title: Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase.
abstract: At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase. We show that transcription activation at Class II CAP-dependent promoters requires not only the previously characterized interaction between an activating region of CAP and the RNA polymerase alpha subunit C-terminal domain, but also an interaction between a second, promoter-class-specific activating region of CAP and the RNA polymerase alpha subunit N-terminal domain. We further show that the two interactions affect different steps in transcription initiation. Transcription activation at Class II CAP-dependent promoters provides a paradigm for understanding how an activator can make multiple interactions with the transcription machinery, each interaction being responsible for a specific mechanistic consequence.
authors.affiliation: Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, New Jersey 08855, USA.
authors.forename: W
authors.initials: W
authors.lastname: Niu
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: G
authors.initials: G
authors.lastname: Tau
authors.forename: T
authors.initials: T
authors.lastname: Heyduk
authors.forename: R H
authors.initials: RH
authors.lastname: Ebright
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001665:Binding Sites / Q000235:genetics; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D004563:Electrochemistry; D004789:Enzyme Activation / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D016296:Mutagenesis / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D000596:Amino Acids; D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases