Instability of Rts1 (drug-resistant factor) replicon: stabilization by DNA fragments derived from Rts1.
Rts1 is a large naturally occurring plasmid which has a kanamycin resistance gene and exhibits various temperature-sensitive phenotypes. A smaller derivative of plasmid, pOK, contains the Rts1 replicon and the kanamycin resistance gene of Rts1. This plasmid, pOK, is much more unstable than Rts1 at 42.5 degrees C. A DNA fragment, G3, 1590 nucleotides long from Rts1 DNA, stabilized pOK completely at 42.5 degrees C but only in the cis configuration. G3 did not change the copy number of pOK. The pOK derivative containing G3 was destabilized by the presence of a compatible plasmid containing G3. G3 has four inverted repeats, two 14-base direct repeats, and three ORFs. Smaller fragment of G3 also had a stabilization effect and these studies showed that the ORF does not play any role in stabilization.
Yonemitsu
H
H
Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA.
Fujihashi
T
T
Higuchi
H
H
Hong
H
H
Morishige
H
H
Mochida
S
S
Kaji
A
A
D001483:Base Sequence; D053938:DNA Fragmentation; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012093:Replicon