title: Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase with activity against N-acetylglucosamine from Vibrio cholerae non-O1.
abstract: An enzyme that deacetylates N-acetylglucosamine to glucosamine from Vibrio cholerae non-O1 was purified to homogeneity by sequential procedures. The native enzyme had a molecular mass of 190,000 Da and was predicted to be composed of four identical subunits with molecular masses of 45,000 Da. The purified enzyme hydrolyzed N-acetylglucosamine, N-acetylglucosamine 6-phosphate, and N-acetylglucosamine 6-sulfate, but not chitin oligosaccharides, and N-acetylgalactosamine. The deacetylase activity was completely abolished by N-ethylmaleimide, p-chloromercuribenzoate, EDTA, and Cu2+. On the other hand, the activity was activated by Co2+. The amino-terminal amino acids of the purified enzyme were sequenced. Among the 22 N-terminal amino acid residues, 12 residues of Vibrio deacetylase were identical with that of Escherichia coli GlcNAc 6-phosphate deacetylase.
authors.affiliation: Osaka National Research Institute, Agency of Industrial Science and Technology, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamano
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushita
authors.forename: Y
authors.initials: Y
authors.lastname: Kamada
authors.forename: S
authors.initials: S
authors.lastname: Fujishima
authors.forename: M
authors.initials: M
authors.lastname: Arita
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D000581:Amidohydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D008670:Metals; D000581:Amidohydrolases; C018717:N-acetylglucosamine-6-phosphate deacetylase; D000117:Acetylglucosamine