Automatic high-performance liquid chromatographic method for the determination of diarrhetic shellfish poison.
A rapid HPLC method with fluorescence detection for the determination of okadaic acid (OA) and dinophysistoxin-1 (DTX-1) in mussels and mussel products is presented. For fluorescence labelling of OA and DTX-1, 9-anthryldiazomethane (ADAM) is used. HPLC with a column-switching system is proposed to avoid time-consuming clean-up procedures after derivatization of sample extracts with ADAM. The column-switching system as well as the chromatographic conditions and detection are described.
Hummert
C
C
Institute of Nutrition and Environment, Friedrich-Schiller-University of Jena, Germany.
Shen
J L
JL
Luckas
B
B
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003967:Diarrhea / Q000139:chemically induced*; D004064:Digestive System / Q000737:chemistry; D007202:Indicators and Reagents; D008387:Marine Toxins / Q000032:analysis*; D019319:Okadaic Acid / Q000032:analysis / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis; D013048:Specimen Handling; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1