The petunia homologue of tomato gast1: transcript accumulation coincides with gibberellin-induced corolla cell elongation.
Gibberellins (GAs) regulate petunia corolla pigmentation and elongation. To study this hormone's effect at the molecular level, we used the tomato gast1 gene as a probe to isolate a gibberellin-induced gene (gip) from petunia corollas. The deduced sequence of gip exhibited 82% identity with GAST1 protein and contained a short, highly hydrophobic N-terminal region. High levels of gip expression were detected in elongating corollas and young stem intemodes. When detached corollas were grown in vitro in sucrose medium, gip expression was strongly induced by gibberellic acid (GA3). GA3-induced gip expression in corollas was inhibited by abscisic acid (ABA). The expression of the gene was also induced by GA3 in detached young stem segments. Sucrose was not essential for GA-induced gip expression in corollas but enhanced its effect. In stems, on the other hand, sucrose inhibited the effect of the hormone. The results of the present work support the possible role of gip in GA-induced corolla and stem elongation.
Ben-Nissan
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The Kennedy-Leigh Centre for Horticultural Research, The Hebrew University of Jerusalem, Israel.
Weiss
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D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005875:Gibberellins / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000235:genetics; D008969:Molecular Sequence Data; D059828:Plant Cells; D063245:Plant Development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013395:Sucrose / Q000494:pharmacology
D003470:Culture Media; C081148:GAST1 protein, Lycopersicon esculentum; D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C103693:gip1protein, Petunia; D013395:Sucrose; D000040:Abscisic Acid; C007842:gibberellic acid