Measurements by fluorescence microscopy of the time-dependent distribution of meso-tetra-hydroxyphenylchlorin in healthy tissues and chemically induced "early" squamous cell carcinoma of the Syrian hamster cheek pouch.
The influence of the time interval between dye administration and detection by fluorescence microscopy was assessed in "early" squamous cell carcinomas of the cheek pouch mucosa and different healthy tissues of the Syrian hamster. Following intracardiac injection of 0.15 mg (kg bodyweight)-1 of meso-tetra-hydroxyphenylchlorin (m-THPC), groups of three animals were sacrificed at different time intervals up to 30 days. A group of three non-injected animals was used to detect the endogene fluorescence of the corresponding normal tissues for autofluorescence subtraction. The following excised organs (oesophagus, trachea, liver, spleen, kidney, skin, striated muscle, healthy and tumoral cheek pouch mucosae) were fast frozen in liquid nitrogen and stored at -70 degrees C for fluorescence microscopy. The results show significant differences in the detectable m-THPC levels in different tissue layers (for instance, the epithelia and muscle of the oesophagus, trachea and cheek pouch) at different time intervals. These data indicate that pharmacokinetic studies may be useful for selecting the optimal time for the photodetection and phototherapy of cancer.
Andrejevic
S
S
Department of Otolaryngology, Head and Neck Surgery, CHUV Hospital, Lausanne, Switzerland.
Savary
J F
JF
Monnier
P
P
Fontolliet
C
C
Braichotte
D
D
Wagnières
G
G
van den Bergh
H
H
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism*; D002610:Cheek; D006224:Cricetinae; D004947:Esophagus / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008652:Mesoporphyrins / Q000493:pharmacokinetics*; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000378:metabolism; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D008652:Mesoporphyrins; D011838:Radiation-Sensitizing Agents; C072269:temoporfin