Anti-endotoxin antibodies directed against Escherichia coli R-1 oligosaccharide core-tetanus toxoid conjugate bind to smooth, live bacteria and smooth lipopolysaccharides and attenuate their tumor necrosis factor stimulating activity.
The reactivity of anti-OS R1-tetanus toxoid serum with various smooth and rough lipopolysaccharides have been determined in enzyme-linked immunosorbent assay (ELISA) and with intact, live smooth bacterial cells in flow cytometry. All tested smooth lipopolysaccharides of R1 core type belonging to nine different O-serotypes reacted strongly with anti-conjugate antibodies. Flow cytometry showed that anti-core antibodies labelled more than 95% of bacterial cells with high intensity of fluorescence. The anti-conjugate serum was able to react with free form of smooth lipopolysaccharides and to inhibit their TNF stimulation activity in in vitro and in vivo models.
Lugowski
C
C
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland. lugowski@immuno.pan.wroc.pl
Niedziela
T
T
Jachymek
W
W
D000818:Animals; D000915:Antibody Affinity; D001681:Biological Assay; D002478:Cells, Cultured; D004731:Endotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D005434:Flow Cytometry; D000922:Immunotoxins / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages; D051379:Mice; D019081:O Antigens / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000378:metabolism*
D004731:Endotoxins; D000922:Immunotoxins; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D013745:Tetanus Toxoid; D014409:Tumor Necrosis Factor-alpha