Cloning and sequencing of the gene encoding alpha-acetolactate decarboxylase from Leuconostoc oenos.
The alsD gene encoding alpha-acetolactate decarboxylase was isolated from a genomic library of Leuconostoc oenos, using a screening procedure developed on microtiter plates. The nucleotide sequence of alsD encodes a putative protein of 239 amino acids showing significant similarity with other bacterial alpha-acetolactate decarboxylases. Upstream from alsD lies an open reading frame (alsS) which is highly similar to bacterial genes coding for catabolic alpha-acetolactate synthases. Northern (RNA) blotting analyses indicated the presence of a 2.4-kb dicistronic transcript of alsS and alsD. This suggests that the alsS and alsD genes are organized in a single operon.
Garmyn
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D
Laboratoire de Microbiologie, ENSBANA, Université de Bourgogne, Dijon, France. garmyn@u-bourgogne.fr
Monnet
C
C
Martineau
B
B
Guzzo
J
J
Cavin
J F
JF
Diviès
C
C
D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial / Q000235:genetics; D007773:Lactates / Q000378:metabolism; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D012329:RNA, Bacterial / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D007773:Lactates; D012329:RNA, Bacterial; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase