Widespread increase in brain protein synthesis following acute immobilization stress in adult rat brain.
The autoradiographic method with [L-35S]methionine was used to determine the effects of a 2 h acute immobilization stress followed by a 4 h recovery on local rates of protein synthesis in the adult rat brain. Methionine incorporation into proteins was significantly increased (from 17 to 86%) in 37 out of the 39 analyzed brain structures. These results show that the stress-induced activation of the overall rate of brain protein synthesis may persist for at least 4 h after cessation of the stimulus even though the stress-related physiological variables have returned to basal levels. They suggest that increased protein synthesis may play a key role in the molecular events which lead to the neuronal plastic changes following an acute stress.
Baubet
V
V
CNRS ERS 5645, INSERM U52, Laboratoire de Médecine Expérimentale, UFR Rockefeller, Lyon, France.
Grange
E
E
Sermet
E
E
Giaume
M
M
Gay
N
N
Bobillier
P
P
D000208:Acute Disease; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D007103:Immobilization; D008297:Male; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism*; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins; D008715:Methionine