Expression of Vpre-B3 (8HS-20) molecules by alternative RNA processing.
In pre-B cells, mu chains are expressed in association with "surrogate' L chains encoded by the lambda 5 and Vpre-B1 genes. In addition to their association with lambda 5 and Vpre-B1, mu chains in pre-B cells are associated with the products of the Vpre-B3 gene (formerly designated 8HS-20), which display a distinct association with mu chains and biochemical properties in terms of mol. wt, pI value and glycosylation. However, the mechanism of the generation of Vpre-B3 isoforms has been unknown. The present study indicates that the Vpre-B3 gene transcript underwent alternative RNA processing in normal B cells, in a pre-B cell lymphoma and in a mature B cell lymphoma, WEHI 231, that was transfected with the Vpre-B3 genomic clone. Vpre-B3 isoforms were expressed in a WEHI 231 cell line transfected with the Vpre-B3 genomic clone, comparable in biochemical nature to those expressed in a pre-B cell lymphoma. In contrast, expression of one of the isoforms was missing in a cell line transfected with the Vpre-B3 cDNA clone. These results suggest that Vpre-B3 isoforms with distinct biochemical characteristics are derived from alternatively processed Vpre-B3 mRNA.
Hagiwara
S
S
Department of Immunology, National Institute of Health of Japan, Tokyo, Japan.
Tsunetsugu-Yokota
Y
Y
Kimoto
H
H
Takemori
T
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D007147:Immunoglobulin Light Chains / Q000235:genetics; D054444:Immunoglobulin Light Chains, Surrogate; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D007147:Immunoglobulin Light Chains; D054444:Immunoglobulin Light Chains, Surrogate; D007135:Immunoglobulin Variable Region; D007148:Immunoglobulin mu-Chains; D008562:Membrane Glycoproteins; D012333:RNA, Messenger