title: Instability of chromosome 6 microsatellite repeats in human cervical tumors carrying papillomavirus sequences.
abstract: Loss of heterozygosity (LOH) in chromosome 6 in human squamous cervical carcinomas was analyzed in the long and short arms of the chromosome using 3 pairs of primers each. In all cases, normal adjacent tissue was used as control. Among 51 cases analyzed, we identified LOH or microsatellite instability in 23% using primer D6S291 (located at position 6p21.3) and in 11% using primers D6S308 (6q16.3-6q27) and D6S270 (6q22.3-6q23.2). On the contrary, no significant LOH or genomic instabilities were detected with primers D6S306 (6p22.3-6p21.2), D6S299 (6p22.3-6p21.3) and D6S287 (6q21-6q23.3). Our results thus suggest the existence of instable loci at 3 regions of chromosome 6. Whether these loci contain putative tumor-suppressor genes or genes involved in cell cycle control remains unknown.
authors.affiliation: Department of Viral Molecular Biology, Kazan Cancer Research Center, Moscow, Russia.
authors.forename: F
authors.initials: F
authors.lastname: Kisseljov
authors.forename: L
authors.initials: L
authors.lastname: Semionova
authors.forename: E
authors.initials: E
authors.lastname: Samoylova
authors.forename: N
authors.initials: N
authors.lastname: Mazurenko
authors.forename: E
authors.initials: E
authors.lastname: Komissarova
authors.forename: V
authors.initials: V
authors.lastname: Zourbitskaya
authors.forename: T
authors.initials: T
authors.lastname: Gritzko
authors.forename: V
authors.initials: V
authors.lastname: Kozachenko
authors.forename: M
authors.initials: M
authors.lastname: Netchushkin
authors.forename: S
authors.initials: S
authors.lastname: Petrov
authors.forename: A
authors.initials: A
authors.lastname: Smirnov
authors.forename: A
authors.initials: A
authors.lastname: Alonso
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005260:Female; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014412:Tumor Virus Infections / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D004273:DNA, Neoplasm