Possible involvement of nitric oxide in chlordiazepoxide-induced feeding in the mouse.
Two experiments investigated a possible role of nitric oxide (NO) in chlordiazepoxide (CP)-induced feeding in nondeprived male ICR mice in independent groups designs. Experiment 1 demonstrated a dose-related decrease in CP-induced solid food intake over a 60-min test period with increasing dose (10, 25, and 50 mg/ kg SC) of the NO-synthase (NOS) inhibitor, L-NG-nitro arginine (L-NOARG), reaching statistical significance at 10 mg/kg L-NOARG when compared to vehicle control. Identical doses of L-NOARG failed to significantly affect normal feeding in vehicle treated mice. In Experiment 2, initial pretreatment with L-arginine (500 and 1000 mg/kg IP) partially or completely restored the feeding inhibitory action of a challenge dose (25 mg/kg SC) of L-NOARG; D-arginine (500 mg/kg IP) was ineffective, thus supporting a stereospecific action of arginine. Arginine isomers did not differentially affect intake in normal feeding animals. These results implicate involvement of NO in CP-induced hyperphagia; they are consistent with and extend research linking NO and ingestive behaviors.
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Department of Psychology, Marquette University, Milwaukee, WI 53201-1881, USA.
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002707:Chlordiazepoxide; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000502:physiology*; D006963:Hyperphagia / Q000139:chemically induced / Q000503:physiopathology*; D007536:Isomerism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000037:antagonists & inhibitors / Q000494:pharmacology
D019335:Nitroarginine; D009569:Nitric Oxide; D002707:Chlordiazepoxide; D001120:Arginine; D019001:Nitric Oxide Synthase