Molecular cloning and expression of a novel peptide (LN1) gene: reduced expression in the renal cortex of lupus nephritis in MRL/lpr mouse.
A gene has been identified by mRNA differential display whose expression is reduced in the renal cortex of MRL/lpr mouse. The nucleotide sequence of the cDNA contains an open reading frame that encodes a protein of 338 amino acids (termed LN1). In situ hybridization showed that LN1 mRNA is present in glomeruli, and a 39 kDa protein was detected in the kidney by immunoblot. A human LN1 cDNA was also isolated, the deduced amino acid sequence of which is 78% identical to that of mouse LN1. Although the function of LN1 remains to be elucidated, its reduced expression may contribute to the pathogenesis of lupus nephritis.
Iwano
M
M
First Department of Internal Medicine, Nara Medical University, Japan.
Ueno
S
S
Miyazaki
M
M
Harada
T
T
Nagai
Y
Y
Hirano
M
M
Dohi
Y
Y
Akai
Y
Y
Kurioka
H
H
Dohi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007672:Kidney Cortex / Q000737:chemistry*; D007678:Kidney Glomerulus / Q000737:chemistry*; D008181:Lupus Nephritis / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019463:Mice, Inbred MRL lpr; D008969:Molecular Sequence Data; D010940:Plant Proteins; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013997:Time Factors; D014018:Tissue Distribution
D018398:Homeodomain Proteins; C095848:Kn1 protein, plant; D010940:Plant Proteins; D012333:RNA, Messenger