title: Lymphocyte subpopulations and catecholamines; daytime variations and relationships.
abstract: The diurnal variations and their possible relations with endogenous catecholamines have been studied in 16 apparently healthy subjects (8 women and 8 men, between 22-35 years of age). A butterfly cannula was inserted into the flexure vein of the elbow at 8 h and at 20 h, a blood sample being taken after three 15-minute intervals. Leukocytes, lymphocytes, monocytes, and granulocytes were quantified in a cell counter while lymphocyte subpopulations were determined with flow cytometry in cells labelled with specific monoclonal antibodies. Plasma catecholamine concentrations were measured by high-pressure liquid chromatography. Significant differences for leukocyte circulating levels were found, as well as for all subpopulations measured at different times of day, save NK cells and monocytes. A statistically significant correlation was also found both between leukocytes, all subpopulations and epinephrine save B lymphocytes and NK cells, and between total lymphocytes, T lymphocytes, CD4, CD4/45RA+ subtypes and norepinephrine. In conclusion, diurnal rhythms were proved to exist in leukocyte and lymphocyte subpopulations; the significant correlation between these cells and catecholamine plasma levels suggests the presence of a possible mechanism that connects the cellular immunity with determined hormones.
authors.affiliation: C.S. Virgen de las Nieves, Servicio de Análisis Clínicos, Granada, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Infante
authors.forename: F
authors.initials: F
authors.lastname: Perán
authors.forename: M
authors.initials: M
authors.lastname: Martínez
authors.forename: R
authors.initials: R
authors.lastname: Poyatos
authors.forename: A
authors.initials: A
authors.lastname: Roldán
authors.forename: C
authors.initials: C
authors.lastname: Ruiz
authors.forename: F
authors.initials: F
authors.lastname: Garrido
mesh_terms: D000328:Adult; D002395:Catecholamines / Q000097:blood*; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008297:Male
chemical_list: D002395:Catecholamines