title: Down-regulation of beta-adrenergic receptor following long-term monocular deprivation in cat visual cortex.
abstract: To examine how adrenergic receptor binding is modified by experimental manipulation of sensory afferent, we carried out binding experiments (membrane fraction and in vitro autoradiography) for both alpha 2- and beta-adrenergic receptors in the brain of cats which had been deprived of vision in one eye. In the cerebral cortex of control animals, beta-adrenergic receptor (beta-AR) binding was found to be higher in the occipital regions than in other regions, while alpha 2-AR binding was relatively uniform. Monocular deprivation throughout the postnatal sensitive period (1-7 month of age) significantly decreased beta-AR binding in the visual cortex and lateral geniculate nucleus. Scatchard plot analysis in the visual cortex showed ca. 50% reduction in Bmax and little change in Kd. No significant difference was found in alpha 2-AR binding following monocular deprivation. Similar extent of down-regulation in beta-AR binding was confirmed in all layers of visual cortex using autoradiography.
authors.affiliation: Department of Neuroscience, Osaka Bioscience Institute, Suita-shi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Muguruma
authors.forename: K
authors.initials: K
authors.lastname: Imamura
authors.forename: H
authors.initials: H
authors.lastname: Morii
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000187:drug effects*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D014793:Visual Cortex / Q000502:physiology*; D015016:Yohimbine / Q000494:pharmacology
chemical_list: D011943:Receptors, Adrenergic, beta; D015016:Yohimbine