Cloning of a new FLO gene from the flocculating Saccharomyces cerevisiae IM1-8b strain.
A flocculation conferring gene was cloned from a genomic library of the flocculating strain Saccharomyces cerevisiae IM1-8b as a 5 kb DNA fragment. The shortest DNA fragment (XbaI-XbaI) able to confer the flocculating phenotype was 3.1 kb. Southern analysis revealed that this gene was not homologous to the already reported FLO1 gene since strong hybridization signals were obtained when chromosomes IV and XII were probed with a digoxygenin-labelled fragment and no signal at all was detected for chromosome I. Partial sequencing data unequivocally ascribed the cloned fragment to chromosome XII. The gene was detected in a variety of S. cerevisiae strains regardless of their being phenotypically flocculating. This gene which, we propose as FLO2, is able to complement the flo1 mutation and is suppressed by suppressors (fsu3) that do not affect other FLO genes.
Sieiro
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C
Department of Microbiology, Faculty of Pharmacy, University of Santiago de Compostela, Spain.
Reboredo
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NM
Blanco
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P
Villa
T G
TG
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics; D003001:Cloning, Molecular; D005427:Flocculation; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic
C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins