title: The expression of cytokines by an established basal cell carcinoma cell line (BCC-1/KMC) compared with cultured normal keratinocytes.
abstract: A basal cell carcinoma (BCC) cell line (BCC-1/KMC) has recently been successfully established from a patient. The production of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6 and IL-8 was assessed in comparison with that of cultured normal keratinocytes. The mRNA expression of these cytokines was measured by a reverse transcriptase-polymerase chain reaction (RT-PCR) method and the protein production by an ELISA. The cultured BCC cells spontaneously secreted more IL-6 and IL-8 but less IL-1 than the keratinocytes after culture for 24 h at 37 degrees C. It is suggested that the increased expression of IL-6 and IL-8 may indicate the transformation of normal keratinocytes to locally aggressive BCC.
authors.affiliation: Graduate Institute of Medical Sciences, Kaohsiung Medical College, Taiwan, ROC.
authors.forename: H T
authors.initials: HT
authors.lastname: Yen
authors.forename: L C
authors.initials: LC
authors.lastname: Chiang
authors.forename: K H
authors.initials: KH
authors.lastname: Wen
authors.forename: C C
authors.initials: CC
authors.lastname: Tsai
authors.forename: C L
authors.initials: CL
authors.lastname: Yu
authors.forename: H S
authors.initials: HS
authors.lastname: Yu
mesh_terms: D002280:Basal Cell Carcinoma / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012016:Reference Values; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines