Morphological aspects of endothelial cells cultured in the absence of fetal calf serum under controlled electrical charges applied to the support.
The effect of constant and modulated electrical charges applied through the culture support on endothelial cell (EC) morphology was investigated. Endothelial cells tolerated electrical charges for 48 hours, in a serum-free medium. The EC had normal morphology, except at high stationary charges, for which nucleus and cell size increased. The observed cell divisions and cellular secretions indicated that the cells rapidly adapted to the imposed stresses.
Bouaziz
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CNRS URA 343-Laboratoire de Biorhéologie et d'Hydrodynamique Physico-chimique, Université Paris VII, France.
Richert
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Caprani
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D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002455:Cell Division; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004261:DNA Replication; D004558:Electric Stimulation; D004730:Endothelium, Vascular / Q000166:cytology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D017971:Tin Compounds; D014471:Umbilical Veins / Q000166:cytology
D016895:Culture Media, Serum-Free; D017971:Tin Compounds; C045358:stannic oxide