title: Accessory cell requirements for T lymphocyte activation and interferon-gamma production in peripheral lymph nodes.
abstract: Allergen-activated draining lymph node cells (LNC) isolated from mice exposed topically to the contact allergen oxazolone mount vigorous proliferative responses and secrete substantial amounts of interferon-gamma (IFN-gamma) when cultured with the T lymphocyte mitogen concanavalin A (con A). In contrast, although naive LNC prepared from untreated mice display con A-driven proliferative responses of comparable magnitude, they produce only very low levels of IFN-gamma. Secretion of IFN-gamma by con A-stimulated naive LNC was augmented significantly by the addition to culture of a small number of syngeneic dendritic cells (DC), under conditions where there was no influence on the vigour of proliferative responses and where the exogenous DC themselves failed to produce IFN-gamma. Augmentation of IFN-gamma production was not observed when exogenous populations depleted of DC were added to culture. It is proposed that discrete aspects of the primary activation of naive T lymphocytes display differential requirements for accessory cells and that the development of IFN-gamma producing cells necessitates sufficient numbers of dendritic cells.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Hodgson
authors.forename: R J
authors.initials: RJ
authors.lastname: Dearman
authors.forename: A G
authors.initials: AG
authors.lastname: Morris
authors.forename: I
authors.initials: I
authors.lastname: Kimber
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*
chemical_list: D003208:Concanavalin A; D010081:Oxazolone; D007371:Interferon-gamma