title: Infrequent Ki-ras and an absence of p53 mutations in hepatocellular carcinomas induced by a choline deficient L-amino acid defined diet in rats.
abstract: Mutations of Ki-ras and p53 genes in hepatocellular carcinomas (HCCs) induced by the choline deficient L-amino acid defined (CDAA) diet in rats were investigated by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) analysis followed by direct sequencing. Male Fischer 344 rats, 6 weeks old, were continuously given a CDAA diet for 70 weeks and then sacrificed. Macroscopically detectable nodules which were histologically confirmed to be well-differentiated HCCs were dissected free from the surrounding tissue and subjected to gene mutation analysis along with samples of non-tumor areas. Conformational change in the Ki-ras gene was detected in 1 out of 7 HCCs, involving a GGC to GTC transversion at codon 13. No p53 mutations were detected in 7 HCCs and also neither Ki-ras nor p53 mutations were found in non-tumor areas. The results suggest that neither Ki-ras nor p53 genes play an important role in hepatocarcinogenesis caused by long term expose to a CDAA diet in rats.
authors.affiliation: Department of Oncological Pathology, Nara Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsujiuchi
authors.forename: A
authors.initials: A
authors.lastname: Kido
authors.forename: D
authors.initials: D
authors.lastname: Nakae
authors.forename: M
authors.initials: M
authors.lastname: Takahama
authors.forename: T
authors.initials: T
authors.lastname: Majima
authors.forename: K
authors.initials: K
authors.lastname: Kobitsu
authors.forename: E
authors.initials: E
authors.lastname: Okajima
authors.forename: M
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: A
authors.initials: A
authors.lastname: Denda
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D002796:Choline Deficiency / Q000150:complications*; D003062:Codon / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004032:Diet; D016158:Genes, p53; D011905:Genes, ras; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000209:etiology / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D051381:Rats
chemical_list: D000596:Amino Acids; D003062:Codon; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm