Determination of noscapine, hexylresorcinol and anethole in cough lozenges by liquid chromatography.
A liquid chromatographic method was developed for the simultaneous separation and determination of noscapine hydrochloride, hexylresorcinol and anethole in cough lozenges. Analysis was performed on a phenyl column with phosphate buffer- acetonitrile as mobile phase and the separated components were detected at 282 mm. Recoveries obtained for the analytes were of 94.6% for noscapine hydrochloride, 99.1% for hexylresorcinol and 96.3% for anethole. The values of the relative standard deviation were 0.8% for noscapine hydrochloride, 1.5% for hexylresorcinol and 1.1% for anethole. The analytical method was validated and a system suitability test was accomplished for the chromatographic method.
Lucangioli
S
S
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Química Analitica y Fisicoquímica, Argentina.
Fernández Otero
G
G
Rodríguez
V
V
Carducci
C N
CN
D000781:Anethole Trithione / Q000032:analysis*; D000996:Antitussive Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D006604:Hexylresorcinol / Q000032:analysis*; D007202:Indicators and Reagents; D009665:Noscapine / Q000032:analysis*; D013607:Tablets
D000996:Antitussive Agents; D007202:Indicators and Reagents; D013607:Tablets; D009665:Noscapine; D000781:Anethole Trithione; D006604:Hexylresorcinol