[Biosynthesis of human calcitonin and mini-proinsulin in bacterial cells in the form of hybrid proteins with corresponding antisense peptides and a metal-binding peptide].
To verify experimentally the molecular recognition theory, plasmids were constructed that provided the efficient synthesis of hybrid proteins composed of human calcitonin or miniproinsulin, the corresponding antisense peptides, and a histidine-rich metal-binding peptide. A method for isolation of the hybrid proteins by metal-chelating chromatography, cleavage, and renaturation was developed.
Efimov
V A
VA
Buriakova
A A
AA
Fradkov
A F
AF
Chakhmakhcheva
O G
OG
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D006801:Humans; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011384:Proinsulin / Q000235:genetics*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D008670:Metals; D010455:Peptides; D011993:Recombinant Fusion Proteins; C060793:miniproinsulin; D002116:Calcitonin; D011384:Proinsulin