title: Canine leucocyte histamine release: response to antigen and to anti-IgE.
abstract: Histamine release from canine leucocyte-enriched peripheral blood preparations was measured in atopic, non-atopic and artificially sensitised dogs after immunological challenge with D. farinae antigen and anti-IgE. Total cell histamine and spontaneous histamine release was also measured. The total cell histamine content of equal leucocyte preparations was not statistically significant between the atopic and non-atopic groups. At all dilutions of antigen a higher amount of histamine was released from the leucocytes of atopic dogs than was seen in the non-atopic group. No histamine release in response to D. farinae was seen in the sensitised dogs although a statistically significant increase in serum D. farinae-specific IgE could be demonstrated after sensitisation (P < 0.03). Histamine release in response to anti-IgE was significantly greater in the atopic dogs than the non-atopic dogs (P < 0.004) and the sensitised dogs (P < 0.003). There was no statistically significant difference in total serum IgE between the groups. The authors conclude that the leucocytes of atopic dogs have a greater tendency to release histamine than those of normal and artificially sensitised dogs and that this is independent of the concentration of total serum IgE or antigen-specific IgE. They suggest that there may be immunoregulatory abnormalities in atopic dogs intrinsic to the atopic state as is described in man.
authors.affiliation: University of Edinburgh, Department of Veterinary Clinical Studies, Roslin, Midlothian, UK.
authors.forename: H A
authors.initials: HA
authors.lastname: Jackson
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
authors.forename: R E
authors.initials: RE
authors.lastname: Halliwell
mesh_terms: D000485:Allergens / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000941:Antigens / Q000494:pharmacology*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary; D004285:Dogs; D005260:Female; D006632:Histamine / Q000097:blood; D006636:Histamine Release; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008925:Mites / Q000276:immunology
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D006632:Histamine