Rapid uncoupling of oxidative phosphorylation accompanies glutamate toxicity in rat cerebellar granule cells.
A 100 microM glutamate pulse administered to rat cerebellar granule cells causes a very rapid and progressive decrease in both cell and mitochondrial oxygen consumption caused by glucose and succinate addition, respectively. The respiratory control ratio, which reflects the ability of mitochondria to produce ATP, is reduced by 50% within the first 30 min after glutamate addition. Subsequent to glutamate exposure, a progressive decrease of respiratory control ratio to almost 1 was found within the following 3-5 h. The addition of extra calcium had no effect per se on oxygen consumption by cell homogenate.
Atlante
A
A
Centro di Studio sui Mitocondri e Metabolismo Energetico, C.N.R., Bari, Italy.
Gagliardi
S
S
Minervini
G M
GM
Marra
E
E
Passarella
S
S
Calissano
P
P
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002531:Cerebellum / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000633:toxicity*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D018698:Glutamic Acid; D005947:Glucose