Regional generation of leukotriene C4 after experimental brain injury in anesthetized rats.
Regional concentrations of leukotriene C4 and extravasation of Evans blue were measured after lateral fluid-percussion brain injury in rats. Tissue levels of LTC4 were elevated in the injured cortex at 10 min, 30 min, and 1 h after injury; these levels returned to normal by 2 h after injury. Increases in the levels of LTC4 were also observed in the ipsilateral hippocampus after brain injury, and these elevations persisted for 2 h after injury. No significant increase in levels of LTC4 was observed in the contralateral cortex at any time after injury. A substantial extravasation of Evans blue was observed only in the ipsilateral cortex and hippocampus at 3 h and 6 h after brain injury. Although a temporal association between LTC4 and blood-brain barrier (BBB) breakdown is suggested by these data, no cause-and-effect relationship has been addressed in this study. However, it is possible that, as is true for cerebral ischemia, LTC4 may play a role as a mediator in the BBB breakdown associated with fluid-percussion brain injury in rats.
Dhillon
H S
HS
Department of Surgery, University of Kentucky Chandler Medical Center, Lexington 40536, USA.
Dose
J M
JM
Prasad
M R
MR
D000818:Animals; D001812:Blood-Brain Barrier; D001930:Brain Injuries / Q000378:metabolism*; D005070:Evans Blue; D005260:Female; D017997:Leukotriene C4 / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution; D014949:Wounds, Nonpenetrating / Q000378:metabolism*
D017997:Leukotriene C4; D005070:Evans Blue