title: Telomerase activity in reactive and neoplastic lymphoid tissues: infrequent detection of activity in Hodgkin's disease.
abstract: We used the recently described sensitive and rapid detection assay called telomeric repeat amplification protocol (TRAP) to detect telomerase activity in lymphoblastoid (n = 5) and lymphoma cell lines (n = 7), hyperplastic lymph nodes (n = 6) and tonsils (n = 5), and tissues involved by non-Hodgkin's lymphoma (NHL) (n = 43) and Hodgkin's disease (HD) (n = 14). Clearly evident telomerase activity was found in all lymphoblastoid and lymphoma cell lines, and in 34 of 43 cases (80%) of NHL. These results were expected because of the proliferative and immortal nature of the cell lines and most malignant cells. However, positive results were obtained with the TRAP assay in all hyperplastic lymph nodes and tonsils, which raises the issue of derepression of telomerase activity during an immune response. Telomerase activity alone therefore does not distinguish malignant lymphoid proliferations from reactive states. Telomerase activity was detected in only 1 of 14 cases (7%) of lymphoid tissues involved by HD. Eight of the 13 negative cases were considered to be interpretable because of the lack (3 of 13 cases) or low level (5 of 13 cases) of telomerase inhibition. The five remaining cases could not be evaluated because of their telomerase inhibitor content. The findings imply either transient or very low levels of telomerase activity in HD or that HD for the greater part is a telomerase-independent neoplasm. Microdissection studies are needed to identify subsets of cells carrying telomerase activity in both reactive and neoplastic lymphoid tissues.
authors.affiliation: Laboratoire du Groupe d'Etude des Lymphomes Malins (CNRS, CIGH), Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Brousset
authors.forename: T
authors.initials: T
authors.lastname: al Saati
authors.forename: N
authors.initials: N
authors.lastname: Chaouche
authors.forename: R C
authors.initials: RC
authors.lastname: Zenou
authors.forename: D
authors.initials: D
authors.lastname: Schlaifer
authors.forename: S
authors.initials: S
authors.lastname: Chittal
authors.forename: G
authors.initials: G
authors.lastname: Delsol
mesh_terms: D002877:Chromosomes, Human / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006965:Hyperplasia; D008198:Lymph Nodes / Q000473:pathology; D008221:Lymphoid Tissue / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism* / Q000473:pathology; D014066:Palatine Tonsil / Q000473:pathology; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D019098:Telomerase