Production of sodium azide inactivating substance by resistant mutants of Salmonella typhimurium and other bacteria.
We have found that azide-resistant mutants of Salmonella typhimurium and of other bacteria studied produce a substance which inactivates the azide. The production of this substance was proved by the demonstration of a satellite growth of azide-sensitive cells around colonies of azide-resistant mutants and by testing azide inactivating properties of culture filtrates of the azide-resistant strains. The same substance was found to be present in lower concentrations in culture filtrates of wild-type sensitive strains. In both cultures of sensitive strains, it was apparently produced by the resistant mutants and not by the sensitive cells. The substance does not pass across a dialysis membrane and is heat stable. It has a high molecular weight but is not a protein.
Banic
S
S
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia.
D001386:Azides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D004351:Drug Resistance; D012486:Salmonella typhimurium / Q000187:drug effects / Q000378:metabolism*; D019810:Sodium Azide
D001386:Azides; D001426:Bacterial Proteins; D019810:Sodium Azide