A bioluminescent gamma-aminobutyrate assay for monitoring its release from inhibitory nerve endings.
A bioluminescent GABA assay is described. The principle of the procedure is based on the action of GABASE (GABA-aminotransferase plus succinic semialdehyde dehydrogenase), coupled to the detection of succinic semialdehyde and NADH, using Photobacterium luciferase. The method was used for monitoring GABA release from depolarized brain slices.
Israël
M
M
Laboratoire de Neurobologie Cellulaire et Moléculaire, CNRS, Gif sur Yvette, France.
Lesbats
B
B
D000818:Animals; D002531:Cerebellum / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D008163:Luminescent Measurements; D051379:Mice; D009411:Nerve Endings / Q000737:chemistry* / Q000378:metabolism; D009924:Organ Culture Techniques; D010776:Photobacterium; D011551:Pseudomonas fluorescens; D014733:Vibrio; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000378:metabolism
D005680:gamma-Aminobutyric Acid