The effect of N-3 PUFA rich diet upon macrophage and lymphocyte metabolism and function.
A large body of evidence from experimental studies has documented that n-3 fatty acids can modify a variety of cell functions and disease states. As lymphocytes and macrophages are important cells for the development of the inflammatory and non-inflammatory immune response and are known to utilize high rates of glucose and glutamine, we have evaluated the effect of n-3 PUFA rich diet on the metabolisation of glucose and glutamine these cells, as well as the effect of one such diet upon the proliferative response of lymphocytes and the phagocytic capacity and hydrogen peroxide production by macrophages. The diet provoked an increase in the flux of glucose through the Krebs cycle in macrophages as well as a reduction in G6PDh and glutaminase activity in these cells. Lymphocytes from n-3 PUFA rich diet-fed rats showed a reduction in glucose and glutamine decarboxylation. Taken together the data show that, at least in part, the functional changes observed in macrophages and lymphocytes from n-3 PUFA-rich diet fed rats are related to the effect of this diet upon glucose and glutamine metabolism, leading to immunosuppression.
Costa Rosa
L F
LF
Dept. Histology and Embriology, University of São Paulo, Brazil. costarosa@bmb.icb1.usp.br
Safi
D A
DA
Guimarães
A R
AR
D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D005973:Glutamine; D019344:Lactic Acid; D006861:Hydrogen Peroxide; D005947:Glucose