Nucleic acid-8-methoxypsoralen crosslinks bind monoclonal anti-Z-DNA antibody.
Native calf thymus DNA and poly(dA-dT).poly(dA-dT) were photo-adducted with 8-methoxypsoralen and characterized by thermal denaturation (Tm) and hydroxyapatite column chromatography. The data demonstrated the formation of interstrand photo-crosslinks. It has been shown by competition ELISA and band shift assays that crosslinked species of DNA-8-MOP and poly(dA-dT)-8-MOP photoadducts recognize previously defined monoclonal anti-Z-DNA antibody (Z22). The results indicate the possible presence of Z- or Z-like epitopes on nucleic acid-8-MOP crosslinks as Z22 antibody does not recognize other nucleic acid conformations. These studies also point out that conformational changes in DNA arising from the photo-addition could induce antibodies to DNA or could cause autoimmune disease.
Arif
Z
Z
Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A. M. U. Aligarh, India.
Ali
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R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D018736:DNA Adducts / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D008730:Methoxsalen / Q000737:chemistry / Q000276:immunology*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011067:Poly dA-dT / Q000737:chemistry / Q000276:immunology*; D013696:Temperature
C057863:8-methoxypsoralen-DNA; D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D018736:DNA Adducts; D000939:Epitopes; D011067:Poly dA-dT; D004247:DNA; D008730:Methoxsalen