Primary human immune response to Neisseria meningitidis serogroup C in interleukin-12-treated severe combined immunodeficient mice engrafted with human peripheral blood lymphocytes.
Lack of primary immune response in severe combined immunodeficient (SCID) mice engrafted with human peripheral blood lymphocytes (hu-PBL) has limited the applicability of this model. Use of human cytokines, in particular interleukin (IL)-12, was studied in the hu-PBL-SCID model. SCID mice were treated with IL-12 and reconstituted with hu-PBL in T replacement factor. The hu-PBL-SCID mice were immunized with serogroup C meningococcal polysaccharide (MCPS). The MCPS-specific antibody response was determined by ELISA. Thirteen of the 15 immunized, IL-12-treated hu-PBL-SCID mice demonstrated a primary human antibody response to MCPS ranging from 0.25 to 3.3 microg/mL, while no MCPS-specific antibody response was detectable in the 18 controls. Expression of cross-reactive idiotypic markers found on human anti-MCPS antibodies in the immunized hu-PBL-SCID mice was similar to that observed in immunized volunteers.
Westerink
M A
MA
Department of Medicine, Medical College of Ohio, Toledo 43699, USA.
Metzger
D W
DW
Hutchins
W A
WA
Adkins
A R
AR
Holder
P F
PF
Pais
L B
LB
Gheesling
L L
LL
Carlone
G M
GM
D000328:Adult; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007114:Immunization; D018664:Interleukin-12 / Q000494:pharmacology*; D007700:Kinetics; D017710:Lymphocyte Transfusion; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009345:Neisseria meningitidis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D016511:Severe Combined Immunodeficiency / Q000276:immunology*; D014183:Transplantation, Heterologous
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000955:Antigens, T-Independent; D011135:Polysaccharides, Bacterial; C037676:meningococcal group C polysaccharide; D018664:Interleukin-12; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin