Development of a SCAR marker linked to the I gene in common bean.
Two 24-mer SCAR primers (SW13) were developed from a previously identified 10-mer RAPD primer (OW13(690)) linked to the I gene, which conditions resistance to bean common mosaic virus (BCMV) in common bean. Linkage between SW13 and the I gene was tested in three F2 populations segregating for both SW13 and the I gene: N84004/Michelite (1.0 +/- 0.7 cM), Seafarer/UI-114 (1.3 +/- 0.8 cM), and G91201/Alpine (5.0 +/- 2.2 cM). SW13 proved to be more specific and reproducible than the OW13(690) RAPD marker. Using different heat-stable DNA polymerases, SW13 amplified a single 690-bp fragment linked to the I gene that more consistently permitted the identification of resistant plants. In addition, the presence of the I gene was detected using SW13 in genotypes originating from different gene pools of Phaseolus vulgaris L., indicating a broad utility of this marker for bean breeding programs.
Melotto
M
M
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824, USA.
Afanador
L
L
Kelly
J D
JD
D000483:Alleles; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry; D007887:Fabaceae / Q000235:genetics / Q000821:virology*; D005819:Genetic Markers; D009029:Mosaic Viruses / Q000235:genetics*; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction
D018744:DNA, Plant; D005819:Genetic Markers