Dried sera for confirming blood-borne virus infections (HCV, HTLV-I, HIV & HBsAg).
For safe blood transfusion, developing countries face considerable problems including serological screening and confirmation of blood-borne virus infections (HCV, HTLV-I, HIV and HBsAg). Confirmation tests are not only costly but also require sophisticated techniques and expertise. In order to provide this support we have attempted to perform a virus antibody confirmation test on samples dried on blotting paper (BP). Forty-nine sera derived from selected patients and donors from Bombay, and nine donors' sera from Bellarussia were transported on BP. In control experiments, dilutions of antibody-positive sera (HIV, HTLV-I & HCV) and 'blinded' HTLV-I antibody-positive and antibody-negative donors were applied on BP. Eluates from snipped BP were tested initially by screening tests, and the reactives were subjected to confirmatory tests for three types of virus antibody tests (HCV, HTLV-I & HIV) by blotting methods and neutralisation tests for HBsAg. There was considerable reduction of titres in dry sera but all BP-derived dry specimens gave excellent qualitative concordance with their liquid-equivalent sera, and the HTLV-I-positive donor was identified and reconfirmed correctly. Presence of only HCV antibody was confirmed in all the nine selected Bellarussian donors. Blood donors in Bombay had 3% HIV antibody, 6% HBsAg and none had HCV antibody, while selected patients showed substantially higher levels of these markers: HIV-antibody 64%, HBsAg 57% and HCV-antibody 17% confirmed positive. The cause of this high level remains to be established. Dry samples received by post seem to be an economical approach to a first step in providing some levels of independent confirmation of reactives in developing countries.
Das
P C
PC
Red Cross Blood Bank Noord Nederland, Groningen, The Netherlands.
de Vries
A H
AH
McShine
R L
RL
Sibinga
C T
CT
D000914:Antibodies, Viral / Q000097:blood*; D005612:Freeze Drying; D015497:HIV-1 / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D065227:Transfusion Reaction; D014777:Virus Diseases / Q000517:prevention & control*
D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens