A cDNA encoding a putative 37 kDa leucine-rich repeat (LRR) protein, p37NB, isolated from S-type neuroblastoma cell has a differential tissue distribution.
In human neuroblastoma cells in culture, three morphologically distinct types of cells are observed: neuroblastic N-type cells, Schwannian S-type cells, and intermediate I-type cells. To investigate the differences in gene expression between N-type LA1-55N and S-type LA1-5S cells of the human neuroblastoma cell line LA-N-1, we constructed a subtractive cDNA library from LA1-5S cells. One of the genes that are expressed more in S-type cells than in N-type cells was identified as previously undescribed and is the focus of this report. We cloned a full-length cDNA of this gene, p37NB, and determined its sequence. A homology search against the GenBank database showed that this was from a novel gene encoding a putative 37 kDa leucine-rich repeat (LRR) protein. Northern blot hybridization and RT-PCR showed that the p37NB gene was differentially expressed in S-type compared to N-type cells of a few neuroblastoma cell lines.
Kim
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D
Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
LaQuaglia
M P
MP
Yang
S Y
SY
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007930:Leucine / Q000235:genetics*; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000737:chemistry* / Q000145:classification; D011506:Proteins / Q000737:chemistry*; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011506:Proteins; D007930:Leucine