Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
Three cDNA clones encoding NG,NG-dimethylarginine dimethylamino-hydrolase (EC 3.5.3.18) have been isolated from a rat kidney lambda gt11 library using a probe prepared by PCR on the basis of partially determined amino-acid sequences of the enzyme. The sequence analyses of the cDNAs established that the 3008 bp cDNA contains 855 bp open reading frame encoding 285 amino acids (M(r) 31414), 3'-flanking region (1722 bp) and 5'-flanking region (431 bp). The amino-acid sequences of all the peptides isolated from the purified enzyme were shown to be included in the deduced amino-acid sequence. Northern blotting analyses showed that the mRNA (4 kb) is ubiquitously expressed in various rat tissues.
Kimoto
M
M
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Sasakawa
T
T
Tsuji
H
H
Miyatake
S
S
Oka
T
T
Nio
N
N
Ogawa
T
T
D000581:Amidohydrolases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006867:Hydrolases / Q000235:genetics*; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger; D006867:Hydrolases; D000581:Amidohydrolases; C059999:dimethylargininase