title: Effect of transforming growth factor beta-1 in endotoxin-induced uveitis.
abstract: OBJECTIVE
  Transforming growth factor beta-1 (TGF beta-1) can modulate inflammation. Endotoxin-induced uveitis (EIU) is characterized by acute ocular inflammation related to the release of cytokines, including interleukin (IL)-6. The authors investigated the effect of TGF beta-1 on EIU in mice.
  
  
  METHODS
  Three independent experiments were performed. Endotoxin-induced uveitis was induced in C3H/HeN mice by an injection of 200 micrograms of lipopolysaccharide (LPS). Two micrograms of TGF beta-1 in 0.1 ml phosphate-buffered saline (PBS) or 0.1 ml PBS alone was administered intraperitoneally at 8 hours after LPS injection. Twenty-four hours after LPS injection, the aqueous humor of the right eyes was collected for leukocyte count, protein concentration, and IL-6 assay. Left eyes were processed for routine histology.
  
  
  RESULTS
  TGF beta-1-treated mice showed less ocular inflammation histologically than to the animals that were given PBS. This was confirmed by decreases in leukocyte count, protein concentration, and IL-6 level in the aqueous humor.
  
  
  CONCLUSIONS
  TGF beta-1 inhibits the development of EIU. TGF beta-1 may be useful for the modulation of uveitis in humans.
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
authors.forename: B
authors.initials: B
authors.lastname: Peng
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: F G
authors.initials: FG
authors.lastname: Roberge
authors.forename: C C
authors.initials: CC
authors.lastname: Chan
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000276:immunology; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D012486:Salmonella typhimurium; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014606:Uveitis, Anterior / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D004731:Endotoxins; D016212:Transforming Growth Factor beta