title: [Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization].
abstract: Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.
authors.forename: N G
authors.initials: NG
authors.lastname: Gurskaia
authors.forename: D A
authors.initials: DA
authors.lastname: Shagin
authors.forename: K A
authors.initials: KA
authors.lastname: Luk'ianov
authors.forename: L L
authors.initials: LL
authors.lastname: Vagner
authors.forename: M S
authors.initials: MS
authors.lastname: Shtutman
authors.forename: E A
authors.initials: EA
authors.lastname: Musatkina
authors.forename: E V
authors.initials: EV
authors.lastname: Moinova
authors.forename: A G
authors.initials: AG
authors.lastname: Tatosian
authors.forename: S A
authors.initials: SA
authors.lastname: Luk'ianov
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000595:Amino Acid Sequence; D066258:Amphiregulin; D000818:Animals; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012377:Rodentia; D017386:Sequence Homology, Amino Acid
chemical_list: C587848:AREG protein, human; D066258:Amphiregulin; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins
vernacular_title: Klonirovanie s pomoshch'iu vychitaiushcheĭ gibridizatsii kDNK gena ha-SDGF iz linii kletok siriĭskogo khomiachka s povyshennym potentsialom metastazirovaniia.