title: Argyrophilic nucleolar organizer regions staining is useful in predicting recurrence-free interval in oral tongue and floor of mouth squamous cell carcinoma.
abstract: BACKGROUND
  Argyrophilic nucleolar organizer regions (AgNORs) represent a tissue marker of cell proliferative activity. The purpose of this study was to assess the prognostic value of AgNORs expression in oral squamous cell carcinoma (SCC).
  
  
  METHODS
  The AgNORs area/nucleus was studied in paraffin sections by means of digital image analysis in 43 cases of stage II oral tongue and floor of the mouth SCC.
  
  
  RESULTS
  Time free of disease was considered a dependent variable of a binary indicator of AgNORs expression (7.77 microns2/nucleus as a cut-off point). High AgNORs level was associated with a statistically significant negative effect on recurrence-free interval of disease in a Cox proportional hazards models controlled for occult lymph node metastasis, involvement of the surgical margins, thickness of the lesion, and vascular invasion.
  
  
  CONCLUSIONS
  The AgNORs area increased the capability of predicting which patients have a high risk of recurrence of cancer, and its evaluation may provide useful information for the therapeutic approach to the oral tongue and floor of the mouth SCC.
authors.affiliation: Department of Head and Neck Surgery, Hospital das Clínicas, University of São Paulo, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Teixeira
authors.forename: L
authors.initials: L
authors.lastname: Antonangelo
authors.forename: L
authors.initials: L
authors.lastname: Kowalski
authors.forename: P
authors.initials: P
authors.lastname: Saldiva
authors.forename: A
authors.initials: A
authors.lastname: Ferraz
authors.forename: G
authors.initials: G
authors.lastname: Silva Filho
mesh_terms: D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D009364:Neoplasm Recurrence, Local / Q000473:pathology* / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D011379:Prognosis; D016016:Proportional Hazards Models; D016622:Silver Staining; D014062:Tongue Neoplasms / Q000473:pathology* / Q000648:ultrastructure