title: Chlamydia trachomatis major outer membrane protein variants escape neutralization by both monoclonal antibodies and human immune sera.
abstract: We have identified two families of novel Chlamydia trachomatis isolates with amino acid changes within the major outer membrane protein (MOMP) variable domains: one family of Da, D*, and D- and one family of Ia and I-. In order to determine whether these MOMP variants can escape antibody neutralization of infectivity, we tested both the D and I prototype strains and the variants in a complement-independent in vitro neutralization assay. We found that variants can indeed escape neutralization by both monoclonal antibodies and polyclonal human immune sera that neutralize the prototype strain.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195, USA. lampe@u.washington.edu
authors.forename: M F
authors.initials: MF
authors.lastname: Lampe
authors.forename: K G
authors.initials: KG
authors.lastname: Wong
authors.forename: L M
authors.initials: LM
authors.lastname: Kuehl
authors.forename: W E
authors.initials: WE
authors.lastname: Stamm
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002690:Chlamydia Infections / Q000276:immunology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology*; D014644:Genetic Variation; D006801:Humans; D009500:Neutralization Tests; D018272:Porins
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis