Characterization of the interactions of type XII collagen with two small proteoglycans from fetal bovine tendon, decorin and fibromodulin.
In addition to the major collagens, such as type I or type II, connective tissues contain a number of less abundant collagens and proteoglycans, whose association contributes to the different properties of the tissues. Type XII and type XIV collagens have been described in soft connective tissues, and type XIV collagen has been shown to interact specifically with decorin through its glycosaminoglycan chain (Font et al., J. Biol. Chem. 268, 25015-25018, 1993). Interactions between these collagens and the small proteoglycans have been characterized further by studying the binding of type XII collagen to decorin by solid phase assays. Our results show a saturable binding of the proteoglycan through its glycosaminoglycan chain to type XII collagen, which does not seem to involve the large non-collagenous NC3 domain of the molecule. This interaction is strongly inhibited by heparin. Furthermore, we report that another small proteoglycan, fibromodulin, isolated from tendon under non-denaturing conditions, is able to bind to type XII collagen. This interaction has been characterized and, unlike that observed with decorin, type XII collagen-fibromodulin interaction seems to take place with the core protein of the proteoglycan. In addition, we report that type XII-type I collagen interactions are not necessarily mediated by decorin as previously suggested.
Font
B
B
Institut de Biologie et Chimie des Proteines, CNRS, Lyon, France.
Eichenberger
D
D
Rosenberg
L M
LM
van der Rest
M
M
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000378:metabolism*; D058575:Decorin; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D005333:Fetus; D000071235:Fibromodulin; D006493:Heparin / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D013710:Tendons / Q000378:metabolism*
D002352:Carrier Proteins; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D000071235:Fibromodulin; D006493:Heparin; D003094:Collagen