[Mechanisms of metabolic changes after in-line filtration of erythrocytes].
BACKGROUND
The reasons for the improved RBC storage after in-line filtration are not fully understood.


METHODS
Red blood cell concentrates were in-line filtered and stored for 6 weeks at 4 degrees C in SAGM. Parameter 2,3-DPG, p50, density, cell electrophoresis, pH were measured.


CONCLUSIONS
The obtained results may explain the metabolic findings: Cell retention (during filtration older, rigide RBC are retained in the filter- a relative increase of younger RBC); changes in RBC membrane permeability (a changed electrophoretic mobility is attributed to bound RBC membrane proteins to the filter material); blood cell depletion (a high depletion efficiency prevents toxic influences of degenerating/disintegrating WBC and PLT); release of substances from filter and blood bag material (during passage of anticoagulated blood some compounds may be released in ppm concentrations - the density of in-line filtered RBC is higher than unfiltered RBC after filtration procedure and hypothermic storage).
Matthes
G
G
Institut für Transfusionsmedizin, Humboldt-Universität zu Berlin, Deutschland.
Bäumler
H
H
Krause
K P
KP
Tofoté
U
U
Knippel
W
W
Budde
A
A
Donath
E
E
Kiesewetter
H
H
D019794:2,3-Diphosphoglycerate; D001771:Blood Banks; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood; D017707:Erythrocyte Transfusion / Q000295:instrumentation*; D004912:Erythrocytes / Q000378:metabolism*; D006452:Hemoglobinometry; D006801:Humans; D008212:Lymphocyte Depletion / Q000295:instrumentation*; D009996:Osmotic Fragility / Q000502:physiology
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate
Mechanismen der metabolischen Veränderungen nach einer In-Line-Filtration von roten Blutzellen.