Differential reactivity of a monoclonal antibody directed to the membrane protein of porcine reproductive and respiratory syndrome virus.
A monoclonal antibody (2C12) against the 19 kDa membrane (M) protein of a Canadian isolate of porcine reproductive and respiratory syndrome (PRRS) virus was produced. By indirect immunofluorescence (IIF) cytoplasmic fluorescence was observed in infected cells, but the pattern of fluorescence was generally different and intensity was weaker than that observed using the nucleocapsid protein-directed monoclonal antibody SDOW17. When tested by IIF towards a total of 26 PRRS virus isolates from Canada, 122 isolates from the US and 13 isolates from Europe the 2C12 MAb reacted with all the North American isolates tested including the VR-2332 isolate and the vaccine (RespPRRS) isolate. However no reactivity was observed towards the European isolates tested including the Lelystad virus. This reactivity pattern suggests that the epitope recognized by this MAb on the M protein of PRRS virus appears highly conserved among North American isolates but absent or weakly expressed on European isolates of PRRS virus.
Magar
R
R
Laboratoire d'hygiène vétérinaire et alimentaire, Agriculture et Agroalimentaire Canada, St-Hyacinthe, Québec.
Larochelle
R
R
Nelson
E A
EA
Charreyre
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D002170:Canada; D002213:Capsid / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D005060:Europe; D019084:Fluorescent Antibody Technique, Indirect; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine; D014481:United States
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins