Increased expression of keratinocyte interleukin-8 in human contact eczematous reactions to heavy metals.
Heavy metal-induced contact eczematous human skin reactions to cobalt chloride and mercuric chloride were investigated for immunoreactivity to interleukin-8 (IL-8), by using an indirect immunoperoxidase staining technique. There was suprabasal epidermal staining for IL-8, with a decrease in the vicinity of areas with parakeratotic epithelium. However, in the immediate vicinity of a vesicular formation, intense staining of some apically situated keratinocytes was found. In addition, increased immunoreactivity over the acrosyringial area compared with the surrounding epidermis was obtained. These findings indicate an increased synthesis of keratinocyte IL-8 in contact eczematous skin.
Chen
L
L
Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
Nordlind
K
K
Lidén
S
S
Sticherling
M
M
D003035:Cobalt; D003877:Dermatitis, Contact / Q000503:physiopathology*; D004485:Eczema / Q000503:physiopathology*; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008627:Mercuric Chloride; D012882:Skin Tests
D016209:Interleukin-8; D003035:Cobalt; D008627:Mercuric Chloride; C018021:cobaltous chloride