title: Natural anthracenone subcellular distribution and effects on NADPH-cytochrome P450 reductase microsomal activity.
abstract: Natural anthracenone subcellular distribution and effects on NADPH-cytochrome P450 reductase microsomal activity. Subcellular distribution study of a natural anthracenone (T-514) isolated from Karwinskia humboldtiana showed to be homogeneous on subcellular (nuclear, mitochondrial, peroxisomal and microsomal) fractions prepared from rat liver treated with an acute dose of T-514. These results indicate that T-514 can pass easily through subcellular compartment membranes and an absence of selectivity for some subcellular organelles. A significant increase of protein on liver homogenates and NADPH-cytochrome P450 reductase microsomal activity indicates that T-514 may act as a microsomal enzymatic inducer. In addition, this enzymatic specific activity increment could be due to the interaction of T-514 with the microsomal redox cycling.
authors.affiliation: Departamento de Farmacología y Toxicología, Facultad de Medicina, U.A.N.L., México.
authors.forename: M
authors.initials: M
authors.lastname: Guerrero-Olazarán
authors.forename: J M
authors.initials: JM
authors.lastname: Viader-Salvadó
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D000972:Antineoplastic Agents, Phytogenic / Q000493:pharmacokinetics* / Q000633:toxicity; D004790:Enzyme Induction; D005260:Female; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000187:drug effects* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000873:Anthracenes; D000972:Antineoplastic Agents, Phytogenic; C075149:Karwinskia toxin T-514; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase