Binding of recombinant pig zona pellucida protein 1 (ZP1) to acrosome-reacted spermatozoa.
In an immunofluorescent study, recombinant pig zona pellucida protein 1 (pZP1) produced in Escherichia coli bound to acrosome-reacted spermatozoa from five different mammals. The location of the binding site moved from the equatorial region to the midpiece and tail regions during the incubation. This finding suggests that pig ZP1 acts as a secondary sperm-binding receptor in an interspecies manner and assists penetration of the spermatozoa through the zona pellucida. The recombinant pZP1 bound to 55 kDa protein (proacrosin) and 40 kDa protein of the boar spermatozoa as revealed by immunoblotting.
Tsubamoto
H
H
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Japan.
Hasegawa
A
A
Inoue
M
M
Yamasaki
N
N
Koyama
K
K
D000176:Acrosin / Q000378:metabolism; D000177:Acrosome / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D004527:Egg Proteins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism; D005260:Female; D015151:Immunoblotting; D008297:Male; D008322:Mammals / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000378:metabolism; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D004792:Enzyme Precursors; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D000072216:Zona Pellucida Glycoproteins; C000606519:Zp1 protein, mouse; C022438:proacrosin; D000176:Acrosin