A codominant DNA marker closely linked to the rice nuclear restorer gene, RF-1, identified with inter-SSR fingerprinting.
A new molecular marker (OSRRf) closely linked to the nuclear restorer gene (Rf-1) for fertility in rice has been found. The Rf-1 gene is essential for hybrid rice seed production. A PCR-fingerprinting technique using simple sequence repeats (SSRs) was applied to compare two near-isogenic lines with (MTC-10R) or without (MTC-10A) the Rf-1 gene. Of 76 inter-SSR primers tested, only one primer, (AG)8YC, generated polymorphisms. The tetranucleotide repeats generating polymorphisms were found within each amplicon. the genetic distance between OSRRf and Rf-1 was 3.7 +/- 1.1 cM. As in the case of a codominant marker, this marker will be applied not only to breeding both restorer lines and maintainer lines, but also to the purity management of hybrid rice seeds.
Akagi
H
H
Plant Biotechnology Laboratory, Mitsui Toatsu Chemicals Incorporated, Mobara, Japan.
Yokozeki
Y
Y
Inagaki
A
A
Nakamura
A
A
Fujimura
T
T
D000444:Aldehyde Dehydrogenase / Q000235:genetics; D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins; D016133:Polymerase Chain Reaction
D005819:Genetic Markers; D009687:Nuclear Proteins; D010940:Plant Proteins; D000444:Aldehyde Dehydrogenase