Antigenic similarities among biotin-binding proteins.
Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [3H]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner.
Subramanian
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Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Subramanian
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Adiga
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PR
D000818:Animals; D000906:Antibodies; D001360:Avidin / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001710:Biotin / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D000939:Epitopes / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D019809:Streptavidin
D000906:Antibodies; D001426:Bacterial Proteins; D002352:Carrier Proteins; D000939:Epitopes; C017071:biotin-binding proteins; D001360:Avidin; D001710:Biotin; D019809:Streptavidin