title: European intercomparison study for the determination of the fumonisins content in two maize materials.
abstract: The results of an intercomparison study for the analysis of fumonisin B1 (FB1) and fumonisin B2 (FB2) in a contaminated maize material (Maize B, containing approximately 2 micrograms/g FB1 and 1 micrograms/g FB2) and its 'blank' counterpart (Maize A, containing less than 20 ng/g FB1 and FB2) are reported. Maize materials were distributed in 60 g sachets, submitted to gamma-irradiation at 15 kGy, and distributed to participating laboratories. The study was carried out by 24 European laboratories, most of which have national or international responsibilities for food/feed quality control. Participants used basically one method with some modifications, based on clean-up through SAX minicolumn and reversed phase HPLC with fluorescence detection of the OPA-fumonisin derivatives. The intercomparison study generated data (after correction for recoveries) with repeatability and reproducibility levels of: RSDr = 10% and RSDR = 11% for FB1; RSDr = 11% and RDSR = 13% for FB2. The recoveries obtained by most participants were considered low (70% for FB1 and 69% for FB2), being considerably affected by the extraction mode. Average recoveries for laboratories using blending were 62% and 60%, whereas for laboratories using shaking they were 85% and 86% for FB1 and FB2, respectively. The day-to-day- data showed a between-day, within-laboratory repeatability lower than 15% (CV < 15%) for FB1 for all laboratories, whereas only three laboratories obtained a between-day, within-laboratory reproducibility higher than 15% (CV > 15%) for FB2.
authors.affiliation: Istituto Tossine e Micotossine da Parassiti Vegetali, Consiglio Nazionale delle Ricerche, Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Visconti
authors.forename: A
authors.initials: A
authors.lastname: Boenke
authors.forename: M
authors.initials: M
authors.lastname: Solfrizzo
authors.forename: M
authors.initials: M
authors.lastname: Pascale
authors.forename: M B
authors.initials: MB
authors.lastname: Doko
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002274:Carcinogens, Environmental / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005060:Europe; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins; D011786:Quality Control; D012015:Reference Standards; D003313:Zea mays
chemical_list: D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; C056934:fumonisin B2; C056933:fumonisin B1