Cytomegalovirus glycoprotein B-specific antibody analysis using electrochemiluminescence detection-based techniques.
An electrochemiluminescence technique was used to develop versatile and sensitive assay strategies for determination of seroreactivities against biologically important cytomegalovirus neutralization epitopes expressed on glycoprotein B. Indirect binding assays showed wide linear assay ranges and revealed that serum samples diluted in parallel with a monoclonal antibody-based standard, simplifying quantitative analytical assessments.
Ohlin
M
M
Department of Immunotechnology, Lund University, Sweden. mats.ohlin@immun.lth.se
Silvestri
M
M
Sundqvist
V A
VA
Borrebaeck
C A
CA
D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology*; D007159:Immunologic Tests; D008163:Luminescent Measurements; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus