[The heterogeneity of cell populations by the cytochemical characteristics of the cellular chromatin].
Cell heterogeneity of cytochemical characteristics of chromatin in the norm and after provocative factors in vitro (a heating at 45 degrees C, X-irradiation and joint actions of heating and X-ray irradiation) was investigated in the peripheral blood mononuclear cells of sheep and mouse, the lymphoid cell populations of the murine bone marrow, spleen and thymus. As a criterion of cytochemical heterogeneity were used the distinctions in the rate of staining of cell nuclei with DNA-specific dyes, that was registered by means of measurement of coefficient variation of the DNA-histogram basis peak, with a flow cytometry. Heterogeneity of cytochemical characteristics are dealt with showed a variability of spatial organization of interphase nuclei of single cells. The provocative factors bring about a decrease in the level of the cytochemical characteristics heterogeneity in the sheep mononuclear cells. In the murine cell populations studied no such equalizing of the marker of variability was revealed.
Rozanov
Iu M
IuM
Pereverzev
A E
AE
Kaminskaia
E V
EV
Vakhtin
Iu B
IuB
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D002843:Chromatin / Q000378:metabolism* / Q000528:radiation effects; D005434:Flow Cytometry; D006651:Histocytochemistry; D006358:Hot Temperature; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000528:radiation effects; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000528:radiation effects; D013194:Staining and Labeling / Q000379:methods; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects; D013997:Time Factors
D002843:Chromatin
Geterogennost' kletochnykh populiatsiĭ po tsitokhimicheskim kharakteristikam khromatina kletok.