Analysis of kafirin promoter activity in transgenic tobacco seeds.
Sequences corresponding to 855 bp of 5' promoter region and the transit peptide from lambdaGK.1,a genomic clone encoding a 22 kDa alpha-kafirin seed protein from sorghum, were translationally fused to a cloned beta-glucuronidase (GUS) coding sequence from uidA and transferred to tobacco via Agrobacterium tumefaciens-mediated transformation. No GUS expression was detectable at any stage of growth in stems or leaves of these plants. However, GUS expression was detected in both embryo and endosperm tissues of resulting tobacco seeds 10-15 days after flowering. Dissected tissues indicate endosperm expression was localized within the bulk endosperm and not within the parenchyma cell layer underlying the integument. These studies also demonstrate that within dissected tobacco embryos, expression from the kafirin promoter was restricted to the mesocotyl region.
DeRose
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Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, College Station 77843-3155, USA.
Begum
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Hall
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D003001:Cloning, Molecular; D002523:Edible Grain / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012639:Seeds / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic; D015028:Zein / Q000235:genetics
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C080615:kafirin protein, Sorghum bicolor; D015028:Zein; D005966:Glucuronidase