title: Epitopes of Bordetella pertussis lipopolysaccharides as potential markers for typing of isolates with monoclonal antibodies.
abstract: Three hybridomas (P1P3, D7 and 60.5) producing monoclonal antibodies (mAbs) against Bordetella pertussis lipopolysaccharide (LPS) were established. All reacted with the LPS from a typical, vaccine strain of B. pertussis (1414), but not with that of a variant strain (A100). Two of these mAbs (P1P3 and 60.5) cross-reacted with a B. bronchiseptica LPS; only one (P1P3) reacted with a B. parapertussis LPS. ELISA reactivities with intact LPSs, and defined partial structures covalently linked to bovine serum albumin, were compared. mAb 60.5 bound to the terminal region of a distal trisaccharide consisting of N-acetylated amino sugars. D7 reacted with a substructure which can be modified in the B. parapertussis and B. bronchiseptica LPSs by addition of a polymeric O-chain. P1P3 bound to a nonacetylated glucosamine substituted with L-glycero-D-manno-heptose, present in the 'core' of the B. pertussis LPS. These mAbs may be useful for rapid typing of Bordetella in clinical isolates.
authors.affiliation: Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
authors.forename: Karine Le
authors.initials: KL
authors.lastname: Blay
authors.affiliation: Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
authors.affiliation: Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Blanchard
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
authors.affiliation: Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Chaby
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry; D015373:Bacterial Typing Techniques; D015415:Biomarkers; D001884:Bordetella / Q000276:immunology; D001886:Bordetella pertussis / Q000145:classification* / Q000276:immunology*; D002240:Carbohydrate Sequence; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006825:Hybridomas / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015415:Biomarkers; D000939:Epitopes; D008070:Lipopolysaccharides; D012710:Serum Albumin, Bovine