Expression of human decay accelerating factor (hDAF) in transgenic pigs regulates complement activation during ex vivo liver perfusion--immunopathological findings.
Ex vivo perfusions of human decay accelerating factor-expressing transgenic (n = 3), and nontransgenic (n = 6) porcine livers with human blood revealed a higher degree of organ damage in non-transgenic pig livers. Transgenic livers were protected from immunohistologically detectable complement deposition, despite corresponding IgM and IgG deposits in both groups. Complement activation and consumption of C3 and C4 turned out to be lower in transgenic pig livers. In contrast to livers of normal landrace pigs, livers from genetically manipulated pigs showed no morphological alterations after perfusion.
Pascher
A
A
Institute for Surgical Research, Klinikum Grosshadern, LMU Munich, Germany.
Poehlein
C
C
Storck
M
M
Abendroth
D
D
Mueller-Hoecker
J
J
Koenig
W
W
Young
V K
VK
White
D J
DJ
Hammer
C
C
D000818:Animals; D030801:Animals, Genetically Modified; D018958:CD55 Antigens / Q000235:genetics / Q000502:physiology*; D003167:Complement Activation; D006801:Humans; D008099:Liver / Q000276:immunology / Q000473:pathology*; D010477:Perfusion; D013552:Swine
D018958:CD55 Antigens