Migration of IL-2-activated natural killer cells in vitro: influence of extracellular matrix proteins.
An experimental set-up for estimating a) cellular migration under agarose and b) response to chemoattractant gradients built up in the agarose was used in order to explore the behavior of adherent interleukin-2 (IL-2)-activated natural killer (A-NK) cells on cell culture plastic and after coating with extracellular matrix (ECM) constituents. A-NK cells were deposited in wells in the agarose and directed migration, chemotaxis, towards aggregates and suspensions of B16F10 melanoma cells, suspensions of YAC-1 cells, and tumor-conditioned media, all deposited in wells at a 2.5 mm distance, was tested. A-NK cell chemotaxis was exclusively observed when B16F10 aggregates were used as attractants. The substrate influenced chemotaxis considerably, untreated plastic surface being most favorable for a chemotactic response, followed by laminin, fibronectin, and collagen IV pretreatments. Coating with reconstituted basement membrane matrix (Matrigel) gave lesser random movements, chemokinesis, of A-NK cells than coating with the purified components laminin and collagen IV, and the least motile response was obtained after collagen I pretreatment. These in vitro observations indicate that melanoma cell aggregates release humoral factors of a probably short-lived nature with a chemoattractant effect on A-NK cells, and that ECM composition influences migratory response, both conclusions with a bearing on the understanding of A-NK cell infiltration into tumors in vivo.
Unger
M L
ML
Institute of Anatomy and Cell Biology, University of Göteborg, Sweden.
Johansson
B R
BR
Nannmark
U
U
D000818:Animals; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D016326:Extracellular Matrix Proteins / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000166:cytology*; D008213:Lymphocyte Activation; D008546:Melanoma, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D014407:Tumor Cells, Cultured
D016326:Extracellular Matrix Proteins; D007376:Interleukin-2