Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae.
We have identified a gene in Vibrio cholerae (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model.
Carroll
P A
PA
Infectious Disease Unit, Massachusetts General Hospital, Boston 02114, USA.
Zhao
G
G
Boyko
S A
SA
Winkler
M E
ME
Calderwood
S B
SB
D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D007501:Iron / Q000502:physiology; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics; D011956:Receptors, Cell Surface; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C074789:irgA protein, Vibrio cholerae; D007501:Iron; D000445:Aldehyde Oxidoreductases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C066236:erythrose 4-phosphate dehydrogenase, E coli; D010735:Phosphoglycerate Kinase