title: [Mechanisms of metabolic changes after in-line filtration of erythrocytes].
abstract: BACKGROUND
  The reasons for the improved RBC storage after in-line filtration are not fully understood.
  
  
  METHODS
  Red blood cell concentrates were in-line filtered and stored for 6 weeks at 4 degrees C in SAGM. Parameter 2,3-DPG, p50, density, cell electrophoresis, pH were measured.
  
  
  CONCLUSIONS
  The obtained results may explain the metabolic findings: Cell retention (during filtration older, rigide RBC are retained in the filter- a relative increase of younger RBC); changes in RBC membrane permeability (a changed electrophoretic mobility is attributed to bound RBC membrane proteins to the filter material); blood cell depletion (a high depletion efficiency prevents toxic influences of degenerating/disintegrating WBC and PLT); release of substances from filter and blood bag material (during passage of anticoagulated blood some compounds may be released in ppm concentrations - the density of in-line filtered RBC is higher than unfiltered RBC after filtration procedure and hypothermic storage).
authors.affiliation: Institut für Transfusionsmedizin, Humboldt-Universität zu Berlin, Deutschland.
authors.forename: G
authors.initials: G
authors.lastname: Matthes
authors.forename: H
authors.initials: H
authors.lastname: Bäumler
authors.forename: K P
authors.initials: KP
authors.lastname: Krause
authors.forename: U
authors.initials: U
authors.lastname: Tofoté
authors.forename: W
authors.initials: W
authors.lastname: Knippel
authors.forename: A
authors.initials: A
authors.lastname: Budde
authors.forename: E
authors.initials: E
authors.lastname: Donath
authors.forename: H
authors.initials: H
authors.lastname: Kiesewetter
mesh_terms: D019794:2,3-Diphosphoglycerate; D001771:Blood Banks; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood; D017707:Erythrocyte Transfusion / Q000295:instrumentation*; D004912:Erythrocytes / Q000378:metabolism*; D006452:Hemoglobinometry; D006801:Humans; D008212:Lymphocyte Depletion / Q000295:instrumentation*; D009996:Osmotic Fragility / Q000502:physiology
chemical_list: D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate
vernacular_title: Mechanismen der metabolischen Veränderungen nach einer In-Line-Filtration von roten Blutzellen.