Numerical and experimental dosimetry of Petri dish exposure setups.
Crawford TEM cells are often used to expose cell cultures or small animals in order to study the effects caused by high-frequency fields. They are self-contained, easy-to-use setups that provide a rather homogeneous field distribution in a large area around its center, corresponding approximately to far-field conditions. However, a number of conditions must be met if such TEM cells are intended to be used for in vitro experiments. For instance, poor interaction with the incident field must be maintained to avoid significant field disturbances in the TEM cell. This is best achieved with E-polarization, i.e., when the E-field vector is normal to the investigated cell layer lining the bottom of a synthetic Petri dish. In addition, E-polarization provides the most homogeneous field distribution of all polarizations within the entire layer of cells. In this paper, we present a detailed dosimetric assessment for 60 and 100 mm Petri dishes as well as for a 48-well titer plate at 835 MHz. The dosimetry was performed by using numerical computations. The modeling and the simplifications are validated by a second numerical technique and by experimental measurements. For thin liquid layers, an approximation formula is provided with which the induced field strength for many other experiments conducted in Petri dishes can be assessed reliably.
Burkhardt
M
M
Swiss Federal Institute of Technology, Zurich, Switzerland.
Poković
K
K
Gnos
M
M
Schmid
T
T
Kuster
N
N
D000042:Absorption; D000465:Algorithms; D000818:Animals; D018929:Cell Culture Techniques; D002477:Cells / Q000528:radiation effects*; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation; D004574:Electromagnetic Fields / Q000009:adverse effects; D006801:Humans; D008954:Models, Biological; D011829:Radiation Dosage; D015203:Reproducibility of Results; D013821:Thermometers
D003470:Culture Media