title: Effects of selenium and serum on selenoprotein W in cultured L8 muscle cells.
abstract: When rat L8 muscle cells were cultured to examine the effects of serum and selenium concentration on selenoprotein W levels and glutathione peroxidase (GPX) activities, no significant differences (P > 0.05) were found in selenoprotein W levels and GPX activities during differentiation. With three different forms of selenium, selenoprotein W levels and GPX activities were shown to increase in L8 myotubes cultured in media with these selenocompounds. Selenite was utilized more efficiently than selenocysteine for both selenoprotein W and GPX activity, but selenium as selenomethionine was less available. Both the protein content and mRNA levels for selenoprotein W were affected by the selenium content of the media. Northern blot data indicated that the expression of selenoprotein W mRNA increased significantly when L8 myotubes were cultured with selenium (P > 0.05). L8 myotubes cultured in 10% calf serum (CS) versus 2% CS with or without addition of 10(-8) M selenium indicated that the increase of selenoprotein W level in L8 myotubes cultured with higher serum concentration (10% CS) is due to the higher selenium concentration in media rather than serum itself.
authors.affiliation: Department of Agricultural Chemistry, Oregon State University, Corvallis 97331, USA.
authors.forename: J Y
authors.initials: JY
authors.lastname: Yeh
authors.forename: B R
authors.initials: BR
authors.lastname: Ou
authors.forename: N E
authors.initials: NE
authors.lastname: Forsberg
authors.forename: P D
authors.initials: PD
authors.lastname: Whanger
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D005979:Glutathione Peroxidase / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000187:drug effects; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D051151:Selenoprotein W; D051140:Selenoproteins
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D051151:Selenoprotein W; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D012643:Selenium