title: Cytochemical and immunological phenotying of acute nonlymphoblastic leukemia in Thai patients.
abstract: Using morphology and cytochemical reaction, we could subclassify-according to FAB classification, 51 of our 56 cases of acute nonlymphoblastic leukemia (ANLL) as M1-M5. Five cases were undifferentiated. Using the immunophenotypic method, we could subclassify 51 of these patients as M1-M4. In addition, 3 cases of undifferentiated leukemia by the prior method were each classified as M1, M3, and myelo-megakaryoblastic leukemia. Correlation of ANLL subtype classification according to each method was not good. However, combination of both methods, using immunophenotypic analysis as a supplement would better subclassify the disease. One of the remaining 2 cases of undifferentiated leukemia was also shown to be myelo-megakaryoblastic leukemia by a positive platelet peroxidase reaction by ultrastructural cytochemistry. Thus, combination of these 3 methods could diagnose and subclassify 55 of the 56 cases (98%) of our ANLL patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Pokatikorn
authors.forename: S
authors.initials: S
authors.lastname: Apibal
authors.forename: V
authors.initials: V
authors.lastname: Atichartakarn
authors.forename: S
authors.initials: S
authors.lastname: Sriurairatana
authors.forename: D
authors.initials: D
authors.lastname: Chiewsilp
authors.forename: P
authors.initials: P
authors.lastname: Thitikawin
authors.forename: C
authors.initials: C
authors.lastname: Timvipark
mesh_terms: D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016130:Immunophenotyping / Q000379:methods*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D013785:Thailand