Refining a proximal breakpoint cluster at chromosome 3p11.2 in non-papillary renal cell carcinomas.
Our previous cytogenetic and microsatellite analyses showed a terminal deletion of chromosome 3p segments with a breakpoint region between 3p11.2 and 3p14.1 bands in 98% of non-papillary renal cell carcinomas (RCC). This breakpoint region covers approx. 20-25 cM genetic distance. Earlier, we found a higher frequency of rearrangements at chromosome 3p11.2 and have therefore now analyzed 74 sporadic and 26 VHL-associated RCCs with 6 polymorphic microsatellite markers mapped to this region. Thirty-three per cent of the breakpoints were mapped to a genetic distance of less than one-twentieth of the large breakpoint region 3p11.2-14.1. We suggest that unstable DNA sequences at chromosome 3p11.2 serve as a genetic basis for deletions and homologous and non-homologous recombinations to remove distal 3p sequences with one copy of RCC gene(s) in different types of tumors. We have identified a YAC clone 909d5 that spans the most frequently occurring breaks between loci D3S1251, D3S1101, and D3S1552 and allows this region to be cloned.
Bugert
P
P
Clinical Research Group in Molecular Oncology, Department of Urology, Ruprecht-Karls-University, Heidelberg, Germany.
Kenck
C
C
Wilhelm
M
M
Kovacs
G
G
D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping / Q000379:methods; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000737:chemistry; D004276:DNA, Satellite / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D017353:Gene Deletion; D005819:Genetic Markers / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D005819:Genetic Markers