The effect of sodium hyaluronate on the growth of rabbit corneal epithelial cells in vitro.
The effect of sodium hyaluronate (Na-HA) on the growth of rabbit corneal epithelial cells was investigated in vitro by using MTT assay and [3H]-thymidine incorporation. Na-HA, with a molecular weight of 86 x 10(4), enhanced the growth of the corneal epithelial cells cultured on a fibronectin (FN) coated dish, but when cells were cultured on a noncoated dish, Na-HA did not enhance the growth of the cells. Na-HA, labeled with 5-aminofluorescein (FA-HA), bound to the corneal epithelial cells, and the CD44-like molecule was identified on the cells by using a monoclonal antibody, OS/37. Furthermore, the binding of FA-HA to the cells was significantly inhibited by the monoclonal antibody. These results suggest that Na-HA binds to the CD44-like molecule associated with FN and then enhances the growth of the corneal epithelial cells.
Miyazaki
T
T
Tokyo Research Institute, Seikagaku Corporation, Japan.
Miyauchi
S
S
Nakamura
T
T
Takeshita
S
S
Horie
K
K
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003315:Cornea / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism
D004396:Coloring Agents; D018960:Hyaluronan Receptors; D013778:Tetrazolium Salts; D013844:Thiazoles; D006820:Hyaluronic Acid; D004247:DNA; C022616:thiazolyl blue; D013936:Thymidine