DNA strand breaks in testicular cells from humans and rats following in vitro exposure to 1,2-dibromo-3-chloropropane (DBCP).
Preparations of testicular cells from human organ transplant donors and from Wistar rats were compared with respect to their composition of the different testicular cell types, their ability to metabolize 1,2-dibromo-3-chloropropane (DBCP), and their relative sensitivity to induction of DNA single strand breaks and alkali labile sites (ssDNA breaks) after treatment with DBCP, 4-nitroquinoline N-oxide (4-NQO), and X rays. Flow cytometric and microscopic analysis demonstrated that the interindividual variation in the composition of testicular cell types was considerably greater in the human tissue than in that from rats. The in vitro metabolic activation of DBCP (50 to 250 microM), measured as radiolabel covalently bound to macromolecules, was three-fold faster in rat testicular cells compared to human testicular cells. X rays (1 to 10 Gy) and 4-NQO (0.5 to 2.5 microM) induced ssDNA breaks to a similar extent in both human and rat testicular cells as measured by single cell get electrophoresis (SCGE) and alkaline filter elution. In contrast, 1,2-dibromo-3-chloropropane (DBCP) (3 to 300 microM) caused no significant DNA damage in human testicular cells, whereas in rats there was a clear concentration-dependent increase in ssDNA breaks. The data show that, compared to rats, testicular cells from humans are less efficient in activating DBCP to metabolites binding covalently to macromolecules. However, from the rate of covalent binding observed one would expect a significant degree of DBCP-induced ssDNA breaks in the human testicular cells. The low level of DBCP-induced ssDNA breaks in human testicular cells could indicate that different reactive DBCP metabolites are involved in binding to cellular macromolecules compared to DNA damage, or that different rates of DNA repair exist in human and rat testicular cells.
Bjørge
C
C
Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway.
Wiger
R
R
Holme
J A
JA
Brunborg
G
G
Scholz
T
T
Dybing
E
E
Søderlund
E J
EJ
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000328:Adult; D000368:Aged; D000818:Animals; D001665:Binding Sites; D001711:Biotransformation; D002273:Carcinogens / Q000633:toxicity*; D004249:DNA Damage; D004586:Electrophoresis; D005434:Flow Cytometry; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D011407:Propane / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D014965:X-Rays / Q000009:adverse effects
D002273:Carcinogens; D007306:Insecticides; D015112:4-Nitroquinoline-1-oxide; C007318:1,2-dibromo-3-chloropropane; D011407:Propane