title: Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato.
abstract: The plant pathogen Botrytis cinerea can infect undamaged plant tissue directly by penetration of the cuticle. This penetration has been suggested to be enzyme-mediated, and an important role for cutinase in the infection process has been proposed. In this study the expression of the cutinase encoding gene cutA of B. cinerea was analyzed using a cutA promoter-GUS reporter gene fusion. Transformants containing the fusion construct were examined for GUS expression on gerbera flowers and tomato fruits. High GUS activity was detected from the onset of conidial germination and during penetration into epidermal cells, indicating that cutA is expressed during the early stages of infection. To determine the biological relevance of cutinase A for successful penetration, cutinase A-deficient mutants were constructed by gene disruption. Pathogenicity of two transformants lacking a functional cutA gene was studied on gerbera flowers and tomato fruits. Their ability to penetrate and cause symptoms was unaltered compared to the wild-type strain. These results exclude an important role for cutinase A during direct penetration of host tissue by B. cinerea.
authors.affiliation: Department of Phytopathology, Wageningen Agricultural University, The Netherlands. jan.vankan@medew.fyto.wau.nl
authors.forename: J A
authors.initials: JA
authors.lastname: van Kan
authors.forename: J W
authors.initials: JW
authors.lastname: van't Klooster
authors.forename: C A
authors.initials: CA
authors.lastname: Wagemakers
authors.forename: D C
authors.initials: DC
authors.lastname: Dees
authors.forename: C J
authors.initials: CJ
authors.lastname: van der Vlugt-Bergmans
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005966:Glucuronidase / Q000096:biosynthesis; D018551:Solanum lycopersicum / Q000382:microbiology*; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D011993:Recombinant Fusion Proteins; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D005966:Glucuronidase