Column-switching high-performance liquid chromatography combined with ionspray tandem mass spectrometry for the simultaneous determination of the platelet inhibitor Ro 44-3888 and its pro-drug and precursor metabolite in plasma.
A liquid chromatographic/mass spectrometric (LC/MS) assay was developed for the simultaneous determination of a pro-drug (Ro 48-3657), its active metabolite (platelet inhibitor, Ro 44-3888) and precursor metabolite (Ro 48-3656) in human, dog and rat plasma, utilizing on-line column-switching solid-phase extraction (SPE) for clean-up and high-performance liquid chromatography (HPLC) for separation of the analytes, with on-line detection by ionspray (pneumatically assisted electrospray) tandem mass spectrometry in the selected reaction monitoring (SRM) mode. The assay was validated for the quantification of all three analytes. The method involves protein precipitation with perchloric acid, enrichment of the analytes on a standard bore trapping column (i.d. 4.6 mm) and separation on a narrow-bore analytical column (i.d. 2 mm). Except for the plasma precipitation step, the assay was fully automated, allowing unattended operation. The lower limits of quantification were 0.20 ng ml-1 (Ro 48-3657, Ro 44-3888) and 0.50 ng ml-1 (Ro 48-3656) using a 0.5 ml plasma aliquot. The mean inter-assay precision and accuracy derived from quality control samples were 5.3% and 101%, respectively, utilizing the calibration range 0.2-200 ng ml-1. Using the unique features of column-switching HPLC combined with MS/MS, it was possible to develop the method in a short period of time. The method has been successfully applied to map complete concentration-time courses for the kinetic evaluation of the drug and its metabolites in man, dog and rat. This LC/MS assay is sensitive, specific, accurate, precise and robust.
Zell
M
M
Department of Drug Metabolism and Kinetics, F. Hoffmann-La Roche Ltd., Basle, Switzerland.
Husser
C
C
Hopfgartner
G
G
D000578:Amidines / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004285:Dogs; D006801:Humans; D013058:Mass Spectrometry; D015394:Molecular Structure; D010091:Oximes / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D010880:Piperidines / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors; D011355:Prodrugs / Q000032:analysis / Q000737:chemistry; D051381:Rats; D012015:Reference Standards
D000578:Amidines; D010091:Oximes; D010880:Piperidines; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011355:Prodrugs; C100916:Ro 44-3888; C100917:sibrafiban