title: Activation of endogenous c-Src or a related tyrosine kinase by intracellular (pY)EEI peptide increases voltage-operated calcium channel currents in rabbit ear artery cells.
abstract: The effect of activation of endogenous c-Src tyrosine kinase by (pY)EEI peptide was examined on voltage-operated calcium channel (VOC) currents in arterial smooth muscle cells. In single rabbit ear artery cells intracellular application of (pY)EEI peptide increased calcium channel currents. Inactive, non-phosphorylated YEEI peptide had no effect on currents. Peptide-A, a 21 amino acid inhibitor of c-Src inhibited currents and prevented the effect of (pY)EEI peptide on calcium channel currents. These results indicate that activation of intrinsic c-Src increases VOC and support a role for c-Src in the regulation of VOC in vascular smooth muscle cells.
authors.affiliation: Department of Clinical Pharmacology, Imperial College School of Medicine at St. Mary's, London, UK. v.wijetunge@ic.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Wijetunge
authors.forename: A D
authors.initials: AD
authors.lastname: Hughes
mesh_terms: D000818:Animals; D001158:Arteries / Q000201:enzymology / Q000378:metabolism*; D000081247:CSK Tyrosine-Protein Kinase; D015220:Calcium Channels / Q000187:drug effects*; D004423:Ear / Q000098:blood supply; D004789:Enzyme Activation; D008564:Membrane Potentials / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011817:Rabbits; D019061:src-Family Kinases
chemical_list: D015220:Calcium Channels; D010455:Peptides; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases