Cloning of the cDNA for a rat intestinal Na+/dicarboxylate cotransporter reveals partial sequence homology with a rat intestinal mucin.
In this report we present the full-length cDNA and deduced 587 amino acid sequence of a putative rat intestinal Na+/dicarboxylate cotransporter. It shows sequence and structural similarity to a rabbit renal Na+/dicarboxylate cotransporter. An unexpected fining was the presence of a C-terminal transmembrane region that is homologous with an 87 amino acid hydrophobic region of a rat intestinal mucin, M2. Mucin-related sequences in transporter proteins have not been described before.
Khatri
I A
IA
Division of Research Biochemistry, Hospital for Sick Children, Toronto, ON, Canada.
Kovacs
S V
SV
Forstner
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D029382:Dicarboxylic Acid Transporters; D007422:Intestines / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D027981:Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D029382:Dicarboxylic Acid Transporters; D008565:Membrane Proteins; D009077:Mucins; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; C092524:SLC13A2 protein, human; C490478:Slc13a2 protein, rat; D027981:Symporters