title: Structure and function studies of factor XIIIa by x-ray crystallography.
abstract: The three-dimensional structures of several forms of the factor XIII A subunit have been determined using single crystal x-ray diffraction methods. Our crystallographic studies have provided the first detailed structural view of the factor XIII A subunit and information that is useful for understanding transglutaminase function. We have identified a conserved Cys314-His373-Asp396 catalytic triad of residues in the active site of the molecule and a number of other conserved residues that may play important roles as well. The calcium and strontium structures have revealed several conserved acidic residues (Asp438, Glu485, and Glu490) involved in ion binding. We have also been able to use our crystal structures as scaffolds to model the possible structural effects of missense mutations that have been identified in factor XIII-deficient patients.
authors.affiliation: Biochemistry Department, University of Washington, Seattle 98195-7350, USA.
authors.forename: V C
authors.initials: VC
authors.lastname: Yee
authors.forename: I
authors.initials: I
authors.lastname: Le Trong
authors.forename: P D
authors.initials: PD
authors.lastname: Bishop
authors.forename: L C
authors.initials: LC
authors.lastname: Pedersen
authors.forename: R E
authors.initials: RE
authors.lastname: Stenkamp
authors.forename: D C
authors.initials: DC
authors.lastname: Teller
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000737:chemistry / Q000502:physiology; D018360:Crystallography, X-Ray; D003546:Cysteine Endopeptidases / Q000737:chemistry; D019281:Dimerization; D004792:Enzyme Precursors / Q000737:chemistry; D005177:Factor XIII Deficiency / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D017354:Point Mutation; D011487:Protein Conformation; D013324:Strontium / Q000737:chemistry; D013329:Structure-Activity Relationship; D013917:Thrombin / Q000378:metabolism; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
chemical_list: D004792:Enzyme Precursors; D011503:Transglutaminases; D013917:Thrombin; D003546:Cysteine Endopeptidases; D002118:Calcium; D013324:Strontium