Serum growth factor regulation of the paired-box transcription factor Pax-3 in neuronal cells.
Mutations in the murine Pax (paired-box)-3 gene results in a number of developmental abnormalities including a deficiency in sensory neurons. To determine the role that Pax-3 plays in neuronal cell development, we have studied the expression of Pax-3 in the ND7 neuronal cell line. Upon serum starvation, Pax-3 mRNA expression fell rapidly. Within 2 h of the removal of serum a 2-3-fold decrease in Pax-3 expression was observed and by 6 h Pax-3 mRNA expression was undetectable. Interestingly this decrease in Pax-3 expression could be reversed by the addition of serum growth factors. This rise in Pax-3 expression upon growth factor addition, was followed by an increase in cell proliferation and a reduction in neurite outgrowth, suggesting that Pax-3 may play a role in mediating cellular responses to these factors in neuronal cells.
Evans
J
J
Division of Biochemistry and Molecular Biology, University of Southampton, UK.
Lillycrop
K A
KA
D000591:Amino Acid Isomerases / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002453:Cell Cycle; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004268:DNA-Binding Proteins / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D019696:Peptidylprolyl Isomerase; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors
D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D004268:DNA-Binding Proteins; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; C068686:Pax3 protein, mouse; D004815:Epidermal Growth Factor; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase