Corticotropin-releasing factor modulates basal and stress-induced excitatory amino acid release in the locus coeruleus of conscious rats.
The in vivo interactions between corticotropin-releasing factor (CRF) and excitatory amino acid (EAA) release in the locus coeruleus (LC) were studied. Superfusion of the LC with CRF (0.1 microM) led to a prolonged increase in the release rate of aspartate and, to a lesser extent, of glutamate. The CRF antagonist alpha-helical CRF9-41 (1 microM) had no effect on basal EAA release but abolished the enhanced aspartate and glutamate release induced by noise stress (95 dB). Tail pinch-induced EAA release was not influenced by alpha-helical CRF9-41. Results demonstrate a facilitatory action of CRF on in vivo EAA release in the LC. Furthermore, modulation by CRF of stress-induced EAA release in the LC depends on the nature of stress.
Singewald
N
N
Department of Pharmacology and Toxicology, University of Innsbruck, Austria.
Zhou
G Y
GY
Chen
F
F
Philippu
A
A
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D008125:Locus Coeruleus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009622:Noise / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D018846:Excitatory Amino Acids; D013779:Tetrodotoxin; D003346:Corticotropin-Releasing Hormone