Stabilization of the B conformation in unoriented films of calf thymus DNA by NaCl: a Raman and IR study.
Unoriented films of calf thymus NaDNA with either 3.0 or 5.0 NaCl per base pair were prepared by dehydrating unstressed gels. These films were studied by Raman and ir spectroscopy. The 5.0 samples showed very strong vibrational modes characteristic of the B conformation at relative humidities (RH) as low as 30%, indicating that those samples were entirely in the B conformation. The 3.0 samples showed weaker features: some of the DNA in these samples were in the B conformation at 80% RH while the DNA is essentially in a disordered phase at 30% RH.
Kim
J S
JS
Department of Physics and Astronomy, University of Toledo, OH 43606, USA.
Lee
S A
SA
Carter
B J
BJ
Rupprecht
A
A
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012965:Sodium Chloride; D013059:Spectrum Analysis, Raman; D013950:Thymus Gland
D012965:Sodium Chloride; D004247:DNA