Analysis of combinatorial libraries using electrospray Fourier transform ion cyclotron resonance mass spectrometry.
Electrospray ionization coupled with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry has been used to provide information about complete combinatorial libraries of small peptides containing 10(3)-10(4) components. The fidelity of attempted synthesis steps can be ascertained rapidly, and, when the extremely high resolution FTICR mass spectra are combined with appropriate computer simulation, both diversity and degeneracy of the libraries as synthesized can be assessed.
Nawrocki
J P
JP
Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
Wigger
M
M
Watson
C H
CH
Hayes
T W
TW
Senko
M W
MW
Benner
S A
SA
Eyler
J R
JR
D003198:Computer Simulation; D017357:Cyclotrons; D004574:Electromagnetic Fields; D005583:Fourier Analysis; D015698:Genomic Library; D013058:Mass Spectrometry / Q000295:instrumentation*; D010455:Peptides / Q000032:analysis*
D010455:Peptides