Exon mapping by fiber-FISH or LR-PCR.
In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of >/=200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.
Florijn
R J
RJ
Department of Cytochemistry and Cytometry, State University Leiden, Wassenaarseweg 72, Leiden, 2333 AL, The Netherlands.
van de Rijke
F M
FM
Vrolijk
H
H
Blonden
L A
LA
Hofker
M H
MH
den Dunnen
J T
JT
Tanke
H J
HJ
van Ommen
G J
GJ
Raap
A K
AK
D002874:Chromosome Mapping / Q000379:methods*; D003360:Cosmids / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000648:ultrastructure; D004274:DNA, Recombinant / Q000235:genetics* / Q000648:ultrastructure; D016189:Dystrophin / Q000235:genetics; D005091:Exons / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D009136:Muscular Dystrophies / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites
D018076:DNA, Complementary; D004274:DNA, Recombinant; D016189:Dystrophin