[Studies on murine model of nasal allergy].
For the purpose of making a murine model of nasal allergy, we performed the ovalbumin (OA)-sensitization on groups of BALB/c mice in four different ways. The 1st group was sensitized with OA + alum by introperitoneal myection on day 1, 5 and 21, and the 2nd group was added OA nasal drips on day 28, 34. The 3rd group was performed OA + alum nasal dripping on day 1, 5 and 21, and the 4th was a added OA nasal dripping in the same way with the 2nd group. The observed OA-induced nasal symptoms, mucosal eosinophillia and 8 days-PCA titer were remarkable in 2nd group, moreover it also showed higher histamine hypersensitivity than other groups. We concluded that mice sensitized by OA with alum are useful for research of nasal allergy, for the reason that they posses three features of nasal allergy, anti-intigen-induced nasal symptoms, eosinophils infiltration and antigen-specific PCA titer as index of IgE increasing.
Watanabe
M
M
Department of Otolaryngology School of Medicine, Sapporo Medical University.
Asakura
K
K
Saito
H
H
Kataura
A
A
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009668:Nose Diseases / Q000209:etiology* / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000209:etiology* / Q000276:immunology
D000941:Antigens