Erythrocyte membrane Na+,K+ ATP ase activity can be a marker of liver histopathology.
Erythrocyte membrane Na+,K+: Ca2+ ATP ase activities, cholesterol (CH) phospholipid (PL) composition and erythrocyte glutathione (GSH) contents were determined in controls, in patients with chronic active hepatitis and liver cirrhosis. NA+,K+ ATP ase activities were significantly (P < 0.0001) less in patients with chronic active hepatitis and liver cirrhosis (n = 8, 0.102 +/- 0.02 mumol P/mg protein/hour; n = 8, 0.081 +/- 0.02 mumol P/mg protein/hour) than in controls (n = 10, 0.219 +/- 0.05). Histopathological analysis of liver sections obtained from patients with chronic active hepatitis (n = 3) and liver cirrhosis (n = 2) correlated well with erythrocyte biochemical findings. There was a significant negative correlation between Na+,K+ ATP ase activity and portal fibrosis (P < 0.05, r = -8680). However, further experiments performed on larger study populations are needed to better elucidate this correlation. Therefore, NA+K+ ATP ase activity measurement can be reliable assessment of liver fibrosis.
Kuralay
F
F
Ege University School of Medicine, Department of Biochemistry, Izmir, Turkey.
Tanyalçin
T
T
Kutay
F
F
Yuce
G
G
Ersoz
G
G
Batur
Y
Y
D000328:Adult; D015415:Biomarkers; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005260:Female; D005978:Glutathione / Q000097:blood; D006521:Hepatitis, Chronic / Q000473:pathology; D006801:Humans; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D015415:Biomarkers; D010743:Phospholipids; D002784:Cholesterol; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione