title: Two-dimensional electrophoresis of plant proteins and standardization of gel patterns.
abstract: Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i) comigration of external standard marker proteins with resultant horizontal and vertical stripes in the gel, and (ii) deblocking with a vapor of aqueous heptafluorobutyric acid for N-acetylserine. Approximately 5000 protein spots were separated from both the five tissues of Arabidopsis and the nine tissues of rice. Over one hundred spots were electroblotted for N-terminal sequencing. Among the newly sequenced proteins, 62 were from Arabidopsis and 51 from rice.
authors.affiliation: Research Institute for Biosciences, Science University of Tokyo, Noda, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsugita
authors.forename: M
authors.initials: M
authors.lastname: Kamo
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.forename: Y
authors.initials: Y
authors.lastname: Ohki
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis*; D012015:Reference Standards
chemical_list: D010940:Plant Proteins