Functional expression of the guinea pig 11b-hydroxylase in COS-1 cells.
We expressed a guinea pig 11 beta-hydroxylase cDNA (1) in COS-1 cells. In order to find the optimal expression system we compared three expression plasmids, each driven by a different promoter. Although promoters exhibited different transcriptional activities this did not result in different enzymatic activities. Upon cotransfection with bovine adrenodoxin a 5-fold increase of enzyme activity was achieved. A comparison with the bovine 11 beta-hydroxylase clearly demonstrated that the guinea pig enzyme was not able to produce significant amounts of 18-hydroxylated and 18-oxidized products from deoxycorticosterone under the experimental conditions used.
Bülow
H E
HE
Max-Delbrück-Centrum für Molekulare Medizin, Berlin.
Möbius
K
K
Bähr
V
V
Bernhardt
R
R
D000325:Adrenodoxin / Q000235:genetics; D000818:Animals; D019556:COS Cells; D002417:Cattle; D003900:Desoxycorticosterone / Q000378:metabolism; D015870:Gene Expression; D006168:Guinea Pigs; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D000325:Adrenodoxin; D003900:Desoxycorticosterone; D013252:Steroid 11-beta-Hydroxylase