Generation and mapping of human chromosome 2 microdissection clone-derived STSs.
Twenty-nine new sequence-tagged sites (STSs) were derived from DNA sequences of clones from two human chromosome 2 microdissection libraries. The specificity of the STSs for human chromosome 2 was first demonstrated by PCR amplification of DNA from genomic human and hamster cells and a human chromosome 2-containing human x hamster hybrid cell line. The STSs were then mapped to chromosome 2 by two different approaches. In the first attempt, 12 of the STSs were shown to PCR amplify YAC clones associated with genetic markers on the chromosome. In the second approach, 27 of the STSs were localized to chromosome bands by FISH using cosmid or PAC clones encoding the STSs. The specific STSs mapped to chromosome 2 by these two approaches tie together the genetic and cytogenetic maps of the chromosome at the two termini. The distribution of these STSs further defines the region of the chromosome present in the two microdissection libraries.
Gingrich
J C
JC
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, CA, USA. jgingrich@bio-rad.com
Garnes
J A
JA
Lee
D A
DA
Boehrer
D M
DM
D002871:Chromosome Banding; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D017931:DNA Primers