title: Purification of IpaC, a protein involved in entry of Shigella flexneri into epithelial cells and characterization of its interaction with lipid membranes.
abstract: Entry of Shigella flexneri into epithelial cells and lysis of the phagosome involve the secreted IpaA-D proteins. A complex containing IpaC and IpaB is able to promote uptake of inert particles by epithelial cells. This suggested that Ipa proteins, either individually or as a complex, might interact with the cell membrane. We have purified IpaC and demonstrated its interaction with lipid vesicles. This interaction is modulated by the pH, which might be relevant to the dual role of Ipa proteins, in induction of membrane ruffles upon entry and lysis of the endosome membrane thereafter.
authors.affiliation: Laboratoire de Chimie Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: De Geyter
authors.forename: B
authors.initials: B
authors.lastname: Vogt
authors.forename: Z
authors.initials: Z
authors.lastname: Benjelloun-Touimi
authors.forename: P J
authors.initials: PJ
authors.lastname: Sansonetti
authors.forename: J M
authors.initials: JM
authors.lastname: Ruysschaert
authors.forename: C
authors.initials: C
authors.lastname: Parsot
authors.forename: V
authors.initials: V
authors.lastname: Cabiaux
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D010713:Phosphatidylcholines; D010743:Phospholipids; C092342:IpaC protein, Shigella; C024990:asolectin