delta opioid receptor expression is induced by concanavalin A in CD4+ T cells.
Expression of delta opioid receptor mRNA in CD4+ T cells purified from Con A-stimulated murine splenocytes was demonstrated by reverse transcription-PCR and sequencing of the PCR products. mu and kappa opioid receptor mRNAs were not detected. Similar results were obtained in a single isolation of CD8+ T cells from Con A-stimulated splenocytes. mRNA for the delta opioid receptor was not detected in CD4+ T cells purified from either freshly isolated splenocytes or splenocytes cultured without Con A, and incubation of purified CD4+ T cells with Con A did not induce delta opioid receptor mRNA. It appears that quiescent CD4+ T cells do not express classic opioid receptors, but the delta-type receptor can be induced upon activation.
Miller
B
B
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA.
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D015870:Gene Expression; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D017465:Receptors, Opioid, delta / Q000502:physiology*; D013154:Spleen / Q000166:cytology
D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D003208:Concanavalin A