DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes.
We found that dimethyl-sulfoxide (DMSO) at concentrations of 2.5% induced apoptosis in SV40-immortalized human keratinocytes, while normal keratinocytes were arrested at the boundary of G1/S phase under the same conditions. DMSO-induced apoptosis in SV-40 immortalized keratinocytes was not associated with change in phosphorylated state of the retinoblastoma susceptibility gene. When SV40-immortalized cells were treated with 2.5% DMSO, dissociation of the complex was observed by immunoblotting of SV40 T antigen from immunoprecipitated p53 protein fraction.
Koike
M
M
Department of Cancer Cell Research, University of Tokyo, Japan.
Ishino
K
K
Kohno
Y
Y
Tachikawa
T
T
Kartasova
T
T
Kuroki
T
T
Huh
N
N
D000957:Antigens, Viral, Tumor / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D016160:Retinoblastoma Protein / Q000378:metabolism; D013539:Simian virus 40 / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; D004121:Dimethyl Sulfoxide