Mouse cells expressing human intercellular adhesion molecule-1 are susceptible to infection by coxsackievirus A21.
Competitive viral binding assays have revealed previously that coxsackievirus A21 (CAV21) and human rhinovirus 14 (HRV14) share a common cell surface receptor. More recently, intercellular adhesion molecule-1 (ICAM-1) has been identified as the cellular receptor for HRV-14. Also, anti-ICAM-1 monoclonal antibodies (MAbs) blocked infection by HRV14, CAV13, CAV18, and CAV21, suggesting that these viruses share this receptor; however, this has never been established by more direct methods. In this study we show conclusively that CAV21 binds to ICAM-1 and that MAbs directed against the N-terminal domain of the molecule inhibit this attachment. Furthermore, we show that the specific interaction between ICAM-1 and 160S CAV21 virions induces formation of 135S A particles. Finally, we show transfection of normally nonsusceptible mouse L cells with human ICAM-1 cDNA renders them susceptible to infection by CAV21.
Shafren
D R
DR
Department of Microbiology, Faculty of Medicine, The University of Newcastle, New South Wales, Australia. dshafren@medicine-dmb.newcastle.ed.au
Dorahy
D J
DJ
Greive
S J
SJ
Burns
G F
GF
Barry
R D
RD
D000818:Animals; D004770:Enterovirus / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D007739:L Cells; D051379:Mice; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D014771:Virion; D014779:Virus Replication
D011991:Receptors, Virus; D018799:Intercellular Adhesion Molecule-1