Effect of administered ethanol on protein kinase C in human platelets.
There are numerous reports of the effect of ethanol on protein kinase C (PKC) in animals or with in vitro systems. However, the effect of ethanol on PKC in humans has not been extensively investigated despite the large number of studies involving PKC and human platelets. In this study, we administered ethanol to human volunteers and determined the level of PKC before and after a 0.4 g/kg dose of ethanol. We studied Native Americans and Caucasians of both sexes. There was an increases in PKC activity 60 min after ethanol administration. There were no ethnic, age, nor gender differences detected, nor was there any correlation between family history of alcoholism and the basal or stimulated platelet PKC levels. Neither was there any correlation of basal or stimulated PKC activity with the genotypes for ADH2, ADH3, ALDH2, CYP2E1, and CYP1A2.
Deitrich
R A
RA
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
Bludeau
P
P
Elk
M E
ME
Baker
R
R
Menez
J F
JF
Gill
K
K
D000328:Adult; D000367:Age Factors; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D019388:Cytochrome P-450 CYP1A2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005838:Genotype; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D007262:Infusions, Intravenous; D008297:Male; D008875:Middle Aged; D011493:Protein Kinase C / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors; D013268:Stimulation, Chemical; D044465:White People / Q000235:genetics
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D019388:Cytochrome P-450 CYP1A2; D000444:Aldehyde Dehydrogenase; D011493:Protein Kinase C