title: Pathologic study of an experimental canine arthritis induced with Complete Freund's Adjuvant.
abstract: OBJECTIVE
  To describe the physiopathological features of an experimental monoarthritis in a canine model.
  
  
  METHODS
  Pathological changes of the femorotibial joints, periarticular tissues and lymphoid organs were investigated by histology, immunohistochemistry, flow cytometry and cytokine bioassays after intra-articular administration of Complete Freund's Adjuvant in dogs.
  
  
  RESULTS
  An edematous acute synovitis with considerable diapedesis of the neutrophilic granulocytes was observed during a primary response. This was followed by a clinical remission, although a multifocal subacute synovitis was noted with inflammatory infiltrates of mononuclear cells (macrophages > CD8 > CD4). Blood CD14 expression was upregulated and the percentage of CD8+ cells was significantly decreased. Two to four weeks post-induction, a decrease in functional impotency (secondary response) and a pyogranulomatous periarthritis with intramuscular extensions were noted. Focal cartilage erosion was due to adherent granulation tissue (CD4 > CD8 > macrophages).
  
  
  CONCLUSIONS
  In this canine model, experimentally induced arthritis was mainly the result of a cell-mediated immune reaction towards a non-identified antigen.
authors.affiliation: Unité associée INRA de Physiopathologie et Toxicologie Expérimentales, Ecole Nationale Vétérinaire de Toulouse, France.
authors.forename: T
authors.initials: T
authors.lastname: Haak
authors.forename: M
authors.initials: M
authors.lastname: Delverdier
authors.forename: M F
authors.initials: MF
authors.lastname: Amardeilh
authors.forename: I P
authors.initials: IP
authors.lastname: Oswald
authors.forename: P L
authors.initials: PL
authors.lastname: Toutain
mesh_terms: D000208:Acute Disease; D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D015415:Biomarkers; D004195:Disease Models, Animal; D004285:Dogs; D004333:Drug Administration Routes; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000633:toxicity*; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology; D008297:Male; D010641:Phenotype; D013585:Synovitis / Q000473:pathology
chemical_list: D015415:Biomarkers; D005620:Freund's Adjuvant