The DNA binding affinity of rat liver nucleoproteins to the alpha 1-acid glycoprotein gene.
Transcriptional regulation and binding interactions between soluble nucleoproteins and the distal regulatory element (DRE) of the rat alpha 1-acid glycoprotein (alpha 1-AGP) gene were examined in the liver of rats during the acute-phase response. Our results show that the elevation of the alpha1-AGP gene transcription activity in acute phase liver relies basically on an increase in the binding-affinity of the constitutive soluble nucleoproteins with molecular masses 35 and 45 kD, enhancing their capability to bind to the distal regulatory element (DRE) of alpha-AGP gene. On the basis of in vitro phosphorylation/dephosphorylation experiments we discuss that the role of these proteins during alpha 1-AGP transcription may be dependent on their behavior as phosphoproteins. The 35kD nucleoprotein that displayed an acute-phase inducible affinity to bind DRE was identified as a C/EBP beta isoform.
Petrović
M
M
Molecular Biology Laboratory, Institute for Biological Research, Belgrade, Yugoslav Federal Republic.
Grigorov
L
L
Milosavljević
T
T
Bogojević
D
D
D000818:Animals; D001345:Autoradiography; D022762:CCAAT-Enhancer-Binding Proteins; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007123:Immunoelectrophoresis, Two-Dimensional; D008099:Liver / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009698:Nucleoproteins; D014157:Transcription Factors; D004247:DNA