Design, production and characterization of antibodies discriminating between the phenol- and monoamine-sulphating forms of human phenol sulphotransferase.
1. Phenol sulphotransferases (PSTs) are important enzymes in xenobiotic and endobiotic detoxication, and a key component of the body's chemical defence mechanism. 2. Human phenol-(P-PST) and monoamine-(M-PST) sulphating forms of PST share 93% amino acid sequence identity, and to date the various antibodies produced against PSTs all recognize both enzymes. 3. We have identified two peptides based on the cDNA-derived amino acid sequences of human P-PST and M-PST, which elicited for the first time antibodies capable of discriminating between these highly homologous enzymes. 4. These antibodies represent valuable tools for studying the expression, distribution and function of human phenol sulphotransferases.
Rubin
G L
GL
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.
Sharp
S
S
Jones
A L
AL
Glatt
H
H
Mills
J A
JA
Coughtrie
M W
MW
D000595:Amino Acid Sequence; D000906:Antibodies / Q000032:analysis*; D015239:Arylsulfotransferase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*
D000906:Antibodies; D010455:Peptides; D015239:Arylsulfotransferase