In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis.
We developed an efficient differentiation induction system (OP9 system) from mouse embryonic stem (ES) cell to blood cells by coculture on a novel stromal cell line, OP9. This stromal cell line does not produce functional macrophage colony-stimulating factor (M-CSF) and presumably the deficiency of M-CSF production of OP9 cells might induce preferential differentiation of ES cells into hematopoietic cells other than the monocyte-macrophage lineage. ES cells gave rise to embryonic primitive erythrocytes, and then adult type definitive erythrocytes, myeloid and B lineage cells. The OP9 system should facilitate to elucidate the molecular mechanisms of hematopoietic system development and hematopoietic cell differentiation.
Nakano
T
T
Department of Molecular Cell Biology, Osaka University, Japan. tnakano@biken.osakau.ac.jp
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D004920:Erythropoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D051379:Mice