Identification of metallothionein isoforms with capillary zone electrophoresis using a polyacrylamide-coated tube.
Metallothionein (MT) isoforms from various liver tissues were separated with capillary zone electrophoresis (CZE) using a polyacrylamide-coated tube at neutral pH. The electrophoresis was performed on MT-1 and MT-2 purified from mouse, rat, rabbit and human livers. The retention times of mouse and rat MT-1 coincided, while the retention times of rabbit and human MT-1 were longer. The retention times of MT-2 purified from the four sources were the same. MT-1 and MT-2 separated more definitely with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-Tris buffer (25 mM, pH 7.4) than with N-tris(hydroxymethyl)methyl-3-aminopropane sulfonic acid (TAPS)-Tris buffer (25 mM, pH 7.7) or with N-(2-acetamido)iminodiacetic acid (ADA)-Tris buffer (25 mM, pH 7.4). In addition, liver MT isoforms prepared from Zn- or Cd-administered mice could be separated.
Minami
T
T
Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Matsubara
H
H
O-higashi
M
M
Otaki
N
N
Kimura
M
M
Kubo
K
K
Okabe
N
N
Okazaki
Y
Y
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002021:Buffers; D002104:Cadmium / Q000008:administration & dosage / Q000494:pharmacology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007279:Injections, Subcutaneous; D008099:Liver / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D011817:Rabbits; D051381:Rats; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002021:Buffers; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc; D000099166:Polyacrylamides