Genetic analysis of the cause of gastroschisis in the HLG mouse strain.
An inbred mouse strain HLG shows a high incidence of gastroschisis after X-ray exposure to the zygotes. About 11% of the fetuses display this malformation after irradiation with 1 Gy. The C57BL-strain does not show the increased frequency of gastroschisis after radiation-exposure to the zygotes. The genetic background of this malformation was investigated in a backcross of HLG x C57BL females to HLG males. The pregnant HLG x C57BL females were irradiated in a stage in which the (HLG x C57BL) x HLG [BC1] embryos were in the 1-cell stage. The frequency of gastroschisis in the BC1 generation was compared with a genetic model of a single recessive mutation with 11% penetrance. This frequency does not fit a single-locus inheritance. The number of loci involved was estimated to be about two or three. HLG mouse strain may be a valuable animal model in the study of polygenic traits.
Hillebrandt
S
S
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Streffer
C
C
Müller
W U
WU
D000009:Abdominal Muscles / Q000002:abnormalities* / Q000196:embryology / Q000528:radiation effects*; D000016:Abnormalities, Radiation-Induced / Q000235:genetics* / Q000401:mortality; D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005313:Fetal Death / Q000235:genetics; D005333:Fetus / Q000528:radiation effects*; D006547:Hernia / Q000196:embryology / Q000235:genetics; D006720:Homozygote; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008957:Models, Genetic; D009154:Mutation; D011247:Pregnancy; D014965:X-Rays