Pituitary adenylate cyclase-activating polypeptide: a potent activator of human intestinal ion transport.
To investigate the effects of PACAP-27 on electrolyte transport across the isolated human intestinal mucosa, changes in short-circuit current (Isc) were measured in Ussing chamber experiments. Serosally added PACAP-27 increased Isc in a concentration-dependent manner, eliciting a similar maximal effect in both the jejunal and the colonic mucosa. Bumetanide inhibited Isc responses, indicating stimulation of Cl- secretion. The potency and efficacy of PACAP-27 were comparable to those of VIP, suggesting that both peptides activate intestinal secretion by way of a common receptor located in the basolateral membrane of the intestinal epithelium.
Fuchs
M
M
Lower Saxony Institute for Peptide Research, Hannover, Germany.
Adermann
K
K
Raab
H R
HR
Forssmann
W G
WG
Kuhn
M
M
D003106:Colon / Q000502:physiology / Q000503:physiopathology; D003424:Crohn Disease / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes / Q000378:metabolism*; D005768:Gastrointestinal Hormones; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology; D007583:Jejunum / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D045265:Natriuretic Peptides; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013779:Tetrodotoxin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
C495401:ADCYAP1 protein, human; D004573:Electrolytes; D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; C072685:guanylin; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin