Projection structure of a gap junction membrane channel at 7 A resolution.
Electron cryo-microscopy and image analysis of frozen-hydrated, two-dimensional crystals of gap junction membrane channels formed by recombinant alpha 1 connexin (Cx43) reveal a ring of transmembrane alpha-helices that lines the aqueous pore and a second ring of alpha-helices in close contact with the membrane lipids.
Unger
V M
VM
Kumar
N M
NM
Gilula
N B
NB
Yeager
M
M
D000818:Animals; D002460:Cell Line; D018031:Connexin 43 / Q000737:chemistry* / Q000378:metabolism; D006224:Cricetinae; D018360:Crystallography, X-Ray; D008563:Membrane Lipids / Q000378:metabolism; D008854:Microscopy, Electron / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D018031:Connexin 43; D008563:Membrane Lipids; D011994:Recombinant Proteins