Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay.
A rapid multiplex PCR assay was developed to distinguish between North American and European genotypes of porcine reproductive and respiratory syndrome (PRRS) virus after a portion of the polymerase gene (open reading frame 1b) was sequenced for two North American PRRS virus strains. DNA products with unique sizes characteristic of each genotype were obtained.
Gilbert
S A
SA
Animal Diseases Research Institute, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.
Larochelle
R
R
Magar
R
R
Cho
H J
HJ
Deregt
D
D
D000818:Animals; D001483:Base Sequence; D005060:Europe; D005838:Genotype; D008969:Molecular Sequence Data; D009656:North America; D016133:Polymerase Chain Reaction; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine / Q000821:virology*