Electrogenicity at the donor/acceptor sides of cyanobacterial photosystem I.
To study electrogenesis the photosystem I particles from Synechococcus elongatus were incorporated into asolectin liposomes, and fast kinetics of laser flash-induced electric potential difference generation has been measured by a direct electrometric method in proteoliposomes absorbed on a phospholipid-impregnated collodion film. The photoelectric response has been found to involve three electrogenic stages associated with (i) iron-sulfur center Fx reduction by the primary electron donor P700, (ii) electron transfer between iron-sulfur centers Fx and FA/FB, and (iii) reduction of photo-oxidized P700+ by reduced cytochrome C553. The relative magnitudes of phases (ii) and (iii) comprised about 20% of phase (i).
Mamedov
M D
MD
A. N. Belozersky Institute of Physico-chemical Biology, Moscow State University, Russia.
Gadzhieva
R M
RM
Gourovskaya
K N
KN
Drachev
L A
LA
Semenov Ayu
D018360:Crystallography, X-Ray; D000458:Cyanobacteria; D007700:Kinetics; D008081:Liposomes; D008564:Membrane Potentials; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045331:Photosystem I Protein Complex; D011487:Protein Conformation
D008081:Liposomes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex