Local actions of acetylcholine on vasomotor regulation in rat incisor pulp.
Local actions of acetylcholine on vasomotor regulation in rat incisor pulp were investigated in anaesthetized animals. Pulpal blood flow was measured with laser Doppler flowmetry. lontophoresis (40 or 60 muA for 20 s) with acetylcholine or carbachol on an exposed dentin surface caused a biphasic response: an initial vasoconstriction was followed by an atropinesensitive vasodilation. The vasoconstrictor response was enhanced by 171% in the presence of atropine, whereas in control experiments, using isotonic saline as a medium for the direct current, similar vasoconstriction was unaffected by atropine. Carbachol-induced vasodilation was significantly reduced by 30% after intravenous injection of the nitric oxide synthesis blocker N omega-nitro-L-arginine methyl ester (10 mg kg-1), and the remaining vasodilation was abolished by atropine. The present results support the concept of a cholinergic modulation of sympathetic vasoconstrictor function and of a partial contribution of nitric oxide in carbachol-induced vasodilation in rat incisor pulp.
Olgart
L
L
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Kostouros
G D
GD
Edwall
L
L
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D003782:Dental Pulp / Q000098:blood supply / Q000187:drug effects* / Q000502:physiology; D007180:Incisor / Q000098:blood supply / Q000187:drug effects* / Q000502:physiology; D007478:Iontophoresis; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects
D009569:Nitric Oxide; D001285:Atropine; D002217:Carbachol; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester