title: Chronic low-dose UVA irradiation induces local suppression of contact hypersensitivity, Langerhans cell depletion and suppressor cell activation in C3H/HeJ mice.
abstract: It has previously been demonstrated that chronic low-dose solar-simulated UV radiation could induce both local and systemic immunosuppression as well as tolerance to a topically applied hapten. In this study, we have used a chronic low-dose UV-irradiation protocol to investigate the effects of UVA on the skin immune system of C3H/ HeJ mice. Irradiation with UVA + B significantly suppressed the local and systemic primary contact hypersensitivity (CHS) response to the hapten 2,4,6-trinitrochlorobenzene. Furthermore UVA + B reduced Langerhans cell (LC) and dendritic epidermal T cell (DETC) densities in chronically UV-irradiated mice. Ultraviolet A irradiation induced local, but not systemic, immunosuppression and reduced LC (32%) but not DETC from the epidermis compared to the shaved control animals. Treatment of mice with both UVA + B and UVA radiation also induced an impaired secondary CHS response, and this tolerance was transferable with spleen cells. These results suggest that depletion of LC, but not DETC, may be involved in UVA-induced local immunosuppression in our model, and that tolerance was induced in the presence of normal numbers of DETC. Hence exposure of C3H/HeJ mice 5 days per week for 4 weeks with UVA can induce local immunosuppression and tolerance.
authors.affiliation: Department of Medicine (Dermatology), University of Sydney, Royal Prince Alfred Hospital, New South Wales, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Bestak
authors.forename: G M
authors.initials: GM
authors.lastname: Halliday
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000517:prevention & control*; D005260:Female; D007801:Langerhans Cells / Q000166:cytology / Q000528:radiation effects*; D008213:Lymphocyte Activation / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000528:radiation effects*; D014466:Ultraviolet Rays