title: Arbitrary primer PCR of dog DNA with estimates of average heterozygosity.
abstract: Arbitrary primer polymerase chain reaction (AP-PCR) detected anonymous DNA polymorphisms between individual representatives of seven breeds of dogs, between members of a single breed, and between members of a single family of dogs. Short arbitrary sequence PCR primers and promoter/protein motif sequence primers typically amplified 15-30 bands ranging from 200-2000 bp in length. The average heterozygosity of 10 line bred Belgian sheepdogs (H = 0.024 +/- 0.011, mean +/- 95% CI) was significantly lower than that of a panel of unrelated beagles (H = 0.057 +/- 0.018, mean +/- 95% CI, P < .01). The average heterozygosity of a seven-breed panel was intermediate between the two (H = 0.043 +/- 0.011, mean +/- 95% CI) and not significantly different than either panel. One motif sequence PCR primer pair yielded products that strongly differentiated between Belgian sheepdogs, beagles, and members of five other breeds. AP-PCR could be used to generate DNA fingerprints that distinguish between dog breeds, as well as to provide markers in this sparsely mapped genome.
authors.affiliation: Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38163, USA.
authors.forename: A D
authors.initials: AD
authors.lastname: Ezer
authors.forename: R W
authors.initials: RW
authors.lastname: Williams
authors.forename: D
authors.initials: D
authors.lastname: Goldowitz
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D006580:Genetic Carrier Screening; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D011506:Proteins; D004247:DNA