Monoclonal antibody O10 defines a conformationally sensitive cell-surface epitope of proteolipid protein (PLP): evidence that PLP misfolding underlies dysmyelination in mutant mice.
Mutations in the gene for proteolipid protein (PLP) have been associated with CNS dysmyelination and abnormal oligodendrocyte death in spontaneous mouse mutants and in Pelizaeus-Merzbacher disease; however, the effect of mutations on PLP structure and function are little understood. We have identified a monoclonal antibody directed against a novel cell surface epitope of PLP, termed O10. By immunofluorescence analysis, COS-7 cells transiently transfected to express PLP (or its isoform DM20) can be stained with antibody O10 and another antibody (A431) directed against the C terminus of PLP/DM20. The subcellular distribution of immunofluorescence labels for the two antibodies is not identical, suggesting that the O10 epitope is acquired post-translationally. When PLP/DM20 from jimpy, jimpymsd, and rumpshaker mutant mice is expressed in COS-7 cells and compared with wild-type PLP/DM20, none of the mutant isoforms displays the O10 epitope, whereas the C-terminal epitope is detected. Because the O10 but not the A431 epitope is also sensitive to SDS and reducing agents, this strongly suggests abnormal protein folding in the PLP mutants. PLP from jimpymsd mice is obviously misfolded, because the amino acid substitution (Ala242 --> Val) is located within a transmembrane domain to which the O10 antibody does not bind. We propose that the O10 epitope emerges as the full length protein reaches a functional tertiary structure and that the absence of this epitope marks a structural defect of PLP that leads to dysmyelination.
Jung
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M
Zentrum für Molekulare Biologie (ZMBH), Universität Heidelberg, D-69120 Heidelberg, Germany.
Sommer
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Schachner
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Nave
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001059:Apoproteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D000939:Epitopes; D007158:Immunologic Techniques; D051379:Mice; D008818:Mice, Neurologic Mutants; D018991:Myelin Proteolipid Protein / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D009186:Myelin Sheath / Q000502:physiology*; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational
D000911:Antibodies, Monoclonal; D001059:Apoproteins; D000939:Epitopes; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C047516:Plp1 protein, mouse