An antigenic water-soluble glucogalactomannan extracted from cell walls of Paecilomyces fumosoroseus and Paecilomyces farinosus.
The water-soluble fraction (F1S) obtained after solubilizing in alkali the cell walls of four strains of Paecilomyces fumosoroseus and two of Paecilomyces farinosus amounted to 8.3-14.5% of the dry cell wall material. Two polysaccharides, F1S-A (13-20%) and F1S-B (57-68%) were separated from F1S by gel permeation through Sepharose CL-6B. 1H and 13C NMR spectra of F1S-B were recorded and showed analogous structural features in the six isolates of the two species. The fractions isolated from P. fumosoroseus strain CBS 375.70 were subjected to structural analysis and shown to be a (1-->4)-alpha-glucan (F1S-A) and a branched (1-->6)-mannan with terminal residues of beta-galactopyranose (F1S-B). Polyclonal antibodies against the latter polysaccharide were obtained (titre 1/8000). These antibodies reacted specifically with the F1S-B polysaccharides obtained from the four strains of P. fumosoroseus and the two strains of P. farinosus, but they did not react with similar fractions from other species of the same or related genera. The antibodies specifically stained P. fumosoroseus hyphae in indirect immunofluorescence tests.
Domenech
J
J
Centro de Investigaciones Biológicas, Madrid, Spain.
Barasoain
I
I
Prieto
A
A
Gómez-Miranda
B
B
Bernabé
M
M
Leal
J A
JA
D000946:Antigens, Fungal / Q000737:chemistry / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002247:Carbon Isotopes; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification*; D010143:Paecilomyces / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D013045:Species Specificity
D000946:Antigens, Fungal; D002247:Carbon Isotopes; D008351:Mannans; D011134:Polysaccharides; D006859:Hydrogen