Surface adhesion molecule expression on human blood cells induced by vascular graft materials in vitro.
The expression of surface adhesion molecules on granulocytes, monocytes (CD11a, CD11b, CD11c, CD18, L-selectin), and platelets (P-selectin, gpIIb-IIIa) was determined after incubation with different graft surfaces [expanded polytetrafluoroethylene (ePTFE) or woven Dacron]. Woven Dacron grafts upregulated the CD11b and CD11c surface antigens on both granulocytes and monocytes. Both graft materials demonstrated increased expression of CD11a and CD18 adhesion molecules on white blood cells at 30 min, followed by a downregulation. Maximum L-selectin expression was seen at 120 min on granulocytes and at 90 min on monocytes without differences between the graft materials. A rapid downregulation of gpIIb-IIIa complexes on platelets was noticed, while no expression of platelet P-selectin molecules was observed. In conclusion, both graft materials induced alteration of the white blood cell adhesion molecule expression, but the intensity and time course were dependent on the cell type and the graft material, suggesting that different mechanisms might be implicated. The expression of platelet surface antigens was less clearly influenced. The clinical significance of an enhanced cell surface antigen receptor expression caused by woven Dacron (CD11b, CD11c) has to be further studied. However, determination of adhesion molecule expression might offer possibilities to predict biocompatibility.
Swartbol
P
P
Department of Surgery, Lund University, Sweden.
Truedsson
L
L
Pärsson
H
H
Norgren
L
L
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001807:Blood Vessel Prosthesis; D018821:CD18 Antigens / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis / Q000235:genetics; D019041:L-Selectin / Q000096:biosynthesis / Q000235:genetics; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis / Q000235:genetics; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D019007:P-Selectin / Q000096:biosynthesis / Q000235:genetics; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis / Q000235:genetics; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D011138:Polytetrafluoroethylene / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013696:Temperature
D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011093:Polyethylene Terephthalates; D019041:L-Selectin; D011138:Polytetrafluoroethylene