Reduced anti-Pseudomonas aeruginosa activity of a cephalosporin, cefodizime, in rats whose neutrophils were selectively depleted by a monoclonal antibody.
In order to clarify the mechanisms of the in vivo antibacterial activity of a cephalosporin, cefodizime (CDZM), the effect of this antibiotic on Pseudomonas aeruginosa E7 infection was examined in rats whose neutrophils had been selectively depleted by monoclonal antibody RP-3. CDZM was less effective in RP-3-treated rats than in untreated rats. However, treatment of rats with recombinant human granulocyte-colony stimulating factor (rhG-CSF) augmented the in vivo activity of this antibiotic. Furthermore, the in vivo antibacterial activity of two other cephalosporins, cefpimizole (CPIZ) and cefoperazone (CPZ), was bilaterally affected by a rise or fall in the neutrophil number, although to a lesser degree than was the case with CDZM. Taken together, neutrophils play an important role in the in vivo antibacterial activity of certain cephalosporins.
Araki
A
A
Department of Immunology and Parasitology, Yamagata University School of Medicine, Japan.
Sendo
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000276:immunology; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D007958:Leukocyte Count / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D002511:Cephalosporins; D016179:Granulocyte Colony-Stimulating Factor; D002439:Cefotaxime; C033356:cefodizime