A genetic study of a Staphylococus aureus plasmid involving cure and transference.
High frequency transfer and elimination of drug resistance may indicate an extrachromosomal inheritance of genetic determinants. This study shows the cure and transfer of a small plasmid and tetracycline resistance in Staphylococcus aureus 1030 (55)Tet strains. Several methods are available for plasmid elimination. We used ethidium bromide, an agent that binds to DNA, and thus inhibits DNA polymerase. This caused a high frequency of loss of the small plasmid and resistance to tetracycline. Transfer of tetracycline resistance was done in a mixed culture at a frequency of 10(-6). This type of study is very important to physicians and epidemiology investigators and provides better knowledge on antibiotic-resistance mechanisms that may occur in vivo in a hospital environment.
Darini
A L
AL
Department of Clinical, Toxicological and Bromatological Analysis, University of São Paulo, Brasil.
D003227:Conjugation, Genetic; D004996:Ethidium; D005111:Extrachromosomal Inheritance; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
D004996:Ethidium; D013752:Tetracycline; D013307:Streptomycin