Effect of cyclic GMP produced by natriuretic peptides on osteoblast-like MC3T3-E1 cells.
The C-type natriuretic peptide (10(-7) M) and atrial natriuretic peptide (10(-7) M) enhanced cGMP accumulation by 418 and 83 times the control value, respectively, in osteoblast-like MC3T3-E1 cells. The natriuretic peptide B receptor was assumed to be the major natriuretic peptide receptor. 8-Bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) activated alkaline phosphatase doubled the activity versus the control value on day 15. Phosphodiesterase activity was not stimulated by the addition of cGMP (1 MicroM). cGMP-dependent protein kinase (G kinase) activity of the supernatant fraction was 25.5 pmol/min/mg protein. The 42 kDa protein band was detected to be phosphorylated by G kinase on SDS-PAGE. These results supported the hypothesis that natriuretic peptides regulate the differentiation of MC3T3-E1 cells through a cGMP-dependent pathway.
Nashida
T
T
Department of Oral Biochemistry, Nippon Dental University School of Dentistry at Niigata, Japan.
Matsumoto
H
H
Imai
A
A
Kameda
A
A
Shimomura
H
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002460:Cell Line; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D051379:Mice; D020098:Natriuretic Peptide, C-Type; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology*
D011506:Proteins; D020098:Natriuretic Peptide, C-Type; C016276:8-bromocyclic GMP; D009320:Atrial Natriuretic Factor; D017869:Cyclic GMP-Dependent Protein Kinases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP