B lymphocytes are impaired in mice lacking the transcriptional co-activator Bob1/OCA-B/OBF1.
The protein Bob1 (also called OCA-B or OBF-1) is a B lymphocyte-specific transcriptional co-activator which can stimulate transcription by interaction with Oct-1 or Oct-2 and the octamer sequence found in the promoter and most enhancer regions of the immunoglobulin genes. The role that Bob1 plays in the maturation and function of B cells was investigated in mice deficient for Bob1. Although early stages of B cell development in the bone marrow do not appear to be affected, these mice show reduced numbers of B cells in peripheral lymphoid organs. Based on staining for B220, heat-stable antigen, and IgD, this reduction is particularly strong in those cells representing the more mature B cell stages. Probably as a consequence of this reduction in mature B cells, Bob1-deficient mice show reduced serum titers of the immunoglobulin isotypes IgG1, IgG2a, IgG2b and IgA, but not IgM. Histological examination of sections from spleen and lymph nodes reveal that while Bob-1-deficient mice have primary follicles, they lack well-developed germinal centers. Interestingly, B1 (Ly1) B cells in the peritoneum do not appear to be affected by the lack of Bob1. Taken together, these results suggest that, at least in conventional B cells, Bob1 plays in important role in the antigen-driven stages of B cell activation and maturation.
Nielsen
P J
PJ
Max Planck Institute for Immunobiology, Freiburg, Germany. nielsen@immunbio.mpg.de
Georgiev
O
O
Lorenz
B
B
Schaffner
W
W
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000276:immunology*
C490424:Pou2af1 protein, mouse; D015534:Trans-Activators