title: Growth parameters and rate of expression of some CD markers in sorted and not sorted macrophage cultures.
abstract: Several parameters of in vitro culturing were investigated for two kinds of human macrophages cell fractions, i.e. sorted by flow cytometry and not sorted, as follows:--growth ability during serial in vitro passages, and--percent rate of CD13, CD14 and CD33 expression at different in vitro passage levels. Results point out the ability of sorted cells to survive to a higher number of serial passages. A dramatical loss of CD13 and especially CD33 markers was noticed during culturing within the not sorted cells, as compared to homologous sorted ones.
authors.affiliation: Faculty of Medicine Craiova, Dept. of Microbiology and Immunology, Craiova, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Voiculescu
authors.forename: C
authors.initials: C
authors.lastname: Avramescu
authors.forename: E
authors.initials: E
authors.lastname: Radu
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D018826:CD13 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology*; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D003470:Culture Media; D018950:Lipopolysaccharide Receptors; D063268:Sialic Acid Binding Ig-like Lectin 3; D018826:CD13 Antigens