The mammalian brain rsec6/8 complex.
rsec6 and rsec8 are two components of a 17S complex in mammalian brain that is homologous to the yeast 834 kDa Sec6/8/15 complex which is essential for exocytosis. Purification and partial amino acid sequencing of the mammalian rsec6/8 complex reveals that it is composed of eight novel proteins with a combined molecular weight of 743 kDa. The complex is broadly expressed in brain and displays a plasma membrane localization in nerve terminals. Membrane associated rsec6/8 complex coimmunoprecipitates with syntaxin, a plasma membrane protein critical for neurotransmission. These data suggest a role for the mammalian rsec6/8 complex in neurotransmitter release via interactions with the core vesicle docking and fusion apparatus.
Hsu
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SC
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical School, California 94305-5428, USA.
Ting
A E
AE
Hazuka
C D
CD
Davanger
S
S
Kenny
J W
JW
Kee
Y
Y
Scheller
R H
RH
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010449:Peptide Mapping; D011233:Precipitin Tests; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution
D002352:Carrier Proteins; C096102:Exoc3 protein, rat; C076326:Exoc4 protein, rat; D008565:Membrane Proteins; D011506:Proteins; D050765:Qa-SNARE Proteins