[The inhibition of translation in vivo in Mollicutes by thiophosphate analogs of oligodeoxyribonucleotides].
It is shown that the concentration of "antisignature" phosphorothioate analogs of oligodeoxynucleotides, complementary to the region of 165 rRNA Acholeplasma laidlawii PG-8 and Mycoplasma fermentans PG-18 responsible tor binding with ribosomal protein S4 being 0.5--1 microM synthesis of proteins in vivo decreases to 70%. A model of mechanisms is suggested to block oligonucleotides of the process of in vivo translation in mollicutes by "antisignature" phosphorothioate analogs. The advantages of the use of antisense oligonucleotides complementary to functionally significant plots of 16S rRNA to inhibit the in vivo translation are discussed in comparison with oligonucleotides, 5-nontranslated regions of mRNA serving a target for them.
Egorov
O V
OV
Skripal'
I G
IG
Dubeĭ
I Ia
IIa
Fedoriak
O D
OD
Fedoriak
D M
DM
D000128:Acholeplasma laidlawii / Q000187:drug effects* / Q000378:metabolism; D002250:Carbon Radioisotopes; D003864:Depression, Chemical; D016858:Mycoplasma fermentans / Q000187:drug effects* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012329:RNA, Bacterial / Q000187:drug effects / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000187:drug effects / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology*; D013997:Time Factors
D002250:Carbon Radioisotopes; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013873:Thionucleotides; C035638:thiophosphoric acid