title: Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei.
abstract: Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.
authors.affiliation: Victorian Infectious Diseases Reference Laboratory, Fairfield Hospital, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Stenos
authors.forename: B
authors.initials: B
authors.lastname: Ross
authors.forename: H M
authors.initials: HM
authors.lastname: Feng
authors.forename: P
authors.initials: P
authors.lastname: Crocquet-Valdes
authors.forename: D
authors.initials: D
authors.lastname: Walker
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D004269:DNA, Bacterial; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial