Nonmuscle myosin heavy chain B is recognized by a monoclonal antibody that inhibits GM1-enhanced neuritogenesis.
Addition of gangliosides to neuroblastoma cells maintained in vitro has been shown to enhance neuritogenesis. Although the mechanism by which they exert this effect is unknown, it has been postulated that they may act by adhering to cell surface proteins. In this article, we describe the isolation and identification of an S20Y murine neuroblastoma cell protein recognized by a monoclonal antibody that was prepared against putative GM1-binding proteins and shown to inhibit GM1-enhanced neuritogenesis. The protein identified was nonmuscle myosin heavy chain B, which appears to function in neurite formation but may not adhere to gangliosides.
Mummert
C M
CM
Cell and Molecular Biology Graduate Program, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
Schengrund
C L
CL
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D007527:Isoenzymes / Q000032:analysis / Q000276:immunology*; D051379:Mice; D018995:Myosin Heavy Chains / Q000032:analysis / Q000276:immunology*; D016501:Neurites / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D009447:Neuroblastoma; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D005677:G(M1) Ganglioside; D018995:Myosin Heavy Chains