title: Subregion- and cell type-restricted gene knockout in mouse brain.
abstract: Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.
authors.affiliation: Howard Hughes Medical Institute, Center for Learning and Memory, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Tsien
authors.forename: D F
authors.initials: DF
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Gerber
authors.forename: C
authors.initials: C
authors.lastname: Tom
authors.forename: E H
authors.initials: EH
authors.lastname: Mercer
authors.forename: D J
authors.initials: DJ
authors.lastname: Anderson
authors.forename: M
authors.initials: M
authors.lastname: Mayford
authors.forename: E R
authors.initials: ER
authors.lastname: Kandel
authors.forename: S
authors.initials: S
authors.lastname: Tonegawa
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005865:Gestational Age; D006624:Hippocampus / Q000502:physiology*; D019426:Integrases / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014764:Viral Proteins
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C045073:Cre recombinase; D019426:Integrases