title: Strongly reduced phage Qbeta replication, but normal phage MS2 replication in an Escherichia coli K12 mutant with inactivated Qbeta host factor (hfq) gene.
abstract: RNA phage production was observed in Escherichia coli hfq mutant strains transformed with plasmids containing genomic cDNA of phages Qbeta or MS2. Qbeta production was reduced 5000-fold in an insertional mutant containing an omega cassette in the middle of hfq, but was unaffected in a mutant with the cassette near the end of hfq. The insertions had no effect on MS2 replication.
authors.affiliation: Institut für Molekularbiologie Abt. I, Universität Zürich, Zürich, 8093, Switzerland.
authors.forename: Q
authors.initials: Q
authors.lastname: Su
authors.forename: D
authors.initials: D
authors.lastname: Schuppli
authors.lastname: Tsui HcT
authors.forename: M E
authors.initials: ME
authors.lastname: Winkler
authors.forename: H
authors.initials: H
authors.lastname: Weber
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D003090:Coliphages / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000821:virology*; D029968:Escherichia coli Proteins; D035001:Host Factor 1 Protein; D034982:Integration Host Factors; D017909:Levivirus / Q000502:physiology*; D009154:Mutation; D012317:RNA Phages / Q000502:physiology*; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D035001:Host Factor 1 Protein; D034982:Integration Host Factors; C440502:integration host factor, E coli