title: Helicobacter pylori infection delays the healing of acetic acid-induced gastric ulcer in Japanese monkeys.
abstract: To clarify the relationship between Helicobacter pylori and the healing of gastric ulcers, we investigated the healing of acetic acid-induced gastric ulcers in the antral mucosa of Japanese monkeys (n = 5) infected with H. pylori and in control monkeys without H. pylori infection (n = 6). Using H. pylori-infected Japanese monkeys as an experimental model, gastric ulcers were induced endoscopically with acetic acid. Healing of ulcers and factors that influenced healing were studied. Continuous colonization with H. pylori was confirmed in the infected group throughout the observation period; no H. pylori were isolated from the gastric mucosa of the control group. White scarring was not observed in any infected monkeys 4 weeks after ulcer formation, but was observed in one (20%) of five monkeys at 6 weeks and in all five monkeys eight weeks after ulcer formation. In the control group, white scarring was observed in one (16.7%) of six monkeys at 4 weeks and in six monkeys at 6 (P < 0.01 vs infected group) and 8 weeks. The ammonia concentration of the gastric secretions and the grade of inflammation were significantly increased in the H. pylori-infected group compared with the control group (P < 0.01 and P < 0.001, respectively). The volume of intracellular PAS-positive substance was decreased (P < 0.025-0.01) at the ulcer margin in the infected group compared with the ulcer margin in the control group. The proliferation of gastric epithelial cells was markedly accelerated at the ulcer margin in the infected group compared with the ulcer margin in control group (P < 0.025-0.01). Our results strongly suggest that H. pylori infection delays the healing of gastric ulcers.
authors.affiliation: Second Department of Internal Medicine, Oita Medical University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kodama
authors.forename: T
authors.initials: T
authors.lastname: Fujioka
authors.forename: R
authors.initials: R
authors.lastname: Shuto
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: M
authors.initials: M
authors.lastname: Nasu
mesh_terms: D019342:Acetic Acid; D000818:Animals; D005753:Gastric Mucosa / Q000473:pathology; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D008251:Macaca; D013276:Stomach Ulcer / Q000139:chemically induced / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D014945:Wound Healing
chemical_list: D019342:Acetic Acid