Highly repetitive sequences and characteristics of genomic DNA in unicellular cyanobacterial strains.
Microcystis aeruginosa (Synechocystis) is a unicellular cyanobacterium that performs oxygenic photosynthesis. We found two novel sets of repetitive sequences, A (REP-A) and B (REP-B), on the M. aeruginosa K-81 genomic DNA, which consisted of distinct motifs of tandem repeated sequences located in the up- and downstream regions of the orf1 structural gene, respectively. Genomic Southern hybridization revealed multicopies of REP-A and -B on the genome. Furthermore, genomic Southern blots of cyanobacteria species with the REP-A and -B probes revealed that different hybridization signals appeared on the genomic DNAs of all 12 Microcystis strains, but no signal appeared on those of Synechocystis sp. PCC 6803, Synechococcus sp. PCC 7942, and Anabaena sp. PCC 7120.
Asayama
M
M
Division of Biotechnology, School of Agriculture, Ibaraki University, Japan.
Kabasawa
M
M
Takahashi
I
I
Aida
T
T
Shirai
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D000458:Cyanobacteria / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004269:DNA, Bacterial