title: [Serologic and molecular HLA typing in patients from Andalucia with Behçet's disease. Genetic and clinical correlations].
abstract: BACKGROUND
  HLA typing was performed in 67 patients with Behçet's disease from Andalucia, Spain to: 1) analyze the association of class I and II molecules with Behçet's disease in Spain; 2) study the clinical correlations and 3) evaluate its diagnostic and/or prognostic role.
  
  
  METHODS
  1) Serologic typing (microlymphocytotoxicity): class I molecules in 67 patients and class II molecules in 47 patients (controls: 223 healthy volunteers). 2) Oligotyping (PCR-SSO): class II molecules (DQB1 and generic DRB1) in 47 patients and 189 controls. 3) STATISTICAL ANALYSIS: chi square test (dicotomic variables) and Student t test (continuous variables), and calculation of the relative risk by the Wolff and Haldane formulas.
  
  
  RESULTS
  The HLA B51 antigen was the most frequently observed in the whole series (p = 0.003) in males with ocular disease (p = 0.0001) and in patients with cutaneous (p = 0.001) and digestive involvement (p = 0.05). The HLA B51-positive males were younger at disease onset (p = 0.01) with neurologic involvement being infrequent (p = 0.03). The HLA B51 antigen was associated with neurologic (p = 0.06) and articular involvement (p = 0.05). The DQB1*0303 was associated with uveitis of bad evolution (p = 0.01). The DR11 and DQB1*0301 were more frequent in HLA B51-positive patients and the DQ5 was negatively associated with Behçet's disease, particularly in the HLA B51-positive patients.
  
  
  CONCLUSIONS
  The study of the HLA antigens provides useful information for the diagnosis of Behçet's disease, aids in differentiating the different clinical forms and has prognostic significance.
authors.affiliation: Unidad de Colagenosis (Servicio de Medicina Interna), Hospital Universitario Virgen del Rocío, Sevilla.
authors.forename: M J
authors.initials: MJ
authors.lastname: Castillo Palma
authors.forename: J
authors.initials: J
authors.lastname: Sánchez Román
authors.forename: C
authors.initials: C
authors.lastname: Ocaña Medina
authors.forename: M F
authors.initials: MF
authors.lastname: González Escribano
authors.forename: A
authors.initials: A
authors.lastname: Núñez Roldán
authors.forename: F
authors.initials: F
authors.lastname: López-Checa
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000235:genetics*; D005260:Female; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D013030:Spain
vernacular_title: Tipificación HLA serológica y molecular en pacientes andaluces con enfermedad de Behçet. Correlaciones geneticoclínicas.