Syntheses of functionalized indium porphyrins for monoclonal antibody labeling.
The syntheses and characterizations of five differently hydrosoluble monosubstituted aryl porphyrins are reported. Their metallation with indium-111 was achieved and provided tracers with strong specific activities. The covalent coupling between indium-111 porphyrins and BSA served as a model reaction for the definition of the best experimental coupling conditions; the transposition to the labeling of anti-CEA monoclonal antibody was realized. The conjugates exhibited an in vitro good mAb-labeling efficiency, as well as a good preservation of immunoreactivity.
Bedel-Cloutour
C H
CH
Laboratoire de Chimie Organique et Organométallique, URA 35, Université Bordeaux I, Talence, France.
Mauclaire
L
L
Saux
A
A
Pereyre
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D007205:Indium Radioisotopes; D009682:Magnetic Resonance Spectroscopy; D011166:Porphyrins / Q000737:chemistry*; D012709:Serum Albumin
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D011166:Porphyrins; D012709:Serum Albumin