Zymosan-activated plasma induces paw oedema by nitric oxide and prostaglandin production.
Injection of zymosan-activated plasma into the rat paw induced oedema formation. Subplantar injection of the non isoform- selective inhibitors of nitric oxide (NO) synthase, N(G)-nitro-L-Arginine methyl ester and N(G)-methyl-L-Arginine, and of a scavenger of NO, haemoglobin, inhibited the early phase of oedema development. Inhibition of cyclooxygenase activity by indomethacin reduced the late increase in paw volume after the injection of zymosan-activated plasma. Methylene blue, an inhibitor of the soluble guanylate cyclase, had no effect. Our results suggest that in paw oedema induced by zymosan-activated plasma, the inflammatory response is dependent on NO (for the early phase) and prostaglandins (for the late phase). The effect of NO is likely to be mediated by a pathway which does not involve cyclic GMP.
Cuzzocrea
S
S
Institute of Pharmacology, School of Medicine, University of Messina, Italy.
Zingarelli
B
B
Calapai
G
G
Nava
F
F
Caputi
A P
AP
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005534:Foot Diseases / Q000139:chemically induced / Q000378:metabolism; D006454:Hemoglobins / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D015054:Zymosan / Q000097:blood / Q000633:toxicity*; D019323:omega-N-Methylarginine / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D004791:Enzyme Inhibitors; D006454:Hemoglobins; D011453:Prostaglandins; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D015054:Zymosan; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin