Osteoinductive potential of freeze-dried, biodegradable, poly (glycolic acid-co-lactic acid) disks incorporated with bone morphogenetic protein in skull defects of rats.
Biodegradable, porous, polymer implant disks with an osteoinductive potential were prepared by a freeze-drying technique by incorporating bovine bone morphogenetic protein (BMP) in poly (glycolic acid-co-lactic acid) (PGLA). The PGLA disks with and without BMP were implanted in rat skull defects, and the defect sites were studied radiographically and histologically for 2 and 4 weeks after implantation. A quantitative radiographic analysis showed significantly thicker radiopacity for the disks with BMP than those without BMP (P < 0.01). After 2 weeks, chondrogenesis and new bone formation were observed on the BMP-incorporated disks. After 4 weeks, PGLA was completely replaced by new bone in the defects with implantation of the BMP-incorporated disks, whereas the defects implanted with PGLA alone were filled with fibrous connective tissue. The results suggest that BMP-incorporated PGLA is an ideal bone substitute with osteoinductive potential.
Miki
T
T
Second Department of Oral Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Imai
Y
Y
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D001853:Bone Marrow / Q000000981:diagnostic imaging / Q000473:pathology; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002356:Cartilage / Q000000981:diagnostic imaging / Q000473:pathology; D002417:Cattle; D003238:Connective Tissue / Q000000981:diagnostic imaging / Q000473:pathology; D005500:Follow-Up Studies; D005612:Freeze Drying; D019344:Lactic Acid; D008567:Membranes, Artificial; D016348:Osseointegration; D010012:Osteogenesis; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D016062:Porosity; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)