The use of rational mutagenesis to modify the chain length specificity of a Rhizopus delemar lipase.
Haas
M J
MJ
Eastern Regional Research Center, United States Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA.
Joerger
R D
RD
King
G
G
Klein
R R
RR
D002384:Catalysis; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012233:Rhizopus / Q000201:enzymology; D013379:Substrate Specificity; D013912:Threonine / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
D018076:DNA, Complementary; D005656:Fungal Proteins; D011498:Protein Precursors; D013912:Threonine; D014364:Tryptophan; D008049:Lipase