Analytical SEM of cell polyanions stained with tris(2,2'-bipyridine)ruthenium(II).
Peritoneal mast cells and lymphocytes from mice were placed on graphite supports, fixed in methanol, stained with the new fluorochrome tris(2,2'-bipyridine)ruthenium(II) and microanalysed using scanning electron microscopy (SEM). X-ray microanalysis showed the expected signal of P and S (K alpha lines) in emission spectra of lymphocytes and mast cells. The signal of Ru (L alpha 1 and L beta 1) overlapped with that of Cl, although the peak in the corresponding region was about 7 times higher than that from unstained cells. X-ray images showed the topographic localization of P, S and Ru in mast cells and lymphocytes and confirmed the accumulation of the Ru complex in heparin- and DNA-containing structures. These results indicate that, by using suitable marker elements and detection methods, analytical SEM is a useful complement in cytochemical studies.
Stockert
J C
JC
Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Spain.
Bertolesi
G E
GE
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives*; D000818:Animals; D002712:Chlorides / Q000032:analysis*; D004396:Coloring Agents; D056831:Coordination Complexes; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis*; D013455:Sulfur / Q000032:analysis*; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D002712:Chlorides; D004396:Coloring Agents; D056831:Coordination Complexes; D010758:Phosphorus; D013455:Sulfur; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds