Complete coding region of the new HLA-DQB1*0612 allele, obtained by RT-PCR.
A novel HLA-DQB1 allele was detected by oligotyping in the Bubi population of Equatorial Guinea. In order to characterize the new allelic variant, a RT-PCR method which permitted the cloning of its complete coding region was designed. With this method, we have determined the nucleotide sequence of the new DQB1*0612 allele, related to *0604 and *0609 but differing from them at polymorphic codon 70. A proposal for the improvement of the sequencing strategies of HLA class II alleles is made.
Vilches
C
C
Servicio de immunologia, Clinica Puerta de Hierro, Madrid, Spain.
García-Pacheco
J M
JM
de Pablo
R
R
Puente
S
S
Kreisler
M
M
D000483:Alleles; D001483:Base Sequence; D016701:Guinea; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen