Nerve growth factor rescues PC12 cells from apoptosis by increasing amount of bcl-2.
Nerve growth factor (NGF) suppressed the decrease in number of viable PC12 cells after serum withdrawal from culture medium. Accordingly, the amount of bcl-2, a suppressive effector of apoptosis, increased in these cells. Bcl-2 antisense oligonucleotide suppressed not only the NGF-induced increase in bcl-2 but also NGF-induced neuronal differentiation. Results of fluorescent DNA staining indicated that NGF inhibited the chromatin condensation of PC12 cells resulting from serum withdrawal and further that the bcl-2 antisense oligonucleotide canceled this effect of NGF. The present results suggest that NGF rescues PC12 cells from apoptosis induced by serum withdrawal via up-regulation of bcl-2.
Katoh
S
S
Radioisotope Research Institute, Faculty of Medicine, University of Tokyo, Japan.
Mitsui
Y
Y
Kitani
K
K
Suzuki
T
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015854:Up-Regulation
D009414:Nerve Growth Factors; D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2