title: In vitro activity of ampicillin-sulbactam against clinical multiresistant Acinetobacter baumannii isolates.
abstract: We evaluated the in vitro activity of ampicillin-sulbactam in comparison with that of broad-spectrum antimicrobial agents against Acinetobacter baumannii isolates. Two hundred and twelve clinical isolates collected between January 1993 and March 1995 from two tertiary hospitals located in São Paulo, Brazil were tested for susceptibility by the disk diffusion method against several broad-spectrum antimicrobial agents, including imipenem, ciprofloxacin, ceftazidime, aztreonam, amikacin, and polymyxin B. All strains were susceptible to polymyxin B. The second most active compound was the combination ampicillin-sulbactam (88% susceptibility). Only 79% of the isolates were susceptible to imipenem. Ciprofloxacin was active against 60 (28%) and amikacin against 34 (16%) isolates. Ceftazidime was the most active cephalosporin; however, only 9% of the isolates were susceptible to this compound. Both aztreonam and ampicillin alone showed very poor activity against this species (1% susceptibility). The prevalence of severe infections due to A. baumannii is increasing very rapidly in the tertiary hospitals of São Paulo and there are very few options for the treatment of these infections. Polymyxin B is invariably in vitro active against this species; however, this compound can cause severe side effects and is not commercially available for intravenous use in Brazil and in several other countries. Our results indicated that the combination ampicillin-sulbactam may be an alternative drug for the treatment of infections due to multiresistant A. baumannii; however, further studies are necessary to evaluate the clinical role of this compound for the treatment of severe infections.
authors.affiliation: Division of Infectious Diseases, Universidade Federal de São Paulo, Escola Paulista de Medicina, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Gales
authors.forename: H S
authors.initials: HS
authors.lastname: Sader
authors.forename: S
authors.initials: S
authors.lastname: Sinto
authors.forename: O P
authors.initials: OP
authors.lastname: Santos
authors.forename: C M
authors.initials: CM
authors.lastname: Mendes
mesh_terms: D000150:Acinetobacter / Q000187:drug effects*; D000667:Ampicillin / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D004359:Drug Therapy, Combination / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013407:Sulbactam / Q000494:pharmacology
chemical_list: C035444:sultamicillin; D000667:Ampicillin; D013407:Sulbactam