Agonist-stimulated calcium transients in PC12 cells are affected differentially by cadmium and nickel.
Rat pheochromocytoma (PC12) cells were loaded with the fluorescent indicator Fura-2 and the effects of cadmium and nickel on the mobilization of calcium elicited by bradykinin and external ATP were studied. Cadmium and nickel ions provoked a concentration-dependent decrease of the initial peak and/or the subsequent plateau phase of bradykinin-induced Ca2+ transients in a different manner: whereas cadmium (0.5-2.5 microM) diminished the calcium peak without modifying the sustained plateau, nickel (25-1000 microM) only slightly lowered the peak but markedly decreased the plateau phase. In the case of ATP-stimulated calcium transients, which are without a sustained plateau, both cadmium and nickel ions decreased the peak signal. Possible consequences are discussed in terms of a disturbance of hormone-stimulated cell activation by cadmium and nickel.
Benters
J
J
Department of Biology and Chemistry, University of Bremen, Germany.
Schäfer
T
T
Beyersmann
D
D
Hechtenberg
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D016257:Fura-2; D009532:Nickel / Q000494:pharmacology*; D016716:PC12 Cells / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D002104:Cadmium; D009532:Nickel; D000255:Adenosine Triphosphate; D001920:Bradykinin; D002118:Calcium; D016257:Fura-2