title: [A novel method of concentrating bacterial suspensions for transmission electron microscopy].
abstract: Presents a new method for making preparations of bacteria and their unculturable forms from suspensions with low concentrations of cells, from 1 x 108 to 1 x 104 per ml. to be examined under transmission electron microscope. The novelty of the method consists in the technique of concentrating bacteria by making a colloid solution consisting of bovine serum albumin and polyethyleneglycol. Associations of protein molecules, polyethyleneglycol, and bacteria, forming in the solution, are sedimented by centrifugation after 48-hour incubation at 4 degrees C. The solidity of the resultant macroscopic sediment is sufficient for studying the cells contained in it under transmission electron microscope.
authors.forename: L V
authors.initials: LV
authors.lastname: Didenko
authors.forename: N D
authors.initials: ND
authors.lastname: Konstantinova
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Romanova
authors.forename: G A
authors.initials: GA
authors.lastname: Levina
authors.forename: N V
authors.initials: NV
authors.lastname: Alekseeva
mesh_terms: D001431:Bacteriological Techniques; D008854:Microscopy, Electron; D012486:Salmonella typhimurium / Q000254:growth & development / Q000648:ultrastructure*