Expression of 135-kDa insecticidal protein gene from Bacillus thuringiensis in the yeast Saccharomyces cerevisiae.
Bacillus thuringiensis subsp. aizawai produces 130-kDa and 135-kDa (CryIA(a)) insecticidal proteins. When Saccharomyces cerevisiae was transformed by the vector carrying a cryIA(a) gene, the gene expression could not be observed. When the 5'-upstream region from the initiation codon was removed using a synthetic oligonucleotide, the CryIA(a) protein was successfully synthesized in yeast. The yeast extract containing CryIA(a) protein had insecticidal activity against Plutella xylostella larvae.
Watanabe
K
K
Showa Woman's University, Tokyo, Japan.
Toyoda
M
M
Hashimoto
H
H
Nakagawa
K
K
Nakamura
K
K
Yabusaki
Y
Y
Tanimura
A
A
Saito
Y
Y
D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D006460:Hemolysin Proteins; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis