title: Intracellular Ca2+ mobilization in cultured human mucosal microvascular endothelial cells from the nasal inferior turbinate.
abstract: Human mucosal microvascular endothelial cells (HMMECs) were isolated and cultured from the mucosa of nasal inferior turbinates. These cells were identified by human factor-VIII antigen and UEA-I. We also investigated alterations in intracellular calcium concentration [Ca2+]i, in HMMECs using the Ca(2+)-sensitive dye fura-2. Depolarization by high K+ (150 mM) induced no changes in [Ca2+]i in HMMECs, thereby indicating the lack of voltage-dependent Ca2+ channels in these cells. Extracellular ATP increased [Ca2+]i in HMMECs both in the presence and absence of extracellular Ca2+. These results suggest that ATP may induce a release of Ca2+ from intracellular stores in HMMECs. Adenosine induced no changes in [Ca2+]i in HMMECs. It is thus likely that HMMECs obtained from the nasal inferior turbinate may carry P2-purinoceptors but not P1-purinoceptors.
authors.affiliation: Department of Otorhinolaryngology, Kansai Medical University, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: N
authors.initials: N
authors.lastname: Kubo
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes; D016257:Fura-2; D006651:Histocytochemistry; D006801:Humans; D008856:Microscopy, Fluorescence; D009297:Nasal Mucosa / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000378:metabolism; D014420:Turbinates / Q000098:blood supply / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D011983:Receptors, Purinergic; D002118:Calcium; D016257:Fura-2