The cloning, sequence analysis, and expression in E. coli of coat protein gene of grapevine fanleaf virus Gh.
Fragments of 5' end and 3' end of the coat protein gene of a Chinese strain grapevine fanleaf virus (GFLV-Gh) were separately amplified through RT-PCR. The two products were cloned and ligated into a complete full-length coat protein gene via an inherent BgIII restriction site. The entire gene contains 1512 nucleotides which encode a 504 amino acids coat protein, molecular weight approximately 56 kDa. Comparison with GFLV F-13 shows 88.4% homology in nucleotides and 95.8% similarity in deduced amino acid residues. Also this coat protein gene was expressed in E. coli DH 5 alpha.
Guan
H
H
Department of Virology, Chinese Academy of Sciences, Beijing, China.
Cai
W
W
Mang
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005638:Fruit / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D011994:Recombinant Proteins