DNA sequences of RAPD fragments in artiodactyls.
A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61-66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site. The third fragment was 93.1% identical to the human type 2 inositol 1,4,5-trisphosphate receptor gene. This fragment had counterparts in white-tailed deer and reindeer; fragments of slightly different size in these species showed high sequence similarity and the size differences were due to varying numbers of dinucleotide microsatellite repeats inside the fragment.
Kostia
S
S
Department of Biosciences, University of Helsinki, Finland.
Palo
J
J
Varvio
S L
SL
D000818:Animals; D001188:Artiodactyla / Q000235:genetics*; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics; D002417:Cattle; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D003670:Deer / Q000235:genetics; D005260:Female; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D019105:Random Amplified Polymorphic DNA Technique; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D012053:Reindeer / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D013045:Species Specificity
D015220:Calcium Channels; D017931:DNA Primers; C506926:ITPR1 protein, human; C506927:ITPR2 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D004247:DNA