Characterization of Cryptococcus neoformans by random DNA amplification.
Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.
Ruma
P
P
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.
Chen
S C
SC
Sorrell
T C
TC
Brownlee
A G
AG
D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal