title: Immunocharacterization of Taenia solium oncosphere and metacestode antigens.
abstract: A partial immunocharacterization of oncosphere and metacestode antigens of Taenia solium was carried out and compared to antigens from other taeniid species. The results indicated that T. solium metacestode antigen contained epitopes cross reactive with rabbit anti-sera to adult and oncospheral stages of the parasite. Oncospheres, however, consisted largely of stage specific antigens. Western blot analysis indicated that T. solium and T. pisiformis shared several oncospheral antigens; however, this was not the case with T. solium and T. hydatigena. Western blot analysis showed a time-related increase in the number of molecules recognized by antibodies to T. solium oncosphere and metacestode antigens in pigs experimentally infected with T. solium eggs. Oncosphere specific antibodies were detected in pig sera one month after experimental infection whereas antibodies to cystic stage antigens were not present until the 3rd to 5th month post infection. Sera from neurocysticercotic patients as well as naturally infected cysticercotic pigs recognized high molecular weight antigens in the oncospheres.
authors.affiliation: Department of Parasitology, Liverpool School of Tropical Medicine, UK.
authors.forename: C
authors.initials: C
authors.lastname: Garcia-Allan
authors.forename: N
authors.initials: N
authors.lastname: Martínez
authors.forename: A
authors.initials: A
authors.lastname: Flisser
authors.forename: A
authors.initials: A
authors.lastname: Aluja
authors.forename: J C
authors.initials: JC
authors.lastname: Allan
authors.forename: P S
authors.initials: PS
authors.lastname: Craig
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D003551:Cysticercosis / Q000276:immunology; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D013552:Swine; D013621:Taenia / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D007106:Immune Sera