Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during Drosophila development.
Most cell types in multicellular eukaryotes exit from the mitotic cell cycle before terminal differentiation. We show that the dacapo gene is required to arrest the epidermal cell proliferation at the correct developmental stage during Drosophila embryogenesis. dacapo encodes an inhibitor of cyclin E/cdk2 complexes with similarity to the vertebrate Cip/Kip inhibitors. dacapo is transiently expressed beginning late in the G2 phase preceding the terminal division (mitosis 16). Mutants unable to express the inhibitor fail to arrest cell proliferation after mitosis 16 and progress through an extra division cycle. Conversely, premature dacapo expression in transgenic embryos results in a precocious G1 arrest.
Lane
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ME
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen,Federal Republic of Germany.
Sauer
K
K
Wallace
K
K
Jan
Y N
YN
Lehner
C F
CF
Vaessin
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D042846:CDC2-CDC28 Kinases; D002455:Cell Division; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D016213:Cyclins / Q000037:antagonists & inhibitors*; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D004791:Enzyme Inhibitors; D000078404:Epidermal Cells; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D006131:Growth Inhibitors / Q000235:genetics*; D019476:Insect Proteins / Q000502:physiology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment
D016213:Cyclins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D019476:Insect Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D012333:RNA, Messenger; C103336:dap protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; C495887:cdk2 protein, Drosophila