title: High-performance liquid chromatographic determination of human serum albumin in plasma and urine by post-column fluorescence enhancement detection using 8-anilino-1-naphthalenesulfonic acid.
abstract: A method for the quantitative determination of human serum albumin in plasma and urine by post-column high-performance liquid chromatography with fluorescence detection is presented. Human serum albumin in plasma and urine is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection uses the fluorescence enhancement observed when 8-anilino-1-naphthalenesulfonic acid binds to human serum albumin. The method is rapid to perform and is highly sensitive (detection limit is 2.5 ng/injection volume at a signal-to-noise ratio of three.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ishida
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000328:Adult; D000368:Aged; D000419:Albuminuria; D000817:Anilino Naphthalenesulfonates; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D000817:Anilino Naphthalenesulfonates; D012709:Serum Albumin; C027132:1-anilino-8-naphthalenesulfonate