title: [Stabilization of the alpha-fetoprotein structure with sucrose].
abstract: By means of scanning microcalorimetry and fluorescent spectroscopy, the addition of sucrose was shown to stabilize the structure of human alpha-fetoprotein (AFP). The stabilizing effect was not eliminated during eight-day dialysis of AFP against a buffer containing no sucrose, but it can be substantially weakened by treating AFP with a specific enzyme, invertase, which splits sucrose into fructose and glucose. This indicates that human AFP is capable of specific sucrose binding.
authors.forename: M D
authors.initials: MD
authors.lastname: Kirkitadze
authors.forename: N V
authors.initials: NV
authors.lastname: Narizhieva
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Tomashevskiĭ
authors.forename: S A
authors.initials: SA
authors.lastname: Potekhin
authors.forename: V N
authors.initials: VN
authors.lastname: Uverskiĭ
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013395:Sucrose / Q000737:chemistry*; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry*
chemical_list: D000509:alpha-Fetoproteins; D013395:Sucrose; D000244:Adenosine Diphosphate; D014508:Urea
vernacular_title: Stabilizatsiia struktury alpha-fetoproteina sakharozoĭ.