title: Dose dependent of sister chromatid exchanges in humans lymphocytes induced by in vitro alpha-particle irradiation.
abstract: Exposure of human G0 lymphocytes to high-LET particles under different conditions has been seen, unlike low-LET radiations, to be substantially effective in the induction of sister chromatid exchanges (SCE). However, whereas for fast neutrons a linear dose response of SCE has been determined, there is no sign of a dose-response relationship for alpha-particles. A likely reason for this lack of dose dependence may be the irradiation procedure. Therefore, a technique developed in our laboratory to ensure uniformity of irradiation with alpha-particles was used in the present study. Monolayers of 3 h-stimulated lymphocytes were exposed with alpha-particles from 241Am. Underdispersion was found for the cell-to-cell variance of the number of SCE. The dose response of SCE was linear, with a yield of 3.4 SCE per cell and per Gray.
authors.affiliation: Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt and Gesundheit, Neuherberg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schmid
authors.forename: H
authors.initials: H
authors.lastname: Roos
mesh_terms: D000512:Alpha Particles; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D012854:Sister Chromatid Exchange / Q000528:radiation effects*