Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli.
A synthetic gene encoding the aminoacid sequence of the sweet-tasting protein thaumatin II has been assembled and expressed in Escherichia coli. Immunoblotting analysis shows that the expressed recombinant thaumatin has the same molecular weight as the protein from its natural source, the plant Thaumatoccocus daniellii Benth.
Faus
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I
Laboratory of Biotechnology, URQUIMA, S.A. (URIACH GROUP), Sant Fost de Campsenteiles (Barcelona), Spain.
Patiño
C
C
Río
J L
JL
del Moral
C
C
Barroso
H S
HS
Rubio
V
V
D000918:Antibody Specificity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D005822:Genetic Vectors; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis; D013549:Sweetening Agents / Q000378:metabolism*
D010940:Plant Proteins; D011994:Recombinant Proteins; D013549:Sweetening Agents; C057542:thaumatin II protein, Thaumatococcus daniellii