title: Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.
abstract: We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.
authors.affiliation: Department of Microbiology, Université Laval, Quebec City, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Boivin
authors.forename: J
authors.initials: J
authors.lastname: Handfield
authors.forename: G
authors.initials: G
authors.lastname: Murray
authors.forename: E
authors.initials: E
authors.lastname: Toma
authors.forename: R
authors.initials: R
authors.lastname: Lalonde
authors.forename: J G
authors.initials: JG
authors.lastname: Lazar
authors.forename: M G
authors.initials: MG
authors.lastname: Bergeron
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000956:Antigens, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D015658:HIV Infections / Q000821:virology; D006801:Humans; D009504:Neutrophils / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral