The possible role of C5 segment inspiratory interneurons investigated by cross-correlation with phrenic motoneurons in decerebrate cats.
We tested the role of C5 segment inspiratory interneurons in transcribing central respiratory drive to phrenic motoneurons and mediating intersegmental reflexes by cross-correlating the spontaneous activity of 26 interneurons with that of the ipsi -and contralateral C5 phrenic nerves in decerebrate cats. There were 10 interneurons that discharged only during inspiration (phrenic burst) and 16 that discharged tonically with increased firing during inspiration. Of the cross-correlograms for 26 of the interneurons with the ipsilateral phrenic, 20 were flat and 2 had peaks centred about time zero, interpreted as a common activation of the interneurons and motoneurons. The cross-correlograms for 4 other interneurons had troughs centred about time zero, interpreted as a synchronous excitation of the interneurons and inhibition of the motoneurons. Of the cross-correlograms for 23 interneurons with the contralateral phrenic, 22 were flat and 1 had a peak centred about time zero, interpreted as a common activation of the interneuron and motoneurons. Nine of ten cross-correlograms between pairs of interneurons were flat; one had a peak centred about time zero. We conclude that, despite their inspiratory modulated discharge patterns, there is no evidence from this study that the C5 segment inspiratory interneurons convey central respiratory drive to C5 phrenic motoneurons.
Duffin
J
J
Department of Physiology, University of Toronto, ON Canada. j.duffin@utoronto.ca
Iscoe
S
S
D000818:Animals; D002415:Cats; D003655:Decerebrate State; D004594:Electrophysiology; D005260:Female; D007395:Interneurons / Q000502:physiology*; D009046:Motor Neurons / Q000502:physiology*; D009333:Neck; D010791:Phrenic Nerve / Q000166:cytology / Q000502:physiology*; D012119:Respiration / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*