title: Differential effects of ketamine and pentobarbitone on acetylcholine release from the rat hippocampus and striatum.
abstract: Using microdialysis, we examined the effects of ketamine and pentobarbitone on acetylcholine (ACh) release from the rat hippocampus and striatum. Ketamine 25 and 50 mg kg-1 increased ACh release from the hippocampus to 295% and 353% of basal release, respectively, but not from the striatum. SCH 23390 1 mumol litre-1, a D1 antagonist, significantly inhibited the facilitatory effect of ketamine 50 mg kg-1 on hippocampal ACh release (to 241% of basal level). In contrast, pentobarbitone 20 and 40 mumg kg-1 decreased basal ACh release from both the hippocampus by 41% and 69%, respectively, and the striatum by 37% and 58%, respectively. The results suggest that ketamine and pentobarbitone exert opposite effects on ACh release from the rat hippocampus and that the stimulating effect of ketamine may involve dopamine D1 receptors.
authors.affiliation: Department of Anesthesiology, Yokohama City University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: I
authors.initials: I
authors.lastname: Watanabe
authors.forename: F
authors.initials: F
authors.lastname: Okumura
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000778:Anesthetics, Dissociative / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology*; D008297:Male; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000778:Anesthetics, Dissociative; D006993:Hypnotics and Sedatives; D007649:Ketamine; D010424:Pentobarbital; D000109:Acetylcholine