title: Sequence analysis of an operon of a NAD(P)-reducing nickel hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803 gives additional evidence for direct coupling of the enzyme to NAD(P)H-dehydrogenase (complex I).
abstract: The sequence of a NAD(P)-reducing hydrogenase operon of Synechocystis sp. PCC 6803 containing genes for a small and a large hydrogenase subunit and six additional ORFs was determined. Until now only 11 of the 14 polypeptides of the NADH-dehydrogenase of E. coli were found in Synechocystis. By sequence homologies we suggest that the missing subunits of the peripheral part of the dehydrogenase, containing most of the FeS-clusters, are encoded by three ORFs of this operon. This hypothesis is discussed in relation to the NAD(P)-reducing hydrogenase of Synechocystis.
authors.affiliation: Philipps-Universität, Marburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Appel
authors.forename: R
authors.initials: R
authors.lastname: Schulz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry*; D016366:Open Reading Frames; D009876:Operon; D010088:Oxidoreductases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D010088:Oxidoreductases; C102841:NAD(P)-reducing nickel hydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone)