title: Experimental modification of rat pituitary prolactin cell function during and after spaceflight.
abstract: This study was done to evaluate the effects of microgravity on prolactin (PRL) cells of the male rat pituitary gland. We used the identical passive closed-vial cell culture system that was described for the culture of growth hormone cells (W. C. Hymen, R. E. Grindeland, T. Salada, P. Nye, E. Grossman, and P. Lane. J. Appl. Physiol. 80:955-970, 1996). After an 8-day spaceflight, all flight media (containing released PRL), as well as extracts (containing intracellular PRL), contained significantly lower amounts of immunoreactive PRL than their corresponding ground control samples. On the other hand, these same samples, when assessed for their biological activities by two different in vitro lymphocyte assays, yielded disparate results that may reflect posttranslational modifications to the hormone molecule. Other data showed that 1) the apparent molecular weights of released PRL molecules were not altered by microgravity, but 2) the region from which the PRL cells came (dorsal or ventral) made a significant difference in the amount and activity of PRL released from the flight cells. Because there is much current interest in the role that PRL may play in the regulation of the immune system and because changes in both cellular and humoral immunity accompany spaceflight, this study could help define future microgravity research in this area.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pennsylvania State University 16802, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Hymer
authors.forename: T
authors.initials: T
authors.lastname: Salada
authors.forename: L
authors.initials: L
authors.lastname: Avery
authors.forename: R E
authors.initials: RE
authors.lastname: Grindeland
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010902:Pituitary Gland / Q000502:physiology*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D013997:Time Factors
chemical_list: D011388:Prolactin