title: Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction.
abstract: The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403, USA. fritz@uoneuro.uoregon.edu
authors.forename: A
authors.initials: A
authors.lastname: Fritz
authors.forename: M
authors.initials: M
authors.lastname: Rozowski
authors.forename: C
authors.initials: C
authors.lastname: Walker
authors.forename: M
authors.initials: M
authors.lastname: Westerfield
mesh_terms: D000818:Animals; D017931:DNA Primers; D005720:Gamma Rays; D009154:Mutation / Q000528:radiation effects*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015027:Zebrafish / Q000235:genetics*
chemical_list: D017931:DNA Primers