title: Determination of timiperone in rat plasma by high-performance liquid chromatography with electrochemical detection.
abstract: We report a sensitive new method for the determination of timiperone in rat plasma by using high-performance liquid chromatography with electrochemical detection. The method involves extraction of plasma samples with heptane-isoamyl alcohol at pH > 8, followed by back-extraction into dilute acetic acid. Separation was accomplished by reversed-phase high-performance liquid chromatography on an ODS column with the mobile phase consisting of 0.1 M phosphate buffer (pH 3.5)-acetonitrile-methanol (65:20:15, v/v). Recovery was greater than 80%. Calibration curve was linear over the concentration range 0.5-50.0 ng/ml. The limit of quantitation of timiperone was 0.5 ng/ml plasma.
authors.affiliation: Research Department, Saitama Daiichi Pharmaceutical Co., Ltd., Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takayasu
authors.forename: I
authors.initials: I
authors.lastname: Kakubari
authors.forename: A
authors.initials: A
authors.lastname: Fukamachi
authors.forename: E
authors.initials: E
authors.lastname: Mafune
authors.forename: N
authors.initials: N
authors.lastname: Takasugi
authors.forename: K
authors.initials: K
authors.lastname: Takayama
authors.forename: T
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002021:Buffers; D002090:Butyrophenones / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013134:Spiperone / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D002021:Buffers; D002090:Butyrophenones; D013134:Spiperone; C024159:timiperone