title: Regulation of neuronal diversity in the Xenopus retina by Delta signalling.
abstract: To generate the variety of mature neurons and glia found in the developing retina, the competence of pluripotent progenitor cells to respond to extracellular signals must be controlled. Delta, a ligand of the Notch receptor, is a candidate for regulating progenitor competence on the grounds that activation of the pathway involving Notch and Delta can inhibit cellular differentiation. Here we test this possibility in the developing Xenopus retina by misexpression of Delta messenger RNA. We find that Delta-misexpressing cells with wild-type neighbours adopt earlier fates, primarily becoming ganglion cells and cone photoreceptors. Progenitors transfected with Delta later in development also produce rod photoreceptors, but not the latest-generated cell types, demonstrating the importance of timing in Delta function. We conclude that Delta signalling in the vertebrate retina is a basic regulatory mechanism that can be used to generate neuronal diversity.
authors.affiliation: Department of Biology, University of California, San Diego, California 92093, USA.
authors.forename: R I
authors.initials: RI
authors.lastname: Dorsky
authors.forename: W S
authors.initials: WS
authors.lastname: Chang
authors.forename: D H
authors.initials: DH
authors.lastname: Rapaport
authors.forename: W A
authors.initials: WA
authors.lastname: Harris
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002999:Clone Cells; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000378:metabolism; D051880:Receptors, Notch; D012160:Retina / Q000166:cytology* / Q000196:embryology; D015398:Signal Transduction; D013234:Stem Cells / Q000502:physiology; D014162:Transfection; D014981:Xenopus
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; C064045:delta protein