title: Improved method for study of chromosomes of chorionic villus samples.
abstract: Chorionic villus sampling (CVS) offers rapid prenatal diagnosis of chromosomal disorders. We evaluated four methods for chromosomal analysis of chorionic villi (three based on direct preparations and one on long-term culture) in order to define a method which would provide good quality metaphases in sufficient numbers. The direct culturing method using synchronisation and dissociation with dispase was as good as long-term culturing. The optimal period of gestation for study was 10-12 wk.
authors.affiliation: Department of Paediatrics, WHO Collaborating Centre in Genetics All India Institute of Medical Sciences, New Delhi.
authors.forename: M R
authors.initials: MR
authors.lastname: Chowdhury
authors.forename: R
authors.initials: R
authors.lastname: Mathur
authors.forename: K
authors.initials: K
authors.lastname: Prabhakara
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Dubey
authors.forename: I C
authors.initials: IC
authors.lastname: Verma
mesh_terms: D015193:Chorionic Villi Sampling; D002874:Chromosome Mapping; D003582:Cytogenetics / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D011247:Pregnancy; D013997:Time Factors