Non-NMDA receptor-mediated modulation of voltage-activated outward currents in chick neurones.
The effect of kainate on the voltage-activated current of cultured embryonic chick telencephalic neurones was studied by whole-cell voltage clamp recording. In addition to opening non-NMDA receptor coupled ion channels, kainate produced additional effects, with a slower time course: it modulated voltage-activated currents. These effects were blocked by the non-NMDA receptor antagonists CNQX (6-cyano-7-nitroquinoxaline-2,3-dione) and GYKI 53784 ((-)-1-(4-aminophenyl)-3- methylcarbamoyl-4-methyl-7,8-methylenedioxy-3,4- dihydro-5H-2,3-benzodiazepine), indicating that non NMDA receptors are involved. It has been shown that the reversible inhibition of outward potassium currents was partly due to a decrease of current amplitude and partly to a marked shift of the inactivation curve towards more negative potentials.
Mike
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Department of Pharmacology, Hungarian Academy of Sciences, Budapest, Hungary.
Dyatlov
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Vizi
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D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002645:Chickens; D007473:Ion Channels / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D013687:Telencephalon / Q000187:drug effects*
D007473:Ion Channels; D007608:Kainic Acid