Quantitative conversion of glucose into glucose 6-phosphate by intact Escherichia coli cells.
The use of intact Escherichia coli cells for the conversion of glucose into glucose 6-phosphate using the Uhp system for transport of the phosphorylated sugar out of the cell was investigated. The strain E. coli DF214, which is not capable of glucose 6-phosphate catabolism via glycolysis or the Entner-Douderoff pathway, was used. The efflux of glucose 6-phosphate was dependent on the presence of UhpT, the hexose-phosphate transporter, plus the presence of the uncoupler carbonyl cyanide m-chlorophenylhydrazone or the ionophore valinomycin. At low glucose concentrations (e.g. 2.5 mM), near-quantitative conversion (> or = 80%) of glucose into extracellular glucose 6-phosphate can be achieved. When cells are incubated for a shorter period of time, complete conversion can occur.
van der Zee
J R
JR
Department of Microbiology, E.C. Slater Institute, BioCentrum, University of Amsterdam, The Netherlands.
Postma
P W
PW
Hellingwerf
K J
KJ
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009154:Mutation
D009004:Monosaccharide Transport Proteins; D019298:Glucose-6-Phosphate; D005947:Glucose