Cloning and characterization of one member of the chalcone synthase gene family from Solanum tuberosum L.
We have isolated a chalcone synthase gene 2 (ST-CHS2) from potato by rapid amplification of cDNA ends by PCR. CHS2 cDNA had high homology to tomato LET-CHS2 (98%), petunia PHCHSJ (94%), potato ST-CHS1B (92%), petunia PHCHSA (92%), and LET-CHS1 (90%) at the overall 389-amino acid level. Genomic hybridization analysis indicated that CHS genes of potato comprise a family of at least six individual members.
Jeon
J H
JH
Department of Biological Science, Korea Advanced Institute of Science and Technology, Taejon, Korea. joungh@geri4680.geri.re.kr
Kim
H S
HS
Choi
K H
KH
Joung
Y H
YH
Joung
H
H
Byun
S M
SM
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D000217:Acyltransferases; C020141:flavanone synthetase