title: Co-culture of hypothalamic neurons and melanotrope cells: a model to study synaptogenesis between central neurons and endocrine cells.
abstract: As a first step towards elucidating mechanisms involved in neuroendocrine synaptogenesis, we developed a model of co-culture based on hypothalamic-intermediate pituitary interactions. Dissociated hypothalamic neurons from fetal rats at embryonic day 15 were cultured in a defined medium together with melanotrope cells of the pituitary intermediate lobe from neonatal rats. In these co-cultures, establishment of synaptic contacts between GABAergic or dopaminergic neurons and an endocrine target cell the melanotrope cell, was studied by morphofunctional approaches. Using double immunostaining with antibodies directed against glutamate decarboxylase or tyrosine hydroxylase and alpha-melanocyte-stimulating hormone, we demonstrated morphological contacts between GABAergic or dopaminergic neurons and melanotrope cells as early as three days in vitro. Furthermore, using an antibody directed against synapsin I, we showed a modification of synapsin I immunoreactivity from diffuse to punctate distribution correlated with the establishment of contacts and the observation of characteristic neuroendocrine synapses by electron microscopy. These results were further confirmed by electrophysiological studies. Patch-clamp recordings demonstrated that, at six days in vitro, some melanotrope cells displayed GABAergic synaptic currents, which occurred either spontaneously and/or could be evoked chemically by 50 mM KCl or 100 microM kainate. The proportion of the melanotrope cells receiving functional synaptic inputs increased until 10 days in culture, a stage at which virtually all melanotrope cells in contact with neurons possessed functional synapses. The results presented here describe the establishment of neuroendocrine synapses in vitro, studied by combining morphofunctional and electrophysiological approaches.
authors.affiliation: Laboratoire de Neurophysiologie et de Neurobiologie des Systèmes Endocrines, Université Louis Pasteur, URA CNRS 1446, Strasbourg, France.
authors.forename: F
authors.initials: F
authors.lastname: René
authors.forename: P
authors.initials: P
authors.lastname: Poisbeau
authors.forename: C
authors.initials: C
authors.lastname: Egles
authors.forename: R
authors.initials: R
authors.lastname: Schlichter
authors.forename: J M
authors.initials: JM
authors.lastname: Félix
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000502:physiology; D018920:Coculture Techniques; D004594:Electrophysiology; D007031:Hypothalamus / Q000166:cytology*; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D005680:gamma-Aminobutyric Acid; D009074:Melanocyte-Stimulating Hormones