title: Purification and characterization of the eighth and ninth components of carp complement.
abstract: Complement components corresponding to mammalian C8 and C9 were isolated from carp (Cyprinus carpio) serum. Carp C8 (M(r) 146,000) proved to be a gamma-globulin composed of three polypeptide chains (alpha-chain, M(r) 62,000; beta-chain, M(r) 62,000; gamma-chain, M(r) 22,000). The alpha-chain was disulfide-linked to the gamma-chain and the beta-chain was non-covalently associated with the alpha-gamma chain, in fair agreement with mammalian C8. However, the N-terminal amino acid sequences of the three subunits showed no homology with those of human C8. Carp C9 was an alpha-globulin composed of a single polypeptide (M(r) 91,000) and the N-terminus was blocked. Carp serum depleted of C8 did not hemolyse either carp antibody-sensitized sheep erythrocytes or non-sensitized rabbit erythrocytes, while C9-depleted carp serum did not hemolyse the former, but did hemolyse the latter target cells, as in the case of C9-depleted human serum.
authors.affiliation: Laboratory of Marine Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Uemura
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: H
authors.initials: H
authors.lastname: Shiraishi
authors.forename: M
authors.initials: M
authors.lastname: Nakao
mesh_terms: D000818:Animals; D002347:Carps / Q000276:immunology*; D003185:Complement C8 / Q000737:chemistry* / Q000172:deficiency / Q000302:isolation & purification*; D003186:Complement C9 / Q000737:chemistry* / Q000172:deficiency / Q000302:isolation & purification*; D006461:Hemolysis / Q000276:immunology
chemical_list: D003185:Complement C8; D003186:Complement C9