Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase.
The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.
Duque
H
H
Institute for Molecular Virology, University of Wisconsin-Madison 53706, USA.
Palmenberg
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AC
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D003429:Cross Reactions; D012321:DNA-Directed RNA Polymerases / Q000276:immunology*; D018604:Epitope Mapping; D006367:HeLa Cells; D006801:Humans; D008574:Mengovirus / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D012321:DNA-Directed RNA Polymerases