title: [Molecular approach to detection of micrometastatic prostatic cancer cells in the lymph nodes and the bone marrow].
abstract: We detected micrometastatic prostate cancer cells in lymph nodes and bone marrow using reverse transcriptase-polymerase chain reaction (RT-PCT) specific for prostate-specific antigen (PSA). RT-PCR revealed PSA mRNA in two lymph nodes obtained from two patients with negative histological and immunohistochemical analyses for lymph node metastases. Of 26 patients with negative bone scan imaging, 7 had PSA mRNA detected in the bone marrow by RT-PCR. The RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node and bone metastases in patients with prostate cancer.
authors.affiliation: Department of Urology, Gifu University School of Medicine.
authors.forename: T
authors.initials: T
authors.lastname: Deguchi
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Nishino
authors.forename: H
authors.initials: H
authors.lastname: Ehara
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawata
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Doi
mesh_terms: D019046:Bone Marrow Neoplasms / Q000556:secondary*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000235:genetics; D011471:Prostatic Neoplasms / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity
chemical_list: D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D017430:Prostate-Specific Antigen