Identification of novel growth-related genes linked to the mitogenic effect of PACAP on the rat pancreatic acinar cell line, AR4-2J.
The mRNA differential display technique was employed in order to identify novel growth-related genes linked to the mitogenic effect of PACAP on AR4-2J cells. Hereby, three differentially expressed and PACAP-inducible genes were found, one of these being homologous to a recently discovered putative growth-related early response gene in mice. In AR4-2J cells, mRNA levels of this novel rat gene are increased by PACAP in a rapid and transient fashion. This effect was enhanced by cycloheximide, whereas actinomycin D prevented the stimulatory effect of PACAP.
Schäfer
H
H
1st Department of Medicine, Christian-Albrechts-University of Kiel, Germany.
Trauzold
A
A
Fölsch
U R
UR
Schmidt
W E
WE
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D017931:DNA Primers; D018076:DNA, Complementary; D003609:Dactinomycin / Q000494:pharmacology; D051379:Mice; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010179:Pancreas; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
C495402:Adcyap1 protein, mouse; C495403:Adcyap1 protein, rat; D017931:DNA Primers; D018076:DNA, Complementary; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide