Excimer laser effects on human corneal endothelium. Modulation by serum factor(s).
OBJECTIVE
To determine the possibility of endothelial cell damage after excimer laser ablation.


METHODS
Endothelial cell densities and morphology of human corneas after photoablations or mechanical keratectomy were compared with those of the untreated mates after 1 week of culture with or without serum.


RESULTS
Corneas cultured in serum-free medium after ablation to a depth of 150 microns showed endothelial cell densities reduced to 60% of untreated, mate corneas; ultrastructural analysis showed endothelial cell damage not seen in untreated mates. Corneas ablated to the same depth and cultured in serum-enriched medium showed no endothelial cell density loss, nor did corneas cultured in serum-free medium after an ablation to a depth of 50 microns or mechanical keratectomies averaging 95 microns.


CONCLUSIONS
Endothelial cell loss in deep laser resections may be prevented by factor(s) in fetal bovine serum. The apparent lack of cell loss in clinical studies may be related to the protective action of similar factors in aqueous humor.
Lambert
R W
RW
National Vision Research Institute, San Diego, Calif, USA.
Anderson
J A
JA
Heitzmann
J
J
Sutherland
C J
CJ
Moore
M M
MM
Binder
P S
PS
D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins / Q000494:pharmacology*; D002452:Cell Count; D003315:Cornea / Q000601:surgery* / Q000648:ultrastructure; D003316:Corneal Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D054018:Lasers, Excimer; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D018903:Photorefractive Keratectomy / Q000009:adverse effects*; D014945:Wound Healing
D001798:Blood Proteins; D003470:Culture Media; D016895:Culture Media, Serum-Free