LambdaZLG6: a phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage.
We describe a vector, lambdaZLG6, combining the high efficiency of cDNA library cloning in bacteriophage lambda with filamentous phage display of cDNA-encoded products. The cDNAs are expressed as fusions to the 3' end of M13 gene VI. The lambdaZLG library is converted to a pZLG6-cDNA phagemid library by in vivo mass excision. Helper phage infection generates a library of phagemid particles displaying the cDNA-encoded products and containing the corresponding nucleotide sequences within.
Jespers
L S
LS
Corvas International N.V., 9000 Gent, Belgium.
De Keyser
A
A
Stanssens
P E
PE
D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D005822:Genetic Vectors
D018076:DNA, Complementary