Accelerated platelet reconstitution following transplantation of bone marrow cells derived from IL-6-treated donor mice.
We transplanted bone marrow cells derived from normal donor mice treated with IL-6 to study the effect on the hematopoietic recovery of lethally irradiated (8.5 Gy) recipients. Male Balb/C mice were treated for 7 days by continuous infusion of IL-6 (10 micrograms/day). Not only did these donor mice have increased numbers of circulating platelets as was previously shown; the numbers of circulating progenitor cells also increased more than 25-fold. Transplantation of nucleated bone marrow cells derived from these donor mice into lethally irradiated female recipients resulted in increased platelet nadir counts in comparison to recipients of normal bone marrow cells and similar to nadir counts of recipients of normal donor bone marrow treated with IL-6 for 7 days after transplantation. Combination of transplantation of bone marrow derived from IL-6 treated donors with post-transplantation treatment of the recipients with IL-6 resulted in a further increase in nadir counts, although it did not cause a further acceleration of platelet reconstitution. We conclude that transplantation of bone marrow cells modified in vivo by IL-6 results in significantly accelerated reconstitution of platelets, to a degree similar to that observed following treatment with IL-6 after transplantation.
Laterveer
L
L
Department of Hematology, University Medical Center Leiden, The Netherlands.
Zijlmans
J M
JM
Liehl
E
E
Willemze
R
R
Fibbe
W E
WE
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D015850:Interleukin-6 / Q000494:pharmacology*; D008297:Male; D008533:Megakaryocytes; D051379:Mice; D008807:Mice, Inbred BALB C; D010976:Platelet Count / Q000187:drug effects; D014916:Whole-Body Irradiation
D015850:Interleukin-6