title: [Attenuation of type I restriction in Escherichia coli: effect of the ard gene in UV-irradiated cells].
abstract: The effect of conjugative plasmids ColIb-P9 (incI1) and pKM 101 (incN), containing the active ard gene, on the efficiency of EcoK restriction of nonmodified phage lambda .0 in UV-irradiated Escherichia coli cells was studied. ard-Dependent antirestriction enzyme activity was shown to decrease in UV-irradiated cells. The efficiency of action of the ard plasmid gene on lambda .0 was also shown not to depend on cell helicases RecBCD and UvrD, in contrast to the UV-induced alleviation of EcoK restriction (SOS alleviation).
authors.forename: G B
authors.initials: GB
authors.lastname: Zavil'gel'skiĭ
authors.forename: I V
authors.initials: IV
authors.lastname: Manukhov
authors.forename: S M
authors.initials: SM
authors.lastname: Rastorguev
mesh_terms: D015253:Deoxyribonucleases, Type I Site-Specific / Q000037:antagonists & inhibitors / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005798:Genes, Bacterial; D006868:Hydrolysis; D010957:Plasmids; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays
chemical_list: C029602:endodeoxyribonuclease EcoK; D015253:Deoxyribonucleases, Type I Site-Specific
vernacular_title: Oslablenie restriktsii I tipa u Escherichia coli: deĭstvie gena ard v UF-obluchennykh kletakh.