[A method for specific isolation of verotoxin-producing Escherichia coli colonies].
The described colony immunoblot is a special double membrane-agar technology, which permits the specific isolation of VTEC in biological materials decided VT-positive by EIA or PCR. The produced Verotoxin (VT) is detected on the surface of the lower membrane by help of monoclonal antibodies. A direct allocation of the corresponding VT-expressing E. coli-colonies on the upper membrane is possible. So the isolation and further characterisation of potential VTEC is practicable in materials with a high microbial contamination too. This isolation method is available for the examination of VT-positive faeces and foods. In the case of foods it is necessary to work with an enrichment culture.
Timm
M
M
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachgebiet Bakteriologie in Dessau.
Klie
H
H
Richter
H
H
Perlberg
K W
KW
D001427:Bacterial Toxins / Q000032:analysis* / Q000096:biosynthesis; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D015151:Immunoblotting; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D004768:Enterotoxins; D022622:Shiga Toxin 1