An immunohistochemical study of the pathogenesis of virulent viscerotropic Newcastle disease in chickens.
Ten specific pathogen-free cockerels aged 8-12 months were infected by ocular instillation of 2 x 10(7) embryo-lethal doses 50% of a virulent viscerotropic strain of Newcastle disease virus (Fontana 1083 strain). The birds were killed at 24-h intervals after infection and tissues were collected for histological and immunohistochemical evaluation. The infection ran an acute course, severe clinical disease occurring within 72 h of infection. By gross and histological examination, the most severe damage detected was found to occur in lymphoid areas in a number of tissues. The greatest amounts of viral antigen detected occurred in the proventriculus, small intestine, spleen, thymus and eyelid. Immunohistochemical labelling was confined to large mononuclear cells.
Ojok
L
L
Makerere University, Kampala, Uganda.
Brown
C
C
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002645:Chickens; D005143:Eyelids / Q000473:pathology / Q000821:virology; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000473:pathology / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008221:Lymphoid Tissue / Q000473:pathology* / Q000821:virology; D008264:Macrophages / Q000276:immunology; D009521:Newcastle Disease / Q000276:immunology / Q000473:pathology* / Q000821:virology; D011531:Proventriculus / Q000473:pathology / Q000821:virology; D013154:Spleen / Q000473:pathology / Q000821:virology; D013950:Thymus Gland / Q000473:pathology / Q000821:virology; D013997:Time Factors; D014132:Trachea / Q000473:pathology / Q000821:virology
D000956:Antigens, Viral