title: Preparation of specific antiserum to 17 alpha-estradiol 17-N-acetylglucosaminide.
abstract: A specific enzyme immunoassay (EIA) method has been developed for 17 alpha-estradiol 17-N-acetylglucosaminide as a model compound instead of 15 alpha-hydroxyestrogen 15-N-acetylglucosaminides. Two new haptens, 3-(omega-carboxyalkyl) ether derivatives of 17-alpha-estradiol 17-N-acetylglucosaminide, were synthesized and conjugated with bovine serum albumin (BSA). The EIA was newly established using specific antiserum elicited against 3-(1-carboxypropyl) ether of 17 alpha-estradiol 17-N-acetylglucosaminide (17NAG CPE)-BSA conjugate and beta-galactosidase-labeled 17NAG CPE as a labeled antigen. An appropriate dose-response curve of EIA for 17 alpha-estradiol 17-N-acetylglucosaminide was obtained in the range of 20-1000 pg/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related compounds including 15 alpha-N-acetylglucosaminides. The proposed method will be applicable to the preparation of antisera for use in EIA of 15 alpha-hydroxyestrogen 15-N-acetylglucosaminides.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Nakagomi
authors.forename: S
authors.initials: S
authors.lastname: Tsuruta
authors.forename: C
authors.initials: C
authors.lastname: Yamawaki
authors.forename: J
authors.initials: J
authors.lastname: Goto
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuki
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis* / Q000276:immunology; D000818:Animals; D004958:Estradiol / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012710:Serum Albumin, Bovine
chemical_list: D007106:Immune Sera; D012710:Serum Albumin, Bovine; D004958:Estradiol; D000117:Acetylglucosamine