title: Expression of cell adhesion molecules and common acute lymphoblastic leukaemia antigen in hepatoblastoma.
abstract: Hepatoblastoma is an embryonal tumour of the liver, which often contains tissue components with multidirectional differentiation. The occurrence of cell surface antigens in this tumour has not been studied systematically, and we therefore investigated 20 hepatoblastomas for the expression of common acute lymphoblastic leukaemia antigen (CALLA) and cell adhesion molecules (CAMs) in their different tissue components. Epithelial tumour cells of fetal differentiation contained E-cadherin. This protein did not occur in tumour areas with embryonal or mesenchymal differentiation. In contrast, immature embryonal and anaplastic cells expressed CALLA and the hyaluronate receptor (HCAM, CD44). Both fetal and embryonal areas stained irregularly positive for ICAM-1, which, in contrast, was not present on anaplastic cells. Immature fibrous tissue did not contain any of these molecules except for ICAM-1. However, some cells adjacent to, or enclosed in, osteoid were positive for HCAM and NCAM. Like small undifferentiated hepatoblastoma cells, primitive mesenchymal spindle-shaped cells also expressed CALLA, HCAM, and the polysialylated embryonic form of NCAM strongly. This last is not present on other epithelial or mesenchymal tumour cells. Hepatoblastoma cells of varying differentiation express distinct patterns of CAMs and CALLA. Our results give further insight into their histogenesis and cellular interactions and may explain their variable ability for invasive growth and formation of metastases.
authors.affiliation: Department of Paediatric Surgery, Medical School, University of Hannover, Germany.
authors.forename: D
authors.initials: D
authors.lastname: von Schweinitz
authors.forename: I
authors.initials: I
authors.lastname: Leuschner
authors.forename: S
authors.initials: S
authors.lastname: Glüer
authors.forename: T
authors.initials: T
authors.lastname: Pietsch
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D018197:Hepatoblastoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007223:Infant; D008113:Liver Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008297:Male
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD