Metabolic fate of oleic acid derived from lysosomal degradation of cholesteryl oleate in human fibroblasts.
Low density lipoprotein cholesteryl [14C]oleate (LDL-[14C]CO) was used as a tool to label lysosomes with cholesteryl [14C]oleate (CO) and to follow subsequently the metabolic processing of oleic acid released by acid lipase. Liberated [14C]oleate was incorporated into glycerolipids, mainly into phosphatidylcholine. Incubations in the presence of various concentrations of exogenously added oleic acid and double label experiments showed that oleic acid derived from lysosomal degradation of CO and exogenously added oleic acid distributed in a similar fashion among triacylglycerol and various phospholipids. To further study the metabolism of LDL-derived oleic acid, experiments were performed in which fibroblasts were prelabeled with LDL-[14C]CO. The subsequent processing of lysosome-derived oleic acid was followed with time without LDL-[14C]CO in the medium. From these experiments it became clear that apart from the esterification into glycerolipids a substantial part of lysosome-derived oleic acid was released into the medium. The efflux of oleic acid into the medium preceded the incorporation into glycerolipids, was dependent on the composition of the extracellular medium, and was energy-independent. Our data are compatible with a mechanism in which lysosome-derived fatty acids are transported to the plasma membrane prior to transport to endoplasmic reticulum for esterification. Intra- and extra-cellular factors influence the distribution of lysosome-derived oleic acid among cells and medium.
Groener
J E
JE
Department of Pediatrics, Leiden University Hospital, The Netherlands.
Bax
W
W
Poorthuis
B J
BJ
D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000494:pharmacology; D008247:Lysosomes / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D002788:Cholesterol Esters; D008078:Cholesterol, LDL; D004791:Enzyme Inhibitors; D010743:Phospholipids; D014280:Triglycerides; D019301:Oleic Acid; C014409:cholesteryl oleate; D011374:Progesterone; D013110:Sphingosine; D007099:Imipramine; C005682:safingol