Cloning of the gene encoding honeybee long-wavelength rhodopsin: a new class of insect visual pigments.
Rhodopsins (Rh), G-protein-coupled receptors with seven transmembrane (TM) helices, form the first step in visual transduction in most organisms. Although many long-wavelength (LW) vertebrate opsin sequences are known, less information is available for invertebrate LW sequences. By a combination of RT-PCR and cDNA library screening, we have cloned and sequenced the honeybee LW Rh gene. The deduced protein is composed of 378 amino acids (aa), appears to have seven TM regions, and contains many of the structures and key aa thought to be important for Rh function. Phylogenetic analysis of this sequence in relation to other invertebrate Rh reveals it to be a member of a new group of insect LW Rh.
Chang
B S
BS
Program in Neuroscience, Harvard University, Museum of Comparative Zoology Labs., Cambridge, MA 02138, USA.
Ayers
D
D
Smith
W C
WC
Pierce
N E
NE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D012243:Rhodopsin / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D019476:Insect Proteins; C102003:LW Rh protein, Apinae; D004247:DNA; D012243:Rhodopsin