The role of platelet-derived growth factor in intraluminal stented graft healing.
BACKGROUND
Intraluminally placed polytetrafluoroethylene grafts are associated with enhanced graft endothelialization and diminished intimal hyperplasia when compared with interposition grafts. This study determined the role of platelet-derived growth factor in intraluminal graft healing.


METHODS
Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (control, n = 15) or intraluminal stented (n = 15) grafting. Grafts were explanted at 1, 3, and 6 weeks. The percent of graft area endothelialization and intima to media height ratios were calculated. By using protein electrophoresis and the Western blot technique, platelet-derived growth factor, identified by immunolabeling with anti-platelet-derived growth factor antibody, was isolated from proximal, mid-, and distal graft regions and was quantified using densitometry.


RESULTS
Graft area endothelialization was 0 +/- 3.3 percent, 2.3 +/- 3.3 percent, and 19.0 +/- 3.3 percent for 1-, 3-, and 6-week controls; and 4.7 +/- 3.7 percent, 30.5 +/- 3.3 percent, and 86.8 +/- 3.3 percent for 1-, 3-, and 6-week stented grafts. Endothelialization was greater in stented grafts at 3 and 6 weeks (p < .01). Proximal anastomosis intima to media height ratios were 1.61 +/- 0.15, 1.54 +/- 0.14, and 1.48 +/- 0.15 for 1-, 3-, and 6-week control grafts, and 0.42 +/- 0.18, 0.41 +/- 0.15, and 0.47 +/- 0.14 for 1-, 3-, and 6-week stented grafts. Similar intima to media height ratio values were present at the distal anastomosis. Lower intima to media height ratios were observed in all stented grafts (p < .01). The platelet-derived growth factor content at 1-, 3-, and 6-weeks was lower in all stented grafts when compared with controls. The content of platelet-derived growth factor was greatest in 3-week controls, with a significant difference noted in the mid-graft region (p < .05). The content of platelet-derived growth factor remained stable in all stented graft regions over 6 weeks. An inverse correlation between stented graft platelet-derived growth factor content and endothelialization (r = -0.43) and a positive correlation with proximal anastomotic intimal hyperplasia (r = 0.73) were identified.


CONCLUSIONS
Lower platelet-derived growth factor content is associated with decreased intimal hyperplasia and improved healing in intra-arterial polytetrafluoroethylene grafts.
Ombrellaro
M P
MP
Department of Surgery, University of Tennessee Medical Center, Knoxville 37920, USA.
Stevens
S L
SL
Schaeffer
D O
DO
Freeman
M B
MB
Sciarrotta
J
J
Carroll
R
R
Goldman
M H
MH
D000818:Animals; D001012:Aorta, Abdominal / Q000737:chemistry / Q000502:physiology / Q000601:surgery; D001807:Blood Vessel Prosthesis; D015153:Blotting, Western / Q000379:methods; D004285:Dogs; D004730:Endothelium, Vascular / Q000737:chemistry / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000502:physiology*; D011138:Polytetrafluoroethylene; D015607:Stents; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D011138:Polytetrafluoroethylene