Genotoxicity of human mammary lipid.
We tested the proposition that human mammary lipid contains mutagenic/genotoxic agents that could cause DNA damage in adjacent epithelial cells. Lipid samples from breast tissue surgically removed from 40 women undergoing elective reduction mammoplasty were extracted by a solid-phase procedure. Mutagenicity was observed in Salmonella typhimurium TA98 and TA1538 in 16 of 40 (40%) extracts assayed with rat-liver S9, but not in its absence. No mutagenicity was seen in S. typhimurium TA100 or Escherichia coli WP2uvrA(pKM101). Bacterial mutagenicity correlated with micronucleus-forming activity in a metabolically competent mammalian cell line (MCL-5). This genotoxic activity merits further investigation in relation to the etiology of breast cancer.
Martin
F L
FL
Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, United Kingdom. FLMartin@icr.ac.uk
Carmichael
P L
PL
Crofton-Sleigh
C
C
Venitt
S
S
Phillips
D H
DH
Grover
P L
PL
D000273:Adipose Tissue / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D000818:Animals; D001711:Biotransformation; D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000209:etiology*; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics / Q000633:toxicity*; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000302:isolation & purification / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000494:pharmacology; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000378:metabolism; D008875:Middle Aged; D009152:Mutagenicity Tests; D009821:Oils / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D012995:Solubility
D002274:Carcinogens, Environmental; D008055:Lipids; D009821:Oils; D008315:Malondialdehyde