RNA phage Q beta coat protein as a carrier for foreign epitopes.
The Q beta gene C has been proposed as a new carrier for the exposure of foreign peptide sequences. Contrary to well-known 'display vectors' on the basis of coat proteins of RNA phage group I, group III phage Q beta-based vectors suggested application of the 195-amino acid extension of coat protein (CP) within the so-called A1 protein for insertion of the appropriate immunological epitopes. 'Mosaic' capsids presenting model hepatitis B virus preS1 and HIV-1 gp120 epitopes and formed by Q beta CP together with A1-derived proteins were obtained as a result of (1) suppression of leaky UGA stop codon of the CP gene and (2) simultaneous expression of 'pure' CP and full-length A1-derived genes obtained after the changing of CP-terminating UGA to strong UAA stop codon or sense GGA codon, respectively.
Kozlovska
T M
TM
Biomedical Research and Study Centre, University of Latvia, Riga, Latvia.
Cielens
I
I
Vasiljeva
I
I
Strelnikova
A
A
Kazaks
A
A
Dislers
A
A
Dreilina
D
D
Ose
V
V
Gusars
I
I
Pumpens
P
P
D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics*; D018388:Codon, Terminator; D000939:Epitopes / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010454:Peptide Termination Factors; D011498:Protein Precursors / Q000235:genetics; D012317:RNA Phages / Q000235:genetics*
D018388:Codon, Terminator; D000939:Epitopes; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D006514:Hepatitis B Surface Antigens; D010446:Peptide Fragments; D010454:Peptide Termination Factors; D011498:Protein Precursors; C087971:peptide-chain-release factor 3; C050806:presurface protein 1, hepatitis B surface antigen