Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8).
The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or HHV8) was mapped with cosmid and phage genomic libraries from the BC-1 cell line. Its nucleotide sequence was determined except for a 3-kb region at the right end of the genome that was refractory to cloning. The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region flanked by multiple G + C-rich 801-bp terminal repeat sequences. A genomic duplication that apparently arose in the parental tumor is present in this cell culture-derived strain. At least 81 ORFs, including 66 with homology to herpesvirus saimiri ORFs, and 5 internal repeat regions are present in the long unique region. The virus encodes homologs to complement-binding proteins, three cytokines (two macrophage inflammatory proteins and interleukin 6), dihydrofolate reductase, bcl-2, interferon regulatory factors, interleukin 8 receptor, neural cell adhesion molecule-like adhesin, and a D-type cyclin, as well as viral structural and metabolic proteins. Terminal repeat analysis of virus DNA from a KS lesion suggests a monoclonal expansion of KSHV in the KS tumor.
Russo
J J
JJ
Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Bohenzky
R A
RA
Chien
M C
MC
Chen
J
J
Yan
M
M
Maddalena
D
D
Parry
J P
JP
Peruzzi
D
D
Edelman
I S
IS
Chang
Y
Y
Moore
P S
PS
D001483:Base Sequence; D002874:Chromosome Mapping; D005814:Genes, Viral; D016679:Genome, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012514:Sarcoma, Kaposi / Q000821:virology*