Effects of in vivo culture of murine mammary adenocarcinoma cells on tumor and metastatic growth.
We studied the effect of tumoral microenvironments on metastatic phenotypes. Therefore, murine mammary adenocarcinoma cells cultured in vivo in diffusion chambers (DC) were implanted intraperitoneally in BALB/c mice. The behavior of DC-cultured cells was compared with that of cells obtained from tumors growing subcutaneously or intraperitoneally and from primary cultures in vitro of the former. DC-cultured and control cells were inoculated into normal mice to evaluate their tumorigenicity and metastasizing ability. We found that DC-cultured cells were less tumorigenic and metastatic both in spontaneous and in experimental metastasis assays. The host response to tumor progression resulted in an early leukocytosis, probably due to the overproduction of a hematopoietic factor by the tumor cells. Finally, it was found that DC-cultured cells produced lower levels of urokinase-type plasminogen activator activity, while no differences were found in the metalloproteinase production compared to cells obtained from a tumor growing subcutaneously.
Aguirre Ghiso
J
J
Research Area, Institute of Oncology, Angel H. Roffo, University of Buenos Aires, Argentina.
Diament
M
M
D'elia
I
I
Bal de Kier Joffé
E
E
Klein
S
S
D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology* / Q000556:secondary; D000818:Animals; D001772:Blood Cell Count; D015194:Diffusion Chambers, Culture / Q000379:methods; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology*; D008666:Metalloendopeptidases / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010641:Phenotype; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
D014568:Urokinase-Type Plasminogen Activator; D008666:Metalloendopeptidases