title: Crystallization and preliminary X-ray analysis of a thiol-activated cytolysin.
abstract: We present the first reported crystallization of a member of the thiol-activated family of protein toxins. Perfringolysin O, a virulence factor of Clostridium perfringens, has been crystallized in two different forms by the hanging drop vapor diffusion method. In one form the toxin crystallizes with PEG 20000 in the orthorhombic space group C222(1) with cell dimensions of a = 47.8 A, b = 182.0 A and c = 175.5 A and the crystals diffract to beyond 2.5 A resolution. In the second form the toxin crystallizes in a large variety of organic solvents including malt whisky. This crystal form belongs to the orthorhombic space group P222(1) with unit cell dimensions a = 47.1 A, b = 166.1 A and c = 214.0 A and with diffraction observed to 2.4 A resolution.
authors.affiliation: The Ian Potter Foundation Protein Crystallography Laboratory, St. Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
authors.forename: S C
authors.initials: SC
authors.lastname: Feil
authors.forename: J
authors.initials: J
authors.lastname: Rossjohn
authors.forename: K
authors.initials: K
authors.lastname: Rohde
authors.forename: R K
authors.initials: RK
authors.lastname: Tweten
authors.forename: M W
authors.initials: MW
authors.lastname: Parker
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D003016:Clostridium perfringens; D003460:Crystallization; D018360:Crystallography, X-Ray; D006460:Hemolysin Proteins / Q000737:chemistry*; D012997:Solvents; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D012997:Solvents; D013438:Sulfhydryl Compounds; C020452:Clostridium perfringens theta-toxin