title: Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.
abstract: We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
authors.affiliation: Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St. Paul 55108, USA.
authors.forename: D
authors.initials: D
authors.lastname: Cue
authors.forename: H
authors.initials: H
authors.lastname: Lam
authors.forename: R S
authors.initials: RS
authors.lastname: Hanson
authors.forename: M C
authors.initials: MC
authors.lastname: Flickinger
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D001483:Base Sequence; D015342:DNA Probes; D015280:DNA Restriction-Modification Enzymes / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015342:DNA Probes; D015280:DNA Restriction-Modification Enzymes