title: Effect of hemocarboperfusion on organ blood flow and survival in porcine endotoxic shock.
abstract: OBJECTIVE
  To evaluate the effects of hemocarboperfusion on hemodynamics, organ blood flow, and survival in endotoxin shock.
  
  
  METHODS
  Prospective, placebo-controlled, animal trial.
  
  
  METHODS
  Research laboratory in a major university teaching hospital.
  
  
  METHODS
  Pentobarbital-anesthetized pigs.
  
  
  METHODS
  Twenty-eight pentobarbital-anesthetized pigs (18.5 to 22.3 kg) received 100 micrograms/kg of Escherichia coli endotoxin (lipopolysaccharide 0127) over 30 mins. Group 1 animals (n = 14) were controls and had blood diverted through an extracorporeal circuit without activated charcoal for 60 mins after lipopolysaccharide infusion. Group 2 animals (n = 14) underwent nonpulsatile hemocarboperfusion (activated charcoal SCN-1K).
  
  
  RESULTS
  Mean arterial pressure, cardiac output, systemic vascular resistance, mean pulmonary arterial pressure, pulmonary vascular resistance, oxygen delivery, and regional blood flow (radiolabeled microsphere technique) were determined at baseline and every 30 mins for 150 mins. Results are presented as mean +/- SD. Parameters in the two groups were compared by two-way analysis of variance. A p < .05 was considered significant. The survival rate was ten (71%) of 14 animals in group 1 compared with 14 (100%) of 14 animals in group 2 (p < .05, Fisher's exact test). The mean cardiac output at the end of hemocarboperfusion was 1.6 +/- 0.6 L/min in group 1 compared with 3.0 +/- 0.9 L/min in group 2, and remained lower in group 1 animals throughout the experiment. Pulmonary arterial pressure and pulmonary vascular resistance were lower in the hemocarboperfusion-treated animals during and after hemocarboperfusion. Systemic vascular resistance increased by 70% after lipopolysaccharide infusion and returned to baseline values in the hemocarboperfusion group but remained increased in controls. Oxygen delivery was lower in group 1 at 90 and 150 mins (287 +/- 34 vs. 478 +/- 48 mL/min and 251 +/- 24 vs. 356 +/- 21 mL/min, respectively). Blood flow rates to the brain (38.5 +/- 7.5 vs. 27.1 +/- 5.4 mL/min/100 g), large intestine (26.6 +/- 1.1 vs. 17.7 +/- 2.5 mL/ min/100 g), and adrenal cortex (200 +/- 45 vs. 139 +/- 41 mL/min/100 g) were higher in the hemocarboperfusion group at the completion of carboperfusion but not at later time points.
  
  
  CONCLUSIONS
  These data suggest that hemocarboperfusion may be of value in the treatment of septic shock.
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, Johns Hopkins Medical Institutions.
authors.forename: V A
authors.initials: VA
authors.lastname: Odnopozov
authors.forename: P F
authors.initials: PF
authors.lastname: Laterre
authors.forename: R J
authors.initials: RJ
authors.lastname: Traystman
authors.forename: E K
authors.initials: EK
authors.lastname: Tsybul'kin
authors.forename: R A
authors.initials: RA
authors.lastname: Winchurch
authors.forename: M J
authors.initials: MJ
authors.lastname: Breslow
mesh_terms: D000818:Animals; D002606:Charcoal / Q000008:administration & dosage; D004927:Escherichia coli Infections / Q000628:therapy*; D005260:Female; D006439:Hemodynamics; D006464:Hemoperfusion; D008297:Male; D012039:Regional Blood Flow; D012772:Shock, Septic / Q000628:therapy*; D013534:Survival; D013552:Swine
chemical_list: D002606:Charcoal