title: An acute intake of phosphate increases parathyroid hormone secretion and inhibits bone formation in young women.
abstract: We studied the effects of a single oral phosphate (Pi) dose as well as those of three consecutive oral phosphate doses on calcium and bone metabolism. In the first part of the study (P1 study) 10 female volunteers were given orally 1500 mg of Pi in water, as a single dose, or plain water in randomized order at two different sessions. In the second part of the study (P3 study), 10 female volunteers were given orally 1500 mg of Pi, as three separate 500 mg doses in water, or plain water in randomized order. Calcium and bone metabolism was monitored for 24 h by measuring the concentrations of serum ionized calcium (S-iCa), urinary calcium, serum phosphate (S-P), urinary P, serum intact parathyroid hormone (PTH), serum carboxy-terminal propeptide of type I collagen (PICP), serum osteocalcin (BGP), serum carboxy-terminal telopeptide of type I collagen (ICTP), urine deoxypyridinoline (DPD) and bone-specific alkaline phosphatase activity (B-ALP). The S-P increased (p = 0.00005 and p = 0.0005, in the P1 and P3 studies, respectively), the S-iCa concentration declined significantly only in the P1 study (p = 0.0014), the urinary calcium excretion decreased (p = 0.02 and 0.013, in the P1 and P3 studies, respectively), and the PTH concentration rose (p = 0.0083 and p = 0.014, in the P1 and P3 studies, respectively) during the phosphate experiment as compared with the control session. Of the three markers of bone formation studied, PICP declined in the P1 study (p = 0.04), and B-ALP declined in both parts of the study (p = 0.027, p = 0.026, in the P1 and P3 studies, respectively) after phosphate administration, whereas there was no significant change in BGP in either of the studies. The markers of bone resorption, ICTP and DPD, were unaffected by the phosphate load in both studies. In conclusion, acute ingestion of phosphate leads to an increase in S-P, a decrease in S-iCa, and an increase in intact PTH secretion. Our results indicate that these events may lead to an acute inactivation of the early phases of bone formation. In this setting, there was no indication of enhanced bone resorption despite the increase in PTH secretion, which could be due to the combined effect of phosphate and PTH on bone resorption.
authors.affiliation: Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kärkkäinen
authors.forename: C
authors.initials: C
authors.lastname: Lamberg-Allardt
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001846:Bone Development / Q000187:drug effects*; D001862:Bone Resorption / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006706:Homeostasis; D006801:Humans; D008903:Minerals / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism*; D010710:Phosphates / Q000008:administration & dosage*; D012016:Reference Values
chemical_list: D015415:Biomarkers; D008903:Minerals; D010281:Parathyroid Hormone; D010710:Phosphates; D002118:Calcium