Enzymic determinations with rotating bioreactors and continuous-flow-stopped-flow processing. Determination of choline esters in pharmaceuticals.
The neuromuscular blocking agent, succinylcholine, and the neurotransmitter, acetylcholine, have been determined in pharmaceutical preparations utilizing a sensing unit comprised of a rotating bioreactor and an amperometric detector. The bioreactor consisted of a rotating disk made of Teflon (1 cm id) with cholinesterase (EC 3.1.1.8) and choline oxidase (EC 1.1.3.17) co-immobilized on the top surface of the disk. Amperometric detection of H2O2 and initial rate measurements were employed for quantification.
López Ruiz
B
B
Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Sánchez-Cabezudo
M
M
Mottola
H A
HA
D000109:Acetylcholine / Q000032:analysis*; D000429:Alcohol Oxidoreductases; D019149:Bioreactors; D002802:Cholinesterases; D004800:Enzymes, Immobilized; D004364:Pharmaceutical Preparations / Q000032:analysis; D013390:Succinylcholine / Q000032:analysis*
D004800:Enzymes, Immobilized; D004364:Pharmaceutical Preparations; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002802:Cholinesterases; D013390:Succinylcholine; D000109:Acetylcholine