Priming of monocyte respiratory burst by beta-amyloid fragment (25-35).
In the present study we have examined the ability of the beta-amyloid peptide (A beta(25-35)) to modulate the respiratory burst activity of human monocytes in vitro. Incubation of the cells for 24 h with A beta(25-35) as well as with A beta(1-42) resulted in an enhanced production of reactive oxygen radicals (ROI) in response to phorbol 12-myristate 13-acetate (PMA). Such effect was additively increased by coincubation with interferon-gamma (IFN gamma), and was paralleled by modulation of gene and protein expression of some components of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system. Since the effects of A beta(25-35) were also reproduced in primary rat microglia, our findings indicate that A beta(25-35) can potentiate the ability of mononuclear phagocytes to produce ROI, and add further insights into its biological effects.
Meda
L
L
Institute of General Pathology, University of Verona, Italy.
Bonaiuto
C
C
Baron
P
P
Otvos
L
L
Rossi
F
F
Cassatella
M A
MA
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D006706:Homeostasis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D017628:Microglia / Q000187:drug effects / Q000502:physiology; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology*; D019255:NADPH Oxidases / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C075222:amyloid beta-protein (1-42); C074636:amyloid beta-protein (25-35); D007371:Interferon-gamma; D019255:NADPH Oxidases