Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.
We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.
Hampson
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IN
CRC Department of Experimental Haematology, Paterson Institute of Cancer Research, Christie Hospital, Manchester, UK.
Hampson
L
L
Pinkoski
M
M
Cross
M
M
Heyworth
C M
CM
Bleackley
R C
RC
Atkinson
E
E
Dexter
T M
TM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D006098:Granulocytes; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages; D051379:Mice; D008969:Molecular Sequence Data; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D014162:Transfection
C490400:Serpina3k protein, mouse; D015843:Serpins