Involvement of sphingolipids in the endothelin-1 signal transduction mechanism in rat brain.
In cerebral cortex, endothelin-1 (ET-1) evoked a decrease of 40% in sphingomyelin (SM) levels together with an increase in both ceramide and glycosphingolipid (GSL) levels (100 and 56% respectively). These facts indicate that ET-1 increases sphingomyelinase activity and, possibly, activates the synthesis of GSL. By contrast, in cerebellum ET-1 seems to activate the hydrolysis of both SM and GSL, since the peptide evoked a decrease (near 30%) of their levels concomitantly with an increased production of ceramides (200%). These ceramides are clearly different from those produced in cerebral cortex which come from the SM hydrolysis only. It is suggested that ETB receptor subtype is involved in these responses.
Catalán
R E
RE
Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Aragonés
M D
MD
Martínez
A M
AM
Fernández
I
I
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D006028:Glycosphingolipids / Q000378:metabolism*; D006868:Hydrolysis; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
D002518:Ceramides; D019332:Endothelin-1; D006028:Glycosphingolipids