Macrophage inhibitory factor-A3 derived from Mycobacterium avium serovar 2 inhibits candidacidal activity of murine peritoneal macrophages.
Macrophage inhibitory factor-A3 (MIF-A3), a fraction derived from Mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from C57BL/6, C57BL/10, C3H/HeJ and A/J strains of mice. Inhibition of candidacidal activity was demonstrated at MIF-A3 concentrations ranging from 100-400 micrograms/ml in macrophages without additional stimulators (exception C3H/HeJ macrophages) and in macrophages additionally stimulated with 200 U/ml interferon-gamma, 100 ng/ml phorbol myristate acetate and 0.4 ng/ml E. coli lipopolysaccharide from all mouse strains tested. The decreased candidacidal effect produced by MIF-A3 was dose-dependent and appeared greatest in macrophages treated with phorbol myristate acetate and lipopolysaccharide. This effect was neutralized by the addition of goat anti-MIF-A3 antiserum. Macrophages from the Bcgs mouse strains (C57BL/6 and C57Bl/100 were more sensitive to the effect(s) of MIF-A3 than macrophages from the Bcgr mouse strains (C3H/HeJ and A/J).
Hines
M E
ME
Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, Tifton 31793, USA.
Cray
C
C
Elvinger
F
F
Altman
N H
NH
D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies; D002176:Candida albicans; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000494:pharmacology; D006017:Glycolipids / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D006020:Glycopeptides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009162:Mycobacterium avium / Q000737:chemistry*; D016927:Mycobacterium avium subsp. paratuberculosis; D010587:Phagocytosis / Q000187:drug effects; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D016166:Free Radical Scavengers; D006017:Glycolipids; D006020:Glycopeptides; D008070:Lipopolysaccharides; C106012:MIF A3; D013755:Tetradecanoylphorbol Acetate