title: Expression in a wine yeast strain of the Aspergillus niger abfB gene.
abstract: A recombinant wine yeast strain has been constructed expressing the gene coding for alpha-L-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous alpha-L-arabinofuranosidase B shows similar physico-chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The alpha-L-arabinofuranosidase B protein is detected throughout the fermentation.
authors.affiliation: Departamento de Biotecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Sánchez-Torres
authors.forename: L
authors.initials: L
authors.lastname: González-Candelas
authors.forename: D
authors.initials: D
authors.lastname: Ramón
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D005285:Fermentation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D014920:Wine / Q000382:microbiology
chemical_list: D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase