title: Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR.
abstract: A species-specific gene probe for Hyphomicrobium facilis was generated from a transposon Tn5-132 insertion mutant defective in methanol oxidation by the inverse PCR. With this probe, the abundance of H. facilis in a garden soil was determined as a subfraction of the total cultivable hyphomicrobia.
authors.affiliation: Institut für Allgemeine Mikrobiologie, Universität Kiel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Fesefeldt
authors.forename: M
authors.initials: M
authors.lastname: Poetsch
authors.forename: C G
authors.initials: CG
authors.lastname: Gliesche
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D000432:Methanol / Q000378:metabolism; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D000432:Methanol