title: Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) phosphorylase activity.
abstract: Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) phosphorylase, previously only known in lower eukaryotes, has been detected in extracts of the cyanobacteria Anabaena flos-aquae, Anabaena variabilis and Synechococcus sp. The 32 kDa enzyme was partially purified from A. flos-aquae and separated from a 23 kDa hydrolytic activity. It had a pH optimum of 9.5 and required a bivalent cation for activity: Mg2+, Mn2+, Ca2+, Co2+ or Zn2+. Diadenosine tri-, tetra- and penta-phosphates were all effective substrates (relative rates 0.85, 1.00 and 0.27 respectively), while the hexaphosphate was a poor substrate and the diphosphate was inactive. ADP was always one of the products of phosphorolysis. Arsenate and vanadate could substitute for phosphate (relative rates 1.80, 2.25 and 1.00 respectively), but tungstate and sulphate could not. Chromate and molybdate were poor substrates. A search of the GenBank non-redundant database revealed a putative Ap4A phosphorylase gene in the cyanobacterium Synechocystis sp. The gene showed significant blocks of identity/similarity with yeast Ap4A phosphorylases I and II, particularly the latter.
authors.affiliation: School of Biological Sciences, University of Liverpool, U.K.
authors.forename: A G
authors.initials: AG
authors.lastname: McLennan
authors.forename: E
authors.initials: E
authors.lastname: Mayers
authors.forename: D G
authors.initials: DG
authors.lastname: Adams
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D017033:Anabaena / Q000201:enzymology*; D000838:Anions / Q000378:metabolism; D002413:Cations, Divalent / Q000494:pharmacology; D002850:Chromatography, Gel; D000458:Cyanobacteria / Q000201:enzymology*; D016208:Databases, Factual; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D000838:Anions; D002413:Cations, Divalent; D009711:Nucleotides; D000244:Adenosine Diphosphate; D009713:Nucleotidyltransferases; C044740:diadenosine 5,5'''-P(1),P(4)-tetraphosphate alpha,beta-phosphorylase