The effect of neuropeptides on vessel tone and cAMP production.
The effect of VIP, PHM, PHV, PACAP-27, and PACAP-38 on vessel tone and cAMP production was investigated in rabbit ovarian arteries in vitro. The peptides (10(-7)M) induced a significant relaxation on NA-precontracted vessels and displayed similar potencies. The cAMP accumulation induced by PACAP-27 and PACAP-38 was five times higher than the cAMP content induced by VIP, PHM, and PHV. NPY (10(-7)M) markedly reversed the relaxations induced by VIP, PHM, PHV, PACAP-27, and PACAP-38 but did not at all affect the cAMP production induced by these peptides. We conclude that the relaxation induced by VIP, PHM, PHV, PACAP-27, and PACAP-38 and the contraction evoked by NPY are not solely related to the changes of cAMP contents, and that in addition to cAMP, another intracellular signal transduction pathway may be involved.
Yao
W
W
Department of Obstetrics and Gynecology, Hvidovre Hospital, University of Copenhagen, Denmark.
Sheikh
S P
SP
Ottesen
B
B
Jørgensen
J C
JC
D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects; D009129:Muscle Tonus / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010053:Ovary / Q000098:blood supply*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D009638:Norepinephrine