Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase.
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.
Walfridsson
M
M
Department of Applied Microbiology, Lund Institute of Technology/Lund University, Sweden.
Bao
X
X
Anderlund
M
M
Lilius
G
G
Bülow
L
L
Hahn-Hägerdal
B
B
D019747:Aldose-Ketose Isomerases; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics*; D014994:Xylose / Q000378:metabolism*
D011994:Recombinant Proteins; D000431:Ethanol; D014994:Xylose; D010735:Phosphoglycerate Kinase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase