Vascular system defects and impaired cell chemokinesis as a result of Galpha13 deficiency.
Heterotrimeric GTP-binding proteins (G proteins) participate in cellular signaling and regulate a variety of physiological processes. Disruption of the gene encoding the G protein subunit alpha13 (Galpha13) in mice impaired the ability of endothelial cells to develop into an organized vascular system, resulting in intrauterine death. In addition, Galpha13 (-/-) embryonic fibroblasts showed greatly impaired migratory responses to thrombin. These results demonstrate that Galpha13 participates in the regulation of cell movement in response to specific ligands, as well as in developmental angiogenesis.
Offermanns
S
S
Division of Biology 147-75, California Institute of Technology, Pasadena, CA 91125, USA.
Mancino
V
V
Revel
J P
JP
Simon
M I
MI
D000818:Animals; D001769:Blood; D001920:Bradykinin / Q000494:pharmacology; D002454:Cell Differentiation; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D004730:Endothelium, Vascular / Q000166:cytology* / Q000196:embryology; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D018390:Gene Targeting; D006579:Heterozygote; D006720:Homozygote; D008246:Lysophospholipids / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic; D015398:Signal Transduction; D013917:Thrombin / Q000494:pharmacology
D005353:Fibronectins; D008246:Lysophospholipids; D013917:Thrombin; D019204:GTP-Binding Proteins; D001920:Bradykinin