Effect of histone-H2A (H-H2A), platelet activating factor (PAF) and pregnancy specific protein B (PSPB) on secretion of prostaglandins E and F2 alpha (PGE; PGF2 alpha) by bovine endometrium and H-H2A on basal secretion of luteinizing hormone (LH) by bovine pituitary cells in vitro.
Effects of H-H2A, PSPB or PAF on day 16 bovine endometrial secretion of PGE and PGF2 alpha and H-H2A on basal secretion of LH by bovine pituitary cells in vitro were examined in two experiments. PAF (P < or = 0.08) and H-H2a + PAF (P < or = 0.10) treatment for two hours in an in vitro perfusion system tended to increase secretion of PGF2 alpha expressed as a proportion of the prechallenge concentrations of PGF2 alpha by day 16 bovine caruncular endometrium, which occurred during the two-hour period after treatment removal. PGF2 alpha was increased (P < or = 0.02) in the two hour period after the treatment was removed in both control and H-H2A-treated endometrium. H-H2A (P < or = 0.07) and PSPB (P < or = 0.08) tended to increase PGE, while H-H2A + PSPB (P < or = 0.05) and H-H2A + PAF (P < or = 0.03) increased secretion of PGE expressed as a proportion of the prechallenge concentrations of PGE by day 16 bovine caruncular endometrium during the challenge and postchallenge treatment periods, but did not differ (P < or = 0.05) between the two-hour challenge period and the two-hour postchallenge period after removal of the treatment. In experiment two, H-H2A decreased (P < or = 0.05) basal secretion of LH by bovine pituitary cells in vitro. These data suggest that H-H2A and PSPB preferentially stimulate secretion of PGE by bovine endometrial tissue and may play a role in maternal recognition of pregnancy, while PAF increased PGF2 alpha secretion. In addition, H-H2A may play a role in regulating secretion of LH by the pituitary. Key words: Pregnancy Specific Protein B, Histone-H2A, Prostaglandin E and F2 alpha, Platelet Activating Factor, Endometrium, Cow.
Weems
C W
CW
Department of Animal Science, University of Hawaii, Honolulu 96822, USA.
Weems
Y S
YS
French
J T
JT
French
L N
LN
Sasser
R G
RG
Welsh
T H
TH
Harms
P G
PG
Randel
R D
RD
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000494:pharmacology*; D002417:Cattle; D015237:Dinoprost / Q000378:metabolism; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism
D006657:Histones; D010972:Platelet Activating Factor; D011257:Pregnancy Proteins; D011453:Prostaglandins; D011458:Prostaglandins E; D007986:Luteinizing Hormone; D015237:Dinoprost; D016282:Aspartic Acid Endopeptidases; C094766:pregnancy-associated glycoprotein 1