title: The placement of a striatal ibotenic acid lesion affects skilled forelimb use and the direction of drug-induced rotation.
abstract: The motor consequences of excitotoxic striatal damage have been evaluated extensively in the rat, using tests of whole body motor asymmetry and of deficits in skilled paw and limb movements. However conflicting results of both the type and extent of behavioural deficits have been reported, particularly in the direction of rotation observed in response to the dopamine receptor agonist, apomorphine. The present study investigated the effect of unilateral ibotenic acid lesions in the dorsal striatum of the adult rat, placed at either anterior, posterior, medial, or lateral loci, on rotation in response to both amphetamine and apomorphine, and in the "staircase test" of skilled forelimb use. In a 2 x 2 matrix design experiment, adult female albino rats received a double unilateral lesion of 0.5 microliter 0.06 M ibotenic acid injected at each of two sites either anterior (medial and lateral), posterior (medial and lateral), medial (anterior and posterior), or lateral (anterior and posterior). Rats that received posterior lesions showed a marked ipsilateral rotation in response to both amphetamine and apomorphine, while animals receiving anterior lesions showed little ipsilateral or a slight contralateral bias. Rats receiving lateral lesions showed a marked impairment of contralateral paw use on the "staircase test," while animals with medial lesions showed no significant difference to control unoperated animals. These results confirm the somatotopic organisation of the dorsal striatum in its control of motor functions, and indicate the need to take into account the locus of an excitotoxic lesion in the design of lesion and transplantation studies if we are to achieve reliable tests of the behavioural deficits and recovery.
authors.affiliation: Department of Experimental Psychology, University of Cambridge, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Fricker
authors.forename: L E
authors.initials: LE
authors.lastname: Annett
authors.forename: E M
authors.initials: EM
authors.lastname: Torres
authors.forename: S B
authors.initials: SB
authors.lastname: Dunnett
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D003342:Corpus Striatum / Q000502:physiology*; D005260:Female; D005552:Forelimb / Q000502:physiology*; D007051:Ibotenic Acid / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects*; D051381:Rats; D012399:Rotation
chemical_list: D007051:Ibotenic Acid; D001058:Apomorphine