Isolation and characterization of gangliosides from Theileria sergenti.
The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGc alpha 2-3Gal beta 1-4Glc beta 1-Cer], SPG (NeuGc) [NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer], i-active ganglioside (NeuGc) [NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer], and I-active ganglioside(NeuGc) [NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3 (Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-6) Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer], respectively.
Watarai
S
S
Department of Cell Chemistry, Okayama University Medical School, Japan.
Sugimoto
C
C
Hosotani-Kaihara
K
K
Kobayashi
K
K
Onuma
M
M
Lee
J T
JT
Kushi
Y
Y
Handa
S
S
Yasuda
T
T
D000740:Anemia / Q000209:etiology / Q000662:veterinary; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000913:Antibodies, Protozoan / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology; D002855:Chromatography, Thin Layer / Q000379:methods / Q000662:veterinary*; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D016794:Theileria / Q000737:chemistry* / Q000276:immunology; D013801:Theileriasis / Q000150:complications / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D005732:Gangliosides; D019158:N-Acetylneuraminic Acid