title: Effect of phagocytosis of bacteria on the uptake of sparfloxacin by human neutrophils.
abstract: The effect of phagocytosis of Escherichia coli, Staphylococcus aureus and Haemophilus influenzae on the intracellular penetration of sparfloxacin into human polymorphonuclear neutrophils (PMN) was evaluated. The intracellular concentration of sparfloxacin was similar in PMN containing intracellular bacteria and in resting PMN [cellular to extracellular ratio (C/ E) > 8.0]. The uptake of sparfloxacin by human PMN significantly decreased during phagocytosis of opsonized H. influenzae but still reached intracellular concentrations five times greater than the extracellular ones. This effect was not observed with E. coli and S. aureus.
authors.affiliation: Department of Microbiology, School of Medicine, University of Seville, Spain.
authors.forename: I
authors.initials: I
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Pascual
authors.forename: S
authors.initials: S
authors.lastname: Ballesta
authors.forename: E J
authors.initials: EJ
authors.lastname: Perea
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood*; D004926:Escherichia coli / Q000187:drug effects; D005069:Evaluation Studies as Topic; D024841:Fluoroquinolones; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006193:Haemophilus influenzae / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology*; D015363:Quinolones / Q000097:blood*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C061363:sparfloxacin