DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.
A whole array of DNA-fingerprinting techniques, which provide indirect access to DNA sequence polymorphism in order to assess species or clonal identity of bacterial organisms or in order to study bacterial genome composition, have been described during past decades. Nomenclature has been sometimes erroneous and/or confusing, also because of hybrid techniques that combine different approaches. It can be shown that most techniques study the sequence polymorphism of only the chromosome, or only the plasmid(s) or only a gene or gene fragment and that the sequence polymorphism is revealed by AFLP (amplified fragment length polymorphism) or by RFLP (restriction fragment length polymorphism) or by special electrophoresis techniques. Starting from these considerations, some taxonomy of techniques, which enables more appropriate nomenclature, can be developed.
Vaneechoutte
M
M
Department of Clinical Chemistry, Microbiology & Immunology Blok A, University Hospital, Ghent, Belgium. Mario.Vaneechoutte@rug.ac.be
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004274:DNA, Recombinant; D009626:Terminology as Topic
D004274:DNA, Recombinant