title: Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.
abstract: The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned, sequenced, expressed, and mapped to kb 2427 on the E. coli chromosome.
authors.affiliation: Institut für Organische Chemie und Biochemie, Technische Universität München, Garching, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Haussmann
authors.forename: F
authors.initials: F
authors.lastname: Rohdich
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: S
authors.initials: S
authors.lastname: Eberhardt
authors.forename: J
authors.initials: J
authors.lastname: Scheuring
authors.forename: S
authors.initials: S
authors.lastname: Mackamul
authors.forename: A
authors.initials: A
authors.lastname: Bacher
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D019798:Neopterin / Q000031:analogs & derivatives; D011621:Pteridines / Q000378:metabolism*; D019998:Racemases and Epimerases / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011621:Pteridines; C013490:dihydroneopterin triphosphate; D019798:Neopterin; D019998:Racemases and Epimerases; C023128:folX protein, E coli