The effect of topical steroid on the actions of vasoconstrictor and vasodilator peptides in human skin.
The effect of topical application of clobetasol propionate ointment (0.05% w/v) on the vascular changes induced by intradermal injections of histamine, calcitonin gene-related peptide, substance P, endothelin-1 and compound 48/80 was studied. Clobetasol propionate ointment was applied topically under occlusion to the forearm skin of healthy volunteers and vehicle base was applied to the contralateral forearm. The intradermal injections were made 4 h or, in a separate study, 72 h after topical steroid application. Responses were measured by planimetry and laser Doppler flowmetry. Four hours application of steroid did not significantly alter the responses to any of the vasoactive substances. After 72 hours application, clobetasol propionate significantly increased the size of the endothelin-1-induced area of vasoconstriction (p < 0.02) and significantly reduced the size of the flares induced by endothelin-1 (p < 0.02), substance P (p < 0.009) and compound 48/80 (p < 0.05). We conclude that the most likely explanation of our data is an inhibition by the steroid of cutaneous mast cell function.
Goldsmith
P
P
Department of Dermatology, University College London Medical School, UK.
Bunker
C
C
Leslie
T
T
Foreman
J
J
Dowd
P M
PM
D000287:Administration, Topical; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002990:Clobetasol / Q000031:analogs & derivatives / Q000494:pharmacology; D019332:Endothelin-1 / Q000494:pharmacology; D004890:Erythema / Q000139:chemically induced; D005260:Female; D005938:Glucocorticoids; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009824:Ointments; D010455:Peptides / Q000494:pharmacology*; D012867:Skin / Q000098:blood supply* / Q000187:drug effects*; D013373:Substance P / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D019332:Endothelin-1; D005938:Glucocorticoids; D009824:Ointments; D010455:Peptides; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D002990:Clobetasol; D015740:Calcitonin Gene-Related Peptide