Effect of isoprinosine on 3'-Azido-3'-deoxythymidine uptake and phosphorylation in human peripheral blood mononuclear cells.
The effect of the immunomodulating agent Isoprinosine on AZT uptake and phosphorylation in human blood monocytes/macrophages was examined. AZT (1-15 microM) uptake in monocytes/macrophages was rapid and dose- and time-dependent. The main metabolite formed after 1 h was AZT-5'-monophosphate. Isoprinosine cotreatment (5-50 microM) had no significant effect on AZT (5 microM) uptake. On the other hand, Isoprinosine increased the formation of AZT-diphosphate metabolites and, more intensively, of AZT-triphosphate (AZT-TP) metabolites after 1 h incubation. Extended exposure (4 h) resulted in complete absence of AZT-TP due to dephosphorylation of these metabolites or blocking of AZT phosphorylation.
Riva
C
C
Groupe de Recherche sur les Cancers du Poumon et des Voies Aériennes, Université Joseph-Fourier, La Tronche, France.
Barra
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000998:Antiviral Agents / Q000378:metabolism / Q000493:pharmacokinetics*; D006801:Humans; D007542:Inosine Pranobex / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D015215:Zidovudine / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics*
D000276:Adjuvants, Immunologic; D000998:Antiviral Agents; D015215:Zidovudine; D007542:Inosine Pranobex