Functional analysis of a Brassica oleracea SLR1 gene promoter.
The Brassica oleracea S-locus-related gene 1 (SLR1) is expressed in the papillar cells of Brassica stigmas from a few days before anthesis. We have previously shown that a 1500-bp fragment of the SLR1 gene promoter is sufficient to direct high-level, temporally regulated expression of the beta-glucuronidase reporter gene in the pistils of transgenic tobacco. We have carried out a deletion analysis of the SLR1 promoter and found that elements required for pistil expression are located between -258 and -327 bp (relative to the translation start site). Furthermore, specific binding of pistil nuclear factors to sequences within this region was demonstrated by gel retardation analysis. Sequences between -1350 and -1500 were found to be required for high-level expression.
Hackett
R M
RM
School of Biological Sciences, University of Birmingham, Edgbaston, United Kingdom.
Cadwallader
G
G
Franklin
F C
FC
D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D018744:DNA, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic
D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C076705:SLR1 protein, Brassica; D005966:Glucuronidase