Production of ouabain by rat adrenocortical cells.
Our aim was to identify which adrenocortical cells produce ouabain and how it is regulated in vitro. With the help of an ouabain radioimmunoassay developed in our laboratory, we found that ouabain is produced both by zona glomerulosa and fasciculata cells. Our results were confirmed by reverse-phase HPLC. ACTH increased ouabain production in both cell types. Angiotensin-II, as well as changes in the potassium concentration of the incubation medium, affected ouabain production only in the zona glomerulosa.
Beck
M
M
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
Szalay
K S
KS
Nagy
G M
GM
Tóth
M
M
de Châtel
R
R
D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D010042:Ouabain / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D015383:Zona Fasciculata / Q000187:drug effects / Q000378:metabolism; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism
D000804:Angiotensin II; D010042:Ouabain; D000324:Adrenocorticotropic Hormone; D011188:Potassium