title: [Utilization of nylon membranes for specific isolation and characterization of verotoxin-producing Escherichia coli using DNA probes].
abstract: A method for specific isolation of VT(+)-strains in raw milk is given. DNA-hybridization technique with DIG-labeled PCR-amplificates as probes are the basis. No background is seen by using "DIG Easy Hyb" solution and nylon membranes for colony- and plaque-hybridization (Boehringer Mannheim GmbH). Marked colonies are visible on the membranes after detection. So it is possible to select these colonies from a masterplate. The results are available within one day (without enrichment and membrane preparation). After stripping the membranes can be used for a new hybridisation to detect another factor of virulence.
authors.affiliation: Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Standorte in Dessau.
authors.forename: P
authors.initials: P
authors.lastname: Gallien
authors.forename: H
authors.initials: H
authors.lastname: Klie
authors.forename: K W
authors.initials: KW
authors.lastname: Perlberg
authors.forename: D
authors.initials: D
authors.lastname: Protz
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D015342:DNA Probes; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D008567:Membranes, Artificial; D008892:Milk / Q000382:microbiology*; D009757:Nylons; D016133:Polymerase Chain Reaction / Q000379:methods; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D008567:Membranes, Artificial; D009757:Nylons; D022622:Shiga Toxin 1