High-performance liquid chromatographic assay for meropenem in serum.
High-performance liquid chromatographic procedures have been developed for the measurement of meropenem in serum. The separation was performed on an Ultrasphere XL-ODS analytical column (75 x 4.6 min I.D.). The mobile phase consisted of 10.53 mmol/l ammonium acetate-acetonitrile (95:5, v/v) (pH 4). The UV detection was at 298 nm. The quantitation limit both in serum and water was 0.25 micrograms/ml. The method was validated in serum and aqueous solution over the concentration range 0.25-50 micrograms/ml. The extraction recovery from serum spiked with meropenem was 99.7 +/- 3.4%. The intra- and inter-assay coefficients of variation were below 6%. Stored at -80 degrees C for three months at various concentrations in serum and in aqueous solution, meropenem did not reveal any appreciable degradation. After 24 h, it was also stable at 4 degrees C in serum, aqueous solution and supernatant of extraction but not at room temperature. The stability of the drug was also confirmed in serum after repeated freezing-thawing cycles at -80 degrees C on four consecutive days.
Elkhaïli
H
H
Laboratory of Pharmacokinetics, University Hospital Center, Strasbourg, France.
Niedergang
S
S
Pompei
D
D
Linger
L
L
Leveque
D
D
Jehl
F
F
D000818:Animals; D015780:Carbapenems / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007275:Injections, Intravenous; D000077731:Meropenem; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013556:Swine, Miniature; D013696:Temperature; D013845:Thienamycins / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D013997:Time Factors
D015780:Carbapenems; D013845:Thienamycins; D000077731:Meropenem