title: Induction and postranslational expression of G-CSF and RANTES in a first trimester trophoblast cell line by lipopolysaccharide.
abstract: OBJECTIVE
  Comparatively little is known about the capacity of first trimester trophoblasts to respond to an infection and coordinate an immune response. This study characterizes the LPS induction of G-CSF and RANTES in a first trimester trophoblast cell line.
  
  
  METHODS
  HTR-8/SV neo cells were exposed to LPS (1 micrograms/ml) for 0, 2, 4, 6, 8, and 24 hours in DMEM-Ham's F-12 media supplemented with 10% fetal bovine serum. Cytokine levels in culture supernatants were measured by ELISA. Northern analysis of total RNA was conducted using antisense cytokine probes.
  
  
  RESULTS
  Levels of immunoreactive G-CSF and RANTES from LPS induced cultures at 24 hours were 10-fold and 8.5-fold greater than cytokine levels from non-induced cells at 24 hours, respectively (P < 0.01). Under LPS induction, maximal rates of G-CSF and RANTES transcription occurred at 24 hours and 8 hours, respectively.
  
  
  CONCLUSIONS
  The LPS induction of proinflammatory cytokines in a first trimester trophoblast cell line supports the contention that first trimester trophoblasts participate in cytokine based immune signaling in response to infection.
authors.affiliation: Department of Obstetrics and Gynecology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Svinarich
authors.forename: O M
authors.initials: OM
authors.lastname: Bitonti
authors.forename: H
authors.initials: H
authors.lastname: Araneda
authors.forename: R
authors.initials: R
authors.lastname: Romero
authors.forename: B
authors.initials: B
authors.lastname: Gonik
mesh_terms: D015152:Blotting, Northern; D002460:Cell Line / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D012313:RNA; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D018946:Chemokine CCL5; D008070:Lipopolysaccharides; D016179:Granulocyte Colony-Stimulating Factor; D012313:RNA