Review: preimplantation diagnosis of inherited disease.
Preimplantation diagnosis of inherited diseases has become possible with the techniques of in vitro fertilization, blastomere biopsy of the 6- to 10-cell embryo and DNA analysis of the single blastomeres. Disease-free embryos are selected for transfer to the uterus, thereby avoiding the need for termination of a fetus diagnosed as affected in prenatal diagnosis in the first or early-second trimester of pregnancy. The genetic indications for preimplantation diagnosis are theoretically the same as for prenatal diagnosis, but the defects must be detectable by the polymerase chain reaction. For X-linked recessive diseases, fluorescence in situ hybridization can be used as an alternative for the selection of female embryos. So far almost 40 healthy children have been born worldwide after preimplantation diagnosis for genetic disease. The possibilities and limitations of preimplantation diagnosis, especially in prevention of inherited disease, are discussed in this review.
Lissens
W
W
Centre for Medical Genetics, University Hospital of the Dutch-speaking Brussels Free University (Vrije Universiteit Brussel, Belgium.
Sermon
K
K
Staessen
C
C
Assche
E V
EV
Janssenswillen
C
C
Joris
H
H
Van Steirteghem
A
A
Liebaers
I
I
D000483:Alleles; D001757:Blastomeres / Q000737:chemistry* / Q000473:pathology; D004247:DNA / Q000032:analysis*; D005260:Female; D005307:Fertilization in Vitro; D008040:Genetic Linkage; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis; D014960:X Chromosome
D004247:DNA