Interleukin-5 and human B lymphocytes.
Interleukin-5 was initially characterized in murine systems, where it was found to have B-lymphocyte proliferative and differentiative activity and to promote the growth of eosinophils. Recombinant production of human IL-5 was soon accomplished by using murine IL-5 cDNA to isolate the highly homologous human sequence. Although human IL-5 was also found to be an eosinophil-active growth factor, a significant biologic effect of IL-5 on human B lymphocytes was initially difficult to demonstrate. More recently, reports from our own laboratory and other laboratories have provided evidence that IL-5 does have activity on human B lymphocytes and that human B lymphocytes may even produce IL-5 in some situations, suggesting the possibility of autocrine stimulatory mechanisms. However, we and others have failed to demonstrate the known heterodimeric high-affinity IL-5 receptor on human B lymphocytes, in spite of evidence that we have accumulated suggesting that human B lymphocytes specifically bind IL-5. We review our own work and that of others in an area that will remain controversial until a human B-lymphocyte IL-5 receptor is definitively identified and characterized.
Baumann
M A
MA
Research Service, Dayton VA Medical Center and Division of Hematology/Oncology, Wright State University School of Medicine, Ohio 45428, USA.
Paul
C C
CC
D000906:Antibodies / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000378:metabolism; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics
D000906:Antibodies; D015848:Interleukin-5; D012333:RNA, Messenger; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D018123:Receptors, Interleukin; D013936:Thymidine