Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells.
The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.
Decker
C
C
Department of Physiology, University College London, U.K.
Miro Obradors
M J
MJ
Sillence
D J
DJ
Allan
D
D
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D000440:Butanols / Q000494:pharmacology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology; D020404:Glycerophospholipids; D007668:Kidney; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000440:Butanols; D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; C068564:phosphatidylbutanol; D000431:Ethanol; D002784:Cholesterol; D010739:Phospholipase D; D002794:Choline; D013755:Tetradecanoylphorbol Acetate; D019342:Acetic Acid