Expression of voltage-dependent chloride channels in the rat cochlea.
Voltage-dependent chloride (ClC) channels have not yet been identified in the cochlea. In this study, an approach utilizing the reverse transcription-polymerase chain reaction (RT-PCR) was devised to clone the cDNA of ClC channels. PCR was performed using degenerate primers corresponding to two highly conserved regions of the ClC channels. By Southern hybridization and sequencing studies, the sequences corresponding to ClC-2 and ClC-3 were found in the cochlear lateral wall, while ClC-1 was not detected. These results suggest that ClC-2 and ClC-3 might be involved in Cl- transport in the cochlear lateral wall.
Oshima
T
T
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Ikeda
K
K
Furukawa
M
M
Takasaka
T
T
D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D003051:Cochlea / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D016133:Polymerase Chain Reaction; D051381:Rats
D018118:Chloride Channels; D017931:DNA Primers; D018076:DNA, Complementary