Steroidal glycosides from the underground parts of Hosta plantaginea var. japonica and their cytostatic activity on leukaemia HL-60 cells.
A new C22-steroid glycoside was isolated from the underground parts of Hosta plantaginea var. japonica, together with a known furostanol saponin and three known spirostanol saponins. The structure of the new steroid glycoside was characterized by spectroscopic analysis and acid-catalysed hydrolysis as 2 alpha, 3 beta, 16 beta-trihydroxy-5 alpha-pregn-20(21)-ene-carboxylic acid gamma-lactone 3-O-¿O-beta-D-glucopyranosyl-(1-->2)-O-beta-D-glucopyranosyl-(1--> 4)-beta-D-galactopyranoside¿. The isolated compounds were assayed for their cytostatic activity on leukaemia HL-60 cells. The spirostanol saponins showed cytostatic activity in a dose-dependent manner with the IC50 values ranging between 1 and 3 micrograms ml-1.
Mimaki
Y
Y
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Kameyama
A
A
Kuroda
M
M
Sashida
Y
Y
Hirano
T
T
Oka
K
K
Koike
K
K
Nikaido
T
T
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D018922:HL-60 Cells / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010936:Plant Extracts; D018517:Plant Roots; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; D013256:Steroids