Characterization of a flow-sorted human chromosome 10 cosmid library by FISH.
Fluoresence in situ hybridization (FISH) was used to localize cosmids to regions of human chromosome 10. A total of 301 cosmids were selected randomly from a flow-sorted human chromosome 10 cosmid library constructed from human x hamster cell line 762-8A and arrayed in microtiter storage dishes. Over 70% (211/301) of the cosmids mapped to unique regions of chromosome 10. About 7% (22/301) produced multiple hybridization signals indicative of chimeric clones or sequences repeated at low copy number. Three cosmids (3/301, or 1%) hybridized to the centromeric regions of chromosome 10 and one or more other human chromosomes. About 19% (59/301) consisted mostly or entirely of hamster DNA inserts, and about 2% (6/301) appeared to be nonrecombinants.
Ma
N S
NS
Department of Human and Molecular Genetics, Genome Therapeutics Corporation, Waltham, USA.
Zheng
C
C
Benchekroun
Y
Y
Deaven
L L
LL
Longmire
J L
JL
Moir
D T
DT
Mao
J
J
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data
D015342:DNA Probes