Primary sequences of rat mu-calpain large and small subunits are, respectively, moderately and highly similar to those of human.
cDNAs for rat mu-calpain large subunit and the calpain small subunit were cloned and sequenced. The large subunit encodes 713 amino-acid residues, which includes one deletion compared to that of human. The overall similarity is 89% to human mu-type, which is slightly lower than those compared between other types of calpain large subunits of rat and human (93-94%). On the other hand, the small subunit showed high conservation, being 94.0% identical to that of human. With these sequences, primary structures of all rat calpain subunits that have been considered to exist were completely elucidated.
Sorimachi
H
H
Department of Molecular Biology, University of Tokyo, Japan. i34491@m-unix.cc.u-tokyo.ac.jp
Amano
S
S
Ishiura
S
S
Suzuki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002154:Calpain / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013270:Stomach
D018076:DNA, Complementary; D002154:Calpain