Selective uptake of boronated low-density lipoprotein in melanoma xenografts achieved by diet supplementation.
The lipid core of human plasma low-density lipoprotein (LDL) was extracted using hexane and the LDL reconstituted with the addition of n-octyl-carborane. Biodistribution studies of the boronated LDL were performed in BALB/c mice bearing subcutaneous Harding-Passey melanoma xenografts. When diet supplementation with coconut oil and cholesterol for 21 days and regular dosing with hydrocortisone for 7 days before the studies was used to down-regulate the liver LDL receptors and the adrenal receptors, respectively, the tumour-blood boron concentration ratio of 5:1 was achieved.
Setiawan
Y
Y
Department of Pharmacy, University of Sydney, NSW, Australia.
Moore
D E
DE
Allen
B J
BJ
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D001895:Boron / Q000032:analysis / Q000493:pharmacokinetics*; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000378:metabolism; D000074263:Coconut Oil; D015536:Down-Regulation; D004337:Drug Carriers; D006207:Half-Life; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D010938:Plant Oils / Q000493:pharmacokinetics; D011973:Receptors, LDL / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D002791:Cholesterol, Dietary; D004337:Drug Carriers; D008077:Lipoproteins, LDL; D010938:Plant Oils; D011973:Receptors, LDL; D001895:Boron; D000074263:Coconut Oil