Receptor protein tyrosine kinase DDR is up-regulated by p53 protein.
We have previously reported on radiation-induction of ptk-3 in rat astrocyte culture [Sakuma et al. (1995) Radiat. Res. 143, 1-7]. Ptk-3 was considered to be a rat version of human DDR (discoidin domain receptor). We cloned and analyzed genomic DNA of the DDR and its promoter region. We discovered that the promoter region contained a consensus sequence of the p53 tumor suppressor binding site. Adenovirus-mediated p53 transfection induced a high level of DDR mRNA in SAOS2 human osteosarcoma cells. These results indicate that DDR is up-regulated by the p53 protein.
Sakuma
S
S
Department of Neurosurgery, University of Hokkaido School of Medicine, Kita-ku, Sapporo, Japan. ssakuma@med.hokudai.ac.jp
Saya
H
H
Tada
M
M
Nakao
M
M
Fujiwara
T
T
Roth
J A
JA
Sawamura
Y
Y
Shinohe
Y
Y
Abe
H
H
D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000070818:Discoidin Domain Receptors; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D011975:Receptors, Mitogen / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D015854:Up-Regulation
D011975:Receptors, Mitogen; D016159:Tumor Suppressor Protein p53; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases