title: Vanadyl sulphate differently influences insulin response to glucose in isolated pancreas of normal rats after in vivo or in vitro exposure.
abstract: The effect of the antidiabetic agent vanadyl sulphate (VOSO4) on the endocrine pancreas function of normal rats was studied using the isolated pancreas preparation. A short-term (8 days) i.p. treatment (15 mg/kg per day) resulted in attenuation of high glucose-stimulated insulin release, at day 9 but also at days 19, i.e., after full recovery of appetite and weight, while blood and pancreas vanadium concentrations were still elevated. Six months of oral VOSO4 treatment (0.75 mg/ml in drinking water) resulted in elevated vanadium concentrations while glucose-stimulated insulin release was attenuated as compared to pair-fed animals. Conversely, when directly perfused in pancreas, VOSO4 potentiated glucose-stimulated insulin release. These apparently opposite effects may be related to the ability of VOSO4 to exert both peripheral insulinomimetic effects-leading to chronic reduction in insulin demand-, and a direct pancreatic insulinotropic activity.
authors.affiliation: Laboratoire de Pharmacologie (EA 2035), Faculté de Pharmacie, Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: Cadène
authors.forename: R
authors.initials: R
authors.lastname: Gross
authors.forename: P
authors.initials: P
authors.lastname: Poucheret
authors.forename: J J
authors.initials: JJ
authors.lastname: Mongold
authors.forename: P
authors.initials: P
authors.lastname: Masiello
authors.forename: M
authors.initials: M
authors.lastname: Roye
authors.forename: G
authors.initials: G
authors.lastname: Ribes
authors.forename: J J
authors.initials: JJ
authors.lastname: Serrano
authors.forename: G
authors.initials: G
authors.lastname: Cros
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017968:Vanadium Compounds / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D017968:Vanadium Compounds; C034028:vanadyl sulfate; D005947:Glucose