Ultrastructural localization of inhibin beta- and somatostatin-28-immunoreactivities in the paraventricular and supraoptic nuclei.
Recent studies have supported the existence of projections to the paraventricular and supraoptic nuclei of the hypothalamus that arise from non-catecholaminergic neurons in the nucleus of the solitary tract, whose terminal distribution is suggestive of interactions with both parvocellular and magnocellular neurosecretory neurons. Pre-embedding immunolabeling methods were used to compare and characterize the termination patterns of axons immunoreactive for two putative markers for this projection system, inhibin beta and somatostatin-28, at the ultrastructural level. Axon terminal profiles stained for either peptide were found to form symmetric or asymmetric junctions predominantly with the shafts of unlabeled dendrites of varying caliber. A small percentage of peptidergic terminals was found in both hypothalamic nuclei to engage in so-called 'shared synapses', where a single terminal profile contacted two postsynaptic elements. Axo-somatic terminations were relatively rarely seen in the supraoptic nucleus, but were somewhat more abundant in the paraventricular nucleus. These comprised principally symmetric junctions onto the somatic membranes of an ostensibly mixed population of cells, some of which bore apparent neurosecretory specializations. Combined immunoperoxidase and immuno-autoradiographic staining methods were used to estimate the extent to which either terminal type interacts with oxytocin neurons. Oxytocin stained elements comprised a minority of the postsynaptic targets of both peptidergic terminal types in the paraventricular nucleus, and a scant majority of those in the supraoptic nucleus. These results support the view that peptidergic neurons in the caudal nucleus of the solitary tract interact synaptically with multiple cell types in the parvocellular division of the paraventricular nucleus, and preferentially with oxytocinergic elements in the
Sawchenko
P E
PE
Laboratory of Neuronal Structure and Function, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Pfeiffer
S W
SW
D000818:Animals; D008297:Male; D008854:Microscopy, Electron; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000378:metabolism*; D029584:Prostatic Secretory Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000378:metabolism*; D053561:Somatostatin-28; D013495:Supraoptic Nucleus / Q000378:metabolism* / Q000648:ultrastructure
D010455:Peptides; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; D013004:Somatostatin; D053561:Somatostatin-28