title: nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli.
abstract: In Escherichia coli ribonucleotide reduction is catalyzed by two separate enzymes during aerobic and anaerobic growth. The aerobic enzyme is coded by the nrdAB genes, the anaerobic enzyme by nrdDG. We now show that knock-out mutants of either nrdD or nrdG cannot grow during strict anaerobiosis, achieved by inclusion of sodium sulfide in the medium. Interestingly, these mutants grow well under microaerophilic conditions by overproducing the aerobic enzyme. Under such conditions wild-type bacteria turn off nrdAB and switch on nrdDG.
authors.affiliation: Department of Genetics and Microbiology, University of Barcelona, Bellaterra, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Garriga
authors.forename: R
authors.initials: R
authors.lastname: Eliasson
authors.forename: E
authors.initials: E
authors.lastname: Torrents
authors.forename: A
authors.initials: A
authors.lastname: Jordan
authors.forename: J
authors.initials: J
authors.lastname: Barbé
authors.forename: I
authors.initials: I
authors.lastname: Gibert
authors.forename: P
authors.initials: P
authors.lastname: Reichard
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006918:Hydroxyurea / Q000494:pharmacology; D009154:Mutation; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012264:Ribonucleotide Reductases / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D014764:Viral Proteins; C490362:NrdG protein, E coli; D012264:Ribonucleotide Reductases; C101141:anaerobic ribonucleotide reductase; D006918:Hydroxyurea