RAPD typing in microbiology--a technical review.
Many biochemical and molecular techniques can be used for distinguishing isolates of a given bacterial species. Traditional typing techniques based on phenotypic characteristics such as serotyping are being increasingly challenged by the use of DNA-based methods. The introduction of the polymerase chain reaction (PCR) has led to typing techniques based on DNA amplification. Randomly amplified polymorphic DNA (RAPD) typing (also known as arbitrarily primed-polymerase chain reaction, APPCR) is one such technique which is being used increasingly to type micro-organisms, especially during clinical outbreaks. The application and potential problems and solutions of RAPD typing are discussed and the role of such techniques among established typing methods is addressed.
Power
E G
EG
Department of Microbiology, UMDS, London, UK.
D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000175:diagnosis; D016172:DNA Fingerprinting; D006801:Humans; D008828:Microbiological Techniques; D019105:Random Amplified Polymorphic DNA Technique / Q000592:standards; D015203:Reproducibility of Results