Cryopreservation of infective larvae of Haemonchus contortus.
Three freeze protectants were evaluated to preserve H. contortus infective larvae. Freezing solutions used: A) saline solution phosphate buffer pH 7.2 (PBS); B) 10% DMSO (dimethyl sulphoxide); C) 10% glycerol. Fifty thousand infective larvae were put into each of 10 vials per freeze protectant and then stored into liquid nitrogen. Results were based on the motility of the larvae under a light microscope at 30, 90, 180, and 360 days of freezing. Ten vials of each freeze protectant were removed from the liquid nitrogen at these times and immediately were put on water at 37 C during a minute. Motility percentages obtained were as follows: PBS: 36%, 20%, 7% and 39%; DMSO,: 87%, 69%, 46% and 85%; glycerol: 67%, 62%, 29% and 55%; at 30, 90, 180 and 360 days respectively. Inoculation of infectiva larvae from DMSO and glycerol to calves was successful after 28 days. DMSO was a better freeze preserver for H. contortus.
Liébano-Hernández
E
E
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria INIFAP-SAGAR, Morelos, Mexico.
López-Arellano
M E
ME
Vázquez-Prats
V
V
Mendoza de Gives
P
P
D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006189:Haemonchus / Q000187:drug effects / Q000254:growth & development*; D007814:Larva; D011309:Preservation, Biological
D003451:Cryoprotective Agents; D005990:Glycerol; D004121:Dimethyl Sulfoxide