Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
A panel of anti-human antibodies was used in order to investigate their cross-reactivity with canine leukocytes. The labeling was carried out at the microscopic level by immunocytochemical staining. Of 50 antibodies 22 cross-reacted with canine leukocytes from afferent lymph and peripheral blood. All leukocytes reacted with MHM23 (CD18). Two anti-HLA DR antibodies, DK22 and L243, reacted mostly with veiled cells and with PHA-stimulated lymphocytes, whereas TAL1B5 was cross-reactive with all canine lymphocytes. The activation markers CD25, Ki-67, PCNA were identified on PHA-stimulated lymphocytes with ACT1, Ki-67 and PC10 clone produced antibodies. Canine eosinophils reacted with MHM6 (CD23) antibody. A large number of antibodies reacted with canine lymph veiled cells. Canine granulocytes and a subset of lymphocytes were stained with the anti-CD15 antibody only after treatment of cytospins with neuraminidase.
Galkowska
H
H
Department of Surgical Research and Transplantation, Polish Academy of Sciences, Warsaw, Poland.
Waldemar
L O
LO
Wojewodzka
U
U
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004285:Dogs; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D018809:Proliferating Cell Nuclear Antigen / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D007106:Immune Sera; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen