Agonistic effects of anti-peptide antibodies and autoantibodies directed against adrenergic and cholinergic receptors: absence of desensitization.
Affinity-purified autoantibodies and anti-peptide antibodies directed against the second extracellular loop of the beta 1-adrenoceptor increase the beating rate of cultured cardiomyocytes just like the beta-adrenergic agonist isoprenaline. Their positive chronotropic action is blocked by beta-adrenergic antagonists. Affinity-purified autoantibodies and anti-peptide antibodies directed against the muscarinic cholinergic M2 receptor exert in these myocytes, like the muscarinic cholinergic agent carbachol, a negative chronotropic effect that is antagonized by atropine. In contrast to the agonism of isoprenaline and carbachol, the described agonistic effects of the antibodies are not subject to desensitization.
Wallukat
G
G
Max-Delbrück-Centre for Molecular Medicine, Berlin, Germany.
Fu
M L
ML
Magnusson
Y
Y
Hjalmarson
A
A
Hoebeke
J
J
Wollenberger
A
A
D000318:Adrenergic beta-Agonists / Q000276:immunology* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D001323:Autoantibodies / Q000276:immunology / Q000494:pharmacology*; D002478:Cells, Cultured; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D043585:Receptor, Muscarinic M2; D011943:Receptors, Adrenergic, beta / Q000276:immunology*; D011976:Receptors, Muscarinic / Q000276:immunology*
D000318:Adrenergic beta-Agonists; D000906:Antibodies; D001323:Autoantibodies; D010455:Peptides; D043585:Receptor, Muscarinic M2; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic