Enteric neurons express Steel factor-lacZ transgene in the murine gastrointestinal tract.
The cells that express Steel factor (SLF) in the gastrointestinal (GI) tract were studied using SLF-lacZ transgenic mice. Expression, detected by beta-galactosidase histochemistry, was evident in cells between the circular and longitudinal muscle layers in the GI tract. Double staining with antibodies specific for the neural markers, PGP 9.5, MAP2 and c-Ret, showed that SLF-lacZ positive cells were enteric neurons. Enteroglia did not express SLF-lacZ. The distribution of expressing cells was complimentary to the expression of c-Kit in myenteric interstitial cells.
Torihashi
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S
Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno 89557, USA.
Yoshida
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H
Nishikawa
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Kunisada
T
T
Sanders
K M
KM
D000818:Animals; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D019089:Stem Cell Factor / Q000235:genetics*; D001616:beta-Galactosidase / Q000032:analysis
D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit; D001616:beta-Galactosidase