title: Influence of tooth cryopreservation and storage time on microleakage.
abstract: OBJECTIVE
  This study was conducted to compare microleakage of two new dentin bonding agents on freshly extracted teeth, cryopreserved teeth, or teeth stored in water containing 0.5% chloramine at 4 degrees C.
  
  
  METHODS
  Rectangular Class V cavity preparations were made on the buccal and the lingual surface of wisdom teeth. They were filled with either Scotchbond Multi-Purpose and Z100 (3M Dental Products) or with Gluma 2000 and Pekafill (Bayer Dental). After thermocycling, silver staining penetration was evaluated under a light microscope. SEM examination and EDX analysis were performed to evaluate the microleakage pattern. The results were analyzed by the use of a two-way analysis of variance.
  
  
  RESULTS
  Cryopreservation for 13 wk or 12 d refrigeration did not produce changes in the amount of microleakage. However, 48 d or longer of refrigeration increased microleakage. There was no correlation between changes in microleakage and storage time. Specimens prepared with both dentin bonding agents exhibited the same microleakage values and the same microleakage pattern.
  
  
  CONCLUSIONS
  Refrigeration at 4 degrees C in 0.5% chloramine for 48 d or longer may cause an increase in microleakage. Cryopreservation for 13 wk or short-term refrigeration did not affect the microleakage.
authors.affiliation: Faculté d'Odontologie, Unité IMEB, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Camps
authors.forename: X
authors.initials: X
authors.lastname: Baudry
authors.forename: V
authors.initials: V
authors.lastname: Bordes
authors.forename: J
authors.initials: J
authors.lastname: Dejou
authors.forename: C
authors.initials: C
authors.lastname: Pignoly
authors.forename: P
authors.initials: P
authors.lastname: Ladeque
mesh_terms: D000704:Analysis of Variance; D003188:Composite Resins; D015925:Cryopreservation; D003763:Dental Leakage / Q000175:diagnosis; D017220:Dentin-Bonding Agents; D006801:Humans; D019279:Resin Cements; D013048:Specimen Handling / Q000379:methods; D014070:Tooth; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; C092418:Gluma 2000; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; D014867:Water