[Expression of recombinant DNA RSVlacZ and pCMVlacZ in preimplantation rabbit embryos].
A linear fragment of the pRK3lacZ plasmid (RSVlacZ) and circular DNA of pCMVlacZ were injected into rabbit zygotes. The embryos were cultured for 24-72 h and analyzed with chromogenic X-gal substrate for bacterial beta-galactosidase. The expression of RSVlacZ DNA was revealed in 6-16-cell embryos. The expression of pCMVlacZ plasmid was low in 2-blastomere embryos, and high in 4-16-cell embryos. In both cases, no beta-galactosidase activity was observed in one-cell embryos. The results obtained indirectly suggest selective and stepwise gene activation in the course of rabbit embryo development.
Gogolevskiĭ
P A
PA
Gogolevskaia
I K
IK
Kuznetsova
I V
IV
Kuznetsov
A V
AV
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001755:Blastocyst; D002874:Chromosome Mapping; D003587:Cytomegalovirus / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics*; D015870:Gene Expression; D008845:Microinjections; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D004270:DNA, Circular
Ekspressiia rekombinantnykh DNK RSVlacZ i pCMVlacZ v predimplantatsionnykh émbrionakh krolika.