title: Structure and transcription of the gene for translation elongation factor 1 subunit alpha of zebrafish (Danio rerio).
abstract: The zebrafish gene for translation elongation factor 1 alpha (EF1 alpha) was isolated from a phage Lambda genomic library and sequence and structure determined. One gene copy of EF1 alpha per haploid set of chromosomes was found and no processed pseudogenes. A highly active promoter region was localized to a 277 bp PstI/PvuII fragment beginning 240 bp upstream from the tsp, but no transcription enhancing, or silencing activity was observed within 1 kbp upstream, or downstream from the promoter. Expression of EF1 alpha appears to be developmentally regulated.
authors.affiliation: Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455, USA.
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: T
authors.initials: T
authors.lastname: Murphy
authors.forename: W
authors.initials: W
authors.lastname: Sauerbier
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D004625:Embryo, Nonmammalian / Q000502:physiology; D005091:Exons; D005306:Fertilization; D018507:Gene Expression Regulation, Developmental; D015698:Genomic Library; D006238:Haploidy; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015027:Zebrafish / Q000235:genetics*
chemical_list: D017931:DNA Primers; D046911:Macromolecular Substances; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors