Small-angle neutron scattering by a strongly denatured protein: analysis using random polymer theory.
Small-angle neutron scattering profiles are presented from phosphoglycerate kinase, in the native form and strongly denatured in 4 M guanidinium chloride (GdnHCl) solution. The data are interpreted using a model in which the excess scattering density associated with the protein is represented as a finite freely jointed chain of spheres. The similarity of the model-derived scattering function to experiment increases asymptotically with the number of spheres. The improvement of the fit obtained with more than approximately 200 spheres (i.e., two residues per sphere) is insignificant. The effects of finite size of the scattering units and of scattering length variation along the polypeptide chain are examined. Improved agreement with experiment is obtained when these effects are taken into account. A method for rapid calculation of the scattering profile of a full, all-atom configuration is examined. It is found that a representation of the chain containing two scattering units per residue, placed at the backbone and side-chain scattering length centroids, reproduces the full, all-atom profile to within 2%.
Petrescu
A J
AJ
Institute of Biochemistry, Bucharest, Romania.
Receveur
V
V
Calmettes
P
P
Durand
D
D
Desmadril
M
M
Roux
B
B
Smith
J C
JC
D005583:Fourier Analysis; D019791:Guanidine; D006146:Guanidines; D007700:Kinetics; D008961:Models, Structural; D008962:Models, Theoretical; D009502:Neutrons; D010735:Phosphoglycerate Kinase / Q000737:chemistry*; D011336:Probability; D011487:Protein Conformation; D011489:Protein Denaturation; D011897:Random Allocation; D011994:Recombinant Proteins / Q000737:chemistry; D012542:Scattering, Radiation
D006146:Guanidines; D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase; D019791:Guanidine