A model for Fis N-terminus and Fis-invertase recognition.
In this modeling, we show that the elusive Fis N-terminus involved in invertase-mediated site-specific DNA inversion is, by all indications, a hinged flapping loop. The prediction is based on a combined sequence and secondary structure alignment against known structures of protein segments, as well as a tetrapeptide fragment observed crystallographically. Its validity is strongly supported by the ability to interpret consistently the available mutagenesis data pertaining to this region including, especially, a series of deletion mutants which until this work had been a puzzle in the search for structural explanations. A model for Fis-invertase recognition is also proposed.
Tzou
W S
WS
Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
Hwang
M J
MJ
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035021:Factor For Inversion Stimulation Protein; D006026:Glycoside Hydrolases / Q000378:metabolism*; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011485:Protein Binding; D043324:beta-Fructofuranosidase
D002352:Carrier Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase