Type D retrovirus markers in healthy Africans from Guinea.
Sixteen matching sera and DNA samples from healthy African blood donors living in rural areas of Guinea were analysed for the presence of type D retrovirus markers. Screening for the antibodies against structural proteins of Mason-Pfizer monkey virus (M-PMV) was carried out by Western blot with a purified M-PMV as an antigen. Eight out of 16 sera samples were found to contain antibodies against at least two gag gene-coded proteins, and three of these were weakly positive against env gene-coded protein. Using PCR amplification and Southern hybridization, we detected M-PMV-like gag sequences in 11 out of 16 samples and env-related sequences in 8 out of 16 samples. Six DNAs were found to contain both M-PMV gag- and env-related sequences. Restriction endonuclease analysis of the PCR-amplified gag sequences from two individuals and direct DNA sequencing analysis of the amplimers confirmed their M-PMV-like origin. Detection of antibodies and M-PMV-related sequences in blood donors from Guinea, but not in French or Algerian blood donors, indicated exogenous SRV infection in humans from certain geographic areas of Western Africa.
Morozov
V A
VA
Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research and Development Center, MD 21702, USA.
Lagaye
S
S
Lyakh
L
L
ter Meulen
J
J
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D015415:Biomarkers; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D002648:Child; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016701:Guinea; D006801:Humans; D008297:Male; D016093:Mason-Pfizer monkey virus / Q000302:isolation & purification*; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D012192:Retroviridae Infections / Q000821:virology; D014412:Tumor Virus Infections / Q000821:virology; D015678:Viral Structural Proteins / Q000032:analysis
D000914:Antibodies, Viral; D015415:Biomarkers; D004279:DNA, Viral; D015678:Viral Structural Proteins; D004262:DNA Restriction Enzymes