[The physical mapping of human chromosomes. II. The production of unique chromosome-specific DNA fragments using the polymerase chain reaction with oligonucleotide primers to conservative regions of Alu repeats].
Unique DNA fragments localised between Alu-repeats have been produced by PCR. The reaction was carried out with oligonucleotide primers to conservative regions of Alu-repeats. The DNA fragments from different pulls, individual clones, chromosome-specific clonotecs derived from phage lambda, cosmids and individual human chromosomes served as matrixes. The possibilities are discussed of Alu-primer applying in production of exceptional physical features of DNA molecules, suitable for constructing clone couple groups and for direct physical mapping on the DNA of isolated chromosomes, missing the stage of cloning.
Kazakov
V I
VI
Svetlova
M P
MP
Boĭko
V P
VP
Krutilina
R I
RI
Zenin
V V
VV
Aksenov
N D
ND
Shatrova
A N
AN
Bozhkov
V M
VM
Tomilin
N V
NV
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D017931:DNA Primers; D004247:DNA
Fizicheskoe kartirovanie khromosom cheloveka. II. Poluchenie unikal'nykh khromosom-spetsificheskikh fragmentov DNK s pomoshch'iu polimerzanoĭ tsepnoĭ reaktsii s oligonukleotidnymi zatravkami na konservativnye uchastki Alu-povtorov.