title: LambdaZLG6: a phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage.
abstract: We describe a vector, lambdaZLG6, combining the high efficiency of cDNA library cloning in bacteriophage lambda with filamentous phage display of cDNA-encoded products. The cDNAs are expressed as fusions to the 3' end of M13 gene VI. The lambdaZLG library is converted to a pZLG6-cDNA phagemid library by in vivo mass excision. Helper phage infection generates a library of phagemid particles displaying the cDNA-encoded products and containing the corresponding nucleotide sequences within.
authors.affiliation: Corvas International N.V., 9000 Gent, Belgium.
authors.forename: L S
authors.initials: LS
authors.lastname: Jespers
authors.forename: A
authors.initials: A
authors.lastname: De Keyser
authors.forename: P E
authors.initials: PE
authors.lastname: Stanssens
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D005822:Genetic Vectors
chemical_list: D018076:DNA, Complementary