title: Purification of protein C a natural anticoagulant, from human plasma by affinity chromatography with Convanavalin A.
abstract: We have developed a rapid, efficient, and inexpensive method to purify protein C from plasma using Concanavalin A conjugated to Sepharose. With this method we have obtained protein C purified to homogeneity, as measured in polyacrylamide gels. We discuss each of the steps of our method and give the results of the purification.
authors.affiliation: Biochemistry and Immunology Unit, Technological Institute of Oaxaca, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Pérez-Campos
authors.forename: F
authors.initials: F
authors.lastname: Córdoba
authors.forename: E
authors.initials: E
authors.lastname: Pérez-Ortega
authors.forename: M
authors.initials: M
authors.lastname: Martínez
authors.forename: E
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000925:Anticoagulants / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011486:Protein C / Q000302:isolation & purification*
chemical_list: D000925:Anticoagulants; D011486:Protein C; D003208:Concanavalin A