Rescue of rinderpest virus from cloned cDNA.
Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that antigenome-sense RNA transcripts of these model genomes can be replicated, transcribed, and packaged by helper virus, both rinderpest virus and the related measles virus. Further, these genome analogs can be replicated and transcribed by viral proteins expressed from cDNA clones by using a recombinant vaccinia virus expressing T7 RNA polymerase (MVA-T7). Using this latter system, we have rescued live rinderpest virus from a full-length cDNA copy of the genome of the RBOK vaccine strain. The recombinant virus appears to grow in tissue culture identically to the original virus.
Baron
M D
MD
Institute for Animal Health, Pirbright, Surrey, United Kingdom. michael.baron@bbsrc.ac.uk
Barrett
T
T
D000818:Animals; D002417:Cattle; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral; D016679:Genome, Viral; D012302:Rinderpest virus / Q000235:genetics*; D014709:Vero Cells
D018076:DNA, Complementary; D004274:DNA, Recombinant; D004279:DNA, Viral