Binding and relaxation behaviour of prodan and patman in phospholipid vesicles: a fluorescence and 1H NMR study.
The relative location, binding behaviour and the solvent relaxation behaviour of the polarity sensitive membrane probes 6-propionyl-2-(dimethylamino)naphthalene and 6-palmitoyl-2-[[trimethylammoniumethyl]methylamino]naphthalene chloride in vesicles composed of 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine or egg yolk lecithin have been compared using steady-state and time-resolved fluorescence as well as high resolution NMR measurements. The reconstructed time-resolved emission spectra show unambiguously that the observed spectral shifts in vesicle systems have to be assigned to time-dependent solvent relaxation processes rather than to a probe relocation mechanism. All fluorescence as well as the NMR relaxation data suggest a deeper localization of Patman in the membrane, sensing a less polar and/or more restricted probe environment.
Hutterer
R
R
Institute for Physical Chemistry, University of Wuerzburg, Germany.
Schneider
F W
FW
Sprinz
H
H
Hof
M
M
D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D005456:Fluorescent Dyes; D009682:Magnetic Resonance Spectroscopy; D010169:Palmitic Acids / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011522:Protons; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D010169:Palmitic Acids; D010743:Phospholipids; D011522:Protons; C103351:patman; C038517:prodan; D015081:2-Naphthylamine