Enhanced polymer one-step staining for proliferating cell nuclear antigen in squamous cell carcinoma of the tongue.
A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopathology and in performing double immunostaining.
Sano
K
K
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Japan.
Sekine
J
J
Inokuchi
T
T
Pe
M B
MB
Ma
G
G
D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D011108:Polymers; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*; D014059:Tongue / Q000276:immunology / Q000473:pathology; D014062:Tongue Neoplasms / Q000276:immunology* / Q000473:pathology
D011108:Polymers; D018809:Proliferating Cell Nuclear Antigen