Autoradiographical and immunohistochemical analysis of receptor localization in the central nervous system.
Quantitative receptor autoradiographic methods have been widely used over the past two decades. Some of the advantages and limitations of these techniques are reviewed here. Comparison with immunohistochemical and in situ hybridization methods is also highlighted, as well as the use of these approaches to study receptor gene over-expression in cell lines. Together, data obtained using these various methodologies can provide unique information on the potential physiological roles of a given receptor protein and/or binding sites in various tissues.
Chabot
J G
JG
Douglas Hospital Research Centre, Department of Psychiatry, McGill University, Quebec, Canada.
Kar
S
S
Quirion
R
R
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002490:Central Nervous System / Q000737:chemistry*; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis; D043643:Receptor, Muscarinic M1; D043587:Receptor, Muscarinic M3; D043588:Receptor, Muscarinic M4; D011956:Receptors, Cell Surface / Q000032:analysis*; D011976:Receptors, Muscarinic / Q000032:analysis; D017476:Receptors, Neuropeptide Y / Q000032:analysis
D012333:RNA, Messenger; D043643:Receptor, Muscarinic M1; D043587:Receptor, Muscarinic M3; D043588:Receptor, Muscarinic M4; D011956:Receptors, Cell Surface; D011976:Receptors, Muscarinic; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor