Measurement of bioactive neuropeptides using a chromatographic immunosensor cartridge.
Neuropeptide modulation of host defenses is associated with a number of clinical conditions in which tissue injury is caused by a process termed neurogenic inflammation. Monitoring of neuropeptide activity during both disease episodes and therapy has proven difficult due to a lack of correlation between laboratory evaluations and disease activity. Although a number of assays are available for measuring the total neuropeptide content of tissue samples, few can measure the bioactive component which is the more physiologically accurate parameter. In an attempt to overcome this problem, a receptor-affinity chromatographic technique coupled with immunological detection has been developed for measuring three bioactive neuropeptides (substance P, vasoactive intestinal peptide, and calcitonin gene-related peptide) in tissue biopsy extracts. The technique involves isolation of the active neuropeptides via their ability to bind to immobilized receptors followed by measurement of the bound materials by a series of simultaneous immunoassays. The techniques compares favourably with results obtained using conventional bioassays and has the added advantage that multiple analytes can be measured in a single procedure.
Phillips
T M
TM
Immunochemistry Laboratory, George Washington University Medical Center, Washington, DC 20037, USA.
D015374:Biosensing Techniques; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D009479:Neuropeptides / Q000032:analysis*
D009479:Neuropeptides