title: Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from Aspergillus aculeatus.
abstract: A cDNA was isolated from an Aspergillus aculeatus cDNA library using synthetic oligodeoxyribonucleotide mixtures that corresponded to the internal amino acid (aa) sequence of mature beta-glucosidase 1 (BGL1). Analysis of the nucleotide sequence of the cloned cDNA insert revealed a 2580-bp open reading frame (ORF) that encoded a 860-aa protein. The deduced aa sequence of the ORF shared sequence similarity with several BGL from other microorganisms.
authors.affiliation: Department of Applied Biological Chemistry, University of Osaka Prefecture, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: T
authors.initials: T
authors.lastname: Enoki
authors.forename: S
authors.initials: S
authors.lastname: Tsurumaki
authors.forename: J
authors.initials: J
authors.lastname: Sumitani
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Ooi
authors.forename: M
authors.initials: M
authors.lastname: Arai
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004271:DNA, Fungal; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D001617:beta-Glucosidase