Cloning and chromosomal localization of the rat Stat5 and Yy1 genes.
Rat beta-casein gene expression is controlled in part by the positively acting factor Stat5 and the negatively acting factor Yy1. Partial cDNAs encoding the SH2 domain of rat Stat5a and Stat5b and the zinc finger domain of rat Yy1 have been cloned by an RT-PCR based approach. The nucleotide sequences encoding the SH2 domain of rat Stat5a and Stat5b are 75% homologous. The rat Yy1 cDNA is 95% homologous to its human and mouse counterparts in the zinc finger domain-encoding region. Using fluorescence in situ hybridization, we have localized Stat5a to rat chromosome band 10q32.1 and Yy1 to rat chromosome band 6q32.
Chen
K S
KS
Department of Human Oncology, University of Wisconsin, Madison 53792, USA.
Paladugu
A
A
Aldaz
C M
CM
Gould
M N
MN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D005796:Genes; D017404:In Situ Hybridization, Fluorescence; D008894:Milk Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011920:Rats, Inbred WF; D011921:Rats, Inbred WKY; D050799:STAT5 Transcription Factor; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D050986:YY1 Transcription Factor; D016335:Zinc Fingers / Q000235:genetics; D018909:src Homology Domains
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D008894:Milk Proteins; D050799:STAT5 Transcription Factor; C494094:Stat5a protein, rat; C494097:Stat5b protein, rat; D015534:Trans-Activators; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494700:Yy1 protein, rat