[Contribution of Trichrome Blue in the diagnosis of microsporidiosis].
Detection of microsporidia belongs to the usual coprologic and urine detection of parasites from HIV seropositive patients. To improve the identification of microsporidial spores, several stains have been used. Trichrome Blue stain has been evaluated in this study. We first compared Trichrome Blue stain to Weber's trichrome for the detection of microsporidia in smears of stools received from HIV seropositive patients. No difference of sensibility has been demonstrated between the two stains, and Uvitex 2B used on the same samples has confirmed these results. Then, Trichrome Blue stain has been used for the detection of microsporidial spores in other specimens (40 samples of nasal mucus, conjonctival samples, duodenal biopsy and urine), also Giemsa and Uvitex 2B. The advantage of Trichrome Blue stain is its ready-to-use presentation, and faster realisation at higher temperature. Trichrome Blue stain is interesting as a confirmation technique or for laboratories which do not have fluorescent microscopy equipment.
Honoré
P S
PS
Laboratoire de parasitologie, Hôpital Saint-Louis, Paris.
Houze
S
S
Sarfati
C
C
Challier
S
S
Kac
G
G
Le Bras
J
J
Derouin
F
F
D000818:Animals; D001391:Azo Compounds; D004396:Coloring Agents; D003228:Conjunctiva / Q000469:parasitology; D004386:Duodenum / Q000469:parasitology; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000469:parasitology; D006679:HIV Seropositivity / Q000469:parasitology; D006801:Humans; D008739:Methyl Green; D016816:Microsporida / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis* / Q000469:parasitology; D009093:Mucus / Q000469:parasitology; D009666:Nose; D013170:Spores / Q000302:isolation & purification; D013194:Staining and Labeling; D014556:Urine / Q000469:parasitology
D001391:Azo Compounds; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)