Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters.
We have cloned and sequenced mouse utrophin cDNA, and successfully expressed full length utrophin (400 kDa) in both muscle and non-muscle cells. The expression of recombinant utrophin is compared with that of its homologue, dystrophin (427 kDa). We demonstrate that recombinant utrophin is targeted into agrin-induced acetylcholine receptor (AChR) clusters, while recombinant dystrophin is evenly distributed along cell membranes in cultured Sol 8 muscle cells. This observation suggests that utrophin and dystrophin may interact with different cytoskeletal proteins. The C-terminal domains are found to be responsible for the association of utrophin with AChR clusters.
Guo
W X
WX
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA. aguo@pharmdec.wustl.edu
Nichol
M
M
Merlie
J P
JP
D018171:Agrin / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016189:Dystrophin / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D014162:Transfection; D049411:Utrophin
D018171:Agrin; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008565:Membrane Proteins; D011950:Receptors, Cholinergic; D011994:Recombinant Proteins; C483600:Utrn protein, mouse; D049411:Utrophin