title: Presence of F420-dependent glucose-6-phosphate dehydrogenase in Mycobacterium and Nocardia species, but absence from Streptomyces and Corynebacterium species and methanogenic Archaea.
abstract: A range of organisms known to contain F420 or to be relatives of mycobacteria were examined for F420-dependent glucose-6-phosphate dehydrogenase (FGD) and NADP-dependent glucose-6-phosphate dehydrogenase (NADP-G6PD) activities. All free-growing Mycobacterium species examined (including a virulent Mycobacterium tuberculosis strain) had FGD activities of 0.014-0.418 mumol min-1 mg protein-1, and NADP-G6PD activities of 0.013-0.636 mumol min-1 mg-1. Armadillo-grown Mycobacterium leprae had FGD activity of 0.008 mumol min-1 mg-1, but no detectable NADP-G6PD activity. Nocardia species also had FGD activity (0.088-0.154 mumol min-1 mg-1). Streptomyces and Corynebacterium species had no FGD, but had NADP-G6PD. Methanogenic Archaea had neither activity.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City 52242, USA.
authors.forename: E
authors.initials: E
authors.lastname: Purwantini
authors.forename: T P
authors.initials: TP
authors.lastname: Gillis
authors.forename: L
authors.initials: L
authors.lastname: Daniels
mesh_terms: D003352:Corynebacterium / Q000201:enzymology; D019605:Euryarchaeota / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D009161:Mycobacterium / Q000201:enzymology*; D009166:Mycobacterium leprae / Q000201:enzymology; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009615:Nocardia / Q000201:enzymology*; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology
chemical_list: D009243:NAD; D009249:NADP; C015084:coenzyme F420; D005954:Glucosephosphate Dehydrogenase; D012256:Riboflavin