title: DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes.
abstract: We found that dimethyl-sulfoxide (DMSO) at concentrations of 2.5% induced apoptosis in SV40-immortalized human keratinocytes, while normal keratinocytes were arrested at the boundary of G1/S phase under the same conditions. DMSO-induced apoptosis in SV-40 immortalized keratinocytes was not associated with change in phosphorylated state of the retinoblastoma susceptibility gene. When SV40-immortalized cells were treated with 2.5% DMSO, dissociation of the complex was observed by immunoblotting of SV40 T antigen from immunoprecipitated p53 protein fraction.
authors.affiliation: Department of Cancer Cell Research, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Koike
authors.forename: K
authors.initials: K
authors.lastname: Ishino
authors.forename: Y
authors.initials: Y
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Tachikawa
authors.forename: T
authors.initials: T
authors.lastname: Kartasova
authors.forename: T
authors.initials: T
authors.lastname: Kuroki
authors.forename: N
authors.initials: N
authors.lastname: Huh
mesh_terms: D000957:Antigens, Viral, Tumor / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D016160:Retinoblastoma Protein / Q000378:metabolism; D013539:Simian virus 40 / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000957:Antigens, Viral, Tumor; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; D004121:Dimethyl Sulfoxide