Tissue specificity of spontaneous point mutations in lambda supF transgenic mice.
Transgenic mice carrying multiple copies of a recoverable lambda phage shuttle vector carrying the supF mutation reporter gene (lambda supF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 x 10(-5)), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue-specific differences in genetic instability.
Leach
E G
EG
Department of Therapeutic Radiology, Yale University, Boyer Center for Molecular Medicine, New Haven, Connecticut 06536, USA.
Narayanan
L
L
Havre
P A
PA
Gunther
E J
EJ
Yeasky
T M
TM
Glazer
P M
PM
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D016153:Genes, Suppressor; D008099:Liver / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D017354:Point Mutation; D012343:RNA, Transfer / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012879:Skin Physiological Phenomena
C096275:supF tRNA; D012343:RNA, Transfer