[Acrylonitrile and chemotaxis: in vitro experiments].
We have verified that chemotaxis of isolated human polymorphonuclear leukocytes (PMN) was a target of in vitro toxicant effect of acrylonitrile (ACN). This toxicant induced a significant dose dependent decreasing of chemotaxis with 50% inhibition (IC50) occurring at 15 mM. We assume that PMN from workers exposed to ACN reacts vivo in a similar way to PMN exposed in vitro to ACN. We propose therefore to use chemotaxis assay as a biomarker of early biological effect of ACN in workers, since for their monitoring there are so far only internal dose indicators but no suitable effect indicators.
Monaco
F
F
Clinica di Medicina del Lavoro, Università di Ancona, Ospedale. Regionale.
Amati
M
M
Visonà
I
I
D000181:Acrylonitrile / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D000328:Adult; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003545:Cysteine / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*
D003545:Cysteine; D000181:Acrylonitrile