Subregion- and cell type-restricted gene knockout in mouse brain.
Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.
Tsien
J Z
JZ
Howard Hughes Medical Institute, Center for Learning and Memory, Massachusetts Institute of Technology, Cambridge 02139, USA.
Chen
D F
DF
Gerber
D
D
Tom
C
C
Mercer
E H
EH
Anderson
D J
DJ
Mayford
M
M
Kandel
E R
ER
Tonegawa
S
S
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005865:Gestational Age; D006624:Hippocampus / Q000502:physiology*; D019426:Integrases / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014764:Viral Proteins
D012333:RNA, Messenger; D014764:Viral Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C045073:Cre recombinase; D019426:Integrases