title: The bluF gene of Rhodobacter capsulatus is involved in conversion of cobinamide to cobalamin (vitamin B12).
abstract: The bluF gene of Rhodobacter capsulatus is the first gene of the bluFEDCB operon which is involved in late steps of the cobalamin synthesis. To determine the function of the bluF gene product, a bluF::omega-Km mutant strain was constructed and characterized. This vitamin B12 auxotrophic mutant strain shows a 3.5-times higher vitamin B12 requirement under phototrophic growth conditions than under chemotrophic growth conditions. Surprisingly, the bluF promoter activity does not respond to alterations to the oxygen tension or vitamin B12 concentration.
authors.affiliation: Institut für Mikro- und Molekularbiologie, Giessen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Pollich
authors.forename: C
authors.initials: C
authors.lastname: Wersig
authors.forename: G
authors.initials: G
authors.lastname: Klug
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003038:Cobamides / Q000378:metabolism*; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016354:Rhodobacter capsulatus / Q000235:genetics* / Q000378:metabolism; D014805:Vitamin B 12 / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D003038:Cobamides; D004269:DNA, Bacterial; C017142:cobinamide; D014805:Vitamin B 12