Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum.
In previous studies (A. Marais, J. M. Bove, and J. Renaudin, J. Bacteriol. 178:862-870, 1996), we have shown that the recA gene of Spiroplasma citri R8A2 was restricted to the first 390 nucleotides of the N-terminal part. PCR amplification and sequencing studies of five additional strains of S. citri have revealed that these strains had the same organization at the recA region as the R8A2 strain. In contrast to S. citri, Spiroplasma melliferum was found to contain a full-length recA gene. However, in all five S. melliferum strains tested, a TAA stop codon was found within the N-terminal region of the recA reading frame. Our results suggest that S. melliferum, as well as S. citri, is RecA deficient. In agreement with the recA mutant genotype of S. citri and S. melliferum, we have shown that these organisms are highly sensitive to UV irradiation.
Marais
A
A
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique and Université de Bordeaux II, Villenave d'Ornon, France.
Bove
J M
JM
Renaudin
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018388:Codon, Terminator; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D013149:Spiroplasma / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D018388:Codon, Terminator; D004269:DNA, Bacterial; D011938:Rec A Recombinases