title: Bellini duct carcinoma. A clinical and in vitro study.
abstract: OBJECTIVE
  Bellini duct carcinoma (BDC) is a rare and highly aggressive renal tumor whose histogenesis is still a matter of debate although a putative origin from collecting ducts has been proposed.
  
  
  METHODS
  A primary tumor cell culture was obtained from a BDC of a 57-year-old man who presented with a mass of the right kidney. The patient died from disease progression 18 months after diagnosis. The light and ultrastructural features were consistent with previous reports on BDC. The expression of low (Ker 18) and high (Ker 5, Ker 8, Ker 10) molecular weight keratins was studied.
  
  
  RESULTS
  The BDC tumor cells displayed strong positivity for keratins, 5, 8 and 18 but did not react with the anti-keratin 10 antibody. Northern blot analysis of total mRNA revealed expression of the c-erbB-1 oncogene unlike two conventional clear cell carcinomas of the kidney used as control. Cytogenetic analysis revealed an aneuploid karyotype: 53,XY,del(1)(p34),+iso(1q),+iso(5p),+4,+7,+8,-14,del(16)(q22). No submicroscopic deletion on p14-21 and p26 regions of the short arm of chromosome 3 was detected on Southern blot analysis.
  
  
  CONCLUSIONS
  The absence of structural changes in the short arm of chromosome 3 (usually present in hereditary and sporadic renal cell carcinomas) in the presence of chromosomal abnormalities observed in malignant lesions of urothelial origin confers to BDC a unique genetic profile among papillary tumors of the kidney.
authors.affiliation: Department of Surgery, University of Rome Tor Vergata, Italy.
authors.forename: A O
authors.initials: AO
authors.lastname: Cavazzana
authors.forename: T
authors.initials: T
authors.lastname: Prayer-Galetti
authors.forename: R
authors.initials: R
authors.lastname: Tirabosco
authors.forename: M C
authors.initials: MC
authors.lastname: Macciomei
authors.forename: M
authors.initials: M
authors.lastname: Stella
authors.forename: L
authors.initials: L
authors.lastname: Lania
authors.forename: P
authors.initials: P
authors.lastname: Cannada-Bartoli
authors.forename: L G
authors.initials: LG
authors.lastname: Spagnoli
authors.forename: G
authors.initials: G
authors.lastname: Passerini-Glazel
authors.forename: F
authors.initials: F
authors.lastname: Pagano
mesh_terms: D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D066246:ErbB Receptors / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D007685:Kidney Tubules, Collecting; D008297:Male; D008875:Middle Aged; D009006:Monosomy; D014314:Trisomy
chemical_list: D007633:Keratins; D066246:ErbB Receptors