A novel method for production of monoclonal antibodies. Evaluation and expectation of the rat lymph node method in cell and molecular biology.
A newly developed method using rat lymph nodes provided us with substantial amounts of high quality monoclonal antibodies. The method has characteristic features, a single injection of an emulsified antigen via the rat's hind footpads is sufficient for immunization. Enlarged medial iliac lymph nodes can be used for cell fusion to produce hybridomas 2 weeks and later after the antigen injection. The production frequency of target hybridomas is about 10 times higher than that of a conventional spleen method. Our current knowledge about the production of monoclonal antipeptide antibodies and monoclonal nephritogenic autoantibodies by the method is described.
Sado
Y
Y
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
Okigaki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000096:biosynthesis*; D000941:Antigens / Q000008:administration & dosage / Q000235:genetics; D003094:Collagen / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000235:genetics; D005260:Female; D005921:Glomerulonephritis / Q000209:etiology / Q000473:pathology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007158:Immunologic Techniques; D007668:Kidney / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology*; D008969:Molecular Sequence Data; D051381:Rats; D011921:Rats, Inbred WKY
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D003094:Collagen