Automation of selective assays for on-line bioprocess monitoring by flow-injection analysis.
On-line analysis of one component in a complex media used for bioprocesses requires the application of selective tests such as enzymes assays. Because these assays are susceptible to interference by other medium components and have a limited detection range, automatic sample pretreatment is a prerequisite. The progress made with automatic sample pretreatment in flow-injection analysis makes this technique particularly suitable for on-line monitoring of bioprocesses. Moreover, newly developed software control systems may improve the necessary robustness of flow-infection analysis systems.
van der Pol
J J
JJ
Institute of biotechnology, Forschungszentrum Jülich GmbH, Germany. van-der-Pol@chorbr.nestrd.ch
de Gooijer
C D
CD
Biselli
M
M
Wandrey
C
C
Tramper
J
J
D000818:Animals; D001709:Biotechnology; D002478:Cells, Cultured; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D009862:Online Systems
D004800:Enzymes, Immobilized