title: [1-13C]glucose metabolism in brain cells: isotopomer analysis of glutamine from cerebellar astrocytes and glutamate from granule cells.
abstract: We analyzed the glutamine isotopomers released into the extracellular medium by cerebellar astrocytes incubated with [1-13C]glucose. We developed a mathematical model of the tricarboxylic acid (TCA) cycle to determine the relative flux of molecules through the anaplerotic versus oxidative pathways and the relative pyruvate carboxylase versus pyruvate dehydrogenase activities. As glutamine C2 and C3 exhibited unequivalent enrichments, we examined the possibility of: (1) both the entry of the label into the TCA cycle from pyruvate and the conversion of the oxaloacetate into citrate before equilibration with fumarate, and (2) the occurrence of an orientation-conserved transfer of the symmetrical 4-carbon intermediates. The best fit required partial cycling between oxaloacetate and fumarate, whereas the occurrence of any orientation-conserved transfer was rejected. On the other hand, the analysis of glutamate isotopomers from perchloric acid extracts of granule cells incubated with [1-13C]glucose indicated that total cycling of oxaloacetate into fumarate occurred in these cells.
authors.affiliation: Unité de Résonance Magétique des Systèmes Biologiques, UMR 5536, Bordeaux, France.
authors.forename: M
authors.initials: M
authors.lastname: Merle
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Villégier
authors.forename: P
authors.initials: P
authors.lastname: Canioni
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002247:Carbon Isotopes; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism*; D051381:Rats
chemical_list: D002247:Carbon Isotopes; D005973:Glutamine; D018698:Glutamic Acid; D005947:Glucose