Fish testicular 11beta-hydroxylase: cDNA cloning and mRNA expression during spermatogenesis.
We isolated and characterized a cDNA encoding testicular 11beta-hydroxylase, cytochrome P450(11beta) from the Japanese eel (Anguilla japonica) testis. The cDNA contains an open reading frame that encodes a protein of 511 amino acids. The predicted amino acid sequence shares 38-48% homology with those of adrenal P450(11beta) from mammals and frog. Transient expression in COS 1 cells confirmed that the protein encoded by this cDNA had P450(11beta) activity. Northern blotting revealed a single 1.8 kb long transcript of P450(11beta). This transcript was not found in immature eel testes prior to an injection with human chorionic gonadotropin (hCG), but it was present in eel testes after hCG injection.
Jiang
J Q
JQ
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
Kobayashi
T
T
Ge
W
W
Kobayashi
H
H
Tanaka
M
M
Okamoto
M
M
Nonaka
Y
Y
Nagahama
Y
Y
D000595:Amino Acid Sequence; D000735:Androstenedione / Q000378:metabolism; D000811:Anguilla / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis; D013252:Steroid 11-beta-Hydroxylase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000201:enzymology*; D013739:Testosterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D018076:DNA, Complementary; D012333:RNA, Messenger; D013739:Testosterone; D000735:Androstenedione; D013252:Steroid 11-beta-Hydroxylase