Molecular cloning and mapping of a human cDNA (SC5DL) encoding a protein homologous to fungal sterol-C5-desaturase.
We have isolated a human cDNA clone homologous to fungal ERG3, a gene encoding sterol C-5 desaturase. The full nucleotide sequence of this human cDNA revealed a 708-bp open reading frame that encodes a 236-amino-acid polypeptide. The gene was expressed in all normal human tissues examined. We determined its location to chromosome 11q23.3 by fluorescence in situ hybridization.
Matsushima
M
M
Laboratory of Molecular Medicine, University of Tokyo, Japan.
Inazawa
J
J
Takahashi
E
E
Suzumori
K
K
Nakamura
Y
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D015152:Blotting, Northern; D002175:Candida / Q000201:enzymology; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010088:Oxidoreductases; C039306:sterol delta-5 desaturase