[Measurement of proliferation of allergen-stimulated peripheral blood lymphocytes by flow cytometry].
The percentage of S-phase cells in peripheral blood mononuclear cells (PBMC) from 14 atopic and 6 non-atopic subjects was measured by flow cytometry after stimulation with mite allergen (Der f) for 6 days and converted to the stimulation index (S.I.F.: stimulation index measured by flow cytometry). S.I. F. showed a close positive correlation with the serum IgE level (r = 0.67, p = 0.001). S.I.F. in the atopic subjects was significantly higher than in the non-atopic subjects (165.6 +/- 49.8 vs 113.2 +/- 12.2, p < 0.05). S.I.F. in subjects with a high (> or = 100 PRU/ml) IgE-RAST level for mite allergen Der p was significantly higher than that in subjects with a negative RAST level (178.4 +/- 53.8 vs 113.2 +/- 12.2, p < 0.02). Flow-cytometric measurement of the proliferation of PBMC in response to allergenic stimulation may be a useful method for studying the immunological abnormality present in atopic subjects.
Kimura
M
M
Division of Allergy, Clinical Immunology and Infectious Diseases, Shizuoka Children's Hospital.
Tsuruta
S
S
Yoshida
T
T
D000293:Adolescent; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008925:Mites / Q000276:immunology*
D000485:Allergens; D007073:Immunoglobulin E