title: Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli.
abstract: The first demonstration of conjugal plasmid transfer from Escherichia coli to Bartonella henselae is reported. Transconjugants bearing plasmids of incompatibility groups P (IncP) and Q (IncQ), expressing various resistance markers, were generated. Tn5 transposons delivered on suicide plasmids by conjugation showed transpositional insertion into random chromosomal sites.
authors.affiliation: Unité de Pathogenie Microbienne Moléculaire, Institut Pasteur, Paris,France. dehio@mpib-tuebingen.mpg.de
authors.forename: C
authors.initials: C
authors.lastname: Dehio
authors.forename: M
authors.initials: M
authors.lastname: Meyer
mesh_terms: D018416:Bartonella henselae / Q000235:genetics; D003227:Conjugation, Genetic; D004254:DNA Nucleotidyltransferases; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D019895:Transposases
chemical_list: D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D019895:Transposases