Expression of nicotinic acetylcholine receptor mRNA in the adult rat peripheral vestibular system.
The mRNA expression of the neuronal nicotinic acetylcholine receptor subunits was determined in adult rat vestibular end-organs and in Scarpa's ganglion (SCG) by in situ hybridization with [35S] riboprobes. Neurons in the SCG expressed the alpha 4-7 and beta 2-3 mRNAs, but not alpha 3 or beta 4 mRNAs. Not all SCG neurons expressed every mRNA found in SCG. The alpha 6 and beta 2-3 riboprobes labeled all neurons, but alpha 4, alpha 5, and alpha 7 mRNAs were selectively expressed in one or more subpopulations of SCG neurons. Vestibular sensory hair cells, in contrast, expressed only alpha 9 mRNA.
Hiel
H
H
Neurochemistry Laboratory, Boys Town National Research Hospital, Omaha, NE 68131, USA.
Elgoyhen
A B
AB
Drescher
D G
DG
Morley
B J
BJ
D000818:Animals; D005260:Female; D018069:Hair Cells, Vestibular / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*; D014725:Vestibular Nerve / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000378:metabolism*
D012333:RNA, Messenger; D011978:Receptors, Nicotinic