title: Redox effects on the excited-state lifetime in chlorosomes and bacteriochlorophyll c oligomers.
abstract: Oligomers of [E,E] BChl CF (8, 12-diethyl bacteriochlorophyll c esterified with farnesol (F)) and [Pr,E] BChl CF (analogously, M methyl, Pr propyl) in hexane and aqueous detergent or lipid micelles were studied by means of steady-state absorption, time-resolved fluorescence, and electron spin resonance spectroscopy. The maximum absorption wavelength, excited-state dynamics, and electron spin resonance (EPR) linewidths are similar to those of native and reconstituted chlorosomes of Chlorobium tepidum. The maximum absorption wavelength of oligomers of [E,E] BChl CF was consistently blue-shifted as compared to that of [Pr,E] BChl CF oligomers, which is ascribed to the formation of smaller oligomers with [E,E] BChl CF than [Pr,E] BChl CF. Time-resolved fluorescence measurements show an excited-state lifetime of 10 ps or less in nonreduced samples of native and reconstituted chlorosomes of Chlorobium tepidum. Under reduced conditions the excited-state lifetime increased to tens of picoseconds, and energy transfer to BChl a or long-wavelength absorbing BChl c was observed. Oligomers of [E,E] BChl CF and [Pr,E] BChl CF in aqueous detergent or lipid micelles show a similar short excited-state lifetime under nonreduced conditions and an increase up to several tens of picoseconds upon reduction. These results indicate rapid quenching of excitation energy in nonreduced samples of chlorosomes and aqueous BChl c oligomers. EPR spectroscopy shows that traces of oxidized BChl c radicals are present in nonreduced and absent in reduced samples of chlorosomes and BChl c oligomers. This suggests that the observed short excited-state lifetimes in nonreduced samples of chlorosomes and BChl c oligomers may be ascribed to excited-state quenching by BChl c radicals. The narrow EPR linewidth suggests that the BChl c are arranged in clusters of 16 and 6 molecules in chlorosomes of Chlorobium tepidum and Chloroflexus aurantiacus, respectively.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287-1604, USA.
authors.forename: P I
authors.initials: PI
authors.lastname: van Noort
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: R
authors.initials: R
authors.lastname: LoBrutto
authors.forename: R E
authors.initials: RE
authors.lastname: Blankenship
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D001429:Bacteriochlorophylls / Q000737:chemistry / Q000378:metabolism; D019414:Chlorobi / Q000378:metabolism*; D003902:Detergents; D004578:Electron Spin Resonance Spectroscopy; D006586:Hexanes; D007700:Kinetics; D046911:Macromolecular Substances; D008823:Micelles; D015388:Organelles / Q000378:metabolism*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D003902:Detergents; D006586:Hexanes; D046911:Macromolecular Substances; D008823:Micelles; C002084:bacteriochlorophyll c