Isolation and characterization of the Bacillus subtilis tryptophanyl-tRNA synthetase gene (trpS) conferring 5-fluorotryptophan resistance and temperature sensitivity.
The mutant trpS gene of Bacillus subtilis encoding a thermosensitive tryptophanyl-tRNA synthetase that also confers resistance to growth inhibition by 5-fluorotryptophan at permissive temperature has been isolated and characterized. A point mutation at codon 82 of the gene from a wild-type TCA codon for Ser to a TTA codon for Leu has been identified.
Chow
K C
KC
Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, Hong Kong.
Wong
J T
JT
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics; D011487:Protein Conformation; D013696:Temperature; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology; D014369:Tryptophan-tRNA Ligase / Q000737:chemistry / Q000235:genetics*
C026927:5-fluorotryptophan; D014364:Tryptophan; D014369:Tryptophan-tRNA Ligase