Chitinase genes expressed by infective larvae of the filarial nematodes, Acanthocheilonema viteae and Onchocerca volvulus.
State-specific products of 220 and 75 kDa were identified by metabolic labelling of infective larvae of the filarial nematode Acanthocheilonema viteae in ticks. Synthesis was temperature sensitive, occurring at 27 degrees C but not at 37 degrees C. These products were secreted 3-6 days after leaving the vector during post-infective development, but subsequent expression was not detected. The smaller protein with a pI of 6.2, was purified by two-dimensional electrophoresis and the N-terminal amino acid sequence was derived. This provisionally identified the protein as a chitinase, which was confirmed biochemically by glycol-chitin substrate gel electrophoresis. The polymerase chain reaction was used to amplify a product from a cDNA library of A. viteae infective larvae. The nucleotide sequence codes for a putative signal peptide of 20 amino acids and a mature protein of 504 residues (Mr 56 kDa), exhibiting 69% identity (81% similarity allowing for conservative substitutions) with the MF1 chitinase described from microfilariae of Brugia malayi. N-linked glycosylation may account for some, or all, of the discrepancy in Mr between the predicted polypeptide and the native parasite product (75 kDa). Primers based on the A. viteae sequence were used to amplify a related sequence from a cDNA library of Onchocerca volvulus infective larvae. The O. volvulus cDNA codes for a 20-amino acid signal peptide followed by 477 residues with an Mr of 54 kDa, and shares 67% identity with the A. viteae chitinase (80% similarity allowing for conservative substitutions) and 69% identity with the B. malayi MF1 molecule. It is proposed that chitinases expressed by infective stages of these filarial nematodes may play a role in ecdysis during post-infective development.
Wu
Y
Y
Liverpool School of Tropical Medicine, UK.
Adam
R
R
Williams
S A
SA
Bianco
A E
AE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002688:Chitinases / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D004153:Dipetalonema / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D004790:Enzyme Induction; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000096:biosynthesis / Q000235:genetics*; D007814:Larva; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000469:parasitology
D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D002688:Chitinases