[Purification of acetylcholinesterase from human erythrocytes using metal-chelate affinity chromatography].
Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. The best results (a 14-fold purification and more than 30% activity yield) were obtained with Zn2+ used as a complex-forming metal. The preparation had a specific activity of approximately 7 U. Its purity was tested by polyacrylamide gel electrophoresis under denaturing conditions.
Il'ina
A V
AV
Bannikova
G E
GE
Varlamov
V P
VP
Agabekian
R S
RS
D000110:Acetylcholinesterase / Q000097:blood / Q000302:isolation & purification*; D002614:Chelating Agents / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008670:Metals / Q000737:chemistry
D002614:Chelating Agents; D008670:Metals; D000110:Acetylcholinesterase