title: Receptor protein tyrosine kinase DDR is up-regulated by p53 protein.
abstract: We have previously reported on radiation-induction of ptk-3 in rat astrocyte culture [Sakuma et al. (1995) Radiat. Res. 143, 1-7]. Ptk-3 was considered to be a rat version of human DDR (discoidin domain receptor). We cloned and analyzed genomic DNA of the DDR and its promoter region. We discovered that the promoter region contained a consensus sequence of the p53 tumor suppressor binding site. Adenovirus-mediated p53 transfection induced a high level of DDR mRNA in SAOS2 human osteosarcoma cells. These results indicate that DDR is up-regulated by the p53 protein.
authors.affiliation: Department of Neurosurgery, University of Hokkaido School of Medicine, Kita-ku, Sapporo, Japan. ssakuma@med.hokudai.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: H
authors.initials: H
authors.lastname: Saya
authors.forename: M
authors.initials: M
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: J A
authors.initials: JA
authors.lastname: Roth
authors.forename: Y
authors.initials: Y
authors.lastname: Sawamura
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohe
authors.forename: H
authors.initials: H
authors.lastname: Abe
mesh_terms: D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000070818:Discoidin Domain Receptors; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D011975:Receptors, Mitogen / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D011975:Receptors, Mitogen; D016159:Tumor Suppressor Protein p53; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases