title: New class of poly(vinyl alcohol) polymers as column-chromatography stationary phases for Candida rugosa lipase isoforms separation.
abstract: The preparation of new stationary phases of cross-linked polyvinyl alcohol esterified with various linear fatty acids is described. The physico-chemical properties of these polymers are reported, including electron microscopy and swelling measurements. Batch adsorption experiments were performed in order to characterize the basic separative properties of these phases. Cross-linked polyvinyl alcohol esterified with dodecanoic acid was used for hydrophobic interaction chromatography of a commercial crude preparation of Candida rugosa lipase. Characterization of the purified fractions was carried out via native electrophoresis and sodium dodecyl sulfate-polyacrylamide electrophoresis.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Battinelli
authors.forename: E
authors.initials: E
authors.lastname: Cernia
authors.forename: M
authors.initials: M
authors.lastname: Delbò
authors.forename: G
authors.initials: G
authors.lastname: Ortaggi
authors.forename: A
authors.initials: A
authors.lastname: Pala
authors.forename: S
authors.initials: S
authors.lastname: Soro
mesh_terms: D002175:Candida / Q000201:enzymology*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D011108:Polymers; D011142:Polyvinyl Alcohol; D008049:Lipase