An improved method for MN genotyping by the polymerase chain reaction.
A novel method of human MN blood group genotyping is reported using the polymerase chain reaction. Genotyping is based on two base substitutions characteristic of M and N alleles in the 2nd exon of the glycophorin A gene. Using a newly designed primer trio, PCR products for M (255 bp) and N (270 bp) alleles are rapidly and simultaneously detected by a single PCR procedure and subsequent polyacrylamide gel electrophoresis. This method enables MN genotyping from not only minute but also degraded DNA samples.
Nakayashiki
N
N
Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.
Sasaki
Y
Y
D000483:Alleles; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001797:Blood Protein Electrophoresis; D005838:Genotype; D006801:Humans; D008951:MNSs Blood-Group System / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D008951:MNSs Blood-Group System