title: A flowcytometric study of CD4+ & CD19+ cell lymphopenia in patients with alopecia areata.
abstract: Twenty five patients with alopecia areata and 20 healthy controls were studied by flowcytometry employing direct two colour immunofluorescence in erythrocyte lysed whole blood. A significant reduction was observed in helper/inducer (CD4+) lymphocytes (29.4 +/- 7.4 vs 39.45 +/- 8.0, P < 0.01) and B (CD 19+) lymphocytes (11.04 +/- 6.57 vs 15.0 +/- 5.05, P < 0.01) in comparison with healthy controls. Decrease in T-lymphocytes (CD3+) and suppressor/cytotoxic (CD8+) was not significant and activated (HLADR+) lymphocytes and natural killer cells (CD16+ and CD56+) were within normal limits. Our findings suggest a significant T helper (CD4+) and B (CD19+) cell lymphopenia in alopecia areata.
authors.affiliation: Department of Dermatology, Postgraduate Institute of Medical Education & Research, Chandigarh.
authors.forename: V K
authors.initials: VK
authors.lastname: Sharma
authors.forename: N
authors.initials: N
authors.lastname: Agnihotri
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: H
authors.initials: H
authors.lastname: Vohra
mesh_terms: D000293:Adolescent; D000328:Adult; D000506:Alopecia Areata / Q000276:immunology*; D018941:Antigens, CD19 / Q000097:blood*; D015704:CD4 Antigens / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008231:Lymphopenia / Q000276:immunology*; D008297:Male
chemical_list: D018941:Antigens, CD19; D015704:CD4 Antigens