Effects of 1, 9-dideoxy forskolin on delayed rectifier potassium current of bullfrog dorsal root ganglion cells.
Whole-cell voltage-clamp recordings were made from cultured bullfrog dorsal root ganglion cells to examine the channel blocking activity of 20 microM of 1, 9-dideoxy forskolin (d-FSK) on the delayed rectifier potassium current. The drug reduced the maximum amplitude of the current (10-20 nA at +65 mV) by 40-80%. However, the voltages for 50%-activation (approximately -12 mV) and for 50%-inactivation (approximately -37 mV) remained unaffected by d-FSK. These results indicate that the channel block by d-FSK occurs in a voltage-independent manner.
Tokimasa
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Department of Physiology, School of Medicine, Tokai University, Kanagawa, Japan.
D000818:Animals; D002478:Cells, Cultured; D005576:Colforsin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051668:Delayed Rectifier Potassium Channels; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D011892:Rana catesbeiana
D051668:Delayed Rectifier Potassium Channels; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D005576:Colforsin; C047983:1,9-dideoxyforskolin