Defects in the development of branchial nerves and ganglia induced by in vitro exposure of mouse embryos to mercuric chloride.
The embryotoxic and dysmorphogenic effects of mercuric chloride (HgCl2) have been studied in mouse embryos cultured in vitro. In addition, the alterations induced in the developing branchial nerves and ganglia were analyzed. Mouse embryos with 6-8 pairs of somites were exposed for 26 hr to increasing concentrations (0, 12.5, 25, 50 microM) of HgCl2. After this period, a first set of embryos was removed and a second set of embryos transferred to culture medium without HgCl2 and remained in culture for an additional 22 hr. Both sets of embryos were examined for (1) survival, (2) presence of external dysmorphogenesis, (3) growth, and (4) differentiation. Dose-related alterations of these parameters were observed. The main target was the cephalic neural tube (mainly the forebrain), but several other systems were also affected (e.g., the turning of the embryos, the optic system). The 48-hr cultured embryos were immunostained using a monoclonal antineurofilament antibody to visualize defects in the development of branchial nerves and ganglia. HgCl2 induced a pronounced retardation in the differentiation of ganglion/nerve V and a slight retardation in the differentiation of ganglia/nerves VII and IX. The ganglia/nerves VIII and X were not retarded. In addition, hight percentages of abnormalities of ganglion/nerve V and fusions between ganglia/nerves IX and X were observed in these embryos. Disorganized fibers between ganglia/nerves VII-VIII and IX and between ganglia/nerves IX and X were also more frequently observed. At the highest concentration, asymmetric defects were induced by HgCl2 with a more pronounced effect observed on the right side of the embryos. These results demonstrate the usefulness of this approach in evaluating the susceptibility of the developing branchial nerves to the adverse effects of developmental toxicants.
van Maele-Fabry
G
G
Laboratory of Developmental Genetics, Catholic University of Louvain, Louvain-la-Neuve, Belgium.
Gofflot
F
F
Picard
J J
JJ
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001934:Branchial Region / Q000294:innervation*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000002:abnormalities / Q000187:drug effects*; D005154:Facial Nerve / Q000187:drug effects / Q000196:embryology; D005260:Female; D005724:Ganglia / Q000187:drug effects; D005930:Glossopharyngeal Nerve / Q000187:drug effects / Q000196:embryology; D008627:Mercuric Chloride / Q000633:toxicity*; D051379:Mice; D011247:Pregnancy; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000196:embryology; D014276:Trigeminal Nerve / Q000187:drug effects / Q000196:embryology; D014630:Vagus Nerve / Q000187:drug effects / Q000196:embryology; D000159:Vestibulocochlear Nerve / Q000187:drug effects / Q000196:embryology
D008627:Mercuric Chloride