Mast cell activation accelerates the normal rate of angiogenesis in the chick chorioallantoic membrane.
Using shell-less cultures of chick embryos, the effect of mast cell degranulation on normal rates of angiogenesis in the chorioallantoic membrane (CAM) was recorded from Day 6 to Day 9. Based on the centripetal ordering method with capillaries as the initial point of reference, the first two orders of pre- and postcapillary microvessels were evaluated. After infusion of compound 48/80 into the allantoic cavity, the normal proliferation rate of order-1 pre- and postcapillaries was markedly increased. Increases of second-order vessel numerical densities (number/cm2) were less than those of the order-1 vessels. Thus, length densities (mean length x numerical density) were increased for the order-1 vessels only. During the accelerated rate of CAM angiogenesis, endothelial restriction of FITC-dextran 150 remained uniformly high. Hence, secreted mast cell products likely worked synergistically with endogenous CAM angiogenic factors between Days 6 and 9, without increasing rates of macromolecular extravasation. However, histamine injection into the allantoic cavity at Day 10, in conjunction with calcium supplementation of the shell-less cultures at Day 9, elevated the rate of FITC-dextran 150 extravasation. Whether differentiation of this transendothelial pathway is required for enhancement of CAM angiogenesis after Day 10 remains uncertain.
Rizzo
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Department of Anatomy, Cell Biology, and Injury Sciences, UMDNJ-New Jersey Medical School, Newark 07103-2714, USA.
DeFouw
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D000818:Animals; D002642:Chick Embryo; D004625:Embryo, Nonmammalian / Q000098:blood supply*; D006632:Histamine / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000502:physiology*
D006632:Histamine