Effect of reaction temperature on the determination of rat serum IgE by the avidin-biotin method.
We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE was determined by the avidin-biotin method at 4 degrees C or 37 degrees C, OVA-specific IgE peaks were detected at 37 degrees C, even with nonimmunized rats, but not at 4 degrees C.
Yamada
K
K
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Nakao
A
A
Takasugi
M
M
Itoh
Y
Y
Hassan
N A
NA
Sugano
M
M
D000818:Animals; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006882:Hydroxyapatites; D007073:Immunoglobulin E / Q000032:analysis* / Q000302:isolation & purification; D007202:Indicators and Reagents; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D013696:Temperature
D006882:Hydroxyapatites; D007202:Indicators and Reagents; D001360:Avidin; D007073:Immunoglobulin E; D001710:Biotin; D010047:Ovalbumin