B-1 cell (CD5+B220+) outgrowth in murine schistosomiasis is genetically restricted and is largely due to activation by polylactosamine sugars.
Previously, we demonstrated that lacto-N-fucopentaose III, a sugar found on egg Ags of Schistosoma mansoni, stimulated splenic B cells from parasite-infected mice to proliferate and produce IL-10 and PGE2. The major source of B cell IL-10 is the B-1 subset (CD5+B220+). Thus we examined whether levels of peritoneal exudate B-1 cells changed as a consequence of infection. In CBA/J, BALB/c, and C3H/HeJ mice, we observed significant increases in B-1 cells at 2 to 4 wk postinfection, declining to baseline by 6 to 8 wk. In contrast, the percentage of B-1 cells remained unchanged in C57BL/6 and BALB/c X.id mice after infection. B-1 cells were not observed in the spleens of infected mice; however, coincident with peritoneal B-1 cell decline, splenic CD23+B220+ cells increased from 11% to 30%. Peritoneal B-1 cells could also be expanded by injection of soluble egg Ag, but not by its deglycosylated form, suggesting a role for carbohydrates in B-1 recruitment. In addition, these cells secreted in vitro large amounts of IL-10 in response to lacto-N-fucopentaose III. Further, this sugar induced B-1 cell outgrowth in CBA/J and C3H/HeJ mice, but not in C57BL/6 mice. Thus, early activation of polylactosamine-reactive, IL-10-producing peritoneal B-1 and splenic B cells may be related to early dominance of the Th2-type CD4+ T cell subset. The degree to which this occurs may in part explain differences in the degree of granulomatous pathology reported among various strains of mouse.
Velupillai
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Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115, USA.
Secor
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Horauf
A M
AM
Harn
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D000606:Amino Sugars / Q000494:pharmacology*; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology*; D018956:CD5 Antigens / Q000032:analysis*; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D004768:Enterotoxins / Q000494:pharmacology; D005260:Female; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016513:Mice, SCID; D011134:Polysaccharides / Q000494:pharmacology*; D012555:Schistosomiasis mansoni / Q000235:genetics* / Q000276:immunology*; D013045:Species Specificity; D018089:Superantigens / Q000494:pharmacology
D000606:Amino Sugars; D018956:CD5 Antigens; D004768:Enterotoxins; D011134:Polysaccharides; D018089:Superantigens; C066929:polylactosamine; C026066:enterotoxin A, Staphylococcal; D017493:Leukocyte Common Antigens