Effect of buflomedil on the neutrophil-endothelial cell interaction under inflammatory and hypoxia conditions.
In hypoxia/ischaemia and ischaemia/reperfusion, human neutrophils are likely to play an important role in the development of endothelial cell damage in the microcirculation. Buflomedil hypochloride improves the capillary perfusion in such related situations, evoking a possible effect upon neutrophils. Using in vitro models of cell adhesion, buflomedil decreased 100% of histamine related neutrophil adhesion (flow system) and partially inhibited adhesion after IL-1-4 hours (flow and stable systems). Hypoxia induced neutrophil adhesion (4 hours) was also reduced by buflomedil, which decreased the expression of P-selectin at the surface of endothelial cells. As adenosine (NECA) exhibited the same results in hypoxia and theophylline inhibited them, such results support an action of buflomedil presumably via the A2 receptor.
Boisseau
M R
MR
University Victor Segalen, Bordeaux, France.
Pruvost
A
A
Renard
M
M
Closse
C
C
Belloc
F
F
Seigneur
M
M
Maurel
A
A
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D058669:Adrenergic alpha-2 Receptor Antagonists; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D019007:P-Selectin / Q000096:biosynthesis; D011759:Pyrrolidines / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology; D014471:Umbilical Veins
D058669:Adrenergic alpha-2 Receptor Antagonists; D000317:Adrenergic alpha-Antagonists; D016207:Cytokines; D007375:Interleukin-1; D019007:P-Selectin; D011759:Pyrrolidines; D018799:Intercellular Adhesion Molecule-1; D019830:Adenosine-5'-(N-ethylcarboxamide); D013806:Theophylline; D000241:Adenosine; C010651:buflomedil