Sensitivity of Daudi cells to tumor necrosis factor alpha: early changes in polyphosphoinositide metabolism.
The effects of recombinant Tumor Necrosis Factor alpha (r-TNF alpha) on polyphosphoinositide metabolism were examined in a Burkitt Lymphoma cell line (Daudi cells). After 1 h of in vitro treatment with r-TNF alpha, the incorporation of 32Pi into phosphatidylinositol 4,5-phosphate (PtdInsP2), phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol (PtdIns) was reduced compared with controls, confirming previous findings observed in other cell lines of a specific PtdIns breakdown following r-TNF alpha treatment. The novelty of this study is therefore the demonstration of early changes in polyphosphoinositide metabolism during the antiproliferative response elicited by this cytokine in Daudi cells.
Santavenere
E
E
Istituto di Morfologia Umana Normale, Chieti, Italy.
Di Pietro
R
R
Di Domenicantonio
L
L
Rana
R
R
D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C037178:phosphatidylinositol 4-phosphate