Flow cytometry evaluation of complement mediated bacterial membrane damage.
The study of the complement-outer bacterial membrane interactions is gaining enthusiasm for applying a continuous development of more specialized techniques. In this paper a novel flow cytometry technique shows that highly fluorescent lucifer yellow-stained Neisseriae gonorrhoeae when exposed to the redox reaction of p-nitro blue chloride tetrazolium (NBT), as formazan precipitation takes place, a shift to lesser fluorescent channels of the histogram population occurs. That effect is labeled NBT-laser beam quenching (NBT-LBQ). A significant difference by Kolmogorov-Smirnov summation curve analysis is found between complement heat-inactivated and its counterpart assessed with normal complement microorganisms. The operation of this NBT-LBQ effect by microbial flow cytometry casts an interesting potential for the evaluation of the outer membrane-complement-interaction of serum sensitive microorganisms.
Alvarado-Alemán
F J
FJ
Departamento de Medicina Geográfica, Hospital General Dr. Jesús Kumate, Cancún, Q. Roo, México.
D002462:Cell Membrane / Q000187:drug effects* / Q000473:pathology; D003165:Complement System Proteins / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007546:Isoquinolines / Q000737:chemistry; D009344:Neisseria gonorrhoeae / Q000737:chemistry*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D013194:Staining and Labeling
D007546:Isoquinolines; D009580:Nitroblue Tetrazolium; D003165:Complement System Proteins; C017475:lucifer yellow