Molecular cloning of a cDNA encoding an antigen which is salt-stably attached to centrosomes.
A monoclonal antibody (MAB 2A8) was used for expression-cloning of a complete cDNA (1133/5) to a mRNA (3 kb) encoding a murine 76 kDa polypeptide. The N-terminal section of the polypeptide is composed of domains capable to form alpha-helical coiled-coils. Its C-terminus is proline-rich and has characteristics of the Src homology region 3 (SH3). Affinity-purified antibodies to a recombinant section of the protein show that the antigen is salt-stably associated with the centrosome throughout the cell cycle.
Angiolillo
A
A
University of Perugia, Institute of Cellular and Molecular Biology, Italy.
Batova
I
I
Joswig
G
G
Werner
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D018385:Centrosome / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017421:Sequence Analysis
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D000939:Epitopes; D010455:Peptides; D011994:Recombinant Proteins