Effects of Schisandrin B and alpha-tocopherol on lipid peroxidation, in vitro and in vivo.
Effects of Schisandrin B (Sch B) and alpha-tocopherol (alpha-TOC) on ferric chloride (Fe3+) induced oxidation of erythrocyte membrane lipids in vitro and carbon tetrachloride (CCl4) induced lipid peroxidation in vivo were examined. While alpha-TOC could produce prooxidant and antioxidant effect on Fe(3+)-induced lipid peroxidation, Sch B only inhibited the peroxidation reaction. Pretreatment with alpha-TOC (3 mmol/kg/day x 3) did not protect against CCl4-induced lipid peroxidation and hepatocellular damage in mice, whereas Sch B pretreatment (0.3 mmol/3.0 mmol/kg/day x 3) produced a dose-dependent protective effect on the CCl4-induced hepatotoxicity. The ensemble of results suggests that the ability of Sch B to inhibit lipid peroxidation, while in the absence of pro-oxidant activity, may at least in part contribute to its hepatoprotective action.
Mak
D H
DH
Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay.
Ip
S P
SP
Li
P C
PC
Poon
M K
MK
Ko
K M
KM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D002712:Chlorides; D034242:Cyclooctanes; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D017705:Lignans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011083:Polycyclic Compounds / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002712:Chlorides; D034242:Cyclooctanes; D005290:Ferric Compounds; D017705:Lignans; D008563:Membrane Lipids; D011083:Polycyclic Compounds; C015499:schizandrin B; D014810:Vitamin E; D002251:Carbon Tetrachloride; C024555:ferric chloride