title: Synthesis of alanine and leucine by reductive amination of 2-oxoic acid with combination of hydrogenase and dehydrogenase.
abstract: Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leucine synthesis was also carried out by the combination of NADH regeneration system and leucine dehydrogenase (LeuDH). The conversion of 4-methyl-2-oxovalerate to leucine was 60% after 1.5 h.
authors.affiliation: Department of Chemistry and Biochemistry, Numazu College of Technology, Shizuoka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Hasumi
authors.forename: K
authors.initials: K
authors.lastname: Fukuoka
authors.forename: S
authors.initials: S
authors.lastname: Adachi
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: I
authors.initials: I
authors.lastname: Okura
mesh_terms: D000409:Alanine / Q000138:chemical synthesis*; D006864:Hydrogenase; D007930:Leucine / Q000138:chemical synthesis*; D009243:NAD; D010088:Oxidoreductases
chemical_list: D009243:NAD; D010088:Oxidoreductases; D006864:Hydrogenase; D007930:Leucine; D000409:Alanine