title: Expression and localization of annexin V and annexin VI during limb bud formation in the rat fetus.
abstract: We have investigated the expression and the localization of annexin V and annexin VI during the development of rat fetal limb buds by immunoblot and immunocytochemical analysis. Neither annexin V nor annexin VI was detectable in undifferentiated mesenchymal cells in limb buds of the day-13-day-16 rat fetus. Skeletal muscles, whose progenitor cells migrate from the somites and appeared in the limb buds at day 14, dramatically expressed annexin VI on the cell surface after differentiation from mononucleated myogenic cells into multinucleated myotubes. At day 16 both annexin V and annexin VI were found to be expressed in differentiated chondrocytes as well as in the perichondrium, a precursor of chondrocytes, whereas the compact layer of mesenchymal cells surrounding a chondrification center (precartilage) did not show any immunoreactivity for either of these proteins. The results suggest a close relationship between the expression of these annexins and cell differentiation of chondrocytes and skeletal muscles during limb but development.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
authors.forename: H
authors.initials: H
authors.lastname: Iida
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000096:biosynthesis*; D017317:Annexin A6 / Q000096:biosynthesis*; D002356:Cartilage / Q000737:chemistry / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D018878:Limb Buds / Q000196:embryology*; D018613:Microscopy, Confocal; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells
chemical_list: D017304:Annexin A5; D017317:Annexin A6