title: Nerve regeneration and origin of Schwann cells in peripheral nerve allografts in immunologically pretreated rats.
abstract: For peripheral nerve allografts, the conditions for successful nerve regeneration and the possibility of transplanting Schwann cells were examined using a model of pretreated rats. Incomplete immunosuppression was achieved in recipient rats with donor red blood cell infusions given before allogeneic nerve grafting (RBC group). The origin of Schwann cells in the grafts was assessed by immunohistochemical staining from 1 week to 12 weeks after transplantation. In the RBC group, the replacement of donor Schwann cells by recipient cells was detected at 3-8 weeks, with the graft being filled with many of these cells at all times, and successful nerve regeneration was seen after 12 weeks on morphometric and electrophysiologic evaluations. In peripheral nerve allografts, pretreatment with donor-specific blood transfusion did not induce significant immunosuppression compared with allotransplantations of some tissues and organs. Clinically, if the state of immunosuppression can be controlled by RBC transfusion, it is possible that donor tissues may be replaced by recipient tissues and that nerves will regenerate successfully.
authors.affiliation: Department of Orthopaedic Surgery, Yamaguchi University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Katsube
authors.forename: K
authors.initials: K
authors.lastname: Doi
authors.forename: T
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Fujikura
authors.forename: M
authors.initials: M
authors.lastname: Shigetomi
authors.forename: S
authors.initials: S
authors.lastname: Kawai
mesh_terms: D000818:Animals; D001782:Blood Donors; D001803:Blood Transfusion; D004912:Erythrocytes / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D007518:Isoantibodies / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000637:transplantation*; D051381:Rats; D012583:Schwann Cells / Q000276:immunology*; D019172:Transplantation Conditioning
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies