Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis.
PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90-97% for close linkage) as compared to the standard individual-by-individual analysis.
Ronin
Y I
YI
Institute of Evolution, University of Haifa, Mount Carmel, Israel.
Korol
A B
AB
Fahima
T
T
Kirzhner
V M
VM
Nevo
E
E
D001699:Biometry / Q000379:methods*; D002874:Chromosome Mapping; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D011995:Recombination, Genetic
D005819:Genetic Markers