In situ hybridization analysis of substance P receptor in the rat retina.
Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost their hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified the hybridization message as distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry.
Kondoh
A
A
Department of Anatomy, Kansai Medical University, Osaka, Japan.
Houtani
T
T
Ueyama
T
T
Baba
K
K
Ikeda
M
M
Yamagishi
K
K
Miki
H
H
Uyama
M
M
Nakanishi
S
S
Sugimoto
T
T
D000818:Animals; D017403:In Situ Hybridization; D008297:Male; D009409:Nerve Crush; D009900:Optic Nerve; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism
D018040:Receptors, Neurokinin-1