title: The VP3 gene of human group C rotavirus.
abstract: The complete nucleotide sequence of genome segment 4 from the human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequences of the genome termini with the consensus 5' and 3' terminal non-coding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequence motifs. Human group C rotavirus genome segment 4 is 2,166bp long and encodes a single open reading frame of 2,082 nucleotides (693 amino acids) starting at nucleotide 55 and terminating at nucleotide 2,136 giving a 3' untranslated region of 30 nucleotides. Alignment with the porcine group C VP3 equivalent gene showed the human gene is one amino acid longer, and that the proteins have 84.1% amino acid sequence identity. A conserved potential nucleotide binding motif shared with the porcine VP3 sequence was identified. Analogy with the group A rotaviruses suggested that the genome segment 4 encodes the group C rotavirus guanylyltransferase.
authors.affiliation: University Medical School, Southampton General Hospital, United Kingdom.
authors.forename: A R
authors.initials: AR
authors.lastname: Samarbaf-Zadeh
authors.forename: P R
authors.initials: PR
authors.lastname: Lambden
authors.forename: S M
authors.initials: SM
authors.lastname: Green
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.forename: E O
authors.initials: EO
authors.lastname: Caul
authors.forename: I N
authors.initials: IN
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C442546:VP3 protein, Rotavirus