title: Vitamin B12 repression of the btuB gene in Salmonella typhimurium is mediated via a translational control which requires leader and coding sequences.
abstract: Expression of the Salmonella typhimurium btuB gene, which encodes an outer membrane protein required for vitamin B12 uptake, is repressed by the presence of external vitamin B12. We have, by means of a mutational analysis, investigated which btuB sequences are required for repression. Analysis of btuB::lacZ transcriptional and translational fusions of various lengths showed that the control was exerted mainly at the translational level and required both coding and leader sequences in the btuB transcript. Regulatory mutants with a B12 non-repressible phenotype were isolated and the mutations were shown to be located at several sites within the btuB leader. Analysis of constructs carrying site-directed point mutations, which either destabilized or restabilized a putative RNA hairpin that sequesters the btuB ribosomal binding site, demonstrated that this hairpin was essential for normal repression. Comparison of the S. typhimurium btuB gene with the previously characterized S. typhimurium cbiA and Escherichia coli btuB genes reveals significant similarities as well as differences in the cis-acting sequences required for repression.
authors.affiliation: Department of Microbiology, Uppsala University, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Ravnum
authors.forename: D I
authors.initials: DI
authors.lastname: Andersson
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D001483:Base Sequence; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D014176:Protein Biosynthesis; D012331:RNA, Fungal; D012333:RNA, Messenger; D018000:Receptors, Peptide / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; C044592:BtuB protein, E coli; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D018000:Receptors, Peptide; D014805:Vitamin B 12