Detection of Fc receptor genes from Staphylococcus aureus and streptococci by polymerase chain reaction.
A method based upon the polymerase chain reaction (PCR) for detecting genes encoding the Fc receptors of Staphylococcus aureus and streptococci is described. Primers were designed from the nucleotide sequences of the five Fc receptor genes encoding protein A, protein G, protein H, FcRA and protein V. Amplification products corresponding in size to the protein A and protein G genes were detected in S. aureus strain Cowan 1 and Streptococcus pyogenes strain G148, respectively, as expected. Str. pyogenes strain AR1 was shown to possess the type H receptor gene. Two clinical isolates of Str. pyogenes, strains IP-28 and ES-21L, were shown to possess genes for Fc receptor types FcRA and protein G, respectively. The identification of all these products was confirmed by restriction endonuclease analysis. Amplification of protein H genes from two other clinical isolates of streptococci, MS-4 and MS-38, yielded a product larger than expected and with a different restriction fragment pattern to strain AR1, indicating a new type of Fc receptor gene. This PCR method provides a DNA-based method for the determination of Fc receptor type in S. aureus and streptococci.
Yamada
S
S
Department of Microbiology, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Yamagishi
J
J
Matsumoto
A
A
D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D008565:Membrane Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011961:Receptors, Fc / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics; D015183:Restriction Mapping; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D013291:Streptococcus / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C034284:IgG Fc-binding protein, Streptococcus; D008565:Membrane Proteins; D011961:Receptors, Fc; D017452:Receptors, IgG; D013205:Staphylococcal Protein A; C064006:protein H, Streptococcus; C077032:FcrV protein, Streptococcus