title: No relation between angiotensin-converting enzyme gene polymorphism and dermal response to bradykinin in healthy subjects.
abstract: 1. The dermal wheal response to bradykinin is increased by drugs which inhibit angiotensin-converting enzyme, and thus provides a measure of angiotensin-converting enzyme activity at the tissue level. An insertion/deletion polymorphism of the angiotensin-converting enzyme gene predicts serum angiotensin-converting enzyme activity, but its relation to angiotensin-converting enzyme activity in tissue is unclear. 2. The relations between angiotensin-converting enzyme genotype and wheal responses to intradermal bradykinin were studied in 105 healthy subjects: 30 of genotype DD, 51 of genotype ID and 24 of genotype II. Dermal wheal area was measured by digitized planimetry and the angiotensin-converting enzyme genotype by polymerase chain reaction. 3. Bradykinin produced significant linear log dose-wheal area responses. The potency of bradykinin by parallel line bioassay did not differ significantly between the genotypes; the potency in the II subjects relative to DD subjects was 1.25 (95% confidence interval: 0.83-1.88). 4. Although the angiotensin-converting enzyme gene polymorphism is a consistent and powerful predictor of serum angiotensin-converting enzyme activity, it does not appear to predict tissue angiotensin-converting enzyme activity as measured by dermal responses to bradykinin.
authors.affiliation: University Department of Medicine and Pharmacology, Royal Hallamshire Hospital Sheffield, U.K.
authors.forename: I G
authors.initials: IG
authors.lastname: Chadwick
authors.forename: M
authors.initials: M
authors.lastname: Kraskiewicz
authors.forename: W W
authors.initials: WW
authors.lastname: Yeo
authors.forename: K S
authors.initials: KS
authors.lastname: Higgins
authors.forename: P R
authors.initials: PR
authors.lastname: Jackson
authors.forename: L E
authors.initials: LE
authors.lastname: Ramsay
mesh_terms: D000328:Adult; D001920:Bradykinin / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005838:Genotype; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics* / Q000378:metabolism
chemical_list: D006632:Histamine; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin