title: In situ hybridization analysis of substance P receptor in the rat retina.
abstract: Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost their hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified the hybridization message as distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry.
authors.affiliation: Department of Anatomy, Kansai Medical University, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kondoh
authors.forename: T
authors.initials: T
authors.lastname: Houtani
authors.forename: T
authors.initials: T
authors.lastname: Ueyama
authors.forename: K
authors.initials: K
authors.lastname: Baba
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Yamagishi
authors.forename: H
authors.initials: H
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Uyama
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D017403:In Situ Hybridization; D008297:Male; D009409:Nerve Crush; D009900:Optic Nerve; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism
chemical_list: D018040:Receptors, Neurokinin-1