title: Separation and characterization of polyethylene wear debris from synovial fluid and tissue samples of revised knee replacements.
abstract: A study was made of in vivo-generated polyethylene wear particles as separated from synovial fluid samples and from tissue samples surrounding total knee arthroplasty. A comparison of particle size and morphology between the two particle groups was made to assess any effects of selective tissue capture, and macrophage encapsulation and digestion. In addition, a Raman spectroscopy technique was evaluated that enables positive identification of individual wear particles. The particles of the same size range found in the synovial fluid and tissue samples exhibited a comparable morphology. Notably, submicron-sized debris was present in both the synovial fluid and tissue samples surrounding knees with osteolysis. The novel micro-Raman analysis of individual particles was successful in the categorizing of wear debris as polyethylene or nonpolyethylene.
authors.affiliation: FDA Center for Devices and Radiological Health, Rockville, Maryland 20857, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Wolfarth
authors.forename: D W
authors.initials: DW
authors.lastname: Han
authors.forename: G
authors.initials: G
authors.lastname: Bushar
authors.forename: N L
authors.initials: NL
authors.lastname: Parks
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D007720:Knee Prosthesis; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010316:Particle Size; D011095:Polyethylenes / Q000737:chemistry / Q000302:isolation & purification*; D013059:Spectrum Analysis, Raman; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D011095:Polyethylenes