Cloning and characterization of a novel upstream untranslated exon of the mouse Fgf-1 gene.
Fibroblast growth factor 1 (FGF-1 or aFGF), is the prototype member of the heparin-binding growth factors which are capable of angiogenesis in vivo. FGF-1 has been implicated in atherosclerosis, cancer, wound repair and inflammatory autoimmune diseases. As part of an effort to understand the role of FGF-1 in the etiopathogenesis of inflammation and cancer, we have undertaken steps to isolate and characterize the mouse Fgf-1 gene. Southern blotting and sequence analysis displayed considerable conservation within the coding and upstream untranslated regions of Fgf-1 in human, mouse, hamster, rat and bovine. By using primers derived from the 5'-untranslated exon of a rat prostate-specific Fgf-1 cDNA, a 220-bp product was amplified from mouse genomic DNA via PCR. Sequence analysis of this amplicon showed that there was 80% similarity with the corresponding region of the rat FGF-cDNA sequence. Primers designed from this amplicon and the Fgf-1 coding region were used to isolate multiple overlapping genomic clones spanning the entire mouse Fgf-1 gene. Sequencing analysis of the genomic sequence upstream from this novel 5'-untranslated exon did not reveal typical TATA, CCAAT sequences. It appears that the occurrence of multiple untranslated exons for FGF-1 is a highly conserved theme for this gene across species.
Hackshaw
K V
KV
Department of Internal Medicine, Ohio State University, William H. Davis Medical Research Center, Columbus 43210, USA.
Furlow
Z M
ZM
Chiu
I M
IM
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA; D017931:DNA Primers; D005091:Exons; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D005346:Fibroblast Growth Factors; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D011467:Prostate / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors; D004247:DNA