Importance of standardization of lipase assays by using appropriate calibrators.
Comparability of lipase catalytic activities was poor when lipase was determined in 50 patients' specimens by a turbidimetric (Boehringer) and a colorimetric (Sigma) assay. Mean values of results differed by a ratio of 2.39. Optimal common conditions were defined for the titration of lipase activity in two commercial calibrators and in a home-purified preparation of human pancreatic lipase (HPL). When using these titers for each calibrator, comparability was greatly improved (ratio = 1.25). This result indicates that a significant part of between-method discrepancy is due to the lack of a reference method for the titration of lipase calibrators. Intermethod behavior of each material was compared with that of patients' specimens. By using HPL as calibrator, comparability was still dramatically improved (ratio = 1.01). This study shows the importance of the validation of a material for defined routine measurement procedures, before its use as calibrator.
Lessinger
J M
JM
Laboratoire de Biochimie Appliquée, UFR des Sciences Pharmaceutiques, Université Louis Pasteur de Strasbourg, Illkirch, France.
Dourson
J L
JL
Férard
G
G
D002118:Calcium / Q000494:pharmacology; D002138:Calibration; D002384:Catalysis; D003089:Colipases / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods / Q000592:standards*; D003840:Deoxycholic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000097:blood* / Q000378:metabolism; D009391:Nephelometry and Turbidimetry / Q000379:methods / Q000592:standards*; D010179:Pancreas / Q000201:enzymology
D003089:Colipases; D003840:Deoxycholic Acid; D008049:Lipase; D002118:Calcium