Migration of neurons containing gonadotropin releasing hormone (GnRH) in slices from embryonic nasal compartment and forebrain.
During development, neurons containing gonadotropin-releasing hormone traverse fiber bundles in the nose, cross into the brain, and move through a maze of glial and axonal fibers. To test whether GnRH neurons utilize cues intrinsic to their migration route to traverse the nasal/brain boundary, tissue slices that maintain connections between the forebrain and nasal compartment were prepared from mouse embryos. Cell migration between the nasal and brain compartments was evident based on changes in cell positions after successive days in vitro.
Tobet
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SA
Department of Biomedical Sciences, Shriver Center, Waltham, MA 02254, USA. stobet@shriver.org
Hanna
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Schwarting
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GA
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology; D005314:Embryonic and Fetal Development / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D009833:Olfactory Pathways / Q000737:chemistry* / Q000166:cytology / Q000196:embryology; D016548:Prosencephalon / Q000737:chemistry* / Q000166:cytology / Q000196:embryology
D016895:Culture Media, Serum-Free; D007987:Gonadotropin-Releasing Hormone