title: Expression of two cDNAs encoding class I chitinases of rice in Escherichia coli.
abstract: Two cDNAs encoding class I chitinases of rice were expressed in Escherichia coli. The cDNAs were fused to the MS2-polymerase gene in an expression vector, pEx31. The fusion proteins, expressed under the control of the lambda PL-promoter, showed the chitinase activity independent of the existence of the hevein domain. The enzymatic hydrolysis of colloidal chitin by the fusion proteins showed that the proteins were endo-type enzymes.
authors.affiliation: Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Pan
authors.forename: S L
authors.initials: SL
authors.lastname: Rhim
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
mesh_terms: D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005815:Genetic Code; D005822:Genetic Vectors; D012275:Oryza / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D002688:Chitinases