Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II.
Cyclic guanosine 3',5'-monophosphate (cGMP)-dependent protein kinases (cGKs) mediate cellular signaling induced by nitric oxide and cGMP. Mice deficient in the type II cGK were resistant to Escherichia coli STa, an enterotoxin that stimulates cGMP accumulation and intestinal fluid secretion. The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes.
Pfeifer
A
A
Institut f-ur Pharmakologie und Toxikologie, Technische Universität München, Biedersteiner Strasse 29, D-80802 M-unchen, Germany. pfeifer@ipt.med.tu-muenchen.de
Aszódi
A
A
Seidler
U
U
Ruth
P
P
Hofmann
F
F
Fässler
R
R
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D001834:Body Water / Q000378:metabolism; D001846:Bone Development; D003433:Crosses, Genetic; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D003967:Diarrhea / Q000503:physiopathology; D004392:Dwarfism / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004768:Enterotoxins / Q000633:toxicity; D029968:Escherichia coli Proteins; D005260:Female; D017353:Gene Deletion; D006132:Growth Plate / Q000201:enzymology / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D015398:Signal Transduction
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); D015124:8-Bromo Cyclic Adenosine Monophosphate; C016276:8-bromocyclic GMP; D017869:Cyclic GMP-Dependent Protein Kinases; D006152:Cyclic GMP