[The change of the amount of receptors on the cell surface after differentiation of human granulocyte cell line HL-60].
In this study, HL-60, a human granulocyte cell line was differentiated into granulocyte-like cell by DMSO and then the change of the amount of Fc receptors and chemotaxis-associated antigen (TM 316) was examined using monoclonal antibodies and fluorescence analysis of cell sorter. The results showed that the amount of chemotaxis-associated antigen increased slightly and chemotactic activity was poor even after HL-60 was well-differentiated. Fc gamma R I (CD 64) and Fc gamma R II (CD 32) receptors in differentiated HL-60 were slightly recognized. However, there was no Fc gamma R III B (CD 16 b) receptor in differentiated HL-60 cells. Although DMSO induced HL-60 was morphologically similar to peripheral granulocytes, differentiated HL-60 was different from peripheral granulocytes in terms of chemotaxis and surface receptors.
Ohse
T
T
Second Department of Internal Medicine, Faculty of Medicine, University of Teikyo.
D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002634:Chemotaxis, Leukocyte; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology*; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011961:Receptors, Fc / Q000032:analysis
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D011961:Receptors, Fc; D004121:Dimethyl Sulfoxide