title: Heparan sulfate potentiates the autocrine action of basic fibroblast growth factor in astrocytes: an in vivo and in vitro study.
abstract: Increasing evidence indicates that heparan sulfate proteoglycans have a critical role in the regulation of the activity of basic fibroblast growth factor by interacting with it or its receptor. In this study we examined the possibility that heparan sulfate can modulate the basic fibroblast growth factor system at a more fundamental level than activity regulation, by influencing the synthesis of basic fibroblast growth factor and its receptor messenger RNAs. Previous studies in vitro indicate that basic fibroblast growth factor promotes proliferation and differentiation of astrocytes. Accordingly, we examined the possibility that the action of heparan sulfate on the basic fibroblast growth factor system could have a critical role in the modulation of reactivity and/or proliferation of astrocytes in vitro and in vivo. We report that basic fibroblast growth factor applied to pure astrocyte cultures or rat neocortex promoted an increase in the messenger RNA for basic fibroblast growth factor itself and for its receptor. Furthermore, basic fibroblast growth factor applied directly into the brain elicited an increase in messenger RNA for the astrocytic marker glial fibrillary acidic protein. All of these actions, both in vitro and in vivo, were highly potentiated when heparan sulfate was applied in combination with basic fibroblast growth factor. These results suggest that basic fibroblast growth factor regulates astrocytic proliferation or reactivity via an autocrine cascade that involves induction of its own receptor and that this action is modulated by heparan sulfate.
authors.affiliation: Institute for Brain Aging and Dementia, University of California, Irvine 92697-4540, USA.
authors.forename: F
authors.initials: F
authors.lastname: Gómez-Pinilla
authors.forename: S
authors.initials: S
authors.lastname: Miller
authors.forename: J
authors.initials: J
authors.lastname: Choi
authors.forename: C W
authors.initials: CW
authors.lastname: Cotman
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D006497:Heparan Sulfate / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016222:Fibroblast Growth Factor 2; D005904:Glial Fibrillary Acidic Protein; D006497:Heparan Sulfate; D006728:Hormones; D017468:Receptors, Fibroblast Growth Factor