title: Intratracheal surfactant administration preserves airway compliance during lung reperfusion.
abstract: BACKGROUND
  Decreased airway compliance after lung transplantation has been observed with severe ischemia-reperfusion injury. Further, it has been shown that the surfactant system is impaired after lung preservation and reperfusion. We hypothesized that surfactant replacement after allograft storage could preserve airway compliance during reperfusion.
  
  
  METHODS
  Rabbit lungs were harvested after flush with 50 mL/kg of cold saline solution. Immediate control lungs were studied with an isolated ventilation/perfusion apparatus using venous rabbit blood recirculated at 40 mL/min, room-air ventilation at 20 breaths/min, and constant airway pressure (n = 8). Twenty-four-hour control lungs were preserved at 4 degrees C for 24 hours and then similarly studied (n = 7). Surfactant lungs underwent similar harvest and preservation for 24 hours, but received 1.5 mL/kg of intratracheal surfactant 5 minutes before reperfusion (n = 10). Airway pressure and flow were recorded continuously during 30 minutes of reperfusion. Tidal volume and airway compliance were calculated at 30 minutes.
  
  
  RESULTS
  Tidal volume was 33.67 +/- 0.57, 15.75 +/- 5.72, and 29.83 +/- 1.07 mL in the immediate control, 24-hour control, and surfactant groups, respectively (p = 0.004, surfactant versus 24-hour control). Airway compliance was 1.94 +/- 0.27, 0.70 +/- 0.09, and 1.46 +/- 0.10 mL/mm Hg in the immediate control, 24-hour control, and surfactant groups, respectively (p = 0.002, surfactant versus 24-hour control).
  
  
  CONCLUSIONS
  We conclude that surfactant administration before reperfusion after 24 hours of cold storage preserves tidal volume and airway compliance in the isolated ventilated/perfused rabbit model of lung reperfusion injury.
authors.affiliation: Department of Surgery, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Buchanan
authors.forename: M C
authors.initials: MC
authors.lastname: Mauney
authors.forename: V I
authors.initials: VI
authors.lastname: Parekh
authors.forename: N F
authors.initials: NF
authors.lastname: DeLima
authors.forename: O A
authors.initials: OA
authors.lastname: Binns
authors.forename: J T
authors.initials: JT
authors.lastname: Cope
authors.forename: K S
authors.initials: KS
authors.lastname: Shockey
authors.forename: C G
authors.initials: CG
authors.lastname: Tribble
authors.forename: I L
authors.initials: IL
authors.lastname: Kron
mesh_terms: D000818:Animals; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D008170:Lung Compliance; D016040:Lung Transplantation; D009926:Organ Preservation; D009929:Organ Size; D011663:Pulmonary Surfactants / Q000008:administration & dosage*; D011817:Rabbits; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013990:Tidal Volume; D014132:Trachea; D014655:Vascular Resistance; D014692:Ventilation-Perfusion Ratio
chemical_list: D011663:Pulmonary Surfactants