Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2.
A new propionate (prp) locus of S. typhimurium was defined by mutation, was located to minute 8 of the chromosome, and was shown to be transcribed in the clockwise direction. A plasmid carrying the wild-type prp+ locus was isolated by complementation and its initial physical characterization is presented. Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. Propionate stimulated prp transcription in a merodiploid strain containing prp+ and a prp::MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. prp transcription was reduced by a factor of 2 in strains deficient in the synthesis of the global regulatory protein FruR; fruR mutants failed to grow on propionate. Propionate blocked growth of prp mutants on medium containing succinate as carbon/energy source.
Hammelman
T A
TA
Department of Bacteriology, University of Wisconsin-Madison 53706-1567, USA.
O'Toole
G A
GA
Trzebiatowski
J R
JR
Tsang
A W
AW
Rank
D
D
Escalante-Semerena
J C
JC
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007763:Lac Operon; D009154:Mutation; D010641:Phenotype; D011422:Propionates / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014158:Transcription, Genetic
D011422:Propionates; D013386:Succinates; D019802:Succinic Acid