Prominence of apolipoproteins B, (a), and E in the intimae of coronary arteries in transplanted human hearts: geographic relationship to vessel wall proteoglycans.
BACKGROUND
Transplant arteriopathy (TA) is characterized by vessel wall thickening with prominent glycosaminoglycan and lipid deposits. In this light, we have recently demonstrated the distribution of proteoglycans in allograft coronary arteries. The aim of this study is to examine the distribution of apolipoproteins within allograft coronary arteries and to investigate their localization in relation to proteoglycans.


METHODS
Particular transverse sections of left and right epicardial coronary arteries from 46 human cardiac allografts, 11 normal hearts, and 11 hearts with native atherosclerosis were stained immunohistochemically for apolipoprotein B, apolipoprotein (a) (apo[a]), apolipoprotein E (apoE), biglycan, decorin, and versican by use of an automated immunostainer.


RESULTS
Apo(a) and apoE immunopositivity in TA was much more intense than that in native atherosclerosis, whereas the reverse was true for apoB. Prominent apoE deposits were evident circumferentially in endothelia and extracellularly in superficial intima of mildly diseased TA, as well as in deeper intima of severely diseased TA. Apo(a) had a staining pattern very similar to apoE except for a patchy deposition also seen in TA media. The intimal areas staining prominently with apoE or apo(a) in TA arteries corresponded very closely to the areas with proteoglycan deposits, especially versican.


CONCLUSIONS
The distinctive patterns of apolipoprotein accumulation in TA and native atherosclefosis appear to reflect different pathogenetic processes in the two conditions. The colocalization of proteoglycans and apolipoproteins in TA intima supports the hypothesis that interactions between proteoglycans and apolipoproteins influence lipid retention and overload in allograft coronary arteries.
Lin
H
H
Department of Pathology, University of British Columbia, Vancouver, Canada.
Ignatescu
M
M
Wilson
J E
JE
Roberts
C R
CR
Horley
K J
KJ
Winters
G L
GL
Costanzo
M R
MR
McManus
B M
BM
D001053:Apolipoproteins / Q000032:analysis*; D001055:Apolipoproteins B / Q000032:analysis*; D001057:Apolipoproteins E / Q000032:analysis*; D053241:Apoprotein(a); D003324:Coronary Artery Disease / Q000378:metabolism; D003331:Coronary Vessels / Q000737:chemistry*; D005260:Female; D016027:Heart Transplantation; D006801:Humans; D017270:Lipoprotein(a); D008297:Male; D011509:Proteoglycans / Q000032:analysis*; D017539:Tunica Intima / Q000737:chemistry
D001053:Apolipoproteins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D017270:Lipoprotein(a); D011509:Proteoglycans; D053241:Apoprotein(a)