Interactions between tissues engaged in wound repair and skeletal muscle regeneration.
Enhanced accumulation of granulation tissue in a wound healing model was observed in the presence of regenerating skeletal muscle. This study characterized the tissue accumulated in the normal wound healing and regeneration-adapted models. DNA, RNA, total protein, collagen, and noncollagen protein content, and protein:DNA and noncollagen protein:collagen ratios were determined using standard methods. DNA did not differ; but RNA increased and total protein decreased compared to early granulation tissue. Mean protein: DNA ratio was lower in regeneration-associated granulation tissue compared to age-matched normal and early granulation tissue. Collagen was reduced and noncollagen protein was increased in tissue surrounding regenerating muscle, yielding an elevated noncollagen:collagen ratio. The relative cellularity of granulation tissue was enhanced and collagen deposition potentially reduced, when accompanied by regeneration. Factors from a regeneration environment can interact with cells engaged in wound repair and potentially alter their behavior.
Sicard
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Department of Surgery, University of Minnesota, Minneapolis 55455, USA. sicar001@maroon.tc.umn.edu
Mand
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Hwang
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Nguyen
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D000818:Animals; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000378:metabolism; D011161:Porifera / Q000378:metabolism; D019736:Prostheses and Implants; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014945:Wound Healing / Q000502:physiology*
D011506:Proteins; D012313:RNA; D011142:Polyvinyl Alcohol; D003094:Collagen; D004247:DNA