title: Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats.
abstract: We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of mossy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of mossy fibres.
authors.affiliation: Laboratory of Neuropharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bendotti
authors.forename: M
authors.initials: M
authors.lastname: Pende
authors.forename: F
authors.initials: F
authors.lastname: Guglielmetti
authors.forename: R
authors.initials: R
authors.lastname: Samanin
mesh_terms: D000818:Animals; D001345:Autoradiography; D003513:Cycloheximide / Q000494:pharmacology*; D018891:Dentate Gyrus / Q000187:drug effects* / Q000378:metabolism; D019922:GAP-43 Protein; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D003513:Cycloheximide; D007608:Kainic Acid