[Thin-layer chromatography analysis of methamphetamine in urine samples].
The paper investigated the chromatographic conditions for TLC analysis of metamphetamine on silica gel and the lipophilic stationary phase RP V 18 in order to qualitatively analyze the drug in urine samples. Attention was also paid to chemical detection. Five detection reagents were tested, out of which Fast Black K salt, yielding orange-red spots with a detection limit of 1 microgram, proved to be the best. In the analysis of metamphetamine in the samples of model urine, the best results were achieved on Kieselgel. Using the developing system ethyl acetate-ethanol-concentrated solution of ammonia (36:2:2), a complete separation of the metamphetamine spot from the spots of ballast from the biological matrix was achieved.
Klimes
J
J
Katedra farmaceutické chemie a kontroly léciv Farmaceutické fakulty Univerzity Karlovy, Hradec Králové.
Pilarová
P
P
D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D008694:Methamphetamine / Q000652:urine*; D015813:Substance Abuse Detection
D008694:Methamphetamine