title: Sensitive high-performance liquid chromatographic determination of nifedipine in dog plasma using an automated sample preparation system with laboratory robot.
abstract: Nifedipine, a calcium-channel blocking drug was analysed in dog plasma after oral dosing with two different formulations. Sample preparation was automated with a laboratory robot. Quantitative determination of the drug was performed on a reversed-phase HPLC system with electrochemical detection (ED) using an internal standard. Validation of the analytical method showed that the system is well suited for pharmacokinetic studies on dogs. The assay was linear in the range 1-50 ng/ml. Inter-day and intra-day variability were between 6.43-18.15% C.V. and 1.57-5.53% C.V., respectively.
authors.affiliation: Technical University of Budapest, Institute for General and Analytical Chemistry, Hungary.
authors.forename: V
authors.initials: V
authors.lastname: Horváth
authors.forename: A
authors.initials: A
authors.lastname: Hrabéczy-Páll
authors.forename: Z
authors.initials: Z
authors.lastname: Niegreisz
authors.forename: E
authors.initials: E
authors.lastname: Kocsi
authors.forename: G
authors.initials: G
authors.lastname: Horvai
authors.forename: L
authors.initials: L
authors.lastname: Gödörházy
authors.forename: A
authors.initials: A
authors.lastname: Tolokán
authors.forename: I
authors.initials: I
authors.lastname: Klebovich
authors.forename: K
authors.initials: K
authors.lastname: Balogh-Nemes
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004563:Electrochemistry; D009543:Nifedipine / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D012371:Robotics; D012680:Sensitivity and Specificity; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D002121:Calcium Channel Blockers; D014665:Vasodilator Agents; D009543:Nifedipine