title: Antigenicity, pathogenicity, and immunogenicity of small and large plaque infectious bursal disease virus clones.
abstract: A serotype 1 variant strain of infectious bursal disease virus designated IN was passaged 40 times in BGM-70 cell line. A small plaque (SP) clone and a large plaque (LP) clone were then isolated and plaque purified four times. The SP and LP viruses formed circular plaques about 0.5 mm and 6.0 mm in diameter, respectively. Both clones lost their pathogenicity for specific-pathogen-free (SPF) chickens and did not elicit significant levels of virus-neutralizing antibody titers. However, the SP and LP clones maintained their immunogenicity when used as inactivated vaccines in SPF chickens. The restriction enzyme profiles of both clones were similar. Back passage of the SP and LP clones in SPF chickens resulted in loss of their phenotypic characteristics.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Hassan
authors.forename: C K
authors.initials: CK
authors.lastname: Nielsen
authors.forename: L A
authors.initials: LA
authors.lastname: Ward
authors.forename: D J
authors.initials: DJ
authors.lastname: Jackwood
authors.forename: Y M
authors.initials: YM
authors.lastname: Saif
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology*; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D002460:Cell Line; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D013154:Spleen / Q000737:chemistry; D015164:Vaccines, Inactivated / Q000627:therapeutic use; D010948:Viral Plaque Assay / Q000379:methods / Q000662:veterinary*; D014765:Viral Vaccines / Q000627:therapeutic use
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines