A putative pyruvate dehydrogenase alpha subunit gene from Trypanosoma cruzi.
A full-length DNA clone encoding a putative pyruvate dehydrogenase alpha subunit (E1 alpha) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1 alpha obtained from different sources. The highest score is obtained with human E1 alpha: 43,3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1 alpha gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA.
Buscaglia
C A
CA
Instituto de Investigaciones Bioquímicas Fundación Campomar, Buenos Aires, Argentina.
Pollevick
G D
GD
Veloso
C
C
Lorca
M
M
Frasch
A C
AC
Sánchez
D O
DO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D018628:Gene Dosage; D017125:Genes, Protozoan / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D016053:RNA, Protozoan / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics*
D016054:DNA, Protozoan; D011768:Pyruvate Dehydrogenase Complex; D012333:RNA, Messenger; D016053:RNA, Protozoan