A highly precise reporter gene bioassay for type I interferon.
We describe the setting up and validation of a reporter gene assay for type I IFN based on monkey Vero cells transfected with pMx-Luc, a plasmid carrying the luciferase gene under the control of the type I IFN inducible mouse Mx1 promoter. Vero cells were stably transfected with pMx-Luc and clone 3-143/5 was selected on the basis of luciferase inducibility by IFN-beta. A linear dose-response relationship was found between 1 and 16 IU/ml IFN-beta. The assay was shown to be specific for IFN-alpha and -beta as no effect by a number of other cytokines including IFN-gamma could be detected. In order to render the IFN-beta reporter gene assay protocol more suitable for routine assays, a 3 x 3 balanced parallel line assay design was applied using a 96-well luminometer for luminescence measurement. The assay was shown to be precise with a coefficient of variation of less than 9%. This assay is characterized by high precision coupled to high efficiency, as reflected by a very short assay duration (1 day), when compared to the classical cytopathic effect assays for IFNs and the previously published IFN reporter gene assay based on growth hormone measurement (Lleonart, R., Näf, D., Browning, H. and Weissmann, C. (1990) A novel, quantitative bioassay for type 1 interferon using a recombinant indicator cell line. Biotechnology 8, 1263-1267).
Canosi
U
U
Instituto di Ricerca Cesare Serono SpA, Rome, Italy.
Mascia
M
M
Gazza
L
L
Serlupi-Crescenzi
O
O
Donini
S
S
Antonetti
F
F
Galli
G
G
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002522:Chlorocebus aethiops; D002999:Clone Cells; D017930:Genes, Reporter / Q000276:immunology*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D008156:Luciferases / Q000032:analysis; D014709:Vero Cells
D016899:Interferon-beta; D008156:Luciferases