Evaluation of urinary pyridinoline in healthy adults and patients with rheumatoid arthritis by an improved high-performance liquid chromatographic assay.
A modified method for the determination of pyridinoline using high-performance liquid chromatography (HPLC) has been applied to analyze the urine of healthy adults and patients with rheumatoid arthritis. The concentrations of pyridinoline in the urine of healthy Japanese men and women (average age: 50.6 and 51.8 yr, respectively) during morning fasting were 28.8 +/- 2.8 (SEM) and 34.8 +/- 3.5 nmol/mmol creatinine, respectively. In the case of patients with rheumatoid arthritis, the urinary concentration of pyridinoline was 49.1 +/- 8.3 nmol/mmol creatinine (women, average age, 61.1 yr), which was significantly higher than that of healthy women of the same age (p < 0.05). This study supports the application of HPLC for urinary pyridinoline analysis and as a screening test for patients with bone disease.
Otsuka
M
M
Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan.
Kurosaka
R
R
Kim
J S
JS
Kawai
S
S
Arakawa
N
N
D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000652:urine*; D001172:Arthritis, Rheumatoid / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D000596:Amino Acids; C015484:pyridinoline; D003404:Creatinine