title: Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome.
abstract: Elongation factor G (EF-G) is a GTPase that is involved in the translocation of bacterial ribosomes along messenger RNA during protein biosynthesis. In contrast to current models, EF-G-dependent GTP hydrolysis is shown to precede, and greatly accelerate, the rearrangement of the ribosome that leads to translocation. Domain IV of the EF-G structure is crucial for both rapid translocation and subsequent release of the factor from the ribosome. By coupling the free energy of GTP hydrolysis to translocation, EF-G serves as a motor protein to drive the directional movement of transfer and messenger RNAs on the ribosome.
authors.affiliation: Institute of Molecular Biology, University of Witten/Herdecke, Germany.
authors.forename: M V
authors.initials: MV
authors.lastname: Rodnina
authors.forename: A
authors.initials: A
authors.lastname: Savelsbergh
authors.forename: V I
authors.initials: VI
authors.lastname: Katunin
authors.forename: W
authors.initials: W
authors.lastname: Wintermeyer
mesh_terms: D001665:Binding Sites; D001692:Biological Transport; D001696:Biomechanical Phenomena; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D017384:Sequence Deletion; D014756:Viomycin / Q000494:pharmacology
chemical_list: D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D020963:GTP Phosphohydrolase-Linked Elongation Factors; D014756:Viomycin