title: A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: a controlled study.
abstract: OBJECTIVE
  To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
  
  
  METHODS
  Controlled clinical study.
  
  
  METHODS
  IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
  
  
  METHODS
  Patients with poor embryo quality in their previous IVF-ET cycles.
  
  
  METHODS
  Embryos from 40 patients, in which > 50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24-48 hours) before being replaced in the uterine cavity.
  
  
  METHODS
  Embryo quality.
  
  
  RESULTS
  Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
  
  
  CONCLUSIONS
  This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.
authors.affiliation: Department of Obstetrics and Gynecology, Carmel Medical Center Haifa, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Dirnfeld
authors.forename: S
authors.initials: S
authors.lastname: Goldman
authors.forename: Y
authors.initials: Y
authors.lastname: Gonen
authors.forename: M
authors.initials: M
authors.lastname: Koifman
authors.forename: I
authors.initials: I
authors.lastname: Calderon
authors.forename: H
authors.initials: H
authors.lastname: Abramovici
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000502:physiology*; D006801:Humans; D009924:Organ Culture Techniques; D011247:Pregnancy