Studies on high-density cultivation of Vero cells with biosilon solid microcarrier.
From eight kinds of microcarriers, Biosilon has been chosen as the most suitable microcarrier for high-density cultivation of African green monkey kidney (Vero) cells. Conditions affecting high-density cultivation were studied with a 500 ml modified Wheaton spinner flask. When cultivation with a 1.5 L CelliGen bioreactor at inoculum density of 4.9 x 10(6)/ml and perfusion volume increased from 0.20 L to 3.65 L gradually, the cell density could reach 2.05 x 10(7)/ml after 22 days of cultivation. When Vesicular Stomatitis Virus (VSV) was inoculated into the cultures of 1.21 x 10(6) ml at the some MOI (multiplicity of infection), the virus titers reached 7 log TCID50/ml and 8 log TCID50/ml, respectively.
Wang
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D
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
Xiao
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C
Chen
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Z
Huang
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Z
D000818:Animals; D019149:Bioreactors; D002448:Cell Adhesion; D002452:Cell Count; D002465:Cell Movement; D002522:Chlorocebus aethiops; D046508:Culture Techniques / Q000379:methods*; D011137:Polystyrenes; D014709:Vero Cells / Q000821:virology; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014776:Virus Cultivation / Q000379:methods
D011137:Polystyrenes; C024075:styrofoam