title: The alpha 1 subunit of soluble guanylyl cyclase is expressed prenatally in the rat brain.
abstract: The mRNA encoding the alpha 1 subunit of soluble guanylyl cyclase (alpha 1sGC) was identified in a different-display screening for genes spatially and temporally regulated during the development of fetal rat brain. The initially isolated fragment of the 3' untranslated region was used for in situ hybridization and to produce full-length cDNA clones by hybridization screening of cDNA libraries and by RACE (rapid amplification of cDNA ends), respectively. In situ hybridization analysis that alpha 1sGC was absent at embryonic day 12 (E12), but by E14-E15, the forebrain exhibited dense expression in the developing striatum, medial cerebral wall containing the presumptive hippocampus, cerebellar neuroepithelium, and roof plate. Weaker expression was observed in the septum, epithalamus, ventral thalamus, pineal gland and retina. This pattern is largely maintained and refined at E18, with additional expression domains in the olfactory tubercle, nucleus accumbens, zona incerta and neocortex. During early postnatal development, the adult pattern is expressed, as previously reported. The unexpected, early expression of alpha 1sGC, in conjunction with the known absence of its heterodimeric partner, the beta subunit of sGC, from the developing rodent brain during fetal ages raises potentially novel functional roles of the alpha 1 subunit during ontogeny, and might imply the existence of an alternative beta subunit specific for the prenatal brain.
authors.affiliation: Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854, USA.
authors.forename: R
authors.initials: R
authors.lastname: Smigrodzki
authors.forename: P
authors.initials: P
authors.lastname: Levitt
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005865:Gestational Age; D006162:Guanylate Cyclase / Q000737:chemistry* / Q000235:genetics; D017403:In Situ Hybridization; D010446:Peptide Fragments / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility
chemical_list: D010446:Peptide Fragments; D006162:Guanylate Cyclase