The Schizosaccharomyces pombe map1 gene encodes an SRF/MCM1-related protein required for P-cell specific gene expression.
Cells of Schizosaccharomyces pombe undergo mating and meiosis when starved for a nitrogen source. In this process a P and and M cell first mate to generate a diploid zygote, which subsequently enters meiosis and sporulates. The P mating type is controlled by the mat1-Pc gene at the mating type locus, together with a gene called map1. We show that these two functions are required for expression of the P-specific gene map3. We have cloned the map1 gene and show that it encodes a putative MADS-box containing transcription factor related to mammalian Serum Response Factor (SRF) and Saccharomyces cerevisiae MCM1. The Mat1-Pc protein contains a motif characteristic for proteins that interact with MADS-box factors, suggesting that Mat-Pc and Map1 may form a heterodimer that activates the P-specific map3 gene.
Nielsen
O
O
Department of Genetics, University of Copenhagen, Denmark.
Friis
T
T
Kjaerulff
S
S
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics*; D026341:Minichromosome Maintenance 1 Protein; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D026362:Serum Response Factor; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D018398:Homeodomain Proteins; D026341:Minichromosome Maintenance 1 Protein; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D026362:Serum Response Factor; D014157:Transcription Factors; C099943:map1 protein, S pombe