Evaluation of the effect of three surface treatments on the biocompatibility of 316L stainless steel using human differentiated cells.
AISI 316L stainless steel (SS) is widely used in orthopaedic implantology, although biological complications may result from its insufficient mechanical and tribological properties. In order to improve the wear and corrosion resistance as well as the hardness of 316L SS, three surface treatments, derived from those applied in mechanical engineering industries, were investigated: (1) glow discharge nitrogen implantation, (2) carbon-doped stainless steel coating sputtering and (3) low temperature plasma nitriding. Surface characterization according to the different heat treatments showed that corrosion and wear resistance were strongly improved, especially by ion implantation or carbon-doped SS coating sputtering. In the same way, microhardness was significantly increased after the three treatments. The effect of such treatments on the biocompatibility of 316L SS was studied with human osteoblast and fibroblast cultures. Basic and specific features of the cells showed that ion-implanted and carbon-doped stainless steels were biocompatible, whereas dramatic cellular reactions were noted when contacted with nitrided stainless steel. A hypothesis is given to explain this observation but further experiments are needed to optimize the nitriding process. Nitrogen implantation and carbon-doped layer deposition could be efficient means for improving the physical properties of stainless steel without affecting its biocompatibility. Such surface treatments may have relevance for increasing the life time of 316L biomedical devices.
Bordji
K
K
Laboratoire de Pharmacologie, URA CNRS 1288, Faculté de Médecine de Nancy, Vandoeuvre-lès-Nancy, France.
Jouzeau
J Y
JY
Mainard
D
D
Payan
E
E
Delagoutte
J P
JP
Netter
P
P
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000592:standards*; D001696:Biomechanical Phenomena; D002244:Carbon / Q000737:chemistry; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005270:Femur Head / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007085:Ilium / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000737:chemistry; D009984:Orthopedic Fixation Devices / Q000592:standards; D010006:Osteoblasts / Q000166:cytology / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D019736:Prostheses and Implants / Q000592:standards*; D011506:Proteins / Q000378:metabolism; D012867:Skin / Q000166:cytology; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D011506:Proteins; D015675:Osteocalcin; D013193:Stainless Steel; D002244:Carbon; D000469:Alkaline Phosphatase; D009584:Nitrogen