Some properties and protective activity of specific DLE against Salmonella cholerae suis infection.
From a rabbit lymphoid tissue, twice immunized with a Salmonella ch. suis vaccine, was obtained a dialysable leucocyte extract (DLE) (m. w. 10,000Da; protein content 1.14 mg/ml; content of ribose 2.7 mg/ml; A260/A280 ratio 2.17 and pH 6.8). By gel filtration on Sephadex G-25, six peaks were obtained and activity was found in peak IV. The activity of the extract was determined by a dermo-application test (DAT) on 10 cows. The protective effect was tested by a challenge with Salmonella ch. suis and Salmonella dublin pathogen strains on white mice intraperitoneally treated with DLE. The DAT proved to be positive in 8 of the 10 cows. When applied on white mice, it induced a high specific protective effect against Salmonella ch. suis (70%), but not against Salmonella dublin infection.
Arnaudov
A
A
Regional Veterinary Research Institute, Plovdiv, Bulgaria.
Tziporkov
N
N
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology; D002457:Cell Extracts / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003956:Dialysis; D007962:Leukocytes / Q000737:chemistry / Q000276:immunology; D051379:Mice; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D014165:Transfer Factor / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use*
D001428:Bacterial Vaccines; D002457:Cell Extracts; D014165:Transfer Factor