Chemokine expression in rat stab wound brain injury.
A traumatic injury to the adult mammalian central nervous system (CNS) results in reactive astrogliosis and the migration of hematogenous cells into the damaged neural tissue. Chemokines, a novel class of chemoattractant cytokines, are now being recognized as mediators of the inflammatory changes that occur following injury. The expression of MCP-1 (macrophage chemotactic peptide-1), a member of the beta family of chemokines, has recently been demonstrated in trauma in the rat brain (Berman et al.: J Immunol 156:3017-3023, 1996). Using a stab wound model for mechanical injury, we studied the expression of two other beta chemokines: RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) and MIP-1 beta (macrophage inflammatory protein-1 beta) in the rat brain. The stab wound injury was characterized by widespread gliosis and infiltration of hematogenous cells. Immunohistochemical staining revealed the presence of RANTES and MIP-1 beta in the injured brain. RANTES and MIP-1 beta were both diffusely expressed in the necrotic tissue and were detected as early as 1 day post-injury (dpi). Double-labeling studies showed that MIP-1 beta, but not RANTES, was expressed by reactive astrocytes near the lesion site. In addition, MIP-1 beta staining was also detected on macrophages at the site of injury. The initial expression of the chemokines closely correlated with the appearance of inflammatory cells in the injured CNS, suggesting that RANTES and MIP-1 beta may play a role in the inflammatory events of traumatic brain injury. This study also demonstrates for the first time MIP-1 beta expression in reactive astrocytes following trauma to the rat CNS.
Ghirnikar
R S
RS
Department of Pathology, VAPA Health Care System, Palo Alto, CA 94304, USA.
Lee
Y L
YL
He
T R
TR
Eng
L F
LF
D000818:Animals; D000918:Antibody Specificity; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism; D018925:Chemokines / Q000096:biosynthesis*; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism; D007150:Immunohistochemistry; D019402:Macrophage Inflammatory Proteins / Q000276:immunology / Q000378:metabolism; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014951:Wounds, Stab / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D005904:Glial Fibrillary Acidic Protein; D019402:Macrophage Inflammatory Proteins