title: cGMP-mediated effects on the physiology of bovine and human retinal Müller (glial) cells.
abstract: 1. Whole-cell currents of freshly dissociated or cultured Müller cells from human and bovine retinas were studied using the perforated-patch and standard whole-cell recording techniques. 2. We found that internal perfusion of cGMP or external exposure to 8-bromo-cGMP activated a calcium permeable, non-selective cation current in Müller cells, the principal glial cells of the retina. In addition, the activity of calcium-activated potassium channels increased markedly. These currents were minimally affected by cAMP. 3. Molecular studies using the reverse transcription-polymerase chain reaction demonstrated that human müller cells in culture contain transcripts closely related to the rod cyclic nucleotide-gated (CNG) channel. 4. Since guanylate cyclase is a known target for nitric oxide (NO), we tested the effect of NO donors on Müller cell currents. These agents induced currents that were qualitatively similar to those activated by cGMP. 5. Our experiments support the idea that the NO-cGMP pathway regulates the physiology of Müller cells and may play a role in integrating neuron-glia interactions in the retina.
authors.affiliation: Department of Ophthalmology, University of Michigan, Ann Arbor 48105, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kusaka
authors.forename: I
authors.initials: I
authors.lastname: Dabin
authors.forename: C J
authors.initials: CJ
authors.lastname: Barnstable
authors.forename: D G
authors.initials: DG
authors.lastname: Puro
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002417:Cattle; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D008958:Models, Molecular; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000502:physiology*
chemical_list: D007473:Ion Channels; D002104:Cadmium; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D006152:Cyclic GMP