title: Phenol-oxidizing enzymes: mechanisms and applications in biosensors.
abstract: Phenolic compounds are widely distributed in nature. Enzymes which catalyze their oxidation are monophenol monooxygenases, such as tyrosinases and laccases, and peroxidases. Their metabolic role includes the decomposition of natural complex aromatic polymers as well as polymerization of the oxidation products and the degradation of xenobiotics. Their catalytic properties and broad availability gained impact on the development of biosenors for both environmentally important pollutants and clinically relevant metabolites. Mechanisms for the phenol-oxidizine enzymes tyrosinases, laccases, and peroxidases are reviewed and some examples for their use in the construction of phenol selective biosenors are given.
authors.affiliation: Institut für Organische Chemie und Strukturanalytik, Universität Potsdam, Germany.
authors.forename: M G
authors.initials: MG
authors.lastname: Peter
authors.forename: U
authors.initials: U
authors.lastname: Wollenberger
mesh_terms: D015374:Biosensing Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D042845:Laccase; D008956:Models, Chemical; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000378:metabolism*; D013379:Substrate Specificity; D014443:Tyrosine / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005609:Free Radicals; D010636:Phenols; D014443:Tyrosine; D010088:Oxidoreductases; D042845:Laccase; D009195:Peroxidase; D014442:Monophenol Monooxygenase