Precancerous pathology evoked by hexachlorobenzene treatment.
The porphyrinogenic and carcinogenic ability of hexachlorobenzene (HCB) was assayed in male and female gold hamsters, and histological examinations of tissue alterations were performed. So it was studied, in liver: a) porphyrin content which was significantly increased at five months of HCB treatment, specially in males, and the pattern of accumulated porphyrins which was altered independent of the sex, b) haem pathway enzymes:delta aminolaevulinic acid synthase, ferrochelatase and porphyrinogen carboxylyase (PCL); among which only PCL appeared to be altered just at ten months of HCB feeding. While thyroid gland and kidney remained unaltered along the treatment time, liver and spleen exhibited a noticeable size variation and morphological alterations. In fact the spleen in treated animals was hypotrophic showing a red pulp less developed with respect to the Malpighian corpuscles and many macrophages with iron deposits. Respect to the liver, enlargement in size of hepatocytes, high content of iron deposits, no PAS positive structures in the cytoplasm, several small lipid droplets, microsteatosis although no cytonecrosis, polymorphic nuclei, and proliferations of nucleoli were observed. Therefore HCB is able to cause precancerous pathology and to induce porphyria in hamsters, but not hyperthyroidism, upon this experimental conditions. By the way, males were found to be a good experimental model, better than females, to study the earliest relations between porphyria and cancer.
Ríos de Molina
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MC
Departamento de Química Biológica, Universidad de Buenos Aires, Argentina.
Iglesias
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S
Lauría
L
L
San Martín de Viale
L C
LC
D000818:Animals; D006224:Cricetinae; D005260:Female; D006418:Heme / Q000378:metabolism; D006581:Hexachlorobenzene / Q000494:pharmacology / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D009929:Organ Size; D011166:Porphyrins / Q000032:analysis*; D013154:Spleen / Q000187:drug effects* / Q000473:pathology; D013961:Thyroid Gland / Q000187:drug effects
D011166:Porphyrins; D006418:Heme; D006581:Hexachlorobenzene