Expression of a 70-kDa heat-shock-related protein during transformation from free-living infective larvae to the parasitic stage in Strongyloides venezuelensis.
The in vitro transformation system of Strongyloides venezuelensis has been established which induces free-living infective larvae to transform into the parasitic stage by a temperature shift from 25 to 37 degrees C. Comparison of the profiles of proteins labeled with [35S]-methionine in infective larvae at 25 and 37 degrees C with marked morphological transformation revealed an increase in the 70-kDa protein, thus showing these proteins to be related to the family of heat-shock proteins. In addition, the new appearance of two complexes between 16-kDa and 22-kDa proteins was observed. The 70-kDa protein cross-reacted with monoclonal antibody against human heat-shock protein 70, which was constitutively expressed. These proteins, synthesized during the transformation of S. venezuelensis from the infective larval stage to the parasitic stage, might be crucial for biochemical events that regulate the infectivity of the parasite for the host.
Tsuji
N
N
National Institute of Animal Health, Ibaraki, Japan. tsjin@niah.affrc.go.jp
Ohta
M
M
Fujisaki
K
K
D000818:Animals; D000909:Antibodies, Helminth; D015153:Blotting, Western; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000276:immunology; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D009024:Morphogenesis; D013321:Strongyloides / Q000254:growth & development* / Q000378:metabolism*
D000909:Antibodies, Helminth; D018840:HSP70 Heat-Shock Proteins; D015801:Helminth Proteins