A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri.
A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barely glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.
Bailey
P C
PC
Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Leicestershire, UK.
Lycett
G W
GW
Roberts
J A
JA
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D015723:Gene Library; D018525:Germination / Q000235:genetics*; D005875:Gibberellins / Q000494:pharmacology; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D012639:Seeds / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018744:DNA, Plant; D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C066246:late embryogenesis abundant protein, plant; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005982:Glutathione Transferase