[A novel method of concentrating bacterial suspensions for transmission electron microscopy].
Presents a new method for making preparations of bacteria and their unculturable forms from suspensions with low concentrations of cells, from 1 x 108 to 1 x 104 per ml. to be examined under transmission electron microscope. The novelty of the method consists in the technique of concentrating bacteria by making a colloid solution consisting of bovine serum albumin and polyethyleneglycol. Associations of protein molecules, polyethyleneglycol, and bacteria, forming in the solution, are sedimented by centrifugation after 48-hour incubation at 4 degrees C. The solidity of the resultant macroscopic sediment is sufficient for studying the cells contained in it under transmission electron microscope.
Didenko
L V
LV
Konstantinova
N D
ND
Romanova
Iu M
IuM
Levina
G A
GA
Alekseeva
N V
NV
D001431:Bacteriological Techniques; D008854:Microscopy, Electron; D012486:Salmonella typhimurium / Q000254:growth & development / Q000648:ultrastructure*