[Molecular approach to detection of micrometastatic prostatic cancer cells in the lymph nodes and the bone marrow].
We detected micrometastatic prostate cancer cells in lymph nodes and bone marrow using reverse transcriptase-polymerase chain reaction (RT-PCT) specific for prostate-specific antigen (PSA). RT-PCR revealed PSA mRNA in two lymph nodes obtained from two patients with negative histological and immunohistochemical analyses for lymph node metastases. Of 26 patients with negative bone scan imaging, 7 had PSA mRNA detected in the bone marrow by RT-PCR. The RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node and bone metastases in patients with prostate cancer.
Deguchi
T
T
Department of Urology, Gifu University School of Medicine.
Yang
M
M
Nishino
Y
Y
Ehara
H
H
Takahashi
Y
Y
Kawata
Y
Y
Ito
S
S
Ito
Y
Y
Doi
T
T
D019046:Bone Marrow Neoplasms / Q000556:secondary*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000235:genetics; D011471:Prostatic Neoplasms / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity
D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D017430:Prostate-Specific Antigen