Analyses of traA, int-Tn, and xis-Tn mutations in the conjugative transposon Tn916 in Enterococcus faecalis.
The conjugative transposon Tn916 moves intercellularly via an excision/insertion mechanism that involves products of int-Tn and xis-Tn. Tn5-insertion mutations in these genes were found to be complemented in an Enterococcus faecalis host by specific coresident transposons harboring the corresponding wild-type allele. A determinant designated traA, partially overlapping and divergently transcribed from xis-Tn, is thought to encode a key positively acting regulatory protein needed for expression of conjugation functions. This locus was also shown to express a trans-acting product.
Jaworski
D D
DD
Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Flannagan
S E
SE
Clewell
D B
DB
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D013293:Enterococcus faecalis / Q000235:genetics*; D036781:Fimbriae Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional
D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D036781:Fimbriae Proteins