title: Flow cytometry evaluation of the in vitro influence of four i.v. anaesthetics on respiratory burst of neutrophils.
abstract: Exposure of neutrophils to anaesthetic agents may alter their functional characteristics and in patients undergoing long-term sedation this may be clinically relevant. We have investigated the in vitro influence of propofol, thiopentone, methohexitone and midazolam on phorbol 12-myristate 13-acetate (PMA)-induced respiratory burst of neutrophils by the intracellular oxidative transformation of dihydrorhodamine-123 to the fluorescent dye rhodamine-123 via flow cytometry. We tested in vitro concentrations similar to sedating, anaesthetic, 10-fold sedating and 10-fold anaesthetic plasma concentrations. All drugs showed similar inhibition of respiratory burst at sedating concentrations (1-6%). At anaesthetic concentrations, propofol produced significantly higher mean inhibition (7.3%) compared with thiopentone (4.5%) and methohexitone (0.9%). At 10-fold anaesthetic concentrations inhibition of respiratory burst by propofol was almost complete (90.8%) and significantly higher than that by thiopentone (29.2%) and methohexitone (1.8%). Methohexitone and midazolam had only minimal effects at all concentrations. The effect of the solvent of propofol (10% Intralipid) was similar to that of propofol. Thus suppression of respiratory burst of neutrophils by propofol may be caused by this lipid carrier.
authors.affiliation: Department of Anaesthesiology, Hannover Medical School.
authors.forename: J
authors.initials: J
authors.lastname: Heine
authors.forename: M
authors.initials: M
authors.lastname: Leuwer
authors.forename: D
authors.initials: D
authors.lastname: Scheinichen
authors.forename: L
authors.initials: L
authors.lastname: Arseniev
authors.forename: K
authors.initials: K
authors.lastname: Jaeger
authors.forename: S
authors.initials: S
authors.lastname: Piepenbrock
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008723:Methohexital / Q000494:pharmacology; D008874:Midazolam / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects*; D015742:Propofol / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D013874:Thiopental / Q000494:pharmacology
chemical_list: D018686:Anesthetics, Intravenous; D008723:Methohexital; D013874:Thiopental; D008874:Midazolam; D015742:Propofol