title: Prevalence of single and multiple infection with human papillomaviruses in various grades of cervical neoplasia.
abstract: Evaluation of human papillomavirus (HPV) diversity in various grades of cervical lesions is helpful for understanding the characteristics of HPV infection in the pathogenesis of cervical neoplasia. A total of 227 women with normal cervices (n = 72), low- and high-grade cervical squamous intraepithelial lesions (SILs) (n = 55 and 53, respectively) and cervical carcinomas (n = 47) were screened for human papillomavirus (HPV) types 6, 11, 16 and 18 infection by the polymerase chain reaction. The prevalence of multiple HPV infections in patients with normal cervices, low-grade SILs, high-grade SILs and cervical carcinomas was 22.2%, 61.8%, 41.5% and 21.3%, respectively, while the prevalence of a single-type infection was 36.1%, 21.8%, 30.2% and 61.7%, respectively. HPV 16/11 and 16/18 were the most common combinations observed in multiple infections. Multiple HPV infections were seen most frequently in patients with low-grade SILs, and the prevalence decreased with increasing severity of cervical neoplasia. In contrast, infection with a single HPV type was most commonly observed in patients with cervical carcinoma, and the prevalence decreased with decreasing severity of cervical neoplasia. HPV 16 was the predominant single-type infection in patients with cervical carcinoma and this prevalence decreased steadily with decreasing severity of cervical neoplasia. Conversely, HPV 11 was the predominant single-type infection in patients with normal cervices. This prevalence decreased with increasing severity of cervical neoplasia. Patients with low-grade SILs had a higher prevalence of HPVs, regardless of single or multiple infection status, and larger copy numbers of virus genome were seen more frequently in patients with more severe lesions.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Molecular Medicine, College of Medicine and Hospital, National Taiwan University, Taipei.
authors.forename: D Y
authors.initials: DY
authors.lastname: Chang
authors.forename: R J
authors.initials: RJ
authors.lastname: Chen
authors.forename: S C
authors.initials: SC
authors.lastname: Lee
authors.forename: S C
authors.initials: SC
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000150:complications* / Q000821:virology; D002584:Cervix Uteri / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000150:complications* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000150:complications* / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral