Acid phosphatase in the pathogenic and nonpathogenic hemoflagellates, Cryptobia spp., of fishes.
Acid phosphatase (ACP) was detected in whole-cell lysates, membrane-bound and water-soluble fractions of Cryptobia salmositica (pathogenic and nonpathogenic vaccine strains), Cryptobia bullocki, and Cryptobia catostomi using p-nitro-phenylphosphate as the substrate. High activities were in acidic pH (3.0-5.5) and the optimal pH was 5.0 Highest ACP activity was in the membrane-bound fraction. The pathogenic strain of C. salmositica had significantly higher total ACP activity than the vaccine strain and the other 2 species. However, the activity in the pathogenic C. salmositica decreased significantly with prolonged in vitro cultivation. The membrane-bound ACP of the pathogenic C. salmositica had highest resistance to the ACP inhibitor, sodium tartrate.
Zuo
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Department of Zoology, University of Guelph, Ontario, Canada.
Woo
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D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D003531:Cypriniformes / Q000469:parasitology; D005393:Fish Diseases / Q000469:parasitology*; D006863:Hydrogen-Ion Concentration; D016830:Kinetoplastida / Q000201:enzymology* / Q000472:pathogenicity; D017686:Oncorhynchus mykiss / Q000469:parasitology; D011528:Protozoan Infections / Q000469:parasitology; D011529:Protozoan Infections, Animal; D016052:Protozoan Vaccines; D012692:Serial Passage; D013644:Tartrates / Q000494:pharmacology; D013997:Time Factors
D016052:Protozoan Vaccines; D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid