title: Intercellular adhesion molecule-1 expression on glia following brain injury: participation of interleukin-1 beta.
abstract: Interleukin-1 (IL-1), one of the most important inflammatory cytokines, promotes glia to express intercellular adhesion molecule-1 (ICAM-1) in vitro. IL-1 is known to be produced in situ immediately after brain insults and recently we have found that glia, including astrocytes, express ICAM-1 in vivo following cortical stab wounds. To evaluate the participation of IL-1 beta in posttraumatic ICAM-1 expression on glia in vivo, we performed the following experiments. A cortical stab wound was made in the brain of a mouse. ICAM-1-immunopositive glia began to emerge around the wound from 6 h postlesioning. The number of cells reached a maximum at 48 h and persisted until 7 days postlesioning. Next, a neutralizing monoclonal antibody against IL-1 beta was infused into the wound immediately following the injury. This treatment resulted in a significant reduction of ICAM-1-positive glia at 24 and 48 h postlesioning. We conclude, therefore, that IL-1 beta affects ICAM-1 expression on glia in vivo after experimental brain injury and presumably plays an important role in brain wound repair.
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shibayama
authors.forename: H
authors.initials: H
authors.lastname: Kuchiwaki
authors.forename: S
authors.initials: S
authors.lastname: Inao
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Ito
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002452:Cell Count; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000906:Antibodies; D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1