title: Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.
abstract: We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.
authors.affiliation: CRC Department of Experimental Haematology, Paterson Institute of Cancer Research, Christie Hospital, Manchester, UK.
authors.forename: I N
authors.initials: IN
authors.lastname: Hampson
authors.forename: L
authors.initials: L
authors.lastname: Hampson
authors.forename: M
authors.initials: M
authors.lastname: Pinkoski
authors.forename: M
authors.initials: M
authors.lastname: Cross
authors.forename: C M
authors.initials: CM
authors.lastname: Heyworth
authors.forename: R C
authors.initials: RC
authors.lastname: Bleackley
authors.forename: E
authors.initials: E
authors.lastname: Atkinson
authors.forename: T M
authors.initials: TM
authors.lastname: Dexter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D006098:Granulocytes; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages; D051379:Mice; D008969:Molecular Sequence Data; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D014162:Transfection
chemical_list: C490400:Serpina3k protein, mouse; D015843:Serpins