Localization of type-1 porin channel (VDAC) in the sarcoplasmatic reticulum.
Eucaryotic porin channels or voltage-dependent anion channels (VDACs) are expressed in the outer mitochondrial membranes and in the plasmalemma of mammalian cells. Subfractions of sarcoplasmatic reticulum (SR) obtained from rabbit skeletal muscle display type-1 porin channels in transverse tubuli (TT) when analysed by immunoblot analysis with type-1 porin specific monoclonal antibodies. These data are in agreement with our recent proposal suggesting the presence of porin channels in non-mitochondrial eucaryotic membranes.
Jürgens
L
L
Max-Planck-Institut für Experimentelle Medizin, Göttingen, Germany.
Kleineke
J
J
Brdiczka
D
D
Thinnes
F P
FP
Hilschmann
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008931:Mitochondria, Muscle / Q000378:metabolism / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D018272:Porins; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D050994:Voltage-Dependent Anion Channels
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007473:Ion Channels; D008565:Membrane Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels