Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus.
We had previously identified an estrogen responsive protein ULF-250, synthesized and secreted by the estrous rat uterus, which is immunologically distinct from complement C3 and alpha2-macroglobulin. The N-terminal microsequencing of ULF-250 followed by sequence homology analysis showed that this protein is a new member of a class of estrogen responsive proteins in the uterus. Polymerase chain reaction with a ULF-250 specific primer yielded partial sequence information of its message. The observed pattern of ULF-250 message in the uterus during the various stages of the reproductive cycle in the rat suggested a possible regulation of ULF-250 message by 17beta-estradiol. Upstream sequencing of ULF-250 message and its promoter domains would provide insight into the mechanism of its regulation by estradiol.
Kumar G
P
P
School of Life Sciences, Devi Ahilya University, Indore, Madhya Pradesh, India.
Laloraya
M
M
Koide
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004967:Estrogens / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000378:metabolism*
D004967:Estrogens; D011506:Proteins; C103314:estrous-specific protein, 250 kDa, rat; D004247:DNA