Stimulation of phospholipid synthesis in HeLa cells by epidermal growth factor and insulin: activation of choline kinase and glycerophosphate acyltransferase.
The cultivation of HeLa cells in the absence of growth factors reduced proliferation with a considerable decrease in the phospholipid content. A single addition of EGF or insulin was not enough to stimulate proliferation, but still sufficient to efficiently restore the phospholipid content to the level of serum-grown cells. Both EGF and insulin stimulated the synthesis of major phospholipids in HeLa cells. Irrespective of whether cells were stimulated or not, the phospholipid composition and the phospholipid/protein ratio remained constant, suggesting the existence of a mechanism that keeps them constant under varying conditions. The degradation of phosphatidylcholine was not affected by EGF or insulin. Both EGF and insulin increased choline kinase activity equally and promoted the conversion of choline to cholinephosphate, accompanied by an expansion of the cholinephosphate pool in treated cells. The level of glycerophosphate acyltransferase was enhanced by EGF and insulin but EGF was more effective than insulin. The present results provide evidence that one of the roles of EGF and insulin during cell growth is to stimulate the synthesis of phospholipids.
Uchida
T
T
Department of Biochemistry, Gunma University School of Medicine, Japan. tuchida@sb.gunma-u.ac.jp
D002794:Choline / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*; D010767:Phosphorylcholine / Q000378:metabolism
D004983:Ethanolamines; D007328:Insulin; D010710:Phosphates; D010713:Phosphatidylcholines; D010743:Phospholipids; D010767:Phosphorylcholine; D019856:Ethanolamine; D004815:Epidermal Growth Factor; D005992:Glycerol-3-Phosphate O-Acyltransferase; D002797:Choline Kinase; D002794:Choline; D005990:Glycerol