Theiler's murine encephalomyelitis virus subgroup strain-specific infection in a murine macrophage-like cell line.
We compared infection of a murine macrophage-like cell line, J774-1, with two Theiler's murine encephalomyelitis virus subgroup strains. The GDVII strain, which is highly virulent and produces acute polioencephalomyelitis in mice, did not actively replicate in J774-1 cells, although there was a significant inhibition in cellular protein synthesis. In contrast, the DA strain, which is less virulent and causes demyelination with a persistent virus infection, productively infected J774-1 cells; however, there was less virus produced than in BHK-21 cells, and there was little if any cellular protein shutoff. These in vitro data may provide some explanation for the biological activities that are observed between both subgroup strains.
Obuchi
M
M
Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Ohara
Y
Y
Takegami
T
T
Murayama
T
T
Takada
H
H
Iizuka
H
H
D000199:Actins / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D006224:Cricetinae; D008264:Macrophages / Q000166:cytology / Q000821:virology*; D051379:Mice; D012367:RNA, Viral / Q000032:analysis; D017945:Theilovirus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D014764:Viral Proteins / Q000032:analysis
D000199:Actins; D012367:RNA, Viral; D014764:Viral Proteins