title: Human papillomavirus DNA in non-melanoma skin cancers of a renal transplant recipient: detection of a new sequence related to epidermodysplasia verruciformis associated types.
abstract: The detection of human papillomavirus (HPV) types originally isolated from patients with epidermodysplasia verruciformis (EV) in skin tumors of transplant recipients may point to a role of this HPV subgroup in non-melanoma skin cancer in immunosuppressed people. We analyzed 17 formalin-fixed, paraffin-embedded biopsies of benign or malignant skin tumors of a renal transplant patient with unusually widespread cutaneous carcinomas. Using a nested polymerase chain reaction (PCR), HPV-specific DNA was demonstrated in 11 specimens (65%). Analysis of nine PCR amplification products revealed four different sequences related to EV-associated HPVs. Three sequences occurred only in one lesion. In six samples identical sequences were found that differed from all HPV sequences published to date and may therefore represent a novel EV-HPV type, preliminarily labeled RTRX7. RTRX7 was found in benign, premalignant, and malignant skin lesions. Alignments identified HPV12 as the closest relative of RTRX7, both in the DNA (81% homology) and in the amino acid sequence (84% homology).
authors.affiliation: Department of Dermatology, University of Innsbruck, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Höpfl
authors.forename: G
authors.initials: G
authors.lastname: Bens
authors.forename: U
authors.initials: U
authors.lastname: Wieland
authors.forename: A
authors.initials: A
authors.lastname: Petter
authors.forename: B
authors.initials: B
authors.lastname: Zelger
authors.forename: P
authors.initials: P
authors.lastname: Fritsch
authors.forename: H
authors.initials: H
authors.lastname: Pfister
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D004819:Epidermodysplasia Verruciformis / Q000473:pathology / Q000821:virology; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008545:Melanoma / Q000737:chemistry*; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D004279:DNA, Viral