title: RNA phage Q beta coat protein as a carrier for foreign epitopes.
abstract: The Q beta gene C has been proposed as a new carrier for the exposure of foreign peptide sequences. Contrary to well-known 'display vectors' on the basis of coat proteins of RNA phage group I, group III phage Q beta-based vectors suggested application of the 195-amino acid extension of coat protein (CP) within the so-called A1 protein for insertion of the appropriate immunological epitopes. 'Mosaic' capsids presenting model hepatitis B virus preS1 and HIV-1 gp120 epitopes and formed by Q beta CP together with A1-derived proteins were obtained as a result of (1) suppression of leaky UGA stop codon of the CP gene and (2) simultaneous expression of 'pure' CP and full-length A1-derived genes obtained after the changing of CP-terminating UGA to strong UAA stop codon or sense GGA codon, respectively.
authors.affiliation: Biomedical Research and Study Centre, University of Latvia, Riga, Latvia.
authors.forename: T M
authors.initials: TM
authors.lastname: Kozlovska
authors.forename: I
authors.initials: I
authors.lastname: Cielens
authors.forename: I
authors.initials: I
authors.lastname: Vasiljeva
authors.forename: A
authors.initials: A
authors.lastname: Strelnikova
authors.forename: A
authors.initials: A
authors.lastname: Kazaks
authors.forename: A
authors.initials: A
authors.lastname: Dislers
authors.forename: D
authors.initials: D
authors.lastname: Dreilina
authors.forename: V
authors.initials: V
authors.lastname: Ose
authors.forename: I
authors.initials: I
authors.lastname: Gusars
authors.forename: P
authors.initials: P
authors.lastname: Pumpens
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics*; D018388:Codon, Terminator; D000939:Epitopes / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010454:Peptide Termination Factors; D011498:Protein Precursors / Q000235:genetics; D012317:RNA Phages / Q000235:genetics*
chemical_list: D018388:Codon, Terminator; D000939:Epitopes; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D006514:Hepatitis B Surface Antigens; D010446:Peptide Fragments; D010454:Peptide Termination Factors; D011498:Protein Precursors; C087971:peptide-chain-release factor 3; C050806:presurface protein 1, hepatitis B surface antigen