title: A kit for the instant preparation of 99Tcm-citrate for tumour and inflammation scintigraphy.
abstract: A lyophilized kit has been developed to produce 99Tcm-citrate (99%) instantly when mixed with 99TcmO4-. The ingredients of the kit, citrate and stannous chloride, are in a ratio of 100:1 (w/w) and are bound to 700 MBq 99Tcm, sufficient for use in a single patient. The shelf life of the kit when kept refrigerated is at least 3 months. The blood clearance and organ distribution of 99Tcm-citrate have been studied in rabbits and mice respectively, showing its fast clearance (55% at 5 min) and low uptake by the reticulo-endothelial system and other organs. The main route of clearance is via the kidneys. Scintigraphic studies in experimental mice have demonstrated high levels of accumulation of 99Tcm-citrate in turpentine-induced abscess (target-to-non-target ratio 4.2:1) and EAT implanted tumour in mice (T/NT 5:1) 1 h after its administration. In keeping with these animal studies, low uptake of 99Tcm-citrate by normal organs and fast blood clearance have been demonstrated in human patients with infection and tumour as early as 5 min post-administration. This study documents a new kit developed for the preparation of 99Tcm-citrate suitable for the scintigraphic assessment of abscess, infection and tumour.
authors.affiliation: Department of Radiopharmaceuticals, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: U P
authors.initials: UP
authors.lastname: Chauhan
authors.forename: P
authors.initials: P
authors.lastname: Mishra
authors.forename: K
authors.initials: K
authors.lastname: Chuttani
authors.forename: A
authors.initials: A
authors.lastname: Bhatnagar
authors.forename: C S
authors.initials: CS
authors.lastname: Bal
mesh_terms: D000038:Abscess / Q000000981:diagnostic imaging / Q000378:metabolism; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging*; D002951:Citrates / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D008657:Metabolic Clearance Rate; D051379:Mice; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D011817:Rabbits; D011877:Radionuclide Imaging; D011933:Reagent Kits, Diagnostic; D014018:Tissue Distribution; D014425:Turpentine
chemical_list: D002951:Citrates; D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; C030490:technetium Tc 99m citrate; D014425:Turpentine