The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli.
A kinetic analysis of secretion of lipo-chitin oligosaccharides (LCO) produced by Rhizobium etli (Re) wild-type (wt) strain and derivatives carrying disrupted nodI or nodJ genes was performed. LCO were detected in the growth media of the wt strain as early as 1 h after nod gene induction. In contrast, strains carrying nodI or nodJ mutations secreted less LCO, and accumulated LCO metabolites intracellularly after 4 h of induction. These Re mutants presented a delayed nodulation phenotype and a reduction in the maximum number of nodules formed in Phaseolus vulgaris roots.
Cárdenas
L
L
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Curenavaca, Morelos CP62271, Mexico.
Domínguez
J
J
Santana
O
O
Quinto
C
C
D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D007887:Fabaceae / Q000382:microbiology; D005798:Genes, Bacterial; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009154:Mutation; D009586:Nitrogen Fixation; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; C023023:lipid-linked oligosaccharides; C065618:nodI protein, Bacteria; C489991:nodJ protein, Rhizobium etli; D002686:Chitin