title: Nucleotide sequence of the gene encoding the viral polymerase of avian pneumovirus.
abstract: We report here the nucleotide sequence of the L gene of avian pneumovirus (APV). This is the second pneumovirus L gene and the second avian paramyxovirus L gene, following that of Newcastle disease virus, to be sequenced. The APV L gene is 6099 nucleotides long and encodes a single large ORF of 2004 amino acids. This makes the APV L protein the smallest to be described for any nonsegmented, negative-strand RNA virus. The protein contains six linear non-contiguous domains, a putative ATP-binding site and four polymerase motifs previously described for the L proteins of negative-strand RNA viruses. Phylogenetic analysis of domain III of 14 different L proteins suggests the pneumoviruses to be as distant in evolutionary terms from the other members of the Paramyxoviridae as are the Filoviridae.
authors.affiliation: Department of Biological Sciences, University of Warwick, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Randhawa
authors.forename: S D
authors.initials: SD
authors.lastname: Wilson
authors.forename: K P
authors.initials: KP
authors.lastname: Tolley
authors.forename: D
authors.initials: D
authors.lastname: Cavanagh
authors.forename: C R
authors.initials: CR
authors.lastname: Pringle
authors.forename: A J
authors.initials: AJ
authors.lastname: Easton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D018111:Pneumovirus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D012367:RNA, Viral / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014422:Turkeys / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins