Cloning, expression pattern and mapping to Xq of NAP1L3, a gene encoding a peptide homologous to human and yeast nucleosome assembly proteins.
From a human fetal brain cDNA library, we isolated a novel gene sharing significant homology with the genes of nucleosome assembly proteins (NAPs). This cDNA clone, designated NAP1L3 (nucleosome assembly protein 1-like 3), contained an open reading frame of 1518 nucleotides encoding 506 amino acids. Its predicted amino acid sequence showed 46% identity and 65% similarity with NAP1L. In its C-terminal half NAP1L3 contained several characteristic motifs strictly conserved with NAP1L and yeast NAP1, but the N-terminal half showed little conservation. Northern-blot analysis revealed strong expression of a 3.0-kb transcript in human adult brain and weak expression in heart. NAP1L3 was closely linked to a marker (DXS990) mapped to chromosome bands Xq21.3-->q22, where genes responsible for several X-linked mental retardation syndromes have been localized.
Watanabe
T K
TK
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd. Tokushima, Japan.
Fujiwara
T
T
Nakamura
Y
Y
Hirai
Y
Y
Maekawa
H
H
Takahashi
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D009928:Organ Specificity; D021382:Protein Sorting Signals; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014960:X Chromosome; D015003:Yeasts / Q000235:genetics
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C533557:NAP1L1 protein, human; C112325:NAP1L3 protein, human; C533558:Nap1l1 protein, mouse; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D021382:Protein Sorting Signals; D011506:Proteins