title: An in vivo pathway for disulfide bond isomerization in Escherichia coli.
abstract: Biochemical studies have shown that the periplasmic protein disulfide oxidoreductase DsbC can isomerize aberrant disulfide bonds. Here we present the first evidence for an in vivo role of DsbC in disulfide bond isomerization. Furthermore, our data suggest that the enzymes DsbA and DsbC play distinct roles in the cell in disulfide bond formation and isomerization, respectively. We have shown that mutants in dsbC display a defect in disulfide bond formation specific for proteins with multiple disulfide bonds. The defect can be complemented by the addition of reduced dithiothreitol to the medium, suggesting that absence of DsbC results in accumulation of misoxidized proteins. Mutations in the dipZ and trxA genes have similar phenotypes. We propose that DipZ, a cytoplasmic membrane protein with a thioredoxin-like domain, and thioredoxin, the product of the trxA gene, are components of a pathway for maintaining DsbC active as a protein disulfide bond isomerase.
authors.affiliation: Department of Microbilogy and Molecular Genetics, harvard Medical School, Boston, MA 02115, USA.
authors.forename: A
authors.initials: A
authors.lastname: Rietsch
authors.forename: D
authors.initials: D
authors.lastname: Belin
authors.forename: N
authors.initials: N
authors.lastname: Martin
authors.forename: J
authors.initials: J
authors.lastname: Beckwith
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002465:Cell Movement; D004220:Disulfides / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D005838:Genotype; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D016296:Mutagenesis; D010957:Plasmids; D019704:Protein Disulfide-Isomerases; D011994:Recombinant Proteins / Q000378:metabolism; D013489:Suppression, Genetic
chemical_list: D004220:Disulfides; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D007535:Isomerases; D019704:Protein Disulfide-Isomerases