The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.
The gene encoding the actin-related protein Arp3 was first identified in the fission yeast Schizosaccharomyces pombe and is a member of an evolutionarily conserved family of actin-related proteins. Here we present several key findings that define an essential role for Arp3p in the functioning of the cortical actin cytoskeleton. First, mutants in arp3 interact specifically with profilin and actin mutants. Second, Arp3 localizes to cortical actin patches which are required for polarized cell growth. Third, the arp3 gene is required for the reorganization of the actin cytoskeleton during the cell cycle. Finally, the Arp3 protein is present in a large protein complex. We believe that this complex may mediate the cortical functions of profilin at actin patches in S. pombe.
McCollum
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Howard Hughes Medical Institute and the Department of Cell Biology, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.
Feoktistova
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A
Morphew
M
M
Balasubramanian
M
M
Gould
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KL
D051378:Actin-Related Protein 3; D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D005075:Biological Evolution; D002453:Cell Cycle; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D004926:Escherichia coli; D005800:Genes, Fungal; D008840:Microfilament Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010641:Phenotype; D051304:Profilins; D011994:Recombinant Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000502:physiology*
D051378:Actin-Related Protein 3; D000199:Actins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D051304:Profilins; D011994:Recombinant Proteins