Biochemical properties of acetyl- and butyrylcholinesterase in human meningioma.
The structural properties of acetyl-(AChE) and butyrylcholinesterase (BuChE) in meningioma and the possible relationship with brain and plasma were investigated. Meningioma ChEs were extracted with saline and saline-Triton X-100 buffers. The tumor ChE forms were identified by sedimentation analysis, and their amphiphilic/hydrophilic behaviour was assessed by Triton X-114 phase-partitioning and hydrophobic chromatography. Meningioma contained amphiphilic globular AChE dimers (G2A) and monomers (G1A), and hydrophilic BuChE tetramers (G4H). The conversion of G2A into G1A AChE by reduction confirmed their structures. In contrast to the meningioma species, brain G1A AChE forms remained amphiphilic after incubation with alkaline hydroxylamine and phosphatidylinositol-specific phospholipase C (PIPLC). Meningioma G1A and PIPLC-converted G1H, and brain G1A AChE showed similar rate constants for thermal inactivation, and this suggested that the thermal stability of AChE subunits was unaffected by the presence or not of phosphatidylinositol residues. AChE in meningioma and brain did not differ in the interaction with the lectins Con A, LCA, WGA and RCA. BuChE in meningioma and brain bound to a similar extent to Con A, LCA and WGA-Agarose, whereas one-half of BuChE in the tumor, all in plasma and little in brain was fixed by RCA. Therefore, meningioma possesses RCA(+)- and RCA(-)-BuChE, the former predominating in brain and the latter in plasma. It remains to be clarified whether the tumor RCA(+)-BuChE is intrinsic or derived from plasma.
Sáez-Valero
J
J
Departamento de Bioquímica y Biología Molecular A, Universidad de Murcia, Spain.
Vidal
C J
CJ
D000110:Acetylcholinesterase / Q000134:cerebrospinal fluid / Q000737:chemistry*; D002091:Butyrylcholinesterase / Q000134:cerebrospinal fluid / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D017261:Glycosylphosphatidylinositols; D006358:Hot Temperature; D006801:Humans; D046911:Macromolecular Substances; D008579:Meningioma / Q000201:enzymology*; D008970:Molecular Weight; D012995:Solubility
D004220:Disulfides; D017261:Glycosylphosphatidylinositols; D046911:Macromolecular Substances; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase