Structural and serological studies of the O-specific polysaccharide of the bacterium Proteus mirabilis O10 containing L-altruronic acid, a new component of O-antigens.
An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral L-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.
St Swierzko
A
A
Center of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland.
Shashkov
A S
AS
Senchenkova
S N
SN
Toukach
F V
FV
Ziolkowski
A
A
Cedzynski
M
M
Paramonov
N A
NA
Kaca
W
W
Knirel
Y A
YA
D000907:Antibodies, Bacterial / Q000097:blood; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006031:Glycosylation; D006461:Hemolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D010084:Oxidation-Reduction; D011233:Precipitin Tests; D011513:Proteus mirabilis / Q000276:immunology*; D014574:Uronic Acids / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000939:Epitopes; D019081:O Antigens; D014574:Uronic Acids; C102528:altruronic acid