Comparison of the in vitro effect of adriblastina on induction of SCEs in V79 cells and human peripheral blood lymphocytes.
Induction of sister chromatid exchanges (SCEs) by the cytotoxic antibiotic adriblastina (doxorubicin, 14-hydroxyrubidomycin) of the anthracycline group isolated from Streptomyces peucetius var. caesius by Farmitalia Research Laboratories was tested in vitro at concentrations of 0.01 microgram/ml, 0.1 microgram/ml and 0.2 microgram/ml using V79 cells and human peripheral blood lymphocytes from healthy donors. In comparison with negative control, adriblastina significantly elevated the SCE frequency both in V79 cells and in human peripheral blood lymphocytes. The results obtained by comparing the effect of equivalent adriblastina concentrations on V79 cells and human peripheral blood lymphocytes showed no significant difference in the mutagenicity effect of both of these cell lines to adriblastina.
Szabová
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Institute of Preventive and Clinical Medicine, Department of Genetics, Bratislava, Slovakia.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004317:Doxorubicin / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D012854:Sister Chromatid Exchange
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin