title: Human anti-IgE monoclonal antibody blocks passive sensitization of human and rhesus monkey bladder.
abstract: IgE antibodies may play an important role in noninfectious urinary inflammation, including interstitial cystitis (IC). In this study, urinary bladder strips were passively sensitized for 20 hours with serum from a ragweed-sensitive patient in the absence or presence of an anti-human IgE monoclonal antibody (MaE11) at 0, 1-, or 5-fold IgE concentration. The urinary bladder strips then were suspended in a superfusion apparatus for measurement of contraction and histamine release in response to antigen E (AgE) challenge. Non-sensitized tissues did not react to AgE challenge, whereas AgE challenge of passively-sensitized tissues resulted in time-dependent bladder contraction and histamine release. MaE11 abolished AgE-induced contraction and histamine release in a concentration-dependent manner. The safety of MaE11 was confirmed by its failure to contract or release histamine from passively-sensitized bladder tissues. The results of this study suggest that MaE11 may have immunotherapeutic benefit for amelioration of IgE-mediated diseases of the urinary system.
authors.affiliation: Department of Surgical Sciences, School of Veterinary Medicine University of Wisconsin, Madison 53706, USA.
authors.forename: R
authors.initials: R
authors.lastname: Saban
authors.forename: M
authors.initials: M
authors.lastname: Haak-Frendscho
authors.forename: M
authors.initials: M
authors.lastname: Zine
authors.forename: L G
authors.initials: LG
authors.lastname: Presta
authors.forename: D E
authors.initials: DE
authors.lastname: Bjorling
authors.forename: P
authors.initials: P
authors.lastname: Jardieu
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D006636:Histamine Release / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D001743:Urinary Bladder / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E