title: Genomic instability in Chinese hamster cells after exposure to X rays or alpha particles of different mean linear energy transfer.
abstract: Evidence has accumulated that radiation induces a transmissible persistent destabilization of the genome, which may result in effects arising in the progeny of irradiated but surviving cells. An enhanced death rate among the progeny of cells surviving irradiation persists for many generations in the form of a reduced plating efficiency. Such delayed reproductive death is correlated with an increased occurrence of micronuclei. Since it has been suggested that radiation-induced chromosomal instability might depend on the radiation quality, we investigated the effects of alpha particles of different LET by looking at the frequency of delayed micronuclei in Chinese hamster V79 cells after cytochalasin-induced block of cell division. A dose-dependent increase in the frequency of micronuclei was found in cells assayed 1 week postirradiation or later. Also, there was a persistent increase in the frequency of dicentrics in surviving irradiated cells. Moreover, we found an increased micronucleus frequency in all of the 30 clones isolated from individual cells which had been irradiated with doses equivalent to either one, two or three alpha-particle traversals per cell nucleus. We conclude that the target for genomic instability in Chinese hamster cells must be larger than the cell nucleus.
authors.affiliation: Department of Radiation Biology, St. Bartholomew's, London, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Manti
authors.forename: M
authors.initials: M
authors.lastname: Jamali
authors.forename: K M
authors.initials: KM
authors.lastname: Prise
authors.forename: B D
authors.initials: BD
authors.lastname: Michael
authors.forename: K R
authors.initials: KR
authors.lastname: Trott
mesh_terms: D000512:Alpha Particles; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002875:Chromosomes / Q000528:radiation effects*; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts; D016678:Genome; D007700:Kinetics; D018499:Linear Energy Transfer; D008168:Lung; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D015162:Micronucleus Tests; D011005:Plutonium; D013997:Time Factors; D014965:X-Rays
chemical_list: D011005:Plutonium