title: Pooled human immunoglobulins contain anti-idiotypes with reactivity against the SLE-associated 4B4 cross-reactive idiotype.
abstract: OBJECTIVE
  To identify anti-idiotype antibody activity against a regulatory anti-Sm idiotype (4B4) in pooled human IgG (IVIG).
  
  
  METHODS
  ELISA were used to determine anti-idiotype antibody activity in IVIG.
  
  
  RESULTS
  Anti-idiotype antibody activity to 4B4 was detected in IVIG. The anti-idiotypic activity in IVIG could be inhibited by antigen, Sm, and rabbit anti-idiotypic antiserum. The idiotypic binding activity was predominately directed against the 4B4 VH region.
  
  
  CONCLUSIONS
  IVIG contains antibodies against a lupus-associated crossreactive idiotype. These antibodies represent Ab2 beta type antibodies. The anti-idiotypic activity in IVIG may be relevant to the efficacious use of IVIG in treating rheumatic diseases.
authors.affiliation: University of Texas Health Science Center, Department of Medicine, San Antonio, USA.
authors.forename: F
authors.initials: F
authors.lastname: DeKeyser
authors.forename: H
authors.initials: H
authors.lastname: DeKeyser
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
authors.forename: F
authors.initials: F
authors.lastname: Rossi
authors.forename: H
authors.initials: H
authors.lastname: Dang
authors.forename: N
authors.initials: N
authors.lastname: Talal
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011817:Rabbits
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D016756:Immunoglobulins, Intravenous