title: Electrospray ionization Fourier transform ion cyclotron resonance at 9.4 T.
abstract: We present the first results from a new electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer operated at a magnetic field of 9.4 T (i.e. > or = 2.4 T higher than for any prior FTICR instrument). The 9.4 T instrument provides substantially improved performance for large molecules (> or = 50% increase in mass resolving power) and complex mixtures (> or = 100% increase in dynamic range) compared to lower-field (< or = 6 T) instruments. The higher magnetic field makes possible larger trapped-ion population without introduction of significant space--charge effects such as spectral peak shift and/or distortion, and coalescence of closely-spaced resonances. For bovine ubiquitin (8.6 kDa) we observe accurate relative isotopic abundances at a signal-to-noise ratio greater than 1000:1, whereas a complete nozzle-skimmer dissociation electrospray ionization (ESI) FTICR mass spectrum of bovine carbonic anhydrase (29 kDa) is achieved from a single scan with a signal-to-noise ratio of more than 250:1. Finally, we are able to obtain mass resolving power, m/delta m > 200,000, routinely for porcine serum albumin (67 kDa). The present performance guides further modifications of the instrument, which should lead to significant further improvements.
authors.affiliation: Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Florida State University, Tallahassee 32310, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Senko
authors.forename: C L
authors.initials: CL
authors.lastname: Hendrickson
authors.forename: L
authors.initials: L
authors.lastname: Pasa-Tolić
authors.forename: J A
authors.initials: JA
authors.lastname: Marto
authors.forename: F M
authors.initials: FM
authors.lastname: White
authors.forename: S
authors.initials: S
authors.lastname: Guan
authors.forename: A G
authors.initials: AG
authors.lastname: Marshall
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D017357:Cyclotrons; D004574:Electromagnetic Fields; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000295:instrumentation*; D012709:Serum Albumin / Q000737:chemistry; D014452:Ubiquitins / Q000737:chemistry
chemical_list: D012709:Serum Albumin; D014452:Ubiquitins; D002256:Carbonic Anhydrases