Inhibition of HIV replication by immunoliposomal antisense oligonucleotide.
The sequence-specific suppression of HIV-1 replication using CD4 monoclonal-antibody-targeted liposomes, containing Rev antisense phosphorothioate oligonucleotides is described. Liposomes were prepared by encapsulating the 20-mer antisense DNA sequence of the rev HIV-1 regulatory gene, in the form of a phosphorothioate oligonucleotide. Specific targeting was accomplished by conjugating anti-CD4 mouse monoclonal antibody to the surface of the liposomes. HIV-1-infected H9 cells as well as peripheral blood T-lymphocytes were incubated with the immunoliposomes of antisense found to have potential antiviral effect. HIV-1 replication was reduced by 85% in antisense immunoliposome-treated H9 cells and peripheral blood lymphocytes, whereas the inhibition of HIV-1 replication was not observed using either empty immunoliposomes or immunoliposomes containing scrambled Rev phosphorothioate oligonucleotide sequences. The antiviral activity of both the free and the encapsulated oligonucleotides were assessed by p24, reverse transcriptase (RT) assays and polymerase chain reaction (PCR) analysis. Liposome preparations demonstrated minimal toxicity in H9 as well as in peripheral blood lymphocyte cell culture experiments. These in vitro culture results demonstrate the potential efficacy of immunoliposomes to inhibit HIV replication.
Selvam
M P
MP
Center for Biologics, Evaluation and Research, US Food and Drug Administration, Rockville, MD 20852, USA. Selvam@al.cher.fda.gov
Buck
S M
SM
Blay
R A
RA
Mayner
R E
RE
Mied
P A
PA
Epstein
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015704:CD4 Antigens / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D015757:Genes, rev; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008081:Liposomes; D051379:Mice; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D004337:Drug Carriers; D008081:Liposomes; D016376:Oligonucleotides, Antisense