Acetohexamide reductase activities in liver microsomes and cytosol of cisplatin-treated male rats: cisplatin indirectly modulates the microsomal enzyme activity.
Treatment with cisplatin, at a dose of 7.2 mg/kg body weight, caused a significant decrease of acetohexamide reductase activity in liver microsomes of male rats at 4 and 7 days after its treatment; this dose of cisplatin has been reported to decrease testosterone levels in serum of male rats. However, the treatment with cisplatin could not decrease acetohexamide reductase activity in liver cytosol of male rats at the days tested. Although acetohexamide reductase activity in liver microsomes of male rats, as described above, was decreased by the treatment with cisplatin, the decreased microsomal enzyme activity was nearly restored when testosterone propionate was given once daily for 7 days after cisplatin treatment. Based on these results, it is reasonable to postulate that cisplatin indirectly decreases the microsomal enzyme activity which is regulated by androgens, by causing a significant decrease of the testosterone level in serum of male rats.
Imamura
Y
Y
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Hirata
M
M
Takada
H
H
Otagiri
M
M
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002945:Cisplatin / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects* / Q000201:enzymology; D004793:Enzyme Reactivators / Q000494:pharmacology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009929:Organ Size; D051381:Rats; D011916:Rats, Inbred F344; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000494:pharmacology
D004793:Enzyme Reactivators; D013739:Testosterone; D000429:Alcohol Oxidoreductases; C064152:acetohexamide reductase; D002945:Cisplatin