Differences in hemagglutination inhibition activity against bovine rotavirus and hen Newcastle disease virus based on the subunits in hen egg white ovomucin.
The hemagglutination inhibition (HI) activity of ovomucin (OM) subunits, and the effects of alkylation before and after reduction and protease treatments on the HI activity of OM to bovine rotavirus (RV) and hen newcastle disease virus (NDV) were investigated. The appearance of the HI activity of Om against RV was accomplished by a macromolecule composed of alpha- and beta-subunits, while that against NDV required the beta-subunit moiety only.
Tsuge
Y
Y
United Graduate School of Agricultural Science, Gifu University, Japan.
Shimoyamada
M
M
Watanabe
K
K
D000478:Alkylation; D000818:Animals; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D006385:Hemagglutination Inhibition Tests; D009522:Newcastle disease virus / Q000378:metabolism*; D010059:Ovomucin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D012401:Rotavirus / Q000378:metabolism*
D010446:Peptide Fragments; D010059:Ovomucin; D010450:Endopeptidases