A cost effective base-matching assay with low backgrounds.
Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification. However, its application is often hampered by high background and high cost. We have decreased the background by approximately 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.
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Theobald Smith Research Institute, Inc., The New England Regional Newborn Screening Program, Boston, MA 02130, USA. xsu@world.std.com
Mushinsky
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Comeau
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D005838:Genotype; D005914:Globins / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000191:economics* / Q000379:methods
D005914:Globins