Effects of interleukin-2 and cyclosporin A on pathologic features in Mycoplasma pneumonia.
To elucidate the immunopathologic mechanisms of Mycoplasma pneumonia, the effects of interleukin-2 (IL-2) and cyclosporin A (CYA) on Mycoplasma pneumonia in mice were investigated. Mice were intranasally inoculated with Mycoplasma pulmonis (M. pul) and treated with IL-2, CYA, or minocycline (MINO) every day between Days 3 and 9. They were killed at Days 7, 14, or 21 after the inoculation. Cell-mediated immunity (CMI) of the host was assessed by delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC). Peribronchial and perivascular lymphocyte cuffing and the accumulation of macrophages at the ends of bronchioles were exacerbated (p < 0.05) in IL-2-treated mice at Day 14 and reduced (p < 0.05) in CYA-treated mice at Day 7. Although the DTH responses to SRBC of saline-inoculated, IL-2-treated mice were increased at Days 7 (p < 0.01) and 14 (p < 0.05), those of M. pul-inoculated, IL-2-treated mice at Day 7 could not recover to the control level. The CMI levels of M. pul-inoculated, CYA-treated mice were decreased at Days 7, 14 (p < 0.05), and 21 (p < 0.01). Mycoplasma organisms in the lung showed the greatest decrease (p < 0.05) in MINO- and IL-2-treated mice, but increased at Day 21 (p < 0.05) in mice treated with IL-2 alone. These results suggest that the pathologic features of Mycoplasma pneumonia could be modified by the degree of host CMI.
Tanaka
H
H
Third Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
Honma
S
S
Abe
S
S
Tamura
H
H
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000494:pharmacology*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008813:Mice, Inbred ICR; D008911:Minocycline / Q000494:pharmacology; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000276:immunology / Q000473:pathology*
D007376:Interleukin-2; D016572:Cyclosporine; D008911:Minocycline