Involvement of gicerin, a cell adhesion molecule, in tracheal development and regeneration.
Gicerin is a novel cell adhesion protein that belongs to the immunoglobulin superfamily. Gicerin protein adheres to neurite outgrowth factor, an extracellular matrix protein in the laminin family, and also exhibits homophilic adhesion. In the present study, we investigated the involvement of gicerin and neurite outgrowth factor in tracheal development and regeneration. In an early embryonic stage, gicerin protein was highly expressed in tracheal epithelial cells, but not in loosely arranged mesenchymal cells. During development, mesenchymal cells become condensed around the tracheal epithelium and then differentiate into muscle and cartilage; high levels of gicerin expression were observed in these cells. In the later embryonic and posthatching stages, no gicerin expression was detected in tracheal epithelium or cartilage. In addition, expression of gicerin increased transiently in the tracheal epithelium during the regeneration after tracheitis induced by the infectious bronchitis virus. Furthermore, a polyclonal antibody against gicerin inhibited the epithelial regeneration in tracheal organ cultures. These findings suggest that glcerin plays an important role in both tracheal development and regeneration.
Tsukamoto
Y
Y
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
Taira
E
E
Kotani
T
T
Yamate
J
J
Wada
S
S
Takaha
N
N
Miki
N
N
Sakuma
S
S
D000818:Animals; D000906:Antibodies; D030161:Avian Proteins; D015153:Blotting, Western; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D002642:Chick Embryo; D018352:Coronavirus Infections; D004848:Epithelium / Q000737:chemistry / Q000502:physiology / Q000821:virology; D007150:Immunohistochemistry; D001351:Infectious bronchitis virus; D008024:Ligands; D024101:Mitochondrial Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D012038:Regeneration / Q000502:physiology; D012269:Ribosomal Proteins; D014132:Trachea / Q000166:cytology* / Q000196:embryology / Q000821:virology; D014136:Tracheitis / Q000503:physiopathology* / Q000821:virology
D000906:Antibodies; D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D008024:Ligands; C100930:MRPL49 protein, human; D024101:Mitochondrial Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C086935:gicerin protein, Gallus gallus