title: Cucumber mosaic virus from Ixora: infectious RNA transcripts confirm deficiency in satellite support and unusual symptomatology.
abstract: A cucumber mosaic virus isolate from an Ixora plant originating in the Philippines and first described 20 years ago (CMV-Ix) by Waterworth and Povish differs from other characterized CMV strains in its anomalous satellite support and in several biological and molecular properties. We describe the preparation of infectious transcripts from cloned complementary DNA and the characterization of progeny virus. The results confirm that CMV-Ix has a more limited host range than most CMV isolates. Virions of CMV-Ix are smaller than those of the control CMV-S, independent of the type of negative stain used. Furthermore, CMV-Ix from transcripts supports the replication of the satellite T-CARNA 5 but not of D-CARNA 5.
authors.affiliation: Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Tousignant
authors.forename: P M
authors.initials: PM
authors.lastname: McGarvey
authors.forename: P
authors.initials: P
authors.lastname: Piazzolla
authors.forename: J M
authors.initials: JM
authors.lastname: Kaper
mesh_terms: D003001:Cloning, Molecular; D019401:Cucumber Mosaic Virus Satellite / Q000235:genetics* / Q000502:physiology; D017799:Cucumovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D018076:DNA, Complementary; D009696:Nucleic Acids / Q000032:analysis; D010944:Plants / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000502:physiology
chemical_list: D019401:Cucumber Mosaic Virus Satellite; D018076:DNA, Complementary; D009696:Nucleic Acids; D012333:RNA, Messenger; D012367:RNA, Viral