A recombinant, arginine-glycine-aspartic acid (RGD) motif from foot-and-mouth disease virus binds mammalian cells through vitronectin and, to a lower extent, fibronectin receptors.
The cell-binding abilities of a recombinant, RGD-containing peptide from foot-and-mouth disease virus (FMDV) have been characterized in HeLa and BHK cells. This peptide represents the aa sequence of the solvent-exposed G-H loop of protein VP1 which is involved in cell recognition and infection. The efficiency of the viral motif in promoting cell attachment and spreading is comparable to that shown by fibronectin or vitronectin. Cell binding is inhibited by a monoclonal antibody directed against a viral, RGD-involving B-cell epitope and also by sera against vitronectin (alpha V beta 3/beta 5) and fibronectin (alpha 5 beta 1) receptors. In addition, a synthetic RGD peptide, which is a ligand for both integrins, prevents the cell binding mediated by the FMDV domain. These data demonstrate that the FMDV RGD motif is a potent ligand for cell-receptor integrins and sufficient to promote cell attachment to susceptible cells mainly through the vitronectin receptor.
Villaverde
A
A
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Spain. a.villaverde@cc.uab.es
Feliu
J X
JX
Harbottle
R P
RP
Benito
A
A
Coutelle
C
C
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D005537:Aphthovirus / Q000235:genetics / Q000378:metabolism*; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D017469:Receptors, Fibronectin / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D009842:Oligopeptides; D017469:Receptors, Fibronectin; D011991:Receptors, Virus; D019030:Receptors, Vitronectin; D011993:Recombinant Fusion Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; C047981:arginyl-glycyl-aspartic acid