Expression of prolactin receptors in human osteosarcoma cells.
The expression of prolactin receptor (PRL-R) mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) combined with Southern analysis in total RNA extracts from two human osteosarcoma cell lines (MG-63 and Saos-2). The level of PRL-R transcript was significantly enhanced in cells cultured in the presence of 1,25-(OH)2 vitaminD3 (10(-7)M) and to a lesser extent in the presence of dexamethasone (10(-6)M). This first demonstration of PRL-R gene expression in osteoblast-like cells supports the hypothesis of a direct action of prolactin in bone cells, which is further strongly suggested by the stimulatory effect of 1,25-(OH)2 vitaminD3 and dexamethasone on PRL-R mRNA level in these cells.
Bataille-Simoneau
N
N
Laboratoire d'Histologie-Embryologie (LHEA), UFR Médecine, Angers, France.
Gerland
K
K
Chappard
D
D
Basle
M F
MF
Mercier
L
L
D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012516:Osteosarcoma; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D011981:Receptors, Prolactin / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011981:Receptors, Prolactin; D003907:Dexamethasone; D002117:Calcitriol