title: Isolation and characterization of the chicken m2 acetylcholine receptor promoter region: induction of gene transcription by leukemia inhibitory factor and ciliary neurotrophic factor.
abstract: We have isolated the promoter region and determined the start sites of transcription for the gene encoding the chicken m2 (cm2) muscarinic acetylcholine receptor. Transfection experiments, using cm2-luciferase reporter gene constructs, demonstrated that a 789-bp genomic fragment was sufficient to drive high level expression in chicken heart primary cultures, while an additional 1.2-kb region was required for maximal expression in mouse septal/ neuroblastoma (SN56) cells. Treatment of SN56 cells with the cytokines ciliary neurotrophic factor and leukemia inhibitory factor increases expression of endogenous muscarinic acetylcholine receptors and results in a 4- to 6-fold induction of cm2 promoter driven luciferase expression. We have mapped a region of the cm2 promoter that is necessary for induction by cytokines.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle 98195-7750, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Rosoff
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: N M
authors.initials: NM
authors.lastname: Nathanson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping; D020934:Ciliary Neurotrophic Factor; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D006131:Growth Inhibitors / Q000235:genetics / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000235:genetics / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011976:Receptors, Muscarinic / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D020934:Ciliary Neurotrophic Factor; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D009419:Nerve Tissue Proteins; D011976:Receptors, Muscarinic