title: Apolipoprotein E genotype and amyloid load in Alzheimer disease and control brains.
abstract: We investigated the effect of apolipoprotein E (apoE) genotype on amyloid load in the frontal and cerebellar cortices of 24 patients with definite Alzheimer disease (AD) and 19 controls. Amyloid load was examined by using two methods: 1) acid-extractable amyloid beta-protein (A beta) and insoluble A beta levels of frontal and cerebellar cortices were measured by using enzyme-linked immunosorbent assay, and 2) all types of amyloid plaques and neurofibrillary tangles (NFT) in the frontal cortices were counted after silver staining. Acid-extractable A beta and insoluble A beta levels were higher in AD brains than controls, although there was an overlap between the groups. Acid-extractable A beta and insoluble A beta levels were higher from AD and controls with the apoE epsilon 4 alleles than those without such alleles. However, the differences did not reach statistical significance in AD group. There was no correlation between acid-extractable A beta or insoluble A3 levels and the number of amyloid plaques in AD and control brains. However, insoluble A beta levels correlated positively with the number of NFT in AD brains. Our results show that although apoE epsilon 4 influences the accumulation of A beta, multiple processes may be involved in deposition of A beta in the brain.
authors.affiliation: Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314, USA.
authors.forename: T
authors.initials: T
authors.lastname: Pirttilä
authors.forename: H
authors.initials: H
authors.lastname: Soininen
authors.forename: P D
authors.initials: PD
authors.lastname: Mehta
authors.forename: O
authors.initials: O
authors.lastname: Heinonen
authors.forename: T
authors.initials: T
authors.lastname: Lehtimäki
authors.forename: N
authors.initials: N
authors.lastname: Bogdanovic
authors.forename: L
authors.initials: L
authors.lastname: Paljärvi
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.forename: O
authors.initials: O
authors.lastname: Kosunen
authors.forename: B
authors.initials: B
authors.lastname: Winblad
authors.forename: P
authors.initials: P
authors.lastname: Riekkinen
authors.forename: H M
authors.initials: HM
authors.lastname: Wisniewski
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001057:Apolipoproteins E / Q000235:genetics*; D001923:Brain Chemistry / Q000235:genetics* / Q000502:physiology*; D002525:Cerebellar Cortex / Q000378:metabolism / Q000473:pathology; D005260:Female; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016874:Neurofibrillary Tangles / Q000378:metabolism / Q000473:pathology; D012727:Sex Characteristics
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E