Different kinds of antigen-presenting cells exert different effects on T-helper cells development.
It has been well documented that environmental factors such as antigen-presenting cells and related cytokines can affect the development of T-helper cells. The purpose of this study was to investigate the role of different antigen-presenting cells on T-cell development. Ovalbumin (OVA) combined with complete Freud's adjuvant (CFA) was used to sensitize mice subcutaneously or intraperitoneally, and then to follow-up production of IgG and IgE anti-OVA antibodies. In addition, semiquantitative PCR was used to determine the level of cytokine mRNA of different antigen-presenting cells. Resulting data showed that antigen-presenting cells expressed with different characteristics: (1) IgG2a anti-OVA antibody was higher in mice sensitized subcutaneously compared to those sensitized intraperitoneally. (2) The levels of cytokine mRNA were higher in antigen-stimulated spleen cells of mice immunized subcutaneously compared to those of mice immunized intraperitoneally. (3) Langerhans cells expressed a high level of IL-12; in contrast, peritoneal B cells expressed a high level of IL-10, but not IL-12. In summary, cytokine levels such as IL-10 and IL-12 were different among different kinds of APC, and their role in production of different isotypes of antibodies needs further elucidation.
Chuang
Y H
YH
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, ROC.
Chiang
B L
BL
Chou
C C
CC
Hsieh
K H
KH
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers; D005260:Female; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007801:Langerhans Cells / Q000502:physiology*; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D016207:Cytokines; D017931:DNA Primers; D007074:Immunoglobulin G; D012333:RNA, Messenger; D007073:Immunoglobulin E; D010047:Ovalbumin