Ginsenosides increase secretion of lipoprotein lipase by 3T3-L1 adipocytes.
Treatment of 3T3-L1 adipocytes with either an oleanolic acid glycoside or a 20(S)-protopanaxatriol glycoside increased the secretion of lipoprotein lipase activity into the medium dose-dependently. At a concentration of 100 micrograms/ml, ginsenosides Ro, Re, Rg1, and Rh1 increased the secretion of lipase activity into the medium by 119, 107, 56, and 32%, respectively. The ratio of lipase activity in the medium to cellular lipase activity was 4.7% in control cells and 8.6% in ginsenoside Ro-treated cells, 8.3% in ginsenoside Re-treated cells, 7.0% in ginsenoside Rg1-treated cells, and 6.3% in ginsenoside Rh1-treated cells. Ginsenoside Rb2, which is a 20(S)-protopanaxadiol glycoside, increased the secretion of lipase activity by 16% at 25 micrograms/ml, and the ratio of lipase activity in the medium to cellular lipase activity was higher in ginsenoside Rb2-treated cells than in control cells. However, at 100 and 200 micrograms/ml, ginsenoside Rb2 decreased the secretion of lipase activity in parallel with cellular lipase activity. Ginsenoside Rd also decreased the secretion of lipase activity in the same dose-dependent manner. Thus, the effective dose for the secretion of lipoprotein lipase activity with ginsenosides varies with their aglycone structure.
Masuno
H
H
Department of Medical Laboratory Technology, Ehime College of Health Science, Japan.
Kitao
T
T
Okuda
H
H
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002491:Central Nervous System Agents / Q000737:chemistry / Q000494:pharmacology*; D003470:Culture Media; D036145:Ginsenosides; D008071:Lipoprotein Lipase / Q000378:metabolism*; D051379:Mice; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013329:Structure-Activity Relationship
D002491:Central Nervous System Agents; D003470:Culture Media; D036145:Ginsenosides; D012503:Saponins; D008071:Lipoprotein Lipase