title: Comparison of several mouse and rat monoclonal antibodies against human fibrinogen.
abstract: Six monoclonal antibodies raised against human fibrinogen have been characterized. Mouse monoclonal antibodies were targeted against sequential epitopes on the immunodominant D-domain of fibrinogen and they crossreacted with all molecules containing the D-domain [fibrin, fibrin(ogen)-degradation products]. Their behavior was not influenced by proteolytic degradation of fibrinogen with plasmin. Rat MoAbs were specific for the conformational epitopes on intact fibrinogen. Their reactivities were substantially lower with fibrin(ogen)-degradation products. Degradation of structures on intact fibrinogen was concomitant with the decay of rat MoAbs reactivity. Those structures were presumably on the C-terminal end of fibrinogen alpha chain and/or on the N-terminal end of fibrinogen beta chain.
authors.affiliation: Institute of Haematology and Blood Transfusion, Praha, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Marecek
authors.forename: J E
authors.initials: JE
authors.lastname: Dyr
authors.forename: H
authors.initials: H
authors.lastname: Cajthamlová
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D005260:Female; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D005340:Fibrinogen