title: Immunomodulatory action of levofloxacin on cytokine production by human peripheral blood mononuclear cells.
abstract: Levofloxacin (LVFX), the bacteriologically active isomer of ofloxacin, is a fluorinated quinolone. LVFX suppressed the proliferative activity of peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin (PHA). LVFX increased interleukin-2 (IL-2) production by PBMC stimulated with PHA in a dose-dependent manner, with more than 10 micrograms/ml of LVFX causing a significant increase. The granulocyte-macrophage colony-stimulating factor and soluble IL-2 receptor production by PHA-stimulated PBMC was suppressed at high concentrations of LVFX. Interleukin-1 beta production by lipopolysaccharide-stimulated PBMC was suppressed in a concentration-dependent manner by LVFX, and tumor necrosis factor-alpha production was suppressed at only the highest concentration. In contrast, interleukin-8 production was little affected by LVFX. These results show that LVFX has an immunomodulatory action on cytokines production by PBMC independent of its antimicrobial activity.
authors.affiliation: Pharmaceutical Division, Ogaki Municipal Hospital, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: C
authors.initials: C
authors.lastname: Kurita
authors.forename: E
authors.initials: E
authors.lastname: Usami
authors.forename: T
authors.initials: T
authors.lastname: Nakao
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: J
authors.initials: J
authors.lastname: Kobayashi
authors.forename: F
authors.initials: F
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Nagai
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D064704:Levofloxacin; D015242:Ofloxacin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D000890:Anti-Infective Agents; D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D064704:Levofloxacin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015242:Ofloxacin