[Identification and classification of strains of microorganisms using genomic fingerprinting with biotinylated phage M13 DNA].
To analyze DNA polymorphisms of various bacterial strains, a nonradioactive variant of the genomic fingerprinting method was developed. The method was based on the application of biotin-labeled single-stranded phage M13 DNA as a probe. Characteristic patterns of fingerprints obtained by MvaI, HaeIII, and HinfI restriction enzymes are presented for several species of bacilli and other bacteria. The advantages of this method in microbiology for the identification and characterization of different microbial strains are shown.
Oreshkova
S F
SF
Manokhina
O V
OV
Puchkova
L I
LI
Repin
V E
VE
Il'ichev
A A
AA
D001407:Bacillus / Q000235:genetics; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D017104:Bacteriophage M13 / Q000235:genetics*; D001710:Biotin; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics; D016680:Genome, Bacterial; D014733:Vibrio / Q000235:genetics
D015342:DNA Probes; D004279:DNA, Viral; D001710:Biotin
Identifikatsiia i pasportizatsiia shtammov mikroorganizmov metodom genomnoĭ daktiloskopii s ispol'zovaniem biotinilirovannoĭ DNK faga M13.