Ty1-mediated integration of expression cassettes: host strain effects, stability, and product synthesis.
The yeast retrotransposon Ty1 has been used to insert multiple copies of heterologous genes into the genome of Saccharomyces cerevisiae. Amplification using a GAL1-regulated Ty1 element carrying a 4.6 kilobase pair expression cassette (Escherichia coli lacZ structural gene under the control of the yeast CUP1 promoter, and the bacterial neo gene) was compared with that of a GAL1-regulated Ty1 element carrying only the neo gene. Mobilization of Ty1 was induced from a chromosomal element and a 2 mu-plasmid-based element; similar results were obtained for both locations. The two marked Ty1 cassettes were successfully integrated into the genomes of three different S. cerevisiae strains. Efficiencies were found to vary significantly between strains. The size of the inserted cassette was also important; the efficiency for the CUP1p-lacZ-neo cassette was much lower than that for the neo cassette in the same host. All amplified copies were found to be quite stable with or without expression for at least 50 generation in nonselective medium; moreover, there were no significant effects on the growth of the cells. After integration, beta-galactosidase specific activity from the lacZ construct in the three hosts was found to correlate well with the copy number of the CUP1p-lacZ expression cassette amplified by the Ty1 retrotransposon.
Lee
F W
FW
Department of Chemical and Biochemical Engineering, University of California, Irvine 92717-2575, USA.
Da Silva
N A
NA
D015139:Blotting, Southern; D015825:Chromosomes, Fungal / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media; D004251:DNA Transposable Elements / Q000235:genetics*; D005784:Gene Amplification; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D007763:Lac Operon; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D003470:Culture Media; D004251:DNA Transposable Elements; D001616:beta-Galactosidase