Bacterial protease Lon is a site-specific DNA-binding protein.
The product of the Escherichia coli lon gene is the ATP-dependent Lon protease. Lon contributes to the regulation of several important cellular functions, including radiation resistance, cell division, filamentation, capsular polysaccharide production, lysogeny of certain bacteriophages, and proteolytic degradation of certain regulatory and abnormal proteins. Lon homologues are also found in several widely divergent bacteria, as well as in the mitochondria of yeast and humans. E. coli Lon has long been known to bind to DNA, but this interaction has not been further characterized and has generally been assumed to be nonspecific. We now demonstrate that E. coli Lon can bind to a TG-rich DNA promoter element in a sequence-specific manner. This finding is based on the results of experiments employing SouthWestern blotting, protein purification, "shift-shift" electrophoretic mobility shift assays, electrophoretic mobility shift assays using in vitro transcribed and translated Lon, and DNase footprinting. Site-specific DNA binding is likely to be an additional important biochemical characteristic of the multifaceted Lon protease.
Fu
G K
GK
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0642, USA.
Smith
M J
MJ
Markovitz
D M
DM
D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D015498:HIV-2 / Q000235:genetics; D006360:Heat-Shock Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D049070:Protease La; D011994:Recombinant Proteins; D012697:Serine Endopeptidases / Q000737:chemistry*
D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La