Structural organization of the pre-mRNA splicing commitment: a hypothesis.
Several models have been presented in the past to explain localized distributions of nuclear RNAs from individual genes that range from small foci to more elongated "track-like" structures. We present here a hypothesis which explains that, in the case of regulated splicing, there is in diploid cells a spatial separation of transcription sites from the execution of regulated splicing which we situate to domains of SR protein accumulation. In addition, it explains the presence of poly(A) sequences, and the lack of the autoradiographic label due to short pulses of [3H]uridine, in these domains.
Melcák
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Department of Cell Biology, Academy of Sciences of Czech Republic, Prague, Czech Republic.
Raska
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I
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D008954:Models, Biological; D015394:Molecular Structure; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing
D012322:RNA Precursors