The C-terminal Src kinase (Csk) is widely expressed, active in HT-29 cells that contain activated Src, and its expression is downregulated in butyrate-treated SW620 cells.
Csk expression was examined in human glioma, breast and colon cell lines, along with murine tissues. Relative to colon and glioma cell lines, Csk expression was highest in the breast cell lines. Relative to brain tissue, expression was 100-fold higher in the heart, kidney, liver, lung and spleen. Subcellular fractionation and cellular immunostaining indicated that it was localized in the cytosolic fraction. The expression of Csk was downregulated upon chemical-induced differentiation of SW620 human colon cells by treatment with sodium butyrate. Its expression localization and activity relative to Src are discussed.
Li
S
S
Department of Neuro-Oncology, M.D. Anderson Cancer Center, Houston, TX 77030, USA.
Ke
S
S
Budde
R J
RJ
D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D000081247:CSK Tyrosine-Protein Kinase; D002454:Cell Differentiation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology; D004790:Enzyme Induction / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D019061:src-Family Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D002087:Butyrates; D009363:Neoplasm Proteins; D020148:Butyric Acid; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases; C000631346:CSK protein, human