[Comparative study of the effect of fatty acids and certain spin effectors on quaternary structure and spin state of methemoglobin].
Cysteine beta-93 SH-groups of horse and bovine methemoglobins (metHb) were modified by N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide. Effects of pH, F-, CN-, oleic acid, and sodium dodecyl sulfate on spin and conformational dynamics of the protein were studied by electron and EPR spectroscopy. Oleic acid and CN- had similar effects on both parameters. Relationship between spin and conformational transformations of metHb was elucidated. Effect of oleic acid can be due to increased conformational mobility of C-terminus of metHb beta-chains.
Bovdeĭ
N A
NA
Kiseleva
S N
SN
Kiselev
P A
PA
D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000494:pharmacology*; D006736:Horses; D008706:Methemoglobin / Q000737:chemistry*; D011487:Protein Conformation; D013113:Spin Labels
D005227:Fatty Acids; D013113:Spin Labels; D008706:Methemoglobin