title: Transcription regulatory signals in the 5' and 3' regions of Oreochromis aureus ER gene.
abstract: The Oreochromis aureus estrogen receptor (OaER) 5' region is 62.2% AT-rich and does not display well-positioned consensus TATA and CAAT boxes. A functional imperfect 13 bp ERE, TGTTAtggTGACC, deviating at 2 bases is located in the leader exon. Transient transfection assays indicate that this ERE confers a 5-fold increase in SEAP reporter gene activity at 20 h post E2-induction. The continued elevation of transcription after the initial peak could be responsible for the "memory' effect of E2-induced vitellogenesis. The 4.4 kb OaER 3' region is AT-rich and has a high representation of 1/2 EREs and GREs. There are 10 copies of the destabilizing pentamer ATTTA which, in transient transfection experiments strongly suppressed SEAP activity. The 3' EREs are functional, and on their own, induce a bimodal increase in SEAP activity of 9-fold at 6 h and 11-fold at 18 h post-E2 induction. A recombinant construct of SEAP gene flanked by the 5' upstream and 3' flanking regions of the OaER gene allowed these regulatory signals to "cross-talk' to achieve a 5- and 25-fold increase for the first and second peaks, respectively. This bimodal response to E2-induction is attributed to both transcriptional and translational controls over the SEAP gene. This work represents a novel illustration of a synergistic interaction between the 5' and 3' regulatory elements of a steroid receptor in autoregulation.
authors.affiliation: School of Biological Sciences, National University of Singapore, Singapore.
authors.forename: N S
authors.initials: NS
authors.lastname: Tan
authors.forename: T J
authors.initials: TJ
authors.lastname: Lam
authors.forename: J L
authors.initials: JL
authors.lastname: Ding
mesh_terms: D000818:Animals; D005399:Fishes; D005786:Gene Expression Regulation; D010957:Plasmids; D011960:Receptors, Estrogen / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D011960:Receptors, Estrogen