Characteristic localization of gp130 (the signal-transducing receptor component used in common for IL-6/IL-11/CNTF/LIF/OSM) in the rat brain.
The localization of gp130, the signal transducing receptor component used in common for interleukin (IL)-6, IL-11, ciliary neurotrophic factor (CNTF), LIF and OSM, in the rat brain was demonstrated by immunohistochemistry using an antibody specific to gp130. The gp130 immunoreactivity was observed in both glial and neuronal cells. Two distinct neuronal staining patterns were observed. The first showed neuropil staining, observed mainly in telencephalic structures including the hippocampus, cerebral cortex and caudate-putamen. The second pattern was observed on the cytoplasmic membrane of neuronal somata and was found primarily in the lower brainstem, in the large neurons of the reticular formation, and in spinal and cranial motor neurons. Electron-microscopic analysis revealed that both types of gp130 immunoreactivity were primarily associated with the cytoplasmic membrane and were not localized exactly at synaptic sites. Further, gp130 immunoreactivity was also observed in the oligodendrocytes and subependymal zone. These widespread but characteristic patterns of gp130 immunoreactivity overlap well with those of IL-6 receptor and CNTF alpha chains, suggesting a role of cytokines and growth factors such as IL-6 and CNTF via gp130 in certain specific regions of the brain.
Watanabe
D
D
Department of Neuroanatomy, Osaka University Medical School, Japan.
Yoshimura
R
R
Khalil
M
M
Yoshida
K
K
Kishimoto
T
T
Taga
T
T
Kiyama
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D001923:Brain Chemistry / Q000502:physiology*; D050822:Cytokine Receptor gp130; D006131:Growth Inhibitors; D007150:Immunohistochemistry; D053703:Interleukin-11 Receptor alpha Subunit; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D020801:Receptor, Ciliary Neurotrophic Factor; D018121:Receptors, Cytokine / Q000032:analysis; D018123:Receptors, Interleukin / Q000032:analysis*; D053702:Receptors, Interleukin-11; D019947:Receptors, Interleukin-6; D017475:Receptors, Nerve Growth Factor / Q000032:analysis; D053606:Receptors, OSM-LIF; D053677:Receptors, Oncostatin M; D015398:Signal Transduction / Q000502:physiology*
D015703:Antigens, CD; D006131:Growth Inhibitors; C508430:Il11ra1 protein, rat; C494201:Il6st protein, rat; D053703:Interleukin-11 Receptor alpha Subunit; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507842:Lifr protein, rat; D008222:Lymphokines; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D020801:Receptor, Ciliary Neurotrophic Factor; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D019947:Receptors, Interleukin-6; D017475:Receptors, Nerve Growth Factor; D053606:Receptors, OSM-LIF; D053677:Receptors, Oncostatin M; D050822:Cytokine Receptor gp130