title: Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen.
abstract: Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to oxygen limitation for the organism and likely for patients afflicted with the inherited autosomal disease cystic fibrosis.
authors.affiliation: Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Ohio 45257-0524, USA. hassetdj@ucbeh.san.uc.edu
authors.forename: D J
authors.initials: DJ
authors.lastname: Hassett
mesh_terms: D000464:Alginates / Q000378:metabolism* / Q000494:pharmacology; D000693:Anaerobiosis; D009566:Nitrates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000464:Alginates; D009566:Nitrates; D010100:Oxygen