Synthesis of alanine and leucine by reductive amination of 2-oxoic acid with combination of hydrogenase and dehydrogenase.
Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leucine synthesis was also carried out by the combination of NADH regeneration system and leucine dehydrogenase (LeuDH). The conversion of 4-methyl-2-oxovalerate to leucine was 60% after 1.5 h.
Hasumi
F
F
Department of Chemistry and Biochemistry, Numazu College of Technology, Shizuoka, Japan.
Fukuoka
K
K
Adachi
S
S
Miyamoto
Y
Y
Okura
I
I
D000409:Alanine / Q000138:chemical synthesis*; D006864:Hydrogenase; D007930:Leucine / Q000138:chemical synthesis*; D009243:NAD; D010088:Oxidoreductases
D009243:NAD; D010088:Oxidoreductases; D006864:Hydrogenase; D007930:Leucine; D000409:Alanine