Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain.
Two genes (ptsI and ptsA) that encode homologues of the energy coupling Enzyme I of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system (PTS) have previously been identified on the Escherichia coli chromosome. We here report the presence of a third E. coli gene, designated ptsP, that encodes an Enzyme I homologue, here designated Enzyme INtr. Enzyme INtr possesses an N-terminal domain homologous to the N-terminal domains of NifA proteins [(127 amino acids (aa)] joined via two tandem flexible linkers to the C-terminal Enzyme I-like domain (578 aa). Structural features of the putative ptsP operon, including transcriptional regulatory signals, are characterized. We suggest that Enzyme INtr functions in transcriptional regulation of nitrogen-related operons together with previously described PTS proteins encoded within the rpoN operon. It may thereby provide a link between carbon and nitrogen assimilatory pathways.
Reizer
J
J
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA. jreizer@ucsd.edu
Reizer
A
A
Merrick
M J
MJ
Plunkett
G
G
Rose
D J
DJ
Saier
M H
MH
D000818:Animals; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D006224:Cricetinae; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D051877:RNA Polymerase Sigma 54; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012486:Salmonella typhimurium / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C061338:NifA protein, Bacteria; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C497751:rpoN protein, E coli; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54