title: Altered gene expression of the N-methyl-D-aspartate receptor channel subunits in Purkinje cells of the staggerer mutant mouse.
abstract: The gene expression of five NMDA receptor channel subunits, the epsilon(1), epsilon(2), epsilon(3), epsilon(4) and zeta(1) subunits, was examined in cerebellar Purkinje cells of the staggerer mouse at postnatal day 21. In the midline region of the staggerer cerebellum, signals for the epsilon(1), epsilon(4) and zeta(1) subunit mRNAs were distributed in Purkinje cells, which have a large cell body aligned in a monolayer between the granular and molecular layers. In addition to the midline region, labelled neurons in the intermediate cerebellar region were, though at lower levels, aligned almost in a monolayer between the granular and molecular layers. In the hemisphere, most labelled neurons occurred in various locations in the granular layer and the cerebellar medulla. These regions, populated with Purkinje cells expressing the epsilon(1), epsilon(4) and zeta(1) subunit mRNAs, were separated from each other by narrow gap regions that contained neurons without any detectable NMDA receptor channel subunit mRNAs. These results suggest that there is discrete mediolateral heterogeneity in staggerer Purkinje cell populations, in terms of expression properties of the NMDA receptor channel subunits. When compared with wild-type Purkinje cells that express the zeta(1) subunit alone, additional expression of the epsilon subunits presumably explains the persistence of NMDA responses in adult staggerer Purkinje cells (Dupont et al., Neuroscience, 12, 613-619, 1984).
authors.affiliation: Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D000818:Animals; D064026:Calbindins; D015870:Gene Expression; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants / Q000235:genetics*; D011689:Purkinje Cells / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D012016:Reference Values; D064030:S100 Calcium Binding Protein G / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D064026:Calbindins; D007473:Ion Channels; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D064030:S100 Calcium Binding Protein G