title: Vaccination with fixed feline immunodeficiency virus (FIV) infected cells: protection, breakthrough and specificity of response.
abstract: Infection of cats with feline immunodeficiency virus (FIV), a naturally occurring lentivirus infection of cats which causes an AIDS-like disease, has generated considerable interest as an animal model for HIV vaccination. This paper reports on experiments performed to examine the potential of a fixed infected cell vaccine to confer protection against intraperitoneal challenge with cell-free FIV. The cell vaccine was highly immunogenic and elicited antibody responses to virus core antigen, p24, high virus neutralizing (VN) antibody titres, and antibodies which recognized cellular components of the vaccine. Whilst protection, assessed by the inability to detect infectious virus by virus isolation or polymerase chain reaction, against homologous but not heterologous FIV isolates was apparent up to week 12 post-challenge, when cats were monitored longer up to week 50 post-challenge a breakthrough in vaccine protection against homologous virus was observed. Protection could not be correlated with levels of antibody to p24 or VN antibody titres. In contrast with simian immunodeficiency virus vaccine studies in macaques there was no clear evidence that antibodies recognizing cellular components of the vaccine, including MHC class I and II antigens, conferred any protective effect following challenge. These results indicate that long-term post-challenge monitoring for infection is essential in lentivirus vaccine trials.
authors.affiliation: Division of Molecular and Cellular Biology, School of Veterinary Science, University of Bristol, Langford, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Bishop
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
authors.forename: T J
authors.initials: TJ
authors.lastname: Gruffydd-Jones
authors.forename: C V
authors.initials: CV
authors.lastname: Whiting
authors.forename: J E
authors.initials: JE
authors.lastname: Humphries
authors.forename: R
authors.initials: R
authors.lastname: Osborne
authors.forename: M
authors.initials: M
authors.lastname: Papanastasopoulou
authors.forename: D A
authors.initials: DA
authors.lastname: Harbour
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D005434:Flow Cytometry; D015151:Immunoblotting; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines