cDNA cloning and chromosomal localization of the human telencephalin and its distinctive interaction with lymphocyte function-associated antigen-1.
We have isolated cDNA encoding human telencephalin (TLN), a brain segment-specific neuronal adhesion molecule. Human TLN comprises an NH2-terminal signal peptide, an extracellular region with nine Ig-like domains, a single transmembrane region, and a COOH-terminal cytoplasmic tail. The NH2-terminal five Ig-like domains of TLN were closely related to those of intercellular adhesion molecules (ICAMs)-1 and -3. The TLN gene was mapped to the human chromosome 19p13.2, where the ICAM-1, -3, and -4 (LW) genes are located. Furthermore, we observed lymphocyte function-associated antigen-1 (LFA-1)-mediated adhesion of HL-60 cells on recombinant TLN protein, as well as on ICAM-1. However, the interaction of TLN with LFA-1 on HL-60 cells was divalent cation-independent and phorbol 12-myristate 13-acetate stimulation-independent. We conclude that TLN is a unique neuronal member of ICAM subgroup of the Ig superfamily and propose a novel type of interaction between the Ig superfamily molecule and integrin, which does not require the activation of integrin. TLN on the surface of telencephalic neurons may be a target molecule in the brain for LFA-1-expressing microglia and leukocytes in physiological or pathological conditions.
Mizuno
T
T
Department of Neuroscience, Osaka Bioscience Institute, Suita, Japan.
Yoshihara
Y
Y
Inazawa
J
J
Kagamiyama
H
H
Mori
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry; D000943:Antigens, Differentiation; D001483:Base Sequence; D001789:Blood Group Antigens / Q000737:chemistry; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002450:Cell Communication; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D018076:DNA, Complementary / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000166:cytology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D011817:Rabbits; D016415:Sequence Alignment
D015703:Antigens, CD; D000943:Antigens, Differentiation; D001789:Blood Group Antigens; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C065289:ICAM2 protein, human; C483616:ICAM3 protein, human; C095100:ICAM4 protein, human; C086747:ICAM5 protein, human; C504585:Icam5 protein, mouse; D007136:Immunoglobulins; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D018799:Intercellular Adhesion Molecule-1