[Chemical modification of RNAse from Bacillus intermedius. Effect on the cytotoxic properties of the enzyme].
Modified derivatives of RNAse from Bacillus intermedius differing in the aggregate enzymatic, physico-chemical and physical properties were prepared. The cytotoxic properties of the compounds were tested by the intravital staining of FL cells with neutral red. It was shown that the native enzyme cytotoxicity correlated with the catalytic activity. However, it was found possible to lower the cytotoxicity of the catalytically active compounds by changing the enzyme aggregate properties.
Kurinenko
B M
BM
Davydov
R E
RE
Bulgakova
R Sh
RSh
D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004396:Coloring Agents; D006150:Guanine Nucleotides / Q000378:metabolism*; D008970:Molecular Weight; D009499:Neutral Red; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D013329:Structure-Activity Relationship
D004396:Coloring Agents; D006150:Guanine Nucleotides; D009499:Neutral Red; D012260:Ribonucleases