Immobilized liposome chromatography of drugs on capillary continuous beds for model analysis of drug-membrane interactions.
Liposomes were immobilized in capillary continuous beds with covalently linked C4 or C8 alkyl ligands for chromatographic analysis of drug interaction with phospholipid bilayers, as reflected by drug retention volumes and calculated differences in interaction free energies. This procedure is a high-resolution micro-scale version of immobilized liposome chromatography for prediction of diffusion of drugs across biological membranes. The logarithm of the specific capacity factors of several structurally unrelated drugs showed a linear correlation with the logarithm of known apparent drug permeabilities through Caco-2 epithelial cell monolayers. The latter values are used for prediction of absorption of orally administered drug doses.
Zhang
Y
Y
Department of Biochemistry, Uppsala University, Sweden.
Zeng
C M
CM
Li
Y M
YM
Hjertén
S
S
Lundahl
P
P
D008051:Lipid Bilayers; D008081:Liposomes; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D013816:Thermodynamics
D008051:Lipid Bilayers; D008081:Liposomes; D004364:Pharmaceutical Preparations