Cloning of a Bacillus sp. BP-23 gene encoding a xylanase with high activity against aryl xylosides.
The xynB gene encoding a xylanase from the recently isolated Bacillus sp. strain BP-23 has been cloned and expressed in Escherichia coli. The enzyme produced in this host shows a molecular size of 41 kDa and a pI of 4.5. The pH and temperature at which the highest activity was found were 5.5 and 50 degrees C respectively. Crude xylanase B showed activity on xylan, aryl xylosides, xylotetraose and xylotriose, while xylobiose was not hydrolyzed by the enzyme. Xylanase B showed high specific activity on aryl xylosides, probably as a result of the transxylosidase activity detected.
Blanco
A
A
Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
Díaz
P
P
Martínez
J
J
López
O
O
Soler
C
C
Pastor
F I
FI
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006027:Glycosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D015183:Restriction Mapping; D013379:Substrate Specificity; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D006027:Glycosides; C010807:xylosides; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase