Differential histochemical peanut agglutinin stain in benign and malignant human prostate tumors: relationship with prostatic specific antigen immunostain and nuclear DNA content.
The histochemical binding pattern of the peanut (Arachis hypogaea) lectin (PNA) was quantitatively described by means of computer-assisted microscope analysis in 28 benign prostatic hyperplasias (BPH), 15 prostatic intraepithelial neoplasias (PIN), and 119 prostatic adenocarcinomas. PNA exhibits nonimmune but selective binding to glycoproteins with beta-D-galactosyl(1,3)-N-acetyl-D-galactosamine residues. We also investigated whether a relationship existed between the number of histochemical-related PNA acceptors and the histochemical prostate-specific antigen (PSA) stain intensity, and between the number of PNA receptors and DNA ploidy level. The results show that neoplastic prostate tissues and high-grade intraepithelial prostatic neoplasias (PIN2_3) exhibit a significantly higher number of PNA acceptors than benign prostatic hyperplasias and low (PIN1) grade prostatic intraepithelial neoplasias. A statistically significant correlation was observed between the number of histochemically related PNA acceptors and PSA immunostain intensity. Lastly, diploid prostatic tumors, whether benign or malignant, exhibited a significantly higher number of PNA acceptors than aneuploid ones. These results suggest that PNA acceptors play an important role in the biology of prostate tumors.
Janssen
T
T
Department of Urology, University Clinics of Brussels, Erasme Hospital, Belgium.
Petein
M
M
Van Velthoven
R
R
Van Leer
P
P
Fourmarier
M
M
Vanegas
J P
JP
Danguy
A
A
Schulman
C
C
Pasteels
J L
JL
Kiss
R
R
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006651:Histocytochemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D037102:Lectins; D008297:Male; D019887:Peanut Agglutinin; D011003:Ploidies; D017430:Prostate-Specific Antigen / Q000032:analysis*; D011470:Prostatic Hyperplasia / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000737:chemistry / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000473:pathology
D004396:Coloring Agents; D037102:Lectins; D019887:Peanut Agglutinin; D004247:DNA; D017430:Prostate-Specific Antigen