[The effect of cytoskeleton modification on the process of trypsin-induced cell aggregation].
Studies were performed on the influence of cytoskeleton modification (by colchicine, vinblastine, and cytochalasin B, and by neoplastic transformation) on cell aggregation induced by small trypsin concentrations (the final concentrations equal to 1-100 mg/ml) added to a suspension of chick embryo fibroblasts dispersed from a monolayer with EDTA. It was shown that alteration of microfilaments resulted in the loss of the ability of plasma membranes to structural rearrangements. It is suggested that aggregation of these cells is realized due to a gradual exposure of adhesive sites along with proteolytic splitting of protein components of plasma membranes.
Nikol'skaia
V P
VP
Mazhul'
V M
VM
Konev
S V
SV
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003599:Cytoskeleton / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D051379:Mice; D008809:Mice, Inbred C3H; D014357:Trypsin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms
D014357:Trypsin
Vliianie modifikatsii tsikoskeletona na protsess tripsinindutsirovannoĭ agregatsii kletok.