title: Rapid detection and isolation of DNA-binding compounds from Streptomyces xanthochromogenes.
abstract: The culture medium of Streptomyces xanthochromogenes JH903 was found to show selective activity against DNA-repair-deficient Escherichia coli CM871 strain. In this report we describe a simple method to locate and isolate DNA binding compounds from the fermentation broth. The method is based on the retention of DNA-reacting compounds in cellulose complexed with DNA, and purification of these compounds with thin-layer chromatography. Screening of microbial metabolites from chloroform extracts of fermentation media resulted in detection of five genotoxic fractions.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Holmalahti
authors.forename: S
authors.initials: S
authors.lastname: Junttila
authors.forename: A
authors.initials: A
authors.lastname: von Wright
mesh_terms: D004247:DNA / Q000378:metabolism*; D005285:Fermentation; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D004247:DNA