title: Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2.
abstract: A new propionate (prp) locus of S. typhimurium was defined by mutation, was located to minute 8 of the chromosome, and was shown to be transcribed in the clockwise direction. A plasmid carrying the wild-type prp+ locus was isolated by complementation and its initial physical characterization is presented. Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. Propionate stimulated prp transcription in a merodiploid strain containing prp+ and a prp::MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. prp transcription was reduced by a factor of 2 in strains deficient in the synthesis of the global regulatory protein FruR; fruR mutants failed to grow on propionate. Propionate blocked growth of prp mutants on medium containing succinate as carbon/energy source.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison 53706-1567, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Hammelman
authors.forename: G A
authors.initials: GA
authors.lastname: O'Toole
authors.forename: J R
authors.initials: JR
authors.lastname: Trzebiatowski
authors.forename: A W
authors.initials: AW
authors.lastname: Tsang
authors.forename: D
authors.initials: D
authors.lastname: Rank
authors.forename: J C
authors.initials: JC
authors.lastname: Escalante-Semerena
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007763:Lac Operon; D009154:Mutation; D010641:Phenotype; D011422:Propionates / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014158:Transcription, Genetic
chemical_list: D011422:Propionates; D013386:Succinates; D019802:Succinic Acid