title: A single vector cloning, mutagenesis and expression system.
abstract: We describe a multipurpose Escherichia coli vector, pOTSf1blue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authentic protein overexpression and efficient mutagenesis.
authors.affiliation: Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Towler
authors.forename: J W
authors.initials: JW
authors.lastname: Stebbins
authors.forename: C
authors.initials: C
authors.lastname: Debouck
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018741:Replication Origin / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded