Technetium-99m antibodies labeled with MAG3 and SHNH: an in vitro and animal in vivo comparison.
The in vitro stability and animal pharmacokinetics of 99mTc bound to Sandoz and C110 IgG antibodies via a modified MAG3 has been compared with the hydrazino nicotinamide (SHNH) moiety as standard. For both antibodies, the stabilities of the label to challenge at up to 50:1 cysteine: IgG molar ratio were comparable, but at higher molar ratios, MAG3 showed greater instabilities. For the Sandoz antibody, size-exclusion HPLC analysis of 37 degrees C serum incubates and plasma samples from injected mice showed no clearly distinguishable differences. In the C110 case, some increased high molecular weight radioactivity was apparent with MAG3. Biodistributions in normal mice showed significant differences only in liver (Sandoz) and liver, spleen, intestines, stomach, and blood (C110), with SHNH usually providing higher levels. Thus, for two different antibodies and under the conditions of this study, the MAG3 chelator provided a 99mTc label with properties similar to that of SHNH moiety.
Lei
K
K
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
Rusckowski
M
M
Chang
F
F
Qu
T
T
Mardirossian
G
G
Hnatowich
D J
DJ
D000818:Animals; D003432:Cross-Linking Reagents; D003545:Cysteine; D004355:Drug Stability; D007074:Immunoglobulin G; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009536:Niacinamide / Q000031:analogs & derivatives*; D013388:Succinimides; D013667:Technetium / Q000493:pharmacokinetics*; D017263:Technetium Tc 99m Mertiatide / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D013388:Succinimides; C073102:succinimidyl 6-hydrazinonicotinate; D009536:Niacinamide; D017263:Technetium Tc 99m Mertiatide; D013667:Technetium; D003545:Cysteine