Fluorimetric determination of nicorandil in human plasma by a high-performance liquid chromatographic-postcolumn ultraviolet irradiation [corrected] system equipped with on-line back-pressure tubing.
For the determination of nicorandil in plasma, a fluorometric technique using HPLC-postcolumn UV radiation [corrected] has been developed. The chromatographic system consisted of a single pump, photoreactor and on-line back-pressure tubing. The system was suitable for the separation of nicorandil under the present reaction conditions. The calibration graph was linear over the range 6.5-1170 ng ml-1 using an injected volume of 100 microliters. The pretreatment of the plasma samples consisted only of deproteinizing steps by adding perchloric acid. The mean recovery from plasma was 90.2%.
Mawatari
K
K
Department of Analytical Chemistry, Teikyo University, Kanagawa, Japan.
Nakamura
Y
Y
Shimizu
R
R
Sate
S
S
Iinuma
F
F
Watanabe
M
M
D000284:Administration, Oral; D000891:Anti-Infective Agents, Local / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004355:Drug Stability; D005470:Fluorometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008297:Male; D009536:Niacinamide / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D020108:Nicorandil; D009862:Online Systems; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*
D000891:Anti-Infective Agents, Local; D014665:Vasodilator Agents; D009536:Niacinamide; D020108:Nicorandil; D006861:Hydrogen Peroxide