Detection of canine herpesvirus 1 in a wide range of tissues using the polymerase chain reaction.
Canine herpesvirus 1 (CHV-1), a member of the alphaherpesvirus sub-family, is known to cause fatal infections in litters of puppies and may also be involved in infertility, abortion, and stillbirths in adult dogs. The purpose of this study was to determine the presence of CHV-1 DNA using the polymerase chain reaction (PCR) in twelve key sites that have been associated with latency for the other herpesviruses. A 605 base pair portion of the viral glycoprotein B (gB) gene was amplified using degenerate primers, cloned, and sequenced. Conventional 20 mer primers were designed using this sequence information to amplify a 120 bp fragment of gB situated between the original degenerate primers. The specificity of amplification was confirmed by Southern Blot hybridisation using an internal oligonucleotide probe. DNA was extracted from tissue samples taken from twelve dogs at post mortem and from twenty-four blood samples. Nine out of twelve dogs showed evidence of infection with CHV-1; the tissues most commonly affected were lumbo-sacral ganglia (5/12 dogs), tonsil (5/12), parotid salivary gland (4/9), and liver (4/9). No positive results were detected within the twenty-four blood samples. These results indicate that exposure to CHV-1 may be much more common than previously suggested.
Burr
P D
PD
Department of Veterinary Pathology, University of Glasgow, UK. p.burr@udcf.gla.cu.uk
Campbell
M E
ME
Nicolson
L
L
Onions
D E
DE
D000818:Animals; D015139:Blotting, Southern; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D005727:Ganglia, Spinal / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002184:Herpesvirus 1, Canid / Q000302:isolation & purification*; D008099:Liver / Q000821:virology; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D014066:Palatine Tonsil / Q000821:virology; D010306:Parotid Gland / Q000821:virology; D010920:Placenta / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D012756:Sheep; D014759:Viral Envelope Proteins / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus