Migration of cells with immunoglobulin/c-myc recombinations in lymphoid tissues of mice.
Recombinations between c-myc and immunoglobulin (Ig) sequences that typically occur in pristane-induced mouse plasmacytomas were detected in secondary lymphoid tissues from normal mice, chiefly in the gut-associated lymphoid tissue. Based on the analysis of recombination sequences as clonotypic markers, migration of c-myc recombination-positive cells was observed between Peyer's patches and into the intestine. Treatment of plasmacytoma-susceptible BALB/cAn mice with pristane induced proliferation and migration of these cells into mesenteric lymph node, spleen, and oil granuloma within 7 days. Plasmacytoma-resistant strains of mice (DBA/2N, C3H/HeJ, C57BL/6) differed in that (1) they harbored fewer clones (Ig/c-myc recombinations were detected in 33% of resistant mice versus 91% of BALB/cAn mice after pristane treatment); (2) Ig/c-myc-positive cells were rarely detected in the oil granuloma, and (3) c-myc recombined predominantly with the Ig alpha locus in BALB/cAn mice (72%), but with the Ig mu locus in DBA/2N and in C57BL/6 (67%). The results demonstrate that normal mice generate a large number of lymphocytes with aberrant c-myc in intestinal tissues without developing tumors.
Müller
J R
JR
Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Jones
G M
GM
Janz
S
S
Potter
M
M
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002465:Cell Movement; D004273:DNA, Neoplasm / Q000235:genetics; D004198:Disease Susceptibility; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D007113:Immunity, Innate; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008221:Lymphoid Tissue / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D010954:Plasmacytoma / Q000139:chemically induced / Q000473:pathology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013729:Terpenes; D013997:Time Factors
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D013729:Terpenes; C009042:pristane