title: cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen.
abstract: A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature processed protein of 102 aa with a molecular mass of 11344 Da. The expressed recombinant protein, named rPar j 2.0101, was a major allergen since it reacted with IgE of 82% (23/28) of the sera of Pj-allergic subjects analyzed. It was shown to be a new allergen since (i) the amino acid sequence homology with the already reported recombinant allergen Par j 1.0101 was 45% and (ii) there was no cross-inhibition between rPar j 2.0101 and rPar j 1.0101. In addition, rPar j 2.0101 inhibited 35% of the specific IgE for 10-14 kDa native allergens and preincubation of sera from Pj-allergic patients with both rPar j 2.0101 and rPar j 1.0101 fully abolished the IgE recognition of the 10-14 kDa native allergen region, suggesting that these two allergens contributed to the region.
authors.affiliation: Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Duro
authors.forename: P
authors.initials: P
authors.lastname: Colombo
authors.forename: M A
authors.initials: MA
authors.lastname: Costa
authors.forename: V
authors.initials: V
authors.lastname: Izzo
authors.forename: R
authors.initials: R
authors.lastname: Porcasi
authors.forename: R
authors.initials: R
authors.lastname: Di Fiore
authors.forename: G
authors.initials: G
authors.lastname: Locorotondo
authors.forename: M G
authors.initials: MG
authors.lastname: Mirisola
authors.forename: R
authors.initials: R
authors.lastname: Cocchiara
authors.forename: D
authors.initials: D
authors.lastname: Geraci
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D018076:DNA, Complementary; C103299:Par j 2.0101; D010940:Plant Proteins; D021382:Protein Sorting Signals