title: Early detection of anastomotic leaks after colorectal surgery by measuring endotoxin in the drainage fluid.
abstract: OBJECTIVE
  Early detection of anastomotic leaks after colorectal anastomosis is essential for adequate intervention to prevent peritonitis. We investigated whether the measurement of endotoxin (LPS) concentrations in the drainage has any value for the early detection of anastomotic leaks.
  
  
  METHODS
  Twenty two patients with colorectal anastomosis were enrolled in this study, 3 developed clinically established signs of anastomotic leaks and 19 recovered without complications. LPS concentrations in the drainage, the total daily excreted LPS amounts, leukocyte and thrombocyte counts, plasma urea and creatinine, and body temperature were measured for up to 8 days after surgery and tested for their value to detect anastomotic leaks.
  
  
  RESULTS
  LPS concentrations in the drainage fluid and daily excreted LPS amounts of patients with anastomotic leaks were significantly higher compared to the group without anastomotic leaks. On the third postoperative day, LPS concentrations ranged from 5270 to 6750 pg/ml in patients with anastomotic leaks and from 1 to 1848 pg/ml in patients without complications. Total daily excreted LPS amounts were 270-675 ng/day in patients with anastomotic leak and 0-92 ng/day in patients without anastomotic leaks. Both LPS-related parameters allowed reliable detection of anastomotic leaks on day 3 after surgery (Student's t-Test, p < 0.0005), while leukocyte and thrombocyte counts, plasma urea and creatinine, and body temperatures of both patient groups were not significantly different at any time (p > 0.05).
  
  
  CONCLUSIONS
  We found that the measurement of LPS concentrations in the drainage and the daily excreted LPS amount could be valuable parameters for the early detection of anastomotic leaks as early as on the third post-operative day.
authors.affiliation: II. Chirurgische Abteilung, Landeskrankenanstalt Salzburg, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Junger
authors.forename: W G
authors.initials: WG
authors.lastname: Junger
authors.forename: K
authors.initials: K
authors.lastname: Miller
authors.forename: S
authors.initials: S
authors.lastname: Bahrami
authors.forename: H
authors.initials: H
authors.lastname: Redl
authors.forename: G
authors.initials: G
authors.lastname: Schlag
authors.forename: E
authors.initials: E
authors.lastname: Moritz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000714:Anastomosis, Surgical; D001202:Ascitic Fluid / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000601:surgery*; D004239:Diverticulitis, Colonic / Q000601:surgery*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000175:diagnosis*; D012004:Rectal Neoplasms / Q000601:surgery; D012810:Sigmoid Diseases / Q000601:surgery*; D017792:Surgical Stapling; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides