A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences.
A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.
Blais
B W
BW
Laboratory Services Division, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
Turner
G
G
Sooknanan
R
R
Malek
L T
LT
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology; D004531:Eggs / Q000382:microbiology; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D012329:RNA, Bacterial; D012333:RNA, Messenger