Increased expression of interleukin-1 receptors on fibroblasts derived from inflamed gingiva.
We investigated the expression of interleukin-1 receptors (IL-1R) on the surfaces of cultured gingival fibroblasts derived from healthy and inflamed gingiva and the effects of IL-1 alpha and IL-1 beta and prostaglandin E2 (PGE2) on IL-1R expression. Fibroblasts were obtained from explant culture of both healthy and inflamed gingiva. IL-1R on cell surfaces was detected immunohistochemically using an anti-human IL-1R monoclonal antibody. IL-1R expression was assessed quantitatively using an enzyme linked immunosorbent assay (ELISA). Positive staining for IL-1R was more evident on cells from inflamed gingiva compared with cells from healthy gingiva. The ELISA showed a significantly increased number of IL-1R on cells from inflamed gingiva compared with cells from healthy gingiva (P < 0.05). Treatment with IL-1 but not PGE2 increased expression of IL-1R on fibroblasts. These findings suggest that gingival fibroblast responses to IL-1 may represent a mechanism for amplification of gingival inflammation.
Kanda-Nakamura
C
C
Department of Periodontology, Kagoshima University Dental School, Japan.
Izumi
Y
Y
Sueda
T
T
D000328:Adult; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005891:Gingivitis / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*
D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D015232:Dinoprostone