Monoclonal antibody against bovine Lactoferricin and its epitopic site.
Bovine lactoferricin (LFcin B) is a strong antimicrobial peptide derived from N-lobe of lactoferrin. To study the immunochemical and structural properties of LFcin B, monoclonal antibody (mAb) was prepared and the amino acid sequence concerning with the binding to mAb has been identified. Mice injected with LFcin B showed no production of antibody specific to this peptide, whereas those with LFcin B-KLH conjugate produced anti-LFcin B antibodies. None of the mAb reacted with bovine lactoferrin C-lobe, human lactoferrin or LFcin H. By the reactivity of the mAb against the peptides synthesized on cellulose membranes using SPOTs and against chemically modified derivatives of LFcin B, the antigenic determinant of LFcin B was identified to be the sequence of "QWR".
Shimazaki
K
K
Dairy Science Laboratory, Animal Science Department, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Nam
M S
MS
Harakawa
S
S
Tanaka
T
T
Omata
Y
Y
Saito
A
A
Kumura
H
H
Mikawa
K
K
Igarashi
I
I
Suzuki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D002417:Cattle; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007781:Lactoferrin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D000939:Epitopes; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C082171:lactoferricin B; D007781:Lactoferrin