Autologous connective tissue chamber as a tool for introducing active substances into the CNS.
A new method of introducing active substances into the CNS is described. The autologous connective tissue chambers were obtained by implantation of a silicone tube under the back skin of rats. Subsequently they were filled with fibrine and additionally with NGF or submicrosomal fractions from nonpredegenerated and predegenerated peripheral nerves. Filled chambers were implanted stereotaxically into the injured hippocampus. The neurite outgrowth was examined by means of FITC-HRP and acetylcholinesterase-method. Implanted connective tissue chambers are very useful in getting active substances into the CNS. This method allows to avoid inflammatory processes and does not hinder the histological procedures.
Barski
J J
JJ
Department of Physiology, Silesian Medical School, Katowice, Poland.
Lewin-Kowalik
J
J
Krause
M
M
Gołka
B
B
Górka
D
D
Larysz-Brysz
M
M
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology*; D002491:Central Nervous System Agents / Q000008:administration & dosage*; D003238:Connective Tissue / Q000294:innervation / Q000502:physiology* / Q000637:transplantation; D004343:Drug Implants; D006133:Growth Substances / Q000494:pharmacology; D006624:Hippocampus / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000201:enzymology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014020:Tissue Extracts / Q000494:pharmacology; D014182:Transplantation, Autologous
D002491:Central Nervous System Agents; D004343:Drug Implants; D006133:Growth Substances; D014020:Tissue Extracts; D000110:Acetylcholinesterase