Induction and postranslational expression of G-CSF and RANTES in a first trimester trophoblast cell line by lipopolysaccharide.
OBJECTIVE
Comparatively little is known about the capacity of first trimester trophoblasts to respond to an infection and coordinate an immune response. This study characterizes the LPS induction of G-CSF and RANTES in a first trimester trophoblast cell line.


METHODS
HTR-8/SV neo cells were exposed to LPS (1 micrograms/ml) for 0, 2, 4, 6, 8, and 24 hours in DMEM-Ham's F-12 media supplemented with 10% fetal bovine serum. Cytokine levels in culture supernatants were measured by ELISA. Northern analysis of total RNA was conducted using antisense cytokine probes.


RESULTS
Levels of immunoreactive G-CSF and RANTES from LPS induced cultures at 24 hours were 10-fold and 8.5-fold greater than cytokine levels from non-induced cells at 24 hours, respectively (P < 0.01). Under LPS induction, maximal rates of G-CSF and RANTES transcription occurred at 24 hours and 8 hours, respectively.


CONCLUSIONS
The LPS induction of proinflammatory cytokines in a first trimester trophoblast cell line supports the contention that first trimester trophoblasts participate in cytokine based immune signaling in response to infection.
Svinarich
D M
DM
Department of Obstetrics and Gynecology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
Bitonti
O M
OM
Araneda
H
H
Romero
R
R
Gonik
B
B
D015152:Blotting, Northern; D002460:Cell Line / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D012313:RNA; D014327:Trophoblasts / Q000166:cytology*
D018946:Chemokine CCL5; D008070:Lipopolysaccharides; D016179:Granulocyte Colony-Stimulating Factor; D012313:RNA