Construction of a consistent YAC contig for human chromosome region 3p14.1.
Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma. In continuation of our previously published YAC contig of chromosome region 3p14.2-p14.3, we report here on the construction of a YAC contig of at least 11 Mb that consisted of 171 YACs and covers the entire subregion 3p14.1. This contig includes the t(3;8) breakpoint of a hereditary renal-cell carcinoma localized in 3p14.2 and extends into human chromosome region 3p12-p13. It defines the order of 34 DNA probes in relation to reference markers D3S6 and D3S30 as well as the human protein tyrosine phosphatase-gamma gene. For 31 DNA probes we identified nonchimeric YACs by fluorescence in situ hybridization. The minimal tilling pathway consists of 16 yeast artificial chromosomes. As a prerequisite for identification of a putative tumor suppressor gene within this region, this contig renders human chromosome region 3p14.1 accessible to gene isolation.
Bardenheuer
W
W
Innere Klinik und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Germany.
Michaelis
S
S
Lux
A
A
Vieten
L
L
Bröcker
F
F
Jülicher
K
K
Willers
C
C
Siebert
R
R
Smith
D I
DI
van der Hout
A H
AH
Buys
C
C
Schütte
J
J
Opalka
B
B
D015139:Blotting, Southern; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry*; D002893:Chromosomes, Human, Pair 3 / Q000737:chemistry*; D015342:DNA Probes; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D005819:Genetic Markers