Identification of two rat genes orthologous to the human interleukin-8 receptors.
The genes encoding two functional human interleukin-8 (IL-8) receptors have been identified by molecular cloning techniques and they are members of the rhodopsin G-protein coupled receptor (GCR) superfamily. We report the molecular cloning of two rat GCR genes (rat CXCR1-like and rat CXCR2) whose conceptualized amino acid sequences are approximately 70% identical to the human IL-8 A and B receptor subtypes. The murine GRO-like peptide, macrophage inflammatory peptide-2 (MIP-2), elevates intracellular calcium levels in HEK293 cells expressing the rat CXCR2 receptor. Southern blot analysis of restriction-digested rodent and human genomic DNAs indicate that rat CXCR1-like and CXCR2 are: 1) each single copy genes in the rat genome; 2) most closely related to the human IL-8 receptor genes; and 3) orthologous to two previously identified murine genes. CXCR2 mRNA is detected in adult rat lung, spleen, and neutrophils. CXCR1-like mRNA can be detected in adult rat lung, native rat macrophages, and a rat alveolar macrophage cell line (NR8383). These data identify the rat orthologs of the human IL-8 receptors, and describe cellular and tissue targets of rat C-X-C chemokine peptides.
Dunstan
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CA
Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, Florida 32610-0267, USA. jharrison@qm.server.ufl.edu
Salafranca
M N
MN
Adhikari
S
S
Xia
Y
Y
Feng
L
L
Harrison
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D019742:Chemokines, CC; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D005796:Genes; D006801:Humans; D017403:In Situ Hybridization; D019402:Macrophage Inflammatory Proteins; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D018121:Receptors, Cytokine / Q000235:genetics* / Q000502:physiology; D018123:Receptors, Interleukin / Q000235:genetics* / Q000502:physiology; D023062:Receptors, Interleukin-8A; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution
D015703:Antigens, CD; C095218:Ccl9 protein, mouse; D019742:Chemokines, CC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D002118:Calcium