Antimutagenic activity of extracts from Japanese eggplant.
Using the Salmonella/microsome assay, the antimutagenic effects of specific components of the extracts from eggplant fruits were investigated. The eggplant fruit juice exhibited an antimutagenic activity against 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) induced mutagenicity. In some of the fractions extracted with several organic solvents (acetone, petroleum ether, ethyl acetate; and methanol), the activity was recognized. No mutagenicity or toxicity for Salmonella typhimurium TA98 in the presence of S9 mixture was observed with any of the extracts. It is suggested that there are multiple components of the activities that exist in the eggplant fruit. We isolated lutein from the 84% methanol (methanol/water, v/v) layer, pheophorbide or chlorophyllide from the 70% methanol layer and tannins containing sugar-moieties from the water layer. Pheophytin a and b, Mg-free derivatives of chlorophyll a and b, were isolated from the petroleum ether layer as possible antimutagens. The pheophytin a with S9 mix inhibited by 30-40% the mutagenicity of Trp-P-2.
Yoshikawa
K
K
Department of Food and Nutrition, Kinki University, Nara, Japan.
Inagaki
K
K
Terashita
T
T
Shishiyama
J
J
Kuo
S
S
Shankel
D M
DM
D016587:Antimutagenic Agents / Q000494:pharmacology*; D002243:Carbolines / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000235:genetics; D014675:Vegetables / Q000737:chemistry*
D016587:Antimutagenic Agents; D002243:Carbolines; D009153:Mutagens; D010936:Plant Extracts; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole