Cloning and sequencing of rat cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein has a high homology to the catalytic subunits and the 39-kDa associated protein.
Rat liver phosphoribosylpyrophosphate synthetase is a complex aggregate of 34-kDa catalytic subunits (PRS I and II) and 39- and 41-kDa associated proteins (PAP39 and 41). When the rat cDNA encoding PAP41 was isolated, the deduced protein sequence was seen to contain 369 amino acids with a calculated molecular mass of 41130. PAP41 has a 79 and 49% identity with PAP39 and PRSs, respectively. When conservative substitutions are included, PAP41 and the three other components have a 66% homology. PAP41 shares some common features with PAP39 and the two proteins form the PAP subfamily. The mRNA of PAP41 is present in all rat tissues we examined.
Sonoda
T
T
Department of Biochemistry, Chiba University School of Medicine, Japan. cum121j@med.m.chiba-u.ac.jp
Ishizuka
T
T
Kita
K
K
Ishijima
S
S
Tatibana
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D010446:Peptide Fragments / Q000737:chemistry; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; C086305:PAP39; C103898:PAP41; D010446:Peptide Fragments; D011506:Proteins