Relationship between DNA synthesis and growth factor expression in primary cultures of adult rat hepatocytes.
In this study we employed primary culture of adult rat hepatocytes to verify the effects of two different extracellular matrices (collagen, matrigel) on EGF-stimulated DNA synthesis and c-myc expression. Our results confirm that in adult rat hepatocytes EGF induces DNA synthesis, preceded by a transient increase of c-myc expression, when cells are cultured at low density on collagen. DNA synthesis appears to be in reciprocal relationship with hepatic expression of IGF-I, IGFBP-1, IGFBP-2 and IGFBP-4, suggesting that IGF-I/IGFBPs system is not involved in liver growth.
Voci
A
A
Istituto di Fisiologia Generale, Università di Genova.
Massajoli
M
M
Bottazzi
C
C
Demori
I
I
Gallo
G
G
D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D011509:Proteoglycans / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C059485:matrigel; D004815:Epidermal Growth Factor; D003094:Collagen; D004247:DNA