Solamargine purified from Solanum incanum Chinese herb triggers gene expression of human TNFR I which may lead to cell apoptosis.
Solamargine possessed a potent cytotoxicity to human hepatocyte (Hep3B) and normal skin fibroblast. The inhibition curves of solamargine to the both cells were essentially overlapped, suggesting a parallel effect for the cell death. To define mechanism of cytotoxicity of solamargine, the changes of morphology and DNA content in cells were studied. A sub-G1 cell stage was drastically increased after 3-h incubation with solamargine. The results evidence that solamargine arises cell death by apoptosis. In addition, the gene expression of TNFR I were up-regulated within 30 min of solamargine treatment. Since TNF Receptor I has been involved in apoptosis, the overexpression of TNF receptor I may be related with the mechanism of cytotoxicity of solamargine. This communication is the first report that a component of Chinese herbs triggers gene expression of human TNFR I which may lead to cell apoptosis.
Hsu
S H
SH
Department of Biochemistry, Kaohsiung Medical College, Taiwan.
Tsai
T R
TR
Lin
C N
CN
Yen
M H
MH
Kuo
K W
KW
D015703:Antigens, CD / Q000096:biosynthesis*; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D010840:Phytosterols / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis*; D047888:Receptors, Tumor Necrosis Factor, Type I; D012867:Skin / Q000166:cytology; D012991:Solanaceous Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*
D015703:Antigens, CD; D004365:Drugs, Chinese Herbal; D010840:Phytosterols; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D012991:Solanaceous Alkaloids; C026609:beta-solamarine