title: Repression of hybrid dysgenesis in Drosophila melanogaster by heat-shock-inducible sense and antisense P-element constructs.
abstract: Sets of sense and antisense P-element constructs controlled by a heat-shock-inducible promoter were tested for their ability to repress manifestations of P-element activity in vivo. As a group, the antisense constructs repressed pupal lethality, a somatic manifestation of P activity, and this repression was significantly enhanced by heat shock. Three of the 11 antisense constructs also repressed gonadal dysgenesis, a manifestation of P activity in the female germ line; however, none had any effect on P-element-mediated mutability in the male germ line. Among the 13 different heat-shock-inducible sense constructs that were tested, those containing the KP and DP elements were strong repressors of pupal lethality, gonadal dysgenesis and P-element-mediated mutability; however, individual lines carrying these constructs varied in their ability to repress each of these traits, presumably because of genomic position effects. With the exception of the sense construct that contained a complete P element, none of the sense or antisense constructs repressed a lacZ reporter gene driven by the P-element promoter. Overall, the experimental results suggest that in nature, P-element activity could be regulated by P-encoded polypeptides and by antisense P RNAs.
authors.affiliation: Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095, USA. simmo004@maroon.tc.umn.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Simmons
authors.forename: J D
authors.initials: JD
authors.lastname: Raymond
authors.forename: C D
authors.initials: CD
authors.lastname: Grimes
authors.forename: C
authors.initials: C
authors.lastname: Belinco
authors.forename: B C
authors.initials: BC
authors.lastname: Haake
authors.forename: M
authors.initials: M
authors.lastname: Jordan
authors.forename: C
authors.initials: C
authors.lastname: Lund
authors.forename: T A
authors.initials: TA
authors.lastname: Ojala
authors.forename: D
authors.initials: D
authors.lastname: Papermaster
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D018882:Heat Stress Disorders / Q000235:genetics*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D016376:Oligonucleotides, Antisense