[Two strains of genetically hypertensive rats, LH and SRH, have distinct profiles of postnatal expression of tropoelastin and type III collagen in the aortic wall].
Experimental pharmacology and studies on hypertension frequently use genetically hypertensive animal models like the SHR or the Lyon hypertensive rat LH. In order to further characterize these two models we measured the expression levels of three major extracellular matrix components in the aortic wall, tropoelastin (TE) and type I and type III collagen, during postnatal development. The type I collagen expression decreases progressively during the first twelve weeks of postnatal development without significant differences between SHR and LH, or their normotensive controls, WKY or LN respectively. No differences were detected either for the expression levels of TE and type III collagen between the hypertensive strains and their respective controls. However, direct comparison of the two hypertensive strains SHR and LH, revealed a specific, strong increase of TE and type III expression for the LH at 5 and 12 weeks (p < 0.001 and 0.005 respectively). The evolution of the ratios of expression levels between the two collagens (type III/type I) on one side and of TE and collagen type I (TE/type I) on the other side were similar for the hypertensive strains and their respective controls, but diverged significantly for LH and SHR animals (up to p < 0.001 depending on the age group). Both indicators, III/I and TE/I, are considerably higher in LH compared to SHR from 5 weeks of postnatal development onwards. Our results indicate that the genes for TE and type I and III collagen are regulated during postnatal development of LH and SHR. It is however not possible at this point to establish a link between mRNA levels and hypertension in these animals. Nevertheless, the ratios III/I and TE/I seem to be good phenotypic markers for the characterisation of LH and SHR strains.
Holzenberger
M
M
Institut d'embryologie cellulaire et moléculaire, CNRS UMR 9924 et Collège de France, Nogent-sur-Marne.
Dumanois-Le Tan
V
V
Ayer-Lelièvre
C
C
Vincent
M
M
Safar
M
M
Renaud
J F
JF
D000818:Animals; D001011:Aorta / Q000254:growth & development / Q000378:metabolism; D003094:Collagen / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D006973:Hypertension / Q000235:genetics*; D017403:In Situ Hybridization; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D014333:Tropoelastin / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D014333:Tropoelastin; D003094:Collagen
Deux souches de rats génétiquement hypertendues, LH et SHR, se différencient par leur profil postnatal d'expression de tropoélastine et de collagène de type III dans la paroi aortique.