title: Platelet adhesion to multimeric and dimeric von Willebrand factor and to collagen type III preincubated with von Willebrand factor.
abstract: As part of a systematic study of platelet interaction with adhesive proteins under flow conditions, we studied platelet adhesion to multimeric and dimeric von Willebrand factor (vWF) coated to glass. vWF-dependent adhesion to collagen type III was studied for comparison. Adhesion to glass-coated vWF and vWF-mediated adhesion to collagen type III were in many respects similar. Both showed no decrease at increasing shear rates and a decline to 50% of maximum with a low-molecular-weight multimeric fraction. Adhesion to glass-coated vWF was partially inhibited by heparin and completely inhibited by prostaglandin I(2) and anti-glycoprotein (GP) Ib and anti-GPIIb-IIIa antibodies. vWF-dependent adhesion to collagen was not inhibited by heparin, was partially inhibited by anti-GPIIb-IIIa, and was completely inhibited by prostaglandin I(2) and anti-GPIb. Recombinant dimeric vWF was made by deletion of the propeptide and expression in Chinese hamster ovary cells. Adhesion was 50% of that with plasma vWF, and larger concentrations of dimeric vWF were required. Adhesion to dimeric vWF was optimal at 1500 s(-1), with a gradual decrease at higher shear rates. We conclude that adhesion to collagen type III is strongly but not completely determined by the adhesive properties of vWF.
authors.affiliation: Department of Hematology, University Hospital, Utrecht, Netherlands.
authors.forename: Y P
authors.initials: YP
authors.lastname: Wu
authors.forename: H H
authors.initials: HH
authors.lastname: van Breugel
authors.forename: H
authors.initials: H
authors.lastname: Lankhof
authors.forename: R J
authors.initials: RJ
authors.lastname: Wise
authors.forename: R I
authors.initials: RI
authors.lastname: Handin
authors.forename: P G
authors.initials: PG
authors.lastname: de Groot
authors.forename: J J
authors.initials: JJ
authors.lastname: Sixma
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000648:ultrastructure; D016466:CHO Cells; D002413:Cations, Divalent / Q000494:pharmacology; D003094:Collagen / Q000502:physiology*; D006224:Cricetinae; D011464:Epoprostenol / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D010973:Platelet Adhesiveness / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000502:physiology; D011956:Receptors, Cell Surface / Q000502:physiology; D013314:Stress, Mechanical; D013997:Time Factors; D014841:von Willebrand Factor / Q000737:chemistry* / Q000502:physiology*
chemical_list: D002413:Cations, Divalent; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D014841:von Willebrand Factor; C028254:von Willebrand factor receptor; D006493:Heparin; D003094:Collagen; D011464:Epoprostenol