title: Loss of bactericidal activity from contact lens storage solutions.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of lens storage on the bactericidal activities of disinfecting solutions.
  
  
  METHODS
  HYDROCURVEII (55% water content) soft contact lenses were soaked in OPTI-FREE Rinsing, Disinfecting, and Storage Solution (preserved with polyquaternium-1 [PQ-1]) or ReNu Multi-Purpose Solution (polyaminopropyl biguanide [PHMB]). After the lenses were removed, solutions were challenged with Pseudomonas aeruginosa and Staphylococcus aureus. Uptake of the antimicrobial agents by the lenses was evaluated by placing the lenses on plates seeded with S. aureus and observing for zones of growth inhibition.
  
  
  RESULTS
  The bactericidal activity of the PHMB preserved solution decreased after 4 hours of storage time and showed no activity after storage for 3 days. Lenses soaked in the PHMB preserved solution produced zones of inhibition. The results suggest that the PHMB in the solution accumulates in the lens. This decreases the levels of preservative in the solution that is no longer available during lens storage. Neither decrease in bactericidal activity nor accumulation of the PQ-1 preservative in the lens occurred during storage with the PQ-1 solution.
  
  
  CONCLUSIONS
  These results demonstrate that PQ-1 preserved solutions maintain bactericidal activity overnight and during prolonged storage without detectable uptake by lenses.
authors.affiliation: Alcon Laboratories, Inc., Fort Worth, TX 76134, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Rosenthal
authors.forename: M M
authors.initials: MM
authors.lastname: McDonald
authors.forename: R L
authors.initials: RL
authors.lastname: Schlitzer
authors.forename: R
authors.initials: R
authors.lastname: Abshire
authors.forename: R
authors.initials: R
authors.lastname: Stone
mesh_terms: D001645:Biguanides / Q000494:pharmacology*; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic; D006801:Humans; D011108:Polymers / Q000494:pharmacology*; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development*; D012044:Regression Analysis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D001645:Biguanides; D018639:Contact Lens Solutions; D011108:Polymers; D011310:Preservatives, Pharmaceutical; C064420:polyaminopropylbiguanide; C058655:polyquaternium 1