Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7.
Nine Danish Actinobacillus pleuropneumoniae biotype 1 isolates were shown by latex agglutination and indirect haemagglutination to possess capsular polysaccharide epitopes identical to those of serotype 2 strain 1536 (reference strain of serotype 2) and strain 4226 (Danish serotype 2 strain). Immunodiffusion confirmed the antigenic relationship with serotype 2 and further demonstrated an antigenic relationship with strain WF83 (reference strain of serotype 7). SDS-PAGE with LPS from strains 1536, 4226, WF83 and strain 7317 (representative of the 9 isolates examined) showed that strains WF83 and 7317 had an identical smooth ladder pattern whereas LPS from strains 1536 and 4226 showed a distinctly different pattern. The antigenic similarities of the LPS of strains WF83 and 7317 were confirmed by immunoblots using rabbit or pig antisera prepared against the 3 strains. No antigenic similarities in the LPS of strains 1536 and 7317 were revealed. Since an antigenic determinant specific for the 9 isolates could not be demonstrated with the methods used, the strains are proposed to be designated K2:O7.
Nielsen
R
R
Danish Veterinary Laboratory, Copenhagen, Denmark.
Andresen
L O
LO
Plambeck
T
T
D000189:Actinobacillus Infections / Q000175:diagnosis*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D007106:Immune Sera; D015151:Immunoblotting; D007841:Latex Fixation Tests; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D013552:Swine
D007106:Immune Sera; D011135:Polysaccharides, Bacterial