Decreased liver type I 5'-deiodinase and increased brown adipose tissue type II 5'-deiodinase activity in 2,3,7,8-tetrachlorobibenzo-p-dioxin (TCDD)-treated Long-Evans rats.
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was studied on the activity of type I 5'-deiodinase (5' DI) in liver and of type II 5'-deiodinase (5' DII) in brown adipose tissue (BAT) microsomes of Long-Evans rats. TCDD decreased the activity of liver 5' DI and increased that of BAT 5' DII. It also reduced the concentration of serum total thyroxine (T4) without affecting triiodothyronine (T3). These effects of TCDD on 5'-deiodinases and serum thyroid hormones are compatible with partial hypothyroidism. Based on our results, it is not possible to decide unequivocally whether TCDD affects thyroid hormone status and 5'-deiodination by a direct action or a feedback mechanism. However, these data are important because they demonstrate that TCDD affects thyroid status in a tissue-specific manner and that its effects on thyroid hormone metabolism are much more complex than currently assumed.
Raasmaja
A
A
Department of Medical Chemistry, University of Helsinki, Finland.
Viluksela
M
M
Rozman
K K
KK
D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D007453:Iodide Peroxidase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D013974:Thyroxine / Q000097:blood / Q000187:drug effects; D014284:Triiodothyronine / Q000187:drug effects
D000072317:Polychlorinated Dibenzodioxins; D014284:Triiodothyronine; D007453:Iodide Peroxidase; D013974:Thyroxine