Treatment of cat allergy with T-cell reactive peptides.
We induced in allergic humans the counterpart of murine experimental T-cell tolerance. T-cell lines from cat-allergic humans were used to map T-cell epitopes for the principal allergen of cat dander, Fel d 1. Two peptides of 27 amino acids each were synthesized to contain the dominant epitopes (ALLERVAX CAT). After a safety trial, we carried out a blinded study of the dose required for efficacy. We randomly divided 95 cat-sensitive patients into placebo, 7.5 micrograms, 75 micrograms, and 750 micrograms groups. Patients received a subcutaneous injection weekly for 4 wk. Before and after treatment, patients were exposed in a room inhabited by live cats and scored by nose and lung symptoms. Baseline nasal and lung scores (+/-SEM) were 6.2 +/- 0.56 and 5.4 +/- 0.73 in the 750 micrograms group; 7.8 +/- 0.53 and 4.7 +/- 0.68 in the placebo group. Six weeks after treatment, scores adjusted for baseline differences were reduced in the 750 micrograms group: -2.3 +/- 4.9 and -2.3 +/- 0.59 compared with -0.84 +/- 0.50 and -0.85 +/- 0.62 in the placebo group. The 75 micrograms group showed intermediate effects and the 7.5 micrograms group no effect. Linear trend analysis indicated a significant dose response effect: p = 0.05 for nose and 0.03 for lung symptoms. Allergic side effects occurred an hour or more after the first 750 micrograms dose in 16 of 24 patients but required little or no treatment with one exception. T-cell reactive treatment peptides safely improved allergic responses to cats.
Norman
P S
PS
Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224-6801, USA.
Ohman
J L
JL
Long
A A
AA
Creticos
P S
PS
Gefter
M A
MA
Shaked
Z
Z
Wood
R A
RA
Eggleston
P A
PA
Hafner
K B
KB
Rao
P
P
Lichtenstein
L M
LM
Jones
N H
NH
Nicodemus
C F
CF
D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D003888:Desensitization, Immunologic; D004311:Double-Blind Method; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008969:Molecular Sequence Data; D012130:Respiratory Hypersensitivity / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology*
D000485:Allergens; D000939:Epitopes; D006023:Glycoproteins; D007074:Immunoglobulin G; D007073:Immunoglobulin E; C059296:Fel d 1 protein, Felis domesticus