Reproducibility of simplified techniques for the measurement of 51Cr-EDTA clearance.
The aim of this study was to assess the reproducibility of a number of simplified clearance methods using chromium-51 ethylenediamine tetraacetic acid (51Cr-EDTA) and to compare these with the multiple blood sample technique. Twenty-four healthy volunteers were enrolled into the study. The test was performed twice, 1 week apart and under similar physiological conditions. After intravenous injection of the tracer, 13 blood samples were taken between 5 and 240 min. The reference clearance was calculated after adapting a bi-exponential fit on the whole plasma disappearance curve. Simplified methods included the slope intercept method using different combinations of blood samples (2 and 4 h; 2, 3 and 4 h; 1.5, 2, 3 and 4 h; 1.5, 2 and 3 h) and several single clearance methods (Constable 3 h; Groth 3 h; Groth 4 h). The standard deviation of the differences between the first and second measurements were similar for all methods, confirming the comparable reproducibility of these methods.
Piepsz
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Department of Radioisotopes, Hôpital Saint-Pierre, Brussels, Belgium.
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Ham
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D002860:Chromium Radioisotopes / Q000097:blood / Q000493:pharmacokinetics; D004492:Edetic Acid / Q000493:pharmacokinetics; D005069:Evaluation Studies as Topic; D006801:Humans; D008657:Metabolic Clearance Rate; D012016:Reference Values; D015203:Reproducibility of Results
D002860:Chromium Radioisotopes; D004492:Edetic Acid