Detection of protein A produced by Staphylococcus aureus with a fiber-optic-based biosensor.
Staphylococcus aureus is a pathogen important in causing human infections and intoxication. A sensitive fiber-optic that produces evanescent waves was developed for the detection of protein A, a product secreted only by S. aureus. In the immunosensor, a 40-mV argon-ion laser that generated laser light at 488 nm was used together with plastic optical fiber and antibodies to protein A were physically adsorbed onto the fiber. The principle of the detection involved a sandwich immunoassay with fluorescein isothiocyanate conjugated with anti-(protein A) immunoglobulin G to produce signals of the antigen-antibody reaction. The detection limit was 1 ng of protein A per milliliter. The fiber-optic immunosensor could be used for rapid and specific detection of S. aureus in clinical specimens and foods.
Chang
Y H
YH
School of Medical Technology, National Yang Ming University, Taipei, Taiwan, Republic of China.
Chang
T C
TC
Kao
E F
EF
Chou
C
C
D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D005516:Food Microbiology; D006801:Humans; D055100:Optical Fibers; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000378:metabolism*; D013997:Time Factors
D013205:Staphylococcal Protein A