Direct association between the yeast Rad51 and Rad54 recombination proteins.
The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Eschericia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro. RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.
Jiang
H
H
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA. kodadek@ccwf.cc.utexas.edu
Xie
Y
Y
Houston
P
P
Stemke-Hale
K
K
Mortensen
U H
UH
Rothstein
R
R
Kodadek
T
T
D001665:Binding Sites; D015153:Blotting, Western; D004265:DNA Helicases; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005656:Fungal Proteins / Q000378:metabolism*; D051135:Rad51 Recombinase; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase / Q000378:metabolism
D004268:DNA-Binding Proteins; D000939:Epitopes; D005656:Fungal Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; D015245:Deoxyribonuclease BamHI; D001616:beta-Galactosidase; C075468:RAD54 protein, S cerevisiae; D004265:DNA Helicases; D045643:DNA Repair Enzymes