title: Determination of fluvastatin enantiomers and the racemate in human blood plasma by liquid chromatography and fluorometric detection.
abstract: Liquid chromatographic methods for the determination of fluvastatin, as racemate and as separated enantiomers, are described. Fluvastatin was extracted at pH 6.0 from blood plasma into methyl tert.-butyl ether. The organic phase was evaporated and the extract redissolved into either a phosphate buffer solution of pH 6.0 containing tetrabutylammonium fluoride and methanol for the racemate determination, or in a mixture of acetonitrile and water for assaying the enantiomers. The absolute recoveries were 95 and 86% for the racemate and the enantiomers, respectively, and the limit of quantitation 0.5 nmol/1 for the racemate, and 5 nmol/l for the enantiomers, when using half a millilitre of plasma sample. The samples were chromatographed on a C8 column (racemate) and on a Chiralcel OD-R column (enantiomers), and monitored using fluorescence detection. In the achiral system, post-column exposure of the eluate to UV light enhanced the sensitivity by 4 to 5 times when compared with analysis based on the native fluorescence.
authors.affiliation: Astra Hässle AB, Mölndal, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Toreson
authors.forename: B M
authors.initials: BM
authors.lastname: Eriksson
mesh_terms: D000924:Anticholesteremic Agents / Q000097:blood*; D002853:Chromatography, Liquid; D005229:Fatty Acids, Monounsaturated / Q000097:blood*; D000077340:Fluvastatin; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D000924:Anticholesteremic Agents; D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D007211:Indoles; D000077340:Fluvastatin