title: Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview.
abstract: This paper presents a synthesis of 3 year results of in vitro production of bovine embryos in medium previously conditioned by bovine oviduct epithelial cells. In Louvain-la-Neuve, Belgium, a total of 18356 oocytes were matured and inseminated in vitro: 13967 (76%) had cleaved at 3 days post-insemination and 3593 (26%) became blastocysts using this culture system. Our data show that conditioned medium can be stored frozen for up to 3 years without significant loss of activity and is resistant to lyophilization. One single batch of conditioned medium was tested within the same period in four different laboratories and yielded variable results: 27 and 37% blastocysts/cleaved embryos in two of them and only 7 and 0% in the two others whereas in each case more than 30% blastocysts were obtained with the local reference co-culture system. In one laboratory, the batch of oil used to overlay the culture drops had a detrimental effect on the blastocyst rate in conditioned medium but not in co-culture.
authors.affiliation: Unité des sciences vétérinaires, université catholique de Louvain, Louvain-La-Neuve, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Van Langendonckt
authors.forename: A
authors.initials: A
authors.lastname: Vansteenbrugge
authors.forename: I
authors.initials: I
authors.lastname: Donnay
authors.forename: A
authors.initials: A
authors.lastname: Van Soom
authors.forename: U
authors.initials: U
authors.lastname: Berg
authors.forename: E
authors.initials: E
authors.lastname: Semple
authors.forename: B
authors.initials: B
authors.lastname: Grisart
authors.forename: P
authors.initials: P
authors.lastname: Mermillod
authors.forename: G
authors.initials: G
authors.lastname: Brem
authors.forename: A
authors.initials: A
authors.lastname: Massip
authors.forename: F
authors.initials: F
authors.lastname: Dessy
mesh_terms: D000818:Animals; D002417:Cattle; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005612:Freeze Drying; D005615:Freezing; D011247:Pregnancy
chemical_list: D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free