Interleukin-4 blocks the release of collagen fragments from bovine nasal cartilage treated with cytokines.
Interleukin-1 (IL-1) in combination with other cytokines can induce a reproducible release of collagen fragments from bovine nasal cartilage in culture. Over 70% of the total collagen is released by day 14 and this release is accompanied by the appearance of collagenolytic activity in the medium that cleaves collagen specifically at the one quarter/three quarter position. Interleukin-4 is able to prevent the release of collagen fragments from the tissue and this is accompanied by a reduced secretion and activation of collagenase (MMP-1) with an increase in tissue inhibitor of metalloproteinases-1 (TIMP-1). IL-4, especially in the presence of IL-1, increased TIMP secretion by bovine nasal cartilage in culture. These results suggest that IL-4 is able to specifically block cartilage collagen resorption by down-regulating the production of collagenase (MMP-1) and up-regulating TIMP-1 by chondrocytes within the cartilage.
Cawston
T E
TE
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
Ellis
A J
AJ
Bigg
H
H
Curry
V
V
Lean
E
E
Ward
D
D
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000378:metabolism; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D061965:Matrix Metalloproteinase Inhibitors; D009300:Nasal Septum / Q000378:metabolism*; D053683:Oncostatin M; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases
D016207:Cytokines; D004792:Enzyme Precursors; D006023:Glycoproteins; D006025:Glycosaminoglycans; D061965:Matrix Metalloproteinase Inhibitors; D010446:Peptide Fragments; D010455:Peptides; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D053683:Oncostatin M; D016753:Interleukin-10; D015847:Interleukin-4; D003094:Collagen; D017364:Collagenases; C022440:procollagenase