Identification and mapping of a putative bombesin receptor gene on human chromosome 17q21.3+.
A mouse bombesin receptor cDNA was used as a probe to screen a human P1 genomic library. Clone HBR1 was isolated and used to localize a putative human bombesin receptor gene (HBRKS) on human chromosome 17q21.3 by fluorescent in situ hybridization (FISH). HBRKS was identified and mapped by polymerase chain reaction (PCR) amplification from a Yeast artificial chromosome (YAC) contig spanning 17q21-q23. In addition, a few candidate genes were found by exon-trapping from HBR1.
Kelavkar
U
U
Department of Biological Sciences, Clark Atlanta University, GA, USA.
Abel
K
K
Miller
D
D
Murtagh
J
J
Shah
K
K
D000818:Animals; D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D015698:Genomic Library; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D018004:Receptors, Bombesin / Q000235:genetics*
D018004:Receptors, Bombesin