[Analytical characteristics of commercial IRMA-AFP kit for determination of human alpha-fetoprotein].
Commercial polyclonal IRMA-AFP kit is manufactured by Radiopreparat in Tashkent since 1986. The kit has a range of 0 to 320 ng/ml. This report includes the data on AFP measurements in the sera of cancer patients, particularly at the lower part of the calibration curve. Within run precision studies yielded a CV of 7.7% at the 4.3-54.0 ng/ml interval. Between run precision was 8.6% at the 3.3-56.0 ng/ml interval. The recovery of AFP in the presence of matrix effects of 9 sera was 98% for mixtures at the 9.1-60.8 ng/ml range. The assay sensitivity was 0.6 ng/ml. In donor blood samples the AFP levels did not exceed 4 ng/ml.
Andreev
A V
AV
Grigor'eva
E Iu
EIu
Gusev
A I
AI
D001782:Blood Donors; D006801:Humans; D015592:Immunoradiometric Assay; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins
Analiticheskie kharakateristiki kommercheskogo nabora IRMA-AFP dlia opredeleniia al'fa-fetoproteina cheloveka.