[Evaluation of methods of sperm preparation for human in vitro fertilization].
OBJECTIVE
Compare results of sperm prepared with centrifiguation-migration, percoll discontinuous gradient (50, 70, 90%) and migration-sedimentation.


METHODS
An aliquot of sperm collected from 125 men referred for infertility was prepared according to the three methods. The spermogram after preparation and extration rates were compared using the Student's t test for paired variables and for three subpopulations identified by increasing spermatozoid concentrations.


RESULTS
Sperm count were significantly higher after percoll. Mobility and morphology were improved after centrifugation-migration excepting for the samples with the lowest counts. The extration rate was better after percoll and rose for the low-count samples.


CONCLUSIONS
Percoll gradient is the most effective method, particularly for altered sperm. The number of mobile and normal forms can be improved with the centrifugation-migration technique.
Mawissa
D
D
Unité de Fertilité, Hôpital Universitaire Erasme (ULB), Bruxelles, Belgique.
Legreve
A
A
Ragon
Y
Y
Birmane
J
J
Englert
Y
Y
D002498:Centrifugation; D005069:Evaluation Studies as Topic; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods* / Q000592:standards; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013076:Sperm Count; D013081:Sperm Motility
Evaluation des méthodes de préparation du sperme pour la fécondation in vitro humaine.