The influence of serum for spreading of tumor cells on synthetic glycolipid films.
Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.
Sato
T
T
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Endo
M
M
Okahata
Y
Y
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology*; D016895:Culture Media, Serum-Free; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism*; D006367:HeLa Cells / Q000166:cytology / Q000473:pathology; D006801:Humans; D007785:Lactose / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D008320:Maltose / Q000378:metabolism; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D006017:Glycolipids; D010713:Phosphatidylcholines; D008320:Maltose; D005947:Glucose; D007785:Lactose; D005690:Galactose