Cloning and functional characterization of a rat urea transporter: expression in the brain.
A cDNA coding for a rat urea transporter is described. The 1242 bp open reading frame codes for a 414 amino acids protein with 77.0% and 60.7% identity with the human UT11 and the rat UT2, respectively. When expressed in Xenopus oocytes, the protein induces a 10-fold rise in urea permeability, inhibited by mercurial reagents and phloretin. The rUT11 mRNA is massively expressed in the brain.
Couriaud
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C
Service de Biologie Cellulaire, CEA/Saclay, Gif-sur-Yvette, France.
Ripoche
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P
Rousselet
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G
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000235:genetics; D015723:Gene Library; D006031:Glycosylation; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013890:Thiourea / Q000378:metabolism; D014508:Urea / Q000378:metabolism*; D014982:Xenopus laevis
D002352:Carrier Proteins; D012333:RNA, Messenger; D014508:Urea; D013890:Thiourea