Exogenous pulses of luteinizing hormone cause persistence of the largest bovine ovarian follicle.
The objectives of this study were, 1) to determine whether increased frequency of LH pulses, independent of changes in progesterone, will cause persistence of the first largest ovarian follicle (LF1) during the bovine estrous cycle and 2) to determine whether a follicle produced in this manner will ovulate after luteal regression. In Exp. 1, 32 nonlactating beef cows were randomized into four groups. Bovine LH was given as 50 micrograms i.v. every 2 h from 0800 on d 7 through 0800 on d 13 postestrus to cows in two groups, which in addition either received PGF2 alpha on d 12 and were allowed to ovulate (LHOV; Group 1) or were ovariectomized (LHOVX; Group 2) on d 13. Cows in Group 3 received progesterone via a used controlled internal drug releasing device (CIDR) on d 4 to 13, PGF2 alpha on d 6, and ovariectomy on d 13. These cows received 1 mL of saline i.v. every 2 h on d 7 to 13, as did those in Group 4 (SAL), but cows in Group 4 did not receive any PGF2 alpha. Proportion (percentage) of LF1 maintained until d 13 was similar in cows with CIDR (100) and on LH (88) but less in cows on SAL (36; P < .01). The LF1 were largest in cows with CIDR, smallest in cows on SAL, and intermediate in cows on LH (P < .001). During d 7 to 13, progesterone was lesser and estradiol was greater in peripheral plasma in cows with CIDR than in cows on LH or SAL (P < .001). In Experiment 2, beef heifers received 50 micrograms of bLH (LH; n = 5) or saline (SAL; n = 4) i.v. every 2 h from 0800 on d 7 until 12 h after signs of estrus were observed following PGF2 alpha on d 12. The LF1 were maintained by LH and 60% ovulated; all LF1 were replaced during treatment in heifers on SAL. The LF1 were larger in heifers on LH, but estradiol and progesterone did not differ between groups. In conclusion, an increased frequency of LH pulses during a normal luteal phase allowed LF1 to persist but did not increase secretion of estradiol.
Taft
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Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108, USA.
Ahmad
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Inskeep
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D000818:Animals; D002417:Cattle / Q000097:blood / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood / Q000502:physiology; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D008183:Luteal Phase / Q000187:drug effects / Q000502:physiology; D007986:Luteinizing Hormone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010052:Ovariectomy; D010060:Ovulation / Q000187:drug effects / Q000502:physiology; D011374:Progesterone / Q000097:blood / Q000502:physiology; D011863:Radioimmunoassay / Q000662:veterinary; D012044:Regression Analysis; D013997:Time Factors
D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D015237:Dinoprost