High-performance liquid chromatographic determination of human serum albumin in plasma and urine by post-column fluorescence enhancement detection using 8-anilino-1-naphthalenesulfonic acid.
A method for the quantitative determination of human serum albumin in plasma and urine by post-column high-performance liquid chromatography with fluorescence detection is presented. Human serum albumin in plasma and urine is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection uses the fluorescence enhancement observed when 8-anilino-1-naphthalenesulfonic acid binds to human serum albumin. The method is rapid to perform and is highly sensitive (detection limit is 2.5 ng/injection volume at a signal-to-noise ratio of three.
Ishida
J
J
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Abe
K
K
Nakamura
M
M
Yamaguchi
M
M
D000328:Adult; D000368:Aged; D000419:Albuminuria; D000817:Anilino Naphthalenesulfonates; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D012709:Serum Albumin / Q000032:analysis*
D000817:Anilino Naphthalenesulfonates; D012709:Serum Albumin; C027132:1-anilino-8-naphthalenesulfonate