Purification of protein C a natural anticoagulant, from human plasma by affinity chromatography with Convanavalin A.
We have developed a rapid, efficient, and inexpensive method to purify protein C from plasma using Concanavalin A conjugated to Sepharose. With this method we have obtained protein C purified to homogeneity, as measured in polyacrylamide gels. We discuss each of the steps of our method and give the results of the purification.
Pérez-Campos
E
E
Biochemistry and Immunology Unit, Technological Institute of Oaxaca, Mexico.
Córdoba
F
F
Pérez-Ortega
E
E
Martínez
M
M
Zenteno
E
E
D000925:Anticoagulants / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011486:Protein C / Q000302:isolation & purification*
D000925:Anticoagulants; D011486:Protein C; D003208:Concanavalin A