Adhesion of bovine enterotoxigenic Escherichia coli (ETEC) by type 1-like fimbriae.
Enterotoxigenic Escherichia coli (STa+) strains were isolated from adult bovine with diarrhea. These strains did not express any known ETEC-specific adhesins. Although hemagglutination with rat and sheep erythrocytes was observed in the presence of D-mannose (MRHA), these strains also showed mannose-sensitive hemagglutination (MSHA) with guinea-pig erythrocytes. Electron microscopic studies revealed the presence of fimbria-like structures (provisionally called "F43ms") on bacterial cells grown at 37 degrees C but not on cells grown at 18 degrees C. However, it was observed by SDS-PAGE that the J-1 strain (F43ms+) produces a protein similar to F1 fimbriae, and this strain hybridized with a DNA probe for F1 fimbriae. Immunogold-labelling techniques indicated that a rabbit anti-serum is specific for F43ms fimbrial structures, but not for Type 1 fimbriae. The immunofluorescence test carried out with semipurified F43ms on bovine brush borders suggests that the fimbria-like structures are responsible for the adhesion to bovine epithelial cells.
Catani
C F
CF
Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, São Paulo, Brazil.
Yamada
A T
AT
Vidotto
M C
MC
Yano
T
T
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004926:Escherichia coli / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D010861:Fimbriae, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008358:Mannose / Q000494:pharmacology; D008854:Microscopy, Electron; D011817:Rabbits; D051381:Rats; D012756:Sheep
D008358:Mannose