title: Effect of the sugar chain of soluble recombinant CD59 on complement inhibitory activity.
abstract: A soluble recombinant CD59#77 (rCD59#77), consisting of 77 amino acids starting from the N terminus of membrane-bound CD59, was prepared using a gene expression system in CHO cells. The rCD59#77 preparation was composed of glycosylated and non-glycosylated forms (G and NG forms). Unexpectedly, NG form was 7 times more potent than G form in complement inhibitory activity. Postulating that sialic acids on G-form molecules make it difficult for rCD59#77 to access nascent membrane attack complexes on the cell surface, the sialic acids were removed by neuraminidase treatment. However, the inhibitory activity was not changed. Next, one of two putative N-glycosylation sites was mutated by substituting Gln18 for Asn18. The mutant, designated rCD59#77(N/Q), had no sugar moiety and was as active as the NG form of rCD59#77. These results suggest that the bulky sugar moiety at Asn18 is not necessary for the complement-inhibitory activity of rCD59 and actually hampers that function.
authors.affiliation: Molecular Medicine Research Laboratory, Yamanouchi Pharmaceutical Co., Ltd., Tsukuba City, Ibaraki, Japan. suzuk_hi@yamanouchi.co.jp
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Yamaji
authors.forename: A
authors.initials: A
authors.lastname: Egashira
authors.forename: K
authors.initials: K
authors.lastname: Yasunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Sugita
authors.forename: Y
authors.initials: Y
authors.lastname: Masuho
mesh_terms: D000818:Animals; D018957:CD59 Antigens / Q000737:chemistry*; D016466:CHO Cells; D003169:Complement Inactivator Proteins / Q000737:chemistry*; D006224:Cricetinae; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D018957:CD59 Antigens; D003169:Complement Inactivator Proteins; D011994:Recombinant Proteins