title: Antimutagenic effects of ajoene, an organosulfur compound derived from garlic.
abstract: The antimutagenic effects of ajoene, which is an organosulfur compound derived from garlic, were investigated by the Ames test. Ajoene inhibited mutagenesis induced by both benzo[a]pyrene (B[a]P) and 4-nitro-1,2-phenylenediamine (NPD) in a dose-dependent manner. In particular, NPD-induced mutagenesis was more effectively suppressed by ajoene than the B[a]P-induced type. Furthermore, the inhibition of mutagenesis by ajoene was more effective for transition-type mutations than for the frame shift type. HPLC analysis of B[a]P metabolism in the presence of the rat liver microsomal fraction (S-9) showed that ajoene dose-dependently inhibited the metabolic activation of B[a]P. This suggests that ajoene affected the metabolic enzymes in the S-9 fraction.
authors.affiliation: Bio Development Division, Nagoya Seiraku Co., Ltd., Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: R
authors.initials: R
authors.lastname: Naganawa
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: N
authors.initials: N
authors.lastname: Iwata
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Fujino
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D001711:Biotransformation / Q000187:drug effects; D004220:Disulfides / Q000302:isolation & purification / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity; D010655:Phenylenediamines / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013454:Sulfoxides
chemical_list: D016587:Antimutagenic Agents; D004220:Disulfides; D009153:Mutagens; D010655:Phenylenediamines; D010936:Plant Extracts; D013454:Sulfoxides; D001564:Benzo(a)pyrene; C012277:1,2-diamino-4-nitrobenzene; C048980:ajoene