title: Histamine-releasing activity in supernatants of mononuclear cells: contribution of monocyte chemotactic protein-1 activity compared with IgE-dependent activity.
abstract: BACKGROUND
  Supernatants of cultured human mononuclear cells contain factors that induce histamine release from basophils. Some of the histamine-releasing factors present are IgE-independent, but an IgE-dependent form has also been described. The IgE that does respond to the IgE-dependent histamine-releasing factor was defined as IgE+.
  
  
  OBJECTIVE
  This study was conducted to analyze the contribution of chemokines, such as monocyte chemotactic protein-1 (IgE-independent), to improve the detection of IgE-dependent histamine-releasing factor.
  
  
  METHODS
  Supernatants were prepared from mononuclear cells of three subjects. Monocyte chemotactic protein-1 content and histamine-releasing activity (HRA) were measured. Depletion of chemokine activity was carried out with heparin-Sepharose (Pharmacia, Uppasala, Sweden).
  
  
  RESULTS
  Replacing IgE- on basophils with IgE+ serum decreased the correlation between monocyte chemotactic protein-1 and histamine release (rho = 0.80, n = 280 vs p = 0.12, n = 18; p for difference between p values; < 0.05). After depletion of chemokines in three supernatants derived from mononuclear cells, IgE-dependent HRA was still present.
  
  
  CONCLUSIONS
  We conclude that in supernatants derived from mononuclear cells, the IgE-independent HRA masks the IgE-dependent HRA. The latter can be more clearly detected after depletion of chemokine activity with heparin-Sepharose.
authors.affiliation: Department of Allergy, Netherlands Red Cross Blood Transfusion Service, The Netherlands.
authors.forename: S G
authors.initials: SG
authors.lastname: Pasmans
authors.forename: M
authors.initials: M
authors.lastname: Aalbers
authors.forename: M R
authors.initials: MR
authors.lastname: Daha
authors.forename: E F
authors.initials: EF
authors.lastname: Knol
authors.forename: H M
authors.initials: HM
authors.lastname: Jansen
authors.forename: R C
authors.initials: RC
authors.lastname: Aalberse
mesh_terms: D000818:Animals; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism; D001685:Biological Factors / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000494:pharmacology*; D054410:Chemokine CCL7; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines; D006493:Heparin / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D011817:Rabbits; D011852:Radioallergosorbent Test; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D001685:Biological Factors; C515450:CCL7 protein, human; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D017077:Culture Media, Conditioned; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D006493:Heparin