p300 family members associate with the carboxyl terminus of simian virus 40 large tumor antigen.
Several cellular polypeptides critical for growth regulation interact with DNA tumor virus oncoproteins. p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, but it is structurally and immunologically closely related to p300 and CREB-binding protein, two known E1A-binding transcription adapters. Like p300, p400 is a phosphoprotein that binds to the simian virus 40 large tumor antigen (T). In anti-T coimmunoprecipitation experiments, staggered deletions spanning the amino-terminal 250 amino acids of T did not abrogate T binding to either p400 or p300. A T species composed of residues 251 to 708 bound both p400 and p300, while a T species defective in p53 binding was unable to bind either detectably. Anti-p53 immunoprecipitates prepared from cells containing wild-type T also contained p400 and p300. Hence, both p400 and p300 can bind (directly or indirectly) to a carboxyl-terminal fragment of T which contains its p53 binding domain. Since the p53 binding domain of T contributes to its immortalizing and transforming activities, T-p400 and/or T-p300 interactions may participate in these functions.
Lill
N L
NL
Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Tevethia
M J
MJ
Eckner
R
R
Livingston
D M
DM
Modjtahedi
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D001665:Binding Sites; D050882:CREB-Binding Protein; D002460:Cell Line; D050881:E1A-Associated p300 Protein; D051379:Mice; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats; D015534:Trans-Activators; D014157:Transcription Factors / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse; C494365:Crebbp protein, rat; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; C494362:Ep300 protein, rat