title: Difference in behavior between responses to forskolin and general odorants in turtle vomeronasal organ.
abstract: To elucidate the signal transduction mechanisms in the turtle vomeronasal receptor neurons, the effects of forskolin, changes in mucosal Ca2+ concentrations and ruthenium red on the responses of the accessory olfactory bulb to general odorants were examined. Forskolin elicited a large response, suggesting that there are cAMP-gated channels in the vomeronasal neurons. On the other hand, the dependence of the responses to general odorants on Ca2+ concentrations was different from that of the response to forskolin. A large response to an odorant (n-amyl acetate) appeared after the cAMP-mediated pathway was fully desensitized by application of 50 microM forskolin. These results suggest that the cAMP-mediated pathway does not contribute significantly to generation of the response to general odorants. A concentration of 50 microM ruthenium red significantly reduced the responses to n-amyl acetate alone and after 50 microM forskolin desensitization, suggesting that the inositol triphosphate-mediated pathway contributes partly to generation of the responses to general odorants in the vomeronasal neurons.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Kanaki
authors.forename: M
authors.initials: M
authors.lastname: Kashiwayanagi
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology; D004594:Electrophysiology; D015640:Ion Channel Gating; D009812:Odorants; D009830:Olfactory Bulb / Q000187:drug effects / Q000502:physiology; D000439:Pentanols / Q000494:pharmacology; D012430:Ruthenium Red / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D014426:Turtles / Q000502:physiology*; D019147:Vomeronasal Organ / Q000187:drug effects / Q000502:physiology*
chemical_list: D000439:Pentanols; D012430:Ruthenium Red; D005576:Colforsin; C005716:amyl acetate; D000242:Cyclic AMP; D002118:Calcium