[Mutator genes of the yeast Saccharomyces cerevisiae. Interaction of mutations him and his with mutations blocking three principal pathways of repair of induced DNA damage].
During recent years, genes controlling mutation in higher eukaryotes have been found to be involved actively in carcinoma regeneration in cells. In this respect, studying the genetic control of mutagenesis becomes a key direction of research into mechanisms responsible for cancer generation. The results of studying interaction of mutations in the HIM and HSM genes, controlling spontaneous and induced mutagenesis in yeasts, and mutations impairing three known pathways of DNA damage repair in this microorganism, are described in this work. It was shown that mutation rev3 completely blocks UV-induced mutagenesis in all mutants studied. On the other hand, mutation rad2 synergistically interacts with mutations him1, hsm1, hsm3, hsm6, and hsm2, thus enhancing the frequency of UV-induced mutagenesis in double mutants multiple times. Mutations him2 and him3 manifested epistatic interaction with mutation rad2. With mutation rad54, the interaction was epistatic for mutations him1 and hsm2 and was additive for mutations hsm1, him2, and him3. On the basis of the data obtained, we developed a scheme for the appearance of mismatch bases in the process of repair of UV-induced DNA damage.
Koval'tsova
S V
SV
Gracheva
L M
LM
Evstiukhina
T A
TA
Fedorova
I V
IV
Alekseev
S Iu
SIu
Korolev
V G
VG
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D009154:Mutation; D011836:Radiation Tolerance; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects
D004271:DNA, Fungal
Geny-mutatory drozhzheĭ Saccharomyces cerevisiae. Vzaimodeĭstvie mutatsiĭ him i hsm s mutatsiiami, blokiruiushchimi tri osnovnykh puti reparatsii indutsirovannykh povrezhdeniĭ DNK.