title: Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter.
abstract: To understand the regulation of the BMP-2 gene expression, we recently isolated the BMP-2 gene from a mouse genomic library and characterized the exon-intron structure and promoter. RNase protection assay using poly (A)+ RNA of mouse osteoblasts demonstrates that two regions in BMP-2 gene are protected by antisense mouse BMP-2 RNA probes. These results demonstrate that BMP-2 gene utilizes two alternative promoters, a distal and a proximal promoter. In the present study we demonstrate that BMP-2 mRNA from rapidly growing deer antler tissue has an extended 5' non-coding region compared with the human and rat BMP-2 mRNA. The extended 5' non-coding region in the deer mRNA represents transcripts from the upstream distal promoter. This is the first evidence of a natural BMP-2 mRNA from a bone-forming tissue that most likely initiated from the distal transcription start site.
authors.affiliation: Department of Medicine, University of Texas Health Science Center at San Antonio 78284, USA.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Feng
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: N
authors.initials: N
authors.lastname: Ghosh-Choudhury
authors.forename: J
authors.initials: J
authors.lastname: Esparza
authors.forename: G R
authors.initials: GR
authors.lastname: Mundy
authors.forename: S E
authors.initials: SE
authors.lastname: Harris
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000999:Antlers / Q000254:growth & development / Q000378:metabolism*; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics*; D003670:Deer; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D015698:Genomic Library; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015347:RNA Probes; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D015347:RNA Probes; D016212:Transforming Growth Factor beta