Bio-antimutagenic activity of green tea catechins in cultured Chinese hamster V79 cells.
The antimutagenic effects of green tea catechins, (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg) on induction of 6-thioguanine (6TG)-resistant mutations induced by 4-nitroquinolin 1-oxide (4NQO) were found in cultured Chinese hamster V79 cells. The antimutagenic activity of catechins was found only when cells were post-treated with catechins during the mutation expression time after treatment with 4NQO, and not found by simultaneous treatments with 4NQO and catechins. This bioantimutagenic activity of catechins were not observed in ethyl methanesulfonate (EMS)-induced mutations. This suggests that the antimutagenic effects of catechins may act intracellularly as bio-antimutagenic blocking agent or suppressive agent. These catechins had no effects on the cytotoxic activity of 4NQO in V79 cells, whether catechins were used in simultaneous treatment with or in post-treatment after 4NQO. This indicates that the antimutagenicity and anticytotoxicity to 4NQO may be caused by different mechanism(s).
Kuroda
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Department of Environmental Health Science, Azabu University, Kanagawa, Japan.
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D007928:Lethal Dose 50; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D013662:Tea / Q000737:chemistry*
D016587:Antimutagenic Agents; D009153:Mutagens; D013662:Tea; D015112:4-Nitroquinoline-1-oxide; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate