Chronic treatment with cyclosporine A in New Zealand rabbit: aortic and erectile tissue alterations.
Transplanted patients frequently present erectile impotence. In order to test any interference by cyclosporine A (CsA), which is commonly used in the post-transplantation management, we investigated the in vitro contractile and relaxant responses of corpus cavernosum and aorta from rabbits chronically treated with CsA. Male New Zealand White rabbits 6 months of age were treated with CsA (25 mg/kg per day s.c.) or solvent (corn oil) for 3 weeks. Descending thoracic aorta and erectile tissue were studied in vitro at the end of treatment. Isometric tension was recorded. In thoracic aorta, noradrenaline (0.1-30 mM) induced a concentration-dependent contraction with no difference between the two groups. Acetylcholine (30 nM-3 mM) produced relaxation (52 +/- 4% at 1 mM) that was significantly reduced in comparison to controls (67 +/- 4%, P < 0.05). ATP (3-10 mM) relaxation was not significantly different (maximal 78 +/- 10% and 62 +/- 12% in CsA-treated and controls). The relaxation produced by sodium nitrite was reduced in CsA-treated rabbits (at 10 mM and 0.1 mM concentrations). In erectile tissue, no significant variation in the response of isolated erectile tissue to the above drugs was observed between CsA-treated and control animals. These data indicate that chronic treatment with CsA in rabbits, despite alteration of the in vitro response of thoracic aorta, does not directly influence the function of penile tissue with relaxants.
Ragazzi
E
E
Department of Pharmacology, University of Padova, Italy.
Meggiato
C
C
Chinellato
A
A
Italiano
G
G
Pagano
F
F
Calabrò
A
A
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D001769:Blood / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010410:Penile Erection; D010413:Penis / Q000187:drug effects*; D011817:Rabbits; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects
D007166:Immunosuppressive Agents; D016572:Cyclosporine