Isolation of a developmentally-regulated expressed sequence tag from bladder tissue using the mRNA differential display.
In order to gain insight into the molecular and cellular events that govern the structural and the functional properties in developing organs, we have conducted a study to identify genes that have a temporally-restricted expression in the bladder wall during fetal development. We utilized the mRNA differential display technique and compared the pattern of gene expression during the first, the second and the third trimester of gestation. We cloned and sequenced a cDNA fragment (bld-10) which was expressed during the second and third trimester but consistently absent during the first trimester. The bld-10 sequence is not related to any known gene in the GenBank database but has significant homology (89%) with human expressed sequence tag (EST) that has been cloned from human fetal heart and brain libraries. When used in Northern-blot hybridization as a probe, the fragment bld-10 generates two hybridization signals of 3.1 and 4.0 kb, that are minimally expressed during the first trimester of gestation and upregulated in the second and third trimester. Differential expression of this gene may be responsible for some of the profound changes which occur during organ development.
Chaqour
B
B
University of Pennsylvania, School of Dental Medicine, Department of Anatomy & Histology, Philadelphia 19104, USA.
Howard
P S
PS
Macarak
E J
EJ
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D001743:Urinary Bladder / Q000196:embryology* / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger