Reversing effect of dimethyl-4,4'-dimethoxy-5,6,5', 6'-dimethylenedioxybiphenyl-2,2'-dicarboxylate (DDB) on the phenotypes of human hepatocarcinoma cells line.
When human Bel-7402 hepatocarcinoma cell line grew in a medium containing 10(-4) M dimethyl-4,4'-dimethoxy-5,6,5', 6'-dimethylenedioxybiphynyl-2,2'-dicarboxylate (DDB), the secretion of alpha-fetoprotein (AFP) was significantly lower than the control cells, whereas the albumin (ALB) secretion was markedly higher. The activity of gamma-glutamultranspepeidase (gamma-GT) in DDB-treated cells markedly decreased in comparison with the control cells. On the contrary, the activity of tyrosine-alpha-ketoglutarate transaminase (TAT) was higher in DDB-treated cells than in control cells. DDB (10(-4) M) could significantly increase the content of cAMP in Bel-7402 cells, and also enhance the expression of anti-oncogene p53. The results suggest that DDB has reversing effects on the phenotypes of the human Bel-7402 hepatocarcinoma cell line.
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Department of Pharmacology, Chinese Academy of Medical Sciences, Peking Union Medical University, Beijing, PR China. liuxx091@tc.umn.edu
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D001713:Biphenyl Compounds / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D000242:Cyclic AMP / Q000096:biosynthesis; D004149:Dioxoles / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016158:Genes, p53; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D012709:Serum Albumin / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D014444:Tyrosine Transaminase / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D001713:Biphenyl Compounds; D004149:Dioxoles; D009363:Neoplasm Proteins; D012709:Serum Albumin; D016159:Tumor Suppressor Protein p53; D000509:alpha-Fetoproteins; C043146:7-hydroxy-7'-methoxy-4,4'-bis(1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D000242:Cyclic AMP; D005723:gamma-Glutamyltransferase; D014444:Tyrosine Transaminase