title: Enzymatic synthesis of oleamide (cis-9, 10-octadecenoamide), an endogenous sleep-inducing lipid, by rat brain microsomes.
abstract: The enzymatic synthesis of a novel sleep-inducing lipid, oleamide (cis-9,10-octadecenoamide), was studied using rat brain subcellular fractions as enzyme sources. We found that oleamide was formed from oleic acid and ammonia on incubation with a brain homogenate. The enzyme activity catalyzing the formation of oleamide from oleic acid and ammonia was highest in the microsomal fraction among the subcellular fractions. Boiled microsomes did not exhibit appreciable enzyme activity. These results strongly suggest that oleamide can be synthesized enzymatically in the brains of stimulated animals.
authors.affiliation: Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugiura
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Kodaka
authors.forename: T
authors.initials: T
authors.lastname: Tonegawa
authors.forename: S
authors.initials: S
authors.lastname: Nakane
authors.forename: A
authors.initials: A
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Ishima
authors.forename: K
authors.initials: K
authors.lastname: Waku
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003065:Coenzyme A / Q000494:pharmacology; D005973:Glutamine / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D019301:Oleic Acid / Q000378:metabolism; D009829:Oleic Acids / Q000096:biosynthesis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D009829:Oleic Acids; D005973:Glutamine; D019301:Oleic Acid; D000641:Ammonia; C029407:oleylamide; D000255:Adenosine Triphosphate; D003065:Coenzyme A