Mutagenic response to benzene and tris(2,3-dibromopropyl)-phosphate in the lambda lacI transgenic mouse mutation assay: a standardized approach to in vivo mutation analysis.
The genotoxic response of benzene and tris(2,3-dibromopropyl)-phosphate (TDBP) have been evaluated in several tissues using the standardized lambda/lacI (Big Blue) transgenic mouse mutation assay. Separate groups of four to five male B6C3F1 transgenic lambda/lacI mice were given oral administrations of benzene or TDBP at varying concentrations. Tissues evaluated include lung, bone marrow, and spleen in benzene-treated animals, and liver, kidney, and stomach in TDBP-treated animals. Significant increases in lacI mutations were observed in the spleen and bone marrow of benzene treated mice, and the kidneys of TDBP-treated mice. Where applicable, mutagenesis patterns of tissue sensitivity were consistent with what has been observed previously in other assays. In addition, mutagenicity in tissues not traditionally evaluated for mutations correlated to sites of carcinogenicity for the chemicals tested.
Provost
G S
GS
Stratagene, La Jolla, CA 92037, USA.
Mirsalis
J C
JC
Rogers
B J
BJ
Short
J M
JM
D000818:Animals; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics*; D001554:Benzene / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D029968:Escherichia coli Proteins; D005260:Female; D056968:Lac Repressors; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000235:genetics*; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010755:Organophosphates / Q000633:toxicity*; D012097:Repressor Proteins / Q000187:drug effects / Q000235:genetics*; D014018:Tissue Distribution
D001426:Bacterial Proteins; D002273:Carcinogens; D029968:Escherichia coli Proteins; D056968:Lac Repressors; D009153:Mutagens; D010755:Organophosphates; D012097:Repressor Proteins; C012085:tris(2,3-dibromopropyl)phosphate; D001554:Benzene