title: Exon mapping by fiber-FISH or LR-PCR.
abstract: In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of >/=200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.
authors.affiliation: Department of Cytochemistry and Cytometry, State University Leiden, Wassenaarseweg 72, Leiden, 2333 AL, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Florijn
authors.forename: F M
authors.initials: FM
authors.lastname: van de Rijke
authors.forename: H
authors.initials: H
authors.lastname: Vrolijk
authors.forename: L A
authors.initials: LA
authors.lastname: Blonden
authors.forename: M H
authors.initials: MH
authors.lastname: Hofker
authors.forename: J T
authors.initials: JT
authors.lastname: den Dunnen
authors.forename: H J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ommen
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D003360:Cosmids / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000648:ultrastructure; D004274:DNA, Recombinant / Q000235:genetics* / Q000648:ultrastructure; D016189:Dystrophin / Q000235:genetics; D005091:Exons / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D009136:Muscular Dystrophies / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites
chemical_list: D018076:DNA, Complementary; D004274:DNA, Recombinant; D016189:Dystrophin