title: Volume regulation in rat brain glial cells: lack of a substantial contribution of free amino acids.
abstract: Volume regulation of C6 glioma cells was studied while the bath osmolality was reduced from 300 to 150 mosmol/kg. Exposure to a hyposmotic challenge elicited a typical regulatory volume decrease (RVD). No regulatory volume increase was observed upon restoration of isosmotic conditions. During a second subsequent hyposmotic challenge, the cells did not respond with RVD. High extracellular K+ concentration and the K+ channel blockers Ba2+ and quinine inhibited the RVD. RVD was abolished after Cl- was replaced by gluconate and by the Cl- channel blocker 5-nitro-2(3-phenylpropylamino)benzoic acid. Amino acid (AA) concentration in cell and perfusate was determined. In control, cell content was only 26 mmol/l. Hypotonicity increased the efflux of AA from 0.14 to 0.60 mmol/min. During the second hyposmotic challenge, the release was 0.32 mmol/min. The data show that C6 cells adjust their volume under hyposmotic conditions but lose the ability to restore their volume during a subsequent hyposmotic treatment. K+ and Cl- are the main osmolytes involved in volume adjustment through conductive pathways. AA do not contribute substantially to cell volume regulation.
authors.affiliation: Laboratory of Physiology, K.U. Leuven, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Mountian
authors.forename: P E
authors.initials: PE
authors.lastname: Declercq
authors.forename: W
authors.initials: W
authors.lastname: Van Driessche
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000166:cytology*; D002712:Chlorides / Q000502:physiology; D007038:Hypotonic Solutions / Q000494:pharmacology; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D011188:Potassium / Q000502:physiology; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D000596:Amino Acids; D002712:Chlorides; D007038:Hypotonic Solutions; D011188:Potassium