title: Differentiation of vaccine virus from field isolates of feline panleukopenia virus by polymerase chain reaction and restriction fragment length polymorphism analysis.
abstract: In an attempt to distinguish feline panleukopenia virus (FPLV) live vaccine strains from FPLV field isolates in Japan, we compared restriction fragment length polymorphisms (RFLP) of polymerase chain reaction (PCR)-amplified fragments of live FPLV vaccine strains with those of FPLV Japanese field isolates. On the basis of nucleotide sequence differences between PLI-IV, a live vaccine strain, and FPV-483, a recent field isolate, two restriction enzymes, Dra I and Afa I, were selected for PCR-RFLP analysis of nucleotide (nt) differences at nt 3695 and 4508, respectively. Three live vaccine strains including the PLI-IV strain could be distinguished from the Japanese field isolates by their PCR-RFLP patterns by Afa I, but one live vaccine strain was indistinguishable from the Japanese isolates when Dra I and Afa I were used. The Japanese field isolates were divided into two groups by the profile of PCR-RFLP patterns generated by Dra I and Afa I, suggesting that PCR-RFLP analysis using several enzymes provides a good genetic estimate of strain differentiation. No isolate that shows a Dra I-negative/Afa I-negative pattern has emerged in Japan, indicating the possibility that the live vaccine viruses with a Dra I-negative/Afa I-negative pattern, such as the PLI-Iv strain, are candidates for use as live FPLV vaccine strain in Japan where they can be genetically distinguished from field strains.
authors.affiliation: Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, Japan. horiuchi@obihiro.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Horiuchi
authors.forename: K
authors.initials: K
authors.lastname: Yuri
authors.forename: T
authors.initials: T
authors.lastname: Soma
authors.forename: H
authors.initials: H
authors.lastname: Katae
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: M
authors.initials: M
authors.lastname: Shinagawa
mesh_terms: D000818:Animals; D002415:Cats; D017931:DNA Primers; D005243:Feces / Q000821:virology; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000821:virology*; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D017931:DNA Primers; D014765:Viral Vaccines