Novel anti-calcification treatment of biological tissues by grafting of sulphonated poly(ethylene oxide).
Biological porcine tissue was modified by the direct coupling of sulphonated poly(ethylene oxide) (PEO-SO3) containing amino acid end groups after glutaraldehyde fixation. The calcification of the modified tissue [bioprosthetic tissue (BT)-PEO-SO3] and control (BT control) was investigated by in vivo rate subdermal, canine aorta-illiac shunt and right ventricle-pulmonary artery shunt implantation models. Less calcium deposition of BT-PEO-SP3 than of BT control was observed in in vivo tests. Such a reduced calcification of BT-PEO-SO3 can be explained by decreases of residual glutaraldehyde groups, a space filling effect and, therefore, improved biostability and synergistic blood-compatible effects of PEO and SO3 groups after the covalent binding of PEO-SO3 to tissue. This simple method can be a useful anti-calcification treatment for implantable tissue valves.
Park
K D
KD
Polymer Chemistry Laboratory, Korea Institute of Science and Technology, Seoul, Korea.
Lee
W K
WK
Yun
J Y
JY
Han
D K
DK
Kim
S H
SH
Kim
Y H
YH
Kim
H M
HM
Kim
K T
KT
D000818:Animals; D001021:Aortic Valve / Q000378:metabolism*; D001166:Arteriovenous Shunt, Surgical; D001807:Blood Vessel Prosthesis; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D004285:Dogs; D006350:Heart Valve Prosthesis; D008297:Male; D011092:Polyethylene Glycols; D019736:Prostheses and Implants; D051381:Rats; D013451:Sulfonic Acids; D013552:Swine
D013451:Sulfonic Acids; D011092:Polyethylene Glycols; D002118:Calcium