Pooled human immunoglobulins contain anti-idiotypes with reactivity against the SLE-associated 4B4 cross-reactive idiotype.
OBJECTIVE
To identify anti-idiotype antibody activity against a regulatory anti-Sm idiotype (4B4) in pooled human IgG (IVIG).


METHODS
ELISA were used to determine anti-idiotype antibody activity in IVIG.


RESULTS
Anti-idiotype antibody activity to 4B4 was detected in IVIG. The anti-idiotypic activity in IVIG could be inhibited by antigen, Sm, and rabbit anti-idiotypic antiserum. The idiotypic binding activity was predominately directed against the 4B4 VH region.


CONCLUSIONS
IVIG contains antibodies against a lupus-associated crossreactive idiotype. These antibodies represent Ab2 beta type antibodies. The anti-idiotypic activity in IVIG may be relevant to the efficacious use of IVIG in treating rheumatic diseases.
DeKeyser
F
F
University of Texas Health Science Center, Department of Medicine, San Antonio, USA.
DeKeyser
H
H
Kazatchkine
M D
MD
Rossi
F
F
Dang
H
H
Talal
N
N
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D016756:Immunoglobulins, Intravenous