Rapid, high performance liquid chromatography-based, periodate-coupled thiobarbituric acid method for the determination of sialic acid in bronchoalveolar lavage fluid.
A simple, rapid high performance liquid chromatographic adaptation of the periodate-coupled thiobarbituric acid (TBA) method was developed for the quantification of sialic acid (SIAC) in supernatants from bronchoalveolar lavage fluids (BALF). Malondialdehyde released from BALF samples in various amounts did not interfere with the accurate quantification of SIAC.
Freyberger
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Bayer AG, Fachbereich Toxikologie, Wuppertal, Germany.
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D010504:Periodic Acid / Q000737:chemistry*; D013858:Thiobarbiturates / Q000737:chemistry*
D013858:Thiobarbiturates; D010504:Periodic Acid; D019158:N-Acetylneuraminic Acid; C029684:thiobarbituric acid