Isolation and promoter mapping of the gene encoding murine co-stimulatory factor B7-1.
B7-1 is one of the co-stimulatory factors which plays an important role in immunity. We have cloned and functionally mapped the promoter of the murine B7-1 gene using transient transfection assays in mouse L (tk-) cells. The B7-1 basal promoter consists of three positively regulated regions. The distal region, located at -2597 to -1555, contains an assortment of putative transcription factor binding sites. The proximal upstream region, located at -130 to -110, contains a tandem repeat sequence 5'-GTGTTCTAGTGTT-3'. A downstream region is positioned at +269 to +25. We have also identified an alternatively spliced form of the murine B7-1 gene in L (tk-) cells.
Zhang
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Department of Growth Factor Research, Abbott Laboratories, Abbott Park, IL 60064, USA. zhangh@randb.abbott.com
Haasch
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Idler
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Okasinski
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D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D005796:Genes / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D007739:L Cells; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D018122:B7-1 Antigen; D012333:RNA, Messenger; D014157:Transcription Factors; D008156:Luciferases