Effects of vanadate and insulin on the activities of selected enzymes of amino acid metabolism in alloxan diabetic rat kidney.
The effects of insulin and the insulin mimetic agent "vanadate" were studied on the activities of alanine aminotransferase, aspartate aminotransferase, glutamate dehydrogenase and arginase in the cytosolic and the mitochondrial fractions of the kidney in control and alloxan induced diabetic rats. An enhancement in the activities of these enzymes were noted in both the fractions of diabetic kidney. Vanadate treatment (0.6 mg/ml in drinking water) of alloxan induced diabetic rats restored the activities of these enzymes almost completely in the cytosolic and partially in the mitochondrial fractions. Vanadate treatment also normalized hyperglycaemia without altering the depressed levels of insulin secretion in diabetic rats. The effect of insulin treatment was found to be the same as that of vanadate in diabetic rats.
Salimuddin
Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Upadhyaya
K C
KC
Baquer
N Z
NZ
D000410:Alanine Transaminase / Q000378:metabolism; D000496:Alloxan; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001119:Arginase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D014638:Vanadates / Q000494:pharmacology*
D000596:Amino Acids; D007328:Insulin; D014638:Vanadates; D000496:Alloxan; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001119:Arginase