[Chemotactic activity of neutrophils from atopic and non-atopic subjects--effect of sodium cromoglycate (DSCG)].
It has been well documented, that sodium cromoglycate (DSCG) is capable in inhibiting activity of several inflammatory cells putatively involved in allergic and non-allergic asthmatic inflammation. The goal of this study was to compare the effect of DSCG on random locomotion and chemotaxis of neutrophils to several stimuli in atopic and non-atopic subjects. In 10 seasonal asthmatic (SA), and 10 healthy subjects (HS) chemotactic responses of neutrophils were examined using modified Boyden's microchamber (Neuroprobe) technique. Neutrophils isolated from both HS and SA demonstrated similar spontaneous migration and dose dependent chemotactic responses to FMLP (10(-12) - 10(-5M)), PAF (10(-7) - 10(-5M)) and ZAS (2.5% - 50%). DSCG in concentration range 10(-7) - 10(-9M) expressed a dose-dependent inhibition of both random migration and chemotactic responses to all stimuli tested, with maximal inhibition ranging from 58%-89% and 67%-75% for HS and SA, respectively. Our results confirm potent anti-inflammatory activity of DSCG in vitro, and demonstrate, that this activity is similar in atopic asthmatics, and in healthy subjects.
Szkudlińska
B
B
Katedry i Zakładu Immunologii Klinicznej A. M. w Lodzi.
Kowalski
M L
ML
Grzegorczyk
J
J
Pierzchała
A
A
D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy*; D002633:Chemotaxis / Q000187:drug effects*; D004205:Cromolyn Sodium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects*
D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D004205:Cromolyn Sodium