A sialic acid-binding lectin from ovine placenta: purification, specificity and interaction with actin.
A sialic-acid-specific lectin from ovine placental cotyledons was purified by affinity chromatography on bovine submaxillary mucin-agarose followed by gel filtration, and it showed a molecular weight of 65000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. This lectin has the capacity to interact with actin, since it binds to actin-F in a cosedimentation assay and it acts as a mediator in the binding of actin to the affinity column. The lectin agglutinated rabbit and rat erythrocytes, but not human A, B or O erythrocytes. Haemagglutination inhibition assays of different saccharides, glycoproteins and glycolipids indicate that this lectin has affinity for sialic acid, which is enhanced by its O-acetylation. The N-terminal sequence of the protein shows 92% identity with rabbit and porcine uterine calreticulin.
Iglesias
M M
MM
Instituto de Química y Fisicoquimica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Cymes
G D
GD
Wolfenstein-Todel
C
C
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry; D037282:Calreticulin; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005732:Gangliosides / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009077:Mucins / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid / Q000378:metabolism* / Q000494:pharmacology; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D012261:Ribonucleoproteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013379:Substrate Specificity
D000199:Actins; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D005732:Gangliosides; D037102:Lectins; D009077:Mucins; D012261:Ribonucleoproteins; D019158:N-Acetylneuraminic Acid; D005947:Glucose