UV-B, UV-A, and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis.
UV and blue light stimulate transcription of key flavonoid biosynthesis genes in a range of higher plants. Here, we provide evidence that several distinct "inductive" and "synergistic" UV/blue phototransduction pathways regulate chalcone synthase (CHS) gene transcription and transcript accumulation in Arabidopsis leaf tissue. Experiments with the long-hypocotyl hy4-2.23N mutant showed that separate inductive pathways mediate responses to UV-B and UV-A/blue light. Only the UV-A/blue light induction of CHS expression involved the CRY1 photoreceptor. In addition, UV-A and blue light each act synergistically with UV-B to stimulate CHS transcript accumulation and beta-glucuronidase activity driven by a CHS promoter in transgenic leaf tissue. The UV-A and blue phototransduction pathways responsible for synergism are distinct because they produce transient and relatively stable signals, respectively, and can function additively to stimulate CHS promoter function. The hy4-2.23N mutant retains the synergistic interactions between UV-B and both UV-A and blue light, indicating that neither synergism pathway involves the CRY1 photoreceptor. Our findings reveal considerable complexity in both photoreception and signal transduction in regulating CHS gene expression by UV and blue light.
Fuglevand
G
G
Division of Biochemistry and Molecular Biology, University of Glasgow, United Kingdom.
Jackson
J A
JA
Jenkins
G I
GI
D000217:Acyltransferases / Q000096:biosynthesis*; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D056931:Cryptochromes; D003624:Darkness; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D005966:Glucuronidase / Q000096:biosynthesis; D008027:Light; D016296:Mutagenesis; D017956:Photoreceptor Cells, Invertebrate; D010940:Plant Proteins / Q000502:physiology; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins / Q000096:biosynthesis; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000528:radiation effects*; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; D010940:Plant Proteins; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; C119828:cry protein, Drosophila; D000217:Acyltransferases; C020141:flavanone synthetase; D005966:Glucuronidase