Characterization of a human gene related to genes encoding somatostatin receptors.
We report the identification of a gene, named SLC-1(1), encoding a novel G protein-coupled receptor (GPCR). A customized search procedure of a database of expressed sequence tags (dbEST) retrieved a human cDNA sequence that partially encoded a GPCR. A genomic DNA fragment identical to the cDNA was obtained and used to screen a library to isolate the full-length coding region of the gene. This gene was intronless in its open reading frame, and encoded a receptor of 402 amino acids, and shared -40% amino acid identity in the transmembrane (TM) regions to the five known human somatostatin receptors. Northern blot analysis revealed that SLC-1 is expressed in human brain regions, including the forebrain and hypothalamus. Expression in the rat was highest in brain, followed by heart, kidney, and ovary. Expression of SLC-1 in COS-7 cells failed to show specific binding to radiolabelled Tyr1-somatostatin-14, naloxone, bremazocine, 1,3-di(2-tolyl)-guanidine (DTG), or haloperidol. A repeat polymorphism of the form (CA)n was discovered in the 5'-untranslated region (UTR) of the gene and SLC-1 was mapped to chromosome 22, q13.3.
Kolakowski
L F
LF
Department of Pharmacology, University of Texas Health Science Center at San Antonio, 78284, USA.
Jung
B P
BP
Nguyen
T
T
Johnson
M P
MP
Lynch
K R
KR
Cheng
R
R
Heng
H H
HH
George
S R
SR
O'Dowd
B F
BF
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018900:Dinucleotide Repeats; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008024:Ligands; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017481:Receptors, Somatostatin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013004:Somatostatin / Q000031:analogs & derivatives / Q000378:metabolism
D018076:DNA, Complementary; D008024:Ligands; C103263:MCHR1 protein, human; C491208:MCHR1 protein, rat; D017481:Receptors, Somatostatin; D013004:Somatostatin; C012088:somatostatin, N-Tyr(1)-; D019204:GTP-Binding Proteins