Molecular cloning of a hemopexin-like cDNA from rainbow trout liver.
Hemopexin is an abundant heme-binding serum protein synthesized in the liver and for which the sequence is determined only for mammals. Little is known about fish heme-binding proteins. We have cloned a rainbow trout complementary DNA that encodes a 445 amino acid polypeptide exhibiting an overall 30% homology with human hemopexin and 69% with the goldfish warm temperature acclimation-related 65-kDa protein. Structural homology, deduced from hydrophobic cluster analysis (HCA), was strong between the trout and human proteins since global HCA scores of 76% were obtained when the N- or C-terminal halves of the two proteins were compared. Moreover, several characteristics of hemopexin were found in the trout protein; finally, the trout hemopexin-like messenger RNA was specifically expressed in the liver. We conclude that the trout protein is a good candidate for a true fish hemopexin.
Miot
S
S
Equipe d'Endocrinologie Moléculaire de la Reproduction, Centre National de la Recherche Scientifique URA 256, Université de Rennes 1, France.
Duval
J
J
Le Goff
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary; D006466:Hemopexin / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D006466:Hemopexin