Diminished antioxidant defense potential of liver, erythrocytes and serum from rats with subacute methanol intoxication.
The activities of superoxide dismutase (SOD), catalase glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R) and the concentration of ascorbate, alpha-tocopherol, non-protein and protein-bound sulfhydryl compounds, and thiobarbituric acid-reactive substances (TBA-rs) in liver, erythrocytes and serum of rats dosed with 1.5 g methanol/kg bw were measured after 6, 12 and 24 h and 2, 5 and 7 d. Hematological erythrocyte parameters were also determined. Liver GSH-Px and GSSG-R activities, SH-groups and ascorbate were significantly diminished at 12 and 24 h, while TBA-rs increased. Blood SOD, GSH-Px and GSSG-R activities and sulfhydryl-group concentrations were reduced while TBA-rs were elevated. Methanol given to rats impaired liver, erythrocyte and blood serum antioxidant mechanisms.
Skrzydlewska
E
E
Department of Analytical Chemistry, Medical Academy, Bialystok, Poland.
Farbiszewski
R
R
D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D002374:Catalase / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D000432:Methanol / Q000506:poisoning* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D001205:Ascorbic Acid; D000432:Methanol