Tn5431 arose by transposition of Tn3 into Tn1721.
To further characterize Tn5431, which is composed of Tn3 and Tn1721, DNA sequencing was carried out. It was demonstrated that Tn5431 arose by transposition of Tn3 into Tn1721. Multiple mutations in the Tn3 tnpA gene have occurred, rendering the Tn3 portion of Tn5431 transposition defective and ensuring the simultaneous transposition of the antibiotic resistance determinants on Tn3 and Tn1721.
Zgur-Bertok
D
D
Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia. darja.zgur@uni-lj.si
Ambrozic
J
J
Grabnar
M
M
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012097:Repressor Proteins