[Stabilization of the alpha-fetoprotein structure with sucrose].
By means of scanning microcalorimetry and fluorescent spectroscopy, the addition of sucrose was shown to stabilize the structure of human alpha-fetoprotein (AFP). The stabilizing effect was not eliminated during eight-day dialysis of AFP against a buffer containing no sucrose, but it can be substantially weakened by treating AFP with a specific enzyme, invertase, which splits sucrose into fructose and glucose. This indicates that human AFP is capable of specific sucrose binding.
Kirkitadze
M D
MD
Narizhieva
N V
NV
Tomashevskiĭ
A Iu
AIu
Potekhin
S A
SA
Uverskiĭ
V N
VN
D000244:Adenosine Diphosphate / Q000737:chemistry; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013395:Sucrose / Q000737:chemistry*; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry*
D000509:alpha-Fetoproteins; D013395:Sucrose; D000244:Adenosine Diphosphate; D014508:Urea
Stabilizatsiia struktury alpha-fetoproteina sakharozoĭ.