Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid.
Samples of oral fluid collected from 18 patients seropositive for hepatitis C virus (HCV) and 49 seronegative patients were tested for the presence of HCV antibodies with two modified serum-screening kits. The following sensitivities and specificities were obtained: HCV 3.0 assay, 72% and 98%, respectively, and Monolisa anti-HCV assay, 100%, and 100%, respectively. The modified Monolisa assay demonstrated a striking concordance between serum and saliva samples. The use of oral fluids offers a convenient and noninvasive method applicable to HCV epidemiological studies and screening of high-risk groups.
McIntyre
P G
PG
Department of Medical, Microbiology, Ninewells Hospital, Dundee, UK.
Laszlo
J
J
Appleyard
K
K
Ogden
G R
GR
D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D012016:Reference Values; D012463:Saliva / Q000821:virology; D012680:Sensitivity and Specificity
D018937:Hepatitis C Antibodies