Interaction of human leukotriene C4 synthase and microsomal glutathione transferase in vivo.
Microsomal glutathione transferase (mGT) specifically binds leukotriene C4 synthase in the presence of Mg2+ ion (Söderström et al., Protein Expression and Purification (1995) 6, 352-356). To investigate if this interaction occurs in vivo we screened a human lung cDNA library with a bait vector encoding human mGT in the yeast two-hybrid system. One of the five positive clones obtained encoded leukotriene C4 synthase. This clone was expressed in two heterologous systems. The recombinant protein cross-reacted with a guinea pig antibody raised against a Keyhole limpet hemocyanin coupled synthetic peptide corresponding to amino acids 141-150 of human leukotriene C4 synthase.
Surapureddi
S
S
Department of Cell Biology, Linköping University, Sweden.
Morgenstern
R
R
Söderström
M
M
Hammarström
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase