title: Characterization and quantitation of the apoproteins of high-density lipoprotein by capillary electrophoresis.
abstract: A method has been developed using capillary electrophoresis (CE) to quantitate plasma levels of apoprotein A-I (apoA-I) and apoprotein A-II (apoA-II) in high-density lipoprotein (HDL) samples. ApoA-I and apoA-II are resolved by CE in delipidated and non-delipidated HDL samples. Concentrations of apoA-I and apoA-II were calculated from their peak areas in the electropherogram. Results of the analysis of Sigma plasma standards (Controls 1 and 2) using CE are in good agreement with values obtained by Sigma using immunoturbidimetric assay. CE and reverse-phase high-performance liquid chromatography (RP-HPLC) were found to be complementary in the study of apoA-I and apoA-II. RP-HPLC resolves the isoforms of purified apoA-I and apoA-II, but it cannot resolve mixtures of them because the retention times of the isoforms overlap. CE separates apoA-I from apoA-II, but it does not resolve the isoforms. Matrix-assisted laser desorption/ ionization mass spectrometry was used to identify the isoforms of apoA-I and apoA-II by their molecular weight (M(r)) in fractions collected from RP-HPLC.
authors.affiliation: Department of Chemistry, Texas A & M University, College Station 77843-3255, USA.
authors.forename: I D
authors.initials: ID
authors.lastname: Cruzado
authors.forename: S
authors.initials: S
authors.lastname: Song
authors.forename: S F
authors.initials: SF
authors.lastname: Crouse
authors.forename: B C
authors.initials: BC
authors.lastname: O'Brien
authors.forename: R D
authors.initials: RD
authors.lastname: Macfarlane
mesh_terms: D001059:Apoproteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry*; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001059:Apoproteins; D008075:Lipoproteins, HDL