Induction of tubulogenesis of microvascular endothelial cells by basic fibroblast growth factor from human SN12C renal cancer cells.
OBJECTIVE
To investigate the role of basic fibroblast growth factor (b-FGF) in angiogenesis in renal cell carcinoma.


METHODS
We examined b-FGF expression in 25 patients with renal cell carcinoma (RCC) by reverse transcriptase polymerase chain reaction (RT-PCR). We measured the tube formation by human omental microvascular endothelial (HOME) cells induced by b-FGF in a three-dimensional collagen gel in the absence or presence of SN12C human renal cancer cells by means of computer-image analysis.


RESULTS
RT-PCR analysis detected b-FGF m-RNA in 20 (80%) of 25 patients with RCC. The tube formation of HOME cells was increased in the presence of SN12C cells overexpressing b-FGF mRNA, induced by exogenous b-FGF in a dose dependent manner and completely inhibited by anti-b-FGF antibody.


CONCLUSIONS
Basic-FGF may develop tumor angiogenesis by inducing microvessel tubulogenesis in patients with RCC.
Emoto
A
A
Department of Urology and Neurosurgery, Oita Medical University, Japan.
Nakagawa
M
M
Wakabayashi
Y
Y
Hanada
T
T
Naito
S
S
Nomura
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002196:Capillaries / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000098:blood supply* / Q000378:metabolism*; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D014407:Tumor Cells, Cultured
D016222:Fibroblast Growth Factor 2