Flagellar proteins of motile spores of Actinomycetes.
Flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by SDS-PAGE analysis to flagellins of cells of other bacteria. Several species of Actinoplanes have a major flagellar protein of subunit sizes of 42-43 kDa and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kDa. The flagellar protein sizes of other actinoplanetes ranged from 32-43 kDa (major) and 48-58 kDa (minor). Antibodies formed against the 42-kDa protein of A. rectilineatus showed cross-reactivity in Western blots against flagellar proteins of spores of other Actinoplanes species, two species of Dactylosporangium and an Ampullariella species. Cross-reactivity was also observed with motile cells of two other actinomycetes, Arthrobacter atrocyaneus and a Geodermatophilus species, and with Bacillus subtilis. No cross-reactivity was observed with Escherichia coli or Planomonospora parontospora flagellar proteins. The amino acid composition and partial N-terminal sequence of the 42-kDa flagellar protein of A. rectilineatus was compared to literature data for other bacterial flagellins and found to be most similar to B. subtilis 168.
Vesselinova
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NI
Department of Bacteriology, University of Wisconsin, Madison 53706, USA.
Ensign
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JC
D000192:Actinomycetales / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005407:Flagella / Q000737:chemistry* / Q000648:ultrastructure; D005408:Flagellin / Q000737:chemistry / Q000276:immunology; D008847:Micromonospora / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013170:Spores / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
D001426:Bacterial Proteins; D005408:Flagellin