Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells.
We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10(-9) -10(-4) M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.
Gardner
T W
TW
Ulerich Ophthalmology Research Laboratory, Department of Ophthalmology, Penn State University College of Medicine, Hershey, PA 17033, USA.
Lesher
T
T
Khin
S
S
Vu
C
C
Barber
A J
AJ
Brennan
W A
WA
D000818:Animals; D002199:Capillary Permeability / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000235:genetics; D051272:Glucose Transporter Type 1; D006632:Histamine / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D019108:Tight Junctions / Q000737:chemistry / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein
D051272:Glucose Transporter Type 1; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D010750:Phosphoproteins; D011968:Receptors, Histamine; D062826:Zonula Occludens-1 Protein; D006632:Histamine