title: Effect of bone wax extract on the frequency of bone marrow erythrocyte micronuclei in mice.
abstract: The present study evaluated the induction of micronuclear potency of indigenously developed bone wax saline extract. The experiment was designed with 2 batches, each consisting of 8 groups with 3 male Swiss albino mice each. In each batch, the first 3 groups received Chitra's bone wax saline extract and the second 3 groups received Johnson & Johnson bone wax saline extract at 12.5, 25.0 or 50.0 ml/kg body weight ip for 2 d. The remaining 2 groups were vehicle (saline) and positive (cyclophosphamide) controls. Animals were sacrificed 24 and 36 h after the second dosing and bone marrow smears were prepared and evaluated for micronuclei. The Chitra's and Johnson & Johnson's bone wax extracts failed to induce micronuclei in bone marrow erythrocytes of mice.
authors.affiliation: Toxicology Group, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
authors.forename: K
authors.initials: K
authors.lastname: Rathinam
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage / Q000633:toxicity; D014885:Waxes / Q000633:toxicity*
chemical_list: D001672:Biocompatible Materials; D012462:Saline Solution, Hypertonic; D014885:Waxes; D003520:Cyclophosphamide