Suppression of T lymphocyte proliferation by peritoneal macrophages in chickens.
The mechanism of the suppression of lymphocyte proliferation by peritoneal macrophages (MPs) in chickens and the presence of a suppressive substance in the culture supernatant of MPs were studied. The results showed that gelatin- and peptone-induced peritoneal MPs had an inhibitory accessory function against blastogenesis of mitogen-stimulated splenic lymphocytes and that resident peritoneal MPs had an enhanced accessory function. The suppressive factor produced by gelatin-induced peritoneal MPs is a protein having a pI of 3.6 and a molecular weight of 64,000 and it is physically and chemically a relatively stable substance. Moreover, it has no cytotoxic effect against lymphocytes and it was verified that the suppressor in the culture supernatant of MPs was not prostaglandin E2 or alpha-acid glycoprotein.
Date
K
K
Department of Veterinary Immunology, College of Agriculture, University of Osaka Prefecture, Japan.
Okamoto
T
T
Kurata
M
M
Babe
T
T
Kodama
H
H
Mukamoto
M
M
Tsuji
S
S
D000818:Animals; D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D002645:Chickens; D003602:Cytotoxicity, Immunologic; D005780:Gelatin / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D011506:Proteins; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D007166:Immunosuppressive Agents; D011506:Proteins; C046429:macrophage-derived immunosuppressor factor; D005780:Gelatin