The effect of a nucleotide-nucleoside solution on hepatic regeneration after partial hepatectomy in rats.
After hepatectomy, purine and pyrimidine metabolism is a key process in the synthesis of DNA and RNA and maintaining cellular energy metabolism. The purpose of this study is to evaluate changes in blood purine and pyrimidine levels after partial hepatectomy and the effect of purine and pyrimidine nucleoside solution injection on hepatic regeneration under the hypothesis that the rat after partial hepatectomy requires substrates for salvage nucleotide synthesis and changes blood nucleoside and nucleobase levels. Blood levels of nucleotides, nucleosides, and nucleobase by high-performance liquid chromatography method and liver ATP level by enzymatic analysis, and the effect of preoperative injection of nucleoside solution (OG-VI) on hepatic regeneration ratio and hepatocytes DNA synthesis, were assessed in rats after 70% partial hepatectomy. Decreased liver adenosine triphosphate and increased plasma xanthine and hypoxanthine after partial hepatectomy indicated an increase in catabolism of purine nucleotides in regenerating liver. Plasma thymidine and cytidine levels increased, then returned to the prevalue, suggesting that the thymidine and cytidine pool was enlarged. OG-VI increased labeling indices of hepatocytes at postoperative d 1 (POD) and hepatic regeneration ratio at POD 14. Blood purine nucleobase and pyrimidine nucleoside levels change after partial hepatectomy and preoperative supply of nucleoside solution is effective for increasing hepatocytes DNA synthesis and hepatic regeneration after partial hepatectomy.
Usami
M
M
First Department of Surgery, Kobe University School of Medicine, Japan.
Furuchi
K
K
Ogino
M
M
Kasahara
H
H
Kanamaru
T
T
Saitoh
Y
Y
Yokoyama
H
H
Kano
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy; D007700:Kinetics; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D009705:Nucleosides / Q000494:pharmacology*; D009711:Nucleotides / Q000494:pharmacology*; D011687:Purines / Q000097:blood / Q000494:pharmacology; D011743:Pyrimidines / Q000097:blood / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D014527:Uric Acid / Q000097:blood
D009705:Nucleosides; D009711:Nucleotides; D011687:Purines; D011743:Pyrimidines; D012996:Solutions; D014527:Uric Acid; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA