The structure of the exopolysaccharide produced by the halophilic Archaeon Haloferax mediterranei strain R4 (ATCC 33500).
The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as [formula: see text]
Parolis
H
H
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
L A
LA
Boán
I F
IF
Rodríguez-Valera
F
F
Widmalm
G
G
Manca
M C
MC
Jansson
P E
PE
Sutherland
I W
IW
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000032:analysis; D001105:Archaea / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D010504:Periodic Acid / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D017421:Sequence Analysis; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013463:Sulfuric Acid Esters / Q000737:chemistry*
D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D013463:Sulfuric Acid Esters; D010504:Periodic Acid; C009288:metaperiodate; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine