title: Peroxidases, lactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis.
abstract: OBJECTIVE
  The aim of this study was to examine the longitudinal association of selected non-immune anti-microbial host factors (peroxidases, lysozyme and lactoferrin) to the localized juvenile periodontitis (LJP) disease status.
  
  
  METHODS
  Peroxidases, lysozyme and lactoferrin were quantitated from seven patients with LJP before and after periodontal therapy. Analyses were performed from simultaneously collected samples of peripheral blood polymorphonuclear leukocytes (PMNs), gingival crevicular fluid (GCF from diseased sites) and paraffin-stimulated whole saliva. Similar assays were done also from seven periodontally healthy controls.
  
  
  RESULTS
  During untreated phase of LJP myeloperoxidase, lysozyme and lactoferrin concentrations were remarkably elevated in peripheral blood PMNs, also reflected in their high concentrations in GCF. All these values normalised with respect to healthy controls during the periodontal therapy. No similar longitudinal changes were seen in whole saliva but during therapy salivary peroxidase concentrations declined below the control values, in accordance with our previous observations in parotid saliva samples of LJP patients.
  
  
  CONCLUSIONS
  In LJP the concentrations of lysozyme, lactoferrin and myeloperoxidase are significantly elevated in peripheral blood PMNs, also reflected in GCF. During periodontal therapy these values decline and approach those observed in healthy controls. No similar changes are seen in stimulated whole saliva.
authors.affiliation: Department of Periodontology, University of Helsinki, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Suomalainen
authors.forename: L
authors.initials: L
authors.lastname: Saxén
authors.forename: P
authors.initials: P
authors.lastname: Vilja
authors.forename: J
authors.initials: J
authors.lastname: Tenovuo
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000097:blood / Q000201:enzymology / Q000378:metabolism* / Q000628:therapy; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000201:enzymology; D006801:Humans; D007781:Lactoferrin / Q000032:analysis / Q000097:blood; D008297:Male; D009113:Muramidase / Q000032:analysis / Q000097:blood; D009504:Neutrophils / Q000737:chemistry / Q000201:enzymology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000473:pathology; D010544:Peroxidases / Q000032:analysis / Q000097:blood; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology; D013997:Time Factors
chemical_list: D010544:Peroxidases; D009113:Muramidase; D007781:Lactoferrin