Flow cytometric study of the epirubicine accumulation modulation in the human erythrocytes by a Nocardia opaca fraction.
The effect of the "Nocardia lysozyme digest" (NLD) obtained from Nocardia opaca on the Epirubicine accumulation in erythrocytes was studied by flow cytometry. It was shown that the number of fluorescent cells increased under the NLD action due to the increased membrane permeability for epirubicine. An opposite effect was also recorded consisting in the decrease of the relative fluorescence intensity per cell. This effect was attributed to the fluorescence quenching by the oxygen metabolites produced by NDL. In this paper we present some results of the study of the NLD effect on the epirubicine (Epir) accumulation in the erythrocytes (E). It is shown by flow cytometry that the Epir accumulation in the red cells is modified by the presence of the NLD fraction.
Dumitrescu
M
M
Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
Zaharia
C N
CN
Baiu
D
D
Barot-Ciorbaru
R
R
D018943:Anthracyclines / Q000493:pharmacokinetics*; D002457:Cell Extracts / Q000494:pharmacology*; D002463:Cell Membrane Permeability; D004912:Erythrocytes / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D009615:Nocardia / Q000737:chemistry*
D018943:Anthracyclines; D002457:Cell Extracts