Expression of the gene for the POU domain transcription factor Tst-1/Oct6 is regulated by an estrogen-dependent enhancer.
Expression of the POU domain protein Tst-1/Oct6 during development of glia and neurons is subject to a tight multifactorial control. Here we show that 17beta-estradiol increases the level of endogenous Tst-1/Oct6 in glial cells. This effect was mediated at the level of gene expression by an enhancer present in the 5' flanking region of the mouse gene for Tst-1/Oct6, approximately 5 kb upstream of the transcriptional start site. The enhancer contained as the functional element a sequence motif that closely resembled a classical estrogen response element. It consisted of an imperfect palindrome with a spacing of 3 bp, and was bound in vitro by activated estrogen receptor. Furthermore, this element was able to confer estrogen responsiveness when introduced into a heterologous promoter. In the Tst-1/Oct6 gene enhancer, a TPA response element was found in close proximity to the estrogen receptor binding site. As a consequence, TPA and estrogen activated transcription of the Tst-1/Oct6 gene in a synergistic manner.
Renner
K
K
Zentrum für Molekulare Neurobiologie, Universität Hamburg, Germany.
Sock
E
E
Bermingham
J R
JR
Wegner
M
M
D000818:Animals; D015153:Blotting, Western; D002874:Chromosome Mapping; D004357:Drug Synergism; D004742:Enhancer Elements, Genetic; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D050816:Octamer Transcription Factor-6; D012097:Repressor Proteins / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004967:Estrogens; D009419:Nerve Tissue Proteins; C494180:Pou3f1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; D004958:Estradiol; D013755:Tetradecanoylphorbol Acetate