Positive selection of T cells induced by viral delivery of neopeptides to the thymus.
The relation between an antigenic peptide that can stimulate a mature T cell and the natural peptide that promoted selection of this cell in the thymus is still unknown. An experimental system was devised to address this issue in vivo-mice expressing neopeptides in thymic stromal cells after adenovirus-mediated delivery of invariant chain-peptide fusion proteins. In this system, selection of T cells capable of responding to a given antigenic peptide could be promoted by the peptide itself, by closely related analogs lacking agonist and antagonist activity, or by ostensibly unrelated peptides. However, the precise repertoire of T cells selected was dictated by the particular neopeptide expressed.
Nakano
N
N
Institut de Génétique et de Biologie Moléculaire et Cellulaire (INSERM, CNRS, Université Louis Pasteur), 1 rue Laurent Fries, 67404 Illkirch, C.U. de Strasbourg, France.
Rooke
R
R
Benoist
C
C
Mathis
D
D
D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003429:Cross Reactions; D003574:Cytochrome c Group / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006825:Hybridomas; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011993:Recombinant Fusion Proteins; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D003574:Cytochrome c Group; D018076:DNA, Complementary; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; C045084:invariant chain