Neuronal and non-neuronal localization of acid sulfated glycosaminoglycans (sGAG) and chondroitin proteoglycans in the rat medulla oblongata.
Acid sulfated glycosaminoglycans (sGAG) and chondroitin proteoglycans have been biochemically and immunohistochemically demonstrated in certain cortical areas of the CNS. Such molecules display an important role in cerebral function, modulating cell adhesion, migration and signal neuromediation. In the present study we have evidenced the presence of sGAG using the colloidal iron method and C0S, C4S- and C6S-proteoglycans by the immunohistochemical pre-embedding PAP method. Our results have demonstrated the presence of such molecules in several structures of nervous (axon terminals, neuronal bodies, dendrites) and non-nervous nature (glial processes, capillaries). This fact led us to suggest that the chondroitin localizations advocate for their different functions. In their various localization exist distinct proteoglycans constituted by chondroitins with diverse concentrations and other attached radicals.
Gragera
R R
RR
Department of Cellular Biology, Faculty of Biology, Ciudad Universitaria s/n. Complutense University of Madrid, Spain.
Muñiz
E
E
De Esteban
G
G
Alonso
M J
MJ
López-Bravo
A
A
González-Romero
F J
FJ
Martínez-Murillo
R
R
Toledano
A
A
Martínez-Rodríguez
R
R
D000818:Animals; D002807:Chondroitin / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000737:chemistry / Q000648:ultrastructure*; D009474:Neurons / Q000737:chemistry; D009847:Olivary Nucleus / Q000648:ultrastructure*; D011509:Proteoglycans / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D006025:Glycosaminoglycans; D011509:Proteoglycans; D002807:Chondroitin