In vivo effect of OPC15161, a superoxide scavenger, on anti-Thy1 nephritis.
The in vivo effect of 6-(1 H-indol-3-ylmethyl)-5-methoxy-3-(2-methylpropyl)-2(1 H)-pyrazinone, 4-oxide (OPC15161), a superoxide scavenger, was studied in rats with anti-Thy1 nephritis. Rats were divided into 4 groups: G-1, normal control; G-2, anti-Thy1 nephritis; G-3 anti-Thy1 nephritis and treated with OPC15161 (50 mg/kg/day) starting at day 0; and G-4, anti-Thy1 nephritis and treated with OPC15161 starting 3 days before antibody injection. At weeks 2 and 8, rats were killed for morphological study and at week 8 for renal clearance. Results were compared among the 4 groups. OPC15161 suppressed urinary albumin/day. Total glomerular cells, mesangial cells, ED-1-positive cells/glomerulus and glomerular volume all increased and the increases were suppressed by OPC15161. Tubulointerstitial index, assessed by point counting, was improved by OPC15161 (P < 0.05 G-3, 4, vs. G-2, not significant vs. G-1). Glomerular filtration rate decreased in all nephritic animals, but the decrease in renal blood flow was less in the treated groups. These findings indicate a favorable effect of OPC15161 on the glomerular and interstitial lesions of anti-Thy1 nephritis.
Hamada
N
N
First Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Nagase
M
M
D000419:Albuminuria / Q000378:metabolism / Q000652:urine; D000818:Animals; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005921:Glomerulonephritis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000276:immunology*
D016166:Free Radical Scavengers; D011719:Pyrazines; D018800:Thy-1 Antigens; D013481:Superoxides; C067708:OPC 15161