title: Comparison of human and Xenopus GATA-2 promoters.
abstract: GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5' flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.
authors.affiliation: Duke University Medical Center, Department of Medicine, Durham, NC 27710, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Fleenor
authors.forename: S D
authors.initials: SD
authors.lastname: Langdon
authors.forename: C M
authors.initials: CM
authors.lastname: deCastro
authors.forename: R E
authors.initials: RE
authors.lastname: Kaufman
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D050989:GATA2 Transcription Factor; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; C494714:GATA2 protein, Xenopus; C494711:GATA2 protein, human; D014157:Transcription Factors; D029867:Xenopus Proteins; D004247:DNA