High-performance liquid chromatographic determination of baclofen in human plasma.
A reversed-phase isocratic HPLC method is described for the determination of baclofen in human plasma. Solid-phase extraction using a SCX Bond Elut column is used followed by derivatization with o-phthalaldehyde-tert.-butanethiol and electrochemical detection. Both the within- and between-day R.S.D. and inaccuracy are less than 10% and 7%, respectively, even at the limit of quantification of the method, i.e., 10 ng/ml. The method was shown to give optimum performance in terms of sensitivity, precision and accuracy for the pharmacokinetic study of baclofen after a single oral administration to volunteers.
Millerioux
L
L
Cephac Research Centre, Saint-Benoît, France.
Brault
M
M
Gualano
V
V
Mignot
A
A
D001418:Baclofen / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D018755:GABA Agonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D018755:GABA Agonists; D007202:Indicators and Reagents; D001418:Baclofen