title: Developmental and afterload stress regulation of heat shock proteins in the ovine myocardium.
abstract: Heat shock proteins (hsps) are produced in the myocardium in response to stresses such as ischemia, hyperthermia, and increased afterload. The role of these stress proteins in the developing myocardium is unknown. Expression of the inducible (hsp 72) and cognate (hsc 73) hsps was determined in the immature ovine myocardium during the perinatal transition, and their role in subsequent myocardial growth was examined. hsp synthesis was also studied during acute afterload stress in newborns by aortic banding to a gradient of 50 torr for 4 h. Expression of the inducible (hsp 72) isoform is developmentally regulated in both right and left ventricles: low levels in the fetus, increasing throughout development, and peaking in the 14-25-d newborn and adult. The cognate (hsc 73) isoform remains unchanged during development in the left ventricle but decreases with age in the right ventricle. The inducible (hsp 72) isoform is also developmentally regulated in the lung, increasing postnatally to a peak in the 14-25-d-old and adult sheep. Finally, newborn myocardium demonstrated a rapid increase in hsp expression in response to afterload stress, similar to that seen in the adult.
authors.affiliation: Department of Pediatrics, Stanford University, California 94305, USA.
authors.forename: E
authors.initials: E
authors.lastname: Strandness
authors.forename: D
authors.initials: D
authors.lastname: Bernstein
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006352:Heart Ventricles / Q000378:metabolism / Q000503:physiopathology; D009200:Myocardial Contraction / Q000502:physiology*; D017682:Myocardial Stunning / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism*; D011247:Pregnancy; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D013312:Stress, Physiological / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D016760:Proto-Oncogene Proteins c-fos