Cell-based screen for identification of inhibitors of tubulin polymerization.
This assay is based on morphological changes of rat glioma cells treated with db-cAMP. The db-cAMP treatment induces a tubulin-dependent change causing the cells to acquire a spherical shape. Pretreatment with tubulin inhibitors brings about the disintegration of tubulin polymer and/or prevents its polymerization. Cells with inhibited tubulin fail to respond to db-cAMP treatment. Cells treated with inhibitors of tubulin polymerization are then separated from the spherical cells by aspiration. A semiautomated scanning procedure evaluates the final culture density and yields graphical data.
Kokoshka
J M
JM
University of Utah, Department of Pharmacology and Toxicology, Salt Lake City 84112, USA.
Ireland
C M
CM
Barrows
L R
LR
D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology; D003994:Bucladesine; D002454:Cell Differentiation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005562:Formazans / Q000494:pharmacology; D005910:Glioma / Q000188:drug therapy / Q000473:pathology; D051381:Rats; D014404:Tubulin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005562:Formazans; D014404:Tubulin; D003994:Bucladesine