Capture in the gel: intermoleculare triplex formation during gel electrophoresis.
Analysis of unusual gel mobility patterns formed by certain DNA triplexes has revealed that intermolecular triplex formation can occur during gel electrophoresis when a faster migrating single strand overtakes a slower migrating band containing a duplex of appropriate sequence. Control experiments showed that this capture of the third strand occurs by sequence-specific hybridization rather than some nonspecific retardation. This phenomenon can be used to detect triplexes by a gel-shift assay even if their lifetime is much shorter than the time of gel electrophoresis.
Belotserkovskii
B P
BP
Cell and Molecular Biology Laboratory, SRI International, Menlo Park, CA 94025, USA.
Johnston
B H
BH
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry
D009841:Oligonucleotides; D004247:DNA