title: Osteoblast adhesion to orthopaedic implant alloys: effects of cell adhesion molecules and diamond-like carbon coating.
abstract: In total joint arthroplasty, long-term outcomes depend in part on the biocompatibility of implant alloys. This study analyzed effects of surface finish and diamond-like carbon coating on osteoblast cell adhesion to polished titanium-aluminum-vanadium and polished or grit-blasted cobalt-chromium-molybdenum alloys. Osteoblast binding was tested in the presence and absence of the cell adhesion proteins fibronectin, laminin, fibrinogen, and vitronectin and was quantified by measurement of DNA content. Although adherence occurred in serum-free medium, maximal osteoblast binding required serum and was similar for titanium and cobalt alloys at 2 and 12 hours. With the grit-blasted cobalt alloy, cell binding was reduced 48% (p < 0.05) by 24 hours. Coating the alloys with diamond-like carbon did not alter osteoblast adhesion, whereas fibronectin pretreatment increased cell binding 2.6-fold (p < 0.05). In contrast, fibrinogen, vitronectin, and laminin did not enhance cell adhesion. These results support the hypothesis that cell adhesion proteins can modify cell binding to orthopaedic alloys. Although osteoblast binding was not affected by the presence of diamond-like carbon, this coating substance may influence other longer term processes, such as bone formation, and deserves further study.
authors.affiliation: Department of Function Restoration, Stanford University School of Medicine, California, USA.
authors.forename: R
authors.initials: R
authors.lastname: Kornu
authors.forename: W J
authors.initials: WJ
authors.lastname: Maloney
authors.forename: M A
authors.initials: MA
authors.lastname: Kelly
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
mesh_terms: D000497:Alloys; D000818:Animals; D001769:Blood; D002244:Carbon; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002460:Cell Line; D016895:Culture Media, Serum-Free; D018130:Diamond; D008670:Metals; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009984:Orthopedic Fixation Devices; D010006:Osteoblasts / Q000502:physiology* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D000497:Alloys; D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D008670:Metals; D002244:Carbon; D018130:Diamond