Differential expression of a Mycobacterium tuberculosis protein recognized by a mouse monoclonal antibody.
Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme-linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65 kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65 kDa Mtb protein that is over-expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.
Cummings
P J
PJ
Department of Medical and Research Technology, University of Maryland School of Medicine, Baltimore 21201, USA.
Rowland
S S
SS
Hooper
N E
NE
Schwalbe
R S
RS
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media