Heat-stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide.
The role of lipopolysaccharide (LPS) in the serotyping of Campylobacter jejuni based on heat-stable antigens was examined using SDS-PAGE and a silver stain for carbohydrate. None of the 32 type strains of Camp. jejuni expressed long-chain LPS. Rabbit antibodies, prepared to 10 selected strains of Camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not LPS. This study suggests that the heat-stable antigens of Camp. jejuni, which form the basis for the established Penner serotyping scheme, are probably capsular and not LPS.
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Laboratory of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, UK.
Frost
J A
JA
Oza
A
A
Thwaites
R
R
Gillanders
S
S
Rowe
B
B
D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000145:classification* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000276:immunology; D006388:Hemagglutinins; D006358:Hot Temperature; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D012703:Serotyping
D000942:Antigens, Bacterial; D006388:Hemagglutinins; D008070:Lipopolysaccharides