Cloning and characterization of two novel human cDNAs (NELL1 and NELL2) encoding proteins with six EGF-like repeats.
From a human fetal-brain cDNA library we isolated two novel genes encoding peptides containing six EGF-like repeats. Both showed significant homologies with nel, a gene strongly expressed in neural tissues of chicken. The cDNAs, designated NELL1 (nel-like, type 1) and NELL2 (nel-like, type 2), contained open reading frames encoding 810 and 816 amino acids, respectively. NELL2 is strongly expressed in brain of adult and fetus but only weakly in fetal kidney. NELL1 and NELL2 were mapped by FISH to chromosomal bands 11p15.1-p15.2 and 12q13.11-q13.12, respectively.
Watanabe
T K
TK
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima, 771-01, Japan.
Katagiri
T
T
Suzuki
M
M
Shimizu
F
F
Fujiwara
T
T
Kanemoto
N
N
Nakamura
Y
Y
Hirai
Y
Y
Maekawa
H
H
Takahashi
E i
Ei
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002135:Calcium-Binding Proteins; D002645:Chickens / Q000235:genetics; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000737:chemistry*; D005326:Fetal Proteins / Q000235:genetics; D005333:Fetus / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D005326:Fetal Proteins; C104144:NELL1 protein, human; C104145:NELL2 protein, human; D009419:Nerve Tissue Proteins; D004815:Epidermal Growth Factor