Coexpression of hepatocyte growth factor and its receptor (c-met oncogene) in HGL4 glioblastoma cells.
Many recent studies have shown that hepatocyte growth factor (HGF) is pleiotropic. Indeed, HGF has been reported to act as a mitogen and a morphogen as well as a mitogen. To evaluate the biological significance of HGF in many cell types of different origin, we tested 32 cell lines for expression of HGF mRNA. Of 32 cell lines, 6 lines expressed HGF mRNA. Two glioblastoma cell lines, HGL4 and U138, also expressed its specific receptor (c-met oncogene) mRNA. The conditioned media of HGL4 contained 3.7-fold higher HGF activity compared to FaONeO cells as a negative control. Recombinant HGF stimulated DNA synthesis of HGL4 cells in a dose-dependent manner. In addition, anti-HGF antibody inhibited DNA synthesis of these cells. These findings suggest that coexpression of HGF and c-met oncogene stimulates growth of HGL4 glioblastoma cells.
Shiota
G
G
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Kawasaki
H
H
Nakamura
T
T
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005909:Glioblastoma / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D019859:Proto-Oncogene Proteins c-met; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology
D012333:RNA, Messenger; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases