99mTechnetium-stannous oxinate as marker of liposome formulations.
Liposomes associated with tin(II) dioxinate were prepared from egg yolk phosphatidylcholine and cholesterol as sterile and pyrogen-free multilamellar or unilamellar vesicles. Complexing of liposomal tin(II) dioxinate with 99mTc attained 98% of the added radioactivity. Thirty percent 99mTc were released during 24-h incubation in biological fluids. The absence of tin colloids seen by electron microscopy and the stability of liposomal phospholipid and tin(II) dioxinate during 72-h incubation at 37 degrees C in plasma and cerebrospinal fluid would allow safe and reliable scintigraphic liposome pharmacokinetic studies.
Alafandy
M
M
Department of Anesthesiology, Flemish Free University of Brussels Medical Center, Belgium.
Goffinet
G
G
Umbrain
V
V
D'Haese
J
J
Camu
F
F
Legros
F J
FJ
D002784:Cholesterol; D003102:Colloids; D004146:Dioxanes; D004337:Drug Carriers; D005613:Freeze Etching; D006801:Humans; D008081:Liposomes; D008244:Lysophosphatidylcholines; D008854:Microscopy, Electron; D015609:Organotechnetium Compounds / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D009947:Organotin Compounds / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D010713:Phosphatidylcholines; D011877:Radionuclide Imaging; D015203:Reproducibility of Results; D013997:Time Factors
D003102:Colloids; D004146:Dioxanes; D004337:Drug Carriers; D008081:Liposomes; D008244:Lysophosphatidylcholines; D015609:Organotechnetium Compounds; D009947:Organotin Compounds; D010713:Phosphatidylcholines; C105028:technetium Tc 99m stannous oxinate; C105027:tin(II) dioxinate; D002784:Cholesterol