Isolation of the mouse aldose reductase promoter and identification of a tonicity-responsive element.
Aldose reductase (AR; EC 1.1.1.21) is an oxidoreductase that catalyzes the NADPH-dependent conversion of glucose to sorbitol, the first step of the polyol pathway. AR is of great interest due to its implication in the etiology of diabetic complications. In renal medullary cells, AR also plays an osmoregulatory role by accumulating sorbitol to maintain the intracellular osmotic balance during antidiuresis. We have previously cloned the AR cDNA from mouse kidney, and we report here the isolation of the mouse AR gene promoter. Transient transfection of chloramphenicol acetyltransferase reporter constructs containing various 5'-flanking regions of the mouse AR gene in CV1 cells led to the identification of a sequence spanning base pairs -1053 to -1040, required for an enhancer activity in hypertonic compared with isotonic cell culture conditions. This sequence is similar to the tonicity-responsive element first characterized in the betaine-gamma-aminobutyric acid transporter promoter.
Daoudal
S
S
Laboratoire de Reproduction et Développement, CNRS, URA 1940, Université Blaise Pascal-Clermont-Ferrand II, 63177 Aubiere Cédex, France.
Tournaire
C
C
Halere
A
A
Veyssière
G
G
Jean
C
C
D000449:Aldehyde Reductase / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003001:Cloning, Molecular; D017931:DNA Primers; D007438:Introns; D007679:Kidney Medulla / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection
D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D000449:Aldehyde Reductase; D015500:Chloramphenicol O-Acetyltransferase