Cyclopiazonic acid-induced changes in contractile activity of smooth muscle strips isolated from cat and guinea-pig stomach.
The effects of cyclopiazonic acid (CPA), a specific inhibitor of sarcoplasmic reticulum Ca(2+)-ATPase, on contractile activity of circular smooth muscle strips isolated from the antrum, corpus and fundus regions of the cat and guinea-pig stomach were studied. Contractile activity was recorded under isometric conditions, in organ baths. CPA, concentration dependently (3 x 10(-7)-3 x 10(-5) M) increased the tone of the cat and guinea-pig gastric fundus and corpus as well as the amplitude of the phasic contractions of the cat corpus and antrum, affecting their frequency. CPA had a dual action on the phasic contractions of the guinea-pig antrum: an increase at low concentrations (up to 10(-6) M) and inhibition at high concentrations (10(-6)-3 x 10(-5) M). Tetrodotoxin (10(-6) M), atropine (10(-6) M) and N omega-nitro-L-arginine (10(-4) M) did not change significantly the effects of CPA. Nifedipine completely inhibited the CPA-induced phasic contractions and partly inhibited the CPA-induced tonic contractions. The nitric oxide-releasing agents, sodium nitroprusside (10(-3) M) and 3-morpholino-sydnonimine (10(-3) M), completely inhibited the CPA-induced tonic and phasic contractions. CPA induced tonic contractions in the cat and guinea-pig gastric fundus precontracted by acetylcholine (10(-5) M) and inhibited the acetylcholine (10(-6) M)-induced phasic contractions in the guinea-pig gastric antrum and corpus. The results suggest multiple roles for sarcoplasmic reticulum Ca2+ stores and sarcoplasmic reticulum Ca(2+)-ATPase in the shaping of spontaneous and evoked tonic and phasic contractions of the stomach, and highlight important species and tissue differences.
Petkov
G V
GV
Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. gpetkov@iph.bio.acad.bg
Boev
K K
KK
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors* / Q000502:physiology; D002415:Cats; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology
D007211:Indoles; D014665:Vasodilator Agents; D009599:Nitroprusside; D019335:Nitroarginine; C002385:linsidomine; D008981:Molsidomine; D019001:Nitric Oxide Synthase; D000252:Calcium-Transporting ATPases; D000109:Acetylcholine; D002118:Calcium; C000543:cyclopiazonic acid