Determination of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats.
A simple method for determination of mimosine and 3,4-dihydroxypyridine (3,4-DHP) in plasma and milk was developed. Milk and plasma, with tyrosine as internal standard, were deproteinized using 9% trichloracetic acid and extracted with diethyl ether. Metabolites were separated by isocratic high-performance liquid chromatography, with 0.02 M orthophosphoric acid (pH 2.5) at 0.5 ml/min and a Hypersil ODS microbore column. Mimosine, 3,4-DHP and tyrosine were detected at 275 nm. The recovery of the mimosine added to the plasma samples was 101.6 +/- 2.3% and 103.3 +/- 1.0% for milk samples. 3,4-DHP recovery for plasma samples was 101.2 +/- 0.9% and for milk samples 100.8 +/- 1.4%. The reproducibility of the method was evaluated by analyzing six plasma samples and six goat milk samples. The analyses yielded relative standard deviations of 2.65 and 2.82%, respectively.
Puchala
R
R
E (Kika) de la Garza Institute for Goat Research, Langston University, OK 73050, USA.
Davis
J J
JJ
Sahlu
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006041:Goats / Q000097:blood / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry*; D008898:Mimosine / Q000032:analysis* / Q000097:blood; D011725:Pyridines / Q000032:analysis* / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D014443:Tyrosine / Q000032:analysis
D011725:Pyridines; D014443:Tyrosine; D008898:Mimosine; C007435:3,4-dihydroxypyridine