title: Identification and mapping of phosphocholine in animal tissue by static secondary ion mass spectrometry and tandem mass spectrometry.
abstract: Secondary ion mass spectra and images were obtained from animal tissue samples using less than 10(13) primary ions/cm2. The mass spectra showed abundant peaks at m/z 184 and m/z 86. Tandem mass spectrometry (MS/MS) was used to identify the source of these ions as phosphocholine. Secondary ion images obtained using MS/MS were used to show that m/z 86 is an abundant gas-phase fragment ion derived from m/z 184. These results are discussed in terms of the physiology of the samples investigated.
authors.affiliation: Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, TN 37831-6365, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: McMahon
authors.forename: R T
authors.initials: RT
authors.lastname: Short
authors.forename: C A
authors.initials: CA
authors.lastname: McCandlish
authors.forename: J T
authors.initials: JT
authors.lastname: Brenna
authors.forename: P J
authors.initials: PJ
authors.lastname: Todd
mesh_terms: D000311:Adrenal Glands / Q000033:anatomy & histology / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D004285:Dogs; D007091:Image Processing, Computer-Assisted; D009316:National Institutes of Health (U.S.); D010713:Phosphatidylcholines / Q000032:analysis*; D018629:Spectrometry, Mass, Secondary Ion; D013552:Swine; D014481:United States
chemical_list: D010713:Phosphatidylcholines