Comparison of human and Xenopus GATA-2 promoters.
GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5' flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.
Fleenor
D E
DE
Duke University Medical Center, Department of Medicine, Durham, NC 27710, USA.
Langdon
S D
SD
deCastro
C M
CM
Kaufman
R E
RE
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D050989:GATA2 Transcription Factor; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014981:Xenopus; D029867:Xenopus Proteins
D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; C494714:GATA2 protein, Xenopus; C494711:GATA2 protein, human; D014157:Transcription Factors; D029867:Xenopus Proteins; D004247:DNA