Visualization of antigen-specific T cell activation and cytokine expression in vivo.
A transgenic TCR adoptive transfer system was used to visualize Ag-specific T cell activation and cytokine expression in vivo. After s.c. injection of peptide in adjuvant the entire Ag-specific population up-regulated IL-2 receptor alpha-chain expression, underwent blast transformation, and developed a memory-surface phenotype. A minority of the Ag-specific T cells produced predominantly IL-2 mRNA and localized at the T cell/B cell junction in draining lymph nodes. In the secondary response, a mixed cytokine pattern of both Th1 and Th2 cytokines was demonstrated. When peptide was administered i.v. without adjuvant, 50% of the Ag-specific cells expressed IL-2, but the peak of expression occurred before IL-2 receptor alpha-chain up-regulation, and only a minority of the Ag-specific T cells underwent blast transformation.
Rogers
W O
WO
Department of Pathology, University of Alabama at Birmingham, 35233, USA.
Weaver
C T
CT
Kraus
L A
LA
Li
J
J
Li
L
L
Bucy
R P
RP
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004333:Drug Administration Routes; D005434:Flow Cytometry; D007114:Immunization / Q000379:methods; D017403:In Situ Hybridization; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008954:Models, Biological; D010047:Ovalbumin / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
D016207:Cytokines; D012333:RNA, Messenger; D010047:Ovalbumin