title: Oxytocin receptors in bovine cervix during pregnancy and parturition: gene expression and cellular localization.
abstract: OBJECTIVE
  Our purpose was to determine the expression of transcripts encoding the oxytocin receptor protein in bovine cervix during pregnancy and parturition, the cellular localization of immunoreactive oxytocin receptors, and oxytocin receptors concentrations in the same tissues.
  
  
  METHODS
  Ribonuclease protection assay for oxytocin receptor messenger ribonucleic acid was used to determine gene expression in bovine cervical tissues obtained from 20 cows throughout pregnancy and parturition, cellular localization of oxytocin receptors was determined by immunohistochemistry, and tritiated oxytocin binding was measured in each tissue.
  
  
  RESULTS
  Oxytocin receptor gene expression and tritiated oxytocin binding were well correlated in each instance. During pregnancy the level of oxytocin receptor messenger ribonucleic acid was very low; it was increased at term with a further, marked increase at parturition. Tritiated oxytocin binding also increased dramatically at parturition and was most abundant in the mucosal layer. Strong oxytocin receptor immunoreactivity was present in mucosal epithelial cells, and scattered muscle cells in the muscular part showed the signal.
  
  
  CONCLUSIONS
  Our results, together with the previous finding that oxytocin stimulates prostaglandin E2 release from cervical tissue in vitro, indicate that cervical mucosal epithelial cells are targets for oxytocin at parturition and may mediate release of prostaglandin E2.
authors.affiliation: Department of Obstetrics and Gynecology, Cornell University Medical College, New York, NY 10021, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Fuchs
authors.forename: R
authors.initials: R
authors.lastname: Ivell
authors.forename: M
authors.initials: M
authors.lastname: Balvers
authors.forename: S M
authors.initials: SM
authors.lastname: Chang
authors.forename: M J
authors.initials: MJ
authors.lastname: Fields
mesh_terms: D000818:Animals; D002417:Cattle; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007743:Labor, Obstetric / Q000378:metabolism*; D009994:Osmolar Concentration; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018045:Receptors, Oxytocin / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D018045:Receptors, Oxytocin