title: Engineering of cholera toxin A-subunit for carriage of epitopes at its amino end.
abstract: The cholera toxin A-subunit (CTA) was genetically engineered at its amino end and tested for carriage of epitopes by fusion of the STa heat-stable enterotoxin analogue CAELCCNPAC. Efficient holotoxin formation by complementation in trans with cholera toxin B-subunit (CTB) indicated no decrease in affinity for CTB but evidence of reduced toxicity suggests steric interference by the decapeptide with the active site. The holotoxin was stable, able to bind to GM1 and was recognized by anti-STa and anti-CTA antibodies. The use of a full-length CTA might have been a key step for successful genetic fusions. Based on these findings, it seems worthwhile pursue the development of CTA for construction of recombinant mucosal immunoadjuvants.
authors.affiliation: Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Mexico. jsanchez@insp3.insp.mx
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.forename: R
authors.initials: R
authors.lastname: Argotte
authors.forename: A
authors.initials: A
authors.lastname: Buelna
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002772:Cholera Toxin / Q000737:chemistry*; D004274:DNA, Recombinant; D000939:Epitopes / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D008969:Molecular Sequence Data
chemical_list: D004274:DNA, Recombinant; D000939:Epitopes; D002772:Cholera Toxin