title: An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue.
abstract: A novel protein harboring a 280-amino acid region from an Alu-linked repetitive sequence (bovine Alu-like dimer-driven family) was isolated from a bovine brain S-100 fraction using monoclonal antibodies against a rat GTPase-activating protein that shares the same epitope. The protein has an apparent molecular mass of 97 kDa (p97). Western blot analysis using extracts prepared from various tissues showed p97 to be predominantly detected in brain and moderately in liver and lung. From sequence analysis of the cDNA encoding p97, it was found that the 840-base pair sequence homologous to a part of the bovine Alu-like dimer-driven family, which has never been shown to be expressed, occurs in the middle of the protein coding region. The protein also contains a pair of intramolecular repeats composed of 40 highly hydrophilic amino acids at the C terminus. Human cDNA homologous to p97 was cloned, and its nucleotide sequence demonstrates that the 840-base pair repetitive sequence and one of the intramolecular repeats are missing. We named p97 bovine BCNT after Bucentaur. These results show that bovine BCNT is a unique molecule and suggest that an analysis of the relationship between bovine bcnt and its human homologue may help further the understanding of gene organization and evolution.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, Tokyo 194.
authors.forename: T
authors.initials: T
authors.lastname: Nobukuni
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: A
authors.initials: A
authors.lastname: Omori
authors.forename: S
authors.initials: S
authors.lastname: Ichinose
authors.forename: T
authors.initials: T
authors.lastname: Iwanaga
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Hattori
authors.forename: K
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Miyata
authors.forename: T
authors.initials: T
authors.lastname: Masui
authors.forename: S
authors.initials: S
authors.lastname: Iwashita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D016384:Consensus Sequence; D018076:DNA, Complementary; D000939:Epitopes / Q000032:analysis; D020690:GTPase-Activating Proteins; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins; D010750:Phosphoproteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000276:immunology; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; C104422:CFDP1 protein, human; D018076:DNA, Complementary; D000939:Epitopes; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011506:Proteins; D011994:Recombinant Proteins