title: Competitive and indirect enzyme-linked immunosorbent assays for Mycobacterium bovis infections based on MPB70 and lipoarabinomannan antigens.
abstract: A competitive enzyme-linked immunosorbent assay (C-ELISA) using M. bovis BCG Tokyo culture filtrate as antigen and anti-MPB70 4C3/17 monoclonal antibody was developed for use in multiple animal species. An analysis of the C-ELISA data for cattle and bison serum panels revealed specificities of 68% to 85% and sensitivities of 85% to 89%. Receiver operator characteristics (ROC) of this data revealed areas of 81% to 92% for C-ELISA and demonstrated that C-ELISA as well as the indirect ELISA protocols, MPB70-ELISA and LAM-ELISA, discriminate M. bovis infected animals from non-infected animals for these particular panels. The kappa statistic values for agreement beyond chance between C-ELISA and MPB70-ELISA were determined after ELISA cutoffs were adjusted to minimize false positives. There were poor to excellent agreements between C-ELISA and MPB70-ELISA in all species tested (Bovidae, Cervidae, and Camelidae) that were consistently higher than the kappa statistic between C-ELISA and LAM-ELISA. The humoral response to one antigen and little or no response to the other in many animals argued for a parallel interpretation of C-ELISA and LAM-ELISA to increase sensitivity.
authors.affiliation: Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario.
authors.forename: E A
authors.initials: EA
authors.lastname: Sugden
authors.forename: K
authors.initials: K
authors.lastname: Stilwell
authors.forename: E B
authors.initials: EB
authors.lastname: Rohonczy
authors.forename: P
authors.initials: P
authors.lastname: Martineau
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D016164:Bison; D002161:Camelids, New World; D002417:Cattle; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008070:Lipopolysaccharides / Q000276:immunology*; D009163:Mycobacterium bovis; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; C060216:MPB70 protein, Mycobacterium bovis