Effect of estradiol on cytokine secretion by proteolipid protein-specific T cell clones isolated from multiple sclerosis patients and normal control subjects.
The ability of estradiol (E2) to modulate the secretion of cytokines by CD4+ T cells was investigated using neuroantigen-specific T cell clones isolated from normal control subjects and patients with the demyelinating disease, multiple sclerosis. In the presence of E2, the majority of the tested clones showed a dose-dependent enhancement of Ag-stimulated IL-10 secretion. The secretion of IFN-gamma was also enhanced, although the peak enhancement occurred at lower E2 concentrations and at lower magnitude than observed for IL-10. By contrast, the effect of E2 on Ag-stimulated secretion of TNF-alphabeta was biphasic, with enhancement occurring at low doses, and inhibition present at high concentrations. E2 had no effect on the secretion of IL-4 or TGF-beta. These data indicate that E2 is capable of modulating both pro- and anti-inflammatory activities of CD4+ T cells and thus has the potential to influence the outcome of CD4+ T cell-mediated immune responsiveness.
Gilmore
W
W
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90033, USA.
Weiner
L P
LP
Correale
J
J
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002999:Clone Cells; D016207:Cytokines / Q000187:drug effects* / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology* / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000276:immunology*
D016207:Cytokines; D018991:Myelin Proteolipid Protein; D004958:Estradiol