title: Aldosterone release from adrenal cells is inhibited by reduced oxygen levels in vitro during maturation in rabbits.
abstract: Hypoxia in vivo leads to a decrease in aldosterone not completely explained by extrinsic controllers of adrenal function including adrenocorticotrophic hormone, renin-angiotensin II, and K+. The dissociation of renin and aldosterone during acute hypoxia in vivo may be explained by the finding that aldosterone synthesis in adrenal cells is reversibly and specifically inhibited by decreases in O2 levels within the physiological range. The present study investigated whether the direct effect of acute decreases in O2 levels on aldosteronogenic pathway is altered during maturation. Adrenal cells (whole adrenals) were prepared from fetal (27 days gestation), neonatal (1 day), and infant (10 days) New Zealand White rabbits, and capsular cells were prepared from young (21 days) and adult (3 months) rabbits. All cells were dispersed with collagenase. Basal and cAMP-stimulated aldosterone production were assessed under two different levels of O2 (pO2 = 20.0 kPa or pO2 = 8.7 kPa). Decreased O2 levels significantly inhibited cAMP-stimulated aldosterone production in cells obtained from rabbits of all ages by 60 +/- 5% cAMP-stimulated aldosterone production was significantly lower in cells obtained from neonates and premature animals under both normoxic and reduced O2 conditions as compared with animals > or = 10 days old. Corticosterone production by cells obtained from adults and 21-day-old rabbits was unaffected by reduced O2 conditions suggesting a specific effect on the aldosterone pathway. The data demonstrate that the O2 sensitivity of the aldosterone pathway is present throughout development.
authors.affiliation: Program in Physical Therapy, Marquette University, Milwaukee, WI 53201, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Papanek
authors.forename: B M
authors.initials: BM
authors.lastname: Jankowski
authors.forename: H
authors.initials: H
authors.lastname: Raff
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000450:Aldosterone / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000032:analysis / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D000450:Aldosterone; D000242:Cyclic AMP; D010100:Oxygen; D003345:Corticosterone