title: A cost effective base-matching assay with low backgrounds.
abstract: Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification. However, its application is often hampered by high background and high cost. We have decreased the background by approximately 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.
authors.affiliation: Theobald Smith Research Institute, Inc., The New England Regional Newborn Screening Program, Boston, MA 02130, USA. xsu@world.std.com
authors.forename: X
authors.initials: X
authors.lastname: Su
authors.forename: G
authors.initials: G
authors.lastname: Mushinsky
authors.forename: A M
authors.initials: AM
authors.lastname: Comeau
mesh_terms: D005838:Genotype; D005914:Globins / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000191:economics* / Q000379:methods
chemical_list: D005914:Globins