Role of interleukin 8 on leucocyte-endothelial cell adhesion in intestinal inflammation.
BACKGROUND
An important action of interleukin 8 (IL8) is stimulation of granulocytes. The object of this study was to assess the contribution of IL8 to the leucocyte-endothelial cell interactions associated with intestinal inflammation in the rat.


METHODS
Two indomethacin injections (48 and 24 hours prior to the experiments) induced a longlasting ileitis in rats. The number of adherent and emigrated leucocytes, leucocyte rolling velocity, and shear rate were monitored in normal and inflamed mesenteric postcapillary venules. Some animals received a monoclonal antibody (MAb) against IL8 or CD11b/CD18 at 24 and 12 hours prior to the experiment.


RESULTS
Indomethacin elicited a seven-fold increase in leucocyte adherence and a 5.4-fold increase in leucocyte emigration, while leucocyte rolling velocity was reduced by nearly 80%. The indomethacin induced increases in leucocyte adherence and emigration were significantly reduced (by 57% and 67%, respectively) while leucocyte rolling velocity was increased (to 63% of control) by the IL8-specific MAb. The level of inhibition seen with the IL8 MAb was similar to that associated with administration of a MAb directed against the leucocyte adhesion molecule CD11b/CD18.


CONCLUSIONS
IL8 contributes to the leucocyte-endothelial cell interactions elicited in mesenteric venules by indomethacin.
Arndt
H
H
Department of Physiology and Biophysics, Louisiana State University Medical Center, USA.
Bolanowski
M A
MA
Granger
D N
DN
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002464:Cell Migration Inhibition; D007079:Ileitis / Q000139:chemically induced / Q000276:immunology*; D007213:Indomethacin / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008642:Mesenteric Veins / Q000276:immunology; D008833:Microcirculation / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D007213:Indomethacin