title: The role of B7-1 and LFA-3 in costimulation of CD8+ T cells.
abstract: This study compares the ability of LFA-3 (CD58) and B7-1 (CD80) ligands to provide costimulatory signals for superantigen (SAg)-stimulated CD8+ and CD4+ T cells. We show that B7-1 and LFA-3 costimulation activate CD8+ T cells to proliferation, cytokine production (IL-2, TNF, and IFN-gamma), and cytotoxicity. A long-lasting proliferative response was observed after combined DR/B7-1/LFA-3 costimulation. Detailed analysis of SEA-activated CD8+ T cells revealed that maximal production of IFN-gamma was seen in LFA-3-costimulated cells, while production of IL-2 was mainly induced after B7-1 costimulation. A fivefold increase in the IFN-gamma production was observed when activated CD8+ T cells were costimulated with Chinese hamster ovary (CHO)-DR/LFA-3 cells compared with the secretion induced by CHO-DR/B7-1. In contrast, SEA-treated CD4+ T cells costimulated with B7-1 or LFA-3 gave rise to a similar production of IFN-gamma, suggesting a preferential function for the CD2/LFA-3 pathway in the regulation of IFN-gamma in CD8+ T cells. Moreover, the generation of CTL was supported similarly by B7-1 and LFA-3 costimulation, but not by CHO-DR cells. We conclude that ligation of the CD28 and CD2 receptors mediate distinct effect on CD8+ and CD4+ T cell effector functions.
authors.affiliation: The Wallenberg Laboratory, Department of Cell and Molecular Biology, University of Lund, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Parra
authors.forename: A G
authors.initials: AG
authors.lastname: Wingren
authors.forename: G
authors.initials: G
authors.lastname: Hedlund
authors.forename: T
authors.initials: T
authors.lastname: Kalland
authors.forename: M
authors.initials: M
authors.lastname: Dohlsten
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D018968:CD58 Antigens / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D004357:Drug Synergism; D004768:Enterotoxins / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D018089:Superantigens / Q000276:immunology; D014162:Transfection
chemical_list: D018122:B7-1 Antigen; D018968:CD58 Antigens; D004768:Enterotoxins; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal