[Modified method of inoculation of hemocultures from patients with suppurative-septic diseases and typhoid-paratyphoid fever].
A modified method of isolating hemocultures in pyoseptic processes is proposed. It consists in combined use of two known methods: inoculation of the blood by in-depth method and preliminary hemolysis of the blood, and use of commercial medium for assessing the sterility as the nutrient base. High efficacy of the proposed method has been demonstrated in experiments with 17 reference strains of microorganisms. The new approach helped improve the isolation rate of opportunistic enterobacteria, nonfermenting gram-negative bacteria, staphylococci, highly demanding streptococci, and other microorganisms similar in nutrient requirements, as well as the agents of typhoid and paratyphoid fever. This permits unifying the methods of investigation of hemocultures in pyoseptic diseases and typhoid-paratyphoid fevers'.
Iskhakova
Kh I
KhI
Vakhidova
Kh M
KhM
Shamsutdinova
L A
LA
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D002648:Child; D003470:Culture Media; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D010284:Paratyphoid Fever / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification; D012485:Salmonella typhi / Q000302:isolation & purification; D018805:Sepsis / Q000382:microbiology*; D013492:Suppuration / Q000382:microbiology*; D014435:Typhoid Fever / Q000382:microbiology*
D003470:Culture Media