title: Role of T cell subpopulations in mice infected with Angiostrongylus cantonensis.
abstract: When C57BL/6 mice were infected with Angiostrongylus cantonensis, the percentage of T helper (CD4+) cells and T supressor (CD8+) cells in peripheral blood increased weekly until the third and seventh week respectively, and then gradually decreased. C57BL/6 mice were depleted of CD4+ and CD8+ T cells by in vivo injection of anti-CD4 and anti-CD8 monoclonal antibodies, respectively, and then infected with A. cantonensis. There were significantly more and less worms recovered in the mice depleted of CD4+ and CD8+ T cells respectively than in undepleted mice. Discrete subpopulations of T cells from mice exposed to A. cantonensis for 3 weeks or 7 weeks were adoptively transferred to syngeneic recipients which were then given a challenge infection. Protection was mediated by a CD4+ T cell population present in mice after 3 weeks of infection but was not demonstrable with cells taken 7 weeks after infection. When CD4+ T cells obtained from 3-week infected mice were mixed with 5% CD8+ T cells obtained from mice infected for 7 weeks, no significant transfer of resistance was observed. Thus, immune responses to A. cantonensis in mice were regulated by discrete subpopulations of T lymphocytes.
authors.affiliation: Department of Parasitology, Kaohsiung Medical College, Taiwan, Republic of China.
authors.forename: J D
authors.initials: JD
authors.lastname: Lee
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
authors.forename: J H
authors.initials: JH
authors.lastname: Chang
authors.forename: L Y
authors.initials: LY
authors.lastname: Chung
authors.forename: E R
authors.initials: ER
authors.lastname: Chen
authors.forename: C M
authors.initials: CM
authors.lastname: Yen
mesh_terms: D019264:Adoptive Transfer; D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D001921:Brain / Q000469:parasitology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017206:Strongylida Infections / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth