DNA in situ hybridization for the rapid diagnosis of massive necrotizing avian adenovirus hepatitis and pancreatitis in chicks.
The present study describes the use of DNA in situ hybridization for the rapid diagnosis of massive necrotizing adenovirus hepatitis and pancreatitis in broiler chicks. A light microscope and DNA probes were used to identify avian adenovirus in replicate sections of formalin-fixed paraffin-embedded liver and pancreas from field and experimental chicks. Avian adenovirus infection was confirmed by transmission electron microscopy and virus isolation.
Goodwin
M A
MA
Georgia Poultry Laboratory, Oakwood 30566, USA.
Latimer
K S
KS
Resurreccion
R S
RS
Miller
P G
PG
Campagnoli
R P
RP
D000257:Adenoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000175:diagnosis*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D008099:Liver / Q000737:chemistry / Q000473:pathology / Q000821:virology; D008854:Microscopy, Electron / Q000379:methods / Q000662:veterinary; D010179:Pancreas / Q000737:chemistry / Q000473:pathology / Q000821:virology; D010195:Pancreatitis / Q000175:diagnosis / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis*
D004279:DNA, Viral