title: Arginine-modulated receptor-activated calcium influx via a NO/cyclic GMP pathway in human SK-N-SH neuroblastoma cells.
abstract: The effects of arginine on calcium mobilization in human SK-N-SH neuroblastoma cells were examined. It was found that arginine potentiated an increase in carbachol-induced Ca2+ from the external Ca2+ influx as opposed to an internal Ca2+ release from intracellular pools. The potentiation effect of arginine on carbachol-induced calcium mobilization was mimicked by either 8-bromo cyclic GMP or sodium nitroprusside. In addition, it was found that arginine induced NO production and an increase in cyclic GMP. Moreover, arginine-induced potentiation, NO production, and cyclic GMP increases were all suppressed after the preincubation of cells with N-methyl-L-arginine or N-nitro-L-arginine, nitric oxide synthase inhibitor. It is suggested that the NO production and subsequent cyclic GMP elevation induced by arginine are responsible for the potentiation of carbachol-induced Ca2+ increase. Our results show the existence of a NO/cyclic GMP pathway and an interconnection of NO and Ca2+ signaling pathways in human SK-N-SH neuroblastoma cells. We also observed that NO, which is produced by endothelial CPAE cells, has a modulating effect on cyclic GMP elevation in human SK-N-SH neuroblastoma cells. The intercellular communication role of NO and its cell-diffusing character may also affect the regulation of nonneuronal cells in their interactions with neuronal cells.
authors.affiliation: Department of Microbiology, Soochow University, Shihlin, Taipei, Taiwan, Republic of China.
authors.forename: P S
authors.initials: PS
authors.lastname: Liu
authors.forename: Y H
authors.initials: YH
authors.lastname: Shaw
mesh_terms: D001120:Arginine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism*; D004058:Diffusion; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D018721:Muscarinic Agonists / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism*; D009994:Osmolar Concentration; D011976:Receptors, Muscarinic / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D018721:Muscarinic Agonists; D011976:Receptors, Muscarinic; D009569:Nitric Oxide; D002217:Carbachol; D001120:Arginine; D006152:Cyclic GMP; D002118:Calcium