title: Simultaneous determination of azathioprine and 6-mercaptopurine by high-performance liquid chromatography.
abstract: A specific, sensitive, single-step solid-phase extraction and reversed-phase high-performance liquid chromatographic method for the simultaneous determination of plasma 6-mercaptopurine and azathioprine concentrations is reported. Following solid-phase extraction, analytes are separated on a C18 column with mobile phase consisting of 0.8% acetonitrile in 1 mM triethylamine, pH 3.2, run on a gradient system. Quantitation limits were 5 ng/ml and 2 ng/ml for azathioprine and 6-mercaptopurine, respectively. Peak heights correlated linearly to known extracted standards for 6-mercaptopurine and azathioprine (r = 0.999) over a range of 2-200 ng/ml. No chromatographic interferences were detected.
authors.affiliation: Department of Medicine, Mayo Clinic/Mayo Foundation, Rochester, MN 59905, USA.
authors.forename: E C
authors.initials: EC
authors.lastname: Van Os
authors.forename: J A
authors.initials: JA
authors.lastname: McKinney
authors.forename: B J
authors.initials: BJ
authors.lastname: Zins
authors.forename: D C
authors.initials: DC
authors.lastname: Mays
authors.forename: Z H
authors.initials: ZH
authors.lastname: Schriver
authors.forename: W J
authors.initials: WJ
authors.lastname: Sandborn
authors.forename: J J
authors.initials: JJ
authors.lastname: Lipsky
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000964:Antimetabolites, Antineoplastic / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D018501:Antirheumatic Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D001379:Azathioprine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015122:Mercaptopurine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000964:Antimetabolites, Antineoplastic; D018501:Antirheumatic Agents; D007166:Immunosuppressive Agents; D015122:Mercaptopurine; D001379:Azathioprine