[Proton magnetic resonance spectroscopy of excised pig liver].
After evaluating ex vivo pig liver by 1H magnetic resonance spectroscopy (1H-MRS) using a 1.5 Tesla super-conducting unit, the assignment of peaks was reevaluated in vitro using a 9.4 Tesla superconducting unit. The portal vein was cannulated and perfused by cooled Euro-Collins solution, and pig liver was removed and preserved in the solution. Five to 8 g of the liver was excised before and after preservation, then extracted by perchloric acid (PCA). In 1H-MRS of PCA extracted pig liver, large peaks of fatty acid disappeared, while peaks of the choline group from 3.2-3.3 ppm were clearly observed. Using high performance liquid chromatography (HPLC) and 31P (phosphorus)-MRS, the 3.23 ppm peak was determined as glycerophosphorylcholine (GPC), which diminished after preservation. The chronological change of GPC in PCA extracted pig liver was able to be observed by 1H-MRS. GPC peak may play a role as an indicator in evaluating preserved liver by 1H-MRS.
Tamura
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Department of Radiology, Saitama Medical School.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005997:Glycerylphosphorylcholine / Q000302:isolation & purification; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010472:Perchlorates; D010767:Phosphorylcholine / Q000302:isolation & purification; D013552:Swine
D010472:Perchlorates; D010767:Phosphorylcholine; D005997:Glycerylphosphorylcholine