title: Platelet-surface glycoproteins in healthy and preeclamptic mothers and their newborn infants.
abstract: Preeclampsia, a common complication of pregnancy, contributes significantly to maternal and fetal morbidity and mortality. It may lead to both quantitative and qualitative defects of maternal and neonatal platelets. In this prospective study, flow cytometry has been used to study expression of platelet-surface glycoproteins (GPs) on maternal and neonatal platelets of both healthy and preeclamptic subjects. We studied 15 preeclamptic women, 20-44 y of age, and their newborns (median gestational age, 32 wk; range, 26-38) and seven healthy women (aged 26-41 y) and their healthy newborns (median gestational age, 38 wk; range, 38-42). Compared with their healthy and preeclamptic mothers, resting platelets from neonates expressed significantly less CD41 and CD9. Thrombin activation resulted in significant increases in platelet-surface expression of CD62P, CD63, CD41, CD9, and CD36 in neonates and their healthy mothers. Compared with neonates of healthy mothers, platelets from neonates of preeclamptic mothers expressed lower levels of CD62P, CD63, CD9, and CD36 on activated platelets. These findings suggest that preeclampsia influences the expression of platelet-surface GPs on neonatal and maternal platelets, which may affect platelet function, leading to an additional risk for bleeding in thrombocytopenic neonates of mothers with preeclampsia.
authors.affiliation: Division of Hematology, St Michael's Hospital, Toronto, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Kühne
authors.forename: G
authors.initials: G
authors.lastname: Ryan
authors.forename: V
authors.initials: V
authors.lastname: Blanchette
authors.forename: J W
authors.initials: JW
authors.lastname: Semple
authors.forename: A
authors.initials: A
authors.lastname: Hornstein
authors.forename: M
authors.initials: M
authors.lastname: Mody
authors.forename: W
authors.initials: W
authors.lastname: Chang
authors.forename: L
authors.initials: L
authors.lastname: McWhirter
authors.forename: J
authors.initials: J
authors.lastname: Freedman
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis / Q000097:blood*; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology; D018955:CD36 Antigens / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D007234:Infant, Premature; D008297:Male; D008562:Membrane Glycoproteins; D019007:P-Selectin / Q000097:blood; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D012016:Reference Values; D060245:Tetraspanin 29; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; D018955:CD36 Antigens; C554586:CD63 protein, human; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D060245:Tetraspanin 29; D060149:Tetraspanin 30