title: FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.
abstract: We have used mini-Tn1O mutagenesis to identify negative regulators of sigmaD activity. Nine independent insertions were mapped to five genes: flgM, flgK, fliD, fliS, and fliT, suggesting that FlgM export is regulated similarly in Bacillus subtilis and Salmonella typhimurium. We show that a deletion of flgM can restore sigmaD activity to a sinR null mutant of B. subtilis, although fla/che operon expression is affected by neither SinR nor FlgM.
authors.affiliation: Section of Microbiology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: K
authors.initials: K
authors.lastname: Fredrick
authors.forename: J D
authors.initials: JD
authors.lastname: Helmann
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009068:Movement; D016254:Mutagenesis, Insertional; D009876:Operon; D012808:Sigma Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012808:Sigma Factor; C071266:FlgM protein, Bacteria; C076733:FlaD protein, Bacteria; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases