title: Protective mechanism of high-density lipoprotein against endotoxemia in chronic alcohol ingestion.
abstract: In the present study, we evaluated the role of high-density lipoprotein (HDL) as an endotoxin-binding protein in chronically alcohol-fed rats. Although the blood endotoxin level was significantly elevated in chronic ethanol-loaded rats, compared with control rats, serum tumor necrosis factor (TNF), ALT, and lactate dehydrogenase were not elevated. Serum HDL and its endotoxin-binding capacity were significantly increased in chronic ethanol-loaded rats. When Kupffer cells isolated from control and chronic ethanol-loaded rats were cultured in the medium containing 3 to 30 mg/dl HDL and endotoxin (500 ng/ml), endotoxin uptake and TNF production of Kupffer cells were decreased in proportion to the concentration of HDL in the medium. These results suggest that the increase in endotoxin-binding capacity of HDL may serve as a protective mechanism against endotoxin in chronic ethanol-loaded rats.
authors.affiliation: 3rd Department of Internal Medicine, Nara Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kitano
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: E
authors.initials: E
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Kikukawa
authors.forename: M
authors.initials: M
authors.lastname: Morimura
authors.forename: S
authors.initials: S
authors.lastname: Tsujita
authors.forename: I
authors.initials: I
authors.lastname: Nagamoto
authors.forename: K
authors.initials: K
authors.lastname: Hoppo
authors.forename: Y
authors.initials: Y
authors.lastname: Nakatani
authors.forename: T
authors.initials: T
authors.lastname: Nakatani
authors.forename: T
authors.initials: T
authors.lastname: Tsujii
mesh_terms: D000437:Alcoholism / Q000276:immunology*; D000818:Animals; D019446:Endotoxemia / Q000276:immunology*; D007728:Kupffer Cells / Q000276:immunology; D008075:Lipoproteins, HDL / Q000502:physiology*; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology*; D008111:Liver Function Tests; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D008075:Lipoproteins, HDL; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; C025357:endotoxin binding proteins