title: Bovine humoral immune responses to Haemonchus placei excretory secretory antigens.
abstract: The immune response of calves against excretory secretory (ES) products of adult Haemonchus placei was studied. The ES products of adult H. placei comprise at least ten polypeptides with molecular weights ranging from 10 to over 100 kDa. A primary infection of calves with H. placei did not result in a marked elevation of IgG serum antibodies reactive to ES antigens as demonstrated by ELISA. Immunoblotting experiments revealed that sera of primary infected calves recognised a 50 kDa antigen in most cases. An increase in the level of serum IgG antibodies was observed after trickle infections. The secondary immune response coincided with the recognition of a group of three ES antigens of around 50-55 kDa and one protein with a molecular weight of 24 kDa.
authors.affiliation: Utrecht University, Department of Parasitology & Tropical Veterinary Medicine, Netherlands. H.Schallig@vetmic.dgk.ruu.nl
authors.forename: H D
authors.initials: HD
authors.lastname: Schallig
authors.forename: D Z
authors.initials: DZ
authors.lastname: Moyo
authors.forename: W M
authors.initials: WM
authors.lastname: Hendrikx
authors.forename: M
authors.initials: M
authors.lastname: Eysker
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000033:anatomy & histology / Q000276:immunology* / Q000302:isolation & purification; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008970:Molecular Weight; D010270:Parasite Egg Count / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G