Mapping of a major osteomagenic determinant of murine leukemia virus RFB-14 to non-long terminal repeat sequences.
Certain isolates of murine leukemia viruses (MuLVs) have, apart from a leukemogenic potential, the capability of inducing diseases of nonhematopoietic tissues in susceptible strains of mice. We have reported on the molecular cloning of a bone-tumorigenic virus, RFB-14 MuLV, which was found to induce benign bone tumors, osteomas, with 100% incidence in mice of the CBA/Ca strain (L. Pedersen, W. Behnisch, J. Schmidt, A. Luz, F. S. Pedersen, V. Erfle, and P. G. Strauss, J. Virol. 66:6186-6190, 1992). In order to analyze the bone tumor-inducing phenotype of RFB-14 MuLV, we have studied the pathogenic potential of recombinant viruses between RFB-14 and the nonosteomagenic, highly leukemogenic SL3-3 MuLV. The recombinants were constructed so as to reveal whether a major determinant of osteomagenicity maps to sequences within or outside the long terminal repeats (LTR). Our data show that a major determinant of the osteoma-inducing potential of RFB-14 MuLV maps to the non-LTR region of the genome. Furthermore, we demonstrate that a strong determinant of leukemogenicity is harbored by the non-LTR region of SL3-3 MuLV.
Ostergaard
M
M
Department of Molecular and Structural Biology, University of Aarhus, Denmark.
Pedersen
L
L
Schmidt
J
J
Luz
A
A
Lovmand
J
J
Erfle
V
V
Pedersen
F S
FS
Strauss
P G
PG
D016475:3T3 Cells; D000818:Animals; D002874:Chromosome Mapping; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D008223:Lymphoma / Q000821:virology; D051379:Mice; D008808:Mice, Inbred CBA; D010016:Osteoma / Q000821:virology*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid