Effect of bone wax extract on the frequency of bone marrow erythrocyte micronuclei in mice.
The present study evaluated the induction of micronuclear potency of indigenously developed bone wax saline extract. The experiment was designed with 2 batches, each consisting of 8 groups with 3 male Swiss albino mice each. In each batch, the first 3 groups received Chitra's bone wax saline extract and the second 3 groups received Johnson & Johnson bone wax saline extract at 12.5, 25.0 or 50.0 ml/kg body weight ip for 2 d. The remaining 2 groups were vehicle (saline) and positive (cyclophosphamide) controls. Animals were sacrificed 24 and 36 h after the second dosing and bone marrow smears were prepared and evaluated for micronuclei. The Chitra's and Johnson & Johnson's bone wax extracts failed to induce micronuclei in bone marrow erythrocytes of mice.
Mohanan
P V
PV
Toxicology Group, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram, India.
Rathinam
K
K
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage / Q000633:toxicity; D014885:Waxes / Q000633:toxicity*
D001672:Biocompatible Materials; D012462:Saline Solution, Hypertonic; D014885:Waxes; D003520:Cyclophosphamide