title: Extraction and characterization of the smooth-type lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and its biological activities.
abstract: The lipopolysaccharide (LPS) of Fusobacterium nucleatum JCM 8532 was isolated by hot-phenol water extraction. Most of the LPS was extracted in the phenolic phase and shown to be the smooth-type, whereas the aqueous phase contained mainly rough-type LPS. The chemical composition of the LPS was similar to that reported in other studies, but D-quinovosamine, which may be a major component of O-antigenic polysaccharide, and 3-deoxy-D-manno-2-octulosonic acid (Kdoi) were detected for the first time by gas chromatography-mass spectrometry. The biological activities of smooth-type LPS, including limulus activity, lethal toxicity, pyrogenicity, and B lymphocyte mitogenicity, were comparable to those of entero-bacterial LPS. Smooth-type LPS inhibited the cell growth and DNA synthesis of adult and fetal human gingival fibroblasts in a dose-dependent manner, suggesting that LPS may play a role in the occurrence of human gingivitis.
authors.affiliation: Department of Preventive and Community Dentistry, Nippon Dental University, School of Dentistry at Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Onoue
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: Y
authors.initials: Y
authors.lastname: Isshiki
authors.forename: K
authors.initials: K
authors.lastname: Kawahara
mesh_terms: D000328:Adult; D002241:Carbohydrates / Q000032:analysis; D005347:Fibroblasts / Q000473:pathology; D016967:Fusobacterium nucleatum / Q000737:chemistry*; D005891:Gingivitis / Q000209:etiology; D005944:Glucosamine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D019081:O Antigens / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D013400:Sugar Acids / Q000032:analysis
chemical_list: D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D005944:Glucosamine; C009149:6-desoxy-D-glucosamine