title: The Cu,Zn superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration. Evidence for altered subunit interaction in all the bacteriocupreins.
abstract: Gel-filtration chromatography experiments performed at high protein concentrations demonstrate that the Cu,Zn superoxide dismutase from Escherichia coli is monomeric irrespective of the buffer and of ionic strength. The catalytic activity of the recombinant enzyme is comparable with that of eukaryotic isoenzymes, indicating that the dimeric structure commonly found in Cu,Zn superoxide dismutases is not necessary to ensure efficient catalysis. The analysis of the amino acid sequences suggests that an altered interaction between subunits occurs in all bacterial Cu,Zn superoxide dismutases. The substitution of hydrophobic residues with charged ones at positions located at the dimer interface of all known Cu,Zn superoxide dismutases could be specifically responsible for the monomeric structure of the E. coli enzyme.
authors.affiliation: Department of Biology, University of Rome Tor Vergata, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Battistoni
authors.forename: S
authors.initials: S
authors.lastname: Folcarelli
authors.forename: R
authors.initials: R
authors.lastname: Gabbianelli
authors.forename: C
authors.initials: C
authors.lastname: Capo
authors.forename: G
authors.initials: G
authors.lastname: Rotilio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D017931:DNA Primers; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017421:Sequence Analysis; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; C030389:bacteriocuprein; D013482:Superoxide Dismutase