Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization.
Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.
Rosenberg
C
C
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. carla@ruly46.leidenuniv.ni
Voltz
A K
AK
Lawler
A M
AM
Lamb
B T
BT
Stetten
G
G
Gearhart
J D
JD
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008822:Mice, Transgenic; D015183:Restriction Mapping; D013234:Stem Cells; D013482:Superoxide Dismutase / Q000235:genetics; D014162:Transfection; D019076:Transgenes
D002843:Chromatin; D013482:Superoxide Dismutase