title: Specific antigens of Chlamydia pecorum and their homologues in C psittaci and C trachomatis.
abstract: OBJECTIVE
  To analyze the antigenic cross reactivity of various proteins of strains of Chlamydia pecorum, C psitaci, and C trachomatis. SAMPLES AND PROCEDURES: Strains FC-Stra and LW-613 of C pecorum, strains B577, Fitz-9, and 68C of C psitaci, and strain LGV-2 of C trachomatis were studied. Strains of C pecorum were propagated in Georgia bovine kidney cells, and other chlamydial strains were propagated in L cells or Georgia bovine kidney cells. Partially purified chlamydial elementary bodies propagated in RAG cells, a BALB/c cell line cloned from a renal adenocarcinoma of BALB/c mice, were used to immunize BALB/c mice for production of monoclonal antibodies. Rabbits were inoculated with yolk sack-propagated, purified elementary bodies to produced polyclonal antisera. The reaction of monoclonal antibodies and polyclonal antisera with chlamydial proteins was analyzed by use of immunoblot techniques.
  
  
  RESULTS
  Two monoclonal antibodies reacted with a 90-kd protein of C psittaci and a 94-kd protein of C pecorum strains. One monoclonal antibody reacted strongly with a 67-kd protein of C pecorum and strain B577 of C psittaci, but weakly with proteins of strains 6BC and LGV-2. Another monoclonal antibody reacted with a 46-kd protein of 2 C pecorum strains and of strain B577 of psittaci, but not with those of strains 6BC and LGV-2. Two monoclonal antibodies reacted with a 20-kd protein of C pecorum and a 22-kd protein of C psittaci and LGV-2 strains. Polyclonal antisera reacted similarly with the proteins identified by monoclonal antibodies in the various chlamydial strains.
  
  
  CONCLUSIONS
  Reactions of several monoclonal antibodies with chlamydial antigens indicated that 67- and 46-kd proteins contain genus- and species-specific epitopes, respectively; a 94-kd protein of C pecorum is homologous to a 90-kd protein of C psittaci and C trachomatis strains; and a 20-kd protein of C pecorum corresponds to a 22-kd protein of C psittaci and C trachomatis.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine Louisiana State University, Baton Rouge 70803-8416, USA.
authors.forename: A
authors.initials: A
authors.lastname: Baghian
authors.forename: K
authors.initials: K
authors.lastname: Kousoulas
authors.forename: R
authors.initials: R
authors.lastname: Truax
authors.forename: J
authors.initials: J
authors.lastname: Storz
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D002689:Chlamydia / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003429:Cross Reactions; D015151:Immunoblotting; D007668:Kidney; D007680:Kidney Neoplasms; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins