The amino acid substitutions Ser288Gly and Val320Ala convert the cortisol producing enzyme, CYP11B1, into an aldosterone producing enzyme.
Transfection studies with cDNAs encoding hybrids between the highly similar cytochrome P450 enzymes, CYP11B1 (steroid 11 beta-hydroxylase) and CYP11B2 (aldosterone synthase) have identified which amino acids determine the different activities of the enzymes.
Curnow
K M
KM
Mulatero
P
P
Emeric-Blanchouin
N
N
Aupetit-Faisant
B
B
Corvol
P
P
Pascoe
L
L
D000450:Aldosterone / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics*; D018076:DNA, Complementary; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis*; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013252:Steroid 11-beta-Hydroxylase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D000596:Amino Acids; D018076:DNA, Complementary; D000450:Aldosterone; D013252:Steroid 11-beta-Hydroxylase; D006854:Hydrocortisone