title: pH-dependent actions of THIP and ZAPA on an ionotropic Drosophila melanogaster GABA receptor.
abstract: The actions of THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) and ZAPA (Z-3-[(aminoiminomethyl)thio]prop-2-enoic acid) were tested on an ionotropic homo-oligomeric GABA receptor of Drosophila melanogaster. The amplitude of currents activated by THIP and ZAPA declined rapidly during agonist application and a rebound response was observed on washout. By correcting the pH shift induced by these acid salts, responses more typical of GABA agonists were seen. Less striking pH-dependence was observed in the case of GABA responses.
authors.affiliation: Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, UK.
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: A M
authors.initials: AM
authors.lastname: Hosie
authors.forename: S D
authors.initials: SD
authors.lastname: Buckingham
authors.forename: M D
authors.initials: MD
authors.lastname: Squire
authors.forename: H A
authors.initials: HA
authors.lastname: Baylis
authors.forename: D B
authors.initials: DB
authors.lastname: Sattelle
mesh_terms: D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D004331:Drosophila melanogaster; D005260:Female; D018755:GABA Agonists / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007555:Isoxazoles / Q000494:pharmacology*; D009865:Oocytes / Q000378:metabolism; D018079:Receptors, GABA / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014981:Xenopus; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D000179:Acrylates; D018755:GABA Agonists; D007555:Isoxazoles; D018079:Receptors, GABA; D011994:Recombinant Proteins; D005680:gamma-Aminobutyric Acid; C058552:3-((aminoiminomethyl)thio)-2-propenoic acid; C015542:gaboxadol