Growth inhibition of metronidazole-susceptible and metronidazole-resistant strains of Gardnerella vaginalis by Lactobacilli in vitro.
Metronidazole resistance was produced in susceptible Gardnerella vaginalis after subculture in the presence of metronidazole. Metronidazole-resistant gardnerellae were less susceptible to growth inhibition by Lactobacillus culture filtrates. A low pH (+/- 4) and lactic acid accounted for 60 to 95% of inhibitory activity, and H2O2 accounted for only 0 to 30%. However, in the presence of myeloperoxidase, H2O2-producing lactobacilli decreased the viability of metronidazole-susceptible gardnerellae 2,000-fold.
McLean
N W
NW
Department of Biological Sciences, Glasgow Caledonian University, United Kingdom.
McGroarty
J A
JA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000898:Antibiosis; D004352:Drug Resistance, Microbial; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D008795:Metronidazole / Q000494:pharmacology*; D016585:Vaginosis, Bacterial / Q000382:microbiology
D000900:Anti-Bacterial Agents; D008795:Metronidazole