title: High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system.
abstract: We succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.
authors.affiliation: Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: R
authors.initials: R
authors.lastname: Taguchi
authors.forename: S
authors.initials: S
authors.lastname: Udaka
authors.forename: H
authors.initials: H
authors.lastname: Ikezawa
mesh_terms: D001407:Bacillus; D001413:Bacillus thuringiensis / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase