Direct determination of diuretic drugs in urine by capillary zone electrophoresis using fluorescence detection.
Four diuretic drugs banned in sport (amiloride, triamterene, bendroflumethiazide and bumetanide) have been separated by capillary zone electrophoresis (CZE) and detected using conventional fluorescence spectrometry. The effect of pH on electrophoretic parameters such as migration time, peak efficiency and peak height is discussed. Complete separation of the four drugs is achieved in less than 8 min at pH 8. No interference due to endogenous urine components is observed and thus direct urine analysis is feasible. Analytical figures of merit including precision and limits of detection are presented. Limits of detection range between 0.5 fmol for triamterene and 21.6 fmol for bumetanide.
González
E
E
Department of Analytical Chemistry, University of Málaga, Spain.
Becerra
A
A
Laserna
J J
JJ
D000584:Amiloride / Q000652:urine; D001539:Bendroflumethiazide / Q000652:urine; D002034:Bumetanide / Q000652:urine; D004232:Diuretics / Q000652:urine*; D004300:Doping in Sports; D019075:Electrophoresis, Capillary; D005240:Feasibility Studies; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015813:Substance Abuse Detection; D014223:Triamterene / Q000652:urine
D004232:Diuretics; D002034:Bumetanide; D001539:Bendroflumethiazide; D000584:Amiloride; D014223:Triamterene