Flow cytometric DNA analysis of gastric cancer that is invading the muscularis propria.
OBJECTIVE
To find out if there is any correlation between the DNA aneuploidy in gastric cancer that is invading the muscularis propria and survival.


METHODS
Retrospective study.


METHODS
Teaching hospital, Japan.


METHODS
52 patients who underwent gastric resection for gastric cancer that was invading the muscularis propria, and for whom clinical follow-up data were available.


METHODS
Flow cytometric measurement of DNA in paraffin-embedded tissue.


METHODS
Correlation between survival and clinicopathological variables including ploidy.


RESULTS
22 (42%) of the cases were aneuploid and 30 (58%) were diploid. The lymph node status and DNA ploidy were the significant prognostic variables, but a multivariate analysis showed that only DNA ploidy was significant.


CONCLUSIONS
Nuclear DNA ploidy is an independent prognostic factor in gastric cancer that is invading the muscularis propria.
Mizunuma
H
H
Department of Surgery II, Fukushima Medical College, Japan.
Tsuchiya
A
A
Ando
Y
Y
Kikuchi
Y
Y
Abe
R
R
D000782:Aneuploidy; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D016612:Paraffin Embedding; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate
D004273:DNA, Neoplasm