Patterns of cellular activation after tendon injury.
Mechanisms which lead to disabling adhesions following flexor tendon surgery of the hand were investigated in a rabbit model which was used to assess the relative response of the cells of the synovial sheath, epitenon and the endotenon to injury. A transverse laceration, cutting through 50% of the tendon, was made just outside the synovial sheath on the flexor aspect of the flexor digitorum profundus tendon. The synovial sheath was preserved intact. Using monoclonal antibodies for localizing specific inflammatory markers, we were able to follow the response and activity of the synovial sheath, epitenon and endotenon with respect to these markers at various times after surgery. Our findings suggest that the synovial sheath and the epitenon are relatively more reactive in the early period after injury, as judged by a range of inflammatory indices with the notable exception of the expression of the potent neovascularizing agent, basic fibroblast growth factor (bFGF).
Khan
U
U
Department of Plastic and Reconstructive Surgery, University College, London, UK.
Edwards
J C
JC
McGrouther
D A
DA
D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000502:physiology; D006133:Growth Substances / Q000502:physiology; D018836:Inflammation Mediators / Q000032:analysis*; D008262:Macrophage Activation / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen / Q000502:physiology; D011817:Rabbits; D013708:Tendon Injuries / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D000267:Tissue Adhesions; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology; D014945:Wound Healing / Q000276:immunology*
D006133:Growth Substances; D018836:Inflammation Mediators; D018809:Proliferating Cell Nuclear Antigen; D019010:Vascular Cell Adhesion Molecule-1; D016222:Fibroblast Growth Factor 2