Molecular cloning and nucleotide sequence of the gene encoding phosphate-inducible pectin lyase of Bacillus subtilis.
The gene encoding the pectin lyase (PNL; EC 4.2.2.10) of Bacillus subtilis has been cloned, sequenced, and characterized. A coding sequence for the PNL composed of 345 amino acids including a 24-amino-acid signal peptide was assigned. No sequence resembling a LexA binding site was found upstream of the structural gene. Furthermore, PNL activity of the gene product expressed in Escherichia coli DH5alpha was detected intracellularly, which might suggest that expression of the gene was not controlled by RecA. Regulation of the gene expression seemed to be quite different from that of other bacterial PNL genes previously reported.
Sakamoto
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T
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, Sakai, Japan. sakamoto@envi.osakafu-u.ac.jp
Kawasaki
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H
Sakai
T
T
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010368:Pectins / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017680:Potassium Compounds / Q000494:pharmacology; D021382:Protein Sorting Signals / Q000737:chemistry; D011938:Rec A Recombinases / Q000378:metabolism; D013696:Temperature
D010710:Phosphates; D017680:Potassium Compounds; D021382:Protein Sorting Signals; D010368:Pectins; C013216:potassium phosphate; D011938:Rec A Recombinases; D011133:Polysaccharide-Lyases; C027349:pectin lyase