title: Subgrouping of bovine respiratory syncytial virus strains detected in lung tissue.
abstract: Bovine respiratory syncytial virus is an important respiratory pathogen in cattle. Recently, subgroups of BRSV have been identified, based on antigenic differences. However, little is known about subgroups of BRSV that circulate in the cattle population. Therefore, we determined the reactivity of monoclonal antibodies (mAbs), directed against the G, F, or P protein of BRSV, with lung tissue from 47 calves, that suffered from severe respiratory disease. Fourteen animals (30%) proved to be infected with BRSV, because they all reacted with mAbs against the P or F protein, as detected by fluorescent antibody tests. Monoclonal antibodies against the G protein were able to discriminate between the BRSV-positive specimens: 7 strains were identified as subgroup A strains, and 5 strains as subgroup AB, which is introduced as BRSV subgroup in this paper. Two strains could not be identified unambiguously. It is concluded that BRSV subgroup A and AB were associated with severe respiratory disease, and that strains belonging to either subgroup circulated concurrently in the cattle population in the Netherlands.
authors.affiliation: Institute for Animal Science and Health (ID-DLO), Department of Mammalian Virology, Lelystad, The Netherlands.
authors.forename: R S
authors.initials: RS
authors.lastname: Schrijver
authors.forename: F
authors.initials: F
authors.lastname: Daus
authors.forename: J A
authors.initials: JA
authors.lastname: Kramps
authors.forename: J P
authors.initials: JP
authors.lastname: Langedijk
authors.forename: R
authors.initials: R
authors.lastname: Buijs
authors.forename: W G
authors.initials: WG
authors.lastname: Middel
authors.forename: G
authors.initials: G
authors.lastname: Taylor
authors.forename: J
authors.initials: J
authors.lastname: Furze
authors.forename: M W
authors.initials: MW
authors.lastname: Huyben
authors.forename: J T
authors.initials: JT
authors.lastname: van Oirschot
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases; D005455:Fluorescent Antibody Technique; D008168:Lung / Q000821:virology*; D051379:Mice; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000145:classification* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014759:Viral Envelope Proteins