title: DNA repair in microgravity: studies on bacteria and mammalian cells in the experiments REPAIR and KINETICS.
abstract: The impact of microgravity on cellular repair processes was tested in the space experiments REPAIR and KINETICS, which were performed during the IML-2 mission in the Biorack of ESA: (a) survival of spores of Bacillus subtilis HA101 after UV-irradiation (up to 340 J m-2) in the experiment REPAIR; (b) in the experiment KINETICS the kinetics of DNA repair in three different test systems: rejoining of X-ray-induced DNA strand breaks (B1) in cells of Escherichia coli B/r (120 Gy) and (B2) in human fibroblasts (5 and 10 Gy) as well as (B3) induction of the SOS response after gamma-irradiation (300 Gy) of cells of Escherichia coli PQ37. Cells were irradiated prior to the space mission and were kept in a non-metabolic state (metabolically inactive spores of B. subtilis on membrane filters, frozen cells of E. coli and human fibroblasts) until incubation in orbit. Germination and growth of B. subtilis were initiated by humidification, E. coli and fibroblasts were thawed up and incubated at 37 degrees C for defined repair periods (up to 4.5 h), thereafter they were frozen again for laboratory analysis. Relevant controls were performed in-flight (1 x g reference centrifuge) and on ground (1 x g and 1.4 x g) The results show no significant differences between the microgravity samples and the corresponding controls neither in the survival curves nor in the kinetics of DNA strand break rejoining and induction of the SOS response (proven by Student's t-test, 2 P = 0.05). These observations provide evidence that in the microgravity environment cells are able to repair radiation-induced DNA damage close to normality. The results suggest that a disturbance of cellular repair processes in the microgravity environment might not be the explanation for the reported synergism of radiation and microgravity.
authors.affiliation: DLR, Institute of Aerospace Medicine, Radiation Biology Division, Köln, Germany. gerda.horneck@dlr.de
authors.forename: G
authors.initials: G
authors.lastname: Horneck
authors.forename: P
authors.initials: P
authors.lastname: Rettberg
authors.forename: C
authors.initials: C
authors.lastname: Baumstark-Khan
authors.forename: H
authors.initials: H
authors.lastname: Rink
authors.forename: S
authors.initials: S
authors.lastname: Kozubek
authors.forename: M
authors.initials: M
authors.lastname: Schäfer
authors.forename: C
authors.initials: C
authors.lastname: Schmitz
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism / Q000528:radiation effects; D001709:Biotechnology; D002460:Cell Line; D004260:DNA Repair / Q000528:radiation effects; D004926:Escherichia coli / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D012107:Research Design; D013014:SOS Response, Genetics / Q000528:radiation effects; D013026:Space Flight; D013171:Spores, Bacterial / Q000378:metabolism / Q000528:radiation effects; D014893:Weightlessness / Q000009:adverse effects*