Hypophosphatemia induced in mice by transplantation of a tumor-derived cell line from a patient with oncogenic rickets.
A cultured cell line was derived from a hemangiopericytoma obtained at surgery from a 9 year-old boy with hypophosphatemic rickets. Hypophosphatemia and metabolic bone disease were cured in the patient after tumor removal. Cells from passage 5 were transplanted into 8 week-old athymic female mice. Additional animals received innocula of cells thawed from the stored original tumor tissue, as well as MRC-5 fibroblasts. Serum phosphate levels 3-7 months post-transplantation were lowest in mice which received the cultured cell line (n = 5) 6.1 +/- 0.6 mg/dl (p < 0.05 compared to the other groups), thawed tumor cells (n = 8) 7.2 +/- 0.7 mg/dl, MRC fibroblasts (n = 4) 8.1 +/- 1.0 mg/dl, no transplant (n = 10) 8.7 +/- 1.9 mg/dl. Repeat of the experiment with cultured tumor cells from passage 12 no longer altered phosphate levels. A substance produced and release by the tumor in situ and by tumor-derived cultured cells is capable of producing hypophosphatemia. Experimental manipulation of functional tumor-derived cell lines may help elucidate the factor(s) causing hypophosphatemia in oncogenic osteomalacia/rickets.
Chalew
S A
SA
Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201, USA.
Lovchik
J C
JC
Brown
C M
CM
Sun
C C
CC
D000818:Animals; D002648:Child; D003893:Desmin / Q000032:analysis; D005260:Female; D006393:Hemangiopericytoma / Q000150:complications*; D006801:Humans; D017674:Hypophosphatemia / Q000209:etiology*; D007015:Hypophosphatemia, Familial / Q000209:etiology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D003893:Desmin; D014746:Vimentin