An increase of acidic isoform of catalase in red blood cells from HIV(+) population.
A systemic oxidative stress of HIV (+) individuals has been recognized from a low glutathione level and a high level of inflammatory cytokines such as TNF alpha. Previously, we demonstrated that the catalase enzyme activity in HIV (+) population is significantly altered depending on the cell types; the level was significantly high in red blood cells while the enzymes in white blood cells were remarkably low (Res Commun Subs Abuse 16: 161-176, 1995). In this study, we further characterized the difference in RBC catalase molecules between HIV (+) and control population. We have found that RBC from HIV (+) population, whether they were asymptomatic or symptomatic, contained a significantly elevated catalase protein accompanied by the enzyme activities, and that the majority of the elevated protein were acidic pI of the molecules with an identical subunit mass of approximately 60 KDa. These results suggest that catalase is induced prior to and/or during erythroid differentiation lineage in HIV (+) population as a somatic defense to respond and compensate for a systemic oxidative stress and for an anemic condition.
Yano
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S
Department of Biochemistry, Ponce School of Medicine, Puerto Rico, USA.
Colon
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M
Yano
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D000328:Adult; D000368:Aged; D000740:Anemia / Q000150:complications / Q000378:metabolism; D015153:Blotting, Western; D002374:Catalase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D015658:HIV Infections / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D019966:Substance-Related Disorders
D007527:Isoenzymes; D002374:Catalase