In situ methylation of nucleic acids using pyrolysis/mass spectrometry.
Curie-point pyrolysis/triple quadrupole mass spectrometry (Py/MS/MS) has been used with tetramethylammonium hydroxide (TMAH) to conduct in situ methylation of nucleic acid bases. Nitrogen bases in free nucleotides, oligonucleotides, calf thymus DNA and whole bacterial cells reacted in situ during pyrolysis with TMAH to form the methylated bases. Derivatization increased the volatility of the nitrogen bases and the mass of the diagnostic base peaks, thereby removing them from the positions of lower-mass background peaks. The degree of methylation as a function of TMAH concentration for the oligonucleotide, calf thymus DNA, and the whole bacteria samples was determined and found to correlate with the nature of DNA. The methylated bases were identified by their positive-ion electron ionization fragmentation patterns and confirmed with tandem mass spectrometry. The detection of the methylated bases by Py/MS/MS facilitates the goal of identifying the nucleic acids in a complex mixture (i.e. whole bacterial cells) without extraction and prior derivatization.
Abbas-Hawks
C
C
Department of Chemistry, Colorado School of Mines, Golden 80401-1887, USA.
Voorhees
K J
KJ
Hadfield
T L
TL
D000818:Animals; D015415:Biomarkers; D002002:Brucella / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry; D019175:DNA Methylation; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009711:Nucleotides / Q000032:analysis; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D015415:Biomarkers; D007202:Indicators and Reagents; D009711:Nucleotides; D009841:Oligonucleotides; D004247:DNA