Purification of monospecific polyclonal antibodies from hyperimmune bovine whey using immunoaffinity chromatography.
Whey, a by-product of cheese production, is a potential source of proteins. Immunization of dairy cows in mid-lactation with mouse IgG and dinitrophenyl-keyhole limpet hemocyanin resulted in the formation of antibodies to these antigens in both blood serum and milk. The antibodies remained in whey during cheese making, and were isolated by immunoaffinity chromatography on matrices with immobilized antigens. The isolated monospecific antibodies were pure and retained their reactivity to antigens.
Konecny
P
P
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Brown
R J
RJ
Scouten
W H
WH
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins; D007074:Immunoglobulin G; D051379:Mice; D008894:Milk Proteins / Q000276:immunology*; D000067816:Whey Proteins
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008894:Milk Proteins; D000067816:Whey Proteins; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin