title: Complete synthesis of 3'-sialyl-N-acetyllactosamine by regioselective transglycosylation.
abstract: Transglycolytic synthesis of 3'-sialyl-N-acetyllactosamine by sequential use of beta-galactosidase from Bacillus circulans and trans-sialidase from Trypanosoma cruzi was described. These reactions depicted the first complete synthesis of a biologically important oligosaccharide with high regioselectivity avoiding use of glycosyltransferases and NDP sugars.
authors.affiliation: Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, Italy. vetere@univ.trieste.it
authors.forename: A
authors.initials: A
authors.lastname: Vetere
authors.forename: S
authors.initials: S
authors.lastname: Paoletti
mesh_terms: D000606:Amino Sugars / Q000096:biosynthesis*; D000818:Animals; D001407:Bacillus / Q000201:enzymology / Q000378:metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D008969:Molecular Sequence Data; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000606:Amino Sugars; C032269:sialyl-N-acetyllactosamine; D001616:beta-Galactosidase