title: On the molecular basis and regulation of cellular capacitative calcium entry: roles for Trp proteins.
abstract: During the last 2 years, our laboratory has worked on the elucidation of the molecular basis of capacitative calcium entry (CCE) into cells. Specifically, we tested the hypothesis that CCE channels are formed of subunits encoded in genes related to the Drosophila trp gene. The first step in this pursuit was to search for mammalian trp genes. We found not one but six mammalian genes and cloned several of their cDNAs, some in their full length. As assayed in mammalian cells, overexpression of some mammalian Trps increases CCE, while expression of partial trp cDNAs in antisense orientation can interfere with endogenous CCE. These findings provided a firm connection between CCE and mammalian Trps. This article reviews the known forms of CCE and highlights unanswered questions in our understanding of intracellular Ca2+ homeostasis and the physiological roles of CCE.
authors.affiliation: Department of Anesthesiology, School of Medicine, University of California, Los Angeles 90095, USA. lutz@cvmail.anes.ucla.edu
authors.forename: L
authors.initials: L
authors.lastname: Birnbaumer
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: G
authors.initials: G
authors.lastname: Boulay
authors.forename: M
authors.initials: M
authors.lastname: Peyton
authors.forename: B
authors.initials: B
authors.lastname: Vannier
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: D
authors.initials: D
authors.lastname: Platano
authors.forename: H
authors.initials: H
authors.lastname: Sadeghi
authors.forename: E
authors.initials: E
authors.lastname: Stefani
authors.forename: M
authors.initials: M
authors.lastname: Birnbaumer
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004330:Drosophila; D019204:GTP-Binding Proteins / Q000502:physiology*; D007448:Invertebrates; D008322:Mammals; D008954:Models, Biological; D008961:Models, Structural; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D050052:TRPC Cation Channels
chemical_list: D015220:Calcium Channels; D011994:Recombinant Proteins; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D019204:GTP-Binding Proteins; D002118:Calcium