Human antibodies responsible for binding inhibition and polymerization inhibition of human immunodeficiency virus type 1 reverse transcriptase.
Using a solid-phase non-radioisotopic (non-RI) reverse transcriptase (RT) assay, antibodies inhibiting human immunodeficiency virus type 1 (HIV-1) RT activity (RTI antibody) were investigated for their ability to inhibit binding of RT to a template-primer and DNA polymerization. The RTI antibody inhibited the binding of RT to the template-primer (BI antibody), and directly reacted with the RT-template-primer complex and inhibited enzymatic activity (PI antibody). The RTI antibody interfered with formation of the RT-template-primer complex suggesting that it recognized the antigenic site involved in template-primer binding of RT molecules. Since deoxynucleotide triphosphates (dNTPs) blocked inhibition of the RT activity by the PI antibody, the antigenic site recognized by the PI antibody may be closely related to the dNTP binding site. The seropositivities of the BI and PI antibodies were 84.6% and 91.2%, respectively, in HIV-1-infected individuals; healthy individuals, HTLV-I-positive individuals, autoimmune disease patients and leukemia patients were all seronegative. No significant correlation of residual RT activities was observed when BI and PI antibodies were compared (r = 0.688). It is possible that the epitopes recognized by the BI antibody differs from those recognized by the PI antibody. The assays described are able to detect BI and PI antibodies in the sera of HIV-1-infected individuals.
Odawara
F
F
Department of Microbiology, Osaka Medical College, Japan.
Sano
K
K
Otake
T
T
Okubo
S
S
Nakano
T
T
Takasaki
T
T
Ueba
N
N
Misaki
H
H
Nakai
M
M
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D004279:DNA, Viral / Q000096:biosynthesis; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G; D011485:Protein Binding; D012194:RNA-Directed DNA Polymerase / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins; D013698:Templates, Genetic
D004279:DNA, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase