The effect of iodine-based contrast agents on the levels of radiation-induced chromosomal aberrations.
The effects of iodine-based contrast agents on the repair of radiation-induced chromosomal damage were investigated employing peripheral blood from a healthy male donor. The blood samples were irradiated with 0.5-4.0 Gy 137Cs gamma rays. Contrast agents and NaCl solutions of various concentrations were added to the blood within the first 15 min or at 60 min after irradiation, and the samples were subsequently cultured for 45 h at 37 degrees C. Significantly elevated frequencies of chromosomal abnormalities caused by postirradiation treatment with hypertonic contrast agents appeared to increase with increasing hypertonicity. Elevated aberration frequencies were found to be greatest in the samples treated within 15 min of irradiation. The contrast agents had little effect if they were added at 60 min after irradiation, probably because the process of chromosome rejoining had been completed. Isotonic iodine-based contrast agents did not enhance the frequencies of chromosomal aberrations to a significant degree.
Matsubara
S
S
Department of Radiology, Yokohama City University, School of Medicine, Kanazawa-ku, Japan.
Kong
Z S
ZS
Omura
M
M
Kurihara
H
H
Torigoe
S
S
Iwasawa
T
T
Yoshida
T
T
Kubota
N
N
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000187:drug effects / Q000528:radiation effects*; D003287:Contrast Media / Q000494:pharmacology* / Q000633:toxicity; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D003973:Diatrizoate / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology* / Q000633:toxicity; D007479:Iopamidol / Q000494:pharmacology* / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D011836:Radiation Tolerance / Q000187:drug effects*; D013997:Time Factors; D014283:Triiodobenzoic Acids / Q000494:pharmacology* / Q000633:toxicity
D003287:Contrast Media; D006982:Hypertonic Solutions; D014283:Triiodobenzoic Acids; D003973:Diatrizoate; C033300:iotrolan; D007479:Iopamidol