title: Structural determinants within the subunit protein of Ty1 virus-like particles.
abstract: The Ty virus-like particles (VLPs) are functionally analogous to retroviral particles. They package the enzymes and the RNA necessary for retrotransposition, and mediate the integration of the reverse-transcription product into the genome of the host cell. Here we map three structural determinants of particle assembly in the subunit protein. We have also identified key residues in these regions that seem to be involved in subunit interaction and particle morphology. In particular, two point mutations in putative amphipathic helices have remarkable effects on VLP morphology, increasing the diameter as much as eightfold.
authors.affiliation: Department of Biochemistry, Oxford, UK.
authors.forename: E
authors.initials: E
authors.lastname: Martin-Rendon
authors.forename: G
authors.initials: G
authors.lastname: Marfany
authors.forename: S
authors.initials: S
authors.lastname: Wilson
authors.forename: D J
authors.initials: DJ
authors.lastname: Ferguson
authors.forename: S M
authors.initials: SM
authors.lastname: Kingsman
authors.forename: A J
authors.initials: AJ
authors.lastname: Kingsman
mesh_terms: D000595:Amino Acid Sequence; D004251:DNA Transposable Elements; D017353:Gene Deletion; D008969:Molecular Sequence Data
chemical_list: D004251:DNA Transposable Elements