Human gingival keratinocytes express E-selectin (CD62E).
OBJECTIVE
E-selectin (CD62E) is an adhesion molecule that participates in the binding of leukocytes to activated blood vascular endothelium. The present study was undertaken to characterise the pattern of E-selectin expression on epithelial cells of the gingival crevice and oral epithelium.


METHODS
A panel of six anti-E-selectin monoclonal antibodies was reacted with cryosections of human gingiva and cytospins of cultured gingival keratinocytes.


RESULTS
Three antibodies raised against leukocyte binding epitopes of the molecule (H4/18, H18/7, 1.2B6) were reactive with gingival keratinocytes, three were negative (4D10, BBA1, BBA2), while all six reacted with endothelial cells. Staining with H18/7 and 1.2B6 was weaker and more variable than with H4/18. In cell culture, levels of E-selectin expression reduced slowly, and were only modestly increased by treatment with exogenous TNF alpha, a known inducer of E-selectin on endothelium.


CONCLUSIONS
E-selectin epitopes are expressed on gingival keratinocytes. These binding epitopes may be involved in the traffic of leukocytes in this tissue compartment.
Pietrzak
E R
ER
Department of Dentistry, University of Queensland, Brisbane, Australia.
Savage
N W
NW
Walsh
L J
LJ
D000328:Adult; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis*; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D000939:Epitopes; D005260:Female; D005881:Gingiva / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D000939:Epitopes; D014409:Tumor Necrosis Factor-alpha