[The dynamics of 14C-ethanol and 14C-acetaldehyde distribution in rat testes].
Changes in radioactivity were studied during one day in common proteins, karyoplasm and chromatin of testis tissue and in the blood serum of rats after administration of (14C)ethanol and (14C)acetaldehyde. In a series of experiments the acetaldehyde involvement dynamics was determined on the background of disulphiram action. It has been found that the rate of elimination of indicator doses of ethanol and acetaldehyde from the blood varies within a day. In the interval (2-4 h) the ethanol utilization rate is higher than that of acetaldehyde. It is, probably, caused by the activity of ethanol transformation enzymes, intensive ethanol oxidation and inflow of the transformation products to the tissues. Karyoplasm had the highest level of radioactivity among the investigated testis structures. During the day the acetaldehyde accumulation rate per a unit of time was several times lower in chromatin, than in karyoplasm. These results permit supposing that the testis karyoplasm structures possess the mechanisms which inhibit acetaldehyde penetration into chromatin of the male generative cells.
Bozhko
H Kh
HKh
Kraieva
V S
VS
Voloshin
P V
PV
D000079:Acetaldehyde / Q000493:pharmacokinetics*; D000427:Alcohol Deterrents / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004221:Disulfiram / Q000494:pharmacology; D000431:Ethanol / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution / Q000187:drug effects
D000427:Alcohol Deterrents; D002250:Carbon Radioisotopes; D000431:Ethanol; D000079:Acetaldehyde; D004221:Disulfiram