title: Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique.
abstract: This study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology.
authors.affiliation: Centre for Electron Microscopy and Microstructure Analysis, University of Adelaide, South Australia.
authors.forename: M
authors.initials: M
authors.lastname: Henderson
authors.forename: R
authors.initials: R
authors.lastname: Polewski
authors.forename: J C
authors.initials: JC
authors.lastname: Fanning
authors.forename: M A
authors.initials: MA
authors.lastname: Gibson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D003285:Contractile Proteins / Q000276:immunology / Q000378:metabolism*; D005123:Eye / Q000378:metabolism / Q000648:ultrastructure; D000071837:Fibrillins; D005455:Fluorescent Antibody Technique; D008840:Microfilament Proteins / Q000378:metabolism*; D008854:Microscopy, Electron
chemical_list: D000911:Antibodies, Monoclonal; D003285:Contractile Proteins; D000071837:Fibrillins; D008840:Microfilament Proteins