title: Immunohistochemical assessment of steroid hormone receptors in tissues of the anal canal. Implications for anal incontinence?
abstract: BACKGROUND
  A prerequisite for a hormonal influence on anal continence in women is the presence of hormone receptors in the tissues of the anal canal. Using immunohistochemical techniques, we demonstrated and localized estrogen and progesterone receptors (ER, PR) in tissue sections of the anal canal.
  
  
  METHODS
  Thirty-five specimens of the anorectal region from 21 patients (14 women, seven men) were examined histologically for smooth muscle (present in specimens from ten females and in seven males), striated muscle (present in three females and five males), and perimuscular connective tissue (present in 12 females and seven males). Immunostaining for ER and PR was then performed by incubating with primary anti-ER and anti-PR antibody and visualization of specific antibody binding by the ABC technique with DAB as chromogen.
  
  
  RESULTS
  Positive staining for ER and PR was seen exclusively over cell nuclei. Estrogen receptors were found in the smooth muscle cells of the internal sphincter of all females (10/10) and in four of the seven males. Staining for ER was detected in the perimuscular connective tissue of all females (12/12) and in four of the seven males. No specific staining for ER or PR was found in the nuclei of striated muscle cells of the external sphincter in males or females (n = 8). In females, about 50% of the ER-positive tissues were also found to be positive for PR. Amongst the male patients, only one exhibited staining for PR, and this was confined to the smooth muscle.
  
  
  CONCLUSIONS
  Expression of ER is a constitutional feature of the connective tissue and smooth muscle cells of the anal continence organ. Estrogen receptors and PR are not detectable in the striated muscle fibers of the external anal sphincter in either sex. The presence of ER in the stroma and smooth muscles of the anal canal suggests that these tissues are targets for estrogen. This constitutes a theoretical basis for the beneficial effects of estrogen and progestin replacement on anal continence in postmenopausal women.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tuebingen, Germany.
authors.forename: H B
authors.initials: HB
authors.lastname: Franz
authors.forename: D
authors.initials: D
authors.lastname: Wendler
authors.forename: G
authors.initials: G
authors.lastname: Oettling
mesh_terms: D001003:Anal Canal / Q000737:chemistry* / Q000378:metabolism; D003238:Connective Tissue / Q000737:chemistry / Q000378:metabolism; D005242:Fecal Incontinence / Q000209:etiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000032:analysis* / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone