A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution.
We have isolated cDNA clones encoding dihydropyrimidinase (DHPase) from human liver and its three homologues from human fetal brain. The deduced amino acid (aa) sequence of human DHPase showed 90% identity with that of rat DHPase, and the three homologues showed 57-59% aa identity with human DHPase, and 74-77% aa identity with each other. We tentatively termed these homologues human DHPase related protein (DRP)-1, DRP-2 and DRP-3. Human DRP-2 showed 98% aa identity with chicken CRMP-62 (collapsin response mediator protein of relative molecular mass of 62 kDa) which is involved in neuronal growth cone collapse. Human DRP-3 showed 94-100% aa identity with two partial peptide sequences of rat TOAD-64 (turned on after division, 64 kDa) which is specifically expressed in postmitotic neurons. Human DHPase and DRPs showed a lower degree of aa sequence identity with Bacillus stearothermophilus hydantoinase (39-42%) and Caenorhabditis elegans unc-33 (32-34%). Thus we describe a novel gene family which displays differential tissue distribution: i.e., human DHPase, in liver and kidney; human DRP-1, in brain; human DRP-2, ubiquitously expressed except for liver; human DRP-3, mainly in heart and skeletal muscle.
Hamajima
N
N
Department of Pediatrics, Nagoya City University Medical School, Japan.
Matsuda
K
K
Sakata
S
S
Tamaki
N
N
Sasaki
M
M
Nonaka
M
M
D000581:Amidohydrolases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002645:Chickens; D002874:Chromosome Mapping; D018076:DNA, Complementary; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009124:Muscle Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000098855:Collapsin Response Mediator Protein 1
D000581:Amidohydrolases; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C102924:CRMP1 protein, human; D000098855:Collapsin Response Mediator Protein 1; C102926:DPYSL3 protein, human; C095114:collapsin response mediator protein-2; C020047:dihydropyrimidinase