Isolation of viable cells in canine transmissible sarcoma (CTS) using density gradient centrifugation.
Density gradient centrifugation using Ficoll-Conray solution was attempted to isolate viable canine transmissible sarcoma (CTS) cells. The viability of isolated tumor cells increased from about 50% to > 90%, and the yield of CTS cells was > 50% with over 99% purity, in when an isolation solution density of 1.05 was used.
Okamoto
Y
Y
Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan.
Minami
S
S
Matsuhashi
A
A
Fujinaga
T
T
D000818:Animals; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D002470:Cell Survival / Q000502:physiology; D002499:Centrifugation, Density Gradient / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D012509:Sarcoma / Q000473:pathology*; D013046:Specific Gravity; D014685:Venereal Tumors, Veterinary / Q000473:pathology*