title: Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli.
abstract: An expression plasmid carrying an alkaline lipase gene from Proteus vulgaris was constructed. The lipase content in Escherichia coli cells harboring the expression plasmid reached about 22% of total soluble protein. The purified enzyme displayed a partial interfacial activation toward p-nitrophenyl butyrate (PNPB).
authors.affiliation: Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Yusung, Taejon, South Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Park
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: T K
authors.initials: TK
authors.lastname: Oh
mesh_terms: D002087:Butyrates / Q000819:agonists*; D004926:Escherichia coli; D015870:Gene Expression; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011514:Proteus vulgaris; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D002087:Butyrates; D011994:Recombinant Proteins; C033592:4-nitrophenyl butyrate; D008049:Lipase