Biosynthetically lipid-modified human scFv fragments from phage display libraries as targeting molecules for immunoliposomes.
A human anti-CD22 single chain (sc) Fv antibody fragment from a synthetic phage antibody display library was biosynthetically lipid-tagged by using Escherichia coli lipoprotein sequences. The purified anti-CD22 scFv lipoprotein was incorporated into liposomes by detergent dilution. Anti-CD22 immunoliposomes were shown to bind specifically in a dose- and time-dependent manner to CD22+ cell lines and CD22+ B-lymphocytes present in freshly isolated samples of blood mononuclear cells. The immunoliposomes were demonstrated to accumulate in intracellular compartments. Biosynthetically lipid-tagged human scFv antibody fragments isolated from phage display libraries may facilitate the construction of immunoliposomes with improved properties.
de Kruif
J
J
Department of Immunology, Utrecht University, The Netherlands. j.deKruif@lab.azu.nl
Storm
G
G
van Bloois
L
L
Logtenberg
T
T
D015703:Antigens, CD / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D001435:Bacteriophages / Q000235:genetics*; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D005822:Genetic Vectors; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics*; D007700:Kinetics; D037102:Lectins; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D051918:Sialic Acid Binding Ig-like Lectin 2
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D015815:Cell Adhesion Molecules; D007128:Immunoglobulin Fragments; D037102:Lectins; D008055:Lipids; D008081:Liposomes; D051918:Sialic Acid Binding Ig-like Lectin 2; C055966:immunoglobulin Fv