Herpes simplex virus antibodies in the perilymph of patients with Menière disease.
OBJECTIVE
To evaluate the presence of IgG antibodies directed to herpes simplex virus (HSV) in the perilymph of patients with Menière disease.


METHODS
Antibodies to HSV, Epstein-Barr virus, cytomegalovirus, and measles virus were analyzed in serum and perilymph samples by enzyme-linked immunosorbent assay. Total IgG and albumin in serum and perilymph samples were measured by nephelometer analysis. The relation of specific antivirus IgG in the perilymph vs the serum was expressed as an index.


METHODS
Perilymph and serum samples from 7 patients with long-standing, disabling Menière disease were collected during therapeutic vestibulotomy. Perilymph and serum samples from 7 patients with otosclerosis and 2 recipients of cochlear implants were used as controls.


RESULTS
Compared with the corresponding serum sample, the perilymph from the patients with Menière disease disclosed a higher level of specific anti-HSV IgG. An elevated level of specific anti-measles virus IgG in the perilymph was detected in patients with otosclerosis. Patients of all groups showed no variation of specific anti-Epstein Barr virus IgG and anti-cytomegalovirus IgG in the serum or in the perilymph.


CONCLUSIONS
Our results show the presence of HSV IgG in the perilymph of patients with Menière disease and support the hypothesis that HSV may play an important role in the etiopathogenesis of Menière disease.
Arnold
W
W
Department of Otolaryngology, Head and Neck Surgery, Klinikum rechts der Isar, Technical University, Munich, Germany.
Niedermeyer
H P
HP
D000914:Antibodies, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D008575:Meniere Disease / Q000276:immunology / Q000821:virology*; D008875:Middle Aged; D010498:Perilymph / Q000821:virology; D018139:Simplexvirus / Q000302:isolation & purification*
D000914:Antibodies, Viral; D007074:Immunoglobulin G