title: The cloning, sequence analysis, and expression in E. coli of coat protein gene of grapevine fanleaf virus Gh.
abstract: Fragments of 5' end and 3' end of the coat protein gene of a Chinese strain grapevine fanleaf virus (GFLV-Gh) were separately amplified through RT-PCR. The two products were cloned and ligated into a complete full-length coat protein gene via an inherent BgIII restriction site. The entire gene contains 1512 nucleotides which encode a 504 amino acids coat protein, molecular weight approximately 56 kDa. Comparison with GFLV F-13 shows 88.4% homology in nucleotides and 95.8% similarity in deduced amino acid residues. Also this coat protein gene was expressed in E. coli DH 5 alpha.
authors.affiliation: Department of Virology, Chinese Academy of Sciences, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Guan
authors.forename: W
authors.initials: W
authors.lastname: Cai
authors.forename: K
authors.initials: K
authors.lastname: Mang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005638:Fruit / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D011994:Recombinant Proteins