title: Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis.
abstract: The beta-galactosidase from Escherichia coli is one of the most important enzymes in molecular biology. Here we report the cloning and sequencing of a gene encoding beta-galactosidase from Lactococcus lactis and compare the predicted amino acid sequence to that from other organisms. The beta-galactosidase from L. lactis was found to be a protein of 996 residues with 68.7% similarity to the E. coli enzyme and 65.8% similarity to the enzyme from Klebsiella pneumoniae. The lactococcal beta-galactosidase has lower similarity (approx 55%) to the enzymes from other lactic acid bacteria and no significant similarity to the beta-galactosidase enzymes from Agrobacterium radiobacter, Bacillus stearothermophilus, or Clostridium thermosulfurogenes. Expression of the lacZ gene from L. lactis was found to be higher when cells were grown in medium containing lactose than when grown in glucose, and expression was higher when cells were grown at 30 degrees C than at 35 degrees C.
authors.affiliation: Institute of Food Research, Colney, Norwich, UK. HUGH.GRIFFIN@BBSRC.AC.UK.
authors.forename: H G
authors.initials: HG
authors.lastname: Griffin
authors.forename: C A
authors.initials: CA
authors.lastname: MacCormick
authors.forename: M J
authors.initials: MJ
authors.lastname: Gasson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D013294:Lactococcus lactis / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D001616:beta-Galactosidase