Biophysical methods for the determination of antibody-antigen affinities.
Binding specificities against virtually any antigen can be isolated from antibody libraries displayed on filamentous phage. The determination of antibody-antigen affinity constants and binding kinetics is an important part of antibody characterization, and may be predictive of antibody performance in biotechnological applications. This article, intended as a guideline for the scientist who isolates a novel antibody and wishes to characterize its binding properties, presents the authors' view on widely used methodologies for the quantitative determination of antibody-antigen interactions.
Neri
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D
Institute of Molecular Biology and Biophysics, ETH Hoenggerberg, Zürich, Switzerland. dario@mol.biol.ethz.ch
Montigiani
S
S
Kirkham
P M
PM
D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D055592:Biophysical Phenomena; D001703:Biophysics; D001709:Biotechnology; D007700:Kinetics