title: Electron microscopic evaluation of a two-step theory of pore formation by streptolysin O.
abstract: The formation of pores by streptolysin O (SLO) was analyzed in erythrocyte membranes and liposomes by immunoelectron microscopy and electron spectroscopic imaging. The binding of SLO molecules to membranes was temperature independent, while the polymerization of SLO molecules was temperature dependent. Our results also suggest that proteins in erythrocyte membranes are not involved in the formation of SLO rings.
authors.affiliation: Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan. sekiyak@platinum.pharm.kitasato-u.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Sekiya
authors.forename: H
authors.initials: H
authors.lastname: Danbara
authors.forename: K
authors.initials: K
authors.lastname: Yase
authors.forename: Y
authors.initials: Y
authors.lastname: Futaesaku
mesh_terms: D000327:Adsorption; D001426:Bacterial Proteins; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D008081:Liposomes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D013301:Streptolysins / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D008081:Liposomes; D008565:Membrane Proteins; D013301:Streptolysins; C025964:streptolysin O