oscillations arise directly from the (biphasic) effects of Ca2+ itself on IP3-sensitive Ca2+ channel activity.
authors.affiliation: Clinical Investigations and Patient Care Branch, NIDR, National Institutes of Health, Bethesda Maryland 20892, USA. rjturner@nih.gov
authors.forename: A
authors.initials: A
authors.lastname: Tanimura
authors.forename: R J
authors.initials: RJ
authors.lastname: Turner
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002451:Cell Compartmentation; D002460:Cell Line; D002463:Cell Membrane Permeability; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D015640:Ion Channel Gating; D008345:Manganese / Q000378:metabolism; D010507:Periodicity; D012469:Salivary Glands / Q000166:cytology; D015398:Signal Transduction
chemical_list: D015220:Calcium Channels; D008345:Manganese; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium