Presence of Ca(2+)-sensitive and -insensitive SM22 alpha isoproteins in bovine aorta.
Two proteins (25- and 22-kDa) that cross-react with anti-gizzard SM22 alpha antibody were isolated from bovine aorta. The former, identical to a SM22 alpha homolog reported previously (Kobayashi, R., Kubota, T., and Hidaka, H. (1994) Biochem. Biophys. Res. Commun. 198, 1275-1280), associates with the membrane fraction in the presence of Ca2+ and dissociates in the presence of EGTA, while the latter is insensitive to Ca2+. Peptide mapping and partial sequence analysis revealed that the 22-kDa protein is an isoform of the 25-kDa protein, without 22 amino acid residues from the C-terminus. Immunological analysis of the tissue distribution of these proteins showed that the 25-kDa protein exists in lung and spleen besides aorta, while the 22-kDa protein occurs specifically in the aorta and spleen.
Shishibori
T
T
Department of Chemistry, School of Medicine, Kagawa Medical University, Japan.
Yamashita
K
K
Bandoh
J
J
Oyama
Y
Y
Kobayashi
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001011:Aorta / Q000737:chemistry*; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D003429:Cross Reactions; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D010449:Peptide Mapping; D017421:Sequence Analysis; D014018:Tissue Distribution
D008840:Microfilament Proteins; D009124:Muscle Proteins; C058992:Tagln protein, mouse; C051615:transgelin; D002118:Calcium