Electrophoretic differentiation of soluble egg antigens from Schistosoma mansoni isolates using SDS-PAGE.
In order to detect intraspecies differences among isolates of Schistosoma mansoni from different geographical regions, soluble egg antigens (SEAs) of three isolates were characterized using SDS-PAGE. SEAs were prepared from eggs of a South African (SA), Eastern Caprivian (EC) and Puerto Rican (PR) isolate, separated by SDS-PAGE, stained and the molecular masses of constituent polypeptides determined. Protein profiles differed for all three isolates with the number of polypeptides varying from 25 for the PR isolate to 27 for the EC and SA isolates. Molecular masses of the polypeptides fall within the range of 12.0 kDa to 97.9 kDa. The EC and SA isolates shared ten common polypeptides, the PR and SA isolates five and the PR and EC isolates two. Only a 30.8 kDa and a 12.0 kDa polypeptide were common to all three isolates. These differences may, to some extent, account for regional variations in the morbidity of intestinal schistosomiasis reported for different geographical isolates of S. mansoni.
Pillay
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Department of Microbiology, University of Durban-Westville, South Africa.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008297:Male; D008970:Molecular Weight; D009115:Muridae; D010063:Ovum / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*
D000947:Antigens, Helminth; D015801:Helminth Proteins