Indirect ultraviolet detection of biologically relevant organic acids by capillary electrophoresis.
Indirect UV detection of fourteen short-chain organic acids (e.g., oxalic acid, citric acid, malonic acid, tartaric acid, methylmalonic acid, alpha-ketoglutaric acid, succinic acid, ethylmalonic acid, methylsuccinic acid, glutaric acid, apidic acid, methylglutaric acid, lactic acid and pyruvic acid) by capillary electrophoresis is described. The method used phthalate as the UV-absorbing additive in carbonate buffer and the non-absorbing analytes were detected indirectly at 230 nm. The influences of buffer pH, ionic strength, concentration of phthalate and organic modifier on indirect signal response and migration behavior of the organic acids were investigated. Comparisons of reproducibility on migration time, limit of detection and separation efficiency among three types of capillary (e.g., polyacrylamide-coated, myristyltrimethylammonium bromide-coated and uncoated capillaries) were conducted. The method developed was applied to detect succinic acid, methylmalonic acid, citric acid, glutaric acid and lactic acid in human body fluids, and preliminary results were provided.
Chen
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Department of Chemistry, Cleveland State University, OH 44115, USA.
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Van Lente
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D002021:Buffers; D002254:Carbonates / Q000737:chemistry; D002951:Citrates / Q000097:blood* / Q000652:urine; D003998:Dicarboxylic Acids / Q000097:blood* / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006880:Hydroxy Acids / Q000097:blood* / Q000652:urine; D010795:Phthalic Acids / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014783:Viscosity
D002021:Buffers; D002254:Carbonates; D002951:Citrates; D003998:Dicarboxylic Acids; D006880:Hydroxy Acids; D010795:Phthalic Acids; C005686:sodium carbonate; C032279:phthalic acid