title: [Role of Na(+)-glucose cotransport in fluid absorption across alveolar epithelium in isolated rat lungs].
abstract: To evaluate the role of Na(+)-glucose cotransport in fluid absorption across alveolar epithelial walls in isolated rat lungs, we measured the inhibitory effects of amiloride (a Na+ channel blocker) and phlorizin (a Na(+)-glucose cotrasport blocker) on the fluid absorption rate in fluid-filled lungs. Amiloride (10(-5)-10(-4) M) reduced alveolar fluid absorption by 30%. This value was similar to that obtained in the presence of 10(-3) M phlorizin. The coefficient of Na(+)-glucose cotransport was estimated to be 2.5. The strong correlation between Na+ escape and fluid absorption (r = 0.907) was not affected by phlorizin. These findings suggest that the impact of Na(+)-glucose cotransport was similar to that of Na+ transport alone, and that glucose molecules transported by Na(+)-glucose cotransport do not play an important role in alveolar fluid absorption across rat alveolar epithelium.
authors.affiliation: Department of Thoracic Surgery, Tohoku University.
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Noda
authors.forename: M
authors.initials: M
authors.lastname: Sugita
authors.forename: T
authors.initials: T
authors.lastname: Tanita
authors.forename: S
authors.initials: S
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Sakuma
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: S
authors.initials: S
authors.lastname: Fujimura
mesh_terms: D000042:Absorption; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000502:physiology*; D010695:Phlorhizin / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000378:metabolism*; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects
chemical_list: D009004:Monosaccharide Transport Proteins; D015222:Sodium Channels; D000584:Amiloride; D010695:Phlorhizin