title: Susceptibility of Pseudomonas aeruginosa isolates to ceftazidime is unrelated to the expression of the outer membrane protein OprC.
abstract: Previously, it has been postulated that the porin OprC facilitates the diffusion of ceftazidime through the outer membrane of Pseudomonas aeruginosa. To further investigate this claim, the outer membrane protein (OMP) profiles of 22 ceftazidime-susceptible clinical isolates were analyzed. No correlation was found between MIC values and the level of expression of OprC. Further, OprC was either undetectable or expressed in reduced amounts in 12 isolates. In contrast, OprF and OprE were present in all isolates studied. This study suggests that OprC is dispensable for the permeation of ceftazidime through the outer membrane of P. aeruginosa.
authors.affiliation: Department of Microbiology, School of Medicine, University Hospital, Valencia, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Pérez
authors.forename: D
authors.initials: D
authors.lastname: Navarro
authors.forename: C
authors.initials: C
authors.lastname: Gimeno
authors.forename: J
authors.initials: J
authors.lastname: García-de-Lomas
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins; D002442:Ceftazidime / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D018272:Porins / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000187:drug effects*; D013030:Spain; D013845:Thienamycins / Q000494:pharmacology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002511:Cephalosporins; C095016:OprC protein, Pseudomonas aeruginosa; D018272:Porins; D013845:Thienamycins; D015378:Imipenem; D002442:Ceftazidime