An epidemiological study of Salmonella enteritidis by pulsed-field gel electrophoresis (PFGE): several PFGE patterns observed in isolates from a food poisoning outbreak.
An epidemiological analysis of Salmonella enteritidis from a food poisoning was done using pulsed-field gel electrophoresis (PFGE) of BlnI- or XbaI-digested fragments of chromosomal DNA of isolates. S. enteritidis isolates obtained from 19 patients had identical PFGE patterns. Therefore, a strain giving the same pattern was considered to be the causative agent of this outbreak. In addition, four isolates that had different BlnI-digested PFGE patterns were obtained from three patients, suggesting that the observed variations in PFGE patterns might occur as the result of some point mutations of chromosomal DNA during growth or from the existence of several S. enteritidis strains from various sources. Subsequent PFGE analysis of continuously subcultured strains supported the former possibility. These observations indicate that PFGE analysis on multiple numbers of colonies from each patient are necessary for the epidemiologic investigation of S. enteritidis.
Murase
T
T
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Japan.
Nakamura
A
A
Matsushima
A
A
Yamai
S
S
D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017720:Molecular Epidemiology; D017354:Point Mutation; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific