Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia.
To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.
Porter
K R
KR
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland, USA.
Mascola
J R
JR
Hupudio
H
H
Ewing
D
D
VanCott
T C
TC
Anthony
R L
RL
Corwin
A L
AL
Widodo
S
S
Ertono
S
S
McCutchan
F E
FE
Burke
D S
DS
Hayes
C G
CG
Wignall
F S
FS
Graham
R R
RR
D000595:Amino Acid Sequence; D001665:Binding Sites; D015704:CD4 Antigens / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D005260:Female; D005838:Genotype; D015488:HIV Antigens / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006679:HIV Seropositivity / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007214:Indonesia / Q000453:epidemiology; D008297:Male; D008889:Military Personnel; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D012703:Serotyping
D015704:CD4 Antigens; D004279:DNA, Viral; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D007106:Immune Sera; D010446:Peptide Fragments