[Structure of O-specific polysaccharide from Proteus mirabilis O10, containing a new component of O-antigens--L-altruronic acid].
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O10 and studied after full acid hydrolysis and carboxyl reduction by 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the polysaccharide contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [sequence: see text]
Shashkov
A S
AS
Center of Microbiology and Virology, Polish Academy of Sciences, Lodowa.
Senchenkova
S N
SN
Toukach
F V
FV
Ziolkowski
A
A
Paramonov
N A
NA
Kaca
W
W
Knirel'
Iu A
IuA
Kochetkova
N K
NK
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry*
D019081:O Antigens; D014574:Uronic Acids; C102528:altruronic acid