Ca(2+)-induced inhibition of 45Ca2+ influx and Ca2+ current in smooth muscle of the rat vas deferens.
The present study investigates how changes in intracellular Ca2+ concentration modulate the influx of 45Ca2+ in isolated rat vasa deferentia. Raising extracellular K+ concentration ([K+]0) to > or = 32 mM increased 45Ca2+ influx during the 1st min in solutions containing 0.03-1.5 mM extracellular Ca2+ concentration ([Ca2+]0). During the 6th min in [K+]0 > or = 50 mM, 45Ca2+ influx was less than during the 1st min. This decline in 45Ca2+ influx occurred for [Ca2+]0 > or = 0.4 mM. Procaine potentiated K(+)-stimulated 45Ca2+ influx in 1.5 mM [Ca2+]0 and eliminated the decline of 45Ca2+ influx in low [Ca2-]0. Ryanodine and norepinephrine reduced K(+)-stimulated 45Ca2+ influx. 45Ca2+ content changed with time in accordance with the changes observed in 45Ca2+ influx. In isolated cells, voltage-dependent inward currents inactivated more rapidly with 1.5 mM Ca2+ as the charge carrier than with 1.5 mM Ba2+, and the steady-state inactivation relationship was shifted in the hyperpolarizing direction. Inward current was reduced with either caffeine, ryanodine, or norepinephrine. The inhibitory effects of norepinephrine were abolished by depletion of intracellular Ca2+ stores. These results are compatible with the hypothesis that K(+)-stimulated 45Ca2+ influx declines with time due to Ca(2+)-induced inhibition of Ca2- channels. Ca(2+)- and inositol 1,4,5-trisphosphate-induced releases of Ca2+ from the sarcoplasmic reticulum appear to play an important role in this process.
Khoyi
M A
MA
Department of Pharmacology, University of Nevada School of Medicine, Reno 89557-0046, USA.
Ishikawa
T
T
Keef
K D
KD
Westfall
D P
DP
D000818:Animals; D001464:Barium / Q000502:physiology; D002118:Calcium / Q000494:pharmacology* / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D002132:Calcium Radioisotopes; D004594:Electrophysiology; D004789:Enzyme Activation; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D011343:Procaine / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D012433:Ryanodine / Q000494:pharmacology; D014649:Vas Deferens / Q000187:drug effects* / Q000502:physiology*
D015220:Calcium Channels; D002132:Calcium Radioisotopes; D012433:Ryanodine; D001464:Barium; D011343:Procaine; D011493:Protein Kinase C; D011188:Potassium; D002118:Calcium