Acute promyelocytic leukemia with t(15;16;17;19) and unusual fluorescence in situ hybridization pattern with PML and RARA probes.
We report a 58-year-old female with typical morphological and clinical features of acute promyelocytic leukemia in whom a complex translocation involving chromosomes 15, 16, 17 and 19 was detected using conventional cytogenetics and fluorescence in situ hybridization (FISH) with chromosome specific paints. RARA-PML fusion was not evident by FISH, but the RARA signal was split in 74.5% of cells. GTL-banding and FISH with probes for PML, RARA and chromosome 15 specific paint raise the possibility of PML-RARA fusion on the abnormal chromosome 19 in the complex translocation. The unusual PML-RARA fusion may be related to this patient's poor response to induction therapy with all-trans-retinoic acid.
Kamal
N R
NR
Pathology Department, King Hussein Medical Center, Amman, Jordan.
Hanson
C A
CA
Dewald
G W
GW
D002884:Chromosomes, Human, Pair 15; D002885:Chromosomes, Human, Pair 16; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics*
D015342:DNA Probes; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid