Phenotypic markers for selection of nematode-resistant sheep.
Nematode-resistance of an animal can be defined as an enhanced natural ability, relative to its peers, to both prevent establishment of larval nematodes and evict any that do establish. These parameters are not measurable in a practical sense and consequently nematode-resistance has usually been defined in terms of low faecal nematode egg counts (FEC). Studies in New Zealand and Australia have demonstrated that nematode-resistance, as measured by FEC, has a heritability of about 0.3 in Romney and Merino sheep. However as a selection trait FEC has practical limitations and its use may incur production penalties through withholding drench treatment for prolonged periods or from a need for artificial challenge. FEC is influenced by the level and composition of a natural nematode challenge and especially the expression of the immune response. Thus immunological parameters which reflect the underlying genetic resistance could potentially be used as phenotypic markers. Ideally, a useful phenotypic marker would be easy to sample and its assay would be inexpensive and able to be automated, in addition to being strongly correlated with nematode-resistance. Results from several New Zealand trials have indicated that antibody levels (particularly IgG1) to excretory/secretory antigens of L3 nematodes such as Trichostrongylus colubriformis may meet these criteria. Levels of antibody against L3 antigens are also independent of on-farm drenching strategies. Blood eosinophil count has also been considered for use as a selection parameter but a high degree of sample variability reduces its potential. Other immunological parameters associated with nematode-resistance which have potential as phenotypic markers include serum nematode-specific IgE and products of mucosal mast cells such as proteinases. It is likely that as the critical immune responses of sheep to nematodes become more clearly defined, new immunological parameters with potential for use as phenotypic markers will be found. The definition of these immune responses will also assist in the identification and characterization of genetic markers.
Douch
P G
PG
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. douchp@agresearch.cri.nz
Green
R S
RS
Morris
C A
CA
McEewan
J C
JC
Windon
R G
RG
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005819:Genetic Markers; D007111:Immunity, Cellular; D007113:Immunity, Innate / Q000235:genetics; D009348:Nematoda / Q000276:immunology; D009349:Nematode Infections / Q000276:immunology / Q000662:veterinary*; D010641:Phenotype; D012641:Selection, Genetic; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000909:Antibodies, Helminth; D005819:Genetic Markers