[Comparative study of serological markers for intolerance to gluten].
In France as well as in most of the other European countries, the prevalence of coeliac disease is underestimated. In order to point out a good screening test, we have determined the most sensitive combination (technique-marker) for the diagnosis of the disease among 81 individuals (50 with coeliac disease and 31 controls). Serum anti-gliadin antibodies were measured using three methods: the qualitative dot-blot (Gliastick-Eurospital) and two quantitative methods Elisa (homemade-Saint-Antoine Hospital and alpha-Gliatest-Eurospital); serum anti-endomysium antibodies (EmA) and anti-reticulin antibodies (ARA) were detected using an indirect immunofluorescence assay. We have shown that the simple and fast Gliastick test can fulfil the selected criterion with a sensitivity of 0.90. Nevertheless, uncertain and positives results have to be confirmed by one of the two more specific quantitative tests. The two other markers (ARA and EmA) have shown a better specificity (1) but they were less sensitive (0.54 and 0.56 for ARA and EmA respectively). Thus, they have both to be used as confirmation tests and for follow-up with supervision of the compliance to recommended diet. In conclusion, the Gliastick can be considered as a good screening test for the detection of anti-gliadin antibodies and it would represent the expected help to determine the prevalence of coeliac disease on a large-scale map.
Dubel
L
L
Laboratoire d'immunologie, hôpital Saint-Antoine, Paris, France.
Absalon
Y B
YB
Baudon
J J
JJ
Johanet
C
C
D000328:Adult; D000906:Antibodies / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D002446:Celiac Disease / Q000175:diagnosis* / Q000517:prevention & control*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005470:Fluorometry; D005903:Gliadin / Q000276:immunology; D006801:Humans; D008297:Male; D012155:Reticulin / Q000276:immunology
D000906:Antibodies; D001323:Autoantibodies; D015415:Biomarkers; D012155:Reticulin; D005903:Gliadin
Etude comparative des marqueurs sérologiques de l'intolérance au gluten.