title: Association of herpes simplex virus regulatory protein ICP22 with transcriptional complexes containing EAP, ICP4, RNA polymerase II, and viral DNA requires posttranslational modification by the U(L)13 proteinkinase.
abstract: The expression of herpes simplex virus 1 gamma (late) genes requires functional alpha proteins (gamma1 genes) and the onset of viral DNA synthesis (gamma2 genes). We report that late in infection after the onset of viral DNA synthesis, cell nuclei exhibit defined structures which contain two viral regulatory proteins (infected cell proteins 4 and 22) required for gamma gene expression, RNA polymerase II, a host nucleolar protein (EAP or L22) known to be associated with ribosomes and to bind small RNAs, including the Epstein-Barr virus small nuclear RNAs, and newly synthesized progeny DNA. The formation of these complexes required the onset of viral DNA synthesis. The association of infected cell protein 22, a highly posttranslationally processed protein, with these structures did not occur in cells infected with a viral mutant deleted in the genes U(L)13 and U(S)3, each of which specifies a protein kinase known to phosphorylate the protein.
authors.affiliation: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Illinois 60637, USA.
authors.forename: R
authors.initials: R
authors.lastname: Leopardi
authors.forename: P L
authors.initials: PL
authors.lastname: Ward
authors.forename: W O
authors.initials: WO
authors.lastname: Ogle
authors.forename: B
authors.initials: B
authors.lastname: Roizman
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006561:Herpes Simplex / Q000821:virology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012269:Ribosomal Proteins; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; C057294:ICP22 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C048925:herpes simplex virus, type 1 protein ICP4; C067169:RPL22 protein, human; C078424:EUS1 protein, Equine herpesvirus 1; D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus; D012319:RNA Polymerase II