Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex.
Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied. 51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.
Hiort
C
C
Department of Chemistry, Syracuse University, NY 13244-4100, USA.
Goodisman
J
J
Dabrowiak
J C
JC
D004270:DNA, Circular / Q000187:drug effects* / Q000378:metabolism; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology; D008027:Light; D010545:Peroxides / Q000494:pharmacology; D017968:Vanadium Compounds / Q000494:pharmacology*
D004270:DNA, Circular; D007328:Insulin; D010545:Peroxides; D017968:Vanadium Compounds