title: Placental proopiomelanocortin gene expression, adrenocorticotropin tissue concentrations, and immunostaining increase throughout gestation and are unaffected by prostaglandins, antiprogestins, or labor.
abstract: The objective of this study was to demonstrate the ontogeny of POMC gene expression, the distribution of immunoreactive ACTH, and tissue peptide content within the placenta and fetal membranes and to investigate the regulatory effects of PGs and progesterone during the first trimester and of labor at term. Tissues were collected from the following groups: 1) women undergoing first trimester (gestation 5-12 weeks) therapeutic abortion (by suction curettage with and without the synthetic PGE1 analogue, gemeprost administered vaginally 2-4 h before the procedure or with 600 mg mifepristone 48 h before receiving 1 mg gemeprost vaginally); 2) women undergoing second trimester therapeutic abortion (600 mg mifepristone; 1 mg gemeprost); 3) in association with delivery at term by spontaneous labor; 4) induced labor; or 5) elective caesarean section. ACTH was immunolocalized to the placental cytotrophoblasts in the first trimester and to the syncytiotrophoblasts in the second and third trimester. The intensity of the staining increased with advancing gestation. ACTH immunoreactivity also was localized in the epithelial layer of the amnion, the reticular layer of the chorion, and the decidual stroma. ACTH content measured by RIA in placental extracts increased significantly in the third trimester. In situ hybridization demonstrated expression of POMC messenger RNA in syncytiotrophoblasts and cytotrophoblasts from the first trimester and also demonstrated a significant increase in POMC gene expression with advancing gestation. The localization and staining intensity for ACTH and POMC gene expression were not affected by the administration of PGs or mifepristone or by labor at term. These data demonstrate the localization of ACTH immunoreactivity within the placenta throughout pregnancy, supporting the hypothesis that the placenta may activate and maintain the maternal and/or fetal hypothalamo-pituitary-adrenal axis throughout pregnancy.
authors.affiliation: Medical Research Council Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, United Kingdom.
authors.forename: E S
authors.initials: ES
authors.lastname: Cooper
authors.forename: I A
authors.initials: IA
authors.lastname: Greer
authors.forename: A N
authors.initials: AN
authors.lastname: Brooks
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007743:Labor, Obstetric / Q000378:metabolism*; D015735:Mifepristone / Q000494:pharmacology; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D011453:Prostaglandins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D006727:Hormone Antagonists; D011453:Prostaglandins; D012333:RNA, Messenger; D015735:Mifepristone; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone