Frequencies of X-ray induced pericentric inversions and centric rings in human blood lymphocytes detected by FISH using chromosome arm specific DNA libraries.
Frequencies of intra-chromosomal exchanges (pericentric inversions and centric rings) and inter-chromosomal exchanges (dicentrics and translocations) in X-irradiated (2.5 Gy) human lymphocytes have been estimated. To detect these events we employed FISH (fluorescence in situ hybridization) technique and arm specific painting probes for chromosomes #1 and #3. The ratio between centric rings and pericentric inversions was found to be about 1. For intra-changes to inter-changes, the ratio (F) was between 6 and 9. Based on the total number of colour junctions involving chromosomes #1 and #3 it was found that exchanges between the arms of the same chromosome occur about 8.7 times more than inter-chromosomal exchanges calculated on the basis of the DNA content of the chromosomes and random induction of aberrations in the total genome. Chromosomal organization in interphase nucleus appears to promote the formation of more intra-changes than inter-changes following X-irradiation, most probably due to close proximity of the two arms of a chromosome.
Natarajan
A T
AT
Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, Netherlands. Natarajan@rullf2.medfac.leidenuniv.nl
Boei
J J
JJ
Vermeulen
S
S
Balajee
A S
AS
D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D002877:Chromosomes, Human / Q000528:radiation effects*; D002878:Chromosomes, Human, Pair 1 / Q000528:radiation effects; D002893:Chromosomes, Human, Pair 3 / Q000528:radiation effects; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic; D014965:X-Rays