title: Analysis of L-[methyl-11C]methionine and metabolites in human plasma by an automated solid-phase extraction and a high-performance liquid chromatographic procedure.
abstract: A fully automated method for separation of L-[methyl-11C]Methionine from metabolites in patient plasma was developed. L-[methyl-11C]Methionine was isolated from plasma by solid-phase extraction (SPE). The radioactivity retained on the SPE column was eluted and injected onto the HPLC system for separation of in vivo formed L-[methyl-11C]methionine radiolabeled metabolites. The yield through the isolation procedure and HPLC analysis was greater than 95% with a precision better than 5% (R.S.D.). The calculated rate of L-[methyl-11C]methionine transport into tumor tissue was markedly different with and without compensation for radiolabeled metabolites in patient plasma.
authors.affiliation: Uppsala University PET Centre, University Hospital, Sweden.
authors.forename: K J
authors.initials: KJ
authors.lastname: Lindner
authors.forename: P
authors.initials: P
authors.lastname: Hartvig
authors.forename: C
authors.initials: C
authors.lastname: Akesson
authors.forename: N
authors.initials: N
authors.lastname: Tyrefors
authors.forename: A
authors.initials: A
authors.lastname: Sundin
authors.forename: B
authors.initials: B
authors.lastname: Långström
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D015203:Reproducibility of Results
chemical_list: D008715:Methionine; C038344:methionine methyl ester