Identification and molecular cloning of a 67-kilodalton protein in Schistosoma japonicum homologous to a family of actin-binding proteins.
A monoclonal antibody to Schistosoma japonicum which conferred significant protection against cercarial challenge in mice was produced. The predicted translation product of the cDNA corresponding to the antigen recognized by this antibody was homologous to a newly identified family of actin-binding proteins. The expressed protein bound polymerized actin and was recognized by serum from patients infected with S. japonicum.
Kurtis
J D
JD
International Health Institute, Brown University, Providence, Rhode Island, USA.
Ramirez
B L
BL
Wiest
P M
PM
Dong
K L
KL
El-Meanawy
A
A
Petzke
M M
MM
Johnson
J H
JH
Edmison
J
J
Maier
R A
RA
Olds
G R
GR
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D015801:Helminth Proteins / Q000235:genetics*; D007116:Immunization, Passive; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D012549:Schistosoma japonicum / Q000235:genetics*; D012554:Schistosomiasis japonica / Q000517:prevention & control; D017386:Sequence Homology, Amino Acid; D000099149:Ezrin
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D000099149:Ezrin; C103355:JF antigen, Schistosoma japonicum; C071776:radixin