Effect of acute magnesium deficiency (MgD) on aortic endothelial cell (EC) oxidant production.
Magnesium deficiency (MgD) has been associated with production of reactive oxygen species, cytokines, and eicosanoids, as well as vascular compromise in vivo. Although MgD-induced inflammatory change occurs during "chronic" MgD in vivo, acute MgD may also affect the vasculature and consequently, predispose endothelial cells (EC) to perturbations associated with chronic MgD. As oxyradical production is a significant component of chronic MgD, we examined the effect of acute MgD on EC oxidant production in vitro. In addition we determined EC; pH, mitochondrial function, lysosomal integrity and general cellular antioxidant capacity. Decreasing Mg2+ (< or = 250microM) significantlyincreased EC oxidant production relative to control Mg2+ (1000microM). MgD-induced oxidant production, occurring within 30min, was attenuated by EC treatment with oxyradical scavengers and inhibitors of eicosanoid biosynthesis. Coincident with increased oxidant production were reductions in intracellular glutathione (GSH) and corresponding EC alkalinization. These data suggest that acute MgD is sufficient for induction of EC oxidant production, the extent of which may determine, at least in part, the extent of EC dysfunction/injury associated with chronic MgD.
Wiles
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ME
The George Washington University Medical Center, Division of Experimental Medicine, Washington, D.C., USA. mwiles@nexstar.com
Wagner
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TL
Weglicki
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WB
D000208:Acute Disease; D000818:Animals; D001011:Aorta; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D015777:Eicosanoids / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005452:Fluoresceins / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism; D008275:Magnesium Deficiency / Q000378:metabolism*; D008928:Mitochondria / Q000502:physiology; D009499:Neutral Red / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D004396:Coloring Agents; D015777:Eicosanoids; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D012235:Rhodamines; D020112:Rhodamine 123; D009499:Neutral Red; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione