Apoptosis in HIV-1 Infection.
We have studied apoptosis in lymph node and peripheral blood mononuclear cells (PBMCs) from HIV-infected children and adults and SIV-infected rhesus macaques. In lymph nodes, we found that apoptosis and productive infection occurred only rarely in the same cell. There was, however, a direct correlation between the numbers of apoptotic and productively-infected cells. In HIV-infected children, we found a direct correlation between disease severity and percentage CD4+ T cell apoptosis (p = 0.001). Both CD4+ and CD8+ T cell apoptosis were directly related to CD4+ T cell depletion (p = 0.006 and p = 0.01, respectively). In addition, we found a trend towards diminished CD4+ T cell apoptosis on anti-retroviral agents. These findings suggest that apoptosis of uninfected cells may be important in HIV pathogenesis and that measurement of apoptosis may be a useful marker of disease activity.
Cotton
M F
MF
Pediatric Infectious Disease Section, Children's Hospital, University of Colorado Health Sciences Center, Denver 80218, USA.
Cassella
C
C
Rapaport
E L
EL
Tseng
P O
PO
Marschner
S
S
Finkel
T H
TH
D000328:Adult; D000818:Animals; D019380:Anti-HIV Agents / Q000627:therapeutic use; D017209:Apoptosis; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D018450:Disease Progression; D015658:HIV Infections / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D015497:HIV-1; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D015302:Simian Immunodeficiency Virus
D019380:Anti-HIV Agents; D015415:Biomarkers