title: Rapid effects of steroid hormones on free intracellular calcium in T84 colonic epithelial cells.
abstract: Studies from our laboratory have demonstrated rapid (< 1 min) nongenomic activation of K+ recycling and Na+/H+ exchange by mineralocorticoids in human colonic epithelium, and studies from other laboratories have demonstrated rapid effects of aldosterone on intracellular Ca2+ concentration ([Ca2+]i) in endothelial cells. Here a rapid nongenomic effect of aldosterone on [Ca2+]i is demonstrated in the human colonic epithelial cell line T84. Aldosterone induced a rapid increase in [Ca2+]i within approximately 2 min. The rise in [Ca2+]i after aldosterone appears to result from the activation of a Ca2+ influx pathway, inasmuch as 1) no increase in [Ca2+]i was observed with aldosterone when cells were bathed in Ca(2+)-free Krebs solution and 2) emptying of the intracellular Ca2+ stores by thapsigargin was not enhanced by addition of aldosterone to extracellular Ca(2+)-free solution. In contrast, the Ca2+ response to aldosterone, in the presence of 2 mM Ca2+ in the external bathing solution, was not decreased after intracellular Ca2+ stores were emptied by thapsigargin. Other mineralocorticoid hormones increased [Ca2+]i, whereas the glucocorticoid hydrocortisone failed to increase [Ca2+]i. These results demonstrate the existence of a mineralocorticoid-specific Ca(2+)-signaling pathway in human colonic T84 (crypt) epithelial cells.
authors.affiliation: Wellcome Trust Cellular Physiology Research Unit, Department of Physiology, University College, Cork, Ireland.
authors.forename: C M
authors.initials: CM
authors.lastname: Doolan
authors.forename: B J
authors.initials: BJ
authors.lastname: Harvey
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003106:Colon / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D017136:Ion Transport / Q000187:drug effects
chemical_list: D000450:Aldosterone; D002118:Calcium