title: Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay.
abstract: A rapid multiplex PCR assay was developed to distinguish between North American and European genotypes of porcine reproductive and respiratory syndrome (PRRS) virus after a portion of the polymerase gene (open reading frame 1b) was sequenced for two North American PRRS virus strains. DNA products with unique sizes characteristic of each genotype were obtained.
authors.affiliation: Animal Diseases Research Institute, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Gilbert
authors.forename: R
authors.initials: R
authors.lastname: Larochelle
authors.forename: R
authors.initials: R
authors.lastname: Magar
authors.forename: H J
authors.initials: HJ
authors.lastname: Cho
authors.forename: D
authors.initials: D
authors.lastname: Deregt
mesh_terms: D000818:Animals; D001483:Base Sequence; D005060:Europe; D005838:Genotype; D008969:Molecular Sequence Data; D009656:North America; D016133:Polymerase Chain Reaction; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine / Q000821:virology*