title: Segregation analysis of the testis-determining autosomal trait, Tda, that differs between the C57Bl/6J and DBA/2J mouse strains suggests a multigenic threshold model.
abstract: The testis-determining autosomal trait (Tda) of the mouse was uncovered when the Y chromosome of the poschiavinus variety of Mus musculus domesticus was introduced into the C57BL/6J laboratory strain background. Testis development is normal in the F1 generation but, in the backcross and subsequent crosses to C57BL/6J females, XY individuals with the poschiavinus Y chromosome expressed bilateral ovaries or various combinations of an ovotestis with a contralateral ovary or testis or bilateral ovotestes and few had testes bilaterally. In other strain backgrounds, such as DBA/2J, XY individuals with the poschiavinus Y chromosome always expressed normal testes bilaterally. The first breeding analysis of this difference in the interaction of strain background with the poschiavinus Y chromosome suggested that the Tda trait was due to a single gene, but attempts to map it failed. We constructed two strains of C57BL/6J and DBA/2J that are consomic for the poschiavinus Y chromosome in order to conduct a segregation analysis of the Tda trait. In the C57BL/6J.Y-POS consomic strain, liability to express incomplete testis development is normally distributed and thresholds in development specify the probability of different classes of ovary, ovotestis, and testis combinations. Testis development is complete in the DBA/2J.Y-POS consomic strain. We demonstrated previously that the Tda trait of C57BL/6J is recessive to that of DBA/2J and the segregating first backcross generation of embryos rejected the single-gene model. We have extended our analysis to a F2 generation of embryos that also rejects a single-gene model. We also report a test mating analysis of the first backcross generation. It was initiated to provide an independent assessment of the single-gene model, but the analysis of the distribution of test mating results suggests that the difference in the Tda trait between C57BL/6J and DBA/2J may be due to a small number of loci, possibly four or five, and that the phenotypic effect between loci may be additive.
authors.affiliation: Department of Medical Genetics, University of Calgary, Canada.
authors.forename: J R
authors.initials: JR
authors.lastname: Eisner
authors.forename: B A
authors.initials: BA
authors.lastname: Eales
authors.forename: F G
authors.initials: FG
authors.lastname: Biddle
mesh_terms: D000818:Animals; D001699:Biometry; D003433:Crosses, Genetic; D012734:Disorders of Sex Development / Q000235:genetics; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology* / Q000235:genetics*; D008811:Mice, Inbred DBA / Q000196:embryology* / Q000235:genetics*; D008957:Models, Genetic; D005810:Multigene Family; D010641:Phenotype; D011247:Pregnancy; D012733:Sex Differentiation / Q000235:genetics*; D013045:Species Specificity; D013737:Testis / Q000196:embryology*; D014998:Y Chromosome / Q000235:genetics