title: Biglycan, decorin, and versican protein expression patterns in coronary arteriopathy of human cardiac allograft: distinctness as compared to native atherosclerosis.
abstract: BACKGROUND
  Histochemical staining has demonstrated previously dramatic deposits of glycosaminoglycans associated with prominent lipid accumulations in thickened vessel walls of allograft coronary arteries. In this study, we characterized the amount, distribution, and types of proteoglycan in the walls of coronary arteries from human cardiac allografts and from native atherosclerotic (NA) controls as part of a strategy to understand the pathogenesis of transplant arteriopathy (TA).
  
  
  METHODS
  We used polyclonal rabbit antibodies against human biglycan, decorin, and versican localize the proteoglycan molecules in standardized transverse sections of the proximal left anterior descending and right coronary arteries. Slides were scored in a blinded fashion for intensity of proteoglycan staining (0 to 6+) and for localization in the vessel walls.
  
  
  RESULTS
  Unique patterns of proteoglycan distribution were present in TA and NA. Biglycan was particularly prominent in intima and evolving atheromata in severely diseased TA coronary arteries, but not in NA. Decorin was present mainly in adventitia of all vessels and in the intima of NA. Prominent versican accumulation occurred in intima and media of TA coronaries, associated with smooth muscle cells and foam cells. There was a reciprocal pattern of biglycan and decorin staining. Versican colocalized with biglycan. Intimal biglycan and versican deposits were positively correlated to the extent of luminal narrowing in TA.
  
  
  CONCLUSIONS
  The distinctive staining patterns for biglycan, decorin and versican in both native and allograft disease indicate that the synthesis and distribution of these proteoglycans are regulated by different local mechanisms in different atheromatous diseases.
authors.affiliation: Department of Pathology, University of British Columbia, Vancouver, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Lin
authors.forename: J E
authors.initials: JE
authors.lastname: Wilson
authors.forename: C R
authors.initials: CR
authors.lastname: Roberts
authors.forename: K J
authors.initials: KJ
authors.lastname: Horley
authors.forename: G L
authors.initials: GL
authors.lastname: Winters
authors.forename: M R
authors.initials: MR
authors.lastname: Costanzo
authors.forename: B M
authors.initials: BM
authors.lastname: McManus
mesh_terms: D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D003324:Coronary Artery Disease / Q000378:metabolism*; D003327:Coronary Disease / Q000378:metabolism*; D003331:Coronary Vessels / Q000737:chemistry*; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D016027:Heart Transplantation; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D008297:Male; D011509:Proteoglycans / Q000032:analysis*; D014184:Transplantation, Homologous; D017539:Tunica Intima / Q000737:chemistry; D053675:Versicans
chemical_list: C546884:BGN protein, human; D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; C508322:VCAN protein, human; D053675:Versicans