Conserved circadian elements in phylogenetically diverse algae.
Circadian expression of the luciferin-binding protein (LBP) from the dinoflagellate Gonyaulax polyedra is regulated at the translational level. A small interval in the lbp 3'-untranslated region, which contains seven UG-repeats, serves as a cis-acting element to which a trans-acting factor (CCTR) binds in a circadian manner. Its binding activity correlates negatively with the circadian expression of LBP. Here I report the identification of a protein in the green alga Chlamydomonas reinhardtii that represents a CCTR analog. It binds both specifically and under control of the circadian clock to the UG-repeat region. The data show for the first time that circadian cis-elements implicated in translational regulation have been conserved during evolution.
Mittag
M
M
Botanisches Institut, Ludwig-Maximilians-Universität München, Germany. mittag@botanik.biologie.uni-muenchen.de
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000502:physiology*; D002940:Circadian Rhythm; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000302:isolation & purification* / Q000502:physiology; D012091:Repetitive Sequences, Nucleic Acid
D016601:RNA-Binding Proteins