title: In vivo repair of 3'-end deletions in a TCV satellite RNA may involve two abortive synthesis and priming events.
abstract: RNA viruses that do not have the stabilizing features of poly(A) tails or amino acids covalently linked to their 3' ends must develop other means for protecting or repairing their genomes from damage caused by cellular RNases. We previously found that deletions in the single-stranded tails of a satellite RNA (sat-RNA D) associated with turnip crinkle virus are repaired in vivo (C. D. Carpenter and A. E. Simon, 1996, J. Virol. 70, 478-486). We now extend this analysis to show that sat-RNA D transcripts with 3'-end deletions of 5 bases give rise to wild-type sat-RNA, while deletions of 6 to 11 bases result in sat-RNA with additional deletions to the -14 position joined to internal TCV genomic RNA (or other) sequence followed by replacement of the terminal C1-2UGC1-3 motif. In addition, we have determined that the selection of internal TCV sequence used in the repair of sat-RNA D 3' ends is not random and generation of these short TCV segments likely involves primer-mediated synthesis of abortive products facilitated by base-pairing between internal regions of TCV genomic RNA and oligoribonucleotides generated by abortive cycling from the 3' end of the TCV genome.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Carpenter
authors.forename: A E
authors.initials: AE
authors.lastname: Simon
mesh_terms: D019184:Carmovirus / Q000235:genetics*; D017931:DNA Primers; D004260:DNA Repair; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D008957:Models, Genetic; D010942:Plant Viruses / Q000235:genetics*; D019244:RNA, Satellite; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D019244:RNA, Satellite