Purification and characterization of a lectin from Amaranthus hypochondriacus var. Mexico seeds.
A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.
Ozeki
M
M
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.
Kamemura
K
K
Moriyama
K
K
Itoh
Y
Y
Furuichi
Y
Y
Umekawa
H
H
Takahashi
T
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006241:Haptens / Q000032:analysis; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D006241:Haptens; D037102:Lectins; D037121:Plant Lectins