title: Significance of HPr in catabolite repression of alpha-amylase.
abstract: CcpA and HPr are presently the only two proteins implicated in Bacillus subtilis global carbon source catabolite repression, and the ptsH1 mutation in the gene for the HPr protein was reported to relieve catabolite repression of several genes. However, alpha-amylase synthesis by B. subtilis SA003 containing the ptsH1 mutation was repressed by glucose. Our results suggest HPr(Ser-P) may be involved in but is not required for catabolite repression of alpha-amylase, indicating that HPr(Ser-P) is not the sole signaling molecule for CcpA-mediated catabolite repression in B. subtilis.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
authors.forename: M I
authors.initials: MI
authors.lastname: Voskuil
authors.forename: G H
authors.initials: GH
authors.lastname: Chambliss
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D004794:Enzyme Repression; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D000516:alpha-Amylases; D005947:Glucose