Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2.
Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite's genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum.
Camargo
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Zentrum für Infektionsforschung der Universität Würzburg, Germany.
Fischer
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K
Lanzer
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D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular / Q000379:methods; D018628:Gene Dosage; D017125:Genes, Protozoan; D015698:Genomic Library; D010963:Plasmodium falciparum / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere / Q000235:genetics