Isolation and characterization of the bioactive circulating human parathyroid hormone, hPTH-1-37.
The occurrence of hPTH-1-37 as the native bioactive circulating form of PTH-1-84 has now been obtained using a specific purification procedure for circulating parathyroid hormone, which involves a newly developed immunoenzymetric assay for N-terminally intact hPTH. In combination with two different methods of mass spectrometry, the molecular weight of the isolated immunoreactive peptide was shown to be 4401 Da, which corresponds to hPTH-1-37. Synthetic hPTH-1-37 material was tested in the chick bioassay and produced a clearcut increase in serum calcium concentration. We conclude that hPTH-1-37 is the native bioactive fragment of hPTH-1-84 in circulation.
Hock
D
D
Niedersächsisches Institut für Peptid-Forschung (IPF), Hannover, Germany. Hock@novsrv1.pio1.uni-Heidelberg.de
Mägerlein
M
M
Heine
G
G
Ochlich
P P
PP
Forssmann
W G
WG
D000818:Animals; D002645:Chickens; D006801:Humans; D010281:Parathyroid Hormone / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D010281:Parathyroid Hormone; D010446:Peptide Fragments; C094316:parathyroid hormone (1-37)