A new method for permeabilization of the plasma membrane of cultured mammalian cells. VII. Permeabilization of growing and resting cells by vortex-stirring with high molecular weight polyacrylic acid.
The permeability of murine leukemia L1210 cells toward non-permeant fluorescent Lucifer Yellow (LY) was evaluated by flow cytometry following vortex-stirring of cells with LY in the presence of high molecular weight polyacrylic acid (A-119). Permeabilization by this technique was greatly enhanced in growth phase cells, whereas it was appreciably reduced in resting cells which had been maintained in unchanged culture medium for 2 to 4d. This reduction in permeabilization of resting cells gradually disappeared when resting cells were re-suspended in fresh culture medium, and the degree of permeabilization was restored to that of the growing cells. It is recommended that the mammalian cells used for internalization of non-permeant materials by vortex-stirring with A-119 should be in the early log phase.
Noda
Y
Y
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Shimizu
N
N
Kawazoe
Y
Y
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007546:Isoquinolines; D007939:Leukemia L1210 / Q000473:pathology; D051379:Mice; D008970:Molecular Weight
D000180:Acrylic Resins; D000970:Antineoplastic Agents; D003470:Culture Media; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D007546:Isoquinolines; C006903:carbopol 940; C017475:lucifer yellow