Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence.
For 275 samples tested for herpes simplex virus (HSV), cytospin-enhanced direct immunofluorescence using Chemicon HSV monoclonal antibodies identified 80 (95%) and culture identified 77 (92%) of 84 confirmed positive specimens. Cytospin-prepared slides contained a greater number of total cells than standard cell spots, resulting in fewer inadequate cell smears and a higher HSV detection rate.
Landry
M L
ML
Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut, USA.
Ferguson
D
D
Wlochowski
J
J
D002498:Centrifugation; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D018139:Simplexvirus / Q000302:isolation & purification*