title: The amino acid substitutions Ser288Gly and Val320Ala convert the cortisol producing enzyme, CYP11B1, into an aldosterone producing enzyme.
abstract: Transfection studies with cDNAs encoding hybrids between the highly similar cytochrome P450 enzymes, CYP11B1 (steroid 11 beta-hydroxylase) and CYP11B2 (aldosterone synthase) have identified which amino acids determine the different activities of the enzymes.
authors.forename: K M
authors.initials: KM
authors.lastname: Curnow
authors.forename: P
authors.initials: P
authors.lastname: Mulatero
authors.forename: N
authors.initials: N
authors.lastname: Emeric-Blanchouin
authors.forename: B
authors.initials: B
authors.lastname: Aupetit-Faisant
authors.forename: P
authors.initials: P
authors.lastname: Corvol
authors.forename: L
authors.initials: L
authors.lastname: Pascoe
mesh_terms: D000450:Aldosterone / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics*; D018076:DNA, Complementary; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis*; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013252:Steroid 11-beta-Hydroxylase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D000450:Aldosterone; D013252:Steroid 11-beta-Hydroxylase; D006854:Hydrocortisone