title: Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.
abstract: Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed efficient assembly of infectious virus, whereas the reciprocal SIN/SFV(spike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.
authors.affiliation: Department of Biosciences at Novum, Huddinge, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Smyth
authors.forename: M
authors.initials: M
authors.lastname: Suomalainen
authors.forename: H
authors.initials: H
authors.lastname: Garoff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D012672:Semliki forest virus / Q000235:genetics / Q000502:physiology*; D012845:Sindbis Virus / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000096:biosynthesis; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins