title: A new approach to labeling cells with technetium-99m, Part II. Evaluation of radiolabeled human-lymphocyte immune functions.
abstract: Technetium-99m-modified polylysine (MPL) is a mild and efficient method for cell labeling that is easily applicable to human lymphocytes. 99mTc-MPL uptake is maximal in 40 min at room temperature. Cell labeling efficiency (from 60 to 80%) increases with rising concentrations of cells and labeling agent. In vitro stability of 99mTc-MPL on lymphocytes incubated in serum at 37 degrees C is high. Microautoradiography indicates that 99mTc-MPL is equally distributed within the cells. The radiolabeling procedure does not alter expression of surface receptors involved in T lymphocyte effector functions, adhesion function, cytolytic activity or proliferative response to IL-2.
authors.affiliation: Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA USA.
authors.forename: L I
authors.initials: LI
authors.lastname: Delmon-Moingeon
authors.forename: A
authors.initials: A
authors.lastname: Mahmood
authors.forename: A
authors.initials: A
authors.lastname: Davison
authors.forename: A G
authors.initials: AG
authors.lastname: Jones
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology*; D015609:Organotechnetium Compounds; D011107:Polylysine; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D015609:Organotechnetium Compounds; C094942:technetium Tc 99m polylysine; D011107:Polylysine