Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli.
An expression plasmid carrying an alkaline lipase gene from Proteus vulgaris was constructed. The lipase content in Escherichia coli cells harboring the expression plasmid reached about 22% of total soluble protein. The purified enzyme displayed a partial interfacial activation toward p-nitrophenyl butyrate (PNPB).
Kim
H K
HK
Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Yusung, Taejon, South Korea.
Park
Y S
YS
Kim
H
H
Oh
T K
TK
D002087:Butyrates / Q000819:agonists*; D004926:Escherichia coli; D015870:Gene Expression; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011514:Proteus vulgaris; D011994:Recombinant Proteins / Q000096:biosynthesis
D002087:Butyrates; D011994:Recombinant Proteins; C033592:4-nitrophenyl butyrate; D008049:Lipase