Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.
A cDNA encoding a subunit of the verrucotoxin (VTX) has been identified from a cDNA library derived from stonefish venom glands. It encodes a polypeptide of 708 amino-acid residues, followed by a 3'-untranslated region of 895 bp long. The ORF contains the complete mature sequence of the beta-subunit of the VTX, as inferred from both the presence of an identical N-terminus sequence and 96% homology among the 506 amino terminus residues found in the partial sequence of the beta-subunit of the stonustoxin from Synanceia horrida (Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., and Yuen, R. (1994) Toxicon 32, 1684-1688). Upstream the mature sequence, we noticed the presence of an incomplete peptide of a 13 amino acids, whose unusual primary structure supports the idea of the existence of a propeptide and/or of a new secretion signal.
Garnier
P
P
Laboratoire de Biologie et Contrôle des Organismes parasites (Equipe Toxines et Insectes), Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
Ducancel
F
F
Ogawa
T
T
Boulain
J C
JC
Goudey-Perrière
F
F
Perrière
C
C
Ménez
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005397:Fish Venoms / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D005397:Fish Venoms; D006023:Glycoproteins; C094408:verrucotoxin protein, Synanceia verrucosa