Antimutagenic effects of ajoene, an organosulfur compound derived from garlic.
The antimutagenic effects of ajoene, which is an organosulfur compound derived from garlic, were investigated by the Ames test. Ajoene inhibited mutagenesis induced by both benzo[a]pyrene (B[a]P) and 4-nitro-1,2-phenylenediamine (NPD) in a dose-dependent manner. In particular, NPD-induced mutagenesis was more effectively suppressed by ajoene than the B[a]P-induced type. Furthermore, the inhibition of mutagenesis by ajoene was more effective for transition-type mutations than for the frame shift type. HPLC analysis of B[a]P metabolism in the presence of the rat liver microsomal fraction (S-9) showed that ajoene dose-dependently inhibited the metabolic activation of B[a]P. This suggests that ajoene affected the metabolic enzymes in the S-9 fraction.
Ishikawa
K
K
Bio Development Division, Nagoya Seiraku Co., Ltd., Japan.
Naganawa
R
R
Yoshida
H
H
Iwata
N
N
Fukuda
H
H
Fujino
T
T
Suzuki
A
A
D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D001711:Biotransformation / Q000187:drug effects; D004220:Disulfides / Q000302:isolation & purification / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity; D010655:Phenylenediamines / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013454:Sulfoxides
D016587:Antimutagenic Agents; D004220:Disulfides; D009153:Mutagens; D010655:Phenylenediamines; D010936:Plant Extracts; D013454:Sulfoxides; D001564:Benzo(a)pyrene; C012277:1,2-diamino-4-nitrobenzene; C048980:ajoene