[Antigenic structure of the Pro-rich region 536-566 of Bordetella pertussis pertactin (P.69). II. Analysis of peptide-specific antibodies].
New method of analysis of subpopulations of peptide-specific polyclonal antibodies (Abs) without their elimination was developed which does not require radiolabeling. B-epitopes were analyzed which were recognized by rabbit Abs in synthetic peptides KAPPAPKPAPQPGPQPP (17P), QPPQPQPEAPAPQP (14P), and GPQPPQPPQP (10P) from Pro-rich region 536-566 of Bordetella pertussis pertactin. Antigenic structure was studied by indirect and competitive ELISA, immunoaffinity chromatography, isoelectric focusing, and immunoblotting. Abs against all peptides recognize native pertactin. Abs against 14P recognize antigenic determinant in region QPEPAPQP and they comprise two main subpopulations of Abs. Abs against 10P interact with two overlapping B-epitopes GPQPPQPPQP and QPPQP. Abs against 17P recognize an epitope in region KAPPAPKPAPQ and two additional overlapping antigenic determinants PGPQPP and PQPP. Minimal sequence recognized by Abs against P17 is PQPP but not QPP. Abs against 17P comprise approximately 20 major subpopulations; six or seven of these subpopulations effectively interact with the C-terminal region PGPQPP (6P).
Kavun
E M
EM
Borisova
E G
EG
Kolibo
D V
DV
Berezhnoĭ
G A
GA
Makarov
E A
EA
Radavskiĭ
Iu L
IuL
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001886:Bordetella pertussis / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011392:Proline / Q000737:chemistry*; D011817:Rabbits; D010566:Virulence Factors, Bordetella
D000906:Antibodies; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D010566:Virulence Factors, Bordetella; C068929:pertactin; D011392:Proline
Antigennoe stroenie Pro-bogatogo uchastka 536-566 pertaktina (P.69) Bordetella pertussis. II. Analiz peptid-spetsifichnykh antitel.