title: Analysis of ifosforamide mustard, the active metabolite of ifosfamide, in plasma.
abstract: Ifosforamide mustard is the active metabolite of ifosfamide, a cytostatic drug. In this study a sensitive and selective method for the analysis of ifosforamide mustard in plasma is described. The method consists of direct derivatisation of ifosforamide mustard in plasma with diethyldithiocarbamate and subsequent solid-phase extraction of the resulting derivative. The analysis of the derivatisation product was performed by high-performance liquid chromatography with UV detection. The calibration graph was linear in the concentration range 0.45-45 microM and the minimum detectable concentration was 0.45 mumol. The samples were stabilised by addition of semicarbazide and sodium chloride. A patient's plasma sample was analysed by means of the described method. The ifosforamide mustard concentration was 2.3 microM.
authors.affiliation: RL-CERM, Zône Industrielle La Varenne, Riom, France.
authors.forename: G P
authors.initials: GP
authors.lastname: Kaijser
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
authors.forename: E
authors.initials: E
authors.lastname: Rozendom
authors.forename: A
authors.initials: A
authors.lastname: Bult
authors.forename: W J
authors.initials: WJ
authors.lastname: Underberg
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007069:Ifosfamide / Q000493:pharmacokinetics*; D010752:Phosphoramide Mustards / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010752:Phosphoramide Mustards; C027061:isophosphamide mustard; D007069:Ifosfamide