Kainic acid blocks a TTX-sensitive sodium channel in retinal horizontal cells of the turtle (Pseudemys scripta elegans).
The aim of this study was to investigate the effects of excitatory amino acids on channels found in horizontal cell membranes using patch-clamp techniques. We unexpectedly found that the excitatory amino acid receptor agonist, kainic acid, reversibly inhibited the transient tetrodotoxin (TTX)-sensitive Na+ current in isolated horizontal cell bodies and axons from the retina of the turtle (Pseudemys scripta elegans). The effect of kainic acid was antagonized by the glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione. Kainic acid activated a non-selective cation current, a finding that was consistent with previous reports, and which would account for the kainate induced depolarisation of these cells. The inhibition of the transient TTX-sensitive Na+ current by kainic acid might be important in the modification of the kinetics of responses to excitatory amino acid analogues often observed during intracellular recording from these cells.
Martin
D K
DK
Department of Clinical Pharmacology, St. Vincent's Hospital, Darlinghurst, NSW, Australia.
Anderton
P J
PJ
Bursill
J A
JA
Le Dain
A C
AC
Millar
T J
TJ
D000818:Animals; D007608:Kainic Acid / Q000494:pharmacology*; D012160:Retina / Q000187:drug effects*; D015222:Sodium Channels / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology*; D014426:Turtles
D015222:Sodium Channels; D013779:Tetrodotoxin; D007608:Kainic Acid