Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant beta-glucosidase from Streptomyces sp. (ATCC 11238).
The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% alpha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non-ordered structures).
Perez-Pons
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JA
Institut de Biologica Fonamental, Universitat Autònoma de Barcelona, Spain.
Padros
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Querol
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D000465:Algorithms; D000595:Amino Acid Sequence; D001665:Binding Sites; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012984:Software; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry*
D011994:Recombinant Proteins; D001617:beta-Glucosidase