PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures.
The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.
Valente
P
P
Instituto de Microbiologia Prof. Paulo de Goes, CCS, UFRJ Brazil.
Gouveia
F C
FC
de Lemos
G A
GA
Pimentel
D
D
van Elsas
J D
JD
Mendonça-Hagler
L C
LC
Hagler
A N
AN
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012440:Saccharomyces / Q000145:classification / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal