title: Differentiation of human CD34+CD38- cord blood stem cells into B cell progenitors in vitro.
abstract: Umbilical cord blood CD34+CD38- cells are a rare, quiescent, primitive progenitor subpopulation lacking expression of lymphoid and myeloid lineage specific antigens. Although myeloid, erythroid, and megakaryocytic differentiation from these cells has been described, B lineage differentiation has not been demonstrated previously. We report here that highly enriched human B cell progenitors can be consistently generated from CD34+CD38- cord blood cells using long-term culture on the murine stromal line, S17, in the absence of added cytokines. After 6-8 weeks, cell numbers increased up to 160-fold, and cultures contained > 80-90% CD10+CD19+ B progenitors. Consistent with previous reports describing delayed myeloid cell differentiation from CD34+CD38- cells, production of B cell progenitors from CD34+CD38- cord blood cells was delayed 2-4 weeks relative to cultures initiated with either CD34+CD38bright or CD34+CD38dim progenitors. Addition of Flt3 ligand to long-term cultures resulted in a 2-3-fold greater increase in the number of CD19+ cells generated after 4-8 weeks. The selective outgrowth of B cell progenitors using this culture model will be useful for a range of in vitro studies using primitive hematopoietic progenitors.
authors.affiliation: Department of Microbiology and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles 90095-1662, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Rawlings
authors.forename: S
authors.initials: S
authors.lastname: Quan
authors.forename: Q L
authors.initials: QL
authors.lastname: Hao
authors.forename: F T
authors.initials: FT
authors.lastname: Thiemann
authors.forename: M
authors.initials: M
authors.lastname: Smogorzewska
authors.forename: O N
authors.initials: ON
authors.lastname: Witte
authors.forename: G M
authors.initials: GM
authors.lastname: Crooks
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000818:Animals; D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins; D051379:Mice; D009699:N-Glycosyl Hydrolases
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; C498155:Cd38 protein, mouse; D051997:ADP-ribosyl Cyclase 1