title: Characterization of Cryptococcus neoformans by random DNA amplification.
abstract: Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Ruma
authors.forename: S C
authors.initials: SC
authors.lastname: Chen
authors.forename: T C
authors.initials: TC
authors.lastname: Sorrell
authors.forename: A G
authors.initials: AG
authors.lastname: Brownlee
mesh_terms: D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal