Production of eicosanoids derived from 20:4n-6 and 20:5n-3 in primary cultures of turbot (Scophthalmus maximus) brain astrocytes in response to platelet activating factor, substance P and interleukin-1 beta.
Primary cultures of turbot (Scophthalmus maximus) brain astroglial cells established in medium containing fetal bovine serum contain increased proportions of 18:1(n-9), total (n-9) and (n-6) polyunsaturated fatty acids (PUFA) and greatly reduced (n-3) PUFA in comparison with turbot brain. Supplementation with a mixture of 5 microM eicosapentaenoic [20:5(n-3)] and 25 microM docosahexaenoic [22:6(n-3)] acids for 4 days significantly increased the percentages of these acids in total cellular lipid of turbot astrocytes and restored the (n-3) PUFA composition of the cells to that found in turbot brain. The production of prostaglandins (PG) E and F of the 2- and 3-series and leukotrienes (LT) C4 and C5 in response to various agonists was determined in PUFA-supplemented astrocytes. Calcium ionophore A23187, platelet activating factor and substance P stimulated the production of both PGF and PGE. Interleukin-1 beta significantly stimulated the production of PGF only. There were differences between the agonists in their effects on the relative levels of 2- and 3-series PGs produced. Only very low amounts of LTC were produced by the turbot astrocytes, with only substance P showing a minor stimulatory effect.
Tocher
D R
DR
NERC Unit of Aquatic Biochemistry, Department of Biological and Molecular Sciences, University of Stirling, Scotland, UK. drt1@uk.ac.stir.forth
Bell
J G
JG
Sargent
J R
JR
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D015777:Eicosanoids / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D005412:Flatfishes / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D015289:Leukotrienes / Q000096:biosynthesis; D010972:Platelet Activating Factor / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis; D013373:Substance P / Q000494:pharmacology
D003470:Culture Media; D015777:Eicosanoids; D005231:Fatty Acids, Unsaturated; D007375:Interleukin-1; D015289:Leukotrienes; D010972:Platelet Activating Factor; D011453:Prostaglandins; D013373:Substance P