Cloning and characterization of Xenopus laevis xSox7 cDNA.
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shif assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
Shiozawa
M
M
Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
Hiraoka
Y
Y
Komatsu
N
N
Ogawa
M
M
Sakai
Y
Y
Aiso
S
S
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D055760:SOXF Transcription Factors; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D055760:SOXF Transcription Factors; C484457:Sox7 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins