Structural analysis of the glycosyl-phosphatidylinositol membrane anchor of the merozoite surface proteins-1 and -2 of Plasmodium falciparum.
Plasmodium falciparum accumulates the two merozoite surface proteins-1 and -2 during schizogony. Both proteins are proposed to be anchored in membranes by glycosyl-phosphatidylinositol membrane anchors. In this report the identity of these GPI-anchors is confirmed by labelling with tritiated precursors and additionally by specific enzymatic and chemical treatments. Detailed structural analysis of the core-glycans showed that the GPI-anchors of both proteins possess an extra alpha 1-2 linked mannose at the conserved trimannosyl-core-glycan. MSP-1 and MSP-2 labelled with tritiated myristic acid possess primarily radioactive myristic acid at inositol rings in both GPI-anchors. Additionally the hydrophobic fragments released from [3H]myristic acid labelled GPI-anchors were identified as diacyl-glycerols, carrying preferentially [3H]palmitic acid in an ester-linkage.
Gerold
P
P
Zentrum für Hygiene und Med. Mikrobiologie, Philipps-Universität Marburg, Germany.
Schofield
L
L
Blackman
M J
MJ
Holder
A A
AA
Schwarz
R T
RT
D000818:Animals; D000953:Antigens, Protozoan; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis; D011498:Protein Precursors / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*
D000953:Antigens, Protozoan; D002241:Carbohydrates; D005227:Fatty Acids; D017261:Glycosylphosphatidylinositols; D020066:Merozoite Surface Protein 1; D011134:Polysaccharides; D011498:Protein Precursors; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium