Chromosomal localization of the genes (CLNS1A and CLNS1B) coding for the swelling-dependent chloride channel ICln.
ICln is a cloned chloride channel paramount for regulatory volume decrease. Two different loci that carry the coding region for ICln were identified in the human genome. By PCR strategies an intronless copy of the gene was located on chromosome 6 at position 6p12.1-6q13 (CLNS1B). By fluorescence in situ hybridization a copy carrying introns with a putative length of 19 kb was located at chromosome 11 on position 11q13.5-q14.1 (CLNS1A). The characterization and chromosomal localization of the ICln gene offer the opportunity to study the regulatory sites of this gene in greater detail and could be helpful in establishing linkages between ICln and potential human diseases.
Nagl
U O
UO
Department of Physiology, University of Innsbruck, Innsbruck, A-6020, Austria.
Erdel
M
M
Schmarda
A
A
Seri
M
M
Pinggera
G M
GM
Gschwentner
M
M
Duba
C
C
Galietta
L J
LJ
Deetjen
P
P
Utermann
G
G
Paulmichl
M
M
D018118:Chloride Channels / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007473:Ion Channels; D016133:Polymerase Chain Reaction
C410035:CLNS1A protein, human; D018118:Chloride Channels; D018076:DNA, Complementary; D007473:Ion Channels