Ginsenoside-Rh1 and Rh2 inhibit the induction of nitric oxide synthesis in murine peritoneal macrophages.
The effects of ginsenoside-Rh1 and Rh2 in the induction of nitric oxide (NO) synthesis by IFN-gamma plus LPS were investigated using murine peritoneal macrophages. The NO production from rIFN gamma plus LPS-treated macrophages was markedly reduced by ginsenoside-Rh1 or Rh2 in a dose dependent manner, but had no inhibitory effects by ginsenoside-Rb1, Rc or Re. In addition, treatment of the cells with ginsenoside-Rh2 6 hr before the stimulation with IFN-gamma plus LPS showed more inhibitory effect than the treatment with ginsenoside-Rh2 6 hr after or simultaneously with the stimulation with IFN-gamma plus LPS in the NO production. Ginsenoside-Rh2 also effectively inhibited IFN-gamma induced NO production when the cells were treated with IFN-gamma 6 hr after the treatment with ginsenoside-Rh2. Our findings suggest that this phenomenon might be caused by inhibition of priming signal such as IFN-gamma for the synergistic induction of NO synthesis.
Park
Y C
YC
Department of Molecular Biology, Pusan National University, Korea.
Lee
C H
CH
Kang
H S
HS
Kim
K W
KW
Chung
H T
HT
Kim
H D
HD
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004790:Enzyme Induction; D036145:Ginsenosides; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D005894:Panax; D010529:Peritoneal Cavity / Q000166:cytology; D010946:Plants, Medicinal; D012503:Saponins / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008070:Lipopolysaccharides; D012503:Saponins; D009569:Nitric Oxide; C055305:ginsenoside Rh2; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase