title: High-resolution FISH mapping of beta-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in ffttle standard karyotypes.
abstract: High-resolution chromosomal fluorescence in situ hybridization (FISH) and R-banding allowed the chromosomal localization of the beta-defensin gene cluster (DEFB@) to chromosome band 1p12 in the river buffalo (Bubalus bubalis, 2n = 50) and to band 24q13 in sheep (Ovis aries, 2n = 54), according to standard nomenclatures. The localizations permitted the tentative assignment of the entire bovine syntenic group U25 to these chromosomes. A FISH mapping control on cattle chromosomes confirmed the localization of DEFB@ in the homoeologous chromosome and chromosome band. Comparisons were made between river buffalo chromosome 1p and the homoeologous cattle chromosome on standard karyotypes; the results are discussed in light of gene assignments, extensive chromosome band conservation among the Bovidae, and inconsistencies in domestic cattle chromosome nomenclatures.
authors.affiliation: National Research Council (CNR), IABBAM, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Iannuzzi
authors.forename: D S
authors.initials: DS
authors.lastname: Gallagher
authors.forename: G P
authors.initials: GP
authors.lastname: Di Meo
authors.forename: G
authors.initials: G
authors.lastname: Diamond
authors.forename: C L
authors.initials: CL
authors.lastname: Bevins
authors.forename: J E
authors.initials: JE
authors.lastname: Womack
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D023082:Defensins; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D005810:Multigene Family; D012756:Sheep / Q000235:genetics*; D009626:Terminology as Topic
chemical_list: D001798:Blood Proteins; D023082:Defensins; D005819:Genetic Markers