title: Crystal structure of a coagulogen, the clotting protein from horseshoe crab: a structural homologue of nerve growth factor.
abstract: The clotting cascade system of the horseshoe crab (Limulus) is involved in both haemostasis and host defence. The cascade results in the conversion of coagulogen, a soluble protein, into an insoluble coagulin gel. The clotting enzyme excises the fragment peptide C from coagulogen, giving rise to aggregation of the monomers. The crystal structure of coagulogen reveals an elongated molecule that embraces the helical peptide C fragment. Cleavage and removal of the peptide C would expose an extended hydrophobic cove, which could interact with the hydrophobic edge of a second molecule, leading to a polymeric fibre. The C-terminal half of the coagulogen molecule exhibits a striking topological similarity to the neurotrophin nerve growth factor (NGF), providing the first evidence for a neurotrophin fold in invertebrates. Similarities between coagulogen and Spatzle, the Drosophila ligand of the receptor Toll, suggest that the neurotrophin fold might be considered more ancient and widespread than previously realized.
authors.affiliation: Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Martinsried, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bergner
authors.forename: V
authors.initials: V
authors.lastname: Oganessyan
authors.forename: T
authors.initials: T
authors.lastname: Muta
authors.forename: S
authors.initials: S
authors.lastname: Iwanaga
authors.forename: D
authors.initials: D
authors.lastname: Typke
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: W
authors.initials: W
authors.lastname: Bode
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006737:Horseshoe Crabs / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000737:chemistry*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D001798:Blood Proteins; D009414:Nerve Growth Factors; C013445:coagulogen