title: Distinct regions specify the targeting of otefin to the nucleoplasmic side of the nuclear envelope.
abstract: Otefin is a 45-kDa nuclear envelope protein with no apparent homology to other known proteins. It includes a large hydrophilic domain, a single carboxyl-terminal hydrophobic sequence of 17 amino acids, and a high content of serine and threonine residues. Cytological labeling located otefin on the nucleoplasmic side of the nuclear envelope. Chemical extraction of nuclei from Drosophila embryos revealed that otefin is a peripheral protein whose association with the nuclear envelope is stronger than that of lamin. Deletion mutants of otefin were expressed in order to identify regions that direct otefin to the nuclear envelope. These experiments revealed that the hydrophobic sequence at the carboxyl terminus is essential for correct targeting to the nuclear envelope, whereas additional regions in the hydrophilic domain of otefin are required for its efficient targeting and stabilization in the nuclear envelope.
authors.affiliation: Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, USA.
authors.forename: R
authors.initials: R
authors.lastname: Ashery-Padan
authors.forename: A M
authors.initials: AM
authors.lastname: Weiss
authors.forename: N
authors.initials: N
authors.lastname: Feinstein
authors.forename: Y
authors.initials: Y
authors.lastname: Gruenbaum
mesh_terms: D000818:Animals; D019556:COS Cells; D004330:Drosophila; D029721:Drosophila Proteins; D007763:Lac Operon; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism*; D011487:Protein Conformation; D017384:Sequence Deletion; D012995:Solubility; D013329:Structure-Activity Relationship; D014162:Transfection
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C063595:Ote protein, Drosophila