Folding and stability of the Z and S(iiyama) genetic variants of human alpha1-antitrypsin.
Z (Glu342 --> Lys) and S(iiyama) (Ser53 --> Phe) genetic variations of human alpha1-antitrypsin (alpha1-AT) cause a secretion blockage in the hepatocytes, leading to alpha1-AT deficiency in the plasma. Using in vitro folding analysis, we have shown previously that these mutations interfere with the proper folding of polypeptides. To understand the fundamental cause for the secretion defect of the Z and S(iiyama) variants of alpha1-AT, we investigated in vivo folding and stability of these variant alpha1-AT using the secretion system of yeast Saccharomyces cerevisiae. Various thermostable mutations suppressing the folding block of the Z variant in vitro corrected the secretion defect as well as the intracellular degradation in the yeast secretion system. Significantly, the extent of suppression in the secretion defect of Z protein was proportional to the extent of suppression in the folding defect, assuring that the in vivo defect associated with the Z variant is primarily derived from the folding block. In contrast, the folding and secretion efficiency of S(iiyama) was not much improved by the same mutations. In addition, none of the rarely secreted S(iiyama) alpha1-AT carrying the stabilizing mutations for the wild type and Z variant were active. It appears that the major defect in S(iiyama) variant is the loss of stability in contrast to the kinetic block of folding in the Z variant.
Kang
H A
HA
Division of Protein Engineering, Korea Research Institute of Bioscience and Biotechnology, Yusong, Korea.
Lee
K N
KN
Yu
M H
MH
D006358:Hot Temperature; D006801:Humans; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae; D000515:alpha 1-Antitrypsin / Q000737:chemistry* / Q000648:ultrastructure
D011994:Recombinant Proteins; D000515:alpha 1-Antitrypsin