A specific association of ERK3 with B-Raf in rat hippocampus.
The possible formation of a stable complex between extracellular signal-regulated kinases (ERKs) and Raf protein kinases was investigated in an attempt to understand the molecular mechanism by which Raf protein kinases were differentially regulated in response to the various stimuli, leading to the activation of ERKs in rat hippocampus. ERK3 was found to coelute with B-Raf, but not c-Raf1, after fractionation of rat hippocampal lysates by QMA anionic exchange chromatography. ERK3 was released into soluble supernatant fraction from the B-Raf, but not c-Raf1, immunoprecipitate, after incubation with ATP. These results, taken together, suggest the specific association of ERK3 with B-Raf in rat hippocampus.
Kim
S H
SH
Hansol Biotechnology and Protein Function Research Unit, Korea Research Institute of Bioscience & Biotechnology, KIST, South Korea.
Yang
Y C
YC
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D007150:Immunohistochemistry; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases; D011233:Precipitin Tests; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D051381:Rats; D015398:Signal Transduction
D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases