Ethanol- and diazepam-induced cytochrome oxidase activity in mammillary bodies.
This study aims to analyze the effect of the administration of diazepam and alcohol on cytochrome c oxidase activity (COX) in the mammillary bodies (MB) with a quantitative densitometry method. The histochemical reaction of the COX is used as a reflection of energy consumption. Our results show an increase in the COX activity after treatment with diazepam in the different nuclei of MB: medial medial nucleus (MMNm), lateral medial nucleus (MMN1), and lateral nucleus (LMN) of the MB, the MMNm and LMN being significantly more active compared to the MMN1. Furthermore, the consequences of administering these drugs become manifest in spatial learning (water T maze). The performance in a spatial discrimination task did not prove to be impaired.
Rubio
S
S
Laboratory of Psychobiology, Faculty of Psychology, Oviedo University, Spain.
Begega
A
A
Santín
L J
LJ
Arias
J L
JL
D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D003080:Cold Temperature; D003975:Diazepam / Q000494:pharmacology*; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D018757:GABA Modulators / Q000494:pharmacology*; D006651:Histocytochemistry; D008297:Male; D008326:Mammillary Bodies / Q000187:drug effects / Q000201:enzymology*; D018782:Maze Learning / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
D002492:Central Nervous System Depressants; D018757:GABA Modulators; D000431:Ethanol; D003576:Electron Transport Complex IV; D003975:Diazepam