title: A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution.
abstract: We have isolated cDNA clones encoding dihydropyrimidinase (DHPase) from human liver and its three homologues from human fetal brain. The deduced amino acid (aa) sequence of human DHPase showed 90% identity with that of rat DHPase, and the three homologues showed 57-59% aa identity with human DHPase, and 74-77% aa identity with each other. We tentatively termed these homologues human DHPase related protein (DRP)-1, DRP-2 and DRP-3. Human DRP-2 showed 98% aa identity with chicken CRMP-62 (collapsin response mediator protein of relative molecular mass of 62 kDa) which is involved in neuronal growth cone collapse. Human DRP-3 showed 94-100% aa identity with two partial peptide sequences of rat TOAD-64 (turned on after division, 64 kDa) which is specifically expressed in postmitotic neurons. Human DHPase and DRPs showed a lower degree of aa sequence identity with Bacillus stearothermophilus hydantoinase (39-42%) and Caenorhabditis elegans unc-33 (32-34%). Thus we describe a novel gene family which displays differential tissue distribution: i.e., human DHPase, in liver and kidney; human DRP-1, in brain; human DRP-2, ubiquitously expressed except for liver; human DRP-3, mainly in heart and skeletal muscle.
authors.affiliation: Department of Pediatrics, Nagoya City University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hamajima
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Sakata
authors.forename: N
authors.initials: N
authors.lastname: Tamaki
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Nonaka
mesh_terms: D000581:Amidohydrolases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002645:Chickens; D002874:Chromosome Mapping; D018076:DNA, Complementary; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009124:Muscle Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D000581:Amidohydrolases; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C102924:CRMP1 protein, human; D000098855:Collapsin Response Mediator Protein 1; C102926:DPYSL3 protein, human; C095114:collapsin response mediator protein-2; C020047:dihydropyrimidinase