Cloning and sequence analysis of the human salivary peroxidase-encoding cDNA.
A cDNA (hSPO) encoding human salivary peroxidase (hSPO) was isolated and its nucleotide (nt) sequence determined. The coding sequence resembles those of other mammalian peroxidases and contains a 3' untranslated region of 538 nt. In a rabbit reticulocyte lysate transcription/translation system, the cDNA produces a major protein of approx. 67 kDa, which corresponds to the calculated molecular weight of unglycosylated hSPO.
Kiser
C
C
Department of Pediatric Dentistry, University of Alabama Schools of Medicine and Dentistry, Birmingham, AL 35294, USA.
Caterina
C K
CK
Engler
J A
JA
Rahemtulla
B
B
Rahemtulla
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D011817:Rabbits; D012469:Salivary Glands / Q000201:enzymology*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D009195:Peroxidase