Respiratory stimulation and generation of superoxide radicals in Pseudomonas aeruginosa by fungal naphthoquinones.
The mechanism of action of antimicrobial naphthoquinones from the fungus Fusarium was studied by using Pseudomonas aeruginosa. Bostricoidin, methyl ether fusarubin, and fusarubin stimulated the oxygen consumption of bacterial cells and induced cyanide-insensitive oxygen consumption. These activities of the tested compounds were also observed in bacterial membrane preparations in a dose-dependent manner. Naphthoquinones stimulated the generation of superoxide anion and hydrogen peroxide. The naphthoquinone effectively acted as the electron acceptors for bacterial diaphorase, which could explain the antibacterial activity of Fusarium naphthoquinones since electron acceptors lead to the stimulation of respiratory activity and the generation of oxygen radical species.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama 729-02, Japan.
Yokoyama
K
K
Oike
S
S
Ito
M
M
Nozaki
H
H
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology*; D005670:Fusarium / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D009285:Naphthoquinones; D009243:NAD; D013481:Superoxides; D006861:Hydrogen Peroxide