Expression of alpha v and beta 3 integrin chains on murine lymphocytes.
The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. We have generated four mAbs that recognize the murine alpha v beta 3 vitronectin receptor. Biochemical and expression analyses showed that two of the mAbs are specific for the alpha v chain, and two are specific for the beta 3 chain. The mAbs are effective blocking reagents and inhibited cell adhesion to vitronectin, fibrinogen, and fibronectin. Staining analysis revealed expression of alpha v and beta 3 on certain populations of murine thymocytes, splenocytes, and bone marrow cells. The expression of alpha v and beta 3 appeared to be modulated at specific stages of thymocyte development, suggesting a possible function for the alpha v beta 3 vitronectin receptor in T cell development.
Gerber
D J
DJ
Howard Hughes Medical Center, Center for Cancer Research, Cambridge, MA, USA.
Pereira
P
P
Huang
S Y
SY
Pelletier
C
C
Tonegawa
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D005434:Flow Cytometry; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011233:Precipitin Tests; D019030:Receptors, Vitronectin / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D019030:Receptors, Vitronectin