Nitric oxide-related neural components in the rat small intestine after transplantation.
The changes in nitric oxide (NO)-related neural components in the transplanted small intestine are unknown. In this study, the NO neural component was examined using electrophysiological and NADPH-diaphorase histochemical techniques in a rat small bowel transplantation model. Syngeneic total small bowel transplantation was performed in 26 male Lewis rats using microsurgical techniques. The rats were divided into four groups: an untreated control group and animals at 1 (G1), 2 (G2), and 4 (G4) weeks after transplantation. Jejunal strips were mounted in a superfusion apparatus for examination. In the presence of atropine and guanethidine, the effect of the NO synthesis inhibitor L-NG-nitro-arginine (L-NNA, 100 microM) relaxation mediated by the nonadrenergic, noncholinergic (NANC) neural system was assessed following electrical stimulation at 2Hz. The inhibitory effect of L-NNA on relaxation was taken as an indicator of NO production. The percentage of inhibition in the control group, and in G1, G2, and G4 was 43.30% +/- 6.08% (mean +/- SE), 42.10% +/- 6.69%, 43.62 +/- 10.00%, and 52.46% +/- 6.00%, respectively. Inhibition in G4 was significantly higher than in the other groups (P < 0.01). The percentage of NADPH-diaphorase-positive fibers in the control group, G1, G2, and G4 was 25.06% +/- 4.70% (mean +/- SE), 26.27% +/- 2.17%, 24.73% +/- 2.87%, and 30.76% +/- 3.19%, respectively. Again, G4 showed a significantly higher level than the other groups (P < 0.01). We conclude that increased NO production may play a significant role in maintaining the intrinsic nervous system of the small intestine after transplantation.
Nakao
K
K
Second Department of Surgery, Hyogo College of Medicine, Japan.
Ishii
H
H
Kusunoki
M
M
Yamamura
T
T
Utsunomiya
J
J
D000818:Animals; D017615:Enteric Nervous System / Q000502:physiology*; D007421:Intestine, Small / Q000502:physiology / Q000637:transplantation*; D008297:Male; D009119:Muscle Contraction; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew
D009569:Nitric Oxide