Role of adhesion molecules in natural killer cell-induced DNA fragmentation of cytomegalovirus-infected fibroblasts.
We investigated the roles of CD11a, CD11b, CD18, and CD54 adhesion molecules in non-adherent peripheral blood mononuclear cells (NPBMC)-induced DNA fragmentation of cytomegalovirus (CMV)-infected cells. DNA fragmentation in the supernatant from CMV-infected cells and NPBMC was assayed, and cytotoxicity against CMV-infected cells was calculated. Treatment of NPBMC with monoclonal antibodies to CD11a, CD11b, CD18, and CD54 significantly reduced cytotoxicity against CMV-infected cells. A combination of anti-CD11a, anti-CD11b, and anti-CD18 antibodies further inhibited cytotoxicity against CMV-infected cells. Cytotoxicity against CMV-infected cells treated with anti-CD54 antibody was also significantly inhibited. Binding of effector cells to target cells was not affected by treatment of NPBMC or CMV-infected cells with antiadhesion molecule antibodies. These results indicate that LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), and ICAM-1 (CD54) adhesion molecules are involved in natural killer (NK) cell-induced DNA fragmentation in CMV-infected cells.
Ito
M
M
Department of Pediatrics, Mie University School of Medicine, Japan.
Watanabe
M
M
Kamiya
H
H
Sakurai
M
M
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D003601:Cytotoxicity Tests, Immunologic; D053938:DNA Fragmentation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology* / Q000821:virology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1