Proteolytic processing of Chlorella virus CVK2 capsid proteins.
Chlorella virus CVK2 particles contain several capsid proteins that are selectively released by treatment with 4 M urea. Seven of them (Vp52, Vp45, Vp41, Vp25, Vp20.5, Vp20, and Vp16.2) have been identified based on amino acid sequences at the N-terminus. Comparison of the amino acid sequences with the corresponding ORFs deduced from the nucleotide sequence of PBCV-1, the prototype member of Phycodnaviridae, revealed that the CVK2 proteins were processed at the N-terminal region by two different proteolytic activities.
Songsri
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Faculty of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739, Japan.
Hiramatsu
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Fujie
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Yamada
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D000595:Amino Acid Sequence; D002213:Capsid / Q000378:metabolism*; D002708:Chlorella / Q000821:virology*; D010450:Endopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017862:Phycodnaviridae / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D010450:Endopeptidases