The cells of monocyte-macrophage lineage in mice with the 2-month polyethylene implantation.
End-point-attached heparinized polyethylene (H-PE) was implanted for 2 months into the peritoneum of C57B1/6 mice. The proliferation of bone marrow cells (BMCs) from implanted and non-implanted mice was investigated in M-CSF supplemented medium, in the presence or absence of macrophage-specific monoclonal antibodies (mAbs). The mAb HC 7.67.B, recognizing a surface determinant on immature monocytoid cells, inhibited the proliferation of BMCs from H-PE implanted mice without any influence on the proliferation of BMCs from non-implanted animals. The peritoneal macrophages from H-PE implanted mice demonstrated enhanced production of fibronectin (Fn) in comparison to the macrophages from non-implanted animals. Our results suggest changes in the differentiation of murine monocyte-macrophage lineage in the mice bearing H-PE implants for 2 months.
Rózalska
B
B
Department of Infectious Biology, University of Lódź, Poland.
Długoñska
H
H
Ljungh
A
A
Paziak-Domañska
B
B
Burow
A
A
Rudnicka
W
W
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects*; D010529:Peritoneal Cavity; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology*; D019736:Prostheses and Implants
D001672:Biocompatible Materials; D011095:Polyethylenes