title: Influence of interleukin 1 beta on tenascin distribution in human normal and osteoarthritic cartilage: a quantitative immunohistochemical study.
abstract: OBJECTIVE
  To determine the influence of IL-1 beta on the presence and the distribution of tenascin in matrix of human normal and osteoarthritic cartilage explants.
  
  
  METHODS
  Cartilage was grown in organotypic culture with or without IL-1 beta (10 ng ml1). Tenascin antigen was detected on cryopreserved cartilage sections by immunohistochemical techniques with a monoclonal antibody directed against all tenascin isoforms (BC-4), and then quantified by video imaging densitometry.
  
  
  RESULTS
  Tenascin was present in normal cartilage explants and increased in osteoarthritic cartilage explants. Treatment of normal and osteoarthritic cartilage explants with IL-1 beta (10 ng ml-1) induced an increase in tenascin content, which was particularly high in normal cartilage and predominated in the superficial layers of damaged cartilage. There was no obvious correlation between proteoglycan loss and presence of tenascin.
  
  
  CONCLUSIONS
  In human normal and osteoarthritic cartilage explants, the presence and the distribution of tenascin are influenced by IL-1 beta.
authors.affiliation: Department of Rheumatology, Henri-Mondor hospital, Creteil, France.
authors.forename: X
authors.initials: X
authors.lastname: Chevalier
authors.forename: P
authors.initials: P
authors.lastname: Claudepierre
authors.forename: N
authors.initials: N
authors.lastname: Groult
authors.forename: G J
authors.initials: GJ
authors.lastname: Godeau
mesh_terms: D000368:Aged; D002358:Cartilage, Articular / Q000737:chemistry / Q000378:metabolism*; D046508:Culture Techniques; D003720:Densitometry; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D010003:Osteoarthritis / Q000378:metabolism*; D019063:Tenascin / Q000032:analysis / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D019063:Tenascin