Study of C-polymorphisms of chromosomes 1, 9 and 16 in lymphocytes of patients with laryngeal carcinoma.
A case-control study of the C-bands of #1, 9 and 16 was performed on PHA-stimulated lymphocytes in 29 individuals, 16 of whom with laryngeal carcinoma. C-bands were revealed using Sumner's CBG staining technique. The assay for chromosomic markers was performed using traditional measuring methods and an image analysis system. The calculation of the heterochromatic index (HI) using Neeley's method was chosen to evaluate size heteromorphisms and was used for each homologue of chromosome pairs examined. No significant difference was found between patients and controls with regard to size polymorphisms. The comparison between patients and controls was significant when size polymorphisms at the level of #9 were taken into account. In fact, 9 out of 16 patients (56%) versus 2 out of 13 controls (15%) presented a partial pericentric inversion on one of the two homologues of chromosome pair 9 (chi 2 = 5.325; 0.05 > p > 0.01). The two measuring techniques produced broadly similar results. However, in our opinion, the classic method is preferable owing to its simplicity of use. In conclusion, we affirm that pericentric inversion of #9 may be regarded as a predisposing factor for the onset of laryngeal carcinoma whose manifestation is triggered by tobacco and alcohol consumption.
Milàn
C
C
Department of Animal Biology, Turin University.
Lamberti
L
L
D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D016022:Case-Control Studies; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002899:Chromosomes, Human, Pair 9; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic