Mechanism of action of hemolysin III from Bacillus cereus.
Bacillus cereus hemolysin III activity was tested in crude extracts, from Escherichia coli carrying the hly-III gene. It was concluded that hemolysin III is a pore-forming hemolysin with functional pore diameter of about 3-3.5 nm. Hemolysis occurs in at least three steps: (i) the temperature-dependent binding of the Hly-III monomers to the erythrocyte membrane; (ii) the temperature-dependent formation of the transmembrane oligomeric pore; (iii) the temperature-independent erythrocyte lysis.
Baida
G E
GE
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. gleb@ibpm.serpukhov.su
Kuzmin
N P
NP
D001409:Bacillus cereus; D003001:Cloning, Molecular; D004910:Erythrocyte Membrane / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D009994:Osmolar Concentration; D011485:Protein Binding; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D013696:Temperature
D006460:Hemolysin Proteins; D011994:Recombinant Proteins