Ascorbate promotes low density lipoprotein oxidation in the presence of ferritin.
Whilst catalytic iron has been implicated in the development of atherosclerosis by initiating low density lipoprotein (LDL) oxidation, the source of such iron remains uncertain. Here, we show that LDL oxidation in the presence of ferritin was stimulated by ascorbate (15-60 microM), whilst this effect was inhibited by the iron chelator desferrioxamine. Ascorbate also showed an antioxidant activity at high concentrations (125-250 microM). Our results suggest that the combination of ascorbate with ferritin may supply free iron for LDL oxidation in vivo.
Dai
L
L
Inflammation Research Group, St Bartholomew's School of Medicine and Dentistry, London, UK.
Winyard
P G
PG
Zhang
Z
Z
Blake
D R
DR
Morris
C J
CJ
D001161:Arteriosclerosis / Q000503:physiopathology; D001205:Ascorbic Acid / Q000494:pharmacology*; D002614:Chelating Agents; D003676:Deferoxamine / Q000494:pharmacology; D005293:Ferritins / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000378:metabolism*
D002614:Chelating Agents; D008077:Lipoproteins, LDL; D005293:Ferritins; D007501:Iron; D003676:Deferoxamine; D001205:Ascorbic Acid