Cloning and expression of a plant homologue of the small subunit of the Golgi-associated clathrin assembly protein AP19 from Camptotheca acuminata.
Clathrin-coated vesicles (CCVs) are involved in selective protein transport in eukaryotes. AP-1 and AP-2 are protein complexes found in the CCVs of the Golgi apparatus and the plasma membrane respectively. AP19 is the smallest polypeptide chain components of AP-1. We have identified a cDNA clone (CAP19) encoding a putative homologue for the assembly protein AP19 from the Chinese medicinal tree, Camptotheca acuminata. The deduced polypeptide contains 161 amino acids and has a predicted Mr of 18820. DNA blot analysis suggests that the AP19S of C. acuminata are encoded by a small gene family. CAP19 was expressed ubiquitously throughout the plant suggesting that it may be involved in general Golgi-mediated secretion.
Maldonado-Mendoza
I E
IE
Department of Biology, Texas A&M University, College Station 77843-3258, USA.
Nessler
C L
CL
D033961:Adaptor Protein Complex 1; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D002966:Clathrin; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D006056:Golgi Apparatus / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D033921:Vesicular Transport Proteins
C103697:AP19 protein, Camptotheca acuminata; D033961:Adaptor Protein Complex 1; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D033921:Vesicular Transport Proteins; C499071:adaptor protein complex 1, sigma 1 subunit