title: Mapping of the active site of recombinant human erythropoietin.
abstract: Recombinant human erythropoietin (rHuEPO) variants have been constructed to identify amino acid residues important for biological activity. Immunoassays were used to determine the effect of each mutation on rHuEPO folding. With this strategy, we could distinguish between mutations that affected bioactivity directly and those that affected bioactivity because the mutation altered rHuEPO conformation. Four regions were found to be important for bioactivity: amino acids 11 to 15, 44 to 51, 100 to 108, and 147 to 151. EPO variants could be divided into two groups according to the differential effects on EPO receptor binding activity and in vitro biologic activity. This suggests that rHuEPO has two separate receptor binding sites. Mutations in basic residues reduced the biologic activity, whereas mutations in acidic residues did not. This suggests that electrostatic interactions between rHuEPO and the human EPO receptor may involve positive charges on rHuEPO.
authors.affiliation: Amgen Center, Thousand Oaks, CA 91320, USA.
authors.forename: S
authors.initials: S
authors.lastname: Elliott
authors.forename: T
authors.initials: T
authors.lastname: Lorenzini
authors.forename: D
authors.initials: D
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Barzilay
authors.forename: E
authors.initials: E
authors.lastname: Delorme
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010449:Peptide Mapping; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D004921:Erythropoietin