Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO).
Electroporation is one of the most common methods used transform mammalian cells with plasmids. This method is versatile and can be adapted to meet the requirements of many cell lines. However, sometimes the efficiency of this method is low. We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with a beta-Gal expression plasmid in a medium containing DMSO (1.25%) during, and for 24 h after the pulse. In all these cells a dramatic (up to 8-fold) increase in transfection efficiency occurred after this treatment. This method opens up the possibility of using electroporation even in cells which are difficult to transfect.
Melkonyan
H
H
Institute of Experimental Dermatology, University of Münster, Germany.
Sorg
C
C
Klempt
M
M
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D018274:Electroporation; D018922:HL-60 Cells; D006801:Humans; D008322:Mammals; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase; D004121:Dimethyl Sulfoxide