Effect of serum from partially hepatectomized cirrhotic and noncirrhotic rats on liver regeneration with primary hepatocyte cultures.
The effects of serum from cirrhotic and noncirrhotic rats after 33% and 70% partial hepatectomy on the hepatocyte culture were studied. Liver cirrhosis was produced by weekly intragastric feeding with carbon tetrachloride (CCl4) for 12-16 weeks. The serum was prepared with blood withdrawn from the rats 2 and 48 h after partial hepatectomy or sham operation. The incorporation of [3H]thymidine in the hepatocyte culture after addition of rat serum (1.0 +/- 10(5) cells/ml) was assessed. We found that the mitogenic effect of the serum at 2 and 48 h after partial hepatectomy was not significant. In the 70% hepatectomy group, the serum from cirrhotic rats had a stronger stimulatory effect to induce hepatocytes in primary culture entering DNA synthesis than that from noncirrhotic rats; but the difference was not statistically significant. The mitogenic effect of the serum from noncirrhotic rats after 33% hepatectomy was significantly lower than that from cirrhotic rats after 33% hepatectomy (p < 0.05). In this series, the serum from the cirrhotic rats after 33% hepatectomy had the strongest mitogenic effect; the serum from the partially hepatectomized rats with cirrhotic and non-cirrhotic livers increased [3H]thymidine uptake into hepatocyte DNA significantly more than control serum or plasma.
Chen
M F
MF
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College, Taipei, Taiwan, ROC.
Hwang
T L
TL
Yu
H C
HC
D000818:Animals; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000502:physiology; D008106:Liver Cirrhosis, Experimental / Q000503:physiopathology*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D017228:Hepatocyte Growth Factor; D004247:DNA