title: The indoor microfungus Trichoderma viride potentiates histamine release from human bronchoalveolar cells.
abstract: Trichoderma viride (Tv) is often found in damp and mouldy buildings where people complain of adverse health effects including mucosal/respiratory symptoms. Inhaled spores can reach the alveoli and may interact with the airway epithelium. An interaction with the mucosal mast cells was studied in cells obtained by bronchoalveolar lavage (BAL) from 18 individuals. The fungal spores were found to trigger histamine release from the BAL cells, but relatively high concentrations (0.1-2 mg/ml) were needed. A similar dose response was obtained in basophil histamine release. The Tv-induced mediator release was caused by non-immunological (non-IgE-dependent) mechanisms since the histamine release was not changed by removal of IgE from the basophils before exposure of the cells to the spores. However, in very low concentrations (0.1 ng/ml) the fungal spores were found to potentiate IgE-mediated histamine release triggered by anti-IgE antibody in suspensions of BAL cells. Potentiation was also obtained in basophil histamine release, but relatively high concentrations of Tv (10(-2) mg/ ml) were needed. Our in vitro experiments show that mucosal mast cells from the airways are highly sensitive to the potentiating effect of Tv. Although inhalation studies are needed to determine the in vivo effect of the spores, the results suggest reinforcement of mediator release to be a mechanism in the adverse health implications observed in mouldy buildings.
authors.affiliation: Department of Pharmacology, University of Copenhagen, Panum Institute, Denmark.
authors.forename: F O
authors.initials: FO
authors.lastname: Larsen
authors.forename: P
authors.initials: P
authors.lastname: Clementsen
authors.forename: M
authors.initials: M
authors.lastname: Hansen
authors.forename: N
authors.initials: N
authors.lastname: Maltbaek
authors.forename: S
authors.initials: S
authors.lastname: Gravesen
authors.forename: P S
authors.initials: PS
authors.lastname: Skov
authors.forename: S
authors.initials: S
authors.lastname: Norn
mesh_terms: D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007476:Ionophores / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology; D008875:Middle Aged; D013172:Spores, Fungal / Q000276:immunology; D014242:Trichoderma / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007476:Ionophores; D007073:Immunoglobulin E