Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein.
The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein from maize and Arabidopsis ERabp1. The deduced amino acid sequence contains an endoplasmic reticulum retention signal, the KDEL sequence located at the C-terminal end, and has two possible auxin-binding sites, HRHSCE and YDDWSVPHTA conserved sequences. Northern hybridization analysis revealed that the Ca-ERabp1 gene is differentially expressed in total RNA isolated from different organs of a pepper plant, showing the highest level of expression in fruits but barely detectable in leaves and roots.
Choi
S Y
SY
Department of Microbiology, HanNam University, Taejon, Republic of Korea.
D000595:Amino Acid Sequence; D002212:Capsicum / Q000235:genetics*; D003001:Cloning, Molecular; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010946:Plants, Medicinal; D011956:Receptors, Cell Surface / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D007210:Indoleacetic Acids; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1