Increased interferon-gamma mRNA expression following alloincompatible myoblast transplantation is inhibited by FK506.
Myoblasts from C57BL/10SnJ+/+ were transplanted in major histocompatibility complex (MHC)-compatible mice (i.e., C57BL10SnJ+/+ and C57BL/10SnSc mdx/mdx) and in MHC-noncompatible (BALB/c+/+) mice. The recipients were killed 1-21 days after transplantation. C57BL10SnJ+/+ myoblasts were also transplanted in a few BALB/c+/+ mice treated with FK506 and killed 7 days thereafter. Our results showed that after MHC-noncompatible transplantation, interferon-gamma (IFN-gamma) mRNA expression is increased from day 5 to day 21, indicating the presence of a cellular immune reaction. Short-term immunosuppressive treatment with FK506 inhibited the transcription of IFN-gamma mRNA compared with that in untreated mice. Myoblast-specific antibodies were also detected 2 and 3 weeks after MHC-incompatible transplantation, indicating that the cellular immune reaction, revealed by the increase in IFN-gamma, was followed by a humoral reaction.
Guérette
B
B
Centre de Recherche en Neurobiologie, Hôpital de l'Enfant Jésus, Université Laval, Québec, Canada.
Tremblay
G
G
Vilquin
J T
JT
Asselin
I
I
Gingras
M
M
Roy
R
R
Tremblay
J P
JP
D000818:Animals; D017690:Cell Transplantation; D006648:Histocompatibility; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000166:cytology*; D016133:Polymerase Chain Reaction; D011184:Postoperative Period; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016559:Tacrolimus / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D007371:Interferon-gamma; D016559:Tacrolimus