title: Upregulation of cortactin expression during the maturation of megakaryocytes.
abstract: Cortactin is a potent filamentous actin-binding protein acting as a prominent substrate of Src tyrosine kinases. We have evaluated cortactin expression in a series of murine tissues and shown an abundant expression of cortactin in megakaryocytes and platelets. Cortactin, but not its related protein HS1, is upregulated during the phorbol 12-myristate 13-acetate (PMA)-mediated maturation of a human megakaryoblastic cell line CMK. Although the expression of Src-related kinases is also upregulated more rapidly than cortactin in PMA-treated CMK cells, tyrosine phosphorylation of cortactin appears to be only transiently elevated 4 days after PMA stimulation. In addition, cortactin expression is induced by thrombopoietin and interleukin-3 in megakaryocytes derived from murine bone marrow cells. Thus, cortactin represents a megakaryocyte-specific gene in bone marrow and the interaction of Src kinases with cortactin may be involved in the maturation of megakaryocytes.
authors.affiliation: Department of Experimental Pathology, Jeremy H. Holland Laboratory, American Red Cross, Rockville, MD 20855, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhan
authors.forename: C C
authors.initials: CC
authors.lastname: Haudenschild
authors.forename: Y
authors.initials: Y
authors.lastname: Ni
authors.forename: E
authors.initials: E
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D051356:Cortactin; D006801:Humans; D007150:Immunohistochemistry; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation; D019061:src-Family Kinases
chemical_list: C495868:CTTN protein, human; D051356:Cortactin; C495869:Cttn protein, mouse; D008840:Microfilament Proteins; D019061:src-Family Kinases