Differential localization of three distinct binding sites for sigma receptor ligands in rat spleen.
In vitro receptor autoradiography was used to localize sigma 1 receptors, sigma 2 receptors, and novel haloperidol/DTG-inaccessible sites for sigma and opiate ligands in rat spleen. Sigma-1 receptors were present throughout the spleen, but were most concentrated in the T cell zones. Binding under "sigma 2 receptor-selective' conditions was 70% nonspecific, and sigma 2 receptors could not be detected. Haloperidol/DTG-inaccessible sites had a coarse, punctate distribution in the red pulp and marginal zones of the white pulp. This anatomical localization suggests types of cells and functions that should be examined for modulation by sigma receptors.
Wolfe
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Department of Medical Microbiology and Immunology, Ohio State University College of Medicine, Columbus 43210, USA. wolfe.22@osu.edu
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Whitlock
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Saini
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D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D014150:Antipsychotic Agents / Q000494:pharmacology; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D005260:Female; D006146:Guanidines / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008024:Ligands; D008297:Male; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010423:Pentazocine / Q000494:pharmacology; D010620:Phenazocine / Q000031:analogs & derivatives / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017480:Receptors, sigma / Q000737:chemistry / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry* / Q000378:metabolism; D014316:Tritium
D000701:Analgesics, Opioid; D000927:Anticonvulsants; D014150:Antipsychotic Agents; D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D006146:Guanidines; D008024:Ligands; D009292:Narcotic Antagonists; D010880:Piperidines; D017480:Receptors, sigma; D014316:Tritium; D009271:Naltrexone; C012134:SK&F 10047; C026539:preclamol; D010620:Phenazocine; D006220:Haloperidol; C050232:1,3-ditolylguanidine; D010423:Pentazocine