Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21.
Four exons of a human homologue of a yeast cell division cycle gene (MCM6/mis5, which is thought to encode a DNA replication licensing factor) have been identified 3.3 kb upstream from the start of transcription of the intestinal lactase gene on human chromosome 2q21, initially by similarity to a rat 'intestinal crypt-cell replication factor'. RT-PCR analysis shows, that unlike lactase, MCM6 is not restricted in its tissue distribution and does not show person-to-person variation in the level of expression in adult intestine.
Harvey
C B
CB
MRC Human Biochemical Genetics Unit, Wolfson House (UCL), London, UK.
Wang
Y
Y
Darmoul
D
D
Phillips
A
A
Mantei
N
N
Swallow
D M
DM
D000328:Adult; D000595:Amino Acid Sequence; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D043322:Lactase; D064151:Minichromosome Maintenance Complex Component 6; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D001616:beta-Galactosidase / Q000235:genetics*
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029702:Schizosaccharomyces pombe Proteins; D043322:Lactase; D001616:beta-Galactosidase; C103258:MCM6 protein, human; D064151:Minichromosome Maintenance Complex Component 6; C092300:mcm6 protein, S pombe