title: Subunit specificity and organ distribution of glutathione transferase-catalysed S-nitrosoglutathione formation from alkyl nitrites in the rat.
abstract: Glutathione transferase (GST)-catalysed S-nitrosoglutathione (GSNO) formation from alkyl nitrites was determined with the homodimers 1-1, 2-2, 3-3, and 4-4 isolated from rat liver. The 4-4 isoform showed a high specificity for the alkyl nitrites. Total GST activities were studied in homogenates from different organs. The liver showed highest GST activity both with amyl nitrite and with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, the activity ratio of amyl nitrite over CDNB being 3.8. In lung and heart, these ratios were 6.2 and 5.7, respectively, indicating a selectivity of these organs for alkyl nitrite metabolism and GSNO formation.
authors.affiliation: Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, F.R.G.
authors.forename: T P
authors.initials: TP
authors.lastname: Akerboom
authors.forename: Y
authors.initials: Y
authors.lastname: Ji
authors.forename: G
authors.initials: G
authors.lastname: Wagner
authors.forename: H
authors.initials: H
authors.lastname: Sies
mesh_terms: D000680:Amyl Nitrite / Q000378:metabolism*; D000818:Animals; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D005982:Glutathione Transferase / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D026422:S-Nitrosoglutathione
chemical_list: D009573:Nitrites; D009603:Nitroso Compounds; D009569:Nitric Oxide; C024225:n-butyl nitrite; D026422:S-Nitrosoglutathione; D000680:Amyl Nitrite; D005982:Glutathione Transferase; D005978:Glutathione