Synthesis of highly pure dolichyl and cholesteryl esters.
Highly pure dolichyl and/or cholesteryl esters can be synthesised at room temperature by reacting a defined pure dolichol and/or pure cholesterol with an acyl chloride in the presence of pyridine. During the reaction some acyl anhydride originates as a secondary product. It can be hydrolysed by methanolic KOH and removed by a rapid percolation of the reaction mixture through a small alumina column. A final purification of the raw product obtained by silicic-acid/celite column chromatography makes it possible to obtain highly pure esters with a satisfactory yield.
Piretti
M V
MV
Pagliuca
G
G
D002788:Cholesterol Esters / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D004286:Dolichols / Q000138:chemical synthesis*; D004952:Esters
D002788:Cholesterol Esters; D004286:Dolichols; D004952:Esters