Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression.
Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.
Cluster
P D
PD
John Innes Centre, Norwich Research Park, UK.
O'Dell
M
M
Metzlaff
M
M
Flavell
R B
RB
D015139:Blotting, Southern; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry*; D008040:Genetic Linkage; D005822:Genetic Vectors; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013489:Suppression, Genetic; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA; D004262:DNA Restriction Enzymes