Expression of mouse metallothionein in the cyanobacterium Synechococcus PCC7942.
A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUc303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacterium Synechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ion-binding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two- to five-fold increase in cadmium ion tolerance and accumulation on Synechococcus PCC7942.
Erbe
J L
JL
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham 35294, USA.
Taylor
K B
KB
Hall
L M
LM
D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014169:Transformation, Bacterial
D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D002104:Cadmium; D008668:Metallothionein; D015500:Chloramphenicol O-Acetyltransferase