title: DNA-aneuploidy as marker for neoplasia in G1-urothelial carcinomas.
abstract: Biopsies from 60 grade-1 urothelial carcinomas and 50 normal bladder mucosae have been investigated by DNA-image-cytometry after enzymatic cell separation to determine the diagnostic sensitivity of DNA-aneuploidy for the identification of low grade urothelial neoplasia. Two different modes to detect DNA-aneuploidy were compared: the conventional application of a threshold at 2.2c for the modal DNA value and the use of the Kolmogoroff-Smirnow (KS) test to compare GO/1 phase fractions of the cells under analysis and the internal reference cells. Whereas the specificity for both types of analysis was 100%, the sentitivity of the latter method was 73.3% compared to 48.3% of the conventional procedure. Therefore, the application of the KS-test for the identification of DNA- stemlines as aneuploid results in an increased detection rate was compared with the conventional threshold procedure. The high prevalence of DNA- stemline aneuploidy even in low grade urothelial cancers may qualify this marker as a possible aid in the cytologic evaluation of urine and bladder washings.
authors.affiliation: Department of Urology, Technical University of Aachen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Planz
authors.forename: E
authors.initials: E
authors.lastname: Striepecke
authors.forename: J M
authors.initials: JM
authors.lastname: Wolff
authors.forename: P
authors.initials: P
authors.lastname: Effert
authors.forename: G
authors.initials: G
authors.lastname: Jakse
authors.forename: A
authors.initials: A
authors.lastname: Böcking
mesh_terms: D000782:Aneuploidy; D014408:Biomarkers, Tumor / Q000235:genetics*; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D019044:Image Cytometry; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers