Expression of peptides encoded by exons in cloned mammalian DNA.
New synthetic approaches, such as combinatorial chemistry, provide a rich source of potential drug candidates. At the same time, the human genome initiative and other large-scale sequencing projects provide a large number of novel drug targets. However, the functional analysis of thousands of new genes remains a major challenge for the future. A systematic strategy for genome-wide functional analysis of genes could employ the fact that at least some modules in multi-domain proteins are encoded in individual exons. Exon amplification provides information about coding regions of most genes that is independent of their transcriptional status; exon amplification from entire mammalian genomes has been demonstrated. Here, we describe the development of an exon-trap system, lambdaGEE (for genomic exon expression), that couples exon amplification with the expression of exon-encoded peptides.
Kreissig
S
S
Deutsches Krebforschungszentrum, Heidelberg, Germany.
Schüddekopf
K
K
Dear
N
N
Boehm
T
T
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D005784:Gene Amplification; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008322:Mammals; D010455:Peptides / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D051820:TCF Transcription Factors; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D010455:Peptides; D011994:Recombinant Proteins; D051820:TCF Transcription Factors; C544912:TCF7L1 protein, human; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors; D004247:DNA