Identification and characterization of receptor for dengue virus-induced macrophage cytotoxin.
The present study was undertaken to identify the receptor for dengue virus type 2 (DV) induced macrophage cytotoxin (CF2) on mouse peritoneal macrophages (MPhi). The binding of 125I-labelled CF2 to MPhi was saturable (15 nM), reversible, temperature, pH- and time-dependent. The saturation concentration was similar to that causing cell death. Scatchard analysis showed the presence of intermediate type of affinity receptor and the number of receptor sites was 1.1 x 10(6) per cell with dissociation constant of 14.28 nM.
Khare
P D
PD
Postgraduate Department of Microbiology, K.G.Medical College, Lucknow, India.
Dhawan
R
R
Chaturvedi
U C
UC
D000818:Animals; D003603:Cytotoxins / Q000096:biosynthesis*; D003716:Dengue Virus; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011991:Receptors, Virus / Q000032:analysis*
D003603:Cytotoxins; D011991:Receptors, Virus