13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum.
We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using 13C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.
Ferain
T
T
Laboratoire de Génétique Moléculaire, Unité de Génétique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Schanck
A N
AN
Delcour
J
J
D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002247:Carbon Isotopes; D019343:Citric Acid / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D017353:Gene Deletion; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007553:Isotope Labeling; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D045022:Lactate Dehydrogenases; D007778:Lactobacillus / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D019343:Citric Acid; D045022:Lactate Dehydrogenases; D007770:L-Lactate Dehydrogenase; C071195:D-lactate dehydrogenase; D005947:Glucose