title: Subcellular changes in platelets in acute and subacute African swine fever.
abstract: The morphological changes in platelets in acute and subacute African swine fever (ASF) and their relationship to pathogenesis were studied. Eight pigs were inoculated with a highly virulent strain of African swine fever (Malawi '83) and 14 with a moderately virulent strain (Dominican Republic '78) for ultrastructural study of platelets, monocyte/macrophages and vascular structures in the liver, spleen, lymph node, bone marrow, lung and kidney. Both viruses produced activation and degranulation of platelets from day 3 after inoculation onwards, coinciding with activation of the mononuclear phagocyte system and virus replication in monocyte/macrophages. Platelet aggregation and viscous metamorphosis of platelets were observed at 5 and 7 days after inoculation with the highly virulent strain, coinciding with endothelial alterations, but platelet aggregation was less prevalent and there was no sign of viscous metamorphosis in animals inoculated with the moderately virulent strain. Virions within platelets were observed at the final stage of acute ASF and at 5-7 days after inoculation in subacute ASF. This suggests that platelets assist in disseminating ASF virus within the body, especially in subacute infections.
authors.affiliation: Departmento de Anatomía y Anatomía Patológica, Facultad de Veterinaria, Universidad de Córdoba, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: J
authors.initials: J
authors.lastname: Hervás
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: F C
authors.initials: FC
authors.lastname: de Lara
authors.forename: J
authors.initials: J
authors.lastname: Pérez
authors.forename: P J
authors.initials: PJ
authors.lastname: Wilkinson
authors.forename: M A
authors.initials: MA
authors.lastname: Sierra
mesh_terms: D000208:Acute Disease; D000357:African Swine Fever / Q000097:blood / Q000473:pathology*; D000818:Animals; D001792:Blood Platelets / Q000473:pathology* / Q000648:ultrastructure*; D005260:Female; D008297:Male; D010976:Platelet Count; D013347:Subcellular Fractions / Q000473:pathology* / Q000648:ultrastructure; D013552:Swine