Follicular dendritic cells specifically express the long CR2/CD21 isoform.
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.
Liu
Y J
YJ
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Xu
J
J
de Bouteiller
O
O
Parham
C L
CL
Grouard
G
G
Djossou
O
O
de Saint-Vis
B
B
Lebecque
S
S
Banchereau
J
J
Moore
K W
KW
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002648:Child; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D003713:Dendritic Cells / Q000276:immunology*; D005091:Exons; D006801:Humans; D007739:L Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000276:immunology; D016133:Polymerase Chain Reaction; D017464:Receptors, Complement 3d / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D017931:DNA Primers; D018076:DNA, Complementary; D017464:Receptors, Complement 3d; D011994:Recombinant Proteins