title: Immunosuppressive cytokines (IL-10, TGF-beta) genes expression in human gastric carcinoma tissues.
abstract: BACKGROUND
  Contribution of immunosuppressive cytokines to tumor progression in many types of cancers has been suggested. To characterize the in vivo expression of immunosuppressive cytokines in gastric cancer, we analyzed the messenger RNA (mRNA) expression of transforming growth factor-beta (TGF-beta) and interleukin-10 (IL-10) in human gastric carcinoma tissues.
  
  
  METHODS
  Both tumor tissues and nontumor tissues from each resected specimen of 29 primary gastric carcinomas were tested for IL-10 and TGF-beta mRNA expression by the reverse transcriptase-polymerase chain reaction (RT-PCR), and the mRNA expression was correlated with various pathological parameters of the tumors.
  
  
  RESULTS
  Among the 29 tumors, mRNAs of TGF-beta and IL-10 were detected in 79% and 62% of tumor samples, respectively. These cytokines were detected only in 31% for TGF-beta and 17% for IL-10 in nontumor samples. Both mRNAs were frequently expressed in the poorly differentiated adenocarcinomas and the tumor tissues with high degree of stage or lymph node metastasis.
  
  
  CONCLUSIONS
  Local expression of immunosuppressive cytokines may contribute to the progression of primary gastric carcinomas possibly through immunosuppression.
authors.affiliation: First Department of Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Morisaki
authors.forename: M
authors.initials: M
authors.lastname: Katano
authors.forename: A
authors.initials: A
authors.lastname: Ikubo
authors.forename: K
authors.initials: K
authors.lastname: Anan
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Torisu
mesh_terms: D000199:Actins / Q000235:genetics; D000230:Adenocarcinoma / Q000235:genetics* / Q000276:immunology / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015870:Gene Expression; D006801:Humans; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000235:genetics*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D000199:Actins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10