Biodegradation of hydrogen sulfide by a laboratory-scale immobilized Pseudomonas putida CH11 biofilter.
A heterotrophic Pseudomonas putida CH11 was isolated from livestock farming wastewater and applied for the treatment of H2S-containing gas. Extensive tests including removal characteristics, metabolic products, and removal efficiencies of H2S by P. putida CH11 were examined in batch and continuous systems. The optimum pH required to remove hydrogen sulfide was found in the range of 6-8. The maximum removal rate and the saturation constant were calculated to be Vm = 1.36 g S/day.kg dry bead and Ks = 45.9 ppm, respectively. The main metabolic product of H2S oxidation was determined to be elemental sulfur. When P. putida CH11 was immobilized within Ca alginate, the cells exhibited high H2S removal efficiency, in excess of 96%, at concentration of hydrogen sulfide from 10 to 150 ppm (flow rates of 36 and 72 L/h). These results suggest that P. putida CH11 immobilized within Ca alginate has the potential to be used as a H2S removal agent.
Chung
Y C
YC
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
Huang
C
C
Tseng
C P
CP
D000393:Air Pollutants; D000464:Alginates; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006862:Hydrogen Sulfide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007700:Kinetics; D008863:Microspheres; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D000393:Air Pollutants; D000464:Alginates; D006603:Hexuronic Acids; D007220:Industrial Waste; D020723:Glucuronic Acid; D006862:Hydrogen Sulfide