title: Heat-stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide.
abstract: The role of lipopolysaccharide (LPS) in the serotyping of Campylobacter jejuni based on heat-stable antigens was examined using SDS-PAGE and a silver stain for carbohydrate. None of the 32 type strains of Camp. jejuni expressed long-chain LPS. Rabbit antibodies, prepared to 10 selected strains of Camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not LPS. This study suggests that the heat-stable antigens of Camp. jejuni, which form the basis for the established Penner serotyping scheme, are probably capsular and not LPS.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Chart
authors.forename: J A
authors.initials: JA
authors.lastname: Frost
authors.forename: A
authors.initials: A
authors.lastname: Oza
authors.forename: R
authors.initials: R
authors.lastname: Thwaites
authors.forename: S
authors.initials: S
authors.lastname: Gillanders
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000145:classification* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000276:immunology; D006388:Hemagglutinins; D006358:Hot Temperature; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D006388:Hemagglutinins; D008070:Lipopolysaccharides