Activity of liver cytochrome P-450-dependent monooxygenase system in morphine-dependent rats.
Young female Wistar rats were injected intraperitoneally with increasing doses of morphine (9-45 mg/kg b.w. twice a day) for 14 days. The activities of the cytochrome P-450-dependent monooxygenase system and microsomal electron transport chain I were determined. Long-term morphine treatment decreased significantly cytochrome P-450 content, while it did not affect microsomal electron transport chain I. A decrease in cytochrome b5 content was accompanied by a compensatory increase in the activity of NADH-cytochrome b5 reductase. Both 4-aminopyrine N-demethylase activity and aniline hydroxylase activity decreased after morphine treatment (48% and 70%, respectively, when compared with controls).
Glogowska-Szelag
J
J
2nd Department of Histology and Embryology, Silesian School of Medicine, Katowice-Ligota, Poland.
Plewka
A
A
Kamiński
M
M
Górski
J
J
Kajdaniuk
D
D
Nowak
M
M
Buntner
B
B
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D009021:Morphine Dependence; D051381:Rats
D003577:Cytochrome P-450 Enzyme System