Isolation, overexpression, and biochemical characterization of the two isoforms of glutamic acid decarboxylase from Escherichia coli.
Escherichia coli glutamic acid decarboxylase is a pyridoxal phosphate-dependent enzyme that catalyzes the alpha-decarboxylation of glutamate to yield 4-amino-butyrate and CO2. The E. coli chromosome contains two genes encoding for this enzyme, gadA and gadB, which map at distinct loci. Their protein products differ in only five amino acid residues, four of which are located in the N-terminal region (Smith et al., 1992, J. Bacteriol. 174, 5820-5826). Herein, we report the sequences of the two gad genes, including their regulatory regions. Both genes were separately cloned into the vector pQE60, for overexpression under the control of the lac promoter. In this way, we have succeded in separately expression large quantities of each pure isoform. The two isoforms were characterized biochemically and all evidence, including that from analysis of the complex pre-steady-state kinetic behavior of the enzymes, indicates that the functional properties of the two isoenzymes are identical.
De Biase
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Dipartimento di Scienze Biochemiche A. Rossi Fanelli, Università La Sapienza, Roma, Italy. debiase@caspur.it
Tramonti
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John
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Bossa
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D001483:Base Sequence; D015152:Blotting, Northern; D004926:Escherichia coli; D015971:Gene Expression Regulation, Enzymologic; D005968:Glutamate Decarboxylase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D007700:Kinetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
D005968:Glutamate Decarboxylase