Dissociate arachidonic acid release from eicosanoid synthesis in rat peritoneal macrophages is possible using concomitantly eicosapentanoic acid as a phospholipid analogue.
Activated macrophages exposed to the association of eicosapentanoic acid 20:5 n-3 and a synthetic non hydrolysable phospholipid analogue maintained a discrete synthesis of active eicosanoids. 20:4 n-6 split from the internalized 20:4-GPC was accumulated in cells and extracellular fluids. This combination thus represents a novel approach to reduce the 20:4 n-6 cascade.
Bourass
J
J
Laboratoire de Synthèse et d'étude des Substances Naturelles à Activité Biologique, Põle Sciences, La Rochelle, France.
Boucrot
P
P
Letourneux
Y
Y
Gandemer
G
G
Petit
J Y
JY
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D015777:Eicosanoids / Q000096:biosynthesis*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D015777:Eicosanoids; D005230:Fatty Acids, Nonesterified; D007476:Ionophores; D010743:Phospholipids; D016718:Arachidonic Acid; D000001:Calcimycin; D010741:Phospholipases A