Pseudo-transposition of a Tn5 derivative in Neisseria gonorrhoeae.
We constructed a Tn5 derivative for potential use in transposon mutagenesis of Neisseria gonorrhoeae. It was incorporated into the chromosome apparently at random following transformation, but the insertion events were dependent on a functional RecA and independent of a functional transposase. Furthermore, in most cases there was an incomplete transposon inserted with little or no IS50 insertion sequence. These observations suggest that Tn5 transposition may not be possible in N. gonorrhoeae and that this organism may have an unexplored illegitimate recombination system.
Thomas
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CE
Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill 27599, USA. cet@med.unc.edu
Carbonetti
N H
NH
Sparling
P F
PF
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011938:Rec A Recombinases