A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum.
The flower-specific cDNA Tomf216 representing an mRNA encoding an osmotin-like protein was identified by the differential screening of a cDNA library produced from mRNA extracted from premeiotic tomato flowers (Lycopersicon esculentum). The cDNA is 894 nucleotides in length and encodes a protein of 252 amino acids. The deduced protein has a molecular mass of 27.2 kDa, an N-terminal region predicted to be a signal sequence, and a predicted molecular mass of 24.7 kDa for the mature protein. The protein contains 16 cysteine residues and has 30% to 32% amino acid sequence identity to pathogenesis-related osmotins. The gene is expressed in flowers and fruit with the highest levels of transcript accumulation in pistils from flowers at anthesis. No expression was detected in vegetative organs.
Chen
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Department of Horticultural Science, University of Minnesota, St. Paul 55108, USA.
Wang
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Smith
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D000595:Amino Acid Sequence; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics
D010940:Plant Proteins; C102953:TomF216 protein, Lycopersicon esculentum; C073139:osmotin protein, Nicotiana tabacum