NG2 proteoglycan and the actin-binding protein fascin define separate populations of actin-containing filopodia and lamellipodia during cell spreading and migration.
The transmembrane proteoglycan NG2 is able to interact both with components of the extracellular matrix and with the actin cytoskeleton. An examination of the distribution of NG2 during cell spreading suggests that NG2 can associate with two distinct types of actin-containing cytoskeletal structures, depending on the nature of the stimulus derived from the substratum. On fibronectin-coated dishes, cell surface NG2 associates exclusively with stress fibers developing within the cell. On poly-L-lysine-coated dishes, cell surface NG2 is associated with radial processes extending from the cell periphery. Spreading on fibronectin/poly-L-lysine mixtures, as well as on matrix components such as laminin, tenascin, and type VI collagen, produces cells with mosaic characteristics, i.e., NG2 is associated with both types of structures. NG2-positive radial processes are distinct from a second population of radial structures that contain fascin. NG2-positive extensions appear to be individual self-contained units (filopodia), whereas fascin is associated with actin ribs within sheets of membrane (lamellipodia). NG2- and fascin-positive structures are often localized to opposite poles of spreading cells, suggesting a possible role for the two classes of cellular extensions in the establishment of cell polarity during morphogenesis or migration. Time lapse imaging confirms the presence of lamellipodia on the leading edges of migrating cells, while numerous filopodia are present on trailing edges.
Lin
X H
XH
La Jolla Cancer Research Center, Burnham Institute, California 92037, USA.
Grako
K A
KA
Burg
M A
MA
Stallcup
W B
WB
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002451:Cell Compartmentation; D002462:Cell Membrane; D002465:Cell Movement; D003599:Cytoskeleton; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism*; D018715:Microscopy, Video / Q000379:methods; D011107:Polylysine / Q000494:pharmacology; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured; D000099216:Chondroitin Sulfate Proteoglycan 4
D000199:Actins; D000941:Antigens; D002352:Carrier Proteins; D005353:Fibronectins; D008840:Microfilament Proteins; D011107:Polylysine; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C020324:fascin