Determination of single-stranded oligodeoxynucleotides by capillary gel electrophoresis with laser induced fluorescence and on column derivatization.
OliGreen reagent, a new dye for complexing of oligonucleotides of low molecular mass was successfully utilized for their quantitative analysis using on column derivatization and capillary gel electrophoresis with laser induced fluorescence. Method optimization for the precise, accurate and reproducible quantitation of low-molecular-mass oligonucleotides from aqueous matrices is described.
Reyderman
L
L
University of Texas at Austin, College of Pharmacy, Division of Pharmaceutics 78712, USA.
Stavchansky
S
S
D004396:Coloring Agents; D004277:DNA, Single-Stranded / Q000032:analysis*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D009691:Nucleic Acid Denaturation; D015203:Reproducibility of Results
D004396:Coloring Agents; D004277:DNA, Single-Stranded