Chemical composition of the cell wall of lactic acid bacteria and related species.
In order to examine the relationship between biological activities and the cell wall content, the murein type and the teichoic acid of the cell wall from five strains of bacteria were studied. Two of these Lactobacillus casei CRL 431 and L. acidophilus CRL 730, are used in a commercial fermented milk (BIO MILK), which is believed to be beneficial for health. The other strains, Lactococcus lactis CRL 526, Pediococcus pentosaceus CRL 923 and Propionibacterium acidipropionici CRL 1198 were included in order to compare the cell wall structures of active and inactive strains. A method was designed to confirm the amino acids of the peptidoglycan in impure substrates. Four of the studied strains, L. casei, L. acidophilus, L. lactis and P. acidipropionici, contained glycerol teichoic acids. L. casei, L. acidophilus, P. pentosaceus and L. lactis contained A4 alpha type murein, while P. acidipropionici contained A3 gamma type. The capacity of orally administered peptidoglycans of the studied strains to stimulate phagocytosis by mouse peritoneal macrophages was analyzed. Only the PG of L. casei showed this activity. No differences were observed between active and inactive strains with respect to the chemical composition of the peptidoglycan. Therefore the biological activity is unlikely to be due to the peptidoglycan structure.
de Ambrosini
V M
VM
Centro de Referencia para Lactobacilos (CERELA), Chacabuco, Tucumán, Argentina.
Gonzalez
S
S
Perdigon
G
G
de Ruiz Holgado
A P
AP
Oliver
G
G
D000818:Animals; D002473:Cell Wall / Q000737:chemistry*; D002611:Cheese / Q000382:microbiology; D002648:Child; D005243:Feces / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry*; D007779:Lactobacillus acidophilus / Q000737:chemistry; D007780:Lacticaseibacillus casei / Q000737:chemistry; D013294:Lactococcus lactis / Q000737:chemistry; D051379:Mice; D010376:Pediococcus / Q000737:chemistry*; D010457:Peptidoglycan / Q000737:chemistry; D011424:Propionibacterium / Q000737:chemistry*; D013682:Teichoic Acids / Q000737:chemistry
D010457:Peptidoglycan; D013682:Teichoic Acids