[Evaluation of a new technique of extracorporeal perfusion of the swine liver on a swine-primate model].
With the increasing success of liver transplantation there is an urgent need for developing an artificial liver support system to be used in patients with liver failure. An extracorporeal porcine liver perfusion machine was successfully tested in animals with experimental liver failure. Livers were flushed, removed from 35 kg pigs and placed in a heated sterile cassette. The portal vein and the hepatic artery of the graft were connected to the arterial system of the animals. The perfusion pressure of the hepatic artery was regulated via a pressure-flow computerized feed-back device. The venous flow was reinfused from the hepatic veins of the graft to the jugular vein of the animals. The experimental work consisted in two steps: 1. evaluation of clinical and biological consequences of liver perfusion in healthy animals (Group A = pigs, n = 3; group B = primates, n = 3); 2. evaluation of the efficiency of the liver perfusion in animals with ischemic liver failure (Groupe D = pigs, n = 6). The control group (Group C = pigs, n = 7) consisted of pigs with ischemic liver failure without hepatic support. No major clinical or biological adverse effects are reported in groups A and B excepted a thrombocytmia and a marked increase in serum transaminases levels in group B. Liver function as assessed by the bile flow was good in both groups. Comatose pigs with ischemic hepatic failure (group D) recovered a subnormal neurological status in five out of six cases. Serum ammoniemia level were significantly decreased (from 1076 +/- 163 to 255 +/- 32 umol/l). A decrease in serum bilirubine levels and an improvement in the coagulation profile were observed in the perfused animals. Pigs and primates tolerated the perfusion procedure well and beneficial effects were observed in perfused pigs with experimental liver failure.
Beaufigeau
M
M
Fondation Transplantation, Strasbourg.
Wolf
P
P
Azimzadeh
A
A
Godfrin
Y
Y
Beller
J P
JP
Boudjema
K
K
Jaeck
D
D
Kieny
R
R
Cinqualbre
J
J
D000818:Animals; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D006801:Humans; D008102:Liver Circulation; D017114:Liver Failure, Acute / Q000628:therapy*; D008251:Macaca; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D013552:Swine
Evaluation d'une nouvelle technique de perfusion extracorporelle de foie de porc sur un modèle porc-primate.