cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen.
A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature processed protein of 102 aa with a molecular mass of 11344 Da. The expressed recombinant protein, named rPar j 2.0101, was a major allergen since it reacted with IgE of 82% (23/28) of the sera of Pj-allergic subjects analyzed. It was shown to be a new allergen since (i) the amino acid sequence homology with the already reported recombinant allergen Par j 1.0101 was 45% and (ii) there was no cross-inhibition between rPar j 2.0101 and rPar j 1.0101. In addition, rPar j 2.0101 inhibited 35% of the specific IgE for 10-14 kDa native allergens and preincubation of sera from Pj-allergic patients with both rPar j 2.0101 and rPar j 1.0101 fully abolished the IgE recognition of the 10-14 kDa native allergen region, suggesting that these two allergens contributed to the region.
Duro
G
G
Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
Colombo
P
P
Costa
M A
MA
Izzo
V
V
Porcasi
R
R
Di Fiore
R
R
Locorotondo
G
G
Mirisola
M G
MG
Cocchiara
R
R
Geraci
D
D
D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D052179:Antigens, Plant; D018076:DNA, Complementary; C103299:Par j 2.0101; D010940:Plant Proteins; D021382:Protein Sorting Signals