Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma.
Human immunodeficiency virus type 1 (HIV-1) pol mutations are responsible for HIV-1 resistance to current antiretroviral drugs. HIV-1 RNA extraction with QIAamp HCV kit spin columns (Qiagen, Chatsworth, Calif.) followed by reverse transcription-PCR successfully recovered a 1,008-bp pol fragment from the plasma of 31 of 34 HIV-1-infected patients that was suitable for sequencing and recombinant-virus studies. The minimum HIV-1 RNA concentration required for gene recovery was 30 to 40 copies/ml, which was similar to the minimal HIV-1 RNA concentration required when phenol-chloroform or silica beads are used for RNA extraction.
Shafer
R W
RW
Division of Infectious Diseases, Stanford University Medical Center, California, USA.
Levee
D J
DJ
Winters
M A
MA
Richmond
K L
KL
Huang
D
D
Merigan
T C
TC
D002725:Chloroform; D018076:DNA, Complementary / Q000235:genetics; D015751:Genes, pol; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D019800:Phenol; D010636:Phenols; D010949:Plasma / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide
D018076:DNA, Complementary; D010636:Phenols; D012367:RNA, Viral; D019800:Phenol; D012822:Silicon Dioxide; D002725:Chloroform