Use of a recombinant antigen, SAG2, expressed as a glutathione-S-transferase fusion protein to immunize mice against Toxoplasma gondii.
The capacity of Toxoplasma gondii surface protein SAG2 to induce protective immunity against the parasite in mice was studied using recombinant SAG2 expressed as a glutathione-S-transferase (GST) fusion protein incorporated into immune stimulating complexes (iscoms). Immunization with the iscoms resulted in the production of antibodies recognizing SAG2 as well as GST. After oral challenge infection with T. gondii oocysts or tissue cysts, no protective effect was observed. On the contrary, mice immunized with fusion SAG2 or with GST iscoms died earlier than non-immunized control mice.
Lundén
A
A
Department of Parasitology, National Veterinary Institute, Uppsala, Sweden. Anna.Lunden@sva.se
Parmley
S F
SF
Bengtsson
K L
KL
Araujo
F G
FG
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000627:therapeutic use*; D000954:Antigens, Surface / Q000627:therapeutic use*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics; D007114:Immunization; D051379:Mice; D015800:Protozoan Proteins; D016052:Protozoan Vaccines / Q000627:therapeutic use*; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use; D016019:Survival Analysis; D014124:Toxoplasmosis, Animal / Q000401:mortality / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000627:therapeutic use
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C067547:surface antigen P22, Toxoplasma; D005982:Glutathione Transferase