Novel neuropeptide Y processing in human cerebrospinal fluid from depressed patients.
In vitro processing of neuropeptide Y (NPY) in cerebrospinal fluid (CSF) of patients with depression was monitored by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Single peptide bonds in NPY were cleaved to yield N- or C-terminal fragments. Multiple cleavage to form internal peptides was unimportant. Degradation rates varied between individuals, whereas the product distributions were fairly constant. Other peptides did not evidence such proteolysis. MALDI-TOF MS will facilitate extensive investigations of NPY processing that could provide the basis for clinical assays and illuminate the pathophysiology related to depression.
Ekman
R
R
Department of Psychiatry and Neurochemistry, University of Göteborg, Mölndal, Sweden.
Juhasz
P
P
Heilig
M
M
Agren
H
H
Costello
C E
CE
D000328:Adult; D000595:Amino Acid Sequence; D003863:Depression / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000134:cerebrospinal fluid*; D010446:Peptide Fragments / Q000032:analysis / Q000134:cerebrospinal fluid
D009478:Neuropeptide Y; D010446:Peptide Fragments