A new role for RNase II in mRNA decay: striking differences between RNase II mutants and similarities with a strain deficient in RNase E.
The effect of Escherichia coli ribonuclease II and polynucleotide phosphorylase was analysed on the degradation of Desulfovibrio vulgaris cytochrome c3 (cyc) mRNA. In the absence of these exoribonucleolytic activities, cyc mRNA was stabilised but the two enzymes had a different role in its decay. Surprisingly, a temperature-sensitive mutation in ribonuclease II gave a degradation pattern similar to what had been observed in the absence of endoribonuclease E activity. In an RNase II deletion mutant this was not observed. We propose and verify a model in which the temperature-sensitive ribonuclease II interferes with the action of ribonuclease E.
Cruz
A A
AA
Instituto de Tecnologia Química e Biológica, Unviersidade Nova de Lisboa, Oeiras, Portugal.
Marujo
P E
PE
Newbury
S F
SF
Arraiano
C M
CM
D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D015152:Blotting, Northern; D003574:Cytochrome c Group / Q000235:genetics / Q000378:metabolism; D016969:Desulfovibrio vulgaris / Q000201:enzymology / Q000235:genetics; D004722:Endoribonucleases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005095:Exoribonucleases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013696:Temperature
D000904:Antibiotics, Antitubercular; D003574:Cytochrome c Group; D012329:RNA, Bacterial; D012333:RNA, Messenger; C017165:cytochrome c(3); D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D005095:Exoribonucleases; C022535:exoribonuclease II; D015719:Single-Strand Specific DNA and RNA Endonucleases; C022537:ribonuclease E; D012293:Rifampin