[The role of the thrombocyte-activating factor in inhibiting Na+,K(+)-ATPase in different tissues after ischemia and reperfusion of the small intestine].
Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the thrombocyte-activating factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.
Vavilova
G L
GL
Prokopenko
O N
ON
Kharlamova
O N
ON
Iaremenko
M S
MS
Sagach
V F
VF
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004224:Diterpenes; D046934:Ginkgolides; D007421:Intestine, Small / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology*; D007511:Ischemia / Q000201:enzymology*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology; D007783:Lactones / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000187:drug effects / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015427:Reperfusion Injury / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000187:drug effects; D013997:Time Factors
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; C045856:ginkgolide B; D000254:Sodium-Potassium-Exchanging ATPase