title: The neuronal stress response: nuclear translocation of heat shock proteins as an indicator of hyperthermic stress.
abstract: Two characteristic features of the heat shock response, (i) induction of hsp70 protein and (ii) nuclear translocation of constitutive hsc70 and stress-inducible hsp70 protein, were utilized as markers of cellular stress in the rabbit brain. Following a physiologically relevant increase in body temperature of 2.7 +/- .3 degrees C, nonneuronal cell types, such as ependymal cells and oligodendrocytes, undergo a stress response as assayed by the above criteria. In contrast, several neuronal cell populations required an increased degree of hyperthermic stress (3.4 +/- .2 degrees C) before exhibiting nuclear translocation of constitutive hsc70 protein. Induction of hsp70 protein was not observed in these neuronal cells at either temperature. The present results suggest that certain neurons in the rabbit brain are buffered against induction of the heat shock response, perhaps due to their high constitutive levels of hsc70 protein.
authors.affiliation: Department of Zoology, University of Toronto, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Manzerra
authors.forename: I R
authors.initials: IR
authors.lastname: Brown
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D001831:Body Temperature; D001921:Brain / Q000166:cytology / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D004805:Ependyma / Q000502:physiology; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D006979:Hyperthermia, Induced; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009836:Oligodendroglia / Q000502:physiology; D011817:Rabbits; D012016:Reference Values; D013312:Stress, Physiological
chemical_list: D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins