Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production.
We report essentially complete 1H NMR assignments for the capsular polysaccharides from Neisseria meningitidis serotypes A, C, W-135, and Y. These polysaccharides are components of current polysaccharide vaccines against meningococcal infection and of the polysaccharide-protein conjugate vaccines under development. From these NMR data the pattern of O-acetylation was determined. O-Acetylation of the W-135 polysaccharide is reported for the first time. We also show that, for the Types C and W-135 polysaccharides a migration of O-acetyl groups occurs during storage in solution, and demonstrate that high field 1H NMR represents a simple and sensitive method to define the O-acetylation pattern of individual batches of these polysaccharides.
Lemercinier
X
X
Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Herts, UK.
Jones
C
C
D000107:Acetylation; D016667:Bacterial Capsules / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009345:Neisseria meningitidis / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping; D014612:Vaccines / Q000276:immunology
D011135:Polysaccharides, Bacterial; D014612:Vaccines