title: Phospholipid hydroperoxide glutathione peroxidase (PHGPx) in rat testis nuclei is bound to chromatin.
abstract: In rat testis nuclei the activity of the selenoenzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx, EC 1.11.1.12) is much higher than in other tissues and subcellular compartments, with the sole exception of mitochondria. In nuclei, the bound enzyme is solubilized by DNase I treatment, thus suggesting a binding to chromatin. Treatment with ionic strength releases about 70% of bound PHGPx, suggesting that electrostatic bonds are involved. Immunogold electron microscopy indicates the association of PHGPx with chromatin structures in isolated nuclei. A possible interpretation of these data is a PHGPx protective role against DNA peroxidative damage. Furthermore, in agreement with kinetic and structural information, PHGPx-chromatin binding could suggest an hypothetical thiol oxidase activity toward specific thiol bearing proteins which could substitute for GSH as alternative donor substrates. Such activity could give to the enzyme a new important function which is not only protective but also has a specific regulatory function in chromatin condensation.
authors.affiliation: Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, 1-34127, Italy. panfili@univ.trieste.it
authors.forename: C
authors.initials: C
authors.lastname: Godeas
authors.forename: F
authors.initials: F
authors.lastname: Tramer
authors.forename: F
authors.initials: F
authors.lastname: Micali
authors.forename: A
authors.initials: A
authors.lastname: Roveri
authors.forename: M
authors.initials: M
authors.lastname: Maiorino
authors.forename: C
authors.initials: C
authors.lastname: Nisii
authors.forename: G
authors.initials: G
authors.lastname: Sandri
authors.forename: E
authors.initials: E
authors.lastname: Panfili
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D055672:Static Electricity; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism; D013737:Testis / Q000201:enzymology*
chemical_list: D002843:Chromatin; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase