title: DNA-ploidy studies on cytological preparations from breast cancers by image analysis: comparison with Feulgen staining performed in destained Papanicolaou slides.
abstract: Image cytometric analysis of DNA ploidy status of 40 fresh primary breast carcinomas was performed in a comparative study on cytologic preparations stained directly by the Feulgen method and smears from the same tumors Feulgen-stained after Papanicolaou staining. There was a 0.82 overall concordance in diploid and aneuploid tumor classification and a good correlation (r = 0.736, P < 0.001) between the DNA indices determined by the two methods. Discordances are probably due to tumor heterogeneity. This study demonstrates that image cytometric DNA analysis on previously routine-stained cytologic preparations is feasible and reliable for pretreatment DNA analysis and suggests potential applications in retrospective studies with cytologic material from archives.
authors.affiliation: Institute of Pathologic Anatomy and Histology, University of Perugia, School of Medicine, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sidoni
authors.forename: A
authors.initials: A
authors.lastname: Cavaliere
authors.forename: P
authors.initials: P
authors.lastname: Alunno
authors.forename: E
authors.initials: E
authors.lastname: Bucciarelli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004396:Coloring Agents / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008875:Middle Aged; D065006:Papanicolaou Test; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*; D014626:Vaginal Smears
chemical_list: D004396:Coloring Agents; D004273:DNA, Neoplasm; C007722:Feulgen stain; D012394:Rosaniline Dyes