Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages.
A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of Fc receptor, phagocytic activities in U937. Furthermore, U937 cells, which were activated with STL-1 exhibited cytotoxic activity against human lung carcinoma cell line A549. However, STL-1 did not affect growth of a normal human fetal lung cell line TIG-1. The individual components of STL-1, neither sugar moiety nor fatty acids in the free form, were effective at inducing the differentiation of U937 cell. From these results, we concluded that STL-1 has low cytotoxicity against normal human cells and the ester molecule itself is responsible for the activity of inducing differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophage which results in the stimulation of the production of some cytotoxic substances.
Isoda
H
H
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Shinmoto
H
H
Matsumura
M
M
Nakahara
T
T
D015415:Biomarkers; D002236:Carbohydrate Conformation; D002277:Carcinoma / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005227:Fatty Acids / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology; D008175:Lung Neoplasms / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology*; D008264:Macrophages; D015394:Molecular Structure; D009000:Monocytes; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D012240:Rhodococcus / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D014199:Trehalose / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D015415:Biomarkers; D005227:Fatty Acids; D006017:Glycolipids; D013386:Succinates; D012967:Sodium Dodecyl Sulfate; D019802:Succinic Acid; D014199:Trehalose