title: Renal organic anion secretion: evidence for dopaminergic and adrenergic regulation.
abstract: To examine possible regulatory control of renal proximal tubule organic anion secretion, winter flounder (Pleuronectes americanus) proximal tubule primary cultures were mounted in Ussing chambers. Unidirectional fluxes of [2,4-(14)C]dichlorophenoxyacetic acid were determined under short-circuited conditions. Phorbol 12-myristate 13-acetate (1 microM) caused a significant (P < 0.01) inhibition of net 2,4-dichlorophenoxyacetic acid secretion. Preincubation with staurosporine (1 microM) blocked the phorbol 12-myristate 13-acetate-induced decrease in secretion. Neither forskolin (10 microM) nor W-7 (20 microM) had any effect on net transport. Elevation of intracellular calcium activity with either A-23187 or thapsigargin produced a slight, transient decrease in transport. Addition of dopamine (1 microM) to the peritubular side, but not the luminal side, caused a significant (P < 0.01) decrease in net secretion. Both the alpha-adrenergic agonist oxymetazoline (10 microM) and depletion of intracellular Na+ transiently, but significantly (P < 0.05), increased net transport. The data indicate that renal organic anion excretion may be regulated through dopaminergic inhibition and alpha-adrenergic stimulation of net transepithelial secretion.
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Halpin
authors.forename: J L
authors.initials: JL
authors.lastname: Renfro
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism; D018663:Adrenergic Agents / Q000494:pharmacology*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D000838:Anions / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D004789:Enzyme Activation; D005432:Flounder; D007425:Intracellular Membranes / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D010109:Oxymetazoline / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D018663:Adrenergic Agents; D000316:Adrenergic alpha-Agonists; D000838:Anions; D015084:2,4-Dichlorophenoxyacetic Acid; D010109:Oxymetazoline; D011494:Protein Kinases; D011339:Probenecid; D002118:Calcium; D004298:Dopamine