title: Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide.
abstract: A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.
authors.affiliation: Department of Medical Anatomy, Panum Institute, University of Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Röpke
authors.forename: J
authors.initials: J
authors.lastname: Hald
authors.forename: P
authors.initials: P
authors.lastname: Guldberg
authors.forename: J
authors.initials: J
authors.lastname: Zeuthen
authors.forename: L
authors.initials: L
authors.lastname: Nørgaard
authors.forename: L
authors.initials: L
authors.lastname: Fugger
authors.forename: A
authors.initials: A
authors.lastname: Svejgaard
authors.forename: S
authors.initials: S
authors.lastname: Van der Burg
authors.forename: H W
authors.initials: HW
authors.lastname: Nijman
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
authors.forename: M H
authors.initials: MH
authors.lastname: Claesson
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D015789:HLA-A2 Antigen / Q000276:immunology; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D009154:Mutation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000276:immunology*
chemical_list: D015789:HLA-A2 Antigen; D016159:Tumor Suppressor Protein p53