Multiplex PCR for diagnosis of AIDS-related central nervous system lymphoma and toxoplasmosis.
A nested multiplex PCR assay was designed for the simultaneous detection of Epstein-Barr virus and Toxoplasma gondii DNA from the cerebrospinal fluid of AIDS patients. T. gondii DNA was detected in 8 of 8 patients with Toxoplasma encephalitis and in 0 of 6 patients without toxoplasmosis, and Epstein-Barr virus DNA was found in 9 of 14 patients with central nervous system lymphoma and in 2 of 38 patients without disease.
Roberts
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Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Storch
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GA
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000469:parasitology*; D000818:Animals; D001932:Brain Neoplasms / Q000150:complications / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000150:complications / Q000469:parasitology*