[Comparative study of the synthesis and specificity of enterotoxin from Vibrio cholerae O139 serotype using monoclonal antibodies].
Strains of V. cholerae serovar 0139 showed a higher intensity of multiplication and lesser nutritive requirements and produced 2-5 times higher amounts of enterotoxin during in-depth culturing than cholera vibrios of groups O1 and non-O1. R-forms of V. cholerae were characterized by the highest production of toxin. Dot-immunoanalysis and immunoblotting with monoclonal antibodies demonstrated the identity of cholera toxin and enterotoxin of V. cholerae serovar O139. The authors come to a conclusion that selective efficacy of the developed vaccines against diarrhea caused by V. cholerae serovar O139 depends on the presence of homologous O-antigen in their composition.
Fedorova
V A
VA
Alenkina
T V
TV
Gromova
O V
OV
Devdariani
E L
EL
Dzhaparidze
M N
MN
Kosmoenko
O M
OM
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004768:Enterotoxins / Q000096:biosynthesis* / Q000276:immunology; D015151:Immunoblotting; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae