Signaling effects of alpha-thrombin and SFLLRN in rat glioma C6 cells.
Effects of thrombin on brain cells, including change of neurite outgrowth and astrocyte shape, are described, but the molecular mechanisms are unclear. We investigated the effects of human alpha-thrombin and a six amino acid thrombin receptor activating peptide (TRAP-6, SFLLRN) on [Ca2+]i, phosphoinositide hydrolysis, and protein kinase C in rat glioma C6 cells. Stimulation of C6 cells with both alpha-thrombin and TRAP-6 resulted in [Ca2+]i mobilization, [3H]Inositol phosphate response, and enhanced immunoreactivity of the protein kinase C (PKC) isoenzymes alpha, beta, gamma, delta, and epsilon. Results suggest that alpha-thrombin and TRAP-6 activate at least partially the same intracellular signaling pathways in rat glioma C6 cells, which is evidence for involvement of "tethered ligand" receptor in thrombin induced signaling in glioma C6 cells.
Kaufmann
R
R
Max Planck Gesellschaft, Research Unit, Pharmacological Hemostaseology, Friedrich Schiller University Jena, Germany.
Lindschau
C
C
Höer
A
A
Henklein
P
P
Adomeit
A
A
Haller
H
H
Liebmann
C
C
Oberdisse
E
E
Nowak
G
G
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004789:Enzyme Activation; D005456:Fluorescent Dyes; D005910:Glioma; D006801:Humans; D007295:Inositol Phosphates / Q000493:pharmacokinetics; D007527:Isoenzymes / Q000378:metabolism; D018613:Microscopy, Confocal; D010446:Peptide Fragments / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D051381:Rats; D018179:Receptors, Thrombin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D007527:Isoenzymes; D010446:Peptide Fragments; D018179:Receptors, Thrombin; C082835:thrombin receptor peptide (42-47); D014316:Tritium; C497260:Prkcd protein, rat; C497257:Prkce protein, rat; C087857:protein kinase C gamma; C496628:PRKCA protein, human; C497258:PRKCD protein, human; C497255:PRKCE protein, human; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D013917:Thrombin; D002118:Calcium