A transcript exhibiting homology to endogenous rat retroviral-like elements is regulated by p53.
Rat embryo fibroblasts transformed with HPV-16 E7 and the Ha-ras oncogene (ER clones) fall into two distinct groups based on their endogenous p53 status, wild-type or mutant. We have taken advantage of such clones in order to study the p53 target genes by the differential display method of RNA fingerprinting. We have identified a cDNA clone, clone 16, that recognises a large transcript on Northern blots. The clone 16 transcript is overexpressed in ER cell lines that express wild-type p53 compared with ER cell lines that express mutant p53. Similar to the waf1/p21 gene, which is transcriptionally activated in cells treated with ionizing radiation in a p53-dependent manner, the clone 16 transcript was also induced in response to cell irradiation. The sequence of clone 16 exhibits a high homology to two members of RAL retroviral-like elements.
Gangopadhyay
S B
SB
The Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Ontario, Canada.
Peacock
J W
JW
Benchimol
S
S
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts; D016158:Genes, p53 / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D027383:Papillomaviridae; D012313:RNA / Q000378:metabolism; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025721:WT1 Proteins
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025721:WT1 Proteins; D012313:RNA; D004247:DNA