Difference in signal transduction for IL-10 and IFN-gamma production in a CD8+ T cell clone.
CD8+ T cell clones produced both interleukin 10 (IL-10) and interferon-gamma (IFN-gamma) when these cells were stimulated with a T-cell-specific mitogen, concanavalin A (Con A). One of these CD8+ T cell clones, 13G2, secreted IFN-gamma at similar levels with calcium ionophore, A23187, as well as by Con A, but IL-10 production by A23187 was less than by Con A. On the other hand, N6,O2-dibutyryl cAMP enhanced the production of IL-10 but not IFN-gamma when the low doses of Con A or A23187 coexisted. In a T cell clone, the production of these two cytokines required different signal transductions. These results indicate that a T cell clone can produce diverse cytokines depending on the surrounding condition.
Minai
Y
Y
Department of Applied Biological Chemistry, University of Tokyo, Japan.
Goto
M
M
Kohyama
M
M
Hisatsune
T
T
Nishijima
K I
KI
Kaminogawa
S
S
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002999:Clone Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000187:drug effects / Q000502:physiology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D011493:Protein Kinase C / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D016753:Interleukin-10; D007371:Interferon-gamma; D000242:Cyclic AMP; D011493:Protein Kinase C; D002118:Calcium