Genomic organization and promoter analysis of the gene ifngr2 encoding the second chain of the mouse interferon-gamma receptor.
A clone containing the gene ifngr2 for the second chain (IFN-gamma R2) of the mouse interferon gamma receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NF kappa B. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells.
Ebensperger
C
C
F.Hoffmann-La Roche AG, Basel, Switzerland.
Rhee
S
S
Muthukumaran
G
G
Lembo
D
D
Donnelly
R
R
Pestka
S
S
Dembic
Z
Z
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005091:Exons; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017471:Receptors, Interferon / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D000096743:Interferon gamma Receptor
D015703:Antigens, CD; D004247:DNA; D017471:Receptors, Interferon; D014157:Transcription Factors; D000096743:Interferon gamma Receptor