Reciprocal translocation frequency in irradiated sensitive and resistant human tumor cells in correlation with clonogenic in vitro cell survival: a possibility of tumor radiosensitivity prediction?
We have investigated the yields of radiation-induced translocations in several human tumor cell lines and in normal diploid human fibroblasts by fluorescence in situ hybridization. The translocation yields were determined with respect to chromosome no. 1 in all cell lines investigated, and moreover in chromosomes nos. 2, 4 and 9 in fibroblasts and one tumor cell line. The chromosomes were "painted' with fluorescent whole chromosome-hybridization probes. The clonogenic survival of the studied cell lines was determined by standard colony-formation assay. We observed a higher frequency of reciprocal translocations in the radiosensitive cells MCF-7 and MDA-MB-436 as compared with the radioresistant cells CaSki and normal skin fibroblasts. Thus, the results suggest a possibility to predict the intrinsic tumor radiosensitivity on the basis of reciprocal translocation yield determined in cells irradiated in vitro. The correlation was observed in spite of the trisomy no. 1 which occurred in all three investigated tumor cell lines. On the other hand, the results obtained with different chromosomes in MCF-7 cells suggest that only chromosomes with relatively low "spontaneous' translocation yields are suitable for this kind of analysis.
Rave-Fränk
M
M
Abteilung Strahlentherapie, Zentrum Radiologie, Göttingen, Germany.
Virsik-Peuckert
P
P
Schmidberger
H
H
Rodemann
H P
HP
D002470:Cell Survival / Q000528:radiation effects; D002878:Chromosomes, Human, Pair 1 / Q000528:radiation effects*; D002889:Chromosomes, Human, Pair 2 / Q000528:radiation effects*; D002894:Chromosomes, Human, Pair 4 / Q000528:radiation effects*; D002899:Chromosomes, Human, Pair 9 / Q000528:radiation effects*; D003114:Colony-Forming Units Assay; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011836:Radiation Tolerance; D014178:Translocation, Genetic / Q000528:radiation effects*; D014407:Tumor Cells, Cultured / Q000528:radiation effects*; D014410:Tumor Stem Cell Assay