Complementation of a yeast delta pkc1 mutant by the Arabidopsis proteinANT.
The Saccharomyces cerevisiae protein kinase C homologue, PKC1, is involved in maintenance of cell integrity during polarized growth. We have used a mutant complementation approach to investigate related signal transduction pathways in higher plants. Here we report the isolation of a cDNA from Arabidopsis thaliana which partially suppresses the lytic defect of a delta pkc1 yeast strain. The encoded protein, ANT, belongs to the AP2-related gene family and is essential for ovule development. Expression in yeast of a LexA-ANT fusion protein activates transcription of a reporter gene from promoters containing lexA operators. Our results support the idea that ANT acts as transcriptional activator in planta.
Vergani
P
P
Department of Biology, Section of Plant Physiology and Biochemistry, University of Milano, Italy. pbs-pv@wye.ac.uk
Morandini
P
P
Soave
C
C
D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D009875:Operator Regions, Genetic; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion; D012697:Serine Endopeptidases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D000099160:LexA Repressor Protein
D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D010940:Plant Proteins; D011493:Protein Kinase C; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C099055:AINTEGUMENTA protein, Arabidopsis