title: The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.
abstract: The transcription initiation factor TFIID is a multimeric protein complex composed of TATA box-binding protein (TBP) and many TBP-associated factors (TAF(II)s). TAF(II)s are important cofactors that mediate activated transcription by providing interaction sites for distinct activators. Here, we present evidence that human TAF(II)250 and its homologs in Drosophila and yeast have histone acetyltransferase (HAT) activity in vitro. HAT activity maps to the central, most conserved portion of dTAF(II)230 and yTAF(II)130. The HAT activity of dTAF(II)230 resembles that of yeast and human GCN5 in that it is specific for histones H3 and H4 in vitro. Our findings suggest that targeted histone acetylation at specific promoters by TAF(II)250 may be involved in mechanisms by which TFIID gains access to transcriptionally repressed chromatin.
authors.affiliation: Department of Biology, University of Rochester, New York 14627, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Mizzen
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Kokubo
authors.forename: J E
authors.initials: JE
authors.lastname: Brownell
authors.forename: A J
authors.initials: AJ
authors.lastname: Bannister
authors.forename: T
authors.initials: T
authors.lastname: Owen-Hughes
authors.forename: J
authors.initials: J
authors.lastname: Workman
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: S L
authors.initials: SL
authors.lastname: Berger
authors.forename: T
authors.initials: T
authors.lastname: Kouzarides
authors.forename: Y
authors.initials: Y
authors.lastname: Nakatani
authors.forename: C D
authors.initials: CD
authors.lastname: Allis
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D004268:DNA-Binding Proteins; D004331:Drosophila melanogaster; D005656:Fungal Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D019476:Insect Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D010455:Peptides; D011494:Protein Kinases; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor