title: epsilon-N-acetylation in the production of recombinant human basic fibroblast growth factor mutein.
abstract: During large scale preparation of recombinant human basic fibroblast growth factor (hbFGF) mutein CS23(CS23) produced in Escherichia coli (E. coli), three species, named Mf-1, Mf-2 and Mf-3 in the order of their elution, were isolated from the early fraction of Sulfated Cellulofine chromatography by high performence liquid chromatography (HPLC) on heparin-5pw. Structural analysis through HPLC peptide mapping, mass spectrometry, sequencing and amino acid composition revealed that epsilon-N-acetylation occurred at positions Lys135, Lys26 and Lys119 of CS23. Together with this observation, the results on large scale preparation of CS23 were also presented.
authors.affiliation: Biotechnology Department, Takeda Chemical Industries, Ltd., Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suenaga
authors.forename: H
authors.initials: H
authors.lastname: Ohmae
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Koyama
authors.forename: O
authors.initials: O
authors.lastname: Nishimura
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; C065198:protein CS23; D005346:Fibroblast Growth Factors