Anti-peptide antibodies sensitive to the 'active' state of the beta2-adrenergic receptor.
Antibodies directed against a peptide corresponding to the second loop of the human beta2-adrenergic receptor were induced in rabbits by immunisation with the free peptide in complete Freund's adjuvant. The resulting antibodies were affinity-purified and shown to be monospecific for the target receptor. They were able to stimulate the L-type Ca2+ channels in whole-cell patch-clamp experiments on isolated adult guinea-pig cardiomyocytes. This effect was similar to that obtained by the specific beta2-adrenergic agonist zinterol. The antibody effects could be blocked with the specific beta2-adrenergic inverse agonist ICI118,551 but not with the neutral antagonist alprenolol. These results suggest that the antibodies recognise the active conformer of the beta2-adrenergic receptor.
Mijares
A
A
CNRS UMR 6542, Physiologie des Cellules Cardiaques et Vasculaires, Faculté des Sciences, UFR Sciences Pharmaceutiques, Tours, France.
Lebesgue
D
D
Argibay
J
J
Hoebeke
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry / Q000276:immunology*
D000906:Antibodies; D010455:Peptides; D018343:Receptors, Adrenergic, beta-2