title: Differential distribution of syntaxin isoforms 1A and 1B in the rat central nervous system.
abstract: Syntaxin 1 binds to several proteins of the synaptic terminal and is a central component in the pathway of protein-protein interactions that underlies docking and fusion of synaptic vesicles. Molecular studies revealed the occurrence of two isoforms, syntaxin 1A and syntaxin 1B, which coexpress in neural tissues. However, they display differential expression patterns in endocrine cell types. We generated isoform-specific antibodies that were used in Western blotting and immunocytochemical studies. First, we confirmed the sole presence of syntaxin 1A in endocrine pituitary cells. Second, we found distinctive immunolabelling patterns of each isoform in the rat olfactory system, hippocampus, striatum, thalamus and spinal cord. In addition, the principal white matter commissures displayed distinct immunoreactivity for each isoform. This report shows, for the first time, major differences between the distributions of syntaxin 1A and syntaxin 1B isoforms in the rat central nervous system.
authors.affiliation: Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Ruiz-Montasell
authors.forename: F
authors.initials: F
authors.lastname: Aguado
authors.forename: G
authors.initials: G
authors.lastname: Majó
authors.forename: E R
authors.initials: ER
authors.lastname: Chapman
authors.forename: J M
authors.initials: JM
authors.lastname: Canals
authors.forename: J
authors.initials: J
authors.lastname: Marsal
authors.forename: J
authors.initials: J
authors.lastname: Blasi
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001933:Brain Stem / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D007150:Immunohistochemistry; D007536:Isomerism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism; D016548:Prosencephalon / Q000378:metabolism; D050765:Qa-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism; D050827:Syntaxin 1; D014018:Tissue Distribution
chemical_list: D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; C494213:Stx1a protein, rat; D050827:Syntaxin 1