title: Hemoglobin adducts of benzo[a]pyrene diolepoxide in newspaper vendors: association with traffic exhaust.
abstract: Benzo[a]pyrene diol epoxide adducts with hemoglobin (Hb) were measured to detect human exposure to environmental benzo[a]pyrene from traffic exhaust. Benzo[a]pyrene tetrahydrotetrols (BPTs) released from Hb after acid hydrolysis were quantitated by gas chromatography-mass spectrometry after immunoaffinity chromatography. Fifty three newspaper vendors were enrolled. The median adduct concentration was 0.3 fmol BPTs/mg Hb in high density traffic-exposed vendors and < or = 0.1 fmol BPTs/mg Hb in those exposed to low density traffic; the difference was not significant (P = 0.09). Among non-smokers, adducts were detectable in 60% of high exposure subjects (median 0.3 fmol BPTs/mg Hb) and in 28% of those with low exposure (median < or = 0.1 fmol/mg Hb). This difference was significant (P = 0.02). In low exposure smokers the median of adducts was 0.26 fmol BPTs/mg Hb, while in low exposure non-smokers it was < or = 0.1 fmol BPTs/mg Hb (P = 0.08, not significant). Adduct concentration was no different for low and high density traffic-exposed smokers (P = 0.82). The data indicate a significant difference in adduct concentration related to traffic exhaust exposure among non-smokers.
authors.affiliation: Laboratory of Molecular Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Pastorelli
authors.forename: J
authors.initials: J
authors.lastname: Restano
authors.forename: M
authors.initials: M
authors.lastname: Guanci
authors.forename: M
authors.initials: M
authors.lastname: Maramonte
authors.forename: C
authors.initials: C
authors.lastname: Magagnotti
authors.forename: R
authors.initials: R
authors.lastname: Allevi
authors.forename: D
authors.initials: D
authors.lastname: Lauri
authors.forename: R
authors.initials: R
authors.lastname: Fanelli
authors.forename: L
authors.initials: L
authors.lastname: Airoldi
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis* / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008875:Middle Aged; D016273:Occupational Exposure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012907:Smoking / Q000009:adverse effects; D013056:Spectrophotometry, Ultraviolet; D001335:Vehicle Emissions
chemical_list: D006454:Hemoglobins; D001335:Vehicle Emissions; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide