Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP.
Vimentin and glial fibrillary acidic protein (GFAP) are intermediate filament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphorylated by various protein kinases. Site- and phosphorylation-specific antibodies which recognize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.
Inagaki
N
N
Department of Biochemistry, Aichi Open Center Research Institute, Japan.
Tsujimura
K
K
Tanaka
J
J
Sekimata
M
M
Kamei
Y
Y
Inagaki
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D016203:CDC2 Protein Kinase / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D010750:Phosphoproteins; D014746:Vimentin; D011494:Protein Kinases; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase