Protective effect of adenylate deaminase (from Penicillium lanoso-viride) against acute infections in mice.
We examined the effects of the immunomodulator-adenylate deaminase (E.C. 3.5.4.6) from Penicillium lanoso-viride on experimental mice infections. Prophylactic intraperitoneal administration of adenylate deaminase (ADA) increased survival time and numbers of survivors after infection with Salmonella typhimurium, Pseudomonas aeruginosa and influenza A (H3N2) virus. Protection against influenza virus after intranasal ADA application was also observed. The influence of ADA was time and dose dependent. The most pronounced protection was obtained by administration of 3 U ADA/mice 24 h prior to infection. ADA had no antibiotic effect against these bacterial strains. Protective effects of ADA were studied in immunosuppressed mice under different regimes of treatment including cyclosporin A and trypan blue. The results indicated, that the protective effect of ADA is of a complex nature and probably depends on both T-cell and macrophage components.
Nikolajeva
V
V
Department of Microbiology, Faculty of Biology, University of Latvia, Latvia.
Eze
D
D
Kamradze
A
A
Indulena
M
M
Muiznieks
I
I
D000659:AMP Deaminase / Q000096:biosynthesis* / Q000627:therapeutic use*; D000208:Acute Disease; D000818:Animals; D005260:Female; D009980:Influenza A virus / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000517:prevention & control*; D010407:Penicillium / Q000378:metabolism*; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000188:drug therapy / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000187:drug effects
D000659:AMP Deaminase