Silver intensification of immunocolloidal gold on ultrathin plastic sections applied to the study of the neuronal distribution of GABA and glycine.
A postembedding silver intensification procedure for immunogold on ultrathin sections has been used to help in the study of localization and co-localization of glycine and GABA in synaptic terminals and cell bodies in the cochlear nucleus of the auditory pathway. Intensification take place in a single step after the immunogold procedure. Intensification times vary from 2 to 6 minutes. This allows visualization of silver enhanced gold particles at low electron microscopic magnifications, which greatly facilitates the analysis of patterns of distribution of putative inhibitory ending immunolabelled for glycine, GABA or both glycine and GABA.
Juiz
J M
JM
Instituto de Neurociencias, University of Alicante, Spain.
Campos
M L
ML
Helfert
R H
RH
Altschuler
R A
RA
D000818:Animals; D017626:Cochlear Nucleus / Q000648:ultrastructure*; D005998:Glycine / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D016622:Silver Staining / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D005998:Glycine