Effects of retinoids and role of retinoic acid receptors in human thyroid carcinomas and cell lines derived therefrom.
Retinoic acids (RAs), well characterized regulators of proliferation and differentiation, partly re-differentiate follicular thyroid carcinoma cell lines (FTC-133, FTC-238, and HTC-TSHr) as well as SV40-transfected immortalized thyroid cell lines (ori3 and 7751). This is indicated by the stimulation of type I 5'-deiodinase and other differentiation markers. As demonstrated by RT-PCR, electrophoretic mobility shift, and [3H]-retinoic acid binding assays, thyroid carcinoma cell lines express RA receptor mRNAs and functional ligand- and DNA-binding receptor proteins able to mediate RA-dependent signal transduction. Together, these properties make these thyroid-derived cell lines useful in vitro models for studying the effects of an RA re-differentiation therapy of thyroid cancer.
Schmutzler
C
C
Klinische Forschergruppe, University of Würzburg, Germany.
Brtko
J
J
Bienert
K
K
Köhrle
J
J
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D006801:Humans; D007453:Iodide Peroxidase / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000502:physiology*; D012176:Retinoids / Q000494:pharmacology*; D013539:Simian virus 40; D013964:Thyroid Neoplasms / Q000473:pathology*; D014212:Tretinoin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D012176:Retinoids; D014212:Tretinoin; D007453:Iodide Peroxidase