title: Immunogenic and protective response in mice immunized with a purified, inactivated, Dengue-2 virus vaccine prototype made in fetal rhesus lung cells.
abstract: The feasibility of a purified, inactivated vaccine (PIV) against dengue type 2 (DEN-2) virus was explored. Dengue-2 virus strain 16681 was used for producing a monotypic PIV. Virus adapted to fetal rhesus lung (FRhL-2) cells was harvested from roller bottle culture supernatant fluids, concentrated, and purified on sucrose gradients. Analysis of purified virus preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting showed primarily envelope (E) and premembrane (prM) antigens. These preparations had a purity, estimated from silver-stained gels, of approximately 70%, and a yield, based on recovery of virus and viral antigen, of 10-20%. The purified virus was inactivated with 0.05% formalin at 22 degrees C, or alternatively, with 7 mRads from a 60Co source. Vaccinated mice developed high titers of anti-DEN-2 virus neutralizing antibody and were partially protected from virus challenge. These results warrant further testing and development of PIVs for the other DEN virus serotypes.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, District of Columbia, USA.
authors.forename: R
authors.initials: R
authors.lastname: Putnak
authors.forename: K
authors.initials: K
authors.lastname: Cassidy
authors.forename: N
authors.initials: N
authors.lastname: Conforti
authors.forename: R
authors.initials: R
authors.lastname: Lee
authors.forename: D
authors.initials: D
authors.lastname: Sollazzo
authors.forename: T
authors.initials: T
authors.lastname: Truong
authors.forename: E
authors.initials: E
authors.lastname: Ing
authors.forename: D
authors.initials: D
authors.lastname: Dubois
authors.forename: J
authors.initials: J
authors.lastname: Sparkuhl
authors.forename: W
authors.initials: W
authors.lastname: Gastle
authors.forename: C
authors.initials: C
authors.lastname: Hoke
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D002478:Cells, Cultured / Q000821:virology; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control*; D003716:Dengue Virus / Q000276:immunology* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008168:Lung / Q000166:cytology; D008253:Macaca mulatta; D051379:Mice; D008813:Mice, Inbred ICR; D009500:Neutralization Tests; D015164:Vaccines, Inactivated / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins