Cloning and sequencing of cDNA encoding 4-aminobenzoate hydroxylase from Agaricus bisporus.
A cDNA clone encoding 4-aminobenzoate hydroxylase (EC 1.14.13.27) has been isolated using a probe prepared by PCR on the basis of partially determined amino acid sequences of the enzyme. The cDNA contained 1380-base pair open reading frame encoding 460 amino acid residues (M(r) 50974), 14-base pair 5'-untranslated region and 123-base pair 3'-untranslated region including a poly(A) tail of 20 nucleotides. All of the partially determined amino acid sequences were shown to be included in the deduced amino acid sequence. Homology analyses showed that the two regions on the enzyme share other flavoproteins such as salicylate hydroxylase and p-hydroxybenzoate hydroxylase.
Tsuji
H
H
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Oka
T
T
Kimoto
M
M
Hong
Y M
YM
Natori
Y
Y
Ogawa
T
T
D000244:Adenosine Diphosphate / Q000378:metabolism; D000364:Agaricus / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D012331:RNA, Fungal / Q000235:genetics; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010446:Peptide Fragments; D012331:RNA, Fungal; D005182:Flavin-Adenine Dinucleotide; D000244:Adenosine Diphosphate; D006899:Mixed Function Oxygenases; C050092:4-aminobenzoate hydroxylase