Effects of interleukin-1 beta fragment (163-171) on progesterone and estradiol-17 beta release by bovine granulosa cells from different size follicles.
Recent studies indicate that interleukin-1 beta may play a role in ovarian function. To explore this possibility, we examined the effects of a fragment of interleukin-1 beta (163-171 aa) on progesterone and estradiol-17 beta production by bovine granulosa cells from small (< 5 mm) and large (> 8 mm) follicles; in addition we investigated the distribution of monocytes/macrophages, a possible source of interleukin-1 beta in the follicles, to determine a possible relationship between production of the cytokine and its activity. Bovine follicles were divided according to the size, granulosa cells were obtained by aspiration and plated at a density of 10(5) cells/well; then different concentrations of interleukin-1 beta fragment (0, 0.5, 5, 20 ng/ml) with or without bFSH were added for 24 h. Progesterone (P4) and estradiol-17 beta (E2) concentrations in media were evaluated by validated RIAs. Interleukin-1 beta (0.5 and 5 ng/ml) stimulated (P < 0.05) P4 output in both small and large follicles, while FSH-induced P4 output was reduced (P < 0.05) in large but not in small follicles by all the concentrations of peptide. FSH-induced E2 output was reduced (P < 0.05) in large, but not in small follicles. Immunohisto- and cytochemistry revealed a higher number of monocytes/macrophages in large follicles. It appears from our data that: (1) interleukin-1 beta fragment may modulate steroidogenesis in bovine granulosa cells depending on the stage of development of the follicle, and (2) the presence of monocytes/macrophages is related to this modulation.
Baratta
M
M
Instituto di Fisiologia Veterinaria, Università di Parma, Italy.
Basini
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G
Bussolati
S
S
Tamanini
C
C
D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*; D013194:Staining and Labeling
D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171); D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone