Sodium current amplitude increases dramatically in human retinal glial cells during diseases of the eye.
Müller cells, the main macroglial cells of the retina, express several types of voltage and ligand-activated ion channels, including Na+ channels. Using the whole-cell voltage-clamp technique, we studied the expression of Na+ currents in acutely isolated, non-cultivated human Müller cells from retinas of healthy organ donors and patients suffering from different eye diseases. In both types of retinas transient Na+ currents could be recorded from Müller cells. The tetrodotoxin-resistant Na+ currents, which were not completely blocked even at a concentration of 10 microM tetrodotoxin, had a mean current density of 3.0 +/- 3.0 pA/pF (mean +/- SD, n = 10) in Müller cells from donor retinas and of 12.2 +/- 9.6 pA/pF (n = 74) in Müller cells from patient retinas. Only 33.3% of healthy but 88.4% of pathological Müller cells depicted such currents. The GNa+/GK+ ratio was very high in several Müller cells from patient retinas, such that action potential-like activity could be generated after prehyperpolarizing current injection in some of these cells. Apparently, the Na+ channels, due to their negative steady-state inactivation curve (Vh = -84.5 mV), do not influence the lowered membrane potential of the pathological cells, since they are inactivated at these voltages. Currently, we do not have an explanation for the increase in amplitude and frequency of Na+ currents in human Müller cells under pathological conditions. However, the up-regulation of Na+ channels may mirror a basic glial response to pathological conditions, since it has also been found previously in human hippocampal astrocytes from epileptic foci and in rat cortex stab wounds lined by an astrocytic scar.
Francke
M
M
Paul Flechsig Institute for Brain Research, Department of Neurophysiology, University of Leipzig, Germany.
Pannicke
T
T
Biedermann
B
B
Faude
F
F
Reichelt
W
W
D004553:Electric Conductivity; D005128:Eye Diseases / Q000473:pathology / Q000503:physiopathology*; D006706:Homeostasis; D006801:Humans; D007700:Kinetics; D009457:Neuroglia / Q000502:physiology*; D012016:Reference Values; D012160:Retina / Q000473:pathology / Q000503:physiopathology*; D012530:Saxitoxin / Q000494:pharmacology; D012964:Sodium / Q000502:physiology*; D026941:Sodium Channel Blockers; D015222:Sodium Channels / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology
D026941:Sodium Channel Blockers; D015222:Sodium Channels; D012530:Saxitoxin; D013779:Tetrodotoxin; D012964:Sodium