title: Borna disease virus RNA in peripheral blood mononuclear cells obtained from healthy dairy cattle.
abstract: We examined natural infection of Borna disease virus (BDV) in 74 healthy dairy cattle in Japan. The seroprevalence determined using recombinant BDV p24 protein was 20.3%. The prevalence of BDV RNA in peripheral blood mononuclear cells (PBMCs) determined by polymerase chain reaction for p24 was 10.8%. Cell fraction of the PBMCs revealed the presence of BDV RNA in the T cell-enriched fraction. Thus, BDV infection is widespread even among healthy cattle.
authors.affiliation: Section of Serology, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Nakaya
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Asahi
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: C
authors.initials: C
authors.lastname: Ishihara
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D001483:Base Sequence; D001891:Borna disease virus / Q000235:genetics*; D002417:Cattle / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood*
chemical_list: D010750:Phosphoproteins; D012367:RNA, Viral