Deletion within the D17S34 locus in a primitive neuroectodermal tumor.
Loss of heterozygosity on chromosome 17p13.3 is frequently observed in solid tumors, and the presence of a tumor suppressor gene has been predicted in this region of chromosome 17. We have analyzed a primitive neuroectodermal tumor sample exhibiting loss of heterozygosity at the D17S34 locus, a commonly used telomeric marker on the short arm of chromosome 17. The remaining allele showed a rearrangement. Cosmids spanning the D17S34 locus and probes from that region were used to demonstrate a 9-kb deletion within the D17S34 locus and were found to contain evolutionary, conserved sequences. Genetic alterations in this region may also affect expression of immediately adjacent genes, such as ABR, and could be a common mechanism in the causation of primitive neuroectodermal tumors.
Haataja
L
L
Department of Pathology, Children's Hospital of Los Angeles Research Institute, California 90027, USA.
Raffel
C
C
Ledbetter
D H
DH
Tanigami
A
A
Petersen
D
D
Heisterkamp
N
N
Groffen
J
J
D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D017353:Gene Deletion; D015321:Gene Rearrangement; D005819:Genetic Markers; D005822:Genetic Vectors; D006801:Humans; D018242:Neuroectodermal Tumors, Primitive / Q000235:genetics*
D005819:Genetic Markers