title: Mechanical strain-induced proliferation of osteoblastic cells parallels increased TGF-beta 1 mRNA.
abstract: It is well known that mechanical stimulation can prompt healing of bone fractures. However, the mechanism involved is less clear. In this study, we found that a 0.17% cyclic, biaxial mechanical strain delivered at 1 Hz increased proliferation of MC3T3-E1 cells, a clonal osteoblastic cell line. Mechanical strain also increased the level of TGF-beta 1 mRNA determined by quantitative reverse transcription/ polymerase chain reaction. Previous reports have shown that neomycin and W-7, which are inhibitors in the inositol phosphate/calmodulin pathway, blocked mechanical strain-induced proliferation of the osteoblast cells. Interestingly, we found that neomycin and W-7 can also block mechanical stimulation-induced elevation of TGF-beta 1 mRNA. Finally, using an antibody which blocked the action of TGF-beta 1, we found that the increased MC3T3-E1 cell proliferation induced by mechanical strain did not depend on the action of TGF-beta 1.
authors.affiliation: Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia 19082-6081, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zhuang
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: A D
authors.initials: AD
authors.lastname: Tahernia
authors.forename: C L
authors.initials: CL
authors.lastname: Levitz
authors.forename: W T
authors.initials: WT
authors.lastname: Luchetti
authors.forename: C T
authors.initials: CT
authors.lastname: Brighton
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002455:Cell Division / Q000235:genetics*; D007295:Inositol Phosphates / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D002147:Calmodulin; D007295:Inositol Phosphates; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta