title: Sex-dependent expression of placental (P)-cadherin during mouse gonadogenesis.
abstract: BACKGROUND
  Placental (P)-cadherin is one of a family of cell adhesion molecules that participate in embryonic sorting and organogenesis. In previous work, P-cadherin was localized to Sertoli cells in the mouse testis as early as postnatal day 1. This early postnatal localization raised questions about when P-cadherin first appeared in the embryonic testis and whether P-cadherin was expressed differentially in the embryonic testis and ovary.
  
  
  METHODS
  The localization of P-cadherin, epithelial (E)-cadherin, and Müllerian inhibiting substance was determined in frozen sections of mouse gonads between embryonic days 10.5 and 18 using indirect immunohistochemistry. Alkaline phosphatase reactivity was used to identify germ cells.
  
  
  RESULTS
  The expression of P-cadherin was traced back to the indifferent stage of gonadogenesis where uniform distribution was observed in the indifferent gonad of both sexes. However, after sexual differentiation, the expression of P-cadherin in the testis was localized to Sertoli cells in the testicular cords, while its expression in the ovary fell below detectable levels.
  
  
  CONCLUSIONS
  The localization of P-cadherin in the male and female indifferent gonad is similar and cannot be used to distinguish the future testis and ovary. The localization of P-cadherin in the testis after sexual differentiation suggests a role for P-cadherin in testicular cord formation. The common temporal pattern of P-cadherin and Müllerian inhibiting substance expression in Sertoli cells is consistent with a shared regulatory mechanism.
authors.affiliation: Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University College of Medicine, Columbus 43210, USA.
authors.forename: L H
authors.initials: LH
authors.lastname: Lin
authors.forename: R M
authors.initials: RM
authors.lastname: DePhilip
mesh_terms: D000375:Aging; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D054304:Anti-Mullerian Hormone; D015820:Cadherins / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins; D006131:Growth Inhibitors / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010053:Ovary / Q000196:embryology* / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism; D012733:Sex Differentiation; D013735:Testicular Hormones / Q000378:metabolism; D013737:Testis / Q000196:embryology* / Q000378:metabolism*
chemical_list: D015820:Cadherins; D006023:Glycoproteins; D006131:Growth Inhibitors; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone; D000469:Alkaline Phosphatase