Electrophoretic analysis of mammalian hair keratins.
Keratins extracted from the hair of several mammals were characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). By SDS-PAGE, most differences between species were detected in the position and intensity of polypeptide bands with MW in the range 30-10 KDa (high sulphur keratins, hair matrix proteins, HMP). By IEF, differences were found in the intensity of polypeptide bands in the acidic pH range (< 5.1). Electrophoretic techniques could therefore have a taxonomic usefulness.
Folin
M
M
Department of Biology, University of Padova, Italy.
Contiero
E
E
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007525:Isoelectric Focusing; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D013045:Species Specificity
D007633:Keratins