Automated high-performance liquid chromatographic separation with spectrofluorometric detection of a malondialdehyde-thiobarbituric acid adduct in plasma.
An automated HPLC separation method for the detection of malondialdehyde (MDA) in plasma samples of a healthy population is described. The procedure involves a first step of acidic hydrolysis and complex formation with thiobarbituric acid (TBA), then a protein precipitation step, and finally the separation of TBA-MDA adducts through a complete HPLC apparatus with spectrofluorometric quantification. This procedure is very useful for a routine laboratory because of its rapidity (tR = 1.7 min), simplicity and applicability, and the method gives very good results with respect to linearity (0.1-12 mumol/l), precision (within-assay C.V.%: 8, between-assay C.V.%: 10) and accuracy (recovery average: 91.66%).
Londero
D
D
Institute of Clinical Chemistry, Azienda Ospedaliera S.M.M., Udine, Italy.
Lo Greco
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D015227:Lipid Peroxidation; D008875:Middle Aged; D012015:Reference Standards; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis* / Q000378:metabolism
D007202:Indicators and Reagents; D017392:Thiobarbituric Acid Reactive Substances