Polymer- versus silica-based separation media: elimination of nonspecific interactions in the chiral recognition process through functional polymer design.
A novel chiral stationary phase that contains a new chiral selector based on L-valine-3,5-dimethylanilide attached to monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads has been prepared. The polymeric separation medium provides greatly enhanced enantioselectivities and reduced retention times when compared to the analogous silica-based chiral stationary phase in the separation of the enantiomers of 3,5-dinitrobenzamido derivatives of alpha-amino acids under normal-phase HPLC conditions. Separation factors (alpha) of up to 7 were achieved with the polymeric separation medium, which is also useful for the separation of large samples under overload conditions.
Liu
Y
Y
Baker Laboratory, Department of Chemistry, Comell University, Ithaca, New York 14853-1301, USA.
Svec
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F
Fréchet
J M
JM
Juneau
K N
KN
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D011108:Polymers; D012822:Silicon Dioxide; D013237:Stereoisomerism
D011108:Polymers; D012822:Silicon Dioxide