Neuronal pathways involved in abdominal surgery-induced gastric ileus in rats.
The 20-min rate of gastric emptying of a noncaloric solution and c-fos expression detected by immunohistochemistry in the brain were monitored 3 h after abdominal surgery performed under 10-min enflurane anesthesia in rats. Abdominal surgery (laparotomy and 1-min manipulation of the cecum) decreased gastric emptying from 60.8 +/- 3.4 to 25.9 +/- 3.4%. Capsaicin applied to the celiac/superior mesenteric ganglia 2 wk before the experiment reduced the delay in gastric emptying induced by abdominal surgery (46.3 +/- 3.4%), whereas perivagal capsaicin application had no effect (23.6 +/- 7.9%). The corticotropin-releasing factor (CRF) antagonist [D-Phe12, Nle21,38,C alpha MeLeu37]CRF-(12--41) injected intracisternally (10-20 micrograms) prevented postoperative gastroparesis induced by surgery, while having no effect on basal gastric emptying. Abdominal surgery increased the number of Fos-positive cells in brain nuclei regulating autonomic outflow: the nucleus of the solitary tract, locus ceruleus, paraventricular nucleus, and supraoptic nucleus of the hypothalamus. These data indicate that capsaicin-sensitive splanchnic afferent fibers and activation of CRF receptors in the brain are part of the neuronal circuitry mediating gastric stasis 3 h after abdominal surgery.
Barquist
E
E
Center for Ulcer Research and Education/Digestive Disease Research Center, West Los Angeles Veterans Affairs Medical Center, California, USA.
Bonaz
B
B
Martinez
V
V
Rivier
J
J
Zinner
M J
MJ
Taché
Y
Y
D000005:Abdomen / Q000601:surgery*; D000287:Administration, Topical; D000818:Animals; D001921:Brain / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D002946:Cisterna Magna; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D005728:Ganglia, Sympathetic; D005746:Gastric Emptying / Q000187:drug effects; D018589:Gastroparesis / Q000517:prevention & control; D007150:Immunohistochemistry; D007267:Injections; D008297:Male; D009434:Neural Pathways / Q000502:physiology; D011183:Postoperative Complications; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D016760:Proto-Oncogene Proteins c-fos; D003346:Corticotropin-Releasing Hormone; D002211:Capsaicin