Effects of experimental dicrocoeliosis on oxidative drug metabolism in hamster liver.
The purpose of this investigation was to determine the effects of experimental dicrocoeliosis on the hepatic oxidative drug-metabolizing system in hamsters. Studies were carried out 80 and 120 days after infestation with an oral dose of 40 metacercariae of Dicrocoelium dendriticum. The parasitic pathology was ascertained by detection of the fluke eggs in faeces, increased serum alanine aminotransferase and aspartate aminotransferase activities, and postmortem and histological findings. Cytochrome P-450 concentration, aniline hydroxylase activity and ethoxycoumarin O-deethylase activity were significantly decreased in both groups of infected animals. Aminopyrine N-demethylase activity and erythromycin N-demethylase activity were only reduced 120 days after infection. Effects on drug metabolizing enzymes were unrelated to changes in the physical state of the microsomal membrane, as assessed by measurement of fluorescence polarization. The results of this study indicate that the capacity of the liver for handling drugs and xenobiotics may be impaired as a consequence of dicrocoeliosis.
Sánchez-Campos
S
S
Department of Physiology, Pharmacology and Toxicology, University of Leon, Spain.
Tuñón
M J
MJ
González
P
P
Campo
R
R
Ferreras
M C
MC
Manga
Y
Y
González-Gallego
J
J
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000704:Analysis of Variance; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001219:Aspartate Aminotransferases / Q000097:blood; D006224:Cricetinae; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004011:Dicrocoeliasis / Q000503:physiopathology*; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005454:Fluorescence Polarization; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D014200:Trematoda / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase; D000815:Aniline Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D000633:Aminopyrine N-Demethylase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase