title: Affinity purification of cellulose-binding enzymes of Clostridium stercorarium.
abstract: Cellulose-affinity chromatography proved to be a fast and efficient purification procedure for exoenzymes of the thermophilic anaerobe Clostridium stercorarium, suitable for the preparation of large amounts of enzymes for technical applications. The cellulose-binding enzymes could be identified as the C. stercorarium; cellulolytic enzymes Avicelase I and Avicelase II characterized previously as endo-1,4-beta-glucanase and exo-1,4-beta-glucanase. A third protein was identified as xylanase A, the major endo-1,4-beta-xylanase of this organism.
authors.affiliation: Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Bronnenmeier
authors.forename: H
authors.initials: H
authors.lastname: Adelsberger
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: W L
authors.initials: WL
authors.lastname: Staudenbauer
mesh_terms: D000595:Amino Acid Sequence; D001709:Biotechnology; D002480:Cellulase / Q000235:genetics / Q000302:isolation & purification; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D043364:Endo-1,4-beta Xylanases; D044643:Glucan 1,4-beta-Glucosidase; D008969:Molecular Sequence Data; D014995:Xylosidases / Q000235:genetics / Q000302:isolation & purification; D001617:beta-Glucosidase / Q000302:isolation & purification
chemical_list: D002482:Cellulose; D014995:Xylosidases; D001617:beta-Glucosidase; D002480:Cellulase; D044643:Glucan 1,4-beta-Glucosidase; D043364:Endo-1,4-beta Xylanases; D043366:Cellulose 1,4-beta-Cellobiosidase