Mutants of Trichoderma reesei are defective in cellulose induction, but not basal expression of cellulase-encoding genes.
Four mutants of Trichoderma reesei defective in cellulose utilization were characterized at the molecular level. Genomic analysis of the cellulase-encoding genes (cel) and transcript induction using two well-established inducers of the cel system--the insoluble polymer, cellulose and the soluble inducer, sophorose,--revealed that these mutants are defective in the transcription of cel genes. The results also indicate that the cel genes are coordinately expressed and most probably are regulated by the same mechanism. Using a heterologous gene construct, in which the hygromycin-B-resistance-encoding gene was placed under the control of the promoter of the major cel gene, cbh1, we showed that the mutants synthesize basic levels of cellulase, but are defective in the cel induction.
Torigoi
E
E
Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Brazil.
Henrique-Silva
F
F
Escobar-Vera
J
J
Carle-Urioste
J C
JC
Crivellaro
O
O
El-Dorry
H
H
El-Gogary
S
S
D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D002482:Cellulose / Q000096:biosynthesis*; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D002482:Cellulose; D002480:Cellulase