title: Neuronal nicotinic receptor alpha 6 subunit mRNA is selectively concentrated in catecholaminergic nuclei of the rat brain.
abstract: Although the neuronal nicotinic receptor alpha 6 subunit was cloned several years ago, its functional significance remains to be investigated. Here we describe an in situ hybridization study of the mRNA for this subunit in the adult rat central nervous system using oligonucleotide probes. Specific alpha 6 mRNA labelling was restricted to a few nuclei throughout the brain; it was particularly high in several catecholaminergic nuclei [the locus coeruleus (A6), the ventral tegmental area (A10) and the substantia nigra (A9)] at levels significantly higher than those found for any other known nicotinic receptor subunit mRNA. Labelling for alpha 6 mRNA was also detected at lower levels in the reticular thalamic nucleus, the supramammillary nucleus and the mesencephalic V nucleus. Some cells of the medial habenula (medioventral part) and of the interpeduncular nucleus (central and lateral parts) were also labelled. The distribution of alpha 6 mRNA was compared with the distribution of the other known nicotinic acetylcholine receptor subunit mRNAs. In several nuclei, the expression of alpha 6 was complementary to those of other alpha subunits. Moreover, some of the cell groups (such as the substantia nigra, the ventral tegmental area and the locus coeruleus) previously thought to contain mainly alpha 3 mRNA in fact were found to contain high levels of alpha 6 mRNA. Finally, we found extensive colocalization of alpha 6 and beta 3, indicating the possible existence of nicotinic receptor hetero-oligomers containing both subunits. The present results show that alpha 6 is the major nicotinic acetylcholine receptor alpha subunit expressed in dopaminergic cell groups of the mesencephalon and noradrenergic cells of the locus coeruleus. This suggests the involvement of the alpha 6 subunit in some of the major functions of central nicotinic circuits, including the modulation of locomotor behaviour and reward.
authors.affiliation: CNRS URA 1284, Pasteur Institute, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Le Novère
authors.forename: M
authors.initials: M
authors.lastname: Zoli
authors.forename: J P
authors.initials: JP
authors.lastname: Changeux
mesh_terms: D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000096:biosynthesis*; D013378:Substantia Nigra / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013681:Tegmentum Mesencephali / Q000378:metabolism; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D003838:Deoxyadenine Nucleotides; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D013462:Sulfur Radioisotopes; C104518:nicotinic receptor alpha6; D014446:Tyrosine 3-Monooxygenase; C026600:2'-deoxyadenosine triphosphate