Re-evaluation of the expression of the gastric H,K-ATPase alpha subunit along the rat nephron.
Under normal conditions, the rat collecting duct displays an H, K-ATPase activity with kinetic and pharmacological properties very close to those of the gastric H,K-ATPase. However, whether the collecting duct H,K-ATPase and the gastric enzyme are identical remains controversial. Therefore, we re-evaluated the expression of the mRNAs encoding the gastric H,K-ATPase alpha subunit in the rat nephron. For this purpose, gastric H,K-ATPase mRNAs were quantitated by RT-PCR at the level of microdissected nephron segments using known amounts of gastric H,K-ATPase cRNA as external standards. Results indicate that gastric H,K-ATPase mRNAs are undetectable (<1 copy per cell) in the glomerulus and along the proximal tubule, thick ascending limb of Henle's loop and collecting duct, although a faint expression ( approximately 400 copies per micro;g total RNA) is measurable in whole-kidney preparations. Gastric H,K-ATPase mRNA is also absent along the nephron of K-depleted rats and of rats with chronic metabolic acidosis and alkalosis. Taken with other data from the literature, these results suggest that the collecting duct of normal rats might express an H,K-ATPase similar, but not identical, to the gastric isoform.
Cheval
L
L
Laboratoire de Biologie Intégrée des Cellules Rénales, Service de Biologie Cellulaire, CEA Saclay, Unité 1859 associée au CNRS, France.
Elalouf
J M
JM
Doucet
A
A
D000818:Animals; D004064:Digestive System / Q000201:enzymology; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D008297:Male; D009399:Nephrons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D017506:H(+)-K(+)-Exchanging ATPase