PEG thiazolidine-2-thione, a novel reagent for facile protein modification: conjugation of bovine hemoglobin.
A novel PEG linker that employs a thiazolidine-2-thione group has been synthesized. Kinetic studies done on this compound demonstrate a relatively long half-life compared to those of traditional succinimidyl linkers. This new PEG derivative reacts with proteins under mild conditions and was utilized to conjugate bovine hemoglobin (bHb) to provide a PEG amide-linked protein. The physical characteristics of this conjugate were compared with those of the known PEG carbamate-linked bHb.
Greenwald
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Department of Medicinal Chemistry, Enzon Inc., Piscataway, New Jersey 08854-3969, USA.
Pendri
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Martinez
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Gilbert
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C
Bradley
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P
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000737:chemistry*; D007202:Indicators and Reagents; D011092:Polyethylene Glycols; D013844:Thiazoles / Q000737:chemistry*; D053778:Thiazolidines
D006454:Hemoglobins; D007202:Indicators and Reagents; D013844:Thiazoles; D053778:Thiazolidines; D011092:Polyethylene Glycols; C005104:2-mercaptothiazoline