Failure of insulin-like growth factor type I to suppress HIV type 1 in adult or umbilical cord mononuclear blood cells.
Insulin-like growth factor type I (IGF-I) has been used as a treatment for cachexia in adults with AIDS and has been reported to show inhibitory activity against HIV-1IIIB in cord blood mononuclear cells (CBMCs) in vitro at low-concentration (1%) fetal bovine serum (FCS). We evaluated the effect of IGF-I on MN, IIIB, and BaL strains, as well as on a patient isolate of HIV-1 in CBMCs and adult peripheral blood mononuclear cells (PBMCs). IGF-I failed to show any inhibitory effect on HIV replication in CBMCs or adult PBMCs under various culture conditions. In contrast to an earlier report of an antiviral effect, IGF-I augmented HIV-1 replication in PHA-stimulated PBMCs maintained in a low concentration of FCS.
Sharma
U K
UK
Department of Molecular Microbiology and Immunology, School of Public Health an d Hygiene, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Song
H
H
Casella
S J
SJ
Schwartz
D H
DH
D000328:Adult; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D016130:Immunophenotyping; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000821:virology*; D008213:Lymphocyte Activation; D017526:Receptor, IGF Type 1 / Q000276:immunology; D014470:Umbilical Cord / Q000276:immunology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D016655:HIV Core Protein p24; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1