Substantially complete removal of the 34kDa allergenic soybean protein, Gly m Bd 30 K, from soy milk of a mutant lacking the alpha- and alpha'-subunits of conglycinin.
Substantially complete removal (99.78%) of the allergenic soybean protein, Gly m Bd 30 K, was attained by applying simple centrifugation at 10,000 x g and at pH 4.5 in the presence of Na2SO4 to defatted soy milk from a genetically mutated soybean cultivar (Tohoku 124) which lacked the alpha- and alpha'-subunits of conglycinin. The removal rate was ten times higher than that (97.44%) from a normal soybean cultivar of I.O.M. grade.
Samoto
M
M
Central Research Institute, Fuji Oil Co., Ltd, Ibaraki, Japan.
Takahashi
K
K
Fukuda
Y
Y
Nakamura
S
S
Kawamura
Y
Y
D000485:Allergens / Q000235:genetics* / Q000302:isolation & purification*; D052179:Antigens, Plant; D015152:Blotting, Northern; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification*; D012972:Sodium Hydroxide; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics*
D000485:Allergens; D052179:Antigens, Plant; C103236:Gly m BD 30K protein, Glycine max; D010940:Plant Proteins; D030262:Soybean Proteins; D012972:Sodium Hydroxide