title: [Effective binding of alpha-bungarotoxin with the solubilized alpha-subunit of Torpedo californica acetylcholine receptor].
abstract: alpha-Subunit of the Torpedo californica nicotinic acetylcholine receptor was isolated by preparative SDS-PAGE followed by reversed-phase HPLC on a C4 column in an acetonitrile-isopropanol gradient in water. After removal of the organic solvents and solubilization in beta-octylglucoside, the purified alpha-subunit binds alpha-bungarotoxin with high affinity (Kd 28 nM).
authors.forename: Iu N
authors.initials: IuN
authors.lastname: Utkin
authors.forename: M
authors.initials: M
authors.lastname: Mund
authors.forename: F
authors.initials: F
authors.lastname: Hucho
authors.forename: V I
authors.initials: VI
authors.lastname: Tsetlin
mesh_terms: D000818:Animals; D002038:Bungarotoxins / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility; D014101:Torpedo
chemical_list: D002038:Bungarotoxins; D011978:Receptors, Nicotinic