Comparison of the properties of native and pentaammineruthenium(III)-modified xylanase.
Two xylanases, xynA of Bacillus pumilus and xyn II of Trichoderma reesei, were purified and then modified by the attachment of pentaammineruthenium, thereby resulting in the generation of a xylanase with veratryl alcohol oxidase activity. Hydrolytic activity of T. reesei xyn II on soluble xylans was unchanged by modification with pentaammineruthenium; however, modification of B. pumilus xynA greatly reduced xylan hydrolysis unless the active site of the xylanase was protected with xylose during the modification. The presence of histidine, cysteine, or reduced glutathione during xylan hydrolysis greatly increased the xylanase activity of the pentaammineruthenium-modified B. pumilus xylanase. Glycine, glutamic acid, methionine, or oxidized glutathione had no effect on xylanase activity.
Evans
B R
BR
Oak Ridge National Laboratory, TN 37831, USA.
Lane
L M
LM
Margalit
R
R
Hathaway
G M
GM
Ragauskas
A
A
Woodward
J
J
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000588:Amines / Q000378:metabolism; D000596:Amino Acids / Q000494:pharmacology; D001419:Bacteria / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D017975:Ruthenium Compounds / Q000378:metabolism*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014994:Xylose / Q000494:pharmacology; D014995:Xylosidases / Q000302:isolation & purification / Q000378:metabolism*
D000588:Amines; D000596:Amino Acids; D010455:Peptides; D017975:Ruthenium Compounds; D014990:Xylans; D006639:Histidine; D014994:Xylose; D000429:Alcohol Oxidoreductases; C057990:veratryl alcohol oxidase; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D005978:Glutathione; D003545:Cysteine