Receptor affinity purification of a lipid-binding adhesin from Haemophilus influenzae.
Thirteen clinical strains of Haemophilus influenzae, including types b, d, and untypeable, in vitro specifically recognize phosphatidylethanolamine (PE), gangliotetraosylceramide, gangliotriosylceramide (Gg3), sulfatoxygalactosylceramide, and to a lesser extent sulfatoxygalactosylglycerol. A PE affinity matrix was used to purify an adhesin of approximately 46 kDa from both type b and untypeable H. influenzae. This adhesin was a potent inhibitor of H. influenzae Gg3 and PE binding in vitro, and polyclonal antibodies specific for this protein prevented the attachment of H. influenzae Gg3 and PE and cultured HEp-2 epithelial cells in vitro.
Busse
J
J
Department of Microbiology, Research Institute, Hospital for Sick Children, Toronto, Canada.
Hartmann
E
E
Lingwood
C A
CA
D018829:Adhesins, Bacterial / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D001422:Bacterial Adhesion; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D005732:Gangliosides; D006017:Glycolipids / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000145:classification / Q000502:physiology; D007106:Immune Sera; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018829:Adhesins, Bacterial; D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D007106:Immune Sera; D010714:Phosphatidylethanolamines; C038244:ganglio-N-triaosylceramide