[Computer-assisted evaluation of a "medium resolution" HLA-DRB oligotyping of bone marrow donors].
One drawback of a 'medium resolution' generic dotblot oligonucleotide typing of the HLA-DRB locus has been the time consuming evaluation of the collected SSO-hybridisation datas. We developed a program on the basis of MS-ACCESS, written in visual basic, called HIDE (HLA Intelligent Data Evaluation) to manage this problem. HIDE offers a complete data management for the donor and the incoming sample, supports a membrane based data entry of the hybridisation-reactions and multiple testing of a sample. If an evaluation shows more than one matching allele combination, the result is summarized according to the main DRB-groups. The flexible structure of the program allows the entry of additional allelspecific reaction-patterns as well as the adaption to different SSO-typingsets.
Helmberg
W
W
Department für Transfusionsmedizin und Immunhämatologie, Chirurgische Universitäts-Klinik, LKH Graz, Osterreich.
Ulrich
S
S
Pätzold
U
U
Lanzer
G
G
D000483:Alleles; D001185:Artificial Intelligence; D016026:Bone Marrow Transplantation; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000295:instrumentation*; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation*; D009841:Oligonucleotides / Q000235:genetics; D012984:Software
D006684:HLA-DR Antigens; D009841:Oligonucleotides
Computerunterstützte Auswertung einer "Medium resolution"-HLA-DRB-Oligotypisierung von Knochenmarkspendern.