INMP, a novel intranuclear matrix protein related to the family of intermediate filament-like proteins: molecular cloning and sequence analysis.
An important step in understanding nuclear structure and its function in replication and gene regulation is the cloning and characterisation of nuclear matrix proteins. A full-length cDNA-clone, encoding a novel nuclear matrix protein, was isolated from a (lambda gt11 cDNA library derived from murine macrophages. The antibody used for the screen was raised against a single polypeptide isolated from two-dimensional gel electrophoresis of nuclear matrix preparations. The cDNA encodes a protein of 446 amino acids named INMP for intranuclear matrix protein. INMP displays several salient features, a coiled-coil domain, a leucine zipper, a number of phosphorylation sites and a putative nuclear localisation signal. Sequence homology comparisons indicate that INMP is a unique protein which is evolutionary related to the gene family of intermediate filament-like proteins, especially the nuclear lamins.
Menz
K
K
Genetisches Institut, Justus-Liebig-Universität Giessen, Germany.
Radomski
N
N
Jost
E
E
D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear; D001483:Base Sequence; D002417:Cattle; D002467:Cell Nucleus; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007526:Isoelectric Point; D016350:Leucine Zippers; D008099:Liver / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D010766:Phosphorylation; D010802:Phylogeny; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D034961:Antigens, Nuclear; D018076:DNA, Complementary; D007381:Intermediate Filament Proteins; D009687:Nuclear Proteins