Burn-associated bone disease in sheep: roles of immobilization and endogenous corticosteroids.
To determine the role of immobilization in the pathogenesis of burn-associated bone disease, we selected the sheep as a model to study the effects of burn injury compared with a sham-burned control group. Seven of the sheep were subjected to controlled 40% flame burn, and seven underwent anesthesia with arterial and venous cannulation but without burn. After labeling newly formed bone with tetracycline and calcein, the sheep were killed 2 weeks after burn or sham burn, and the iliac crest and lumbar vertebrae were analyzed for histomorphometry. Analysis failed to demonstrate a significant reduction of bone formation rate in the burned sheep. Osteoid area and surface and osteoblast surface, which correlated significantly with bone formation rate (r = 0.49, p < 0.025), were reduced in the burned sheep. Results suggest that immobilization may play a primary role in the pathogenesis of burn-associated bone disease, but the presence of differences in other histomorphometric features indicates the bone disease is multifactorial.
Klein
G L
GL
Shriners Burns Institute, Galveston, TX, USA.
Kikuchi
Y
Y
Sherrard
D J
DJ
Simmons
D J
DJ
Biondo
N
N
Traber
D L
DL
D000305:Adrenal Cortex Hormones / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D015519:Bone Density; D001847:Bone Diseases / Q000150:complications / Q000473:pathology; D001862:Bone Resorption / Q000209:etiology / Q000473:pathology; D002056:Burns / Q000150:complications* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D012016:Reference Values; D012307:Risk Factors; D012756:Sheep
D000305:Adrenal Cortex Hormones