A new screening method for the selection of Lactobacillus acidophilus group lactic acid bacteria with high adhesion to human colonic mucosa.
A hemagglutination (HA) assay was done for the screening of lectin-like components in surface layer protein (SLP) from Lactobacillus (L.) acidophilus A group strains. The new screening method, using polystyrene beads coated with rat-colonic mucin (RCM), which combines sugar chains similar to those of human colonic mucin, was also done. The results showed that the HA assay was not a good indicator for selecting strains having high adhesion to the human intestinal tract. The SLPs from 3 strains that strongly bound to RCM also bound well to carbohydrate portions of Carnoy's-fixed human colonic mucous layer. These results suggest that this method is a new promising screening technique for the L. acidophilus strains having high adhesion to the human intestinal tract.
Takahashi
N
N
Laboratory of Animal Products Chemistry, Faculty of Agriculture, Tohoku University, Japan.
Saito
T
T
Ohwada
S
S
Ota
H
H
Hashiba
H
H
Itoh
T
T
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002241:Carbohydrates / Q000032:analysis; D003106:Colon / Q000382:microbiology; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006651:Histocytochemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism*; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D037102:Lectins / Q000032:analysis*; D008297:Male; D009077:Mucins / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D002241:Carbohydrates; D037102:Lectins; D009077:Mucins; D019344:Lactic Acid