title: [Detection of nordiazepam in the hair of drug addicts].
abstract: Hair samples were obtained from 36 drug addicts deceased from fatal heroin overdose. Samples were decontaminated with dichloromethane and pulverized. 50 mg of powdered hair were incubated 2 h at 40 degrees C in a phosphate buffer pH 7.6 in presence of deuterated internal standards (Nordiazepam-d5 and Oxazepam-d5) and extracted by a mixture ether/chloroform (80:20, v/v) at pH 8.4. After evaporation of the solvent, the dry extract was derivatized by silylation (BSTFA + 1% TMCS) and analyzed by gas chromatography coupled to mass spectrometry, with a detector operating in negative chemical ionization mode. 13 samples tested positive for Nordiazepam with concentrations ranging from 0.25 to 18.87 ng/mg of hair (mean: 4.16 ng/mg) and 5 positives for its metabolite, Oxazepam, with concentrations ranging from 0.11 to 0.50 ng/mg of hair (mean : 0.28 ng/mg).
authors.affiliation: Institut de Médecine Légale, Strasbourg.
authors.forename: F
authors.initials: F
authors.lastname: Vayssette
authors.forename: V
authors.initials: V
authors.lastname: Cirimele
authors.forename: P
authors.initials: P
authors.lastname: Kintz
authors.forename: P
authors.initials: P
authors.lastname: Mangin
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006197:Hair / Q000737:chemistry*; D006556:Heroin Dependence / Q000378:metabolism*; D006801:Humans; D003708:Nordazepam / Q000032:analysis*; D010076:Oxazepam / Q000032:analysis
chemical_list: D003708:Nordazepam; D010076:Oxazepam
vernacular_title: Détection du Nordiazépam dans les cheveux de toxicomanes.