Acid lipase inhibitor in chicken plasma identified as apolipoprotein A-I.
We have reported a inhibitor of acid lipases in liver lysosomes and erythrocytes from chickens [M. Fujii et al., Int. J. Biochem., 22, 895-898 (1990)]. In this paper, the properties of the inhibitor were described in comparison with those of apo A-I of chicken. The purified inhibitor migrated with the same mobility on SDS-PAGE as apo A-I, and had a molecular weight of 27,000. The peptide map from the lipase inhibitor was similar to that of apo A-I. Antibodies to the acid lipase inhibitor also reacted with apo A-I. Apo A-I inhibited the acid lipase activities of liver lysosomes and erythrocytes from chickens as strongly as the lipase inhibitor. The N-terminal amino acid sequence of lipase inhibitor was identical to that of apo A-I as far as residue 20. The amino acid sequence of peptides obtained from the inhibitor by cleavage with CNBr corresponded to internal sequence of apo A-I, and so the CNBr-peptides were derived by cleavage after the methionine residues in apo A-I. The findings showed that the inhibitor of the acid lipases in liver lysosomes and erythrocytes from chickens was identical to apo A-I.
Fujii
M
M
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Japan.
Higuchi
T
T
Mukai
S
S
Yonekura
M
M
Yano
T
T
Kawaguchi
H
H
Nonaka
K
K
Fukunaga
T
T
Sugimoto
Y
Y
Yamada
S
S
D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002645:Chickens; D003600:Cytosol / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000737:chemistry; D008049:Lipase / Q000037:antagonists & inhibitors*; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D016632:Apolipoprotein A-I; D001798:Blood Proteins; D004791:Enzyme Inhibitors; D008049:Lipase