Involution of mouse mammary glands during whole organ culture occurs via apoptosis of epithelial tissue.
Apoptosis was measured in mammary glands during whole organ culture, to determine whether regression resulting from hormone withdrawal results in epithelial cell death as in vivo involution. Glands were evaluated for morphology and DNA degradation prior to whole organ culture, after lobulo-alveolar development and 2, 4, or 6 days after hormone withdrawal. The data indicated that mammary regression during whole organ culture mimics in vivo involution and results in part from apoptosis of epithelial tissue.
Casey
T M
TM
Department of Animal and Food Sciences, University of Vermont Burlington 05405, USA.
Chen
H
H
Plaut
K
K
Chiu
J F
JF
D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D053938:DNA Fragmentation; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology
D004967:Estrogens; D007328:Insulin; D000450:Aldosterone; D011374:Progesterone; D004815:Epidermal Growth Factor; D011388:Prolactin; D006854:Hydrocortisone