Effect of side groups on the action of beta-xylosidase from Trichoderma reesei against substituted xylo-oligosaccharides.
The action of beta-xylosidase from Trichoderma reesei against different substituted xylo-oligosaccharides was studied. The enzyme cleaved off all unsubstituted xylose units from the non-reducing end of 1,2-linked uronic acid substituted xylo-oligosaccharides. Surprisingly, an L-arabinofuranosyl group linked alpha-1,3 to the xylopyranosyl ring was found to protect the beta-1,4-xylosidic linkage before the substituted xylose unit from being cleaved by the beta-xylosidase. Most probably the 1,3-linked substituent sterically hinders the hydrolysis. According to the results of the present work, beta-xylosidase of T. reesei is not able to remove all unsubstituted xylose units from the non-reducing end of substituted xylo-oligosaccharides, as had been believed previously.
Tenkanen
M
M
VTT Biotechnology and Food Research, Finland. Maija.Tenkanen@vtt.fi
Luonteri
E
E
Teleman
A
A
D002852:Chromatography, Ion Exchange; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry / Q000378:metabolism*
D009844:Oligosaccharides; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase