[The effect of coenzyme Q10 and cold cristalloid cardioplegia on hypothermic global ischemia].
Coenzyme Q10 (CoQ10, ubiquinone) has been shown to be protective against myocardial ischemia/reperfusion induced injury. The purpose of this study was to investigate the effect of CoQ10 added to cold cristalloid cardioplegia on hypothermic ischemia and normothermic reperfusion using an isolated working rat heart. Hearts (n = 6-9/group) from male Wistar rats were aerobically (37 degrees C) perfused (20 min) with bicarbonate buffer. This was followed by a 3-min infusion of St. Thomas' Hospital cardioplegic solution containing various concentrations of CoQ10 (0, 1, 3, 6, 12, and 58 mumol/L). Hearts were then subjected to 180 min of hypothermic (20 degrees C) global ischemia and 35 min of normothermic (37 degrees C) reperfusion (15 min Langendorff, 20 min working). Ventricular fibrillation (Vf) upon reperfusion was irreversible in the 12 and 58 mumol/ L CoQ10 groups (4/6 and 3/6, respectively). In the hearts which Vf upon reperfusion was not irreversible, the percent recovery of aortic flow (%AF) was 43.3 +/- 5.4% (n = 9) in the control group versus 31.6 +/- 7.7% (n = 6), 38.0 +/- 12.0% (n = 6), 27.2 +/- 6.9% (n = 6), 31.3% (n = 2), and 30.4 +/- 14.2% (n = 3) in the 1, 3, 6, 12, and 58 mumol/L CoQ10 groups, respectively. Creatine kinase leakage during Langendorff reperfusion tended to be greater in the 12 and 58 mumol/L CoQ10 groups than in the control group. Thus, CoQ10 in the cold cristalloid cardioplegic solution induced irreversible Vf upon reperfusion and failed to improve functional recoveries following hypothermic global ischemia.
Yamamoto
H
H
Department of Cardiovascular Surgery, National Cardiovascular Center, Osaka, Japan.
Yamamoto
F
F
D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D003067:Coenzymes; D005947:Glucose / Q000494:pharmacology; D006324:Heart Arrest, Induced; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology* / Q000628:therapy; D009206:Myocardium / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014325:Tromethamine / Q000494:pharmacology; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
D002314:Cardioplegic Solutions; D003067:Coenzymes; C074097:Krebs-Henseleit solution; D017680:Potassium Compounds; C018369:potassium cardioplegic solution; D014325:Tromethamine; D014451:Ubiquinone; C024989:coenzyme Q10; D005947:Glucose