title: Activation of an anion conductance and beta-cell depolarization during hypotonically induced insulin release.
abstract: The perforated patch technique was used to study the effects of hypotonic extracellular solutions on membrane potential and whole-cell currents in intact rat pancreatic beta-cells. A 30% reduction in osmolarity resulted in activation of an outwardly rectifying CI(-)-selective conductance in rat beta-cells. This conductance was inhibited by the anion channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS). Exposure to a hypotonic medium also led to a transient stimulation of electrical activity accompanied by cell swelling and a gradual return towards control volume. These effects were also associated with the generation of an inward current at a holding potential of -70mV, and a stimulation of insulin release from intact perifused islets. All of the above effects were inhibited by DIDS. It is suggested that the stimulation of insulin release by hypotonic solutions results from activation of a volume-sensitive anion conductance generating an inward current and leading to a subsequent depolarization of the beta-cell.
authors.affiliation: Department of Medicine and Biological Sciences, University of Manchester, UK.
authors.forename: L
authors.initials: L
authors.lastname: Best
authors.forename: H E
authors.initials: HE
authors.lastname: Miley
authors.forename: A P
authors.initials: AP
authors.lastname: Yates
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity; D005260:Female; D007038:Hypotonic Solutions / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007038:Hypotonic Solutions; D007328:Insulin; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid