NMDA channel regulation by channel-associated protein tyrosine kinase Src.
The N-methyl-D-aspartate (NMDA) receptor mediates synaptic transmission and plasticity in the central nervous system (CNS) and is regulated by tyrosine phosphorylation. In membrane patches excised from mammalian central neurons, the endogenous tyrosine kinase Src was shown to regulate the activity of NMDA channels. The action of Src required a sequence [Src(40-58)] within the noncatalytic, unique domain of Src. In addition, Src coprecipitated with NMDA receptor proteins. Finally, endogenous Src regulated the function of NMDA receptors at synapses. Thus, NMDA receptor regulation by Src may be important in development, plasticity, and pathology in the CNS.
Yu
X M
XM
Division of Neuroscience, Hospital for Sick Children, Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada.
Askalan
R
R
Keil
G J
GJ
Salter
M W
MW
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism*; D008969:Molecular Sequence Data; D016202:N-Methylaspartate / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission; D019061:src-Family Kinases / Q000737:chemistry / Q000378:metabolism*
D007473:Ion Channels; D009842:Oligopeptides; D016194:Receptors, N-Methyl-D-Aspartate; D019000:Phosphotyrosine; D016202:N-Methylaspartate; D019061:src-Family Kinases