title: A proposal to reclassify Nocardia pinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov.
abstract: The type strain of Nocardia pinensis was the subject of chemotaxonomic and 16S ribosomal DNA sequencing studies. The resultant nucleotide sequence was aligned with the sequences of representatives of the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella, and phylogenetic trees were generated by using the Fitch-Margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. It was evident from the phylogenetic analyses that N. pinensis represents a distinct phyletic line that is most closely associated with the Gordona clade. This genealogical evidence, together with chemotaxonomic and phenotypic data derived from this and previous studies, indicates that N. pinensis merits generic status within the family Nocardiaceae. Therefore, we propose that N. pinensis Blackall et al. 1989 be reclassified as Skermania piniformis gen. nov., comb. nov. The type strain of Skermania piniformis cleaved an array of conjugated substrates based on the fluorophores 7-amino-4-methylcoumarin and 4-methylumbelliferone.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Seoul National University, Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Chun
authors.forename: L L
authors.initials: LL
authors.lastname: Blackall
authors.forename: S O
authors.initials: SO
authors.lastname: Kang
authors.forename: Y C
authors.initials: YC
authors.lastname: Hah
authors.forename: M
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D001431:Bacteriological Techniques; D001482:Base Composition; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D009615:Nocardia / Q000737:chemistry / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004798:Enzymes; D008055:Lipids; D009171:Mycolic Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S