Efficient L-serine production from methanol and glycine by resting cells of Methylobacterium sp. strain MN43.
Resting cells of methanol-utilizing microorganisms isolated from soils were examined for L-serine production under conditions in which L-serine-degradation was suppressed. Strain MN43, a facultative methylotrophic bacterium identified as a Methylobacterium sp., was selected for further studies. Under the optimal conditions, 65 mg/ml L-serine was produced by this bacterium from 50 mg/ml glycine and 104 mg/ml methanol in 5 days, with a molar conversion ratio from glycine to L-serine of 93%. This production is the highest so far reported for microbes producing L-serine.
Hagishita
T
T
Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.
Yoshida
T
T
Izumi
Y
Y
Mitsunaga
T
T
D002453:Cell Cycle / Q000502:physiology; D003470:Culture Media; D005998:Glycine / Q000378:metabolism*; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D000432:Methanol / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis*
D003470:Culture Media; D012694:Serine; D005998:Glycine; D000432:Methanol