Effect of arsenic (V) on the antioxidant defense system: in vitro oxidation of rat plasma lipoprotein.
Adult male rats were treated orally with sodium arsenate (10 mg As/kd/day) for 2 days, and in increase in hepatic glutathione level was seen. Ascorbic acid content increased in both liver and plasma of intoxicated animals. Hepatic activities of superoxide dismutase and glutathione peroxidase did not change with the treatment and there was no increase in the level of lipid peroxidation measured as thiobarbituric acid-reacting substances (TBARS). Arsenic decreased the plasma level of uric acid and increased the plasma triglycerides content without modifying vitamin E levels. Both total lipoproteins and very low density lipoprotein plus low density lipoprotein (VLDL + LDL) fractions demonstrated greater propensity for in vitro oxidation than the corresponding untreated rats. The last finding might be a useful parameter for determining the degree of oxidative stress in the initial steps of intoxication with arsenic.
Schinella
G R
GR
Department of Pharmacology, Faculty of Medicine, National University of La Plata, Argentina.
Tournier
H A
HA
Buschiazzo
H O
HO
de Buschiazzo
P M
PM
D000818:Animals; D001149:Arsenates / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008074:Lipoproteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D001149:Arsenates; D008074:Lipoproteins; D014810:Vitamin E; C009277:sodium arsenate; D013482:Superoxide Dismutase; D005978:Glutathione