Is the lectin binding pattern of human breast and colon cancer cells influenced by modulators of sialic acid metabolism?
Sialic acid residues are the most abundant terminal carbohydrate residues of mammalian cells. Modification of the sialic acid residues by exposure of cells in culture to sialic acid precursor analogues resulted in a modified susceptibility to polyoma viruses. In the present study, human breast and colon cancer cell lines were exposed for 65 h to these acid precursor analogues at 5 mM and their lectin binding pattern was analysed. Use of a panel of several different lectins indicated that the pretreatment of these cell lines with the sialic acid analogues did not change their lectin binding profile. The incorporation of these precursors into membrane glycoproteins was assessed by reversed phase high-performance liquid chromatography, which clearly demonstrated that the precursors were incorporated. The results therefore indicate that these analogues are highly specific for sialic acid and do not interfere with other biosynthetic pathways of membrane glycoconjugates.
Schumacher
U
U
University of Southampton, UK.
Mukhtar
D
D
Stehling
P
P
Reutter
W
W
D001943:Breast Neoplasms; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms; D005260:Female; D005456:Fluorescent Dyes; D006023:Glycoproteins / Q000378:metabolism; D019073:HT29 Cells / Q000737:chemistry / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009438:Neuraminic Acids / Q000032:analysis / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology
D005456:Fluorescent Dyes; D006023:Glycoproteins; D037102:Lectins; D009438:Neuraminic Acids; D019158:N-Acetylneuraminic Acid