title: Characterization of a flow-sorted human chromosome 10 cosmid library by FISH.
abstract: Fluoresence in situ hybridization (FISH) was used to localize cosmids to regions of human chromosome 10. A total of 301 cosmids were selected randomly from a flow-sorted human chromosome 10 cosmid library constructed from human x hamster cell line 762-8A and arrayed in microtiter storage dishes. Over 70% (211/301) of the cosmids mapped to unique regions of chromosome 10. About 7% (22/301) produced multiple hybridization signals indicative of chimeric clones or sequences repeated at low copy number. Three cosmids (3/301, or 1%) hybridized to the centromeric regions of chromosome 10 and one or more other human chromosomes. About 19% (59/301) consisted mostly or entirely of hamster DNA inserts, and about 2% (6/301) appeared to be nonrecombinants.
authors.affiliation: Department of Human and Molecular Genetics, Genome Therapeutics Corporation, Waltham, USA.
authors.forename: N S
authors.initials: NS
authors.lastname: Ma
authors.forename: C
authors.initials: C
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Benchekroun
authors.forename: L L
authors.initials: LL
authors.lastname: Deaven
authors.forename: J L
authors.initials: JL
authors.lastname: Longmire
authors.forename: D T
authors.initials: DT
authors.lastname: Moir
authors.forename: J
authors.initials: J
authors.lastname: Mao
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data
chemical_list: D015342:DNA Probes