Acute increase by portal insulin in intestinal glucose absorption via hepatoenteral nerves in the rat.
OBJECTIVE
Insulin exerts a strict short-term control of glucose disappearance by glucose storage as well as degradation in the liver and peripheral insulin target tissues, but an acute control of glucose appearance by glucose absorption in the intestine is as yet unknown. The aim of the present study was to evaluate, whether insulin acutely modulates intestinal glucose absorption.


METHODS
In the isolated, nonrecirculating joint perfusion of the small bowel and liver of the rat via the celiac trunc and the superior mesenteric artery, glucose absorption was examined without and with infusion of insulin via the portal vein.


RESULTS
Portal insulin enhanced acutely intestinal glucose absorption. This thus far unknown stimulatory effect of portal insulin was dose-dependent and detectable at physiological insulin concentrations. Atropine infused into the superior mesenteric artery completely prevented the insulin-dependent increase in intestinal glucose absorption, and carbachol caused a similar increase as portal insulin.


CONCLUSIONS
Portal insulin dose-dependently generated a signal in the liver or portal vein. This signal was transmitted in a retrograde direction against the blood stream in the portal vein to the small intestine via hepatoenteral muscarinic nerves. This signal markedly increased intestinal glucose absorption.
Stümpel
F
F
Institute of Biochemistry and Molecular Cell Biology, Georg-August University, Göttingen, Germany.
Kucera
T
T
Gardemann
A
A
Jungermann
K
K
D000818:Animals; D005947:Glucose / Q000493:pharmacokinetics*; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology; D007408:Intestinal Absorption; D007668:Kidney / Q000294:innervation*; D008099:Liver / Q000294:innervation*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009116:Muscarine / Q000378:metabolism; D009424:Nervous System Physiological Phenomena; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D051269:Sodium-Glucose Transporter 1
D007328:Insulin; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D051269:Sodium-Glucose Transporter 1; D009116:Muscarine; D005947:Glucose