Cloning of the 3' end of rat bax-alpha and corresponding developmental down-regulation in differentiating primary, cultured oligodendrocytes.
Bax-alpha is thought to form heterodimers with Bcl-2 and prevent apoptotic cell death. A sequence was isolated from oligodendrocyte cDNA corresponding to the uncloned 3' end of the rat bax-alpha coding region and part of the 3' UTR via a degenerate polymerase chain reaction (PCR)-based cloning method. The rat bax-alpha clone is 96 and 91% homologous to mouse and human clones, respectively, and the 3' UTR demonstrates high homology with the cloned human 3' UTR. Northern analysis demonstrated that the 1.0 kb bax-alpha mRNA species was predominant. bax-alpha mRNA is expressed in mitotic, oligodendrocyte progenitors, and is subsequently down-regulated 2-fold in differentiating oligodendrocytes.
Madison
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Department of Microbiology, MC-3205, University of Connecticut School of Medicine, Farmington 06030, USA. madison@panda.uchc.edu
Pfeiffer
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; C494827:Bax protein, mouse; C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein