title: Early ultrastructural changes of articular cartilage and synovial membrane in experimental vitamin A-induced osteoarthritis.
abstract: OBJECTIVE
  To evaluate the sequential ultrastructural changes of the articular cartilage and synovial membrane in the earliest phases of the vitamin A model of osteoarthritis (OA) in the rabbit.
  
  
  METHODS
  The superficial layer of the weight bearing zone of the articular cartilage and the synovial membrane from femorotibial joints of 12 osteoarthritic rabbits were evaluated 3, 6, and 9 days after the triggering intraarticular injection of 100,000 i.u. of retinol palmitate. Four uninjected rabbits were used as controls.
  
  
  RESULTS
  At 3 days, ultrastructural changes of chondrocytes could be seen (hypertrophic cells with increased lipid droplets, chondrocytes rich in microfilaments and glycogen, and some degenerating cells) with no evident lesions of the matrix. The synovium was similar to that of the control rabbits. At 6 days, chondrocyte changes seemed almost identical to those of 3 days, while the synovial membrane appeared markedly involved, substituted by a single layer of A-type cells lying on fibrous subsynovial tissue in which lymphocytes, mast cells, and blood vessels could be seen. Conspicuous alterations and necrobiosis of the cartilaginous cells characterized later stages (9 days). The intercellular matrix was mainly made up of amorphous material and bundles of collagen fibers. The synovial membrane was transformed into a thick fibrous tissue partially covered with scattered cells no longer distinguishable as A or B type.
  
  
  CONCLUSIONS
  Our data suggest that in the vitamin A model of OA the initial metabolic changes of the chondrocytes have a pivotal role in determining the relentless cascade of events leading to the full expression of this disease. Moreover, although several studies have been carried out on the early changes in experimental OA, no complete morphological evaluations on the developmental aspect of this model have been available.
authors.affiliation: Institutes of Rheumatology and Human Anatomy, Università degli studi di Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lapadula
authors.forename: B
authors.initials: B
authors.lastname: Nico
authors.forename: F P
authors.initials: FP
authors.lastname: Cantatore
authors.forename: R
authors.initials: R
authors.lastname: La Canna
authors.forename: L
authors.initials: L
authors.lastname: Roncali
authors.forename: V
authors.initials: V
authors.lastname: Pipitone
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000648:ultrastructure*; D007270:Injections, Intra-Articular; D007719:Knee Joint / Q000648:ultrastructure; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced* / Q000473:pathology*; D011817:Rabbits; D013583:Synovial Membrane / Q000648:ultrastructure*; D013997:Time Factors; D014801:Vitamin A / Q000008:administration & dosage
chemical_list: D014801:Vitamin A