Salt-Sensitive and Auxotrophic Mutants of Halomonas elongata and H. meridiana by Use of Hydroxylamine Mutagenesis
The killing action and induced mutagenicity inhydroxylamine (HA)-treated cells of two moderately halophilic species of thegenus Halomonas, H. elongata and H. meridiana, wereinvestigated. A high sensitivity of H. elongata and especially ofH. meridiana to HA was found. The efficiency of the mutagenicityobtained with the HA treatment was tested at different salinities. Optimalexperimental conditions for HA mutagenesis of these two moderate halophileswere determined. A clear, efficient mutagenicity of both H. elongataand H. meridiana after HA mutagenesis was achieved. The optimalprocedures yielded a number of useful auxotrophic mutants of H.elongata as well as different salt-sensitive mutants of H.elongata and H. meridiana for further studies. Some of theselatter mutants appeared to be affected in the synthesis of compatiblesolutes.
C&aacute;novas
D
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
Vargas
C
Ventosa
A
Nieto
JJ