Chromatographic and electrophoretic applications for the analysis of heparin and dermatan sulfate.
Heparin and dermatan sulfate are highly sulfated polydisperse glycosaminoglycans. The methods to determine such compounds include chromatographic and electrophoretic techniques. Here we report on the performances of various analytical methods for the characterization and the determination of GAGs. Heparin, low-molecular-mass heparins, dermatan sulfate and low-molecular-mass dermatan sulfate were analyzed. High-performance size exclusion chromatography was used to determine the molecular mass, polydispersity, absorbance and the area under the absorbance-time curve. Polyacrylamide gel electrophoresis was used to determine the average molecular mass and the polydispersity. Heparin and dermatan sulfate preparations were analyzed by capillary electrophoresis using reversed polarity. The results obtained reflect different performances of various analytical methods used to characterize GAGs.
Malsch
R
R
1st Department of Medicine, Medical University Clinic Mannheim, Germany.
Harenberg
J
J
Piazolo
L
L
Huhle
G
G
Heene
D L
DL
D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D017985:Dalteparin / Q000032:analysis / Q000737:chemistry; D003871:Dermatan Sulfate / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006493:Heparin / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet
D003871:Dermatan Sulfate; D006493:Heparin; D017985:Dalteparin