Buffer may be the critical factor in measurement of anti-prothrombin antibody on a gamma-ray-irradiated plate by enzyme-linked immunosorbent assay.
We investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a gamma-ray-irradiated plate to measure aPT.
Matsuda
J
J
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Saitoh
N
N
Tsukamoto
M
M
Gotoh
M
M
Gohchi
K
K
Kawasugi
K
K
D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005188:False Negative Reactions; D005720:Gamma Rays; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D010710:Phosphates / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D011516:Prothrombin / Q000276:immunology*; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000494:pharmacology*; D014325:Tromethamine / Q000494:pharmacology*
D001323:Autoantibodies; D002021:Buffers; D010710:Phosphates; D011136:Polysorbates; D014325:Tromethamine; D012965:Sodium Chloride; D011516:Prothrombin