Treatment of rats with the peroxisome proliferator ciprofibrate results in increased liver NF-kappaB activity.
Nuclear factor kappaB (NF-kappaB) is an important stress-induced transcription factor in many cell types, including hepatocytes. Previous studies have shown that reactive oxygen species, including hydrogen peroxide, are potent activators of NF-kappaB. Peroxisome proliferators are a group of rodent chemical carcinogens that have been proposed to act by increasing reactive oxygen in the liver. These results led us to consider whether peroxisome proliferators would increase NF-kappaB activity in the liver. Here we demonstrate that rats fed diets containing the peroxisome proliferator ciprofibrate exhibit increased hepatic NF-kappaB DNA-binding activity. This observation suggests that NF-kappaB may contribute, in part, to peroxisome proliferator-mediated changes in the liver.
Li
Y
Y
Graduate Center for Toxicology, University of Kentucky, College of Medicine, Lexington 40536-0084, USA.
Leung
L K
LK
Glauert
H P
HP
Spear
B T
BT
D000818:Animals; D001665:Binding Sites; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004586:Electrophoresis; D058607:Fibric Acids; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008830:Microbodies / Q000187:drug effects* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats
D058607:Fibric Acids; D000960:Hypolipidemic Agents; D016328:NF-kappa B; D002995:Clofibric Acid; D004247:DNA; C019304:ciprofibrate