Temperature dependence of NMR relaxation times of nucleoside triphosphates and inorganic phosphate in the isolated perfused rat liver. Effect on Pi compartmentation.
The effect of temperature on 31P NMR spectra from isolated perfused rat livers was studied at 9.4 T. Relaxation times (T1 and T2) of uncleoside triphosphates (NTP) and inorganic phosphate (Pi) were determined at 37, 25, 15, and 4 degrees C. Under hypothermic conditions, an unexpected apparent line sharpening in the Pi spectral region and a clear emergence of an additional Pi resonance were observed. This additional signal was assigned to mitochondrial Pi. T1 values obtained for cytosolic and mitochondrial Pi at 4 degrees C were 1.14 +/- 0.24 s (n = 5) and 0.71 +/- 0.18.s (n = 5), respectively. No significant mitochondrial contribution to the Pi resonance was observed at 37 degrees C. Quantification of Pi and NTP liver contents at 37 and 4 degrees C was performed by comparing the perfused liver spectrum and the corresponding perchloric acid extract spectrum. Under experimental conditions of low external Pi (0.12 mM), it was concluded that intracellular Pi was completely NMR-visible at 4 and 37 degrees C. The observation of the mitochondrial Pi signal at 4 degrees C was well explained by an increase in the Pi level within the matrix, in response to the mitochondrial swelling induced by hypothermia, as observed by electron microscopy. T2 values for the cytosolic Pi at 37 and 4 degrees C were 17 +/- 4 ms (n = 8) and 22 +/- 4 ms (n = 10), respectively. Comparison with measured linewidths indicated that line broadening for the main phosphorylated metabolites--including matrix Pi--was the result of B0 field inhomogeneity. The additional broadening of the cytosolic Pi resonance at 4 and 37 degrees C was attributed to pH heterogeneity within the liver.
Dufour
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S
Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS, Université de Bordeaux, France.
Thiaudière
E
E
Vidal
G
G
Gallis
J L
JL
Rousse
N
N
Canioni
P
P
D000818:Animals; D003600:Cytosol / Q000737:chemistry; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry* / Q000302:isolation & purification; D010472:Perchlorates; D010477:Perfusion; D010710:Phosphates / Q000737:chemistry* / Q000302:isolation & purification; D010759:Phosphorus Isotopes; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
D009705:Nucleosides; D010472:Perchlorates; D010710:Phosphates; D010759:Phosphorus Isotopes