Disruption of the hslU gene, which encodes an ATPase subunit of the eukaryotic 26S proteasome homolog in Escherichia coli, suppresses the temperature-sensitive dnaA46 mutation.
The Escherichia coli hslVU genes encode subunits of an ATP-dependent protease, a homolog of the eukaryotic 26S proteasome. We found that the hslU gene is required for the temperature (40 degrees C)-sensitive phenotype of the dnaA46 mutant, while other soluble ATP-dependent proteases, La (Lon) and Ti (Clp), were unrelated to this dnaA46 phenotype. Disruption of the hslU gene inhibited cell growth at high temperatures. These observations suggest a specific in vivo role for HslVU protease in denatured proteins. As the absence of HslU produces minicells in M9 medium, the protease may be involved in the cell cycle regulation.
Katayama
T
T
Department of Microbiology, Kyushu University Faculty of Pharmaceutical Sciences, Fukuoka, Japan.
Kubota
T
T
Takata
M
M
Akimitsu
N
N
Sekimizu
K
K
D049069:ATP-Dependent Proteases; D000251:Adenosine Triphosphatases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002453:Cell Cycle; D003546:Cysteine Endopeptidases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D005057:Eukaryotic Cells; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D009097:Multienzyme Complexes / Q000235:genetics; D009154:Mutation; D049070:Protease La; D046988:Proteasome Endopeptidase Complex; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012697:Serine Endopeptidases / Q000235:genetics; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D009097:Multienzyme Complexes; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La; D049071:Endopeptidase Clp; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases