Comparison between two HNK-1-related antibodies immunoreactivity (HNK-1-anti-leu 7 and anti-HNK-1/N-CAM) during rat cephalogenesis.
The fixation sites of two antibodies, HNK-1-anti-leu 7 and anti- HNK-1/N-CAM, were studied during visceral cephalogenesis in the rat. The labelling patterns of both antibodies were different. Anti-leu 7 staining was restricted to trunk neural crest cells, the peripheral nervous system, myotomes and premuscular cells, the eye rudiment and the olfactory vesicle. On the other hand, anti- HNK-1/N-CAM extended to the otocyst, several epithelia (including tooth germs) and precartilages. Anti-HNK-1/N-CAM staining of somites and cranial nerve ganglia was more precocious than anti-leu 7 labelling. We conclude that both antibodies, despite several common sites labelling, display quite different immunological properties, and that the expression "HNK-1" positive has no absolute significance.
Louryan
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Laboratoire d'Anatomie et d'Embryologie Humaines, Université Libre de Bruxelles, Belgium.
Werry-Huet
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Van Sint Jan
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D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D018959:CD57 Antigens / Q000032:analysis / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000276:immunology*; D003391:Cranial Nerves / Q000737:chemistry / Q000196:embryology; D005260:Female; D006257:Head / Q000196:embryology*; D007150:Immunohistochemistry; D009132:Muscles / Q000737:chemistry / Q000196:embryology; D009432:Neural Crest / Q000737:chemistry / Q000196:embryology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000737:chemistry / Q000196:embryology
D000911:Antibodies, Monoclonal; D018959:CD57 Antigens; D015816:Cell Adhesion Molecules, Neuronal