Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi.
The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.
Stevens
M K
MK
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USA.
Hassett
D J
DJ
Radolf
J D
JD
Hansen
E J
EJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D006191:Haemophilus ducreyi / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D013482:Superoxide Dismutase