Dominant lethal mutations, heritable translocations, and unscheduled DNA synthesis induced in male mouse germ cells by glycidamide, a metabolite of acrylamide.
The hypothesis that acrylamide induces dominant lethal mutations and heritable translocations in male mice, not through direct adduction, but by conversion to the reactive epoxide, glycidamide, was investigated. Three studies, namely, induction of dominant lethal mutations, heritable translocations, and unscheduled DNA synthesis in spermatids, which were conducted earlier in this laboratory for acrylamide, were also performed for glycidamide to determine its mutagenic properties and to compare responses. Results of these studies are consistent with the proposal that in vivo conversion to glycidamide is responsible for the mutagenicity of acrylamide in male mice.
Generoso
W M
WM
Oak Ridge National Laboratory, Biology Division, TN37831, USA.
Sega
G A
GA
Lockhart
A M
AM
Hughes
L A
LA
Cain
K T
KT
Cacheiro
N L
NL
Shelby
M D
MD
D000818:Animals; D004260:DNA Repair; D004852:Epoxy Compounds / Q000633:toxicity*; D005260:Female; D005799:Genes, Dominant; D005804:Genes, Lethal; D005854:Germ Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D014178:Translocation, Genetic
D004852:Epoxy Compounds; C071834:glycidamide