Use of random amplified polymorphic DNA (RAPD) technique in inheritance studies of Plasmodium falciparum.
Effectiveness of random amplified polymorphic DNA (RAPD), a technique using 1 10-base primer to amplify random segments of genomic DNA, and some of its possible uses were tested in the A + T-rich genome of Plasmodium falciparum. The best concentrations of MgCl2, 60% G + C primer, and DNA were determined to be 4.0 mM, 0.4 microM, and 90-180 ng/15 microliters reaction, respectively. Use of 30% G + C primers did not allow amplification to occur. Application of RAPD to DNA of parent and progeny clones from a P. falciparum cross showed that polymorphisms identified in the parentals and tracked in the progeny were inherited in a Mendelian fashion and that RAPD-identified polymorphisms could be used as genetic markers. Some of these polymorphic markers were located on more than 1 chromosome, whereas others were specific for a single chromosome. Two of these markers, each located on chromosome 3 of 1 of the parental parasites, were missing from 2 of the 18 progeny, suggesting that deletions, or crossover events had occurred. RAPD markers also identified a higher number of nonparental-type progeny than expected, thus confirming previous observations for high genetic variability in malaria parasites.
Howard
J
J
Brigham Young University, Microbiology Department, Provo, Utah 84602, USA.
Carlton
J M
JM
Walliker
D
D
Jensen
J B
JB
D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis*; D005819:Genetic Markers; D018503:Genome, Protozoan; D015636:Magnesium Chloride; D010963:Plasmodium falciparum / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D016054:DNA, Protozoan; D005819:Genetic Markers; D015636:Magnesium Chloride