title: Comparative study of pyridoxine-alpha, beta-glucosides, and phosphopyridoxyl-lysine as a vitamin B6 nutrient.
abstract: The nutritional effects of pyridoxine-alpha-glucoside (PN-alpha-Glc), pyridoxine-5' beta-glucoside (PN-5' beta-Glc) and epsilon-N-(phosphopyridoxyl)-lysine (PNP-Lys) were examined by means of: 1) transport across the intestinal wall using everted rat intestine, 2) metabolic conversion by liver or kidney homogenate, and 3) oral administration of the compound to B6-deficient rats and the subsequent analysis of B6 derivatives found in plasma. Using everted sacs prepared from rat small intestine, PN-alpha-Glc was transported into the serosal side in its intact form. On the other hand, a part of PN-5' beta-Glc was found as PN on the serosal side (PN-5' beta-Glc: PN = 2:1). When PN-alpha-Glc, PN-5' beta-Glc or PNP-Lys was incubated with liver or kidney homogenate for 3 h at pH 6.0, PN-alpha-Glc was hydrolyzed to PN (6%), while there was no hydrolysis of PN-5' beta-Glc. After 30 min of administration of each B6 derivative to B6-deficient rats, blood was collected from the heart, and the B6-derivatives found in plasma were analyzed. It was ascertained that PN-alpha-Glc served as well as PN as a B6 nutrient, while PN-5' beta-Glc and PNP-Lys were not easily metabolized to the coenzyme form, pyridoxal 5'-phosphate.
authors.affiliation: Department of Food Science, Faculty of Agriculture, Gifu University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsuge
authors.forename: M
authors.initials: M
authors.lastname: Maeno
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001692:Biological Transport; D005960:Glucosides; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008239:Lysine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism*; D008297:Male; D011732:Pyridoxal Phosphate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism*; D011736:Pyridoxine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D026681:Vitamin B 6 Deficiency / Q000378:metabolism
chemical_list: D005960:Glucosides; C022945:5'-O-(glucopyranosyl)pyridoxine; D011732:Pyridoxal Phosphate; D008239:Lysine; D011736:Pyridoxine