title: In vitro survey of alpha-glucosidase inhibitory food components.
abstract: A survey of food components with alpha-glucosidase (AGH) inhibitory activity was conducted to identify a prophylactic effect for diabetes in food. Sardine muscle hydrolyzed by alkaline protease showed potent activity (IC50 = 48.7 mg/ml) as well as green and oolong teas (IC50 = 11.1 and 11.3 mg/ml, respectively). Furthermore, hydrolyzates prepared by various proteases gave differing AGH inhibitory activity. DEAE-Sephadex chromatography of the alkaline protease hydrolyzate eluted potent AGH inhibitors (IC50 = 15.6 mg/ml) with a 50 mM phosphate buffer (pH 7.0) containing 0.3 M NaCl, and their subsequent separation by HPLC in an ODS column showed that there were some inhibitors possessing primary amino groups. This indicates that they would have been high anionic and peptidic compounds.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: C
authors.initials: C
authors.lastname: Yoshimoto
authors.forename: K
authors.initials: K
authors.lastname: Osajima
authors.forename: T
authors.initials: T
authors.lastname: Oki
authors.forename: Y
authors.initials: Y
authors.lastname: Osajima
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D005399:Fishes; D005504:Food Analysis; D065089:Glycoside Hydrolase Inhibitors; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D011492:Protein Hydrolysates / Q000032:analysis / Q000494:pharmacology; D013662:Tea / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D011492:Protein Hydrolysates; D013662:Tea