Differential effects of intrathecal thyrotropin-releasing hormone (TRH) on perineal reflexes in male rats.
The effects of thyrotropin-releasing hormone (TRH) on the sexual and defecatory reflexes regulated by pudendal motoneurons were investigated. Intrathecal TRH (10 microliters volume; 0.0, 0.01, 1.0 or 100 microM concentration) at lumbosacral spinal segments (L4-S1) in acute preparations produced a dose-dependent increase in external anal sphincter (EAS), but not bulbospongiosus (BS), electromyographic (EMG) activity. Intraspinal (L6) injection of 100 microM TRH (1 microliter/micropipette), significantly increased EAS EMG activity in acute preparations. Electromyographic activity of the BS muscle was unchanged. All doses of intrathecal TRH (10 microliters volume; 0, 10, 50, 100, or 500 microM concentration) in awake animals significantly reduced the proportion of responders to a penile reflex test. Subsequently, all measures of penile reflexes were significantly reduced. Glans tumescence and defecation bouts before or during penile reflex testing were unaffected by intrathecal TRH as were indices of behavioral and motor hyper-reactivity analogous to that produced by serotonin. These data indicate that pudendal motoneurons, in the dorsomedial nucleus, are differentially regulated by neuropeptides present in the lumbosacral spinal cord.
Holmes
G M
GM
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210, USA. holmes.38@osu.edu
Rogers
R C
RC
Bresnahan
J C
JC
Beattie
M S
MS
D001003:Anal Canal / Q000294:innervation*; D000818:Animals; D003672:Defecation / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004576:Electromyography; D008297:Male; D009046:Motor Neurons / Q000502:physiology; D010410:Penile Erection / Q000502:physiology; D010413:Penis / Q000294:innervation*; D051381:Rats; D012018:Reflex / Q000502:physiology*; D012726:Sexual Behavior, Animal / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*; D013973:Thyrotropin-Releasing Hormone / Q000502:physiology*
D013973:Thyrotropin-Releasing Hormone