Morphometric and metabolic profile of the distal segment of the internal mammary artery: caution on its use for coronary anastomoses.
OBJECTIVE
Elastic arteries were found to be less prone to intimal hyperplasia than muscular arteries. The internal mammary artery (IMA), which is elastic in its proximal segment, presents a gradual decrease of media elastic fibers along its downstream course. Metabolic and morphometric studies of the distal end of the IMA with regard to its local susceptibility to develop intimal changes were undertaken in order to evaluate the reliability of its use as an anastomotic site for bypass grafting.


METHODS
Twenty distal segments of IMA were harvested from patients who had undergone myocardial revascularization. Histologic, enzyme-histochemical and morphometric studies were undertaken on these arterial segments.


RESULTS
Histologic examinations indicated an elastomuscular structure in 13 patients, a muscular structure in 6 and an elastic structure in 1. Of the 20 IMAs, none was found to have intimal thickening of greater than 25% of the diameter of the lumen. The enzyme-histochemical profile of the proliferating cells found in the intimal thickening differed from normal contractile smooth muscle medial cells in the loss of myosin and mitochondrial ATPase, plasma membrane 5' nucleotidase, moderately decreased aerobic dehydrogenase and increased lactate dehydrogenase activity and ribonucleoprotein-linked pyroninophilia. Lysosomal beta-glucuronidase and sulfatase were strongly active. This enzyme behavior is unfavorable to contractile function and favorable to cell proliferation and lipid accumulation, two events strongly involved in the atherogenic process.


CONCLUSIONS
Intimal proliferative changes were observed in the distal segment of the IMA. Although there was no histologic evidence of atherosclerotic plaque, the enzyme-histochemical profile of this intimal thickening was favorable to cell proliferation and lipid accumulation. These findings suggest that it may be beneficial to avoid coronary anastomoses with the distal end of the IMA and to use a more proximal/elastic segment.
Nataf
P
P
Department of Cardiovascular Surgery, La Pitié Hospital, Paris, France.
Hadjiisky
P
P
Bourbon
A
A
Peuchmaurd
M
M
Leprince
P
P
Regan
M
M
Escolano
S
S
Gandjbakhch
I
I
D015720:5'-Nucleotidase / Q000032:analysis; D001192:Arylsulfatases / Q000032:analysis; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis; D000252:Calcium-Transporting ATPases / Q000032:analysis; D003327:Coronary Disease / Q000201:enzymology / Q000473:pathology; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007387:Internal Mammary-Coronary Artery Anastomosis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D008323:Mammary Arteries / Q000201:enzymology* / Q000473:pathology*; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000473:pathology; D009252:NADPH Dehydrogenase / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D009252:NADPH Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D015720:5'-Nucleotidase; D001192:Arylsulfatases; D005966:Glucuronidase; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases