Establishment and characterization of a human tongue cancer xenograft line in nude mice.
We established a human tongue cancer (well-differentiated squamous cell carcinoma) xenograft line, LK-1, in nude mice. LK-1 showed logarithmic growth from 5 to 7 weeks after transplantation, and the take rate for 25 generations was 95.0%. We confirmed the expression of cytokeratins 1, 5, 7, 10, 14, 16, 17, 18 and 19, and type I, III, IV and V collagens by electrophoretical and immunohistochemical analyses. Although the amount of type I, IV and V collagens increased gradually from 5 weeks after transplantation, the distribution of type IV collagen was often discontinuous in the cancer basement membrane. From these data we concluded that LK-1 is an excellent model for the investigation of the cell biology of well-differentiated oral squamous cell carcinoma, and for examining the effects of clinical therapies for this disease.
Tamura
I
I
Department of Biochemistry, Osaka Dental University, Japan.
Sakaki
T
T
Shirasu
R
R
Wato
M
M
Tanaka
A
A
Aoki
S
S
Sakaki
T
T
D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology*; D002454:Cell Differentiation; D003094:Collagen / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D009368:Neoplasm Transplantation; D014062:Tongue Neoplasms / Q000737:chemistry* / Q000473:pathology*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D007633:Keratins; D003094:Collagen