The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease.
The Saccharomyces cerevisiae APN1 gene, encoding the bifunctional DNA repair enzyme apurinic/apyrimidinic (AP) endonuclease/3'-repair diesterase, was used as a probe to isolate a gene homolog, CeAPN1, from a Caenorhabditis elegans cDNA library. The CeAPN1 gene is predicted to encode a protein 30 kDa in size, which shares 40.4% and 44.9% identity at the amino acid level with, respectively, S. cerevisiae Apn1 and Escherichia coli endonuclease IV. We suggest that CeApn1 protein is a member of the endonuclease IV family of DNA repair enzymes.
Masson
J Y
JY
CHUL Research Center, Health and Environment Unit, Sainte-Foy, Québec,Canada.
Tremblay
S
S
Ramotar
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D045643:DNA Repair Enzymes; D018509:DNA, Helminth; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D029968:Escherichia coli Proteins; D015801:Helminth Proteins; D029701:Saccharomyces cerevisiae Proteins; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; C083274:Apn1 protein, S cerevisiae; D008190:Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; C102951:apn-1 protein, C elegans; D045643:DNA Repair Enzymes