[Lux-regulon from Vibrio fisheri reduces type 1 restriction in Escherichia coli K12 cells].
The EcoK restriction of nonmodified phage lambda.0 controlled by Escherichia coli K12 lon- cells is alleviated 10-20-fold in the presence of a hybrid plasmid containing the entire lux regulon of Vibrio fischeri. The La protease participates in degradation of the regulatory LuxR-LuxI proteins; therefore, transcription of the right lux operon begins significantly earlier than in the lon+ bacteria and is characterized by high content of the N-(3-oxohexanoyl) homoserine lactone autoinducer in medium. The effect of lux-induced alleviation of type I restriction in the lon mutants of E.coli K12 is proposed to depend on the reduction at the intracellular level of S-adenosylmethionine, one of the LuxI synthase substrates responsible for autoinducer synthesis.
Zavil'gel'skiĭ
G B
GB
Manukhov
I V
IV
Rastorguev
S M
SM
D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015253:Deoxyribonucleases, Type I Site-Specific / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D010957:Plasmids; D012097:Repressor Proteins; D015183:Restriction Mapping; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*; D014733:Vibrio / Q000235:genetics*
D001426:Bacterial Proteins; D004279:DNA, Viral; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C064778:LuxR autoinducer binding proteins; D015253:Deoxyribonucleases, Type I Site-Specific
Lux-regulon Vibrio fisheri oslabliaet restriktsiiu 1-go tipa v kletkakh Escherichia coli K12.