title: Rapid dendritic Ca2+ influx is associated with induction of homosynaptic long-term depression in adult rat hippocampus.
abstract: Homosynaptic long-term depression was induced in area CA1 of adult hippocampus by prolonged low-frequency stimulation (900 pulses at 2 Hz) in the presence of the GABAA receptor antagonist picrotoxin. Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels. This transient increase, by itself, was insufficient for long-term depression induction.
authors.affiliation: Roche Institute of Molecular Biology, Nutley, NJ 07110, USA. otani@pop.u-psud.fr
authors.forename: S
authors.initials: S
authors.lastname: Otani
authors.forename: J A
authors.initials: JA
authors.lastname: Connor
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology; D003712:Dendrites / Q000378:metabolism*; D004558:Electric Stimulation; D058787:GABA-A Receptor Antagonists; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D013569:Synapses / Q000502:physiology*
chemical_list: D002118:Calcium; D015220:Calcium Channels; D058787:GABA-A Receptor Antagonists; D010852:Picrotoxin