Early induction of the NGFI-B/Nur77 family genes in nephritis induced by anti-glomerular basement membrane antibody.
We recently isolated a novel nuclear receptor NOR-1, which is a member of the steroid/thyroid receptor superfamily, and belongs to the NGFI-B/Nur77 family. In the present study, we examined gene expression of NOR-1 and its closely related members in nephritis induced by anti-glomerular basement membrane (GBM) antibody. The mRNA levels for NOR-1, NGFI-B and RNR-1 increased 24 h after injection of anti-GBM antibody (day 1). Gene expression of NOR-1 and NGFI-B reached maximum levels on day 3, gradually decreased thereafter and returned to control levels on day 28. RNR-1 reached a peak on day 7, and then decreased. Renal injuries were most prominent on day 7 and persisted until day 28, indicating that NOR-1, NGFI-B and RNR-1 genes are induced during the early stage of glomerulonephritis and may be associated with the progression of glomerulonephritis. The induction of the NGFI-B gene was less remarkable than that of NOR-1 and RNR-1. In addition, administration of glucocorticoid hormone suppressed NOR-1 and RNR-1 gene expression to almost normal levels, whereas NGFI-B gene expression was not significantly repressed. These findings also suggest that the NGFI-B/Nur77 family may possess different biological roles and NGFI-B might act as a general transcription factor in cell function.
Hayashi
K
K
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Ohkura
N
N
Miki
K
K
Osada
S
S
Tomino
Y
Y
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage* / Q000276:immunology; D001485:Basement Membrane / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D008297:Male; D009393:Nephritis / Q000139:chemically induced / Q000235:genetics*; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D051381:Rats; D011921:Rats, Inbred WKY; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D000906:Antibodies; D004268:DNA-Binding Proteins; C539820:Nr4a1 protein, rat; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors