Separation of chiral polychlorinated biphenyls by micellar electrokinetic chromatography using beta- and gamma-cyclodextrin mixtures in the separation buffer.
Chiral polychlorinated biphenyls (PCBs) 45, 84, 88, 91, 95, 132, 136, 139, 149, 171, 183 and 196 were separated each in its two enantiomers by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Mixtures of beta- and gamma-cyclodextrins were used as chiral modifiers in a 2-(N-cyclohexylamino)ethanesulphonic acid (CHES) buffer containing urea and sodium dodecyl sulphate (SDS) micelles. Separations of multicomponent mixtures of PCBs into their enantiomers were also performed. A mixture of PCBs 45, 88, 91, 95, 136, 139, 149 and 196 was separated into all sixteen enantiomers in an analysis time of approx. 35 min.
Marina
M L
ML
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá de Henares, Madrid, Spain.
Benito
I
I
Díez-Masa
J C
JC
González
M J
MJ
D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D006540:Herbicides / Q000032:analysis*; D010936:Plant Extracts / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines
D006540:Herbicides; D010936:Plant Extracts; D012997:Solvents; D014227:Triazines