title: Antibody reactivities to glutamate-rich peptides of Plasmodium falciparum parasites in humans from areas of different malaria endemicity.
abstract: Synthetic P. falciparum peptides were evaluated as tools in epidemiological investigations of malaria. Plasma IgM and IgG antibody reactivities against synthetic peptides covering sequences of glutamate-rich protein (GLURP) and acidic-basic repeat antigen (ABRA) were measured by ELISA in individuals from malaria-endemic areas of Sudan, Indonesia and The Gambia to study antibody responses to these peptides in donors living in areas of different malaria endemicity. IgG and IgM reactivities to the peptides increased with malaria endemicity, although there were no differences in reactivities to the GLURP peptide between non-exposed donors and donors living in areas of low malaria endemicity. IgG reactivities to the GLURP peptide in Sudanese adults were high one month after treatment in all adults tested, while IgG reactivities to the ABRA peptide were infrequent. IgM responses to the peptides tested were shortlived in most patients. In Gambian children with malaria, IgM reactivities but not IgG antibody reactivities against the ABRA peptide were higher in those with mild malaria than in those with severe malaria. The peptides may be useful in future epidemiological studies, especially in areas of low malaria endemicity.
authors.affiliation: Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), Denmark.
authors.forename: P H
authors.initials: PH
authors.lastname: Jakobsen
authors.forename: T G
authors.initials: TG
authors.lastname: Theander
authors.forename: L
authors.initials: L
authors.lastname: Hviid
authors.forename: S
authors.initials: S
authors.lastname: Morris-Jones
authors.forename: J B
authors.initials: JB
authors.lastname: Jensen
authors.forename: R A
authors.initials: RA
authors.lastname: Bayoumi
authors.forename: B M
authors.initials: BM
authors.lastname: Greenwood
authors.forename: I C
authors.initials: IC
authors.lastname: Bygbjerg
authors.forename: P M
authors.initials: PM
authors.lastname: Heegaard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan; D003718:Denmark / Q000453:epidemiology; D019353:Endemic Diseases; D005260:Female; D005714:Gambia / Q000453:epidemiology; D005971:Glutamates / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008137:Longitudinal Studies; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000138:chemical synthesis / Q000276:immunology*; D013397:Sudan / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D005971:Glutamates; D015800:Protozoan Proteins; C079596:p101-ABRA protein, Plasmodium falciparum; C072492:glutamate-rich protein, Plasmodium