RS cyclophilins: identification of an NK-TR1-related cyclophilin.
We report the isolation of a large cyclophilin protein containing RS (arginine-serine) repeats from a yeast two-hybrid screen using ClK (CDC28/cdc2-like kinase) as a probe. This Clk associating RS-cyclophilin (CARS-Cyp) possesses 39% homology to the NK-TR1 (natural killer tumor recognition protein-1) we have previously characterized (Anderson et al. (1993) Proc. Natl. Acad. Sci. USA 90 (1993) 542-546). CARS-Cyp is expressed in a variety of tissues and cell types, and codes for a protein with a predicted mass of 89 kDa containing a cyclophilin-related domain, two Nopp140 (nucleolar phosphoprotein of 140 kDa)-related domains, and a large RS domain. The RS-cyclophilins, a novel class of proteins, may play an important role in the regulation of pre-mRNA splicing.
Nestel
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FP
Laboratory of Experimental Immunology, DBS, NCI-FCRDC, Frederick, MD, USA.
Colwill
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K
Harper
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S
Pawson
T
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Anderson
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SK
D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D021983:Cyclophilins; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012326:RNA Splicing; D055607:Receptors, Natural Killer Cell; D012694:Serine / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry
D001798:Blood Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D055607:Receptors, Natural Killer Cell; D012694:Serine; D001120:Arginine; C066780:Clk dual-specificity kinases; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D000591:Amino Acid Isomerases; D021983:Cyclophilins; C102923:PPIG protein, human