Similarity in genome organization between Molluscum contagiosum virus (MCV) and vaccinia virus (VV): identification of MCV homologues of the VV genes for protein kinase 2, structural protein VP8, RNA polymerase 35 kDa subunit and 3beta-hydroxysteroid dehydrogenase.
Molluscum contagiosum virus (MCV) and vaccinia virus (VV) are serologically unrelated poxviruses with a disparate genome composition (MCV, 66% G+C; VV, 33% G+C). Molecular studies of MCV have been hindered by the inability to propagate the virus in cells cultured in vitro. We sequenced 7765 bp of MCV DNA cloned from four widely spaced regions throughout the MCV genome and identified a total of 11 potential open reading frames (ORF), designated CX1-11. These include MCV homologues of the VV genes encoding protein kinase 2, structural protein VP8, RNA polymerase 35 kDa subunit and 3beta-hydroxysteroid dehydrogenase. The position and orientation of the MCV ORFs was collinear to the VV genome, with the exception of the region around ORF CX11 which is inverted in the MCV genome.
Douglass
N J
NJ
Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.
Blake
N W
NW
Cream
J J
JJ
Soteriou
B A
BA
Zhang
H Y
HY
Theodoridou
A
A
Archard
L C
LC
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D008976:Molluscum Contagiosum / Q000473:pathology / Q000821:virology*; D008977:Molluscum contagiosum virus / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins; D015678:Viral Structural Proteins / Q000235:genetics*
D036022:Capsid Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C108986:vaccinia virus protein VP8; D015096:3-Hydroxysteroid Dehydrogenases; C095546:protein kinase 2, Vaccinia virus; D017346:Protein Serine-Threonine Kinases; D012321:DNA-Directed RNA Polymerases