Ex vivo astroglial-induced radial glia express in vivo markers.
Ex vivo induction of radial-like glia has been previously reported to occur following exposure of cerebral cortex subcultures from fetal origin to cerebral cortex astroglial-conditioned medium. The present report further confirms similarities between in vivo and ex vivo radial glia, using additional criteria: adhesion of primary cell dissociates to glial processes, with presumptive cell migration along them, punctuate labelling for laminin, and immunolabelling with Rat-401 antisera.
Colombo
J A
JA
Programa Unidad de Neurobiología Aplicada (PRUNA)(CEMIC-CONICET), Buenos Aires, Argentina.
Napp
M I
MI
D000818:Animals; D000918:Antibody Specificity; D001253:Astrocytes / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D015415:Biomarkers; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000276:immunology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014746:Vimentin / Q000032:analysis / Q000276:immunology
D015415:Biomarkers; D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D014746:Vimentin