Differential production of interleukin-6 and its close relation to liver metastasis in clones from murine P815 mastocytoma.
Interleukin-6 (IL-6)-producing abilities of plastic-adherent and plastic-non-adherent P815 clones were investigated in connection with the liver metastasis. Most adherent clones produced IL-6 at high level (over 10 ng/10(5) cells per 48 h), and they coincided with highly liver-metastatic clones. The remaining adherent and all the non-adherent clones tested produced IL-6 at low level (under 1.5 ng/10(5) cells), and they coincided with the low or non-liver-metastatic clones. The IL-6 production was greatly enhanced by IL-1 alpha, but not by IL-6, tumor necrosis factor-alpha or interferon-gamma.
Hojo
H
H
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Sun
R
R
Ono
Y
Y
Shishido
T
T
Obara
E
E
Yamazoe
Y
Y
Hashimoto
Y
Y
D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D002999:Clone Cells / Q000187:drug effects / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D012515:Mast-Cell Sarcoma / Q000378:metabolism / Q000556:secondary*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008811:Mice, Inbred DBA; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D015850:Interleukin-6; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma