title: Isolation of four novel neuropeptides, the hydra-RFamides I-IV, from Hydra magnipapillata.
abstract: Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated four novel peptides from extracts of Hydra magnipapillata: <Glu-Trp-Leu-Gly-Gly-Arg-Phe-NH2 (Hydra-RFamide I), <Glu-Trp-Phe-Asn-Gly-Arg-Phe-NH2 (Hydra-RFamide II), Lys-Pro-His-Leu-Arg-Gly-Arg-Phe-NH2 (Hydra-RFamide III) and His-Leu-Arg-Gly-Arg-Phe-NH2 (Hydra-RFamide IV). The Hydra-RFamides I-IV are neuropeptides. They form a peptide family with other known neuropeptides from hydromedusae, sea anemones and sea pansies.
authors.affiliation: Department of Cell Biology and Anatomy, University of Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Moosler
authors.forename: K L
authors.initials: KL
authors.lastname: Rinehart
authors.forename: C J
authors.initials: CJ
authors.lastname: Grimmelikhuijzen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D006829:Hydra / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification*; D011487:Protein Conformation
chemical_list: D009479:Neuropeptides