[Determination of total free erythrocyte carnitine in an ultrafiltrate of hemolysate].
The authors described an assay procedure for the determination of free and total carnitine in red blood cells. This method is based on ultrafiltration of the broken red blood cells in Centrisart filter, by centrifugation. Free and total carnitine in ultrafiltrates is determined by the spectrophotometric method of Cederblad and Coll., adapted to Cobas-Fara. They evaluated the procedure linearity, reproductability and analytical recovery. Comparison with precipitate protein procedure showed that results correlated well. The advantages of this original assay include simplicity, increase rapidity, lower cost of reagents and mainly improved reproductability.
Iordache
A
A
Laboratoire de Biochimie, Hôpital Pasteur, Nice, France.
Bayle
J
J
Chambon
P
P
D002331:Carnitine / Q000097:blood*; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000737:chemistry*; D006440:Hemofiltration / Q000379:methods*; D006461:Hemolysis; D066298:In Vitro Techniques; D013053:Spectrophotometry; D014462:Ultrafiltration
D002331:Carnitine
Dosage de la carnitine libre et totale érythrocytaire dans un ultrafiltrat d'hémolysat.