[The effect of a chloride channel inhibitor and of media with a decreased concentration of chlorine ions on spontaneous oocyte maturation in the common frog and the clawed toad].
Chloride-deficient media (irrespective of anions substituted for chloride) increased the rate of spontaneous maturation of follicle-enclosed (FEOs) and denuded oocytes in Rana temporaria and Xenopus laevis. The chloride channel blocker SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid) (10 microM) significantly decreased the rate of the spontaneous maturation of the FEOs and oocytes. The addition of 10 mM (instead of 2.7 mM) calcium ions to the gluconate solution significantly increased the rate of the spontaneous maturation of FEOs of R. temporaria if it was low in the gluconate solution, and decreased it if it was close to 100%.
Skoblina
M N
MN
Kondrat'eva
O T
OT
Nikiforova
G P
GP
Huhtaniemi
I
I
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology*; D000818:Animals; D018118:Chloride Channels / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000502:physiology; D002713:Chlorine; D003470:Culture Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005942:Gluconates / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011896:Rana temporaria; D014982:Xenopus laevis
D018118:Chloride Channels; D003470:Culture Media; D005942:Gluconates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D002713:Chlorine