[Inhibitory effect of ONO-1078, a novel leukotriene receptor antagonist, on human peripheral blood eosinophils].
To determine whether ONO-1078, leukotriene receptor antagonist, inhibits the activation of eosinophils, we investigated the effect of ONO-1078 on the release of leukotriene (LTC4) and eosinophil cationic protein (ECP) from human peripheral blood eosinophils of allergic patients and normal subjects. After preincubation with ONO-1078, eosinophils were incubated with calcium ionophore (A23187). ONO-1078 10(-5) and 10(-6) M inhibited LTC4 release of allergic patients eosinophils and 10(-5)-10(-9) M inhibited LTC4 release of normal subjects eosinophils. ONO-1078, 10(-5) M, also inhibited ECP release of eosinophils obtained from both asthma patients and normal subjects. We think that ONO-1078 may have another pharmacological effect to prevent the activation of human peripheral blood eosinophils by inhibiting LTC4 and ECP release. These results suggest that ONO-1078 might be useful in controlling bronchial asthma.
Kobayashi
S
S
Department of Medicine and Physical Therapy, University of Tokyo.
Yoshida
K
K
Mori
A
A
Sugiyama
H
H
Suko
M
M
Okudaira
H
H
Ito
K
K
Inokuma
S
S
Matsumura
Y
Y
D001249:Asthma / Q000188:drug therapy; D001798:Blood Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology* / Q000627:therapeutic use; D003864:Depression, Chemical; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D006801:Humans; D020024:Leukotriene Antagonists; D017997:Leukotriene C4 / Q000378:metabolism; D008297:Male; D012260:Ribonucleases
D001798:Blood Proteins; D002867:Chromones; D047091:Eosinophil Granule Proteins; D020024:Leukotriene Antagonists; D017997:Leukotriene C4; D012260:Ribonucleases; C047681:pranlukast