title: Distribution and subcellular localization of a lysosome-associated protein in human genital organs.
abstract: The tissue distribution, preferentially in the human male genital system, and the subcellular localization of the lysosome-associated membrane protein 2 (lamp 2) was studied immunohistochemically using a mouse monoclonal antibody, 2D5. Strong immunoreactivity was present in the tubular system of the kidney, in acinar cells of salivary glands and pancreas, prostate, mammary glands, placenta and in cutaneous sweat glands. Moderate immunoreactivity was observed in cerebral neuronal cells, epidermal cells, testis, epididymis, seminal vesicle and endometrium. Very low immunoreactivity was found in liver. In some of the tissues mentioned, the distribution pattern of immunoreactivity is smooth and homogeneous, while in others it is granular and concentrated in the supra- or perinuclear cytoplasm. The subcellular distribution was studied on ultracryosections and on pre-embedding-processed chopper sections of human prostate. In the latter gland, the protein is not restricted to epithelium, but is also present in stromal cells. Ultrastructurally, the immunoreactivity in secretory cells was localized in electron-translucent vacuoles and granules, including the secretory granules. A close association with cell membranes was not generally the case. Only part of the immunoreactive material was linked to the apical plasma membrane pointing to a biosynthesis independent from an association step with the apical plasma membrane. As shown by immunoelectron microscopy and Western blotting, a high amount of lamp 2 is secreted and is found in so-called prostasomes. The findings indicate that in the human prostate most of the membrane-bound lamp 2 is released from the secretory cells, presumably in an apocrine fashion.
authors.affiliation: Department of Anatomy and Cell Biology, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033 Marburg an der Lahn, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Aumüller
authors.forename: H
authors.initials: H
authors.lastname: Renneberg
authors.forename: A
authors.initials: A
authors.lastname: Hasilik
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D001940:Breast / Q000737:chemistry; D016764:Cell Polarity; D003594:Cytoplasmic Granules / Q000737:chemistry; D004717:Endometrium / Q000737:chemistry; D005260:Female; D005837:Genitalia, Male / Q000737:chemistry*; D043425:Glutamate Carboxypeptidase II; D006801:Humans; D008099:Liver / Q000737:chemistry; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000737:chemistry*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D051379:Mice; D016253:Microscopy, Immunoelectron; D009928:Organ Specificity; D010920:Placenta / Q000737:chemistry; D011467:Prostate / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012469:Salivary Glands / Q000737:chemistry; D013347:Subcellular Fractions / Q000737:chemistry; D013545:Sweat Glands / Q000737:chemistry; D014617:Vacuoles / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II