Identification of cytokeratin subspecies altered in rat experimental esophageal tumors by subtractive cloning.
By using the subtractive hybridization method, two complementary DNA clones differently expressed in rat normal esophageal epithelium and squamous cell carcinoma induced by administration of precursors of N-nitrososarcosine ethyl ester were isolated. A rat homologue of the human 50-kDa type I cytokeratin 14 was cloned for the first time and shown to be expressed preferentially in squamous cell papillomas and carcinomas, whereas it was weakly expressed or absent in normal squamous epithelial cells and in hyperplastic lesions. A rat homologue of the mouse 57-kDa type II cytokeratin showed strong expression in both normal and tumor tissues. These results are well consistent with the reported alteration of keratin subspecies in human esophageal cancers, therefore, encouraging us to use this experimental system as a model for human esophageal carcinogenesis.
Wang
D Y
DY
First Department of Pathology, Hamamatsu University School of Medicine, Japan.
Xiang
Y Y
YY
Tanaka
M
M
Shen
Q
Q
Sugimura
H
H
D000818:Animals; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004935:Esophageal Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006965:Hyperplasia; D017403:In Situ Hybridization; D007633:Keratins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D009602:Nitrosamines; D010212:Papilloma / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000032:analysis; D013382:Subtraction Technique
D002273:Carcinogens; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009602:Nitrosamines; D011994:Recombinant Proteins; C028099:N-nitrososarcosine ethyl ester; D007633:Keratins