title: Changes of actin cytoskeleton during swelling and regulatory volume decrease in cultured astrocytes.
abstract: Swelling of cultured astrocytes exposed to hyposmotic medium modified the organization of the filamentous actin (F-actin) cytoskeleton, making the actin network diffuse in the cell body but concentrated at foci corresponding to the tips of the cell projections retracted by swelling. This change was reversible, and, after 2 h, the actin cytoskeleton tended to recover, and cells regained their flat and stellate shape. Cytochalasins B and D (CB and CD, respectively), which disrupt the actin cytoskeleton, did not affect regulatory volume decrease (RVD) or the swelling-activated efflux of Cl- and inositol, although 10 microM CD increased the basal efflux of taurine. The mercurial p-chloromercuribenzenesulfonate (0.5-1 mM), known to disrupt the membrane cytoskeleton in isosmotic conditions, induced a 46, 50, and 38% release of [3H]taurine, 125I, and [3H]inositol, respectively, causing cell shrinkage and retraction of the cytoskeleton. Coincidently, the swelling-stimulated release of [3H]taurine and 125I was reduced by 60 and 30%, respectively. Results of this study do not exclude the possibility that changes in the actin cytoskeleton elicited by swelling are involved in mechanisms of RVD and only indicate that the disruption caused by cytochalasins is unrelated to that process.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México DF.
authors.forename: J
authors.initials: J
authors.lastname: Morán
authors.forename: M
authors.initials: M
authors.lastname: Sabanero
authors.forename: I
authors.initials: I
authors.lastname: Meza
authors.forename: H
authors.initials: H
authors.lastname: Pasantes-Morales
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D009994:Osmolar Concentration; D051381:Rats; D013654:Taurine / Q000378:metabolism*
chemical_list: D000199:Actins; D013654:Taurine; D015638:Cytochalasin D; D003571:Cytochalasin B