title: Detection of antioxidant enzyme activities in renal tissues of early stage IgA nephropathy in ddY mice.
abstract: The purpose of this study was to determine the antioxidant enzyme activities in renal tissues of early stage ddY mice, an animal model for primary IgA nephropathy. Eight- and 40-week-old ddY female mice and normal healthy Balb/c female mice were used in this study. The levels of Cu/Zn-SOD, Mn-SOD, and GSH-PX activities in the renal cortex were significantly higher in 40-week-old ddY mice than in Balb/c control mice of the same age; no change of catalase activity was observed. There were no significant differences in the levels of Cu/Zn-SOD, Mn-SOD, GSH-PX, and catalase activities between the ddY mice and Balb/c mice at 8 weeks of age. Urinary protein was slightly higher in 40-week-old ddY mice. IgA or C3 was deposited at low levels in the glomerular mesangial areas of 8-week-old ddY mice. Marked depositions of IgA and C3 extended from the glomerular mesangial areas to the capillary walls of 40-week-old ddY mice. Expansion of glomerular mesangial matrices and mild mesangial cell proliferation was observed in 40-week-old ddY mice. Antioxidant enzyme activities in the renal cortex were already increased in the early stage IgA nephropathy in 40-week-old ddY mice. These findings suggest that measurements of antioxidant enzyme activities in the renal cortex of 40-week-old ddY mice was useful for evaluation of the pathogenesis of renal involvement in the early stage of IgA nephropathy.
authors.affiliation: Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: L N
authors.initials: LN
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Fukui
authors.forename: I
authors.initials: I
authors.lastname: Shou
authors.forename: Y
authors.initials: Y
authors.lastname: Yaguchi
authors.forename: K
authors.initials: K
authors.lastname: Funabiki
authors.forename: S
authors.initials: S
authors.lastname: Horikoshi
authors.forename: I
authors.initials: I
authors.lastname: Shirato
authors.forename: Y
authors.initials: Y
authors.lastname: Tomino
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D003176:Complement C3 / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000737:chemistry / Q000378:metabolism; D005922:Glomerulonephritis, IGA / Q000378:metabolism*; D005979:Glutathione Peroxidase; D007070:Immunoglobulin A / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000293:injuries / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D011507:Proteinuria; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003176:Complement C3; D007070:Immunoglobulin A; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase