Genotoxic evaluation of the herbicide trifluralin on human lymphocytes exposed in vitro.
The herbicide trifluralin was evaluated for genotoxicity in cultured human peripheral blood lymphocytes. Sister-chromatid exchanges (SCE), chromosome aberrations (CA) and micronuclei (MN) were scored as genetic endpoints. To detect eventual metabolic modification in the genotoxicity of this herbicide, the cultures for SCE and MN demonstration were also treated with S9 fraction. From our results we can conclude that trifluralin was able to exert a weak cytotoxic effect, reducing both the proliferative rate index (PRI) and the cytokinesis block proliferation index (CBPI), and also to induce a slight but statistically significant increase in the frequency of SCE. Under our conditions of testing, no genotoxic effects of trifluralin were observed in the CA and MN assays.
Ribas
G
G
Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Surrallés
J
J
Carbonell
E
E
Xamena
N
N
Creus
A
A
Marcos
R
R
D002478:Cells, Cultured; D006540:Herbicides / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012680:Sensitivity and Specificity; D014274:Trifluralin / Q000633:toxicity*
D006540:Herbicides; D009153:Mutagens; D014274:Trifluralin