Structure of an ovine interferon receptor and its expression in endometrium.
A sheep type I interferon receptor (oIFNAR1) cDNA was isolated from a lambda-ZAP library using a reverse transcription (RT)-PCR product probe generated from oestrous endometrial RNA. The oIFNAR1 cDNA was 79, 66 and 95% homologous to human, murine and bovine IFNAR1 cDNAs respectively. The encoded receptor was a 560-amino acid transmembrane protein 80, 66 and 95% similar to human, murine and bovine IFNAR1 respectively. Northern blot analysis of endometrial mRNA revealed the presence of 6.5, 4.3 and 3.7 kb transcripts. Using semi-quantitative RT-PCR the oIFNAR1 mRNA was not found to be down-regulated after 72 h treatment with bovine recombinant IFN-alpha I in in vitro experiments with endometrial explants.
Kaluz
S
S
Department of Physiology, University of Nottingham, Loughborough, Leicestershire, UK.
Fisher
P A
PA
Kaluzova
M
M
Sheldrick
E L
EL
Flint
A P
AP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017471:Receptors, Interferon / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D018076:DNA, Complementary; D016898:Interferon-alpha; D012333:RNA, Messenger; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases