A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: a controlled study.
OBJECTIVE
To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.


METHODS
Controlled clinical study.


METHODS
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.


METHODS
Patients with poor embryo quality in their previous IVF-ET cycles.


METHODS
Embryos from 40 patients, in which > 50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24-48 hours) before being replaced in the uterine cavity.


METHODS
Embryo quality.


RESULTS
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.


CONCLUSIONS
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.
Dirnfeld
M
M
Department of Obstetrics and Gynecology, Carmel Medical Center Haifa, Israel.
Goldman
S
S
Gonen
Y
Y
Koifman
M
M
Calderon
I
I
Abramovici
H
H
D002478:Cells, Cultured; D018920:Coculture Techniques; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000502:physiology*; D006801:Humans; D009924:Organ Culture Techniques; D011247:Pregnancy