Regulation of epidermal differentiation by a Distal-less homeodomain gene.
The Distal-less-related homeodomain gene Dlx3 is expressed in terminally differentiated murine epidermal cells. Ectopic expression of this gene in the basal cell layer of transgenic skin results in a severely abnormal epidermal phenotype and leads to perinatal lethality. The basal cells of affected mice ceased to proliferate, and expressed the profilaggrin and loricrin genes which are normally transcribed only in the latest stages of epidermal differentiation. All suprabasal cell types were diminished and the stratum corneum was reduced to a single layer. These data indicate that Dlx3 misexpression results in transformation of basal cells into more differentiated keratinocytes, suggesting that this homeoprotein is an important regulator of epidermal differentiation.
Morasso
M I
MI
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Markova
N G
NG
Sargent
T D
TD
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000502:physiology; D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D018983:DNA Footprinting; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology* / Q000648:ultrastructure; D000091344:Filaggrin Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005801:Genes, Homeobox / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010641:Phenotype; D010750:Phosphoproteins / Q000032:analysis; D011401:Promoter Regions, Genetic / Q000502:physiology; D011498:Protein Precursors / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
D015415:Biomarkers; C088334:Distal-less homeobox proteins; D000091344:Filaggrin Proteins; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D014157:Transcription Factors; C064554:loricrin