title: Cytokine modulation of inhibin secretion in cultured rat granulosa cells.
abstract: In the present study, we examined the effects of tumour necrosis factor alpha (TNF alpha) and interleukin-1 beta (IL-1 beta) on inhibin secretion by cultured rat granulosa cells using immunoblotting and two-site enzyme immunoassay for inhibin A (alpha-beta A dimer). FSH stimulated the secretion of the inhibin alpha-beta A dimer (32 kDa) by the cells in a dose-dependent manner. In addition to the predominant 32 kDa inhibin alpha-beta A dimer staining, staining of minor immunoreactive bands was also enhanced by FSH. TNF alpha alone did not have any effect on inhibin secretion. Immunoblot analyses using an antiserum against alpha-subunit and an antiserum against beta A-subunit revealed a dose-dependent inhibition by TNF alpha of FSH-stimulated secretion of inhibin by rat granulosa cells. Similarly, TNF alpha inhibited in a dose-dependent manner FSH-stimulated inhibin secretion when measured using a two-site enzyme immunoassay. IL-1 beta alone did not exert any effect on inhibin secretion but it inhibited FSH-stimulated inhibin release in a dose-dependent manner (using both immunoblotting and a two-site assay for inhibin A). The present observations suggest that TNF alpha and IL-1 beta inhibit gonadotrophin-stimulated inhibin production by cultured rat granulosa cells.
authors.affiliation: Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Yamoto
authors.forename: H
authors.initials: H
authors.lastname: Otani
authors.forename: R
authors.initials: R
authors.lastname: Nakano
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007265:Inhibins / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D015846:Monokines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D015846:Monokines; D014409:Tumor Necrosis Factor-alpha; D007265:Inhibins