Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae.
We have determined the nucleotide sequence of a fragment of chromosome XV of Saccharomyces cerevisiae cloned into cosmid pEOA048. The analysis of the 26,857 bp sequence reveals the presence of 19 open reading frames (ORFs), and of one RNA-coding gene (SNR17A). Six ORFs correspond to previously known genes (MKK1/SSP32, YGE1/GRPE/MGE1, KIN4/KIN31/KIN3, RPL37B, DFR1 and HES1, respectively), all others were discovered in this work. Only five of the new ORFs have significant homologs in public databases, the remaining eight correspond to orphans (two of them are questionable). O5248 is a probable folypolyglutamate synthetase, having two structural homologs already sequenced in the yeast genome. O5273 shows homology with a yeast protein required for vanadate resistance. O5268 shows homology with putative oxidoreductases of different organisms. O5257 shows homology with the SAS2 protein and another hypothetical protein from yeast. The last one, O5245, shows homology with a putative protein of Caenorhabditis elegans of unknown function. The present sequence corresponds to coordinates 772,331 to 799,187 of the entire chromosome XV sequence which can be retrieved by anonymous ftp (ftp. mips. embnet. org).
Boyer
J
J
Unité de Génétique Moléculaire des Levures (URA 1149 du CNRS and UFR927, Université Pierre et Marie Curie), Institut Pasteur, Paris, France.
Michaux
G
G
Fairhead
C
C
Gaillon
L
L
Dujon
B
B
D000373:Agglutinins / Q000235:genetics; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D048369:MAP Kinase Kinase 1; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D018832:Molecular Chaperones; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000235:genetics; D050809:POU Domain Factors; D010453:Peptide Synthases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D014638:Vanadates / Q000494:pharmacology; D000098825:Oxysterol Binding Proteins
D000373:Agglutinins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006023:Glycoproteins; D006360:Heat-Shock Proteins; D048369:MAP Kinase Kinase 1; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D018832:Molecular Chaperones; D010088:Oxidoreductases; D050809:POU Domain Factors; D010453:Peptide Synthases; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014638:Vanadates; C087902:HES1 protein, S cerevisiae; D000098825:Oxysterol Binding Proteins; C086039:MGE1 protein, S cerevisiae; C115278:RPL43a protein, S cerevisiae; C075981:KIN3 protein, S cerevisiae; C023103:folylpolyglutamate synthetase