Vasopressin modulates membrane properties of taste cells isolated from bullfrogs.
The effect of arginine vasopressin (AVP) on the membrane properties was analyzed in isolated bullfrog taste cells using a perforated whole-cell patch-clamp technique. AVP (100 nM) induced three kinds of responses in rod-type taste cells: appearance of inward current, inhibition of voltage ramp-induced outward current and enhancement of the outward current. The Ca(2+)-ionophore ionomycin (3 microM) also induced inward current in taste cells. A membrane-permeable cAMP analog, 8-CPT-cAMP (0.3 mM) inhibited voltage ramp-induced outward current in some rod cells, but enhanced the current in other rod cells. The results suggest that AVP may increase either intracellular Ca2+ level or cAMP level in taste cells, modulating the membrane excitability.
Okada
Y
Y
Department of Physiology, Nagasaki University School of Dentistry, Japan.
Fujiyama
R
R
Miyamoto
T
T
Sato
T
T
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D011892:Rana catesbeiana; D013650:Taste Buds / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013873:Thionucleotides / Q000494:pharmacology
D007476:Ionophores; D013873:Thionucleotides; D001127:Arginine Vasopressin; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D015759:Ionomycin; D000242:Cyclic AMP