A carbon-limited medium for growth and sporulation of Bacillus thuringiensis var. kurstaki.
A culture medium for batch production of d-endotoxin by Bacillus thuringiensis (B., t.) has been modified. Through batch and continuous cultivation studies, the original medium was diagnosed to be limited in organic nitrogen. Corn steep liquor was found to be an excellent source for the organic nitrogen and its addition resulted in a carbon limited medium and in a significant increase in the amount of spore-toxin complex formed in shake flasks. Results of bioassay, conducted on Trichoplusia ni, suggest enhancement of larvicidal efficacy under carbon-limited growth conditions.
Liu
W M
WM
Department of Chemical Engineering, University of Missouri-Columbia 65211, USA.
Bihari
V
V
Bajpai
R
R
D000645:Ammonium Sulfate / Q000737:chemistry; D001413:Bacillus thuringiensis / Q000254:growth & development*; D001427:Bacterial Toxins / Q000378:metabolism; D002021:Buffers; D002122:Calcium Chloride / Q000737:chemistry; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D008278:Magnesium Sulfate / Q000737:chemistry; D017895:Manganese Compounds / Q000737:chemistry; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry
D001427:Bacterial Toxins; D002021:Buffers; D003470:Culture Media; D017895:Manganese Compounds; D010710:Phosphates; D017680:Potassium Compounds; D013431:Sulfates; D002244:Carbon; D008278:Magnesium Sulfate; C013216:potassium phosphate; D002122:Calcium Chloride; D009584:Nitrogen; D000645:Ammonium Sulfate; C039798:manganese sulfate