title: [Is the elimination of osteosarcoma cells with intraoperative "mesh autotransfusion" and leukocyte depletion filters possible?].
abstract: Intraoperative autotransfusion is contraindicated in cancer surgery because of the possible risk of systemic tumor spread. The aim of the present study was to investigate whether a cell saver in combination with a white blood cell depletion filter can remove osteosarcoma cells.
  
  
  METHODS
  A defined number of osteosarcoma cells from an established cell line were added to red cell concentrates and Ringer solution. The tumor cell concentration was 1000/ ml in the first five experiments, 7111/ml in test no. 6, 1667/ml in test no. 7 and 167/ml in test no. 8. Following thorough mixing, each unit was processed separately by a cell saver (DIDECO BT 795/P) in its normal operation mode to produce a red cell concentrate. This red cell concentrate was filtered using a leukocyte depletion filter (PALL BPF 4). Samples were taken before and after processing with the autotransfuser and after filtration with the white cell depletion filter. Cytospin specimens from all samples were examined for osteosarcoma cells by three different methods (Papanicolaou stain, Vimentin antibodies, DNA analysis).
  
  
  RESULTS
  After processing with the autotransfuser, tumor cells were identified in the red cell concentrate. No osteosarcoma cells were evident after the combined use of cell saver and leukocyte depletion filter.
  
  
  CONCLUSIONS
  The sole use of the autotransfuser DIDECO BT 795/P during osteosarcoma surgery is not recommended because of the potential danger of retransfusion of malignant cells. In combination with the leukocyte depletion filter PALL BPF 4, no osteosarcoma cells were identified in the red cell concentrate. Since the adhesiveness of tumor cells from established cell lines may be different from that of tumor cells in the intraoperative salvaged blood, further studies with blood from the surgical field are necessary to determine the efficacy of white cell depletion filters to eliminate osteosarcoma cells.
authors.affiliation: Abteilung für Anaesthesiologie und Operative Intensivmedizin, Klinikum der Justus-Liebig-Universität Giessen.
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: D F
authors.initials: DF
authors.lastname: Kuhn
authors.forename: B
authors.initials: B
authors.lastname: Hinrichs
authors.forename: E
authors.initials: E
authors.lastname: Schindler
authors.forename: T
authors.initials: T
authors.lastname: Dreyer
authors.forename: C
authors.initials: C
authors.lastname: Hirsch
authors.forename: R
authors.initials: R
authors.lastname: Schäffer
authors.forename: G
authors.initials: G
authors.lastname: Hempelmann
mesh_terms: D001804:Blood Transfusion, Autologous / Q000009:adverse effects / Q000295:instrumentation*; D001859:Bone Neoplasms / Q000517:prevention & control* / Q000601:surgery*; D002469:Cell Separation; D004906:Erythrocyte Count; D005374:Filtration; D006801:Humans; D007432:Intraoperative Period; D007962:Leukocytes / Q000502:physiology*; D012516:Osteosarcoma / Q000517:prevention & control* / Q000601:surgery*; D014407:Tumor Cells, Cultured
vernacular_title: Ist die Elimination von Osteosarkomzellen durch "maschinelle Autotransfusion" und Leukozyten-Depletionsfilter möglich?