Structural and functional analyses of APG5, a gene involved in autophagy in yeast.
The APG5 gene of Saccharomyces cerevisiae was cloned from a yeast genomic library by complementation of autophagy defective phenotype of apg5-1 mutant. Structural analysis of the obtained genomic fragment showed that the APG5 gene encodes a novel hydrophilic protein of 294 amino-acid residues without apparent structural similarities to other proteins in the database. To examine its function, a null allele for APG5 (delta apg5) was constructed and introduced into yeast. delta apg5 cells germinated and grew normally in nutrient-rich condition, however, their viability reduced significantly upon the nutrient starvation. They were also shown to be defective in autophagy: they could not sequester autophagic bodies in the vacuole under nitrogen-starvation conditions. These phenotypes are identical to those found in the apg5-1 mutant. The lack of apparent phenotype in rich medium suggests that APG5 function is required only under nutrient starvation condition, however, Northern blot analysis showed that its expression levels remained unchanged after nutrient depletion.
Kametaka
S
S
Department of Biology, Graduate School of Arts and Sciences, University of Tokyo, Japan.
Matsuura
A
A
Wada
Y
Y
Ohsumi
Y
Y
D000595:Amino Acid Sequence; D000071187:Autophagy-Related Protein 5; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D008247:Lysosomes / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases
C102268:ATG5 protein, S cerevisiae; D000071187:Autophagy-Related Protein 5; D004271:DNA, Fungal; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases