Elevated AP-1 DNA-binding activity in rat brain during ethanol withdrawal.
The DNA-binding activity of the transcription factors AP-1, CREB and OCT was investigated in nuclear extracts of brains from rats undergoing ethanol withdrawal. AP-1 DNA-binding activity but not that of CREB or OCT was increased in the cerebral cortex, hippocampus and cerebellum 15 and 17 hr after ethanol withdrawal. A similar increase in AP-1 binding activity was observed in subcortical structures at 17 hr of ethanol withdrawal. No change in any transcription factor DNA-binding activity was observed in the brain stem. Our results suggest that increased expression of immediate early genes is coupled with an increase in DNA-binding function, indicating that they may play a role in the long-term molecular response to ethanol withdrawal.
Beckmann
A M
AM
Department of Biochemistry, University of Queensland, St Lucia, Australia.
Matsumoto
I
I
Wilce
P A
PA
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins; D000431:Ethanol / Q000633:toxicity*; D015870:Gene Expression; D017781:Genes, Immediate-Early; D018398:Homeodomain Proteins / Q000378:metabolism; D051863:Host Cell Factor C1; D008297:Male; D050811:Octamer Transcription Factor-1; D051381:Rats; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494168:Pou2f1 protein, rat; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D000431:Ethanol; D004247:DNA