Comparison between fluorescence in situ hybridization and conventional cytogenetics for dicentric scoring: a first-step validation for the use of FISH in biological dosimetry.
In this study the suitability of fluorescence in situ hybridization (FISH) for dicentric detection using a commercially available alpha-satellite probe (Oncor) to label centromeres was compared with the conventional technique for the detection of unstable aberrations. A standard FISH protocol was applied for centromere labelling. Dose-response curves using blood samples irradiated in vitro with gamma-rays (60Co) at a dose-rate of 0.1 Gy/min were established using both techniques and compared. No statistical difference was observed between either method. The FISH technique thus allows a correct detection of unstable aberrations when an alpha-satellite DNA probe is used.
Roy
L
L
Institut de Protection et de Sûreté Nucléaire, Département de Protection de la santé de l'Homme, Fontenay-aux-Roses, France.
Sorokine-Durm
I
I
Voisin
P
P
D002503:Centromere / Q000235:genetics*; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016012:Poisson Distribution; D012303:Ring Chromosomes