Cloning and function determination of promoter region of glucoamylase gene from Aspergillus niger T21.
A 850 bp fragment of the 5' flanking region of the glucoamylase gene (glaA) was synthesized from A. niger T21 genome using PCR. The function detection vector was constructed by fusing this fragment to E. coli hygromycin B phosphotransferase gene (hph) and was used to transform A. niger. The high hygromycin resistance transformants thus obtained verified that the synthesized fragment functioned as a promoter in filamentous fungi. Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant.
Tang
G
G
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Tang
W
W
Zhong
L
L
Yang
K
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D002934:Cinnamates; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D005087:Glucan 1,4-alpha-Glucosidase