Intralipid rapidly attenuates the agonist-induced activation of rat platelets in vitro.
This study examines the uptake of Intralipid by rat platelets and the effect of Intralipid on platelet activation and platelet lipid composition in vitro. 3H-cholesteryl hexadecyl ether labeled Intralipid (3H-Intralipid) was incubated with rat platelets for up to 4 hours. A significant uptake of 3H-Intralipid occurred as indicated by the increase of 3H in platelets with time. Addition of unlabelled Intralipid or chyle chylomicrons decreased the uptake of labeled Intralipid by the platelets, whereas Intralipid had less effect on the uptake of 14C linoleic acid labeled chylomicrons. Incubation of rat platelets with Intralipid caused a dose dependent inhibition of ADP, collagen and thrombin induced platelet aggregation and serotonin release. Both an attenuated response and a reduction of platelet cholesterol contents was seen within 10 minutes. Intralipid thus causes a rapid, dose- dependent inhibition of agonist-induced activation of rat platelets in vitro. This inhibition is linked to a loss of cholesterol and to an uptake of Intralipid particles by the platelets.
Zhou
L
L
Department of Medicine, University Hospital of Lund, Sweden.
Xu
N
N
Nilsson
A
A
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism*; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D015539:Platelet Activation / Q000187:drug effects*; D051381:Rats
D005217:Fat Emulsions, Intravenous; D002784:Cholesterol