Identification and expression of a variant isoform of the levocabastine-sensitive neurotensin receptor in the mouse central nervous system.
This work describes the molecular cloning of a variant isoform of the low-affinity levocabastine-sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS-7 cells, this non-functional neurotensin receptor is expressed in cerebral neocortex, cerebellum, olfactory bulb, striatum and hypothalamus with a level similar to that of the full-length low-affinity neurotensin receptor. By contrast, this receptor form is very weakly expressed in mesencephalon and absent in the pituitary, but is the major product in the spinal cord.
Botto
J M
JM
Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, UPR 411, Valbonne, France.
Sarret
P
P
Vincent
J P
JP
Mazella
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D009496:Neurotensin / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology*; D018028:Receptors, Neurotensin / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D010880:Piperidines; D018028:Receptors, Neurotensin; D009496:Neurotensin; C047340:levocabastine