Rapid identification and cloning of bacterial transferrin and lactoferrin receptor protein genes.
The sequences of genes encoding the transferrin and lactoferrin receptor proteins from several bacterial species were analyzed for areas of identity in the predicted protein sequences. Degenerate oligonucleotide primers were designed and tested for their ability to amplify portions of the receptor genes. Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all species possessing these receptors were identified.
Ogunnariwo
J A
JA
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
Schryvers
A B
AB
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D006193:Haemophilus influenzae / Q000235:genetics; D016978:Mannheimia haemolytica / Q000235:genetics; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009345:Neisseria meningitidis / Q000235:genetics*; D015661:Pasteurellaceae / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D011990:Receptors, Transferrin / Q000235:genetics*
D001426:Bacterial Proteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; C038340:lactoferrin receptors