[The use of synthetic peptides for detection of antibodies against foot-and-mouth disease virus in the blood from convalescent animals].
Peptides were synthesized, which, according to theoretical analysis of the antigenic structure of protein VP1 of foot-and-mouth disease (FMD) virus types A, 0, and Asia 1, corresponded to potential immunodominant protein sites. Activities of the peptides were studied by solid-phase indirect radioimmunoassay on polyethylene film with purified immunoglobulins against intact FMD virus. Virtually no cross reactions were observed. Blood sera of cattle convalescent after FMD were tested with the FMD virus and peptides containing VP1 fragments 141-160 (A22 No. 550), 140-160 (O1 No. 194), and 140-153 (Asia 1 No. 48). The specificity of interactions between the sera and peptides and the virus was uniform, this permitting the identification of the virus type which caused the disease.
Petrov
V N
VN
Rybakov
S S
SS
Petrova
O N
ON
Lugovskoĭ
A A
AA
Pavlov
A V
AV
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000097:blood*; D036022:Capsid Proteins; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000821:virology; D016056:Immunodominant Epitopes / Q000097:blood*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry
D000956:Antigens, Viral; D036022:Capsid Proteins; D016056:Immunodominant Epitopes; D010455:Peptides