Survival of Cryptosporidium muris (strain MCR) oocysts under cryopreservation.
We have successfully maintained Cryptosporidium muris by cryopreservation. Oocysts were suspended in distilled water, stored at -20 degrees C for 24 hrs, and then cryopreserved at -70 degrees C. Cryopreserved specimens were slowly thawed at 5 degrees C. Oocysts, which had been cryopreserved for 15 months without cryoprotective agents, retained their infectivity by the mouse titration method. Oocysts stored at 5 degrees C in 2.5% potassium dichromate failed to retain their infectivity beyond 6.5 months.
Rhee
J K
JK
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju, Korea.
Park
B K
BK
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003458:Cryptosporidium / Q000472:pathogenicity; D051379:Mice