title: Enzyme-linked immunosorbent assay for human MRP-8/MRP-14 proteins and their quantitation in plasma of hematological patients.
abstract: We developed a quantitative enzyme immunoassay for human MRP-8/MRP-14, neutrophil cytosolic proteins with calcium-binding and bacteriastatic properties. MRP-8/MRP-14 concentrations were measured in the plasma of 23 healthy volunteers and in 75 patients with hematological disorders. These proteins were detected in plasma of healthy blood donors with mean +/- standard deviation 167 +/- 114 ng/ml. MRP-8/MRP-14 plasma levels ranged from 435 to 13280 ng/ml in patients with chronic myeloid leukemia (CML), from 50 to 7570 ng/ml in chronic lymphoid leukemia (CLL), from 450 to 2790 ng/ml in polycythemia vera (PV), and were significantly higher than in healthy blood donors (P < 0.01 for CML and P < 0.05 for others). In CML patients MRP-8/MRP-14 levels strongly correlated with total blood WBC count (r = 0.82) and with neutrophilic granulocyte (NG) count (r = 0.80). Correlation of these values in PV amounted to r = 0.70 and r = 0.46, respectively. In CLL patients MRP-8/MRP-14 levels strongly correlated with total WBC count (r = 0.92), but not with the NG count. We suggest that MRP-8/MRP-14 quantitation may serve as a marker of neutrophil pool turnover in some hematological disorders.
authors.affiliation: Institue of Hematology and Blood Transfusion, Minsk, Belarus.
authors.forename: G E
authors.initials: GE
authors.lastname: Ivanov
authors.forename: N I
authors.initials: NI
authors.lastname: Misuno
authors.forename: V E
authors.initials: VE
authors.lastname: Ivanov
mesh_terms: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000276:immunology; D040501:Calgranulin A; D040502:Calgranulin B; D004797:Enzyme-Linked Immunosorbent Assay; D006402:Hematologic Diseases / Q000097:blood* / Q000276:immunology*; D006801:Humans; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B