title: Ontogeny of estrogen receptor messenger ribonucleic acid expression in the postnatal rat uterus.
abstract: During the first 2 wk of postnatal life, the rodent uterus undergoes a period of marked growth and differentiation. To further examine the role of the estrogen receptor (ER) in the mediation of uterine development, we analyzed the ontogeny of ER mRNA expression in the postnatal rat uterus using in situ hybridization. ER mRNA was present in the uterine stroma on the day of birth and progressively increased in abundance during the first 2 wk of postnatal life. In contrast, ER mRNA was not detectable in the luminal epithelium at birth and did not become abundant in this region until postnatal day (P) 7. ER mRNA abundance increased in the luminal epithelium and in the invaginating and fully formed glandular epithelium during the second week of life. At P21 ER mRNA was more abundant in the glandular epithelium than in any other uterine cell type. These results are consistent with, and extend the findings of, previous studies using uterine homogenate binding assays and immunohistochemistry to define ER ontogeny in rodents. Delineation of the temporal and cell-type specific pattern of ER mRNA ontogeny in the postnatal rat uterus furthers our understanding of the molecular basis of both endogenous and exogenous estrogen effects on uterine growth and development.
authors.affiliation: National Center for Toxicological Research, U.S. Food and Drug Administration, Department of Health and Human Services, Jefferson, Arkansas 72079, USA. rfishman@nctr.fda.gov
authors.forename: R B
authors.initials: RB
authors.lastname: Fishman
authors.forename: W S
authors.initials: WS
authors.lastname: Branham
authors.forename: R D
authors.initials: RD
authors.lastname: Streck
authors.forename: D M
authors.initials: DM
authors.lastname: Sheehan
mesh_terms: D000375:Aging; D000818:Animals; D001345:Autoradiography; D004848:Epithelium / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics*; D014599:Uterus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen