Reserpine-induced increases in neuropeptide Y mRNA of guinea pig sympathetic ganglia using in situ hybridization.
BACKGROUND
Neuropeptide Y (NPY) is synthesized in sympathetic ganglia by specific mRNA, to which rat probes are currently available. In the rat model, reserpine treatment increases NPY mRNA through a mechanism involving enhanced preganglionic activity. Probes for NPY mRNA have been used exclusively in rat models. In this study, we assessed whether a rat NPY cRNA probe could be used to index reserpine-induced changes in NPY mRNA levels of sympathetic ganglia in the guinea pig.


METHODS
Guinea pigs were given vehicle or reserpine pretreatment. In situ hybridization for NPY mRNA was done on the superior cervical and stellate ganglia of four control and four reserpine-treated rats. Autoradiographic density was digitized using an automated image analysis system.


RESULTS
Following in situ hybridization of tissue sections, autoradiographic density of specific NPY mRNA binding was evident in nerve cell bodies in the superior cervical and stellate ganglia. Reserpine pretreatment was associated with an increase in NPY mRNA levels in both types of ganglia.


CONCLUSIONS
These results indicate that reserpine treatment in the guinea pig produces increased neuronal NPY mRNA levels. The study also showed that rat NPY cRNA probe can be used to quantify alterations in NPY mRNA levels in the guinea pig.
Schmidt
E A
EA
Department of Anatomy, West Virginia University, Morgantown 26506, USA.
Huffman
L J
LJ
Dey
R D
RD
D000818:Animals; D015152:Blotting, Northern; D006168:Guinea Pigs; D017403:In Situ Hybridization; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012110:Reserpine / Q000494:pharmacology*; D013045:Species Specificity; D013233:Stellate Ganglion / Q000378:metabolism*; D017783:Superior Cervical Ganglion / Q000378:metabolism*
D009478:Neuropeptide Y; D012333:RNA, Messenger; D012110:Reserpine