title: A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection.
abstract: We devised a novel Western blot (WB) test for anti-human cytomegalovirus (HCMV) immunoglobulin M (IgM) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the most immunogenic nonstructural protein p52 (ppUL44). This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV infection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-specific IgM.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Bologna, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Lazzarotto
authors.forename: G T
authors.initials: GT
authors.lastname: Maine
authors.forename: P
authors.initials: P
authors.lastname: Dal Monte
authors.forename: A
authors.initials: A
authors.lastname: Ripalti
authors.forename: M P
authors.initials: MP
authors.lastname: Landini
mesh_terms: D000328:Adult; D000465:Algorithms; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western / Q000379:methods*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004268:DNA-Binding Proteins / Q000276:immunology; D005069:Evaluation Studies as Topic; D005260:Female; D016027:Heart Transplantation; D006801:Humans; D016867:Immunocompromised Host; D007075:Immunoglobulin M / Q000097:blood*; D016030:Kidney Transplantation; D010750:Phosphoproteins; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004268:DNA-Binding Proteins; C072217:ICP36 protein, Cytomegalovirus; D007075:Immunoglobulin M; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C056367:pp150 protein, Cytomegalovirus