Optimizing expression of transgenes with an emphasis on post-transcriptional events.
Introducing a foreign gene into a new plant host does not always result in a high level of expression of the incoming gene. Numerous promoters have been used to express foreign genes in different plant tissues, but there are sometime various features of the new gene which are deleterious to expression in the new host. There are a number of post-transcriptional steps in the expression of a gene and sometimes sequences present in a particular coding region can resemble the signals which initiate these processing steps. When aberrantly carried out, these steps diminish the level of expression. By removing such fortuitous signals, one can dramatically increase expression of a transgene in plants. Ensuring proper protein folding and/or targeting the protein product to a particular cellular compartment can also be used to increase the level of protein obtained. The various methods used to optimize expression of a foreign gene in plants by concentrating on post-transcriptional events are discussed.
Koziel
M G
MG
Ciba Agricultural Biotechnology Unit, Research Triangle Park, NC 27709, USA.
Carozzi
N B
NB
Desai
N
N
D002736:Chloroplasts / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D007438:Introns; D017510:Protein Folding; D012323:RNA Processing, Post-Transcriptional; D019076:Transgenes