The influence of some vegetable extracts on the in vitro adherence of mouse and human lymphocytes to nylon fibers.
We found that the total watery extracts obtained from roots of various plants such as Symphytum officinale, Phytolacca americana etc, precipitate human glycoproteins, agglutinate sheep red blood cells (SRBC) and stimulate lymphocyte adherence to nylon fibers. Five out of seven extracts precipitated human gammaglobulins and one of seven obviously agglutinate SRBC. If these cells were pretreated with rabbit antibodies against SRBC, all extracts agglutinated the cells at various degrees of intensity, the most active being Phytolacca americana. The adherence of mouse but not human lymphocytes to nylon fibers was stimulated by extracts of Symphytum officinale and Phytolacca americana. This process was neither stimulated nor inhibited by Mannose (Man), Galactose (Gal), Glucose (Glc), N-acethyl Galactose (GalNAc) and N-acethyl Glucose (Glc-NAc). These biological effects of the plant extracts could be the expression of a lectin-like ability to bind various sugars other than those mentioned. The results suggest the possibility of using different extracts as means to point out the presence in serum or at the cellular level of some carbohydrates influencing the cellular adhesion, phenomenon which plays an important role in the functions of hematopoietic cells.
Lenghel
V
V
CHRISTIANA Hospital, Bucharest, Romania.
Radu
D L
DL
Chirilă
P
P
Olinescu
A
A
D000371:Agglutination; D000818:Animals; D002448:Cell Adhesion; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000502:physiology; D005690:Galactose / Q000031:analogs & derivatives / Q000494:pharmacology; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000502:physiology; D008214:Lymphocytes / Q000378:metabolism*; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D009757:Nylons / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012756:Sheep
D007074:Immunoglobulin G; D009757:Nylons; D010936:Plant Extracts; D005947:Glucose; D008358:Mannose; D005690:Galactose