Functional promoter elements common to ribosomal protein and ribosomal RNA genes in Neurospora crassa.
The promoter sequences of a cytoplasmic ribosomal protein gene (crp-2) of Neurospora crassa were identified using promoter deletion and substitution mutants. A gene-targeting strategy was used to assay the mutants in vivo. The promoter architecture of crp-2 is complex and is more similar to that of ribosomal protein genes in mouse than in Saccharomyces cerevisiae. Six regions were identified as important for transcription. These included two elements, a CG repeat and a Dde box, that are conserved in most other promoters of N. crassa ribosomal protein genes and have also been demonstrated as being required for transcription from the 40 S rRNA promoter by RNA polymerase I in vitro. The CG repeats located at -73 to -66 and between -189 and -154 were functionally redundant and increased transcription efficiency by 10- to 15- fold. The Dde boxes located at -153 to -147 and at -95 to -83 contributed 2-fold and 5-fold to transcription efficiency, respectively. An unidentified element between -254 and -190 contributed 2-fold, while a pyrimidine-rich region between -85 and -66 influenced the start point of transcription.
Cujec
T P
TP
Department of Plant Pathology, University of California, Davis 95616, USA.
Tyler
B M
BM
D001483:Base Sequence; D017124:Conserved Sequence; D005656:Fungal Proteins / Q000235:genetics; D018390:Gene Targeting; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009492:Neurospora crassa / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D012407:rRNA Operon
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins