Distribution of plasma cell markers and intracellular IgE in cell line U266.
The IgE-producing plasma cell line U266 was used to study intracellular IgE and the distribution of the plasma cell markers CD38 and PCA-1. We found two separate clusters of U266 based on the differences in their light scatter properties in the flow cytometer. One cluster displayed high scatter signals (high SS), whereas the other showed low scatter signals (low SS). Most (75%) of the cells in the low SS cluster were positive for intracellular IgE. In contrast, high SS cells had a higher expression of surface IgE and a considerably lower proportion of these cells expressed intracellular IgE. Different patterns of expression of the plasma cell antigens CD38 and PCA-1 were observed. While the low SS cluster displayed a high (76%) expression of PCA-1 and a low (5%) CD38 expression, the high SS cluster expressed 21 and 42%, respectively. About 2/3 of the cells in the low SS cluster appeared to be in the G1 phase of the cell cycle while 1/3 were in the S/G2 phase. In the high SS cluster the opposite distribution (2/3 in S/G2) was observed. Taken together our results indicate an association between cell cycle stages, expression of intracellular IgE and plasma cell markers.
Lagging
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Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden.
van Hage-Hamsten
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M
Härfast
B
B
Halldén
G
G
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D018941:Antigens, CD19 / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000378:metabolism; D007424:Intracellular Fluid / Q000276:immunology*; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases / Q000032:analysis*; D010950:Plasma Cells / Q000276:immunology*; D010954:Plasmacytoma / Q000276:immunology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D018941:Antigens, CD19; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D007073:Immunoglobulin E; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1