title: Complete recovery by nerve growth factor of neuropeptide content and function in capsaicin-impaired sensory neurons.
abstract: In the present study the ability of nerve growth factor (NGF) to facilitate the recovery of peptidergic primary sensory C-fibers after an acute capsaicin treatment (50 mg/kg s.c.) was investigated in adult rats. NGF (4 micrograms 1/day for 3 days) was injected into the plantar of one hind paw starting 24 h after the capsaicin treatment. Without NGF, there was a significant reduction of calcitonin gene-related peptide (CGRP) and substance P content of the paw skin and the sciatic nerve. CGRP and substance P levels were completely replenished in the NGF-treated paw skin and in the innervating sciatic nerve they even increased over control levels as determined 40 h after the last injection of NGF. CGRP levels also recovered in the contralateral paw and sciatic nerve, but no recovery was observed in other tissues such as the front paw, the auricle, or the urinary bladder. Mustard oil-induced neurogenic plasma extravasation, taken as a functional parameter for peptidergic primary sensory C-fibers, was significantly decreased after the capsaicin treatment and showed a complete recovery by NGF in the injected paw as well as in the contralateral paw skin. These results show that NGF not only was able to reverse the decrease of transmitter content caused by capsaicin but also restored the peripheral function of primary afferent neurons.
authors.affiliation: Department of Experimental and Clinical Pharmacology, University of Graz, Austria. josef.donnerer@kfunigraz.ac.at
authors.forename: J
authors.initials: J
authors.lastname: Donnerer
authors.forename: R
authors.initials: R
authors.lastname: Amann
authors.forename: R
authors.initials: R
authors.lastname: Schuligoi
authors.forename: G
authors.initials: G
authors.lastname: Skofitsch
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002211:Capsaicin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000187:drug effects / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects
chemical_list: D001798:Blood Proteins; D009414:Nerve Growth Factors; D009479:Neuropeptides; D002211:Capsaicin