title: Studies on the hypomethylation of c-myc, c-Ha-ras oncogenes and histopathological changes in human gastric carcinoma.
abstract: In order to study the status of DNA methylation of specific oncogenes and the relationship between them and the pathological changes in gastric carcinoma, we analysed the methylated status of c-myc, c-Ha-ras oncogenes by Southern blot hybridization. Genomic DNA from cancerous, paracancerous and non-cancerous areas of surgically resected specimens were examined in 22 cases of advanced human gastric carcinoma. Specimens were digested by the restriction endonucleases MspI/HpaII, which are able to cleave between methylated and non-methylated cytosine at their nucleotide recognition site the DNA 5'-CCGG sequence, and were hybridized with c-myc, c-Ha-ras oncogene probes. Moreover, the corresponding pathological changes in gastric carcinoma were observed. The results showed that c-myc, c-Ha-ras oncogenes from cancerous (10/22, 5/10) and paracancerous areas (13/22, 4/10) were hypomethylated and that there was no significant relationship between them and the histopathological changes.
authors.affiliation: Shanghai Second Medical University Affiliated Ren-Ji Hospital, Shanghai Institute of Digestive Disease, People's Republic of China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Fang
authors.forename: S S
authors.initials: SS
authors.lastname: Zhu
authors.forename: S D
authors.initials: SD
authors.lastname: Xiao
authors.forename: S J
authors.initials: SJ
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: X Y
authors.initials: XY
authors.lastname: Chen
authors.forename: X M
authors.initials: XM
authors.lastname: Zhou
authors.forename: L F
authors.initials: LF
authors.lastname: Qian
mesh_terms: D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D008297:Male; D008745:Methylation; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*