Expression of antimicrobial peptide genes after infection by parasitoid wasps in Drosophila.
We report here the use of a specific beta-galactosidase staining assay and Northern blotting technique to examine the expression of three genes encoding either antibacterial peptides (diptericin, cecropin A) or an antifungal peptide (drosomycin) in Drosophila following infection by larval and pupal parasitoids. The results show that the genes encoding these peptides are either not induced or minimally induced in wasp-infected hosts, but remain responsive and are induced upon microbial challenge. As the parasitoids elicit a cellular response, our data suggest that the antimicrobial responses are activated and/or regulated by mechanisms that are independent of those mediating cellular encapsulation.
Nicolas
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E
Institut de Biologie Moléculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France.
Nappi
A J
AJ
Lemaitre
B
B
D000818:Animals; D030801:Animals, Genetically Modified; D000890:Anti-Infective Agents / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000469:parasitology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D007301:Insect Hormones / Q000235:genetics; D019476:Insect Proteins; D007763:Lac Operon; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis; D014863:Wasps / Q000235:genetics*
D000890:Anti-Infective Agents; C054756:DptA protein, Drosophila; D029721:Drosophila Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D010455:Peptides; D012313:RNA