Protective mechanism of high-density lipoprotein against endotoxemia in chronic alcohol ingestion.
In the present study, we evaluated the role of high-density lipoprotein (HDL) as an endotoxin-binding protein in chronically alcohol-fed rats. Although the blood endotoxin level was significantly elevated in chronic ethanol-loaded rats, compared with control rats, serum tumor necrosis factor (TNF), ALT, and lactate dehydrogenase were not elevated. Serum HDL and its endotoxin-binding capacity were significantly increased in chronic ethanol-loaded rats. When Kupffer cells isolated from control and chronic ethanol-loaded rats were cultured in the medium containing 3 to 30 mg/dl HDL and endotoxin (500 ng/ml), endotoxin uptake and TNF production of Kupffer cells were decreased in proportion to the concentration of HDL in the medium. These results suggest that the increase in endotoxin-binding capacity of HDL may serve as a protective mechanism against endotoxin in chronic ethanol-loaded rats.
Kitano
H
H
3rd Department of Internal Medicine, Nara Medical University, Japan.
Fukui
H
H
Okamoto
Y
Y
Kikuchi
E
E
Matsumoto
M
M
Kikukawa
M
M
Morimura
M
M
Tsujita
S
S
Nagamoto
I
I
Hoppo
K
K
Nakatani
Y
Y
Nakatani
T
T
Tsujii
T
T
D000437:Alcoholism / Q000276:immunology*; D000818:Animals; D019446:Endotoxemia / Q000276:immunology*; D007728:Kupffer Cells / Q000276:immunology; D008075:Lipoproteins, HDL / Q000502:physiology*; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology*; D008111:Liver Function Tests; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D008075:Lipoproteins, HDL; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; C025357:endotoxin binding proteins