Scavenging effect of tetrandrine of active oxygen radicals.
The scavenging effects of tetrandrine (TD) on active oxygen radicals were investigated through chemiluminescence experiments and oxidation of ferrocytochrome c. The superoxide anions (O2-.) were generated either in hypoxanthine/xanthine oxidase (HX-XOD) system or by means of auto-oxidation of erythrocytes. It was found that TD (7.5 microM) could remarkably scavenge O2-. in the HX-XOD system and that the effect was concentration-dependent. The scavenging effect of TD on O2-. generated from auto-oxidation in erythrocytes was not as strong as in the HX-XOD system. The hydroxyl radicals (.OH) were generated in aqueous solution of H2O2 irradiated by UV, it was also found that TD (15 microM) effectively scavenged .OH in that system. This study led to the conclusion that the antioxidant property of TD may account for some of its pharmacological effects.
Cao
Z F
ZF
Department of Physiology, Hunan Medical University, Changsha, China.
D000328:Adult; D000470:Alkaloids / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D044182:Benzylisoquinolines; D003574:Cytochrome c Group / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism
D000470:Alkaloids; D000975:Antioxidants; D044182:Benzylisoquinolines; D003574:Cytochrome c Group; D016166:Free Radical Scavengers; D013481:Superoxides; C009438:tetrandrine