NO synthase and guanylate cyclase inhibitors block modulation of the plasticity of common snail cholinoreceptors by 15-hydroxy-eicosatetraenoic acid.
The probable role of two second messengers, nitrogen oxide (NO) and cyclic guanosine monophosphate (cGMP) in the short- and long-latency effects of the acyclic eicosanoid 15(S)-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HETE) on the plasticity of somatic cholinoreceptors of identified RPa3 and LPa3 neurons of Helix lucorum, was investigated using the two-electrode voltage clamp technique on the membrane. It was demonstrated that N omega-methyl-L-arginine (an inhibitor of NO synthase), LY-83,583 [sic], and the dye methylene blue (inhibitors of soluble guanylate cyclase), when applied extracellularly, disrupt the short- and long-latency modulatory influences of 15-HETE on the depression of the inward current induced by acetylcholine during its rhythmic application to the soma. The participation of NO and cGMP in the modulatory effects of 15-HETE on the plasticity of cholinoreceptors is hypothesized.
Pivovarov
A S
AS
Department of Higher Nervous Activity, M. V. Lomonosov Moscow State University.
Egido-Villareal
W
W
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D006152:Cyclic GMP / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006372:Helix, Snails; D006893:Hydroxyeicosatetraenoic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D066298:In Vitro Techniques; D008751:Methylene Blue / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*; D018709:Statistics, Nonparametric; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D006893:Hydroxyeicosatetraenoic Acids; D011950:Receptors, Cholinergic; D019323:omega-N-Methylarginine; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D000109:Acetylcholine; D008751:Methylene Blue