title: Transient increase in DNA strand breaks in car refinishing spray painters.
abstract: OBJECTIVE
  Genotoxic risk was evaluated for spray painters possibly exposed to polyester resins and acrylic enamel-based paints in automotive body repair shops.
  
  
  METHODS
  DNA (deoxyribonucleic acid) strand breaks and alkali-labile sites were measured in peripheral mononuclear blood cells ex vivo using the alkaline elution method. Samples of venous blood were taken on Monday after a free weekend and again on Friday from 38 male and 1 female spray painters and compared with the blood samples from 36 male and 3 female referents. The elution rate of each DNA sample was standardized by dividing it by the elution rate obtained from simultaneously sampled untreated Chinese hamster V79 cells.
  
  
  RESULTS
  The spray painters showed a significantly (P < 0.001) higher mean level of strand breaks and alkali-labile sites in the Friday samples [2.05 (SE 0.17)] compared with the Monday samples [1.38 (SE 0.07)]. The Monday results of the spray painters were not distinguishable from the referents' [1.41 (SE 0.10)]. The increase in DNA damage was numerically higher, but only weakly significant (use of masks, P < 0.05) or nonsignificant (use of spray booths), when fewer safety provisions were taken.
  
  
  CONCLUSIONS
  A significant increase in DNA strand breaks and alkali-labile sites was found in spray painters after a week's work. However, DNA damage seems to be reversible. The use of modern safety equipment seems to affect DNA damage only marginally. There is an urgent need to identify the genotoxic chemicals in the occupational environment of spray painters and to develop corresponding satisfactory safety measures.
authors.affiliation: Institute of Toxicology, University of Mainz, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Fuchs
authors.forename: J G
authors.initials: JG
authors.lastname: Hengstler
authors.forename: F
authors.initials: F
authors.lastname: Hummrich
authors.forename: F
authors.initials: F
authors.lastname: Oesch
mesh_terms: D000180:Acrylic Resins / Q000009:adverse effects*; D000293:Adolescent; D000328:Adult; D000336:Aerosols; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D000818:Animals; D001336:Automobiles; D016022:Case-Control Studies; D006224:Cricetinae; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010150:Paint / Q000009:adverse effects*; D012134:Respiratory Protective Devices
chemical_list: D000180:Acrylic Resins; D000336:Aerosols; D000395:Air Pollutants, Occupational