title: Brain mast cell degranulation regulates blood-brain barrier.
abstract: Mast cells synthesize vasoactive agents and a number of neurotransmitters. They are particularly numerous in the medial habenular region of the epithalamus, the attachment site of the choroid plexus. The present study examined whether degranulation of brain mast cells alters the permeability of the blood-brain barrier (BBB). To this end, doves were injected intramuscularly with the mast cell degranulator, compound 48/80 (C40/80), followed by i.v. injection of Evans blue. The distribution of the dye in the parenchyma was examined using digital imaging. Three brain areas were analyzed: the medial habenula (which also contains mast cells), the paraventricular nucleus (PVN, which abuts the third ventricle, but has no mast cells), and the lateral septal organ (LSO, a circumventricular organ with fenestrated capillaries). Significantly more Evans blue tracer and fewer toluidine blue-positive mast cells were detected in the medial habenula of subjects treated with C48/80 compared to saline controls. Evans blue did not enter the PVN in either the experimental or control group, while it entered the LSO equally in both. Degranulation of mast cells after C48/80 treatment was confirmed histochemically and ultrastructurally. The results support the hypothesis that brain mast cell degranulation locally alters BBB permeability. Activation of brain mast cells may provide a mechanism for regulated opening of the BBB.
authors.affiliation: Department of Psychology, Columbia University, New York, New York 10032, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhuang
authors.forename: A J
authors.initials: AJ
authors.lastname: Silverman
authors.forename: R
authors.initials: R
authors.lastname: Silver
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000502:physiology*; D010856:Columbidae; D005070:Evans Blue / Q000493:pharmacokinetics; D005260:Female; D007273:Injections, Intramuscular; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009928:Organ Specificity; D014018:Tissue Distribution; D003189:p-Methoxy-N-methylphenethylamine / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D005070:Evans Blue