Glycerol 3-phosphate dehydrogenase of boar spermatozoa: inhibition by alpha-bromohydrin phosphate.
Boar spermatozoa incubated with glycerol 3-phosphate as substrate produced CO2 and an accumulation of dihydroxyacetone phosphate and fructose-1,6-bisphosphate. The rate of oxidation of glycerol 3-phosphate was decreased, as was the production of CO2 and the two glycolytic intermediates, in the presence of (R,S)-alpha-bromohydrin phosphate. In the presence of inhibitors of stage two of the glycolytic pathway, CO2 production was prevented, there was a marked increase in the concentration of the glycolytic intermediates but the rate of metabolism of the substrate was unaffected. Oxygen consumption by spermatoza incubated with glycerol 3-phosphate was unaffected in the presence of rotenone, whereas it was decreased when lactate was offered as the substrate. The results reported here confirm that in boar spermatozoa glycerol 3-phosphate dehydrogenase is an FAD-linked enzyme that is inhibited by (R,S)-alpha-bromohydrin phosphate in, possibly, a competitive manner.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Gillan
L
L
D000818:Animals; D002478:Cells, Cultured; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology*; D012402:Rotenone / Q000494:pharmacology; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology
D005994:Glycerophosphates; D010710:Phosphates; D011409:Propylene Glycols; D014475:Uncoupling Agents; D012402:Rotenone; C006818:alpha-bromohydrin; D005993:Glycerolphosphate Dehydrogenase