Structure and analysis of phospholipase D gene from Ricinus communis L.
Phospholipase D (PLD; EC 3.1.4.4) has been proposed to play a pivotal role in various cellular processes, but molecular understanding of this enzyme is rather limited. This report describes the nucleotide sequence, structure, and genomic organization of a PLD gene from castor bean (Ricinus communis L. cv. Hale). The PLD gene was isolated from a castor bean genomic library using the PLD cDNA as a hybridization probe. Sequence comparison with the PLD cDNA revealed that the PLD gene consisted of four exons and three introns, one of which interrupts the 5'-untranslated region. Southern blot analysis indicated that the cloned PLD gene was present as a single-copy gene, and yet there were other PLD or PLD-related sequences in the castor bean genome.
Xu
L
L
Department of Biochemistry, Kansas State University, Manhattan 66506, USA.
Zheng
L
L
Coughlan
S J
SJ
Wang
X
X
D002367:Ricinus communis / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D018628:Gene Dosage; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics*; D010947:Plants, Toxic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D010739:Phospholipase D