Cerium stimulates protein biosynthesis in rat heart in vivo.
The aim of the study was to ascertain whether Ce, a lanthanide that has been implicated in the pathogenesis of tropical endomyocardial fibrosis, interferes with the biosynthetic repertoire of the cardiac muscle in vivo. Female Sprague-Dawley rats received Ce chloride iv at 1.3 mg/kg body wt.; controls received an equal volume of physiological saline. Rates of protein synthesis and transcription in cardiac muscle, measured in terms of incorporation of (3H)-phenylalanine and (3H)-uridine, respectively, into trichloroacetic acid-insoluble material were found to be significantly higher in Ce-treated animals. As low levels of Ce were earlier shown to stimulate collagen as well as noncollagen protein synthesis in cardiac fibroblasts in vitro, the stimulatory effect of the element in vivo reported here supports the speculation that it may influence the expression of proteins like collagen in the heart and contribute to their accumulation as in endomyocardial fibrosis.
Kumar
B P
BP
Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
D'Souza
S L
SL
Shivakumar
K
K
Rathinam
K
K
D000818:Animals; D002563:Cerium / Q000008:administration & dosage / Q000633:toxicity*; D004719:Endomyocardial Fibrosis / Q000209:etiology; D005260:Female; D006321:Heart / Q000187:drug effects*; D007275:Injections, Intravenous; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014238:Trichloroacetic Acid / Q000737:chemistry; D014316:Tritium / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D014316:Tritium; D002563:Cerium; D010649:Phenylalanine; D014238:Trichloroacetic Acid; D012313:RNA; D014529:Uridine