Gene analysis of trehalose-producing enzymes from hyperthermophilic archaea in Sulfolobales.
The genes encoding new trehalose-producing enzymes from S. acidocaldarius ATCC33909 were cloned to analyze the distribution of these genes in Sulfolobales. Comparison of the amino acid sequences with S. solfataricus KM1 showed approximately 50% similarity. Southern analysis suggest that homologues of the trehalose-producing enzyme genes exist widely in sulfolobales and strains in Sulfolobales were classified into three kinds of genotypes.
Kobayashi
K
K
Applied Bioresearch Center, Kirin Brewery Co., Ltd., Gunma, Japan.
Kato
M
M
Miura
Y
Y
Kettoku
M
M
Komeda
T
T
Iwamatsu
A
A
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005815:Genetic Code; D016695:Glycosyltransferases / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016942:Sulfolobales / Q000201:enzymology / Q000235:genetics*; D014199:Trehalose / Q000096:biosynthesis*; D000516:alpha-Amylases / Q000235:genetics*
D014199:Trehalose; D016695:Glycosyltransferases; D000516:alpha-Amylases