title: High efficiency photolabeling of human serum albumin and human gamma-globulin with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate.
abstract: The efficiency of photolabeling of HSA and IgG with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate has been studied using size exclusion chromatography in conjunction with liquid scintillation counting. Labeling efficiencies of 78% for HSA and 82% for IgG have been determined. The extent of bond insertion into proteins exceeds the C-H insertion efficiency in cyclohexane with less wastage into anilinium and azo side products. These results suggest that the photoprobe accesses hydrophobic regions of both proteins prior to photolysis.
authors.affiliation: Department of Chemistry, H.S. Truman Memorial VA Hospital, University of Missouri, Columbia 65211, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Pandurangi
authors.forename: S R
authors.initials: SR
authors.lastname: Karra
authors.forename: R R
authors.initials: RR
authors.lastname: Kuntz
authors.forename: W A
authors.initials: WA
authors.lastname: Volkert
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis / Q000737:chemistry*; D001565:Benzoates / Q000138:chemical synthesis / Q000737:chemistry; D002850:Chromatography, Gel; D003510:Cyclohexanes; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D010777:Photochemistry; D010782:Photolysis; D012588:Scintillation Counting; D012709:Serum Albumin / Q000737:chemistry*; D005719:gamma-Globulins / Q000737:chemistry*
chemical_list: D000345:Affinity Labels; D001565:Benzoates; D003510:Cyclohexanes; D007074:Immunoglobulin G; D012709:Serum Albumin; D005719:gamma-Globulins; C098120:methyl 4-azido-2,3,5,6-tetrafluorobenzoate