title: Chromosomal localization of the mouse and rat DNA double-strand break repair genes Ku p70 and Ku p80/XRCC5 and their mRNA expression in various mouse tissues.
abstract: The Ku p70 and Ku p80/XRCC5 genes are involved in DNA double-strand break repair and V(D)J recombination, and their gene products are the components of the DNA-dependent protein kinase. We have determined the chromosomal locations of the mouse Ku p70 and Ku p80/XRCC5 genes by both in situ hybridization and molecular linkage analysis: the Ku p70 gene was localized to mouse chromosome 15 and rat chromosome 7, and the Ku p80/XRCC5 gene was localized to mouse chromosome 1 and rat chromosome 9. Both genes were mapped to a region of conserved linkage homology among three species, i.e., the mouse, rat, and human. Molecular linkage analysis using interspecific backcross mice revealed that the murine Ku p70 locus was localized 0.7 cM terminal to D15Mit1 and that the murine Ku p80/XRCC5 locus was 0.7 cM proximal to D1Mit46. To determine the size and tissue transcription specificity of the mouse Ku p70 and Ku p80/XRCC5 mRNA, Northern blot analysis was carried out with six mouse tissues. Each tissue expressed one species of the Ku p70 gene transcript with 2.4 kb and one species of the Ku p80/XRCC5 gene transcript with 2.6 kb. In the latter case, however, the brain showed two sizes of transcript, 2.6 and 2.9 kb.
authors.affiliation: Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Koike
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Mimori
authors.forename: Y N
authors.initials: YN
authors.lastname: Harada
authors.forename: N
authors.initials: N
authors.lastname: Shiomi
authors.forename: T
authors.initials: T
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear; D002874:Chromosome Mapping; D004265:DNA Helicases; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D000072200:Ku Autoantigen; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606512:Xrcc5 protein, mouse; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; C000606511:Xrcc6 protein, rat; D000072200:Ku Autoantigen