title: Structure and analysis of phospholipase D gene from Ricinus communis L.
abstract: Phospholipase D (PLD; EC 3.1.4.4) has been proposed to play a pivotal role in various cellular processes, but molecular understanding of this enzyme is rather limited. This report describes the nucleotide sequence, structure, and genomic organization of a PLD gene from castor bean (Ricinus communis L. cv. Hale). The PLD gene was isolated from a castor bean genomic library using the PLD cDNA as a hybridization probe. Sequence comparison with the PLD cDNA revealed that the PLD gene consisted of four exons and three introns, one of which interrupts the 5'-untranslated region. Southern blot analysis indicated that the cloned PLD gene was present as a single-copy gene, and yet there were other PLD or PLD-related sequences in the castor bean genome.
authors.affiliation: Department of Biochemistry, Kansas State University, Manhattan 66506, USA.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: S J
authors.initials: SJ
authors.lastname: Coughlan
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D002367:Ricinus communis / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D018628:Gene Dosage; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics*; D010947:Plants, Toxic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D010739:Phospholipase D