Fusion between Jurkat cell and PEO-lipid modified liposome.
Direct fusion between Jurkat cell and a liposome modified with poly(ethylene oxide)-bearing lipid (PEO-lipid) was examined using diphtheria toxin fragment A (DTA) as the probe. Only the DTA-loaded liposome modified with PEO-lipid(n = 32) (n is the number of ethylene oxide units) exerted significant cytotoxicity against Jurkat cells, while liposomes lacking either the PEO-lipid or DTA did not. Liposomes modified by the PEO-lipid with shorter PEO chain(n = 5 or 15) did not show any cytotoxicity, irrespective of their DTA-loading. The cytotoxicity was observed even in the presence of cytochalasin B, an inhibitor of endocytosis. Judging from these results, we concluded that the PEO-lipid(n = 32)-modified liposome directly fused with plasma membrane of Jurkat cell.
Higashi
N
N
Supermolecules Project, Research Development Corporation of Japan (JRDC), Kyoto, Japan.
Yamauchi
M
M
Okumura
Y
Y
Nakanishi
M
M
Sunamoto
J
J
D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004167:Diphtheria Toxin / Q000378:metabolism / Q000633:toxicity*; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology*; D008858:Microscopy, Phase-Contrast; D015394:Molecular Structure; D010446:Peptide Fragments / Q000378:metabolism / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D014280:Triglycerides / Q000737:chemistry / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D004167:Diphtheria Toxin; D008081:Liposomes; D010446:Peptide Fragments; D014280:Triglycerides; C029795:diphtheria toxin fragment A; D003571:Cytochalasin B; D011092:Polyethylene Glycols