The mammalian transcriptional repressor RBP (CBF1) regulates interleukin-6 gene expression.
The cellular interleukin-6 (IL-6) gene contains a target site for the mammalian transcriptional repressor RBP. The target site is contained within the interleukin response element (ILRE), which mediates IL-6 activation by NF-kappa B. In this study, we show by using transient-expression assays that RBP represses activated transcription from the IL-6 gene. The presence and position of the RBP target site are crucial in mediating repression by RBP. While RBP binds within the ILRE, it does not target NF-kappa B alone; nonetheless, NF-kappa B binding to the ILRE is required for repression. Our results indicate that RBP represses coactivation by NF-kappa B and another cellular transcription factor, C/EBP-beta.
Kannabiran
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Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635, USA.
Zeng
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Vales
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D016475:3T3 Cells; D000818:Animals; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D019556:COS Cells; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000502:physiology*; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D015850:Interleukin-6 / Q000235:genetics*; D007378:Interleukins; D051379:Mice; D009154:Mutation; D016328:NF-kappa B / Q000494:pharmacology; D009687:Nuclear Proteins / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D051996:Transcription Factor RelA; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D015850:Interleukin-6; D007378:Interleukins; D016328:NF-kappa B; D009687:Nuclear Proteins; D012333:RNA, Messenger; C497571:Rbpj protein, mouse; D012097:Repressor Proteins; D051996:Transcription Factor RelA