Autoantibodies against ADP/ATP carrier from patients with dilated cardiomyopathy increase activity of voltage-dependent Ca channels in isolated cardiac myocytes.
The effects of autoantibodies against the ADP/ATP carrier, from sera of patients with dilated cardiomyopathy, on calcium channel current (ICa) were studied in enzymatically-isolated guinea pig ventricular myocytes by using a whole cell patch-clamp method. The results showed that the autoantibodies enhanced ICa in a concentration-dependent manner. Verapamil inhibited enhancement of ICa induced by the autoantibodies. Control sera (without the autoantibodies) did not affect ICa. This study suggests that anti-ADP/ATP carrier autoantibodies from sera of patients with dilated cardiomyopathy may enhance ICa and cause calcium overload. Disturbing Ca-channel gating by autoantibodies may contribute to the pathogenesis of dilated cardiomyopathy.
Liao
Y H
YH
Institute of Cardiovascular Disease, Xie-he Hospital, Wuhan, China.
Cheng
L X
LX
Dai
S P
SP
Tu
Y S
YS
D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000276:immunology*; D002478:Cells, Cultured; D006168:Guinea Pigs; D000226:Mitochondrial ADP, ATP Translocases / Q000276:immunology*; D009206:Myocardium / Q000378:metabolism*
D001323:Autoantibodies; D015220:Calcium Channels; D000226:Mitochondrial ADP, ATP Translocases; D002118:Calcium