Expression of two cDNAs encoding class I chitinases of rice in Escherichia coli.
Two cDNAs encoding class I chitinases of rice were expressed in Escherichia coli. The cDNAs were fused to the MS2-polymerase gene in an expression vector, pEx31. The fusion proteins, expressed under the control of the lambda PL-promoter, showed the chitinase activity independent of the existence of the hevein domain. The enzymatic hydrolysis of colloidal chitin by the fusion proteins showed that the proteins were endo-type enzymes.
Pan
C H
CH
Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Rhim
S L
SL
Kim
S I
SI
D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005815:Genetic Code; D005822:Genetic Vectors; D012275:Oryza / Q000201:enzymology*
D018076:DNA, Complementary; D002688:Chitinases