Relationship between interleukin-8 and neutrophil adhesion molecules in rheumatoid arthritis.
To evaluate the role of interleukin-8 (IL-8) on the activation of neutrophils in rheumatoid arthritis (RA), we measured IL-8 and the adhesion molecules, L-selectin (CD62L), CD1 1b and CD18, on neutrophils in paired peripheral blood and synovial fluid of RA patients. Synovial fluid IL-8 levels were significantly increased compared to peripheral blood. L-selectin was split off and CD1 1b and CD 18 were upregulated on neutrophils in the synovial fluid. A positive correlation occurred between the IL-8 concentration and CD18 or CD1 1b densities on neutrophils in the synovial fluid (r = 0.75, P < 0.005 and r = 0.60, P < 0.05, respectively). Peripheral blood neutrophils of the patients were desensitised with IL-8 in vivo, as shown by the significantly lower L-selectin shedding after in vitro IL-8 stimulation: 1.6 times decrease for patients vs 3.2 for controls (P < 0.05). In conclusion, these results add further evidence for the role of IL-8 in the activation of neutrophils in RA.
De Gendt
C M
CM
Department of Immunology, Allergology and Rhematology, Universitaire instelling Antwerpen, Belgium.
De Clerck
L S
LS
Bridts
C H
CH
Van Osselaer
N
N
Stevens
W J
WJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood*; D018845:CD11 Antigens / Q000097:blood*; D018821:CD18 Antigens / Q000097:blood*; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D019041:L-Selectin / Q000097:blood*; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism
D018845:CD11 Antigens; D018821:CD18 Antigens; D016209:Interleukin-8; D019041:L-Selectin