title: Immunohistochemical localization of insulin-degrading enzyme along the rat intestine, in the human colon adenocarcinoma cell line (Caco-2), and in human ileum.
abstract: Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis 55455, USA.
authors.forename: L L
authors.initials: LL
authors.lastname: Chang
authors.forename: L E
authors.initials: LE
authors.lastname: Stout
authors.forename: W D
authors.initials: WD
authors.lastname: Wong
authors.forename: J G
authors.initials: JG
authors.lastname: Buls
authors.forename: D A
authors.initials: DA
authors.lastname: Rothenberger
authors.forename: W T
authors.initials: WT
authors.lastname: Shier
authors.forename: R L
authors.initials: RL
authors.lastname: Sorenson
authors.forename: J P
authors.initials: JP
authors.lastname: Bai
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007082:Ileum / Q000201:enzymology*; D007150:Immunohistochemistry; D007339:Insulysin / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology*; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007339:Insulysin