title: Somatic embryogenesis and plantlet from petal cultures of pomegranate, Punica granatum L.
abstract: Somatic embryos were induced through petal cultures on MS supplemented with various growth adjuvants individually or in different combinations. Numerous embryoids formed from friable callus on MS with 5 mg/L of IAA, IBA or NAA developed only roots, but those formed on MS with 5 mg/L of BAP or KN developed shoots. Explants reared on MS supplemented with IAA (5 mg/L) and BAP (5 mg/L) though differentiated numerous embryoids but developed multilobed or fused cotyledons. However, callus induced on MS with IAA (1 mg/L) or IBA (1 mg/L) on subculturing in the same medium with half-strength salts and sucrose (4%) produced both roots and shoots resulting in plantlets.
authors.affiliation: Department of Botany, Karnatak University, Dharwad, India.
authors.forename: K
authors.initials: K
authors.lastname: Nataraja
authors.forename: G K
authors.initials: GK
authors.lastname: Neelambika
mesh_terms: D046508:Culture Techniques; D005638:Fruit / Q000196:embryology*; D014197:Trees