[Site-specific BcuAI endonuclease from Bacillus cereus A].
A new restriction endonuclease was isolated from the Bacillus cereus BKM B-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on DEAE-Sepharose to give about 1400 U of the enzyme per gram of cells. The enzyme revealed the maximum activity at 30-37 degrees C, pH 7.6-8.2, and 5-10 mM MgCl2 under a high ionic strength (50 mM Tris-HCl, 100 mM NaCl). The site-specific endonuclease BcuAI was found to recognize the 5' G decreases G(A/T)CC sequence in double-stranded DNA and cleave it as shown with the arrow, thus being a true isoschisomer of the AvaII restriction endonuclease.
Korolev
S V
SV
Samko
O T
OT
El'darov
M A
MA
Kalugin
A A
AA
Khoroshutina
E B
EB
Omel'ianiuk
N M
NM
Sokolov
N N
NN
Skriabin
K G
KG
D001409:Bacillus cereus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004274:DNA, Recombinant / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis; D013379:Substrate Specificity
D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; C038308:GGWCC-specific type II deoxyribonucleases