title: Chromosomal changes and progressive tumorigenesis of human bronchial epithelial cell lines.
abstract: A simian virus 40 (SV40)-transformed human bronchial epithelial cell line, BEAS-2B, underwent progressive changes, including the development of tumorigenicity, during extended in vitro passaging. Karyotypic changes occurred in parallel with the phenotypic changes. For the first 12 passages following viral transformation, there were random karyotypic changes. Immortalization occurred between passages 12 and 21, corresponding with the accumulation of four characteristic abnormal chromosomes-m-1: add(15)(p11.1); m-2: der(8;9)(q10;q10); m-3: add(16)(p13); and m-4: mar4- and the loss of one homolog of chromosomes 8, 15, 16, 21, and 22. With further passaging (from 21 to 63), the acquisition of weak tumorigenicity was observed, accompanied by an increased frequency of cells containing all four common abnormal chromosomes, m-1 through m-4, and missing one normal homolog of chromosomes 8, 15, 16, and 22. Four tumor cell lines (B39-TL, B39-TR, B61-T4 and B61-T7) were established from tumors induced by the injection of these weakly tumorigenic BEAS-2B 39th- and 61st- passage cells into athymic nude mice. One of the cell lines, B39-TL, is significantly more tumorigenic than the others. It is notable that B39-TL showed two specific abnormal chromosomes, del(3p);der(3;15) (q10;q10) and m-6; der(21)t(3;21)(p14.2;p12) inducing deletion of a short arm of chromosome 3. Fluorescence in situ hybridization analysis with a probe for protein tyrosine phosphatase-gamma demonstrated loss of heterozygosity in the 3p14 region. The development of step-wise karyotypic changes in this in vitro carcinogenesis model parallels changes documented in several common human cancers.
authors.affiliation: Huntington Medical Research Institutes, Pasadena, California 91101, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnuki
authors.forename: R R
authors.initials: RR
authors.lastname: Reddel
authors.forename: S E
authors.initials: SE
authors.lastname: Bates
authors.forename: T A
authors.initials: TA
authors.lastname: Lehman
authors.forename: J F
authors.initials: JF
authors.lastname: Lechner
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D000818:Animals; D001980:Bronchi; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D002892:Chromosomes, Human, Pair 22; D002898:Chromosomes, Human, Pair 8; D046508:Culture Techniques / Q000379:methods; D004848:Epithelium; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D013539:Simian virus 40; D014183:Transplantation, Heterologous