Routine determination of flumequine in kidney tissue of pig using automated liquid chromatography.
A high-performance liquid chromatographic assay is described as a routine analytical method for the determination of flumequine (FLU) and its hydroxylated metabolite (OH-FLU) in pig kidney tissue. Kidney samples (2 g) containing FLU and OH-FLU were extracted by liquid-liquid extraction with ethyl acetate (10 ml). Analytical separations were performed by reversed-phase HPLC with fluorometric detection at 252 nm excitation and 356 nm emission under gradient conditions. The mobile phase was acetonitrile-2.7.10(-3) M oxalic acid in water (pH 2.5). The assay is specific and reproducible within the flumequine range of 0.050-2.5 micrograms/g and recovery at 0.050 microgram/g was 94.8%.
Guyonnet
J
J
Department of Pharmacokinetics and Drug Metabolism, Sanofi Santé Nutrition Animale, Libourne, France.
Pacaud
M
M
Richard
M
M
Doisi
A
A
Spavone
F
F
Hellings
P
P
D000284:Administration, Oral; D000818:Animals; D000892:Anti-Infective Agents, Urinary / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D024841:Fluoroquinolones; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D011807:Quinolizines / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013552:Swine
D000892:Anti-Infective Agents, Urinary; D024841:Fluoroquinolones; D011807:Quinolizines; C012976:flumequine