Negative regulation of immediate-early gene expression of pseudorabies virus by interferon-alpha.
Pseudorabies rabies (PrV) replication in Vero cells was suppressed by treatment with human natural interferon-alpha (IFN-alpha). Messenger RNA transcribed from the PrV immediate-early (IE) gene was reduced in the IFN-alpha-treated cells. Transient expression assays showed that transcription from the PrV IE promoter was selectively inhibited in the IFN-alpha-treated cells. Analysis of deletion mutants of the PrV IE promoter sequence suggested that at least one element between the transcription initiation site (+1) and -90 in the PrV IE promoter was concerned with the negative regulation.
Tonomura
N
N
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
Ono
E
E
Shimizu
Y
Y
Kida
H
H
D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D017781:Genes, Immediate-Early / Q000187:drug effects*; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007700:Kinetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
D016898:Interferon-alpha; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase