A lipid-anchored binding protein is a component of an ATP-dependent cellobiose/cellotriose-transport system from the cellulose degrader Streptomyces reticuli.
During cultivation in the presence of cellobiose or crystalline cellulose, Streptomyces reticuli expresses an inducible uptake system that transports cellobiose (K(m), 4 microM), cellotriose and, to a lesser degree, cellotetraose and cellopentaose. Cellobiose uptake is dependent on ATP and inhibited by N-ethylmaleimide. A binding protein was identified in its palmitylated form in the cytoplasmic membrane of mycelia. It could be extracted with the detergent Triton X-100 and purified by two subsequent anion-exchange chromatographies. It showed highest affinity (Kd, 1.5 microM) for cellobiose and cellotriose. The data suggest that cellobiose/cellotriose uptake is mediated by a membrane-anchored lipoprotein as a component of an ATP-binding-cassette-transporter system.
Schlösser
A
A
Universität Osnabrück, Germany.
Schrempf
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D002942:Circular Dichroism; D007700:Kinetics; D008074:Lipoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013302:Streptomyces / Q000378:metabolism*; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism*; D014364:Tryptophan
D008074:Lipoproteins; D014312:Trisaccharides; D002475:Cellobiose; C023872:mannotriose; D014364:Tryptophan; D000255:Adenosine Triphosphate; D002482:Cellulose