title: Flow cytometric crossmatching in renal transplantation--outcome after five years.
abstract: The association of a positive flow cytometric crossmatch between recipient IgG directed against donor T lymphocytes and poor outcome is well described in renal transplantation. Until now, no long-term follow-up on such patients has been available. A total of 117 renal transplant patients were followed up for a period of 5 years. Of these, 21 were known to have donor T cell-directed IgG and 5 had B lymphocyte-directed IgG. Both groups of patients with these antibodies had a significantly poorer outcome at 5 years than did the group of patients without IgG (P < 0.0001 Handel Maenzel test). Patients with antibody detected preoperatively were tested again, either at the time of graft failure or at 5 years posttransplantation. The sera were tested against stored donor cells and the intensity of surface IgG compared with the preoperative levels. In those recipients who lost their grafts, the levels increased in 60% of cases but those that retained their grafts also had an increase in levels of donor-directed antibody in 50% of cases. The changing levels of antibody therefore appeared to have little relevance to outcome. However, when IgG isotypes were considered, for those who experienced graft failure and also had a gamma 3 isotype, a rise in IgG was demonstrated in all cases. Conversely, successful grafts with gamma 3 had a decline in levels between preoperative and 5-year samples in three of the four cases (p not significant).
authors.affiliation: Renal Transplant Unit, Royal Victoria Infirmary, Newcastle-upon-Tyne, UK.
authors.forename: D
authors.initials: D
authors.lastname: Talbot
authors.forename: M
authors.initials: M
authors.lastname: White
authors.forename: B K
authors.initials: BK
authors.lastname: Shenton
authors.forename: A
authors.initials: A
authors.lastname: Bell
authors.forename: D
authors.initials: D
authors.lastname: Manas
authors.forename: G
authors.initials: G
authors.lastname: Proud
authors.forename: R M
authors.initials: RM
authors.lastname: Taylor
mesh_terms: D005434:Flow Cytometry; D006085:Graft Survival; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D012189:Retrospective Studies
chemical_list: D007074:Immunoglobulin G