The development of cowpea mosaic virus as a potential source of novel vaccines.
Epitopes from human rhinovirus 14 (HRV-14) and human immunodeficiency virus type (HIV-1) have been expressed on the surface of particles of the plant virus, cowpea mosaic virus (CPMV). The chimaeras retain their ability to grow in plants and large quantities of virions can be easily purified. Immunological studies have shown that purified particles have the antigenic properties of the insert, and, in the case of the HIV-1 chimaera, can elicit the production of neutralising antibodies in mice. The chimaera containing the epitope from HRV-14 has been crystallised and the crystals shown to diffract to atomic resolution.
Porta
C
C
Department of Virus Research, John Innes Centre, Norwich, UK.
Spall
V E
VE
Lin
T
T
Johnson
J E
JE
Lomonossoff
G P
GP
D016915:AIDS Vaccines / Q000096:biosynthesis* / Q000276:immunology; D000595:Amino Acid Sequence; D017798:Comovirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000648:ultrastructure; D005822:Genetic Vectors; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000821:virology; D012229:Rhinovirus / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology
D016915:AIDS Vaccines; D015700:HIV Envelope Protein gp41; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C074995:viral protein 1, rhinovirus