title: High-performance liquid chromatographic determination of baclofen in human plasma.
abstract: A reversed-phase isocratic HPLC method is described for the determination of baclofen in human plasma. Solid-phase extraction using a SCX Bond Elut column is used followed by derivatization with o-phthalaldehyde-tert.-butanethiol and electrochemical detection. Both the within- and between-day R.S.D. and inaccuracy are less than 10% and 7%, respectively, even at the limit of quantification of the method, i.e., 10 ng/ml. The method was shown to give optimum performance in terms of sensitivity, precision and accuracy for the pharmacokinetic study of baclofen after a single oral administration to volunteers.
authors.affiliation: Cephac Research Centre, Saint-Benoît, France.
authors.forename: L
authors.initials: L
authors.lastname: Millerioux
authors.forename: M
authors.initials: M
authors.lastname: Brault
authors.forename: V
authors.initials: V
authors.lastname: Gualano
authors.forename: A
authors.initials: A
authors.lastname: Mignot
mesh_terms: D001418:Baclofen / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D018755:GABA Agonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D018755:GABA Agonists; D007202:Indicators and Reagents; D001418:Baclofen