[Embryonal metabolism].
It is very difficult to have a clear and homogeneous idea of the embryo metabolism. In fact it may vary from one species to another and also according to the embryonic stage: i.e. before and after genomic activation. Basic compounds such as glucose may be toxic, but obviously, it is more the problem of the quantity introduced in the culture media and an unsuitable balance between the metabolites which may impair the embryonic development. At low concentration glucose is actively metabolised by embryos. High levels of amino acids are deleterious (due to release of ammonia), but they are necessary at low concentrations. Addition of serum or other biological fluids is generally useless. Further knowledge on embryo metabolism is necessary to avoid culture medium related delay or developmental blocks. Sequential media are at least partly the answer.
Khatchadourian
C
C
INSA Unité de Biologie du développement pré-implantatoire, UA INRA 203, Villeurbanne.
Joly
C
C
Ménézo
Y
Y
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D050356:Lipid Metabolism; D009694:Nucleic Acid Precursors
D000596:Amino Acids; D003470:Culture Media; D009694:Nucleic Acid Precursors