Adhesiveness for extracellular matrices and lysosomal enzyme release from normal and beta 2 integrin-deficient bovine neutrophils.
The adhesiveness of control and CD18-deficient bovine neutrophils on culture plates precoated with collagen I, collagen IV, fibronectin and laminin was measured to evaluate the possible factors for adherence to extracellular matrices. The release of N-acetyl-beta-D-glucosaminidase (NAGase) from control and CD18-deficient neutrophils stimulated with complement receptor type 3 (CR3) or Fc receptor dependent stimuli was also evaluated. The adhesive activities of CD18-deficient neutrophils to collagen I, collagen IV and fibronectin were significantly diminished (P < 0.05); however, similar adhesion to laminin was observed in CD18-deficient neutrophils and control neutrophils. The adhesive activity of control neutrophils on uncoated plates increased 2.5 times (P < 0.05) with the presence of PMA. The mean activities for NAGase release from CD18-deficient neutrophils stimulated with opsonized zymosan and aggregated bovine immunoglobulin G (Agg-IgG) were 46.7 and 82.7% that of the control neutrophils, respectively. The Agg-IgG-induced NAGase release from control and CD18-deficient neutrophils was eliminated by H7, a protein kinase C inhibitor. These results support that an association between CR3 and Fc receptors on neutrophils appears to play an essential role in neutrophil functions.
Nagahata
H
H
Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Higuchi
H
H
Noda
H
H
Tamoto
K
K
Kuwabara
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D018821:CD18 Antigens / Q000502:physiology*; D002417:Cattle; D002448:Cell Adhesion; D003094:Collagen; D005109:Extracellular Matrix; D005260:Female; D005353:Fibronectins; D007797:Laminin; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000276:immunology*; D008247:Lysosomes / Q000201:enzymology; D016177:Macrophage-1 Antigen / Q000502:physiology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D009895:Opsonin Proteins; D011961:Receptors, Fc / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D018821:CD18 Antigens; D005353:Fibronectins; D007797:Laminin; D016177:Macrophage-1 Antigen; D009895:Opsonin Proteins; D011961:Receptors, Fc; D003094:Collagen; D015054:Zymosan; D000118:Acetylglucosaminidase; D013755:Tetradecanoylphorbol Acetate