Enhanced delivery of [125I]glial cell line-derived neurotrophic factor to the rat CNS following osmotic blood-brain barrier modification.
Subsequent to osmotic (mannitol-induced) blood-brain barrier (BBB) opening, [125I]glial cell line-derived neurotrophic factor (GDNF) was detected throughout the ventricular system, associated with the ependymal cell layer and extracellular matrix and to some extent penetrated into the cerebral cortex, subcortical gray matter, substantia nigra, septum, eye and optic nerve at 1 and 24 h following an intracarotid administration. Our study indicates that osmotic opening of the BBB allows for extensive distribution of GDNF throughout the central nervous system (CNS).
Jiao
S
S
AMGEN Inc., Department of Neuroscience, Thousand Oaks, CA 91320-1789, USA.
Miller
P J
PJ
Lapchak
P A
PA
D000818:Animals; D001345:Autoradiography; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D004234:Diuretics, Osmotic / Q000494:pharmacology; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007457:Iodine Radioisotopes; D008353:Mannitol / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009995:Osmosis; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism
D004234:Diuretics, Osmotic; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007457:Iodine Radioisotopes; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D008353:Mannitol