Elastase-induced hydrolysis of synthetic solid substrates: poly(ester-urea-urethane) and poly(ether-urea-urethane).
Human neutrophil elastase (HNE) and porcine pancreatic elastase (PPE) were incubated with two radiolabelled model poly(urethane), a poly(ester-urea-urethane) containing [14C]toluene diisocyanate ([14C]TDI), poly(caprolactone)(PCL) and ethylenediamine (ED), and a poly(ether-urea-urethane) containing [14C]TDI, poly(tetramethylene oxide) (PTMO) and ED. Ten-fold more radioactive carbon was released when PPE was incubated with [14C]TDI/PCL/ED than when HNE was used. The PPE-induced radioactive carbon release was significantly reduced by a specific elastase inhibitor. Ten-fold less radioactive carbon was released when [14C]TDI/PTMO/ED was incubated with PPE as compared to [14C]TDI/PCL/ED. Since neutrophils, which contain elastolytic activity, are present during the inflammatory response, the stability of biomaterials used in implanted devices may be affected.
Labow
R S
RS
Cardiovascular Devices Division, University of Ottawa Heart Institute, ON, Canada.
Erfle
D J
DJ
Santerre
J P
JP
D000818:Animals; D002250:Carbon Radioisotopes; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology
D002250:Carbon Radioisotopes; D004791:Enzyme Inhibitors; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; C045786:polyurea; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D014357:Trypsin