Detection of lymphoproliferative responses against pseudorabies virus immediate early protein (IE180) in swine immunized with a modified live virus vaccine.
The immediate early protein (IE180) of pseudorabies virus (PrV) was generated by cycloheximide (CHX) reversal procedures in PrV-infected swine skin cells. Using this IE180 preparation as antigen, specific proliferation was detected in mononuclear cells (PBMNCs) from swine vaccinated with a modified live virus (MLV) PrV vaccine. Two sets of data support this conclusion. (a) PBMNCs of c/cSLA inbred swine vaccinated with an MLV vaccine a year before the test exhibited significant responses against structural virion proteins and IE180. (b) Vaccination of PrV-negative swine with the same MLV vaccine induced a conversion from an unresponsive state against IE180 to one of specific antigen-driven responsiveness. The responses of vaccinated swine against IE180 were significantly higher than their preimmune responses (p < or = 0.003) or to the response of control swine (p < or = 0.045). Moreover, IE180 antigens obtained from lysates of CHX-reversed, PrV-infected cells by heparin/agarose affinity separation also stimulated specific proliferation of PBMNCs from MLV-vaccinated swine, as their proliferative responses were significantly higher than those of unvaccinated swine (p < or = 0.05). These data suggest that PrV IE180 contributes at least in part to the overall cellular response to PrV.
Garmendia
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Department of Pathobiology, University of Connecticut, Storrs 06268, USA.
Tulman
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D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000737:chemistry; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D017874:Immediate-Early Proteins / Q000276:immunology*; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D011446:Prospective Studies; D012189:Retrospective Studies; D013552:Swine; D013556:Swine, Miniature; D014613:Vaccines, Attenuated / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; C047583:pseudorabies virus immediate early proteins; D006493:Heparin; D003513:Cycloheximide