Precipitation flow injection immunoassay for human immunoglobulin G.
The development of a precipitation flow injection immunoassay is described. This approach is based on the immunoprecipitin reaction, whereby the precipitate formed by binding of the sample antigen (HIgG) to fluorescein-labelled antibodies is retained on a filter built in on-line in a flow-injection system. After dissolving the precipitate with sodium hydroxide solution the liberation of fluorescein-labelled antibodies results in a fluorescence signal that is directly proportional to the concentration of HIgG. The instrumental set-up is very simple and fully automated. One assay cycle takes about 10 min. The RSD ranged between 1 and 3%, depending on concentration. The detection limit was about 140 fmol. The recovery from spiked serum samples was between 96 and 102%.
Hacker
A
A
Institute of Pharmaceutical Chemistry, Karl-Franzens-University of Graz, Austria.
Gübitz
G
G
Fernandez-Romero
J M
JM
Luque de Castro
M D
MD
Valcarcel
M
M
D001331:Automation; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D011233:Precipitin Tests / Q000379:methods*
D005452:Fluoresceins; D007074:Immunoglobulin G; D019793:Fluorescein