title: [Value of nucleic acid amplification methods Amplicor (Roche) and Amplified MTD (Gen-Probe) for the rapid diagnosis of tuberculosis].
abstract: The nucleic acid amplification methods: Amplicor (Roche diagnostic) and AMTD Amplified Mycobacterium tuberculosis Test Direct-(Gen-Probe) were tested in 278 specimens from 231 patients suspect to be affected by mycobacterial infection. When results of both methods: Amplicor and AMTD were compared with culture results (specimens grow M tuberculosis) and clinical characteristics, the sensitivity and specificity were 91.4% and 97.9% respectively for pulmonary specimens and 61.1% and 98.6% respectively for extrapulmonary specimens. Detection of amplification inhibitors reduce false-negative reactions and control of specimen with microscopic negative and amplification positive, reduce the false-positive reactions. Amplicor and AMTD kits can be used in clinical laboratories. Both assays have the potential to reduce the time of tuberculosis diagnosis to one day.
authors.affiliation: Laboratoire de microbiologie, CHU Nancy-Brabois, Vandoeuvre-lès-Nancy, France.
authors.forename: M
authors.initials: M
authors.lastname: Dailloux
authors.forename: C
authors.initials: C
authors.lastname: Laurain
mesh_terms: D001431:Bacteriological Techniques; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
vernacular_title: Intérêt des méthodes d'amplification d'acides nucléiques Amplicor (Roche) et Amplified MTD (Gen-Probe) pour le diagnostic rapide de la tuberculose.