Enhancement of phagocytosis by corticostatin I (CSI) in cultured mouse peritoneal macrophages.
Corticostatin I (CSI) is one of the corticostatic peptides which inhibit ACTH-stimulated steroidogenesis. To clarify the function of CSI on the immune system, the effect of CSI on phagocytosis by peritoneal macrophages was examined by means of flow cytofluorometry. In the presence of Ca2+ and Mg2+, CSI enhanced phagocytosis of latex beads in a dose-dependent manner. Unstimulated phagocytosis in physiological solution consisted of Ca2+ and Mg(2+)-dependent and -independent phagocytosis. Divalent cations-independent phagocytosis was sensitive to CSI. Present results suggest that the enhancement of phagocytosis by CSI may be one of the mechanisms modulating the immune response regarding infection and inflammation. Present study also showed that one of defensin HNP-1 enhanced phagocytosis.
Ichinose
M
M
Department of Physiology, Shimane Medical University, Izumo, Japan. michinos@shimane-med.ac.jp
Asai
M
M
Imai
K
K
Sawada
M
M
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001798:Blood Proteins / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D023082:Defensins; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006727:Hormone Antagonists / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects*; D023084:alpha-Defensins
D000890:Anti-Infective Agents; D001798:Blood Proteins; D023082:Defensins; D006727:Hormone Antagonists; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C054598:corticostatin; D008274:Magnesium; D002118:Calcium