title: Analysis of Glial-Specific Gene Expression Using in Vitro Transcription Assays
abstract: The in vitro transcription system using extracts from animal tissues is one of the most powerful and convenient ways to investigate transcriptional regulation of tissue-specific genes when appropriate cultured cells are not available. In the present study, we established a protocol for preparing transcription-competent nuclear extracts from mouse brain. Transcription in the extract could mimic in vivo tissue specificity qualitatively, suggesting that this technique is beneficial for studying transcriptional regulation of nervous system-specific genes. By using these methods, we analyzed cis-elements of the glial cell-specific mouse myelin basic protein gene.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba, 263, Japan
authors.initials: Ta
authors.lastname: Tamura
authors.initials: Y
authors.lastname: Konishi
authors.initials: Y
authors.lastname: Makino
authors.initials: K
authors.lastname: Mikoshiba