title: Chronic constriction model of rat sciatic nerve: nerve blood flow, morphologic and biochemical alterations.
abstract: We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.
authors.affiliation: Department of Neurology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Kihara
authors.forename: P J
authors.initials: PJ
authors.lastname: Zollman
authors.forename: K K
authors.initials: KK
authors.lastname: Nickander
authors.forename: I L
authors.initials: IL
authors.lastname: Smithson
authors.forename: J D
authors.initials: JD
authors.lastname: Schmelzer
authors.forename: C L
authors.initials: CL
authors.lastname: Willner
authors.forename: E E
authors.initials: EE
authors.lastname: Benarroch
authors.forename: P A
authors.initials: PA
authors.lastname: Low
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003251:Constriction, Pathologic; D008297:Male; D008954:Models, Biological; D009638:Norepinephrine / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D012584:Sciatic Nerve / Q000098:blood supply* / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D009638:Norepinephrine