title: Cloning, expression, and mapping of CKAPI, which encodes a putative cytoskeleton-associated protein containing a CAP-GLY domain.
abstract: From a human fetal-brain cDNA library we isolated a novel cDNA clone encoding a protein containing a CAP-GLY domain that is highly conserved among several cytoskeleton-associated proteins. The CAP-GLY domain is thought to be essential for their association with microtubules. The cDNA, designated CKAPI (for cytoskeleton-associated protein I, glycine motif) contained an open reading frame of 579 nucleotides encoding 193 amino acids. Northern-blot analysis revealed expression of three transcripts, 1.0, 3.4, and 4.6 kb in size, in all tissues examined. The 1.0-kb transcript was significantly higher in brain and heart than in other tissues. This gene was mapped by FISH to chromosome bands 19q13.11-->q13.12.
authors.affiliation: Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: T K
authors.initials: TK
authors.lastname: Watanabe
authors.forename: F
authors.initials: F
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Nagata
authors.forename: A
authors.initials: A
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D003598:Cytoskeletal Proteins