title: Cloning and sequence analysis of an intron-containing domain from a peptide synthetase-encoding gene of the entomopathogenic fungus Metarhizium anisopliae.
abstract: A gene encoding a putative peptide synthetase has been cloned and partially sequenced from the filamentous fungus, Metarhizium anisopliae. The deduced amino acid sequence of one entire domain and the following spacer is typical of fungal peptide synthetases, showing good conservation of the six expected core sequences. There are two introns within this region, the first interrupting core 5 (RLDLTDIE) of the domain and the second in a conserved area of the spacer region.
authors.affiliation: Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Bailey
authors.forename: M J
authors.initials: MJ
authors.lastname: Kershaw
authors.forename: B A
authors.initials: BA
authors.lastname: Hunt
authors.forename: I C
authors.initials: IC
authors.lastname: Paterson
authors.forename: A K
authors.initials: AK
authors.lastname: Charnley
authors.forename: S E
authors.initials: SE
authors.lastname: Reynolds
authors.forename: J M
authors.initials: JM
authors.lastname: Clarkson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D007438:Introns; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; D010453:Peptide Synthases