Reversed-phase high-performance liquid chromatography method for the analysis of nitro-arginine in rat plasma and urine.
Nitro-L-arginine (L-NNA) is an inhibitor of the enzyme nitric oxide synthase (NOS). We developed a simple, sensitive and reproducible reversed-phase high-performance liquid chromatographic method for detection of nitro-arginine (L- and D-enantiomer) in rat plasma and urine. Samples were treated with perchloric acid, neutralized and eluted through a C8 reversed-phase column with a mobile phase of 18.5 mM heptanesulfonic acid-10% methanol in water, using theophylline as an internal standard. Plasma recovery for both isomers was complete, and the sensitivity limit was 0.5 micrograms/ml. This method may be used for disposition studies of L-NNA in small animals.
Tabrizi-Fard
M A
MA
Department of Pharmaceutics, State University of New York at Buffalo 14260, USA.
Fung
H L
HL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004791:Enzyme Inhibitors / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D019335:Nitroarginine / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D004791:Enzyme Inhibitors; D019335:Nitroarginine; D019001:Nitric Oxide Synthase