[Comparative evaluation of test-systems for detection of Venezuelan equine encephalomyelitis virus and variola virus using dot immunoenzyme assay and solid-phase lanthanide immunofluorescent assay].
The sensitivity and specificity of 4 experimental test systems for dot enzyme immunoassay (dot-EIA) and solid-phase lanthanide immunofluorescent analysis (SP LIFA) were studied with Venezuelan equine encephalomyelitis (VEE) and variolovaccinia viruses. Test systems for SP LIFA proved to be 25 times more sensitive than those for dot-EIA. The test systems were highly specific and did not react with the heterologous viruses and proteins. Diagnostic agent for detecting VEE virus in dot-EIA was false-positive with Sindbis virus in low dilutions. The first trials of the Russian test systems for dot-EIA and SP LIFA showed that these systems rapidly and reliably detect VEE and variolovaccinia viruses in liquid samples.
Gradoboev
V N
VN
Pomelova
V G
VG
Bychenkova
T A
TA
Moskvina
T M
TM
Lavrova
N A
NA
Markov
V I
VI
Osin
N S
NS
Butenko
A M
AM
D000956:Antigens, Viral / Q000032:analysis; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008674:Metals, Rare Earth; D014616:Vaccinia virus / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D008674:Metals, Rare Earth