Human prostatic carcinoma oncogene PTI-1 is expressed in human tumor cell lines and prostate carcinoma patient blood samples.
Rapid expression cloning and differential RNA display identifies a gene, named prostate tumor inducing gene-1 (PTI-1), that is differentially expressed in prostate cancer versus normal prostate and benign prostatic hypertrophy. PTI-1 encodes a truncated and mutated human elongation factor 1 alpha, and its 5' untranslated region (UTR) shares significant homology with the 23S rRNA gene of Mycoplasma hyopneumoniae. PCR with human genomic DNAs, using PTI-1 5' UTR-specific primers, suggests that this sequence is part of the human genome. Furthermore, reverse transcription (RT)-PCR, with one primer specific to the 5' UTR region and the other to the elongation factor 1 alpha coding region, amplifies PTI-1 transcripts from total RNA of various human tumor cell lines and blood samples from prostate carcinoma patients. RT-PCR products with the predicted size and sequence of PTI-1 are detected in RNAs from cell lines of human prostate, breast, and colon carcinomas. This RT-PCR product is shown by Southern blotting and sequence analyses to contain the junction sequence between the 5' UTR and the coding region of the PTI-1 gene. Furthermore, RT-PCR analysis indicates that the PTI-1 gene is also expressed in prostate carcinoma patient-derived blood samples. On the basis of serial dilution experiments, PTI-1 can detect 1 prostate carcinoma cell in 10(8) cells not expressing PTI-1. In this context, PTI-1 represents a sensitive marker for detecting human prostate cancer in the bloodstream. This study confirms the authenticity of the PTI-1 gene and documents its potential clinical utility as a sensitive and specific indicator of prostate cancer progression.
Sun
Y
Y
Department of Pathology, Columbia-Presbyterian Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Lin
J
J
Katz
A E
AE
Fisher
P B
PB
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005189:False Positive Reactions; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D009857:Oncogenes / Q000235:genetics*; D020648:Peptide Elongation Factor 1; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000097:blood* / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004269:DNA, Bacterial; C094814:EEF1A1 protein, human; D005819:Genetic Markers; D015513:Oncogene Proteins; D020648:Peptide Elongation Factor 1