Bradyrhizobium japonicum does not require alpha-ketoglutarate dehydrogenase for growth on succinate or malate.
The sucA gene, encoding the E1 component of alpha-ketoglutarate dehydrogenase, was cloned from Bradyrhizobium japonicum USDA110, and its nucleotide sequence was determined. The gene shows a codon usage bias typical of non-nif and non-fix genes from this bacterium, with 89.1% of the codons being G or C in the third position. A mutant strain of B. japonicum, LSG184, was constructed with the sucA gene interrupted by a kanamycin resistance marker. LSG184 is devoid of alpha-ketoglutarate dehydrogenase activity, indicating that there is only one copy of sucA in B. japonicum and that it is completely inactivated in the mutant. Batch culture experiments on minimal medium revealed that LSG184 grows well on a variety of carbon substrates, including arabinose, malate, succinate, beta-hydroxybutyrate, glycerol, formate, and galactose. The sucA mutant is not a succinate auxotroph but has a reduced ability to use glutamate as a carbon or nitrogen source and an increased sensitivity to growth inhibition by acetate, relative to the parental strain. Because LSG184 grows well on malate or succinate as its sole carbon source, we conclude that B. japonicum, unlike most other bacteria, does not require an intact tricarboxylic acid (TCA) cycle to meet its energy needs when growing on the four-carbon TCA cycle intermediates. Our data support the idea that B. japonicum has alternate energy-yielding pathways that could potentially compensate for inhibition of alpha-ketoglutarate dehydrogenase during symbiotic nitrogen fixation under oxygen-limiting conditions.
Green
L S
LS
Department of Biochemistry and Interdisciplinary Plant Group, University of Missouri, Columbia 65211, USA. bclgreen@muccmail.missouri.edu
Emerich
D W
DW
D000595:Amino Acid Sequence; D050260:Carbohydrate Metabolism; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D018698:Glutamic Acid / Q000378:metabolism; D007522:Isocitrate Lyase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics / Q000502:physiology*; D008293:Malates / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
D003062:Codon; D008293:Malates; D000644:Quaternary Ammonium Compounds; D013386:Succinates; D018698:Glutamic Acid; C030298:malic acid; D019802:Succinic Acid; D007655:Ketoglutarate Dehydrogenase Complex; D007522:Isocitrate Lyase