Failure of smooth muscle cells treated with collagenase to respond to Bordetella heat-labile toxin.
HLT, bordetella heat-labile toxin, caused a release of radioactivity from cultured smooth muscle cells (SMC) previously labeled with [14C]arachidonic acid, and from membrane preparations from the labeled SMC. HLT, however, failed to induce release from primary SMC prepared by treatment with an enzyme solution containing collagenase and elastase. When cultured SMC were treated with the enzyme solution, their susceptibility to HLT was reduced more significantly than that of untreated cells. SMC also lost their susceptibility, after treatment with collagenase alone, but not with elastase, in a dose-dependent manner. These data suggest that collagen on the SMC surface might play an important role in HLT activity.
Nagai
M
M
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Watanabe
M
M
Endoh
M
M
Danbara
H
H
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects*; D013552:Swine; D010566:Virulence Factors, Bordetella / Q000633:toxicity*
D010566:Virulence Factors, Bordetella; D016718:Arachidonic Acid; D003094:Collagen; D017364:Collagenases