Distinct, very high frequency oscillations in the activity and amount of active isozyme of lactate dehydrogenase in murine erythroleukaemic cells and a cell-free system.
Electrophoretic and kinetic determinations of the activity of lactate dehydrogenase in murine erythroleukaemic (MEL) cells, sampled at 1 min intervals, reveal distinct oscillations in the activity and amount of active isozyme. Both oscillations have periods in the range of 2-6 min (probably less) and both appear to be rhythmically modulated with respect to period, amplitude and mean. The oscillations also occur in cell-free systems, a fact which throws doubt on the value of studies where it is assumed that such preparations have constant composition.
Ferreira
G M
GM
Department of Medical Biochemistry, Medical School, University of the Witwatersrand, Parktown, Johannesburg, South Africa.
Hammond
K D
KD
Gilbert
D A
DA
D000204:Activity Cycles; D000818:Animals; D002474:Cell-Free System; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D051379:Mice; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase