title: Purification and characterization of a lectin from Amaranthus hypochondriacus var. Mexico seeds.
abstract: A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ozeki
authors.forename: K
authors.initials: K
authors.lastname: Kamemura
authors.forename: K
authors.initials: K
authors.lastname: Moriyama
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Furuichi
authors.forename: H
authors.initials: H
authors.lastname: Umekawa
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006241:Haptens / Q000032:analysis; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D006241:Haptens; D037102:Lectins; D037121:Plant Lectins