Fluorophotometry with a small animal adapter.
The usefulness of the Fluorotron Master fluorophotometer fitted with a small animal adapter was evaluated with tree shrews. We also determined that the optimal measurement parameters for these animals, which are regarded as promising for experimental use in ophthalmic research, are: fluorescein-Na concentration, 0.5 x 10(-9) to 1.0 x 10(-6) g/mL; fluorescein-Na dosage, 2 mg/kg; measurement time, 30 minutes after injection of fluorescein-Na into the ocular compartments. Results indicated that ultrafiltration for the measurement of protein-unbound fluorescein can be done with a hematocrit tube and a minimal blood sample, thereby reducing the impact of the sampling on the animal's general condition. The Fluorotron Master with the small animal adapter can offer advantages in estimating the blood-ocular barrier permeability using disease models in tree shrews.
Ishiko
S
S
Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan.
Yoshida
A
A
Kitaya
N
N
Abiko
T
T
Mori
F
F
Kado
M
M
Takahashi
M
M
Saito
K
K
D000818:Animals; D001813:Blood-Retinal Barrier / Q000502:physiology; D002199:Capillary Permeability; D019793:Fluorescein; D005452:Fluoresceins / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D015590:Fluorophotometry / Q000295:instrumentation / Q000379:methods*; D007275:Injections, Intravenous; D014418:Tupaiidae
D005452:Fluoresceins; D005456:Fluorescent Dyes; D019793:Fluorescein