title: Acetylcholine response in guinea pig outer hair cells. I. Properties of the response.
abstract: The properties of the ACh (acetylcholine) response in guinea pig outer hair cells (OHCs) are not well understood. It has been shown that the response to ACh involves the activation of a Ca2+ dependent K+ selective conductance (referred to as Ksub where sub stands for suberyldicholine). In the present study, we examined the voltage dependence, the time dependence, and the desensitization of the ACh response. In addition, we examined the K+ selectivity of K(sub). These properties are important for aiding in the determination of the type of K+ channels activated by ACh. Patch-clamp technique in the whole-cell mode was used to record from single OHCs isolated from adult pigmented guinea pigs. ACh (100 microM) was applied to the voltage-clamped OHCs and the ACh induced currents (IACh) were measured. A voltage dependence of the ACh response was found with the ACh induced currents decaying monoexponentially at potentials positive to -30 mV. The decay of the ACh induced currents was faster soon after establishing the whole-cell mode of recording when compared to the decay of the currents some time later. This effect, referred to as the time dependence, was different from the desensitization of the response upon prolonged application of ACh. The desensitization of the ACh induced currents was about 50% after 2 min of continuous application of 100 microM ACh. The examined characteristics of the ACh response in guinea pig OHCs indicate a voltage and time dependence of the response and strong K+ selectivity of the Ksub.
authors.affiliation: Kresge Hearing Research Laboratory of the South, Department of Otorhinolaryngology and Biocommunication, Louisiana State University Medical Center, New Orleans 70112-2234, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Nenov
authors.forename: C
authors.initials: C
authors.lastname: Norris
authors.forename: R P
authors.initials: RP
authors.lastname: Bobbin
mesh_terms: D000109:Acetylcholine / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation; D004553:Electric Conductivity; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects*
chemical_list: D015221:Potassium Channels; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium