Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector containing the CD4 gene.
It has been reported recently that CD4 is a major component of the receptor for human herpesvirus 7 (HHV-7), which has been newly identified as a T-lymphotropic virus. To investigate further the role of CD4 in HHV-7 infection, we examined the susceptibility to HHV-7 infection of various CD4-negative or weakly positive cell lines into which the cDNA for CD4 was transferred using an adenovirus vector (Adex1CACD4). Of 13 cell lines transduced with Adex1CACD4, including T-lymphoid, B-lymphoid, monocytoid, and myeloid cell lines, one T-lymphoid cell line, one monocytoid cell line, and two cell lines established from the blast crisis of chronic myelogenous leukemia showed high susceptibility to HHV-7 infection. Taken together with the results of previous studies, these data suggest strongly that CD4 is a major component of the binding receptor for HHV-7. This study also shows that HHV-7 may be able to infect CD4-positive hematopoietic precursor cells as well as T lymphocytes.
Yasukawa
M
M
First Department of Internal Medicine, Ehime University School of Medicine, Japan. yasukawa@m.ehime-u.ac.jp
Inoue
Y
Y
Ohminami
H
H
Sada
E
E
Miyake
K
K
Tohyama
T
T
Shimada
T
T
Fujita
S
S
D000256:Adenoviridae; D015704:CD4 Antigens / Q000235:genetics*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006566:Herpesviridae Infections; D016199:Herpesvirus 7, Human; D006801:Humans; D008214:Lymphocytes / Q000821:virology*
D015704:CD4 Antigens