Tau protein after delta-9-tetrahydrocannabinol in a human neuroblastoma cell line.
1. A human neuroblastoma cell line, SH-SY5Y, was used to determine the effects of delta-9-tetrahydrocannabinol (THC) on microtubule-associated tau protein. 2. After 48-hr treatment, THC (10(-9) M) decreased 50 kD tau protein in the cytoplasmic (supernatant) fraction, and in the membrane (pellet) fraction the drug (10(-7) M) also decreased 50 kD tau protein. 3. This reduction in tau protein was accompanied by a 27% reduction (P < 0.05) in the membrane (pellet) total protein after (10(-7) M) THC and a 28% increase (P < 0.02) in cytoplasmic (supernatant) total protein after 10(-9) M THC.
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Department of Anatomy, College of Human Medicine, Michigan State University, East Lansing 48824, USA.
D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D003864:Depression, Chemical; D013759:Dronabinol / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006213:Hallucinogens / Q000494:pharmacology*; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000096:biosynthesis*
D006213:Hallucinogens; D016875:tau Proteins; D013759:Dronabinol