title: Interaction with autologous platelets multiplies interleukin-1 and tumor necrosis factor production in mononuclear cells.
abstract: The effect of activated platelets on cytokine production by human peripheral blood mononuclear cells (PBMC) was investigated. When PBMC were coincubated with activated autologous platelets amid lipopolysaccharide (LPS, 50-100 pg/mL) for 8 h, the production of interleukin (IL)-1alpha increased 11- to 18-fold and tumor necrosis factor (TNF)-alpha 3- to 5-fold compared with PBMC without platelets. Activated platelets in a dual-chamber well that prevented platelet-PBMC contact but permitted passage of soluble factors enhanced IL-1alpha production (P < .01). Platelet-PBMC contact in the chamber resulted in a further enhancement of IL-1alpha production. These data suggest that platelet-PBMC interaction, both directly and with platelet-derived factors, enhances production of shock-producing IL-1alpha and TNF-alpha, albeit differently. The interaction of platelets with monocytes may play an important role in the pathophysiology of sepsis and disseminated intravascular coagulation.
authors.affiliation: Department of Medicine, Tufts University School of Medicine and New England Medical Center, Boston, MA 02111, USA.
authors.forename: K
authors.initials: K
authors.lastname: Aiura
authors.forename: B D
authors.initials: BD
authors.lastname: Clark
authors.forename: C A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: N H
authors.initials: NH
authors.lastname: Margolis
authors.forename: G
authors.initials: G
authors.lastname: Kaplanski
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
authors.forename: R G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: J A
authors.initials: JA
authors.lastname: Gelfand
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D015539:Platelet Activation; D013212:Staphylococcus epidermidis / Q000276:immunology; D013917:Thrombin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D013917:Thrombin