Defined amino acids in the gag proteins of human immunodeficiency virus type 1 are functionally active during virus assembly.
A structurally highly ordered arrangement of the polyprotein precursor, Pr55gag is a necessary prerequisite for assembly, budding and maturation of the human immunodeficiency virus type 1 (HIV-1). In particular, distinct regions of the matrix protein (p17) and the capsid protein (p24) contained within Pr55gag are functionally active during these processes. In order to determine such regions we exchanged amino acid triplets within p17 (amino acids 46-61) and p24 (amino acids 341-352) for alanine residues and deleted the whole regions. Synthetic peptides derived from these regions had been shown previously to inhibit the production of infectious virus. The effect of the mutations on the release of viral particles was investigated by using recombinant baculoviruses for the expression of mutated Pr55gag as virus-like particles and by use of the respective HI proviruses for monitoring the production of infectious particles.
Kattenbeck
B
B
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Deutschland.
Rohrhofer
A
A
Niedrig
M
M
Wolf
H
H
Modrow
S
S
D000596:Amino Acids; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D003673:Defective Viruses; D015683:Gene Products, gag / Q000502:physiology*; D015488:HIV Antigens / Q000502:physiology; D016655:HIV Core Protein p24 / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D011498:Protein Precursors / Q000502:physiology*; D011533:Proviruses; D011993:Recombinant Fusion Proteins / Q000502:physiology; D014764:Viral Proteins; D014771:Virion / Q000502:physiology; D019065:Virus Assembly / Q000502:physiology*; D054301:gag Gene Products, Human Immunodeficiency Virus
D000596:Amino Acids; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1; C059767:p55 gag precursor protein, Human immunodeficiency virus 1