title: [Use of microtest systems for identification of newly isolated clinical strains].
abstract: Species appurtenance of 425 clinical strains isolated from various types of material from December 1993 to November 1994 was identified using Lachema (Czechia) Micro-la-test. There were 99 staphylococcal, 139 streptococcal, 119 enterobacterial cultures, and 68 nonfermenting gram-negative bacteria. The microorganisms were characterized completely according to their code in 67.7% cases (STAPHYtest), 54% (STREPTOtest), 25.2% (ENTEROtest), and 22.1% (NEFERMtest). False-positive reactions in the control were detected in 22.2% (STAPHYtest), 33.3% (STREPTOtest), 11.8% (ENTEROtest), and 41.7% (NEFERMtest) of the number of biochemical characteristics in a plaque. Unclear reactions (+/-) making identification difficult were observed in 100% cases for STAPHYtest, 83.3% for STREPTOtest, 91.7% for ENTEROtest-1, and 58.3% for NEFERMtest. Atypical reactions, not corresponding to the studied species, were observed in 62.5% cases with STAPHYtest, 41.7% with STREPTOtest, 66.7% with ENTEROtest-1, and 58.3% with NEFERMtest. Still, STREPTOtest with the probability of verifying 61.5 to 87.5% of the cultures may be used for identifying the species appurtenance of alpha-hemolytic streptococci.
authors.forename: K I
authors.initials: KI
authors.lastname: Savitskaia
authors.forename: T V
authors.initials: TV
authors.lastname: Chernen'kaia
authors.forename: T A
authors.initials: TA
authors.lastname: Silkina
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006801:Humans; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification