Alpha 2 beta 1 integrin is required for the collagen and FGF-1 induced cell dispersion in a rat bladder carcinoma cell line.
We have investigated the role of integrins in the epithelial to mesenchymal transition (EMT) induced by either collagen or fibroblast growth factor-1 (FGF-1) in the rat bladder carcinoma cell line NBT-II. The major collagen-binding receptor is the alpha 2 beta 1 integrin. An increase in expression of alpha 2 beta 1 integrin coincided with EMT induced by either collagen or FGF-1. When both inducers were present, a further increase in alpha 2 expression was observed which correlated with an enhancement in the speed of locomotion. Overexpression of human alpha 2 in NBT-II cells did not trigger EMT but rendered cells more sensitive to the dispersing effect of collagen and FGF-1. Anti-human alpha 2 blocking antibodies affected cell scattering and motility induced by either collagen or FGF-1. These data demonstrate that alpha 2 beta 1 integrin is the mediator of the cell scattering effect induced by collagen. They also indicate that a functional alpha 2 integrin is essential for the motile behavior of NBT-II cells during the FGF-1 induced EMT.
Vallés
A M
AM
UMR 144, CNRS, Subcellular Structure and Cellular Dynamics, Institute Curie Research Division, Paris, France.
Boyer
B
B
Tarone
G
G
Thiery
J P
JP
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002277:Carcinoma / Q000473:pathology*; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000502:physiology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D039421:Integrin alpha2; D019012:Integrin beta1 / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism; D051381:Rats; D038981:Receptors, Collagen; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D015703:Antigens, CD; D039421:Integrin alpha2; D019012:Integrin beta1; D016023:Integrins; D038981:Receptors, Collagen; D016220:Fibroblast Growth Factor 1; D003094:Collagen