Intracellular Ca2+ mobilization in cultured human mucosal microvascular endothelial cells from the nasal inferior turbinate.
Human mucosal microvascular endothelial cells (HMMECs) were isolated and cultured from the mucosa of nasal inferior turbinates. These cells were identified by human factor-VIII antigen and UEA-I. We also investigated alterations in intracellular calcium concentration [Ca2+]i, in HMMECs using the Ca(2+)-sensitive dye fura-2. Depolarization by high K+ (150 mM) induced no changes in [Ca2+]i in HMMECs, thereby indicating the lack of voltage-dependent Ca2+ channels in these cells. Extracellular ATP increased [Ca2+]i in HMMECs both in the presence and absence of extracellular Ca2+. These results suggest that ATP may induce a release of Ca2+ from intracellular stores in HMMECs. Adenosine induced no changes in [Ca2+]i in HMMECs. It is thus likely that HMMECs obtained from the nasal inferior turbinate may carry P2-purinoceptors but not P1-purinoceptors.
Ikeda
H
H
Department of Otorhinolaryngology, Kansai Medical University, Osaka, Japan.
Kubo
N
N
Nakamura
A
A
Harada
N
N
Yamashita
T
T
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes; D016257:Fura-2; D006651:Histocytochemistry; D006801:Humans; D008856:Microscopy, Fluorescence; D009297:Nasal Mucosa / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000378:metabolism; D014420:Turbinates / Q000098:blood supply / Q000378:metabolism*
D005456:Fluorescent Dyes; D011983:Receptors, Purinergic; D002118:Calcium; D016257:Fura-2