Study of local anaesthetics. Part 133. Determination of diperodon in blood serum in vitro by the HPLC method.
A local anaesthetics diperodon by the action of hydrolazes in blood serum is decomposed. A method for its determination in blood serum in vitro has been worked out. It was found that during the first 2 hours it decomposes rather rapidly. During next 6 hours the rate of decomposition is decreased, and after 8 hours the blood serum contained yet about 33% of not decomposed diperodon. The separation of diperodon has been carried out in the chromatographic system which consists of 70% methanol in mobile phase in water with sodium acetate (6.8 g/l, pH = 7.4) and chemically bounded phase C18. The capacity ratio of diperodon has been 8.1, the limit of determination is 5 micrograms/ml, and the exctraction recovery is 70%.
Cizmárik
J
J
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
Lehotay
J
J
Hromuláková
K
K
D000779:Anesthetics, Local / Q000097:blood*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D009994:Osmolar Concentration; D010880:Piperidines / Q000097:blood*; D011817:Rabbits; D012015:Reference Standards; D019346:Sodium Acetate / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000779:Anesthetics, Local; D010880:Piperidines; D014867:Water; C027013:diperodon; D019346:Sodium Acetate; D000432:Methanol