Enhanced temporal resolution for the microdialysis monitoring of catecholamines and excitatory amino acids using capillary electrophoresis with laser-induced fluorescence detection. Analytical developments and in vitro validations.
This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of catecholamines and excitatory amino acids on submicroliter microdialysis samples, with short analysis times (3 min or less), high sensitivity (nanomolar range, i.e., attomoles detected) and high separation efficiency (up to 1.10(6) theoretical plates). A continuous flow derivatization of small volumes of microdialysate (500 nl) using naphthalene-2,3-dicarboxaldehyde as derivatizing reagent is described. Thereafter, two subsequent off-line analyses are performed on each of the 30-s dialysates to determine catecholamines and amino acids. The performances of the present method are demonstrated in vitro by monitoring rapid fluctuations in the concentration of catecholamines and amino acids in the external microdialysis medium.
Bert
L
L
Laboratoire de Neuropharmacologie et Neurochimie, INSERM, Lyon, France.
Robert
F
F
Denoroy
L
L
Stoppini
L
L
Renaud
B
B
D002395:Catecholamines / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D017551:Microdialysis; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002395:Catecholamines