[Mitochondrial dysfunction in acute lung injury caused by endothelial-1 and leukotoxin].
We studied whether endothelin-1 (ET-1) and leukotoxin (Lx), which have a different effects on vascular tone in isolated perfused rat lungs, also have different effects on mitochondrial function in edematous lung injury. Lung mitochondria were extracted from isolated perfused rat lungs exposed to each mediator. In lungs exposed to 0.5 nmol of ET-1, lung wet weight increased with a markedly elevated perfusion pressure but with no increase in the release of lactate dehydrogenase (LDH), an index of cell damage, into the perfusate. Neither mitochondrial respiration rate no ATP content in the lung tissue differed from those of untreated lungs. In contrast, in lungs treated with 30 mumol of Lx, lung wet weight markedly increased despite a small elevation of perfusion pressure; release of LDH into the perfusate increased, and the mitochondrial respiration rate in state 3 adn 4 significantly decreased while the ATP content in the lung tissue was less than in untreated lungs. We also examined cellular and mitochondrial damage in hydrostatic lung edema caused by raising an outflow reservoir. Mitochondrial respiration was not suppressed, and perfusate LDH activity was not increased, although lung wet weight increased as much as it did after the treatment described above. These results indicate that lung mitochondrial function is differentially affected by ET-1 and Lx, and they suggest that abnormalities in energy production by lung mitochondria are related to permeability edema.
Sakai
T
T
Fukui General Hospital, Japan.
Ishizaki
T
T
Nakai
T
T
Matsukawa
S
S
Hayakawa
M
M
Ozawa
T
T
D000208:Acute Disease; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019332:Endothelin-1 / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005098:Exotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D009929:Organ Size / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011654:Pulmonary Edema / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019332:Endothelin-1; D005098:Exotoxins; D018836:Inflammation Mediators; C018579:leukotoxin; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase