[CpG motif in DNA from immune complexes of SLE patients augments expression of intercellular adhesion molecule-1 on endothelial cells].
The sequence of 9 DNA clones obtained from DNA-anti-DNA antibody immune complexes (IC) in 11 SLE patients was analyzed and the possible pathogenic role of the circulating DNA in SLE patients was discussed. Nucleic acid length of 9 cloned DNAs ranged from 87 to 312 base pairs(bp), with a mean length of 177 +/- 62bp, which were rich in guanine (G) + cytosine(C), CpG dinucleotide and palindromic sequences. Oligonucleotide TTTTCAATTCGAAGATGATT which contain the CpG motif in hexamer palindromic sequence segments in cloned DNA augmented the expression of ICAM-1 on the endothelial cells detected by FACS analysis and also augmented the gene expression of several cytokines such as interleukin-2, interleukin-6, interleukin-8 and tumor necrosis factor alpha. These data suggest that DNA in IC of SLE patients will augment expression of ICAM-1 on endothelial cells, resulting in exacerbation of vasculitis.
Miyata
M
M
Department of Internal Medicine II, Fukushima Medical College.
Kanno
T
T
Ishida
H
H
Kobayashi
H
H
Satoh
Y
Y
Satoh
Y
Y
Nishimaki
T
T
Kasukawa
R
R
D000936:Antigen-Antibody Complex / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000209:etiology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D014657:Vasculitis / Q000209:etiology
D000936:Antigen-Antibody Complex; D009841:Oligonucleotides; D018799:Intercellular Adhesion Molecule-1; D004247:DNA