The transcriptional coactivators p300 and CBP are histone acetyltransferases.
p300/CBP is a transcriptional adaptor that integrates signals from many sequence-specific activators via direct interactions. Various cellular and viral factors target p300/CBP to modulate transcription and/or cell cycle progression. One such factor, the cellular p300/CBP associated factor (PCAF), possesses intrinsic histone acetyltransferase activity. Here, we demonstrate that p300/CBP is not only a transcriptional adaptor but also a histone acetyltransferase. p300/CBP represents a novel class of acetyltransferases in that it does not have the conserved motif found among various other acetyltransferases. p300/CBP acetylates all four core histones in nucleosomes. These observations suggest that p300/CBP acetylates nucleosomes in concert with PCAF.
Ogryzko
V V
VV
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753, USA.
Schiltz
R L
RL
Russanova
V
V
Howard
B H
BH
Nakatani
Y
Y
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000256:Adenoviridae / Q000201:enzymology; D000595:Amino Acid Sequence; D050882:CREB-Binding Protein; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D009707:Nucleosomes / Q000737:chemistry / Q000201:enzymology; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015534:Trans-Activators; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism
D006657:Histones; D009687:Nuclear Proteins; D009707:Nucleosomes; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins; D000123:Acetyltransferases; D050882:CREB-Binding Protein; D008239:Lysine