Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene.
We previously reported the cloning of a murine cDNA encoding the protein-serine/threonine kinase Akt2, and we used this clone to map the Akt2 gene to mouse chromosome (MMU) 7B1 by fluorescence in situ hybridization. We now have cloned and partially sequenced a mouse Akt2 pseudogene. An analysis of two sets of multilocus crosses revealed that the Akt2 gene is closely linked to the Cyp2a locus in proximal MMU7. The Akt2 pseudogene was mapped to proximal MMU11 by both multilocus mapping and fluorescence in situ hybridization.
Altomare
D A
DA
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Kozak
C A
CA
Sonoda
G
G
Testa
J R
JR
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D005796:Genes; D008040:Genetic Linkage; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009115:Muridae / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D011544:Pseudogenes
D018076:DNA, Complementary; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; C494922:Akt2 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt