title: The presence of posttransplant HLA-specific IgG antibodies detected by enzyme-linked immunosorbent assay correlates with specific rejection pathologies.
abstract: Posttransplant monitoring of anti-HLA antibodies with routine techniques gives unsatisfactory results due to a variety of technical limitations. We investigated how a new alternative technique correlates with posttransplant clinical events. A total of 313 nonselected serum samples from 136 patients were screened by an ELISA utilizing captured soluble HLA class I antigens. We observed the absence of anti-HLA antibody production in acute rejection cases responding to standard antirejection therapy. On the other hand, we showed a clear presence of these antibodies in acute rejection episodes not responding to standard therapy (P<0.0001) and in chronic rejection (P<0.001). We conclude that routine posttransplant monitoring by ELISA offers early risk assessment that is crucial for proper immunosuppression and for antirejection therapy choice.
authors.affiliation: Department of Clinical Medicine and Applied Biotechnology, University of Bologna, Italy.
authors.forename: A N
authors.initials: AN
authors.lastname: Costa
authors.forename: M P
authors.initials: MP
authors.lastname: Scolari
authors.forename: S
authors.initials: S
authors.lastname: Iannelli
authors.forename: A
authors.initials: A
authors.lastname: Buscaroli
authors.forename: G L
authors.initials: GL
authors.lastname: D'Arcangelo
authors.forename: B
authors.initials: B
authors.lastname: Brando
authors.forename: F
authors.initials: F
authors.lastname: Indiveri
authors.forename: M
authors.initials: M
authors.lastname: Savi
authors.forename: L C
authors.initials: LC
authors.lastname: Borgnino
authors.forename: L B
authors.initials: LB
authors.lastname: DeSanctis
authors.forename: S
authors.initials: S
authors.lastname: Stefoni
authors.forename: V
authors.initials: V
authors.lastname: Bonomini
mesh_terms: D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*
chemical_list: D006680:HLA Antigens; D007074:Immunoglobulin G