title: Thiopentone and propofol, but not methohexitone nor midazolam, inhibit neutrophil oxidative responses to the bacterial peptide FMLP.
abstract: The effects of different anaesthetics on the neutrophil oxidative response in vitro are compared. Neutrophils were stimulated with small amounts of the bacterial peptide N-formyl-L-methionyl-L-leucyl-phenylalanine as a physiological, receptor-dependent stimulus. A new flow cytometry-based method capable of detecting the small amounts of H2O2 generated by neutrophils in a heterogeneous all-or-none response following submaximal stimulation with N-formyl-L-methionyl-L-leucyl-phenylalanine was used. Propofol and thiopentone suppressed the respiratory burst significantly (P < 0.05 and P < 0.01) in therapeutic concentrations, while midazolam and methohexitone inhibited significantly (P < 0.01) only at concentrations greater than the therapeutic range. Impairment of the neutrophil response was primarily because of a reduction in the number of neutrophils participating in the respiratory burst and not of a proportional decrease of the fluorescence of all neutrophils.
authors.affiliation: Department of Anaesthesia, University of Regensburg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Fröhlich
authors.forename: G
authors.initials: G
authors.lastname: Rothe
authors.forename: B
authors.initials: B
authors.lastname: Schwall
authors.forename: G
authors.initials: G
authors.lastname: Schmitz
authors.forename: J
authors.initials: J
authors.lastname: Hobbhahn
authors.forename: K
authors.initials: K
authors.lastname: Taeger
mesh_terms: D000328:Adult; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D008723:Methohexital / Q000494:pharmacology; D008874:Midazolam / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D015742:Propofol / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D012978:Sodium Oxybate / Q000494:pharmacology; D013874:Thiopental / Q000494:pharmacology
chemical_list: D018686:Anesthetics, Intravenous; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012978:Sodium Oxybate; D006861:Hydrogen Peroxide; D008723:Methohexital; D013874:Thiopental; D008874:Midazolam; D015742:Propofol