title: Renal sensory receptor activation causes prostaglandin-dependent release of substance P.
abstract: Renal mechanoreceptor (MR) activation by increased ureteral pressure (increases UP) results in an increase in afferent renal nerve activity (ARNA) that is blocked by substance P receptor blockade and prostaglandin (PG) synthesis inhibition. To examine the interaction between substance P and PGs, the release of substance P and PGE into the renal pelvis was studied before and during renal pelvic perfusion with indomethacin. Before indomethacin, increases UP increased ARNA 43 +/- 6% and renal pelvic release of substance P from 11 +/- 3 to 29 +/- 8 pg/min and PGE from 319 +/- 71 to 880 +/- 146 pg/min. Indomethacin blocked the increases in ARNA and release of substance P and PGE produced by increases UP. Time control experiments showed reproducible increases in ARNA and release of substance P and PGE during increases UP. Mechanical stimulation of the renal pelvic wall in vitro resulted in an increase in PGE release from 110 +/- 8 to 722 +/- 152 pg/min, which was abolished by indomethacin, suggesting a de novo PGE synthesis. The data suggest that increases UP results in a renal pelvic release of PGE, which facilitates the release of substance P and activation of renal pelvic MR.
authors.affiliation: Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, USA.
authors.forename: U C
authors.initials: UC
authors.lastname: Kopp
authors.forename: D M
authors.initials: DM
authors.lastname: Farley
authors.forename: L A
authors.initials: LA
authors.lastname: Smith
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000294:innervation*; D007682:Kidney Pelvis / Q000378:metabolism; D008297:Male; D009424:Nervous System Physiological Phenomena; D010812:Physical Stimulation; D011312:Pressure; D011453:Prostaglandins / Q000502:physiology*; D011458:Prostaglandins E / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000502:physiology*; D013373:Substance P / Q000378:metabolism*; D014513:Ureter / Q000502:physiology
chemical_list: D011453:Prostaglandins; D011458:Prostaglandins E; D013373:Substance P; D007213:Indomethacin