Role of stearoyl-CoA desaturase in the modification of acyl composition of hepatic phosphatidylcholine by peroxisome proliferators.
The relative contribution of stearoyl-CoA desaturase and 1-acylglycerophosphocholine (1-acyl-GPC) acyltransferase, which are induced by peroxisome proliferators, to the increase in the proportion of oleic acid in C-2 position of phosphatidylcholine (PtdCho) in the liver of rats was investigated. Rats were administered either 4-chlorophenoxyisobutyric acid (clofibric acid), 2,2'-(decamethylenedithio)-di-ethanol (tiadenol) or perfluorooctanoic acid in varying doses. With administration of these peroxisome proliferators, the proportion of oleic acid in C-2 position of PtdCho in hepatic microsomes was increased in commensurate with the increase in activities of stearoyl-CoA desaturase and 1-acyl-GPC acyltransferase. Multiple regression analysis revealed that although there is a high correlation among the three parameters examined, the partial correlation between the proportion of oleic acid in the C-2 position and the activity of stearoyl-CoA desaturase is significantly higher than that between the proportion of oleic acid in the C-2 position and the activity of 1-acyl-GPC acyltransferase. The contribution of stearoyl-CoA desaturase to the increase in the proportion of oleic acid in the C-2 position thus appears greater than that of 1-acyl-GPC acyltransferase.
Mizuguchi
H
H
Research Laboratories, Torii Pharmaceutical Co., Ltd., Chiba, Japan.
Kudo
N
N
Kawashima
Y
Y
D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002210:Caprylates / Q000494:pharmacology; D002995:Clofibric Acid / Q000737:chemistry / Q000494:pharmacology; D004032:Diet; D005233:Fatty Alcohols / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
D002210:Caprylates; D005233:Fatty Alcohols; D005466:Fluorocarbons; D000960:Hypolipidemic Agents; D009829:Oleic Acids; D010713:Phosphatidylcholines; C004568:tiadenol; D002995:Clofibric Acid; C023036:perfluorooctanoic acid; D013230:Stearoyl-CoA Desaturase; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase