title: Enhancement of intestinal eosinophilia during Hymenolepis nana infection in mice.
abstract: The ability of Hymenolepis nana oncosphere extract to induce eosinophil chemotactic response was examined in vitro and in vivo. The extract showed a chemotactic activity specific for eosinophils but not for neutrophils. Partially purified eosinophil chemotactic factors (ECFs) from the oncosphere extract showed apparent molecular mass from 5.5 to 9.6kDa and 30 to 40kDa. These were resistant to heating and proteinase K digestion but sensitive to periodate oxidation. Peritoneal injection of the crude extract or partially purified ECFs to mice resulted in a preferential eosinophil infiltration. The chemotactic activity for eosinophils was not separable from the adhesion molecule expression or oxygen radical-inducing activity by means of chromatography or chemical treatments. Furthermore, histological examination demonstrated a marked tissue eosinophilia around H. nana larvae in the intestinal lamina propria of both humoral and cell-mediated immunodeficiency mice. The present findings suggest that H. nana oncosphere-derived molecules facilitate in vivo the intestinal eosinophilia during the infection.
authors.affiliation: Department of Parasitology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Niwa
authors.forename: T
authors.initials: T
authors.lastname: Miyazato
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002631:Chemotactic Factors, Eosinophil / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006925:Hymenolepiasis / Q000276:immunology*; D006926:Hymenolepis / Q000276:immunology; D007411:Intestinal Diseases, Parasitic / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008871:Microvilli / Q000469:parasitology / Q000473:pathology; D010100:Oxygen / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D002631:Chemotactic Factors, Eosinophil; D005609:Free Radicals; D010100:Oxygen