The effect on maximal electroshock seizures induced by GABA agents and antiepileptic drugs microinfused into the nucleus reticularis pontis oralis.
The nucleus reticularis pontis oralis (RPO) is necessary for the expression of tonic hindlimb extension (THE) in maximal electroshock seizures (MES) of rats. Previous work in this laboratory has demonstrated that focal RPO microinfusion of NMDA antagonists inhibited THE while focal RPO microinfusion of NMDA induced convulsive activity similar to the audiogenic seizure response of rats. The purpose of the present study was to identify other receptors in the RPO that influence THE or induce convulsive activity. Bilateral microinfusion of bicuculline had no effect on the THE component of MES except when the bicuculline induced wild-running convulsions in which case the subsequent THE response to the MES stimulus was inhibited. The GABAergic agents muscimol, baclofen or 2-hydroxysaclofen neither altered the THE response nor induced convulsions. In addition, bilateral RPO microinfusion of the clinically effective antiepileptic drugs phenytoin, phenobarbital, valproate, ethosuximide or felbamate had no effect on the THE component of MES. These results indicate that the role of GABAergic receptors in the anticonvulsant activity mediated by the RPO is not prominent and that the RPO is unlikely to be the site of antiepileptic drug action in MES.
Peterson
S L
SL
College of Pharmacy, University of New Mexico Health Science Center, Albuquerque 87131-1066, USA.
D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D016009:Chi-Square Distribution; D004353:Drug Evaluation, Preclinical; D004597:Electroshock; D018682:GABA Agents / Q000627:therapeutic use*; D058785:GABA-A Receptor Agonists; D006614:Hindlimb; D007260:Infusion Pumps; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018080:Receptors, GABA-B / Q000187:drug effects; D012154:Reticular Formation / Q000187:drug effects*; D012640:Seizures / Q000188:drug therapy* / Q000209:etiology
D000927:Anticonvulsants; D018682:GABA Agents; D058785:GABA-A Receptor Agonists; D018080:Receptors, GABA-B