Production and purification of Clostridium perfringens alpha-toxin using a protein-hyperproducing strain, Bacillus brevis 47.
Clostridium perfringens alpha-toxin was produced in a protein-hyperproducing strain, Bacillus brevis 47, by cloning the gene into the constructed expression-secretion vector which has the multiple promoters and the signal peptide coding region of an outer cell wall protein gene. The amount of alpha-toxin produced by the B. brevis 47 transformant carrying the gene was approximately 10 times greater than that produced by a B. subtilis transformant carrying the toxin gene. Biological activities and the N-terminal amino acid sequence of the toxin secreted by the B. brevis 47 transformant were identical to those of wild-type alpha-toxin.
Nagahama
M
M
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Michiue
K
K
Sakurai
J
J
D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry* / Q000235:genetics; D001431:Bacteriological Techniques; D001483:Base Sequence; D003016:Clostridium perfringens / Q000737:chemistry* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D014170:Transformation, Genetic; D010738:Type C Phospholipases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
D010738:Type C Phospholipases