title: Structure and organ specificity of an anionic peroxidase from Arabidopsis thaliana cell suspension culture.
abstract: The predominant peroxidase (pI 3.5) (E.C. 1.11.1.7) of an Arabidopsis thaliana cell suspension culture was purified and partially sequenced. Oligonucleotides were designed and a specific probe was obtained. A cDNA clone was isolated from an Arabidopsis cell suspension cDNA library and completely sequenced. The cDNA clone comprised 1194 bp and encodes a 30 residue signal peptide and a 305 residue mature protein (Mr 31,966). The sequence of the mature protein is 95% identical to the well-characterized horseradish peroxidase HRP A2 and has therefore been designated ATP A2. Three introns at positions identical to those found in Arabidopsis and horseradish genes encoding cationic peroxidases were identified. RT-PCR analysis revealed root-specific expression.
authors.affiliation: Department of Protein Chemistry, Institute of Molecular Biology, University of Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Ostergaard
authors.forename: A K
authors.initials: AK
authors.lastname: Abelskov
authors.forename: O
authors.initials: O
authors.lastname: Mattsson
authors.forename: K G
authors.initials: KG
authors.lastname: Welinder
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006031:Glycosylation; D007438:Introns; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018517:Plant Roots / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D009195:Peroxidase