Folate absorption in the jejunum of chronic ethanol-fed rats: in vivo studies.
This study sought to determine the intestinal in vivo absorption of folic acid and methyltetrahydrofolate (MTHF) by jejunum surface at different times, after 20 weeks of 30% ethanol ingestion. The absorption results were compared with the data of control rats. In general after ethanol treatment jejunal folic acid absorption was higher than in control rats. When the folic acid concentrations in the perfusion medium were 0.5 microM an increase at later times in ethanol-fed rats was found. At 1 microM the folic acid absorption values were significantly higher at the earlier time. When the concentration assayed was 2.5 microM, significant modifications were only seen at 30 min. Results of MTHF absorption by jejunum of ethanol-fed rats were similar to absorption values of control rats. No significant differences between both groups were found. The results obtained in the present work suggested a different absorptive behavior of both substrates and a different effect of ethanol on folic acid and MTHF absorption in the jejunum.
Fernandez-Borrachero
O
O
Facultad de Farmacia, Universidad de Sevilla, España.
Rubio
J M
JM
Delgado
M J
MJ
Carreras Sánchez
O
O
Murillo
M L
ML
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D000431:Ethanol / Q000008:administration & dosage; D005492:Folic Acid / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013763:Tetrahydrofolates / Q000378:metabolism
D002250:Carbon Radioisotopes; D013763:Tetrahydrofolates; D000431:Ethanol; D005492:Folic Acid; C005984:5-methyltetrahydrofolate