title: [Structural and functional analysis of the human immunodeficiency glycoprotein gp120 with homologous synthetic peptides].
abstract: The purpose of the study was to identify sites of gp 120, which are responsible for CD4 binding and induce tumor necrosis factor-alpha (TNF-alpha) synthesis. Seven peptides were synthesized from gp 120. The peptides were studied in the following biological tests: binding to CD4 molecules of the Jurkat cell clones 3G6 and PBMC of healthy persons. There was TNF-alpha induction in healthy and HIV-positive individuals and its correlation was found with p24 antigenemia in HIV patients. The peptides 420-440, 426-452, 369-384, 255-272 bind to T-lymphocytic CD4 and induced TNF-alpha. The peptide 436-451 binds to CD4, but failed to induced TNF-alpha, which suggests that the latter may be used as a basis for HIV-infection vaccine.
authors.forename: S A
authors.initials: SA
authors.lastname: Ketlinskiĭ
authors.forename: N M
authors.initials: NM
authors.lastname: Kalinina
authors.forename: A N
authors.initials: AN
authors.lastname: Prusakov
authors.forename: O A
authors.initials: OA
authors.lastname: Kaurov
mesh_terms: D015704:CD4 Antigens / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000502:physiology*; D015658:HIV Infections / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D011485:Protein Binding; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D014409:Tumor Necrosis Factor-alpha
vernacular_title: Strukturno-funktsional'nyĭ analiz glikoproteina gp120 virusa immunodefitsita cheloveka s pomoshch'iu sinteticheskikh peptidov, gomologichnykh ego strukture.