SLE autoantibodies recognize spermine induced Z-conformation of native calf thymus DNA.
The binding specificity of naturally occurring anti-DNA antibodies with spermine modified DNA was analyzed by direct binding and competition ELISA. The binding was visualised by band shift assay. The SLE anti-DNA antibodies appreciably recognized the spermine induced Z-conformation of native DNA. The degree of binding of these antibodies to the Z-conformer was almost similar to their binding with native DNA. The spermine-DNA complex was found to be thermodynamically more stable than native DNA.
Ali
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Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Ali
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D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009690:Nucleic Acid Conformation; D011489:Protein Denaturation; D013056:Spectrophotometry, Ultraviolet; D013096:Spermine / Q000378:metabolism*; D013816:Thermodynamics; D013950:Thymus Gland / Q000737:chemistry
D000974:Antibodies, Antinuclear; D013096:Spermine; D004247:DNA