Translation attenuation regulation of chloramphenicol resistance in bacteria--a review.
The chloramphenicol (Cm)-inducible cat and cmlA genes are regulated by translation attenuation, a regulatory device that modulates mRNA translation. In this form of gene regulation, translation of the CmR coding sequence is prevented by mRNA secondary structure that sequesters its ribosome-binding site (RBS). A translated leader of nine codons precedes the secondary structure, and induction results when a ribosome becomes stalled at a specific site in the leader. Here we demonstrate that the site of ribosome stalling in the leader is selected by a cis effect of the nascent leader peptide on its translating ribosome.
Lovett
P S
PS
Department of Biological Sciences, University of Maryland, Baltimore County, Catonsville, MD 21228, USA. lovett@umbc.edu
D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D002702:Chloramphenicol Resistance / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics; D008957:Models, Genetic; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors; D014176:Protein Biosynthesis; D012338:RNA, Ribosomal, 23S / Q000378:metabolism
D012338:RNA, Ribosomal, 23S; D015500:Chloramphenicol O-Acetyltransferase; D010458:Peptidyl Transferases