title: DNA in situ hybridization for the rapid diagnosis of massive necrotizing avian adenovirus hepatitis and pancreatitis in chicks.
abstract: The present study describes the use of DNA in situ hybridization for the rapid diagnosis of massive necrotizing adenovirus hepatitis and pancreatitis in broiler chicks. A light microscope and DNA probes were used to identify avian adenovirus in replicate sections of formalin-fixed paraffin-embedded liver and pancreas from field and experimental chicks. Avian adenovirus infection was confirmed by transmission electron microscopy and virus isolation.
authors.affiliation: Georgia Poultry Laboratory, Oakwood 30566, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Goodwin
authors.forename: K S
authors.initials: KS
authors.lastname: Latimer
authors.forename: R S
authors.initials: RS
authors.lastname: Resurreccion
authors.forename: P G
authors.initials: PG
authors.lastname: Miller
authors.forename: R P
authors.initials: RP
authors.lastname: Campagnoli
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000175:diagnosis*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D008099:Liver / Q000737:chemistry / Q000473:pathology / Q000821:virology; D008854:Microscopy, Electron / Q000379:methods / Q000662:veterinary; D010179:Pancreas / Q000737:chemistry / Q000473:pathology / Q000821:virology; D010195:Pancreatitis / Q000175:diagnosis / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis*
chemical_list: D004279:DNA, Viral