Gastrointestinal absorption of insulinlike growth factor-I in neonatal pigs.
To determine if milk-borne insulinlike growth factor-I (IGF-I) is absorbed in neonatal piglets, recombinant human IGF-I was iodinated and then administered via an oral-gastric tube to newborn and 3-day-old piglets together with fluorescent isothiocyanate dextran (FITC-D70s, a macromolecular marker for assessment of gut closure). Results showed that total radioactivity and trichloroacetic acid (TCA)-precipitable radioactivity rose significantly in the plasma of both newborn and 3-day-old piglets 1 hour after oral-gastric administration of 125I-IGF-I, although the magnitude of increase was greater in newborns than in 3-day-old piglets. In contrast, significant absorption of FITC-D70s was observed only in newborn piglets but not in 3-day-old piglets. Chromatographic analysis revealed that 125I-IGF-I represented up to 20% of total plasma radioactivity in the newborns and up to 10% in the 3-day-old piglets. The 125I-IGF-I found in the plasma was mostly bound to IGF-binding proteins. These results indicate that milk-borne IGF-I can be absorbed in neonatal piglets and the absorption is independent of gut closure.
Xu
R J
RJ
Department of Zoology, University of Hong Kong, Hong Kong.
Wang
T
T
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002850:Chromatography, Gel; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000652:urine; D007408:Intestinal Absorption; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014018:Tissue Distribution
D003911:Dextrans; D018969:Insulin-Like Growth Factor Binding Proteins; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; C015219:fluorescein isothiocyanate dextran; D007334:Insulin-Like Growth Factor I; D016650:Fluorescein-5-isothiocyanate