title: Thiamine pyrophosphate effect and normalized erythrocyte transketolase activity ratio in Wernicke-Korsakoff patients and acute alcoholics undergoing detoxification.
abstract: Thiamine deficiency may be assessed clinically by an abnormally low specific erythrocyte transketolase activity and/or by abnormally large activation by thiamine diphosphate in vitro (or 'TPP effect'). In the present investigation, we report erythrocyte transketolase activation by TPP in acute alcoholics and Wernicke-Korsakoff patients undergoing detoxification. A new age-dependent parameter was used to improve the reliability of transketolase activity as an indicator of marginal thiamine deficiency. Thus normalized transketolase activity ratio (NTKZ), primary activation ratio (PAR) and further activation ratio (FAR) were measured in 29 acute alcoholics and 12 Wernicke-Korsakoff patients upon admission, and also on 47 control subjects. It was possible to follow up 14 of the 29 acute alcoholics after 7 days of treatment. Twenty-one per cent of the acute alcoholics and 33% of the Wernicke-Korsakoff patients, on admission to the detoxification Unit, had NTKZ values beyond the defined critical conditions for thiamine deficiency, whereas 7% of the former and 25% of the latter had PAR values beyond these critical conditions. Furthermore, all three parameters were significantly different in the Wernicke-Korsakoff patients compared to the other groups. The pattern of improvement of the different parameters on follow-up varied considerably and is difficult to explain, as only the NTKZ was statistically significant. Hence, only eight out of 14 acute alcoholics showed improvement in NTKZ, seven showed improvement of PAR and six showed improvement of FAR after treatment. Five patients showed improvement of both NTKZ and PAR and none of the patients showed improvement of all three parameters. We conclude that our findings confirm previous reports and that this modified transketolase activation test improves its reliability as an indicator of marginal thiamine deficiency.
authors.affiliation: Department of Neuropathology, Institute of Psychiatry, London, UK.
authors.forename: H K
authors.initials: HK
authors.lastname: Rooprai
authors.forename: O E
authors.initials: OE
authors.lastname: Pratt
authors.forename: G K
authors.initials: GK
authors.lastname: Shaw
authors.forename: A D
authors.initials: AD
authors.lastname: Thomson
mesh_terms: D000328:Adult; D000368:Aged; D000425:Alcohol Amnestic Disorder / Q000175:diagnosis / Q000201:enzymology / Q000534:rehabilitation*; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013832:Thiamine Deficiency / Q000175:diagnosis* / Q000201:enzymology / Q000534:rehabilitation; D013835:Thiamine Pyrophosphate; D014174:Transketolase / Q000097:blood*; D014899:Wernicke Encephalopathy / Q000175:diagnosis / Q000201:enzymology / Q000534:rehabilitation*
chemical_list: D014174:Transketolase; D013835:Thiamine Pyrophosphate