title: Disease patterns and tissue cytokine profiles in giant cell arteritis.
abstract: OBJECTIVE
  To determine whether clinical heterogeneity in patients with giant cell arteritis (GCA) is correlated with different patterns in the tissue-specific inflammatory response.
  
  
  METHODS
  Twenty-three patients with typical histomorphologic findings of GCA were grouped according to the presence or absence of jaw claudication and/or visual abnormalities, fever, concomitant polymyalgia rheumatica (PMR), and histologic evidence of giant cell formation. The inflammatory response in temporal artery biopsy specimens was characterized by semiquantification of cytokine messenger RNA (mRNA) transcripts using reverse transcriptase-polymerase chain reaction, followed by oligonucleotide hybridization with cytokine-specific probes. Clinical patterns were then correlated with profiles of tissue cytokines.
  
  
  RESULTS
  Inflammatory cytokines were expressed in all temporal artery tissues. In situ synthesis of interleukin-2 (IL-2), interferon-gamma (IFN gamma), and IL-1 beta mRNA, but not of IL-10 and IL-12 mRNA, distinguished different patterns of inflammation, and these patterns correlated with clinical manifestations of the disease. Patients with evidence of ischemic symptoms, indicated by jaw claudication and/or visual symptoms, typically expressed higher concentrations of IFN gamma mRNA (P = 0.008) and IL-1 beta mRNA (P = 0.02). Presence of fever was correlated with lower copy numbers of IFN gamma (P = 0.02). Formation of giant cells in the granulomatous infiltrates was associated with the local synthesis of IFN gamma mRNA (P = 0.003). Tissue from GCA patients with concomitant PMR contained higher levels of IL-2 mRNA transcripts (P = 0.001).
  
  
  CONCLUSIONS
  Variations in the clinical presentation of GCA were correlated with cytokine mRNA expression in the affected temporal arteries. Differences in the effector functions of tissue-infiltrating T cells distinguished disease patterns in which either local ischemic symptoms or systemic involvement was dominant, or in which there was co-occurrence of PMR. Definition of different patterns of inflammation in GCA might, therefore, facilitate the design of differentiated therapeutic approaches.
authors.affiliation: Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Weyand
authors.forename: N
authors.initials: N
authors.lastname: Tetzlaff
authors.forename: J
authors.initials: J
authors.lastname: Björnsson
authors.forename: A
authors.initials: A
authors.lastname: Brack
authors.forename: B
authors.initials: B
authors.lastname: Younge
authors.forename: J J
authors.initials: JJ
authors.lastname: Goronzy
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D013700:Giant Cell Arteritis / Q000209:etiology* / Q000378:metabolism; D006801:Humans; D007383:Intermittent Claudication / Q000503:physiopathology; D007571:Jaw Diseases / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D013699:Temporal Arteries / Q000737:chemistry / Q000473:pathology; D014657:Vasculitis / Q000378:metabolism / Q000473:pathology; D014785:Vision, Ocular / Q000502:physiology
chemical_list: D016207:Cytokines; D012333:RNA, Messenger