Methods and applications of three-color confocal imaging.
A simple method for constructing two- and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confocal microscopy.
Paddock
S W
SW
Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA. paddock@facstaff.wisc.edu
Hazen
E J
EJ
De Vries
P J
PJ
D000818:Animals; D004330:Drosophila / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D012984:Software; D014743:Videotape Recording