Experimental evidence for structure-activity features in common between mammalian histidine decarboxylase and ornithine decarboxylase.
Common protein motifs between histidine decarboxylase (HDC) and ornithine decarboxylase (ODC) were detected by computational analysis. Mutants were generated and expressed in vitro. In both enzymes, terminal PEST-region-containing fragments are not essential for decarboxylation (PEST regions are sequence fragments enriched in proline, glutamic acid, serine and threonine residues in a hydrophilic fragment flanked by cationic amino acids). The substitution of a very well conserved histidine residue by alanine causes a severalfold increase of the apparent K(m) values for the respective substrates.
Engel
N
N
Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Olmo
M T
MT
Coleman
C S
CS
Medina
M A
MA
Pegg
A E
AE
Sánchez-Jiménez
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017931:DNA Primers; D005333:Fetus; D006640:Histidine Decarboxylase / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008322:Mammals; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion
D017931:DNA Primers; D011994:Recombinant Proteins; D009955:Ornithine Decarboxylase; D006640:Histidine Decarboxylase