Cerebral oxygenation measured by near infrared spectroscopy during cardiopulmonary bypass and deep hypothermic circulatory arrest in piglets.
Near infrared spectroscopy (NIRS) shows large changes in cerebral oxyhemoglobin (Hbo2), deoxyhemoglobin (Hb), and oxidation state of cytochrome aa3 (Cyto2) in infants undergoing cardiopulmonary bypass and deep hypothermic circulatory arrest (CPB-DHCA). To evaluate the physiologic significance of these clinical NIRS measurements, we applied the technique in a piglet model of CPB-DHCA. After an initial stabilization period on CPB, animals (n = 8) were cooled to 15 degrees C, subjected to DHCA for 1 h, then reperfused with rewarming and monitored for 180 min. NIRS measurements were compared with determinations of cerebral blood flow (CBF). During cooling, Cyto2 decreased markedly, whereas Hbo2 increased. DHCA was associated with a sharp decrease in Hbo2, a corresponding increase in Hb, and a smaller, less consistent further decrease in Cyto2. NIRS measurements recovered toward baseline with reperfusion. CBF decreased during cooling and recovered to baseline levels with reperfusion. These findings are consistent with existing human data and show that 1) cooling is associated with increased oxygenation of cerebral hemoglobin despite a reduction in CBF; 2) Cyto2 becomes more reduced during cooling, consistent with a net cellular oxygen deficit; and 3) DHCA is associated with rapid cerebral hemoglobin deoxygenation and a small further reduction of Cyto2.
Nomura
F
F
Department of Cardiovascular Surgery, Children's Hospital, Boston, Massachusetts 02115, USA.
Naruse
H
H
duPlessis
A
A
Hiramatsu
T
T
Forbess
J
J
Holtzman
D
D
Volpe
J J
JJ
Jonas
R
R
Tsuji
M
M
D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism*; D002315:Cardiopulmonary Bypass; D002560:Cerebrovascular Circulation; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006323:Heart Arrest / Q000503:physiopathology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007036:Hypothermia, Induced; D007223:Infant; D010108:Oxyhemoglobins / Q000378:metabolism*; D015424:Reperfusion; D013055:Spectrophotometry, Infrared / Q000379:methods; D013552:Swine; D013556:Swine, Miniature
D006454:Hemoglobins; D010108:Oxyhemoglobins; D003576:Electron Transport Complex IV