title: Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures: mimicry of arterial oxygen pressures by H2O2.
abstract: Liver glucokinase (GK) is localized predominantly in the perivenous zone. GK mRNA was induced by insulin maximally under venous O2 partial pressure (pO2) and only half-maximally under arterial pO2. CoCl2 and desferrioxamine mimicked venous pO2 and enhanced the insulin-dependent induction of GK mRNA under arterial pO2. H2O2 mimicked arterial pO2 and reduced insulin-induced GK mRNA under venous pO2 to the lower arterial levels. Thus the zonal O2 gradient in liver seems to have a key role in the heterogenous expression of the GK gene.
authors.affiliation: Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Kietzmann
authors.forename: U
authors.initials: U
authors.lastname: Roth
authors.forename: S
authors.initials: S
authors.lastname: Freimann
authors.forename: K
authors.initials: K
authors.lastname: Jungermann
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D001785:Blood Gas Monitoring, Transcutaneous; D003035:Cobalt; D003676:Deferoxamine / Q000494:pharmacology; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005941:Glucokinase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016587:Antimutagenic Agents; D007328:Insulin; D012333:RNA, Messenger; D003035:Cobalt; D006861:Hydrogen Peroxide; D005941:Glucokinase; C018021:cobaltous chloride; D003676:Deferoxamine