Determination of naloxone in human plasma by high-performance liquid chromatography with coulometric detection.
Naloxone, the analyte and the internal standard, sumatriptan, are extracted from plasma using solid-phase extraction columns. Chromatography and detection are performed using isocratic reversed-phase high-performance liquid chromatography (HPLC) with coulometric end-point detection. The standard curve was linear over the range 0-50 ng/ml of naloxone in plasma. The reproducibility, the coefficient of variation (C.V.) of the method over the range of the standard curve was 6.2-11.2%. The recovery averaged 90.4 +/- 8.9%. A plasma profile following i.v. administration of naloxone in one normal healthy volunteer is presented.
Franklin
M
M
Department of Psychiatry, University of Oxford, UK.
Odontiadis
J
J
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D009270:Naloxone / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D009292:Narcotic Antagonists / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000032:analysis; D018170:Sumatriptan / Q000032:analysis; D014055:Tomography, Emission-Computed; D014662:Vasoconstrictor Agents / Q000032:analysis
D009292:Narcotic Antagonists; D017366:Serotonin Receptor Agonists; D014662:Vasoconstrictor Agents; D009270:Naloxone; D018170:Sumatriptan