title: ADP-ribosylation of actin by Clostridium perfringens iota toxin and turkey erythrocyte ADP-ribosyltransferase A: effects on profilin-regulated nucleotide exchange and ATPase activity.
abstract: Effects of ADP-ribosylation of skeletal muscle alpha-actin by Clostridium perfringens iota toxin and by turkey erythrocyte ADP-ribosyltransferase A on profilin-regulated nucleotide exchange and ATPase activity were compared. ADP-ribosylation of actin at Arg 177 by Clostridium perfringens iota toxin increased the nucleotide dissociation rate from 2.2 x 10(-3) s-1 to 4.5 x 10(-3) s-1 without affecting the profilin-induced stimulation of nucleotide exchange. In contrast, ADP-ribosylation of actin at Arg95/Arg372 induced by turkey erythrocyte transferase decreased the nucleotide dissociation rate to 1.5 x 10(3) s-1 and inhibited the profilin-induced stimulation of nucleotide exchange. Whereas toxin-induced ADP-ribosylation at Arg177 blocked actin ATPase, basal G-actin ATPase was not altered by ADP-ribosylation at Arg95/Arg372 but inhibited profilin effects on actin ATPase.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Albert-Ludwigs-Universität Freiburg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Sehr
authors.forename: I
authors.initials: I
authors.lastname: Just
authors.forename: K
authors.initials: K
authors.lastname: Aktories
mesh_terms: D036002:ADP Ribose Transferases; D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002417:Cattle; D003016:Clostridium perfringens / Q000472:pathogenicity*; D003285:Contractile Proteins; D004912:Erythrocytes / Q000201:enzymology*; D008840:Microfilament Proteins / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology*; D051304:Profilins; D011817:Rabbits; D014422:Turkeys
chemical_list: D000199:Actins; D001427:Bacterial Toxins; D003285:Contractile Proteins; D008840:Microfilament Proteins; D051304:Profilins; C050737:iota toxin, Clostridium perfringens; D000246:Adenosine Diphosphate Ribose; D000255:Adenosine Triphosphate; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; D000251:Adenosine Triphosphatases