High-affinity binding of [3H]neuropeptide Y to a polypeptide from the venom of Conus anemone.
Venom preparation from Conus anemone contains a component that binds radiolabeled neuropeptide Y ([3H]neuropeptide Y) with high affinity (KD = 2.9 nM +/- 0.2 nM, Bmax = 15.2 +/- 0.5 pmol/mg protein). Binding of [3H]neuropeptide Y to the venom component is displaced with nanomolar affinity of unlabeled human and porcine neuropeptide Y, porcine [Leu31-Pro34]neuropeptide Y, peptide YY, avian and bovine pancreatic polypeptide, and the (18-36) and (25-36) C-terminal fragments from neuropeptide Y. No displacement is found with the (1-24) N-terminal neuropeptide Y fragment, human secretin, porcine dynorphin A and Boc-DAKLI (bolton Hunter coupled dynorphin A analog kappa ligand) nor with the non-peptide neuropeptide Y receptor antagonist BIBP3266. Gel filtration chromatography and denaturing (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) show that the [3H]neuropeptide Y-binding component is very likely a single-chain polypeptide with a molecular mass of 18.5 kDa.
Czerwiec
E
E
Department of Protein Chemistry, Free University of Brussels (VUB), Belgium.
De Backer
J P
JP
Vauquelin
G
G
Vanderheyden
P M
PM
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002540:Cerebral Cortex / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009478:Neuropeptide Y / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D014688:Venoms / Q000378:metabolism / Q000494:pharmacology*
D009478:Neuropeptide Y; D010455:Peptides; D014688:Venoms