Control of the availability of exogenous 5-aminolevulinic acid in Escherichia coli.
We observed novel phenomena on the growth of a hemA deletion mutant (H500) of Escherichia coli: First, H500 cells grown in rich medium required an increased amount of exogenous 5-aminolevulinic acid (ALA) for normal growth, as compared with cells grown in poor medium. Second, H500 cells grown with an insufficient supply of ALA had a prolonged lag phase but relatively normal growth during the logarithmic phase. An increased requirement of exogenous ALA in rich media was due to an impermeability of the cells to ALA, resulting in a reduced ability to synthesize porphyrins. The latter could be also explained by change in permeability that was repressed under heme-deficient conditions.
Nakayashiki
T
T
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Inokuchi
H
H
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017353:Gene Deletion; D011166:Porphyrins / Q000096:biosynthesis
D003470:Culture Media; D011166:Porphyrins; D000622:Aminolevulinic Acid; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase