title: A yeast artificial chromosome contig spanning the mouse immunoglobulin kappa light chain locus.
abstract: A single contig spanning the entire mouse immunoglobulin kappa light chain (Igk) locus on chromosome 6 has been established using yeast and bacterial artificial chromosome clones. Detailed mapping of the Igk locus indicates that a member of the Igk-V2 gene family, located about 3.5 megabases upstream of the Igk-J-C complex, is the most distal functional Igk-V gene. Sequence analyses of Igk-V genes and anonymous DNA segments provide indications for internal duplications at the 5' end of the Igk-V locus and identify the likely origin of Igk-V orphon gene clusters located elsewhere in the mouse genome.
authors.affiliation: Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
authors.forename: I W
authors.initials: IW
authors.lastname: Schupp
authors.forename: T
authors.initials: T
authors.lastname: Schlake
authors.forename: T
authors.initials: T
authors.lastname: Kirschbaum
authors.forename: H G
authors.initials: HG
authors.lastname: Zachau
authors.forename: T
authors.initials: T
authors.lastname: Boehm
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D006224:Cricetinae; D004247:DNA; D004251:DNA Transposable Elements; D006822:Hybrid Cells; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family
chemical_list: D004251:DNA Transposable Elements; D007145:Immunoglobulin kappa-Chains; D004247:DNA