Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.
A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR1-410, the SMR1B gene was confirmed as a convenient dominant selective marker for yeast transformation including industrial strains.
Xie
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Centro de Biología Molecular Severo Ochoa (UAM/CSIC), Universidad Autónoma, Canto Blanco, Madrid, Spain.
Jiménez
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D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D006540:Herbicides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D013453:Sulfonylurea Compounds / Q000494:pharmacology*
D017931:DNA Primers; D005819:Genetic Markers; D006540:Herbicides; D013453:Sulfonylurea Compounds; C042031:sulfometuron methyl