Evaluation of a monoclonal antibody-based sandwich-ELISA for detection of circulating Schistosoma japonicum antigen (Sj31/32) in an endemic area of China.
A sandwich-ELISA specific for 31/32 kDa schistosome proteins was used to trace infections in a Chinese population living in a low endemicity area for schistosomiasis japonica. The study population included 304 persons for whom records were available from 1992 onwards. In 1994, 2 individuals were positive in the miracidial hatching assay performed with 30 g of stools. The ELISA gave positive results for 8 individuals, including these 2 with active infections. Of the remaining 6 ELISA-positive, 5 had excreted eggs and received praziquantel in 1993 and may have harboured residual worms in 1994. It is concluded that this ELISA is able, in a low prevalence situation, to identify the few infected individuals.
Li
Y L
YL
Department of Parasitology, Tongji Medical University, Wuhan, PR China.
Liu
S Y
SY
Han
J J
JJ
Ning
C X
CX
Ruppel
A
A
D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012549:Schistosoma japonicum / Q000254:growth & development / Q000302:isolation & purification*; D012554:Schistosomiasis japonica / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth