Human chromosome 6- and 11-encoded factors support human immunodeficiency virus type 1 Rev function in A9 cells.
The precise mechanism of Rev-mediated expression of human immunodeficiency virus (HIV-1) late genes is not well characterized. We recently proposed a requirement for HIV-1 Rev responsive element (RRE) RNA binding host nuclear proteins in Rev function. In this report, using a transient transfection assay of Rev function, we further demonstrate the role of host cell factors in HIV-1 Rev function. Murine A9 cells, which are inefficient in forming RRE-host protein ribonucleoprotein complexes, are also inefficient in supporting Rev function. We also show that host cell factor(s) encoded by human chromosomes 6 and 11 can support HIV-1 Rev-mediated expression of unspliced viral mRNAs in murine A9 cells.
Shukla
R R
RR
Department of Biochemistry, The George Washington University Medical Center, Washington, D.C. 20037, USA.
Marques
S M
SM
Kimmel
P L
PL
Kumar
A
A
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002880:Chromosomes, Human, Pair 11; D002896:Chromosomes, Human, Pair 6; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D016370:Gene Products, rex / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016370:Gene Products, rex; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins; D015500:Chloramphenicol O-Acetyltransferase