Radiation-enhanced expression of interferon-inducible genes in the KG1a primitive hematopoietic cell line.
X-ray treatment induces a complex molecular response in hematopoietic cells leading to cell death. Using the mRNA differential display technique, we searched for genes whose expression was modified by ionizing radiation (IR) in the human p53-deficient leukemic cell line KG1a. We isolated a partial cDNA corresponding to the interferon (IFN)-inducible 1-8d gene. The expression of both 1-8d and 9-27, another gene from the same IFN-inducible family, was increased 24 and 48 h following irradiation. We did not find enhancement of either IFNgamma mRNA or interferon regulatory factor-1 (IRF-1) mRNA in irradiated KG1a cells, indicating that 1-8d and 9-27 enhancement was not due to an IFN activation. Thus, the induction of IFN-inducible genes by IR may provide a link between radiation-induced and IFN-mediated cell death.
Clave
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Service de Recherches en HématoImmunologie, Laboratoire d'Immunoradiobiologie, DRM-DSV-CEA, Unité de Recherche sur la Biologiedes Cellules Souches, Paris, France.
Carosella
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Gluckman
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Socié
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D001483:Base Sequence; D016923:Cell Death / Q000235:genetics*; D002460:Cell Line / Q000528:radiation effects; D018076:DNA, Complementary / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000032:analysis; D005784:Gene Amplification / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D017781:Genes, Immediate-Early / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D018816:Genes, cdc / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D050856:Interferon Regulatory Factor-1; D007371:Interferon-gamma / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D007371:Interferon-gamma