[Cloning and study of the expression of Pseudomonas aeruginosa cip1 gene by phage-transposon D3112 in a homologous host and in Escherichia coli].
Regulatory gene cipl of Pseudomonas aeruginosa transposable phage D3112 was cloned, and its expression was studied in P. aeruginosa and Escherichia coli. Overexpression of the cipl gene prevents transcription and replication of phage D3112 DNA and also lysogenization of bacteria P. aeruginosa PAO1 by phage D3112. The direction of cipl gene transcription within the vector was determined in the study of cipl gene expression, dependent on its orientation toward the gene lacZ promoter. The expression of the cloned cipl gene inhibited the specific TCS phenotype of E. coli (RP4 :: D3112) cells. The functional homology of the cipl gene of phage D3112 and the negative regulator ner of E. coli Mul phage was discussed.
Bidnenko
E M
EM
Akhverdian
V Z
VZ
Krylov
V N
VN
D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D004251:DNA Transposable Elements
Klonirovanie i izuchenie ékspressii gena cip1 faga-transpozona D3112 Pseudomonas aeruginosa v gomologichnom khoziaine i v Escherichia coli.