title: Velocity differences between L- and P-selectin-dependent neutrophil rolling in venules of mouse cremaster muscle in vivo.
abstract: After surgical trauma, leukocyte rolling is initially normal in L-selectin-deficient mice and reduced at later times, whereas leukocyte rolling is initially absent in P-selectin-deficient mice but induced later. Here, we examined the possibility that P- and L-selectin support rolling at different characteristic velocities using intravital microscopy of venules of the exteriorized cremaster muscle venules of wild type (WT) and P- and L-selectin-deficient mice. At > 50 min after exteriorization, rolling in P-selectin-deficient mice occurred at significantly higher velocities (129 +/- 89 microns/s) than in WT mice (49 +/- 23 microns/s). Rolling velocity distribution in L-selectin-deficient mice was similar to WT mice immediately after exteriorization. Histological examination of Giemsa-stained whole-mount preparations in cremaster muscle venules revealed that the majority of rolling cells (approximately 90% in all genotypes) were granulocytes. We conclude that P-selectin mediates leukocyte rolling at velocities < 50 microns/s, whereas L-selectin sustains more rapid rolling. Under physiological conditions, P- and L-selectin synergize to support rolling at velocities between 20 and 70 microns/s as seen in WT mice.
authors.affiliation: Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville 22908, USA.
authors.forename: U
authors.initials: U
authors.lastname: Jung
authors.forename: D C
authors.initials: DC
authors.lastname: Bullard
authors.forename: T F
authors.initials: TF
authors.lastname: Tedder
authors.forename: K
authors.initials: K
authors.lastname: Ley
mesh_terms: D000009:Abdominal Muscles / Q000098:blood supply; D000818:Animals; D002448:Cell Adhesion; D006824:Hybridization, Genetic; D019041:L-Selectin / Q000502:physiology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin / Q000502:physiology*; D013997:Time Factors; D014699:Venules / Q000502:physiology
chemical_list: D019007:P-Selectin; D019041:L-Selectin