Instability of chromosome 6 microsatellite repeats in human cervical tumors carrying papillomavirus sequences.
Loss of heterozygosity (LOH) in chromosome 6 in human squamous cervical carcinomas was analyzed in the long and short arms of the chromosome using 3 pairs of primers each. In all cases, normal adjacent tissue was used as control. Among 51 cases analyzed, we identified LOH or microsatellite instability in 23% using primer D6S291 (located at position 6p21.3) and in 11% using primers D6S308 (6q16.3-6q27) and D6S270 (6q22.3-6q23.2). On the contrary, no significant LOH or genomic instabilities were detected with primers D6S306 (6p22.3-6p21.2), D6S299 (6p22.3-6p21.3) and D6S287 (6q21-6q23.3). Our results thus suggest the existence of instable loci at 3 regions of chromosome 6. Whether these loci contain putative tumor-suppressor genes or genes involved in cell cycle control remains unknown.
Kisseljov
F
F
Department of Viral Molecular Biology, Kazan Cancer Research Center, Moscow, Russia.
Semionova
L
L
Samoylova
E
E
Mazurenko
N
N
Komissarova
E
E
Zourbitskaya
V
V
Gritzko
T
T
Kozachenko
V
V
Netchushkin
M
M
Petrov
S
S
Smirnov
A
A
Alonso
A
A
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005260:Female; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014412:Tumor Virus Infections / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
D004273:DNA, Neoplasm