Urinary chondroitin sulfates, heparan sulfate and total sulfated glycosaminoglycans in interstitial cystitis.
OBJECTIVE
We compared urinary glycosaminoglycan levels in patients with interstitial cystitis and healthy controls.


METHODS
Total sulfated glycosaminoglycans assayed by dimethylmethylene blue binding and individual glycosaminoglycans analyzed by cellulose acetate electrophoresis were compared in patients with interstitial cystitis and healthy controls. Also, multiple urine samples were obtained from healthy female controls for 2 months to assess the relationship of urinary glycosaminoglycan and creatinine concentrations, and to determine whether glycosaminoglycan excretion changes during the menstrual cycle.


RESULTS
Total sulfated glycosaminoglycan and creatinine concentrations correlated well in random voided samples. Menstrual cycle day did not affect total sulfated glycosaminoglycan levels. Cellulose acetate electrophoresis revealed 3 bands corresponding to chondroitin sulfates, heparan sulfate and acidic glycoprotein. Patients with interstitial cystitis had decreased urinary concentrations of each of these individual components and total sulfated glycosaminoglycans. However, glycosaminoglycan-to-creatinine ratios were similar in interstitial cystitis and control urine.


CONCLUSIONS
Using these assays total and individual urinary glycosaminoglycan levels normalized to creatinine were not altered in interstitial cystitis.
Erickson
D R
DR
Department of Surgery (Division of Urology), Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, USA.
Ordille
S
S
Martin
A
A
Bhavanandan
V P
VP
D002809:Chondroitin Sulfates / Q000652:urine; D018856:Cystitis, Interstitial / Q000652:urine*; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006493:Heparin / Q000652:urine; D006801:Humans; D008597:Menstrual Cycle / Q000652:urine
D006025:Glycosaminoglycans; D006493:Heparin; D002809:Chondroitin Sulfates