The influence of HLA B27 and interferon-gamma on the invasion and persistence of yersinia in primary human fibroblasts.
To examine the role of the human HLA class I molecule B27 in the direct interaction of yersinia and human cells we performed experimental infections of constitutive HLA B27-positive and -negative human fibroblast monolayers with Yersinia enterocolitica O.3. The numbers of yersiniae invading HLA B27-positive cells were within the range of HLA B27-negative cells. Treatment of fibroblasts with interferon-gamma (IFN-gamma) to enhance HLA B27 expression did not alter invasion. IFN-gamma reduced titers of live intracellular bacteria, but not bacterial antigens, in a dose-dependent manner. Persistent infection with yersinia could be maintained for up to 10 weeks with decreasing bacterial titers and more slowly decreasing amounts of yersinial antigens in HLA B27-positive and -negative cells. Endogenous HLA B27 does not modulate the direct interaction of yersiniae with primary human fibroblasts. This is in contrast to infection of HLA B27-transfected murine cells. The protective effect of IFN-gamma for yersinia-infected cells may be important for controlling complications in patients with yersiniosis.
Huppertz
H I
HI
Universitätskinderklinik, Würzburg, Germany.
Heesemann
J
J
D018829:Adhesins, Bacterial / Q000032:analysis; D002460:Cell Line; D005347:Fibroblasts / Q000382:microbiology; D015796:HLA-B27 Antigen / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D014162:Transfection; D015008:Yersinia enterocolitica / Q000502:physiology*
D018829:Adhesins, Bacterial; D015796:HLA-B27 Antigen; C064832:YadA protein, Yersinia; D007371:Interferon-gamma