title: Effect of side groups on the action of beta-xylosidase from Trichoderma reesei against substituted xylo-oligosaccharides.
abstract: The action of beta-xylosidase from Trichoderma reesei against different substituted xylo-oligosaccharides was studied. The enzyme cleaved off all unsubstituted xylose units from the non-reducing end of 1,2-linked uronic acid substituted xylo-oligosaccharides. Surprisingly, an L-arabinofuranosyl group linked alpha-1,3 to the xylopyranosyl ring was found to protect the beta-1,4-xylosidic linkage before the substituted xylose unit from being cleaved by the beta-xylosidase. Most probably the 1,3-linked substituent sterically hinders the hydrolysis. According to the results of the present work, beta-xylosidase of T. reesei is not able to remove all unsubstituted xylose units from the non-reducing end of substituted xylo-oligosaccharides, as had been believed previously.
authors.affiliation: VTT Biotechnology and Food Research, Finland. Maija.Tenkanen@vtt.fi
authors.forename: M
authors.initials: M
authors.lastname: Tenkanen
authors.forename: E
authors.initials: E
authors.lastname: Luonteri
authors.forename: A
authors.initials: A
authors.lastname: Teleman
mesh_terms: D002852:Chromatography, Ion Exchange; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase