title: Chromaffin granule membrane glycoprotein IV is identical with Ac45, a membrane-integral subunit of the granule's H(+)-ATPase.
abstract: Glycoprotein IV of bovine adrenal chromaffin granule membranes was purified by membrane fractionation with Triton X-114 and lectin affinity chromatography. An antiserum raised against this protein recognized the same component as one directed against subunit Ac45 of the proton-translocating adenosine triphosphatase in the granule membrane. Amino acid sequencing confirmed that glycoprotein IV and Ac45 are identical proteins, and also showed that they are derived from a larger precursor by removal of a 246-amino acid N-terminal sequence. Enzymatic deglycosylation indicated an apparent polypeptide molecular mass of 29 kDa for the mature Ac45/glycoprotein IV. Blue Native electrophoresis confirmed that this protein is a component of the membrane sector of the V-ATPase.
authors.affiliation: Department of Biochemistry, University of Edinburgh, UK.
authors.forename: F
authors.initials: F
authors.lastname: Getlawi
authors.forename: A
authors.initials: A
authors.lastname: Laslop
authors.forename: H
authors.initials: H
authors.lastname: Schägger
authors.forename: J
authors.initials: J
authors.lastname: Ludwig
authors.forename: J
authors.initials: J
authors.lastname: Haywood
authors.forename: D
authors.initials: D
authors.lastname: Apps
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D006180:Proton-Translocating ATPases