title: A porcine model of peritonitis and bacteremia simulates human septic shock.
abstract: Cardiovascular responses to systemic bacteremia were evaluated in a pre-instrumented, conscious pigs. Basal observations were obtained 5-7 days after instrumentation. On the next day, Escherichia coli 0111.B4 (1.1 to 33 x 10(9) CFU/kg)-laden fibrin clots were implanted intraperitoneally. Nonsurvivors (9/18) demonstrated rapid cardiovascular decompensation. Survivors (9/18) demonstrated significant cardiovascular injury, which was reversed by 5-7 days postimplant. Cardiac inotropicity was significantly reduced in this period, but recovered by day 7. Circulating myocardial depressant substance activity (assayed by serum-induced depression of beating neonatal rat myocytes) was present on days 1-4 of bacteremia and recovered to basal values on day 6. No clinical or cardiovascular changes were seen in pigs implanted with sterile clots (n = 4). These data demonstrate that implantation of bacteria-laden fibrin clots in pigs induces cardiovascular alterations that mimic responses seen in human sepsis.
authors.affiliation: Section of Cardiology, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Goldfarb
authors.forename: D
authors.initials: D
authors.lastname: Glock
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: R J
authors.initials: RJ
authors.lastname: McCarthy
authors.forename: J
authors.initials: J
authors.lastname: Mei
authors.forename: T
authors.initials: T
authors.lastname: Guynn
authors.forename: M
authors.initials: M
authors.lastname: Matushek
authors.forename: G
authors.initials: G
authors.lastname: Trenholme
authors.forename: J E
authors.initials: JE
authors.lastname: Parrillo
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000150:complications / Q000401:mortality / Q000503:physiopathology*; D001794:Blood Pressure; D002302:Cardiac Output; D002319:Cardiovascular System / Q000382:microbiology / Q000503:physiopathology; D002478:Cells, Cultured; D003326:Coronary Circulation; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000472:pathogenicity; D005260:Female; D006339:Heart Rate; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D010538:Peritonitis / Q000150:complications / Q000503:physiopathology*; D051381:Rats; D012772:Shock, Septic / Q000503:physiopathology*; D015996:Survival Rate; D013552:Swine; D013997:Time Factors; D014180:Transplantation; D014655:Vascular Resistance