Direct radiofluorination of dopamine: 18F-labeled 6-fluorodopamine for imaging cardiac sympathetic innervation in humans using positron emission tomography.
Fluorine-18 labeled fluorodopamine (FDA) was synthesized by the direct fluorination with [18F]F2 [produced by the nuclear reaction 18O(p,n)18F] of dopamine in anhydrous hydrogen fluoride containing boron trifluoride at -65 degrees C. Reverse-phase high-performance liquid chromatography (HPLC) was used to separate [18F]6-FDA from the reaction mixture containing 18F-labeled 2- and 5-FDA. The radiochemical yield of [18F]6-FDA, with respect to [18F]F2, was 10 +/- 2% at the end of the 120-min synthesis from EOB1. The specific activity of [18F]6-FDA at the end of synthesis, 10 +/- 1.5 Ci/mmol, is sufficiently high that the amount of 6-FDA associated with the infusion of a dose of 5 mCi of [18F]6-FDA over 3 min into a 50-kg human (0.5-0.7 microgram/kg/min) is considerably lower than therapeutic doses (2-10 micrograms/kg/min) of dopamine.
Chirakal
R
R
Department of Nuclear Medicine, Chedoke-Mcmaster Hospitals, Hamilton, Ontario, Canada.
Coates
G
G
Firnau
G
G
Schrobilgen
G J
GJ
Nahmias
C
C
D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D005462:Fluorine Radioisotopes; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D007553:Isotope Labeling; D007726:Krypton; D009682:Magnetic Resonance Spectroscopy; D011786:Quality Control; D013564:Sympathetic Nervous System / Q000000981:diagnostic imaging*; D014055:Tomography, Emission-Computed
D005462:Fluorine Radioisotopes; C029253:6-fluorodopamine; D007726:Krypton; D004298:Dopamine