Patterned staining by fluorescein-labeled oligonucleotides obtained by in vitro selection.
We describe a new means of surface staining using a fluorescent oligonucleotide. A folic acid-specific, fluorescent-labeled oligonucleotide was selected from a group of randomly sequenced oligonucleotides by repeated elution through a folic acid-immobilized column and amplified by the polymerase chain reaction. We then examined the oligonucleotide scattered on a polyester film with folic acid immobilized in a defined pattern, by means of laser fluorescence microscopy. The results showed that the selected oligonucleotide fluoresced exactly following the pattern of immobilized folic acid.
Kawazoe
N
N
Department of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
Ito
Y
Y
Imanishi
Y
Y
D019793:Fluorescein; D005452:Fluoresceins / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides; D013194:Staining and Labeling / Q000379:methods*
D005452:Fluoresceins; D009838:Oligodeoxyribonucleotides; D005492:Folic Acid; D019793:Fluorescein