The VP3 gene of human group C rotavirus.
The complete nucleotide sequence of genome segment 4 from the human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequences of the genome termini with the consensus 5' and 3' terminal non-coding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequence motifs. Human group C rotavirus genome segment 4 is 2,166bp long and encodes a single open reading frame of 2,082 nucleotides (693 amino acids) starting at nucleotide 55 and terminating at nucleotide 2,136 giving a 3' untranslated region of 30 nucleotides. Alignment with the porcine group C VP3 equivalent gene showed the human gene is one amino acid longer, and that the proteins have 84.1% amino acid sequence identity. A conserved potential nucleotide binding motif shared with the porcine VP3 sequence was identified. Analogy with the group A rotaviruses suggested that the genome segment 4 encodes the group C rotavirus guanylyltransferase.
Samarbaf-Zadeh
A R
AR
University Medical School, Southampton General Hospital, United Kingdom.
Lambden
P R
PR
Green
S M
SM
Deng
Y
Y
Caul
E O
EO
Clarke
I N
IN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins; D004279:DNA, Viral; C442546:VP3 protein, Rotavirus