title: Isolation and characterization of a bacterial growth-stimulating peptide from a peptic bovine hemoglobin hydrolysate.
abstract: A peptide with a bacterial-growth-stimulating activity was isolated from a bovine hemoglobin hydrolysate by reversed-phase high-performance liquid chromatography. Its primary structure and molecular mass, determined by amino acid analysis and fast-atom bombardment mass spectrometry, were identical to those of fragment 48-52 (Ser-Thr-Ala-Asp-Ala) of the beta chain of bovine hemoglobin. The microbiological tests in solid media demonstrated that this peptide exhibited a growth-stimulating activity on gram-negative bacteria.
authors.affiliation: Laboratoire de Génie Protéique et Cellulaire, Université de La Rochelle, France.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Zhao
authors.forename: J M
authors.initials: JM
authors.lastname: Piot
authors.forename: V
authors.initials: V
authors.lastname: Gautier
authors.forename: G
authors.initials: G
authors.lastname: Cottenceau
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D006094:Gram-Positive Bacteria / Q000254:growth & development*; D006133:Growth Substances / Q000737:chemistry* / Q000302:isolation & purification*; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D006133:Growth Substances; D006454:Hemoglobins; D010455:Peptides