title: Shieh medium supplemented with tobramycin for selective isolation of Flavobacterium columnare (Flexibacter columnaris) from diseased fish.
abstract: Tobramycin was found to be less inhibitory to Flavobacterium columnare (formerly Flexibacter columnaris) than to other fish-associated bacteria. The selective capacity of Shieh medium, an isolation medium described for this species, was markedly enhanced by adding tobramycin at a concentration of 1 microgram/ml.
authors.affiliation: Laboratory of Veterinary Bacteriology and Mycology, University of Ghent, Merelbeke, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Decostere
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: L A
authors.initials: LA
authors.lastname: Devriese
mesh_terms: D000818:Animals; D003470:Culture Media; D005399:Fishes / Q000382:microbiology*; D005417:Flavobacterium / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D014031:Tobramycin
chemical_list: D003470:Culture Media; D014031:Tobramycin