title: Relationship of the strain and the intraocular amount of herpes simplex virus types 1 and 2 in the induction of anterior-chamber-associated immune deviation.
abstract: We investigated the induction of anterior-chamber-associated immune deviation (ACAID) by herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Two strains of HSV-1 (VR-3 and F) and four strains of HSV-2 (UW-268, 186, SL, and MS) were inactivated with ultraviolet light, and the anterior chamber of BALB/c mice was inoculated with a viral strain. Immunization against delayed-type hypersensitivity (DTH) was done subcutaneously on day 7 after anterior chamber inoculation. On day 10 after DTH immunization, each virus was inoculated into the foot pad. DTH was evaluated by measuring the foot pad thickness at 24 h after the inoculation into the foot pad. DTH was suppressed by anterior chamber inoculation with more than 10(3) plaque-forming units (PFU) of the VR-3, UW-268, 186, and SL strains, and with 10(5) PFU of the F strain, but not by the MS strain. Until now, HSV-2 was believed not to induce ACAID, however, our results indicate that some HSV-2 strains induce ACAID.ACAID induction by HSV-1 and HSV-2 may depend on both the viral strain and the amount of ocular viral antigen.
authors.affiliation: Department of Microbiology, Asahikawa Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Akiba
authors.forename: I
authors.initials: I
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Suzutani
authors.forename: M
authors.initials: M
authors.lastname: Ogasawara
authors.forename: M
authors.initials: M
authors.lastname: Azuma
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology* / Q000821:virology; D002522:Chlorocebus aethiops; D005260:Female; D018259:Herpesvirus 1, Human / Q000502:physiology* / Q000528:radiation effects; D018258:Herpesvirus 2, Human / Q000502:physiology* / Q000528:radiation effects; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000517:prevention & control; D007114:Immunization; D016849:Keratitis, Herpetic / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D014466:Ultraviolet Rays; D014709:Vero Cells / Q000821:virology