The influence of antigen-presenting cell type and interferon-gamma on priming and cytokine secretion of Leishmania major-specific T cells.
A Leishmania major-specific primary in vitro system that mimics the immune response of infected mice was used to determine the role that dendritic cells, B cells, and macrophages play in L. major T cell priming. Their relative priming potential (in order) was dendritic cells, B cells, and macrophages. Initiating primary in vitro responses with cell populations depleted of either B or dendritic cells modestly enhanced interferon (IFN)-gamma production; deleting both cells markedly enhanced IFN-gamma production. Thus, macrophages were the most effective cell for eliciting L. major Th1 cells. The effects of exogenously added IFN-gamma or neutralizing anti-IFN-gamma were also studied. With cells from genetically susceptible BALB/c mice, IFN-gamma inhibited proliferation and interleukin-4 secretion by T cells, whereas with resistant C57BL/6 cells, IFN-gamma enhanced IFN-gamma secretion. These results could not be explained by differences in IFN-gamma receptor expression.
Shankar
A H
AH
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts, USA.
Titus
R G
RG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology; D004198:Disease Susceptibility; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017471:Receptors, Interferon / Q000378:metabolism; D011994:Recombinant Proteins; D013047:Specific Pathogen-Free Organisms; D000096743:Interferon gamma Receptor
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016207:Cytokines; D007371:Interferon-gamma; D015847:Interleukin-4; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D000096743:Interferon gamma Receptor