title: The structure of the exopolysaccharide produced by the halophilic Archaeon Haloferax mediterranei strain R4 (ATCC 33500).
abstract: The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as [formula: see text]
authors.affiliation: School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
authors.forename: H
authors.initials: H
authors.lastname: Parolis
authors.forename: L A
authors.initials: LA
authors.lastname: Parolis
authors.forename: I F
authors.initials: IF
authors.lastname: Boán
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez-Valera
authors.forename: G
authors.initials: G
authors.lastname: Widmalm
authors.forename: M C
authors.initials: MC
authors.lastname: Manca
authors.forename: P E
authors.initials: PE
authors.lastname: Jansson
authors.forename: I W
authors.initials: IW
authors.lastname: Sutherland
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000032:analysis; D001105:Archaea / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D010504:Periodic Acid / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D017421:Sequence Analysis; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013463:Sulfuric Acid Esters / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D013463:Sulfuric Acid Esters; D010504:Periodic Acid; C009288:metaperiodate; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine