Possible mechanisms for an irregular vessel coagulation when long laser pulses are used in the treatment of port-wine stains.
The laser treatment of port-wine stains (PWS) has as a main aim the irreversible damage of ectatic vessels. Blood content of the subcutaneous venous plexus in PWS can be increased by a factor of seven or more, compared to that of the normal skin. The venous blood velocity ranges from 0.1-1 mm/s in capillaries to approximately 22 mm/s in larger vessels of about 300 microns in diameter. A PWS, selected for study, was irradiated with a multiline argon laser 488/515 nm wavelength, 1.5 W power, 200 ms pulse duration, 0.5 mm beam diameter and repetition frequency up to 5 Hz. Laser shots were placed adjacently in an area of 1 cm2. Using these parameters, in the case of dilated PWS vessels with an optical penetration depth and thermal diffusion length less than the diameter of the vessel, together with a transit time of blood across the irradiated spot less than the pulse duration, and estimating that during a pulsed laser emission of 200 ms, the blood has travelled a distance of 3-4 mm, there is a strong indication that hemodynamics during irradiation may influence the pattern of coagulation and agglutination. Thrombosis should occur in the case of small vessels and, in larger vessels, the coagulated blood will only partly fill the lumen. The structure developed in the vessel interior may also change continuously with time, as the coagulated material is progressively replaced by fibrotic tissue and the irregular agglutination pattern may be due to inhomogeneity in the absorbed optical energy.
Trelles
M A
MA
Instituto Médico Vilafortuny, Tarragona, Spain.
Svaasand
L O
LO
Vélez
M
M
Trelles
K
K
Fernández
R
R
Verkruysse
W
W
D055592:Biophysical Phenomena; D001703:Biophysics; D001777:Blood Coagulation; D001783:Blood Flow Velocity; D002196:Capillaries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D005355:Fibrosis; D006384:Hemagglutination; D006439:Hemodynamics; D006801:Humans; D017075:Laser Coagulation / Q000009:adverse effects / Q000379:methods*; D008297:Male; D019339:Port-Wine Stain / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D013927:Thrombosis; D013997:Time Factors