title: A CK2 site is reversibly phosphorylated in the photosystem II subunit CP29.
abstract: Protein phosphorylation is a major mechanism in the regulation of protein function. In chloroplast thylakoids several photosystem II subunits, including the major antenna light-harvesting complex II and several core complex components, are reversibly phosphorylated depending on the redox state of the electron carriers. A previously unknown reversible phosphorylation event has recently been described on the CP29 subunit which leads to conformational changes and protection from cold stress (Bergantino, E., Dainese, P., Cerovic, Z. Sechi, S. and Bassi, R. (1995) J. Biol Chem. 270, 8474-8481). In this study, we have identified the phosphorylation site on the N-terminal, stroma-exposed domain, showing that it is located in a sequence not homologous to the other members of the Lhc family. The phosphorylated sequence is unique in chloroplast membranes since it meets the requirements for CK2 (casein kinase II) kinases. The possibility that this phosphorylation is involved in a signal transduction pathway is discussed.
authors.affiliation: Università di Verona, Facoltà di Scienze MM.FF.NN., Biotecnologie Vegetali, Verona, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Testi
authors.forename: R
authors.initials: R
authors.lastname: Croce
authors.forename: P
authors.initials: P
authors.lastname: Polverino-De Laureto
authors.forename: R
authors.initials: R
authors.lastname: Bassi
mesh_terms: D000595:Amino Acid Sequence; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: C069660:CP29 light harvesting complex; D045342:Light-Harvesting Protein Complexes; D010761:Phosphorus Radioisotopes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D011994:Recombinant Proteins