Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.
We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.
Boivin
G
G
Department of Microbiology, Université Laval, Quebec City, Canada.
Handfield
J
J
Murray
G
G
Toma
E
E
Lalonde
R
R
Lazar
J G
JG
Bergeron
M G
MG
D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000956:Antigens, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D015658:HIV Infections / Q000821:virology; D006801:Humans; D009504:Neutrophils / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D004279:DNA, Viral