title: Influence of peppermint oil on absorptive and secretory processes in rat small intestine.
abstract: BACKGROUND
  Peppermint oil is used to relieve the symptoms of irritable bowel syndrome, relaxing intestinal smooth muscle by reducing the availability of calcium, but its effects on intestinal transport are unknown.
  
  
  OBJECTIVE
  To determine the effect of peppermint oil on intestinal transport processes.
  
  
  METHODS
  The influence of peppermint oil on intestinal transport was investigated in rat jejunum using both intestinal sheets mounted in Ussing chambers and brush border membrane vesicles.
  
  
  RESULTS
  Mucosal peppermint oil (1 and 5 mg/ml) had no significant effect on basal short circuit current, but inhibited the increase associated with sodium dependent glucose absorption. The increased short circuit current induced by serosal acetylcholine, a reflection of calcium mediated electrogenic chloride secretion, was unaffected by mucosal peppermint oil (5 mg/ml). In contrast, serosal peppermint oil (1 mg/ml) inhibited the response to acetylcholine without reducing the effect of mucosal glucose. In brush border membrane vesicles active glucose uptake was inhibited by extravesicular peppermint oil at concentrations of 0.5 and 1 mg/ml.
  
  
  CONCLUSIONS
  Peppermint oil in the intestinal lumen inhibits enterocyte glucose uptake via a direct action at the brush border membrane. Inhibition of secretion by serosal peppermint oil is consistent with a reduced availability of calcium.
authors.affiliation: Department of Biomedical Science, Sheffield University.
authors.forename: A
authors.initials: A
authors.lastname: Beesley
authors.forename: J
authors.initials: J
authors.lastname: Hardcastle
authors.forename: P T
authors.initials: PT
authors.lastname: Hardcastle
authors.forename: C J
authors.initials: CJ
authors.lastname: Taylor
mesh_terms: D000042:Absorption; D000818:Animals; D005947:Glucose / Q000032:analysis / Q000493:pharmacokinetics*; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D036142:Mentha piperita; D010276:Parasympatholytics / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010276:Parasympatholytics; D010938:Plant Oils; C015424:peppermint oil; D005947:Glucose