Isolation and sequence determination of cDNA encoding mouse rab 4 and candidate approach for the beige mutation in mice.
The Chediak-Higashi syndrome is characterized by partial albinism and recurrent infections with giant granules in granulocytes. This syndrome has been proposed to have a defect in vesicular transport. Rab 4 is a member of a family of Ras-related small GTP-binding proteins, which has been mapped in the locus of the Chediak-Higashi syndrome. We isolated a full length cDNA of rab 4 from a cDNA library of mouse liver. The clone is 1428 base pairs (bp) in length and contains a 639 bp open reading frame encoding a polypeptide of 213 residues. The deduced amino acid sequence is highly homologous to rab 4 from rat and human. We analyzed rab 4 as a candidate gene of the beige mouse, but we could not find any change in the sequence of the coding region of rab 4 mRNA.
Ikeda
H
H
Department of Pediatrics, Yamagata University School of Medicine, Japan.
Ikegami
T
T
Mitsui
T
T
Sendo
D
D
Hayasaka
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D051381:Rats; D020695:rab4 GTP-Binding Proteins
D018076:DNA, Complementary; D019204:GTP-Binding Proteins; D020695:rab4 GTP-Binding Proteins