Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.
We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
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Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St. Paul 55108, USA.
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Hanson
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Flickinger
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D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D001483:Base Sequence; D015342:DNA Probes; D015280:DNA Restriction-Modification Enzymes / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D015280:DNA Restriction-Modification Enzymes