title: [Synthesis, cloning, and expression in Escherichia coli cells of human alpha1-antitrypsin cDNA].
abstract: cDNA coding for the full-length human alpha 1-antitrypsin (AAT) and its leader sequence has been cloned and sequenced. DNA sequences encoding the deletion variants and a full-length copy of AAT were cloned and expressed under trp-promoter control. It has been shown that guanine replacing right upstream and downstream the ATG of deletion variant increases the expression to ten-fold. The synthesis of deletion AAT in E. coli was evaluated immunologically and the level of the synthesis was shown to be 4-5% of total cellular protein.
authors.forename: N V
authors.initials: NV
authors.lastname: Tikunova
authors.forename: S Ia
authors.initials: SIa
authors.lastname: Golovin
authors.forename: N N
authors.initials: NN
authors.lastname: Mikriukov
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D000515:alpha 1-Antitrypsin