[Automation on membranes: countercurrent isotachophoresis and immunoelectrochromatography].
The review discusses two related methods--counterflow isotachophoresis and immunoaffinity electrochromatography on porous membranes developed by the authors. The methods exploit the peculiarity of electroendosmosis in porous membranes at the conditions of discontinues electrophoresis. There are described the principles and application of the above methods for automatic performance of electrophoretic analysis of low-protein biological fluids, of western blotting, epitope analysis of monoclonal antibodies and multiantigen assays. The possibility of DNA-binding protein detection is also considered.
Abelev
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GI
Karamova
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ER
D001331:Automation; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003378:Counterimmunoelectrophoresis / Q000295:instrumentation / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate