title: Effect of a non-steroidal anti-inflammatory drug on tissue levels of immunoreactive prostaglandin E2, immunoreactive leukotriene, and pain after periodontal surgery.
abstract: The aim of this study was to measure tissue levels of immunoreactive prostaglandin E2 (iPGE2), immunoreactive leukotriene B4 (iLTB4), and pain after periodontal surgery and to evaluate the effect of the non-steroidal anti-inflammatory drug (NSAID), ibuprofen, on these levels. Two contralateral quadrants in each of nine patients were selected to undergo separate surgical procedures, one with ibuprofen (800 mg 1 hour presurgery and 400 mg postsurgery) and one with a placebo. Intra-operatively, a custom-made microdialysis probe, with a 3,000 dalton molecular weight cut-off, was inserted beneath the soft tissue flap and a dialysate collected every 20 minutes for 4 hours after surgery. Pain perception was measured at the same time intervals using two pain scales. Dialysate samples were assayed using two enzyme immunoassays. Mean tissue levels of iPGE2 in the placebo group increased from 74 nM at 40 minutes to a peak of 261 nM at 200 minutes. Mean tissue levels of iLTB4 in the placebo group fluctuated between 0.2 and 0.6 nM. Pain levels in this group increased continuously with time, peaking at 4 hours. Mean tissue levels of iPGE2 in the ibuprofen group were significantly suppressed, exhibiting more than a 95% reduction. This was accompanied by a significant reduction in pain. Ibuprofen had no detectable effect on tissue levels of iLTB4. These data indicate that iPGE2 and iLTB4 are present at relatively high concentrations in the periodontal tissues after surgery. Since these concentrations exceed the Kd values for binding to their respective receptors, PGE2 and LTB4 may be associated with the development of postsurgical pain and inflammation. These data also indicate that ibuprofen can successfully inhibit iPGE2 production in the periodontal tissues and in this way may help reduce postoperative pain and inflammation.
authors.affiliation: Department of Diagnostic and Surgical Sciences, University of Minnesota, Minneapolis, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: O'Brien
authors.forename: M T
authors.initials: MT
authors.lastname: Roszkowski
authors.forename: L F
authors.initials: LF
authors.lastname: Wolff
authors.forename: J E
authors.initials: JE
authors.lastname: Hinrichs
authors.forename: K M
authors.initials: KM
authors.lastname: Hargreaves
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D001665:Binding Sites; D015232:Dinoprostone / Q000032:analysis* / Q000037:antagonists & inhibitors; D004311:Double-Blind Method; D005110:Extracellular Space / Q000737:chemistry; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology / Q000627:therapeutic use*; D007124:Immunoenzyme Techniques; D007975:Leukotriene B4 / Q000032:analysis*; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D010147:Pain Measurement; D010149:Postoperative Pain / Q000188:drug therapy / Q000517:prevention & control*; D010518:Periodontitis / Q000601:surgery*; D010519:Periodontium / Q000737:chemistry; D011448:Prostaglandin Antagonists / Q000627:therapeutic use*; D018709:Statistics, Nonparametric
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D011448:Prostaglandin Antagonists; D007975:Leukotriene B4; D015232:Dinoprostone; D007052:Ibuprofen