title: Cloning and sequencing of a cDNA for Akazara scallop troponin T.
abstract: A cDNA clone encoding troponin T of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle has been isolated and sequenced. The complete sequence deduced consists of 314 amino acid residues with a molecular weight of 37,206. Akazara scallop troponin T contains 55 amino acid residues more and 82 residues fewer than rabbit skeletal muscle troponin T and Drosophila melanogaster troponin T, respectively, showing almost the lowest sequence homology with rabbit troponin T (26%) but the highest homology with Drosophila troponin T (33%). Further, high sequence homology was seen in the functional regions: residues 33-120 and 174-227, corresponding respectively to residues 71-158 and 197-250 of rabbit troponin T (tropomyosin-binding regions); and residues 200-204, corresponding to 223 227 of rabbit troponin T (troponin I-binding region). In residues 1-70 (tropomyosin-binding region), however, only six residues are identical with rabbit troponin T.
authors.affiliation: Department of Chemistry, Faculty of Fisheries, Hokkaido University.
authors.forename: A
authors.initials: A
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Ojima
authors.forename: K
authors.initials: K
authors.lastname: Nishita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014336:Troponin / Q000235:genetics*; D020107:Troponin T
chemical_list: D018076:DNA, Complementary; D014336:Troponin; D020107:Troponin T