title: Genetic mapping and chromosome localization of the rat mitochondrial glycerol-3-phosphate dehydrogenase gene, a candidate for non-insulin-dependent diabetes mellitus.
abstract: The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mtGPD) plays an important role in the regulation of insulin secretion and has been postulated as a candidate responsible for the pathogenesis of non-insulin-dependent diabetes mellitus (NIDDM) in humans as well as in rodent models of NIDDM. Recent molecular genetic studies of the Goto-Kakizaki (GK) rat model of NIDDM have identified loci linked to NIDDM. To elucidate whether rat mtGPD might play a role in the pathogenesis of NIDDM, the rat mtGPD gene (Gpd2) was cloned, and a genetic marker for Gpd2 was developed. The gene mapped to the region of rat chromosome 3 that contains a region linked to NIDDM in the GK rat. Fluorescence in situ hybridization was also carried out to verify the map position.
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital-East, Charlestown 02129-2060, USA. koike@post.its.mcw.edu
authors.forename: G
authors.initials: G
authors.lastname: Koike
authors.forename: P
authors.initials: P
authors.lastname: Van Vooren
authors.forename: M
authors.initials: M
authors.lastname: Shiozawa
authors.forename: J
authors.initials: J
authors.lastname: Galli
authors.forename: L S
authors.initials: LS
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Glaser
authors.forename: A
authors.initials: A
authors.lastname: Balasubramanyam
authors.forename: L J
authors.initials: LJ
authors.lastname: Brown
authors.forename: H
authors.initials: H
authors.lastname: Luthman
authors.forename: C
authors.initials: C
authors.lastname: Szpirer
authors.forename: M J
authors.initials: MJ
authors.lastname: MacDonald
authors.forename: H J
authors.initials: HJ
authors.lastname: Jacob
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004195:Disease Models, Animal; D008040:Genetic Linkage; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping
chemical_list: D005993:Glycerolphosphate Dehydrogenase