Plasma levels of chromogranin A are directly proportional to tumour burden in neuroblastoma.
A novel animal experimental model involving the human, poorly differentiated, and adrenergic neuroblastoma cell line SH-SY5Y xenotransplanted to subcutaneous tissue of 13 nude rats (WAG rnu/rnu) was used to investigate the usefulness of six proposed neuroblastoma markers. It was shown that the plasma concentrations of human chromogranin A (CgA) as measured by RIA were directly proportional to tumour volume (r = 0.83, P < 0.001). To rule out possible liberation of CgA by tumour cell lysis, the CgA degradation product pancreastatin was also measured in plasma by a specific RIA, but was not detectable. Plasma neurone-specific enolase (NSE) was elevated in tumour-bearing animals (P < 0.01), but did not correlate with tumour volume (r = 0.49, P > 0.05). Urine homovanillic acid (HVA), detected by HPLC, was elevated in tumour-bearing animals (P < 0.01), but did not correlate with tumour volume (r = -0.32, P > 0.05). Urine vanillyl mandelic acid was not detectable. Urine dopamine was found in low concentrations that did not correlate with tumour volume. In summary, although plasma NSE and urinary HVA were elevated in tumour-bearing animals only plasma CgA correlated with tumour burden. This makes CgA a promising biochemical marker for neuroblastomas.
Wassberg
E
E
Department of Human Anatomy, University of Uppsala, Sweden.
Stridsberg
M
M
Christofferson
R
R
D000310:Adrenal Gland Neoplasms / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D053379:Chromogranin A; D002864:Chromogranins / Q000097:blood*; D005260:Female; D006719:Homovanillic Acid / Q000652:urine; D008297:Male; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D010187:Pancreatic Hormones / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood; D051381:Rats; D011923:Rats, Nude; D014407:Tumor Cells, Cultured / Q000637:transplantation
D014408:Biomarkers, Tumor; C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D010187:Pancreatic Hormones; C506153:chromogranin A, rat; C050836:pancreastatin; D010751:Phosphopyruvate Hydratase; D006719:Homovanillic Acid