Level of ventricular myosin light chains 1 and 2 determined by ELISA in serum of patients with acute myocardial infarction.
The serum level of human ventricular myosin light chains 1 and 2 was determined by competitive ELISA assay in 56 patients, of whom 26 with acute myocardial infarction (18 with simple AMI and 8 with complex AMI), 10 with unstable angina pectoris and 20 healthy subjects. The serum level of human ventricular myosin light chains 1 and 2 (HVMLC1 and HVMLC2) in healthy subjects was of 6-10 ng/ml. Sensitivity of the assay was 10 ng/ml, and the working range was 10-100 ng/ml. In all AMI patients the HVMLC1 serum level exceeded 2-3 times the reference range within the first 24 hours after the onset of chest pain, the mean (+/- ISD) peak concentration was of 45.7 (+/- 13.6) ng/ml (simple AMI) and 64.22 (+/- 18.5 ng/ml) (severe AMI) 48 hours after infarction and it remained significantly higher than the normal value for another 7-10 days after the onset of infarction. The serum HVMLC2 concentration exceeded 10 ng/ml 48 hours after infarction, with a mean (+/- 1SD) peak concentration of 37.8 (+/- 10.4) ng/ml (simple AMI) and 53.8 (+/- 16.1) ng/ml (complex AMI) 90 hours after the onset of infarction and remained at high values for another 4-7 days. In two of ten patients with unstable angina pectoris LC2 increases above 10 ng/ml. The prolonged release of HVMLC1 and HVMLC2 in serum for 4-7 days after the onset of myocardial infarction may facilitate the retroactive detection of infarction, hence the extension of myocardium necrosis.
Costache
L
L
Cantacuzino Institute, Immunochemistry Laboratory, Bucharest, Romania.
Popescu
P
P
Filip
Z
Z
Ilieşiu
D
D
Lazăr
C
C
Nanea
T
T
D003402:Creatine Kinase / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D009211:Myoglobin / Q000032:analysis; D018994:Myosin Light Chains / Q000276:immunology*; D010088:Oxidoreductases / Q000032:analysis; D012680:Sensitivity and Specificity; D000637:Transaminases / Q000032:analysis
D009211:Myoglobin; D018994:Myosin Light Chains; D010088:Oxidoreductases; D000637:Transaminases; D003402:Creatine Kinase