Comparison of immunological effects of Fullerene C60 and raw soot from Fullerene production on alveolar macrophages and macrophage like cells in vitro.
Two carbon modifications, C60 and Raw Soot (RS) from fullerene production, were incubated with macrophages and macrophage-like cells. Their effects were compared both to DQ12 Quartz as a positive particle control known to damage BAM and HL60M, and LPS or zymosan as biological inducers of immunological reactions. C60 as well as RS are not cytotoxic as compared to DQ12. C60 seems to induce a lower chemotactic activity than RS and DQ12. Furthermore, C60 is of no or only very little influence on the formation of reactive oxygen species (ROS). RS itself is oxidatively active both with H2DFC-DA in in vitro cell culture and with KMB in a cell free incubation under the influence of light. It is not yet clear whether the in vitro effects of RS are caused by ROS formation by the cells and/or the oxidative activity due to light as indicated from the results with KMB.
Baierl
T
T
Forschungszentrum Karlsruhe, Institut für Toxikologie, Karlsruhe, Germany.
Drosselmeyer
E
E
Seidel
A
A
Hippeli
S
S
D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D002244:Carbon / Q000494:pharmacology*; D002417:Cattle; D005030:Ethylenes / Q000032:analysis; D037741:Fullerenes; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D011791:Quartz / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D005030:Ethylenes; D037741:Fullerenes; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D011791:Quartz; D002244:Carbon; D015054:Zymosan; C036216:ethylene; D007770:L-Lactate Dehydrogenase; D000118:Acetylglucosaminidase; C069837:fullerene C60