Synthetic vaccine against foot-and-mouth disease based on a palmitoyl derivative of the VP1 protein 135-159 fragment of the A22 virus strain.
The peptide Palm2 135-159, a dipalmitoyl derivative of the 135-159 fragment of VP1 protein of the foot-and-mouth disease virus strain A22 was synthesized. In the experiments on mice, guinea pigs and sheep Palm2 135-159 possesses greater immunogenic and protective activity than the nonacylated 135-159 peptide. The synthetic vaccine against foot-and-mouth disease for use in sheep was developed on the basis of the lipopeptide. Synthetic polymethylsiloxane oil was found to be a suitable adjuvant for this vaccine. The dependencies of protective and immunogenic effects from the dose of peptide were studied. The vaccine was found to be stable to storage for 1 year at 18 degrees C. It was shown that the synthetic vaccine provides 1 year protection of sheep against foot-and-mouth disease after a single administration. The vaccine is allowed for veterinary use in Russia.
Volpina
O M
OM
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, Moscow, Russian Federation.
Yarov
A V
AV
Zhmak
M N
MN
Kuprianova
M A
MA
Chepurkin
A V
AV
Toloknov
A S
AS
Ivanov
V T
VT
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D006168:Guinea Pigs; D051379:Mice; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D012756:Sheep; D014614:Vaccines, Synthetic / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D010446:Peptide Fragments; D014614:Vaccines, Synthetic