Molecular cloning and characterization of the mouse Kin17 gene coding for a Zn-finger protein that preferentially recognizes bent DNA.
We report the isolation of the mouse Kin17 gene, located on chromosome 2, coding a nuclear Zn-finger protein that has a 39-residue region homologous to Escherichia coli RecA protein and that is recognized by anti-RecA antibodies. Kin17 protein preferentially binds to curved DNA in vitro and in vivo, suggesting a role in illegitimate recombination and in regulation of gene expression. We have shown that the Kin17 gene is about 8 kb in length and displays three exons and two introns. The 5' flanking region lacks a canonical TATAA box but presents several putative regulatory domains. A major transcription initiation site is located 322 nucleotides upstream of the translation start site. The 1.7-kb transcript of the Kin17 gene is weakly and ubiquitously expressed in murine tissues and cell lines as determined by Northern analysis. The cross-hybridization of Kin17 cDNA with the genomic DNA of other species in Southern analysis indicates the conservation of the gene among mammals and suggests that the Kin17 gene plays a conserved role in DNA metabolism.
Tissier
A
A
Département de Radiobiologie et de Radiopathologie, Centre d'Etudes Nucléaires de Fontenay-aux-Roses, Commissariat à l'Energie Atomique, France.
Kannouche
P
P
Mauffrey
P
P
Allemand
I
I
Frelat
G
G
Devoret
R
R
Angulo
J F
JF
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D004285:Dogs; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; C489487:KIN protein, human; C489488:Kin protein, rat; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C071214:Kin protein, mouse; D004247:DNA