Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.
Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed efficient assembly of infectious virus, whereas the reciprocal SIN/SFV(spike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.
Smyth
J
J
Department of Biosciences at Novum, Huddinge, Sweden.
Suomalainen
M
M
Garoff
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D012672:Semliki forest virus / Q000235:genetics / Q000502:physiology*; D012845:Sindbis Virus / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000096:biosynthesis; D019065:Virus Assembly; D014779:Virus Replication
D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins