New class of poly(vinyl alcohol) polymers as column-chromatography stationary phases for Candida rugosa lipase isoforms separation.
The preparation of new stationary phases of cross-linked polyvinyl alcohol esterified with various linear fatty acids is described. The physico-chemical properties of these polymers are reported, including electron microscopy and swelling measurements. Batch adsorption experiments were performed in order to characterize the basic separative properties of these phases. Cross-linked polyvinyl alcohol esterified with dodecanoic acid was used for hydrophobic interaction chromatography of a commercial crude preparation of Candida rugosa lipase. Characterization of the purified fractions was carried out via native electrophoresis and sodium dodecyl sulfate-polyacrylamide electrophoresis.
Battinelli
L
L
Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Italy.
Cernia
E
E
Delbò
M
M
Ortaggi
G
G
Pala
A
A
Soro
S
S
D002175:Candida / Q000201:enzymology*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D011142:Polyvinyl Alcohol / Q000737:chemistry*
D011108:Polymers; D011142:Polyvinyl Alcohol; D008049:Lipase