The human homolog T of the mouse T(Brachyury) gene; gene structure, cDNA sequence, and assignment to chromosome 6q27.
We have cloned the human gene encoding the transcription factor T. T protein is vital for the formation of posterior mesoderm and axial development in all vertebrates. Brachyury mutant mice, which lack T protein, die in utero with abnormal notochord, posterior somites, and allantois. We have identified human T genomic clones and derived the mRNA sequence and gene structure. There is 91% amino acid identity between human and mouse T proteins overall and complete identity across 77 amino acids of the T-box motif within the DNA-binding domain. Human T expression is very similar to that found for T in other vertebrate species and is confined to cells derived from the notochord. The human T gene maps to chromosome 6q27 and is only the second human member of the T-box gene family to be described.
Edwards
Y H
YH
Human Biochemical Genetics Unit, University College London, England. yedwards@hgmp.mrc.ac.uk
Putt
W
W
Lekoape
K M
KM
Stott
D
D
Fox
M
M
Hopkinson
D A
DA
Sowden
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005326:Fetal Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000235:genetics*; D000098908:Brachyury Protein
D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D000098908:Brachyury Protein