Interaction of aldehydes with glyoxalase I and the status of several aldehyde metabolizing enzymes of Ehrlich ascites carcinoma cells.
The possible effect of several physiologically important aldehydes has been tested on partially purified glyoxalase I of Ehrlich ascites carcinoma (EAC) cells. The results indicate that D, and L-lactaldehyde are strong non-competitive inhibitors of glyoxalase I and the effect with the D-isomer is more pronounced, whereas both D,L-glyceraldehyde and acetaldehyde are moderately inhibitory and the nature of inhibition is strictly competitive. Moreover, D,L-glyceraldehyde strongly inhibits the utilization of methylglyoxal by intact EAC cells. A search for the presence of several aldehyde metabolizing enzymes in EAC cells indicates that non-specific aldehyde reductase, methylglyoxal reductase, aldehyde dehydrogenase and alcohol dehydrogenase are apparently absent in this rapidly growing, highly de-differentiated malignant cell.
Biswas
S
S
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Calcutta, India.
Bhattacharjee
S
S
Ray
M
M
Ray
S
S
D000079:Acetaldehyde / Q000494:pharmacology*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000449:Aldehyde Reductase / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005985:Glyceraldehyde / Q000494:pharmacology*; D007791:Lactoylglutathione Lyase / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D011765:Pyruvaldehyde / Q000378:metabolism
D000447:Aldehydes; D004791:Enzyme Inhibitors; D005985:Glyceraldehyde; C014634:lactaldehyde; D011765:Pyruvaldehyde; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D000449:Aldehyde Reductase; C031416:D-lactaldehyde dehydrogenase; D000444:Aldehyde Dehydrogenase; D007791:Lactoylglutathione Lyase; D000079:Acetaldehyde