title: The structure of stewartan, a capsular polysaccharide produced by Erwinia stewartii strain DC283.
abstract: Stewartan is a capsular polysaccharide produced by Erwinia stewartii, the causative agent of Stewart's wilt of maize. The structure of stewartan is shown, by a combination of methylation analysis, Li/ethylenediamine degradation, 1D and 2D NMR spectroscopy, partial acid hydrolysis and isolation of oligosaccharides, Smith degradation, MALDI-TOFMS analysis and exoglycosidase digestion, to have the following repeating unit: [sequence: see text]
authors.affiliation: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA.
authors.forename: B Y
authors.initials: BY
authors.lastname: Yang
authors.forename: J S
authors.initials: JS
authors.lastname: Gray
authors.forename: R
authors.initials: R
authors.lastname: Montgomery
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004885:Erwinia / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006026:Glycoside Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010935:Plant Diseases / Q000209:etiology; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D017421:Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D003313:Zea mays / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C100770:stewartan; D006026:Glycoside Hydrolases