Nuclear and cytoplasmic actin in dinoflagellates.
Experiments using monoclonal and polyclonal anti-actin antibodies allowed us to demonstrate the presence of F- or G-actin in original protists, dinoflagellates, either by biochemistry, immunofluorescence and in TEM. SDS-PAGE electrophoresis and immunoblottings made either from total or nuclear protein extracts revealed the presence of a 44-kDa band reacting with monoclonal anti-actin antibody in two species, Prorocentrum micans and Crypthecodinium cohnii, and thus demonstrated the presence of actin in nuclear and cytoplasmic fractions. After squash preparation of P micans cells, actin was identified within the nucleus and in some regions of the cytoplasm by immunofluorescence microscopy. Labelling of both the nucleolus and the centrosome region was evident together with amorphous nucleoplasmic material surrounding the chromosomes. The use of cryosections of intact P micans and C cohnii cells for immunofluorescence along with staining with DAPI to delineate the chromosomes themselves, yielded finer resolution of the intranuclear network labelling pattern and allowed us to complete our observations, in particular on the cytoplasmic labelling. In P micans, in addition to the centrosome region, the cytoplasmic channels passing through the nucleus in dividing cells are labelled. In C cohnii, the cortex, the centrosome region, the cytoplasmic channels, the region surrounding the nucleus, the filaments linking it to the cortex and the cleavage furrow are also labelled. In the nucleus of the two species, there is a prominent "weft' of fine actin filaments in the nucleoplasm forming a matrix of varying density around the persistent chromosomes. This actin matrix, of unknown function, is most conspicuous at the end of the S-phase of the cell cycle. Fluorescent derivatives of phalloidin, used as diagnostic cytochemical probes for polymeric actin (F-actin), gave similar results. Positive TEM immunolabelling of intranuclear actin confirms its presence in the nucleoplasm, in the nucleolus where the preribosomal region is labelled while C cohnii chromosomes are unlabelled and the P micans chromosomes very slightly. In the cytoplasm, lips of the cleavage furrow and kinetosome regions are labelled as well as the centrosome region. The possible functions of this protein located in several compartments of dinoflagellate cells are discussed.
Soyer-Gobillard
M O
MO
Départment de Biologie Cellulaire, Observatoire Océanologique de Banyuls-sur-mer, CNRS-URA No 2156, Université Paris 6, Laboratoire Arago, Banyuls-sur-mer, France.
Ausseil
J
J
Géraud
M L
ML
D000199:Actins / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry* / Q000648:ultrastructure; D004141:Dinoflagellida / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D007399:Interphase / Q000502:physiology; D008854:Microscopy, Electron; D010590:Phalloidine
D000199:Actins; D000911:Antibodies, Monoclonal; D010590:Phalloidine