Cross-sensitivity of X-ray-hypersensitive cells derived from LEC strain rats to DNA-damaging agents.
The cross-sensitivity of X-ray-hypersensitive lung fibroblasts from LEC strain (LEC) rats to other DNA-damaging agents was examined. The LEC cells were 2- to 3-fold more sensitive to bleomycin (BLM) that induces DNA double-strand breaks, and to a cross-linking agent, mitomycin C, than the cells from WKAH strain (WKAH) rats, while they were slightly sensitive to alkylating agents, ethyl nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine, but not to UV-irradiation. Although no difference was observed in the initial yields of DNA double-strand breaks induced by BLM between LEC and WKAH cells, the repair process of DNA double-strand breaks was significantly slower in LEC cells than in WKAH cells.
Okui
T
T
Hokkaido Institute of Public Health, Sapporo, Japan.
Endoh
D
D
Arai
S
S
Isogai
E
E
Hayashi
M
M
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003429:Cross Reactions; D004247:DNA / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects*; D006967:Hypersensitivity / Q000235:genetics / Q000662:veterinary*; D008168:Lung / Q000166:cytology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D016685:Mitomycin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014466:Ultraviolet Rays; D014965:X-Rays
D000477:Alkylating Agents; D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D004247:DNA; D005038:Ethylnitrosourea