Molecular characterisation of the Xenopus laevis chaperonin gene Cctg.
By library screening and PCR we have obtained cDNA clones which encode the gamma subunit of the CCT chaperonin complex from Xenopus laevis. The gene (XlCctg), which encodes the CCT gamma subunit contains an open reading frame which codes for 547 amino acid residues (60 kDa) and the predicted amino acid sequence shares a high degree of sequence identity with other CCT gamma homologues. The XlCctg mRNA measures 2.1 kb and is expressed ubiquitously in all of the X. laevis tissues examined. The mRNA levels of XlCctg are significantly higher in the ovary compared with other tissues.
Walkley
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School of Molecular and Medical Biosciences, University of Wales College of Cardiff, UK.
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Malik
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins