Evaluation of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose, a new fluorescent derivative of glucose, for viability assessment of yeast Candida albicans.
A new fluorescent derivative of D-glucose, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), which had been previously developed for the analysis of glucose uptake activity by living cells, was investigated to evaluate its applicability for assaying the viability of yeast Candida albicans. Lineweaver-Burk plots showed to uptake of 2-NBDG to be competitively inhibited by D-glucose and not by L-glucose, which suggested the involvement of the glucose transporting system of C. albicans in the uptake of 2-NBDG. A good correlation was obtained between the yeast viability, determined by the plate-count method, and the 2-NBDG uptake activity of yeast cells (correlation constant: r = 0.97). This is expected to lead to the development of a new fluorescent probe for the determination of yeast cell viability.
Yoshioka
K
K
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Oh
K B
KB
Saito
M
M
Nemoto
Y
Y
Matsuoka
H
H
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D001692:Biological Transport; D002176:Candida albicans / Q000166:cytology / Q000378:metabolism*; D003847:Deoxyglucose / Q000031:analogs & derivatives*; D000431:Ethanol / Q000494:pharmacology; D005456:Fluorescent Dyes; D005947:Glucose / Q000378:metabolism; D007700:Kinetics
D005456:Fluorescent Dyes; D000431:Ethanol; D003847:Deoxyglucose; D009327:4-Chloro-7-nitrobenzofurazan; D005947:Glucose; C098340:2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose