title: Immunogenicity of HIV-1LAI gp160 and env peptides in squirrel monkey Saimiri sciureus using alumine and experimental adjuvants.
abstract: Since the identification of the HIV virus, important advances have been achieved in the definition of potential subunit vaccines. We investigated the immunogenicity of a recombinant gp160 antigen and of two gp41 peptides from HIV-1LAI associated with seven different adjuvant formulations in squirrel monkeys. All animals were immunized twice with gp160 and then with a gp41 peptide using the same formulation. All adjuvants used led to a subsequent antibody response against gp160, and 55% of the animals immunized developed anti-gp160 antibodies that could neutralize the virus in vitro. Specific anti-gp41 antibody response was also observed. Results obtained underlined the key role of the adjuvant formulation in the antibody response against a given part of the immunogen, and indicate that such immunogenicity-related investigation can be carried out conveniently in the squirrel monkey Saimiri sciureus.
authors.affiliation: Laboratoire d'Immunologie Parasitaire, Institut Pasteur de la Guyane Française, Cayenne, French Guiana, France.
authors.forename: R
authors.initials: R
authors.lastname: Perraut
authors.forename: P
authors.initials: P
authors.lastname: Chouteau
authors.forename: C
authors.initials: C
authors.lastname: Moog
authors.forename: B
authors.initials: B
authors.lastname: Bonnemains
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000534:Alum Compounds / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D015483:HIV Antibodies / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000276:immunology* / Q000494:pharmacology; D015497:HIV-1 / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology* / Q000494:pharmacology; D012453:Saimiri
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D008562:Membrane Glycoproteins; D010455:Peptides; C053512:glycoprotein 41