title: [Disinfection of human donor corneas during preparation and preservation].
abstract: Endophthalmitis after penetrating keratoplasty is rare but more frequently observed than after other intraocular procedures. The main source may be a contaminated donor cornea. This survey reviews current concepts of donor globe decontamination and avoidance of infection during preservation. Basically, we suggest the use of a 3% PVP-Iodine solution for decontamination of donor globes. Using short-term preservation techniques, the tissue should be stored at ambient temperature after preparation of corneoscleral disc or before transplantation for a while to allow antibiotics to act against decontaminants. Tissue stored by organ culture should be kept in a sealed vial. Alternatively, the medium may be changed every 10 to 14 days under sterile conditions.
authors.affiliation: Augenklinik, Medizinische Hochschule Hannover.
authors.forename: M
authors.initials: M
authors.lastname: Hagenah
authors.forename: R
authors.initials: R
authors.lastname: Winter
mesh_terms: D004203:Disinfection / Q000379:methods*; D009877:Endophthalmitis / Q000517:prevention & control*; D006801:Humans; D015948:Keratoplasty, Penetrating; D009926:Organ Preservation / Q000379:methods*; D012307:Risk Factors; D013242:Sterilization / Q000379:methods*; D013530:Surgical Wound Infection / Q000517:prevention & control*
vernacular_title: Dekontamination menschlicher Spenderhornhäute während der Präparation und Konservierung.