High efficiency photolabeling of human serum albumin and human gamma-globulin with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate.
The efficiency of photolabeling of HSA and IgG with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate has been studied using size exclusion chromatography in conjunction with liquid scintillation counting. Labeling efficiencies of 78% for HSA and 82% for IgG have been determined. The extent of bond insertion into proteins exceeds the C-H insertion efficiency in cyclohexane with less wastage into anilinium and azo side products. These results suggest that the photoprobe accesses hydrophobic regions of both proteins prior to photolysis.
Pandurangi
R S
RS
Department of Chemistry, H.S. Truman Memorial VA Hospital, University of Missouri, Columbia 65211, USA.
Karra
S R
SR
Kuntz
R R
RR
Volkert
W A
WA
D000345:Affinity Labels / Q000138:chemical synthesis / Q000737:chemistry*; D001565:Benzoates / Q000138:chemical synthesis / Q000737:chemistry; D002850:Chromatography, Gel; D003510:Cyclohexanes; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D010777:Photochemistry; D010782:Photolysis; D012588:Scintillation Counting; D012709:Serum Albumin / Q000737:chemistry*; D005719:gamma-Globulins / Q000737:chemistry*
D000345:Affinity Labels; D001565:Benzoates; D003510:Cyclohexanes; D007074:Immunoglobulin G; D012709:Serum Albumin; D005719:gamma-Globulins; C098120:methyl 4-azido-2,3,5,6-tetrafluorobenzoate