title: Cloning and nucleotide sequencing of the protease gene of Vibrio vulnificus.
abstract: The gene (vvp) encoding a thermolabile protease of Vibrio vulnificus was cloned and sequenced. The transcription start point was also determined by primer extension. The product of this gene is very likely the secretory neutral metalloprotease that has been purified and characterized previously.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: J C
authors.initials: JC
authors.lastname: Cheng
authors.forename: C P
authors.initials: CP
authors.lastname: Shao
authors.forename: L I
authors.initials: LI
authors.lastname: Hor
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D014733:Vibrio / Q000201:enzymology / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D008666:Metalloendopeptidases; C070101:vvP protein, Vibrio vulnificus