title: Flow cytometric DNA analysis of gastric cancer that is invading the muscularis propria.
abstract: OBJECTIVE
  To find out if there is any correlation between the DNA aneuploidy in gastric cancer that is invading the muscularis propria and survival.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Teaching hospital, Japan.
  
  
  METHODS
  52 patients who underwent gastric resection for gastric cancer that was invading the muscularis propria, and for whom clinical follow-up data were available.
  
  
  METHODS
  Flow cytometric measurement of DNA in paraffin-embedded tissue.
  
  
  METHODS
  Correlation between survival and clinicopathological variables including ploidy.
  
  
  RESULTS
  22 (42%) of the cases were aneuploid and 30 (58%) were diploid. The lymph node status and DNA ploidy were the significant prognostic variables, but a multivariate analysis showed that only DNA ploidy was significant.
  
  
  CONCLUSIONS
  Nuclear DNA ploidy is an independent prognostic factor in gastric cancer that is invading the muscularis propria.
authors.affiliation: Department of Surgery II, Fukushima Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizunuma
authors.forename: A
authors.initials: A
authors.lastname: Tsuchiya
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: R
authors.initials: R
authors.lastname: Abe
mesh_terms: D000782:Aneuploidy; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D016612:Paraffin Embedding; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm