Determination of Z-butylidenephthalide in plasma by high-performance liquid chromatography.
An HPLC assay is described for the determination of Z-butylidenephthalide (Z-Bdph) in plasma. Plasma samples were cleaned up by extraction with 2% chloroform in n-hexane. Z-Bdph was separated on a normal-phase silica column with a mobile phase of chloroform-n-hexane (1:1). The limit of quantitation with UV detection at 254 nm for Z-Bdph in plasma was 0.01 microgram/ml. The recovery of Z-Bdph by organic solvent extraction of plasma was 99.5%. The intra-day and inter-day coefficients of variation and relative errors for Z-Bdph determination in plasma were both less than 10%. The present method was applied to pharmacokinetic studies of Z-Bdph in plasma after intravenous administration to rabbits.
Ko
W C
WC
Department of Pharmacology, Taipei Medical College, Taiwan.
Yang
J Y
JY
Chen
C M
CM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D010276:Parasympatholytics / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D010796:Phthalic Anhydrides / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D010276:Parasympatholytics; D010796:Phthalic Anhydrides; C026105:butylidenephthalide