[Characterization of pancreatic casein plasteins].
Characterization of pancreatic casein plasteins. In the course of the plastein reaction hydrophobic peptides concentrate mainly in the aggregates (plasteins), whilst hydrophilic peptides remain in solution (supernatant). Liquid chromatographic and sequence analytical studies of pancreatic casein plasteins have shown that the aggregates consist mainly of the free amino acids tyrosine, phenylalanine and tryptophan. Plasteins contain, in addition, short-chain peptides, particularly from the C-terminal of beta-casein. Characterization of the functional properties of the plasteins has shown clearly that aggregation of the short-chain peptides and free amino acids is brought by non-covalent, hydrophobic and ionogenic interactions. In the supernatants resulting from the plastein reaction caseinophosphopeptide sequences, in particular from alpha s-casein, were determined.
Chr Lorenzen
P
P
Institut für Chemie und Physik der Bundesanstalt für Milchforschung, Kiel, Deutschland.
Schieber
A
A
Brückner
H
H
Schlimme
E
E
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry* / Q000201:enzymology; D010455:Peptides / Q000032:analysis; D011492:Protein Hydrolysates / Q000032:analysis*
D000596:Amino Acids; D002364:Caseins; D010455:Peptides; D011492:Protein Hydrolysates; C011531:plastein
Charakterisierung pankreatischer Casein-Plasteine.