Effects of 5-hydroxytryptamine agonists on myoelectric activity of the forestomach and antroduodenal area in sheep.
To increase knowledge of the role of 5-hydroxytryptamine (5-HT) receptors in the regulation of reticuloruminal, omasal and antroduodenal myoelectric activity in sheep, the effects of 5-HT agonists on forestomach and antroduodenal myoelectric activity have been investigated in conscious sheep. 5-Carboxamidotryptamine, methysergide, alpha-methyl-5-HT, 2-methyl-5-HT, cisapride, zacopride or metoclopramide were infused intravenously for 5 min and myoelectric recordings were obtained from electrodes chronically implanted in the reticulum, rumen (dorsal sac), omasal body, abomasal antrum and duodenal bulb. The integrated activity of the reticular and ruminal spike bursts was modified only by the highest doses of alpha-methyl-5-HT, 2-methyl-5-HT, metoclopramide and cisapride. A phase III-like activity pattern was recorded in the antroduodenal area with all 5-HT-ergic agents and a dose-dependent inhibition of myoelectric activity was recorded in both reticulorumen and omasum at the same time as the antroduodenal effects. In the forestomach, methysergide alone induced inhibition of ruminal secondary contractions; 5-HT, alpha-methyl-5-HT, cisapride and metoclopramide, moreover, evoked an initial dose-dependent increase in antral activity. These results suggest that 5-HT1, 5-HT2, 5-HT3 and 5-HT4 receptors are involved in the regulation of the migrating myoelectric complex in sheep and in the genesis of forestomach hypomotility that is occasionally recorded concomitantly with the spontaneous duodenal phase III in sheep. 5-HT4 receptors also have a prokinetic action in the antral area.
Plaza
M A
MA
Department of Pharmacology and Physiology, Veterinary Faculty, Zaragoza, Spain.
Arruebo
M P
MP
Murillo
M D
MD
D000818:Animals; D004386:Duodenum / Q000187:drug effects / Q000502:physiology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects*; D011985:Receptors, Serotonin / Q000502:physiology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D012756:Sheep; D013270:Stomach / Q000187:drug effects / Q000502:physiology
D011985:Receptors, Serotonin; D017366:Serotonin Receptor Agonists