Isolation of T cell receptor gamma/delta chain constant genes from minke whale.
The polymerase chain reaction using oligonucleotides based on consensus sequences from other species allowed us to amplify minke whale (Balaenoptera acutorostrata) T cell receptor (TcR) gamma and delta constant genes. Two types of Cgamma clones were obtained which only differ by one mismatch. These minke whale Cgamma clones showed 83% nucleotide and 70% amino acid sequence similarity to the corresponding region of the human TRGC genes. The minke whale Cdelta sequences were all identical, and showed 86% nucleotide and 77.8% amino acid sequence similarity to the human TRDC gene. The whale Cgamma and Cdelta clones were used as probes for Southern blot analysis. The data confirmed that there is a unique Cdelta gene in minke whale. Two different Cgamma genes were detected, one of them also cross-hybridizing with a human Cgamma probe, suggesting that the Cgamma locus in minke whale consists of at least two different constant genes.
Zhang
X M
XM
Laboratoire d'ImmunoGenetique Moleculaire, LIGM, Institute de Genetique Moleculaire, Universites Montpellier, France.
Huck
S
S
Dariavach
P
P
Spilliaert
R
R
Arnason
A
A
Soua
Z
Z
Lefranc
G
G
Lefranc
M P
MP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014907:Whales / Q000235:genetics* / Q000276:immunology*
D017260:Receptor-CD3 Complex, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta