Molecular cloning and analysis of the 5'-flanking region of the rat PP1 alpha gene.
We have cloned an 8 kbp genomic fragment of 5'-flanking region of the gene encoding the catalytic subunit of rat protein phosphatase 1 alpha. Neither CAAT box nor TATA box was detected but a 300 bp high GC region containing nine Sp1 transcription factor binding sites is present immediately upstream of the translation start site, demonstrating that PP1 alpha is a housekeeping gene. Luciferase reporter assay showed that transcription of PP1 alpha is controlled at the high GC region.
Nomoto
K
K
Section of Biochemistry, Hokkaido University, Japan.
Shibata
N
N
Kitamura
K
K
Mizuno
Y
Y
Kikuchi
K
K
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D054645:Protein Phosphatase 1; D051381:Rats; D015183:Restriction Mapping; D017421:Sequence Analysis; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection / Q000235:genetics
D014157:Transcription Factors; D008156:Luciferases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1