Genetic mapping and chromosome localization of the rat mitochondrial glycerol-3-phosphate dehydrogenase gene, a candidate for non-insulin-dependent diabetes mellitus.
The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mtGPD) plays an important role in the regulation of insulin secretion and has been postulated as a candidate responsible for the pathogenesis of non-insulin-dependent diabetes mellitus (NIDDM) in humans as well as in rodent models of NIDDM. Recent molecular genetic studies of the Goto-Kakizaki (GK) rat model of NIDDM have identified loci linked to NIDDM. To elucidate whether rat mtGPD might play a role in the pathogenesis of NIDDM, the rat mtGPD gene (Gpd2) was cloned, and a genetic marker for Gpd2 was developed. The gene mapped to the region of rat chromosome 3 that contains a region linked to NIDDM in the GK rat. Fluorescence in situ hybridization was also carried out to verify the map position.
Koike
G
G
Cardiovascular Research Center, Massachusetts General Hospital-East, Charlestown 02129-2060, USA. koike@post.its.mcw.edu
Van Vooren
P
P
Shiozawa
M
M
Galli
J
J
Li
L S
LS
Glaser
A
A
Balasubramanyam
A
A
Brown
L J
LJ
Luthman
H
H
Szpirer
C
C
MacDonald
M J
MJ
Jacob
H J
HJ
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004195:Disease Models, Animal; D008040:Genetic Linkage; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping
D005993:Glycerolphosphate Dehydrogenase