title: Adherence of Aeromonas caviae to human cell lines Hep-2 and Caco-2.
abstract: Adherence of Aeromonas caviae to HEp-2 and Caco-2 cell monolayers was investigated with 24 clinical isolates. Growth phase, temperature, multiplicity of infection and length of incubation affected adherence. Treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin B and cycloheximide to the monolayer significantly reduced the adherence capabilities of the strains investigated. The use of chloramphenicol to inhibit protein synthesis reduced the adhesive capabilities of bacteria grown in liquid medium and those subjected to mechanical shearing. Light microscopy, scanning and transmission electron microscopy were employed in the investigation of bacteria-bacteria and bacteria-monolayer interactions and indicated similarities with the aggregative adherence patterns of the Enterobacteriaceae. The presence of extracellular bacterial appendages and their correlation with increased adhesive capacity may indicate a role in the process of adherence.
authors.affiliation: Department of Medical Microbiology, University of Sheffield Medical School.
authors.forename: J P
authors.initials: JP
authors.lastname: Thornley
authors.forename: J G
authors.initials: JG
authors.lastname: Shaw
authors.forename: I A
authors.initials: IA
authors.lastname: Gryllos
authors.forename: A
authors.initials: A
authors.lastname: Eley
mesh_terms: D000333:Aeromonas / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D018938:Caco-2 Cells / Q000382:microbiology*; D002294:Carcinoma, Squamous Cell / Q000382:microbiology* / Q000473:pathology; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D008934:Mitogens / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013314:Stress, Mechanical; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D008934:Mitogens; D011500:Protein Synthesis Inhibitors; D010504:Periodic Acid; D003571:Cytochalasin B; D002701:Chloramphenicol; D003513:Cycloheximide; C009288:metaperiodate; D014357:Trypsin