title: Isolation and promoter mapping of the gene encoding murine co-stimulatory factor B7-1.
abstract: B7-1 is one of the co-stimulatory factors which plays an important role in immunity. We have cloned and functionally mapped the promoter of the murine B7-1 gene using transient transfection assays in mouse L (tk-) cells. The B7-1 basal promoter consists of three positively regulated regions. The distal region, located at -2597 to -1555, contains an assortment of putative transcription factor binding sites. The proximal upstream region, located at -130 to -110, contains a tandem repeat sequence 5'-GTGTTCTAGTGTT-3'. A downstream region is positioned at +269 to +25. We have also identified an alternatively spliced form of the murine B7-1 gene in L (tk-) cells.
authors.affiliation: Department of Growth Factor Research, Abbott Laboratories, Abbott Park, IL 60064, USA. zhangh@randb.abbott.com
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Haasch
authors.forename: K B
authors.initials: KB
authors.lastname: Idler
authors.forename: G F
authors.initials: GF
authors.lastname: Okasinski
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D005796:Genes / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D007739:L Cells; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
chemical_list: D018122:B7-1 Antigen; D012333:RNA, Messenger; D014157:Transcription Factors; D008156:Luciferases