title: Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
abstract: Three cDNA clones encoding NG,NG-dimethylarginine dimethylamino-hydrolase (EC 3.5.3.18) have been isolated from a rat kidney lambda gt11 library using a probe prepared by PCR on the basis of partially determined amino-acid sequences of the enzyme. The sequence analyses of the cDNAs established that the 3008 bp cDNA contains 855 bp open reading frame encoding 285 amino acids (M(r) 31414), 3'-flanking region (1722 bp) and 5'-flanking region (431 bp). The amino-acid sequences of all the peptides isolated from the purified enzyme were shown to be included in the deduced amino-acid sequence. Northern blotting analyses showed that the mRNA (4 kb) is ubiquitously expressed in various rat tissues.
authors.affiliation: Department of Nutrition, School of Medicine, University of Tokushima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kimoto
authors.forename: T
authors.initials: T
authors.lastname: Sasakawa
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.forename: S
authors.initials: S
authors.lastname: Miyatake
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: N
authors.initials: N
authors.lastname: Nio
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D000581:Amidohydrolases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006867:Hydrolases / Q000235:genetics*; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D006867:Hydrolases; D000581:Amidohydrolases; C059999:dimethylargininase