title: Immunomodulative effects of bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus on sheep.
abstract: Experimental bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus (BLV) were performed on sheep. BIV proviral DNA and anti-BIV antibodies were persistently detected in all BIV-inoculated sheep. A slight increase in lymphocyte counts was observed in BIV-infected sheep, but the percentages of CD4+ and CD8+ cells in sheep peripheral blood mononuclear cells (PBMCs) were not significantly changed. A transient decrease in lymphocyte blastogenic response to concanavalin. A was observed in two of three BIV-infected sheep at 3-6 months after inoculation. From 6 months after BLV-inoculation to sheep which were previously infected with BIV, the numbers of lymphocytes expressing a tumor-associated antigen (TAA) of bovine leukosis were increased compared to those of a sheep inoculated with BLV alone. The BLV titers in PBMCs and the antibody titers against BLV from sheep infected with both BIV and BLV were higher than those of a sheep inoculated with BLV alone.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hirai
authors.forename: H
authors.initials: H
authors.lastname: Kabeya
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary; D016516:CD4-CD8 Ratio; D002417:Cattle; D003208:Concanavalin A / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D016583:Enzootic Bovine Leukosis / Q000276:immunology* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D016180:Lentivirus Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D018655:Lymphocyte Count; D008297:Male; D012756:Sheep / Q000276:immunology*; D012757:Sheep Diseases / Q000276:immunology* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015295:Antigens, Tumor-Associated, Carbohydrate; D004279:DNA, Viral; D003208:Concanavalin A