Study on bovine serum albumin recovery from reversed micellar solutions.
From near-infrared spectra, it was confirmed that CTAB/hexanol-octane reversed micellar solution is a suitable medium for BSA solubilization. The effects of various factors, e.g., PH, and the type and concentration of salt in stripping solution, on Bovine Serum Albumin (BSA) back extraction were studied. The suitable conditions for stripping have been ascertained, i.e., 1.0-2.0 mol/L KBr, pH = 4.3-4.9. Spectroscopic studies showed that no significant change is observed in the structure of the main chain of BSA after extraction and back extraction. In addition, the problem of how long the reversed micellar solution can be recycled has been examined. Finally, the recovery and concentration of protein were successfully obtained with suitable phase ratio.
Lu
Q
Q
Chemical Engineering Research Center, East China University of Science and Technology, Shanghai, China.
Li
K
K
Shi
Y
Y
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D003902:Detergents; D000441:Hexanols; D008823:Micelles; D009795:Octanes; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D002593:Cetrimonium Compounds; D003902:Detergents; D000441:Hexanols; D008823:Micelles; D009795:Octanes; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D000077286:Cetrimonium