[The use of nested polymerase chain reaction method for rapid detection of cytomegalovirus in peripheral blood leukocytes of patients after bone marrow transplantation].
Nested polymerase chain reaction was used for rapid detection of human cytomegalovirus in blood samples of patients subjected to bone marrow transplantation. Use of the four-primer system appreciably improved the sensitivity and specificity of the method and helped eliminate the difficulties arising in the detection of various viral strains with the minor nucleotide substitutes in the DNA template. The proposed method is a reliable tool for the early diagnosis of cytomegaloviral viremia and for monitoring the efficacy of antiviral therapy.
Klincheva
S A
SA
Bakaev
V V
VV
Barinskiĭ
I F
IF
Gnedoĭ
S N
SN
Nikolaeva
N P
NP
D000293:Adolescent; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D015345:Oligonucleotide Probes