The influence of interleukin-6 on the growth of human esophageal cancer cell lines.
We reported that human esophageal cancer cell lines (ECC) (YES-1, -2, -3, -4, -5, and -6) produced interleukin-6 (IL-6). We, therefore, investigated the growth effects ([3H]thymidine uptake assay and direct cell count) of IL-6 on these ECC. IL-6 receptor (R) and GP-130 mRNA were detected in all the ECC, using reverse transcriptase-polymerase chain reaction (RT-PCR) assay, and IL-6R was detected in one (YES-3) by immunohistochemical staining. IL-6, anti-IL-6 monoclonal antibody (mAb), or anti-IL-6R mAb caused no reproducible enhancement or suppression of [3H]thymidine uptake by all six ECC. Direct cell count also revealed that the growth enhancement or suppression by IL-6, anti-IL-6 mAb, or anti-IL-6R mAb was relatively small. Particularly, there was no significant sensitivity of YES-3 cells, which definitely produce IL-6 and express IL-6R for IL-6, anti-IL-6 mAb, or anti-IL6R mAb. These results suggest that some esophageal cancers may produce IL-6 and express IL-6R. However, no major interactions between IL-6 and the growth of human esophageal cancer cell lines were detected in this study.
Oka
M
M
Department of Surgery II, Yamaguchi University School of Medicine, Japan.
Iizuka
N
N
Yamamoto
K
K
Gondo
T
T
Abe
T
T
Hazama
S
S
Akitomi
Y
Y
Koishihara
Y
Y
Ohsugi
Y
Y
Ooba
Y
Y
Ishihara
T
T
Suzuki
T
T
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006131:Growth Inhibitors / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000032:analysis / Q000627:therapeutic use*; D018123:Receptors, Interleukin / Q000032:analysis*; D019947:Receptors, Interleukin-6; D018709:Statistics, Nonparametric; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006131:Growth Inhibitors; D015850:Interleukin-6; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D013936:Thymidine