title: Ordering of human bone marrow B lymphocyte precursors by single-cell polymerase chain reaction analyses of the rearrangement status of the immunoglobulin H and L chain gene loci.
abstract: CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Ghia
authors.forename: E
authors.initials: E
authors.lastname: ten Boekel
authors.forename: E
authors.initials: E
authors.lastname: Sanz
authors.forename: A
authors.initials: A
authors.lastname: de la Hera
authors.forename: A
authors.initials: A
authors.lastname: Rolink
authors.forename: F
authors.initials: F
authors.lastname: Melchers
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000096:biosynthesis; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis; D017931:DNA Primers; D005434:Flow Cytometry; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D018398:Homeodomain Proteins; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D051379:Mice; D018448:Models, Immunological; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D017931:DNA Primers; D018398:Homeodomain Proteins; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; C064658:RAG-1 protein; D004253:DNA Nucleotidylexotransferase