DNA-aneuploidy as marker for neoplasia in G1-urothelial carcinomas.
Biopsies from 60 grade-1 urothelial carcinomas and 50 normal bladder mucosae have been investigated by DNA-image-cytometry after enzymatic cell separation to determine the diagnostic sensitivity of DNA-aneuploidy for the identification of low grade urothelial neoplasia. Two different modes to detect DNA-aneuploidy were compared: the conventional application of a threshold at 2.2c for the modal DNA value and the use of the Kolmogoroff-Smirnow (KS) test to compare GO/1 phase fractions of the cells under analysis and the internal reference cells. Whereas the specificity for both types of analysis was 100%, the sentitivity of the latter method was 73.3% compared to 48.3% of the conventional procedure. Therefore, the application of the KS-test for the identification of DNA- stemlines as aneuploid results in an increased detection rate was compared with the conventional threshold procedure. The high prevalence of DNA- stemline aneuploidy even in low grade urothelial cancers may qualify this marker as a possible aid in the cytologic evaluation of urine and bladder washings.
Planz
B
B
Department of Urology, Technical University of Aachen, Germany.
Striepecke
E
E
Wolff
J M
JM
Effert
P
P
Jakse
G
G
Böcking
A
A
D000782:Aneuploidy; D014408:Biomarkers, Tumor / Q000235:genetics*; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D019044:Image Cytometry; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers