Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme.
Yeast Rox3 protein, implicated by genetic evidence in both negative and positive transcriptional regulation, is identified as a mediator subunit by peptide sequence determination and is shown to copurify and co-immunoprecipitate with RNA polymerase II holoenzyme.
Gustafsson
C M
CM
Department of Structural Biology, Stanford University School of Medicine, California 94305-5400, USA.
Myers
L C
LC
Li
Y
Y
Redd
M J
MJ
Lui
M
M
Erdjument-Bromage
H
H
Tempst
P
P
Kornberg
R D
RD
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry*; D015966:Gene Expression Regulation, Fungal; D046911:Macromolecular Substances; D056892:Mediator Complex; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012319:RNA Polymerase II / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000737:chemistry*
D005656:Fungal Proteins; D046911:Macromolecular Substances; D056892:Mediator Complex; C103856:ROX3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II