High-performance liquid chromatography/NMR spectrometry/mass spectrometry: further advances in hyphenated technology.
The earlier use of combined liquid chromatography/NMR spectrometry/mass spectrometry (LC/NMR/MS) involved the use of a particle beam interface. This paper describes further developments of this hyphenated technology, in particular the incorporation of an electrospray interface into the LC/NMR/MS system. This improved LC/NMR/MS system was designed for the support of a combinatorial library program. The power of this technique is demonstrated in the successful structural elucidation of each compound in a mixture of commercially available peptides.
Holt
R M
RM
Pfizer Central Research, Sandwich, Kent, UK.
Newman
M J
MJ
Pullen
F S
FS
Richards
D S
DS
Swanson
A G
AG
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D013053:Spectrophotometry
D010455:Peptides