title: From oligonucleotide shapes to genomic SELEX: novel biological regulatory loops.
abstract: The SELEX method and oligonucleotide combinatorial chemistry discovery process yields high-affinity/high-specificity ligands for virtually any molecular target. Typically, the enormous starting libraries used in the SELEX process contain 10(14)-10(15) sequences. We now ask if the smaller sequences, complexity of extant organisms, and evolutionary history provide useful interactions between oligonucleotides and at least some unexpected targets. That is, do organisms contain a robust "linkage map" between their oligonucleotides and proteins and/or small molecules that enriches life?
authors.affiliation: University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology, 80309-0347, USA.
authors.forename: L
authors.initials: L
authors.lastname: Gold
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: T
authors.initials: T
authors.lastname: Shtatland
authors.forename: B S
authors.initials: BS
authors.lastname: Singer
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004259:DNA-Directed DNA Polymerase; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D008024:Ligands; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D008024:Ligands; D009843:Oligoribonucleotides; D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D012313:RNA; D004259:DNA-Directed DNA Polymerase