[A comparative analysis of the effect of Bordetella pertussis preparations on a primary culture of murine peritoneal macrophages].
The paper deals with comparative analysis of the effect of corpuscular pertussis vaccine (CPV), pertussis dialysate antigen (PDA) and its fraction 2 (fr. 2 PDA) on primary culture of peritoneal macrophages of mice. It is established that CPV possesses the expressed cytotoxic effect that is of dose-dependent character. PDA and 2 (fr. 2 PDA), in contrast to CPV had not such an effect. Only very high doses of preparations (100 micrograms by protein) evoked weak toxic effect on macrophages. Strict direct correlation between cytotoxic effect of pertussis preparations on the primary culture of peritoneal macrophages of mice and their capacity to induce leukolymphocytosis that permits recommending application of these cells to estimate toxicity of pertussis preparations and prediction of potentiality of development of side effects under their introduction.
Tiukavkina
S Iu
SIu
Moskalenko
E P
EP
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology* / Q000633:toxicity; D001886:Bordetella pertussis / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004305:Dose-Response Relationship, Drug; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D010567:Pertussis Vaccine / Q000494:pharmacology* / Q000633:toxicity
D000942:Antigens, Bacterial; D010567:Pertussis Vaccine