Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.
Chlamydia trachomatis RNA polymerase was partially purified by heparin-agarose chromatography and used in conjunction with a plasmid-borne G-less cassette template to characterize the C. trachomatis rRNA P1 promoter in vitro. Stepwise mutational analysis revealed that sequences in the -10, -25, and -35 regions are necessary for promoter activity, but no sequence upstream of position -40 is required. Partially purified C. trachomatis RNA polymerase and purified Escherichia coli holoenzyme exhibited some differences in promoter specificity.
Tan
M
M
Department of Medicine, University of California, San Francisco, 94143-0654, USA.
Engel
J N
JN
D001482:Base Composition; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000235:genetics*; D002847:Chromatography, Agarose; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009876:Operon; D012329:RNA, Bacterial / Q000235:genetics; D017384:Sequence Deletion; D013698:Templates, Genetic; D014158:Transcription, Genetic; D012407:rRNA Operon / Q000235:genetics*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases