[Mechanism of retaining stability of foot-and-mouth disease virus during manufacturing dried concentrated preparations].
Removal of moisture from concentrated virus suspensions in a sequential manner using ultrafiltration, active ventilation and vacuum dehydration of concentrated powders enables us to obtain preparations with a residual content of moisture less than 1%, while the structure and properties of FMD virus are retained. Mechanism of inactivation of FMD virus in drying is attributed to electrophysical processes associated with development of electric potentials in evaporated objects due to directional movement of charged water molecules. Having reached certain values, outer electric potential induces charge exchange in virions that leads to the break of energy balance in the structure of virus particles and to their disruption when rehydrating in buffer solution.
Ponomarev
A P
AP
Andreeva
O G
OG
Uziumov
V L
VL
D005537:Aphthovirus / Q000302:isolation & purification*; D005612:Freeze Drying
Mekhanizm sokhraneniia ustoĭchivosti virusa iashchura pri poluchenii sukhikh kontsentrirovannykh preparatov.