Characterization of novel non-toxic Bacillus thuringiensis isolates from Korea.
Four Bacillus Thuringiensis isolates from soil samples produced parasporal inclusions which were non-toxic to insects. The isolates were named B. thuringiensis NTB-1, NTB-2, NTB-3 and NTB-4. The parasporal inclusions were shown to be ovoid by phase contrast and scanning electron microscopy. The serotypes of the four isolates were determined by agglutination using 33 antisera; NTB-1 and NTB-4 seemed to be subsp. israelensis, and NTB-2 seemed to be subsp. pondicheriensis. NTB-3 did not react with the 33 antisera. However, comparison of parasporal protein and plasmid DNA patterns of the four isolates with those of 15 known non-toxic B. thuringiensis strains demonstrated that the four isolates are novel.
Roh
J Y
JY
Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Park
H W
HW
Jin
B R
BR
Kim
H S
HS
Yu
Y M
YM
Kang
S K
SK
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007313:Insecta; D007723:Korea