title: Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis.
abstract: PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90-97% for close linkage) as compared to the standard individual-by-individual analysis.
authors.affiliation: Institute of Evolution, University of Haifa, Mount Carmel, Israel.
authors.forename: Y I
authors.initials: YI
authors.lastname: Ronin
authors.forename: A B
authors.initials: AB
authors.lastname: Korol
authors.forename: T
authors.initials: T
authors.lastname: Fahima
authors.forename: V M
authors.initials: VM
authors.lastname: Kirzhner
authors.forename: E
authors.initials: E
authors.lastname: Nevo
mesh_terms: D001699:Biometry / Q000379:methods*; D002874:Chromosome Mapping; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers