title: The mRNA expression of neurocan, a brain-specific chondroitin sulfate proteoglycan, in neoplastic mammary glands in mice.
abstract: In the experiment of mouse transforming growth factor alpha (TGF alpha) gene expression in mammary tumors, various sizes of amplified products by reverse transcriptase-polymerase chain reaction (RT-PCR) using mouse TGF alpha primers were detected in addition to a predicted size in four strains of mice. During the further analysis of these RT-PCR products in mouse mammary tumors, the transcript of neurocan gene was detected in the mammary tumor from SHN mice by the cloning and nucleotide sequence analysis after RT-PCR reaction using mouse TGF alpha primers. The 5'-nucleotide sequence of sequential 246bp in the amplified cDNA of 527bp was completely identical to a middle part of mouse neurocan cDNA sequence, one of the chondroitin-sulfate proteoglycan expressed in the nervous tissue.
authors.affiliation: Laboratory of Functional Anatomy, Faculty of Agriculture, Meiji University, Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Harigaya
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Tsunoda
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000235:genetics*; D002809:Chondroitin Sulfates / Q000032:analysis / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D037181:Lectins, C-Type; D015674:Mammary Neoplasms, Animal / Q000737:chemistry* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D058580:Neurocan; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D058580:Neurocan; D012333:RNA, Messenger; C076817:NCAN protein, human; D002809:Chondroitin Sulfates