Inhalation teratology and reproduction studies with 1,1-dichloro-2,2,2-trifluoroethane (HCFC-123).
HCFC 123 is one of the chemicals being developed as a replacement for CFC 11 in refrigerant and solvent applications. Supplementing earlier rat teratology studies, a rabbit inhalation teratology study was conducted. In addition, one-generation and two-generation inhalation reproduction studies were conducted. In the teratology study, the pregnant rabbits were exposed to levels of 0 (control), 500, 1500, and 5000 ppm, 6 hr per day from Days 6 through 18 of gestation. Slight body weight losses and reduced food consumption were seen in does in all three exposure level groups. This response followed an exposure-related pattern. There were no other signs of maternal toxicity. There was also no evidence of treatment-related effects on the kits. A probe one-generation reproduction study was conducted. In this study four groups of 12 male and 12 female rats were exposed to vapors of HCFC 123 6 hr per day, 7 days per week from 4 weeks prior to mating through weaning of their offspring. The exposure levels for this study were 0 (control), 300, 1000, and 5000 ppm. There were no effects on mating and fertility, or on pup survival or birth weight. A two-generation study was subsequently conducted. In this study, five groups of 32 male and female rats were exposed to HCFC 123 from 6 weeks of age through weaning. From the offspring of these animals, groups of 28 males and females were selected for the F1 generation. These animals were exposed to HCFC 123 from weaning (4 weeks of age) through weaning of the F1 generation. All exposures were 6 hr per day, 7 days per week. The exposure levels for this study were 0 (control), 30, 100, 300, and 1000 ppm. There were no effects on any of the fertility or reproductive indices measured. As with prior studies, decreases in serum triglyceride levels were seen. Pup survival and birth weight were unaffected by treatment. Pup body weight gain was lower in all treatment groups during nursing, following an exposure-related pattern. Since weight gain for the F1 animals was normal following weaning, this depression of body weight gain may be related to the depression of serum triglycerides. In addition, liver weights of the adult rats exposed to levels of 100 ppm and higher of HCFC 123 were higher than controls, histological examination revealed only hepatic enlargement and vacuolation. It was concluded that exposure to HCFC 123 did not cause reproductive effects although it did effect the body weight gain of the offspring during lactation.
Malinverno
G
G
Ausimont CRS, Via San Pietro 50, Bollate, 20021, Italy.
Rusch
G M
GM
Millischer
R J
RJ
Hughes
E W
EW
Schroeder
R E
RE
Coombs
D W
DW
D000014:Abnormalities, Drug-Induced / Q000473:pathology*; D000280:Administration, Inhalation; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D017402:Chlorofluorocarbons / Q000008:administration & dosage / Q000633:toxicity*; D064227:Chlorofluorocarbons, Ethane; D005260:Female; D006728:Hormones / Q000097:blood; D007984:Leydig Cell Tumor / Q000139:chemically induced / Q000473:pathology; D008098:Litter Size / Q000187:drug effects; D008297:Male; D018811:Maternal Exposure; D018812:Paternal Exposure; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D012741:Sexual Maturation / Q000187:drug effects; D013045:Species Specificity
D017402:Chlorofluorocarbons; D064227:Chlorofluorocarbons, Ethane; D006728:Hormones; C067411:2,2-dichloro-1,1,1-trifluoroethane