title: Hypothermia suppresses nitric oxide elevation during reperfusion after focal cerebral ischemia in rats.
abstract: We aimed to investigate effect of temperature on the jugular levels of nitric oxide (NO) at reperfusion after focal cerebral ischemia. Both nitrosyl hemoglobin (HbNO) (2.5 +/- 0.4 microM) and plasma nitrite plus nitrate levels (61 +/- 5 microM) in rats under normothermia (approximately 37 degrees C) after 30 min of reperfusion following 2 h of left middle cerebral artery occlusion were significantly high, compared with sham operated rats (1.3 +/- 0.1 microM, 40 +/- 4 microM, respectively). Both HbNO (1.5 +/- 0.3 microM) and nitrite plus nitrate levels (43 +/- 7 microM) under moderate hypothermia (approximately 32 degrees C) were significantly low, compared with normothermic rats. HbNO (2.8 +/- 0.8 microM) and nitrite plus nitrate levels (65 +/- 8 microM) under mild hyperthermia (approximately 39 degrees C) were not significantly high. These results firstly demonstrated that hypothermia suppresses the elevation in intrajugular NO after cerebral ischemia-reperfusion.
authors.affiliation: First Department of Physiology, Osaka University Medical School, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kumura
authors.forename: T
authors.initials: T
authors.lastname: Yoshimine
authors.forename: M
authors.initials: M
authors.lastname: Takaoka
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Shiga
authors.forename: H
authors.initials: H
authors.lastname: Kosaka
mesh_terms: D000818:Animals; D001831:Body Temperature; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy; D006454:Hemoglobins / Q000378:metabolism; D007036:Hypothermia, Induced; D007601:Jugular Veins / Q000378:metabolism; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000097:blood; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D009566:Nitrates; D009573:Nitrites; C008657:nitrosyl hemoglobin; D009569:Nitric Oxide