A single vector cloning, mutagenesis and expression system.
We describe a multipurpose Escherichia coli vector, pOTSf1blue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authentic protein overexpression and efficient mutagenesis.
Towler
E M
EM
Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
Stebbins
J W
JW
Debouck
C
C
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018741:Replication Origin / Q000235:genetics
D004277:DNA, Single-Stranded