Biochemical characterization of recombinant fusions of lipopolysaccharide binding protein and bactericidal/permeability-increasing protein. Implications in biological activity.
The physiological response to endotoxin (lipopolysaccharide (LPS)) can be regulated by two closely related LPS-binding proteins, LPS-binding protein (LBP), which potentiates LPS' inflammatory activity via interaction with the monocytic antigen CD14, and bactericidal/permeability-increasing protein (BPI), which neutralizes LPS. Both proteins bind LPS with high affinity sites in their N-terminal domains, whereas interaction between LBP and CD14 is dependent upon the LBP C-terminal domain. We have created fusions of the N- and C-terminal domains from each protein and compared the functional activities and pharmacokinetics of these fusions, the individual N-terminal domains, and the parent proteins. The N-terminal domains of BPI and LBP bound lipid A with their characteristic apparent affinity constants, regardless of the C-terminal fusion partner. In addition, the C-terminal domain of LBP allowed transfer of LPS to CD14 in conjunction with either N-terminal LPS binding domain. Proteins containing a BPI N-terminal domain had greater heparin binding capacities in vitro and were cleared more rapidly from the plasma of whole animals. Taken together, these data better define how closely related proteins such as BPI and LBP can have opposing effects on the body's response to LPS.
Abrahamson
S L
SL
XOMA Corporation, Berkeley, California 94710 and the XOMA Corporation, Santa Monica, California 90404, USA.
Wu
H M
HM
Williams
R E
RE
Der
K
K
Ottah
N
N
Little
R
R
Gazzano-Santoro
H
H
Theofan
G
G
Bauer
R
R
Leigh
S
S
Orme
A
A
Horwitz
A H
AH
Carroll
S F
SF
Dedrick
R L
RL
D000209:Acute-Phase Proteins / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides; D001667:Binding, Competitive; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D006493:Heparin / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010957:Plasmids; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D006493:Heparin; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein