title: Protein disulfide isomerase and newly synthesized procollagen chains form higher-order structures in the lumen of the endoplasmic reticulum.
abstract: A number of proteins that act as necessary catalysts for correct protein folding and oligomerization in the endoplasmic reticulum (ER) are known to be retained in the organelle via the KDEL-receptor mediated retrieval mechanism. However, a complementary system that may help to retain these proteins in the organelle lumen has been suggested to exist and likely involves physical protein-protein interactions at the level of endoplasmic reticulum (ER) itself. In this report, we provide both morphological and biochemical evidence in support of this proposal. We show that in collagen-secreting human skin fibroblasts, protein disulfide isomerase and newly synthesized procollagen chains exist predominantly in an "aggregated" state, and form a reticular-like matrix in the ER lumen in vivo. The size of the aggregates was found to be variable, and may exceed 1.5 million Da. Aggregate formation appeared to be transient and to involve multiple types of protein-protein interactions, including formation of aberrant disulfide bonds. Association of protein disulfide isomerase, on the other hand, was found to require at least partly function-related disulfide bonds. These results support the existence of a reticular-like matrix in the ER lumen, and suggest that aggregation may be part of the normal maturation pathway during collagen biosynthesis.
authors.affiliation: Department of Anatomy, University of Oulu, Oulu FIN-90220, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Kellokumpu
authors.forename: M
authors.initials: M
authors.lastname: Suokas
authors.forename: L
authors.initials: L
authors.lastname: Risteli
authors.forename: R
authors.initials: R
authors.lastname: Myllylä
mesh_terms: D000911:Antibodies, Monoclonal; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007535:Isomerases / Q000032:analysis / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D009685:Nuclear Envelope / Q000187:drug effects / Q000648:ultrastructure; D011347:Procollagen / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases; D012867:Skin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011347:Procollagen; D000001:Calcimycin; D007535:Isomerases; D019704:Protein Disulfide-Isomerases