title: Alterations in intestinal motility and microflora in experimental acute pancreatitis.
abstract: CONCLUSIONS
  A delay in intestinal transit time appears as an early event in acute pancreatitis, preceding intestinal bacterial overgrowth and translocation.
  
  
  BACKGROUND
  Septic complications, primarily caused by bacteria of enteric origin, are frequent in severe acute pancreatitis. Impairment in intestinal motility probably plays a pathophysiological role in the development of bacterial overgrowth and ensuing translocation.
  
  
  METHODS
  In the present study, the influence of acute pancreatitis on intestinal motility was evaluated by measuring small intestinal transit time in the rat. Acute pancreatitis was induced by the retrograde intraductal infusion of 0.2 mL taurodeoxycholate. Intestinal transit time was studied by intraduodenal injection of Krebs' phosphate-buffered solution labeled with Na2(51)CrCO4, and 1 h small intestinal transit was measured at 1, 3, 12, and 24 h, after induction of pancreatitis. Bacterial overgrowth was evaluated by measuring Escherichia coli counts in the colon and distal small intestine, and bacterial translocation to mesenteric lymph nodes, the liver, spleen, and pancreas was determined.
  
  
  RESULTS
  A delayed small intestinal transit time was noted from 3 h on after induction of acute pancreatitis, with most of the radioactivity retained in the first two intestinal segments. Overgrowth of E. coli was noted 12 h after induction of pancreatitis in both the colon and distal small intestine, and at the same time-point, a significant increase in the incidence of bacterial translocation to mesenteric lymph nodes was seen.
authors.affiliation: Department of Surgery, Lund University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Leveau
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: V
authors.initials: V
authors.lastname: Soltesz
authors.forename: I
authors.initials: I
authors.lastname: Ihse
authors.forename: R
authors.initials: R
authors.lastname: Andersson
mesh_terms: D000208:Acute Disease; D000818:Animals; D018988:Bacterial Translocation; D002756:Cholagogues and Choleretics / Q000494:pharmacology; D003106:Colon / Q000382:microbiology; D005772:Gastrointestinal Transit; D007082:Ileum / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000382:microbiology* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013657:Taurodeoxycholic Acid / Q000494:pharmacology
chemical_list: D002756:Cholagogues and Choleretics; D013657:Taurodeoxycholic Acid