title: Novel appearance of placental nuclear monoamine oxidase: biochemical and histochemical evidence for hyperserotonomic state in preeclampsia-eclampsia.
abstract: OBJECTIVE
  The aim of this study was to explore the relevance of placental monoamine oxidase at the subcellular level in the etiology of the hyperserotonomic state in preeclampsia-eclampsia.
  
  
  METHODS
  The study was conducted on placentas from 20 normal pregnant women and 25 women with varied severity of preeclampsia-eclampsia. Placental serotonin and subcellular monoamine oxidase activity were determined. Histochemical localization of monoamine oxidase was done in placental sections and cell isolates.
  
  
  RESULTS
  Placental serotonin increases with severity (rsystolic 0.84, rdiastolic 0.83) and monoamine oxidase decreases (rsystolic 0.86, rdiastolic 0.79). Placental monoamine oxidase showed marked changes in preeclampsia-eclampsia. Histochemical localization of monoamine oxidase showed diffused low activity evenly throughout the cytoplasm and nucleus of the syncytiotrophoblastic cells in preeclampsia-eclampsia; in contrast, normal placenta showed high activity in the cytoplasm without any activity in the nucleus of syncytiotrophoblastic cells. Detection of monoamine oxidase activity in nuclei of the placenta in preeclampsia-eclampsia is a novel finding. Monoamine oxidase activity at the subcellular level further strengthens this observation. A severity-dependent decrease was present in the nuclei of placentas with preeclampsia-eclampsia. The use of specific substrates and inhibitors revealed the presence of monoamine oxidase in mitochondria and nucleus.
  
  
  CONCLUSIONS
  The study delineates an impaired catabolism of placental serotonin in preeclampsia-eclampsia. The novel appearance of monoamine oxidase in nuclei in proximity to its normal site and low activity resulting in a hyperserotonomic state may lead to preeclampsia-eclampsia.
authors.affiliation: Department of Pharmacology, King George's Medical College, Lucknow, India.
authors.forename: V R
authors.initials: VR
authors.lastname: Gujrati
authors.forename: K
authors.initials: K
authors.lastname: Shanker
authors.forename: S
authors.initials: S
authors.lastname: Vrat
authors.lastname: Chandravati
authors.forename: S S
authors.initials: SS
authors.lastname: Parmar
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism*; D004461:Eclampsia / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008995:Monoamine Oxidase / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012701:Serotonin / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012701:Serotonin; D008995:Monoamine Oxidase