Estrogen regulation of preproenkephalin messenger RNA in the forebrain of female mice.
We studied the effects of 10 micrograms of 17-beta-estradiol-3-benzoate treatment on preproenkephalin (PPE) mRNA expression in female ovariectomized (OVX) Swiss Webster mice after 0, 1, 6, 12, 24, or 48 h, using the in situ hybridization technique. The VMH showed a 1.6- and 3.3-fold increase in PPE mRNA levels after 24 and 48 h of estrogen treatment (respectively) when compared to OVX females. No differences at 1, 6 or 12 h of estrogen treatment groups were observed compared to control groups. PPE mRNA levels were also increased at 24 and 48 h after estrogen treatment in the posterior medial nucleus of the amygdala (MeAmyg) by 3.3- and 2.5-fold, respectively, and in the arcuate nucleus (ARC) by 2- and 1.9-fold, respectively. No effects of estrogen were observed on PPE mRNA levels in the caudate-putamen (CPu) or the posterior lateral cortical nucleus of the amygdala (plCoAmyg). Furthermore, basal levels of PPE mRNA expression in the VMH and MeAmyg of female mice were lower than those observed in rats, although levels in the CPu, plCoAmyg, and ARC were similar between females of the two species. In conclusion, we have found two differences between the species. First, Swiss mice demonstrated a slower time course of estrogen induction of PPE mRNA in the VMH, ARC. and MeAmyg compared to female rats. Second, there are differences in basal levels of PPE in the MeAmyg and VMH.
Quiñones-Jenab
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Laboratory of Neurobiology and Behavior, Rockefeller University, New York, NY 10021, USA.
Ogawa
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Jenab
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Pfaff
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DW
D000818:Animals; D004745:Enkephalins / Q000235:genetics*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D017403:In Situ Hybridization; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D016548:Prosencephalon / Q000187:drug effects* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D004958:Estradiol; C033660:preproenkephalin