title: [Repeated administration of protamine attenuates protamine-induced systemic hypotension].
abstract: Protamine may act on endothelial cell receptors to stimulate the production of nitric oxide, which would promote vasodilation. If so, the repeated administration of protamine may attenuate protamine-induced systemic hypotension. To confirm this hypothesis, we examined whether repeated administration of protamine attenuates protamine-induced systemic hypotension in rats. Rats were divided into two groups. In bolus injection group (S group), protamine was given intravenously at 10 mg.kg-1. In repeated administered group (R group), the same dose of protamine was given intravenously 30 min after continuous infusion of protamine 10 mg.kg-1. The mean arterial pressure in S group decreased significantly after administration of protamine, compared with R group. Also, maximal decrease rate of the arterial pressure after administration of protamine in R group was significantly lower than in S group. These results demonstrate that repeated administration of protamine attenuates protamine-induced systemic hypotension. We confirm the hypothesis that protamine will release nitric oxide by binding with the receptor on the endothelium in the vessel and subsequently cause systemic hypotension.
authors.affiliation: Department of Anesthesiology, Hiraka General Hospital, Yokote.
authors.forename: H
authors.initials: H
authors.lastname: Komatsu
authors.forename: K
authors.initials: K
authors.lastname: Enzan
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004727:Endothelium / Q000378:metabolism; D007022:Hypotension / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D011479:Protamines / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011479:Protamines; D009569:Nitric Oxide