Treponema medium sp. nov., isolated from human subgingival dental plaque.
A new Treponema species, for which we propose the name Treponema medium, was isolated from subgingival plaque from an adult with periodontal disease. The morphological characteristics, differential biochemical characteristics, and protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of this organism are described. The guanine-plus-cytosine content of the DNA of T. medium is 51 mol%. The levels of DNA-DNA relatedness of the new species to other Treponema species, including Treponema denticola, Treponema vincentii, Treponema socranskii, Treponema pallidum, and Treponema phagedenis, are less than 30%. A phylogenetic analysis based on 16S rRNA sequences distinguished the new Treponema strain from strains belonging to previously described Treponema species. The type strain of T. medium is strain G7201.
Umemoto
T
T
Department of Oral Microbiology, School of Dentistry, Asahi University, Gifu, Japan. toshiut@hatelecom.or.jp
Nakazawa
F
F
Hoshino
E
E
Okada
K
K
Fukunaga
M
M
Namikawa
I
I
D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D001482:Base Composition; D015153:Blotting, Western; D003470:Culture Media / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014208:Treponema / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S