title: Comparison between vaginal tampon and cervicovaginal lavage specimen collection for detection of human papillomavirus DNA by the polymerase chain reaction. The Canadian Women's HIV Study Group.
abstract: The aim of the study was to compare the accuracy of self-administered vaginal tampon (VT) specimens for the detection of human papillomaviruses (HPVs) with that of cervicovaginal lavage specimens (CVL). Two hundred seventy-four paired VT and CVL specimens were collected prospectively from women at risk of sexually transmitted diseases. Specimens were treated and amplified with the polymerase chain reaction (PCR). Each woman served as her own control. One hundred and forty-four of 272 (52.9%) CVLs and 159 of 271 (58.7%) VTs contained HPV DNA sequences (correlation of 88%). The sensitivity and specificity of vaginal tampons reached 93.9% (138/147) and 80.5% (99/123), respectively. HPV typing results were concordant for 99 negative paired samples and 114 paired samples positive for the same type(s) (correlation of 78.9%). It is concluded that these sampling methods collect cells from different areas of the genital epithelium, highlighting the importance of further assessment of the comparative predictive value of HPV detection in each sample.
authors.affiliation: Départment de Microbiologie-Immunologie, Université de Montréal, Québec, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Coutlée
authors.forename: C
authors.initials: C
authors.lastname: Hankins
authors.forename: N
authors.initials: N
authors.lastname: Lapointe
mesh_terms: D000328:Adult; D000554:Ambulatory Care Facilities; D002170:Canada / Q000453:epidemiology; D003430:Cross-Sectional Studies; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D006801:Humans; D052576:Menstrual Hygiene Products / Q000706:statistics & numerical data*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012749:Sexually Transmitted Diseases / Q000453:epidemiology; D013048:Specimen Handling / Q000379:methods*; D007507:Therapeutic Irrigation / Q000706:statistics & numerical data*; D014412:Tumor Virus Infections / Q000175:diagnosis*; D014621:Vagina
chemical_list: D015342:DNA Probes; D004279:DNA, Viral