Positional cloning of the Drosophila melanogaster mei-9 gene, the putative homolog of the Saccharomyces cerevisiae RAD1 gene.
Mutation of the Drosophila melanogaster mei-9 gene causes enhanced mutagen sensitivity, DNA excision repair incision deficiency, germ line hypermutability, mitotic chromosome breakage and instability, and, in females, increased levels of meiotic non-disjunction and decreased levels of meiotic recombination. This paper reports on the utilization of a positional mapping strategy combined with a differential expression assay to clone the mei-9 gene. We report that mei-9 is homologous to the yeast repair gene, RAD1, and demonstrate that the mei-9 gene transcript is absent in the mei-9AT3 mutant.
Araj
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Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
Smith
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D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004260:DNA Repair / Q000235:genetics*; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D012689:Sequence Homology, Nucleic Acid
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C097527:MEI-9 protein, Drosophila; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D004720:Endonucleases; C069266:RAD1 protein, S cerevisiae; C499354:rad1 protein, S pombe; D000251:Adenosine Triphosphatases; C075467:RAD16 protein, S cerevisiae; D045643:DNA Repair Enzymes