Growth parameters and rate of expression of some CD markers in sorted and not sorted macrophage cultures.
Several parameters of in vitro culturing were investigated for two kinds of human macrophages cell fractions, i.e. sorted by flow cytometry and not sorted, as follows:--growth ability during serial in vitro passages, and--percent rate of CD13, CD14 and CD33 expression at different in vitro passage levels. Results point out the ability of sorted cells to survive to a higher number of serial passages. A dramatical loss of CD13 and especially CD33 markers was noticed during culturing within the not sorted cells, as compared to homologous sorted ones.
Voiculescu
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C
Faculty of Medicine Craiova, Dept. of Microbiology and Immunology, Craiova, Romania.
Avramescu
C
C
Radu
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E
D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D018826:CD13 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology*; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D003470:Culture Media; D018950:Lipopolysaccharide Receptors; D063268:Sialic Acid Binding Ig-like Lectin 3; D018826:CD13 Antigens