Chromosomal assignment of the human gene encoding the Fos-related antigen-2 (FRA2) to chromosome 2p22-p23.
The four members of the Fos gene family give rise to proteins that are part of the AP-1 transcription factor complex. When studying cAMP-induced apoptosis in a leukemia cell line from rat, we found that the Fra-2 gene (coding for the Fos-related antigen-2) became strongly upregulated as the leukemia cells started to die. It was therefore of interest to determine the cytogenetic localization of the human Fra-2 gene (FRA2), including a comparison to chromosomal aberrations observed in leukemia patients. Based on sequence information from the rat and chicken Fra-2 homologs, we were able to PCR-amplify a 4.5-kb genomic fragment covering exon 4 of FRA2. This fragment was employed as probe for both radioactive and fluorescence in situ hybridization to human metaphase chromosomes, allowing us to assign FRA2 to 2p22-p23. The localization of the gene to chromosome 2 was independently verified by PCR amplification of a FRA2-specific fragment from a panel of rodent-human somatic cell hybrids.
Molven
A
A
Center for Molecular Medicine, Haukeland University Hospital, Bergen, Norway. Anders.Molven@gen.haukeland.no
Houge
G
G
Berger
R
R
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D051921:Fos-Related Antigen-2; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; C497872:FOSL2 protein, human; D051921:Fos-Related Antigen-2; D014157:Transcription Factors; D004247:DNA