Assignment of the CD45-AP gene to the centromeric end of mouse chromosome 19 and human chromosome 11q13.1-q13.3.
CD45-AP is a recently identified phosphorylated protein that specifically associates with the leukocyte-specific transmembrane glycoprotein CD45. The gene for CD45-AP, Ptprcap (protein tyrosine phosphatase, receptor type c polypeptide associated protein), was mapped in mouse by typing the progeny of two multilocus crosses using the mouse CD45-AP cDNA as a Southern hybridization probe. The CD45-AP gene mapped to the centromeric region of Chr 19 proximal to the genes Fth, Cd5, and Pcna-rs. The gene for the human CD45-AP homologue, PTPRCAP, was localized to chromosome band 11q13.1-q13.3 by fluorescence in situ hybridization using human genomic CD45-AP DNA as a hybridization probe. The genetic mapping of the Ptprcap/PTPRCAP genes extends the previously defined synteny conservation of various genes that have been assigned to these regions of the mouse and the human chromosomes.
Takai
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Department of Genetics, International Medical Center of Japan, Tokyo, 162, Japan. Akiko_Takeda@brown.edu
Kozak
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CA
Kitamura
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K
Takeda
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A
D000818:Animals; D002503:Centromere / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051379:Mice / Q000235:genetics*; D009115:Muridae / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics*; D013045:Species Specificity
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C090519:PTPRCAP protein, human; D010750:Phosphoproteins; C513303:Ptprcap protein, mouse