title: Effect of paclitaxel alone or in combination on the intracellular penetration and activity of quinolones in human neutrophils.
abstract: We evaluated the effect of paclitaxel alone or in combination with cisplatin or doxorubicin, on the intracellular penetration and activity of ciprofloxacin, ofloxacin, levofloxacin and sparfloxacin in human polymorphonuclear leukocytes (PMN). In general, paclitaxel either alone or in combination did not affect the intracellular concentrations of the quinolones evaluated (intracellular to extracellular concentration ratio (C/E > 4). However, at high concentrations (20 mg/L) paclitaxel decreased the penetration of ofloxacin and levofloxacin although the C/E ratios remained higher than 3. Paclitaxel alone or in combination affected neither the production of oxygen radicals by PMN nor the intracellular activity of the quinolones against Staphylococcus aureus.
authors.affiliation: Department of Microbiology, School of Medicne, Seville, Spain.
authors.forename: I
authors.initials: I
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Pascual
authors.forename: J
authors.initials: J
authors.lastname: Salvador
authors.forename: M C
authors.initials: MC
authors.lastname: Conejo
authors.forename: E J
authors.initials: EJ
authors.lastname: Perea
mesh_terms: D000890:Anti-Infective Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000493:pharmacokinetics / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D024841:Fluoroquinolones; D006801:Humans; D064704:Levofloxacin; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D015242:Ofloxacin / Q000493:pharmacokinetics / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology*; D015363:Quinolones / Q000493:pharmacokinetics / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D024841:Fluoroquinolones; D015363:Quinolones; D002939:Ciprofloxacin; D064704:Levofloxacin; D004317:Doxorubicin; D015242:Ofloxacin; D017239:Paclitaxel; D002945:Cisplatin; C061363:sparfloxacin