The MHC-encoded TAP1/LMP2 bidirectional promoter is down-regulated in highly oncogenic adenovirus type 12 transformed cells.
Cells transformed by human adenovirus 12 (Ad12) exhibit extremely low surface levels of MHC class I molecules and contain reduced levels of class I heavy chain mRNAs. We report that levels of MHC-encoded TAP1 and LMP2 mRNAs are also down-regulated in Ad12-transformed rat cells, and that transcription of rat TAP1 and LMP2 transcripts is directed from a 564 bp intergenic region which is significantly less active in Ad12-transformed cells compared to those transformed with Ad5. Our results suggest that, in common with MHC class I gene expression, TAP1 and LMP2 gene expression is reduced mainly at the level of transcription in Ad12-transformed cells.
Proffitt
J A
JA
Department of Biochemistry and Molecular Biology, University of Leeds, UK.
Blair
G E
GE
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases; D018076:DNA, Complementary; D015536:Down-Regulation; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C073119:TAP1 protein, human; C486172:Tap1 protein, rat; C071313:LMP-2 protein; D003546:Cysteine Endopeptidases