Typing of verotoxins by DNA colony hybridization with poly- and oligonucleotide probes, a bead-enzyme-linked immunosorbent assay, and polymerase chain reaction.
To identify the type of Verotoxins (VT) produced by Verocytotoxin-producing Escherichia coli (VTEC), a sensitive bead-enzyme-linked immunosorbent assay and polymerase chain reaction with common and specific primers to various VTs (VT1, VT2, VT2vha, VT2vhb, and VT2vp1) were developed. Together with colony hybridization tests with oligo- and polynucleotide probes, these methods were applied to VTEC isolates to type the VT produced. The toxin types of 26 of 37 strains were identified, but the reaction profiles in assays of the remaining 11 strains suggested the existence of new VT2 variants. The application of these identification procedures may be useful as a tool for clinical and epidemiological studies of VTEC infection.
Yamasaki
S
S
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
Lin
Z
Z
Shirai
H
H
Terai
A
A
Oku
Y
Y
Ito
H
H
Ohmura
M
M
Karasawa
T
T
Tsukamoto
T
T
Kurazono
H
H
Takeda
Y
Y
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000145:classification*; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000096:biosynthesis / Q000145:classification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007164:Immunosorbents; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011119:Polynucleotides; D012703:Serotyping / Q000379:methods; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D007164:Immunosorbents; D015345:Oligonucleotide Probes; D011119:Polynucleotides; D022622:Shiga Toxin 1