title: Direct somatic embryogenesis and transformation in Cicer arietinum L.
abstract: Somatic embryos were induced directly from immature cotyledons of the genotype of chickpea ICC 4918 (annigiri) on B5 medium supplemented with 2,4,5-T or 2,4-D in combination with BA or KN. Successful transformation was achieved via somatic embryogenesis using Agrobacterium tumefaciens strain LBA4404, carrying a binary plasmid vector system containing neomycin phosphotransferase (NPT II) gene as the selectable marker and beta-glucuronidase (GUS) as a reporter gene. Histochemical staining for GUS expression was observed as primary evidence for transformation.
authors.affiliation: Department of Botany, Kakatiya University, Warangal, India.
authors.forename: R V
authors.initials: RV
authors.lastname: Ramana
authors.forename: C
authors.initials: C
authors.lastname: Venu
authors.forename: T
authors.initials: T
authors.lastname: Jayasree
authors.forename: A
authors.initials: A
authors.lastname: Sadanadam
mesh_terms: D018548:Cotyledon / Q000196:embryology*; D007887:Fabaceae / Q000196:embryology* / Q000235:genetics; D018014:Gene Transfer Techniques; D006651:Histocytochemistry; D010946:Plants, Medicinal; D014170:Transformation, Genetic