Molecular cloning of the mitogenic mannose/maltose-specific rhizome lectin from Calystegia sepium.
cDNA clones encoding the mitogenic mannose/maltose-specific lectin from the rhizomes of hedge bindweed (Calystegia sepium) have been isolated and sequenced. Comparison of the deduced amino acid sequence and the molecular weight of the lectin subunit as determined by mass spectrometry indicated that the mature protein comprises the entire open reading frame of the cDNA, which implies that the primary translation product contains no signal peptide and is not proteolytically processed. Searches in the databases revealed sequence homology with the previously described lectins from the taxonomically unrelated Moraceae species Artocarpus integrifolia and Maclura pomifera.
Van Damme
E J
EJ
Laboratorium voor Fytopathologie en Plantenbescherming, Katholieke Universiteit Leuven, Belgium.
Barre
A
A
Verhaert
P
P
Rougé
P
P
Peumans
W J
WJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007526:Isoelectric Point; D037102:Lectins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment
D000596:Amino Acids; D018076:DNA, Complementary; D037102:Lectins; D037121:Plant Lectins; D012333:RNA, Messenger; D018749:RNA, Plant; C043696:jacalin; C034551:maclurin