Expression of differentiation antigens and adhesion molecules on CD34+ cells in peripheral blood and bone marrow after chemotherapy followed by administration of granulocyte colony stimulating factor.
The expression of differentiation antigens (D/As) and adhesion molecules (A/Ms) was studied on CD34 positive (CD34+) cells in peripheral blood (PB) and bone marrow (BM) of patients treated with myelosuppressive chemotherapy followed by granulocyte colony-stimulating factor (G-CSF) administration (CT/G-CSF). CD33 positive rates of PB and BM CD34+ cells were significantly higher in the patients than those in the normal controls. CD49d positive rate of PB CD34+ was significantly lower than that of BM CD34+ cells in both the patients and the normal controls, and this decreased expression of CD49d on PB CD34+ cells was larger in the patients than in the normal controls. CD117 positive rate was significantly lower in PB CD34+ cells of the patients than that of their BM CD34+ cells and PB CD34+ cells of the normal control. These results suggested that more mature progenitor cells increased in the patients' PB and BM after CT/G-CSF, and that the decreased expression of CD49d played an important role on peripheralisation of CD34+ cells even in steady state, and the decreased expression of CD117 was related with the mobilization of CD34+ cells after CT/G-CSF. CT/G-CSF decreased the expression of CD117 and enhance the decrease of CD49d expression on PB CD34+ cells. These changes appeared to be involved in the mobilization mechanism of hematopoietic progenitor cells from BM into PB after CT/G-CSF.
Masauzi
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Third Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Japan.
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis* / Q000502:physiology; D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000502:physiology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006402:Hematologic Diseases / Q000097:blood / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D039441:Integrin alpha4; D008297:Male; D008875:Middle Aged; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis* / Q000502:physiology
D015703:Antigens, CD; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D016179:Granulocyte Colony-Stimulating Factor; D039441:Integrin alpha4; D019009:Proto-Oncogene Proteins c-kit