Method for assessment of vascular reactivity in bone: in vitro studies on resistance arteries isolated from porcine cancellous bone.
Knowledge about vascular regulation in bone is central to the understanding of both normal and pathological bone physiology. This article describes a new method for direct assessment of the reactivity of bone blood vessels. Resistance arteries (diameter approximately 250 microns) were isolated from epiphyseal cancellous bone (porcine femoral condyle). Arterial segments (2 mm long) were mounted as ring preparations on a myograph, and isometric force development was measured continuously. Fifty-nine vessels from 31 pigs were investigated. The active force development was maximal at 0.9 x L100 in nine of 12 investigated arteries (L100 corresponds to the circumference the vessel would have if relaxed and exposed to a luminal pressure of 100 mm Hg [13.3 kPa]). In all subsequent experiments, the vessels were stretched to 0.9 x L100. Noradrenaline (2 x 10(-8) to 10(-5) M) induced a concentration-dependent vasoconstriction; mean maximal tension development was 3.69 N/m. This force development would enable the arteries to contract against a pressure of more than 22 kPa (165 mm Hg), indicating preserved function of the media smooth muscle. Response to acetylcholine (10(-7) to 10(-5) M) was observed in only two of 12 arteries. Bradykinin (10(-11) to 10(-6) M) induced a concentration-dependent and reproducible relaxation in all vessels; the relaxation was endothelium-dependent, since no effect of bradykinin was detected after mechanical removal of the endothelium. Sodium nitroprusside (10(-4) M) induced a reproducible and endothelium-independent vasorelaxation. The results demonstrate preserved function of both smooth muscle and endothelium in this preparation. The model allows pharmacological investigations of bone arteries under well defined conditions and enables studies on focal bone lesions and human bone tissue.
Lundgaard
A
A
Institute of Pharmacology, University of Aarhus, Denmark.
Aalkjaer
C
C
Holm-Nielsen
P
P
Mulvany
M J
MJ
Hansen
E S
ES
D000818:Animals; D001158:Arteries / Q000502:physiology / Q000648:ultrastructure; D001842:Bone and Bones / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D009213:Myography / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D014655:Vascular Resistance; D014661:Vasoconstriction; D014666:Vasomotor System / Q000502:physiology*