Acid-fastness of Nocardia asteroides GUH-2 cultured in brain heart infusion broth supplemented with paraffin.
Nocardia asteroides GUH-2 was not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth supplemented with paraffin, many filamentous cells showed acid-fastness after treatment with 1% acid-alcohol as the decolorizing agent. When treated with 3% acid-alcohol, filamentous cells were not acid-fast. In addition to the acid-fast filamentous cells of nocardiae, unknown acid-fast spherical bodies were observed in the paraffin-supplemented BHI broth cultures.
Kohbata
S
S
Department of Microbiology, Gifu University School of Medicine, Japan.
D004396:Coloring Agents; D003470:Culture Media; D009616:Nocardia asteroides / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D010232:Paraffin / Q000378:metabolism*; D013194:Staining and Labeling
D004396:Coloring Agents; D003470:Culture Media; D010232:Paraffin