title: Development of indicators for cephamycin bioassay.
abstract: Several species of bacteria have been tested, for their sensitivity to cephamycin C and other beta-lactam antibiotics with a view to develop an indicator system for identification and quantitation of cephamycin. During the study, a mutant derived from E. coli K 802, exhibited a 10-fold increased sensitivity to cephamycin C than E. coli ESS (reference strain)1,2 and was designated as supersensitive E. coli K 8025. Another interesting feature observed during the investigation was that a strain of Serratia, showed a high degree of resistance to penicillins and cephalosporins however, it was sensitive to cephamycin and its derivative. It has been suggested that E. coli K 8025 and Serratia would serve as good indicator organisms for detection and quantitation of cephamycin.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad, India.
authors.forename: S
authors.initials: S
authors.lastname: Rani
authors.forename: I
authors.initials: I
authors.lastname: Sarada
authors.forename: A
authors.initials: A
authors.lastname: Sircar
authors.forename: P
authors.initials: P
authors.lastname: Sridhar
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001681:Biological Assay; D002513:Cephamycins / Q000032:analysis*; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D002513:Cephamycins; C024925:cephamycin C