Expression in a wine yeast strain of the Aspergillus niger abfB gene.
A recombinant wine yeast strain has been constructed expressing the gene coding for alpha-L-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous alpha-L-arabinofuranosidase B shows similar physico-chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The alpha-L-arabinofuranosidase B protein is detected throughout the fermentation.
Sánchez-Torres
P
P
Departamento de Biotecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
González-Candelas
L
L
Ramón
D
D
D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D005285:Fermentation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D014920:Wine / Q000382:microbiology
D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase