title: Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii.
abstract: UDP-glucose: 1,2-diacylglycerol 3-glucosyltransferase (EC 2.4.1.157), catalyzes the transfer of glucose from UDP-glucose to diacylglycerol (DAG) to yield monoglucosyldiacylglycerol (MGlcDAG) and UDP. MGlcDAG is the first glucolipid along the glucolipid pathway, and a major (nonbilayer-prone) lipid in the single membrane of Acholeplasma laidlawii. MGlcDAG is further glucosylated to give the major diglucosyldiacylglycerol (DGlc-DAG). The bilayer fractions of these lipids are crucial for the metabolic maintenance of phase equilibria close to a potential bilayer-nonbilayer transition and a nearly constant spontaneous curvature. The glucolipid syntheses are also balanced against the phosphatidylglycerol pathway, competing for the common minor precursor phosphatidic acid, to retain a constant lipid surface charge density. The 1,2-diacylglycerol 3-glucosyltransferase was purified to homogeneity from detergent-solubilized A. laidlawii cells by three column chromatography methods (enrichment approximately 9000 x), and identified as a minor 40-kDa protein by using SDS-polyacrylamide gel electrophoresis. In CHAPS detergent, mixed micelles, a cooperative dependence on anionic lipids for activity was confirmed. Dependence of the enzyme on UDP-glucose followed Michaelis-Menten kinetics while the other hydrophobic substrate dioleoylglycerol stimulated the enzyme by an activating, potentially cooperative mechanism. Physiological concentrations of the activator lipid dioleoyl-phosphatidylglycerol influenced the turnover number of the enzyme but not the interaction with UDP-glucose, as inferred from variable and constant values of the apparent Vmax and Km, respectively. Dipalmitoylglycerol was a better substrate than the oleoyl species, supporting earlier in vivo and crude enzyme data. The responses of the purified 1,2-diacylglycerol 3-glucosyltransferase indicated that (i) the regulatory features of the MGlcDAG synthesis is held by the catalytic enzyme itself, and (ii) this strongly corroborates the "homeostasis" model for lipid bilayer properties in A. laidlawii proposed earlier.
authors.affiliation: Department of Biochemistry, Umeå University, Sweden. Olof.Karlsson@chem.umu.se
authors.forename: O P
authors.initials: OP
authors.lastname: Karlsson
authors.forename: A
authors.initials: A
authors.lastname: Dahlqvist
authors.forename: S
authors.initials: S
authors.lastname: Vikström
authors.forename: A
authors.initials: A
authors.lastname: Wieslander
mesh_terms: D000128:Acholeplasma laidlawii / Q000201:enzymology*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008566:Membranes / Q000201:enzymology; D010715:Phosphatidylglycerols / Q000378:metabolism
chemical_list: D010715:Phosphatidylglycerols; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; D005964:Glucosyltransferases; C103660:1,2-diacylglycerol 3-glucosyltransferase