Intraotic administration of gentamicin: a new method to study ototoxicity in the crista ampullaris of the bullfrog.
A new method of local gentamicin administration was tested in the bullfrog inner ear to achieve ototoxic-induced hair cell destruction. Gelfoam pledgets soaked with known amounts of gentamicin were inserted into the perilymphatic cisterna of the bullfrog through a ventral surgical approach. A dose of 1.20 mg gentamicin, consistent with a perilymphatic concentration of 65 microg/ml, resulted in the desired ototoxic-induced hair cell damage, that is, complete hair cell destruction with minimal disruption of other components of the sensory epithelium. This study demonstrates that this is a useful and simple method to investigate the process of vestibular ototoxicity and hair cell regeneration, including aspects of hair cell destruction and repair.
Carranza
A
A
Division of Head & Neck Surgery, Victor Goodhill Ear Center, University of California at Los Angeles School of Medicine, 90095-1624, USA.
Lopez
I
I
Castellano
P
P
Hoffman
L
L
Honrubia
V
V
D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005781:Gelatin Sponge, Absorbable; D005839:Gentamicins / Q000008:administration & dosage*; D018069:Hair Cells, Vestibular / Q000187:drug effects / Q000502:physiology; D011892:Rana catesbeiana; D012038:Regeneration
D000900:Anti-Bacterial Agents; D005839:Gentamicins