title: An oxaloacetate decarboxylase homologue protein influences the intracellular survival of Legionella pneumophila.
abstract: Legionella pneumophila is a facultative intracellular parasite which is able to survive in various eukaryotic cells. We characterised a Tn5-mutant of the L. pneumophila Corby strain and were able to identify the insertion site of the transposon. It is localised within an open reading frame which shows high homology to the alpha-subunit of the oxaloacetate decarboxylase (OadA) of Klebsiella pneumoniae. The OadA homologous protein of L. pneumophila was detected in the wild-type strain by Western blotting. Since the intracellular multiplication of the oadA- mutant strain is reduced in guinea pig alveolar macrophages and human monocytes, it is concluded that the oadA gene product has an effect on the intracellular survival of L. pneumophila.
authors.affiliation: Institut für Molekulare Infektionsbiologie, Würzburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Jain
authors.forename: B C
authors.initials: BC
authors.lastname: Brand
authors.forename: P C
authors.initials: PC
authors.lastname: Lück
authors.forename: M
authors.initials: M
authors.lastname: Di Berardino
authors.forename: P
authors.initials: P
authors.lastname: Dimroth
authors.forename: J
authors.initials: J
authors.lastname: Hacker
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D015153:Blotting, Western; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured / Q000166:cytology / Q000382:microbiology; D002876:Chromosomes, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000502:physiology; D006168:Guinea Pigs; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000201:enzymology* / Q000254:growth & development*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000382:microbiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000382:microbiology; D009154:Mutation / Q000502:physiology; D017386:Sequence Homology, Amino Acid
chemical_list: D002262:Carboxy-Lyases; C021916:oxaloacetate decarboxylase