title: New HLA-DR haplotypes containing the DRB6 pseudogene.
abstract: An unexpected probe reaction pattern was observed in two samples during HLA-DR typing by PCR-Sequence Specific Oligonucleotide Probes. In order to confirm the unusual typings, samples were analyzed by PCR-Sequence Specific Primers, cloning, and nucleotide sequencing of the second exon of the HLA-DRB-genes. The confirmed DR, DQ phenotype for one sample was DRB1*0701, DRB4*01, DRB5*0101, DRB6*0201, DQB*0602, DQB1*0202. The phenotype of other sample was DRB1*1602, DRB1*1302, DRB3*0301, DRB6*0101, DQB1*0501, DQB1*0502. The first sample has the novel combination of DRB1*0701 with DRB5*0101 and DRB6*0201. The second sample has either DRB6*0101 together with DRB1*1602 in absence of any DRB5 allele or DRB6*0101 together with DRB1*1302, DRB3*0301. We postulate that the most likely haplotype in sample #1 is DRB1*0701; DRB5*0101, DRB1*0602 which could have arisen from gene conversion. The most likely haplotype in sample #2, DRB1*1602, DRB6*0101, DQB1*0502 would have arisen from an homologous recombination event.
authors.affiliation: American Red Cross, New England Region, Dedham, Massachusetts, USA.
authors.forename: M
authors.initials: M
authors.lastname: Salazar
authors.forename: C B
authors.initials: CB
authors.lastname: Granja
authors.forename: A
authors.initials: A
authors.lastname: Selvakumar
authors.forename: B
authors.initials: B
authors.lastname: Fresia
authors.forename: S
authors.initials: S
authors.lastname: Alosco
authors.forename: B
authors.initials: B
authors.lastname: Dupont
authors.forename: E J
authors.initials: EJ
authors.lastname: Yunis
mesh_terms: D006684:HLA-DR Antigens / Q000235:genetics*; D059806:HLA-DR beta-Chains; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction; D011544:Pseudogenes
chemical_list: D006684:HLA-DR Antigens; D059806:HLA-DR beta-Chains; C069699:HLA-DRB6 antigen