title: Silica exposure increases expression of pulmonary intercellular adhesion molecule-1 (ICAM-1) in C57Bl/6 mice.
abstract: Although the pathogenic mechanisms underlying silica-induced lung damage are well described, few studies have examined the expression and role of adhesion molecules in lung injury induced by this particle. Here we report that intratracheal instillation of silica crystals (alpha quartz) (SI) into the lungs of C57Bl/6 mice results in a significant increase in levels of intercellular adhesion molecule-1 (ICAM-1) in lung tissue and in lung lavage fluid. This increased expression of ICAM-1 appeared to be associated with later (> or = 24 h) cell influx and lung injury rather than in the initiation of these events. Exposure of mice to the nontoxic particle titanium dioxide did not elicit increased expression of ICAM-1 in lung tissue or lavage fluid. Passive administration of rat anti-mouse ICAM-1 monoclonal antibody significantly decreased the influx of neutrophils (PMNs) into the alveoli and the levels of lung tissue ICAM-1 and yet had no effect on measures of lung injury or increased collagen deposition. These data suggest that increased ICAM-1 expression in lung tissue following exposure to silica plays a partial role in the trafficking of neutrophils into the airways.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs 06269, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Nario
authors.forename: A K
authors.initials: AK
authors.lastname: Hubbard
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006909:Hydroxyproline / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism; D055370:Lung Injury; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D012822:Silicon Dioxide / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000911:Antibodies, Monoclonal; D014874:Water Pollutants, Chemical; D018799:Intercellular Adhesion Molecule-1; C009495:titanium dioxide; D012822:Silicon Dioxide; D003094:Collagen; D014025:Titanium; D006909:Hydroxyproline