[Modification of Bacillus intermedius ribonuclease by dialdehyde dextran].
Conditions for modification of Bacillus intermedius ribonuclease with dialdehyde dextran was optimized by Box and Wilson's method to achieve the enzyme with higher activity. The generalized parameter including the yield of enzyme protein and enzyme stability during modification was used. The yield of activity and preparation characteristics depended on a number of enzyme-carrier bonds.
Kurinenko
B M
BM
Davydov
R E
RE
D001407:Bacillus / Q000201:enzymology*; D002352:Carrier Proteins / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry*; D004795:Enzyme Stability; D011485:Protein Binding; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism
D002352:Carrier Proteins; D003911:Dextrans; C026948:dextran dialdehyde; D012260:Ribonucleases