New HLA-DR haplotypes containing the DRB6 pseudogene.
An unexpected probe reaction pattern was observed in two samples during HLA-DR typing by PCR-Sequence Specific Oligonucleotide Probes. In order to confirm the unusual typings, samples were analyzed by PCR-Sequence Specific Primers, cloning, and nucleotide sequencing of the second exon of the HLA-DRB-genes. The confirmed DR, DQ phenotype for one sample was DRB1*0701, DRB4*01, DRB5*0101, DRB6*0201, DQB*0602, DQB1*0202. The phenotype of other sample was DRB1*1602, DRB1*1302, DRB3*0301, DRB6*0101, DQB1*0501, DQB1*0502. The first sample has the novel combination of DRB1*0701 with DRB5*0101 and DRB6*0201. The second sample has either DRB6*0101 together with DRB1*1602 in absence of any DRB5 allele or DRB6*0101 together with DRB1*1302, DRB3*0301. We postulate that the most likely haplotype in sample #1 is DRB1*0701; DRB5*0101, DRB1*0602 which could have arisen from gene conversion. The most likely haplotype in sample #2, DRB1*1602, DRB6*0101, DQB1*0502 would have arisen from an homologous recombination event.
Salazar
M
M
American Red Cross, New England Region, Dedham, Massachusetts, USA.
Granja
C B
CB
Selvakumar
A
A
Fresia
B
B
Alosco
S
S
Dupont
B
B
Yunis
E J
EJ
D006684:HLA-DR Antigens / Q000235:genetics*; D059806:HLA-DR beta-Chains; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction; D011544:Pseudogenes
D006684:HLA-DR Antigens; D059806:HLA-DR beta-Chains; C069699:HLA-DRB6 antigen