title: Autoantibodies to double-stranded (ds)DNA immunoprecipitate 18S ribosomal RNA by virtue of their interaction with ribosomal protein S1 and suppress in vitro protein synthesis.
abstract: We report that four systemic lupus erythematosus (SLE) patient sera containing anti-dsDNA antibodies, three affinity-purified anti-dsDNA IgG, and a human anti-dsDNA MoAb (33.H11) immunoprecipitate 18S ribosomal RNA from DNase-treated 32P-labelled MOLT4 cell extract. This 18S RNA precipitation was inhibited completely by preincubating 33.H11 with calf thymus dsDNA or the recombinant human ribosomal protein S1, which was reported to cross-react with anti-dsDNA antibodies (J Immunol 1996; 156:1668-75). Whole IgG from three SLE sera with anti-dsDNA antibodies, 33.H11, and three affinity-purified anti-dsDNA IgG inhibited in vitro translation of globin mRNA (percent inhibition was 36-50%). This translation inhibition by anti-dsDNA antibodies was enhanced (67-79%) when the reticulocyte lysate was treated with DNase. Suppression of protein synthesis could be a pathogenic mechanism of anti-dsDNA antibodies, since it has also been shown that anti-dsDNA penetrates living cells (J Immunol 1995; 154:4857-64) in culture.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma University Health Sciences Centre, Oklahoma City 73104, USA.
authors.forename: K
authors.initials: K
authors.lastname: Tsuzaka
authors.forename: T H
authors.initials: TH
authors.lastname: Winkler
authors.forename: J R
authors.initials: JR
authors.lastname: Kalden
authors.forename: M
authors.initials: M
authors.lastname: Reichlin
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000494:pharmacology*; D002460:Cell Line; D003429:Cross Reactions / Q000276:immunology; D004247:DNA / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000276:immunology* / Q000378:metabolism*; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects*; D012337:RNA, Ribosomal, 18S / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism; D013491:Suppressor Factors, Immunologic / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D011506:Proteins; D012337:RNA, Ribosomal, 18S; D012261:Ribonucleoproteins; D013491:Suppressor Factors, Immunologic; D004247:DNA