title: Studies on high-density cultivation of Vero cells with biosilon solid microcarrier.
abstract: From eight kinds of microcarriers, Biosilon has been chosen as the most suitable microcarrier for high-density cultivation of African green monkey kidney (Vero) cells. Conditions affecting high-density cultivation were studied with a 500 ml modified Wheaton spinner flask. When cultivation with a 1.5 L CelliGen bioreactor at inoculum density of 4.9 x 10(6)/ml and perfusion volume increased from 0.20 L to 3.65 L gradually, the cell density could reach 2.05 x 10(7)/ml after 22 days of cultivation. When Vesicular Stomatitis Virus (VSV) was inoculated into the cultures of 1.21 x 10(6) ml at the some MOI (multiplicity of infection), the virus titers reached 7 log TCID50/ml and 8 log TCID50/ml, respectively.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D019149:Bioreactors; D002448:Cell Adhesion; D002452:Cell Count; D002465:Cell Movement; D002522:Chlorocebus aethiops; D046508:Culture Techniques / Q000379:methods*; D011137:Polystyrenes; D014709:Vero Cells / Q000821:virology; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014776:Virus Cultivation / Q000379:methods
chemical_list: D011137:Polystyrenes; C024075:styrofoam