title: Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.
abstract: A cDNA encoding a subunit of the verrucotoxin (VTX) has been identified from a cDNA library derived from stonefish venom glands. It encodes a polypeptide of 708 amino-acid residues, followed by a 3'-untranslated region of 895 bp long. The ORF contains the complete mature sequence of the beta-subunit of the VTX, as inferred from both the presence of an identical N-terminus sequence and 96% homology among the 506 amino terminus residues found in the partial sequence of the beta-subunit of the stonustoxin from Synanceia horrida (Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., and Yuen, R. (1994) Toxicon 32, 1684-1688). Upstream the mature sequence, we noticed the presence of an incomplete peptide of a 13 amino acids, whose unusual primary structure supports the idea of the existence of a propeptide and/or of a new secretion signal.
authors.affiliation: Laboratoire de Biologie et Contrôle des Organismes parasites (Equipe Toxines et Insectes), Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
authors.forename: P
authors.initials: P
authors.lastname: Garnier
authors.forename: F
authors.initials: F
authors.lastname: Ducancel
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: J C
authors.initials: JC
authors.lastname: Boulain
authors.forename: F
authors.initials: F
authors.lastname: Goudey-Perrière
authors.forename: C
authors.initials: C
authors.lastname: Perrière
authors.forename: A
authors.initials: A
authors.lastname: Ménez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005397:Fish Venoms / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D005397:Fish Venoms; D006023:Glycoproteins; C094408:verrucotoxin protein, Synanceia verrucosa