Structure of the rat collagen IV promoter.
We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the alpha 1 (IV) and alpha 2 (IV) collagen chains. There are at least two transcription start sites within both the alpha 1 (IV) and alpha 2 (IV) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the alpha 1 (IV) and alpha 2 (IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the alpha 2 (IV) direction than in the alpha 1 (IV) direction.
Grande
J P
JP
Department of Laboratory Medicine and Pathology, Mayo Graduate School of Medicine, Rochester, MN 55905, USA.
Melder
D C
DC
Kluge
D L
DL
Wieben
E D
ED
D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005796:Genes / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005920:Glomerular Mesangium / Q000166:cytology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
D011993:Recombinant Fusion Proteins; D003094:Collagen; D015500:Chloramphenicol O-Acetyltransferase