Determination of 8-hydroxydeoxyguanosine in biological tissue by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry.
A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (80HdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific. The method was evaluated by determining increased frequency of formation of 80HdGuo in male Sprague-Dawley rats given a single intraperitoneal dose of Adriamycin compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 10(5) deoxyguanosines was readily achieved.
Serrano
J
J
Midcontinent Ecology Division, U.S. EPA, Duluth, MN 55804, USA.
Palmeira
C M
CM
Wallace
K B
KB
Kuehl
D W
DW
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002853:Chromatography, Liquid; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004317:Doxorubicin / Q000494:pharmacology; D006868:Hydrolysis; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D018384:Oxidative Stress; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley
D000903:Antibiotics, Antineoplastic; D007202:Indicators and Reagents; D004317:Doxorubicin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine