Effects of resistant starch- and vitamin A-supplemented diets on the promotion of precursor lesions of colon cancer in rats.
We have evaluated the potential protective effect of resistant starch (RS)- and vitamin A-supplemented diets on the promotion of preneoplasic lesions of rat colon, aberrant crypt foci (ACF), induced by 1,2-dimethylhydrazine dihydrochloride (DMH). We have tried to show whether the association of these two dietary constituents in the same diet could have synergistic effects. RS, vitamin A, and RS+ vitamin A were incorporated into the rat diets. Experimental diets were given one week after DMH injection and maintained for 12 weeks until the animals were sacrificed. The total number of ACF decreased with the three experimental diets. For RS- and RS + vitamin A-supplemented diets, this decrease is primarily due to a decrease in small ACF. For the vitamin A-supplemented diet, small and large ACF have a tendency to decrease. The effects of the diets on parameters influencing colon carcinogenesis were also studied. Only RS- and RS + vitamin A-supplemented diets have modified cecal pH, fecal and cecal butyrate contents, fecal excretion, cecal weight, and colon length. Vitamin A has been observed in colonic epithelial cells of rats receiving vitamin A- and RS+ vitamin A-supplemented diets. The association between RS and vitamin A shows neither a cumulative nor a synergistic protective effect.
Cassand
P
P
Laboratoire de Toxicologie Alimentaire, Université Bordeaux I, Talence, France.
Maziere
S
S
Champ
M
M
Meflah
K
K
Bornet
F
F
Narbonne
J F
JF
D000818:Animals; D002087:Butyrates / Q000378:metabolism; D002273:Carcinogens / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000453:epidemiology / Q000473:pathology* / Q000517:prevention & control*; D004357:Drug Synergism; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005527:Food, Fortified; D006863:Hydrogen-Ion Concentration; D015994:Incidence; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D011230:Precancerous Conditions / Q000473:pathology* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000494:pharmacology*; D014801:Vitamin A / Q000032:analysis / Q000494:pharmacology*
D002087:Butyrates; D002273:Carcinogens; D005232:Fatty Acids, Volatile; D014801:Vitamin A; D013213:Starch