title: Effect of hydromechanical stress on cellular antigens of Bordetella pertussis.
abstract: Cells of Bordetella pertussis grown in a bioreactor under stirring conditions were studied to investigate the effect of shear stress on cellular-bound filamentous haemagglutinin (FHA). FHA attached to the bacterial surface, unlike extracellular FHA, was not affected at the shear levels tested. Moreover, no other cellular immunogen involved in the whole-cell protective activity seemed to be affected by hydromechanical forces.
authors.affiliation: Centro de Investigación y Desarrollo de Fermentaciones Industriales (CINDEFI), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Rodriguez
authors.forename: D F
authors.initials: DF
authors.lastname: Hozbor
authors.forename: O M
authors.initials: OM
authors.lastname: Yantorno
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001422:Bacterial Adhesion; D019149:Bioreactors; D001886:Bordetella pertussis / Q000254:growth & development / Q000276:immunology*; D006367:HeLa Cells; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology*; D013314:Stress, Mechanical; D014611:Vaccination; D014917:Whooping Cough / Q000517:prevention & control
chemical_list: D000942:Antigens, Bacterial; D006388:Hemagglutinins; D010567:Pertussis Vaccine