title: [Kinetics of in vitro bactericidal activity of the antiseptic biseptine combining 3 active principles].
abstract: Biseptine, is an association of chlorhexidine digluconate, benzalkonium chloride and benzylic alcohol. Little is known, in literature, on this antiseptic, used for cutaneous antisepsis. We studied killing kinetic of Biseptine, at different concentrations, on 4 AFNOR bacterial strains. Killing curves were studied at antiseptic concentration of 90 to 0.1% in 10 ml of distilled water. Bacterial counts were determined after neutralization in liquid medium. Synergy of chlorhexidine and benzalkonium chloride in Biseptine, allowed to obtain similar bactericidal activity than Hibitane champ with chlorhexidine concentrations 2 fold less. At 90, 50, 25, 10 and 5% concentrations, bactericidal activity (5 log10 reduction of the initial bacterial count) was effective in one minute. After 5 to 15 minutes, activity persisted at 1 and 0.5% concentrations. The 0.1% solution was inefficacious. This report disclosed an important security margin in antiseptic activity.
authors.affiliation: Laboratoire central de Microbiologie, Hôpital Edouard-Herriot, Lyon, France.
authors.forename: M E
authors.initials: ME
authors.lastname: Reverdy
authors.forename: M
authors.initials: M
authors.lastname: Rougier
authors.forename: J
authors.initials: J
authors.lastname: Fleurette
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000008:administration & dosage / Q000494:pharmacology; D001592:Benzyl Alcohols / Q000008:administration & dosage / Q000494:pharmacology; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D016983:Enterococcus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D066298:In Vitro Techniques; D009067:Mouthwashes; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D001592:Benzyl Alcohols; D004338:Drug Combinations; D009067:Mouthwashes; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
vernacular_title: Cinétique de bactéricidie in vitro de la biseptine antiseptique associant trois principes actifs.