Transcriptional downregulation of a mouse Golgi mannosidase gene following Salmonella typhimurium uptake into murine macrophages.
Uptake of Salmonella typhimurium into murine macrophage cells caused transcriptional downregulation of a mRNA population in eukaryotic cells after 2 h of infection. The cloned cDNA that was identified showed extensive homology with a murine alpha 1,2-mannosidase gene involved in N-oligosaccharide processing. These findings suggest a disruption of eukaryotic protein processing after phagocytosis of S. typhimurium by the murine macrophage cells.
Schwan
W R
WR
Laboratory of Enteric and Sexually Transmitted Diseases, Food and Drug Administration-Center for Biologics Evaluation and Research, Bethesda, MD 20892, USA. bschwan@path.path.com
Kopecko
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line / Q000201:enzymology; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000201:enzymology* / Q000382:microbiology*; D008361:Mannosidases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D043323:alpha-Mannosidase
D008361:Mannosidases; D043323:alpha-Mannosidase