Gene cloning and expression of new trehalose-producing enzymes from the hyperthermophilic archaeum Sulfolobus solfataricus KM1.
The genes encoding for trehalose-producing enzymes, a glycosyl-trehalose-producing enzyme (glycosyltransferase) and a gylcosyl-trehalose-hydrolyzing enzyme (alpha-amylase), from Sulfolobus solfataricus KM1 were cloned and expressed in E. coli. The nucleotide sequence of the glycosyltransferase gene and the alpha-amylase gene indicated proteins with lengths of 728 and 558 amino acids and molecular masses of 86-kDa and 65-kDa, respectively. Regions highly conserved in the alpha-amylase family exist in the amino acid sequences of these enzymes.
Kobayashi
K
K
Applied Bioresearch Center, Kirin Brewery Co., Ltd, Gunma, Japan.
Kato
M
M
Miura
Y
Y
Kettoku
M
M
Komeda
T
T
Iwamatsu
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016695:Glycosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics*; D014871:Water Microbiology; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics*
D016695:Glycosyltransferases; D000516:alpha-Amylases