title: Force production by chemically crosslinked myosin-actin crossbridges in rabbit skinned fibers in response to MgATP depletion.
abstract: In order to study the contractile property of myosin crossbridges attached to thin filaments, myosin heads were crosslinked to the filaments at their interface in single skinned rabbit psoas fibers with a zero-length chemical crosslinker, 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (EDC). The results obtained show that a partially crosslinked single fiber produces a large rigor-like force when MgATP is depleted from the myofibrillar space. Such crosslinked fibers contain two types of crosslinked myosin heads: one with one of the two heads of the myosin molecule crosslinked to actin with the other head uncrosslinked; the other has both heads crosslinked to actin. The results of this work suggest that a crosslinked myosin head of the former type produces a much larger force than the latter type.
authors.affiliation: Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Emoto
authors.forename: K
authors.initials: K
authors.lastname: Tawada
mesh_terms: D000199:Actins / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology*; D009218:Myosins / Q000737:chemistry*; D011817:Rabbits
chemical_list: D000199:Actins; D003432:Cross-Linking Reagents; D000255:Adenosine Triphosphate; D009218:Myosins; D005022:Ethyldimethylaminopropyl Carbodiimide