title: Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV.
abstract: The nucleotide sequence of a 26.7 kb DNA segment from the left arm of Saccharomyces cerevisiae chromosome IV is presented. An analysis of this segment revealed 11 open reading frames (ORFs) longer than 300 bp and one split gene. These ORFs include the genes encoding the large subunit of RNA polymerase II, the biotin apo-protein ligase, an ADP-ribosylation factor (ARF 2), the 'L35'-ribosomal protein, a rho GDP dissociation factor, and the sequence encoding the protein phosphatase 2A. Further sequence analysis revealed a short ORF encoding the ribosomal protein YL41B, an intron in a 5' untranslated region and an extended homology with another cosmid (X83276) located on the same chromosome. The potential biological relevance of these findings is discussed.
authors.affiliation: Hans-Knöll-Institut für Naturstoff-Forschung, Department of Cell and Molecular Biology, Jena, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wölfl
authors.forename: V
authors.initials: V
authors.lastname: Hanemann
authors.forename: H P
authors.initials: HP
authors.lastname: Saluz
mesh_terms: D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D019731:Carbon-Nitrogen Ligases; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids / Q000032:analysis / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis*; D001330:Electronic Data Processing; D019204:GTP-Binding Proteins / Q000235:genetics; D007438:Introns; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D054648:Protein Phosphatase 2; D012319:RNA Polymerase II / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D020668:rap GTP-Binding Proteins
chemical_list: D004271:DNA, Fungal; C489280:RPL35A protein, S cerevisiae; C472563:RPL41 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; D020668:rap GTP-Binding Proteins; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C027739:biotin-(acetyl-CoA carboxylase)synthetase