Defense response in slash pine: chitosan treatment alters the abundance of specific mRNAs.
We used differential display to identify chitosan responsive cDNAs in slash pine cell cultures. Two clones that showed increased mRNA abundance had sequence similarity to genes with roles in major plant defense responses, clone 18 to cinnamic acid 4-hydroxylase, and clone 30 to chitinase.
Mason
M E
ME
USDA Forest Service, Southern Institute of Forest Genetics, Harrison Experimental Forest, Saucier MS 39574, USA.
Davis
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002688:Chitinases / Q000096:biosynthesis* / Q000737:chemistry; D048271:Chitosan; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000737:chemistry; D017931:DNA Primers; D018076:DNA, Complementary; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D050564:Trans-Cinnamate 4-Monooxygenase
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D002686:Chitin; D048271:Chitosan; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D050564:Trans-Cinnamate 4-Monooxygenase; D002688:Chitinases