Serological characterisation of anti-endotoxin sera directed against the conjugates of oligosaccharide core of Escherichia coli type R1, R2, R3, J5 and Salmonella Ra with tetanus toxoid.
The covalent conjugates of oligosaccharide core: Escherichia coli type R1, R2, R3, J5 and Salmonella Ra with tetanus toxoid have been prepared using reaction of reductive amination. The neoglycoconjugates were good immunogens in rabbits yielding a high level of anti-lipopolysaccharide antibodies of IgG class. The antibodies were used to examine the possibility of their reactions with smooth lipopolysaccharides. We have found that all antisera were able to react with the lipopolysaccharide molecules of identical or related core type, possessing core oligosaccharides substituted with O-specific chains. These reactions were shown in both the ELISA assay and the immunoblotting test.
Lugowski
C
C
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland. lugowski@immuno.pan.wroc.pl
Jachymek
W
W
Niedziela
T
T
Rowinski
S
S
D000586:Amination; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D002954:Citrobacter / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D000922:Immunotoxins; D007711:Klebsiella pneumoniae / Q000276:immunology; D019081:O Antigens / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology; D016981:Plesiomonas / Q000276:immunology; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012763:Shigella flexneri / Q000276:immunology; D012764:Shigella sonnei / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D004731:Endotoxins; D007074:Immunoglobulin G; D000922:Immunotoxins; D019081:O Antigens; D009844:Oligosaccharides; D013745:Tetanus Toxoid