title: Construction of a consistent YAC contig for human chromosome region 3p14.1.
abstract: Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma. In continuation of our previously published YAC contig of chromosome region 3p14.2-p14.3, we report here on the construction of a YAC contig of at least 11 Mb that consisted of 171 YACs and covers the entire subregion 3p14.1. This contig includes the t(3;8) breakpoint of a hereditary renal-cell carcinoma localized in 3p14.2 and extends into human chromosome region 3p12-p13. It defines the order of 34 DNA probes in relation to reference markers D3S6 and D3S30 as well as the human protein tyrosine phosphatase-gamma gene. For 31 DNA probes we identified nonchimeric YACs by fluorescence in situ hybridization. The minimal tilling pathway consists of 16 yeast artificial chromosomes. As a prerequisite for identification of a putative tumor suppressor gene within this region, this contig renders human chromosome region 3p14.1 accessible to gene isolation.
authors.affiliation: Innere Klinik und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Bardenheuer
authors.forename: S
authors.initials: S
authors.lastname: Michaelis
authors.forename: A
authors.initials: A
authors.lastname: Lux
authors.forename: L
authors.initials: L
authors.lastname: Vieten
authors.forename: F
authors.initials: F
authors.lastname: Bröcker
authors.forename: K
authors.initials: K
authors.lastname: Jülicher
authors.forename: C
authors.initials: C
authors.lastname: Willers
authors.forename: R
authors.initials: R
authors.lastname: Siebert
authors.forename: D I
authors.initials: DI
authors.lastname: Smith
authors.forename: A H
authors.initials: AH
authors.lastname: van der Hout
authors.forename: C
authors.initials: C
authors.lastname: Buys
authors.forename: J
authors.initials: J
authors.lastname: Schütte
authors.forename: B
authors.initials: B
authors.lastname: Opalka
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry*; D002893:Chromosomes, Human, Pair 3 / Q000737:chemistry*; D015342:DNA Probes; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D015342:DNA Probes; D005819:Genetic Markers