Comparable growth of a Trichomonas vaginalis strain in PEHPS and TYI-S-33 media.
PEHPS medium, developed for axenic cultivation of Entamoeba histolytica and E. invadens, was also capable of supporting the growth of a Trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. The logarithmic growth phase in PEHPS or in TYI-S-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in PEHPS medium showed no significant differences with those obtained in TYI-S-33 under similar culture conditions. Accordingly, PEHPS medium might be used for the axenic cultivation of T. vaginalis.
Castro-Garza
J
J
División de Biología Celular y Molecular, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México. jcastro@academ01.mty.itesm.mx
Anaya-Velázquez
F
F
Said-Fernández
S
S
González-Garza
M T
MT
D000328:Adult; D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D014246:Trichomonas vaginalis / Q000254:growth & development*; D016585:Vaginosis, Bacterial / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media