title: Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle.
abstract: The HIR/HPC (histone regulation/histone periodic control) negative regulators play important roles in the transcription of six of the eight core histone genes during the Saccharomyces cerevisiae cell cycle. The phenotypes of hir1 and hir2 mutants suggested that the wild-type HIR1 and HIR2 genes encode transcriptional repressors that function in the absence of direct DNA binding. When Hir1p and Hir2p were artificially tethered to yeast promoters, each protein repressed transcription, suggesting that they represent a new class of transcriptional corepressors. The two proteins might function as a complex in vivo: Hir2p required both Hir1p and another Hir protein, Hir3p, to repress transcription when it was tethered to an HTA1-lacZ reporter gene, and Hir1p and Hir2p could be coimmunoprecipitated from yeast cell extracts. Tethered Hir1p also directed the periodic transcription of the HTA1 gene and repressed HTA1 transcription in response to two cell cycle regulatory signals. Thus, it represents the first example of a transcriptional corepressor with a direct role in cell cycle-regulated transcription.
authors.affiliation: Program in Molecular Biology, Sloan Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Spector
authors.forename: A
authors.initials: A
authors.lastname: Raff
authors.forename: H
authors.initials: H
authors.lastname: DeSilva
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.forename: M A
authors.initials: MA
authors.lastname: Osley
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D017930:Genes, Reporter; D006388:Hemagglutinins / Q000235:genetics; D006657:Histones / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic / Q000235:genetics; D012331:RNA, Fungal / Q000032:analysis; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology*; D001616:beta-Galactosidase / Q000235:genetics; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D006388:Hemagglutinins; D006657:Histones; D009687:Nuclear Proteins; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D001616:beta-Galactosidase; D000099160:LexA Repressor Protein; C078808:HIR1 protein, S cerevisiae; C078809:HIR2 protein, S cerevisiae