Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Gal alpha 1-3Gal), blood group H determinant and N-glycolylneuraminic acid.
Glycosphingolipids were isolated from primary cultures of porcine endothelial cells labelled with 14C-galactose or 14C-glucosamine. They were characterized by their mobility on thin layer chromatogram, their sensitivity to exoglycosidases, and their labelling with antibodies. In addition to the major glycosphingolipids, globotetra- and globotriaosylceramide, minor ones were identified as penta- and heptaglycosylceramide of the neolactoseries terminated by either Gal alpha 1-3Gal- (xenoreactive epitope) or Fuc alpha 1-2Gal- (H determinant). Two gangliosides were found, GM3 and GD3, and N-glycolylneuraminic acid was their major sialic acid. Therefore, porcine endothelial cells differ from human endothelial cells by expression of glycosphingolipids that are absent in man: two Gal alpha 1-3Gal-terminated glycolipids recognized by human natural antibodies, and two N-glycolylneuraminic acid-terminated gangliosides which are potent immunogens.
Bouhours
D
D
Institut de Transplantation et de Recherche en Transplantation, INSERM U.437, Centre Hospitalier Régional, Nantes, France.
Pourcel
C
C
Bouhours
J E
JE
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D001011:Aorta / Q000166:cytology* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004730:Endothelium, Vascular / Q000378:metabolism*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005944:Glucosamine / Q000737:chemistry / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000276:immunology*; D013552:Swine
D000017:ABO Blood-Group System; D000939:Epitopes; D005679:G(M3) Ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; C026226:ganglioside, GD3; D005944:Glucosamine; D005690:Galactose