title: The beta-1, 6-glucan containing side-chain of cell wall proteins of Saccharomyces cerevisiae is bound to the glycan core of the GPI moiety.
abstract: Cell wall proteins of Saccharomyces cerevisiae are anchored by means of a beta-1, 6-glucan-containing side-chain. It is not known whether this chain is linked to the protein part (e.g. through carbohydrate side-chains) or to the glycosylphosphatidylinositol (GPI) moiety of cell wall proteins. An IgA protease recognition site was introduced in Cwp2p, a beta-1, 6-glucosylated cell wall protein, immediately N-terminal from the omega amino acid (the attachment site of the GPI moiety). Proteolytic cleavage of this site revealed that the beta-1, 6-glucan epitope was not linked to the protein part. We conclude that neither N-or O-glycosylation is involved in beta-glucosylation of cell wall proteins. This confirms that the glycan core of the GPI moiety is the probable beta-1, 6-glucan attachment site.
authors.affiliation: Department of Molecular Cell Biology, University of Utrecht, The Netherlands. marcel-van-de.vaart@unilever.com
authors.forename: J M
authors.initials: JM
authors.lastname: Van Der Vaart
authors.forename: R
authors.initials: R
authors.lastname: te Biesebeke
authors.forename: J W
authors.initials: JW
authors.lastname: Chapman
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
authors.forename: C T
authors.initials: CT
authors.lastname: Verrips
mesh_terms: D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D017930:Genes, Reporter / Q000502:physiology; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism; D014170:Transformation, Genetic; D000519:alpha-Galactosidase / Q000378:metabolism; D047071:beta-Glucans
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D011134:Polysaccharides; D047071:beta-Glucans; C064197:beta-1,6-glucan; D000519:alpha-Galactosidase; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase