Capillary electrophoresis of closely related intrinsic thylakoid membrane proteins of the photosystem II light-harvesting complex (LHC II).
The electrophoretic migration behavior of three closely related hydrophobic intrinsic membrane proteins of the photosystem II light-harvesting complex (LHC II) was investigated in free solution capillary electrophoresis at pH 8.0-10 with running electrolyte solutions containing either anionic, zwitter-ionic or nonionic detergents. The complete and repeatable separation of these proteins was accomplished with a running electrolyte solution of 25 mM Tris/192 mM glycine, pH 8.8, containing either sodium dodecyl sulfate or n-octyl beta-D-glucopyranoside at concentration up to 5.0 and 7.0 mM, respectively. Migration times and resolution of the individual LHC II intrinsic membrane proteins were sensitive to the type of detergent. The effect of detergent concentration on the electrophoretic behavior of the LHC II proteins was also investigated. Electroelution of the LHC II components separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to isolate these intrinsic membrane proteins, which were then injected onto the capillary electrophoresis system for peak identification.
Zolla
L
L
Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, Italy. zolla@unitus.it
Bianchetti
M
M
Timperio
A M
AM
Mugnozza
G S
GS
Corradini
D
D
D002736:Chloroplasts / Q000648:ultrastructure*; D003902:Detergents; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae; D005960:Glucosides; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis*; D045332:Photosystem II Protein Complex; D010946:Plants, Medicinal; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D018724:Spinacia oleracea
D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D012996:Solutions; C018619:octyl-beta-D-glucoside; D012967:Sodium Dodecyl Sulfate