Protection of ischemic myocardium by inhibition of contracture in isolated rat heart.
Protection of the ischemic myocardium by pretreatment with a high dose of 2,3-butanedione monoxime (BDM) is attributed to the enhancement of glycolytic ATP production rather than to the inhibition of contracture during mild ischemia. Our objective was to investigate whether the inhibition of contracture would protect the arrested heart during prolonged ischemia. Isolated perfused rat hearts were subjected to 30 min of low-flow ischemia followed by reperfusion. Ischemic hearts were treated with BDM (5 mmol/l) after beating stopped. BDM ameliorated the increase in intraventricular pressure after ischemia without significant changes in ATP levels and with a decreased accumulation of lactate. BDM treatment accelerated the recovery of function and high-energy phosphates with reduced myocardial Ca2+ overload. The results of this study suggested that inhibition of contracture can protect the heart from ischemia-reperfusion injury.
Tani
M
M
Department of Geriatric Medicine, Keio University School of Medicine, Tokyo, Japan.
Hasegawa
H
H
Suganuma
Y
Y
Shinmura
K
K
Kayashi
Y
Y
Nakamura
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003286:Contracture / Q000517:prevention & control*; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology; D004734:Energy Metabolism; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left
D010725:Phosphocreatine; C004717:diacetylmonoxime; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D003931:Diacetyl; D002118:Calcium