Segregation of yeast polymorphic STA genes in meiotic recombinants and analysis of glucoamylase production.
Hybrid yeast strains were constructed using haploid Saccharomyces cerevisiae and Saccharomyces cerevisiae var. diastaticus strains to get haploid meiotic recombinants having more than one copy of STA1, STA2, and STA3 genes. STA genes were localized on the chromosomes by pulsed field gel electrophoresis. Working gene dosage effects were found among STA genes in liquid starch medium, indicating low levels of glucose repression. Growth of strains, however, was not influenced by their STA copy number.
Balogh
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Department of Microbiology and Biotechnology, University of Horticulture and Food Industry, Budapest, Hungary. h11636mar@ella.hu
Maráz
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D015825:Chromosomes, Fungal; D003433:Crosses, Genetic; D003470:Culture Media; D016521:Electrophoresis, Gel, Pulsed-Field; D018628:Gene Dosage; D005800:Genes, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D006238:Haploidy; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008540:Meiosis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013213:Starch / Q000378:metabolism
D003470:Culture Media; D007527:Isoenzymes; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase