Purification of autocrine growth factor from conditioned medium of rat sarcoma (XC) cells.
Transformation of rat cells by Rous sarcoma virus(es) induced the release of growth factors into serum-free conditioned media. An PR-RSV-transformed rat cell line, XC, produced and released polypeptide factors which promote anchorage-dependent and anchorage-independent growth of XC cells. One of the autocrine factors of XC cells was purified to homogeneity by four-step procedure: ultrafiltration, ion-exchange chromatography on MonoS, reverse-phase chromatography on Spherisorb ODS2 and gel filtration on Superose 12. The factor gave a single band on SDS-electrophoresis on polyacrylamide gel and was assumed to have a molecular weight of 16 kDa. The factor is a potent mitogen for XC cells; half-maximal stimulation of DNA synthesis was achieved at a concentration of 0.8 ng/ml. The peptide is probably one of the family of EGF-like heparin-binding growth factors.
Checiówna
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Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Klein
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D000818:Animals; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral / Q000502:physiology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D017077:Culture Media, Conditioned / Q000032:analysis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000096:biosynthesis / Q000302:isolation & purification*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism*; D014462:Ultrafiltration
D017077:Culture Media, Conditioned; D004273:DNA, Neoplasm; D006133:Growth Substances; D009363:Neoplasm Proteins