Cloning of stress tolerance gene in Torulaspora delbrueckii No. 3110.
A cryptic plasmid (pSRY1) was found in Torulaspora delbrueckii No. 3110. A plasmid vector (pSRY21) was constructed by ligating it to pPR1-RIM-C ORF with a cycloheximide resistance gene (RIM-C). The transformation efficiency of the strain No. 3110 with pSRY21 by the protoplast-PEG method was increased by 3000-fold by treatment of the protoplasts with polylysine [poly(epsilon-L-lysine)]. Previously we have reported a mutant No. 3110, T1, which can neither assimilate nor accumulate trehalose. The mutant was salt- and temperature-sensitive. We cloned a DNA fragment from No. 3110, which, when introduced, complemented these mutant characters of T1. These results suggested an important role of metabolism of trehalose for the stress tolerance.
Nakata
K
K
Central Research Laboratories, Mercian Corporation, Kanagawa, Japan.
Okamura
K
K
D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D005822:Genetic Vectors; D006358:Hot Temperature; D009154:Mutation; D010957:Plasmids; D011107:Polylysine / Q000378:metabolism*; D004718:Saccharomycetales / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic; D014199:Trehalose / Q000235:genetics
D004268:DNA-Binding Proteins; D011107:Polylysine; D012965:Sodium Chloride; D014199:Trehalose