title: Distribution and hormonal regulation of androgen receptor immunoreactivity in the forebrain of the male European ferret.
abstract: The distribution and hormonal regulation of androgen-receptor-immunoreactive (AR-ir) cells in the male European ferret forebrain were examined. AR-ir cells were found in many limbic and hypothalamic structures, and their distribution was similar to that reported for cells that either bind androgen or contain AR protein or mRNA in other species. Regulation of brain AR immunoreactivity by gonadal steroids was brain-region dependent. In most regions examined, including the preoptic area, amygdala, and several hypothalamic nuclei, castration reduced the density of AR-ir profiles and the intensity of immunocytochemical staining, and long-term (days) androgen, but not estrogen, replacement restored these parameters of AR immunoreactivity. Other areas, such as the bed nucleus of the stria terminalis, appeared to be relatively resistant to modulation of AR immunoreactivity by castration and long-term androgen treatment. The ability of testosterone to increase AR-ir profile density is not a simple consequence of translocation of AR from the cytoplasm to the nucleus, because short-term (hours) treatment with testosterone did not result in an increase in AR-ir profile density equivalent to that seen after 10 days of testosterone treatment. Thus, androgens appear to be able to increase AR levels within certain brain cell groups, thereby altering target tissue responsiveness to their own action.
authors.affiliation: Neuroscience Program, Michigan State University, East Lansing 48824, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Kashon
authors.forename: J A
authors.initials: JA
authors.lastname: Arbogast
authors.forename: C L
authors.initials: CL
authors.lastname: Sisk
mesh_terms: D000818:Animals; D001931:Brain Mapping; D005289:Ferrets; D007150:Immunohistochemistry; D008297:Male; D016548:Prosencephalon / Q000378:metabolism* / Q000502:physiology*; D011944:Receptors, Androgen / Q000378:metabolism*
chemical_list: D011944:Receptors, Androgen