[Structural and functional analysis of the human immunodeficiency glycoprotein gp120 with homologous synthetic peptides].
The purpose of the study was to identify sites of gp 120, which are responsible for CD4 binding and induce tumor necrosis factor-alpha (TNF-alpha) synthesis. Seven peptides were synthesized from gp 120. The peptides were studied in the following biological tests: binding to CD4 molecules of the Jurkat cell clones 3G6 and PBMC of healthy persons. There was TNF-alpha induction in healthy and HIV-positive individuals and its correlation was found with p24 antigenemia in HIV patients. The peptides 420-440, 426-452, 369-384, 255-272 bind to T-lymphocytic CD4 and induced TNF-alpha. The peptide 436-451 binds to CD4, but failed to induced TNF-alpha, which suggests that the latter may be used as a basis for HIV-infection vaccine.
Ketlinskiĭ
S A
SA
Kalinina
N M
NM
Prusakov
A N
AN
Kaurov
O A
OA
D015704:CD4 Antigens / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000502:physiology*; D015658:HIV Infections / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D011485:Protein Binding; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D014409:Tumor Necrosis Factor-alpha
Strukturno-funktsional'nyĭ analiz glikoproteina gp120 virusa immunodefitsita cheloveka s pomoshch'iu sinteticheskikh peptidov, gomologichnykh ego strukture.