title: Novel agglutinin in the midgut of the tick Ixodes ricinus.
abstract: Haemagglutination activity (HA) was found and characterized in a midgut homogenate of Ixodes ricinus (L.). HA was induced by tick feeding; it was not detected in starved ticks. In a haemagglutination inhibition test, HA showed an affinity for some carbohydrates (N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, rhamnose, and dulcit) and glycoconjugates (especially lipopolysaccharides). Midgut protein components of 37, 60, 65, and 73 kDa were identified by immunoblotting as potential structural subunits of the new agglutinin.
authors.affiliation: Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Uhlír
authors.forename: L
authors.initials: L
authors.lastname: Grubhoffer
authors.forename: P
authors.initials: P
authors.lastname: Volf
mesh_terms: D000818:Animals; D002241:Carbohydrates; D004064:Digestive System / Q000276:immunology; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000032:analysis* / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D018884:Ixodes / Q000276:immunology*; D008970:Molecular Weight; D011134:Polysaccharides
chemical_list: D002241:Carbohydrates; D006388:Hemagglutinins; D011134:Polysaccharides