title: Relationship between DNA synthesis and growth factor expression in primary cultures of adult rat hepatocytes.
abstract: In this study we employed primary culture of adult rat hepatocytes to verify the effects of two different extracellular matrices (collagen, matrigel) on EGF-stimulated DNA synthesis and c-myc expression. Our results confirm that in adult rat hepatocytes EGF induces DNA synthesis, preceded by a transient increase of c-myc expression, when cells are cultured at low density on collagen. DNA synthesis appears to be in reciprocal relationship with hepatic expression of IGF-I, IGFBP-1, IGFBP-2 and IGFBP-4, suggesting that IGF-I/IGFBPs system is not involved in liver growth.
authors.affiliation: Istituto di Fisiologia Generale, Università di Genova.
authors.forename: A
authors.initials: A
authors.lastname: Voci
authors.forename: M
authors.initials: M
authors.lastname: Massajoli
authors.forename: C
authors.initials: C
authors.lastname: Bottazzi
authors.forename: I
authors.initials: I
authors.lastname: Demori
authors.forename: G
authors.initials: G
authors.lastname: Gallo
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D011509:Proteoglycans / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C059485:matrigel; D004815:Epidermal Growth Factor; D003094:Collagen; D004247:DNA