Highly sensitive ELISA for soluble Fas in serum: increased soluble Fas in the elderly.
We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 micrograms/L and the detection limit was 0.01 microgram/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sFas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sFas in 155 healthy subjects, ages 20-69. The mean value of sFas in men (2.50 +/- 0.63 micrograms/L, n = 78) was significantly higher than that in women (2.01 +/- 0.53 micrograms/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sFas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sFas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.
Seishima
M
M
Department of Laboratory Medicine, Gifu University School of Medicine, Japan. seishima@.cc.gifu-u.ac.jp
Takemura
M
M
Saito
K
K
Sano
H
H
Minatoguchi
S
S
Fujiwara
H
H
Hachiya
T
T
Noma
A
A
D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D012016:Reference Values; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D012995:Solubility; D019014:fas Receptor / Q000097:blood*
D019014:fas Receptor