Characterization of a human alphoid satellite DNA sequence: potential use in assessing genetic diversity in Indian populations.
Sequence analysis of a human repetitive DNA sequence (pTRF5.6) revealed considerable homology (76%) to the alphoid consensus sequence. Genomic blots of StuI-digested human DNA, hybridized to pTRF5.6, generated a ladder of bands with each band corresponding to oligodeoxyribonucleotide of an approx. 170-bp repeat, indicating a tandemly arrayed organization of this repeat element within the genome. Genomic hybridization analyses of unrelated individuals belonging to various geographical regions of India, using this alphoid satellite prove, revealed polymorphic bands ranging between 2 and 9 kb. Along with an individual-specific band pattern, several isomorphic bands below 2 kb were also evident. There was very little genetic variability between populations, suggestive of low polymorphism at the inter-population level. Our result suggest that alphoid satellite sequence probe can be used in assessing the genetic diversity of various ethnic groups/populations belonging to different geographical regions.
Raina
A
A
Department of Forensic Medicine, All India Institute of Medical Sciences, New Delhi, India.
Sulaiman
I M
IM
Ehtesham
N Z
NZ
Das
P
P
Ali
S
S
Dogra
T D
TD
Hasnain
S E
SE
D001483:Base Sequence; D016172:DNA Fingerprinting; D004276:DNA, Satellite; D014644:Genetic Variation; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite