title: Serologic markers in early stages of African horse sickness virus infection.
abstract: Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection.
authors.affiliation: INGENASA, Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Martínez-Torrecuadrada
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Laviada
authors.forename: P
authors.initials: P
authors.lastname: Roy
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: C
authors.initials: C
authors.lastname: Vela
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
authors.forename: J I
authors.initials: JI
authors.lastname: Casal
mesh_terms: D000355:African Horse Sickness / Q000276:immunology*; D000356:African Horse Sickness Virus / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D014611:Vaccination; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus; D014764:Viral Proteins; D014765:Viral Vaccines