title: Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing.
abstract: Rifampin susceptibility of 32 rifampin-resistant and 26 rifampin-susceptible Mycobacterium tuberculosis strains was analyzed by PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing within the 157-bp region of the rpoB gene (Ala500 to Val550). Two false-positive PCR-SSCP results were observed among the susceptible strains due to the silent mutation Gln513 (CAA-->CAG) and the deletion mutation Thr508 and Ser509. Another silent mutation [Leu511 (CTG-->CTA)], combined with the mutation Ser531-->Leu, was observed in a resistant strain. These results suggest that to rule out false-positive PCR-SSCP results, sequencing of the target DNA is required.
authors.affiliation: Department of Microbiology and Cancer Research Center, Seoul National University College of Medicine, Korea.
authors.forename: B J
authors.initials: BJ
authors.lastname: Kim
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: B H
authors.initials: BH
authors.lastname: Park
authors.forename: M A
authors.initials: MA
authors.lastname: Lyu
authors.forename: I K
authors.initials: IK
authors.lastname: Park
authors.forename: G H
authors.initials: GH
authors.lastname: Bai
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Cha
authors.forename: Y H
authors.initials: YH
authors.lastname: Kook
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005189:False Positive Reactions; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012293:Rifampin / Q000494:pharmacology*; D017384:Sequence Deletion
chemical_list: D012321:DNA-Directed RNA Polymerases; D012293:Rifampin