title: Plasma concentrations of endotoxin and antiendotoxin antibodies in patients with multiple injuries: a prospective clinical study.
abstract: OBJECTIVE
  To investigate the time course of endotoxaemia and its effects on the synthesis of antiendotoxin antibodies in patients with multiple injuries.
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  University hospital, Germany.
  
  
  METHODS
  40 Patients with multiple injuries and 20 healthy volunteers who served as controls.
  
  
  METHODS
  Blood samples were collected up to 10 days after injury and the concentrations of endotoxin, antiendotoxin antibodies to four endotoxins, and for anti-alpha-haemolysin of Staphylococcus aureus were measured. The kinetics of endotoxaemia and antiendotoxin antibodies were investigated.
  
  
  RESULTS
  Endotoxaemia peaked 0-3 hours after injury at 0.425 EU/ml and decreased thereafter to 0.04 EU/ml within five days. Total concentrations of IgM, IgA, and IgG increased continuously after day 3 (p < 0.05), but the specific IgM response to all endotoxins was only temporary and the relative content of specific antibodies to all endotoxins peaked at day 3 (p < 0.05). Antiendotoxin antibodies of IgM class cross-reacted among themselves. There was no general increase in specific antiendotoxin antibodies of IgA and IgG class. The relative content of specific antibodies to alpha-haemolysin of S aureus of all classes of immunoglobulins (IgM, IgA, IgG) remained on the same level from day 0-10.
  
  
  CONCLUSIONS
  Multiple injuries are associated with early and temporary endotoxaemia which causes specific increases in antiendotoxin antibodies of the IgM-class. IgM antibodies to endotoxins cross-react among themselves.
authors.affiliation: Department of Surgery, University of Ulm, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Buttenschoen
authors.forename: D
authors.initials: D
authors.lastname: Berger
authors.forename: N
authors.initials: N
authors.lastname: Hiki
authors.forename: W
authors.initials: W
authors.lastname: Strecker
authors.forename: M
authors.initials: M
authors.lastname: Seidelmann
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D018988:Bacterial Translocation; D003429:Cross Reactions; D004731:Endotoxins / Q000097:blood*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008033:Limulus Test; D008297:Male; D008875:Middle Aged; D009104:Multiple Trauma / Q000097:blood* / Q000276:immunology; D011446:Prospective Studies; D013211:Staphylococcus aureus / Q000276:immunology; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D004731:Endotoxins; D007075:Immunoglobulin M