title: Recovery of Oesophagostomum dentatum from pigs by isolation of parasites migrating from large intestinal contents embedded in agar-gel.
abstract: Four groups with three pigs in each group were inoculated with Oesophagostomum dentatum larvae (L3 larvae). Groups 1 and 3 were inoculated with 20,000 larvae, and Groups 2 and 4 with 200,000 larvae. On Days 11 and 34, respectively, Groups 1 and 2 and Groups 3 and 4 were slaughtered, and the contents from the large intestines collected. Subsamples of intestinal contents were mixed with agar to a final concentration of 1% agar and allowed to set. The worms were allowed to migrate from the agar-gel into 38 degrees C 0.9% saline overnight. Then the worms were collected on a sieve (38 microns mesh) and counted. The worms retained in the agar-gel were counted after pouring the melted agar through a sieve (38 microns mesh). The results showed that more than 95% of the worms migrated out of the agar-gel, and subsequently were available for counting in an almost clean suspension. Additionally the method yielded a high worm recovery; all stages were recovered. The recovery percentage was not significantly affected by either the dose of parasites or the time interval from slaughtering to start of incubation (37-128 min).
authors.affiliation: Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: H C
authors.initials: HC
authors.lastname: Slotved
authors.forename: E H
authors.initials: EH
authors.lastname: Barnes
authors.forename: H
authors.initials: H
authors.lastname: Bjørn
authors.forename: C M
authors.initials: CM
authors.lastname: Christensen
authors.forename: L
authors.initials: L
authors.lastname: Eriksen
authors.forename: A
authors.initials: A
authors.lastname: Roepstorff
authors.forename: P
authors.initials: P
authors.lastname: Nansen
mesh_terms: D000362:Agar; D000818:Animals; D005260:Female; D005782:Gels; D007420:Intestine, Large / Q000469:parasitology; D007814:Larva; D008297:Male; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary*; D009815:Oesophagostomum / Q000302:isolation & purification*; D010274:Parasitology / Q000379:methods; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D013997:Time Factors
chemical_list: D005782:Gels; D000362:Agar