[The use of monoclonal antibodies and a biotin-streptavidin system of amplification for the quantitative determination of human IgE].
A "sandwich"-variant of the solid phase immunoenzyme method of quantitative determination of human IgE in biological liquids has been developed on the basis of two monoclonal antibodies to various epitopes of Fc-fragment of E-chain of human IgE and biotin-streptavidin system of the signal amplification. The total analysis time is 3 h: the range of determined concentrations of IgE is 1-200 kE/l; sensitivity-1 kE/l; the type of calibrating curve is linear. To make an analysis twice 10 microliters of the tested sample are required. Reproducibility of the suggested method is not below 5%.
Gordienko
A I
AI
Krivoshein
Iu S
IuS
Malyĭ
K D
KD
Klimovich
V B
VB
Gervazieva
V B
VB
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007139:Immunoglobulin epsilon-Chains / Q000276:immunology; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D019809:Streptavidin
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D007141:Immunoglobulin Fc Fragments; D007139:Immunoglobulin epsilon-Chains; D007202:Indicators and Reagents; D007073:Immunoglobulin E; D001710:Biotin; D019809:Streptavidin