The genomes of most animals have multiple members of the Tc1 family of transposable elements.
A PCR assay was employed to detect sequence homologous to the transposase gene of the Tc1 family of transposable elements in a wide variety of animals. Amplification products of the appropriate size were obtained from most insects (92 of 108 examined; 85%), most other invertebrates (33 of 43; 77%), and many vertebrates (18 of 36; 50%). Sequencing a sample of cloned PCR products from eight insects, one hydra, and two frogs revealed that each had multiple distinct members of the family in their genomes. In the most extreme case, the horn fly Haematobia irritans yielded evidence of seventeen distinct types of Tc1 family elements. Most of the sequences obtained indicate that the elements are within the range of variation already known from fungi, nematodes, flies, fish and frogs. Some, however, had novel length variants or divergent sequences, indicating that they represent new subfamilies of these transposons. These results indicate that this family of transposons is extremely common in animal genomes, with multiple representatives in most genomes.
Avancini
R M
RM
Department of Entomology, University of Illinois, Urbana 61801, USA.
Walden
K K
KK
Robertson
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics; D007313:Insecta; D007448:Invertebrates; D008322:Mammals; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D019895:Transposases; D014714:Vertebrates
D004251:DNA Transposable Elements; D004254:DNA Nucleotidyltransferases; D019895:Transposases