title: Cloning and characterization of PO22, a pollen-expressed gene in alfalfa.
abstract: Genomic and cDNA clones have been isolated for a new gene (PO22) expressed in mature pollen of alfalfa. PO22 represents a single- or low-copy gene containing 4 introns, 2 transcriptional start sites, and coding for a predicted protein of 340 amino acids with no signal peptide and a coiled coil structure of 39 amino acids near the carboxyl end. The amino acid sequence of PO22 is 38% identical to that of an early nodule-specific gene (ENOD8), also from alfalfa.
authors.affiliation: Department of Crop Science, University of Guelph, Ontario, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Qui
authors.forename: S
authors.initials: S
authors.lastname: Du
authors.forename: L
authors.initials: L
authors.lastname: Erickson
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D007438:Introns; D000455:Medicago sativa / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; C086143:ENOD8 protein, Medicago sativa; C103698:PO22 protein, Medicago sativa; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant