Changes in blood lymphocyte populations in experimental bowel allograft rejection.
The aim of this study is to measure percentages of lymphocyte populations and IL-2R cellular expression in peripheral blood during the rejection of a small bowel allograft (SBA) in the rat. Thirty rats were allotted to three groups: A, control, no transplantation (Tx); B, rats receiving an orthotopic SBA; C, similar SBA but with thymostimulin (TP-1) administered before Tx, aimed at increasing the intensity of and accelerating rejection. The percentages of CD19, CD5, CD4 and CD8 cells and of IL-2R were determined when rejection was present. Rejection appeared in rats in group B between days 11 and 26 post-Tx and in group C between days 6 and 7 post-Tx (P < 0.001). In both B and C groups, CD5 and CD4 cells decreased (P < 0.005) and CD8 cells increased (P < 0.001). A correlation between CD8 and IL-2R content was found (P < 0.05). In group C, earliness of rejection correlated with the percentage of CD8 cells (P < 0.05) and the intensity of rejection with numbers of CD8 and CD19 cells (P < 0.05).
Navarro-Zorraquino
M
M
Department of Surgery, University of Zaragoza, Spain.
Güemes
A
A
Lozano
R
R
Larrad
L
L
Morandeira
M J
MJ
Salinas
J C
JC
Pastor
C
C
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D006084:Graft Rejection; D007421:Intestine, Small / Q000637:transplantation*; D016131:Lymphocyte Subsets / Q000502:physiology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D011920:Rats, Inbred WF; D015375:Receptors, Interleukin-2 / Q000032:analysis
D015375:Receptors, Interleukin-2