Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes.
Precise comparisons of mammalian gene maps require common anchor loci as landmarks for conserved chromosomal segments. Using a computer script that automates DNA sequence database alignments, we designed 410 evolutionarily conserved primer pair sequences which are specific for anchor locus gene amplification from any mammalian species' DNA. Primer pairs were designed to span introns for polymorphism ascertainment, and to include sufficient exonic sequence (25-400 bp) to allow for gene identification. A total of 318 primer pairs were optimized for domestic cats, and 86% of the sequenced feline PCR products showed homology to the gene of primer origin. A screen of 20 mammals from 11 orders revealed that 35-52% of the 318 primers yielded a single PCR product without further optimization suggesting that nearly 75% can be optimized for any eutherian mammal.
Lyons
L A
LA
Laboratory of Genomic Diversity, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
Laughlin
T F
TF
Copeland
N G
NG
Jenkins
N A
NA
Womack
J E
JE
O'Brien
S J
SJ
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D017931:DNA Primers; D016208:Databases, Factual; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
D017931:DNA Primers; D005819:Genetic Markers