title: Histology of intestinal Whipple's disease revisited. A study of 48 patients.
abstract: Whipple's disease is an infectious disorder with intestinal and extra-intestinal manifestations. We reinvestigated the intestinal histology in a series of 48 patients (10 females, 38 males; mean age 56.5 years, standard deviation of the mean +/- 11.2 years). A total of 126 biopsy samples, obtained prior to, during, and after therapy, were evaluated by light microscopy. In 43 patients (90%), histology was consistent with common descriptions, while it was uncommon in 3 patients (6%), and non-diagnostic in 2 patients (4%). During treatment, several alterations occurred. Apart from a continuous decrease in PAS-positive macrophages, the pattern of mucosal infiltration changed from diffuse to patchy. Moreover, the cytological aspects of PAS-positive macrophages changed substantially, and this change was used to propose four different subtypes. Initially, subtype 1 macrophages predominated (74%), but showed a gradual decrease within a few months of therapy. After 15 months, subtype 3 and subtype 4 macrophages predominated (< 80%). In 7 of 9 patients followed over long periods some subtype 3 or subtype 4 macrophages persisted. It is concluded that at diagnosis and during treatment the intestinal histology of Whipple's disease is heterogeneous. A few PAS-positive macrophages commonly persist at long-term follow-up. This and other features suggest the presence of a persistent immune defect.
authors.affiliation: Institute of Pathology, University of Heidelberg, Germany. Axel_von_Herbay@krzmail.krz.uni-heidelberg.de
authors.forename: A
authors.initials: A
authors.lastname: von Herbay
authors.forename: M
authors.initials: M
authors.lastname: Maiwald
authors.forename: H J
authors.initials: HJ
authors.lastname: Ditton
authors.forename: H F
authors.initials: HF
authors.lastname: Otto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D005500:Follow-Up Studies; D005872:Giardia / Q000302:isolation & purification; D006801:Humans; D007410:Intestinal Diseases / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology*; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D010503:Periodic Acid-Schiff Reaction; D012074:Remission Induction; D008061:Whipple Disease / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology*
chemical_list: D000900:Anti-Bacterial Agents