Interleukin-4 stimulates rheumatoid synovial fibroblasts to express matrix metalloproteinase-1 (tissue collagenase) and histamine H1 receptor mRNA.
In the present study, we investigated the effect of interleukin-4 (IL-4) on metalloproteinase 1 (MMP-1/tissue collagenase) production in human rheumatoid synovial fibroblasts. Northern blot analysis revealed that addition of IL-4 with or without histamine stimulated the cells to increase the amount of proMMP-1 mRNA, and the IL-4 with histamine addition resulted in a 3.3-fold increase compared with histamine only. Furthermore, IL-4 itself stimulated the expression of histamine H1 receptor mRNA. These results suggest that IL-4 may play an important role in joint destruction in RA.
Zenmyo
M
M
Department of Pathology, School of Medicine, Kurume University, Japan.
Hiraoka
K
K
Sasaguri
T
T
Komiya
S
S
Inoue
A
A
Morimatsu
M
M
Sasaguri
Y
Y
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis* / Q000235:genetics; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000096:biosynthesis* / Q000235:genetics; D013583:Synovial Membrane / Q000378:metabolism*
D004792:Enzyme Precursors; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D015847:Interleukin-4; D017364:Collagenases; D020781:Matrix Metalloproteinase 1