Oct-6 (SCIP/Tst-1) is expressed in Schwann cell precursors, embryonic Schwann cells, and postnatal myelinating Schwann cells: comparison with Oct-1, Krox-20, and Pax-3.
The POU domain transcription factor Oct-6 (SCIP/Tst-1) is likely to control important stages of Schwann cell development, including the initiation of myelination around birth. Here, we use immunocytochemical and reverse transcriptase-polymerase chain reaction techniques to examine Oct-6 earlier in nerve development, to test the idea that Oct-6 has an additional role in Schwann cell precursors or early embryonic Schwann cells, a possibility raised by previous studies on transgenic mice. Consistent with this, we find low but unambiguous levels of Oct-6 mRNA and protein in Schwann cell precursors of mouse and rat (nerves from 12- and 14-day-old embryos, respectively), with expression levels gradually increasing during early Schwann cell development and towards birth. Unexpectedly, Oct-6 immunoreactivity is clearly present in nuclei of most myelinating cells at least as late as postnatal day 12. Furthermore, many nonmyelinating Schwann cells express Oct-6 in adult life. A comparison of Oct-6 mRNA with other Schwann cell transcription factors-namely, Oct-1, Krox-20, and Pax-3-reveals that each factor exhibits strong developmental regulation and a unique expression pattern in embryonic nerves. Therefore, they are likely to play distinct regulatory roles in early development of the Schwann cell lineage.
Blanchard
A D
AD
Department of Anatomy and Developmental Biology, University College London, England.
Sinanan
A
A
Parmantier
E
E
Zwart
R
R
Broos
L
L
Meijer
D
D
Meier
C
C
Jessen
K R
KR
Mirsky
R
R
D000818:Animals; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D051767:Early Growth Response Protein 2; D005326:Fetal Proteins / Q000096:biosynthesis / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D051863:Host Cell Factor C1; D017403:In Situ Hybridization; D051379:Mice; D009186:Myelin Sheath / Q000502:physiology*; D009413:Nerve Fibers, Myelinated / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D050811:Octamer Transcription Factor-1; D050816:Octamer Transcription Factor-6; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D010525:Peripheral Nerves / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; C497328:Egr2 protein, rat; D005326:Fetal Proteins; C497693:Hcfc1 protein, mouse; D018398:Homeodomain Proteins; D051863:Host Cell Factor C1; D009419:Nerve Tissue Proteins; D050811:Octamer Transcription Factor-1; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; C494166:Pou2f1 protein, mouse; C494168:Pou2f1 protein, rat; C494180:Pou3f1 protein, mouse; C494179:Pou3f1 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; C068686:Pax3 protein, mouse