Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms.
Homozygously Deleted in Pancreatic Cancer 4 (DPC4), a recently identified candidate tumor suppressor gene, was previously shown to be altered in human pancreatic cancers. We examined DPC4 mutation in 30 examples of three other types of gastrointestinal malignancy: 10 esophageal cancers, 10 gastric cancers and 10 colorectal cancers occurring in the preneoplastic condition, ulcerative colitis. The entire coding region of DPC4 (including all 11 exons) was analysed by either direct sequencing of PCR product or the in vitro synthesized protein assay. No coding region mutations of DPC4 were found in any of the samples examined. Our results suggest that inactivation of DPC4 may not be important in the majority of these types of gastrointestinal cancer.
Lei
J
J
Department of Medicine, University of Maryland School of Medicine and Veterans Affairs Hospital, Baltimore 21201, USA.
Zou
T T
TT
Shi
Y Q
YQ
Zhou
X
X
Smolinski
K N
KN
Yin
J
J
Souza
R F
RF
Appel
R
R
Wang
S
S
Cymes
K
K
Chan
O
O
Abraham
J M
JM
Harpaz
N
N
Meltzer
S J
SJ
D003093:Colitis, Ulcerative / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins; D004938:Esophageal Neoplasms / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000235:genetics*; D051901:Smad4 Protein; D013274:Stomach Neoplasms / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D004268:DNA-Binding Proteins; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D015534:Trans-Activators