Thyroid hormone affects rat uterine expression of IGF-I and IGFBP-4.
The rat uterus has been shown to be a site of production of insulin-like growth factor-I (IGF-I) and multiple IGF-binding proteins (IGFBP-2, -3, -4, -5, -6) which are involved in estrogen-induced uterine proliferation. The presence of T3-receptors in rat uterus suggests a role of thyroid hormone in the regulation of uterus responses to estradiol. In this study IGF-I and IGFBP-4 mRNAs in uterus, oviduct and cervix from euthyroid, hypothyroid and T3-treated rats were quantified by Northern blot analysis. Our results demonstrate: i) a marked decrease in IGF-I and IGFBP-4 mRNA levels in the uterus but an increase in the oviduct of hypothyroid rats; ii) a marked increase in IGF-I and IGFBP-4 mRNA levels in the uterus but a net decrease in the cervix of T3-treated rats. The uterine changes in IGF-I and IGFBP-4 mRNA levels associated with hypothyroid status were in agreement with those observed in liver.
Bottazzi
C
C
Istituto di Fisiologia Generale, Unviersità di Genova.
Demori
I
I
Leone
M
M
Fugassa
E
E
D000818:Animals; D015152:Blotting, Northern; D002584:Cervix Uteri / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007037:Hypothyroidism / Q000378:metabolism*; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000008:administration & dosage / Q000097:blood; D014599:Uterus / Q000378:metabolism*
D018974:Insulin-Like Growth Factor Binding Protein 4; D012333:RNA, Messenger; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D013974:Thyroxine