[Successful treatment of acute non-lymphoblastic leukemia from myelodysplastic syndrome by combination of human macrophage colony-stimulating factor (M-CSF) and low dose cytosine arabinoside: M-CSF-induced proliferation and tyrosine phosphorylation in leukemic blasts].
An 84-year-old woman was admitted with acute non-lymphoblastic leukemia transformed from myelodysplastic syndrome. We examined the signal transduction of the leukemic blasts. Stimulation of the blasts by macrophage colony-stimulating factor (M-CSF) resulted in tyrosine phosphorylation of several cellular proteins. In vitro proliferation of leukemic blasts was stimulated by M-CSF, but not by granulocyte colony-stimulating factor. Based upon these findings, combined therapy with M-CSF and low dose cytosine arabinoside (ara-C) was successful. After her recovery, we confirmed marked reduction of blasts and disappearance of M-CSF-responsive cells. These results suggest that M-CSF could enhance the cytotoxic effect of ara-C on leukemic blasts via its intracellular signaling pathway linked to proliferation.
Hagiwara
S
S
Department of Internal Medicine, Hospital, International Medical Center of Japan.
Yuo
A
A
Nagai
M
M
Takezako
N
N
Soda
Y
Y
Miwa
A
A
Togawa
A
A
Takaku
F
F
D000368:Aged; D000369:Aged, 80 and over; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage*; D001752:Blast Crisis / Q000473:pathology*; D003561:Cytarabine / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016173:Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D003561:Cytarabine; D014443:Tyrosine; D016173:Macrophage Colony-Stimulating Factor