Presence of F420-dependent glucose-6-phosphate dehydrogenase in Mycobacterium and Nocardia species, but absence from Streptomyces and Corynebacterium species and methanogenic Archaea.
A range of organisms known to contain F420 or to be relatives of mycobacteria were examined for F420-dependent glucose-6-phosphate dehydrogenase (FGD) and NADP-dependent glucose-6-phosphate dehydrogenase (NADP-G6PD) activities. All free-growing Mycobacterium species examined (including a virulent Mycobacterium tuberculosis strain) had FGD activities of 0.014-0.418 mumol min-1 mg protein-1, and NADP-G6PD activities of 0.013-0.636 mumol min-1 mg-1. Armadillo-grown Mycobacterium leprae had FGD activity of 0.008 mumol min-1 mg-1, but no detectable NADP-G6PD activity. Nocardia species also had FGD activity (0.088-0.154 mumol min-1 mg-1). Streptomyces and Corynebacterium species had no FGD, but had NADP-G6PD. Methanogenic Archaea had neither activity.
Purwantini
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E
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
Gillis
T P
TP
Daniels
L
L
D003352:Corynebacterium / Q000201:enzymology; D019605:Euryarchaeota / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D009161:Mycobacterium / Q000201:enzymology*; D009166:Mycobacterium leprae / Q000201:enzymology; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009615:Nocardia / Q000201:enzymology*; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology
D009243:NAD; D009249:NADP; C015084:coenzyme F420; D005954:Glucosephosphate Dehydrogenase; D012256:Riboflavin