title: Improved ultrastructural preservation of yeast cells for scanning electron microscopy.
abstract: The processing of yeast cells for scanning electron microscopy by conventional sequential fixation with glutaraldehyde and osmium tetroxide and subsequent dehydration and critical point-drying caused pronounced deformation and visible shrinkage in all basidiomycetous and ascomycetous yeast strains studied. The mean cell diameter decreased to nearly 60 and 70%, respectively. After an additional sequential fixation with 1% tannic acid and 0.5% uranyl acetate the cell shrinkage was significantly reduced, but the most important result was a considerable reduction of wrinkling and deformation of the yeast cells.
authors.affiliation: Institute of Microbiology and Molecular Biology, University of Greifswald, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Hanschke
authors.forename: F
authors.initials: F
authors.lastname: Schauer
mesh_terms: D008855:Microscopy, Electron, Scanning; D016707:Tissue Fixation; D015003:Yeasts / Q000648:ultrastructure*