Characterization of a tRNA(Lys)(CUU) gene located in the opposite orientation upstream of a ZmFer2 ferritin gene in the maize nuclear genome.
The first evidence for a plant tRNA(Lys)(CUU) gene is reported. This gene is found closely linked 400 bp upstream, and on the complementary strand, of a ZmFer2 ferritin gene in the maize nuclear genome. Southern blot analysis indicates that this tRNA(Lys) is a member of a multigene family. This gene does not contain any intron, and exhibits classical intragenic regulatory elements found in eukaryotic tRNA genes (A and B boxes). Moreover, 5' and 3'-flanking sequences display typical features found in nuclear encoded tRNAs. The deduced mature tRNA sequence is almost identical to the sequence of a cytoplasmic tRNA(Lys)(CUU) from wheat germ. The maize tRNA(Lys) gene is expressed in vivo in maize and in transgenic tobacco, as shown by RT-PCR analysis.
Fobis-Loisy
I
I
Centre National de la Recherche Scientifique (Unité de Recherche 2133), Institut National de la Recherche Agronomique, Montpellier, France.
Briat
J F
JF
D001483:Base Sequence; D015139:Blotting, Southern; D002467:Cell Nucleus; D005293:Ferritins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012357:RNA, Transfer, Lys / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
D012357:RNA, Transfer, Lys; D005293:Ferritins