Catalase against met-Hb excess during oximetries of dilute Hb-A samples.
Functional parameters of diluted Hb-A have been determined before and after addition of catalase and disodium-EDTA to the samples. There are no important differences between the results drawn from catalase added samples and catalase free ones, except for the fact the met-Hb level at pH 7.8 is significantly lower in the samples containing catalase. On the contrary, catalase is almost ineffective against met-Hb at pH 6.8, whereas its activity at pH 7.3 is rather modest. Another limitation is that catalase remains active against met-Hb for not more than 15-20 minutes after addition to the sample, which is just the time necessary for one complete (manual) oximetry.
Ricco
G
G
Dipartimento di Scienze Biomediche ed Oncologia Umana, Università di Torino.
David
O
O
Lanza
C
C
Perfetto
F
F
Rabino-Massa
E
E
D002374:Catalase; D006441:Hemoglobin A / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010092:Oximetry / Q000379:methods*; D013053:Spectrophotometry
D006441:Hemoglobin A; D002374:Catalase