title: Eosinophils transcribe and translate messenger RNA for inducible nitric oxide synthase.
abstract: Because of the involvement of nitric oxide (NO) in inflammatory states such as parasitic and hypersensitivity disorders and the fact that eosinophils are one of the cell types implicated, we asked whether eosinophils were able to express mRNA specific to inducible NO synthase (iNOS) and iNOS protein and to secrete nitric oxide. iNOS protein was detected on eosinophil preparations by immunocytochemistry using iNOS mAb. Expression of iNOS protein was also detected by immunoblotting in human purified eosinophils and an eosinophilic leukemia cell line, Eol-3. Nitrite production was detected in the supernatant of human eosinophils and Eol-3 cells cultured for 24 h, and was completely inhibited in the presence of the NOS inhibitor N-methylester-L-arginine. iNOS cDNA was obtained by reverse transcription-PCR. After subcloning, sequencing of the 259-bp fragment from three different human eosinophils cDNAs revealed 97% identity with macrophage/monocyte iNOS. Our studies describe for the first time the presence of iNOS on eosinophil and a putative new role for this cell in inflammatory states such as asthma and parasitic disease.
authors.affiliation: Immunology Department, Jiménez Díaz Foundation, Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: del Pozo
authors.forename: E
authors.initials: E
authors.lastname: de Arruda-Chaves
authors.forename: B
authors.initials: B
authors.lastname: de Andrés
authors.forename: B
authors.initials: B
authors.lastname: Cárdaba
authors.forename: A
authors.initials: A
authors.lastname: López-Farré
authors.forename: S
authors.initials: S
authors.lastname: Gallardo
authors.forename: I
authors.initials: I
authors.lastname: Cortegano
authors.forename: L
authors.initials: L
authors.lastname: Vidarte
authors.forename: A
authors.initials: A
authors.lastname: Jurado
authors.forename: J
authors.initials: J
authors.lastname: Sastre
authors.forename: P
authors.initials: P
authors.lastname: Palomino
authors.forename: C
authors.initials: C
authors.lastname: Lahoz
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001483:Base Sequence; D002417:Cattle; D002469:Cell Separation; D004804:Eosinophils / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase