Binding of the N-terminal 63 kDa portion of connectin/titin to alpha-actinin as revealed by the yeast two-hybrid system.
Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones coding the C-terminal half region of alpha-actinin (amino acids, 343-897 and 446-897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of alpha-actinin and the N-terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N-terminal 63 kDa portion of connectin binds to alpha-actinin in the Z-line of myofibrillar sarcomeres.
Ohtsuka
H
H
Department of Biology, Faculty of Science, Chiba University, Japan.
Yajima
H
H
Maruyama
K
K
Kimura
S
S
D000185:Actinin / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; D000185:Actinin; D011494:Protein Kinases