High-resolution FISH mapping of beta-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in ffttle standard karyotypes.
High-resolution chromosomal fluorescence in situ hybridization (FISH) and R-banding allowed the chromosomal localization of the beta-defensin gene cluster (DEFB@) to chromosome band 1p12 in the river buffalo (Bubalus bubalis, 2n = 50) and to band 24q13 in sheep (Ovis aries, 2n = 54), according to standard nomenclatures. The localizations permitted the tentative assignment of the entire bovine syntenic group U25 to these chromosomes. A FISH mapping control on cattle chromosomes confirmed the localization of DEFB@ in the homoeologous chromosome and chromosome band. Comparisons were made between river buffalo chromosome 1p and the homoeologous cattle chromosome on standard karyotypes; the results are discussed in light of gene assignments, extensive chromosome band conservation among the Bovidae, and inconsistencies in domestic cattle chromosome nomenclatures.
Iannuzzi
L
L
National Research Council (CNR), IABBAM, Naples, Italy.
Gallagher
D S
DS
Di Meo
G P
GP
Diamond
G
G
Bevins
C L
CL
Womack
J E
JE
D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D023082:Defensins; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D005810:Multigene Family; D012756:Sheep / Q000235:genetics*; D009626:Terminology as Topic
D001798:Blood Proteins; D023082:Defensins; D005819:Genetic Markers