[ELISA in the rapid diagnosis of influenza using as the detecting antibodies polyclonal antinucleoprotein sera].
Hyperimmune polyclonal sera were obtained in order to use them as capture and detecting antibodies in rapid diagnostic of influenza A and B viruses by ELISA. The test shown 100% specificity. The sensitivity in directly detection of influenza viruses in human samples compared of influenza isolations was 49,25% for type A and 53,33% for type B. The sensitivity was increased after 48 hours from inoculation of human samples in cell culture-86,75% for type A detection and 80,0% for type B detection from supernatant mammalian tissue culture (MDCK).
Lupulescu
E
E
Institutul Cantacuzino.
Ioniţă
E
E
Constantinescu
C
C
Tecu
C
C
Chiriţă
C
C
Alexandrescu
V
V
D000914:Antibodies, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007202:Indicators and Reagents; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D009981:Influenza B virus / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D009698:Nucleoproteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000914:Antibodies, Viral; D007106:Immune Sera; D007202:Indicators and Reagents; D009698:Nucleoproteins
ELISA ĭn diagnosticul rapid al gripei utilizând ca anticorpi detectori seruri policlonale antinucleoproteină.