title: Myosin heavy chain of immature soleus muscle grafts adapts to hyperthyroidism more than to physical activity.
abstract: The interaction of hyperthyroidism and the elements of physical activity on early regeneration of muscle grafts was investigated. Soleus muscle grafts were studied 15 days after graft operations in eu- and hyperthyroid rats. Hypotheses were tested regarding the adaptation of the myosin heavy chain (MHC) profile of grafts and nongrafted control muscles and whether the effect of hyperthyroidism would predominate over the opposing influence of recruitment and mechanical load on MHC of grafts. Denervation and myectomy of synergist muscles were employed to manipulate the elements of physical activity. Denervation decreased the expression of type I MHC, and hyperthyroidism furthered the shift toward a "fast" isoform profile. For example, in denervated grafts, type IIb was undetected in euthyroid rats and accounted for 31% of MHC in hyperthyroid rats. Compared with control muscles, grafts in the denervated and innervated-normal load groups demonstrated greater plasticity because the adaptive response of MHC to thyroid status was more pronounced. Hyperthyroidism predominated over the opposing influence of the elements of physical activity on the regulation of MHC expression in innervated plus overload grafts. For example, type I MHC was 86% of MHC profile of innervated plus overload grafts in euthyroid rats and was only 49% in hyperthyroid rats. In conclusion, a heightened plasticity for grafts was evidenced in denervated and innervated tissues, and the regulation of MHC by thyroid hormone predominated over that due to the elements of physical activity.
authors.affiliation: Department of Human Biodynamics, University of California, Berkeley 94720-4480, USA.
authors.forename: S T
authors.initials: ST
authors.lastname: Devor
authors.forename: T P
authors.initials: TP
authors.lastname: White
mesh_terms: D000818:Animals; D003714:Denervation; D006980:Hyperthyroidism / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000637:transplantation*; D018995:Myosin Heavy Chains / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*
chemical_list: D018995:Myosin Heavy Chains