Cell lipids of the Candida lipolytica yeast grown on methanol.
Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA).
Rupcić
J
J
Department of Chemistry and Biochemistry, Faculty of Medicine, University of Rijeka, Croatia.
Blagović
B
B
Marić
V
V
D002175:Candida / Q000737:chemistry* / Q000254:growth & development; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D000432:Methanol
D005227:Fatty Acids; D008055:Lipids; D000432:Methanol