Partial characterization of a 17 kDa acidic protein, EFP, induced by thiocarbamate in the early flowering phase in Asparagus seedlings.
Asparagus officinalis seedlings treated with either thiocarbamates or carbamates are induced to flower within 2-3 weeks of application. SDS-PAGE and 2-D PAGE resolutions of total soluble proteins of treated seedlings showed the accumulation of an acidic 17 kDa polypeptide. Partial amino acid sequence of the acidic protein showed homology to the wound-responsive A. officinalis PR 1, AoPR1. We named this protein EFP, early flowering protein, owing to its advanced appearance during the early transition from vegetative to floral phase. Carbamates and thiocarbamates that induced more than 80% flowering rates corresponded with increased level of EFP while compounds that are weak flower inducers showed reduced amount of EFP. The observation thus defined the correlationship of EFP to variable flowering rates.
Yeo
D
D
Institute of Physical and Chemical Research (RIKEN), Wako, Saitama, Japan.
Abe
T
T
Abe
H
H
Sakurai
A
A
Takio
K
K
Dohmae
N
N
Takahashi
N
N
Yoshida
S
S
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013859:Thiocarbamates / Q000494:pharmacology*; D014675:Vegetables / Q000187:drug effects* / Q000378:metabolism
D010940:Plant Proteins; D013859:Thiocarbamates