Rapid method for isolation of high molecular weight plasmids suitable for digestion with restriction endonucleases.
The described method is a modification of the hot alkaline method (Kado and Liu, 1981). The modification lies in replacement of SDS with Triton X- 100. The DNA purified by this method was readily digested with restriction endonucleases for a variety of applications, e. g. restriction analysis of plasmid DNA in epidemiological studies, restriction mapping and cloning of high molecular weight plasmids, and for identification of transpozon mutants.
Rychlík
I
I
Veterinary Research Institute, Brno, Czech Republic.
D004262:DNA Restriction Enzymes; D008967:Molecular Biology / Q000379:methods; D008970:Molecular Weight; D010957:Plasmids / Q000302:isolation & purification*
D004262:DNA Restriction Enzymes