Antimicrobial and immunological activity of ethanol extracts and fractions from Isopyrum thalictroides.
The antimicrobial and immunological properties of ethanol extracts, non-alkaloid, tertiary alkaloid and quaternary alkaloid fractions, obtained from roots and aerial parts of Isopyrum thalictroides were examined. The non-alkaloid fraction from aerial parts inhibited the growth of seven test microorganisms and was the most effective suppressor of classical pathway (CP) complement activity in normal human serum (NHS) and guinea pig serum (GPS). The alkaloid fractions, containing quaternary alkaloids expressed suppressive effect on mitogen-induced splenocyte proliferation. The in vitro antibody response against sheep red blood cells (anti-SRBC) was inhibited by ethanol extracts and quaternary alkaloid fraction. The intraperitoneal (i.p.) application of ethanol extract and tertiary alkaloid fraction from aerial parts showed that they possess in vivo effect on alternative pathway (AP) complement activity, anti-SRBC response and delayed type hypersensitivity (DTH).
Ivanovska
N
N
Department of Immunology, Bulgarian Academy of Sciences, Sofia.
Philipov
S
S
Istatkova
R
R
Georgieva
P
P
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D000431:Ethanol / Q000737:chemistry; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012756:Sheep
D000276:Adjuvants, Immunologic; D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D000431:Ethanol