Influence of hypoxia and hypoxia-reoxygenation on endothelial P-selectin expression.
P-selectin is an endothelial adhesion molecule involved in the initial step of the neutrophil recruitment. We investigated the effect of hypoxia (95% N2, 5% CO2) and of hypoxia-reoxygenation (95% air, 5% CO2) on the expression of P-selectin by human umbilical vein endothelial cells (HUVEC). P-selectin expression was detected by immunolabelling and quantified by flow cytometric analysis. Our data indicate that hypoxia induces an increase in P-selectin expression with a maximum reached after 90 minutes. A hypoxic exposure of 90 minutes results in a highly significant increase compared to normoxia (p < 0.001, n = 13). Furthermore, when a reoxygenation period follows 90 minutes of hypoxia, the initially elevated levels of P-selectin are dramatically enhanced with a maximum obtained after 60 minutes of reoxygenation.
Closse
C
C
Laboratories of Hematology, University of Bordeaux II, Pessac, France.
Seigneur
M
M
Renard
M
M
Pruvost
A
A
Dumain
P
P
Belloc
F
F
Boisseau
M R
MR
D015687:Cell Hypoxia / Q000235:genetics*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007511:Ischemia / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D019007:P-Selectin / Q000096:biosynthesis* / Q000235:genetics; D015424:Reperfusion; D014471:Umbilical Veins
D019007:P-Selectin; D010100:Oxygen