title: Contrasting effects of mitogenic growth factors on oligodendrocyte precursor cell migration.
abstract: We have examined the effects of the mitogenic growth factors platelet derived growth factor (PDGF), basic fibroblast growth factor (bFGF) and glial growth factor-2 (GGF-2) on oligodendrocyte precursor migration. In an agarose drop migration assay PDGF and bFGF stimulated migration while GGF-2 had no effect. The migration-enhancing effect of bFGF cannot be blocked by neutralising antibodies against PDGF, confirming that this effect is direct and not mediated via upregulation of PDGF receptors. Based on our results, we propose a model in which the differing effects of PDGF and GGF-2 ensure appropriate numbers of oligodendrocyte precursor cells in the vicinity of axons to be myelinated during development.
authors.affiliation: Wellcome/CRC Institute of Developmental Biology and Cancer, Cambridge, England.
authors.forename: R
authors.initials: R
authors.lastname: Milner
authors.forename: H J
authors.initials: HJ
authors.lastname: Anderson
authors.forename: R F
authors.initials: RF
authors.lastname: Rippon
authors.forename: J S
authors.initials: JS
authors.lastname: McKay
authors.forename: R J
authors.initials: RJ
authors.lastname: Franklin
authors.forename: M A
authors.initials: MA
authors.lastname: Marchionni
authors.forename: R
authors.initials: R
authors.lastname: Reynolds
authors.forename: C
authors.initials: C
authors.lastname: Ffrench-Constant
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000166:cytology / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D020904:Glia Maturation Factor; D006023:Glycoproteins / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D020880:Neuregulins; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012685:Sepharose; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D020904:Glia Maturation Factor; D006023:Glycoproteins; D006131:Growth Inhibitors; D008934:Mitogens; D009419:Nerve Tissue Proteins; D020880:Neuregulins; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D012685:Sepharose