[The determination of phosphoethanolamine and other amino components in the lipopolysaccharides of gram-negative bacteria by ion-exchange chromatography].
A method for analysis of phosphoethanolamine in hydrolysates of the fractions structural parts of lipopolysaccharide macromolecule on the column with cation-exchange resin Ostion LG ANB (CSFR) in the system of step gradient of sodium-citrate buffers is suggested, optimal conditions for acidic hydrolysis of polysaccharide-standard were chosen to determine phosphoethanolamine and other amino compounds contained in it. The conditions for chromatography analysis of phosphoethanolamine together with aminosugars and amino acids have been developed. Using the suggested procedure the presence of phosphoethanolamine was found in preparations of lipid A hydrolysates and core oligosaccharide isolated from some lipopolysaccharide strains of Pseudomonas fluorescens.
Veremeĭchenko
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D000596:Amino Acids / Q000032:analysis*; D000606:Amino Sugars / Q000032:analysis*; D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods; D004983:Ethanolamines / Q000032:analysis*; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis*; D013997:Time Factors
D000596:Amino Acids; D000606:Amino Sugars; D002021:Buffers; D004983:Ethanolamines; D008070:Lipopolysaccharides; C005448:phosphorylethanolamine