HLA B44-restricted cytotoxic T lymphocyte responses to the peptides of HCV nucleoprotein residues 81-100 in patients with chronic hepatitis C.
Human leukocyte antigen B44-restricted cytotoxic T lymphocytes (CTLs) recognize an epitope in hepatitis C virus (HCV) nucleoprotein residues 81-100. CTLs that recognize two wild-type peptides 81-100 of HCV genotypes 1b/II and 2a/III were generated from peripheral blood lymphocytes of each of three patients studied. Although CTLs that recognize a wild-type peptide 81-100 of HCV genotypes 1a/I and 2b/IV were not generated from any patient, CTLs that recognize peptide 81-100 of a rare HCV isolate of type 1a/I were generated from two patients. The results suggest that HLA B44-restricted CTLs recognize most, if not all, HCV isolates of types 1b/II and 2a/III and rare variants of type 1a/I and that the wild-type HCV isolates of genotypes 1a/I and 2b/IV may be less immunogenic for HLA B44-restricted CTLs.
Kita
H
H
Third Department of Internal Medicine, University of Tokyo, Japan.
Moriyama
T
T
Kaneko
T
T
Okamoto
H
H
Hiroishi
K
K
Ohnishi
S
S
Imawari
M
M
D000368:Aged; D000956:Antigens, Viral / Q000276:immunology*; D016022:Case-Control Studies; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D005260:Female; D015235:HLA-B Antigens / Q000502:physiology*; D059948:HLA-B44 Antigen; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology*; D006521:Hepatitis, Chronic / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009698:Nucleoproteins / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen; D009698:Nucleoproteins; D014764:Viral Proteins