In-vitro cultivation of exoerythrocytic stages of Plasmodium cynomolgi in hepatocytes of Macaca radiata.
Hepatocytes from bonet monkey (Macaca radiata) obtained by perfusion of a liver biopsy were infected in-vitro with Plasmodium cynomolgi bastianellii sporozoites raised in Anopheles stephensi. The development of exoerythrocytic (EE) stages was seen under phase contrast microscope and by Giemsa staining. Multinucleated EE-stages were seen in the cultured hepatocytes on day 7-8 post-sporozoite inoculation.
Kamboj
K K
KK
Division of Parasitology, Microbiology, Central Drug Research Institute, Lucknow, India.
Puri
S K
SK
Katiyar
J C
JC
Dutta
G P
GP
D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000469:parasitology; D008255:Macaca radiata; D008288:Malaria / Q000469:parasitology*; D008858:Microscopy, Phase-Contrast; D016789:Plasmodium cynomolgi / Q000254:growth & development*; D013170:Spores / Q000302:isolation & purification