Ubiquitous expression and testis-specific alternative polyadenylation of mRNA for the human Ran GTPase activator RanGAP1.
RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleo-cytoplasmic transport of both, proteins and mRNAs, and also in cell cycle regulation. Here, we report a 2970-bp cDNA clone of RanGAP1 isolated from a HeLa lambda gt11 cDNA library. It contains a 215-bp 5' untranslated region (UTR) with a G + C-content of 68%, followed by a 1764-bp open reading frame and a 989-bp 3' UTR preceding a 77-bp poly(A)+ tail. RanGAP1 shows differential patterns of expression in human tissues. In addition to the 3.5-kb transcript present in all tissues and highly expressed in brain, thymus and testis, we found a second transcript of 2.8 kb in testis. In order to analyze this shorter transcript, we screened a human testis lambda gt10 cDNA library and cloned an alternatively polyadenylated RanGAP1 transcript. Taking the 3' UTR of RanGAP1, which lies downstream of the first polyadenylation signal, as a probe in Northern blot analysis, we confirmed that this second transcript found in testis results from a distinct 3' UTR.
Krebber
H
H
Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg, Germany.
Ponstingl
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D018076:DNA, Complementary; D004789:Enzyme Activation; D005091:Exons; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D020931:ran GTP-Binding Protein
D002352:Carrier Proteins; D015342:DNA Probes; D018076:DNA, Complementary; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; C092317:RANGAP1 protein, human; D012333:RNA, Messenger; D011061:Poly A; D020558:GTP Phosphohydrolases; D020931:ran GTP-Binding Protein