title: Unsupervised, automated segmentation of the normal brain using a multispectral relaxometric magnetic resonance approach.
abstract: The purpose of this study was the development and testing of a method for unsupervised, automated brain segmentation. Two spin-echo sequences were used to obtain relaxation rates and proton-density maps from 1.5 T MR studies, with two axial data sets including the entire brain. Fifty normal subjects (age range, 16 to 76 years) were studied. A Three-dimensional (3D) spectrum of the tissue voxels was used for automatic segmentation of gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) and for calculation of their volumes. Accuracy and reproducibility were tested with a three-compartment phantom simulating GM, WM, and CSF. In the normal subjects, a significant decrease of GM fractional volume and increased CSF volume with age were observed (P < 0.0001), with no significant changes in WM. This multispectral segmentation method permits reproducible, operator-independent volumetric measurements.
authors.affiliation: Department of Radiology, CNR-Nuclear Medicine Center, University Frederico II, Naples, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Alfano
authors.forename: A
authors.initials: A
authors.lastname: Brunetti
authors.forename: E M
authors.initials: EM
authors.lastname: Covelli
authors.forename: M
authors.initials: M
authors.lastname: Quarantelli
authors.forename: M R
authors.initials: MR
authors.lastname: Panico
authors.forename: A
authors.initials: A
authors.lastname: Ciarmiello
authors.forename: M
authors.initials: M
authors.lastname: Salvatore
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology; D001921:Brain / Q000033:anatomy & histology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D019047:Phantoms, Imaging; D015203:Reproducibility of Results