title: Enrichment of benzo[a]pyrene in smoked food products and determination by high-performance liquid chromatography-fluorescence detection.
abstract: We developed a procedure for trace enrichment of benzo[a]pyrene (BP) in extracts of smoked food products, and an HPLC-fluorescence detection (FL) method for determination of BP in the enriched extracts. The procedure consists in extraction/sonication of the lyophilized product in hexane, clean-up of the hexane extract by passage through a Sep-Pak Silica Plus cartridge and, subsequently, by partitioning between hexane and dimethyl sulphoxide, and concentration of the BP using a Sep-Pak C18 Plus cartridge. HPLC-FL and quantification limits were 0.049 microgram/l in acetonitrile (< 0.0067 microgram/kg of smoked food) and 0.089 microgram/l in acetonitrile (< 0.012 microgram/kg), respectively. Recovery (94.1%) and RSD (< 8.65%) were satisfactory. When applied to 15 types of sausage, mean BP content was 0.022 microgram/kg, and all but two samples (both treated with wood smoke) had BP contents below the 0.03 microgram/kg limit imposed in EU legislation for smoking-flavour agents.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, Spain.
authors.forename: M S
authors.initials: MS
authors.lastname: García Falcón
authors.forename: S
authors.initials: S
authors.lastname: González Amigo
authors.forename: M A
authors.initials: MA
authors.lastname: Lage Yusty
authors.forename: M J
authors.initials: MJ
authors.lastname: López de Alda Villaizán
authors.forename: J
authors.initials: J
authors.lastname: Simal Lozano
mesh_terms: D001564:Benzo(a)pyrene / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005511:Food Handling; D012906:Smoke; D013050:Spectrometry, Fluorescence
chemical_list: D012906:Smoke; D001564:Benzo(a)pyrene