title: Two-step processing is not essential for the import and assembly of functionally active iron-sulfur protein into the cytochrome bc1 complex in Saccharomyces cerevisiae.
abstract: The iron-sulfur protein of the cytochrome bc1 complex is one of a small number of proteins that are processed in two sequential steps by matrix processing peptidase (MPP) and mitochondrial intermediate peptidase (MIP) during import into Saccharomyces cerevisiae mitochondria. To test whether two-step processing is necessary for import and assembly of the iron-sulfur protein into the cytochrome bc1 complex, we mutagenized the presequence of the iron-sulfur protein to eliminate the original MPP site and replace the MIP site with a new MPP site. The mutated presequence is cleaved and forms mature-sized protein in a single step, and the mature-sized iron-sulfur protein is correctly targeted to the outer side of the inner mitochondrial membrane in vitro. Mutant iron-sulfur protein which is processed to mature size in one step complements the respiratory deficient phenotype of a yeast strain in which the endogenous gene for the iron-sulfur protein is deleted. These results establish that mature-sized iron-sulfur protein can be formed by single-step processing and assembled into a functionally active form in the cytochrome bc1 complex in S. cerevisiae.
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Nett
authors.forename: E
authors.initials: E
authors.lastname: Denke
authors.forename: B L
authors.initials: BL
authors.lastname: Trumpower
mesh_terms: D000595:Amino Acid Sequence; D004492:Edetic Acid / Q000494:pharmacology; D014450:Electron Transport Complex III / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D010618:Phenanthrolines / Q000494:pharmacology; D010641:Phenotype; D012441:Saccharomyces cerevisiae; D000096122:Mitochondrial Processing Peptidase
chemical_list: D004492:Edetic Acid; D014450:Electron Transport Complex III; D007502:Iron Chelating Agents; D007506:Iron-Sulfur Proteins; D008666:Metalloendopeptidases; D010618:Phenanthrolines; D000096122:Mitochondrial Processing Peptidase; C068038:mitochondrial intermediate peptidase; C025205:1,10-phenanthroline