Albumin can reverse the release of potassium from human erythrocytes treated with the non-ionic detergent, Brij 58.
Exchange of erythrocyte intracellular (i/c) K+ for extracellular (e/c) Na+ in human erythrocytes treated with sub-CMC concentrations of the non-ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K+ contents of detergent-treated human erythrocytes with the incubation medium was reversed by an albumin-mediated withdrawal of detergent molecules from the cell. Re-establishment of near normal [K+] in terms of K+/kg water proceeds in two ways: (i) a metabolism-dependent net accumulation of K+ ions; and (ii) a metabolism-independent shrinkage of erythrocytes, this being the more significant factor.
Bogner
P
P
Diagnostic Center, Pannon University of Agriculture, Faculty of Animal Production, Kaposvár, Hungary.
Wheatley
D N
DN
Borbély
C
C
Miseta
A
A
D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D002463:Cell Membrane Permeability / Q000187:drug effects; D048429:Cell Size; D002592:Cetomacrogol / Q000494:pharmacology*; D002712:Chlorides / Q000097:blood; D003470:Culture Media / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004734:Energy Metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005454:Fluorescence Polarization; D006801:Humans; D007773:Lactates / Q000097:blood; D010042:Ouabain / Q000494:pharmacology; D010938:Plant Oils; D011092:Polyethylene Glycols / Q000494:pharmacology; D011188:Potassium / Q000097:blood*; D012709:Serum Albumin / Q000494:pharmacology*; D012964:Sodium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000097:blood*; D013501:Surface-Active Agents / Q000494:pharmacology*
D001786:Blood Glucose; D002712:Chlorides; D003470:Culture Media; D004791:Enzyme Inhibitors; D007773:Lactates; D010938:Plant Oils; D012709:Serum Albumin; D013501:Surface-Active Agents; D003571:Cytochalasin B; D011092:Polyethylene Glycols; D010042:Ouabain; D000255:Adenosine Triphosphate; D002592:Cetomacrogol; C033084:polyethylene glycol oleyl ether; C043444:octadecyl polyoxyethylene ether; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium