Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei.
Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.
Stenos
J
J
Victorian Infectious Diseases Reference Laboratory, Fairfield Hospital, Victoria, Australia.
Ross
B
B
Feng
H M
HM
Crocquet-Valdes
P
P
Walker
D
D
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D004269:DNA, Bacterial; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial