title: Luminal non-selective cation and outwardly rectifying chloride channels in cultured strial marginal cells from gerbil.
abstract: Ionic channels located on the luminal side of strial marginal cells (MCs) of gerbil in culture were investigated using the patch-clamp technique. Two types of channels were identified. The most frequently recorded single-channel activity corresponded to a non-selective cation (NSC) channel with a conductance of 23.7 +/- 0.2 pS (n = 18) in symmetrical NaCl conditions. The channel was activated by internal Ca2+ and inhibited by internal adenine nucleotides and flufenamic acid. Spontaneous activity of NSC channels was found in 16% of the cell-attached patches and with a very high density (9 +/- 2 levels/patch, n = 28) in 100% of the excised patches. An outwardly rectifying chloride (ORC) channel was also identified in 14% of the patches but only after excision. The channel exhibited at 0 mV a unit conductance of 26.8 +/- 1.3 pS (n = 8) and a strong outward rectification in symmetrical NaCl conditions, and the open probability increased with depolarization. The luminal NSC channel and the ORC channel evidenced in this study might participate in the production of endolymph. Although extrapolation of the presents results to the in vivo situation should be made with caution, this study suggests that culture of strial MCs may be a suitable model for investigation of endolymph physiology.
authors.affiliation: Laboratoire d'ORL Expérimentale, Université Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Yeh
authors.forename: T
authors.initials: T
authors.lastname: Van den Abbeele
authors.forename: R
authors.initials: R
authors.lastname: Marianovski
authors.forename: P
authors.initials: P
authors.lastname: Herman
authors.forename: P
authors.initials: P
authors.lastname: Tran Ba Huy
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism*; D003051:Cochlea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004710:Endolymph / Q000187:drug effects / Q000502:physiology; D005439:Flufenamic Acid / Q000494:pharmacology; D005849:Gerbillinae; D008297:Male; D018408:Patch-Clamp Techniques; D013223:Statistics as Topic; D013316:Stria Vascularis / Q000166:cytology / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D018118:Chloride Channels; D005439:Flufenamic Acid; D002118:Calcium