title: Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2.
abstract: Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite's genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum.
authors.affiliation: Zentrum für Infektionsforschung der Universität Würzburg, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Camargo
authors.forename: K
authors.initials: K
authors.lastname: Fischer
authors.forename: M
authors.initials: M
authors.lastname: Lanzer
mesh_terms: D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular / Q000379:methods; D018628:Gene Dosage; D017125:Genes, Protozoan; D015698:Genomic Library; D010963:Plasmodium falciparum / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere / Q000235:genetics