Comparison of growth factor receptors and metabolic characteristics of satellite cells derived from the biceps femoris and pectoralis major muscles of the turkey.
Myogenic satellite cells were isolated from the turkey pectoralis major (PM), a muscle composed largely of white fibers, and the biceps femoris (BF), a muscle composed largely of red fibers, and their properties were compared in culture. Satellite cells derived from the PM and BF muscles exhibited differences in metabolic parameters, growth factor receptor characteristics, and mitogenic responses. PM satellite cells exhibited greater responsiveness to platelet-derived growth factor (PDGF), and the PDGF receptor on these cells had a higher affinity toward ligand compared to BF cells (P < 0.05). Protein synthesis, protein degradation, and glucose uptake rates were higher in BF satellite cell cultures (P < 0.05), correlating with previously reported in vivo measurements using red and white muscle fibers.
McFarland
D C
DC
Department of Animal and Range Sciences, South Dakota State University, Brookings, South Dakota, 57007-0392, USA.
Gilkerson
K K
KK
Pesall
J E
JE
Wellenreiter
R H
RH
Ferrin
N H
NH
Ye
W V
WV
Yun
Y
Y
Vander Wal
L S
LS
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D010369:Pectoralis Muscles / Q000166:cytology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D014422:Turkeys
D006133:Growth Substances; D007457:Iodine Radioisotopes; D010982:Platelet-Derived Growth Factor; D017468:Receptors, Fibroblast Growth Factor; D017978:Receptors, Growth Factor; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1; D017479:Receptors, Platelet-Derived Growth Factor