Purification of transforming growth factor beta 1 from human platelets.
Transforming growth factor-beta (TGF beta) is a growth and differentiation factor which can be released from many cell types. In previous studies, platelets were identified as a rich source of TGF beta. Here we present a rapid and convenient method for TGF beta purification from human platelets which includes acid-ethanol extraction and gelfiltration, cation exchange and reversed phase chromatography. All purification steps are performed under acidic conditions to prevent adsorption of TGF beta to the vial walls. In addition, volatile solvents and buffers were used which allowed easy removal of solvent and salt by lyophilization. Using this method pure TGF beta can be easily obtained in high yield (370 micrograms) from 20 units of platelet concentrate.
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Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe-University Frankfurt, Germany.
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Steinhilber
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D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis; D001681:Biological Assay; D001792:Blood Platelets / Q000737:chemistry*; D015153:Blotting, Western; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003864:Depression, Chemical; D006801:Humans; D016212:Transforming Growth Factor beta / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D001094:Arachidonate 5-Lipoxygenase