title: [ELISA in the rapid diagnosis of influenza using as the detecting antibodies polyclonal antinucleoprotein sera].
abstract: Hyperimmune polyclonal sera were obtained in order to use them as capture and detecting antibodies in rapid diagnostic of influenza A and B viruses by ELISA. The test shown 100% specificity. The sensitivity in directly detection of influenza viruses in human samples compared of influenza isolations was 49,25% for type A and 53,33% for type B. The sensitivity was increased after 48 hours from inoculation of human samples in cell culture-86,75% for type A detection and 80,0% for type B detection from supernatant mammalian tissue culture (MDCK).
authors.affiliation: Institutul Cantacuzino.
authors.forename: E
authors.initials: E
authors.lastname: Lupulescu
authors.forename: E
authors.initials: E
authors.lastname: Ioniţă
authors.forename: C
authors.initials: C
authors.lastname: Constantinescu
authors.forename: C
authors.initials: C
authors.lastname: Tecu
authors.forename: C
authors.initials: C
authors.lastname: Chiriţă
authors.forename: V
authors.initials: V
authors.lastname: Alexandrescu
mesh_terms: D000914:Antibodies, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007202:Indicators and Reagents; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D009981:Influenza B virus / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D009698:Nucleoproteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D007202:Indicators and Reagents; D009698:Nucleoproteins
vernacular_title: ELISA ĭn diagnosticul rapid al gripei utilizând ca anticorpi detectori seruri policlonale antinucleoproteină.