title: Conformational changes of the yeast mitochondrial adenosine diphosphate/adenosine triphosphate carrier studied through its intrinsic fluorescence. 2. Assignment of tryptophanyl residues of the carrier to the responses to specific ligands.
abstract: Tryptophanyl substitution of the Saccharomyces cerevisiae adenine nucleotide carrier (Anc2p isoform) was not deleterious for the transport activity or the folding of the carrier [preceding paper by Le Saux et al. (1996) Biochemistry 35, 16116-16124]. Conformational changes of the isolated wild-type and Trp-substituted Anc2p variants, induced upon binding of specific substrates [adenosine triphosphate (ATP) or diphosphate (ADP)] or inhibitors [carboxyatractyloside (CATR) or bongkrekic acid (BA)], were studied by measurement of intrinsic fluorescence. Titration of CATR and BA binding sites ended in the same number of sites, namely, 6-7 nmol/mg of wild-type and variant Anc2p. Isolated Anc2p in detergent presented similar emission spectra, suggesting that all tryptophanyl residues were in environments of similar hydrophobicity. Trp87 and Trp126 contributed largely and to a similar extent to the fluorescence enhancement observed in response to ATP binding, while Trp235 contributed negatively and to a small extent to the fluorescence change. Both Trp126 and Trp235, and to a lower extent Trp87, participate in the CATR-induced fluorescence decrease of Anc2p. Responses to BA binding were observed only in the presence of ATP; they consisted of a further fluorescence increase of the Anc2p.ATP complex, which was mainly due to Trp87 and Trp126, Trp235 being much less responsive. The different fluorescence responses of the three Trp residues of Anc2 variants to ATP, CATR, and BA are in agreement with distinct binding sites for these ligands and distinct conformations of the carrier protein recognizing specifically CATR or BA. A mechanistic model is proposed to interpret the transitions between the different conformational states of Anc2p.
authors.affiliation: Centre National de la Recherche Scientifique, Départment de Biologie Moléculaire et Structurale, CEA-Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Roux
authors.forename: A
authors.initials: A
authors.lastname: Le Saux
authors.forename: V
authors.initials: V
authors.lastname: Trézéguet
authors.forename: C
authors.initials: C
authors.lastname: Fiore
authors.forename: C
authors.initials: C
authors.lastname: Schwimmer
authors.forename: A C
authors.initials: AC
authors.lastname: Dianoux
authors.forename: P V
authors.initials: PV
authors.lastname: Vignais
authors.forename: G J
authors.initials: GJ
authors.lastname: Lauquin
authors.forename: G
authors.initials: G
authors.lastname: Brandolin
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D001278:Atractyloside / Q000031:analogs & derivatives / Q000378:metabolism; D001665:Binding Sites; D001865:Bongkrekic Acid / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000378:metabolism*; D008961:Models, Structural; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan
chemical_list: D011994:Recombinant Proteins; D001865:Bongkrekic Acid; D001278:Atractyloside; D000244:Adenosine Diphosphate; D014364:Tryptophan; D000226:Mitochondrial ADP, ATP Translocases; C003853:carboxyatractyloside