Antigen-specific sulphidoleukotriene production and histamine release in pollinic patients.
We studied sulphidoleukotriene (sLT) production, by means of CAST-ELISA (Bühlmann) in 92 atopic (54 pollinic and 38 non-pollinic) patients, and in 9 control subjects, after antigenic stimulation of peripheral blood leukocytes with 20 ng/ml and 2 ng/ml of Lolium perenne pollen extract, in the presence of IL-3. Antigen-specific stimulation of leukocytes from pollinic patients studied during the pollen season led to a sLT production significantly higher (p = 0.03 at 2 ng allergen/ml) than in those studied out of the pollen season. Histamine release was also significantly higher in pollen season than out of the season (p = 0.04 at 20 ng allergen/ml and p < 0.001 at 2 ng allergen/ml). There was a significant positive correlation between sLT production and histamine release (r = 0.67 at 2 ng allergen/ml and r = 0.57 at 20 ng/ml, both p < 0.001), and between sLT production and skin test results (r = 0.5 at 2 ng allergen/ml and r = 0.46 at 20 ng allergen/ml, both p < 0.001). We found that sLT production was lower, although not significantly, in patients older than 40 years, and histamine release was significantly (p = 0.02) higher in women than in men at 2 ng allergen/ml. We conclude that sLT production in pollinic patients is higher when antigenic pressure is increased in the environment, and that sLT quantification by CAST-ELISA might be useful for evaluation of this sensitization, with analogous results to the histamine release test.
Ferrer
M
M
Department of Allergology and Clinical Immunology, University Clinic Faculty of Medicine, University of Navarra, Pamplona, Spain.
Sanz
M L
ML
Prieto
I
I
Oehling
A
A
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D015289:Leukotrienes / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D008297:Male; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry*
D015289:Leukotrienes; D013438:Sulfhydryl Compounds