Regulation of spiramycin synthesis in Streptomyces ambofaciens: effects of glucose and inorganic phosphate.
The production of the 16-membered macrolide antibiotic, spiramycin, in Streptomyces ambofaciens is inhibited by glucose, 2-deoxyglucose and inorganic phosphate. The role of intracellular ATP content and phosphorylated metabolites as common regulating signals of both glucose and phosphate inhibitory effects is discussed. Two enzymatic targets of the effect of phosphate on spiramycin biosynthesis were studied. Valine dehydrogenase, the first enzyme of valine catabolism (supplier of aglycone spiramycin precursors), and alkaline phosphatase, which cleaves phosphorylated intermediates, were repressed in the presence of excess phosphate.
Lounès
A
A
Laboratoire de Microbiologie Industrielle et Alimentaire, ENSAIA, Institut National Polytechnique de Lorraine, Vandoeuvre, France.
Lebrihi
A
A
Benslimane
C
C
Lefebvre
G
G
Germain
P
P
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D003912:Dextrins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D015572:Spiramycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D003912:Dextrins; D010710:Phosphates; D012329:RNA, Bacterial; D015572:Spiramycin; D000255:Adenosine Triphosphate; D000594:Amino Acid Oxidoreductases; C057545:valine dehydrogenase (NAD+); D000469:Alkaline Phosphatase; D005947:Glucose