Proton gradients during salt adaptation of Synechococcus.
The ability of cells to energize their membranes during salt-adaptation as measured by delta pH was studied using the electron spin resonance (ESR) spin probe technique. Salt-adaptated cells have the following features: an increased stability of delta pH across the cytoplasmic membrane and a significantly lower rate of delta pH formation across the thylakoid membrane. Therefore, during salt-adaptation, the cytoplasmic membrane takes over the role of primary energizing membrane from the thylakoid membrane. In contrast to non-adapted cells, delta pH across the thylakoid membrane of salt-grown cells is higher under respiratory than under photosynthetic conditions.
Nitschmann
W H
WH
Membrane Bioenergetics Group, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Packer
L
L
D000222:Adaptation, Physiological; D002462:Cell Membrane / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D003624:Darkness; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008027:Light; D010788:Photosynthesis; D018087:Plastids / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology*; D011522:Protons; D012965:Sodium Chloride / Q000494:pharmacology*
D011522:Protons; D012965:Sodium Chloride; D011189:Potassium Chloride