[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization].
Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.
Gurskaia
N G
NG
Shagin
D A
DA
Luk'ianov
K A
KA
Vagner
L L
LL
Shtutman
M S
MS
Musatkina
E A
EA
Moinova
E V
EV
Tatosian
A G
AG
Luk'ianov
S A
SA
Sverdlov
E D
ED
D000595:Amino Acid Sequence; D066258:Amphiregulin; D000818:Animals; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012377:Rodentia; D017386:Sequence Homology, Amino Acid
C587848:AREG protein, human; D066258:Amphiregulin; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins
Klonirovanie s pomoshch'iu vychitaiushcheĭ gibridizatsii kDNK gena ha-SDGF iz linii kletok siriĭskogo khomiachka s povyshennym potentsialom metastazirovaniia.