title: L-NA-sensitive rCBF augmentation during vibrissal stimulation in type III nitric oxide synthase mutant mice.
abstract: Regional cerebral blood flow (rCBF) was studied in type III nitric oxide (NO) synthase (endothelial, eNOS) mutant and wild type mice during mechanical whisker stimulation before and after nitro-L-arginine (L-NA) superfusion using the closed cranial window technique. rCBF increased equally in cortical barrel fields in both strains during stimulation, as measured by laser Doppler-flowmetry, and was inhibited by L-NA superfusion (1 mM) in both groups. Hence, coupling of blood flow and metabolism appears neuronal NOS-(nNOS) but not eNOS-dependent in cortical barrel fields of the mouse.
authors.affiliation: Department of Neurosurgery and Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.
authors.forename: C
authors.initials: C
authors.lastname: Ayata
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: W
authors.initials: W
authors.lastname: Meng
authors.forename: P
authors.initials: P
authors.lastname: Huang
authors.forename: M A
authors.initials: MA
authors.lastname: Moskowitz
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D007527:Isoenzymes / Q000235:genetics*; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D009154:Mutation; D019001:Nitric Oxide Synthase / Q000235:genetics*; D019335:Nitroarginine / Q000494:pharmacology*; D010812:Physical Stimulation; D014738:Vibrissae / Q000502:physiology*
chemical_list: D007527:Isoenzymes; D019335:Nitroarginine; D019001:Nitric Oxide Synthase