Isolation of a carbon source-regulated gene from Ustilago maydis.
We have isolated a carbon source-regulated gene from the phytopathogenic fungus Ustilago maydis by use of a promoter-probe vector. This gene, called crg1, is strongly induced by L-arabinose and efficiently repressed by D-glucose and D-xylose. The predicted 36.5-kDa mature crg1 gene product lacks similarity to known proteins but is likely to be secreted. Sequences required for regulated expression of a reporter gene are contained within a 3.6-kb fragment upstream of the crg1 gene. The promoter of crg1 fulfils requirements for an efficient controllable gene expression system in U. maydis
Bottin
A
A
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Kämper
J
J
Kahmann
R
R
D000595:Amino Acid Sequence; D001089:Arabinose / Q000378:metabolism; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004926:Escherichia coli; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014588:Ustilago / Q000235:genetics*; D014994:Xylose / Q000378:metabolism
C103756:CRG1 protein, Ustilago maydis; D004271:DNA, Fungal; D005656:Fungal Proteins; D002244:Carbon; D014994:Xylose; D001089:Arabinose; D005947:Glucose