TAF(II)s mediate activation of transcription in the Drosophila embryo.
Mutations in the genes for two highly conserved TAFs, TAF(II)60 and TAF(II)110, reduce transcription of Bicoid-dependent target genes in vivo. By means of several distinct genetic test systems, specific activator-TAF interactions are shown to support both simple and synergistic enhancement of transcription in the embryo. These studies provide in vivo evidence that TAFs can serve as coactivators to receive gene-specific transcriptional activation signals. This genetic system also presents the opportunity to study the function of basal transcription components in regulating development of complex organisms.
Sauer
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F
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Wassarman
D A
DA
Rubin
G M
GM
Tjian
R
R
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D019521:Body Patterning; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D005123:Eye / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators / Q000235:genetics*; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D046911:Macromolecular Substances; D012333:RNA, Messenger; D035381:TATA-Binding Protein Associated Factors; C103338:Taf4 protein, Drosophila; C084976:Taf6 protein, Drosophila; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; C056478:bcd protein, Drosophila