Functional analysis of the genes of yeast chromosome V by genetic footprinting.
Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.
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Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Medicine, Stanford, CA 94305, USA. pbrown@cmgm.stanford.edu
Chou
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Lashkari
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Botstein
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Brown
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D015825:Chromosomes, Fungal / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D018983:DNA Footprinting; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D016254:Mutagenesis, Insertional; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
D003470:Culture Media; D004251:DNA Transposable Elements; D004271:DNA, Fungal