Correlation between human vascular smooth muscle cell proliferation and protein kinase C alpha-expression: effect of d-alpha-tocopherol.
Population doublings of four different human aortic vascular smooth muscle cell strains correlate with the amount of protein kinase C alpha present in these cells. d-alpha-Tocopherol inhibits, at different extents, protein kinase C activity in all cells studied. Furthermore, the extent of inhibition positively correlates with amount of protein kinase C alpha expression and not with that of the other isoforms. It is suggested that, in human aortic smooth muscle cells, protein kinase C alpha modulates cell proliferation and serves as a target for d-alpha-tocopherol inhibition.
Marilley
D
D
Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
Mosieniak
G
G
Boscoboinik
D
D
Azzi
A
A
D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D014810:Vitamin E / Q000494:pharmacology*; D000099221:Protein Kinase C zeta
D007527:Isoenzymes; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D014810:Vitamin E; D000099221:Protein Kinase C zeta; C496628:PRKCA protein, human; C497258:PRKCD protein, human; C497255:PRKCE protein, human