Identification of apoptotic hepatocytes in situ in rat liver after lead nitrate administration.
Apoptosis plays a major role in the regression of mitogen (lead nitrate)-induced hepatic hyperplasia. We compared the in situ end-labeling (ISEL) technique with the conventional detection of apoptotic bodies in this process. In hematoxylin and eosin (H&E) sections, apoptosis is usually recognizable by the presence of apoptotic bodies (apoptosis phase 2). Although the early phase of apoptosis (apoptosis phase 1) can be detected as a prekaryorrhectic appearance in H&E sections, it is difficult to detect and is easily overlooked. On the other hand, ISEL presents intense staining mainly in phase 1 and weak or negative staining in phase 2. Thus, simultaneous investigation by these two methods in two serial sections is the most reliable way to calculate the incidence of apoptosis and gives us precise information on the stages of apoptosis in situ. Since the colorized signals of ISEL are much easier to detect than apoptotic bodies in H&E sections, ISEL is particularly useful for liver tissues, where the incidence of apoptosis is low.
Nakajima
T
T
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Deguchi
T
T
Kagawa
K
K
Hikita
H
H
Ueda
K
K
Katagishi
T
T
Ohkawara
T
T
Kakusui
M
M
Kimura
H
H
Okanoue
T
T
D000818:Animals; D017209:Apoptosis; D004396:Coloring Agents; D004249:DNA Damage; D003856:Deoxyuracil Nucleotides; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006965:Hyperplasia / Q000139:chemically induced; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization / Q000379:methods; D007854:Lead / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D009566:Nitrates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; D003856:Deoxyuracil Nucleotides; D008934:Mitogens; D009566:Nitrates; C027078:deoxyuridine triphosphate; D007854:Lead; C017461:lead nitrate; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin