Nicotine does not modulate IL-4 and interferon-gamma release from peripheral blood mononuclear cells and T cell clones activated by phorbol myristate acetate and calcium ionophore.
Tobacco smoking induces an increased nonspecific IgE response. IgE synthesis is controlled by IL-4 and IFN-gamma. The effect of nicotine (10(-10) to 10(-5) M), one of the major components of tobacco smoke, were studied on IL-4 and IFN-gamma release by peripheral blood mononuclear cells from 12 allergic patients and 12 nonallergic subjects and 16 T cell clones stimulated by nonspecific agonists (phorbol myristate acetate and calcium ionophore). The release of IL-4 and IFN-gamma was measured by ELISA in supernatants after a 48-hour culture. Nicotine did not modify IL-4 and IFN-gamma release by peripheral blood mononuclear cells or T cell clones. The effects of tobacco smoke on IgE production are unlikely to change in the T cell phenotype by nicotine.
Le Cam
L
L
INSERM U.454, Hôpital Arnaud de Villeneuve, Montpellier, France.
Lagier
B
B
Bousquet
J
J
Pène
J
J
D000001:Calcimycin / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation; D009538:Nicotine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D007476:Ionophores; D015847:Interleukin-4; D007073:Immunoglobulin E; D000001:Calcimycin; D009538:Nicotine; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate