title: Comparison of the NMR and X-ray structures of the HIV-1 matrix protein: evidence for conformational changes during viral assembly.
abstract: The three-dimensional solution- and solid-state structures of the human immunodeficiency virus type-1 (HIV-1) matrix protein have been determined recently in our laboratories by NMR and X-ray crystallographic methods (Massiah et al. 1994. J Mol Biol 244:198-223; Hill et al. 1996. Proc Natl Acad Sci USA 93:3099-3104). The matrix protein exists as a monomer in solution at low millimolar protein concentrations, but forms trimers in three different crystal lattices. Although the NMR and X-ray structures are similar, detailed comparisons have revealed an approximately 6 A displacement of a short 3(10) helix (Pro 66-Gly 71) located at the trimer interface. High quality electron density and nuclear Overhauser effect (NOE) data support the integrity of the X-ray and NMR models, respectively. Because matrix apparently associates with the viral membrane as a trimer, displacement of the 3(10) helix may reflect a physiologically relevant conformational change that occurs during virion assembly and disassembly. These findings further suggest that Pro 66 and Gly 71, which bracket the 3(10) helix, serve as "hinges" that allow the 3(10) helix to undergo this structural reorientation.
authors.affiliation: Howard Hughes Medical Institute, University of Maryland, Baltimore 21228, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Massiah
authors.forename: D
authors.initials: D
authors.lastname: Worthylake
authors.forename: A M
authors.initials: AM
authors.lastname: Christensen
authors.forename: W I
authors.initials: WI
authors.lastname: Sundquist
authors.forename: C P
authors.initials: CP
authors.lastname: Hill
authors.forename: M F
authors.initials: MF
authors.lastname: Summers
mesh_terms: D018360:Crystallography, X-Ray; D015683:Gene Products, gag / Q000737:chemistry* / Q000502:physiology; D015488:HIV Antigens / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D014764:Viral Proteins; D019065:Virus Assembly; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D015683:Gene Products, gag; D015488:HIV Antigens; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1