Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype.
Members of the dynamin superfamily are GTPases which have been shown to support receptor-mediated endocytosis in vivo and bind to growth factor receptor-associated proteins in vitro. In acinar cells of the pancreas, receptor-mediated endocytosis is very important for the recycling of membranes after secretory granule release. Therefore, characterization of the molecular machinery responsible for this process is critical for a better understanding of this phenomenon. In this study we sought to determine the expression pattern of the endocytic GTPase dynamin II during pancreatic acinar cell differentiation in developing rat embryos and in dexamethasone-treated AR42J cells using Western blot, Northern blot, and immunocytochemical analyses. During pancreatic development, dynamin immunoreactivity is almost undetectable until day E17 but undergoes significant upregulation in acinar cells starting at E18. In addition, the levels of dynamin mRNA and protein in AR42J cells increase approximately threefold during dexamethasone-induced acinar differentiation. The increase in dynamin levels that occurs in both embryonic pancreatic cells and dexamethasone-treated AR42J cells correlates with the establishment of a more differentiated acinar phenotype. Therefore, these results suggest a potential role for dynamin in supporting receptor-mediated endocytosis in mature pancreatic acinar cells.
Cook
T A
TA
Saint Marys Hospital, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA.
Mesa
K J
KJ
Gebelein
B A
BA
Urrutia
R A
RA
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000276:immunology*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015854:Up-Regulation
D012333:RNA, Messenger; D003907:Dexamethasone; D020558:GTP Phosphohydrolases; D034281:Dynamins