title: Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot springs in Naples, Italy.
abstract: Six slightly halophilic, thermophilic bacterial strains were isolated from saline hot springs along the Bay of Naples, Italy. These strains produce bright yellow colonies and have a filamentous morphology and an optimum growth temperature of about 60 degrees C. Lipid composition and 16S ribosomal DNA sequence analyses showed that these strains belong to the genus Thermonema, a member of the cytophaga-flavobacter-bacteroides phylum. Growth was observed in medium containing 1 to 3% NaCl. The DNA G + C content was 50.9 mol%. DNA-DNA hybridization studies showed that these strains represent a new species of the genus Thermonema. We propose that strain NR-27T (T = type strain) and the other strains represent a new species, Thermonema rossianum. Strain NR-27 (= DSM 10300) is the type strain of this species.
authors.affiliation: Departamento de Bioquímica, Universidade de Coimbra, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Tenreiro
authors.forename: M F
authors.initials: MF
authors.lastname: Nobre
authors.forename: F A
authors.initials: FA
authors.lastname: Rainey
authors.forename: C
authors.initials: C
authors.lastname: Miguel
authors.forename: M S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D001420:Bacteria, Aerobic / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D001431:Bacteriological Techniques; D001482:Base Composition; D003470:Culture Media / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D014812:Vitamin K / Q000032:analysis
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D014812:Vitamin K; D012965:Sodium Chloride