Constitutive steroidogenesis does not require the actions of cAMP on cholesteryl ester hydrolysis or internalization of plasma membrane cholesterol.
The R2C Leydig tumor cells demonstrate constitutive steroidogenesis as well as the constitutive production of the steroidogenic acute regulatory peptide (StAR). Since the introduction of StAR into cells does not by itself reproduce the steroidogenic response of cAMP, the present studies were designed to determine whether processes normally stimulated by cAMP in responsive cells were maximally, constitutively expressed in the R2C cells. Measurements of cholesteryl ester hydrolytic rate and plasma membrane cholesterol internalization revealed that both processes are stimulated by dibutyryl-cAMP in R2C cells. The effect of cAMP on these processes was of the same magnitude as that detected in hormonally responsive cells. Thus, in R2C cells the unstimulated basal rate of either process was sufficient to maintain maximal stimulation of steroidogenesis.
Freeman
D A
DA
Department of Internal Medicine and Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
D003994:Bucladesine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D006868:Hydrolysis; D007984:Leydig Cell Tumor / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D000098682:Steroidogenic Acute Regulatory Protein
D002788:Cholesterol Esters; D010750:Phosphoproteins; D013256:Steroids; D000098682:Steroidogenic Acute Regulatory Protein; D003994:Bucladesine; D002784:Cholesterol; D000242:Cyclic AMP