title: Effect of Pasteurella multocida toxin on in vivo immune responses in piglets.
abstract: Atrophic rhinitis is characterized by a lack of an immune response towards Pasteurella multocida toxin (Pm-T). The effects of intranasal administration of Pm-T on cellular and T-cell dependent antibody responses of piglets against various other antigens were studied in a 3 by 2 factorial arrangement of treatments: three levels of challenge with Pm-T (either once; on three consecutive days; or no Pm-T challenge), and with or without simultaneous immunization with a "cocktail' containing Keyhole limpet haemocyanin (KLH), ovalbumin (OA) and tetanus toxoid (TT). Challenge with Pm-T affected but did not abolish the in vivo humoral immune response against OA, KLH, or TT. Pigs treated once with Pm-T showed lower total antibody responses, caused by lower IgG responses to the antigens. We found no clear consistent effects of Pm-T on cellular immune responses to the various T-cell-dependent antigens in vitro. The lack of immune responsiveness to Pm-T in Pm-T challenged pigs is discussed. The absence of detectable immune responses does not depend on a general suppression of the cellular immune system.
authors.affiliation: Wageningen Agricultural University, Department of Animal Husbandry, The Netherlands.
authors.forename: P M
authors.initials: PM
authors.lastname: van Diemen
authors.forename: G
authors.initials: G
authors.lastname: de Vries Reilingh
authors.forename: H K
authors.initials: HK
authors.lastname: Parmentier
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D007075:Immunoglobulin M / Q000097:blood / Q000378:metabolism; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D016979:Pasteurella multocida / Q000276:immunology; D011897:Random Allocation; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013745:Tetanus Toxoid / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C062258:Pasteurella multocida toxin; D013745:Tetanus Toxoid; D010047:Ovalbumin; D006433:Hemocyanins