Age dependence of NMDA receptor involvement in epileptiform activity in rat hippocampal slices.
The pattern of epileptiform activity recorded from a number of in vitro seizure models is age dependent: ictal discharges are observed in immature brain slices while interictal bursts are seen in adult brain slices. This study evaluated the involvement of the N-methyl-D-aspartate (NMDA) receptor in the age-dependency of epileptiform activity recorded in area CA1 of hippocampal slices in Mg(2+)-free medium. Incubation in Mg(2+)-free medium induced ictal activity in 84% of hippocampal slices from immature rats (postnatal 10-15 days). In contrast, adult slices responded with interictal bursting, while ictal activity was rare (9%). Bath application of the NMDA receptor antagonist D,L-2-amino-5-phosphonovaleric acid (DL-APV, 20 microM) converted ictal activity to interictal activity in the hippocampal slices from immature rats. In adult slices, bath application of NMDA (10-20 microM) in Mg(2+)-free medium induced ictal-like discharges. Perfusion with NMDA (20 microM) in a medium containing 1.5 mM Mg2+ induced ictal activity in immature slices while it evoked only interictal bursts in adult slices. These results suggest that differences in NMDA receptor function may be involved in the age-dependency of epileptiform activity induced by Mg(2+)-free medium. Enhanced NMDA receptor-mediated activity may partially underlie increased seizure susceptibility in the immature brain.
Wang
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Department of Neurology, Children's Hospital, Boston, Massachusetts 02115, USA.
Jensen
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D000367:Age Factors; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004827:Epilepsy / Q000503:physiopathology*; D006624:Hippocampus / Q000503:physiopathology*; D016202:N-Methylaspartate / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*
D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate