title: Patterns in donor-specific mRNA and protein production of Th1 and Th2 cytokines by graft-infiltrating lymphocytes and PBMC after heart transplantation.
abstract: We used RT-PCR and ELISA to study the kinetics of IL-2 (Th1) and IL-4 (Th2) both on mRNA and protein level from "naive" PBMC and "primed" graft-infiltrating lymphocytes (GIL) obtained from a heart transplant recipient. For this purpose, these cells were stimulated for 1-48 h with donor and control third-party antigens. Only stimulation of GIL with donor-specific antigen resulted in early detectable IL-2 and IL-4 mRNA and protein levels. Maximal relative IL-2 mRNA levels were significantly higher than maximal relative IL-4 mRNA levels (100-fold) in both GIL and PBMC after donor-specific stimulation. This was accompanied by a maximum protein production of 908 pg/ml IL-2 and 19 pg/ml IL-4 by GIL, and of 82 pg/ml IL-2 and undetectable IL-4 production by PBMC. These results suggest that, after stimulation donor-specific "primed" GIL, and not "naive" PBMC, rapidly produce abundant levels of IL-2 (Th1) and IL-4 (Th2) at both the transcriptional and protein level.
authors.affiliation: University Hospital Rotterdam-Dijkzigt, The Netherlands.
authors.forename: C C
authors.initials: CC
authors.lastname: Baan
authors.forename: N M
authors.initials: NM
authors.lastname: van Besouw
authors.forename: C R
authors.initials: CR
authors.lastname: Daane
authors.forename: A H
authors.initials: AH
authors.lastname: Balk
authors.forename: B
authors.initials: B
authors.lastname: Mochtar
authors.forename: H G
authors.initials: HG
authors.lastname: Niesters
authors.forename: W
authors.initials: W
authors.lastname: Weimar
mesh_terms: D002478:Cells, Cultured; D016027:Heart Transplantation; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D008214:Lymphocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D014019:Tissue Donors
chemical_list: D007376:Interleukin-2; D012333:RNA, Messenger; D015847:Interleukin-4