Characterization of the bovine kappa-casein gene promoter.
kappa-Casein gene promoter was localized within a 570-bp fragment (-552/+18) of a 5'-flanking region by the gene transfection assay. Deletion mutation analysis in mammary epithelial cell line, HC11, suggested that there are regulatory element in a region from -439 through -125. Some nuclear proteins from lactating rate mammary gland bind to this region specifically. One of them expressed preferentially during pregnancy bound to a 132-bp fragment (-439/-308) and another expressed preferentially during lactation bound to a 183-bp fragment (-307/-125).
Adachi
T
T
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan. 145231a@nucc.cc.nagoya-u.ac.jp
Ahn
J Y
JY
Yamamoto
K
K
Aoki
N
N
Nakamura
R
R
Matsuda
T
T
D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016385:TATA Box; D014162:Transfection
D002364:Caseins; D004268:DNA-Binding Proteins