title: Urokinase-type plasminogen activator-deficient mice are predisposed to staphylococcal botryomycosis, pleuritis, and effacement of lymphoid follicles.
abstract: Urokinase-type plasminogen activator (uPA) is thought to be an important mediator in the proteolytic degradation of extracellular matrix components observed in a wide variety of normal physiological and pathological conditions. However, the phenotype of a recently developed strain of urokinase-deficient (uPA-/-) mice appears to be normal when maintained under ideal nonstressful conditions. We report an outbreak of botryomycosis, an unusual staphylococcal infection, in a colony of uPA-deficient mice. A detailed histological examination of these uPA-deficient animals also revealed a variety of previously unreported phenotypic abnormalities such as pleuritis and the effacement of lymphoid follicles in the regional lymph nodes and spleen. Additional phenotypic abnormalities such as dystrophic calcifications and rectal prolapse were also observed in the uPA-deficient population. These abnormalities were also noted in ostensibly healthy uPA-deficient animals. Botryomycosis did not affect a colony of wild-type (uPA+/+) animals maintained concurrently under identical conditions in the same room. The peculiar predisposition of the uPA-deficient animals to this rare bacterial infection and the development of phenotypic abnormalities associated with the targeted disruption the uPA gene suggests that uPA contributes significantly to the cutaneous microenvironment and is additional evidence of the extensive involvement of the plasminogen activators in mammalian physiology.
authors.affiliation: Department of Surgery, New York University Medical Center 10016, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Shapiro
authors.forename: J G
authors.initials: JG
authors.lastname: Duquette
authors.forename: I
authors.initials: I
authors.lastname: Nunes
authors.forename: D F
authors.initials: DF
authors.lastname: Roses
authors.forename: M N
authors.initials: MN
authors.lastname: Harris
authors.forename: E L
authors.initials: EL
authors.lastname: Wilson
authors.forename: D B
authors.initials: DB
authors.lastname: Rifkin
mesh_terms: D000038:Abscess / Q000473:pathology; D000818:Animals; D002465:Cell Movement; D004198:Disease Susceptibility; D008168:Lung / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D008221:Lymphoid Tissue / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D010998:Pleurisy / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D012005:Rectal Prolapse / Q000473:pathology; D013154:Spleen / Q000473:pathology; D013207:Staphylococcal Skin Infections / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D013211:Staphylococcus aureus / Q000302:isolation & purification; D014568:Urokinase-Type Plasminogen Activator / Q000172:deficiency* / Q000235:genetics*
chemical_list: D014568:Urokinase-Type Plasminogen Activator