Estimation of heterozygosity in Ribes nigrum L. using RAPD markers.
Heterozygosity in three cultivars of the blackcurrant (Ribes nigrum L.) was estimated. A selfed population of each cultivar was screened for Random Amplified Polymorphic DNA markers (RAPDs) and heterozygous loci were identified by band segregation in contrast with the non-segregation of homozygous loci. On average, 21% of the loci scored in each cultivar were heterozygous. The implications for mapping studies in Ribes nigrum are discussed.
Lanham
P G
PG
Soft Fruit and Perennial Crops Department, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK.
D001483:Base Sequence; D017931:DNA Primers; D006580:Genetic Carrier Screening; D005819:Genetic Markers; D006720:Homozygote; D010945:Plants, Edible / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D005819:Genetic Markers