[Immunization with potential Salmonella enteritidis mutants-- 2. Investigations on the attenuation and immunogenicity for mice and young hens].
Adenine-amino acid auxotrophic Salmonella Enteritidis double mutants were investigated for their in vivo stability, attenuation und immunogenicity using NMRI-mice and SPF-chicks. All mutants used were stable in vivo and attenuated. Two mutants were selected for immunization experiments in chickens according to their protective ability after a single intraperitoneal immunization in NMRI-mice using a homologous and heterologous challenge. After two oral immunizations and homologous oral challenge with the S. Enteritidis wild type strain 6403Nalr, a significant difference (p < 0.01) was demonstrated between the mean caecal bacterial counts of immunized and control chicks. Examination of liver samples revealed a significantly lower degree of colonization of internal organs (p < 0.01) in immunized birds. There was no evidence of cross protection against S. Typhimurium using S. Enteritidis mutants.
Martin
G
G
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin Fachbereich 4 Bakterielle Tierseuchen und Bekämpfung von Zoonosen.
Methner
U
U
Steinbach
G
G
Meyer
H
H
D000818:Animals; D001428:Bacterial Vaccines; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D014613:Vaccines, Attenuated
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated