[Inhibiting effects of mixtures of alpha-tocopherol with beta-carotene or vitamin A during oxidation of polyunsaturated fatty acid esters].
The inhibitory activities of individual natural antioxdants (AOs), such as alpha-tocopherol (I), beta-carotene (II), vitamin A(III) and their mixtures were comparatively studied when oxidation of microbial polyunsaturated fatty acid esters that predominately contain ethyl arachidonate was initiated; effects of antagonism were found in the combined action of I and II, I and III. The magnitude of antagonism effect was estimated from the difference between the total additive effects of individual AOs and the experimentally determined effects of their mixture. There is a U-dependence of changes in the antagonism values upon II concentrations at steady-state I levels. It was found that in the range of low (10(-8) to 10(-7) M) and high (10(-2) M) concentrations of II, the involvement of antagonism might be relatively low (3.5-4.0%); in the range of 10(-6) to 10(-5) M, there is a concentration "hole" whose peak (1.25 x 10(-6) M) is as high as 90%. As I levels elevated, antagonism in the mixture decreased. It is suggested that the intermolecular interactions of natural AO during oxidation represent a mechanism for the body's antioxidative homeostasis.
Storozhok
N M
NM
Kutuzova
I V
IV
D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D004952:Esters; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D014801:Vitamin A / Q000008:administration & dosage / Q000494:pharmacology*; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D019207:beta Carotene / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D004952:Esters; D005231:Fatty Acids, Unsaturated; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E