title: Comparative analysis of HSV-1 temperature mutants proteins and their reactivity.
abstract: Protein's characteristic of temperature sensitive (ts 28 degrees C) and temperature resistant (tr 39 degrees C) mutants and native HSV-1 strain was performed. Electrophoretic and Western-blott analysis of virus proteins and their reactivity tested by immunoenzymatic staining was the main subject of the presented paper. There were found no significant differences in the protein structure between native strain and its temperature clones in electrophoresis. It is important that slight differences observed in protein's antigenic reactivity of ts 28 degrees C (54 kDa) and tr 39 degrees C (99-100; 160 kDa), do not influence on the possible application of the mutants for immunization and challenging.
authors.affiliation: National Institute of Hygiene, Department of Virology, Warszawa.
authors.forename: B
authors.initials: B
authors.lastname: Litwińska
authors.forename: A
authors.initials: A
authors.lastname: Biesiadecka
authors.forename: W
authors.initials: W
authors.lastname: Gut
authors.forename: M
authors.initials: M
authors.lastname: Kańtoch
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D018259:Herpesvirus 1, Human / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008970:Molecular Weight; D009154:Mutation; D013696:Temperature; D015678:Viral Structural Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D015678:Viral Structural Proteins