Inflammation-seeking scintigraphy with radiolabeled biotinylated polyclonal IgG followed by the injection of avidin chase.
We tried to apply the avidin chasing system to the inflammation-seeking scintigraphy using radiolabeled nonspecific polyclonal IgG. We studied the pharmacokinetics of technetium-99m and iodine-125-labeled biotinylated murine polyclonal IgG followed by an avidin chase injection in model mice with inflammatory foci. Avidin chase decreased the circulating radioactivity of 99mTc and 125I, which was a major problem for inflammation-seeking scintigraphy using radiolabeled nonspecific polyclonal IgG, to 9.3% and 19.3% of that without an avidin chase injection, respectively. Inflammation-seeking scintigraphy with the aforementioned method would be better than that with conventional method.
Kobayashi
H
H
Department of Radiology and Nuclear Medicine, Kyoto University, Faculty of Medicine, Japan.
Sakahara
H
H
Endo
K
K
Yao
Z S
ZS
Konishi
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D001360:Avidin; D001710:Biotin / Q000493:pharmacokinetics; D018796:Immunoconjugates / Q000493:pharmacokinetics; D007074:Immunoglobulin G; D007249:Inflammation / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011786:Quality Control; D011877:Radionuclide Imaging; D013667:Technetium; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin; D013667:Technetium