Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein.
The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.
Schmidt
M
M
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
Lindqvist
C
C
Salmi
A
A
Oker-Blom
C
C
D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus