title: Characterization of the amsI gene product as a low molecular weight acid phosphatase controlling exopolysaccharide synthesis of Erwinia amylovora.
abstract: The ams region, responsible for amylovoran synthesis of the fireblight pathogen Erwinia amylovora, contains the gene amsI encoding a 144 amino acid protein with homology to mammalian low molecular weight acid phosphatases [Bugert and Geider (1995) Mol. Microbiol. 15, 917-9331. A DNA fragment with amsI was cloned under the control of the lac promoter on a high copy number plasmid. The gene product of amsl is about 17 kDa in a protein expression system and had the enzymatic activity of an acid phosphatase. This is the first report about a low molecular weight acid phosphatase activity in prokaryotes. As part of the large ams transcript, expression of amsI was affected by the activator proteins RcsA and RcsB. Overexpression of amsI in E. amylovora caused a strong increase of acid phosphatase activity, but additionally a strong reduction in EPS synthesis, phenotypically similar to a mutation in the gene. The gene product may participate in changes of phosphorylation required for the biosynthesis of EPS such as recycling the lipid carrier diphosphate to the monophosphate form.
authors.affiliation: Max-Planck-Institut für Zellbiologie, Ladenburg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Bugert
authors.forename: K
authors.initials: K
authors.lastname: Geider
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism*; D000180:Acrylic Resins; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D004885:Erwinia / Q000201:enzymology*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
chemical_list: D000180:Acrylic Resins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011135:Polysaccharides, Bacterial; C100769:amylovoran; C016680:polyacrylamide gels; C074889:RcsB protein, Bacteria; C067572:RcsA protein, E coli; D000135:Acid Phosphatase