title: The mammalian brain rsec6/8 complex.
abstract: rsec6 and rsec8 are two components of a 17S complex in mammalian brain that is homologous to the yeast 834 kDa Sec6/8/15 complex which is essential for exocytosis. Purification and partial amino acid sequencing of the mammalian rsec6/8 complex reveals that it is composed of eight novel proteins with a combined molecular weight of 743 kDa. The complex is broadly expressed in brain and displays a plasma membrane localization in nerve terminals. Membrane associated rsec6/8 complex coimmunoprecipitates with syntaxin, a plasma membrane protein critical for neurotransmission. These data suggest a role for the mammalian rsec6/8 complex in neurotransmitter release via interactions with the core vesicle docking and fusion apparatus.
authors.affiliation: Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical School, California 94305-5428, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Hsu
authors.forename: A E
authors.initials: AE
authors.lastname: Ting
authors.forename: C D
authors.initials: CD
authors.lastname: Hazuka
authors.forename: S
authors.initials: S
authors.lastname: Davanger
authors.forename: J W
authors.initials: JW
authors.lastname: Kenny
authors.forename: Y
authors.initials: Y
authors.lastname: Kee
authors.forename: R H
authors.initials: RH
authors.lastname: Scheller
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010449:Peptide Mapping; D011233:Precipitin Tests; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; C096102:Exoc3 protein, rat; C076326:Exoc4 protein, rat; D008565:Membrane Proteins; D011506:Proteins; D050765:Qa-SNARE Proteins