[Transmission of sensitive and resistant strains of Mycobacterium tuberculosis in The Netherlands, 1993-1995, studied by means of DNA fingerprinting].
OBJECTIVE
Determination of the contribution of recent transmission of both sensitive and drug-resistant Mycobacterium tuberculosis strains to the number of tuberculosis cases in the Netherlands.


METHODS
Descriptive study.


METHODS
National Institute for Public Health and Environment, Bilthoven, the Netherlands.


METHODS
Since 1993 all isolates of M. tuberculosis in the Netherlands are sent to the national reference laboratory for surveillance purposes. The strains with IS6110 DNA fingerprint, isolated from January 1993 to July 1995, were analysed for clustering of DNA patterns (clustering of identical DNA patterns was assumed to represent recent transmission of tuberculosis). A transmission index was calculated from the ratio of the number recently infected tuberculosis patients and the number of source patients.


RESULTS
Among 2,217 M. tuberculosis isolates, 1,313 unique DNA fingerprints were observed, while 264 DNA patterns occurred more than once. 904 (41%) DNA fingerprints were part of a cluster of identical fingerprints. The mean cluster size was 3.42. The 232 resistant strains showed significantly less clustering (33% versus 42%, p < 0.02) and a smaller transmission index (0.27 versus 0.42, p < 0.02) compared with sensitive strains.


CONCLUSIONS
Recent transmission contributes to the magnitude of the tuberculosis problem in the Netherlands. The epidemiological situation would, however, lead to gradual elimination of the disease were it not for introduction of tuberculosis from other countries. Transmission of resistant strains is relatively limited. Micro-epidemics caused by resistant M. tuberculosis strains were not observed.
van Soolingen
D
D
Afd. Mycobacteriën, Rijksinstituut voor Volksgezondheid en Milieu, Bilthoven.
Lambregts-van Weezenbeek
C S
CS
de Haas
P
P
Veen
J
J
van Embden
J D
JD
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D018432:Drug Resistance, Multiple / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009426:Netherlands / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000635:transmission; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
D004269:DNA, Bacterial