Variation in Salmonella core lipopolysaccharide as detected by the monoclonal antibody M105.
The lipopolysaccharide antigenicity of 22 Salmonella strains (representing nine serogroups) and four non-salmonellae Enterobacteriaceae to the Salmonella genus specific monoclonal antibody M105 was analysed. The monoclonal antibody M105 reacted with all 22 Salmonella strains. Probing SDS-PAGE separated LPS molecules with MAb M105 revealed that the antibody reacted with the core region of all Salmonella serovars. However, no reaction was obtained to the long-chain LPS of serovars O (Salm. adelaide and Salm. ealing), C1 (Salm. infantis, Salm. livingstone and Salm. virchow) or Salm. arizonae. It is plausible that the presence of a second core antigenic type results in the lack of reaction between long-chain LPS and the Salmonella genus specific monoclonal antibody M105.
Mansfield
L P
LP
Department of Life Sciences, Nottingham Trent University, UK.
Billett
E
E
Olsen
E
E
Forsythe
S J
SJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D016971:Citrobacter freundii / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004754:Enterobacter / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology*; D012475:Salmonella / Q000276:immunology*; D012763:Shigella flexneri / Q000276:immunology
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides