title: Agonistic effects of anti-peptide antibodies and autoantibodies directed against adrenergic and cholinergic receptors: absence of desensitization.
abstract: Affinity-purified autoantibodies and anti-peptide antibodies directed against the second extracellular loop of the beta 1-adrenoceptor increase the beating rate of cultured cardiomyocytes just like the beta-adrenergic agonist isoprenaline. Their positive chronotropic action is blocked by beta-adrenergic antagonists. Affinity-purified autoantibodies and anti-peptide antibodies directed against the muscarinic cholinergic M2 receptor exert in these myocytes, like the muscarinic cholinergic agent carbachol, a negative chronotropic effect that is antagonized by atropine. In contrast to the agonism of isoprenaline and carbachol, the described agonistic effects of the antibodies are not subject to desensitization.
authors.affiliation: Max-Delbrück-Centre for Molecular Medicine, Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Wallukat
authors.forename: M L
authors.initials: ML
authors.lastname: Fu
authors.forename: Y
authors.initials: Y
authors.lastname: Magnusson
authors.forename: A
authors.initials: A
authors.lastname: Hjalmarson
authors.forename: J
authors.initials: J
authors.lastname: Hoebeke
authors.forename: A
authors.initials: A
authors.lastname: Wollenberger
mesh_terms: D000318:Adrenergic beta-Agonists / Q000276:immunology* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D001323:Autoantibodies / Q000276:immunology / Q000494:pharmacology*; D002478:Cells, Cultured; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D043585:Receptor, Muscarinic M2; D011943:Receptors, Adrenergic, beta / Q000276:immunology*; D011976:Receptors, Muscarinic / Q000276:immunology*
chemical_list: D000318:Adrenergic beta-Agonists; D000906:Antibodies; D001323:Autoantibodies; D010455:Peptides; D043585:Receptor, Muscarinic M2; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic