The intracellular domain of the second chain of the interferon-gamma receptor is interchangeable between species.
In this report we show that the mouse interferon (IFN)-gamma R1 and IFN-gamma R2 subunits expressed in hamster cells are capable of rendering the cells sensitive to mouse IFN-gamma as measured by induction of class I MHC antigens and the activation of the transcription factor Stat1 alpha. However, these cells showed no antiviral protection in response to IFN-gamma when challenged with vesicular stomatitis virus (VSV) but limited protection when challenged with encephalomyocarditis virus (EMCV). Furthermore, the cytoplasmic domains of the IFN-gamma R2 subunits, like the cytoplasmic domains of the IFN-gamma R1 chains, can be interchanged between species with no loss of biologic activity, demonstrating that the species-specific interaction of the IFN-gamma R1 and IFN-gamma R2 chains involves only the extracellular domains of the two proteins.
Muthukumaran
G
G
Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.
Donnelly
R J
RJ
Ebensperger
C
C
Mariano
T M
TM
Garotta
G
G
Dembic
Z
Z
Poast
J
J
Baron
S
S
Pestka
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D017471:Receptors, Interferon / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D000096743:Interferon gamma Receptor
D000998:Antiviral Agents; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; D017471:Receptors, Interferon; D011993:Recombinant Fusion Proteins; D000096743:Interferon gamma Receptor