title: Visualization of antigen-specific T cell activation and cytokine expression in vivo.
abstract: A transgenic TCR adoptive transfer system was used to visualize Ag-specific T cell activation and cytokine expression in vivo. After s.c. injection of peptide in adjuvant the entire Ag-specific population up-regulated IL-2 receptor alpha-chain expression, underwent blast transformation, and developed a memory-surface phenotype. A minority of the Ag-specific T cells produced predominantly IL-2 mRNA and localized at the T cell/B cell junction in draining lymph nodes. In the secondary response, a mixed cytokine pattern of both Th1 and Th2 cytokines was demonstrated. When peptide was administered i.v. without adjuvant, 50% of the Ag-specific cells expressed IL-2, but the peak of expression occurred before IL-2 receptor alpha-chain up-regulation, and only a minority of the Ag-specific T cells underwent blast transformation.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, 35233, USA.
authors.forename: W O
authors.initials: WO
authors.lastname: Rogers
authors.forename: C T
authors.initials: CT
authors.lastname: Weaver
authors.forename: L A
authors.initials: LA
authors.lastname: Kraus
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: R P
authors.initials: RP
authors.lastname: Bucy
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004333:Drug Administration Routes; D005434:Flow Cytometry; D007114:Immunization / Q000379:methods; D017403:In Situ Hybridization; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008954:Models, Biological; D010047:Ovalbumin / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D010047:Ovalbumin