title: Characterization of novel non-toxic Bacillus thuringiensis isolates from Korea.
abstract: Four Bacillus Thuringiensis isolates from soil samples produced parasporal inclusions which were non-toxic to insects. The isolates were named B. thuringiensis NTB-1, NTB-2, NTB-3 and NTB-4. The parasporal inclusions were shown to be ovoid by phase contrast and scanning electron microscopy. The serotypes of the four isolates were determined by agglutination using 33 antisera; NTB-1 and NTB-4 seemed to be subsp. israelensis, and NTB-2 seemed to be subsp. pondicheriensis. NTB-3 did not react with the 33 antisera. However, comparison of parasporal protein and plasmid DNA patterns of the four isolates with those of 15 known non-toxic B. thuringiensis strains demonstrated that the four isolates are novel.
authors.affiliation: Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Roh
authors.forename: H W
authors.initials: HW
authors.lastname: Park
authors.forename: B R
authors.initials: BR
authors.lastname: Jin
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Yu
authors.forename: S K
authors.initials: SK
authors.lastname: Kang
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007313:Insecta; D007723:Korea