[Identification of T cell epitopes by reverse immunogenetics].
Novel Strategy to identify CTL epitopes called "reverse immunogenetics" was introduced. CTL epitopes were identified as follows; (i) Identification of the motif of HLA class I binding peptides (ii) Selection of sequences matched to the motif of HLA class I binding peptides from HIV proteins and synthesis of peptides (iii) Identification of HLA class I binding peptides by the peptide binding assay (iv) Induction of CTL from PBL of HIV 1 infected individuals by HLA class I binding peptides. Multiple HIV-I epitopes presented by HLA-B35 were identified using this strategy.
Takiguchi
M
M
Department of Tumor Biology, University of Tokyo.
D000939:Epitopes / Q000302:isolation & purification*; D015497:HIV-1; D015797:HLA-B35 Antigen / Q000302:isolation & purification; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007125:Immunogenetics; D007158:Immunologic Techniques; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D015797:HLA-B35 Antigen; D015395:Histocompatibility Antigens Class I; D010455:Peptides