A new adherent form of an attaching and effacing Escherichia coli (eaeA+, bfp-) to the intestinal epithelial cells of chicks.
The adherent site of "attaching and effacing Escherichia coli" (AEEC; O103: H-, SK-1 strain) on the intestinal epithelial cells of chicks infected naturally and experimentally was ultrastructurally investigated. The eaeA gene was detected by polymerase chain reaction in the SK-1 strain of E. coli isolated from the intestinal content of a chick infected naturally, however, the bundle-forming pilus (bfp) gene could not be detected. The SK-1 strain (bfp-) of AEEC could attach to the intestinal epithelial cell and induce attaching-effacing lesions in the intestine of chicks. Transmission electron microscopy revealed numerous pilus-like microfilaments in the space between colibacilli and the membranes of the intestinal epithelial cells. The present study suggests that SK-1 strain (eaeA+, bfp-) may attach closely to the intestinal epithelial cells by a novel adhesion different from bfp.
Sueyoshi
M
M
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
Fukui
H
H
Tanaka
S
S
Nakazawa
M
M
Ito
K
K
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001483:Base Sequence; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002645:Chickens / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004848:Epithelium / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000209:etiology / Q000473:pathology / Q000662:veterinary; D010861:Fimbriae, Bacterial / Q000502:physiology / Q000648:ultrastructure; D005814:Genes, Viral / Q000235:genetics; D007422:Intestines / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000209:etiology / Q000473:pathology
D004279:DNA, Viral