title: The ATPase inhibitor protein from bovine heart mitochondria: the minimal inhibitory sequence.
abstract: The mitochondrial ATPase inhibitor subunit is a basic protein of 84 amino acids that helps to regulate the activity of F1F0-ATPase. In order to obtain structural information on the mechanism of inhibition, the bovine inhibitor subunit has been expressed in Escherichia coli and purified in high yield. The recombinant protein has a similar inhibitory activity to the inhibitor subunit isolated from bovine mitochondria. Progressive N-terminal and C-terminal deletion mutants of the inhibitor subunit have been produced either by overexpression and purification, or by chemical synthesis. By assaying the truncated proteins for inhibitory activity, the minimal inhibitory sequence of the inhibitor subunit has been defined as consisting of residues 14-47. The immediately adjacent sequences 10-13 and 48-56 help to stabilize the complex between F1F0-ATPase and the inhibitor protein, and residues 1-9 and 57-84 appear to be dispensable. At physiological pH values, the inhibitor subunit is mainly alpha-helical and forms monodisperse aggregates in solution. Smaller inhibitory fragments of the inhibitor protein, such as residues 10-50, seem to have a mainly random coil structure in solution, but they can adopt the correct inhibitory conformation when they from a complex with the ATPase. However, these latter fragments are mainly monomeric in solution, suggesting that the aggregation of the inhibitor subunit in solution may be due to intermolecular alpha-helical coiled-coil formation via the C-terminal region. The noninhibitory peptides consisting of residues 10-40 and 23-84 of the inhibitor protein can bind to F1F0-ATPase, and interfere with inhibition by the intact inhibitor subunit. The noninhibitory fragments of the inhibitor protein consisting of residues 22-46 and 44-84 do not compete with the inhibitor subunit for its binding site on F1F0-ATPase.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, U.K.
authors.forename: M J
authors.initials: MJ
authors.lastname: van Raaij
authors.forename: G L
authors.initials: GL
authors.lastname: Orriss
authors.forename: M G
authors.initials: MG
authors.lastname: Montgomery
authors.forename: M J
authors.initials: MJ
authors.lastname: Runswick
authors.forename: I M
authors.initials: IM
authors.lastname: Fearnley
authors.forename: J M
authors.initials: JM
authors.lastname: Skehel
authors.forename: J E
authors.initials: JE
authors.lastname: Walker
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003854:Deoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000235:genetics; D012542:Scattering, Radiation; D016415:Sequence Alignment; D000097795:ATPase Inhibitory Protein
chemical_list: D000255:Adenosine Triphosphate; D003854:Deoxyribonucleotides; D004791:Enzyme Inhibitors; D010446:Peptide Fragments; D011506:Proteins; D006180:Proton-Translocating ATPases; D011994:Recombinant Proteins; D000097795:ATPase Inhibitory Protein