Structural and functional characterization of three human immunoglobulin kappa light chains with different pathological implications.
The structural properties of three immunoglobulins light chains: kappa SCI, responsible for light chain deposition disease (Bellotti, V., Stoppini, M., Merlini, G., Zapponi, M.C., Meloni, M.L., Banfi, G. and Ferri, G. (1991) Biochim. Biophys. Acta 1097, 177-182), k INC responsible for light chain amyloidosis (Ferri, G., Stoppini, M., Iadarola, P., Bellotti, V. and Merlini, G. (1989) Biochim. Biophys. Acta 995, 103-108) and the non-pathogenic kappa MOS were analyzed by fluorescence spectroscopy and circular dichroism. Comparative evaluation of the data shows that SCI and MOS have similar stability under different conditions, while the amyloid k INC behaves as a very unstable protein. As calculated from the GdnHCl curves, the midpoint of unfolding transition was 1.35 M for SCI, 1.20 M for MOS and 0.1 M for INC. Analysis of CD spectra evidences that the three proteins conserve their conformation in the range of pH 4-8. Change in temperature at pH 4.0 produces the premature transition of INC (Tm 40 degrees C) with respect to SCI and MOS (Tm 50 degrees C). At this pH both the pathological SCI and INC light chains aggregate at a temperature of 20 degrees C lower than the normal counterpart. The specific kidney deposition of kappa SCI has been evidenced after injection of the 125I labelled light chain into mice. No deposition was detectable in the case of INC and MOS.
Bellotti
V
V
Department of Biochemistry University of Pavia, Italy. dbioc@unipv.it
Stoppini
M
M
Mangione
P P
PP
Fornasieri
A
A
Min
L
L
Merlini
G
G
Ferri
G
G
D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000209:etiology*; D000818:Animals; D002942:Circular Dichroism; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011485:Protein Binding; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D014018:Tissue Distribution
D007145:Immunoglobulin kappa-Chains