Deficiencies of myocardial troponin-T and creatine kinase MB isoenzyme in dogs with idiopathic dilated cardiomyopathy.
OBJECTIVE
To test the hypothesis that the derangement of myocardial energy homeostasis characteristic of idiopathic dilated cardiomyopathy (IDCM) of Doberman Pinschers was attributable to deficiency of creatine kinase (CK) isoenzyme MB relative to CK isoenzyme MM and troponin-T (Tn-T).


METHODS
9 Doberman Pinschers with advanced congestive heart failure attributable to IDCM and 9 mixed-breed dogs without cardiac disease (controls).


METHODS
Myocardial myofibrillar CK-MM, mitochondrial CK, and cytosolic CK-MB activities were determined in comparison with the concentration of cardiac-specific, myofibrillar protein Tn-T. Myocardial biopsy specimens were obtained after euthanasia and were ultrafrozen. Isoenzymes of CK were separated electrophoretically, and their activity was determined, using a coupled-enzyme, kinetic, fluorescent assay and densitometry. Troponin-T content was determined by immunoassay.


RESULTS
Myofibrillar CK-MM and mitochondrial CK activities and Tn-T content were 25% lower in the dogs with IDCM than in controls, whereas cytosolic CK-MB activity was 50% lower.


CONCLUSIONS
Deficiency of CK-MB activity is a component of and may have a central role in the energy deficiency characteristic of failing myocardium in IDCM of Doberman Pinschers. A side-product of this study was the observation that, in dogs, CK-MB activity and Tn-T content of myocardium are sufficiently high to be candidate serum markers for active cardiac injury.
O'Brien
P J
PJ
Human Safety Department 500 Miami Valley Laboratories, Procter & Gamble, Cincinnati, OH 45253-8707, USA.
D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary; D002311:Cardiomyopathy, Dilated / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D003402:Creatine Kinase / Q000032:analysis* / Q000172:deficiency; D003600:Cytosol / Q000201:enzymology; D003720:Densitometry / Q000662:veterinary; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D004285:Dogs; D004734:Energy Metabolism / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005453:Fluorescence; D007527:Isoenzymes; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D010084:Oxidation-Reduction; D014336:Troponin / Q000032:analysis* / Q000172:deficiency; D020107:Troponin T
D007527:Isoenzymes; D014336:Troponin; D020107:Troponin T; D003402:Creatine Kinase