Expression of tissue-type plasminogen activator, plasminogen activator inhibitor and von Willebrand factor in the supernatant of endothelial cell cultures in response to the seeding of adenocarcinoma cell line HRT-18.
Plasminogen activator inhibitor (PAI-1), tissue type plasminogen activator (tPA) and von Willebrand factor (vWF) concentrations were measured by ELISA in the supernatant of the following cultures: endothelial cells from human umbilical vein (HUVEC); human colon cancer cells (HRT-18); and co-culture cells of HUVEC + HRT-18. No measurable amount of the three substances was found in the supernatant of HRT-18 cell culture. Compared to the value in the HUVEC supernatant, in the UVEC/HRT-18 co-cultures, tPA concentration was significantly lower (P = 0.0047), PAI-1 significantly higher (P = 0.026) and vWF also significantly higher (P = 0.0048). These data indicate that HRT-18 tumor cells do not produce tPA, PAI-1 and vWF; however, these tumor cells induce endothelial cells to change the production of these substances. As a consequence, the interaction between tumor and endothelial cells in vivo may lead to depression of fibrinolysis and enhancement of platelet adhesion.
Morganti
M
M
RWTH Institute of Pathology, Aachen, Germany.
Mittermayer
C
C
Henze
U
U
Carpi
A
A
Sagripanti
A
A
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D018920:Coculture Techniques; D004727:Endothelium / Q000737:chemistry; D004730:Endothelium, Vascular / Q000737:chemistry; D006801:Humans; D015849:Plasminogen Inactivators / Q000032:analysis*; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014471:Umbilical Veins / Q000737:chemistry*; D014841:von Willebrand Factor / Q000032:analysis*
D015849:Plasminogen Inactivators; D014841:von Willebrand Factor; D010959:Tissue Plasminogen Activator