title: Investigation of morphological change of lateral and midline fluid percussion injury in rats, using magnetic resonance imaging.
abstract: OBJECTIVE
  Investigating the time course of morphological changes in experimental traumatic brain injury (TBI) in vivo helps to clarify the mechanism of TBI and develop new therapeutic modalities. We examined the morphological changes in experimental TBI, using magnetic resonance imaging (MRI) in a rat model.
  
  
  METHODS
  We produced lateral fluid percussion injury (LFP) and midline fluid percussion injury (MFP) in rats, using the Yamaki fluid percussion device. The rats were divided into four groups: LFP, MFP, sham LFP, and sham MFP. MRI was performed with a 4.7-T magnetic resonance apparatus 2 days and 90 days after the induction of injury. T1-, T2-, and T2- weighted images were obtained using a surface coil.
  
  
  RESULTS
  Hemorrhage, contusion, and brain edema in LFP models were detected on the 2nd day after injury, and the necrotic tissue was absorbed and replaced by cerebrospinal fluid on the 90th day. In MFP animals, we detected a small hemorrhage in the corpus callosum with minimal brain edema around the hemorrhage on the 2nd day after injury, and on the 90th day, enlarged ventricles and cisterns were observed, indicating brain atrophy.
  
  
  CONCLUSIONS
  MRI, therefore, is useful for plotting morphological changes in experimental TBI in vivo. We report the novel and clinically important finding of brain atrophy after experimental TBI.
authors.affiliation: Department of Neurosurgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: T
authors.initials: T
authors.lastname: Yamaki
authors.forename: N
authors.initials: N
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Umeda
authors.forename: C
authors.initials: C
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: I
authors.initials: I
authors.lastname: Aoki
authors.forename: S
authors.initials: S
authors.lastname: Naruse
authors.forename: S
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000818:Animals; D001284:Atrophy; D001921:Brain / Q000473:pathology; D001924:Brain Concussion / Q000473:pathology*; D001929:Brain Edema / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000473:pathology; D016489:Head Injuries, Closed / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017208:Rats, Wistar