Identification of two pathogenic avian mycoplasmas as strains of Mycoplasma pullorum.
Two mycoplasma strains were isolated from dead turkey embryos. Growth properties, biochemical and serological characteristics, and protein profiles indicated that these strains were closely related to Mycoplasma pullorum CKKT (T = type strain). This was confirmed by 16S rRNA sequence analysis, and the phylogenetic position of M. pullorum was established. Pathogenicity studies showed that the two strains, as well as M. pullorum CKKT, induced a statistically significant level of mortality after inoculation into chicken embryos.
Moalic
P Y
PY
Unité de Recherches Mycoplasmologie-Bactériologie, Centre Nationale d'Etudes Vétérinaires et Alimentaires, Ploufragan, France.
Kempf
I
I
Gesbert
F
F
Laigret
F
F
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D002642:Chick Embryo; D003470:Culture Media / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014422:Turkeys
D001426:Bacterial Proteins; D003470:Culture Media; D012336:RNA, Ribosomal, 16S