Detection of Chlamydia trachomatis by immunofluorescence, papanicolaou and immunoperoxidase in women with leucorrhea.
Bacteriological, Papanicolaou, direct immunofluorescense (DIF) and immunoperoxidase studies were done in 245 samples from women with vaginal discharge and the frequency of Chlamydia trachomatis was investigated. The samples were obtained from two hospitals of the Public Health Services of Mexico City. Samples were taken from the endocervical and posterior fornix areas and streaked in different cultures media for the isolation and microbiological differentiation. Smears were done and stained by Gram, Papanicolaou, DIF and immunoperoxidase techniques and the presence of cytological alterations and Chlamydia trachomatis was investigated. The microorganism was detected in 8 patients by the three methods, only 4 of these were found by DIF, 7 by cytological and 6 by immunoperoxidase techniques. The infection caused by C. trachomatis cytologically was associated with mild or moderate dysplasia but not with some special microorganism.
Reyes-Maldonado
E
E
Departamento de Morfología, Escuela Nacionalde Ciencias Biológicas, I.P.N. Prol. Carpio y Plan de Ayals, México, D.F., México.
Díaz-Fuente
L A
LA
Gonzàlez-Bonilla
C V
CV
Esquer-Mendivi
M
M
Jiménez
E Z
EZ
Deleón
R I
RI
D000293:Adolescent; D000328:Adult; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D002175:Candida / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D015994:Incidence; D007973:Leukorrhea / Q000209:etiology* / Q000382:microbiology / Q000469:parasitology; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D065006:Papanicolaou Test; D014246:Trichomonas vaginalis / Q000302:isolation & purification; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000453:epidemiology; D002575:Uterine Cervicitis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology