title: Titration of human cytomegalovirus (HCMV) DNA in urine by combined use of PCR and microplate hybridization in a renal transplant patient with HCMV pneumonitis.
abstract: We titrated human cytomegalovirus (HCMV) DNA in urine specimens obtained from 14 healthy individuals and a renal transplant patient with HCMV pneumonitis by modifying the method for titration of varicella-zoster virus DNA previously described (1,2). Of 14 HCMV seropositive healthy individuals, 13 had HCMV DNA under the detection limit of 10(2.0) copies/ml, whereas one person had 10(2.0) copies/ml. The viral DNA in urine samples was at a low level in healthy individuals with latent infection. In a case with HCMV pneumonitis after renal transplantation, the amount of HCMV DNA in urine gradually increased from the level under 10(2.0) copies/ml and reached a peak of 10(4.7) copies/ml one month prior to the manifestation of pneumonitis. It, thereafter, decreased with the course of clinical remission, and finally settled at under 10(2.0) copies/ml. Serial titrations of HCMV DNA in urine specimens proved to be useful in identifying recipients at risk of developing active HCMV infection after renal transplantation and as a guide for treatment of patients.
authors.affiliation: Department of Surgery and Organ Transplantation, University of Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Meigata
authors.forename: R
authors.initials: R
authors.lastname: Hondo
authors.forename: A
authors.initials: A
authors.lastname: Fujima
authors.forename: M
authors.initials: M
authors.lastname: Shinkai-Shibata
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
authors.forename: N
authors.initials: N
authors.lastname: Ichikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Degawa
authors.forename: Y
authors.initials: Y
authors.lastname: Beck
authors.forename: S
authors.initials: S
authors.lastname: Tomikawa
authors.forename: T
authors.initials: T
authors.lastname: Nagao
authors.forename: H
authors.initials: H
authors.lastname: Uchida
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000652:urine*; D006801:Humans; D017403:In Situ Hybridization; D007564:Japan / Q000453:epidemiology; D016030:Kidney Transplantation; D011014:Pneumonia / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral