Isolation and characterization of a bacterial growth-stimulating peptide from a peptic bovine hemoglobin hydrolysate.
A peptide with a bacterial-growth-stimulating activity was isolated from a bovine hemoglobin hydrolysate by reversed-phase high-performance liquid chromatography. Its primary structure and molecular mass, determined by amino acid analysis and fast-atom bombardment mass spectrometry, were identical to those of fragment 48-52 (Ser-Thr-Ala-Asp-Ala) of the beta chain of bovine hemoglobin. The microbiological tests in solid media demonstrated that this peptide exhibited a growth-stimulating activity on gram-negative bacteria.
Zhao
Q Y
QY
Laboratoire de Génie Protéique et Cellulaire, Université de La Rochelle, France.
Piot
J M
JM
Gautier
V
V
Cottenceau
G
G
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D006094:Gram-Positive Bacteria / Q000254:growth & development*; D006133:Growth Substances / Q000737:chemistry* / Q000302:isolation & purification*; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*
D000596:Amino Acids; D006133:Growth Substances; D006454:Hemoglobins; D010455:Peptides